LOCUS       DADLSE010000001       120006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLSE010000001 DADLSE010000000
VERSION     DADLSE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 120006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.001"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..326
                     /locus_tag="JN692_000001"
     CDS             <1..326
                     /locus_tag="JN692_000001"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7262421.1"
                     /translation="GLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYN
                     LGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLK
                     HANHK"
     gene            complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN692_000002"
     CDS             complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN692_000002"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7262422.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            1109..3499
                     /locus_tag="JN692_000003"
     CDS             1109..3499
                     /locus_tag="JN692_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7262423.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN692_000004"
     CDS             complement(3546..5096)
                     /gene="cueO"
                     /locus_tag="JN692_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HAY7262424.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVRLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            5262..5609
                     /locus_tag="JN692_000005"
     CDS             5262..5609
                     /locus_tag="JN692_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7262425.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            5715..6581
                     /gene="speE"
                     /locus_tag="JN692_000006"
     CDS             5715..6581
                     /gene="speE"
                     /locus_tag="JN692_000006"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7262426.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            6597..7391
                     /gene="speD"
                     /locus_tag="JN692_000007"
     CDS             6597..7391
                     /gene="speD"
                     /locus_tag="JN692_000007"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7262427.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(7429..7791)
                     /locus_tag="JN692_000008"
     CDS             complement(7429..7791)
                     /locus_tag="JN692_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7262428.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN692_000009"
     CDS             complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN692_000009"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7262429.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            10918..12771
                     /locus_tag="JN692_000010"
     CDS             10918..12771
                     /locus_tag="JN692_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7262430.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQASAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRLER"
     gene            complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN692_000011"
     CDS             complement(12842..14266)
                     /gene="lpdA"
                     /locus_tag="JN692_000011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7262431.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(14591..16483)
                     /gene="aceF"
                     /locus_tag="JN692_000012"
     CDS             complement(14591..16483)
                     /gene="aceF"
                     /locus_tag="JN692_000012"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7262432.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(16498..19161)
                     /gene="aceE"
                     /locus_tag="JN692_000013"
     CDS             complement(16498..19161)
                     /gene="aceE"
                     /locus_tag="JN692_000013"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7262433.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19322..20086)
                     /gene="pdhR"
                     /locus_tag="JN692_000014"
     CDS             complement(19322..20086)
                     /gene="pdhR"
                     /locus_tag="JN692_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7262434.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            20627..22000
                     /gene="aroP"
                     /locus_tag="JN692_000015"
     CDS             20627..22000
                     /gene="aroP"
                     /locus_tag="JN692_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7262435.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(22043..22897)
                     /gene="ampE"
                     /locus_tag="JN692_000016"
     CDS             complement(22043..22897)
                     /gene="ampE"
                     /locus_tag="JN692_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7262436.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(22894..23445)
                     /gene="ampD"
                     /locus_tag="JN692_000017"
     CDS             complement(22894..23445)
                     /gene="ampD"
                     /locus_tag="JN692_000017"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7262437.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            23533..24426
                     /gene="nadC"
                     /locus_tag="JN692_000018"
     CDS             23533..24426
                     /gene="nadC"
                     /locus_tag="JN692_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7262438.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLTENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            24629..25069
                     /gene="ppdD"
                     /locus_tag="JN692_000019"
     CDS             24629..25069
                     /gene="ppdD"
                     /locus_tag="JN692_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7262439.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            25079..26464
                     /gene="gspE"
                     /locus_tag="JN692_000020"
     CDS             25079..26464
                     /gene="gspE"
                     /locus_tag="JN692_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7262440.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            26454..27656
                     /gene="hofC"
                     /locus_tag="JN692_000021"
     CDS             26454..27656
                     /gene="hofC"
                     /locus_tag="JN692_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7262441.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(27691..28734)
                     /gene="guaC"
                     /locus_tag="JN692_000022"
     CDS             complement(27691..28734)
                     /gene="guaC"
                     /locus_tag="JN692_000022"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7262442.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            28959..29579
                     /gene="coaE"
                     /locus_tag="JN692_000023"
     CDS             28959..29579
                     /gene="coaE"
                     /locus_tag="JN692_000023"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7262443.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            29579..30322
                     /gene="zapD"
                     /locus_tag="JN692_000024"
     CDS             29579..30322
                     /gene="zapD"
                     /locus_tag="JN692_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7262444.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            30332..30529
                     /gene="yacG"
                     /locus_tag="JN692_000025"
     CDS             30332..30529
                     /gene="yacG"
                     /locus_tag="JN692_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7262445.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(30629..31018)
                     /gene="mutT"
                     /locus_tag="JN692_000026"
     CDS             complement(30629..31018)
                     /gene="mutT"
                     /locus_tag="JN692_000026"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7262446.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(31078..33783)
                     /gene="secA"
                     /locus_tag="JN692_000027"
     CDS             complement(31078..33783)
                     /gene="secA"
                     /locus_tag="JN692_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7262447.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGKRKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(33845..34357)
                     /gene="secM"
                     /locus_tag="JN692_000028"
     CDS             complement(33845..34357)
                     /gene="secM"
                     /locus_tag="JN692_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7262448.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(34587..35504)
                     /gene="lpxC"
                     /locus_tag="JN692_000029"
     CDS             complement(34587..35504)
                     /gene="lpxC"
                     /locus_tag="JN692_000029"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7262449.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(35605..36756)
                     /gene="ftsZ"
                     /locus_tag="JN692_000030"
     CDS             complement(35605..36756)
                     /gene="ftsZ"
                     /locus_tag="JN692_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7262450.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(36817..38079)
                     /gene="ftsA"
                     /locus_tag="JN692_000031"
     CDS             complement(36817..38079)
                     /gene="ftsA"
                     /locus_tag="JN692_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7262451.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(38076..38906)
                     /gene="ftsQ"
                     /locus_tag="JN692_000032"
     CDS             complement(38076..38906)
                     /gene="ftsQ"
                     /locus_tag="JN692_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7262452.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(38908..39828)
                     /locus_tag="JN692_000033"
     CDS             complement(38908..39828)
                     /locus_tag="JN692_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase B"
                     /protein_id="HAY7262453.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(39821..41296)
                     /gene="murC"
                     /locus_tag="JN692_000034"
     CDS             complement(39821..41296)
                     /gene="murC"
                     /locus_tag="JN692_000034"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7262454.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(41350..42417)
                     /gene="murG"
                     /locus_tag="JN692_000035"
     CDS             complement(41350..42417)
                     /gene="murG"
                     /locus_tag="JN692_000035"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7262455.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(42414..43658)
                     /gene="ftsW"
                     /locus_tag="JN692_000036"
     CDS             complement(42414..43658)
                     /gene="ftsW"
                     /locus_tag="JN692_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7262456.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(43658..44974)
                     /gene="murD"
                     /locus_tag="JN692_000037"
     CDS             complement(43658..44974)
                     /gene="murD"
                     /locus_tag="JN692_000037"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7262457.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(44977..46059)
                     /gene="mraY"
                     /locus_tag="JN692_000038"
     CDS             complement(44977..46059)
                     /gene="mraY"
                     /locus_tag="JN692_000038"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7262458.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(46053..47411)
                     /gene="murF"
                     /locus_tag="JN692_000039"
     CDS             complement(46053..47411)
                     /gene="murF"
                     /locus_tag="JN692_000039"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7262459.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(47408..48895)
                     /gene="murE"
                     /locus_tag="JN692_000040"
     CDS             complement(47408..48895)
                     /gene="murE"
                     /locus_tag="JN692_000040"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7262460.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(48882..50648)
                     /locus_tag="JN692_000041"
     CDS             complement(48882..50648)
                     /locus_tag="JN692_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7262461.1"
                     /translation="MKAAVKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGTIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(50664..51029)
                     /gene="ftsL"
                     /locus_tag="JN692_000042"
     CDS             complement(50664..51029)
                     /gene="ftsL"
                     /locus_tag="JN692_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7262462.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(51026..51967)
                     /gene="rsmH"
                     /locus_tag="JN692_000043"
     CDS             complement(51026..51967)
                     /gene="rsmH"
                     /locus_tag="JN692_000043"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7262463.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(51969..52427)
                     /gene="mraZ"
                     /locus_tag="JN692_000044"
     CDS             complement(51969..52427)
                     /gene="mraZ"
                     /locus_tag="JN692_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7262464.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(53029..54033)
                     /gene="cra"
                     /locus_tag="JN692_000045"
     CDS             complement(53029..54033)
                     /gene="cra"
                     /locus_tag="JN692_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7262465.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(54115..54201)
                     /locus_tag="JN692_000046"
     CDS             complement(54115..54201)
                     /locus_tag="JN692_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7262466.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(54213..54704)
                     /gene="ilvN"
                     /locus_tag="JN692_000047"
     CDS             complement(54213..54704)
                     /gene="ilvN"
                     /locus_tag="JN692_000047"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7262467.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDQTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(54707..56431)
                     /gene="ilvI"
                     /locus_tag="JN692_000048"
     CDS             complement(54707..56431)
                     /gene="ilvI"
                     /locus_tag="JN692_000048"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7262468.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPFVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(56749..57693)
                     /gene="leuO"
                     /locus_tag="JN692_000049"
     CDS             complement(56749..57693)
                     /gene="leuO"
                     /locus_tag="JN692_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7262469.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPS
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            58353..58439
                     /gene="leuL"
                     /locus_tag="JN692_000050"
     CDS             58353..58439
                     /gene="leuL"
                     /locus_tag="JN692_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7262470.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            58532..60103
                     /gene="leuA"
                     /locus_tag="JN692_000051"
     CDS             58532..60103
                     /gene="leuA"
                     /locus_tag="JN692_000051"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7262471.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            60103..61194
                     /gene="leuB"
                     /locus_tag="JN692_000052"
     CDS             60103..61194
                     /gene="leuB"
                     /locus_tag="JN692_000052"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7262472.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            61197..62597
                     /gene="leuC"
                     /locus_tag="JN692_000053"
     CDS             61197..62597
                     /gene="leuC"
                     /locus_tag="JN692_000053"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7262473.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKIMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            62608..63213
                     /gene="leuD"
                     /locus_tag="JN692_000054"
     CDS             62608..63213
                     /gene="leuD"
                     /locus_tag="JN692_000054"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7262474.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(63262..64440)
                     /gene="setA"
                     /locus_tag="JN692_000055"
     CDS             complement(63262..64440)
                     /gene="setA"
                     /locus_tag="JN692_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7262475.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(64542..64673)
                     /gene="sgrT"
                     /locus_tag="JN692_000056"
     CDS             complement(64542..64673)
                     /gene="sgrT"
                     /locus_tag="JN692_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7262476.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            64762..66417
                     /locus_tag="JN692_000057"
     CDS             64762..66417
                     /locus_tag="JN692_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7262477.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEMPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      66451..66550
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            66581..67564
                     /gene="thiB"
                     /locus_tag="JN692_000058"
     CDS             66581..67564
                     /gene="thiB"
                     /locus_tag="JN692_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7262478.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWLVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESNLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            67540..69150
                     /gene="thiP"
                     /locus_tag="JN692_000059"
     CDS             67540..69150
                     /gene="thiP"
                     /locus_tag="JN692_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7262479.1"
                     /translation="MATRRQPLIPGWLIPSVSAATLVVAVAIAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            69134..69832
                     /gene="thiQ"
                     /locus_tag="JN692_000060"
     CDS             69134..69832
                     /gene="thiQ"
                     /locus_tag="JN692_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7262480.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(69946..70710)
                     /locus_tag="JN692_000061"
     CDS             complement(69946..70710)
                     /locus_tag="JN692_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7262481.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(70796..71674)
                     /gene="araC"
                     /locus_tag="JN692_000062"
     CDS             complement(70796..71674)
                     /gene="araC"
                     /locus_tag="JN692_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7262482.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            72013..73713
                     /gene="araB"
                     /locus_tag="JN692_000063"
     CDS             72013..73713
                     /gene="araB"
                     /locus_tag="JN692_000063"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HAY7262483.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            73724..75226
                     /gene="araA"
                     /locus_tag="JN692_000064"
     CDS             73724..75226
                     /gene="araA"
                     /locus_tag="JN692_000064"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7262484.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYNDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            75426..76121
                     /gene="araD"
                     /locus_tag="JN692_000065"
     CDS             75426..76121
                     /gene="araD"
                     /locus_tag="JN692_000065"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7262485.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            76196..78547
                     /gene="polB"
                     /locus_tag="JN692_000066"
     CDS             76196..78547
                     /gene="polB"
                     /locus_tag="JN692_000066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7262486.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            78712..81618
                     /gene="rapA"
                     /locus_tag="JN692_000067"
     CDS             78712..81618
                     /gene="rapA"
                     /locus_tag="JN692_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7262487.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            81630..82289
                     /gene="rluA"
                     /locus_tag="JN692_000068"
     CDS             81630..82289
                     /gene="rluA"
                     /locus_tag="JN692_000068"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7262488.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(82406..83221)
                     /gene="djlA"
                     /locus_tag="JN692_000069"
     CDS             complement(82406..83221)
                     /gene="djlA"
                     /locus_tag="JN692_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7262489.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            83476..85830
                     /gene="lptD"
                     /locus_tag="JN692_000070"
     CDS             83476..85830
                     /gene="lptD"
                     /locus_tag="JN692_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7262490.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEALGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            85883..87169
                     /gene="surA"
                     /locus_tag="JN692_000071"
     CDS             85883..87169
                     /gene="surA"
                     /locus_tag="JN692_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7262491.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDTTLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRGRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQLNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            87169..88158
                     /gene="pdxA"
                     /locus_tag="JN692_000072"
     CDS             87169..88158
                     /gene="pdxA"
                     /locus_tag="JN692_000072"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7262492.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            88155..88976
                     /gene="rsmA"
                     /locus_tag="JN692_000073"
     CDS             88155..88976
                     /gene="rsmA"
                     /locus_tag="JN692_000073"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7262493.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDHFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            88979..89356
                     /gene="apaG"
                     /locus_tag="JN692_000074"
     CDS             88979..89356
                     /gene="apaG"
                     /locus_tag="JN692_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7262494.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVHGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            89363..90205
                     /gene="apaH"
                     /locus_tag="JN692_000075"
     CDS             89363..90205
                     /gene="apaH"
                     /locus_tag="JN692_000075"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7262495.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(90283..90762)
                     /gene="folA"
                     /locus_tag="JN692_000076"
     CDS             complement(90283..90762)
                     /gene="folA"
                     /locus_tag="JN692_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7262496.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(90953..92814)
                     /gene="kefC"
                     /locus_tag="JN692_000077"
                     /pseudo
     CDS             complement(90953..92814)
                     /gene="kefC"
                     /locus_tag="JN692_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
     gene            complement(92807..93337)
                     /gene="kefF"
                     /locus_tag="JN692_000078"
     CDS             complement(92807..93337)
                     /gene="kefF"
                     /locus_tag="JN692_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7262497.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(93385..94779)
                     /locus_tag="JN692_000079"
     CDS             complement(93385..94779)
                     /locus_tag="JN692_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262498.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(94838..95125)
                     /gene="fixX"
                     /locus_tag="JN692_000080"
     CDS             complement(94838..95125)
                     /gene="fixX"
                     /locus_tag="JN692_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7262499.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(95122..96408)
                     /locus_tag="JN692_000081"
     CDS             complement(95122..96408)
                     /locus_tag="JN692_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7262500.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEASAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTSENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(96459..97400)
                     /locus_tag="JN692_000082"
     CDS             complement(96459..97400)
                     /locus_tag="JN692_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7262501.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAC"
     gene            complement(97415..98185)
                     /locus_tag="JN692_000083"
     CDS             complement(97415..98185)
                     /locus_tag="JN692_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7262502.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            98600..100114
                     /gene="caiT"
                     /locus_tag="JN692_000084"
     CDS             98600..100114
                     /gene="caiT"
                     /locus_tag="JN692_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY7262503.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWMFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            100145..101287
                     /gene="caiA"
                     /locus_tag="JN692_000085"
     CDS             100145..101287
                     /gene="caiA"
                     /locus_tag="JN692_000085"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7262504.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            101416..102633
                     /gene="caiB"
                     /locus_tag="JN692_000086"
                     /pseudo
     CDS             101416..102633
                     /gene="caiB"
                     /locus_tag="JN692_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            102707..104260
                     /gene="caiC"
                     /locus_tag="JN692_000087"
     CDS             102707..104260
                     /gene="caiC"
                     /locus_tag="JN692_000087"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7262505.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDHPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            104369..105154
                     /gene="caiD"
                     /locus_tag="JN692_000088"
     CDS             104369..105154
                     /gene="caiD"
                     /locus_tag="JN692_000088"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7262506.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            105160..105750
                     /gene="caiE"
                     /locus_tag="JN692_000089"
     CDS             105160..105750
                     /gene="caiE"
                     /locus_tag="JN692_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7262507.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(105836..106231)
                     /gene="caiF"
                     /locus_tag="JN692_000090"
     CDS             complement(105836..106231)
                     /gene="caiF"
                     /locus_tag="JN692_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7262508.1"
                     /translation="MCEGYVEKLLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(106493..109714)
                     /gene="carB"
                     /locus_tag="JN692_000091"
     CDS             complement(106493..109714)
                     /gene="carB"
                     /locus_tag="JN692_000091"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7262509.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(109732..110880)
                     /gene="carA"
                     /locus_tag="JN692_000092"
     CDS             complement(109732..110880)
                     /gene="carA"
                     /locus_tag="JN692_000092"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7262510.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFLGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(111336..112157)
                     /gene="dapB"
                     /locus_tag="JN692_000093"
     CDS             complement(111336..112157)
                     /gene="dapB"
                     /locus_tag="JN692_000093"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7262511.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(112324..113238)
                     /gene="rihC"
                     /locus_tag="JN692_000094"
     CDS             complement(112324..113238)
                     /gene="rihC"
                     /locus_tag="JN692_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7262512.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(113304..114254)
                     /gene="ispH"
                     /locus_tag="JN692_000095"
                     /gene_synonym="lytB"
     CDS             complement(113304..114254)
                     /gene="ispH"
                     /locus_tag="JN692_000095"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7262513.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(114256..114705)
                     /gene="fkpB"
                     /locus_tag="JN692_000096"
     CDS             complement(114256..114705)
                     /gene="fkpB"
                     /locus_tag="JN692_000096"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7262514.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(114792..115286)
                     /gene="lspA"
                     /locus_tag="JN692_000097"
     CDS             complement(114792..115286)
                     /gene="lspA"
                     /locus_tag="JN692_000097"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7262515.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(115286..118102)
                     /gene="ileS"
                     /locus_tag="JN692_000098"
     CDS             complement(115286..118102)
                     /gene="ileS"
                     /locus_tag="JN692_000098"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7262516.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYVEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(118145..119086)
                     /gene="ribF"
                     /locus_tag="JN692_000099"
     CDS             complement(118145..119086)
                     /gene="ribF"
                     /locus_tag="JN692_000099"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7262517.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(119094..119312)
                     /locus_tag="JN692_000100"
     CDS             complement(119094..119312)
                     /locus_tag="JN692_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7262518.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            119415..119678
                     /gene="rpsT"
                     /locus_tag="JN692_000101"
     CDS             119415..119678
                     /gene="rpsT"
                     /locus_tag="JN692_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7262519.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        28786 a        33042 c        30356 g        27822 t
ORIGIN      
        1 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
       61 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
      121 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
      181 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
      241 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
      301 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
      361 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
      421 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
      481 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
      541 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
      601 gatatcttca acaatcagca cgtccttgcc acggatatcc tcatccagat ctttgaggat
      661 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
      721 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
      781 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
      841 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
      901 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
      961 caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat
     1021 aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct ataattaatt gctactaaaa
     1081 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
     1141 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     1201 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     1261 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     1321 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     1381 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     1441 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     1501 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     1561 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     1621 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     1681 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     1741 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     1801 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     1861 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     1921 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     1981 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     2041 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     2101 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     2161 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     2221 caccggtgag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     2281 tgacgaacac acctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     2341 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     2401 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     2461 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     2521 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     2581 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     2641 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     2701 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     2761 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     2821 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     2881 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     2941 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     3001 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     3061 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     3121 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     3181 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     3241 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     3301 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     3361 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     3421 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     3481 gccggatgat gtgaaataac cataatgccc ggagagatcc gggcatattt ccgaatacgg
     3541 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     3601 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     3661 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     3721 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     3781 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     3841 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     3901 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     3961 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     4021 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     4081 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     4141 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
     4201 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     4261 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     4321 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     4381 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     4441 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     4501 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     4561 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     4621 agaccagcca gccccatcgc cacctgtcgc ccggtcttgc catgttgatg tggatggaac
     4681 cagcaggtag cggcaggttg atcaacgttc aacctcaccg agcgcttgcc acctggcgga
     4741 ataattccct gtgggccgcc gtcgacgtca cccggtactt ccagcccgtg ccagtgtaac
     4801 gtcgtctctt ccgccagttg gttgtagata tcaaccgtta ccgctttgcc gcgctgtaat
     4861 ttcaccgccg gccccagcag attgccgtta tagccccagg tagttgcagt tttctcgcca
     4921 aaggtggact gacctgcgcc aatagttaac tgaatgcgat tacgggcatc ggtcgtgagc
     4981 aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga ctgcacggct ccacagtggc
     5041 aaggctgaag ccacacccag tgcgacggaa tatttcaaga aatcacgacg ttgcatagtt
     5101 atttccttat tcttaagcag gcgaaacaaa atcaaacgtt gagcatagtc cttcccctta
     5161 cgggaaggtc aagccgcaga cacaatttaa tcgacaataa tggtcgtcgc ctcgctcgca
     5221 gtgtgctaac gtttatcttc tttaagcccc tggtagaagc aatgaagacg tttttcagaa
     5281 cagtgttatt cggcagcctg atggccgttt gcgcaaacag ttacgcgctc agcgagtctg
     5341 aagccgaaga tatggccgat ttaacggcag tttttgtctt tctgaagaac gattgtggtt
     5401 accagaactt acctaacggg caaattcgtc gcgcactggt ctttttcgcc cagcaaaacc
     5461 agtgggatct cagtaattac gacaccttcg acatgaaagc tctcggtgaa gacagctacc
     5521 gcgatctcag cggcattggc attcccgtcg ctaaaaaatg caaagccctg gcccgcgatt
     5581 ccttaagcct gcttgcctac gtcaaataat ccctgatacc tttttgtaga aatattgacc
     5641 gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt tttttacggg tgttaacaaa
     5701 ggaggtatca acccatggcc gaaaaaaaac agtggcatga aacgctacac gaccagtttg
     5761 ggcagtactt tgcggtagat aacgttctgt atcatgaaaa gaccgatcac caggatctga
     5821 tcatttttga gaacgctgca tttggtcgcg taatggcgct ggatggcgta gtacaaacca
     5881 ccgagcgcga cgagtttatc tatcatgaga tgatgaccca tgttccgcta ctggcccacg
     5941 gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg tgccatgctg cgtgaagtaa
     6001 cccgacataa aaacgttgag tcaatcacga tggtggaaat cgatgcgggt gtcgtgtcgt
     6061 tctgccgtca gtatctgccc aaccataacg ccggtagcta cgacgatccg cgctttcaac
     6121 tagtgatcga cgatggcgtc aatttcgtta atcaaaccag ccagaccttt gatgtcatta
     6181 tctccgactg caccgatcct atcggtcccg gcgaaagcct gttcacctca gcattttatg
     6241 aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt cgcacaaaac ggcgtctgct
     6301 ttttacagca ggaagaagcc atcgacagcc atcgcaaact cagccattac ttcagcgacg
     6361 ttggctttta tcaggcggcg atcccgacct attacggcgg tatcatgact tttgcatggg
     6421 cgacagataa cgacgcctta cgccatctct caaccgaaat tattcaggcg cgttttctcg
     6481 cttctggcct gaaatgccgt tattacaatc cggcaatcca tacagcagct tttgccttac
     6541 ctcagtatct gcaagacgca ctggcttcac agccgtccta aggagaagat aagaaattga
     6601 aaaaactgaa actgcatggc tttaataatc tgaccaaaag tctgagtttt tgtatttacg
     6661 atatctgcta cgccaaaact gccgaagagc gcgacggtta tattgcttat atcgatgaac
     6721 tctataatgc caaccgtctg accgaaatcc tgtcagaaac ctgttccatt atcggggcca
     6781 atattcttaa catcgcccgc caggattacg aaccacaggg tgccagcgtc actattctgg
     6841 tgagtgaaga accggttgac ccgaaactca tcgacaaaac agaacacccc ggcccactgc
     6901 cagaaacggt ggttgctcat ctcgataaaa gtcatatttg cgtacatacc tacccggaaa
     6961 gtcatcctga aggcggttta tgtaccttcc gcgccgatat tgaagtctct acctgcggcg
     7021 tgatttctcc gctgaaggcg ctgaattacc tgatccacca gcttgagtcc gatatcgtaa
     7081 ccattgatta tcgcgtgcgc ggttttactc gcgacattaa cggtatgaag cactttatcg
     7141 accatgagat taattcgatt cagaacttta tgtctgacga tatgaaggcg ctgtatgaca
     7201 tggtggatgt aaacgtctat caggaaaata tcttccatac caagatgttg cttaaagagt
     7261 tcgaccttaa gcactacatg ttccacacca aaccggaaga tttaaccgac agcgagcgcc
     7321 aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat ttattacggg cgcaatatgc
     7381 cagctgttta acggctctgg cggagctccc aggctccgcc agatttattt acttctgctg
     7441 cacgaaattg cggtaagccg ccacgacctg cagaaaatcc tcaacgccgc atagcgacag
     7501 gctttcttcg tcgtagtagt tcatcccctc ttccatttca tcgccagcga attccagttg
     7561 attggcgcga accatcacct cttcagcgtc catccacagg gtgtattcat gccctgcccg
     7621 ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct tgttccactt catcaagcaa
     7681 ggccaggttt tctttcacct cttcattaaa ccagtgcccg accacttcat gccccatgga
     7741 catacgcacc tttaccactc cggtaatatc gcgcagaaat tcgtaatcca tagtgtgttc
     7801 ctctgctccc ggcaatgcgc cgaaatcgca tcgcgccatt gatgtaatta tcgcagcctt
     7861 accggagaaa aacagcggaa cggcgatggt ttagaaataa aaaatgcccg ggaatctcat
     7921 tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     7981 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     8041 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     8101 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     8161 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     8221 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     8281 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     8341 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     8401 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     8461 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     8521 gattggctct ttaatatccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     8581 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     8641 cagggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     8701 gttcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     8761 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     8821 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     8881 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     8941 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     9001 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     9061 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     9121 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     9181 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     9241 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     9301 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     9361 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     9421 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     9481 aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     9541 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     9601 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     9661 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     9721 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     9781 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     9841 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     9901 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     9961 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    10021 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    10081 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    10141 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    10201 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    10261 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    10321 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    10381 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    10441 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    10501 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    10561 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    10621 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    10681 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    10741 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    10801 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    10861 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    10921 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    10981 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    11041 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    11101 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    11161 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    11221 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    11281 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    11341 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    11401 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    11461 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    11521 aaagctgtac cggtaaaaca gacagtcacg gcccccgtca taccatccaa taccgtttta
    11581 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    11641 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    11701 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    11761 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    11821 gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    11881 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    11941 gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat ggcatggcgg
    12001 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    12061 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    12121 agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    12181 acacgagata cccaacgtca ggcatcggca cagcggttta atgaagctga acactatggg
    12241 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca acaaaaggac
    12301 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    12361 atgcagacta acccgcagat ccagcagcga agggaggcgg cgcgccagcg tattcagtcc
    12421 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    12481 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    12541 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    12601 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    12661 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    12721 gcagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    12781 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    12841 attacttctt cttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    12901 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcgtg gatggtcagt gcgatgtctt
    12961 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttgg
    13021 taccaacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    13081 taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    13141 tttcatagct gatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    13201 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaaatagtgt ttcttaccgg
    13261 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc atcggctgac
    13321 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    13381 ggatgaaacc acggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    13441 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gctggtgctt
    13501 ttttgccttc catcgtcaca taaataccgt cttctttcgc ttcaacggcg gtaactttgg
    13561 tttccagcat caggttgaat ttcttgctga tacgcttggt gaagacttta acgatgtctt
    13621 tgtcagctgc cgggataacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    13681 cgtggtaaac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    13741 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    13801 gaataaacgg cagttggatt gggcgagaac ccgctgcaat gatcgcattg tcgaagttga
    13861 tcacggtttt gccgttctca ccttcaactt ccagggtgtt agccccggtg aatttaccca
    13921 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    13981 gattgatcac tttctctttc caggtacgaa tcttgtcaat atcggttttc ggttcgccga
    14041 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    14101 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccaagggtg ttgtaacgtt
    14161 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    14221 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    14281 atttatctcc ggcggtcata cccgtcgtct ttcaggccgc agcggcgtta gcttcacgcc
    14341 ctcaccccag ccacttactt atgtaagctc ctggggattc tggcgcttgc taccttgctg
    14401 cacccagaaa tccatagggt atgtgaagta ataccctaac caccaccggg tcgttctatc
    14461 cgtcggtatt ttacaaaatt gttaacaatt tttaaacaac aaacggcaac cgatttgtct
    14521 attcgctaat aacctcaata cattcatgag attaccagaa aaaagccggc cgttgggccg
    14581 gctcttttac ttacatcacc agacggcgaa tgtcagacag cgtgttgtta atgatggtaa
    14641 tgaaacgggc accatcagca ccgtcgatca cgcggtggtc gaaggagaga gaaatcggca
    14701 gcatcagacg cggcacgaac tctttaccat tccacaccgg ctccatcgcg gacttggaaa
    14761 cgccgaggat agccacttcc ggcgcgttca caatcggcgc gaagtgggta gtacccaggc
    14821 cgccgatgct ggagatggtg aagcaaccgc cctgcatttc gcccgcagtc agcttaccgt
    14881 cacgcgcttt cttagaaata gtcatcagct cgcgagacag ctcgatgatg cctttcttgt
    14941 tgacgtcttt gaataccgga acaaccagac cgttcggggt atccaccgcc acaccgatgt
    15001 tgatgtattt cttcagggtc agacgctgac cgtcttccga cagcgaacta ttgaagcgag
    15061 gcatctgctc aagagctgca gcaacggctt tcatgatgaa gacaaccggg gtgatcttca
    15121 catccagctt acgtttcgcc gcttcttcgt tctgctgttt acggaacgct tccagctcgg
    15181 tgatatcggt tttgtcgaag tgggtaacat gcgggatcat cacccagtta cggctcaggt
    15241 tagcaccaga aattttctgg atacggccca gttccacttc ttcgatttcg ccaaacttgc
    15301 tgaagtccac cttcggccac ggcagcatgc cagggatacc accgccagtc gccgccggag
    15361 ctgcttctgc acgtttgata gcttctttca cgtaagcctg aacgtcttcg cgcaggatac
    15421 gacctttacg gccagtgccc ttcactttcg ccaggttaac gccaaactcg cgtgccagac
    15481 ggcggatcag cggagtcgcg tgaacgtaag cgtcgttttc agcaaattca gatttgcctt
    15541 ccgctttcgc agccggagct gctgccggag cttcagcttt tgctgccgga gccggcgctg
    15601 ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg aagatcataa
    15661 tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc acgacgcctg
    15721 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc agtgactgtt
    15781 cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact tcgtcaccgc
    15841 cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc gctgccggag
    15901 ccgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact tcgaagacca
    15961 taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct ttcacggtac
    16021 ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg atcagcgact
    16081 gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca acttcgtcgc
    16141 tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct gctgccggag
    16201 ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg tcggcggaat
    16261 cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact ttgatctctt
    16321 taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct tctacggtga
    16381 tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg gtgatttcaa
    16441 cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt ttacctctta
    16501 cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg cgtcagcaac
    16561 cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa ccacgacata
    16621 agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac gaccgaagcc
    16681 atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct gctcagcgaa
    16741 cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct gagcgatata
    16801 cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat cctgaccatc
    16861 acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc cgtaatcttt
    16921 cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc ccagcagctg
    16981 aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac ggataccttc
    17041 ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag tgatgtagta
    17101 gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca taatgacagc
    17161 aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca gcgactgaat
    17221 gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac gaccggaagt
    17281 accgccgatc aggaagccac gcgcttgctg gtcgccagcc gcccagcaca gatcgccgat
    17341 acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca gattgttcgt
    17401 gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt tgatcccttc
    17461 ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc ggtcctgcgg
    17521 ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac cttccatacc
    17581 gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg acttgttctt
    17641 cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagatctctt tgctctgctc
    17701 ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg tgaagttcgg
    17761 ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat aggtatgctc
    17821 ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat cagacaccgg
    17881 cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct taacctggtg
    17941 cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg tatgagcaag
    18001 gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt agattttctt
    18061 cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt ctgcaaccag
    18121 tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac catctttcga
    18181 tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca gtttaccgct
    18241 ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga tcacgttcca
    18301 gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac cggtgaccgg
    18361 gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca gtttttcacg
    18421 ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt cgccgaggaa
    18481 cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat atttcaggaa
    18541 tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg ggaactgcca
    18601 gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt gaacttcctg
    18661 acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac gggcgtaaac
    18721 gcccggggag atgtggccct ggaagtaaac caagtcgccg ccatcctgct cgttgcgtgc
    18781 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
    18841 gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca
    18901 gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
    18961 ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta cgtttacacc
    19021 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
    19081 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
    19141 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
    19201 gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta cgtttagttg
    19261 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
    19321 actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc tcttcacggc
    19381 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
    19441 cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
    19501 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
    19561 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
    19621 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
    19681 gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta ctacgcagcg
    19741 cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag tcatactgtg
    19801 attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc cataggctgc
    19861 tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct tcgagacgtt
    19921 gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt tcgcgttccg
    19981 gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc agttgctgct
    20041 caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt tcctgtctta
    20101 agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct aaagtaacaa
    20161 agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg cacattttaa
    20221 caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga ctttacgtag
    20281 gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg attcacttac
    20341 caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca tattgagagg
    20401 ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata
    20461 aagcgggtgc attcgctgcc gtataccatt attcttgatc tgacggaagt ctttttgtaa
    20521 caattcaaac ttccttgatg taaataaatc aatacaacaa acggaattgc aaacttacac
    20581 acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga tggaaggtca
    20641 acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct
    20701 gggtggcgcg atagggacag ggctattcct gggtagcgcc tccgtaatac agtccgcagg
    20761 gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca
    20821 gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa
    20881 atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt
    20941 agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt atccggaaat
    21001 cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa
    21061 cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta ttgcggtagt
    21121 agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc cgcaggcgag
    21181 cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg gactggtgat
    21241 gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc
    21301 agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta tctaccgcat
    21361 cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt
    21421 taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc
    21481 gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata
    21541 ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa aagcgctggc
    21601 gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc tggttacagc
    21661 attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt taatggcact
    21721 ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata tgaagttccg
    21781 tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg
    21841 taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg
    21901 aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct
    21961 gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg
    22021 tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt agcgcaatca
    22081 ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca
    22141 ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt aatacctgat
    22201 attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat
    22261 gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa
    22321 gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt
    22381 attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag gttcccccca
    22441 caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt
    22501 gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga
    22561 gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag
    22621 catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca
    22681 ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca
    22741 cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc
    22801 gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct
    22861 cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc
    22921 acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc
    22981 acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg cccgcgtaac
    23041 cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc cttcaagctc
    23101 aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga ctcccgcatg
    23161 ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca
    23221 gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat
    23281 agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat
    23341 attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt aatgtggtga
    23401 gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat
    23461 atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc
    23521 tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga cgagctgctg
    23581 gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc
    23641 ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taacggaaaa ttctcgctct
    23701 catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg ggttgaagag
    23761 gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat
    23821 gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg
    23881 ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc
    23941 cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg caaaacctta
    24001 cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt
    24061 ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg
    24121 cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga agtcgaagta
    24181 gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat catgctggat
    24241 aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg
    24301 gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac
    24361 tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt
    24421 cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac
    24481 tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg
    24541 aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta
    24601 gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga
    24661 actgatggtg gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca
    24721 aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac
    24781 cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa
    24841 tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt
    24901 ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga cgccaggttg
    24961 ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa gtgacagcgc
    25021 attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat
    25081 gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc tggatgccag
    25141 cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc tggacgcatt
    25201 gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg
    25261 tcacgccagt cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa
    25321 agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca
    25381 tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc
    25441 tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa aagtgctggg
    25501 aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc
    25561 aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt
    25621 attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg gaatgcagcc
    25681 gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac
    25741 cggccctaca ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac
    25801 cgctgacatt aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa
    25861 ccagacgcaa atccatccgc gtgccgggct cacctttcag ggcgttttgc gtgcgttatt
    25921 gcgccaggat cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cggcagaaat
    25981 tgccattaaa gccgcgcaaa ccggtcacct ggtgttgtcc acgctacaca ccaattccac
    26041 cactgaaacg ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc
    26101 gcttacgctg gtaatagccc agcgtctggt acgtaaactt tgcccacatt gtcgccgaca
    26161 gcaaggggag cccatccata ttccagacac tgtatggccg tcgccgctgc cccactggca
    26221 ggcacccggt tgtgtacatt gctaccacgg tttttatggt cgtaccgcct tgtttgaagt
    26281 cctgcccatt acaccagcca tacgccagct tatttccgct aatactgaag ttgaatcgct
    26341 ggaaacgcac gcccgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt
    26401 ggagcaaggc ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcaa
    26461 gtaagcaact ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc
    26521 tatgggcaga gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac
    26581 taagcctgaa gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag
    26641 tcattcatca actggcgacg ctacttaaag ccgggttaac gctttctgaa gggctggctc
    26701 tgctggcgga acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg
    26761 atctcgaaca gggcattgct ttttccaatg ccttattacc ctggtcagag gcatttccgc
    26821 cgctctatca ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct
    26881 ttgaactggc gcgtcagcaa aaatcccagc gtcagttgac cgacaaagtg aaatcagcgt
    26941 tgcattatcc catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt
    27001 ttgttctgcc ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa
    27061 cgcaggggat catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt
    27121 tcggctttct gctggcgata gccaataagt tgctgatgcg ccgcccgacc tggcttatcg
    27181 cgcggcagaa attgctgtta cgcatcccga ttatgggttc actgatgagg ggacaaaaac
    27241 tcacgcagat ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg
    27301 gcgtagagag cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa
    27361 tccagcacga tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt
    27421 ttagcccgct ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca
    27481 tgttagacaa cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg
    27541 cagccttact ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg
    27601 tggcaatgta tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct
    27661 ggcgtgttgc tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg
    27721 ttccagcaca cgaataaacg tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac
    27781 gtatgtacaa gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc
    27841 tcgcagcggc agcttaacgg ttttaccttc tgctgcgcgg tattccgcaa cgccgccaac
    27901 gtgacgtttc atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt
    27961 ctcctcaacg atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac
    28021 gaaatcggca ccgccgccaa aggctttcgc cacatcgccc ggcgtggtac agccaccgtc
    28081 gctgacgatc attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag
    28141 ctgcggataa ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc
    28201 aactttaacg atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac
    28261 gttgccagca caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac
    28321 gaagtgttcg gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag
    28381 gtcgagaatc tgtttagttt tttcgaaatc cgcatcagac gtgccggtag aaaccatcac
    28441 atgtttcagc acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaaaa
    28501 gtgtttatgc acagcagtca gaatatcaaa agaagccagc gcagaggcca tagaaaatgt
    28561 gcctacggtg tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg
    28621 tttgaaggtg aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt
    28681 agggcggatg agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg
    28741 attcctgggg ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca
    28801 ctaatcaatg cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt
    28861 tacctggtaa atagtgagcg aacgtttcat caatttcact ttcgcgcgct catttttaac
    28921 gttttgataa tcatgatgaa ttcccgggaa tataaattat ggggtatata gttgccttaa
    28981 cgggaggcat tggcagtggt aagagtaccg ttgccaatgc gtttgctgat ctcggaatta
    29041 acgtcattga tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac
    29101 atgccattgc tgatcacttt ggcgaaaaca tgattgctgc tgatggaaca ttgcagcgcc
    29161 gggccttgcg cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc
    29221 tgcatccgct gattcagcaa gagacgcaac accagatcca gcaagcaact tccccctatg
    29281 tactgtgggt tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc
    29341 tggtggtgga tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa
    29401 ctcgcgaaca tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg
    29461 tggcagatga cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc
    29521 gcctgcacgc acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat
    29581 gcagacccag gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat
    29641 tgagtttttg attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct
    29701 gcatttcttc cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac
    29761 tgagctgttg aaagaactta accggcagca acgtaaactc cagacctgga ttggcgtgcc
    29821 tggcgtggac cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt
    29881 attaatttcc gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt
    29941 gcgtcagcga ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat
    30001 ttggctgcat ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct
    30061 gaacccgctc acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt
    30121 ccgtaaacaa accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct
    30181 gcgcctgaat ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg
    30241 ttttgccatt cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga
    30301 tttcgaactg gcctgttgct aaggagtaaa aatgtcagaa actattacgg tgaattgccc
    30361 aacctgcggg aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa
    30421 acgttgtcag ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag
    30481 tggcgatctt tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga
    30541 tgcctcacgt aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt
    30601 cggatgcggc gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt
    30661 cattggctgg cggaaaatca tcggcattaa gaccgaccag cgacatccac tcaccgggtt
    30721 gcccttcttt accccacggc accccttccc aactttcgac cagccagaac cacagagtta
    30781 tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa
    30841 tcccgacctc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa
    30901 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgtgtta
    30961 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    31021 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta
    31081 ttgcaggcga ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt
    31141 acgtcctact ttgcgtttac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc
    31201 atcctgatgg ctaagctgct gcatttgtgc taaacgctcg gcttccatac gacgctgttg
    31261 ttccagctcc tcaacctctt caggcatacg tacctgaact ttgctcagcg tactgataac
    31321 ttcatatttc aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    31381 ctgcttcgga tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat
    31441 cgctgccagg tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa
    31501 gtgacgcatc atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg
    31561 cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    31621 ggcaattggt aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc
    31681 ccacatttct tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc
    31741 ttcacgaatg ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    31801 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    31861 aatgtcgaag ttacgacttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca
    31921 cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    31981 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    32041 agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg
    32101 acgctcggta ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg
    32161 ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc
    32221 tgcctgccag ctacctccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat
    32281 agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt
    32341 ggcgttcagg acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga
    32401 tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat
    32461 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagactaggt ccggcagatc
    32521 tttacgaatc attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct
    32581 aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg
    32641 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    32701 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgtcggccct gcatggtacg
    32761 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg
    32821 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    32881 cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat
    32941 cagcaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg
    33001 gccttcgccc tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat
    33061 tttattcacg cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg
    33121 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    33181 acgctgtaca cgttcttcag ggctgaacgc catgttgtcg cgcaggtagt caaagccgta
    33241 ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg
    33301 catgcccggc aggttgatac caacagtcag gccaaggaat tcaaacagcg gacggttgtt
    33361 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt
    33421 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    33481 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    33541 cataccaaag acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt
    33601 ttccagcact tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc
    33661 ttcatcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    33721 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag
    33781 cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt
    33841 gttgttaggt gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg
    33901 gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    33961 acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    34021 cctgaagagg caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa
    34081 gatgacggat taccgtgcga atagcatgtt gatgccagta atcaacggaa tagttcgaat
    34141 tcggccggcg cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    34201 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    34261 caggcaaacc taaactcgcc gcgaccatcc ctaataagag atgcggccag aagtagcgtt
    34321 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgaatt
    34381 caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag
    34441 ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgttttta tagctcttat
    34501 aaaattcaaa aaaaaacgac tggttcacct agccggagag agtgccagat ttgccagtcg
    34561 aattttatac gacagtataa atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc
    34621 aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg tttcgccagg
    34681 acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt aaatgcacca
    34741 ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca
    34801 aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg
    34861 gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc gatatcacgc
    34921 atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc ggagaagttc
    34981 atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat ggtgaaatcc
    35041 agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc gacacgaaca
    35101 gtctctttga tgcgaacaaa ttttttggcg cagttcaact cgtcgatacc ggcgtcaagc
    35161 agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg cgcgttaact
    35221 tcgataacaa tgttatcgat gcccaggccc gcgagagcag cattgaggtg ctctacggtt
    35281 gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc acgcacagat
    35341 ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata gatgaccccg
    35401 gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg taaaccgaca
    35461 cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa
    35521 attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca caaagagcct
    35581 cgaaacccaa attccagtca attcttaatc agcttgctta cgtaggaatg ctgggatatc
    35641 cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcactactt tagcaaccgg
    35701 cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca tcactggctg
    35761 ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt
    35821 cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag taccgataac
    35881 cacagtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt cgaactcatc
    35941 cagacgcagg tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc
    36001 gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg cacggtcttc
    36061 accgctcgcc acgccagaac ccatcattgc gtagcccatt tcagacatta cggtgcgtac
    36121 gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga taccttgcac
    36181 agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc
    36241 cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat gcttggacag
    36301 ttcagtgatc ccctgctcgg cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt
    36361 agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga cgactggtgc
    36421 tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt ctgcaccttc
    36481 cagcgccgca cgcaatgcat cgcgatcctc atcagccgca ttgcggccaa cttctggatt
    36541 agcgccagcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc
    36601 tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac cttcaatacg
    36661 ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga tgactttaat
    36721 caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat ttgtgcctgt
    36781 cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc gcagccaact
    36841 attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta cttcagcttc
    36901 accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg aataatacgg
    36961 ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac gcacttgcgt
    37021 atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc caccggttaa
    37081 tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt cctgcaactg
    37141 caatatctct tcgttgacca ggttgagcaa ctcggtatag cgcggctcga tcacctctgc
    37201 cagggtctga cgttgcagac tccgcggcgg acgaccgcct acgctcggca cttccacgct
    37261 ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc
    37321 gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat tgcctgcata
    37381 aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat ccattgtacc
    37441 accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca ataccgaata
    37501 acttgatgcc agtccggcaa atatcagttg gtcaactttc agcccacaac gttcaaccgc
    37561 tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat
    37621 ccgcacaccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa tcgcatactc
    37681 ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg
    37741 gacgacattt tccacatctt cttgcgtcac ttcttcttca gaaataggca ccataccaat
    37801 ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag agatctgaca
    37861 atctgccatc aattctgcct ggtcaatggc gcgttgtacg cacttgacca cggattcgag
    37921 gtcgttcacc ccgcctttat ccataccacg tgacgggcag ctgcccacgc caatgatatt
    37981 gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg taccaatttc
    38041 cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga
    38101 ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc cgctccagag
    38161 tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa
    38221 accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg gccaagattg
    38281 agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc
    38341 ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc ctgcaacact
    38401 tcattagcgc tgccttccgg gccatacagc attggaagca cctgcttgct ggtgcgatct
    38461 ggcggcacgc tgaaggtgtt tccttccgcg tctaccatgt gttgatcatt ccaccgcgca
    38521 atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt tctgacgctc
    38581 acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt gacatcctgg
    38641 gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc gtcattacgc
    38701 gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg cgcatcttcc
    38761 atccagctca acacgaccca gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa
    38821 aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc ttcgctgttt
    38881 cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc gtactaccaa
    38941 ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt ggctggtcat
    39001 acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca gcataacgtc
    39061 aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta atgcctgcaa
    39121 attggcctct tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct catcagagag
    39181 atacttcgcc tcataatcat agaaggttcc ggacggttga atacgtattg acggtaaaat
    39241 ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt caatcaatac
    39301 ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt ctgctactac
    39361 ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg gcaaacccag
    39421 agcagaaatt tctgctaact gcttatcgct caggcctttt tcaaactctg cgcgggttaa
    39481 tgctacccac ggcgcgaccg gtaaaccggc accttgccac agaagtttgc tgcgtagttt
    39541 atctattgaa agcgcagatg ccatcactcc gctgccggta taaggcaagc ccatcagctc
    39601 gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga taaacacttt
    39661 ctgaaaaccc atcgacttca gttgcgtcac gtcgacttct ttcgggtcga caggatacgc
    39721 atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca gagaaacttc
    39781 ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt tgttcttcct
    39841 ccggagtttg cggcttcagt ttgatttcag ctaaagaacg ggcaattttt ccgatattac
    39901 cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc atctcggcta
    39961 cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga attgtgcgac
    40021 acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac acttccagca
    40081 tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac
    40141 gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag cctgcgcgcg
    40201 ccgctttaat ggtggcgtca acttccgtcg ggtggtggcc gtagtcatcg accagcattg
    40261 ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa tcaaaacggc
    40321 gaccagtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca attccctctt
    40381 ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacggcct ggcgcattca
    40441 gggtgacgcg catcggctct ttgtcctgac gcagcagcgt aaagtgcccc tgcgggccaa
    40501 tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccgtaa gttgtggtct
    40561 gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac atcaccgcac
    40621 gaccgtaaaa aggcaggttg tgcagaaaat taataaaggt ctgttttaaa ttctcaaagt
    40681 cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc accatcggtt
    40741 gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac cgaccatgcc
    40801 ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag gttgggtcga
    40861 gccccgcttc tgcgtagatg ctggaaacca tcgcggtagt tgtcgttttg ccgtgcgttc
    40921 ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg gcacgacgga
    40981 tcaccggaat acgcgcttca tgagctgcga caatttccgg gttgtcggca gaaatggcgc
    41041 tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg aaataaatcg
    41101 tcgcacccag attcagtaac tgctgcgtga ccggatttgg cgctaaatcg gaaccactga
    41161 tctgataacc ttcattggcc agaacttcgg caataccgcc cataccggca ccaccaatgc
    41221 cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc agttttgcca
    41281 attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat gcaaaaggca
    41341 tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct cggtggcatc
    41401 cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc gcgaccaccc
    41461 ggccagggtg ttggcgacag catccacgct aagctgtggc tgctcgataa ttttggctgc
    41521 gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat gttgaaacgg
    41581 cacaaacaac gccggtagtc ctgccgcggc gatttcactc acagttaaag caccggagcg
    41641 gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa attcagtcac
    41701 tttatgctgc ggttgccccg cttcggcata cgcctgttca acggattgtt gcgaaccttt
    41761 gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct gcggcattgt
    41821 ctggttaaga atgcgtgcgc cctgggaacc acccactacc aacacacgaa ccggaccttc
    41881 acgtccagcc aaacgttgct gcggcaacgg cagcgccagc acatcggtac gcaccgggtt
    41941 acccaccact tccgcattag ggaaagcgcc tggaaacgcc tgcatcactt tggtagcaat
    42001 cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg
    42061 aatgcctaac gaccacgcgg ccagaccacc tggacctgat acatagccgc ccataccgag
    42121 taccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg cgttgaagat
    42181 acgcagcggc gcagctatca gtgcttttat accttttcca cgcaaaccag agatacgaat
    42241 gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt cggcagtccc
    42301 cagccagcga acttgccaac cctgagccat tagatagtgc gcaaccgcca gtcccgggaa
    42361 tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac cactcatcgt
    42421 gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat acgcaacagc
    42481 atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat tagcggcaat
    42541 gtcagacctt tggtcggtaa catccccgcc gccgcgccta cgttaaccag cgcctggaag
    42601 ctaaaccaga tgccaataga acaggcgaga aaaccggaaa aacggtggtc aatttctaat
    42661 gctttgcggc caatcgacat cgcacgaaaa gcgacgaaga ataccattaa aagtgctagt
    42721 accacaccga cataacctaa ttcttcgccg ataatggcga aaataaagtc agtgtgcgct
    42781 tccggcagat actccagttt ttgtaccgaa ttacctaaac cttgccccca aagttcgccg
    42841 cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg atcttcccac
    42901 gggttccaga atgcggtaac acggcggata cggtacggtt cggcgaggat cagcaacaca
    42961 accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc tcccgccagg
    43021 aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc tggctgtgcc
    43081 agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta
    43141 ttacgtactt cgtcgccttt acgcaccaga tagttggcga tatagcaaaa cagcgacagt
    43201 tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg cgatgcccct
    43261 ttaacagcgc tacccactac cagcacgatc atcagcagga tgatagatcc gagcagcatc
    43321 gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat cgccagaata
    43381 aacgccaaaa tcagatagac accatcacgc ttcgcgaaga agaacggatc gttggttaag
    43441 cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc cgcgaggccg
    43501 aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc
    43561 gagcccatca cccagccctt tagcgccgtg gagatccaga ccaggatact gaatcctggc
    43621 aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc ttcgccagac
    43681 gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg gcacaggccg
    43741 gggagagcag aaccatatcg cccggctgaa cacgcggagc cagcaagcgc atagcctgtt
    43801 ccatagtttc ggtttgttct gccacttccg ggcgtagcgc cgccagctgc gcgccgtcac
    43861 gaccgaaaca atacagacgt acgttatcgc cattcaggta acgcgccagt gggctaaagt
    43921 ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacg tgcaggccat
    43981 tcagcgccgc ttccgtactg ccgacgttgg tcgcttttga atcgttaatc cagcgcacac
    44041 cgttatgctc cagcaccact tcaaagcgat gcggcagacc ggtgaaggtg gttaacgctt
    44101 tcaggctgct ggcgcgcggt aaccctgcgg catctgccag cgccagcgcc gccagcgcat
    44161 tggtgtagtt atgctgcccg gaaagtttca tctctttcac attcagcact ttctcgcctt
    44221 taacccgcag ccaggtttcg ccctgttgat gattcaggtg atagtcaccc atgttgacgc
    44281 caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca tcatcagcat
    44341 taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta
    44401 aaccaaacgg gtagcgatcc atatgatctt cagtcacgtt cagaatggtc gctgctaccg
    44461 cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg tacagttcac
    44521 actcatcatc cagtagcatc aacgcaggca ggccaatatt gccacccaca ccaacgttaa
    44581 cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg ccattagaac
    44641 cggtaatcgc cacaatcggt gcttgtgctt cgcgacaaaa cagctcgata tcgccaacga
    44701 tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca ataccgggac
    44761 tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc gtgtggcgtt
    44821 ctacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg
    44881 gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg cccaggccga
    44941 taatgacaac atttttaccc tggtaatcag ccatgattaa cgtaccttca gcgttgccag
    45001 accaatcagg accagcatca gcgaaataat ccagaaacgc acaatgacgc gcggttccgg
    45061 ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg
    45121 cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga acacgccccc
    45181 cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta acgcaccacc
    45241 taacgccagc gaaccgacat cgcccataaa gacctgcgcc ggataggtgt taaaccacag
    45301 gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc cggcgtgtcg
    45361 cagatacggt atatgtaaat agctggcaaa gttcatgttg ccggtcgccc acgccaccag
    45421 cgcaaaacca ccggcgacaa atacggtcgg cataattgcc agaccgtcaa gaccatcggt
    45481 caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa tgtagaacag
    45541 ccccagttgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc
    45601 tttgccggca aggtacaaag cgaaagcaac acccagcgca atgaccgaca tccagaaata
    45661 tttccaacga gcgatcaacc ctttggtgtc tttacgcacc actttgcgat aatcatcaac
    45721 aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt acggattgga
    45781 cgggtaagcc cacagcagca cggagatcac aatcgctgtc aggatcataa tcccgcccat
    45841 ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca ctacctgacc
    45901 aaaggaaagt ttttgcaaat gagcaatcat gcgcgggccc atccacaatg agatgaacag
    45961 cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga
    46021 ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc ctgtaaagcg
    46081 cgtactacct cttccatggc agcactacgt gaacccttaa ctaaaatcgt aattacctgt
    46141 tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc aaaatgttcg
    46201 ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccatgcttaa cacgcggtca
    46261 ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct ttcagcgccc
    46321 agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc cagtacctga
    46381 acagctgcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag caactggttt
    46441 tctgccagtt ggatggggaa cagacggcca ggaacagctt tcagatttgc cagccccgct
    46501 ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc cagcgcattc
    46561 gcaatattgt gacgccccgg caacggcagc agaacatcga cgctaccggt tggggtttgt
    46621 agggtaaatt ccataccgtg cgaggtcaca tggatattgg tggcggtgaa atcgctgttg
    46681 gcggcattgg gtgagaaacg ccacactttg cgtgagccaa ttacgctctg ccagttcagc
    46741 cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc gctaaagatt
    46801 tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg cgccgctgcc
    46861 aggttgttga ccagcgccgc ttccgggcga gtcagactca cagtccaggc aatctcgccc
    46921 tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa gcgcaacagc
    46981 gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt gttgccgcac
    47041 tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga ccccgtcaga
    47101 gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc aaacgccaga
    47161 cgcgtatcct tgacgattaa ctgcggcagg tcgatgtcca gcggacggct aaccagtagt
    47221 gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcgt gggcatcaaa acgttcacct
    47281 ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt tacagcatca
    47341 agggtgatat ctgcaccttg cagttcaccg ttgagaatat cggtaagttg gctaagggtt
    47401 acgctaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg gagtagtcca
    47461 gacgctgatt gccaacaatc tggtaatcct catggccttt gcccgcgacc agtaccacat
    47521 cattctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct tccatcactt
    47581 tggcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca cgcggttctt
    47641 cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc
    47701 ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagacg caccacagct
    47761 tgcccgcaca gtgcaggcgc gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg
    47821 cgtaatccac caccaccgtc ggtttacctg gcgcagtgaa cacttccata cgtccgcaaa
    47881 ccggttgcag acgcgcggcg gttttcagca gatcagccag tggatagccc agcgccagca
    47941 gtgttgccag cgcgagcaac aggttgctga cgttaaaagc gcccatcaga tggctttcaa
    48001 tttcgccatc gccccagctt gaactaaagc gaatggtcgc gccgctgtcg tgatagttca
    48061 cttcggtcgc tttcaaccag cgtccgtgac agttcggatt gatatgatct tccattgata
    48121 ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg tcggcgttaa
    48181 taatcgcctg accgcaatga tgttcagaat aaagcagcca tttcgcagct tcgtagtgtt
    48241 ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa
    48301 atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa
    48361 aagtcgcgcc ctgatccacc agccccgcca gctcatgctg aacatcgact gccgaaccgg
    48421 tagtattttc tgtcgggatc actttcccca gcagaccgtt accaacggtg cccattaccg
    48481 cgctggtttc gccaagcagt tggctccact gcgccagaag ctgggtcgtc gtggttttgc
    48541 cgttggtgcc cgttacgcct acgagacgta aattgtcaga gggttcatgg taaaagcggc
    48601 ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt acgccgtgca
    48661 tttcacggat ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg gcagccacac
    48721 cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta
    48781 caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg
    48841 aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc
    48901 ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg
    48961 atgttcatgg tacgcaatac gccgcccatg atggtaccaa agaccggcgc ggaaacggcg
    49021 ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc
    49081 tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc
    49141 ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc
    49201 ttcacgccgc cgccgcctgg tagcgccacg ctttccatca tatgcaccac ggtgcgaaca
    49261 atggattccg ggaagacacg ttcaccggga accggggggt caactttggt aatcgacagt
    49321 gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt
    49381 accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt
    49441 tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca
    49501 aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag cttggaaaca
    49561 ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg
    49621 tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga gttttcccgc
    49681 accacgccac gttgcaacgc ggtcatcacc accatcggtt taaccgttga gcccggttca
    49741 aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccgct cagattgtta
    49801 gggttgtatg acgggctgtt agccatcgcc agcacttcac ctgtgttgac atccaccagc
    49861 acggcgctac cagattcagc cttgttaaag gccaccgcgt tgttcagttc gcgataaacc
    49921 agcgcctgca ggcgttcatc aatactcagc gccaggttgt gcgctgcctg gctgtcagta
    49981 gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc
    50041 tgcccggtaa gccatttatc gaaactcttc tcaacgccct caatcccttg actatcgacg
    50101 ttagtaaagc cgatgaggtg agcagtcact tcgccggacg gatagtaacg gcgagactct
    50161 tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagggttt
    50221 acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg ggcagaaagc
    50281 tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata
    50341 ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc
    50401 gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg
    50461 cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac
    50521 gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa caacgcaaaa
    50581 cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcaccgct
    50641 gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca
    50701 acatgctgca tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc
    50761 gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc
    50821 agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc
    50881 gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg
    50941 tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg
    51001 cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata cgcagaactg
    51061 aactacgggc acgagggttc tcagccacct cttcttcgcc cggcattaac ttgcctagtg
    51121 ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg
    51181 gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg
    51241 agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta
    51301 gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac
    51361 gggtcgcggg atgtttaaac ttatctttca ccggcgttgc agcagccacg acttccgcca
    51421 gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc
    51481 gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg gcttcttctg
    51541 cggtttgtag ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg
    51601 gaccatcgcg cataaaggaa aagccgcgtt cagcatcatc aagttgcggt gaagagacgc
    51661 caagatcgag gagaatgccg tcgatcttgc caataagatc gcgctcggca acgtattcgc
    51721 ccagcgcgga gaaaggtccg tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa
    51781 cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg
    51841 agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag
    51901 gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt
    51961 ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc
    52021 tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac
    52081 ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact
    52141 ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt
    52201 aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa tttttgctcg
    52261 ataatttccc attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg
    52321 caaaccattt gaccggcagc gttctcaagc agctgttccc gataacgggt aggcactgat
    52381 aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctcaccc
    52441 cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc gtaggaaaag
    52501 cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc
    52561 agcgttgttt attttacgtc caacgtgcga agcaaattaa cgttatgaat agctgtaaga
    52621 aaaaatccct catcgtcatc actgttttaa aatgactcac tattttccac agcaaatata
    52681 aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtcct
    52741 tgttacgtct gtgtggcgct tgatgccagc gggtttgcgc aaaagcaaag aaaagtgttt
    52801 gttaaatcgg caattcatcg ccgcatgtaa caaaataatg caaaaacgcg ctgtcgatat
    52861 cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt aaaaccattt
    52921 atcaataata gtcaggaata gtcttattta ctttaaggat attgatgtcc agtcccgtac
    52981 tctacgcgcc agagggaaat tcacctggcg cgtatttttg ttcgcggctt agctacggct
    53041 gagcacgccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg
    53101 cggttcgtcc aggcttgcca ggacaatctc cagcacacgc tctgcgacat cgcggtgacg
    53161 ttgagccact gccagcaccg gacactgtaa gaagtcgagc agttcgttat cgccaaaggt
    53221 ggcaattgcc aggtcagaag gcagtttgcc gtcgcgacgc agcgtgacat ccatcactcc
    53281 ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg tttccagcca
    53341 tttttcgaat aactgggcag ccgcctcccg ctcatagctg ttggcataca ggaaatgcac
    53401 ttcgcgcgga tcatctttcc aagcagtacg gaaaccttgt tcacgcagga agctgacaga
    53461 aagctccggc agcgcgccaa gataaagcac cgtctcggcg ggaaacttac gtaactcttc
    53521 cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtgaaat gttcacgatc
    53581 gagggcgcgg tccagcgcga caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg
    53641 atgctcaggt ggcaacgacg tcgaaacaat aatggcatca acctgacgct gtaaaaggtg
    53701 ctcaatgcag cgcatttcgt tgtctggctg atcttctgag caggcaatca gcagttgata
    53761 accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc tggtgttctc
    53821 cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc cagctgccac
    53881 ggcgttcggg tggtaattgt gctcacgcac cacagccatg actttttcaa cggttttgtc
    53941 gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg tccgcgacac
    54001 tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa
    54061 ccatagccat tgtactggta tgggttaaat tcattaacat ctaagcgcag aaaattaact
    54121 gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc aacgtgacat
    54181 gttgggctgc aaattgcgca ctgagatcat tctcaacgca ttattttatc gccgcgcgaa
    54241 agtccgacca caccagagcg agcaacttcc acaattttcg ccacatcgcg aatcgatgct
    54301 aaaaatgcat caagcttatc gctggtgcct gctaattgaa cggtataaag cgagggggtg
    54361 acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa
    54421 ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc ctgccccaac
    54481 tcactcacgc gcaagacatc gaccagcttg tgtaattgct tttcgatctg ctcaagtact
    54541 ttttcatcgc ccacggtctg gatggtcata cgcgataatg tctgatcgtc ggttggcgca
    54601 acggtcaggc tttcaatgtt gtagcctcgc tgggaaaaaa ggccaatcac gcgggataac
    54661 gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag gttctctccg
    54721 ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg tagacgtgct
    54781 cactgccatc gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc tgttccagcg
    54841 cctcgctaag tttgctttcc agctcatgcg gatgagagat ctggatcccg acgtgaccat
    54901 aggcttccgc cagacggacg aaatcgggta gtgattgcat ataagattgt gaatgacggc
    54961 cggaatagat catgtcctgc cactgtttca ccatccccag atagcggtta ttgagattca
    55021 ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctggatg ttcatctgaa
    55081 tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt ttgacgccca
    55141 gtgccgcagg taaaccaaaa cccatcgtgc cgagaccgcc ggaattgatc cagcgacgcg
    55201 gtttgtcgaa tggataataa agcgcagcaa acatctggtg ctgcccgaca tcggacgtca
    55261 cgtaagcgtc cccattagtc aaccgccaaa gagtctcgat caccgcctgc ggtttaatct
    55321 tttcactgtg agtgtcatat ttcaggcact gaagagcgcg ccactgttca atctgctgcc
    55381 accagtcgcg gatctcatcc agcggttgat gggcggattc ttgcgacagg agttcaagca
    55441 tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc acggttttag
    55501 aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag tactttgcca
    55561 gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat
    55621 gcatcgtcat attggcttcg taggtaccgt gcattcccag cattcccagt gcctgacgat
    55681 gcgttgccgg aaacgccccc agccccatca atgaggaaac aaagggcaga ttcaacgcct
    55741 ccaccgtttc tttcaactgc tgatggcagc ccgccatgat tgccccaccg cctacgtaga
    55801 caaccggttt ttttgccgcg accagcgttt gcagagcacg cttaatttgc cctttatgtc
    55861 cggtagtagt gggattgtaa gaacgcatac tgaccgactc agggcagaca tagggtaatt
    55921 tgttcgccgg attaagaata tctttcggta aatcaacgac cactggtcct gggcgaccac
    55981 ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt tgcttaacca
    56041 gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat
    56101 cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga atggaatcca
    56161 tataagcggt ggcgatgccg gtaatcgcat tggtcgcccc tggacccgac gttaccagca
    56221 cgacgccgac ttccccggtc gcacgcgcca ggccatcggc catatgcacc gccgcctgct
    56281 catgacgaac taatacatga tcaataccac ccacggtatg caatgcatcg taaatatcaa
    56341 ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata agcgatcgga
    56401 cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga cggaaaaaat
    56461 gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga gacataaacg
    56521 cagggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc ctccattacg
    56581 aaggattaaa ttgataataa atcggctgaa tcccacaact tacagcaatt tctctatttt
    56641 tttaattaat cctaaaatag agagtccaca cattcagaat aaaattctac attcaataaa
    56701 aaatgcagaa taaaccagac attcatgtct ggcctattct gcattcagtt agcgtttgca
    56761 aattgagact aattgttctt ccatccactg atggccttta tcgcgcccgg cagcttcatg
    56821 ccaggagaga taacaggttc tgctgttttg ttttaacggc agcggtaata cctgtaattc
    56881 taaggattca gcgaactctt cagccagcca acgcggcgca atagcgacca aatgcgtttg
    56941 cgacaccacg ctaagtacgc tcatcattgc catgccctga tacgcgatac tggcttgctt
    57001 atctaccgtg tcataccaag gttgactaaa tgacgcgaaa cgatcgagcg aaaccgccgc
    57061 atgttgttcg ttataaacat catgtttcag taacgggcct ttaattgttg gatgattttt
    57121 gctggctacc agcaccattt catctttaaa taatggcacg ctggtaaatt caggacgatg
    57181 gaagtcttca taactaatca caaactccgt ttcctgataa cgcagctgat gttcagtgtt
    57241 ctgatttaat gaagacttga acataacatg tatatttggc gcaatctgct caatgtgatt
    57301 ataaatctgc gaggtcagaa tgctgtctaa cgggctgcaa acacaaagat gaaatacacg
    57361 ttcactgctc gcgggttcaa aacctgaact aggcaattca ttttgtacta gttgcaatgc
    57421 ctggcgaact gaaccaaaaa gttgaaatgc gcgagcagtc ggttgaatac cacggccata
    57481 acgaacaaaa agctcgtcat taaacatcac cttcaggcgt gcaacagcgt tactgaccgc
    57541 aggttgcgac atacccagaa catgagcggc acgggtaatg ttttgctcct gcatcacggc
    57601 atcgaaaacg gttaataagt tgagatcgac catgcgtagc tgtggcttgc ttaactccgc
    57661 cgtctctgaa tgatctgttt gtacctctgg catacttaac tccactgtca cacttaactc
    57721 cctttccctt attggaatgc agagaaataa tcctgaagaa tatgatttat catgcatata
    57781 aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg ttctgataaa
    57841 aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc ataatgtttt
    57901 tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac aaaatggatt
    57961 aaatgtgctt atttaataat taatttatat atttaatgca ttaattctta acattaattg
    58021 atcaataata ttcaccaaat caatatcaaa aaaaatcgca aaacatataa ttcaatacaa
    58081 atcatcagga taggttttgc aacgcgtgca ttttgtcccc tttttcctcg ttgattagat
    58141 gcaaaaattt atgctgaaat atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt
    58201 aatatcgctt actttaacca ccgtagcaca attagctaat tttacggatg cagaactcac
    58261 gctggcggga cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc agtaactcta
    58321 aaagcatatc gcattcatct ggagctgatt taatgactca catcgttcgc tttatcggtc
    58381 tactactact aaacgcatct tctttgcgcg gtagacgagt gagcggcatc cagcattaag
    58441 ccagcacgca gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg
    58501 aggcgctcta aaagagacaa ggacccaaac catgagccag caagtcatta ttttcgatac
    58561 cacattgcgc gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca
    58621 aattgcgctg gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc
    58681 ttcgccgggc gattttgaat cggtgcaaac catcgcccgc caggttaaaa acagccgcgt
    58741 atgtgcgtta gctcgctgcg tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt
    58801 cgccgaagcc ttccgtattc atacctttat tgctacctcc ccaatgcaca tcgccaccaa
    58861 gctgcgcagc accctggacg aggtgatcga acgcgctatc tatatggtga aacgcgcccg
    58921 taattacacc gatgatgttg aattttcttg cgaagatgcc gggcgtacac ccattgccga
    58981 tctggcgcga gtggtcgaag cggcgattaa tgccggtgcc accaccatca acattccgga
    59041 caccgtgggc tacaccatgc cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg
    59101 cgtacctaac atcgacaaag ccattatttc cgtacatacc cacgacgatt taggcctggc
    59161 ggtcggcaac tcactggcgg cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa
    59221 cgggatcggc gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg cgattaaagt
    59281 tcgtaaggat attctcaacg tccacaccgc cattaatcac caggagatat ggcgcaccag
    59341 ccagttagtt agccagattt gtaatatgcc gatcccggca aacaaagcca ttgttggcag
    59401 cggcgcattc gctcactcct ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa
    59461 ctacgaaatc atgacaccag aatctattgg tctgaaccaa atccagctga atctgacctc
    59521 tcgttcgggg cgtgcggcgg tgaaacatcg catggatgag atggggtata aagaaagtga
    59581 atataattta gacaatttgt acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt
    59641 gtttgattac gatctggagg cgctggcctt catcggtaag cagcaagaag agccggagca
    59701 tttccgtctg gattacttca gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt
    59761 caaactggcc tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg gtccggtcga
    59821 tgccgtctat caggcaatta accgcatcac tgactataac gtcgaactgg tgaaatacag
    59881 cctgaccgcc aaaggtcacg gtaaagatgc gctgggtcag gtggatattg tcgccaacta
    59941 caacggtcgc cgcttccacg gcgtcggcct ggccaccgat attgtcgagt cctccgccaa
    60001 agccatggtg cacgtactga acaatatctg gcgtgccgca gaagtcgaaa aagagttgca
    60061 acgcaaagct caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat
    60121 attgccgtat tgccggggga cggtattggt ccggaagtga tgacccaggc gctgaaagtg
    60181 ctgaatgccg tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta cgatgtaggc
    60241 ggcgcagcca ttgataacca cgggcaacca ctgccgcctg cgacggttga aggttgtgag
    60301 caagccgatg ccgtgctgtt tggctcggta ggcggcccga agtgggaaca tttaccacca
    60361 gaccagcaac cagaacgcgg cgcgctgctg ccgctgcgta agcacttcaa attattcagc
    60421 aacctgcgcc cggcaaaact gtatcagggg ctggaagcat tctgtccgct gcgtgcagac
    60481 attgccgcta acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg catctatttc
    60541 ggtcagccaa aaggccgcga aggtagcgga caatatgaaa aagcgtttga taccgaggtg
    60601 tatcaccgtt ttgagatcga gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt
    60661 cgccacaaag tcacctcaat cgacaaagct aacgtgctgc aatcctctat tttatggcgt
    60721 gaaatcgtca acgagatcgc cacggaatac ccggatgtcg aactggcgca tatgtacatc
    60781 gacaacgcca ccatgcagct gattaaagat ccatcacagt ttgacgttct gctgtgctcc
    60841 aacctgtttg gcgacattct gtctgacgag tgcgcaatga tcactggctc gatgggcatg
    60901 ttgccttccg ccagcctgaa cgagcaaggt tttggactgt atgaaccggc gggcggctcg
    60961 gcaccagata tcgcaggcaa aaacatcgcc aacccgattg cacaaatcct gtcgctggca
    61021 ctgctgctgc gttacagcct ggatgccgat gatgcggctt ccgccattga acgcgccatt
    61081 aaccgtgcat tagaagaagg cattcgcacc ggggatttag cccgtggcgc tgccgccgtt
    61141 agtaccgatg aaatgggcga tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg
    61201 ctaaaacgtt atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa
    61261 ccccgctgtt atatatcgac cgccacctag tgcatgaagt gacctcaccg caggcgttcg
    61321 atggtctgcg cgcccacggt cgcccggtac gtcagccggg caaaaccttc gccactatgg
    61381 atcacaacgt ctctacccag accaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc
    61441 agatgcagga gctgattaaa aactgcaaag aatttggcgt cgagctgtat gacctgaatc
    61501 acccgtatca ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga
    61561 tgaccattgt ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc gcactggcct
    61621 ttggtatcgg cacttccgaa gttgaacacg tactggcaac acaaaccctg aaacagggcc
    61681 gtgcgaagat catgaaaatt gaagtccagg gcaaagccgc gccgggcatt accgcaaaag
    61741 atatcgtgct ggcaattatc ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg
    61801 agttttgcgg cgaagcaatt cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata
    61861 tggcgatcga aatgggcgca aaagccggtc tggttgctcc tgacgaaacc acctttaact
    61921 atgtcaaagg ccgtctgcat gcgccgaaag gcaaagattt cgacgacgcc gttgcctact
    61981 ggaaaaccct gcaaaccgac gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag
    62041 aagaaatttc accgcaggtc acctggggca ccaatcccgg ccaggtgatt tccgtgaacg
    62101 acaatattcc cgatccggct tcgtttgccg atccggttga acgtgcgtcg gcagaaaaag
    62161 cgctggccta tatggggctg aaaccgggta ttccgctgac cgaagtggct atcgacaaag
    62221 tgtttatcgg ttcctgtacc aactcgcgca ttgaagattt acgcgcggca gcggagatcg
    62281 ccaaagggcg aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc tctggcccgg
    62341 taaaagccca ggcggaagcg gaaggtctgg ataaaatatt tattgaagcc ggttttgaat
    62401 ggcgcttgcc tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg
    62461 aacgttgtgc atccaccagc aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca
    62521 cgcatctggt cagcccggca atggctgccg ctgctgctgt gaccggacat ttcgccgaca
    62581 ttcgcaacat taaataagga gcacaccatg gcagagaaat ttatcaaaca cacaggcctg
    62641 gtggttccgc tggatgccgc caatgtcgat accgatgcaa tcatcccgaa acagtttttg
    62701 cagaaagtga cccgtacggg ttttggcgcg catctgttta acgactggcg ttttctcgat
    62761 gaaaaaggcc aacagccaaa cccggacttc gtgctgaact tcccgcagta tcagggcgct
    62821 tccattttgc tggcacgaga aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg
    62881 gcattgaccg actacggttt taaagtggtg attgcgccga gttttgctga catcttctac
    62941 ggcaatagct ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa
    63001 ctgtttgcgc tggtgaaagc caatccgggg atccatttcg acgtggatct ggaagcgcaa
    63061 gaggtgaaag cgggagagaa aacctatcgc tttaccatcg atgccttccg ccgccactgc
    63121 atgatgaatg gtctggacag catcggcctg accctgcagc acgacgacgc tattgcgtct
    63181 tatgaagcaa aacaacctgc gtttatgaat tagacccctt gcccggtcaa atgaccgggc
    63241 tttccgctat cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac
    63301 aacagaaata atcgcaatta cccagtagac agcaaagtgc ccccaacttt gtgcaattgc
    63361 tccctgaata acaccagcca gaattacccc ggtagagata gtgttggtaa ataaggtggt
    63421 agctgccccc gctcttccag gcattaaatc ctgaaaccat agcatcccaa tacccgcaac
    63481 aatgccgata aatacagcgt taaaaagttg cagcgtcatc aacgccatac ggctatgaaa
    63541 gaaaatcaat ccagtgtaaa acagcactcc tgccaccact gcaatgacca tcattcgccg
    63601 cttaccataa cgtttgacat agtagccagc cagaatcatt gctggtattt ccagtccagc
    63661 tgccgtcccc atcaggaaac ccgccagttt gtctggcaat cctaaatcgc tactgatcca
    63721 caacggcata tcaataatgt acatagtgtt gcaggtccac attaacgtcg aggcgacaaa
    63781 taacatccgt acgttactat cctgccagtc accttgcatt gataaagcat tttccgacgg
    63841 cagttctacc cgcgccacag acggaagcat aaatgcaatc aataccagac tgagtgtgaa
    63901 tatcccggcg gcaatcgaaa acatcactgt aaagccgtaa ttcaacgcca gcataaaggc
    63961 cagcggtgga ccgattaccc atgccagaga aagctgcgca cgcatcaccg agctaaacat
    64021 caccacttct cgcgccgagt tatccgcgta ttcccgcgcc agagcaaata actgtggcat
    64081 tgccgtattg gccagagatg ccagaagcac accacaggtg ataagcgtca gataatgacg
    64141 attaaatgca aacagtagcg cattgccaat agccatcaaa cagcaaaata taatcagttt
    64201 tcgccgatcg ccctgactgt cagaacgttt tgccaaccag aggcttacgc cgatcccagc
    64261 aatagcattc accgtataaa agagtccgat ccagaaaggt tgcgcgccaa cctcacgact
    64321 cagaaataag ctcaatgtag gagcctgtag cgccccggcc acccccatca taaaagcgac
    64381 cagcataaat gccgcgtaaa caccgttcat acggcgagcc atcgtcatta tccagatcat
    64441 acgttccctt tttagcgcgg cgagaataaa aaaaaccagc aggtataatc tgctggcggg
    64501 tgattttaca ccaatactca gtcacacatg atgcaggcaa gtcaactttc agaattgcgg
    64561 tcatcccact gcatcagtcc ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc
    64621 aaccagcaca acttcgctgt cgcggtaaaa tagtgctgat aaaactgacg catggggcac
    64681 ctccttgctt catcgttagg aattattgac ttaatatagg gaaaataaaa ttgctgtctt
    64741 ttgcgcagga gttccccttt tatgccatct gctcgtctgc aacaacagtt catccgcctg
    64801 tggcaatgct gcgagggtaa atcgcaggac acaacgctca acgaactggc agcgttattg
    64861 agctgctcgc gtcgtcatat gcgtaccctg ctcaacacca tgcaggatcg cggctggctg
    64921 acgtgggaag cggaagtcgg gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg
    64981 ctggcgcttc agcaacagcg ggcggaagac ctgctggagc aggatcgtat cgaccaacta
    65041 gtgcagttgg ttggcgacaa agcgactgtg cggcaaatgc tggtttctca tctgggccgc
    65101 agcttccgcc aggggcggca catcctgcgc gtgctctact atcgtccgtt gcgtaatctg
    65161 ctacctggca gcgcattgcg ccgttccgaa acccatattg cccggcaaat cttcagttcg
    65221 ctaacgcgca taaatgagga aaatggggaa ctggaagcag acatcgccca ccactggcag
    65281 caaatatcac cgcttcactg gcgtttcttt ttgcgtccag gagtccattt tcatcatggt
    65341 cgtgaactgg aaatggacga cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc
    65401 tattcgcata ttacggacat tgtgtcgccg acgccctgga cgctggatat ccatctcacg
    65461 cagccggacc gctggttgcc gttactgctg gggcaagttc cggcgatgat cctgccgtgc
    65521 gaatgggaaa ccctcagtaa ctttgccagc catcctattg gcaccggtcc gtatgcggtg
    65581 attcgcaaca gcaccaatca actgaaaatt caggcattcg atgacttctt cggttaccgg
    65641 gcattaatcg acgaagttaa cgtctgggtt ctgccggaaa ttgccgacga gccagccggt
    65701 ggtctgatgc taaaaggtcc acagggcgag gaaaaagaga ttgaaagccg cctggaggaa
    65761 ggttgctact atttactgtt cgacagccgc acccatcgcg gggcgaatca gcaagtcagg
    65821 gactgggtaa gctatgtgct ttctccaact aatctggtct atttcgctga ggaacagtac
    65881 cagcaactgt ggttcccggc ttatggactg ctcccccgtt ggcaccatgc ccgcaccata
    65941 aagagcgaaa aaccggctgg cctggaaagc ctcaccctga ccttttatca ggatcacagt
    66001 gagcatcggg tgattgccgg gatcatgcag cagattctgg caagtcacca ggtcacgctg
    66061 gaaatcaaag agatcagcta cgatcagtgg catgaaggag agatcgagag cgatatctgg
    66121 cttaatagcg ccaactttac gctgccgctg gatttttcgc tgttcgcgca cttgtgcgag
    66181 atgccgctgc tccaacactg tattccgatc gactggcaag ccgacgccgc ccgctggcgc
    66241 aatggcgaaa tgaacctggc gaactggtgc cagcaactgg tcgccagtaa agcaatggtg
    66301 ccgcttatcc accactggct gatcattcag ggacaacgca gtatgcgcgg cctgcgtatg
    66361 aataccctcg gctggttcga ttttaaatca gcgtggtttg cgccaccgga tccatgattg
    66421 ctggtagata acaaaatcac tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg
    66481 tgcgctgaga aaatacccgt cgaacctgat ccggataacg ccggcgaagg gatttgaggc
    66541 tccttctcaa gtcctttgcc actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc
    66601 cctgctgttg ctgtgcacag cgcccgtttt cgctaaaccc gttctgactg tttataccta
    66661 cgattccttc gccgccgact gggggccagg tccaaagatc aaaaaagctt ttgaagccga
    66721 ctgtaactgc gaactgaaac tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct
    66781 acggatggaa ggcaaaaata gtaaagccga tgtggtgctg gggctggata acaatctgtt
    66841 agacgccgcc agtaaaaccg gactatttgc caaaagcggt gtggcagcgg atgccgttaa
    66901 cgttcccggc ggctggaata atgacacttt cgtcccgttt gattacggct atttcgcctt
    66961 cgtctatgac aagaacaaac tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag
    67021 cgatcagaac tggctggtga tttatcagga tccgcgtacc agcacgccgg ggctgggtct
    67081 gctgttgtgg atgcaaaaag tctatggcga tgacgcccca caagcctggc aaaaactggc
    67141 gaagaaaacc gtcacggtca ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg
    67201 tgaaagcaat ctggtactga gttacaccac ctctccggct tatcacattc tcgaagagaa
    67261 aaaagataac tacgccgccg cgaactttag cgaaggtcac tatctgcagg tggaagtcgc
    67321 cgcccgcacc gctgccagca agcagccgga gctggcgcaa aaattcctcc agtttatggt
    67381 ttctccggct tttcagaatg cgatcccaac cggtaactgg atgtatccgg tggcaaatgt
    67441 cacgctgcct gccgggtttg aacaattgac caaaccggcc accacgctgg agttcacacc
    67501 agctgaagtg gcggcacaac gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg
    67561 ttaattcccg gctggttaat tccaagtgta agcgccgcca cgctggtggt agcggttgcg
    67621 atcgcggcgt ttctcgccct gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg
    67681 caggacagct acctgtggca tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg
    67741 ctactctctg tcgtacccgc gatattcctc gcccgcgccc tctatcgcag gcgctttccg
    67801 ggtcggctgg cgctgttgcg tctgtgcgca atgaccttga tcctcccggt gctagttgct
    67861 gttttcggca ttcttagcgt ctatggtcgt cagggctggc tagcatcgct ctgccaatcg
    67921 ctcggtctgg agtggacctt ttcgccctac ggcctgcaag gcattttgct ggcgcacgta
    67981 ttttttaatc tgccgatggc gagccgctta ttactccagg cactggaaaa cattcccggc
    68041 gaacaacgtc agcttgccgc ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc
    68101 gaatggccgt ggctacggcg acaaatcccg ccggttgccg cgcttatctt tatgctctgt
    68161 ttcgccagct tcgccaccgt gctatcgctg ggcggcggtc cgcaggcaac cactatcgag
    68221 ctggctattt atcaggcgct gagttacgac tacgatcctg cccgcgcggc gatgctggcg
    68281 ctgctccaga tggtgtgttg ccttgggctg gtgctgttga gtcagcgact aagtaaggcc
    68341 attgcgcccg gcaccacgct gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc
    68401 cgcatttgcg acacggtgtt aatagtgctg gcgctgctgc tgttgctgcc accgttgctg
    68461 gcggtgatcg tcgatggggt aaatcgccag ttgccggaag tgctggcaca accggtgctg
    68521 tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg
    68581 ctgaccatga tgctgctatg gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg
    68641 ggtcaggcgc tggagatgag cggcatgttg atcctcgcca tgccggggat tgtgctggct
    68701 accggcttct ttttactgct caacaacact atcggcctgc cacaatctgc tgacggcatt
    68761 gtgattttca ccaatgcgtt aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg
    68821 atgcgcgata tcaccgcccg ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg
    68881 tcgcgcttaa aagtggtcga gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct
    68941 tttgcctgcg tgctgtcgat tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat
    69001 ttccgtaccc tgccgtttta tctctaccag caaattggct cctatcgcag ccaggacggc
    69061 gcggtcaccg cgttaattct gctactactc tgttttctgc tgtttaccgt gattgaaaaa
    69121 ctaccggggc gaaatgttaa aactgactga tatcacctgg ctttatcacc atttgccgat
    69181 gcgttttagc ttaacggtag aacgcggcga gcaggtggca atcctcgggc caagcggcgc
    69241 gggtaaaagt accctgctga atttgattgc cggttttctg acgccagcca gcggttcgct
    69301 gactatcgat ggcgttgatc acaccacgac gccgccgtca cgccgtccgg tgtcgatgct
    69361 gtttcaggag aacaacctgt tcagccacct gacggtcgcg cagaacatcg ggctggggct
    69421 aaatccgggg ttgaaactga acgcggcaca gcaggagaaa atgcacgcga ttgcccgtca
    69481 gatggggatt gataatttaa tggcgcggtt accgggcgag ctttccggcg gtcagcgaca
    69541 gcgcgtggcg ttagcgcgtt gtctggtgcg cgaacagccg attttattgc tcgatgaacc
    69601 gttctctgcg ctcgatccgg cgttacgtca ggagatgctg acgctggtga gcacgagctg
    69661 ccagcagcaa aaaatgacgc tattgatggt gtcgcacagt gtggaagatg cggcgcggat
    69721 cgccacacgc tcggtggtgg tcgccgacgg gcgcatcgcc tggcagggta agactgaaga
    69781 attgttgagc ggtaaggcga gtgcttcggc aatattgggg attacgggtt agtatgcact
    69841 gtcagatgcg gcgtgaacac cttatccgac ctacggacag tgcgttttgt aggcctgata
    69901 agacgcggtc agcgtcgcat gaggcaaccc gcacaagaca atcccctaaa ccccaaccac
    69961 tttacgcaaa atatcgatat acaccggcat caacgggtgg cgaattaaca ccaccagcgc
    70021 caccacgccg atggcagaaa tcaacggcgt cagccacaac aaacggccgc gcgacaaata
    70081 atgacttaaa cgatcggttg ctttaccgct gcgccataac cgccagcaca gccagccacc
    70141 aacccacaaa aataccgccg tcgccagcag caaccattta aactcgccgc tctgcattcc
    70201 ggctggaata tcgatcgccg cgcccgccag aatcccaggc aggaagtaaa acggtggcca
    70261 cagcaggcag ccgataatat tcggcgtaat gaattttgcc accggcagat ccagcattcc
    70321 cgccaccatt ggcaccagcg gacgcgtcgg gccaacaaag cgaccgacca gaatggtgaa
    70381 catgctgtgt tgatgcaacg catgttcagt tttatcgagt aatgctttgt ttttcttcag
    70441 aaatgaccag cgatgcaacg gctttttaaa acgccagccc agccagaagg agatccagtc
    70501 gcccagcaga caaccgacaa tccctgccag ccaggcgtgc cagaaactta actcgccgct
    70561 gccaatcagc gctcccagcc ccgccatcag caccgtaccg ggtagaatca aaccgaccag
    70621 cgccagcgac tccagaaagg ccaccaacac caccgccatc aatgaataca cggtagattg
    70681 ggtaataaag tgttccagca atgcttgcat aatgtgcctg tcaaatggac gaagcaggga
    70741 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt actaattatg
    70801 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
    70861 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
    70921 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
    70981 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
    71041 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
    71101 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac acgattatcc atcggtggat
    71161 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
    71221 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
    71281 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa acccgtattg gcaaatattg
    71341 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
    71401 accattcgcg agcctccgga tgacaaccgt agtgatgaat ctctcctggc gggaacagca
    71461 aaatatcacc cggtcggcag acaaattctc gtccctgatt tttcaccacc ccctgaccgc
    71521 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
    71581 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
    71641 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
    71701 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
    71761 tctcgctaac ccaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
    71821 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
    71881 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
    71941 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttgga
    72001 tggagtgaaa cgatggcgat tgcaattggc ctcgattttg gcagtgattc tgtgcgagct
    72061 ttggcggtgg actgcgccag cggtgaagag atcgccacca gcgtagagtg gtatccccgt
    72121 tggcaaaaag ggcaattttg tgatgccccg aataaccagt tccgtcatca tccgcgtgac
    72181 tacattgagt caatggaagc ggcactgaaa accgtgcttg cagagcttag cgtcgaacag
    72241 cgcgcagctg tggtcgggat tggcgttgac agtaccggct cgacgcccgc accgattgat
    72301 gccgacggta acgtgctggc gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc
    72361 gtattgtgga aagaccacac tgcggttgaa gaagcggaag agattacccg tttgtgccac
    72421 gcgccgggca acgttgacta ctcccgctat attggcggta tttattccag cgaatggttc
    72481 tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg tggcgcaatc tgccgcatcg
    72541 tggattgagc tgtgcgactg ggtgccagct ctgctttccg gtaccacccg cccgcaggat
    72601 attcgtcgcg gacgttgcag cgccgggcat aaatctctgt ggcacgaaag ctggggcggc
    72661 ttgccgccag ccagtttctt tgatgagctg gacccgatcc tcaatcgcca tttgccttcc
    72721 ccgctgttca ctgacacctg gactgccgat attccggtgg gcaccttatg cccggaatgg
    72781 gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat
    72841 atgggcgcag ttggcgcagg cgcacagcct aacgcactgg taaaagttat cggtacttcc
    72901 acctgcgaca ttctgattgc cgacaaacag agcgttggcg agcgggcagt taaaggtatt
    72961 tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg gtctggaagc aggccaatcg
    73021 gcgtttggtg atatctacgc ctggtttggt cgcgtactcg gctggccgct ggaacagctt
    73081 gccgcccagc atccggaact gaaagcgcaa atcaacgcca gccagaaaca actgcttccg
    73141 gcgctgaccg aagcatgggc caaaaatccg tctctggatc acctgccggt ggtgctcgac
    73201 tggtttaacg gtcgtcgcac gccaaacgct aaccaacgcc tgaaaggggt gattaccgat
    73261 cttaacctcg ctaccgacgc tccgctgctg ttcggcggtt tgattgctgc caccgccttt
    73321 ggcgcacgcg caatcatgga gtgctttacc gatcagggga tcgccgtcaa taacgtgatg
    73381 gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg
    73441 aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt
    73501 gctgccgtcg ccgcgaaagt gcacgcagac atcccatcag cccagcaaaa aatggccagt
    73561 gcggtagaga aaaccctgca accgcgcagc gaacaggcac aacgctttga acagctttat
    73621 cgccgctatc agcaatgggc gatgagcgcc gaacaacact atcttccaac ttccgccccg
    73681 gcacaggctg cccaggccgt tgcgactcta taaggacacg ataatgacga tttttgataa
    73741 ttatgaagtg tggtttgtca ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca
    73801 ggtcacccaa catgccgagc acgttgttaa tgcgctgaat acggaagcga aactgccctg
    73861 caaactggtg ttgaaaccgc tgggcaccac gccggatgaa atcaccgcta tttgccgcga
    73921 cgcgaattac aacgatcgtt gcgctggtct ggtggtgtgg ctgcacacct tctccccggc
    73981 caaaatgtgg atcaacggcc tgaccatgct caacaaaccg ttgctgcaat tccacaccca
    74041 gttcaacgcg gcgctgccgt gggacagtat cgatatggac tttatgaacc tgaaccagac
    74101 tgcacatggc ggtcgcgagt tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt
    74161 cgttaccggt cactggcagg ataaacaagc ccatgagcgt atcggctcct ggatgcgtca
    74221 ggcggtctct aaacaggata cccgtcatct gaaagtctgc cgatttggcg ataacatgcg
    74281 tgaagtggcg gtcaccgatg gcgataaagt tgccgcacag atcaagttcg gtttctccgt
    74341 caatacctgg gcggttggcg atctggtgca ggtggtgaac tccatcagcg acggcgatgt
    74401 taacgcgctg gtcgatgagt acgaaagctg ctacaccatg acgcctgcca cacaaatcca
    74461 cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt
    74521 cctggaacaa ggtggcttcc acgcgttcac caccaccttt gaagatttgc acggtctgaa
    74581 acagcttcca ggtctgcccg tacagcgtct gatgcagcag ggttacggct ttgcgggcga
    74641 aggcgactgg aaaaccgccg ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca
    74701 gggcggcacc tcctttatgg aggactacac ctatcacttc gagaaaggta atgacctggt
    74761 gctcggctcc catatgctgg aagtctgccc gtcgattgcc gtagaagaga aaccgatcct
    74821 cgacgttcag catctcggta ttggtggtaa ggacgatcct gcccgactga tcttcaatac
    74881 ccaaaccggt ccagcgattg tcgccagcct gattgatctc ggcgatcgtt accgtctgct
    74941 ggttaactgt atcgacacgg tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa
    75001 tgcactgtgg aaagcgcaac cggatctgcc aactgcttcc gaagcgtgga tcctcgctgg
    75061 tggcgcgcac cataccgtct tcagccatgc gctgaacctc aacgatatgc gccagttcgc
    75121 cgagatgcac gacattgaaa tcacggtgat tgataacgac acccgcctgc cagcgtttaa
    75181 agacgcactg cgctggaacg aagtgtatta cggatttcgt cgctaagtag ccacatcagg
    75241 tatgtaacgc ctgatgcgac gctgacgcgt cttatctggc ctacacgctg cgattttgta
    75301 ggccggataa gcaaagcgca tccggcattc aacgcctgat gcgacgctgg cgcgtcttat
    75361 caggcctacg cgctgcgatt ttgtagaccg gataagcgaa gcgcatccgg cacgaaggag
    75421 tcaacatgtt agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac
    75481 acaacctggt cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct
    75541 ttgtgatcaa accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg
    75601 ttagcatcga aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc gacacgccaa
    75661 ctcaccggct gctctatcag gcattcccct ccattggcgg cattgtgcat acgcactcgc
    75721 gccacgccac catctgggcg caggcgggtc agtcgattcc agcaaccggc accacccacg
    75781 ccgactattt ctacggcacc attccctgca cccgcaaaat gaccgacgca gaaatcaacg
    75841 gcgaatatga gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa cagggtatcg
    75901 atgcagcgca aatgcccggc gttctggtcc attcccacgg cccgtttgca tggggcaaaa
    75961 atgccgaaga tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggggatat
    76021 tctgccgtca gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact
    76081 atctgcgtaa gcatggcgcg aaggcatatt atgggcagta atgactgtat aaaaccacag
    76141 ccaatcaaac gaaactaggc tatactcaag cctggttttt tgatggattt tcagcgtggc
    76201 gcaggcaggt tttatcttaa cccgacactg gcgggacacc ccgcaaggga cagaagtctc
    76261 cttctggctg gcgacggaca acgggccgtt gcaggttacg cttgcaccgc aagagtccgt
    76321 ggcgtttatt cccgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg
    76381 ctttcgtctg acaccgctgg cgttaaagga ttttcaccgc cagccggtgt atggccttta
    76441 ctgtcgtgcc catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac
    76501 cgtctacgag gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc
    76561 accggtgtgg gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc
    76621 gcatcccgac tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca
    76681 cggtgagctg tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg
    76741 gccggagaat ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc
    76801 gctgctgctg gaaaaactca acgcctggtt tgcaaaccac gatcccgatg tgatcatcgg
    76861 ctggaacgtg gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct
    76921 tccgctgcgt cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa
    76981 cggcgtcttt tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa
    77041 atccgcgttc tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg
    77101 cgaaggaaaa tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc
    77161 cgaagataaa cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat
    77221 cttccacaaa actgaaatca tgccattttt actcgaacga gcaacggtga acggcctgcc
    77281 ggtggatcgg cacggcggtt cggtggcagc gtttggtcat ctctattttc cgcgaatgca
    77341 tcgcgctggt tatgtcgcgc ctaatctcgg cgaagtgccg ccgcacgcca gccctggcgg
    77401 ctacgtgatg gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag
    77461 cctgtacccg tcgatcatcc gcacctttct gattgatccc gtcgggctgg ttgaaggcat
    77521 ggcgcaacct gatccagagc acagtaccga aggttttctc gatgcctggt tctcacggga
    77581 gaaacattgc ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg
    77641 tcagggtaat aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt
    77701 gctcggcacc accgcctgcc gcttcttcga tccgcgcctg gcatcgtcga tcaccatgcg
    77761 tggtcatcag atcatgcggc agaccaaagc attgattgaa gcgcagggct acgacgttat
    77821 ctacggagat accgactcga cttttgtctg gctaaaaggt gcacattcgg aagaagaggc
    77881 ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca
    77941 aaaacaacgg ctgaccagcg cattagaact ggagtatgaa actcatttct gccgttttct
    78001 tatgccaacc attcgcggag ctgataccgg cagtaaaaag cgctacgccg ggctgattca
    78061 ggagggcgat aagcagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac
    78121 gccgctggcc cagcagtttc agcaggagct gtacctgcgt atcttccgca acgagccata
    78181 tcaggagtac gtccgccaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcggct
    78241 ggtttaccgt aaacgccttc gccgtccgct gagcgaatat cagcgtaatg ttccgcctca
    78301 tgtacgcgcc gctcgccttg ccgatgaaga aaatcaaaag cgcggtcgcc ccttgcaata
    78361 tcagaatcgc ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta
    78421 ccaacgttca ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga
    78481 gggaatactc ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct
    78541 attttgagca aaaaaaagag ttcgccagat accattttga tgcgtgacga atgctttgcc
    78601 atccagtacc atagcgccct ttccattcct ggacctgaat aacaccacta cctcataagc
    78661 acggtagcgg gtggttattg cctgcaatta aagatataga gccgaacaca tatgcctttt
    78721 acacttggtc aacgctggat cagcgataca gaaagcgaat tgggacttgg aaccgttgtc
    78781 gcggtggatg cgcgaactgt cactttactt ttcccatcta ctggtgaaaa ccgtctgtac
    78841 gcacgcagtg attcccccgt gacccgcgtg atgttcaacc ctggtgatac cattaccagc
    78901 catgacggct ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt gctgacctat
    78961 atcggtactc gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat
    79021 agcaaactgg tgttcagcaa accgcaggac cgtctgtttg ccgggcagat tgaccgtatg
    79081 gaccgctttg cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg
    79141 tacagcggcc tgcgcggtca gcgtaccagc ctgatcccgc atcagctcaa catcgctcat
    79201 gatgttggtc gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa
    79261 accattgaag ccgggatgat cctgcatcag caactgctct ctggcgctgc tgaacgtgtg
    79321 ctgattatcg tcccggaaac cttacagcat cagtggctgg tagaaatgct gcgccgtttc
    79381 aacctgcgct ttgctctgtt tgatgatgag cgttatgccg aagctcagca cgatgcttac
    79441 aacccgtttg acaccgaaca gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa
    79501 cagcgcctgg aacatctctg tgaagccgaa tgggacctgc tggtggtcga tgaagcgcat
    79561 cacctggtgt ggagcgaaga tgcgccaagc cgtgaatatc aggccattga acaactggca
    79621 gagcacgtgc cgggcgttct gctgctgacc gcgaccccgg aacagctggg gatggaaagc
    79681 cacttcgccc gtctgcgtct gctggacccg aaccgtttcc acgattttgc gcagttcgtt
    79741 gaagagcaga aaaattatcg tccggttgcg gacgccgttg ccatgctgct ggcaggtaac
    79801 aaactgagca atgacgaact gaacatgctc ggcgagatga tcggcgagca ggatatcgag
    79861 ccgctgttgc aggcagcaaa cagcgacagc gaagatgccc agagcgcccg tcaggagctg
    79921 gtttcgatgc tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa cacgcgtaac
    79981 ggtgtgaaag gattcccgaa acgcgagctg cacaccatta agctgccgct accgacgcag
    80041 tatcagacgg ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgt
    80101 gctcgcgata tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga taacgccacc
    80161 tggtggaact tcgatccgcg cgttgagtgg ctgatgggct acctgaccag ccatcgctct
    80221 cagaaagtgc tggtgatctg cgccaaagct gccactgcgc tgcaactgga gcaggtactg
    80281 cgcgaacgtg aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt
    80341 gaccgcgctg ccgcctggtt tgccgaagaa gacaccggcg cacaggtact gctgtgctca
    80401 gaaatcggtt ctgaaggacg taactttcag ttcgccagcc acatggtgat gtttgacctg
    80461 ccattcaacc cggatctact ggagcagcgt attggtcgtc tggatcgtat cggccaggcg
    80521 cacgatattc agatccatgt gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc
    80581 tggtatcacg aaggtctgga tgcatttgag cacacctgcc cgaccggacg cactatttac
    80641 gatagcgtat acaacgatct gattaactat ctggcttcac cggatcaaac cgaaggcttt
    80701 gacgatctga tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag
    80761 ggtcgtgacc gcctgctgga aatccactcc aacggtggcg aaaaagccca ggcactggca
    80821 gaaagcattg aagagcagga tgacgatacc aacctgatcg ccttcgccat gaacctgttc
    80881 gatattatcg gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc
    80941 gatcatatgc tggtgccgga cttccctggc ctgtcggaag atggcatcac catcaccttt
    81001 gatcgtgaag tggcgctggc gcgtgaagat gcacagttta ttacctggga gcatccgctg
    81061 atccgcaacg gtctggatct gatcctttct ggcgataccg gtagcagcac gatttcactg
    81121 ttaaaaaaca aagcgttgcc agtaggtacg ctgttggtgg aactgattta tgtggttgaa
    81181 gcccaggctc cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg
    81241 ctgctggata aaaacggcaa caacctggcg gcgcaggtag agtttgaaac ctttaaccgc
    81301 cagcttaacg cggttaaccg tcacaccggc agcaaactgg ttaacgccgt gcagcaggat
    81361 gttcacgcta tccttcaact gggtgaagcg cagatcgaga aatctgcccg tgcattgatt
    81421 gatgcagcgc gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct
    81481 ctgcgtgcag tgaacccgaa cattcgtgac gacgaactga ccgccattga gagcaaccgt
    81541 cagcaggtaa tggaaagcct ggatcaggca ggttggcgtc tggatgccct gcgtttgatc
    81601 gttgtaacgc atcagtaacg gagtcgaaaa tggggatgga aaactacaat ccaccgcagg
    81661 aaccctggtt ggttatcctg tatcaggatg accatattat ggtggtcaac aagccgagcg
    81721 gtttgttgtc agtgccgggt cgtctggaag agcacaaaga cagcgtgatg acgcgcattc
    81781 agcgtgatta tccgcaggca gaatcggtgc atcgtctgga tatggctacc agcggcgtga
    81841 ttgtagtggc gctgaccaaa gccgcggagc gggagttaaa acgccagttc cgcgagcgcg
    81901 agccgaaaaa gcagtatgtg gcccgcgtct gggggcatcc atcccccgca gaaggtctgg
    81961 tggatctgcc gctgatttgc gactggccaa accgcccgaa acagaaagtc tgttacgaaa
    82021 cgggtaaacc tgcgcagacg gaatatgaag tggtggagta tgcggcggat aacacggcaa
    82081 gagtggtgtt aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat atgctggcgc
    82141 tggggcatcc gattctcggc gatcgttttt atgcatcacc agaagcgaga gcgatggcac
    82201 cgcgtttgtt gctacatgca gagatgttga cgattaccca tccggcgtat ggcaatagta
    82261 tgacgtttaa agcgccagcg gatttttaat cgtcacgctg gcgcagttcg gtaggcggat
    82321 aaggcgttca cgccgcatcc ggcaattgtg cgccgattgc ctgatgcgac gcgggccgcg
    82381 tcttatcagg cctacagttt aaaacttatt taaacccttt ctgctgcttt atcagctcat
    82441 atgcctgctg aatttcctgc gctttctgct tcgccatctc catcatctca ggcggcaaac
    82501 ctttcgccac cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga
    82561 tggtggtcgc atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgtcg
    82621 ggccacgctg cgcttgctgc cagttaccac cgccagattg ctgctgataa ccgccgccaa
    82681 actgtgcacc gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta
    82741 attcttcagc aatgacatac agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa
    82801 acgccgcctg aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga
    82861 cactgcgaaa ctggcgcatc ttttcgcgca gcgggtaatt atctgatttt cccacccgga
    82921 acgcgttttg cgccgcagta cgggaagcgc catgaaggtt cattcggtcc atcaactggc
    82981 tggcgatatg aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca
    83041 cttcaaaagt ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca
    83101 ttttacggct acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc
    83161 aaaagccgcc gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca
    83221 tatattcccc aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt
    83281 taccgttttc cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct
    83341 gtcattcaat gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta
    83401 gagatgacga gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa
    83461 caataaaatc aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc
    83521 gccctttata gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg
    83581 ccaagctatg accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat
    83641 gctgaccacg cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc
    83701 atgcagggta acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcacta
    83761 ggacaaccgg agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac
    83821 caggtgatcc tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc
    83881 tgggaaggtg attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa
    83941 caacgtggcg aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg
    84001 ggttctgaca cctggagcgt ggtaggtagc gaaattattc atgaccgcga agaacaagtt
    84061 gcggagatct ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat
    84121 ttgcagttgc cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac
    84181 accaccacca actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg
    84241 gatgccacca tcacgccgca ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa
    84301 ttccgctacc tctcccaggc tggcgctggc ttgatggaac tggactatct gccttcagat
    84361 aaagtctatg aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg
    84421 aaccactccg gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc
    84481 gatcctagct acttcaatga tttcgataac aagtacggtt ccagtactga cggctacgca
    84541 acgcaaaaat tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag
    84601 cagttccagg ttttcagcga acagaacacc agtagctact cggcagagcc gcagttagac
    84661 gttaattact accagaatga tgttggtccg tttgatacgc gtatttacgg ccaggcagtg
    84721 cactttgtta acaccagaga cgacatgcct gaagcaaccc gtgttcacct agaaccgacc
    84781 atcaatttgc ctctctctaa taactggggc agcatcaata ccgaagcgaa gtttctggca
    84841 acccattatc agcaaaccaa tcttgactgg tataactcca gaaacacgac caagctggac
    84901 gaatccgtta accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc
    84961 gatatggaaa tgctggctcc gggttatacc caaacgctgg aaccgcgcgc gcagtatttg
    85021 tacgtgccgt atcgcgatca gagcgacatc tataactacg actcgtctct gctgcaatct
    85081 gactactctg gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct
    85141 aaccaggtga cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt
    85201 aatatttccg ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata
    85261 acatgggaga atgacgacaa aacgggctca ctggtgtggg caggcgacac ctactggcgt
    85321 atctccgagc gttggggatt gcgtggcggg attcagtacg atacacgtct ggataacgta
    85381 gcgaccagta actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat
    85441 taccattacg ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct
    85501 gagcaatata agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc aattgccgat
    85561 cgttggtcca ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct
    85621 atgttaggtg tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag
    85681 ctgaacggtt gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac
    85741 atcgaacttc gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg
    85801 aacattctgc cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc
    85861 ggttaattga aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat
    85921 gatcgcgaat accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa
    85981 taacggcgtc gtgctggaaa gcgacgttga tggattaatg cagtcggtaa aactgaacgc
    86041 tgcgcaggca aggcagcagc ttcctgatga cacgacgctg cgccaccaaa tcatggaacg
    86101 tttgatcatg gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga
    86161 tgagcagctg gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca
    86221 gatgcgcggc cgtctggctt acgatggtct gaactacaac acttatcgta accagatccg
    86281 caaagagatg attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct
    86341 gccgcaggaa gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga
    86401 gctgaacctg agccacatcc tgatcccgct gccggaaaac ccgacctctg atcagttgaa
    86461 cgaagcggaa agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg ctgatttcgg
    86521 taagctggcg attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatggggtg
    86581 gggccgtatt caggagttgc cggggatctt cgcccaggca ttaagcaccg cgaagaaagg
    86641 cgacattgtt ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct
    86701 gcgcggcgaa agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa
    86761 accgtcgccg atcatgactg acgaacaggc ccgcgtgaaa ctggagcaaa ttgctgctga
    86821 tatcaagagt ggtaaaacga cttttgctgc cgcagcgaaa gagttctcgc aggatccagg
    86881 ttctgctaac cagggcggtg atctcggctg ggctacacca gatattttcg atccagcctt
    86941 ccgtgacgcc ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt
    87001 cggctggcat ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca
    87061 gaaagatcgt gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg
    87121 gatgcaggaa caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc
    87181 caacgtgttg tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag
    87241 cttgcacagc gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc
    87301 gaccgggcag cgatgctcgg tttgccgctc accctccgcc cttattcccc caactcccct
    87361 gcacaaccgc aaactacggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc
    87421 actgcggggc agttagcgat tgaaaatggg cattacgtgg tggagacgct ggcgcgagcg
    87481 tgtgatggct gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc
    87541 gtcattaacg acgcaagcat tccgtttacc ggtcataccg agtttttcga agagcgttcg
    87601 caggcgaaaa aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg
    87661 acgcatttac cgctgcgcga tatcgcagat gctatcaccc ctgcactttt gcacgaagtg
    87721 attgctattt tgcatcacga tttgcgtaca aaatttggta ttgccgaacc gcgcattctg
    87781 gtctgcgggc tgaatccgca cgcgggcgaa ggcggtcata tgggtacgga agagatagac
    87841 accattattc cggtgctcga cgagctgcgg gcgcagggga tgaaactcaa cgggccgctg
    87901 cctgccgata ccctgtttca gccgaaatat ctcgataacg ccgacgccgt gctggcgatg
    87961 taccacgatc agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt
    88021 acgctgggcc tgccctttat tcgcacatca gtggaccacg gcaccgcgct tgaactggcg
    88081 ggacgtggcg aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg
    88141 attgttaaca cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg
    88201 ggcaaaactt tctcaacgat cacttcgtga tcgacagcat tgtttctgcc attaacccgc
    88261 aaaagggcca ggcaatggtc gaaatcggcc ccggtctggc ggcattgacc gagccggtcg
    88321 gcgaacgtct ggaccagctg acggtcatcg aacttgaccg cgatctggcg gcacgtctgc
    88381 aaacgcatcc attcttaggc ccgaaactga cgatttatca gcaggatgcg atgaccttta
    88441 actttggcga actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt
    88501 ataacatctc cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca
    88561 tgcactttat gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaaag
    88621 cgtatggtcg attaagcgtc atggcgcaat actattgcaa cgtgatcccg gtactggaag
    88681 ttccgccgtc agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctagtcc
    88741 ctcatgcaac gatgcctcac ccggttaaag atgttcgcgt gttgagccgc atcaccaccg
    88801 aagcctttaa ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg
    88861 aggtgttaac cggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc
    88921 aatattgcca gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat
    88981 gatcaattcg ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc
    89041 acctgataat gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc
    89101 gccagtgcag ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga
    89161 agtccatggc gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg aagagtacca
    89221 gtacaccagc ggtgcaatca ttgaaacccc gctgggcaca atgcagggtc actacgaaat
    89281 gatcgatgaa aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc
    89341 cacactcatt cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta
    89401 cgatgaactg atcgcattgc tgcataaagt agaatttacc cccgggaaag ataccctctg
    89461 gctgacgggc gatctggtcg cgcgcggccc gggttcgctg gatgttctgc gctatgtgaa
    89521 atccttaggc gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt
    89581 atttgccggg atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc
    89641 ggatgccgac gagctgctta actggctgcg tcgccagcct ctgctgcaaa tcgacgaaga
    89701 gaaaaagctg gtgatggccc acgcagggat cacgccgcag tgggatctgc agaccgccaa
    89761 agagtgcgcg cgcgatgtag aagcggtgct gtcgagtgac tcctatccct tctttcttga
    89821 cgctatgtac ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact
    89881 gcgttttatc accaacgcct ttacccgtat gcgtttttgc ttcccgaacg gtcaactgga
    89941 tatgtacagc aaagaatcgc cggaagaagc ccctgcccca ctgaaaccgt ggtttgcgat
    90001 tcctggccct gtcgctgaag aatacagcat cgcctttggt cactgggcat cgctggaagg
    90061 caaaggtacg ccggaaggta tttacgcgct ggataccggc tgctgctggg gtgggtcatt
    90121 aacctgcctg cgctgggaag ataaacagta ttttgtccag ccgtcgaacc ggcataagga
    90181 tttgggcgaa gcggcggcgt cttaaacaca gcctgataca ggaaggccgg ataagacgcg
    90241 accggcgtcg catccggcac tagccgtaaa ttctatacaa acttaccgcc gctccagaat
    90301 ctcaaagcaa tagctgtgag agttctgcgc atcggcgtcg tggaattcgc tgaataccga
    90361 ttcccagtca tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat
    90421 atgcgtcaga tacagttttt gcgcttttgg caggaactgc tcataaacgc gaccgccgcc
    90481 aatcaccatg atttctggta cgtcaccaca cgccgcaatg gcttcatcca ccgatttcac
    90541 ccacgttacg cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg
    90601 caacggacga ccgattgatt cccaagtatg gcggcccata atcacgggtt tatttaaggt
    90661 gttgcgttta aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc
    90721 gataacgcga tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa
    90781 aaattgtcgc cactatacgt aaagcgtaaa ccgtcgtcga ctggtgcgag gatgatgttg
    90841 aggaaaattt tatattctgc tggcgagtcc acgctctccc cctggactcg ccgcattaca
    90901 atgaaacaaa aacaaacagt tagctgtaaa gtgtgattta cgtcactctt tattaagatg
    90961 agggtttcgt ttccggttca tccgccatgt tgccggtatg tttaccttct tctgttccct
    91021 gccagccatg tcgttgaatt aatgacagat gttcgcggtc ctcggtgatg atctcactta
    91081 acatcgcgct ggtgcgttta tagaccgccg cgcgggcttt ggtgtcattc tcaaccattg
    91141 ccatctcttc caccatctga atattaaagc ggcggaacac atcggcacgt tctcgcgctt
    91201 catacggccc cagacctaaa ctttccagcg ccagacgccc ggttttcagc gcaccttcaa
    91261 aggtttcacg ctccggcttt tcaaggcctg cctgacgcaa acgaatgtag tgatcgacat
    91321 cgcgggcgcg ggcaataatc tgcaaatgcg ggaaatgttc tttcaccatc tccgtcagtt
    91381 gcaggttggt ttgcggatcg tcaatagcgt taatcagcac ttccgctttc gccgctccgg
    91441 cagattccag taaatccatc cgcgtggcat cgccataaaa cactttcata ccaaatttac
    91501 gcagcgtttc gatatggtcc ggatcgtgat cgagcaccac cattttcacc ccgctggaga
    91561 gcagtaaacg tccggtaatc tgtccaaaac gaccgaatcc ggcgataatc acgcgcggct
    91621 gttcttcatc gatctcatcg gcttcacgcg cctcctccgt agaagattgc tcaaggcgat
    91681 tgaggatcac cagcagaatc ggcgttgctg ccatcgacag cgccaccgcc agggtcagcg
    91741 atttcgccca ctccggctcc agcacattcg ccatctgcgc cgcaccaaat accacaaagg
    91801 caaactcact gccctgccct aacaacaccg caaaccagcg acgctgttta tttggcacct
    91861 gcaacggtcg ggcaatcagc cacagcatgg cgattttgat gatgaggaaa ccgagcagca
    91921 aaatgacaat gcgcaatggg ttttcaatca gcgtgccaaa gtctatcgac atgccaacac
    91981 cgatgaaaaa cagccccaac agcaaacctt taaacggttc gatatcgctt tccaacgcat
    92041 gacggtattc cgagcttgcc agcagtacgc ccgccagaaa cgcgcccatc gccatcgaca
    92101 agccgacctc ttccagcagc aaaccaaagc caaacacaag gaataacgcc acgacactaa
    92161 acacttcccg caagccagag cgggcaacaa aacgcagcgc cggacgcgta acatagcgcc
    92221 ccagcaatac caccagtacc agcgcacccg ccacttttaa cgccgagaga gcaaatgcgc
    92281 ccatcgtcgt cgaggcactg ctcgccgcca gtagcgggat catcgccacc agcgggattg
    92341 ccgcgatatc ctggaacagc agcaccgcaa aggcactgcg gcccatttgc gtcaccatca
    92401 gattgcgttc attcatcgcc tgcatggcaa tcgccgttga tgagagcgcc agcgtcatac
    92461 cgatcaattc cgcgacctgc cagcgcagcc caagtaacat acagaacagc cccagcaggc
    92521 caccgcaaat caccatctgc aatgcgccac cgccgaacac tgccgcacgc agcttccaca
    92581 gcctttgtgg atcgagttcg aggcgataat aaacagcatc agcaccaccc caatctcggc
    92641 aaagtgcaga atagattcgg catcggtcac cagtcgcagc ccccacgggc caataatgca
    92701 gccggcgatc aggtagccaa gtaccgatcc cagaccaaga cgtaccgcaa tgggtacaat
    92761 cagcgccgcc gatccgaggt agatcagcgc ctgaaccagc gtatgactat ccatgatggg
    92821 cctcctgcca ttccagcaga cgttgcttat agtggcgcgc ctgcccttcg agggtttcgt
    92881 cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag ccagttcagc ccgcagtaga
    92941 ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc cggatgcgca ccaatttcaa
    93001 aatggctttc cccgccgccg gtcgtcaccg cccacagcaa atgtttgcca tgcagcgccg
    93061 tgccgccgtg accgtaagcc cagccgtgcg acaaaacttt atcgatccaa agtttaagaa
    93121 gcggaggaat gctgtaccac tgcatcgggt gctgccagac gatcagatcg gcgcgagaga
    93181 gcgcctcctg ctcggcggca atatcgatat tgaagtcagg atagagttga taaagagagc
    93241 gaatttcgac gccttccagc gtccttgcct gttcaagcat ccgtttattc gcatgggaat
    93301 gatgcggata cggatgcgca taaatgatga ggatcatagt tattagcccg cgtgtttacg
    93361 cctttgtttt cccacaaagt gtagtcatat attcacgcgg cttatagtca ataatattga
    93421 ttcagaaaag agagaaatct gaacaaataa tcccattctc tgcccgcgga tcgtcatatt
    93481 gctggtctgc gccagtgaca ttcctcgagt ctccggggca aacgctacgg aaatcaacaa
    93541 gccaaacagc gagatacccg cccccattag catcgtgtta ctgataccgt aattattgat
    93601 aaatatcggc agtgcccagg tcgaaacaat ggtgccaata cgacttaagg acataatcac
    93661 gcccacggca gaggcgcgga tatccgtcgg gaagagttca ttaggataga gccactgcaa
    93721 attacccggc ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat
    93781 ccccatatcc gggattagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc
    93841 aatcaacaat ggacgccgtc cggcagtgtt tagccacagc atcggcggaa tacagccgag
    93901 cataaagaac aggctaatca ccacattccc tagtgccgcg tttttgccaa cccccaatcc
    93961 caacgaacca acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca
    94021 ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg
    94081 aaaacgggtt tgctgcggct gctcttcatc gaaagccacc ggttcgccaa acagttttat
    94141 catcatctct tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattctgg
    94201 cagttcgaat cgaccaatca aaatcaacaa acaagggatc gccgcgctac ccagcatcca
    94261 gcgccaacca ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc
    94321 gacataccac atcgcggcaa taaagctgat ggaaaacgcc cgctgacggg tactggagaa
    94381 ctcggtgatc atcgaagtgg cgatgggata atctgcaccg atgacaatgc cgatgagtac
    94441 ccgcatcacc aacagttcga cgggggatga aacacacatc gtcgccaccg atatcacgcc
    94501 gatggcgatg atatcaatga ggaacatttt gcgccgtccg actttatcgg agatatagcc
    94561 aaacagcgat gtgccaacga acagcccggc gagcgttccc gcgcccagca agccaatcca
    94621 gtcagcgtcc agtttcagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag
    94681 tacataacca tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaataga
    94741 ggagaatttg agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc
    94801 aatgagagtc agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac
    94861 caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga ccccagcccc aaaatgcgac
    94921 aggtgccgca ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct
    94981 tgtacagacc tgcggggcac gctttcacca gcagctccag cacctgttta tcagcatcgg
    95041 ccttcacaac aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga
    95101 cgtccacatt gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag
    95161 attgatgaag cccactttct tgccgtggcg gaggattttc ttgcgcatca gttccggcgc
    95221 actgccgtca atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct
    95281 aaacatgcgt gggttatcaa ggaacgccgg tagtttctgg tacatgcgca tatcgcgcag
    95341 cgggccactc tccagatgct gacgatattc cgcgagtttt tgcttactga aatcgtcgct
    95401 tttcatcgct gaaagcacgg tttttgctgc agcttccccg gcggcaatcg ccagatccat
    95461 accgcgaatg gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac
    95521 gccgtcaccg accagctccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga
    95581 atattccacc agcttgccgc ccgcgatcag cggtgcaacg gccggatgct gtttgaaatc
    95641 ttccagcatt tgcggcaccg attttttcgc gtcatgcaga tgatgcagac cacaaaccag
    95701 ccccagcgac agggtatttt cactggtata aaggaagccg ccgcccatca ggccatcggt
    95761 gggtgatccc gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc
    95821 gattaccgac ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt
    95881 aacgcgtttt gccatcccca acttttcggc aaggatggag ttcaccccat cagcaaggat
    95941 caccgttttc gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg
    96001 ctgtacgagg ttatcgacgc ggatcccggt aattaactgc gcgctcgctt cttcggcctg
    96061 ctccatcaac caggcatcaa atttactgcg caaaacggag taagaacgct gggatggcga
    96121 ggtttcgtca ccgttgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag
    96181 tttttcatgg gtgatcaggc gttctacggg ggcggaatcg gcgaaacctg gaataatgtg
    96241 ttccagactg tgggcataga gacgcccgcc agtaacgttc ttggcacctg cggaattgcc
    96301 acgctcgata actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc
    96361 ggcaagccct gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctccctg
    96421 tcagcggcag agccgtaatg cagccctgcc agagtggatc aacacgctaa agctgcggtc
    96481 agtgccggaa ggatcttcac ggcatcgcca acaatgccgt aatccgcgta ctggaagatc
    96541 ggcgcatttt tatctttatt gatggcgaaa atggtttgcg acgcgttagc gccaaccatg
    96601 tgctggatct gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg
    96661 gagataccga catagcgttc gtgctccatc catttttcgt tttctgccac cggacgagaa
    96721 caggccaact ccgcacctat cgccttgcaa agctgttctg ccagcgcaat gttctctttg
    96781 ctgccaatgc cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg
    96841 ctctggcgcg cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg
    96901 gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg
    96961 atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accgccgtaa
    97021 accatgtgtt tcactgttgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac
    97081 accgccgctt taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt
    97141 ggcaacagca ccagaccgtc agcgccgtgc tggcgaatag tgtcagccat gacaccggcg
    97201 taatcttcga tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg
    97261 agctggattg cctgtgcgcc gtcggcatca ttgaggacaa aggcattgat ttgattagct
    97321 aaagcctgcg caccgttcat cagttccggc agacgagagg gggtatcgct gaatacccag
    97381 acttgagaaa acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag
    97441 caaatgcggc gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgctcgcgct
    97501 gtttcggcgc ggcaacctgt tgttcagacc aggcgtccac ggcgttaaaa ccaatatccg
    97561 ccgccgacca tacctggact ggctttttcg ccgcgccgag aatggctttc atcgaaggaa
    97621 tttgtgggga gttgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg
    97681 tttcggtttc atcttccagt tcgcgctcaa cggtgagggt atctgccgtc agggagataa
    97741 ttttgctgac gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct
    97801 gctgggcata aaggtcggaa gaaccatcgc cacagaggat cagatcaaag cctgctttct
    97861 gggcggctgc agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat
    97921 caatcaccac aatcagttca tccgggccgc gcgatagcac atctttacgc cctttggcgt
    97981 tggtcagggc tttaccgccc acacttaagg ctgtcacctg cgcctctgct gcctgttgct
    98041 ttagctggca agccgcttca atagcgttga gatcgtattg gcttattttg gcatcggctt
    98101 tgctgaagtc taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc
    98161 acttatagca agtaataatc ttcattgcat ctccagaaat catgaaggaa acgtcgttca
    98221 tatcggtggt gttaattaca gatatcacgc cctttaattt ggaaataaaa ctttaatcac
    98281 caatattgaa aatgtcacac gcagataaat ccaactttca atattgtttt atagatcacc
    98341 aatattgaaa gtcagatgct atttttttac ccacaaaaga acataacaaa ctgattaata
    98401 accaaaatta acggaaaacg tcctctattc acccgaaaca aaaatgtgac aacaatcaca
    98461 gaatacagct tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata
    98521 ataattatat taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt
    98581 ttcacagtgg aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt
    98641 tttctttccg ccattaataa tcgtcggcat actttgttgg cttacagtca gagatctgga
    98701 tgcggcgaat gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc
    98761 atttgaatgg tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta
    98821 tgccaaaaag cgtttaggta acgaaccgcc agaatttagc accgccagtt ggatctttat
    98881 gatgttcgcc tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta
    98941 ctacatctcc accccgccgt ttggcttaga accgaactcg acaggagcga aagagttggg
    99001 gctggcttac agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc
    99061 agtcgccttc gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac
    99121 actggtgccg ctggtaggtg aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa
    99181 cttctatctc gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct
    99241 ggtgaccgag tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat
    99301 catcattacc tgctggatta tcctcaacgc catttgcgtc gcttgcggtc tgcaaaaagg
    99361 ggtacgtatc gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttggatgtt
    99421 catcgtcagc ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct
    99481 gatgtatctg ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca
    99541 gggctggacc gtgttctact gggcatggtg ggtgatttac gccatccaga tgagtatctt
    99601 cctcgcccgc atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg
    99661 gctgacagca tccacctgga tcctgtggac cgtgctcggt agtaacactc tgctgttgat
    99721 ggataaaaac atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat
    99781 catcgaaacc tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct
    99841 ctgctttatt gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtctac
    99901 ttgccgcgaa gtacgcgatg gtgaagaacc accgctgctg gtgcgtatcg gttggtcaat
    99961 tctggttggc attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac
   100021 cgccattatc gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt
   100081 tattaaagac gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg
   100141 aaagatggat tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact
   100201 gatggccagc gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc
   100261 ggaacgtttt gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga
   100321 gcacggtggt ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg
   100381 tctgggggca ccaacctatg tactgtacca gctgccgggc gggttcaata ccttcctgcg
   100441 cgaaggcaca caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat
   100501 gtggaactca gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac
   100561 ttatacccgt agaaatggta agatttatct taatggtagt aagtgtttta ttaccagtag
   100621 cgcctacacc ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt
   100681 ctacaccgaa tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgagaa
   100741 gctcggtctg cgtatggata gctgctgtga aatcaccttt gacgatgtgg aactggacga
   100801 gaaagacatg ttcggtcggg aaggtaacgg ctttaaccgc gtcaaagaag agttcgacca
   100861 tgaacgtttc ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc
   100921 tgcgcgctac gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat
   100981 tcaggaaaaa ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga
   101041 agcagcgtgg aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata
   101101 cttctgcgcc aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt
   101161 cgggattgcg ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt
   101221 ttccggagga tctgacgaaa tgcagatcct gacgctgggt cgtgcggtgc tgaagcaata
   101281 ccgctaagtc cgtgcgctgc ctgatgcgac gctgacgcgt cttatcaggc ctacgggagc
   101341 acgaatgtag gccggataag gcgtttacgc cgcatccggc agtcatgcgc tacataaatt
   101401 tttcaggagc taataatgga tcatctaccc atgccgaaat tcggaccgct ggccggattg
   101461 cgcgttgtct tctccggtat cgaaatcgcc ggaccgtttg ccggacaaat gttcgcagaa
   101521 tggggcgcgg aagttatttg gatcgagaac gtcgcctagg ccgacaccat tcgcgttcaa
   101581 ccgaactacc cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa
   101641 gatgaaggcc gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc
   101701 agtaaaggtc cggcctttgc ccgtcgtagc attaccgatg aagtactgtg gcagcacaac
   101761 ccgaaactgg ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc
   101821 aatcttccgg cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt
   101881 gatgttgacc agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc
   101941 gccaccacgg cggcgctggc agcactgcat aaagtgcgtg aaaccggtaa aggcgaaagt
   102001 atcgacatcg ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac
   102061 ttcaacggcg gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt
   102121 tgcggtctgt ataaatgtgc cgacggctac atcgtgatgg aactggtggg cattacccaa
   102181 attgaagagt gctttaaaga tattggcctc gcacatctac tcggtacgcc ggaaatcccg
   102241 gaaggcactc agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa
   102301 ctcgatgcct ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg
   102361 aatatcgcct gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt
   102421 gcccgtgaat caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac
   102481 atcatgccga aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc
   102541 atggacacgg ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg
   102601 gtcagcaaag gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga
   102661 ctagcgcccg cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg
   102721 cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc
   102781 gctgatttgt gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca
   102841 ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcaacaaggt
   102901 tgcactacat ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat
   102961 tggcgcgatt atggtgccga ttaacgcccg cctgttacgc gaggaaagcg agtggatctt
   103021 gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta tgtatcaaca
   103081 gattcagcag gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc
   103141 cgctgatgat ggagtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg
   103201 ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac
   103261 ctcccgaccg aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc
   103321 cgcctggcag tgtgcactgc gtgacgatga cgtctacatg acggtaatgc ctgcgtttca
   103381 tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct
   103441 ggtcgagaaa tacagcgccc gcgccttctg gggacaggcg cagaagtacc gcgccaccat
   103501 taccgaatgt attccgatga tgatccgtac gttgatggtg cagccgcctt cagcgaacga
   103561 tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga
   103621 cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat
   103681 tgtgggcatt atcggcgatc accctagcga taaacgacgc tggccgtcga ttggtcgggc
   103741 ggggttttgc tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga
   103801 gatcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct
   103861 caacccgaaa gccactgcga aagtgctgga agccgacggc tggctacata ccggcgatac
   103921 cggataccgc gacgaagaag gcttttttta tttcgtcgat cgccgctgca atatgatcaa
   103981 acgcggtggc gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccgaa
   104041 aattcaggac atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc
   104101 atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaattttttc gcttctgcga
   104161 acaaaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg
   104221 taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacggc agggaactac
   104281 cctgccagct aataatcacg ccctaaaaca atatgcattt tttgcaggga tgttgtcgtc
   104341 cctaaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg
   104401 atcaattctg gaaattaccc ttgatcgccc aaaagcgaat gctattgatg caaaaaccag
   104461 ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat
   104521 tattaccggt gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga
   104581 aggcgaagca ccggatgctg actttggccc gggtggtttt gcgggattaa ccgaaatttt
   104641 caatctcgac aaaccagtta tcgcagctgt gaacggctac gcctttggcg gcggtttcga
   104701 actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga
   104761 agccaaactg ggcatcgttc ctgacagcgg cggtgtgctg cgtctgccga agatcctgcc
   104821 gcctaccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct
   104881 gcgttggggg atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga
   104941 actggctcag cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatcta
   105001 ccgcaccacc agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt
   105061 gaaacactat ccatcggttc tgcattcgga agatgccatt gaagggccgc tggcgtttgc
   105121 cgagaagcgc gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag
   105181 gggttaattc cggtggttca cccgacggcg tttgtccatc ccagtgccgt cttgattggc
   105241 gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg tggtgactac
   105301 gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat gcatggctac
   105361 tgcgacactg acaccatcgt tggggaaaac ggccatatcg ggcatggagc gatcctgcat
   105421 ggttgtgtga ttggtcgcga tgcattggtc gggatgaaca gcgtaattat ggatggcgcg
   105481 gtcattggcg aagagagcat tgttgccgca atgagctttg tcaaagcagg ctttagtggc
   105541 gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagtgt tagtgacgac
   105601 gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat
   105661 gcatcgttac atgaaacgca gccactgagg caaatggagg aaaatcgccc ccgtttgcag
   105721 gggacgacgg atgtgacgcc taaacggtga tcttgccgga tgcgacctgg ttgctggata
   105781 agatgctaca acatcgtatc tggcaagcag gacgcccggt atcaacgaga taaaattaac
   105841 gacgcatact ctttgacaac atcatctgcc acttctgctc gcggttaagt tccgtgagcg
   105901 gaacggcagg aatacgcggt gtctttctta cgccgactaa tttttcccga ctgttattgc
   105961 gcaggcgcgc gtaaatttgc tcatcgatat cgaccacttt aaccagtcgc tgacactggc
   106021 atccccgccc ttccagctta ttagggatca tcttaacttc gcagcttatt tctgtgactt
   106081 ccgacagaat ataagtcagg gtattaaccg ccttgctgtg ttcaatatcg aaatgaatag
   106141 agatctctct tgctatcacc caccgatttt cagccatcat ccattcggcg attaacaagt
   106201 agagtagttt ttcaacatat ccttcacaca tacgaactcc agtttcacaa attaaaagtc
   106261 tggctatgac tcgttttgaa gcaattaaac aaggccatat taaaattgtg gatatatcga
   106321 tagtatgcaa cttcaccgtc gataagtgtg agctaaatcc tgcttatgtc atcaaaaata
   106381 tcgattcccc aaaagattta cttaatgggc aaaaaaaaac ggtcctcatc agagaaccgt
   106441 attagttatt cgatcaaatt agcggatgaa aaatatccgc cacgacacgc tattatttga
   106501 tctgtgcgtg catttcctgc accgaaatta ctttttcagt cgcatcggca ttcagcgcca
   106561 tcgcggtagc gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac
   106621 tgcgacggat cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt
   106681 aggtatattc gccattcttg atacggtcct gaatgtgcgg acggccttca tgcaccttgt
   106741 ttaccagacg cggattgata cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat
   106801 ccagctcgaa gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc
   106861 cttcgcgcac ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct
   106921 gcgctttggc gaacgcttca gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc
   106981 gcatttctgg ccctaacagc gggtcaacgc ccgggaattt attgaacggc agcaccactt
   107041 ctttcaccga gtagtacggc ggaataactt ctttggttac gccctgctca gccagcgatt
   107101 tgccagccat cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga
   107161 acggaacagt acgcgccgca cgcgggttta cttcaatcag gtaaacttcg ttgtttttca
   107221 ccgcaaactg cacgttcatc agaccgcgca cctgcaattc gaaagccagt ttctgcacct
   107281 gctggcgcat cacatcctga atttcctgac ttaaggtgta cgctggcagc gaacatgcgg
   107341 agtcaccgga gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca
   107401 tttcgccgtc gcagatggca tccacgtcaa cttctactgc gtcatcaagg aaatgatcca
   107461 gcaacactgg cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag
   107521 cttcgtcata gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgcaccacca
   107581 gcgggtagcc aatctctttt gccttctcaa ctgccatttc aatagcagta acggtggcgt
   107641 tcgccggttg tttcagtttc agacggtcaa ccgcatgctg gaagcgttca cggtcttccg
   107701 cacggtcgat agcatccggg ctggtgccga taaccggtac gccagcggct tccagtgcac
   107761 gcgccagttt cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa
   107821 tacgcacgat ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag
   107881 tgtcgtagtc ggtagagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt
   107941 cttcacgcag cgccagcgag gcgtgtacgc agcagtagtc gaattcgata ccctgaccga
   108001 tacggtttgg accgccgcca agcaccatga ttttttcacg gtcggtagac ggattcgctt
   108061 cgcactcttc ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac
   108121 aggtatccac gcgcttgtag accgggtgca ggtcatattg gtcacgcagc ttacggattt
   108181 ccgcttcgcg tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca
   108241 gctggcgcag gaattcagcg ttcaggccag tgatgcccac ttccgccact ttctcttcca
   108301 gacgcaccag ttcttcaatc tgtaccagga accagcggtc aatgttagtc aggttgaaga
   108361 cgccgtccac agacaggccc gcgcggaaag catcggcgat gtaccagata cgctctgcgc
   108421 ctgcgtcttt cagttcgcga cggattttgg ttaacgcttc cgggtcatct aggctcactt
   108481 tcgggtcgaa gccagttgcg cccacttcca ggccgcgcag cgctttttgc agggattcct
   108541 gctgcgtgcg accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt
   108601 cgttagcacc ggcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga
   108661 tggacggctc gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga
   108721 gggtgtaacc caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg
   108781 acgccagcgc cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt
   108841 ttttcgggtt caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca
   108901 gcaccgccat cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg
   108961 gcgcgacagt gatggagtca ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc
   109021 agacgatgat gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc
   109081 cgatcagcga ctcatcaatc agcaactctt tggtcggaga gagatccaga ccgcgggcgc
   109141 aaatttcttc aaactcttca cggttataag cgataccgcc gccgctaccg cccatggtaa
   109201 aggatgggcg aataatgcac gggaagccca cgtcagcggc aacagccagc gcttcttcca
   109261 tcgtgtgtgc gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga
   109321 aacgacggcg gtcttctgct ttatcaatcg catcggcagt ggcaccaatc atggtgacac
   109381 cgaactcttc caacacgccc tgacgttcca gctccagcgc gcagttcagc gccgtctgac
   109441 cgcccatggt tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtaccactt
   109501 cccagtgaat cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg
   109561 ccgggttgga gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt
   109621 gcgcgccaga gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa
   109681 tcaggatact ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc
   109741 ggttttacgg tactgctcaa ttaacttgat aaagtggtcg aacaacggcg cggcgtcgtg
   109801 tggaccaggg ctggcttcag ggtgaccctg gaagctgaac gccggtttat cgatgcgatg
   109861 aatgccctgt aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa
   109921 tgttgcttca tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc
   109981 cacatcttta accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc
   110041 accgctcgcc agcgccagca gctgatgacc gagacagatg ccgaataccg gaatatcggt
   110101 ttcgaggaac ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc
   110161 gttggagagg aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc
   110221 cggaacgatg gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc
   110281 accaaaatca taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag
   110341 gccgccggtc aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc
   110401 ttttgccaga tccatgccgt tcagacctag gaacgcgcgg gctttttcta acgccagcgc
   110461 cgcatccggg ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa
   110521 acgcgtcagc ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta
   110581 agaagagagg tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac
   110641 cagaccttgt gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc
   110701 aatatgggga taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc
   110761 ttgataaccg gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc
   110821 tatggcccga ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa
   110881 aacaccctcc agagaatatt cactcacttt atttgcatat taattcacag tgatgacgta
   110941 aatcaatgca aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg
   111001 ggcgctaaaa gtcaacttaa tggcaggatt ttttatatta ttttgtggct catgcatgtt
   111061 ttacaggcat taagcgacaa aaacatcatc taacctgttc tggaaaacgc ttgcgcagca
   111121 agaagattgc atgaaataaa aacgagcagg aaaaagtgga ccaaatggtc aaaatttaca
   111181 ttaaaacaac aaaaatagaa gacgaaacat gtaatttttg gcagttatag aaaatttaaa
   111241 agcataacac caaaaataaa agggctaaaa attaaagccc tttataatta ataaattaaa
   111301 tgtgttactt ttgcatcata ataaacattt tgtggttaca aattattgag atcaagcaca
   111361 tctcgcatat caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgca
   111421 ccgttagcaa atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata
   111481 tcggcaaaca tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt ggcaaaacca
   111541 atggtaccag gcacacgttc accggtatgg ccttcacgac tgtagaccgc gcaatctttc
   111601 agatctttat caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc
   111661 gcatcaactt tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc
   111721 actttggctg ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg
   111781 gcaaagacaa tcgcaatatc ggcggcggcg tcacgaattg cctgtttacc ggcttcgtca
   111841 aaccccgtag tgccgatcac catccctttg ccatgctgcc gacaaaaagc gagatggttc
   111901 agcgtacctt ccggacgggt aaaatcgata aacacatcaa aatcatcttt tatcgcatca
   111961 agactgcttt gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg
   112021 cccagtaaag aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc
   112081 agtgccgcct gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg
   112141 atgtttgcat catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta
   112201 accagcgcat cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca
   112261 gggagggata tcagtgaaag gcatgagtcg atgaatgact gcatgccgat aacatgtgac
   112321 aggttacggc accagagcca gcacctcagc cacccactgt tggaagcctt ttacattcag
   112381 atccaatgcc acctgtacat tggctggttt gcccaggcaa ccgtcgatat caaccaccgt
   112441 cgtgcctgag gtaaattcgc cttgagtttc taccgcgaca aaacacggct tgagggtgaa
   112501 taagtccggg cgcaccagcc aggcgatggc gcagagatcg tgcattcgca gaccgctttg
   112561 catactgccg ctacggtagt ggctaaacag ggcgtggagc atttttccgg tgcgattcaa
   112621 ctccggtaaa gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc
   112681 gcacatgaca atttcgatgc cgctacggaa gacacaggca gccgcttctg gatcggcagc
   112741 gatattaaac tcggcatttg gtgtacagtt gccgcgacca gcagaaccgc ccatgatcac
   112801 cagacgacga atatacggtt tacattccgg gcattgtaaa agtaacagcg caatattggt
   112861 taacgggccg atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat
   112921 cgccagaaac gccggtatcc cgagtggctt tcggttgtgc tcaacaaagt cgtagccagc
   112981 cattcccgat tcgccgtgca cagatgctgc atcacgcggt gcgcgtacca gcggcacagc
   113041 ggctccctgg gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcattgcg
   113101 ggtagttttc tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc
   113161 gggggcaaaa atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag
   113221 gaagataggt aaacgcatgt tttcttcata aaaaatgccg gtaacaagac cggcattttc
   113281 gcataactta ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc
   113341 ggcacttcga aaacaatatt ttcttcacgg ccttccagcg gaatggcttc accaccaccc
   113401 aactgctgca aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca
   113461 gtcacgccga cgcatttaac ctctttcacc cactcttcct ggatatcttt cgcatcgtca
   113521 atcaaaaacg cgtgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag
   113581 gagtttttcg aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct
   113641 tcctgacggt tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg
   113701 aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtttgg
   113761 gtcataaagg agagcttctc ttcgtttttg accgtcagtt tccacacatc gtctggcgat
   113821 tcgaccagat acattccccc ttcggggttg ctgtactgcc ccatcgtccc ttccacttcc
   113881 gggtgcccgg cgtggccgat gagaatagat tcttcgccac ggcgactggc gcgggcaact
   113941 tccatatgca ctttggtcac cagcggacag gtggcatcaa acaccgtcaa atcgcggctt
   114001 ttcgcttcgt tacgaaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg
   114061 tccggcactt cgctaatctg ctcaataaag atagccccac gctcgcgcaa gctatcgacc
   114121 acgtagcggt tatgcaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc
   114181 gcgttttcaa caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc
   114241 aacaggatct gcatgttacg cctccagtgc cggatcgatt tccagcactt caatatcaaa
   114301 atgaacggtc tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat
   114361 ttcgcggatc acaccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc
   114421 aatttctggc tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg
   114481 tgacggcacg ccaaacgccg catctggctc cagcgagaag gtggttttgt cgcccacttt
   114541 cagccccagc aggtgttgct ccagcccttc agaaagagaa gcatcgccca ggcggaacag
   114601 cgccggttta ccgttgttgc gagtagactc ggcggtggtg ccatcgtcga gttttagcgt
   114661 gaagtgcacc aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta
   114721 tttttgagga tttttgccgg atgcggccta cgatttcgta gcaatctgta ggccgcatcc
   114781 ggcagggttt attattgttt tttcgctttg gaaggtaaaa acccttccag cacgatcagt
   114841 gccgcaccga cacaaatggc agtatcggca aggttgaagg tggcgaagtg ccagtcgccg
   114901 acgtagaagt cgatcatatc gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc
   114961 agcgcgccgc caataatcag cgcgtaagcg atattgttta gcttctgcgt ggccttcgag
   115021 cgatacatca tcactgccag gagcacgcta ataccaatcg caataccggc aaagaaccaa
   115081 cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa
   115141 tgcagattaa gcgacgggaa cagcgggacc gtatccccca gagcaaagtt ctggaggatc
   115201 aggtatttgc tgcccagatc gataatcagc acgactacca ccagccacag ccagcgtagc
   115261 cctgttgaac agatcgattg actcatcagg caaacttacg tttttcaccg tcaccggcga
   115321 cgttgctgac acagcggccg cagatttctg cgtgttccgc caccttgccg acatcctggg
   115381 tgtagtgcca gcagcgtggg cacttctcac cttcggcttt actcaacgcg actttcagcc
   115441 ctttgagtac ttcgctctgc tgagcatcag caggtgcgtc gttatagtct gcaacggtag
   115501 cgccggaggt caacaggaca aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca
   115561 gttccggttc tacatacaag gttactgccg cttccagcga gccacccact ttcttgtcgg
   115621 cacgcgcttg ctcaatgact ttgttcactt cgccacgcac tttcaacagc tcgtcccaga
   115681 acgcatcgtt cattgcttca ctgtctgcca gaccaaacag gccttcgtac cactcaccgg
   115741 tgaagacgta tttttcacgt tcgcccggca gatagcccca cacttcatca gcggtgaagg
   115801 agaggattgg tgccatccag cgcaccagcg cttcagcgat gtgatacagc gcagtctggc
   115861 agctacgacg cgccacgcta tccgctttgg cggtgtactg acggtctttg atgatgtcga
   115921 ggtagaagga acccatctca acggagcaga agcgcatcag acgctgtacc acttcgtgga
   115981 aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc cgctttcgca caacctacgg
   116041 cccagcgatc cagtaccacc atctcttccg gtttcaccat atcttttgct ggatcaaaac
   116101 cattcaggtt tgccagcagg aagcgcgcgg tgttacggat acgacgatag ctatcggcag
   116161 cacgtttcag gatctcgtca gaaacggcca tttcaccggt gtagtcggtt gatgccaccc
   116221 acagacgcag aatatccgcg cccagtttgt tcatcacatc ctgcggcgaa acggtgttgc
   116281 cgatggattt agacatcttg cgaccctgac catccacggt aaagccgtgg gtcagtacct
   116341 gacgatacgg cgctttgcct ttcatcgcgg tggagatcat tagggaagac atgaaccaac
   116401 cacggtgctg gtcagaacct tccagataca tgtccgctgc gtgaccggca aattccggac
   116461 gcacgtcaac aacagaagag tgggtagatc cggagtcaaa ccatacgtcg agagtatccg
   116521 gcactttcac atactggtca gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc
   116581 acgcctggat gccgtcaact tcaacgcgtt tagccacttc ttccatcagt tcgagggtac
   116641 gcggatgcag ctcttccgtg tctttgtgca cgaacagtga catcggtacg ccccaggtgc
   116701 gctgacggga gatacaccag tcaggacggt tagcaaccat cgactcgata cgcgcctggc
   116761 cccagtccgg gatccactgc acgcctttga tctctttcag tgactgcgca cgcagacctt
   116821 tctgatccat gctgacgaac cactgcggtg tcgcgcggaa gatgatcggc gttttgtgac
   116881 gccagcagca cggatagctg tgctgcattt tctcaacgtg cagcagcgcg cctttttcct
   116941 gcagcagcgc aacgacgatg tcgttcgctt tgaagacgtt cacgccatcc agcgtcggat
   117001 aagtgcccgg cagataagtg ccgtccgggc caaccgggtt agcggtttcc aggccgtatt
   117061 tctgaccgat cacatagtcg tccgggccgt ggccaggcgc ggtgtgaacg gcaccggtac
   117121 cggcatccag ggtaacgtga tcgccgagga ttgccggaac gtcgaagccc ataaacggat
   117181 gggtaaagcg cagcagctca agctctgcac cttttaccgt gccgagaatg gtgtaatcgg
   117241 tcacgccgat acgctgcatt acgctttcaa ccagatcttt cgccagaatc acggcctgac
   117301 cgtcgatctg caccagcgca tagtcgaaat caggtgcaat agagattgcg cggttcgcag
   117361 gcagagtcca cggcgtagtg gtccagatca ccagcgagat tgggccgtta acgttgctta
   117421 cgccaaattt cgctttcagc gcatcctgat cgaccgcctg gaaagcgacg tcaatggacg
   117481 gagaagtttt gtcgtaatac tcaacttccg cttctgccag tgcagaacgg cagtctacgc
   117541 accagtgcac cggcttcgcg cctttgtgca ggtgaccatt gccgatgatt ttgcccagcg
   117601 cgcggatgat gttggcttca gttttgaagt ccatagtcag atacgggtgc gaccagtcgc
   117661 ccagcacgcc cagacggata aagtctttgc gttgaccgtc aacctgggtc gccgcgtatt
   117721 cgcggcactt ggcgcggaac tcggcggcgg tgaatttctc acccggctta ccgtattctt
   117781 gctctacttt cagctcgatc ggcagaccgt ggcagtccca gccaggcaca tacggcgagt
   117841 cataaccgga aagccctttg gacttcacga taatgtcttt cagaatcttg ttaaccgagt
   117901 gaccaatatg aatgctgcca ttcgcataag gagggccatc atgcagaatg aaggtttttt
   117961 tgcctttttt agccgcacgg atgatgccgt acagatcatc atcagtccaa cgcgccagca
   118021 ttcccggttc gcgcttggcg agatcgccac gcatcgggaa ccctgtttcc ggcaaattca
   118081 gggttgattt atagtcactc atcagattct cggttccgtg tttcggtttg attacataac
   118141 aggcttaagc cggttttgtt agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa
   118201 tctgcgcttt cagttcgtcc agcgacgcaa atcgctgctc attgcgtatt tttttacgca
   118261 gcactacttg tatatggcga ccgtaaaggt ccattgcaac atctaacaaa tgcacttcca
   118321 gctgctggcg aataccggca accgttgggc gtgttccgat gtttgccacg ccgggtaacg
   118381 gcttttcacc gaggcccagc acttctaccg cataaacccc tttcaccggg gaaacctgac
   118441 ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga
   118501 ctacacgccc ggagatggca aacgggtgcc ccagtaaact ttctgccaga gccagattgt
   118561 catcagccag cgcctgacgc acggcggtgc tgctgatacg cacaccacct tcgcaaaaag
   118621 tttgcgtact ggtgatatcg aagccgtatt ccatgccagc tttctgtaat aacaagaaat
   118681 cgccttcacg accagcgcca aagcggaaat catcacctac ggcaagaaat tttacgcgca
   118741 aatgcttcac caaaagatcg ctgatgaaat tttgcgcggt taacgccgca aaacgcctgt
   118801 cgaaacgcac gcacagcacg taatcaacgc cacactctgc aaggtaacgc agtttttccc
   118861 gcagccgggt cagtcttgcc ggggctttat cggtagcaaa cagttccagt ggttgaggtt
   118921 caaaaagcat caccatcacc ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta
   118981 acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc aatagtcagc acacaccctt
   119041 cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac
   119101 agtgaaaatc gtccgagtat accttgtaca gcggtcaagg ttaaccggcg attgagtacc
   119161 gaaatcagaa agaagtggct tttcattgcc atggcgcaaa tcacgggaag aaactgaccg
   119221 cctgctgcaa tttttatcgc ggaaaagctg tattcacacc ccgcaagctg gtagaatcct
   119281 gcgccatcac tacgtaacga gtgccggcac attaacggcg cttatttgca caaatccatt
   119341 gacaaaagaa ggctaaaagg gcatattcct cggcctttga attgtccata tagaacacat
   119401 ttgggagttg gaccttggct aatatcaaat cagctaagaa gcgcgccatt cagtctgaaa
   119461 aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg tactttcatc aagaaagtat
   119521 acgcagctat cgaagctggc gacaaagctg ctgcacagaa agcatttaac gaaatgcaac
   119581 cgatcgtgga ccgtcaggct gctaaaggtc tgatccacaa aaacaaagct gcacgtcata
   119641 aggctaacct gactgcacag atcaacaaac tggcttaatc gccaatttgc tgaagctttg
   119701 tgaaaaagcc cgcgcaagcg ggttttttta tgcctgctgc ttttgtgatg cgtgagagaa
   119761 cacactgccg gttgccagat agtcaaaaga gacatgccgt tgtactatct gaactcttcg
   119821 tggcttaaga atcgtagaac catcggctga tacattctga acaagaatat ctactcaatc
   119881 tccagactcc cccacaaaga atatggatat tgtgatacac attgaggtat taaacttaag
   119941 accactctat ttttgaatta aattcctata tactttaacc attcataaat atctcctcat
   120001 tagacc
//
LOCUS       DADLSE010000002        84680 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLSE010000002 DADLSE010000000
VERSION     DADLSE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 84680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.002"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN692_000102"
     CDS             complement(<1..869)
                     /gene="glxR"
                     /locus_tag="JN692_000102"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7262520.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANH"
     gene            complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN692_000103"
     CDS             complement(969..1745)
                     /gene="hyi"
                     /locus_tag="JN692_000103"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7262521.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWIGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN692_000104"
     CDS             complement(1758..3539)
                     /gene="gcl"
                     /locus_tag="JN692_000104"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7262522.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGDIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN692_000105"
     CDS             complement(3629..4444)
                     /gene="allR"
                     /locus_tag="JN692_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7262523.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN692_000106"
     CDS             complement(4522..5004)
                     /gene="allA"
                     /locus_tag="JN692_000106"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7262524.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELSFA
                     "
     gene            5234..6160
                     /gene="allS"
                     /locus_tag="JN692_000107"
     CDS             5234..6160
                     /gene="allS"
                     /locus_tag="JN692_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7262525.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNSQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6229..7323
                     /gene="mnmH"
                     /locus_tag="JN692_000108"
     CDS             6229..7323
                     /gene="mnmH"
                     /locus_tag="JN692_000108"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7262526.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(8034..10448)
                     /locus_tag="JN692_000109"
     CDS             complement(8034..10448)
                     /locus_tag="JN692_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7262527.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(10445..11131)
                     /locus_tag="JN692_000110"
     CDS             complement(10445..11131)
                     /locus_tag="JN692_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7262528.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            11102..11725
                     /gene="tesA"
                     /locus_tag="JN692_000111"
     CDS             11102..11725
                     /gene="tesA"
                     /locus_tag="JN692_000111"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HAY7262529.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            11715..12524
                     /gene="ybbO"
                     /locus_tag="JN692_000112"
     CDS             11715..12524
                     /gene="ybbO"
                     /locus_tag="JN692_000112"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7262530.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            12585..13439
                     /gene="cnoX"
                     /locus_tag="JN692_000113"
     CDS             12585..13439
                     /gene="cnoX"
                     /locus_tag="JN692_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7262531.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPKEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN692_000114"
     CDS             complement(13502..14281)
                     /gene="fetB"
                     /locus_tag="JN692_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7262532.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN692_000115"
     CDS             complement(14268..14945)
                     /gene="fetA"
                     /locus_tag="JN692_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7262533.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            15091..16008
                     /locus_tag="JN692_000116"
     CDS             15091..16008
                     /locus_tag="JN692_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7262534.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            16005..16463
                     /locus_tag="JN692_000117"
     CDS             16005..16463
                     /locus_tag="JN692_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7262535.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(16464..16871)
                     /locus_tag="JN692_000118"
     CDS             complement(16464..16871)
                     /locus_tag="JN692_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7262536.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(16996..18288)
                     /locus_tag="JN692_000119"
     CDS             complement(16996..18288)
                     /locus_tag="JN692_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7262537.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPCNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN692_000120"
     CDS             complement(18291..19223)
                     /gene="glsA"
                     /locus_tag="JN692_000120"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7262538.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            19485..21989
                     /gene="copA"
                     /locus_tag="JN692_000121"
     CDS             19485..21989
                     /gene="copA"
                     /locus_tag="JN692_000121"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7262539.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITRGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(22204..22545)
                     /locus_tag="JN692_000122"
     CDS             complement(22204..22545)
                     /locus_tag="JN692_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7262540.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            22683..23477
                     /locus_tag="JN692_000123"
     CDS             22683..23477
                     /locus_tag="JN692_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262541.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            23681..24160
                     /gene="ybaK"
                     /locus_tag="JN692_000124"
     CDS             23681..24160
                     /gene="ybaK"
                     /locus_tag="JN692_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7262542.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(24197..25849)
                     /locus_tag="JN692_000125"
     CDS             complement(24197..25849)
                     /locus_tag="JN692_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7262543.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEYGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNNPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            26067..27287
                     /locus_tag="JN692_000126"
     CDS             26067..27287
                     /locus_tag="JN692_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262544.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            27525..29201
                     /locus_tag="JN692_000127"
     CDS             27525..29201
                     /locus_tag="JN692_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7262545.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(29334..30638)
                     /locus_tag="JN692_000128"
     CDS             complement(29334..30638)
                     /locus_tag="JN692_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7262546.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPSHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNIPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            30790..31749
                     /gene="aes"
                     /locus_tag="JN692_000129"
     CDS             30790..31749
                     /gene="aes"
                     /locus_tag="JN692_000129"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HAY7262547.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN692_000130"
     CDS             complement(31746..32708)
                     /gene="hemH"
                     /locus_tag="JN692_000130"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7262548.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDEALKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN692_000131"
     CDS             complement(32840..33484)
                     /gene="adk"
                     /locus_tag="JN692_000131"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7262549.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN692_000132"
     CDS             complement(33665..35539)
                     /gene="htpG"
                     /locus_tag="JN692_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7262550.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN692_000133"
     CDS             complement(35649..36254)
                     /gene="recR"
                     /locus_tag="JN692_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7262551.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(36254..36583)
                     /locus_tag="JN692_000134"
     CDS             complement(36254..36583)
                     /locus_tag="JN692_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7262552.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     NINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN692_000135"
     CDS             complement(36636..38561)
                     /gene="dnaX"
                     /locus_tag="JN692_000135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7262553.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN692_000136"
     CDS             complement(38690..39241)
                     /gene="apt"
                     /locus_tag="JN692_000136"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7262554.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(39394..39771)
                     /locus_tag="JN692_000137"
     CDS             complement(39394..39771)
                     /locus_tag="JN692_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7262555.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39841..40368
                     /gene="priC"
                     /locus_tag="JN692_000138"
     CDS             39841..40368
                     /gene="priC"
                     /locus_tag="JN692_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7262556.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            40382..40543
                     /gene="rsmS"
                     /locus_tag="JN692_000139"
     CDS             40382..40543
                     /gene="rsmS"
                     /locus_tag="JN692_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7262557.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN692_000140"
     CDS             complement(40755..44117)
                     /gene="mscK"
                     /locus_tag="JN692_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7262558.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN692_000141"
     CDS             complement(44245..44892)
                     /gene="acrR"
                     /locus_tag="JN692_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7262559.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            45034..46227
                     /gene="acrA"
                     /locus_tag="JN692_000142"
     CDS             45034..46227
                     /gene="acrA"
                     /locus_tag="JN692_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7262560.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            46250..49399
                     /locus_tag="JN692_000143"
     CDS             46250..49399
                     /locus_tag="JN692_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7262561.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFANDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            49945..50319
                     /gene="tomB"
                     /locus_tag="JN692_000144"
     CDS             49945..50319
                     /gene="tomB"
                     /locus_tag="JN692_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7262562.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     LGNRLFRCFVNATKENPASLSC"
     gene            50345..50563
                     /gene="hha"
                     /locus_tag="JN692_000145"
     CDS             50345..50563
                     /gene="hha"
                     /locus_tag="JN692_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7262563.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            50735..51286
                     /gene="maa"
                     /locus_tag="JN692_000146"
     CDS             50735..51286
                     /gene="maa"
                     /locus_tag="JN692_000146"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7262564.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     ALGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            51402..51872
                     /locus_tag="JN692_000147"
     CDS             51402..51872
                     /locus_tag="JN692_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262565.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            52036..53586
                     /gene="pdeB"
                     /locus_tag="JN692_000148"
                     /pseudo
     CDS             52036..53586
                     /gene="pdeB"
                     /locus_tag="JN692_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(53628..53981)
                     /locus_tag="JN692_000149"
     CDS             complement(53628..53981)
                     /locus_tag="JN692_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7262566.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(54101..54197)
                     /gene="ffs"
                     /locus_tag="JN692_000150"
     ncRNA           complement(54101..54197)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN692_000150"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            54360..54671
                     /locus_tag="JN692_000151"
     CDS             54360..54671
                     /locus_tag="JN692_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7262567.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(54702..55274)
                     /locus_tag="JN692_000152"
     CDS             complement(54702..55274)
                     /locus_tag="JN692_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262568.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAINVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            55492..56352
                     /gene="tesB"
                     /locus_tag="JN692_000153"
     CDS             55492..56352
                     /gene="tesB"
                     /locus_tag="JN692_000153"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7262569.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN692_000154"
     CDS             complement(56401..57687)
                     /gene="amtB"
                     /locus_tag="JN692_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7262570.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATEAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN692_000155"
     CDS             complement(57717..58055)
                     /gene="glnK"
                     /locus_tag="JN692_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7262571.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(58236..60017)
                     /locus_tag="JN692_000156"
     CDS             complement(58236..60017)
                     /locus_tag="JN692_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7262572.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(60010..61782)
                     /locus_tag="JN692_000157"
     CDS             complement(60010..61782)
                     /locus_tag="JN692_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7262573.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAVDAKDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFVLKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(61812..62357)
                     /locus_tag="JN692_000158"
     CDS             complement(61812..62357)
                     /locus_tag="JN692_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAY7262574.1"
                     /translation="MVFKPHRINCRDFTFKIELKEKKILRGRAMLDKIDRKLLALLQQ
                     DCTLSLQALAEAVNLTTTPCWKRLKRLEDDGILIGKVALLDPEKIGLGLTAFVLIKTQ
                     HHSSEWYCRFVTVVTEMPEVLGFWRMAGEYDYLMRVQVADMKRYDEFYKRLVNSVPGL
                     SDVTSSFAMEQIKYTTSLPIE"
     gene            complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN692_000159"
     CDS             complement(62424..63242)
                     /gene="cof"
                     /locus_tag="JN692_000159"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7262575.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATYCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            63342..65042
                     /locus_tag="JN692_000160"
     CDS             63342..65042
                     /locus_tag="JN692_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HAY7262576.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPS
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            65107..65802
                     /gene="queC"
                     /locus_tag="JN692_000161"
     CDS             65107..65802
                     /gene="queC"
                     /locus_tag="JN692_000161"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7262577.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(65854..66252)
                     /locus_tag="JN692_000162"
     CDS             complement(65854..66252)
                     /locus_tag="JN692_000162"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7262578.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(66346..66717)
                     /locus_tag="JN692_000163"
     CDS             complement(66346..66717)
                     /locus_tag="JN692_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262579.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN692_000164"
     CDS             complement(66868..68739)
                     /gene="ppiD"
                     /locus_tag="JN692_000164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7262580.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN692_000165"
     CDS             complement(68931..69203)
                     /gene="hupB"
                     /locus_tag="JN692_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7262581.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN692_000166"
     CDS             complement(69412..71766)
                     /gene="lon"
                     /locus_tag="JN692_000166"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7262582.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN692_000167"
     CDS             complement(71954..73228)
                     /gene="clpX"
                     /locus_tag="JN692_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7262583.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN692_000168"
     CDS             complement(73354..73977)
                     /gene="clpP"
                     /locus_tag="JN692_000168"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7262584.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN692_000169"
     CDS             complement(74224..75522)
                     /gene="tig"
                     /locus_tag="JN692_000169"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7262585.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN692_000170"
     CDS             complement(75866..76183)
                     /gene="bolA"
                     /locus_tag="JN692_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7262586.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            76488..77066
                     /locus_tag="JN692_000171"
     CDS             76488..77066
                     /locus_tag="JN692_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7262587.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            77110..78585
                     /gene="ampG"
                     /locus_tag="JN692_000172"
     CDS             77110..78585
                     /gene="ampG"
                     /locus_tag="JN692_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7262588.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            79045..79992
                     /gene="cyoA"
                     /locus_tag="JN692_000173"
                     /pseudo
     CDS             79045..79992
                     /gene="cyoA"
                     /locus_tag="JN692_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            80014..82005
                     /gene="cyoB"
                     /locus_tag="JN692_000174"
     CDS             80014..82005
                     /gene="cyoB"
                     /locus_tag="JN692_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HAY7262589.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGASANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNCDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            81995..82609
                     /locus_tag="JN692_000175"
     CDS             81995..82609
                     /locus_tag="JN692_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HAY7262590.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMKIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            82609..82938
                     /locus_tag="JN692_000176"
     CDS             82609..82938
                     /locus_tag="JN692_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HAY7262591.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            82950..83840
                     /gene="cyoE"
                     /locus_tag="JN692_000177"
     CDS             82950..83840
                     /gene="cyoE"
                     /locus_tag="JN692_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HAY7262592.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            84025..84336
                     /locus_tag="JN692_000178"
     CDS             84025..84336
                     /locus_tag="JN692_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7262593.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSVAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            84320..>84680
                     /locus_tag="JN692_000179"
     CDS             84320..>84680
                     /locus_tag="JN692_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HAY7262594.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLC"
BASE COUNT        19998 a        22507 c        21397 g        20778 t
ORIGIN      
        1 ttatggttag ccattaattc cagcgcctgc actaacgcag agtgatccaa ctggctgcca
       61 ccgtttgccg cacaggtatt aaataactcc tggcaggtcg cagtgtttgg caggttcagc
      121 gcaagtgctt tcgcactttg cagtgccagg ttgagatctt tctggtgcag agcgattttg
      181 aagcccggat taaaggtgcg tttaatcatg cgctcaccgt gaacttccag aatacgtgag
      241 gaagcaaagc cgcccatcag cgcctggcgc acacgtaccg ggtccgcacc ggcttttgaa
      301 gcaaatagca gggcttcaga aaccgcttca atattgagcg ccacaataat ctgatttgcc
      361 actttgcagg tttgaccatc gccgttaccg cccacgaggg tgatattttt accgagcagt
      421 tcaaacagcg gtttaacacg ttcaaatacc gcttcatcac cgccaaccat aatcgacaac
      481 gtcccttcac gcgcaccgat ttcaccgccg gagactggcg catcgagata atcgccgccc
      541 agttcattca cctgacgagc gaaacgctta gtttcaatcg gggaaatgga gctcatatca
      601 acaatggttt tgcccttcag cgaggctttg gtacaaccat tttcaccgaa cagaacttct
      661 tcaacctgag gtgtgtccgg caccataata aaaatgatgt ccgatgcttc cgttacctgg
      721 cgagcagttt caacactgac ggcacccagt gacagtaatt catcagcaac cggtccaatg
      781 gtcgtgacat gtaattgatg accggcacgc gccagattaa tggccatcgg tgtacccata
      841 atgcctaagc caataaatcc cagtttcata attaacctct tttaaatttc gcttttcctg
      901 aattcagaca acacgatccc ctgccaactt agcggcctaa aaaagcattg tctgaatagc
      961 gttacgtttt aacggtacgg atccatccag cgtaaaccag cttccgtggt ggtttggggt
     1021 ttatattcac acccaatcca accgttgtag tcagagtttt caattaccgt aaagagatat
     1081 tcgtaattaa tttctccggt tcccggttcg ccgcgatgcg gattatcagc aatttgcagg
     1141 tgaccaattt tatcggccca ctgagtcatg gtgttggtta attcaccttc catccgctgc
     1201 atgtgataaa tgtcatactg aattttcaaa ttgcagcaac caacatcatc aatcaatttc
     1261 agcgcctgcc gagttccggt gagatggaaa ccaggaatat caaaatggtt aataggttca
     1321 atcagtaata aaatatcttc tttcatcagc atattcgcgg cataacgcag gttttctaca
     1381 agcgttgcgt gaatctgttc actgctgaaa ccagccggcg ttttaccgac cagacagtta
     1441 atttttttat tacccagcgc acgggcataa cgaatcgctg ctgctacgcc atcccgaaac
     1501 tcttcttcac ggccaggaat acaggcaata ccgcgctcgc ccgccgccca gtcacctgcc
     1561 ggtaaattgt gcagcgtatg ttcgagttta ttactcgcca gtacctgttt taattcttca
     1621 atgtcgtagt cataaggaaa cataaattca acgccgcgaa aaccacactg cgcagctttc
     1681 tcaaaacggg cgagaaaatc atattcacca aataacatcg ataaattagc agagaaacgt
     1741 aacatcattt atctccctta ttcatagtgc atgaagcagg tttcagtcgg tgcatccgct
     1801 gcgttatcgg cgatatcttc aaattccatg acgttatcca gttcgctgcc catcgaaata
     1861 ttggtcacac gctcgagaat aacttccacg actaccggta cccgatattg cgccattaag
     1921 gctttcgcct gttcaaaggc tggcgcaata tcttccggtt tgaagacccg aatagcttta
     1981 caacctaaac cttccgctac ttttacgtgg tcaacaccgt agccattcac ttcactggag
     2041 ttgatattct cgaaagcgag ttgcacgcag tagtccatgt caaaagcgcg ttgtgactga
     2101 cgaatcaggc cgagataggc gttattcacc agcacatgga tgtacggaat gttgaactgc
     2161 gcgccaacgg ctaactcttc aatcaggaac tggaagtcaa agtcgccaga aatcgccacc
     2221 acattgcgtt tcggatcagc ggcacaaacc cccagcgccg ccggaatcgt ccagcctaac
     2281 ggaccagcct gaccacagtt gatccagtgg cggtctttaa agacatgcag catttgtgcc
     2341 gcagcgattt gtgacagacc aatagtggtg acataacaaa catcgcgacc aaaggctttg
     2401 ttcatctctt catacacgcg ctgcggtttc accggcacgt tgtcgaagtg ggttttgcgc
     2461 agcaaagtgc gtttacgctg ctggcagtcg gcgacccatt ctttacgaca cggcagacga
     2521 cccgcttttt gcatctcctg cgccacttca accagcagtg tcagcgccgc tttagcatca
     2581 gagacaatgc cgagatccgg acacagcacg cgaccaattt gcgtcggctc aatatcaata
     2641 tgaacgattt tgcgcccttc ggtgtatttc tctaccgaac cggtatgacg gttagcaaaa
     2701 cggttaccga taccaaacac catgtcggac gccagcaacg ttgcgttacc gtaacgatgc
     2761 gcggtttgca gaccgaccat cccggccatc agttcatgat cgtccgggat acagccccag
     2821 cccatcagcg ttgggatcac cggaacgctg gtcagttcag caaactgttg taacagtgca
     2881 gctgcgtcag cattgattac cccgcccccg gcaacaatca ccggacgttc ggcctggatt
     2941 aacatttcta cagctttttc gatctgcata cggctggcag caggtttgta gaccggcagc
     3001 ggttcgtaca tgtcaggatc aaactcgatt tccgcaacct gaacgtcgaa cggtaaatcc
     3061 accagtaccg gacccggacg accagaacgc atcaggtgaa atgcctgttg cagcacgcga
     3121 ggcaccagcg ccgcttcacg aactgtaacc gccattttgc tgaccggttt agcaattgct
     3181 tcaatatcta cggcctgaaa atcttcttta tgcagacggg cgcgcggtgc ctggccggta
     3241 atgcacagaa taggaatgga atcagcagaa gcggaataga gcgcggtgat catgtccgtg
     3301 cccgcaggac cggaagtccc cagacatacg ccgatatccc ctgccgttgc gcgggtataa
     3361 ccttccgcca tgtgcgaaac accttccaca tgacgcgcca gaatgtgacg aataccgccg
     3421 tgcttacgca tcgctgagta gaacggatta attgcagctc ccggaacgcc gaaggcggtg
     3481 gtgatacctt ctttctccag cacatacatt gccgcgtcaa cggctctcat ttttgccatt
     3541 ttattcctac ctctattaat tggaaaaatt ttccaacttt caaattttgt caaaaaaacc
     3601 cgctccgcag agcgggatgc gacagacact atggatgtgc tttcagtccc aacgccgtgc
     3661 tgatatcgcg ggcggtgtct ctgaccagct caccctgact gacaaaacga tcttctgtca
     3721 gtcttgatga aggcccggag atggagatag cggcaacaac gctaccgaca tcatcgtaaa
     3781 ttgctgaagc tatgcaattc agacctacaa catgctcttc tttatctacg gtatagccca
     3841 gttcacgcgc ttgttccagg tccttcagca aggtgggcat atccacaagc gtagttggcg
     3901 taaactgctg caaaccggtt tgcagaatga tgctcatcaa ctcctcttcc gccagcggat
     3961 aaagcagcgc tttgcccgca ccggaagcat gcagcggcag acgactgccc agtggcgcac
     4021 acatcctgac catcgattta cactctaact gaccaattaa taccgcttca ttgccgttac
     4081 ggatcgcgac attgaccgtt tcgccggaaa gtaacatcag gcggcgcata aacggcccgg
     4141 cgacggagag gacatcgcgg ttatggatgt acgccgcacc gacgttaaag acacctaatc
     4201 ctatatgcca ccagcctaat tgactgtcct gatagacaaa atccgctgct tgtaaaacct
     4261 tcagcaagcg aaaggtcgtg gagagcggca aatccagatt gagagaaata tcgctaaccg
     4321 acgaacttcc cccacttttt tccagatatt gcagaatcgc aattccccgc tctaacgcct
     4381 gtgcgccctt ctgtgccaca ggctccgcct gtcctggcct gccgcgccgt ctaacttccg
     4441 tcatacctac gttaactccg acctacaagt gagcataaac aatgaacaat tagtttatct
     4501 tatgcaagtc ggttgcaggc gtcacgcaaa actgagttcc tgttcaggaa tactttcaac
     4561 atcacagttg tcactgccgc cgcgatcgat ggtcagaaaa tcggtgacgc gctgccaggc
     4621 gaaaagtggg tgatgccaga cgttacgatg gtaattcacc ccctgttcac cgttggtgat
     4681 aaacgcccgc agcgttgaca ggtctggttt gtcgtcgcct aacgccacga ccaccacaaa
     4741 cacttcacct ttcattggga taaaggcctg agtgcccagc ggatgacgtt cgagttcgtg
     4801 aatggtcagc ggcagattcg ccggttgcgc gcggttaatg ctgataagcg tacggtcttg
     4861 ctcaagaatc tcaaccagcg ccaaatcgtg gtaacgctcc accaggccat tgttaatatg
     4921 gaaaaaatcc cgttgctgcg tttcgattac gtcgccataa gcactaaagg cttcctgact
     4981 taacggtaat acctgaagtt tcatcttttt gtccttgtaa cataattccg ttcaaccacc
     5041 gccgaccggt cagcttggtt tcaataacag gcaatctaaa accttaagag aacaacatca
     5101 atacaaaagt tggaatgttt ttccaaaact agatttagtc cacaaattga acagggctat
     5161 catgtaaaaa tactgataaa tcagaggata agaaaatcac gcaacttgcg gatttaaaag
     5221 gggatacctg aagatgttcg atccagaaac cttgcggact ttcattgcgg ttgctgaaac
     5281 aggaagtttt tcaaaagcgg cagaacgatt atgtaaaacc acggcgacga tcagttatcg
     5341 cattaaactt ctggaagaga ataccggagt agcgctgttt ttccgtacga ctcgtagcgt
     5401 aacgttgaca gcggctggcg agcatctact ttcccaggcc agagactggc tgagctggct
     5461 ggaaagtatg cccagcgagc tgcaacaggt gaatgatggc gtggaacgcc aggtgaatat
     5521 tgtcatcaac aacctgctct ataactccca ggccgtcgcc cagttgctgg cgtggctgaa
     5581 tgagcgttac ccctttaccc agtttcacat ctcccgacaa atctatatgg gcgtctggga
     5641 ctcgctattg tacgaaggtt tttcgctggc tatcggggtc acgggaactg aggcgctggc
     5701 aaataccttt agtcttgatc ccttaggatc ggtgcaatgg cgatttgtca tggcggcgga
     5761 tcatccgctg gcgaacgttg aagagccgct aacagaagcg cagttgcgtc gctttccggc
     5821 ggtcaatatt gaagacagcg cccgcacctt aaccaaacgc gtcgcctggc gattgccggg
     5881 gcaaaaagag attattgttc ccgatatgga aacgaaaatc gccgcccatc tggcgggtgt
     5941 tggcattggt tttttgccaa aatcactttg ccagtcaatg atcgataatc aacaactggt
     6001 cagccgggta atcccaacga tgcgccctcc ttcgccattg agtctggcat ggcgcaaatt
     6061 tggcagcggc aaagcggtag aagatattgt gaccttgttt acccaacgca ggccggaaat
     6121 cagcggattt ttagaaattt tcggcaaccc acgcagttaa aaatcaagtt tccttgcgcg
     6181 ttggcgcgta aaatagcgcg caagctgatt tgaggttcac ttttaactat gcaagagaga
     6241 cacacggaac aggactatcg tgccctgctg attgctgata cgcccattat tgatgttcgc
     6301 gcccctatcg agtttgagca aggcgcaatg cccgccgcta tcaatctgcc gttaatgaat
     6361 aacgatgaac gcgccgccgt tggcacctgc tataaacagc aaggctcaga cgcagcgctg
     6421 gcgctggggc ataaactggt ggcgggtgaa attcgtcagc agcgtatgta cgcctggcgg
     6481 gcagcgtgcc tgcaaaatcc gcaaggtatt ctctgctgcg cccgtggcgg tcaacgttca
     6541 catattgtgc aacgctggct gcatgaagcg gggattgatt atccgctggt ggaaggcggt
     6601 tataaggcac tgcgccagac cgcgattcag gcgaccattg aactggcaca aaaaccgata
     6661 gtgctgattg gcggttgtac cggcagcggt aaaacgctat tagtgcaaca acagccaaac
     6721 ggtgttgatc tggaagggct ggcgcgtcat cgcggttcgg cgtttggtcg cacgttacaa
     6781 ccacaactta gccaggcgag ttttgaaaac ctgctggctg ccgaaatgct aaaaaccgac
     6841 gcccgtcagg atttgcgcct gtgggtgctg gaagatgaaa gccggatgat cggttcgaat
     6901 cacctgccgg aatgtctgcg cgagcgaatg actcaggcgg cgattgcggt ggtagaagat
     6961 ccgtttgaga tccgtcttga acgcctgaat gaagagtatt tcttgcgtat gcatcatgat
     7021 tttacccacg cttatggcga cgaacagggc tggcaggaat attgcgaata cctgcatcac
     7081 ggactttcgg cgattaagcg tcggctgggg ttacagcgct ataacgaact cgctgcccag
     7141 ctggatacgg cgctgacaac gcaactcacc accggcagca ccgacggtca tctggcctgg
     7201 ctggtgccgt tacttaaaga atattacgac ccaatgtatc gctatcagct tgagaaaaaa
     7261 gcggaaaaag ttgtctttcg cggtgagtgg gcggaagtgg cggtatgggt taaggcgcag
     7321 tgatgggcgg tattctgaaa cggcgattgt cgtgagaaca aaatggttgc accagaatac
     7381 agatataaaa accgacatga cagtcggttt ttttattttt tccgcccaag acttggtggt
     7441 tccgggcaga aattaaacag tcctcatcta gcaagtgaat tataagtcag gatgcctaca
     7501 tattcaaaca gctatgttgg cgatacaatc ccgacgctag tagtgatact gcctcaaggg
     7561 attaccgcag tgcggaaaag tacttttaag tcctggtcag gatacaggtg gtgctgattc
     7621 tggtgctcta atttggagag aggcagtcct catctagcac atgaagaaaa tcatagttac
     7681 aggcaacgta agggtaagct gtgattgtct ttctccctta agggagcaac ggatgaaaac
     7741 ttttctcgat aaagttctca tcgttcttaa ggcgctaatc gcgctgctgg aactgatccg
     7801 tcagtttatt gaataactga ggaaacgtag ctaaggccga aggtttacct ccttcggcct
     7861 ttaaaaaaac cgacatgaca gttggtttca tatttattgc caaaatgcac actgttcatg
     7921 ccagatgcgg cgtaaacgcc ttatccggcc tacacaatct tgcaaaatca atatattgca
     7981 cgctcttcgt aggcctgata agcgtagcgc atcaggcaat ctaacacctg tcatcacccc
     8041 acaaactgcc tgaacagcgc cttaccctta accagtcgcg cacccagcca gccgccgaaa
     8101 agcgacagca gcagcgcacc gctgcacggc agaacaatcc acaatcgcca gtctggctcc
     8161 cacgggaaat caaacacttt cgcctgcaac actgccagtg ccgtttccgc accaattgcg
     8221 gccaccaggc cggaaacaaa cccaagcatg gcgaactcac accacaatgt cgtgcgcagc
     8281 agttttttcc ccgcaccgag tgtgcgccat accaccagct cctgatgacg ctggcgcatt
     8341 cccacctgca cctgtgccag caacagcaac ataccgcagg cggtgaccag caccaccatc
     8401 acctccagcg cccgacttac ctgctccagc acctgaccga cctgttttaa aatcgcgcca
     8461 atgtctaaca ggctaatggt cgggaactgg cggttgagtt gcgtcaacat gccgttgcca
     8521 ttctcccagc ggaaactggt aagccagctc tgcggttgcc cgtctaatgc cccttcaggg
     8581 aaaataaaat agaaattagg ccgcagactt tcccagtcca ctttgcgcag gctggtcact
     8641 ttagcgcgga actcctgggt atcgcccata aaagtcacgg tatcaccgag ggcaacgttt
     8701 aagcgtttcg ccagcccctc ttccatcgac acttcatcgg cttttggcgg ccagttaccg
     8761 gcgacaatcg gattatgatc gggccgcgta ttttgccagg taagattgag ttcgcgatta
     8821 agcgcctcat cttcattacc ttctgtcggc ttatcgttaa tcgccgtcag ccgcgcccgc
     8881 actaccggat aaaatgattc cgggactata tgatgttccg cgaggaacgc ttttagcggt
     8941 gctacctgtt ctgtggcgat gttaattaaa aagtagttcg ggctttctgg cggtagctgc
     9001 tgctgccagc ggtcgagcag atcgccacgc agcaccagca gcagtgccag cagcataaag
     9061 gagagcgaaa atgccgaaag ctggcttaac gttgaccacg gctgacgtaa caggcggcta
     9121 accgccaggc gcagaggcag cgatttcagc gtcatgcggc gaagtacatt cagcagcatc
     9181 cagcccagca caccgcacag caaagccagt actaccgcgc ccgccagcac cgcccaaagc
     9241 agcatgctgc cacccattaa tccggcgagc agcagcacaa ccaccacact gacaatcggc
     9301 agataaaact tcagcggcca gacgttcgcg accacatcat tacgtaatac gcgtaaaggc
     9361 tgcgttgcca gcaacaagcg gtatggtcgt agccccacca gcagcgagat gaccgtcatg
     9421 gtgccaagcg cccacagcca cggccagagg ctggctggcg gtagtgcagc aggcagaacg
     9481 ggcttgagca gcaccatcaa cacgttttcg aacaacaggc ctatggcccc accggtaacg
     9541 gctgaaagcg tcagcaccat caaccactga ccgacgatta gcttacgcag ttgcgctcgc
     9601 cctgccccca gcgttttgag gatcgccacc agatcgtagc gactgcgaca gtaatgattc
     9661 atcgctaccg ccaccgctgc cactgccagc agcaaggtca gaagcgccga aagcagcagg
     9721 aactgttgcg agcgttccat cgatcgcccc agcgcgcctt cgtcctgttc cagaccgtac
     9781 cagcgttgtt cgggtttaag ctgaggtaac aaccatttct catagccgtc gagctggttc
     9841 tcgttgccgc cgaatttata acgccaggtg acccgactcc ccggctgcac ggctccggtt
     9901 ttatcgacat ccgccagatt catcatcaga cgcggagcca tctggaaggg gttaaaaccg
     9961 gaatccggtt cctgaatcac ttctccggca atccgcaagg tggcatcgcc cacgtcaatg
    10021 gtgtcgcccg ttttcaggtt aagcagtgcc atcaggcgtg gggccagcaa tacgctgccc
    10081 gcctgcggtt tcaggccagg gggattagtt tgcagatcgc catacatcgg gtagatatca
    10141 tccaccgctt tgacgttcgc cagctgcggt gtgtcgcctg caaaggtcat tgtggcgaaa
    10201 gtcagctgct tgccgacttt caggccgcgc ttttgcgctt cctccagcca cgcttgcggc
    10261 acttcgcgtg aactgcgcaa cgcccgatcg cccgccataa actcacggct ttgctggctt
    10321 aagcccttct ccatgcgatc gctgatattg cccagcgcca gcacgcaggc caccgccagg
    10381 cttagcgcca gccagacaat taatagcgac ggcgaacgcc attcgcgcca gaaccaacgt
    10441 gcaatcatgc ttcctcctgc aactgcccgt tcaccagccg taagcagcgg tcgcagcgtg
    10501 ccgccagttg caggtcgtgg gtcaccataa tcaacgtggt gccatgttca cggttgaggg
    10561 aaaacagcag gtcggcaatt ttatcgcccg tctggcggtc aaggttgccg gtgggttcgt
    10621 cggcaaacag cacatcaggt cgaccattaa aggctcgtgc cagcgccact cgttgctgtt
    10681 caccgccgga aagctgcgcc ggaagatgat ccagacgttt acccagccct aactgttcga
    10741 gcaacgcttt cgccccgtta cgactttccg cgctactctc accgcgcagc agagccggaa
    10801 gctcgacgtt ttccagcgcg ttaagggtag gaattaacat aaatgactga aaaacaaagc
    10861 cgacgtgctt cgcgcgcaac tttgcccgcg cttcttcgtc catattatgt agcggttgtc
    10921 ccaccagact cacttcgcca ctgctgccgt catcaagccc ggcgaggatc gccagcaagg
    10981 ttgacttacc cgatcccgac tcgcccacca gtgcgatggt ctcgccacgt ttgacaacca
    11041 gctcaactcc ggtgaggatg gagagttcat gctccccctg accgacggac ttcttaagat
    11101 gatgaacttc aacaatgttt tccgctggca tttgcccttc ctgttcctgg tcctgttaac
    11161 cttccgtgcc gccgcagcgg acacgttatt gattctgggt gatagcctga gcgccgggta
    11221 tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc agagtaaaac
    11281 gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caagggctgg cgcgccttcc
    11341 ggctctgctc aaacagcatc agccgcgttg ggtgctggtt gaactgggcg gcaatgatgg
    11401 tttgcgtggt tttcagccac agcaaaccga gcaaacgctg cgccagattt tgcaggatgt
    11461 caaagccgcc aacgctgaac cattgttaat gcaaatacgt ctgcctgcaa actatggtcg
    11521 ccgttataat gaagccttta gcgccattta ccccaaactc gccaaagagt ttgatgttcc
    11581 gctgctgccc ttttttatgg aagaggtcta cctcaagcca caatggatgc aggatgacgg
    11641 tattcatccc aaccgcgacg cccagccgtt tattgccgac tggatggcga agcagttgca
    11701 gcctttagta aatcatgact cataaagcaa cggagatcct gacaggtaaa gttatgcaaa
    11761 aatcggtctt aattaccgga tgttccagtg gaattggcct ggaaagcgcg ctcgaattaa
    11821 aacgccaggg ttttcatgtg ctggcaggtt gccgaaaacc gaatgatgtt gagcgtatga
    11881 acaacatggg atttaccggc gtgttgatcg atctggattc accagaaagt gttaatcgcg
    11941 cagcagacga ggtgatcgcc ctgaccgata attgtctgta tgggatcttt aacaatgccg
    12001 gattcggcat gtatggcccc ctttccacca tcagccgtgc gcagatggaa cagcagtttt
    12061 ccgccaactt tttcggcgca caccagctca ccatgcgcct gttacccgcg atgttaccgc
    12121 acggtgaagg gcgtattgtg atgacatcat cggtgatggg attaatctcc acgccgggtc
    12181 gtggcgctta cgcggccagt aaatatgcgc tggaggcgtg gtcagacgcg ctgcgcatgg
    12241 agctacgcca cagcggaatt aaagtcagcc tgatcgaacc cggtcccatt cgtactcgct
    12301 tcaccgacaa cgtcaaccag acgcaaagtg ataaaccagt cgaaaatccc ggcatcgccg
    12361 cccgctttac gttgggaccg gaagcggtgg tcgacaaagt gcgccatgct tttattagcg
    12421 agaagccgaa gatgcgctat ccggtaacgc tggtgacctg ggcagtaatg gtgctcgagc
    12481 gcctgctgcc tgggcgcgtg atggacaaaa tattgcaggg gtgagttgaa gcgcgcgctt
    12541 aagcccccat gtcaaagaaa atatcgacaa cagagagcga ctccatgtcc gtagaaaata
    12601 ttgtcaacat taacgaatct aacctgcaac aggttcttga acagtcgatg accactccgg
    12661 tgctgttcta tttttggtct gaacgtagcc agcactgttt gcagttaacc ccgatcctgg
    12721 aaagcctcgc ggcgcagtac aacgggcaat ttattctggc gaagctggac tgcgacgcgg
    12781 agcagatgat tgccgcccag tttggtctgc gcgcgattcc gaccgtgtat ctgttccaga
    12841 acgggcaacc ggtagatggc ttccaggggc cgcaaccgaa agaggcgatc cgcgccctgc
    12901 tggataaagt gctgccgcgc gaagaagagc tgaaagcgca gcaggcgatg caactgatgc
    12961 aggaaggcaa ttacaccgat gccctgccat tgctgaaaga cgcctggcag ttgtcgaatc
    13021 agaacgggga gatcggcctg ctgctggcag aaacgctgat tgcgctgaac cgttctgaag
    13081 atgcggaagc ggtgctgaaa accattccgt tgcaggatca ggacacccgc taccaggggc
    13141 tggtagcgca aatcgaactg ctgaagcagg cggctgataa gccggaaatt caacagttgc
    13201 aacagcaggt ggcggagaat ccagaagatg ccgcactggc gacgcaactg gcgctgcaac
    13261 tgcatcaggt tgggcgcaat gaagaggcgc tggagttgct gttcgggcat ctgcgtaaag
    13321 atctcaccgc cgcagacggt cagacgcgta aaacgttcca ggagatcctc gctgcgctgg
    13381 gtacgggtga tgcactggcg tcgaagtatc gccgccagct gtatgcattg ttgtattgat
    13441 attccgttgt tcggcacatg aaccacaacg ccggatgcga tgctgcgcat ccggcatcgc
    13501 atcatttctt cttcaattga gtcaccacca actggtggcg cgaattataa aacttacgat
    13561 aggttaaata gcaggcaata atggtcgaca agctggcggt tgagagcagc ataaaggtca
    13621 ccataatctg atatttaatc gccttcaccg gatcaatccc ggcaaatatc agcccggaca
    13681 tcattcctgg taaactcact aagccaaccg tttttgctga atcgaccgtc ggaattaaag
    13741 ccgcgcgaat actgtcgcga atcaatatcg ctgaagcctg cttaggcgtc gcgccaagac
    13801 tcagtttctc ctgaatctgt tgctgttcgc taatgacccg ttgccctaaa ttgttgtaac
    13861 acaaccccac cgctaccatg gcgttaccgg caatcatccc ggcgacaggg atcacctgca
    13921 tcgggataaa ttcaatcgac cctgagagaa tcagcaccgc cagggtaatt cccgccccga
    13981 ccgtaatggc aataaacgat gagataaaag ctttagcaat atatttactg cgtttttgcg
    14041 cgttccacgc cgcattaaag cagataaata acaccatcaa taatgtcagg ctggcatcat
    14101 ccacgctgaa aatatacttc agcacatagc cgacaataat cagctgaatt atcgctcgcc
    14161 cgacgctcca gagaatatct ttctccagcg ccagtttttc tttatggcta attaagattg
    14221 ccaccaccac cagcattaat gccagtgcta atgattcgtt agtaatatta tgcgagttca
    14281 tagcgtgctt cctgcatttc tccggcatgc ggttgcagtg taatcacttt atccgcatga
    14341 ttaatttcgt ctttatcgtg tgtcacccac agcacggcaa tattttgctc gcgcacataa
    14401 cgatggatca tctcattgac gttatgttta ttactttcat ccagcgcact ggttatttca
    14461 tccagcaata aaaccttcgg cataaattgc aggttacgaa tcaatgagat gcgttgtttt
    14521 tcaccaccag atagctcggc gatattcttc gtcaaaatgc tgtccggcaa agcgaagcgt
    14581 tcgagaaaat cgagaaaaat ggccgggtca ggctgctggt cacggatctg ccagggaaag
    14641 atcagattat cgtataccgt atcgccaaac agcgtcggcg tctgggcgca gtaagagact
    14701 tgctggcggt agatttctgg ttttagtgtg ctgacatcct caccttcaaa cagtaacgtt
    14761 ccgctggttg ggctgatcaa tgaagcaact atttttagca gcgtactttt gccacaacca
    14821 gaaggaccgg taattaactt aaattcgcca gcacgcagcg aaaaattgat gttattaaga
    14881 atcttcgtat cacccgccag atatcctacg ttttgtagct gaagcaaagg actgttttcc
    14941 tgcatcgctg ttcccttttt ctgattttta ctaaaaacag tttatccttc gcaggaataa
    15001 gggggaactc tctttcagta atcaggtata atttgtttaa tcaagcagca attttgctga
    15061 ttatggacgg tacaacagga ggtttttccg atgcttatct ttatcccgat tctcattttt
    15121 gtcgcgctgg tcattgtcgg cgcgggcgtc aaaatcgtgc cgcagggcta tcaatggacg
    15181 gtagaacgtt ttggtcgcta taccaaaacg ttacagccgg ggctcagtct ggtggtgccg
    15241 tttatggatc gcattggtcg caagatcaat atgatggagc aagtgctcga tatcccttcc
    15301 caggaagtta tctcgaaaga taacgccaac gttaccatcg acgcagtctg ttttattcag
    15361 gtgattgacg cgccacgcgc tgcctatgaa gtcagcaatc tggagctggc gatcatcaac
    15421 ctgaccatga ctaacatccg taccgtgttg ggttcaatgg aacttgacga aatgctctct
    15481 cagcgcgaca gcatcaactc acgcctgctg cgtattgtcg atgaagccac caacccgtgg
    15541 gggattaaag tcacccgtat tgaaattcgc gacgtgcgcc caccggcaga gcttatctct
    15601 tcaatgaacg cgcagatgaa agcggaacgt accaaacgcg cttacattct tgaagcggaa
    15661 gggatccgtc aggcggaaat cctcaaagcc gaaggtgaaa aacagtcgca aatcctgaaa
    15721 gcggaaggcg aacgtcagtc ggcgttttta caggctgaag cgcgtgaacg ttccgccgaa
    15781 gcagaagccc gcgccaccaa aatggtgtct gaggctatcg cctccggtga tattcaggcg
    15841 gtgaactact tcgtagcgca aaaatacacc gaagcgttac agcagatcgg ttcctccagt
    15901 aacagcaaag tagtgatgat gccattagag gccagcagcc tgatggggtc gattgccggg
    15961 attgccgagc tggtgaaaga cagcgccaac aaacggactc agccatgatg gagttaatgg
    16021 tcgttcatcc acatattttc tggctcagtc tcggcgggtt gctgctggca gccgagatgc
    16081 tgggcggaaa tggttatttg ttgtggagcg gcgtggcagc ggtgattacc ggcctggtgg
    16141 tctggctggt gccgctgggt tgggagtggc aaggggtaat gtttgccatc ctgacgctgc
    16201 tcgccgcctg gctgtggtgg aaatggttgt cgcggcgggt gcgcgaacaa aagcacagcg
    16261 atagtcattt aaaccagcgc gggcagcagc tgattggccg acgttttgtg ctggaatctc
    16321 cgctggtcaa cgggcgcggt catatgcgcg tcggtgacag ttcatggcct gtcagcgcca
    16381 gcgaggatct cggtgcaggt acgcatgttg aagtcattgc gatagaaggg ataaccctac
    16441 atatccgagc cgtctcgtct taatcaccct gcccgatgat gacagcagcc ggagagattt
    16501 tcgataatcg ggcagtcggc gctgtcatcg ccagggcagg cattcgccag tgccagcagc
    16561 tggtcgcgca tggattgcag ctcctcaatg tgtcgttcga tctccgccac cttctccagc
    16621 gtgcgccgtt tgacgtcggc gctgtgccgc tgcgggtcgt taaacagatt caccagctcg
    16681 ccgctctctt ccaggttaaa gcccacctgc cgtgcctggc gcagtaaggt cagttcgttg
    16741 agatgctgct gcgtgtaggt gcgataaccg ttttcgctgc gcatcggcgg cgtcaccagc
    16801 cccttctctt catagaagcg aatggctttg ctggtcaggc cggtaatttt tgctacatcg
    16861 ctgatgttca tcgttcgcgc aacgcctcct gtgctttgtt aaagggtttc aacagatatt
    16921 caagcacgtt tttttgccag tgcgaatatc gaccgccacc acaataatac aggaagtact
    16981 gcgcacagag aggtgttata cggttttatt acgcttcatg accattgcca caataaggct
    17041 gagtaagatt gcaccaataa tccatatcaa cgcacggctg ccctgggacc acaggctgta
    17101 gataaagcca acgatcacca acaacatcac caacgtacca acgataagaa tcggcaacga
    17161 ggcgtgaata tcatgacgca ggcggatcgc caccacaaac accgccagat agcagatcaa
    17221 aaaggtggcg ctggcaacgc tggcgagtga gtctaaattt agcgccgccg tcatcagcat
    17281 tatcaacacc acgacgatga tgttgcccca ggtactctgc cgccacaggg atttattcat
    17341 tagcttcggc agttcgcgtt cgctgcccat gttgtccatg atgttaaaca cggcgaacag
    17401 gttcgcgtta atgcctgaag ccgtcgccag taaagcgccg atgacgacga tcacataacc
    17461 cacatgcccg agcagcggag aagcagcctg cgctacggcg gtatcagcat atttttctaa
    17521 ctctaatgcc gatacatcgc taagcaaaac cagcgccagc gagatataaa gcaacgtggt
    17581 aacgccaatc gccaccagaa acgcccgtgg cataatgacc tgcggatctt tcactttatc
    17641 cgccgcgttc gccatcatgc caaagcccgc ataggcaagg aaggttatcc caatacagga
    17701 gaagaacgca ccggagctgg ggggcgcaga gacggaaata tgcgccggtt gcagcgacca
    17761 gacaccggca ataatcagca ataacaggat catcatttta atgccgacga ggatcacttc
    17821 cagccgccct accgcatggt tgcttaagga gttgaaaagc gtcatcaccg caatgatccc
    17881 caacgcgtag agcaaaataa ggtgctcctc ctggctgcct tcatgcaaaa actgcacggc
    17941 ataagcgcca aaagcacggg caaccatggc gatgctcacc gccagcgtca acaggtatag
    18001 caacgagagc gctagcgaaa agacgccgtt gcctaatccg cgacgaaaaa agtcgataat
    18061 accgccattg cagggatagc tcgcccccag acgcgcatag gcataaccgg aaaacatcgc
    18121 cacaataccg ccaaaagcaa aggcgcccca ggtcgaggct tccattagca acgcagcctg
    18181 ccccagcagc gcgaagatcc ccgcccccac cattgcccca ataccgatgg aaacgacgtt
    18241 ccatagaccg aggggtttgt taccgttatt accttccgtg ttcatcatga tcagccctta
    18301 aacacgttat agccgagttg cttagcgacc gatgccacca ttttttgacc gcgaacacta
    18361 ttgccttctt cgtccagcgg tggtgagaac gcggcaattc ccatcacgcc agggacgacc
    18421 gccagaatac cgccacctac accgcttttg cccggtaaac caacgcgata cgcccagtca
    18481 ccggagcgac catacagccc ttccatcatc atttcggcca gaatgtacgg cacgttgtcg
    18541 gcctgaagaa cgcgtttatg cgtcaacgga ttcacaccac ccgccgccag cgtcgcgcca
    18601 agcgttgcca gttcaacagt attgatgagc gtagagcact gacgggtgta cacgtcacag
    18661 gcttccattg catcacaata gagatatccg gcggagtaca gcagccaggc aatggcccgg
    18721 ttatggaagt tggttgtttg ttccgactgg ttgacttcgt cagagagcgc tacctgctca
    18781 ccagccagtt gctgttggat atgtaaaatt cgctgccagc gttgttcggc gttttcagcg
    18841 ttaatcaggc tggtggtggc aatagcgcca gcatttacca gtggcgaaag cggtttgccg
    18901 ccatgcaact ctaaggcgat aactgagtta aagggcaatc cggtcgggtc agcgccaatt
    18961 ttgtcctgta ccgcctgcgg gccgacatct tctaacgcaa gggctaacgt acagacttta
    19021 gagatggatt ccagtgcaaa gcggtaatca ctgtcacccg cactatagac gttgccatcg
    19081 caggtcacga tagccactgc cgccagttga cctggtacat tcgccagaaa gggaatgtaa
    19141 tcggcatttt gtccgccgtt aagtgagtga aattgggtgt aagcctgatc cactgcctgc
    19201 tgtaatttgt ttgcatctaa catcttttgt taactccttt ttatagatgc gggaggtaat
    19261 tcctcacccc ggtgccgatt ttcaggcatc ctgatttaac ttagcacccg caacttaact
    19321 acaggaaaac aaagaaataa atgtctaatc ctgatgcaaa tcgagccgat tttttaatct
    19381 ttacggactt ttacccgcct ggtttattaa tttcttgacc ttccccttgc tggaaggttt
    19441 aacctttatc acagccagtc aaaactgtct taaaggagtg ttttatgtca caaactatcg
    19501 acctgaacct ggacggcctg tcctgcggtc actgcgttaa acgcgtgaaa gaaagtcttg
    19561 aacagcgccc ggatgtggaa caagcggatg tgtctatcac tgaagcgcac gttaccggga
    19621 ctgccagtgc agaacagctg atcgaaacca tcaaacaagc gggttatgac gcatctgtaa
    19681 gccacccaaa ggctaaaccg ctggcggagt catcaatccc gtcggaagca ctgacagcgg
    19741 tttctgaggc gcttccggca gcgaccgccg atgacgatga cagccagcag ttgctgctga
    19801 gcggcatgag ctgcgccagc tgtgtcaccc gcgtacaaaa tgcgctgcaa agcgtaccgg
    19861 gcgtcactca ggcacgggta aacctagcgg agcgtactgc gctggtgatg ggcagtgcct
    19921 ccccacaaga tttagtgcag gcggtggaaa aagcgggcta cggcgcggaa gcgattgaag
    19981 atgacgcgaa acgccgcgaa cgccagcaag aaaccgccgt cgctacgatg aagcgcttcc
    20041 gctggcaggc aattgtcgca ctggcggtgg gtatcccggt gatggtctgg gggatgatcg
    20101 gcgataacat gatggtcacc gccgacaacc gcagcctgtg gttggttatc ggcctgataa
    20161 ccctggcggt gatggttttc gccggcggcc atttttaccg cagtgcatgg aaaagcctgc
    20221 tgaacggtgc agcgacgatg gatacgctgg tggcgctggg aacgggcgtg gcgtggctct
    20281 attcgatgag cgtcaacctg tggccgcagt ggttcccgat ggaagcgcga catctttact
    20341 atgaagccag tgcgatgatt atcggtctga tcaatctcgg ccatatgctg gaagcacgcg
    20401 cacgtcagcg ttcttctaag gcactggaga aattactcga tttaacccca ccgacggcgc
    20461 gcctggttac tgacgaaggt gaaaaaagcg tgcctctggc agaagtgcag ccaggtatgt
    20521 tgctgcgcct gacgaccggc gatcgcgtgc cggtagatgg cgagattacc cggggcgaag
    20581 catggctgga tgaagcgatg ctgacgggcg aaccaatccc gcagcaaaaa ggcgaaggcg
    20641 atagcgtcca tgccgggaca gtggtacagg acggcagtgt gctgtttcgt gccagtgcgg
    20701 ttggcagcca taccacgcta tcaaggatca ttcgcatggt gcgccaggcc cagagcagca
    20761 agccagaaat cggtcagctg gcggataaaa tctcagccgt atttgtgccg gtagtagtgg
    20821 ttattgcgct tgtcagtgcg gcaatctggt atttctttgg tccggcaccg cagattgtct
    20881 ataccctggt gattgccact acggtgctga ttatcgcctg cccgtgtgcg ctgggactgg
    20941 cgacgccgat gtcgattatt tccggcgtcg ggcgggctgc tgagtttggc gtgctggtgc
    21001 gggacgccga cgcgctgcaa cgcgccagta cactcgatac cgtcgtgttc gataaaaccg
    21061 ggacgctgac cgaagggaag ccgcaggttg tcgcagtgaa aacatttgct gatgttgacg
    21121 aagcgcaggc gctgcgtctg gcggcggcgc tggagcaagg ttccagccat ccattggcac
    21181 gagcgatcct tgataaagca ggcgatatgc agctaccgca ggtcaacggt ttccgcacat
    21241 tgcgcgggct gggcgtgagc ggtgaagcag aaggtcatgc gttattgctg ggtaatcagg
    21301 cgttgttaaa tgatcagcaa gtcgatacta aagcgattga agccgatatt tcagctcagg
    21361 catcgcaagg agcaacgcct gtgctgctgg cgattgacgg gaaagcagta gccctgctgg
    21421 ctgtacgcga tccgttgcgt agtgatagcg tggcggcgct gcaacgcctg cataaagcgg
    21481 gatatcgtct ggtgatgttg accggggata acccaaccac cgccaatgcg atcgccaaag
    21541 aagctggaat tgatgaagtg attgccggag tgctgccgga cggtaaagcc gaagcgatca
    21601 aacgtctgca aagtgaagga cgtcaggtgg caatggtggg cgacggcatt aacgacgcgc
    21661 cagcgctggc ccaggcggat gtcggcattg cgatgggtgg cggcagtgat gttgccattg
    21721 aaaccgcggc gattaccctg atgcgccata gcctgatggg cgttgcggat gcgctcgcga
    21781 tttcccgcgc aacgctgcgc aacatgaagc agaacctgct cggtgccttt atctacaaca
    21841 gcatcggtat tccggtcgcc gccggtattt tgtggccgtt tactggaaca ctgcttaacc
    21901 cggtagttgc cggagcggca atggcgctct cgtcgattac cgtagtgagt aacgccaacc
    21961 ggttgctgcg gtttaaaccg aaggaataag tacgtcattg cggatgcgct gctttagggc
    22021 ggcgcatttg gcatcgacgc atgactgccg gatgcggcgt aaacgcctta tccggcctac
    22081 ggttcaggca caggtttgta ggcctgataa gacgcgtaag cgtcgcatca ggcaatggtg
    22141 ctcggatgcc ggatgcggcg tgaacgcctt atccggccta cgagatgaag cgatgttttg
    22201 gccttacgcg accttttttg cacgctcttc acggcgtgcc aaatattcag ccactgtttc
    22261 aatccgtccc aattcttcct gggtgcgcat gttgttttca acttcccaaa gatcaactgc
    22321 cgcctggagg tttagcctaa aatcgactgt ggtatcaaaa actttcgcca gacggaatgc
    22381 catctcagtg gtgagtttac gattgttatt gatcagtgca ctgacgcgat tacgatgaac
    22441 atgcagcaac tctgctaact cattgatttt caaatcgagc ggttccagat attcatagag
    22501 aagaatatct ccgggtgtcg tcggttttct tgttgcctgt ttcatggttt ttaactgttg
    22561 cttccctgtg aatagcgatg caagaacata ctgaatcata caaacacatt aaacaagtcc
    22621 ccaactccat ttgcgccatc cctcaccgcg cgctagcatg atatttcaca aagggaggtc
    22681 gtatggatct gttgtaccgg gtaaaaacgc tttgggccgc gctgcgcggt aatcattaca
    22741 cctggcctgc catcgatatc accctccccg gcaatcgcca ttttcatctg attggcagta
    22801 ttcatatggg tagccacgat atggctcccc tgcccacccg tttgctcaaa aagctcaaaa
    22861 acgccgatgc gctgatcgtc gaggcggatg tttccaccag cgatacgcct tttgctaatt
    22921 tgcctgcctg cgaggcactg gaagagcgca ttagcgagga gcaactccaa aacctgcagc
    22981 acattagcca ggagatgggc atttctccct cactcttttc tacccaaccg ctgtggcaaa
    23041 tcgcgatggt tcttcaggcg acgcaggcac aaaaactggg gctgcgggca gaatacggta
    23101 tcgattacca gctattgcag gcggcgaagc aacaacataa acccgtgatt gaactggaag
    23161 gggctgaaaa ccagattgcc atgttgctcc agctccctga caaaggactg gcgctgctgg
    23221 acgatacgct gacccactgg cataccaacg cacggttgct gcaacaaatg atgagctggt
    23281 ggctgaatgc accgccgcaa aataatgata taacgctgcc caatacgttc agtcagtcgc
    23341 tgtacgatgt gctgatgcat cagcgaaatc tcgcctggcg ggataaatta cgcgccatgc
    23401 cgccggggcg atatgtggtc gcggtcggtg cactacacct gtatggagaa gggaatttgc
    23461 cgcaaatgtt gcgctaaaaa aatggccaat atcgctattg gcccgtcaaa gaggaatttc
    23521 atttttttat tattatgccg tcactttaag cgacggtgta actcgattgt cgctcaatct
    23581 caccatcctg ccaatactct taggcaagat gactcttttt tttggaccgc catcaggcgt
    23641 atgcttgctt cgtttttttg tttaaggcag gaaaattacc atgacacccg cagttaaatt
    23701 actcgaaaaa aacaagattt cgtttcaaat ccatacctac gagcacgatc cggctgaaac
    23761 caattttggc gatgaagtcg tcaaaaaatt aggtttgaat ccggatcagg tctacaaaac
    23821 gctgctggtg gcagtgaacg gtgatatgaa acaccttgcc gtggccgtta cgccggtcgc
    23881 aggtcaactg gatcttaaaa aagtagcaaa agcgctgggt gccaagaaag ttgagatggc
    23941 cgatccgatg gtcgcgcagc gttcgacggg atacctggtt ggggggatta gcccactggg
    24001 gcagaaaaaa cgtctgccaa cgattatcga cgcccccgca caagaatttg ccactattta
    24061 tgtttccggc ggcaagcgcg gactggatat cgaactggcg gcaggcgatc tggcaaagat
    24121 cctcgatgcc aaatttgctg atatcgcccg ccgcgattaa gcattgtgcc ggatgcaaac
    24181 atccggcact ttcggattac tgccagctca cctcaccttt cggttcataa acactcacat
    24241 ccagcggcga gcttttctgg atatacgctt tcagcacttc ggcatcaata aagccggtat
    24301 tcacatagcc cggattgtta tcaaggcgcg gatatccatc accgccggtg gcattgaagt
    24361 ttaatgtcgc catacggtaa gttttcgccg gatcgaccgg ttcgcctttg attttaaggt
    24421 cgttcagttt gccgtctttc gccacaaagc taacgttggc aaattgcggg taggcacctg
    24481 aatctggctt catctgcgcg acggcggtca ggtaatcaat cacctcttta ccggtcatgt
    24541 cggcatacac caccacattg ccgaatggct gcactttcag cacgttttta tagctgatat
    24601 cgcctgcttc gatagaatca cgaattccgc ctccgctcat caccgcaaag tcggcaccag
    24661 tgcgatccat ttgggctgcc agaatcaacc gccccatatt ggtctgtaca aaacgcactt
    24721 tgtcacgatc gccttccaga cgaccattgg tttcgcctat tttcacttcc agctgcgctt
    24781 tgcctttgtt ctggaacggt gataacagcg agatcatttg ctggttttca gcgatttcag
    24841 gagtgtaaag cacgcgctcg cttttcccgt cttcccaggt cactttcttc ttcaggttca
    24901 ccggaatcag ctggtagtta accattttca tttcgccatt acgaaactca aaatcagccc
    24961 gtcccacgta tttgccccac tcatgcgcct gcacaatcca gatgccgttt tgttgatctg
    25021 gtttgcatgg cgtacccggc acgtaatcga cctgtttttt gttttctgcc gccatgcaga
    25081 ccggatcttg cgagtgacca ccgacgatca tcgccagcga tccggcaggc agcgcgcgtg
    25141 ccatctccac atcgcccggt gcgttagagc cgtactcacc attatcgtaa tgccccatat
    25201 gggtcgccgc gataataatg tctggctttt ctgtctgttg cagctcctga atcaccagct
    25261 tcgcttcatc ggcgggctta cgaaattcga tatcagtgaa gtattccggg ttaccaattt
    25321 ttgctgtgtc atcggttgtc agcccaataa cggcaatttt cagatcctga cgcttaaaca
    25381 gcgcccacgg tttaaacagg cgctcgccag tacttttctg gtagatattc gcggaaagca
    25441 acgggaactt ggcccacttt tcctgctgac gtaatacggt gagcggatta tcaaattcat
    25501 gattaccgat cgccatcgcg tcatagccca ccagattcat accgcgaaaa tcaggttcgg
    25561 catcctgtaa gtcagactcg ggcacgccag tgttaatgtc gccaccggaa agtagcagca
    25621 cgctaccgcc ttcagccgca acctctttgc ggataccatc caccagcgtt ttttgcgccg
    25681 ccagaccata ttcgccatat tcattgcgcc aaaaatgccc atgatgatca ttggtatgca
    25741 gaactgtaat tttgtaggtt ttatcctgct catacgccag agcagtttca ctcgccagtg
    25801 taaatgtggt taacagcgct aacgccacgc cccgctgcaa taatttcata cttctctccc
    25861 tgacctgatt tcaacgtgtt gaaaaatagt atgacgcgac atcatagaca agtctgcgtc
    25921 cggtgaagat aaatctgcat ccagattaga aattctgtat tcggcgttga gacttccttc
    25981 agacgacgca tacaggtatg ttagtcgggt aataatcaca aataagatca ttctttacat
    26041 cgcaaccctg aaaaagtgac tttcctatgg caatgagtga acaaacccag cctgtggcgg
    26101 gcgcggctgc gtcaacgacc aaggcccgaa catcgtttgg tattttaggt gctatcagcc
    26161 tctcacatct gctgaacgac atgatccaat cgctgattct ggcgatttat ccgctgcttc
    26221 agtcagaatt ttctctgaca tttatgcaga ttggcatgat aaccctcacc ttccagctcg
    26281 cctcttcgct actgcaacca gtggtcggct actggaccga taaatatccg atgccgtggt
    26341 cgttgccaat tggcatgtgc tttaccttaa gtggtctggt gctgcttgcg ctggcgggca
    26401 gttttggcgc agttctgctg gcggcggcgc tggtcggtac cggttcatcg gtctttcatc
    26461 cggaatcttc tcgcgtagcc cgtatggctt ccggcgggcg gcatggccta gcgcaatcta
    26521 tctttcaggt cggcggcaac tttggcagtt ccctgggacc cttgctggcg gcggtgatta
    26581 tcgcgcctta tggcaaaggc aacgttgcct ggtttgtgct tgcggcactg ctggcgatcg
    26641 tggtgttggc gcaaattagc cgttggtact cggcacagca ccgaatgaat aaaggaaaac
    26701 ccaaagcgac gataatcaat ccactgccgc gcaacaaagt ggtactggca gtcagcattc
    26761 tgttaatcct cattttctcg aaatatttct atatggcgag catcagcagc tattacacct
    26821 tttatctgat gcaaaaattc ggattatcta tccagaatgc ccagcttcat ctgtttgcct
    26881 tcctgtttgc cgttgcggca ggtacggtga tcggcgggcc tgtaggggat aaaattggac
    26941 ggaaatatgt gatttggggc tctatcctcg gcgttgcgcc gtttacgctg attttaccct
    27001 acgcccgcct gcactggacg ggggttttaa cggtgattat tggatttatc ctcgcttcgg
    27061 cattctctgc cattctggtc tacgctcagg agctacttcc gggacgtatc ggtatggttt
    27121 ctggactctt tttcggtttt gctttcggca tgggaggtct gggagcggca gttctggggc
    27181 ttatcgccga tcacaccagc atcgagttag tctataaaat ctgtgctttc ctgccactat
    27241 tggggatgtt gaccatattc ctgcctgata accggcataa agactgattt cattgcagcc
    27301 aaaggcaaac tttggctgca tcgttttaca gtcgccataa gcctttcctc tgttaaaccg
    27361 ccttctgttt tccgatccgt gctttttttg cagctaattc catttttctg caaaattcaa
    27421 caattattca taaacatcat caatgaaatt gtcataaact attacctgta attttggttt
    27481 tcccggccaa aaatggacaa taaccaccag gaaaaggaga cggaatgcat cacgccaccc
    27541 cgcttatcac caccattgtt ggcggccttg tgctcgcctt tatcctcggc atgctggcca
    27601 ataaactacg tatttctcct ctggtgggat atctgttagc gggtgtgctg gcaggaccat
    27661 tcactccggg ctttgttgcc gataccaagc ttgcgccgga actggctgaa ctgggcgtta
    27721 ttttgctgat gttcggcgtc ggtttgcatt tttcgctcaa ggatttgatg gcggtaaagt
    27781 ccatcgccat tcccggcgcg atcgcccaga tagccgtggc gacgctgctg ggtatggcgc
    27841 tctccgccgt gctgggctgg tcgttaatga ccggtatcgt gttcggctta tgtctttcca
    27901 ccgccagtac cgtggtgtta ctgcgcgcac ttgaagaacg gcaattaata gacagtcagc
    27961 gtgggcaaat cgccatcggt tggttgattg tggaagacct ggtgatggtt ctgacgctgg
    28021 tgttgctgcc cgcagtggca ggaatgatgg aacagggcga tgtgggcttt gccactcttg
    28081 cagtcgatat ggggatcacc atcggcaagg tgatcgcatt tatcgccatt atgatgctgg
    28141 taggtcgccg tctggtgccg tggattatgg cacgcagcgc ggcaaccggt tctcgcgagc
    28201 tgtttaccct gtcagtgctg gcgctggcgt tagggattgc ctttggtgcg gtagagctgt
    28261 ttgatgtctc ctttgcactc ggtgcgttct ttgccgggat ggtactgaac gagtctgaat
    28321 tgagtcaccg tgccgcccac gatacgttgc cattgcgcga cgcgtttgcg gtgctgtttt
    28381 ttgtctcagt cgggatgttg tttgatccgt taattctgat tcagcaaccg ctggcagtgc
    28441 tggcgacgct ggcaattatt ctgtttggta aatcggtcgc ggccctgttc cttgtccgtc
    28501 tgtttggtca ctcccaacgt acggcattaa ccatcgccgc cagcctggcg cagattggtg
    28561 agttcgcgtt tatcctggcg ggactgggaa tggcattgaa tttactgccg caggccggac
    28621 aaaacctggt actggcaggg gcgatcctgt cgattatgct caacccggta ctgttcgcac
    28681 tactggagaa atatctggcg aagaccgaaa cgctggaaga gcagacgctg gaagaggcaa
    28741 tcgaagaaga gaaacagatc ccagtggata tttgcaacca tgcgctattg gtgggttacg
    28801 gtcgtgtagg cagcctgctg ggggagaaat tgctcgcctc tgatattccg ctggtggtga
    28861 ttgagacgtc acgaacccgt gttgatgagc tgcgagagcg tggggtccgc gctgtattgg
    28921 gcaatgcagc gaacgaagaa attatgcaac tggcacatct ggaatgtgca aaatggctga
    28981 tcctgacgat ccccaacggt tatgaagcgg gtgagattgt ggcgtctgcc cgcgcgaaaa
    29041 acccggatat tgagattatc gcccgcgccc attatgacga tgaagtgacg tatatcaccg
    29101 aacgtggcgc gaatcaggta gtgatgggtg aacgtgaaat tgcccgtact atgctggaac
    29161 tgctggaaac gccaccggcg ggtgaagtgg tgacggggta aacggtggtt gccggatgta
    29221 aaatatgaaa aatatgtagg catgataaga cgcgccagcg tcgcatcagg catcgagagc
    29281 caaatgccag atgcggcgtg aacgccttat ctggcctacg aaccgacgat aacttaacga
    29341 tcccagtaag actcttccag gctgtcttca cgctccggca agccgcgcgt taaacgaggt
    29401 gaatgctggt tcagtacctg atagctcaca cggttagcat atttacacac ctgcgctaac
    29461 gatgaataag ttaaccaggt gaatttatgt ttgctggagt ttggtatgtt gctacgatgg
    29521 tagctgttgg cggtaatgtc atgcagcaac gctgccaatg cgccatcccc cgctccatta
    29581 gtgttcatga ttttttccgg cccgcccatg tacggcgcaa tgtgcgaata gacacgcagc
    29641 ggattctggc aatccttgtg gcgcatggcg cggctaaact catactggtt gaattccgct
    29701 atagcgcccg gcagcagcgg atgctgggtt ttacgtttcg cttcgtcttc ggtaaagccc
    29761 gccatataca agccgattgg cccggcggtg cacagcacca gatctaccca gtccagcgcc
    29821 ttgtcagatg ccaacaacgg atcgctttct ccggtcaacg cttcggcttc atcttcgttc
    29881 atcgcaagga tagaaacgtg gtctttgagg aattgctgcc accactgcgg attctcggca
    29941 atgacaaact tggtgcccag cgtcagcacc accggtacgt tatatttctt cgcgtactca
    30001 atggctttca tggttgcttc cggcatgggt tcacccggct tgcaacgcac cagatatgag
    30061 gtgagaacca gtgccgaggc tccggcaatc acatcttccg gaatgctttc agcccgcagc
    30121 tggttcatgt ggcttggact gatagcaaag gtacgttccc cggactcgcc aatcagcgta
    30181 aagcaacgac caatcgggcc atccacgcct tgtagatagt taagatcggt acggctggaa
    30241 gtgttacaca ggtaacgata ggcataactg ccaatttcaa tattgctgca catgacgccc
    30301 agcagcaccg aacggtcgtc cgcgagcacc gagtagttgt gcatggtgtt accaatggtg
    30361 ccacccgcaa actgatgggt aatcagattt ttctgtttta gctcctgata aagcgcttcg
    30421 gctacgtcat cctcaatcac cagtgaatgc ccggcgctta atccataacg ctcaatgaat
    30481 tcatcatcca ctttcgcttc aatatcgacc agcgtctggt caatacctac gacccaggcg
    30541 gcgctggttt cgttttctgg ctggaattgc tgaagcagcg gatcgcgtgc atttacaggg
    30601 aagtaatgtt tggatttacg tttaccggga aatttcatgg ttgtttttac gggtggttaa
    30661 ctgagcggcg aatgttaaca caatctgacg ggagcgcgcg agacgcagcc agtttcaata
    30721 tcagcaggcg ggatcgcatt ttcatggcag tggttcctta caatgacgta atttgaaagg
    30781 agtttttgta tgaagccgga aaacaaacta cctgttctgg accttatttc tgctgaaatg
    30841 aagaccgttg tgaatactct tcagccggat ttaccgtcct ggcccgcaac gggaacgatt
    30901 gctgagcaac gacagtatta cacgcttgag cgccgattct ggaatgcggg cgctccagaa
    30961 atggcaacca gagcttacat ggttccaaca aaatatgggc aggtggaaac acgtctcttt
    31021 tgtccgcagc cagatagccc ggcgacgcta ttttatttgc atggaggcgg ttttattctc
    31081 ggcaatctcg atacccacga tcgcatcatg cgcctgctgg caagctacag ccaatgtacg
    31141 gtgattggta ttgattacac cctttcacct gaagcgcgtt ttccacaagc gatagaggaa
    31201 attgtggctg cttgttgtta tttccaccag caggcggagg attatcaaat caatatgtcc
    31261 cgcattggct ttgccggtga ttccgcaggt gccatgctgg cgctcgccag tgcgttgtgg
    31321 ttgcgtgata aacagatcga ttgcggtaaa attgcgggtg ttttgctgtg gtatgggctt
    31381 tacggattac gggattccgt gactcgtcgt ctgttgggcg gtgtctggga tggcttaacg
    31441 caacaggatt tgcagatgta tgaagaggcg tatttaagca acgacgcgga ccgcgagtca
    31501 ccgtattact gtctgtttaa taatgatctc actcgcgaag ttccgccctg ttttattgcc
    31561 ggggcggagt tcgatccgct gctggatgac agccgtctgc tttaccagac gttagcggcg
    31621 catcagcagc cctgtgagtt caaactctac ccaggtacgc tgcatgcctt tttgcattat
    31681 tcacggatga tgaaaaccgc cgacgaggct cttcgcgacg gcgctcagtt ctttaccgct
    31741 cagctttagc gatacgcggc aacaagatta gccatcattt cgatatgttc cggcgtggca
    31801 ttaagcgctg gaatatattc atattttttc ccgcccgcac cgaggaagac ctcacggttt
    31861 tgctcggcaa tctcttccag cgtctccaga caatccgcag caaagcccgg gcacatcacc
    31921 tgtatatgac ctacgccttt ttctccgagc attttcagcg cttcgtcggt ataaggcatc
    31981 agccagggtt cccggccaaa gcgcgactga aaggtcatca tcactttttc cggtgccatc
    32041 cccagtgcgg aagccagttc gcgagtcgtt gtgcggcaac gttgcgggta atcatcgcct
    32101 tcatctgcat aacgctgggg aatgccatga taagagagca gcagcagatc tggttcgcca
    32161 tgtttggcaa aagaagcgcg tacgctgttc gccagtgcat taatgtaatc gtggttatcg
    32221 gcgtaatcac gaataaacga tatccccgga atgctacgtt tgcgcgccag aatgcgtgcc
    32281 agttcatccc ataccgcacc gaccgtagaa caggagaatt gcggataaag cggcagcacc
    32341 acaatatgat ctacatgctc tgccaggagt tcatcgacgg cgctttccag tgatggcgag
    32401 ccgtagctca tgcccagcgc cactggcgta tccggtaaac gttgtgccaa cgcctgctgt
    32461 tgctggcggc tgtaaaccat cagcggcgag ccgtcttcca tccagacaga ggcgtacagc
    32521 ttcgccacac gcggcgagcg cagcggcaaa atcacgccgc gcagcaacgg ccaccataac
    32581 aaccgtgagg tatcaaccac gcgtctgtcg cttaaaaatt gtttcagata gcgttttacc
    32641 gcttcaggtg tgggggcatc gggcgtaccc aggtttgcca gcaggatacc ggttttagtc
    32701 tgacgcatta ccgcctctta tcgattcaac ttgttgataa ttgtagcgga aaagtgagga
    32761 agaagaacta attgctgtga gaggtattgc ctgaggtgga tgagcagacc tgcccgaaag
    32821 cagatctgct gcggcttgat tagccgagga ttttttccag atcagcgcga acttcagcaa
    32881 ccggcttggt accgtcaact ttcgcgtatt tggtgttacc cgcttccgct tctttggagt
    32941 agtagccgat cagcggtgca gtcatctgat ggtattcaac cagacgttta cgcacggttt
    33001 cttcctgatc gtctttacgg gtagtcagtt cttcaccggt aacgtcgtct ttgccttcta
    33061 ctttcggcgg attgaattta acgtgataaa cacgaccaga cggcgcatga acgcggcggc
    33121 ctacgatacg atcaacaatc agttcgtccg gtacgtcgaa ttccagaacg taatcaacat
    33181 tgatgcccgc ttctttcatc gcgtctgcct gcggaatggt acgcgggaag ccgtccaaca
    33241 ggaaaccatt acggcagtct tcctgagcaa tgcgctcttt aaccagcgcg atcaccagtt
    33301 cgtcggtgac cagtttgcca gcatccataa tgtcttttgc ttgtttaccc agctcggagc
    33361 cagatttgac cgcagcacgc agcatatcgc cagtggagat ttgcggaata ccatatttct
    33421 ccatgatgaa ctgagcctga gtccctttcc ccgcgcccgg agcgccaagc agaatgatac
    33481 gcattgcgaa aatcccctta aaatgtgtcg atttttttga aaaagcgata aacgatacca
    33541 ccaacaggcg attgcctcaa gaaaggcacc tgaggctgaa acggttaatg gcgagataat
    33601 ttgcggaaag tgcagatgat gaaaagaaaa atgccggatg acacaaaggt catccggcat
    33661 gacatcagga aaccagcagc tggttcatac gacgaataaa caggttcgga tcttccagcg
    33721 tgccgcgttc tgccagcagc gcctgatcca gcagcagttc tacccactcg ctgaacttgg
    33781 cttcatcttc agtatctgcc gcacgtttca ccagtacgtg atccgggttc agttcgaaga
    33841 tgtatttcac ttctggcact ttctggcccg ccgcagcgaa cagtttcgcc atctgagtgc
    33901 tcatttcgtc cgcgtcggtc gaaacgatcg ctggcgtatc ggtcagacgg tgagtcagac
    33961 ggacatcttt cacgcgctcg ccgagcagag ctttcacgcg gtcgatgaac ggagtcagtg
    34021 ctttctccgc ttctttcgcg ctctcatcaa cttcgtcagc cagtttttcc agcgactcgt
    34081 caactttaga caccgactgg aacggtttac cgtcgaactc agtcagatag ttcatcatcc
    34141 actcatcgat gcggtcggaa agcagcagaa cttcgatgcc tttcttacgc agcagttcca
    34201 ggtgcgggct gctcttcgct gccgcatagc tgtctgcagt gatgtagtag attttctcct
    34261 gcccttcttt catgcgggaa acgtagtctt ccagagacac ggtctgcgca gaagaatcgg
    34321 tataagtaga agcaaaacgc agcagtttgg cgatcgcttc ctggttagcg aaatcttccg
    34381 ccggaccttc tttcagtacc aggccaaact gttgccagaa ggtctggtat ttttccgcgt
    34441 cgtctttcgc cagtttttcc agcatttgca gcacacgctt ggtcagcgca ttgcgcaggt
    34501 tacgcgttac cgtgctgtcc tggaggattt cacgggaaac gttcagcggc agatcgctgg
    34561 agtcaatcag accacgcacg aagcgcagat agttcggcat gaactgttca gcgtcgtcca
    34621 tgataaacac gcgttgaacg tacagtttca ggccgtgttt atgatcacgg ttccacatat
    34681 cccacggagc ctgggacggg atgtacagca ggctggtgta ctcctgctta ccttcaacgc
    34741 ggttgtggct ccaggtcagc ggatcgttaa agtcgtgggc gatgtgtttg tagaactctt
    34801 tatactcttc atcggtgatt tccgacttgt tacgagtcca cagcgcctgc gctttgttga
    34861 tttttgccca ggagataacg gtttcgccat ctttctcttc gcgtttttcg atctctaccg
    34921 gcagcgcgat atggtcggag tatttgctga tgatggaacg cacgcgccag tcatcgagga
    34981 actcgtcttc gccttcacgc agatgcaggg tgatttcagt accacgatct tctttggtga
    35041 tgtcggcaac ggtatattca ccttcgccag ccgattccca gaagacgccg ttttctggtt
    35101 tttcgcctgc cgcgcgagta cgcacggtca ctttgtcggc cacgataaac gcagagtaga
    35161 aaccaacacc aaactgaccg atcagctggc tgtctttcgc ctggtcagaa cccagggatt
    35221 cgaggaatga tttggtaccg gatttagcga tagtccccag atggtcaatc acttcgtcgc
    35281 gggtcatccc cacgccgtta tcggagatgg tcagcgtgcg cttgtcttta tcgaaagaga
    35341 cacgaacgcg cagttcgcca tcaccttcgt acaggtccgg gttagatagc gcacggaaac
    35401 gcagcttgtc cgccgcatcg gaggcgttag agataagctc acgcaggaag atttctttat
    35461 tggaatagag agaatggatc atcaggtgca gaagctgttt cacttctgac tgaaaaccac
    35521 gagtttcttg tcctttcatg taggtctacc tcaataatgc cattttaagg ttaaaaacag
    35581 gatgagggag agatggggac aagggagaat aatttcaagc gagagcaggt gctcctgctc
    35641 tcgtttgctt aaaaacgaat cttatgacgc ccggcgaggg agtgtgacag cgtggtgcca
    35701 tcgaccattt ccagttcgcc gccaaccggt acgccatgag cgattcggct ggcttccacg
    35761 tcatattgcg cgcaaagctc ggcaatgtag ttagcggtag cttcaccttc aaccgtggga
    35821 ttggtggcga ggatcacttc agtgattttt tcctctgcca gacgctgttc cagacgatca
    35881 agcccgatat catccggacc gatgccgtcc agcggtgaca gatgccccat caacacaaaa
    35941 taacgaccag aaaactgccc cgtctgctca atggcgtaga tgtccgccgg actctccacc
    36001 acacagattt gaccgttttc ctgacgacgc ggattcgaac agatgttaca gacttcctgt
    36061 tcggtgaaag tgcggcaatc ggcgcagtgg ccgatttccg acatcgcccg ggtgagcgcc
    36121 tgcgccagac gcatcccgcc gctacgatcg cgctgaagca gcgtgaacgc catacgctgc
    36181 gccgacttcg ggccaacgcc cggcagacag cgcagtgctt ccataagctg tgttaacagc
    36241 gggctggttt gcatcagaac ggcatcttaa agccaggcgg cagctgcatt ccggaggata
    36301 cagaggccat tttttctttc tgcgtttctt caatacgacg tgctgcgtcg ttgaatgctg
    36361 cagccaccaa gtcttccagc atctctttgt cgtcttccag caggctcggg tcgatctcta
    36421 cgcgacggca gttgtgtgca ccgttgatgt tcacttttac cagacctgcg ccagattcgc
    36481 cggtaacttc cagctgcgcg atctcttcct gcattttctg cattttttct tgcatctgct
    36541 gggcttgctt catcaggtta cccagaccgc ctttaccaaa cataggtttc tctctcaatc
    36601 acgttaagga tgacgaacgt aagctgtgct tacgatcaaa tggggcggat actttcttca
    36661 tccagctccg catcgaaaaa ccgacgcagg gtctgaatat tattatccgc aataatggac
    36721 tcgcgcgcct gcgcaagttt ttcttcgtat atcgcctgac gccactccag cggcgtacgc
    36781 accgcgggat tatcatcttc aacgatagtc agttcaaccg ttgaaccttt taacatgctc
    36841 aacgcttcag ccagtttttg ctgtgcaccg cggttgttca aatgccgctg agaggagcgc
    36901 aaatgcagac atactgcgtt gtcgctctcc tctttccagg catttaacgc cacctgttcg
    36961 accagttttg gtagcgaaag ttggctcacc tgtgccgccc acgggtcgcg ctcaatggct
    37021 tctgccgcta gcttcgccgc cagttccggc gttttttcat gttccagcgc ttttttcagc
    37081 gccttcggcg tggcgaccac ttctttttgc tgcatcaccg gagtggtcgc cttccagcga
    37141 tacgcttctt ttttggctgg cgctttttcc agcgccgatg gcaccggacg cgcctgaacg
    37201 cgatcggtga ccgaagccag tctttccagc gcagcgttat tcaccggccg cgcgcgggta
    37261 gcggctgccg gttcactctt ttttgctttg gttgctccct gcacgcgctg caactgctgg
    37321 cgcgccgcca gcacctggct ggtggtttcc gggagcggta cagtcggtgc ctgctgcggc
    37381 gctgattgcg gcggcaccag ggttggcgtc attactgccg ttggcgcgac gggtgcaaag
    37441 gactggcgtg gcacttctgg ctcaggcagc ggcatacgcg gatggaacgc cagcgcgcgc
    37501 agcagcgtca tctcaacgcc catgcggcgg tccggcgcat acggtaattc tttgcgacca
    37561 atcaacagcg tctgatagta aagctgaata tccgtcggcg gtatggtgcg cgccagttca
    37621 cgcatccgca gctcgatggc ggccatgtcg ttgccaagtg cagcaggcga aagttgtacc
    37681 atcgcaatac gatgcaacaa gccgagcatt tccaccagca acgcttccca ctcgatacca
    37741 cgggcagcgg cttcattaat cagtgccatt acgcgctcgc cgttgccctc gaccatcgct
    37801 tcaaccagcg acagcgcctg atcgtcgtca agcgtaccca gcatcgcact gaccgcctgg
    37861 gttgaaacct ggccgtcacc gctggcaatc gcctggtcgg tcagacttaa ggcatctcgc
    37921 aggctgcctt cagcggcgcg cgccaacaat tgcagcgccc gcggctcgtg agcgatatgt
    37981 tcttcattga ggatgtgctc aagctgatgg cgaatttgct cgacatccag cgccttgaga
    38041 tgaaattgca gacagcgtga caaaatcgtc accggcaatt tctgtggatc ggtcgtcgcc
    38101 agcagaaact taacgtgctc cggcggctct tcaagggttt ttaacagtgc gttaaagctg
    38161 tggcgcgaca gcatatgcac ttcgtcgatc agataaactt tgaaacgacc acgcgccgga
    38221 gcgtactgga cgttatccag caggtcgcgg gtatcttcaa ctttggtgcg cgaggcggcg
    38281 tcgatttcaa tcagatcgac aaagcgcccc tgctcgattt cacgacagtt atcgcacacg
    38341 ccgcacggcg tcgcggtaat gccggtttcg cagtttagcc ccttcgccag cagtcgggcg
    38401 atagaggttt ttccgacgcc acgggtgccg gaaaaaagat aagcatgatg aatacgccct
    38461 aacgacaagc cgttcgccag tgcggtcagc acatgttcct ggccgacgac gtcagcaaag
    38521 gtttgtgggc gccattttcg ggctaaaacc tgataactca ttggcaggct ctgaaacgct
    38581 ggaaggtgga ttcacgaagg ggtaatgcta acacagcccc gtcagaacgg cgaggctgaa
    38641 tgcacatctt gtgctgtccg tagcgtgggc agcacaagac tggcgataat taatggcccg
    38701 ggaacgggac aaggctgtag ctggtaatgc cctgtttttc gagacgctgt tcgccgccga
    38761 gatcgaacag gttgataatg aacgcagcgt cagccacttc accacccaga cgacggatca
    38821 gtttaacggt cgcttcgata gtgccgccgg ttgccagcag atcgtccacc accagaactt
    38881 tgtcgcccgg tttgatggca tcaacgtgga tttccagctg atcggtgccg tattccaggt
    38941 cgtaattttc actgatggtt tcacgcggca gtttgcccgg tttacggacc ggtacaaagc
    39001 caacgcccag acccagagct accggagcgc caaacaagaa gccacgcgct tcggtgccga
    39061 caactttggt aatgcccgca tttttgtaac gctcaaccag caagtcgatg ctgagagcgt
    39121 aagctttcgg gtcttccagt aagctggtga catcgcggaa aagaatgccg ggttttgggt
    39181 agtcctgaat gcttttgatg ctatttttga gatactcaag ctgctgtgca gtcgcggtca
    39241 taagtgtatg cctgcttgtt acggtggtac tcacggcgcg ttttaaacgt atcaaaagta
    39301 actgttgttt aacctttgac cgcctgcgcc tgtgctcgaa aacgccagaa tgtactggct
    39361 gtacgcaata attgcaactg cgattgttgt gcttcagtgc ttttcttgct tttcatcaat
    39421 caccggaatt cgccacatat agaaaagcaa acaggcgaga attaccagca acatgatgcg
    39481 cacccatggc atctggacga accacagaga aatggcaaac gtgagcaaaa taagcaaaat
    39541 cgcccgcggt ttgacgccgc gcggcatcgc atgatgtttc tgccagaaac gtagatagct
    39601 gccaaaccat gagcggtaca gcaaccaggc gtgaaagcgc ggggaagaac gggcaaagca
    39661 ccaggccgcc agcaggataa acggcgtcgt cggtaatacc ggtaatacca cgcccagcgt
    39721 acccagcact accgccagcc agccaatgat gattaaaatg attcgttgca tattgagtgt
    39781 tgtcagctta cagagtggct actttagcat aacaattatc attttcattg aggtcttatc
    39841 gtgaaaaccg ccctgctgct ggaaaaactg gaaggacagc tcgctacgct gcgtcagcgt
    39901 tgtgccccgg tgtcacagtt cgccacgcta agtgctcgtt tcgacaggca tctttttcag
    39961 actcgtgcga caacactaca ggcttgtctc gacgaggcgg gcgataatct ggctgcgctt
    40021 cgtcatgcag ttgagcagca acagctgccg caagtggcct ggctggcgga acatctggcg
    40081 gcacaactgg aagccatcgc gcgtgaagcc tccgcctggt cattgcgcga gtgggacagt
    40141 gcgccaccga aaattgcccg ctggcagcgt aaacgtattc agcatcagga ttttgagcgg
    40201 cggctacgtg agatggttgc cgaacgcaga gcccgcctgg cgcgggtgac cgatctcgtg
    40261 gaacagcaaa cgctgcatcg tgaagtggaa gcctatgaag cgcgtctggc acgctgccgc
    40321 catgcgctgg aaaaaatcga aaacaggcta gcgcgcttaa cccgctagca atggagagag
    40381 tatgtcactt gaaaatgccc ctgacgatgt caaactggct gtcgatttga ttgtgctgct
    40441 ggaggaaaat cagatccctg ccagtaccgt gctgcgcgcg ctggatattg taaagcgtga
    40501 ttatgaaaag aaattaacgc acgatgatga ggcggaaaag tgagaaataa atggggccgc
    40561 taaatgtccc ctaagattgc gggcaaacgc aaaactgcct gatgcgctac gcttatcagg
    40621 cctacaccgc tcctgcaata tattgaattc ttgcggtttc gtaggccgga taaggcggtc
    40681 acgccgcatc cggcatgaac aacgcgcact ttgtcagtaa tctcggggca actaaatgtc
    40741 gccccctttg gttattaccc aaccgctggt gtcggatcgt cgcctttgta gtcgcgtttt
    40801 acttccgtca cctcatcgcc cttctcgttg tgcagatgca cttcaagctg gttaaaggca
    40861 atgttgatgt cgttttcacg gcacagctga tcgatagtac ggttcagctc atcgacagta
    40921 cggctacggt cacgcagttc acgcacatac agacgcagct catgatccaa cgtgctggca
    40981 ccaaatgccg taaagaagac ttccggcatt ggttcgtgca tcacccttgg gtgctcagtc
    41041 gccgccttca gtaacacttt acgcactttt tccagatcgg agccataggc cacgccgaga
    41101 cggatcacca gacgcgtagt agtgtcagtc aacgaccagt tgatcagacg ctcggtaaca
    41161 aacgctttgt tcgggatgat cacttctttg cgatcgaaat cggtaatcgt tgtcgcacga
    41221 atacggatct tacttaccgt ccccgagaag ctaccaatgg ttaccgtatc gccaatacgc
    41281 accggacgtt cgaacagaat gatcaaaccg gagacgaagt taccgaaaat ttcttgtaaa
    41341 ccaaaaccaa gacctacgga taatgctgcg gccagccact ggagtttatc ccaagagacg
    41401 cccagcgatc cgaacaccgt catcgcacca acggcaataa tgatgtagtt aaggatggta
    41461 gtaatggcat acgacgcgcc ctggcgcata ttcagtcgcg agagcaccag cacttccagt
    41521 aaaccaggca ggttgcgaat caacgcccag gccaccattg aggcgataat cgcaaacaac
    41581 agactgccca tggtgacgtt tttcaccacc gcagcgccag cttcagtgcc gttgtaatgc
    41641 cagagcgtga tgctgtcgag atagctgaac acggtgatca aatcggacca aattgcccag
    41701 aacatgacac cgaacagcgc aaacatcagc aacatggtaa tacgcagcgt ctgctggtta
    41761 acttgctcca gtgcaatggt gggttcttcc ggcggttcgg caccttctgc gccctctttc
    41821 accagattct gccgacgcgc cagcgcacga cgccaggcga tacgccgcgc cgctacgctt
    41881 aagccgcgca ataccgtctg gtagagcaag ttccagatga tcaccagata aacggtttca
    41941 atccaacgcc cggagaggcg cagcgtggtg tagaaataac ccgtggcagt cagcaccatc
    42001 aatgcaatcg ggataatcga cagcacggta atggtgacca gtcgcatggt gtgcgactct
    42061 ttatcacgcc agctttcgcg gcacattggc cataccagga aggcaatcag cagcaggttg
    42121 aagaaaatca tcgcctgccc cagcacatca tccatcagat gcagcgggga aagttctgcc
    42181 accacagacc agaaatggat aggcagcaac gcgagactga tgcggacaat ttgccgacgc
    42241 cagtggctgg tctgctgctc cggcatgccg aagtgacgca cggcaacgcc atttttctcc
    42301 agtaccttcc agcacaggcc aaacaccagc cagaaaatcg ccagtttttt actgaacgac
    42361 catagcagtt cgctgatgtt gagctgcatg gtcaacagaa tcaggccaac cgcgagaata
    42421 atcaggcaca ccggcagcgc gcggatcagg tcgataagga tcgcttttgg tgtgttgagc
    42481 tggctatcgt tacgcaggga acccacagcg gaagccagtt tctgttgata cgctttcagc
    42541 cagcccagac gccagtggat cagcccggca atcaacagca gcggcaaacc ggcgaggaaa
    42601 gcgataaaaa cggcaggcca ggctttttcc cagttcaccg tgattttcat cgacttaaat
    42661 tcatctttca ggctttgcgg gaacgctttg atccagtccc agtccattgg acggttactg
    42721 ttcacccaaa agatttgctg agtcaggatg gatttcaggt ttttcgatac gctcattaac
    42781 tgctgctggt tgatttgcag gttaatggcc atcatcagct ggttacccaa ctgtttgttg
    42841 agttgatcca gcaattcgcg acgcatatca accacttgca ataacgcatc gtgaacttcg
    42901 ctgttgactt cgttggtgtg accttcttcc agtttgttga cgaacgcatc gctctggaag
    42961 agtgcatcac gctgctggtt aacttcaaac tgttcgagac gcaaatccgc gatgcggttg
    43021 gtcatgtttt ccagttcatc cgccgagggc agcgtttgtt gttgctggta aaggatacga
    43081 gacaacagca ggctgccctt caggacggca atctgctctt taatattgcg ttccgattgc
    43141 agcgcccgct ccagccagtt tttgacttta atgttttgct gcatcaactg attaccgttt
    43201 tcagtcgcgg taatcagacg ctgacttaac tgctggttaa tttccagttc ctgtttcacc
    43261 agcggattag cctgaatacg cgcggcttca tccggggaga cggcttcctg cgccgttttt
    43321 tcagttaaag tcaggcgctt gctgtttacc gcttcttgca acagctgcaa ctggtgctcc
    43381 agacgggcgc tgttcgccgt cacgtaatca cgttgctttt gcaaggtatc ctgcaagacg
    43441 gtgttccctt ccaggctttt acgctgctgg tcaatctccg cattcagcaa cgcctgctga
    43501 gcctgcatta acactttctg gctgggacgt aaggcagtct cgccgacatc agtcccatcc
    43561 agacgactgc gaatttgttg cagctgctgc gaagcgttat acatcgcatt ttgcacgcgt
    43621 tcgggctgcg tctgtaacga aaccagctgg ctgttataag acgccagatc gttttgtgcg
    43681 ttttgcaaat cgtccagcgc ctgggcaacg cgagtttcca gctggcgcaa cgaaagcgta
    43741 ctcagaattt tgcgcgtttc ttcgtcgtta tcgacatcgc taagtgctgt taacgccgcg
    43801 gtcgcctggc gcattttttc cggcgcttca gcgacttttt gccgtagctg aactgtctct
    43861 tcttttatgc gatcgatttt atcgagggtg gctaatgtat ctgtcagatc ctgctgcacc
    43921 agtttgtcct gagcagaaag atctttttgt ttatttagtg agtcaagttg cgcctgcagg
    43981 tccgcttttg tcggcagatc accattcgat gacgcccgcg caaacgccgt gttctggcat
    44041 aacaagacaa aaacaaaagc aataaatgct gaaaaaacaa aatgccgtga tcgtttgtaa
    44101 tactggaaca tagtcatgat gaatgaaggt ttctgaacct gaagaacgac ctgaaaaagt
    44161 caaaccgcaa gaatatcacg acgcagtgaa ccagaatagc aacgacgaaa atgtccagga
    44221 aaaatcctgg agtcagattc agggttattc gttagtggca ggattacgaa gcgtggggca
    44281 caggagatac atctccagta agatggcgac gtaatcgcgg gcttcttttt taagatcaaa
    44341 agattgcggg gcaaagagcc agttttccat caggccggaa atatagccgc gcataataat
    44401 tgctgcccga cgcgtcatta aatccgcagg caacattttc gcttcaatac aatgttttaa
    44461 cgtttgttct atacggtcat aactttccag acagagatta cgttgtgcct gttgcacaac
    44521 agccatttct ccgacaaatt cgcatttgtg gaatataatc tccatcaata atcgacgtcg
    44581 ttcttctgtc accgtggatt caagaacatg aattaatatc tctcttaata ctgagagtgg
    44641 atcgccaggg aattttgcct gatactcaag ctctagttca ccaatattgg attctgacag
    44701 ttcccagatc tcactgaaca aatccgactt gtctttaaaa tgccagtaga ttgcaccgcg
    44761 cgtaacgcca gctgcttttg caatctcgcc cagcgaggtg gatgataccc cctgctgtga
    44821 gaaaagacgt agagccacat cgaggatgtg ttggcgcgtt tcttgcgctt cttgtttggt
    44881 ttttcgtgcc atatgttcgt gaatttacag gcgttagatt tacatacatt tgtgaatgta
    44941 tgtaccatag cacgacgata atataaacgc agcaatgggt ttattaactt ttgaccattg
    45001 accaatttga aatcggacac tcgaggttta catatgaaca aaaacagagg gtttacgcct
    45061 ctggcggtcg ttctgatgct ctcaggcagc ttagccctaa caggatgtga cgacaaacag
    45121 gcccaacaag gtggccagca gatgcccgcc gttggcgtag taacagtcaa aactgaacct
    45181 ctgcagatca caaccgagct tccgggtcgc accagtgcct accggatcgc agaagttcgt
    45241 cctcaagtta gcgggattat cctgaagcgt aatttcaaag aaggtagcga catcgaagct
    45301 ggtgtctctc tctatcagat tgatcctgcg acctatcagg cgacatacga cagtgcgaaa
    45361 ggtgatctgg cgaaagccca ggctgcagcc aatatcgcgc aattgacggt gaatcgttat
    45421 cagaaactgc tcggtactca gtacatcagt aagcaagagt acgatcaggc tctggctgat
    45481 gcgcaacagg cgaatgctgc ggtaactgcg gcgaaagctg ccgttgaaac tgcgcggatc
    45541 aatctggctt acaccaaagt cacctctccg attagcggtc gcattggtaa gtcgaacgtg
    45601 acggaaggcg cattggtaca gaacggtcag gcgactgcgc tggcaaccgt gcagcaactt
    45661 gatccgatct acgttgatgt gacccagtcc agcaacgact tcctgcgcct gaaacaggaa
    45721 ctggcgaatg gcacgctgaa acaagagaac ggcaaagcca aagtgtcgct gatcaccagt
    45781 gacggcatta agttcccgca ggacggtacg ctggaattct ctgacgttac cgttgatcag
    45841 accactgggt ctatcaccct acgcgctatc ttcccgaacc cggatcacac tctgctgccg
    45901 ggtatgttcg tgcgcgcacg tctggaagaa gggcttaatc caaacgctat tttagtcccg
    45961 caacagggcg taacccgtac gccgcgtggt gatgccaccg tactggtagt tggcgcggat
    46021 gacaaagtgg aaacccgtcc gatcgttgca agccaggcta ttggcgataa gtggctggtg
    46081 acagaaggtc tgaaagcagg cgatcgcgta gtaataagtg ggctgcagaa agtgcgtcct
    46141 ggtgtccagg taaaagcaca agaagttacc gctgataata accagcaagc cgcaagcggt
    46201 gctcagcctg aacagtccaa gtcttaactt aaacaggagc cgttaagaca tgcctaattt
    46261 ctttatcgat cgcccgattt ttgcgtgggt gatcgccatt atcatcatgt tggcaggggg
    46321 gctggcgatc ctcaaactgc cggtggcgca atatcctacg attgcaccgc cggcagtaac
    46381 gatctccgcc tcctaccccg gcgctgatgc gaaaacagtg caggacacgg tgacacaggt
    46441 tatcgaacag aatatgaacg gtatcgataa cctgatgtac atgtcctcta acagtgactc
    46501 cacgggtacc gtgcagatca ccctgacctt tgagtctggt actgatgcgg atatcgcgca
    46561 ggttcaggtg cagaacaaac tgcagctggc gatgccgttg ctgccgcaag aagttcagca
    46621 gcaaggggtg agcgttgaga aatcatccag cagcttcctg atggttgtcg gcgttatcaa
    46681 caccgatggc accatgacgc aggaggatat ctctgactac gtagcggcga atatgaaaga
    46741 tgccatcagc cgtacgtcgg gcgtgggtga tgttcagttg ttcggttcac agtacgcgat
    46801 gcgtatctgg atgaacccga atgagctgaa caaattccag ctaacgccgg ttgatgtcat
    46861 taccgccatc aaagcgcaga acgcccaggt tgcggcgggt cagctcggtg gtacgccgcc
    46921 ggtgaaaggc caacagctta acgcctctat tattgctcag acgcgtctga cctctactga
    46981 agagttcggc aaaatcctgc tgaaagtgaa tcaggatggt tcccgcgtac tgctgcgtga
    47041 tgtggcgaag attgagctgg gtggtgagaa ctacgacatc atcgcagagt ttaacggcca
    47101 accggcttcc ggtctgggga tcaagctggc gaccggtgca aacgcgctgg ataccgctgc
    47161 ggcaatccgt gctgaactgg cgaagatgga accgttcttc ccgtcgggtc tgaaaattgt
    47221 ttacccgtac gacaccacgc cgttcgtgaa aatctctatt cacgaagtgg ttaaaacgct
    47281 ggtcgaagcg atcatcctcg tgttcctggt tatgtatctg ttcctgcaga acttccgcgc
    47341 gacgttgatt ccgaccattg ccgtaccggt ggtattgctc gggacctttg ccgtccttgc
    47401 cgcctttggc ttctcgataa acacgctaac aatgttcggg atggtgctcg ccatcggctt
    47461 gttggtggat gacgctatcg ttgtggtaga aaacgtggag cgtgttatgg cggaagaagg
    47521 tttgccgcca aaagaagcta cccgtaagtc gatggggcag attcagggcg ctctggtcgg
    47581 tatcgcgatg gtactgtcgg cggtattcgt accgatggcc ttctttggcg gttctactgg
    47641 tgctatctat cgtcagttct ctattaccat tgtttcagca atggcgctgt cggtactggt
    47701 agcgttgatc ctgactccgg ctctttgtgc caccatgctg aaaccgattg ccaaaggcga
    47761 tcacggggaa ggtaaaaaag gcttcttcgg ctggtttaac cgcatgttcg agaagagcac
    47821 gcaccactac accgacagcg taggcggtat tctgcgcagt acggggcgtt acctggtgct
    47881 gtatctgatc atcgtggtcg gcatggccta tctgttcgtg cgtctgccaa gctccttctt
    47941 gccagatgag gaccagggcg tatttatgac catggttcag ctgccagcag gtgcaacgca
    48001 ggaacgtacg cagaaagtgc tcaatgaggt aacgcattac tatctgacca aagaaaagaa
    48061 caacgttgag tcggtgttcg ccgttaacgg cttcggcttt gcgggacgtg gtcagaatac
    48121 cggtattgcg ttcgtttcct tgaaggactg ggccgatcgt ccgggcgaag aaaacaaagt
    48181 tgaagcgatt accatgcgtg caacacgcgc tttctcgcaa atcaaagatg cgatggtttt
    48241 cgcctttaac ctgcccgcaa tcgtggaact gggtactgca accggctttg actttgagct
    48301 gattgaccag gctggccttg gtcacgaaaa actgactcag gcgcgtaacc agctgcttgc
    48361 agaagcagcg aagcaccctg atatgctgac cagcgtacgt ccaaacggtc tggaagatac
    48421 cccgcagttt aagattgata tcgaccagga aaaagcgcag gcgctgggtg tttctatcaa
    48481 cgacattaac accactctgg gcgctgcatg gggcggtagc tatgtgaacg actttatcga
    48541 ccgcggtcgt gtgaagaaag tttacgtcat gtcagaagcg aaataccgta tgctgccgga
    48601 tgatatcggc gactggtatg ttcgtgctgc tgatggtcag atggtgccat tctcggcgtt
    48661 ctcctcttct cgttgggagt acggttcgcc gcgtctggaa cgttacaacg gcctgccatc
    48721 catggaaatc ttaggccagg cggcaccggg taaaagtacc ggtgaagcaa tggagctgat
    48781 ggaacaactg gcgagcaaac tgcctaccgg tgttggctat gactggacgg ggatgtccta
    48841 tcaggaacgt ctctccggca accaggcacc ttcactgtac gcgatttcgt tgattgtcgt
    48901 attcctgtgt ctggcggcgc tgtacgagag ctggtcgatt ccgttctccg ttatgctggt
    48961 cgttccgctg ggggttatcg gtgcgttgct ggctgccacc ttccgtggcc tgaccaatga
    49021 cgtttacttc caggtaggac tgctcacaac cattgggttg tcggcgaaga acgcgatact
    49081 tatcgtcgaa ttcgccaacg acttgatgga taaagaaggt aaaggtctga ttgaagcgac
    49141 gcttgatgcg gtgcggatgc gtttacgtcc gatcctgatg acctcgctgg cgtttatcct
    49201 cggcgttatg ccgctggtta tcagtactgg tgctggttcc ggcgcgcaga acgcagtagg
    49261 taccggtgta atgggcggga tggtgaccgc aacggtactg gcaatcttct tcgttccggt
    49321 attctttgtg gtggttcgcc gccgctttag ccgcaagaat gaagatatcg agcacagcca
    49381 tactgtcgat catcattgat acaacatgta atcactaagg ccgcgtaagc ggcctttttt
    49441 atgcataacc tacgaacatt aaggagtaat tgaaccacca actcaggatc tcatacgaaa
    49501 accagtatta accacgtata aaattcataa aaaatactga ttgttagtta atttatatta
    49561 agtagcgcta atagatttaa taatccataa tcatttagag gctattctta attatttgcg
    49621 gtaattcttt attcattcct cggttattac gtcatattca gagcaatcct ggtattagtg
    49681 tcaccaattt catctggcga taatcctgaa atgttatgaa cagttcgagc aaactgcttt
    49741 tacctgctgc gggttagtgc tagtatgaaa aagtgagtcc tgtcccgatt ccttcctaat
    49801 tgtaattttt cgtaataatg cgatgaaaac ctgcaaagag tggcttatag ttaagctaac
    49861 aaacgagagg gcaagtccaa gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt
    49921 caaccactaa gaaggggacg cgttatggat gaatactcac ccaaaagaca tgatatcgca
    49981 cagcttaagt ttctctgtga aaccctgtat catgactgtc ttgcaaacct tgaagaaagc
    50041 aatcatggct gggttaacga cccaacctcg gcgatcaacc tccagttgaa tgaactgatt
    50101 gagcatattg cgaccttcgc acttaattac aaaattaagt ataatgaaga caataagctc
    50161 attgagcaga tcgacgaata tctggatgac acctttatgt tgttcagcag ttatggtatt
    50221 aatatgcagg ctcttcagaa atggcggaag ttaggtaatc gactattccg ttgttttgtc
    50281 aatgcgacga aagagaatcc tgcgagttta tcttgttaga attattacaa ccatgggtag
    50341 aagtatgtcc gaaaaacctt taacaaaaac cgattattta atgcgtttac gtcgttgcca
    50401 gacaattgac acgctggagc gtgttatcga gaaaaataaa tacgaattat cagataatga
    50461 actggcggta ttttactcag ccgcagatca ccgcctcgcc gaattgacca tgaataaact
    50521 gtacgacaag atcccttcct cagtatggaa atttattcgc taataaataa ttcgctttcg
    50581 gagctataac cggctgttta ttaagaattt tatacttttt cgccatgaag acatacccta
    50641 tgtgatcttt atcacacaga tgtaatgaga acgttctctt cactgacttt tcgtcttact
    50701 gtgttgccgc attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat
    50761 tgctggtgag ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg
    50821 tcagcttatt caccgataca atcattccct ggcggaagag cacacattac gccagcaaat
    50881 tctcgctgat ttattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga
    50941 ttatggctat aacatttttc tcggcaataa ttttttcgcc aacttcgatt gcgtgatgct
    51001 tgatgtctgc cctattcgca tcggtgataa ctgtatgttg gcactaggcg ttcatatcta
    51061 cacggcaaca catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc
    51121 cgtcaccatc ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac
    51181 cattggtgat aacgtcgtgg tagcttcagg tgcagttgtc acaaaaggtg tcccggacaa
    51241 cgttgtcgtg ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa
    51301 ctgttatgca aaattgtggt agatctgtta cttcccctct actattccca cgttaaaata
    51361 gggtgttccc tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc
    51421 tgctgaccga aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct
    51481 ttagtatcag ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt
    51541 ttgccaccct ggcggtgatg ttgcagtccg aaacgctgtc aggctctgtc tggctactgg
    51601 ttgtattatt tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct
    51661 atgaagatat cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc
    51721 gctttgtacc tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc
    51781 ataaggaaca actgcaaaaa atgatcgtcc gtaaaggtga actgtctttt tacgatattt
    51841 ttaccctcgc tcgcgccgaa tcaacatctt aagttagggt tacataccag gcgtaaagct
    51901 ctgcgcctgg tcaaatgaca atgatcgttt ccacccatca cttcatgaaa taccagctct
    51961 acctccttat ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt
    52021 aataaggata tgctggtgag aacacgacat ctggtcggcc ttatttcggg agtactgatt
    52081 ctttcagtat tgctgcctgt cggcttaagc atctggctgg cccatcagca ggtagaaaca
    52141 tcgtttattg aagagctgga tacctattcc tcccgcgtcg ctattcgagc caataaggtg
    52201 gcgacacaag ggaaagatgc gctgcaggag ctggaaagat ggcaaggcgc tgcctgtagc
    52261 gaagcccatc tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg
    52321 gtttatatcg ataacaacgt tcccctgtgt tcgtctctgg agcatgaaag tccgcccgat
    52381 acctttcccg agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcgcat
    52441 aacgatttag gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg
    52501 atcgaccccg cttcctttat tgatgtcatt ccctatagct catgacaaat tgatgccgcc
    52561 attattggca atgcccataa cgttgtcata accagcagcg atgaaattgc tcagggaatt
    52621 attaccaggc tacaaaaaac acccggtgag catatcgaaa ataatgggat catttacgat
    52681 atcctgccct tcccggagat gaatatttcg atcatcacct gggcttcaac gaaaatgttg
    52741 cagaaaggct ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggcctg
    52801 ctggcagcga tgtttgtgct gcgtattttg cgccgtatcc agtcaccgca tcatcggctg
    52861 caggatgcta tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc
    52921 aatggcaaaa ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggtagttgg
    52981 ttgtcaccag atagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg
    53041 ctactgatta taagaagcgt ctttgaagat atgggcgact gcttgcgtca gcatccacag
    53101 cagcatattt cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg
    53161 ctgcgtgaaa tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact
    53221 gaacgcgagt ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg
    53281 ggccatgaaa tttatcttga tgattttggt acggggtatt caagtttaag ttatttacag
    53341 gatctggatg tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa
    53401 aatgtcacgc cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag
    53461 ggaatcgaaa ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag
    53521 ggctggctct acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat
    53581 ttgtgaattt ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg
    53641 attgactcga atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac
    53701 tctttcatcc gtgggtccga catcatcttt tgattagctg cgtcgcggac ctcttttgaa
    53761 gggtattcaa tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga
    53821 aaatcggtca ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg
    53881 ccaaactctt taaacaatgg cgcggcctta gcggccattt cccgataggc atccttttta
    53941 tcggcaggaa cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg
    54001 tactgcgcag ccagacaatt cttgcgacgt cagttgtggt taaagtttag ccatgctggg
    54061 attttttgac gaaacggtgg gaggcagaaa tgggtggggg ccctgccagc tacatcccgg
    54121 cacacgcgtc atctgccttg gctgcttcct tccggacctg acctggtaaa cagagtagcg
    54181 ttgcgggaga accaacagag cccccattga gagcgttgaa aaccaacgcg caggcgcatt
    54241 atcactgctg ccccggctaa ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa
    54301 tgcgacttca ctcgggcgtt tttccagatt atgctgaaaa attacctcag gaagaaaaga
    54361 tggaaaaaga agattcattt ccccaacgcg tctggcaaat cgtcgccgct attcccgaag
    54421 gctatgtcac cacttacggt gatgtggcga aactggcggg atcgccccgc gccgcgcgcc
    54481 aggtgggcgg tgtgttaaag cgtctccctg aaggcagcac cttaccctgg caccgggtgg
    54541 ttaatcgcca tggcacaatt tcgctaaccg ggccggattt acagcgtcag cgacaggcat
    54601 tactggcaga aggtgtgatg gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct
    54661 ggaactacta accctctccg ggcggagagg gttaacgtcg cttaatattg cgtaggagcg
    54721 ggaactgccg aagacggatt cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg
    54781 ccaccgctgg cctgaaccgg cacggcggtt tgctgtaccg gcaccaatgt caggtcggct
    54841 ttagttccgc cctggttgat caccggctga acggtatcgg tgataaatac cagtttgtca
    54901 ttcacgttaa tcgccgcact caacagaata cgcgcgttcg gctgaacatc tgccgggtta
    54961 aatggcagaa caaagctgaa tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc
    55021 agcacttttg acggtgcatc ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca
    55081 tcaggcggca gtgcgacttt ctgacggatc cagacggtac cggagacatt cggttgctgg
    55141 atagcaggtt gttgtgttgc tgaaatagac gtatttgcag ccggggctgg cgtctgaata
    55201 tccgcgcttt tatctgcgca agccgccaac gcaatcgcaa ccgctaaacc actggccatg
    55261 tgcacgagtt tcattcattt ctccttatta tcaatgcacc agcgggctaa ctttcctcgc
    55321 cggaagagtg gttaacaaaa tagtaacgtc aacaagtgtg gcacacatca cgcatttctg
    55381 cctgtaatta gcccgtaatt cagactattg cacccatcgg accagtggca aatttgcgtt
    55441 atactcaact cactttggct tgctgcggca gctttgttac tggagagtta tatgagtcag
    55501 gcgctaaaaa atttactgac attgttaaat ctggaaaaaa ttgaggaagg actctttcgc
    55561 ggccagagtg aagatttagg tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc
    55621 ttgtatgctg caaaagagac cgtcccggaa gagcggctgg tacattcgtt tcacagctac
    55681 tttcttcgcc caggcgatag taagaaaccg attatttatg atgtcgaaac gctgcgtgac
    55741 ggtaacagct tcagcgcccg ccgggttgct gctattcaaa acggcaaacc gattttttat
    55801 atgacggcct ctttccaggc accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc
    55861 gcgccagcgc ctgatggcct gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg
    55921 ctgccgccag tgcttaaaga taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg
    55981 gagtttcata acccactgaa aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc
    56041 gcaaatggta gcgtgccgga tgacctgcgc gttcatcagt atctgctcgg ttacgcttct
    56101 gatcttaact tcctgccggt agctctacag ccgcactgca tcggttttct cgaaccgggg
    56161 attcagattg ccaccattga ccattccatg tggttccatc gcccgtttaa tttgaatgaa
    56221 tggctgctgt atagcgtgga gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt
    56281 gagttttata cccaagacgg cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt
    56341 aatcacaatt aaaaaaatag ccggaggtga aaaccgtccg gctatttttt gcagtgcttg
    56401 ttacgcgtta taggcattct cgccgtggct gttgacgtcc agcccttctc gctcttgttc
    56461 ttccggcaca cgtaggccaa ccgtcagatc cgccagtttg tagccaataa atgccaccac
    56521 accggaccag acgatcgtaa tggcgatgct ttccaactgt accagcaact gatggcccat
    56581 cgtcacacct tcagcgaagc ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat
    56641 gatacagccg acaatgccac aaacgccgtg cacaccgaag acatcgcagg gatcatccac
    56701 ccgcagcaag cgtttgagca tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc
    56761 gataatcaac gcgccgccaa ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc
    56821 ggcaatcgcg ccagaacacg ctcccagcag agaaggctta ccacgcagcg cccattcacc
    56881 gaagatccag ccaagaattg ccgcctccgt tgcgaccaca gtattcacaa atgccagtgc
    56941 cgcgatttca ttcgccgtgc ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag
    57001 aatggcagtc ccggtgaaga ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa
    57061 gcccacgcgt ttgcctatca gatacgcgcc caccagaccg gcgattgctg cgttaatgtg
    57121 caccaccgtg ccacccgcga aatccagcgc accgtgagaa gccagcaaac caccgcccca
    57181 caccatatgc gcaatcggaa tgtaagagag cgtcagccat accaccacga aaatcaacac
    57241 agctgagaag cggattcgtt ccgccagcgc cccaactatc aagcctacgg taatgcaggc
    57301 aaacgatccc tgaaacgcta cgtggatata ctgataaatg ctgcccatca ccgccgtcag
    57361 ttcgatgttt tttagcatca accagttaat gttgccgaag aagttgttgc cctcaccaaa
    57421 cgccagcgaa taaccgtaaa ccacccagag aatacagacc agtgcaaacg tcaccgtcac
    57481 ctgcgtcagc atcgacagca cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat
    57541 ccccggaata gtcataaaca gcaccagcgc agtacaaatc atcataaacg cattgtcggc
    57601 tttatcggcc accgcaggtg cagccattac cagtcccggc agcatcgcca gtgaagcaag
    57661 cccagttttt atcgtcgcta tcttcatttt tcgttcctgt tgctgtgtgc cagagattac
    57721 agcgccgctt cgtcggcttc gccggtacga atacgaatga cgcgttgcaa ttcagcgacg
    57781 aagattttgc cgtcgccaat ttttccggtg taagccgcct tactgacgat atcgatcact
    57841 tcatcgagtt ggtcatcagc aatcgccaca tcaattttta cttttggcag gaagttgacg
    57901 ctgtattccg ccccccggta cagctcggca tgccctttct gacgcccgaa acctttcact
    57961 tcggtgacgg tcaggccctg aataccaatg gaagataacg cttcacgaac gtcttccagc
    58021 ttgaatggtt tgattatcac ggtcaccagc ttcatagatc ccctccggtc agaattcggt
    58081 aatggttctg ctacacgaag aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa
    58141 agagagcagg aagttaagaa gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt
    58201 tacatttttg caccaacgat gaaatattgc gctattcagg cgctcaatga ttcctcttca
    58261 cgcacgctgg ctgccagctc ttcgcccgca agttgcagtt gatacatctg ccagtagcgt
    58321 ccctgggccg ccagcagttg ctggtgagtg ccctgctcca cggcttgccc acgatgaagc
    58381 accagaatgg tgtcggcatc aacaatagtt gataagcggt gagcaatcac caccagcgtg
    58441 gtatgttcac gcaccgccgc cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg
    58501 ctggcggttg cctcatcaag gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt
    58561 gccagcagtt gcttttgccc gactgagaga ttatttccct gctcgcccag cggcgtataa
    58621 ataccatcac tcatgctgcg cgccagctcc gccagttgca cggtttccag cgcctgccag
    58681 acgcgttctt cggagatatc ccgccccagc gtcacgttgg cgaggaaggt atccgccagc
    58741 accaccggat cttgctgcac cattgccacg ccctggcgca gcgcgctatg acttagcgaa
    58801 cttaatggac gaccatcaag gtgaatctca ccttccgtta gcgggtaata gcccatcaat
    58861 aaactggcga gggtactttt accactgccg gtatgcccga ccagcgccac aaagttacgc
    58921 gaaggcacag agagattaat gttctttagc accagattgt catcgcgata agcaaatgac
    58981 acgttatcga cttcgatggt gccactctgt aacgggtaat catcattgcc atattgctgg
    59041 cgcggtccgt ccatcagttc aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc
    59101 gcctgttgcg tggtcagttc gattaagggt tcgttaagtc gcccaagata gctgataaac
    59161 gcatacaaca cgcccacttc aatggtgccg ctggcggaga agccaaacag catcaataag
    59221 ccacaaagaa tgagcgatga aaacagactc agcagcggac gcagcagaaa accgtcgagg
    59281 cgcagggttt gcatcctcgc catatagtgt gaacgactgg cctcccccat acgttcgcca
    59341 aatcgcgcct gctgacgaaa ctgctggata acgctcatgc cattgatgat ttcgttaaag
    59401 ccgtcgttga tatccgccag ataggcgcgc acacggcgga caatcggcgt gctgtaacgc
    59461 tggtatatca ccattaccac cagcaccacc gggaaaatca ttatcgccac cagcgccatt
    59521 cgccagtcga ggctgaacat cgccaccagc atcgcgccca ccagtgcggc actgcgcagg
    59581 acagttgcca ctacggtaac gtagagatcg cggatcactt cagtgtcatt agtgacgcgg
    59641 gaaatcacct gcccgacggg ttgggtatca aactcgctta atggctggcg taacgcagca
    59701 tccatcacgt cggtacgcaa ctgttgcact acgcctactg ccgcccgatt aaacagcagc
    59761 gactgcgcgt aatgtagccc ggcggcaaac agttgtagcc caacatacgc cgcagccagc
    59821 cctgcaacca ctttcaacgg caggttattt ttcgctacca tattgtcgat aaaatagctg
    59881 ataagcagcg gcccactgac ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc
    59941 cccagcggtt tacgccacgg cgaaccatac gctaacaggc gcttgagagt cggccacagt
    60001 tggctaaaac tacgcatcga cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc
    60061 ctccagttgt tgatagcgat acatatcgcg ataccagccg ctttgttgcg ccagctcatc
    60121 atgattgcca cgctgggcga tatgtccgtg ctgcatcaca ataatttcac tggcttccgt
    60181 cagtgccgaa aggcgatggg cactgatgat taccgttctt ccctgtcccc actgacgcag
    60241 gttatgcagg atctggtgct ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat
    60301 gaggatttcc gcgttgacta ataacgctcg agcaatggag atacgctgtt tttgcccacc
    60361 ggaaagcatc acgccgcgct cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat
    60421 atcgtcatgt acgctggcta accgcgcgac atgctcaatc tcttgctggg tggcattcgg
    60481 gcaacccagc gcgatgttat tcgccacggt gtcagaaaaa aggaatggcg tctggctgac
    60541 caccgccagg cggctacgcc agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa
    60601 gcgaatatcc ccctcgctga cgtcgaaatg acgctgaatg agcgacaaca gggtactttt
    60661 gccggaacca gtcggcccgc aaatacccag catctgaccg ggtttcagga cgaaattgac
    60721 gttttccagc gcaggatggt cagtctgcgg atacgtgaac tggtgaatat ttacatccag
    60781 ttcgccacgc ccttccggca cgggttcgct accatcgttc accaccggcg cttccgccag
    60841 catcgcgcga atacggctgt atgcagcact accacgttcc acaatgttaa acatccatgc
    60901 cagcgccagc attggccaaa tcatcagacc taaatacatc ataaaactgg tgagctggcc
    60961 cagcgttaaa ctgccctgca ccaccatcca actaccgccg ccaatcgcca gcaagttcgc
    61021 cataccaatc gcgatataga tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat
    61081 gttttttttg ccggtatctt tggcatccac ggcaaataac gccgactggc gatcttccag
    61141 accaaaggct ttgatcatgc ggatactggt gaggctttcc tgggtgcggt cattaagact
    61201 ggaaaacgcc gcctgtgcca gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat
    61261 catgatcgcc atcactggca tcggtaacag ggcaaataag gtcaactgcc agctaatttg
    61321 cgtagacatc ataatcaaca cagcgcagcc cattaccagt gaatccacca gcgtcagcac
    61381 cccttctccg gcggcaaaca cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc
    61441 acccgtgcga tgacgcaggt aaaactccgg gtgctgccgg cttagctgac ggtaataatc
    61501 ttcacgcagt tcaacagcca gttggtaaga cgcgccaaac agcaatacgc gccagacata
    61561 acgcaggaga taaaccacaa cggcaatcag caccatggtg gcgatccaca tcaggatcag
    61621 cccggtagta aagtgttgtt ctgtcacgcc atcgacaaca ataccaacca cttttggcgg
    61681 aaccagttgc agcatcgcga taatgacaag caaggcgaca gccccaagat agcgacgcca
    61741 ttcccgacgg aaataccagc ttaattgagc aaataatcgc acgcgttgtg tcctgacctg
    61801 attctggata tttattcgat gggtaaagaa gtggtgtatt taatctgttc catcgcgaag
    61861 ctggaagtga cgtccgacag ccccggcacg ctgtttacca gacgcttata aaactcgtcg
    61921 tagcgtttca tgtcggcaac ctggacgcgc atcagataat cgtattcacc agccatgcgc
    61981 cagaacccca gcacttctgg catttcggta accaccgtga caaagcggca ataccattcg
    62041 ctgctgtgat gttgcgtttt tatcagcaca aaggcggtca ggccgaggcc tattttttcc
    62101 ggatccagca gggcgacttt gccgataagg ataccgtcgt cctccagccg tttcaggcgc
    62161 ttccagcagg gggtggttgt cagattaacg gcttcagcca gtgcctgcaa agagagggtg
    62221 caatcctgct gcagtaaggc cagcagctta cggtcaattt tatctaacat agcccttcca
    62281 cggagaattt ttttctcttt taattctatt ttaaaggtga aatcgcgaca atttattctg
    62341 tgcggtttaa acaccacggc acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt
    62401 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    62461 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    62521 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
    62581 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    62641 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
    62701 tcgaggcaat acgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    62761 tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
    62821 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
    62881 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
    62941 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    63001 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    63061 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    63121 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    63181 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    63241 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    63301 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
    63361 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
    63421 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    63481 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    63541 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
    63601 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaagtgag ctgcgcactc
    63661 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
    63721 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
    63781 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
    63841 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
    63901 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    63961 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
    64021 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
    64081 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
    64141 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
    64201 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    64261 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    64321 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    64381 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    64441 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    64501 tactgccagg ctggactatt ccgcactggc aagtaccgga tgaagtcaaa ctaccgaaaa
    64561 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
    64621 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
    64681 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    64741 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
    64801 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
    64861 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    64921 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    64981 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    65041 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    65101 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    65161 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    65221 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    65281 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    65341 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    65401 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    65461 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    65521 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    65581 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    65641 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    65701 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    65761 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    65821 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    65881 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    65941 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    66001 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    66061 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    66121 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    66181 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    66241 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    66301 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    66361 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
    66421 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    66481 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    66541 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    66601 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    66661 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    66721 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    66781 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    66841 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    66901 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    66961 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    67021 atccagcgcc agcagaacca cattaccttg catatcggtc gccataccgt agctcggttt
    67081 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    67141 gcttaaggtt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    67201 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    67261 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    67321 cggtttgtgc tcgctgatgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    67381 agagttgatg cccggcgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    67441 aaccggcttg aagttcaact cttccggcag gttatctttg ctgaaccaac ccgtctgagt
    67501 ggctttaacg ccggcagctt gctctgcacc ggccagagac tcggtgtcgt tgcttgccgc
    67561 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    67621 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    67681 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt ggcctttttc
    67741 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    67801 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    67861 accgccttta ttcagctcat caagtaccgc tttcgcttca tcttcagttt tggtctggat
    67921 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    67981 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcaaccagct tgatgtaact
    68041 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    68101 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    68161 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    68221 atcggtaccg gcaacgccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    68281 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    68341 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    68401 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    68461 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    68521 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    68581 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    68641 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    68701 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    68761 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    68821 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    68881 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    68941 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcggcagc gatggtgatc
    69001 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    69061 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    69121 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    69181 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    69241 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    69301 ttaataagga caagcccgat ccgctatcta acttagcgag acaaaaaaag gctggcaagc
    69361 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    69421 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    69481 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    69541 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    69601 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    69661 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    69721 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    69781 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    69841 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    69901 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    69961 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    70021 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    70081 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    70141 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    70201 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    70261 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    70321 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    70381 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    70441 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    70501 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    70561 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    70621 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    70681 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    70741 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    70801 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    70861 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    70921 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    70981 cttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    71041 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    71101 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    71161 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    71221 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    71281 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    71341 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    71401 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    71461 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    71521 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    71581 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    71641 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    71701 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    71761 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    71821 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    71881 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttggtta actaattgta
    71941 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    72001 acggtttgct ttgaccatca attaccgact cgtcgataac cactttttcg acgtcttcca
    72061 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    72121 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    72181 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcatttt
    72241 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgctacaa
    72301 ccggcagacg accaatgaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    72361 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    72421 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    72481 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    72541 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    72601 cctgctgtac tccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    72661 aaatcttgtc gatttcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    72721 cgcacttttg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    72781 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    72841 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    72901 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    72961 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    73021 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    73081 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    73141 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    73201 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    73261 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgcccta
    73321 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    73381 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    73441 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    73501 aactttcaga atttcacggg catgaatttc gatatcggtc gcctggccct ggtagccgcc
    73561 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    73621 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    73681 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    73741 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    73801 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    73861 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    73921 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    73981 cgtctcctgg ataaaattga aaaaaacctg ctgtaccgat tgtaacctta tggacggatt
    74041 atcagctagt ccctgtgtta tgggtacggc atcaccatat ttcaagcata acaatctttt
    74101 gttgttacgc taacaccgaa agaggctttt cgcacggctc catgcaaaat tcatgacaaa
    74161 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    74221 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    74281 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    74341 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    74401 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    74461 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    74521 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    74581 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    74641 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    74701 catattttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    74761 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    74821 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    74881 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    74941 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    75001 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    75061 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    75121 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    75181 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    75241 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    75301 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    75361 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    75421 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    75481 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    75541 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    75601 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    75661 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    75721 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    75781 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    75841 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    75901 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    75961 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    76021 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    76081 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    76141 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    76201 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    76261 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    76321 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    76381 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    76441 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    76501 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    76561 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    76621 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    76681 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    76741 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    76801 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    76861 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    76921 atcgtgccag ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    76981 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    77041 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    77101 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    77161 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    77221 tggatgacgg tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag
    77281 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    77341 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    77401 atgggttttc tcgaaccagg cacccaactc cgctggatgg cggcgctggc ggtggtgatc
    77461 gctttttgct ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    77521 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    77581 gtttccggcg gcctagccct gtggctggca gataaatggt tgggctggca gggcatgtac
    77641 tggttgatgg cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaacca
    77701 accgacacca ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    77761 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    77821 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    77881 ggtgaagtag gcgtggtaaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg
    77941 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    78001 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    78061 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    78121 gtcgcgctgt taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc
    78181 tcagcgcttt ccgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa
    78241 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    78301 ttgctgctgg tttgccgcca gacgcttgaa tatacacgag taaatgacaa ctttatctcc
    78361 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    78421 ctgttggccg tgtggttact tctgttgacg atggacgcgc tggatttgac gcacttctct
    78481 ttcctgcctg ctctgctgga agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt
    78541 ggtttgctgg attatctggc gctacgaaaa acgcatctga cgtaatctgt aaatattatt
    78601 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt
    78661 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tggcgccatt tataaattct
    78721 atttttctta cacgattcag ctaatgagtc tttattttct caccacccag ttgtcactct
    78781 aatgataatt atttgttaaa taattgtttt atttcgcatt ggttatacca attgcccgcc
    78841 aagactcctt ctgcactccc cttttgttat aacgcccttt tgcaacagct tcttaaaatc
    78901 aacctgatat gttttgcaac atatgtgacc tgacagccaa atctaagtaa caggaattta
    78961 accatgttta cagtaatgta acctccccgt aaaatgccca cacactttaa acgccaccag
    79021 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    79081 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    79141 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    79201 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc
    79261 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    79321 tggacggtgc ctatcttaat catcatcttc cttgcggtac tgacctagaa aaccactcac
    79381 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    79441 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa
    79501 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    79561 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    79621 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggg
    79681 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgaattcgac
    79741 caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc tgcgttcgaa
    79801 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    79861 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    79921 ccagaaggtg agcacagtgc acacgaaggt atggaaggca tggacatgag ccacgcggaa
    79981 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    80041 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    80101 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    80161 acctccgtcg accataaacg cctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    80221 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggt
    80281 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    80341 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    80401 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttact
    80461 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    80521 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgttgattac
    80581 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    80641 gttaccattc tgaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    80701 tgggcatcac tgtgcgcgaa cgtactgatt attgcttcct tcccaattct gacggttacc
    80761 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    80821 ggcaacatga tgatgtacat caacctgatt tgggcctggg gccacccgga agtttacatc
    80881 ctgatcctgc ctgttttcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    80941 ctgtttggtt atacctcgct ggtatgggca accgtctgca tcaccgtgct gtcgttcatc
    81001 gtttggctgc accacttctt tacgatgggt gcgagcgcga acgtaaacgc cttctttggt
    81061 atcaccacaa tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    81121 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggactatcgg ttttatcgtc
    81181 accttctcgg tgggcggtat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    81241 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    81301 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac
    81361 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    81421 ccgctgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    81481 cagttccaca ccatgctgat gattgcagcc agcggtgcag tactgattgc gctgggtatt
    81541 ctctgcctcg ttattcagat gtacgtttct attcgcgacc gcgaccagaa ctgtgacctg
    81601 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    81661 tataactttg ccgtagtgcc gcacgttcac gaacgtgatg cattctggga aatgaaagag
    81721 aaaggcgaag cgtataaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    81781 ggtgcaggta tcgtcattgc agctttctcc accatcttcg gtttcgccat gatctggcat
    81841 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    81901 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    81961 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    82021 gactgcccac gcgcacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    82081 ttggatctac ctgatgagcg actgcattct gttctcaatc ttgtttgcta cctatgccgt
    82141 tctggtgaac ggcaccgcag gcggcccgac aggtaaggac attttcgaac tgccgttcgt
    82201 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    82261 catgtacaaa aacaacaaaa gccaggttat ctcctggctg gcgttgacct ggttgtttgg
    82321 tgccggattt atcgggatga aaatctatga attccatcac ctgattgtta acggcatggg
    82381 tccggatcgc agcggcttcc tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca
    82441 cgtcacttct ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct
    82501 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    82561 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    82621 accgatcaca gcggcgcgtc ccatggcagc gtaaaaacct acatgacagg ctttatcctg
    82681 tcgatcattc tgacggtgat tccgttctgg atggtgatga caggggctgc ctctccggcc
    82741 gtaattctgg gaacaatcct ggcaatggca gtggtacagg ttctggtgca tctggtgtgc
    82801 ttcctgcaca tgaataccaa atcagatgaa ggctggaaca tgacggcgtt tgtcttcacc
    82861 gtgctaatca tcgctatcct ggttgtaggc tccatctgga ttatgtggaa cctcaactac
    82921 aacatgatga tgcactaaga gcggcggtta tgatgtttaa gcaatacctg caagtaacga
    82981 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ctggcctcaa
    83041 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    83101 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    83161 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    83221 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    83281 ggctgggggt gatgggcttt gtggtttatg tcggcgtcta tagcctgtac atgaaacgcc
    83341 actcagtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgattggct
    83401 actgtgcagt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    83461 tgtggcagat gcctcactcc tatgccatcg ccattttccg ctttaaggat taccaggcgg
    83521 caaacattcc ggtattgcca gtggtaaaag gcatttcggt ggcgaagaat cacatcacgc
    83581 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggt tacgctgggt
    83641 ataaatatct ggtggtcgcc gcggcggtta gcgtctggtg gttaggtatg gctctgcgcg
    83701 gttataaagt tgctgatgac agaatctggg cgcgcaagct gttcggcttc tctatcatcg
    83761 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtaccggac tcgcatacgc
    83821 tgctggctgc tgtgtggtaa caaaacctct ctattaaaaa ggtgctacgg cacctttttt
    83881 attgtctgag atcagagata cggctaataa ccgtatctct tgacagagtg agcagatgca
    83941 cataaattaa tcatacggtt gatagccgta ttatgtatga gatcgactcc ctacgatttc
    84001 attcagttaa tcaaagaagt cataatgcct ggaaaaacag ccgctctata cgatgtcgat
    84061 aaaacgctga aaaatgcacg ggtcgaactc aaaactagtc ccgacgccaa aaacaaactt
    84121 cgtgaagctg cgcaagccgt tggggtagat ttaagcgctt ttattttgag cgttgctatg
    84181 gaacgcgccg aaagtgtgct tgataaccaa cgtcgccgtg agctttcgaa tcaaagctgg
    84241 gaactgatga accaactcat cgctgaacct gctcaaccga cgctcgccct caaggcgtta
    84301 atgaaaagga aaaacagcga tggtcgacaa gcatgaagag attactctgc ccatagtcct
    84361 ctcctgtaat tatcagtctg atattactta tcctgggcaa aaacagtttg attgcggtaa
    84421 ccctgttatc gataaatttg tacgcgcatc gctaaagaaa agtgtgcgta atagcgactg
    84481 tgcggctaaa gcacttattg acagacaaag tggtgaactg attggcatct gtacttttac
    84541 ggcatattcg ctggaaagac aacgcgtttc tggcgtcctt cagggttcac aaccttcaga
    84601 aattggtgtt gtcagattag tcatgttggg gggagcgcgg aagtatcaaa agcggggctt
    84661 tggtcaggac ctactatgtg
//
LOCUS       DADLSE010000003        73396 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLSE010000003 DADLSE010000000
VERSION     DADLSE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.003"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..270)
                     /locus_tag="JN692_000180"
     CDS             complement(<1..270)
                     /locus_tag="JN692_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7262595.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIE"
     gene            complement(284..1961)
                     /locus_tag="JN692_000181"
                     /pseudo
     CDS             complement(284..1961)
                     /locus_tag="JN692_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(2173..2520)
                     /gene="pspE"
                     /locus_tag="JN692_000182"
     CDS             complement(2173..2520)
                     /gene="pspE"
                     /locus_tag="JN692_000182"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7262596.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIVTAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(2563..2784)
                     /gene="pspD"
                     /locus_tag="JN692_000183"
     CDS             complement(2563..2784)
                     /gene="pspD"
                     /locus_tag="JN692_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7262597.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2793..3152)
                     /gene="pspC"
                     /locus_tag="JN692_000184"
     CDS             complement(2793..3152)
                     /gene="pspC"
                     /locus_tag="JN692_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7262598.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(3152..3376)
                     /gene="pspB"
                     /locus_tag="JN692_000185"
     CDS             complement(3152..3376)
                     /gene="pspB"
                     /locus_tag="JN692_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7262599.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(3430..4098)
                     /gene="pspA"
                     /locus_tag="JN692_000186"
     CDS             complement(3430..4098)
                     /gene="pspA"
                     /locus_tag="JN692_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7262600.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDKAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            4265..5242
                     /gene="pspF"
                     /locus_tag="JN692_000187"
     CDS             4265..5242
                     /gene="pspF"
                     /locus_tag="JN692_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7262601.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(5362..6627)
                     /gene="puuE"
                     /locus_tag="JN692_000188"
     CDS             complement(5362..6627)
                     /gene="puuE"
                     /locus_tag="JN692_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7262602.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(6665..7945)
                     /locus_tag="JN692_000189"
     CDS             complement(6665..7945)
                     /locus_tag="JN692_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7262603.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7947..9434)
                     /gene="puuC"
                     /locus_tag="JN692_000190"
     CDS             complement(7947..9434)
                     /gene="puuC"
                     /locus_tag="JN692_000190"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HAY7262604.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9584..10141)
                     /gene="puuR"
                     /locus_tag="JN692_000191"
     CDS             complement(9584..10141)
                     /gene="puuR"
                     /locus_tag="JN692_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7262605.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(10168..10932)
                     /gene="puuD"
                     /locus_tag="JN692_000192"
     CDS             complement(10168..10932)
                     /gene="puuD"
                     /locus_tag="JN692_000192"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7262606.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            11144..12562
                     /locus_tag="JN692_000193"
     CDS             11144..12562
                     /locus_tag="JN692_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7262607.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGYYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRRRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12711..12875
                     /locus_tag="JN692_000194"
     CDS             12711..12875
                     /locus_tag="JN692_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262608.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12865..14250
                     /gene="puuP"
                     /locus_tag="JN692_000195"
     CDS             12865..14250
                     /gene="puuP"
                     /locus_tag="JN692_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7262609.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14384..14629
                     /locus_tag="JN692_000196"
     CDS             14384..14629
                     /locus_tag="JN692_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7262610.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDKIAEFLNQWGNE"
     gene            14942..16585
                     /gene="sapA"
                     /locus_tag="JN692_000197"
     CDS             14942..16585
                     /gene="sapA"
                     /locus_tag="JN692_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7262611.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16582..17547
                     /gene="sapB"
                     /locus_tag="JN692_000198"
     CDS             16582..17547
                     /gene="sapB"
                     /locus_tag="JN692_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7262612.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17534..18424
                     /gene="sapC"
                     /locus_tag="JN692_000199"
     CDS             17534..18424
                     /gene="sapC"
                     /locus_tag="JN692_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7262613.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18424..19416
                     /gene="sapD"
                     /locus_tag="JN692_000200"
     CDS             18424..19416
                     /gene="sapD"
                     /locus_tag="JN692_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7262614.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19418..20224
                     /gene="sapF"
                     /locus_tag="JN692_000201"
     CDS             19418..20224
                     /gene="sapF"
                     /locus_tag="JN692_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7262615.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            20292..20645
                     /locus_tag="JN692_000202"
     CDS             20292..20645
                     /locus_tag="JN692_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7262616.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            21013..21801
                     /gene="fabI"
                     /locus_tag="JN692_000203"
     CDS             21013..21801
                     /gene="fabI"
                     /locus_tag="JN692_000203"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7262617.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21945..23072
                     /locus_tag="JN692_000204"
     CDS             21945..23072
                     /locus_tag="JN692_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7262618.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            23140..25074
                     /locus_tag="JN692_000205"
     CDS             23140..25074
                     /locus_tag="JN692_000205"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7262619.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(25152..25254)
                     /locus_tag="JN692_000206"
                     /pseudo
     CDS             complement(25152..25254)
                     /locus_tag="JN692_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25308..27293
                     /gene="pdeR"
                     /locus_tag="JN692_000207"
                     /pseudo
     CDS             25308..27293
                     /gene="pdeR"
                     /locus_tag="JN692_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            27441..27614
                     /locus_tag="JN692_000208"
     CDS             27441..27614
                     /locus_tag="JN692_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262620.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            27704..28453
                     /locus_tag="JN692_000209"
     CDS             27704..28453
                     /locus_tag="JN692_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7262621.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            28722..28940
                     /gene="osmB"
                     /locus_tag="JN692_000210"
     CDS             28722..28940
                     /gene="osmB"
                     /locus_tag="JN692_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7262622.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(29066..29392)
                     /gene="yciH"
                     /locus_tag="JN692_000211"
     CDS             complement(29066..29392)
                     /gene="yciH"
                     /locus_tag="JN692_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7262623.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29392..30129)
                     /gene="pyrF"
                     /locus_tag="JN692_000212"
     CDS             complement(29392..30129)
                     /gene="pyrF"
                     /locus_tag="JN692_000212"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7262624.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(30322..31491)
                     /gene="lapB"
                     /locus_tag="JN692_000213"
     CDS             complement(30322..31491)
                     /gene="lapB"
                     /locus_tag="JN692_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7262625.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(31498..31806)
                     /gene="lapA"
                     /locus_tag="JN692_000214"
     CDS             complement(31498..31806)
                     /gene="lapA"
                     /locus_tag="JN692_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7262626.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31955..32719)
                     /gene="pgpB"
                     /locus_tag="JN692_000215"
     CDS             complement(31955..32719)
                     /gene="pgpB"
                     /locus_tag="JN692_000215"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7262627.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKIHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32889..33479
                     /gene="ribA"
                     /locus_tag="JN692_000216"
     CDS             32889..33479
                     /gene="ribA"
                     /locus_tag="JN692_000216"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7262628.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHIALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(33543..36218)
                     /gene="acnA"
                     /locus_tag="JN692_000217"
     CDS             complement(33543..36218)
                     /gene="acnA"
                     /locus_tag="JN692_000217"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7262629.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYVLAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYGEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(36382..36477)
                     /locus_tag="JN692_000218"
     CDS             complement(36382..36477)
                     /locus_tag="JN692_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262630.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(36591..36758)
                     /locus_tag="JN692_000219"
     CDS             complement(36591..36758)
                     /locus_tag="JN692_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262631.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(36761..36925)
                     /locus_tag="JN692_000220"
     CDS             complement(36761..36925)
                     /locus_tag="JN692_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7262632.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(37220..38194)
                     /gene="cysB"
                     /locus_tag="JN692_000221"
     CDS             complement(37220..38194)
                     /gene="cysB"
                     /locus_tag="JN692_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7262633.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(38404..41001)
                     /gene="topA"
                     /locus_tag="JN692_000222"
     CDS             complement(38404..41001)
                     /gene="topA"
                     /locus_tag="JN692_000222"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7262634.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            41381..41632
                     /locus_tag="JN692_000223"
     CDS             41381..41632
                     /locus_tag="JN692_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7262635.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(41668..42717)
                     /gene="sohB"
                     /locus_tag="JN692_000224"
     CDS             complement(41668..42717)
                     /gene="sohB"
                     /locus_tag="JN692_000224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7262636.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42937..43695
                     /locus_tag="JN692_000225"
     CDS             42937..43695
                     /locus_tag="JN692_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7262637.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            43692..44282
                     /gene="cobO"
                     /locus_tag="JN692_000226"
     CDS             43692..44282
                     /gene="cobO"
                     /locus_tag="JN692_000226"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7262638.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACRKVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(44311..45183)
                     /gene="rluB"
                     /locus_tag="JN692_000227"
     CDS             complement(44311..45183)
                     /gene="rluB"
                     /locus_tag="JN692_000227"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7262639.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(45284..45904)
                     /locus_tag="JN692_000228"
     CDS             complement(45284..45904)
                     /locus_tag="JN692_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7262640.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45901..46782)
                     /gene="yciV"
                     /locus_tag="JN692_000229"
     CDS             complement(45901..46782)
                     /gene="yciV"
                     /locus_tag="JN692_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7262641.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46920..46964
                     /gene="trpL"
                     /locus_tag="JN692_000230"
     CDS             46920..46964
                     /gene="trpL"
                     /locus_tag="JN692_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HAY7262642.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            47056..48618
                     /gene="trpE"
                     /locus_tag="JN692_000231"
     CDS             47056..48618
                     /gene="trpE"
                     /locus_tag="JN692_000231"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7262643.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            48618..50213
                     /gene="trpD"
                     /locus_tag="JN692_000232"
     CDS             48618..50213
                     /gene="trpD"
                     /locus_tag="JN692_000232"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7262644.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAATLVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            50217..51575
                     /gene="trpCF"
                     /locus_tag="JN692_000233"
     CDS             50217..51575
                     /gene="trpCF"
                     /locus_tag="JN692_000233"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7262645.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGL
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            51587..52780
                     /gene="trpB"
                     /locus_tag="JN692_000234"
     CDS             51587..52780
                     /gene="trpB"
                     /locus_tag="JN692_000234"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7262646.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPSGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            52780..53586
                     /gene="trpA"
                     /locus_tag="JN692_000235"
     CDS             52780..53586
                     /gene="trpA"
                     /locus_tag="JN692_000235"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7262647.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGIFAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53967..54146
                     /locus_tag="JN692_000236"
     CDS             53967..54146
                     /locus_tag="JN692_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7262648.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            54232..54732
                     /locus_tag="JN692_000237"
     CDS             54232..54732
                     /locus_tag="JN692_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7262649.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            54778..55284
                     /locus_tag="JN692_000238"
     CDS             54778..55284
                     /locus_tag="JN692_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7262650.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(55344..55982)
                     /gene="ompW"
                     /locus_tag="JN692_000239"
     CDS             complement(55344..55982)
                     /gene="ompW"
                     /locus_tag="JN692_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7262651.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            56222..56311
                     /locus_tag="JN692_000240"
                     /pseudo
     CDS             56222..56311
                     /locus_tag="JN692_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            56339..57082
                     /locus_tag="JN692_000241"
     CDS             56339..57082
                     /locus_tag="JN692_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7262652.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            57112..57651
                     /locus_tag="JN692_000242"
     CDS             57112..57651
                     /locus_tag="JN692_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7262653.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            57756..58154
                     /gene="yciA"
                     /locus_tag="JN692_000243"
     CDS             57756..58154
                     /gene="yciA"
                     /locus_tag="JN692_000243"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7262654.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(58194..58922)
                     /gene="tonB"
                     /locus_tag="JN692_000244"
     CDS             complement(58194..58922)
                     /gene="tonB"
                     /locus_tag="JN692_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7262655.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            59137..59433
                     /locus_tag="JN692_000245"
     CDS             59137..59433
                     /locus_tag="JN692_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7262656.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            59733..60986
                     /gene="kch"
                     /locus_tag="JN692_000246"
     CDS             59733..60986
                     /gene="kch"
                     /locus_tag="JN692_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7262657.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(61044..61217)
                     /locus_tag="JN692_000247"
     CDS             complement(61044..61217)
                     /locus_tag="JN692_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7262658.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            61360..62820
                     /gene="cls"
                     /locus_tag="JN692_000248"
     CDS             61360..62820
                     /gene="cls"
                     /locus_tag="JN692_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7262659.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            62855..63184
                     /locus_tag="JN692_000249"
     CDS             62855..63184
                     /locus_tag="JN692_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7262660.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(63237..64241)
                     /gene="oppF"
                     /locus_tag="JN692_000250"
     CDS             complement(63237..64241)
                     /gene="oppF"
                     /locus_tag="JN692_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7262661.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQEPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(64238..65251)
                     /gene="oppD"
                     /locus_tag="JN692_000251"
     CDS             complement(64238..65251)
                     /gene="oppD"
                     /locus_tag="JN692_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7262662.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(65263..66171)
                     /gene="oppC"
                     /locus_tag="JN692_000252"
     CDS             complement(65263..66171)
                     /gene="oppC"
                     /locus_tag="JN692_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7262663.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAISGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(66186..67106)
                     /gene="oppB"
                     /locus_tag="JN692_000253"
     CDS             complement(66186..67106)
                     /gene="oppB"
                     /locus_tag="JN692_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7262664.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(67192..68823)
                     /gene="oppA"
                     /locus_tag="JN692_000254"
     CDS             complement(67192..68823)
                     /gene="oppA"
                     /locus_tag="JN692_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7262665.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTAGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(69561..70208)
                     /gene="ychE"
                     /locus_tag="JN692_000255"
     CDS             complement(69561..70208)
                     /gene="ychE"
                     /locus_tag="JN692_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7262666.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            70685..73360
                     /gene="adhE"
                     /locus_tag="JN692_000256"
                     /gene_synonym="adhC"
     CDS             70685..73360
                     /gene="adhE"
                     /locus_tag="JN692_000256"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7262667.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAEKSA"
BASE COUNT        17937 a        18803 c        18071 g        18585 t
ORIGIN      
        1 ttcgatcact tccggcagcg agccgctacg tgaaagggtg atgactttgg tgttataagc
       61 gtcttcttcc acgggcactt gtttaacggt gatacctggg ttttcttttt caaaacgggc
      121 aatcaatttg ctgataactg cctgacgctc ctgctccacc gaagaatgca taaattcgat
      181 ggatacatgg gttttatttt cttctttaca gcctgaaatc agggcgcagg aaaccagtgc
      241 tgataacagc acgatttttg atttaatcat gagatgttcc tttttattta atccacataa
      301 cctgccaggg gcgcagcgtt atttcattac ccaatatatc aacttcacta atcagatccc
      361 gaccatgcag atcagtaata tttacatgct gaatattttt actgacatta aacaatccag
      421 tcagacaatt accatcagcg ttacttcttt gaatacgcat tacggatgaa ttaaccgtat
      481 cgatggtaaa attattatcc ggatgaaact cgttatggct gcgacgaagt gtaattaaac
      541 gcgacaactc atgatatacc gcatgcctta atgtagcttc atcgttcagt tctcgtgtta
      601 tctcttcact gtgatatttt ttacggttaa tcgcacggtt atatccgagt ttttcgacac
      661 ctgcgtaatc attacgcgag ccaagaatac tttgaatata tatcgcgggt acaccgggga
      721 aacttaacaa aatcgcatgg gcaaggataa acctggcgca acgttcttca tcgctactct
      781 cgcggcggct taacgcatcc atataggtga cattcatctc atagggactg cgcgtgccgt
      841 cgggattatt tttccagttt actaatgcac cttcctgctg taatgcctcg accagcgcta
      901 atatttcgct ttctggaagc aagccacgca gtgggttcaa cccgatgcca tcgtgagagg
      961 cgaggaagtt aaaccaggtc gttttactgg agggcaagct cagactttgc gcccactggc
     1021 acagcgcctc gacgttctgt ttttgcaccg catgtaacac cagcggcggc agcgagaact
     1081 ggtacaccat atgcgcttcg tcatcaccgt tgccaaagta agcaatgttg tctttgtgcg
     1141 gaacattggt ttcggtaatg atcactgtac ctggtgctac gtcatcaata atcgaccgta
     1201 acagcttgat aatcagatgt gttttttcca gatggatgca gcttgttccc ggctctttcc
     1261 acataaagcc aacggcatcc aggcggacat actcagcacc tttctcaagg taacagagta
     1321 aaacatccac catcgccagc aacacttcag ggctacggta attcaggtca atttgagtca
     1381 ctaaaggtgg tccataaatg gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc
     1441 ggtaacgcac gcggacgggt gacggcactg agatcggttt tcggatcaac cgcaataaag
     1501 aaatcttcaa aacccggaag ctgttgtaaa tagtttttaa accattcact ttttgcagac
     1561 atatggttgc agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc
     1621 tgccactccc ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa tccatcatca
     1681 gatgaccatg ggtaaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga
     1741 tggtagaact gattaaatat gggtagtggt tttaaatcat tactgtgaaa ttgatcggca
     1801 taggtaatta acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat
     1861 cgtttcgcac tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca
     1921 ccgtagattt cgtccaggta atccgtaatt ttctgtttca tgttgccttt tcgtggtagc
     1981 ggtcccatga agcgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt
     2041 ttagtcacgc aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa
     2101 taatctatag ggatgtaaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca
     2161 tggtgaattc ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt
     2221 ctcaacgtgg gtatatccca tctcgctaag gatctctttt gcctgcccgg actggcgtcc
     2281 ggcattgcaa tacactttca cggtgtcgtt tttatccgga acggcggtga caatgcgctc
     2341 tttcacttcc ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc
     2401 tggaacacga acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc
     2461 cagagcaagt aagccttttt ttaaacatgt aagcgtcctt ctatggatga aaattgccat
     2521 ccattatacg cctgagattt aacaattttt gcgaaactcc cctcaccttt tataacgctg
     2581 tgccagttta ttagcagccc gacttaacag cggttccagc gccacagcca gcaacatttt
     2641 cagcggtcgg cgagcaactg attttatcgc ccagcccgcc acacccgccg ggccatagcg
     2701 cagtgcggta agaagtacca gcttgcctgc taatttgaaa ccaggcttca ccttttgccc
     2761 ggcctgttgc cagcgagtat tcataacttt cctcacagtt gacggaaacg gctacgtaac
     2821 gtgaaagtat cggaagtgac ataacgttcc atctcgcgta aacgcgtttc acttgccgcc
     2881 agttcacggt cgacttcatc cagcaattcg ctgctggaag gtagctgctc accaaaggcc
     2941 atgttgtccg gcattggatc aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac
     3001 agcgccagac cgaagaaaat cgacagcacc accaggatac gcaccagttt taccggtaca
     3061 tcaaaatagt tggcaatccc ggcgcagacg ccgcggacca tgccctgctg tggaatacgc
     3121 cataattttt tattgagatt aatgcccgcc attagcgatc cctccagttc ggatgttctg
     3181 catcaagaat agattccagc gcctgaatac gttcgcgcat ccgttttgct tcatcagcca
     3241 gttgcgctaa tcgctgctgc tcactttgcg acaattcact gcgaccagaa cgattgctgt
     3301 aatgcagcca taaccagatc ggtaaaacaa acagcacaaa aatggttaac ggaatagcca
     3361 gaaatagcgc gctcataagt actccttaga cgatgagcgg cggcgcgttc ggacgccgcc
     3421 tggatgttat tattgattgt cttgcttcat tttggctttt aattgtgcca gttgttcgct
     3481 gattgcatca tcggctttca gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa
     3541 gctgtggctt tctgcttccg cttccatctg gtcaatacga cgttcgaaag attcgaaacg
     3601 agccattgct ttatccagtt tgccactgtc cagctgacga cgcacatcgc gcgacgagtt
     3661 tgccgcctga tgacgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt
     3721 ttccagctcg ccaatctctt tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc
     3781 atgttccagg gacttaatca gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc
     3841 cagatcctct ctctctttca gcagcgccag ttcggctttt tcctgccatt caacttcacg
     3901 cgccgacgct tgttcaatac ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga
     3961 agtagaacgt acttcaacca gtgtatcttc catctcctgg atcatcagac gcaccagttt
     4021 ctgtggatct tccgctttct ctaacagagc gttgatgttg gcattcacga tgtcggcaaa
     4081 gcgagaaaaa atacccataa tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg
     4141 aactgttaat acaatttgcg tgccaatttt ttatcttttt gatttataaa gatctgattg
     4201 aagaatcaac agcaacatgc caggatgaat tagcgaatta cactaacaag tggcgaattt
     4261 catcatggcc gaatacaaag ataatttact tggtgaggcg aacagctttc tcgaagtgct
     4321 ggaacaggtt tcgcatctcg caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg
     4381 caccggtaaa gagttgattg ccagccgcct gcattatctc tcctcccgtt ggcaagggcc
     4441 gtttatttcc cttaactgcg cggcgttaaa tgaaaatctg ctggattccg aactgtttgg
     4501 tcacgaagcg ggggcgttta ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc
     4561 cgacggcggt acgctatttc ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa
     4621 attattgcgt gtgattgagt acggtgaact ggagcgcgtt ggcggtagcc agccgttgca
     4681 ggtgaatgtg cggttggtat gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg
     4741 tacttttcgc gctgacctgc tcgaccggct ggctttcgat gtagtgcaac tgccgcccct
     4801 gcgcgagcgc gaaagcgaca ttatgctgat ggcagaacac tttgccattc agatgtgtcg
     4861 ggaaatcaag ctgcctctgt tcccggggtt tacggagcgc gccagagaaa cattgctgaa
     4921 ttatcgttgg ccgggaaata ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg
     4981 ccacggcacc agcgattatc cgcttgatga catcattatt gatcccttta aacggcgtcc
     5041 acctgaagaa gccatcgccg tttcagaaaa tacctcgctt ccgacgctgc cgctggattt
     5101 acgcgagttt cagatgcagc aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa
     5161 atataaccag aagcgcgcgg cagaattact gggtttaact tatcatcagt tccgcgcgtt
     5221 gttgaaaaag caccagattt agcgaagttg agcaatacaa cttgccggat gcggcgtgaa
     5281 tgctttattc ggcatacata atcaatacgt cgtttgcagg cctgataagc gcagcgcatc
     5341 agacattatt gcgttgggct attaatcgct cagcgcatcc tgcaaaattt tcattgccgc
     5401 atcgaattgc gcatccggga tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc
     5461 gccacaggtc agcaggagca gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat
     5521 cgccgctgac ggctcgcccg tttgcggatc gttaaactct gccgcaatca tcgaccccag
     5581 gccgcgtacc gcagcaatgg ccggaacgct ttctttggca tcaatcaacg tgtttttgag
     5641 acgctggccc agttgattcg cgcgttcgca gagtgattct ttgtcgataa tattgagcac
     5701 cgcgtgcgcg gcagccaccg ccagcgggtt cccggcgtag gtgccaccca acccgcccgg
     5761 cgcgggcgcg tccataatat tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc
     5821 gaggcttttc gccatcgtca ttaagtccgg cttatcggcg taatgatcca tggcaaacag
     5881 tttaccggta cgcgcaaagc cgctttgcac ttcatcggca atcatcacaa taccgtgctc
     5941 gtcgcacagg cggcgaatgg cggcaaccag ctcttttggc gcaacgttga aaccgccctc
     6001 gccctgcacc ggttcgaaaa taatggccgc gacctgcttc gcttcgatgt ctgatttaaa
     6061 caagcgttcg atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata
     6121 aggtacgtga tacaccgaac cagggaacgg gccgaagccg attttgtacg gcgcaacttt
     6181 tccggtcagc gccatggtca tatatgtacg accgtggaag ccaccgctaa acgcaatcac
     6241 gccaggtcgt ccggtatggg cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc
     6301 ggtagtgaag aatgcggttt tagcctgccc gctcactggg gcaagggcgt taattttctc
     6361 cgccagcgtg acgtagcttt cgtacggcac aatctgatac gccgtgtggg taaactgttg
     6421 caattgctgc tccaccgccg cgaccagatc agggtggcga tgcccggtat tcagcaccgc
     6481 aatgcctgcg gcgaaatcga tgtattcgtt gccctctacg tccttcagcg tagcgttttc
     6541 tgccgactgg gcgaagaagt tacacatcac gccaaccccg cgtggggtag cagaaagacg
     6601 acgctgatgg aattcattgt tgctcatgtt tcacaccttt tgactcttca ctgttaatcg
     6661 gaaattaaac gcccagacga tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg
     6721 gcacgcgcag cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg aaggcatcga
     6781 aacgctcggc gtcaccgcgc agcagttcag caatcaaacg cccggcaaga tgggtacagg
     6841 tgacgccgtg gccgctgtaa ccctgcatgt aatagatgtt ggtatcaagg cggccgaact
     6901 gcggcatgcg cgacaaggtc agcaggaagt tgcccgtcca gcggtaatca attttcacgc
     6961 ccttcaactg agggaaggtt tttagtagtt tcggtaccac aagacgctca acgtcatccg
     7021 ggttgcgtgc accatacact acacccccgc cgtacagcag gcggttgtcg gcggtaagac
     7081 ggtagtaatc aagcagatag ttacagtctt ccacacagta gtttttcggg atcagcgaac
     7141 gggctaaatc ttccgacagc cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc
     7201 gtttcgctag ttccggctct actttatcgc ccagatacgc attcccggcg acaatcacat
     7261 acttcgccgt cacctgacca ttggcagtcc gaaccacggc tggcgtggtg tgctggatct
     7321 gcgtcacggc ggaaagctca tacacgcgcc cgccgttcag gcggatggca tcggcttcac
     7381 caatggcgag gtttagcggg tgaatatgcc caccgctgtg atccagcagc gcaccagtgt
     7441 agcgatcgct ggcgacctcg cggcgaatag cgttggcgtc cagcagctcc agctgtttat
     7501 tgccgtagcg ttcccagttc tctttctgct cttcgagtgt ggcgagttgt ttatcattca
     7561 tcgccacaaa cagaccgccg gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt
     7621 cgcggatgat ctcgccaccc tcgaacatca tgctgccgag catacgggcg gtgtccatgc
     7681 cgtagctttt ttcgatcacg tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat
     7741 tgcgcccgct tgcgccaaag ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg
     7801 cttccgccag atgcagcgcg gaggagagcc cggtatagcc gccgccaacc acgcaaacgt
     7861 cgcaggtgat tgactcattc agtgtgtcga atggtgcata tttattcgca ctggcggcgt
     7921 agtaactgct ggtatgttcg gtcatttcag gcctccaggc ttatccagat ggttttcagt
     7981 tcagtgaatt tttcaagggc atgcagggat ttgtcgcgac cgttgccgct ctgcttatag
     8041 ccgccaaacg gcacggtcat atcgccgtcg ttgtagttat tgacgaagac ggaaccggct
     8101 ttcaggcgtc ggctcatgcg gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca
     8161 aggccgtact ggctgtcgtt ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg
     8221 accaccagca ccggaccgaa aatctcttcg cggcttaagg acgcattcgg gtccacatcc
     8281 acgaagatgg tcgggccgat ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt
     8341 tgccccttgc cttcgccttc acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg
     8401 attaaggtgc ccatggtggt tgcgggatca agtggatggc ccggctgcca gttttgcgcc
     8461 tgctgtttta acagggctaa gaattcatcg gcgatgctct cttccagtaa caaacgcgtt
     8521 ccggcgatgc acacctggcc ctggttgtag aagatgcctg cggcggtggc gcttgccgct
     8581 ttttgcagat ccggacaatc ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc
     8641 cagacacgtt tcatgttgct atcacccgcg tctttcagca gttgtttacc cgtacgcgtg
     8701 gagccggtaa aggcgatggc atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca
     8761 tgaccaaaac ccgtcaccac gttcaacaca ccatccggca agcctgcttc tttcgccagc
     8821 cccgcgagac gaatcgcact gagcggtgat ttttcagacg gttttagaat cacgctgttt
     8881 cccgccgcca gcgccgggcc gagtttccag caagtcagca acagcgggaa gttccacggc
     8941 acgatggcgg caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg
     9001 gtggtcgcca cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc
     9061 gccgcgccgg gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt
     9121 tccagcagtg ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc
     9181 gctttacgtt tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct
     9241 gcgctcaccg cacggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc
     9301 tgggtgaccg gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg
     9361 ttaataaata agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga
     9421 tgatgaaaat tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg
     9481 tcagggcgca gagatccccg ggctgcatgc ccgtttattt ctggcgacat tctttatgga
     9541 ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg
     9601 tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc
     9661 cggtattaat ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca
     9721 gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc
     9781 cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac
     9841 cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat
     9901 taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca
     9961 gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt
    10021 ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa
    10081 gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca
    10141 tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg
    10201 ccaagcggtg ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca
    10261 ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac
    10321 taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg
    10381 tccatgtaga gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc
    10441 cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg
    10501 atcttcccga tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc
    10561 ggttgccacc accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc
    10621 gagtgcggca ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc
    10681 atcgccgttt tcaccatata ggtgcggctg cacattgctg ggactactag gaagataaat
    10741 gccatcgagt ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag
    10801 cgcaattggc aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg
    10861 ggtcgcatga cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat
    10921 tatattttcc atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa
    10981 atgaccttta tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca
    11041 atttaacatt tgacaaacat ttagtttgca tacagattcg aatgatggtc attatatttt
    11101 acgctttgat aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt
    11161 tgaagtagag aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga
    11221 agtgaaacgc tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga
    11281 tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa
    11341 agggtattat ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga
    11401 agcaggtctg ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc
    11461 cttaactcct tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga
    11521 tgaagatggc gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca
    11581 gctgcgccag cgcggattgt tcccggttgt agcggtagag ctggagttct atttactgga
    11641 tcgccggcgc gacgctgaag gggatctgca accgccctgc gcgcctggca ccgatgaccg
    11701 caatacacaa agccaggttt actccgttga taacctcaac cacttcgccg acgtgctcaa
    11761 tgatattgat gaactggcgc agttgcagct gattccggca gatggcgcgg tcgctgaggc
    11821 ctcgccgggt cagtttgaaa tcaaccttta ccatactgat aacgtgctgg aagcctgcga
    11881 tgatgcgctg gcactaaaac gcctggtgcg tctgatggca gaaaagcata agatgcacgc
    11941 cacttttatg gcgaagccgt atgaagagca cgcgggcagc gggatgcata tccatatcag
    12001 tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca gaaggcgaag attcgccgct
    12061 gctgaaaaag atgctcgccg ggatgattga cctgatgccg tcgtcgatgg cgttgctggc
    12121 accaaacgtg aactcgtatc gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc
    12181 gtggggccat aacaaccgca ccgtcgccct gcgtattccg tgcggcgacc gtcataatca
    12241 ccgcgtggaa tatcacgtgg cgggtgccga tgccaaccca tatctggtga tggcagcgat
    12301 ttttgccggt attttgcatg gccttgataa cgagctgccg ttgcaggaag aagtcgaagg
    12361 caacgggctg gaacaggaag gcttaccctt cccgattcgc cagagcgatg ccctgggtga
    12421 gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca
    12481 cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc atcacagaaa cagaaattga
    12541 gtggatgttg aaaaacgcgt aattcccctt gcggggttgt ttcatcggcc tgagcggagc
    12601 caggcctgac atttgctggt aatccagaca tttcccggat gtcgcgttat ggagcgcagg
    12661 cggcaacggg cttccgttaa cgattaagac tgtgacgagg aaatgagatg atgccgatga
    12721 ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc tcctgctact gactggactc
    12781 cgcccccttt gcttcccttg ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct
    12841 tattacgcag gggaggcagc aattatggct attaattcac cactgaatat tgctgcgcaa
    12901 cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtaat gatgggtctg
    12961 gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg cattagcgac
    13021 ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg tgctgtttac cgctatcagc
    13081 tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta cgcgcaaaag
    13141 tcgattaacc cgcacgtcgg atttatggtc ggctggtcat cactgctgga ttatctcttt
    13201 ttgccaatga tcaacgtttt gttggcgaaa atctatctct ccgccctctt cccggaagtg
    13261 ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc
    13321 gtcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc catcatggtg
    13381 gtgtttatct tcctggtggt tcagggactg cataaaggag aaggcgttgg caccgtctgg
    13441 tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac cggtgcgacg
    13501 attgtctgtt tctcgttcct cggtttcgat gcggtgacca cgctttcgga agagacgccg
    13561 gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtatatgg cggcgttatc
    13621 tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg atatcagccg ctttaaagac
    13681 ccggacgccg cactgcctga aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt
    13741 ttcctctgca ccacgtttgt gaacacatta gcgtctggcc tggcttcaca tgccagcgtg
    13801 tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat
    13861 gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat tgtcgcgctg
    13921 tcggcgctgt tctttgattt agtcaccgcg acagcactga ttaacttcgg tgctctggtg
    13981 gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat
    14041 aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc gctgacggtg
    14101 ggtgtgctgt gggttaacct agagtcaacg tcactgacac tcggtctggt atgggcttcg
    14161 ttaggcggcg catatttgtg gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa
    14221 ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct
    14281 gagggtaacg cgccgcgcca ttaactataa aaagatgtat gacccggatc acgagtgcta
    14341 tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc acgatatccc
    14401 gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga
    14461 ggctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt tgatgaacaa
    14521 tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga ttaatcccgt
    14581 acgcattgat aagatcgcag agtttttgaa tcaatggggt aatgagtaat tatttaattg
    14641 cagacgatcg gtcagtatcg ttgtccggat aaagcgttca cgccgcatcc ggctaacaat
    14701 gacacgcact gcagcgtgct ttaatgggtt tctcacgatc gtaccgctct gctcctcgct
    14761 aagttgttct gtgagttata gttatagaga aatcacgggt acgaatgttg aacagatggc
    14821 gacgccacct gttcaacgac caaccacacg gccctcatta cgacccctga acacaaacgg
    14881 taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat cccttaatat
    14941 tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg gtcaggcaat
    15001 cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg tctattgcgt
    15061 cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa ttgtcgatac
    15121 ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct atcgcctgat
    15181 gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc gcttccacct
    15241 gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta aaatgaatgc
    15301 cgacgatgtg gtgtttactt tccagcgaat ttttgaccgc aacaacccgt ggcataacgt
    15361 caacggcagc aacttcccct acttcgacag cctgcaattt gccgataacg tgaaaagcgt
    15421 ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg cttctttttt
    15481 gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc ggaagttaga
    15541 gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt
    15601 gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact tctggcgcgg
    15661 taaaccgtta atgccgcagg tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc
    15721 gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc agctatccat
    15781 tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct
    15841 ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg cactggcact
    15901 ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg aaacggcggc
    15961 ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta ctgaatacaa
    16021 tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg gaaaacttaa cgctgaaact
    16081 gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat
    16141 tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt
    16201 tcaggaggcg cggttgatgg atatgagcca tgatctgacg ttatccggtt gggcgacgga
    16261 cagtaacgac ccggacagtt tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca
    16321 gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta aggcgctctc
    16381 ctcgcagcag ctggcagcgc gtattgaagc ctatgacgaa gcgcagagta ttctggcgca
    16441 ggaattacct attctgccgc tggcgtcatc attgcgtttg caggcctatc ggtacgatat
    16501 caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa
    16561 acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt gttattgatt
    16621 gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac tccacacgcg
    16681 ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaatgg cctgatccac
    16741 tgggattttg gtgtttccag cattaacggt caaccaattg ccgaacagtt aaaagaggtc
    16801 ttcccggcga cgatagaact gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc
    16861 cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt gatcaacgcc
    16921 atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt gacactgttt
    16981 tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg
    17041 aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc atggcgggat
    17101 gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct gtcggttgcg
    17161 ccaacaactg aagtgatccg cctgatgcgt atcagcacta ttgaagtgta tgaccagaac
    17221 tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt
    17281 ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc caccatgctg
    17341 acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg ctggttaatt
    17401 aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt gtgtggctca
    17461 ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa ccctctgaaa
    17521 cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc cgggcacgct
    17581 gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc tgtacggctg
    17641 cgcgggactg gctgtactgt gtatttttgg cggctggttt gcgccttacg gcatcgacca
    17701 gcaatttctc ggttatcaat tattgccgcc atcatggtcg cgctatggcg aagtttcttt
    17761 cttcctgggg actgacgacc tcggacgtga tgttttaagc cgtttactga gcggagctgc
    17821 gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg gcctggtact
    17881 gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc atattctgga
    17941 taccttactg gcgatccctt cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc
    18001 gagtttgtcc cacgctatgt ttgctgtctg gctggcattg ctgccgcgta tggtacgttc
    18061 gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg cccgtctgga
    18121 tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca ccgccgggct
    18181 ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg cgctgggctt
    18241 tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct
    18301 ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa ttatgattag
    18361 cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga
    18421 ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg
    18481 gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt
    18541 ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt gaataaagat
    18601 aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc
    18661 gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca ggaaccgcag
    18721 tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat cccagcctgg
    18781 acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc cattgaactg
    18841 ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc ctatgagttg
    18901 accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca accgcgtctg
    18961 ctgattgctg acgaaccgac aaactcaatg gagccaacaa cccaggcgca aatctttcgc
    19021 ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag ccatgactta
    19081 caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca aacagtggaa
    19141 accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca ggcgctgatc
    19201 cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc
    19261 ggcgctatcc cattgctgga acagttaccg attggttgtc gtctggggcc acgttgcccg
    19321 tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat
    19381 gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc tgcttgaagt
    19441 gcgtaattta agtaaaacgt tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga
    19501 agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga ttattggcga
    19561 gaatggttcg ggtaaatcca cactggcaaa aatgctggcg ggaatgatag aacctaccag
    19621 cggcgagtta ttgattgacg atcatccgct gcattttggc gattattcct tccgtagtca
    19681 gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc aacgtatctc
    19741 gcaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa
    19801 acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca gttactatcc
    19861 gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg
    19921 cccaaaagtc attattgccg atgaagccct cgcttcactg gatatgtcga tgcgttcgca
    19981 gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata tttatgttac
    20041 ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc atcagggcga
    20101 ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa
    20161 acggctgatt gccgggcact ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg
    20221 ctaattttct gaatcctcct tccagattat tcacctcatt ctgacaagag attaaatctt
    20281 gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga cgcaatctca
    20341 ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt gatttcaaat
    20401 ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg
    20461 tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat accaggcgca
    20521 ctagctggct tgagtcgcag ggctggaccg tgctgcgttt ctggaataac gagattgatt
    20581 gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccgg tcaccctcac
    20641 cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa catggagaca
    20701 gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa aagggttagg
    20761 ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg tcccctcgcc
    20821 cctctgggga gagggttagg gtaaggggaa aagcgccccc cctgccgcag cctgctccgg
    20881 tcggacctgg caactatagc tactcacagc caggttgatt ataataaccg tttatctgtt
    20941 cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc atcaacaata
    21001 aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg tgttgccagc
    21061 aaactgtcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc tgaactggca
    21121 ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc tcaattgggt
    21181 tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac catgttcgct
    21241 gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg ttttgcacct
    21301 ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt caaaattgcc
    21361 cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc catgctgaat
    21421 ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat cccgaactac
    21481 aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg
    21541 atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg tactctggcg
    21601 gcctccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt
    21661 cgccgtaccg ttactattga agatgtgggt aactctgcgg cattcctgtg ctccgatctc
    21721 tctgccggta tctccggtga agtagtccac gttgacggcg gtttcagcat cgctgcaatg
    21781 aacgaactcg aactgaaata atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg
    21841 ttatctctgt tatacctttc tgatatttgt tatcgccgat ccgtctttct ccccttcccg
    21901 ccttgcgtca ggataacgat ttcctttacg accaaggagc gcccatggaa caacgccaca
    21961 tcaccggcaa aagccactgg tatcatgaaa cgcaatccag tactacggag tatgacgttc
    22021 tgcctctggt cccggaagcc gcaaaggtca gcgatccctt tctgctcgac gtgatccttg
    22081 atgaagagac gctggccccc ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg
    22141 agttgttccc tgaccagctt accgtgaccc gttcacaaac cttcaccgct tatgaacgct
    22201 tgtcgacggc cctgacggtt gctcaggttt gcggcgtcca gcggttatgt aactactatt
    22261 cggcgcgact tacgccgctc cccggacctg attcctccag ggaaagtaat catcggttgg
    22321 cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc ttctattatc gacaaccgat
    22381 cgcgccagca tctgaatgac gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa
    22441 tcattggttt tattggcttt caggcgcgga caattgcgac atttcaggct tatctcgggc
    22501 atccggtacg ctggttaccc gggctggcga tacaaaacta cgccgacgcg tcactgtttg
    22561 ctgatgaatc aatacgctgg cgaagcagct atgaggtgga aaaactacct gaagagcaca
    22621 caaaaagttc aactgcagaa ctttgccaac tggccgaaac actctctctc caccctattt
    22681 cactttccct tctcgaaaag ttgttaaaca gcacacgggt caatacacag ccggataatc
    22741 agcttgcggc gttgttatgc gcgcgtataa atggcagtcc tgcttgtttt tccacctgta
    22801 tggattcatc aaatgaatat aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa
    22861 ttaaccgatg ggctgaccgt cattctgttg agcgcgctac cgttcaggcg atacaatggc
    22921 tgacccgagc acccgatcgc tttagcgccg cccagttcag ccctttactc gaacacgaaa
    22981 aatcatcaac gcagattatt aatctgctgg tatggagcgg gctgtgtggc tggataaatc
    23041 gcttaaaaat cgcgttgggt gagacatatt aaccttgccg cgtcagacag attcgcgtaa
    23101 aactgtcagc cgctctaatg gccaccaaaa tagacaatta tgtttcagga caacccgctg
    23161 ctagcgcagc ttaaacagca actgcattcc cagacgccac gcgctgaagg ggtggtaaaa
    23221 gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg
    23281 ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta tcgcggtgat ccacagtgaa
    23341 aaagaacgtg aatccgcaga gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg
    23401 ggtaaagttc agggcaaaaa tgaccgtctg accatcgttc ctgatcatcc acttttaaaa
    23461 gacgccattc cttgccgcgc agcccgtggc ctgaaccacg agtttaaaga aggcgactgg
    23521 gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc gttctttcta tgctgaactg
    23581 acacaataca tcacttttgg tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc
    23641 cataatctgg aaaaagaagc accagacggc gtcgctaccg aaatgctcga tgaaggtctg
    23701 gttcgtgaag atctgacctc gctggatttt gtcaccatcg acagtgccag cactgaagat
    23761 atggatgacg cccttttcgc taaggcgctg ccggatgaca aacttcagct gattgtggcg
    23821 attgccgatc caaccgcgtg gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt
    23881 cgcgcattca ccaactatct gcctggcttc aacatcccta tgctgcctcg cgagctttct
    23941 gacgatctct gctcactgcg cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg
    24001 ctctccgctg atggcaccat tgaagataat atcgaattct ttgccgccac catcgaatcc
    24061 aaagcgaagc tggtgtatga ccaggtttct gactggctgg aaaataccgg tgactggaag
    24121 cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc
    24181 ggcgagtggc gtcataacca cgcactggtg tttaaagatc gcccggatta ccgctttatt
    24241 ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt
    24301 atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg cccgcgtact gcgcgataag
    24361 ctcggttttg gcatctataa cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg
    24421 gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg aagaagtgct cacgctggac
    24481 ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa ctggtttcct cgacagccgc
    24541 attcgtcgct tccagtcatt tgctgaaatt agcactgaac ccggtcctca ctttggcctc
    24601 ggtctggaag catacgccac ctggacttcg ccgatccgta aatatggcga catgatcaac
    24661 caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc
    24721 actgtccaaa tggccgaacg tcgccgtctc aaccggatgg cagaacgtga tgttggcgac
    24781 tggttatacg cacgcttcct gaaagataaa gccgggaccg gcacccgttt cgcggcggaa
    24841 attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc
    24901 tttattccgg cacctttctt acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac
    24961 ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc
    25021 attgccgaag tccgcatgga aacccgcagc attattgcgc gcccggtcgc gtaatctcct
    25081 ttcacggccc attcctcatg gatgggccgt ttatttcccc gctctacctt cgttatatcc
    25141 aggcagattt tttaactgac tttcgtttga aaactggcgt ttttccaaaa ctggattaca
    25201 aataaatata taaaaaacaa tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt
    25261 ccttttattt aaccgcggac tccgctgtta accggaggat atgcatcatg aaaaccgtta
    25321 gggagtccac aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg
    25381 aaagcagcga tgctttgcgc ttttctacca ccgaaaccac agaacctgat cgtacattgc
    25441 agttatctgc cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga
    25501 tgatgagtct gaccgtcgat gaaagcgatc tttctgtgca tctggtagga cgaaaaatca
    25561 ataaacggga atgggctggc aacgcgtctg catggcatga cacaccggcg gttgctcgtg
    25621 atttatcaca cgggctttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag
    25681 tgattctcga cagccggggg aatatccaac gcttcaatcg gttatgtgaa gattacacag
    25741 ggttgaaaga acacgacgtc attgggcaaa gcgtgtttaa actgtttatg agccgtcgtg
    25801 aagctgcggc atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag
    25861 tcgaactgtg gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg
    25921 tccacagcgg cagtggcaaa aacgagattt ttttaatctg ttccggtacc gacattaccg
    25981 aagagcgccg cactcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac
    26041 tgccgaatcg taacgcaatg caggagttaa tcgatcacgc tattaatcag gcagataaca
    26101 ataaagttgg ggttgtgtat cttgatttgg ataattttaa aaaggtcaac gacgcctatg
    26161 ggcatttgtt tggtgaccag ttattacgcg acgtgtcatt ggctatttta agctgtctcg
    26221 aacatgacca ggtgttggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaata
    26281 cctcacaaag cgcgctggaa gcaatggcat cacgaatttt gacccgctta cgcctcccct
    26341 ttcgtattgg tttaattgaa gtttatacca gctgttcagt aggtatttcg ctctctcccg
    26401 aacatggttc ggacagcgcg actattattc gtcacgccga cacagcaatg tacacagcga
    26461 aggaaggcgg acgaggacaa ttttgcgttt ttactccaga aatgaatcag cgggtatttg
    26521 aatatctctg gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc
    26581 actatcaacc gaaaatcacc tagcgtggcg aagtgcgcag tctggaagca ctagtacgtt
    26641 ggcagtcacc tgaacgtggg ttgattccac cgttggactt catttcctac gccgaagagt
    26701 cagggctaat tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtggcaa
    26761 agtggcggga taaaggcata aacctgcgag tggcggtaaa tatttctgca cgtcagctcg
    26821 ccgatcaaac cattttcacc gccctgaaac aggttctcca ggaactcaat tttgaatact
    26881 gccctataga tgttgaactg acagagagtt gtctgattga gaatgatgaa ctggcactgt
    26941 ctgttattca acaatttagc caactaggtg cgcaagtgca tctggatgat tttggtaccg
    27001 gctactcttc actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg
    27061 tttttgttcg agatattcac aaacaacctg tctcgcagtc actggtccgg gcgatcgtcg
    27121 ctgtggccca ggcattgaat cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag
    27181 atgctttttt aaccaagaac gggatcaatg agcggcaagg atttttgttt gccaaaccga
    27241 tgcccgccgt cgccttcgaa cgctggtata aatgctatct gaagcgcgca taagtagatg
    27301 aatatatata cttagcgcta tatcccgtca ttatatatcg tattcctgga tgataattag
    27361 ccgttgctgt taaacacttc tcttttagcg gcaatttttg cattattcat tttccccctt
    27421 ttgctataag gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg
    27481 atattgtgtt agatatcctt gtggcaggcg attaccactc tgcgatccat aatctcgaga
    27541 tacttaaagc agaactcttg cgccaggttg ctgaatctac gccagacatc ccgaagacac
    27601 cgtgggagat ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca
    27661 gtattttttt atttagtatt ataacgttat aagaattaca gcgatgaatt cccgacaaca
    27721 aactattcta cagatggtca ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc
    27781 cactggagtt tctgaagtta ccattcgcca ggatctcaac accctcgaaa aactgagtta
    27841 cctccgccgt gcacatggct ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat
    27901 gatgagcaac tatacgctga agcgtgaact cgccgagttt gccgcgtcac tggttcaacc
    27961 gggcgaaacc atctttatcg aaaatggcag cagcaatgcc ctgctcgccc ggactctggg
    28021 ggagcagaag aaaaatgtca ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa
    28081 agacgcgcct tgtgaagtca ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat
    28141 ggttggccct ttgacacgcc agtgcatcca gcaggtacat ttcagcaaag catttattgg
    28201 tattgatggc tggcaacctg aaactggatt caccggtcgc gatatgatgc gcaccgatgt
    28261 ggtcaatgcc gtgctggaaa aagagtgcga agcgatagtc ctgactgaca gctcgaaatt
    28321 tggtgctgta cattcatact ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga
    28381 ttcgaaaata cgcgccagcg atctgatgca tcttgagcac agtaaactca ccgttcacgt
    28441 cgttgacatt taaccatttc ccttctctac ggatgatttg cagtttggca aatcatccgc
    28501 tctaagatga ttcctggttg ataattaaga ctatttacct gttattaaca ctctcaagat
    28561 ataaaattat tatcagcgat ataacaggaa gtaattatca cctgcgtgat ataaccctgc
    28621 gcgcgagcag atttcacgga ataatttcac caggcttatt cttagctatt atagttatag
    28681 agagcttact tccgtgaatc ataaattcag gagagagtat tatgtttgta acgagcaaaa
    28741 aaatgaccgc ggctgttctg gcaattactt tggcaatgtc tctgagtgcc tgttctaact
    28801 ggtctaaacg ggaccgcaac accgcaatcg gcgcgggtgc aggggcatta ggcggtgcag
    28861 tactgaccga tggcagtacg ttgggtacat taggtggtgc agctgtcggt ggtgttattg
    28921 gtcaccaggt cggtaaataa tcgtaaataa atgtcgatta tcgactcgca attataatac
    28981 gcttgattct gttcagtacg aaataactga ataatacaca cagtaaaata ttcgaaagcc
    29041 acggatatat ccgtggcttt ttatgttaac cgcctgcgag ttttaccttc atccctttcg
    29101 cttcaagtag tgattttaat aaatcacgct tatcgccctg gatttcaata actccatctt
    29161 taactgctcc gccgcagccg cattttttct tcagttccgc tgcaagtttt gtcagttcgg
    29221 catcatcgag atcgataccg gtaatcaggc aaacgccctt acctttacgt ccactggtct
    29281 gacgctgaat acgcaccgct ccatcgcctt tagggcgtac tggggccgct ttgggctcat
    29341 caatacgccc ggtttccgtt gagtagacca gacggctgtt ggaatcactc atcatgcact
    29401 ccgctgtaaa gaggcgttga tcgctttcag tgtctgcgct ggatctaccg attgcgttac
    29461 cgggcgacca atcaccatat aatcaacacc agccgccaac gcctgttctg gcgtcataat
    29521 gcggcgctgg tcaccagctt cactcccctg cggacgaatg cccggtgtaa ccagtttgaa
    29581 ctcctgaccg aatacctgtt taaagcgcac agcttcctga gcagaacaca ccacaccatc
    29641 aaggccacat ttttgcgtca gtgctgccag acgttctgca taatctgcag gtgacagtgt
    29701 cacgccaaga tcggccaggt cgctggcttc catgctggtc aacactgtca cagcaatcaa
    29761 aagcggtgca tctttgccaa acggaaccag tgcctcacgc gctgcggtca tcatacgcgc
    29821 cccaccggag gcatgaacat tcaccatcca cacgcctaag tcagctgcag cagcgacagc
    29881 gtgcgctgca gtgttgggga tatcgtggaa tttcaggtca agaaagatat caaaaccacg
    29941 ctgttgaagt tcgcgcacaa actgtggccc aaacaatgta aacatctctt taccgacctt
    30001 cagacgacaa tcgcgtgggt cgatcttgtc gacaaaggac agcgcgtcat cacaattatg
    30061 ataatcaagg gcaacaacca caggagaatt cgtaacagcg cgggaagaag atgaagcagt
    30121 taacgtcatg accagacctt cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac
    30181 ggcgagcatt ctacccgtgg aaataaaaaa ttaacagttg cgatttttcc cctagcaggc
    30241 tttttctgct ccgtcgttgc cggagcacaa tgggacatca ataactagta tgttgtaact
    30301 aaagtcggat ttttttaaaa attacaggcc atcaagaccg cgaatcggtt taatagttga
    30361 ccaggcacga caagacggac aatgccagta gagggtgtat gcggtaaaac cacatttctg
    30421 gcagctataa cgaggcttac tacgcacctt ctcgccaacc atgtcacgca gcaccatcag
    30481 actctctttg gcgcgccctt cttccgcttc atttaagtgg taatccatta gcttatggaa
    30541 tacacgcatg gtcggatgac gctgaagctg gcgtgtaata tagacctgtg cagcctcact
    30601 accgtcgcgc gcttcgatga tatcagcaag catcaattca gcatcggcac cggtgttctc
    30661 ttccaccgcg cgttgcagga attctgccca ttcggcagtt ttacccaact gctgatagca
    30721 ggtttgcaac atttccagcg tttcgctgac cagttctctg tcctgggata tgacgcgttg
    30781 caaactttcg acggctttgg cgtattctcc tttcgccata aacacgcgtc ccatcatgat
    30841 ggatacgcgg gcgctgtttt tatctgccgc cgcccctttt tttagcaagg tcatggcacg
    30901 atcgagatcg tcgctggcca tatgctgcag ggctaactca cagtagaaat gggcaatttc
    30961 gacgcgctgt ttatctttac ccagcttcac caggcgttcg gcaacatcaa ttgctttctg
    31021 ccactcactg gtagcctggt agatttgtag caactgttgc agcgcgccaa tgcggaagtc
    31081 agtttcatcg gtcagctgat tgaacatgtc ttccgcgcgg tcgtataacc cggcggccat
    31141 gtaatcacgc cccagttgtt gaatcgccaa cagacgctgt tcataggtca gcgaggcgct
    31201 ttccattagg gtctgatgga tgcgaatagc gcgatcaact tcgccacgcg aacggaacag
    31261 gtttccgagc gtaaggtggg cttcaacggt gcctgtatcc tctttaagca tatcgagaaa
    31321 caggtctacc gctttatcct gttgattact aagcaggaag ttaacccccg ctacgtaatc
    31381 acgcgacaag cggttggctt catcttgctt gttttgttgc gcacttctgc ggcccatata
    31441 ccagccatag gcagcggcta caggcaaaag cagaaacaac aactccagca tagaaagtta
    31501 ttccttcgcc gctgacgagt gcggcactac agccacgtca gtcgcgggtg aaagctggtt
    31561 ttccagtcgc tttattttac gttcagcgcg cgccagggaa acacgaactc gcagccagaa
    31621 caggccacaa atcaaccaac cgatagcaaa ccccgcagca aacaataccg ccagcaatgt
    31681 ggaaatacgg tactcccctt gcgctaacag ataattaaac gtcacctgtt gatcgttctg
    31741 cgcacccaac gtgaccgaaa tcacgaagat cgctaacacc agtaagaaaa tgagtaaata
    31801 tttcacatta cttcccgtta tgtggttaga gcgaataaat tatggtcaac ttaccgcaat
    31861 agccctttaa agtaccattt tcgcgccgtc tgcgaaatgg catccacgcg cattgttgat
    31921 gatttaaggc ctaaattcgg aaaatcaacc agcgctaact ttcttgttct cgttgtgcaa
    31981 tttcgcgatt ttcttccgca ggtggtgtta atggcccaca aattcgttgc gcaagccacg
    32041 ttgccaccgc caccagcgcc cacgaaatca acgtagctac caccagatcg cgtggccaat
    32101 gcatcccgag cagcaggcgg cttcccatga ctcccgttgc ccagaccaac aagatagcaa
    32161 tggttaacgt tcgccgacgc ggccacagca aaccaacggc cagcagtgcc caactggcag
    32221 caaacatcgt gtgaccggaa ggaaaggcaa accccgtctc tttctgccag tgtgaacgca
    32281 aatattgtgg aatatttttc tcttcagcca actgttcttt tactagattt ccgcgttctg
    32341 ctcgctttaa agtgtagaac tcatcaaccg gaatatgatg tattttttcc aaccagataa
    32401 caaaaggtcg tggttcctgg actttgtctt tgatccagga tttaacgcct tgtccaacaa
    32461 ggattgccgc cgccagaatg gcaaataata caaaggcagc cttaatgcga aaacgcagac
    32521 accagagaaa ccagccgaac aaaatcaaat gtgtaatgac gccccagggc tgggtgacag
    32581 tttcagtaac ccaaaaagcc gcttttagta gccaactttg ttctccaggt tgccaacgcc
    32641 agccagaaat ccatacggct actggcatga caagcaatag tgcagctccc actgcggtac
    32701 gtcgggcaat cgaacgcatg gcctctcctt tttgataagt cccacaatca taactgaaaa
    32761 tgccagttcc aagaaaaatt gacagatttg tgccattccg tgaacgatcg acgcgtcgtg
    32821 attaggtgaa ccccttctcg ttatggcaaa ataagccaat acagaaccag cattatctgg
    32881 agaatttcat gcagcttaaa cgtgtggcag aagccaaact gccaacccca tggggcgatt
    32941 tcctgatggt gggatttgaa gaactggcaa ccggacacga tcatatcgcg ctagtctatg
    33001 gcgatatttc cgggcatacc ccggtacttg cgcgcgtcca ttccgaatgt ctgaccggtg
    33061 acgccctgtt cagcttgcgc tgcgattgtg gcttccagct cgaagcggca ttgacgcaaa
    33121 ttgccgagga aggccgtggt attttgctgt atcaccgtca ggaaggtcgt aacattggtc
    33181 tgctgaataa aatccgcgct tacgcactgc aggatcaagg ttacgatacc gtagaggcta
    33241 accaccagtt aggcttcgcc gctgatgagc gcgacttcac tctttgcgct gatatgttca
    33301 aactccttgg cgtcaatgaa gtccgcttgt taaccaataa cccgaaaaaa gtcgaaattc
    33361 tgaccgaagc agggattaat attgttgaac gcgtaccatt gattgtaggt cgtaacccca
    33421 ataacgaaca ttatctcgat accaaagccg agaaaatggg ccatttgctg aacaaataac
    33481 cctcttgcat tgtgtaattc atttgcttgc cggaagcaaa ataaccggca agcaaatact
    33541 tgttacttca acatattacg aatgacataa tgcaaaatgc cgtcgttctg gtagtaggtc
    33601 aactccgtcg cggtgtcgat acgacaacgg cagggtacga cttcctggct accatccgcg
    33661 cgcgtaagcg tcaccggaac cgtcgcgcca ggttgtagat tttgcagatc gccaatatca
    33721 atcttctctt ccccggttag ccctaacgtt ttacgcgtca cgccttgcgg aaattccagc
    33781 ggcaggatgc ccatgccaat taaattcgaa cggtgaattc gttcaaacga ttcggcaatc
    33841 accacacgaa ttccaagcag acgaggtcct tttgccgccc agtcacggct tgagcctgat
    33901 ccatactctt tcccggcaat caccgccagc ggcgtttgct cctgcttata gcgcatcgca
    33961 gcatcataaa tagagactac gtcgctgtca ggtaaatgcc gcgtcatccc cccttcaacg
    34021 ccaggcacca tttcattacg gatgcgaata ttggcgaagg tgccgcgcat catcacttca
    34081 tggttaccac gccgcgaacc gtaggagtta aagtcttttc gctcaacacc ccgaccttgt
    34141 agatatcgac ccgctgggct gtcgggctta atactgcccg ccggagagat atggtcagtg
    34201 gtgactgaat cccccagcat tgcgaggatc cgcgcaccgt gaatatcttc cactggtgct
    34261 ggtgttgcct gcatttcatc aaagaaaggc gataagcgaa tataggttga gtcctcctgc
    34321 caaccgtagg tatcggatcg tgtgacgtta atttccttcc actctgctgt gccttcaaaa
    34381 acttctccgt actctttgcg gaacatttct gtggagactt gttctaccgc acgggcaatt
    34441 tcttgtgccg atggccagat atctttcaga taaaccggat cgcctttgcg atcatggccg
    34501 ataggctcag aagccaggtt gatattcata tttcccgcca gcacataggc aaccaccagc
    34561 ggcggcgagg ccagccagtt agttttaacc agcggatgga tacggccttc aaagttacgg
    34621 ttgccggaca gcaccgcacc gacggttaaa tcgccttttt tgattgctgt ttcgatagga
    34681 tcgggcagcg gcccagagtt accaatacag gtggtacaac cgtatcccac aaggttaaac
    34741 cccagttcgt cgagatacgg tgtcagtttc gcttttgcca gataatcaga aacgactttc
    34801 gaacccggtg ccagcgacgc tttgacccac ggttgccgct tgaggccaag agttacggct
    34861 tttttcgcca gcaagcctgc ggccatcagc acacttgggt tagaggtgtt ggtgcacgag
    34921 gttatcgcag caatgaccac agcgccatca ggtaactgat actgatgtcc gttcataaca
    34981 taatcgactg gcaggcgatc tttatgcgtg gcattcactt ccagttcgtt actggcggca
    35041 aatgcttttg gtacatcggg cagtgcaacg cgatcctgtg ggcgttttgg ccctgccagg
    35101 ctcgcttcaa cgtcattcat atccagttct aacatactgg taaaaatcgg ttcatcgccc
    35161 gggttacgcc acatgccctg cgctttggca tatttttcga ccaactcgac ctgatcttcg
    35221 ctgcgcccgc ttaaacgcat gtaatcgagg gttactgcat cgattgggaa gaagccacag
    35281 gtggcaccat attctggcga catattggca atggtggcgc gatccgccaa cggtagtgaa
    35341 tccagaccat caccataaaa ttcgacgaat ttccccacca cgccatgttt gcgcagcatt
    35401 tgggtaacag tgagaaccag gtctgtggcg gtaatacctt cacgtaattt tccggtaagt
    35461 ttaaagccca ctacgtccgg gataagcatg gaaaccggct ggcctaacat ggcggcttct
    35521 gcttcgatcc cacccacgcc ccaccccagc acgccaaggc cgttgatcat agtggtgtgc
    35581 gagtcagtac caacgagtgt atccggataa gcaatccatt caccgtcctg caattcgctc
    35641 cacactgctt tgccgagata ttcgaggtta acctgatggc aaatgcctgt gcctggcggc
    35701 acgacgctaa accgactgaa cgcttgcttt ccccatttca ggaacacata acgttcgtgg
    35761 ttgcgctcca tttccaggcg tacgttttct tcaaatgcct catcatcacc aaaacgatcg
    35821 acggtcaccg agtggtcaat gaccaggtcg accggtgaga gcgggttaac ctttgcagta
    35881 tcgccaccga ggcgtttaac cgcttcgcgc attgccgcca gatcaacaac ggcaggtacg
    35941 ccggtaaagt cctgcatcag cacccttgcc gggcggtagg caatttcacg gtcagcatgg
    36001 gcatttttca gccatcccgc cagtgcgtgg atatcctctt cggtaactga gttaccatcc
    36061 tgccagcgca gcaggttttc gagcaaaact ttgagtgact tgggtagacg ggtgatatcg
    36121 cccagtgatt tagcagcaag cggcaggctg tagtagtggt aagttttatc tttggcctgc
    36181 aacgtgtcct tactggcttc tcgtagggtt gacgacatag ctcctcctta atgacagggt
    36241 tgcgaagccc tgattctctt cagggtgaat attaaagata acacaaacat cgcgtaacga
    36301 tttgataaca acccaaattg ataaaagagg agaaaccttg tggcaaagca aatgacaacc
    36361 ccgccgcagc ggggtcaagg atcagtgagt aatgatccag aggagttcgg cccagaagag
    36421 catcgacaga gaaaacgcgc ccatccagga ccaatatttc atatttgtgt tgatcatagg
    36481 ctacaccctt aacggttatt attttgtgtt gagataactc aagctgttat tgctgcggtg
    36541 tattaaaagc cctttaattc attaaaaatg ggtaataaga atgatgataa ttattgtttc
    36601 ggctgatcat cttcagcaaa cagttctata aaggcttgct gctgcgatgt tagttgccag
    36661 tcgtctggaa taacgacgga acattcccgt ttcgtttctg gttcattgtc actatgctgg
    36721 cataatggtg acgactccag ttgctcctga ccgaccatag ttatccccac actttccaaa
    36781 tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag aaaaaccgcc agccaggctt
    36841 tacgttttaa ttcaggatca cggcggggtt cctgatttcc agaaggcatt gctaacctca
    36901 tataatcgcc atcacttatc agcaagacgt tgaatgattg gtgtaattaa tcatacgatg
    36961 agacaaatcc taaagaaact ttagtcaaaa atccagcgaa tttacgaatc gtctccagtg
    37021 aataattcaa tcttttgacc tgaaatagat atttgaaaac tggaatttgc gaggcgggta
    37081 attagacact actttacagg taatttaccc tataaatatg agggataaaa atattgtgca
    37141 cggtatagaa ataagtaaca aaagagaata tattccggca ccttcgctac ataaaaggtg
    37201 ccgaaaataa cgcaagaaat tatttttccg gcagttttat atctttaaac atgacctcaa
    37261 tttcttcatt ggagcgcaat gcgacagccg catcaacgac atcacgcgtt aaatgcggtg
    37321 caaaacgctg aatgaaatca tacatataac tgcgcaaaaa agtactacgg cgaaaaccaa
    37381 ttttggttgt actgtggctg aagatatcgt gagcatcaac gcgcacaagg tcgggatcgg
    37441 cgaccggatc caccgccatg ctggcaatga cccctacccc cagccctaac cggacgtaag
    37501 ttttaatgac gtcagcatcc gttgccgtga aaacgatacg cggcgttaac cctgcgcggt
    37561 taaaggcagt atccagttct gaacgtccgg taaagccgaa ggtatatgtc accaacggat
    37621 attgcgccag ttcttcaatg gtaatggctt ttttgcctgc cagcgggtga tccggagtga
    37681 ctacaatagc ccgattccag tggtagcacg gtaacatcac taaatcttca tacagatgca
    37741 gcgcttctgt ggcgatagcg aaatcggcat tgcctttaga gacggcatca gcaatttgtg
    37801 tcggcgagcc ctggtgcata tgcaaagaaa cgcggggata acgctcaata aagcctttga
    37861 taacgtttgg taacgcgtag cgtgcctggg tatgcgtggt ggcgatatac agcgagcctt
    37921 tatccggcca ggtgtgctca ccggcaaccg attttatggc atcgactttc gacaggactt
    37981 cgcgagcgat acgaattatt tcttgtcctg ctggcgttac ctgcgtcagg tgcttgccgc
    38041 ttcgggaaaa aatttgaatg cctagctcgt cttccagcat tctgacttgt ttactgatcc
    38101 cgggttgtga tgtgtaaagt ccttccgctg ttgatgagac attcaggtta tgattgacca
    38161 cctcaacaat atagcgaagt tgttgtaatt tcatgttaaa ccatccactt agacgccatc
    38221 agaacatcgt tttttcgtca cacctggcgg gtgactaata cgatttaata ataaaaaagg
    38281 tgctaactat aaccactata tcatttatag ccttagtgta taggtgtgaa gaaaaaataa
    38341 tatgcaataa aaaagggccg ctttcgcgac cctttgttta taaaaacctg acagaattaa
    38401 aggttatttt tttccttcaa cccatttgcc atcaacataa aatgctgacc agccagtcgc
    38461 ctttccgtct ttttccgaag agacatattg ctgtttggtt ttacggctaa agcgaaccat
    38521 ggtcttatta ccttccggat cctgctgtgg cgcatcggcc agataacgca gtttttccgg
    38581 cagacggtcg cggaagcgat aaagctcttc caccagtggc gcacgcgttt cacgcgattt
    38641 cgggaaagtg ttggcagcca ggaacacacc ggcagcaccg tcacgcagca cgaaataagc
    38701 atctgatttt tcgcacggca gctcaggtaa tggcaccgga tcttctttcg gtggtgccac
    38761 ttcgccgtta cgtaaaatct tacgtgtgtt tttacactct tcgttggtgc aggccatgta
    38821 tttaccgaaa cgccccattt tcaggtgcat ttcagagcca catttttcac actcaacgat
    38881 cgggccgtca taacctttaa tgcggaattc gccctcttcg atctcgtaac cgtcgcaggt
    38941 tgggttatta ccacagacat gcaacttacg tttcggatcg atgagatagc tgtccatcgc
    39001 cgtgccgcat ttcgggcaac gacgttttgc gcgcagcgcg ttggtttcag catcttcgcc
    39061 ttccagcacg ttcagcactt cgttttccgg caccaggtta atggtggttt tgcaacgctc
    39121 tttcggcggc agcgcatagc cagaacagcc aaggaatacc ccggtgctcg ctgtgcgaat
    39181 ccccattttg cgaccacaag tcgggcagtc aatgctggtc agaaccatct ggttcgggcg
    39241 cataccaccc tcttccggat ctttttcagc tttatctaac tgctgggtga aatccgagaa
    39301 gaagtgatcc agtacagctt tccactctgc ttcgtgattt gccacctggt cgaggctgtt
    39361 ttccatctgc gcggtaaagt cgtagttcat taactcgcgg aaattttctt caaggcgatc
    39421 ggtgacgatt tcgcccattt tttccgcata gaaacgacga ttttctactc gcacatagcc
    39481 acgatcctga atggtcgaaa tgatcgacgc ataggtagac ggacgaccga taccacgttt
    39541 ttccagttct ttaaccagcg atgcttcact gaaacgggct ggcggcttgg taaagtgctg
    39601 ggctggtgtt agttcaacga gcgtcagagc atcgccttta tcgactgccg gtaagatacg
    39661 atcttcatcg cctttacgca gtgcaggcat cacttttgtc cagccatcaa agcgcaaaat
    39721 acgaccgcgt gctttcaggc ggaaatcgcc cgcaccaacg gtcagcgtcg tggagtcata
    39781 tttcgctggg gtcatctggc aggcaacgaa ctgacgccag attaactggt acagtttctg
    39841 cgcatctgct tccatatcct tcagcgattc cgccatcaca ttgacgtcag aaggacgaat
    39901 cgcttcgtgc gcttcctgtg agttttcttt gctggcgtac tgattcggac tttccggcag
    39961 atatttctta ccaaaattat cgctgatata accgcgaacc atatttaccg cgtcctgact
    40021 caggttagtg gagtcggtac gcatgtaagt gatatagcct gcttcataca aacgctgcgc
    40081 catcatcatg gtttttttca cgccaaaacc aagacgggtg ctggcagctt gttgcagcgt
    40141 agaggtaata aaaggagcgc caggtttact ggttgtcggt ttgtcttcac gttccagcac
    40201 gctgtagcgc gctttttcca gcagacttac cgcagcctga gtttgttctt tgttgaccgg
    40261 acggaacggt ttgtcattct gatgagtcac ctgcaacgcc aacgcttcac cagatggcgt
    40321 ggtcgtgctg gcatcgactt cccagaactc ttccggtacg aacgctttaa tttcacgctc
    40381 acgctctacc accaggcgga ccgccaccga ctgcacacga ccggcagaca gaccacgagc
    40441 gatctttttc cacagcagcg gcgaaaccat ataccccacc acgcggtcca taaagcgacg
    40501 cgcctgctgg gcattaacac gatcaatatt cagctcaccc ggtttgttaa atgcctggcg
    40561 gatcgcgttt ttagtaattt cgttaaacac cactcggcta tagcgcgcat catcaccacc
    40621 aatcacttcc cgcaggtgcc atgcaatggc ttccccttcg cggtcaaggt cggttgcgag
    40681 atagatgtgg tcggcttttt cagccagttg tttcagttca gagacgacct tctctttacc
    40741 aggcaacact tcatagtgcg cctcccaatt gtgccacggg tcaaccccca tacggttgac
    40801 gagagcgcca cgttcatcct ttttaggctt tttagccgtc ttggtggagg tagagtcggc
    40861 actctttttg gcagctgagc cactggtcgg caaatcgcgg atgtgaccga cgctggattt
    40921 caccacgtag tcactaccca gatacttgtt gatcgttttg gcttttgccg gggactcaac
    40981 gatgacaaga gctttaccca tattcacctt acctaatttg attcttccag gaatgcgtcg
    41041 cacgttgata aaccttccac tggcaacgag ttaccgatat ggactcgctc tctgcggata
    41101 tcaacccctt gtcgtgactc tgttcacgaa aattcaggtg actgagttaa caggtcttgg
    41161 cctacaggcg ctatagcacg acggaatttt ggtcgaatgt caagcaaatc tattgccaga
    41221 ttgacgaaag cgtcacactg tacctgataa aatccgatac gcaactttat tagcatgcaa
    41281 aatccaaagt tgccagtaag gcgctcttgc caccagcgtg gtatatttgt ctgcaagcca
    41341 tgcctggcgt agactaatgt ccttgtttaa ggagtaaata atgaacaaag agacacaacc
    41401 catcgaccgc gaaacgctgt tgaaagaggc caacaaaatc attcgtgagc atgaggatac
    41461 gctcgcaggg attgaagcca ccggcgtcac gcagcgtaat ggcgtgctgg tatttaccgg
    41521 cgactacttt ttagatgaac aaggattgcc aacagccaaa agcaccgcgg tgtttaatat
    41581 gttcaaacac ctggcccatg tgctttcaga aaaatatcat ctggttgatt aaagcaaaac
    41641 gcgaggtctt agcctcgcgt ttgtctttta catcaatggc ttttgacccc gctgccacca
    41701 gcgtagcaac aatcgatcgg cgctctctgc cgcgctgccg gtgaatcggt caatgagtcg
    41761 tttacgctgc atatagcgta cattaaccac ttcacggcct tccatcaggc taagaataac
    41821 ttcatcactg gtgttgattt catcaaccag gcctttctct accgcctgtt gtccgtacca
    41881 gtgttcaccc gttgccacct gttcaatatc cagagacgga cgcatacgct tcacaaaatc
    41941 tttaaataac tgatgcgttt cgttcagctc ttcgcggaat ttctcccgcc cttcttcggt
    42001 attttcaccc agcaaggtca gcgtgcgctt atactgcccg gcggtgtgca gttcgatatc
    42061 aatatctttg cttttcagga agcggttaaa gttgggcatt tgcgccacca ccccaatgga
    42121 acccacaata gcaaacggtg cggaaacaat tttgtccgcc acacaggcca tcatgtaacc
    42181 gccgctggca gcgactttgt ctaccgtaac agttaaagga atgtttttat cacgcagacg
    42241 ctgcagctgc gaagccgcca acccgtaacc atgcaccatg ccaccagggc tttccagacg
    42301 gagcacaacc tgatcctgcg gtttgaatgc tgcgagtacc gccgttatct cttcacgtag
    42361 cgagttcact tcatgggcgt ccatgctgcc tttaaaatcc agcacccaga cgcgaggttt
    42421 actgtcagtt gccacctcgc ccagtttggc tttcgctttt gctgctttcg cttcttgctt
    42481 gtgctttttc ttctgcgctt tgtgccactg tttttgctga tgtgagtcca tcagcgccgc
    42541 ggccagttct tctttcatct ccttatactg ttcgctgaga ttgttgaccc gtaactcgcc
    42601 acgctggcgt ttattacgtt gagcaacatt gacaataatg gcggcaatcg ccgcaatcgc
    42661 tagcacaacg gtaacgattt tcgccaaaaa caaaccatat tcagacaaca attccacgag
    42721 tccccacctt ggttaaacaa cgctttggct cacagtttac aacaggcatg ttaagccgtc
    42781 tcgcagagat gttattgcag cgaaagtatg acaaagtatc cattttgcgg tgttatcgcc
    42841 ccaggctgat tgaaatgccc ttctgtttca ggcataaagc cccaaagtca taaagtacac
    42901 tggcagcgcg acagacaacg tcgttgagga gtagccatgc attaccagcc aaaacaagat
    42961 ttactcaatg atcgcattat cctggtgacg ggagccagcg atgggattgg tcgtgaagcc
    43021 gcgatgacgt atgcacgcta tggtgcgaca gtgattctgt tgggccgtaa tgaagaaaaa
    43081 ttacgtcagg tagccagcca cataaacgaa gaaactgggc gtcagccaca gtggtttatt
    43141 ctcgatttgc tgacctgcac gtccgaaaat tgccaacaac tggcacagcg cattgtcgtt
    43201 aattatccgc gtctggatgg tgttttgcat aatgccggat tgctcggcga tgtttgccca
    43261 atgagcgaac aaaatccgca ggtctggcag gacgtcatgc aggtcaacgt taatgccacc
    43321 tttatgctca cccaggcact gcttccttta ttactcaaat cggacgccgg ttcactggtc
    43381 tttacttcat caagcgttgg acgtcaggga cgagccaact ggggtgcata tgcagcgtcg
    43441 aaatttgcca ccgaagggat gatgcaggta ctggccgatg aatatcagca gcgcctgcgc
    43501 gtcaactgca ttaacccagg cggtacgcgc accgcaatgc gcgcaagcgc cttcccgacc
    43561 gaagatccac agaaacttaa aacacccgct gatatcatgc cgctctacct ctggctgatg
    43621 ggcgatgaca gccgccgtaa aaccggcatg acctttgacg cccaaccggg ccgtaaacca
    43681 ggaatttccc aatgagtgat gaacgctacc aacagcgtca gcagcgagtg aaagaaaaag
    43741 tagatgcccg tgtggcccag gcccaggatg aacgcggtat tatcatcgtc tttaccggca
    43801 atggaaaagg caaaaccacc gcggcatttg gtacggcaac acgcgcagtt ggtcacggaa
    43861 aaaaagtggg cgtcgtgcag tttattaaag gcacctggcc taatggcgaa cgcaatctgc
    43921 tggagccaca tggcgttgag tttcaggtga tggcaacggg ctttacctgg gatacacaaa
    43981 accgcgagtc tgataccgcc gcctgccgca aagtctggca acatgcaaag cggatgcttg
    44041 ctgattcctc actggatatg gttttgcttg atgaactgac gtatatggtg gcgtatgact
    44101 atttgccact ggaagaagtg gtgcaggcgt taaatgaacg tccacatcaa cagacggtga
    44161 ttatcacggg tcgaggctgt catcgggata ttcttgaact ggcggatacg gtaagtgagc
    44221 tgcgccccat cagacatgcg tttgatgccg gtgttaaggc gcaaataggg attgattatt
    44281 aatccggata tgacgtccgg catactttga ttaaccgtta ttacgcccgc cagaacgacg
    44341 accgccgctc acctgactgt gacgtttcac cgcacgacga atctgattag ccttcatgcg
    44401 acgacggtct ttttctacag cgactttaga gctggtttct ggcggaagct ccaccagttc
    44461 gcgcagatag ttagtctggg cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt
    44521 tggcagtggg atatcaccgt aacgaacacg gatcaggcgg ctcacctgca caccaaccgc
    44581 ttcccacaga cgacgaactt cacggttacg gccttcggtc agagtcacgt tgtaccactg
    44641 gttgatccct tcgccgccgc tgaacttgat ggttttaaaa gctgccggac catcttccag
    44701 ttgcacgcca cgactcaaat cacgcagttt cgcgtcgtca acctgaccaa atacacgcac
    44761 ggcgtattca cgttcaactt cacggcttgg atgcattaaa cggtttgcca gttcaccatc
    44821 ggtggtgaac agcagcagac cacaggtgtt aacgtccaga cgacctacgg caatccagcg
    44881 tgcgccacgc agctttggca gacggtcaaa caccgtcgga cgcccttccg gatcgttacg
    44941 ggtacacaac tcaccttccg gtttgtaata ggccagaacg cgacaaattt gttcagcgga
    45001 ctcacgtacc gaaatcagat gaccatcgat acgaattttc agcccagggg taacttcaac
    45061 gcgatcgccg agtttagcaa ttttgccatc aacactcaca cgaccggctt caataataga
    45121 ttcgatttca cggcgggagc cgtggccagc gcgggccagc actttctgta atttttcgct
    45181 catagagctt ccttttggtg tcgccttcac aggcgtcgaa caggagaatc aatggcgccg
    45241 tatagcgcca ttttaaggcc gcgtagtata cagagttacg cacttataag aaaggcttca
    45301 catcacctac gccttcacgc accacgaccg gcgtatcatc ggtaagatca ataaccgttg
    45361 ttggtttctg cccgagatag ccgccgtgaa taatcaaatc tacctgtttt tccagacggt
    45421 ctttaatttc ttcgggatcg gattcggtaa attcgctgcc tggcagcatt agcgaagtgg
    45481 aaagcatcgg ttcgcccagt gcttcaagca acgcctgcgc aatcgggtta gaaggcacac
    45541 gcataccgat ggttttgcgt ttctcctgca acagacggcg tggcacttcc ttcgtcccct
    45601 tcaggatgaa ggtatagttg cccggcgtgt tgtttttcat caaacgaaac gcaacattat
    45661 ccacgaatga ataggtcgag agttcagaaa gatcgcgaca catcagggta aagttgtgac
    45721 cgtccggcag ctggcgaata cgacaaatac gctccatggc gtttttatct tcaattttac
    45781 agccgagcgc atagccggaa tcagttggat agacaatcac cccgccttta cgcacgatct
    45841 ccaccgcctg gttgatcaga cgttgctgcg ggttatcagg atgaatataa aaaaactggc
    45901 tcataattcc ctctctgtgg tgttctgcgg ctgttcccat agctgccaga cgccttcaac
    45961 gcctgcgggc agccagagtt tacgacccag ttcgatccac gggcatggct gatgaaaatc
    46021 agatccttgt gatgcccata aatgatgctg acgcgcaagt gtggccagct gggtgcgttc
    46081 attgggcgat tgctggcact gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg
    46141 cgcaaccaat cttttcagcc atttagcact aagattgtac cgcccaggat gagctagcac
    46201 cgccttaccg ccagaatgat gaatgacatc aatagcttgt tctattgtac accactgtgg
    46261 cggaacgtat ccggttttcc cgcgcgccag atacttttta aagacatctg ccattgaact
    46321 ggctttgccg cactccacaa ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg
    46381 cgccagtcgt tgtgcgcctt ccagcgcccc aggtatttgc gctttttcaa gtctttcggc
    46441 aatttgctgc gcccgctgat tgcgccgttc tgtctgctgc gcgaggaact cacacattag
    46501 cggatgagta atatcaatat tcagcccgac aatatgaatt tcatgatttt cccagaccgt
    46561 ggaaatttcc acgccgggaa taagattcag cgccagtccc gaacgtgaaa tttcctctct
    46621 tgctggcgcg attgcagctg tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat
    46681 ctcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg
    46741 gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg
    46801 ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa tgagctgttg
    46861 acaattaatc atcgaaccag ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa
    46921 tgaaagcaat tttcgtactg aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc
    46981 accatgcgta aagcaatcag atacccagcc cgcctaatga gcgggctttt ttttgaacaa
    47041 aattagagaa taacaatgca aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc
    47101 gcttatcgcg acaacccgac tgcgcttttt caccagttgt gtgggaatcg tccggcaacg
    47161 ctgctgctgg aatccgcaga tatcgacagc aaagatgatt taaaaagcct gctgctgata
    47221 gacagtgcgc tgcgcattac agcattaggt gacaccgtca caattcaggc actttccggc
    47281 aatggcgaag ccctgctggc actactggat aacgccctgc ctgcgggtgt ggaaagtgaa
    47341 caatcaccaa actgccgtgt gctgcgcttc ccccctgtca gtccactgct ggatgaagac
    47401 gcccgcttat gctccctttc ggtttttgac gctttccgtt tattgcagaa tctgttgaat
    47461 gtaccgaagg aagaacgaga agcaatgttc ttcggcggcc tgttctctta tgaccttgtg
    47521 gcgggatttg aagatttacc gcaactgtca gcggaaaata actgccctga tttctgtttt
    47581 tatctcgctg aaacgctgat ggtgattgac catcagaaaa aaagcacccg tattcaggcc
    47641 agcctgtttg ctccgaatga agaagaaaaa caacgtctca ctgctcgcct gaacgaacta
    47701 cgtcagcaac tgaccgaagc cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt
    47761 tgtgaatgta atcagagcga tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg
    47821 attcgcgctg gagaaatttt ccaggtggtg ccatctcgcc gtttctctct gccctgcccg
    47881 tcaccgctgg cggcctatta cgtgctgaaa aagagtaatc ccagcccgta catgtttttt
    47941 atgcaggata atgatttcac cctatttggc gcgtcgccgg aaagctcgct caagtatgat
    48001 gccaccagcc gccagattga gatctacccg attgccggaa cacgcccacg cggtcgtcgc
    48061 gccgatggtt cactggacag agatctcgac agccgtattg aactggaaat gcgtactgac
    48121 cataaagagc tgtctgaaca tctgatgctg gttgatctcg cccgtaatga tctggcacgc
    48181 atttgcaccc ctggcagccg ctacgtcgcc gatctcacca aagttgaccg ttattcctat
    48241 gtgatgcacc tcgtctctcg cgtagtcggc gaactgcgtc acgatcttga cgccctgcac
    48301 gcttatcgcg cctgtatgaa tatggggacg ttaagcggtg cgccgaaagt acgcgctatg
    48361 cagttaattg ccgaggcgga aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat
    48421 ttcaccgcgc atggcgatct cgacacctgc attgtgatcc gctcggcgct ggtggaaaac
    48481 ggtatcgcca ccgtgcaagc gggtgctggt gtagtccttg attctgttcc gcagtcggaa
    48541 gccgacgaaa cccgtaacaa agcccgcgct gtactgcgcg ctattgccac cgcgcatcat
    48601 gcacaggaga ctttctgatg gctgacattc tgctgctcga taatatcgac tcttttacgt
    48661 acaacctggc agatcagttg cgcagcaatg gtcataacgt ggtgatttac cgcaaccata
    48721 ttccggcgca gaccttaatt gaacgcctgg cgacgatgag caatccggta ctgatgcttt
    48781 ctcctggccc cggtgtgccg agcgaagccg gttgtatgcc ggaactcctc acccgcttgc
    48841 gtggcaagct gcccattatt ggcatttgcc tcggacatca ggcaattgtc gaagcttacg
    48901 ggggctatgt cggtcaggcg ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg
    48961 acggtcaggc gatgtttgcc ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc
    49021 tggttggcag taacattccg gccggtttaa ccatcaacgc ccattttaat ggcatggtga
    49081 tggcagtacg tcacgatgcg gatcgcgttt gtggattcca gttccatccg gaatccattc
    49141 tcaccaccca gggcgctcgc ctgctggaac aaacgctggc ctgggcgcag cagaaactag
    49201 agccagccaa cacgctgcaa ccgattctgg aaaaactgta tcaggcgcag acgcttagcc
    49261 aacaagaaag ccaccagctg ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac
    49321 tggcggcgac gctggtgagc atgaaaattc gcggtgagca cccgaacgag atcgccgggg
    49381 cagcaaccgc gctactggaa aacgcagcgc cgttcccgcg cccggattat ctgtttgctg
    49441 atatcgtcgg tactggcggt gacggcagca acagtatcaa tatttctacc gccagtgcgt
    49501 ttgtcgccgc ggcctgtggg ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta
    49561 aatccggctc gtcggatctg ctggcggcat tcggtattaa tcttgatatg aacgccaata
    49621 aatcgcgcca ggcgctggac gagttaggcg tatgtttcct ctttgcgccg aaatatcaca
    49681 ccggattccg ccacgcgatg ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg
    49741 tgctggggcc attgattaac ccggcgcatc cgccgctggc gttaattggt gtttatagtc
    49801 cggaactggt gctgccgatt gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg
    49861 tggtgcacag cggcgggatg gatgaagttt cattacacgc gccgacaatc gttgccgagc
    49921 tgcatgacgg caaaattaag agctatcaat tgaccgctga agattttggc ctgactccct
    49981 accaccagga gcaactggca ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct
    50041 tgttacaagg taaaggcgac gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt
    50101 taatgcgcct gcatggccat gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac
    50161 tgcgcagtgg ttccgcttac gacagagtta ccgcgctggc ggcacgaggg taaatgatgc
    50221 aaaccgtttt agcgaaaatc gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc
    50281 agcaaccgct ggccagtttt cagaatgagg ttcagccgag cacacgacat ttttatgatg
    50341 cgctacaggg tgcacgcacg gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag
    50401 gcgtgatccg tgatgatttc gatccggcac gcattgccgc catttataaa cattacgctt
    50461 cggcaatttc ggtgctgact gatgagaaat attttcaggg gagctttgat ttcctcccca
    50521 tcgtcagcca aatcgccccg cagccgattt tatgtaaaga cttcattatc gacccttacc
    50581 agatctatct ggcgcgctat taccaggccg atgcctgctt attaatgctt tcagtactgg
    50641 atgacgacca atatcgccag cttgcagccg tcgctcacag tctggagatg gggctgctga
    50701 ccgaagtcag taatgaagag gaactggagc gcgccattgc attaggggca aaggtcgttg
    50761 gcatcaacaa ccgcgatctg cgtgatttgt cgattgatct caaccgtacc cgcgagcttg
    50821 cgccgaaact ggggcacaac gtaacggtaa tcagcgaatc cggcatcaat acttacgctc
    50881 aggtgcgcga gttaagccac ttcgctaacg gttttctgat tggttcggcg ttgatggccc
    50941 atgacgattt gcacgccgcc gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc
    51001 tgacgcgtgg gcaagatgct aaagcagctt atgacgcggg cgcgatttac ggtgggttga
    51061 tttttgttgc gacatcaccg cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg
    51121 cggcaccgtt gcagtatgtt ggcgtgttcc gcaatcacga tattgccgat gtggtggaca
    51181 aagctaaggt gttatcgctg gcggcagtgc aactgcatgg taatgaagat cagctgtata
    51241 tcgatacgct gcgtgaagct ctgccagcac atgttgccat ctggaaagca ttaagcgtcg
    51301 gtgaaaccct gcccgcccgc gagtttcagc acgttgataa atatgtttta gacaacggcc
    51361 agggtggaag cgggcaacgt tttgactggt cactattaaa tggtcaatcg cttggcaacg
    51421 ttctgctggc ggggggctta ggcgcagata actgcgtgga agcggcacaa accggctgcg
    51481 ccggacttga ttttaattct gctgtagagt cgcaaccggg catcaaagac gcacgtcttt
    51541 tggcctcggt tttccagacg ctgcgcgcat attaaggaaa ggaacaatga caacattact
    51601 taacccctat tttggtgagt ttggcggcat gtacgtgcca caaatcctga tgcctgctct
    51661 gcgccagctg gaagaagctt ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt
    51721 caacgacctg ctgaaaaact atgccgggcg tccaaccgca ctgaccaaat gccagaacat
    51781 tacagccggg acgaacacca cgctgtatct caagcgtgaa gatttgctgc acggcggcgc
    51841 acataaaacc aaccaggtgc tggggcaggc gttgctggcg aagcggatgg gtaaaaccga
    51901 aatcatcgcc gaaaccgggg ccggtcagca tggcgtggcg tcggcccttg ccagtgccct
    51961 gctcggcctg aaatgccgta tttatatggg tgccaaagac gttgaacgcc agtcgcctaa
    52021 cgtttttcgt atgcgcttaa tgggtgcgga agtgatcccg gtacatagcg gttccgccac
    52081 gctgaaagat gcctgtaatg aggcgctacg cgactggtcc ggcagttatg aaaccgcgca
    52141 ctatatgctg ggcaccgcgg ctggcccgca tccttatccg accattgtcc gtgagtttca
    52201 gcggatgatt ggcgaagaaa ccaaagcgca gattctggaa agagaaggtc gcctgccgga
    52261 tgccgttatc gcctgtgttg gcggcggttc gaatgccatc ggcatgtttg ctgatttcat
    52321 caatgaaacc aacgtcggcc tgattggtgt ggagcctagt ggtcacggta tcgaaactgg
    52381 cgagcacggc gcaccgctaa aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc
    52441 gatgatgcaa accgaagacg ggcagattga agaatcttac tccatctccg ccggactgga
    52501 tttcccgtct gtcggcccac aacacgcgta tcttaacagc actggacgcg ctgattacgt
    52561 gtctattacc gatgatgaag cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat
    52621 catcccggcg ctggaatcct cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa
    52681 cccggataaa gagcagctac tggtggttaa cctttccggt cgcggcgata aagacatctt
    52741 caccgttcac gatattttga aagcacgagg ggaaatctga tggaacgcta cgaatctctg
    52801 tttgcccagt tgaaggagcg caaagaaggc gcattcgttc ctttcgtcac gctcggtgat
    52861 ccgggcattg agcagtcatt gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg
    52921 ctggagttag gtatcccctt ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc
    52981 actctgcgcg cctttgcggc aggtgtgact ccggcacaat gttttgaaat gctggcactg
    53041 attcgccaga aacacccgac cattcccatt ggcctgttga tgtatgccaa tctggtgttt
    53101 aacaaaggca ttgatgagtt ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg
    53161 gttgccgatg tgccagttga agagtccgcg cccttccgcc aggccgcgtt gcgccataat
    53221 gtcgcaccta tcttcatctg cccgccaaat gccgatgacg acctgctgcg ccagatagcc
    53281 tcttacggtc gtggttacac atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac
    53341 cgcgccgcgt tacccctcaa tcatctggtt gcgaagctga aagagtacaa cgctgcacct
    53401 ccattgcagg ggtttggtat tttcgccccg gatcaggtaa aagcagcgat tgatgcagga
    53461 gctgcgggcg cgatttctgg ttcggccatt gttaaaatca tcgagcaaca tattaatgag
    53521 ccagagaaaa tgctggcggc actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc
    53581 agttaatccc acagccgcca gttccgctgg cggcatttta actttcttta atgaagccgg
    53641 aaaaatccta aattcattta atatttatct ttttaccgtt tcgcttaccc cggtcgaacg
    53701 tcaacttacg tcatttttcc gcccaacagt aatataatca aacaaattaa tcccgcaaca
    53761 taacaccagt aaaatcaata attttctcta agtcacttat tcctcaggta attgttaata
    53821 tatccagaat gttcctcaaa atatattttt cctctatctt ctcgttgcgc ttaatttgac
    53881 taattctcat tatcgactaa ttttaatgag tgtcgacaca caacactcat attaatgaaa
    53941 caatgcaacg caacgggaga aataacatgg ccgaacatcg tggtggttca ggaaatttcg
    54001 ccgaagaccg tgagaaggca tccgacgcag gccgtaaagg cggtcagcat agcggcggta
    54061 attttaaaaa tgatccgcaa cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg
    54121 gtggtaataa atcaggcaaa tcctgattca ccgttaatta ttaatatcag ctgataaaga
    54181 taattgcatc tgcgggccgt atggctcgca gtgatttcac tggagaaaaa tatgaatatg
    54241 aagaccattg aagatgtatt tattcaactg ctttcagata cctacagcgc agaaaaacaa
    54301 ttaacccggg cactggcaaa actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct
    54361 tttcatgcgc acctcgagga aactcatgga cagattgaac gaattgatca agttgtggaa
    54421 tcagaatcaa atctgaaaat taagcgcatg aaatgtgtgg caatggaagg tcttattgaa
    54481 gaagctaatg aggtcatcga aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt
    54541 gccgcagcac agaaagtcga gcattatgag attgccagtt acgggacatt agcgacgctg
    54601 gctgaacaat taggttaccg taaagcagcg aagcttctga aagaaaccct ggaagaagaa
    54661 aaggccaccg acatcaaact gactgatctg gcccttaata acgtaaataa gaaagccgaa
    54721 aataaagcct gaaatatgaa ttttaacttt tagtcatttt ataaagagga cattttcatg
    54781 aatcgtattg aacattatca tgactggtta cgtgacgccc acgcaatgga aaagcaagcc
    54841 gaatctatgc ttgaatccat ggccagccgt atagataatt atcctgaact acgcgctcgt
    54901 attgaacaac atcttagtga aaccaaaaac cagattgttc aactggaaac tattcttgat
    54961 cgtaatgaca tttcacgttc agtcattaaa gattccatga gtaaaatggc tgcgcttggg
    55021 cagtcaatcg gtggtatatt cccttctgat gaaatagtta aaggctctat tagcggatat
    55081 gtcttcgagc aatttgaaat cgcctgttac acctcactat tagcagcagc aaaaaatgcc
    55141 ggtgatacag tctcaattcc gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc
    55201 gactggctga ttcagcatat tccgcaaaca actgagaaat ttttaattcg ttctgaaact
    55261 gatggcgtag aagcgaagaa ataatcccct ttataccatg tccttattga ccccgtatat
    55321 tacggggtcg tttttgtgcg gaattaaaaa cgatatcctg ctgagaacat aaacacccac
    55381 ggatcgaggc gtacgctatc gtgttgctgt gcaccgccta gcttataatt ggcggtggta
    55441 tcgatatcca tgtaccacac tgacatgtta accaaccagt cacggttaat cagataatca
    55501 acccccacct gcccggcagc tccccaggaa tctttcagac tgagatcgga aagccccgcc
    55561 tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat accggcccca
    55621 acgtaaggac ggaatttgct gctggcatca ccaaaatacc actgcgccat cagtgttggc
    55681 ggcagatggt gaacggttgc aatatcgccg gtcgcccggg tgccgatttt atggcggaac
    55741 ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag
    55801 cccagttgag tgttattggt cacgctgaat ccacccagac ttcctaacgt accaccagca
    55861 ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc
    55921 gcaaaggcac tgccagaaag aagagttgtt actgccaaag ccgccactgt taactttttc
    55981 atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat tggagtaaca
    56041 aatgatccta catagatcac attacatatg ataattcacc atttattgat ctggattaat
    56101 ttagaagttg cattgagaat cctggttaat gtgatttaaa tcaattttta aatattttct
    56161 ctacgcaaat attaactcga tcgctacttg ccgcggtttg cccacgcctg aaggccagca
    56221 attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg ccacccgagg
    56281 cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag gagagtgcat
    56341 gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta atcaatttat
    56401 gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt
    56461 ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta gcggcagtag
    56521 tgggttgttc gacctggttc agaatatgtc accggaacag caacaaattt tgctgcaggc
    56581 ttcagcggcg tccacttttt cagaattaat cggtaacgcc attctcgccg gaggcgtaat
    56641 attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc
    56701 cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc ttttagtaca
    56761 gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac tggccctggc
    56821 accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta gcagtatgcg
    56881 gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt tgctggcaaa
    56941 aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa ttggtgccgt
    57001 actggcgaac accttgagca acctgatttc tgccatattg ctcatctatc tgttccgcct
    57061 gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa aatgaagcag
    57121 tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta tgacatctat
    57181 gcggctactg cagcgctgat cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc
    57241 tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt cttcggtggc
    57301 ttgacgctgt tcttccacaa tgatgagttt attaaatgga aggttacagt catttatgct
    57361 ctgtttgcgg gagccctgtt agtcagccaa tgggtgatga aaaagccgct aattcagcgg
    57421 atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg
    57481 gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg gctgccgcaa
    57541 aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat cttcacattg
    57601 ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta accagactgc
    57661 cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc atagtagcat
    57721 cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat aacgtccctc
    57781 agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc aatggtgaca
    57841 tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg gcaaaagaaa
    57901 ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta cggccggttg
    57961 cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg acatcggtca
    58021 gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg caacgctata
    58081 aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa cctcgcgcct
    58141 tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc tgcttactga
    58201 atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact gcctggctta
    58261 cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa catgttcgca
    58321 ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac gtcaaattta
    58381 actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta
    58441 cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt
    58501 gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac
    58561 tctacgggtt tgacatcgcg ttttggctgc tcctgtacct ttttcaccgg cttcggtttt
    58621 ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg cggttcgggg
    58681 atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg aacggcttgt
    58741 ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt
    58801 agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc accatgaatg
    58861 cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt tattgaagtc
    58921 ataatcattt cagtagaaaa accaagcctc gattttaaat gcaaatagca atcatattca
    58981 ataaggcaag acgatcagga tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt
    59041 atgcatgacc ttaacaaaga catcatcttt ccgttgcaga tcttcgccct ttcaaataac
    59101 gtactttaca actttcccga acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa
    59161 gacaaagctg atagcctcga aaaacgcctt tccgttcgtc cggcacattt agcacgttta
    59221 cagttactgc atgatgaagg tcgtttgttg acagctggtc caatgccagc ggtagacagt
    59281 aacgatccag gggctgcggg atttactggc tcaaccgtta ttgctgaatt tgaatcgctg
    59341 gaagcagctc aggcctgggc cgatgctgac ccgtacgtag cggcaggagt ctatgaacac
    59401 gtttcagtga aaccatttaa gaaagtgttc tgagtagaga tccaatattt aagggcacgg
    59461 tttttgcaac cgtgttcttg tttggccttt ccttatagcc ggtcagtgca ttcactacca
    59521 caagcagata ataactttat ctgtatttct ttatatgatg tgagcatgtt caggaaaaaa
    59581 gccctttcct gacgcagagt tgctcactca aaacctttga ttactgcgga taaagtcatc
    59641 tactattgtt taacacattc acgcgctgca tgtatgttca gaaaatgtgc acgctaagat
    59701 aattaaacaa aattgtggtt acagaggtat cagtgagtca ctgggctaca ttcaaacaaa
    59761 cagcaacaaa tttatgggtg accctacggc acgacattct cgcgctggcc gtctttttaa
    59821 atggattgct tatttttaaa acaatctatg gtatgtcggt caatttgctt gatattttcc
    59881 atatcaaagc attttcagag ttggatctct ccttgctggc aaacgcccca ctatttatgc
    59941 tcggcgtctt tcttgtcctg aactccattg gcttactgtt ccgggcaaag ctcgcatggg
    60001 caatcagtat cattttgttg ttgatagcgc taatttacac cctgcatttt tatccctggc
    60061 tgaaatttag tattggattt tgcattttta cgctggtgtt tttgctgata ctgcgcaaag
    60121 acttctccca cagtagcgcc gcagccggga caatttttgc atttattagt ttcacgacat
    60181 tactgtttta ctcaacctac ggtgcgcttt atttaagcga aggttttaat ccgcgaatag
    60241 aaagtttgat gaccgcgttc tatttttcga tagaaaccat gtcaaccgtc ggctatggag
    60301 atattgtccc tgtttctgaa tcagcacgat tgttcactat ttcggtcatt atttccggca
    60361 ttaccgtttt tgccacatct atgacctcaa tttttggtcc gcttatccgc gggggattca
    60421 acaaactttt aaaaggaaac aatcatacaa tgcatcgtaa agatcatttt attgtttgcg
    60481 gacattcgat tctcgccatc aatacgatcc tgcaactgaa tcaacgcgga caaaacgtaa
    60541 cggttatcag caacttgcct gaagatgata tcaagcaact tgagcaacgc ttaggcgata
    60601 acgctgatgt tatccccggt gacagtaatg acagttcagt attaaagaaa gcgggaatcg
    60661 atcgttgccg ggccattctg gcgctgagtg ataacgatgc agataacgcg tttgttgtac
    60721 tctcggcaaa agatatgagc agtgatgtca aaacagttct cgccgtcagt gatagcaaaa
    60781 acctgaataa gattaagatg gtacatccgg atatcattct ctcaccgcaa ctgtttggca
    60841 gcgaaattct ggcgcgagtt ttaaatggtg aagagattaa taatgatatg ctcgtttcaa
    60901 tgttgttgaa ctcaggtcat ggtattttta gcgataacga tgaacaagaa acgaaagctg
    60961 acagtaaaga atcggcgcaa aaatagagtt taaagacgga agagtaaact tattgggcac
    61021 cgcaataatg gtgcccagta catttagata ttgtagatcg caaacaataa cgagttacgc
    61081 tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg cgattgcccc
    61141 tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg ccccacttcc
    61201 gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat tatacccccg
    61261 cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat ccatatgcgt
    61321 aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt ttatacgttg
    61381 gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac tttacgcatt
    61441 ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat ttacattctg
    61501 ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg caaacgccgc
    61561 gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga ccttaaagcc
    61621 tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt caagctttgc
    61681 gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat gaccgagtca
    61741 gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa tattgagatg
    61801 gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc attaatggcg
    61861 gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag tgtggctttt
    61921 ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt cgaagcctta
    61981 aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca tcgcaagatg
    62041 atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga tcctcgctac
    62101 ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat ggaaggcccc
    62161 atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac cggaaaacgt
    62221 attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag cggtcacacc
    62281 attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca ggcattattg
    62341 actgcggctt attcggcgcg tgaatatttg atcatgacca cgccctactt tgtgccaagc
    62401 gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt cagtattatc
    62461 cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt ctttacggaa
    62521 ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca taccaagagc
    62581 gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat gcgtagtctg
    62641 tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc agaccttgcc
    62701 gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt atggctaaaa
    62761 cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc gttgctgtaa
    62821 aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa acaatcgcct
    62881 gactgaagat gaaacgcttg agcaggccta cgatattttt ctcgaactgg ctgccgacaa
    62941 tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg gcggcgcgga
    63001 gttattcgat ccggcggaag actggcagga acatgttgat ttcgatttga acccggactt
    63061 ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca acgatgtttt
    63121 cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca tctggcggga
    63181 ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta ttgtttttaa
    63241 agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag aacaggacgt
    63301 gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca accggaaggc
    63361 gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt cttctccaga
    63421 tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg tagtggattg
    63481 tggtagacct catcataggt ccccagttct actgcatggc cgagatacat caccaacaca
    63541 cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa tgacaatccc
    63601 atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga cacgtccagc
    63661 gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc acgagcaatc
    63721 ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat caggttaggc
    63781 aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg gcgtgacatt
    63841 ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat acgcgggttt
    63901 agcgatgcca acggctcctg gaaaatcatc tgaatatcac tgcgaacggc acgccattca
    63961 tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc ggtcgccttg
    64021 accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc ccctaccaca
    64081 cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt gagcgttttc
    64141 ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt aagatcggcg
    64201 atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc tccaccggtt
    64261 taaagcaagc acgcagacgg ccaggcgtaa actcttccag cggcggtgcg ctactacaaa
    64321 tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat cgcagcaagt
    64381 ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga cgcggcaccg
    64441 cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg cgcgcattgc
    64501 catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc cccgccacca
    64561 cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc agttcattca
    64621 atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt tcatccgcaa
    64681 tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga cgcatgccgc
    64741 cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt tttaccgcat
    64801 cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta tgcagcatca
    64861 gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc attgggtcct
    64921 gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct ggcaaattga
    64981 ggatttcacg cccattgaag gttgccgctc cgccaatgcg tccgttggca gccaacaggc
    65041 ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca atgcccagcg
    65101 tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg ccgtccgggg
    65161 tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt tgttgtgcga
    65221 gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat ctttcgggtc
    65281 gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca ccacaaggaa
    65341 tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc catcactcag
    65401 caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc taaggaaaga
    65461 ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca caccgagtac
    65521 gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa cttgtgccgc
    65581 ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac gagccatatc
    65641 cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc cgaaaaaggt
    65701 caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca gcagacgcat
    65761 cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa gtgtccccac
    65821 gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc cgctaatcgc
    65881 aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac cggactccat
    65941 atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact gggaaagcat
    66001 cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg caaccgccgc
    66061 acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt cgacctccag
    66121 cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca tcgcgagctc
    66181 cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac aatggcatta
    66241 aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga atagtcacgg
    66301 ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat ggtttcgatg
    66361 accatagaac cggtaataat gccgacaaat gcggggccca tataggagag cacgggtaac
    66421 agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc tttcgcccgg
    66481 gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt aatacgcgca
    66541 atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa tttaagcgcc
    66601 ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat catgactaat
    66661 aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc catcacggta
    66721 tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac acccagtatt
    66781 accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact ggatgccacc
    66841 aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc atgcgccagt
    66901 tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata tttcgcttcg
    66961 atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct tcccggcgcg
    67021 aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat cgcttccaga
    67081 cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct tctgtaatgc
    67141 gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc attagtgctt
    67201 cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg tatagccacc
    67261 aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa tggccgaatc
    67321 cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct cgtcagtcac
    67381 tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg cggtattcat
    67441 cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt ctgcacacca
    67501 gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg ttttccactc
    67561 ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag aggcagcaat
    67621 cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca acggtttgtc
    67681 tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt tttcctggct
    67741 ccagccaaac cattctggct gagtcaattt tgcgccagca gtatacggtg gagtgtaacc
    67801 ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc ggtccatacc
    67861 tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt tgttaatttc
    67921 gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct cttttttcag
    67981 cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac cactacggta
    68041 gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt taataacggt
    68101 tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt ttacgaccca
    68161 atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc atttttcgcc
    68221 gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa gtaatttata
    68281 gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa tagctttcac
    68341 gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg cgatatgccc
    68401 atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac gttgccagct
    68461 atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat ctttacgcaa
    68521 atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc caggtgctgg
    68581 atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga tattagactc
    68641 cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat tgttacgtac
    68701 cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca gcgcgacatt
    68761 ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg tgatgttggt
    68821 cattgttttt tggactccct cattataatt actggtatta accagcatgt gtaatcccgg
    68881 attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat cgttacgtat
    68941 tactatcacc gactttattt attctggtga ttcagacgat caccttatgt cgattcgtac
    69001 tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg ttaattattc
    69061 tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc ggttcttact
    69121 cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgtccgta tcagaatcta
    69181 tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag attggctaag
    69241 catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt taacaattga
    69301 ttattattta gcactttcct ctaccaaagg cagtggaatt actaatatca tttcaattat
    69361 tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag gagaagcagt
    69421 taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta ttccttttgc
    69481 ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga gcatggcagc
    69541 tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt aataccagta
    69601 acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt aatcacgttg
    69661 atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa tccccagcaa
    69721 cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat gctgtgataa
    69781 cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa cggcaacgcc
    69841 aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg cttgttctgt
    69901 ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac caggatgccc
    69961 ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt gtcgccgaga
    70021 aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt agttttgttt
    70081 cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat ccctaccggg
    70141 ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc aaaaagggtc
    70201 tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat aaggttgaaa
    70261 agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac ttaaaattgc
    70321 tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac caaatcatac
    70381 tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt atcagctttg
    70441 caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt gcttacgcca
    70501 cctggaagtg acgcattaga gataataact ctaatgttta aactctttta gtaaatcaca
    70561 gtgagtgtga gcgcgagtaa gcttttgatt ttcatcggtt aagcaaatca tcaccgcact
    70621 gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt atcaggagag
    70681 cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg taaaaaaagc
    70741 ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc gcgccgccgc
    70801 tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg aatccggcat
    70861 gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata tctacaacgc
    70921 ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg gtaccatcac
    70981 tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc cgacttcaac
    71041 tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct tctccccgca
    71101 cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg ctgctatcgc
    71161 tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg aactgtctaa
    71221 cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc cgggcatggt
    71281 taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta acactccagt
    71341 tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga tgtccaaaac
    71401 cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg actctgttta
    71461 tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg gtaaagagct
    71521 gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta tcgttggtca
    71581 gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca ccaagattct
    71641 gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa aactgtcccc
    71701 gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag cagagaaact
    71761 ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg ataaccaacc
    71821 ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga ttaacacccc
    71881 ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt ccctgactct
    71941 gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga aacacctgat
    72001 caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac ttccgaaatc
    72061 tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta ctgatggcca
    72121 caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg ctgatcagat
    72181 cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag tagaagcgga
    72241 cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca aaccagacgt
    72301 gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt gggttatgta
    72361 cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata tccgtaaacg
    72421 tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca ccaccacttc
    72481 tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta ctggtcagaa
    72541 atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg ccaacctggt
    72601 tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg gacgcagtaa ctcacgccat
    72661 ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc tgcaggcact
    72721 gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa atccggtagc
    72781 gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga acgccttcct
    72841 gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc cgcacggtct
    72901 ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca acccgaccaa
    72961 gcagactgca ttcagccagt acgaccgtcc gcaggctcgc cgtcgttatg ctgaaattgc
    73021 cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg agaaactgct
    73081 ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc gtgaagctgg
    73141 cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg cattcgatga
    73201 ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac agattctgct
    73261 ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa aagaagccgc
    73321 tccggctaaa gctgagaaaa aagcggaaaa atccgcttaa tcagtagcgc tgtctggcaa
    73381 tataaacggc cccttc
//
LOCUS       DADLSE010000004        64963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLSE010000004 DADLSE010000000
VERSION     DADLSE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.004"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN692_000257"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="JN692_000257"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7262668.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN692_000258"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="JN692_000258"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7262669.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN692_000259"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="JN692_000259"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7262670.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="JN692_000260"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="JN692_000260"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7262671.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="JN692_000261"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="JN692_000261"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7262672.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDTLGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCCDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /gene="cptA"
                     /locus_tag="JN692_000262"
     CDS             7826..9559
                     /gene="cptA"
                     /locus_tag="JN692_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7262673.1"
                     /translation="MHSTEVQAKPLFSWKTLGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQAMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="JN692_000263"
     CDS             9708..10559
                     /locus_tag="JN692_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7262674.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="JN692_000264"
     CDS             complement(10546..10887)
                     /locus_tag="JN692_000264"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7262675.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="JN692_000265"
     CDS             complement(10889..11767)
                     /locus_tag="JN692_000265"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7262676.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="JN692_000266"
     CDS             complement(11733..14030)
                     /locus_tag="JN692_000266"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7262677.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNLRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="JN692_000267"
     CDS             complement(14081..14401)
                     /locus_tag="JN692_000267"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7262678.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15480)
                     /locus_tag="JN692_000268"
     CDS             complement(14416..15480)
                     /locus_tag="JN692_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7262679.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAL
                     IAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGALTNSL
                     TQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAIAAVGI
                     CIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVIPSIMV
                     GSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVLKSLAR
                     KNGSSTDEKEDDLDLDFEIN"
     gene            15789..18290
                     /gene="ptsP"
                     /locus_tag="JN692_000269"
     CDS             15789..18290
                     /gene="ptsP"
                     /locus_tag="JN692_000269"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7262680.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSL
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18302..18964
                     /gene="fsa"
                     /locus_tag="JN692_000270"
     CDS             18302..18964
                     /gene="fsa"
                     /locus_tag="JN692_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7262681.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVITNPSIIAASKESIWEALP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            18975..19754
                     /locus_tag="JN692_000271"
     CDS             18975..19754
                     /locus_tag="JN692_000271"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HAY7262682.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEITPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTHAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGWRRLTRCITA"
     gene            19739..20022
                     /locus_tag="JN692_000272"
                     /pseudo
     CDS             19739..20022
                     /locus_tag="JN692_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
     gene            20411..20620
                     /locus_tag="JN692_000273"
     CDS             20411..20620
                     /locus_tag="JN692_000273"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7262683.1"
                     /translation="MADGARQQIGSTLFYDPAYIQLTYPGGDVPQERGVCSDVVIRAL
                     RSQKVDLQKLVHEDMAKNFAEYPQK"
     gene            20660..20914
                     /locus_tag="JN692_000274"
     CDS             20660..20914
                     /locus_tag="JN692_000274"
                     /inference="COORDINATES: protein motif:HMM:NF018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7262684.1"
                     /translation="MPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLAHIGVV
                     SDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN692_000275"
     CDS             complement(20941..21879)
                     /gene="yijE"
                     /locus_tag="JN692_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7262685.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN692_000276"
     CDS             complement(21940..24120)
                     /gene="katG"
                     /locus_tag="JN692_000276"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7262686.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN692_000277"
     CDS             complement(24449..25339)
                     /gene="metF"
                     /locus_tag="JN692_000277"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7262687.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRSEMSYAICHTLGVRPGL"
     gene            complement(25688..28120)
                     /locus_tag="JN692_000278"
     CDS             complement(25688..28120)
                     /locus_tag="JN692_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7262688.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN692_000279"
     CDS             complement(28123..29283)
                     /gene="metB"
                     /locus_tag="JN692_000279"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7262689.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEHALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29560..29877
                     /gene="metJ"
                     /locus_tag="JN692_000280"
     CDS             29560..29877
                     /gene="metJ"
                     /locus_tag="JN692_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7262690.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30061..30669
                     /locus_tag="JN692_000281"
     CDS             30061..30669
                     /locus_tag="JN692_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7262691.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN692_000282"
     CDS             complement(30730..30942)
                     /gene="rpmE"
                     /locus_tag="JN692_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7262692.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31145..33343
                     /gene="priA"
                     /locus_tag="JN692_000283"
     CDS             31145..33343
                     /gene="priA"
                     /locus_tag="JN692_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7262693.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLHLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33499..34524
                     /gene="cytR"
                     /locus_tag="JN692_000284"
     CDS             33499..34524
                     /gene="cytR"
                     /locus_tag="JN692_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7262694.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALCRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34616..35575
                     /gene="ftsN"
                     /locus_tag="JN692_000285"
     CDS             34616..35575
                     /gene="ftsN"
                     /locus_tag="JN692_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7262695.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADTPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35668..36198
                     /gene="hslV"
                     /locus_tag="JN692_000286"
     CDS             35668..36198
                     /gene="hslV"
                     /locus_tag="JN692_000286"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7262696.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36208..37539
                     /gene="hslU"
                     /locus_tag="JN692_000287"
     CDS             36208..37539
                     /gene="hslU"
                     /locus_tag="JN692_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7262697.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLLPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKMKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37606..38532
                     /gene="menA"
                     /locus_tag="JN692_000288"
     CDS             37606..38532
                     /gene="menA"
                     /locus_tag="JN692_000288"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7262698.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38625..39110
                     /gene="rraA"
                     /locus_tag="JN692_000289"
     CDS             38625..39110
                     /gene="rraA"
                     /locus_tag="JN692_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7262699.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRILVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN692_000290"
     CDS             complement(39195..39440)
                     /gene="zapB"
                     /locus_tag="JN692_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7262700.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39866..40711
                     /gene="glpF"
                     /locus_tag="JN692_000291"
     CDS             39866..40711
                     /gene="glpF"
                     /locus_tag="JN692_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HAY7262701.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPFDICVVEEKETTTPSE
                     QKASL"
     gene            40734..42242
                     /gene="glpK"
                     /locus_tag="JN692_000292"
     CDS             40734..42242
                     /gene="glpK"
                     /locus_tag="JN692_000292"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7262702.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKTVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42472..43482
                     /gene="glpX"
                     /locus_tag="JN692_000293"
     CDS             42472..43482
                     /gene="glpX"
                     /locus_tag="JN692_000293"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7262703.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43579..44325
                     /gene="fpr"
                     /locus_tag="JN692_000294"
     CDS             43579..44325
                     /gene="fpr"
                     /locus_tag="JN692_000294"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7262704.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN692_000295"
     CDS             complement(44330..44758)
                     /gene="uspD"
                     /locus_tag="JN692_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7262705.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44785..45084)
                     /locus_tag="JN692_000296"
     CDS             complement(44785..45084)
                     /locus_tag="JN692_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7262706.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45296..45736)
                     /locus_tag="JN692_000297"
     CDS             complement(45296..45736)
                     /locus_tag="JN692_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7262707.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            45837..46436
                     /locus_tag="JN692_000298"
     CDS             45837..46436
                     /locus_tag="JN692_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7262708.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46544..47311
                     /gene="tpiA"
                     /locus_tag="JN692_000299"
     CDS             46544..47311
                     /gene="tpiA"
                     /locus_tag="JN692_000299"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7262709.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN692_000300"
     CDS             complement(47366..48121)
                     /gene="cdh"
                     /locus_tag="JN692_000300"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7262710.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48228..49217)
                     /locus_tag="JN692_000301"
     CDS             complement(48228..49217)
                     /locus_tag="JN692_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7262711.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN692_000302"
     CDS             complement(49537..50499)
                     /gene="pfkA"
                     /locus_tag="JN692_000302"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7262712.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN692_000303"
     CDS             complement(50680..51582)
                     /gene="fieF"
                     /locus_tag="JN692_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7262713.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKILAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN692_000304"
     CDS             complement(51731..52231)
                     /gene="cpxP"
                     /locus_tag="JN692_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7262714.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHKQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52381..53079
                     /gene="cpxR"
                     /locus_tag="JN692_000305"
     CDS             52381..53079
                     /gene="cpxR"
                     /locus_tag="JN692_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7262715.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53076..54449
                     /gene="cpxA"
                     /locus_tag="JN692_000306"
     CDS             53076..54449
                     /gene="cpxA"
                     /locus_tag="JN692_000306"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7262716.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN692_000307"
     CDS             complement(54555..55229)
                     /gene="yiiM"
                     /locus_tag="JN692_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7262717.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIALGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN692_000308"
     CDS             complement(55378..56361)
                     /gene="kdgT"
                     /locus_tag="JN692_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7262718.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN692_000309"
     CDS             complement(56621..57241)
                     /gene="sodA"
                     /locus_tag="JN692_000309"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7262719.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57526..58560
                     /gene="rhaT"
                     /locus_tag="JN692_000310"
                     /pseudo
     CDS             57526..58560
                     /gene="rhaT"
                     /locus_tag="JN692_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN692_000311"
     CDS             complement(58557..59495)
                     /gene="rhaR"
                     /locus_tag="JN692_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7262720.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN692_000312"
     CDS             complement(59479..60315)
                     /gene="rhaS"
                     /locus_tag="JN692_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7262721.1"
                     /translation="MTVLHSVDFFPSCNASVAIELRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            60603..62072
                     /gene="rhaB"
                     /locus_tag="JN692_000313"
     CDS             60603..62072
                     /gene="rhaB"
                     /locus_tag="JN692_000313"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7262722.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62069..63328
                     /gene="rhaA"
                     /locus_tag="JN692_000314"
     CDS             62069..63328
                     /gene="rhaA"
                     /locus_tag="JN692_000314"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7262723.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63570..64394
                     /gene="rhaD"
                     /locus_tag="JN692_000315"
     CDS             63570..64394
                     /gene="rhaD"
                     /locus_tag="JN692_000315"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7262724.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFAVTPLASALAL"
     gene            64404..64718
                     /gene="rhaM"
                     /locus_tag="JN692_000316"
     CDS             64404..64718
                     /gene="rhaM"
                     /locus_tag="JN692_000316"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7262725.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT        15822 a        17513 c        16630 g        14998 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcggga cactccagcg
     4081 atccagcacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgctgcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg tactgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatacgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgctgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttt
     7861 tagctggaaa accctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcaggc
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaaa tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cgctcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acgcccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc aggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccatga tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    15241 aatggagtaa ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    15301 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    15361 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    15421 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    15481 aagctgctcc cgggcttgca aaagcccatg tccatatttt tgatggagga aacaataggc
    15541 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag
    15601 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagcatgccg caaaacggcg
    15661 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    15721 tgtcacatgg caggccgtta ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    15781 gaaaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc
    15841 gtccggcaag ccacgttgaa acgctgtgta atactttttc atctcaaatt gagtggcata
    15901 acctgcgtac tgaccgtaaa ggcaacgcca aaagcgctct ggcgctgatt ggcaccgata
    15961 cgctggtggg tgataactgc cagctactga tttccggggc cgacgaacag gaagggcacc
    16021 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    16081 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    16141 tccgcgcccg caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt
    16201 tagatctcaa tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    16261 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    16321 acggtgctac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    16381 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    16441 gtgcgaatca cttttgcgaa gagttcgccc gttccagcag cagatacctg caagaacgtg
    16501 ccctagacgt acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct
    16561 tcccggcacc gggcaaactg acgcagtccg ccatttgtat ggctgatgaa ctgaccccca
    16621 gccagttcct cgaactggat aaaaatcacc tcaaaggatt gttactgaaa agcggcggca
    16681 ccacctcaca tacggtgatc cttgcccgtt cgttcaacat tccgacgctg gttggtgtgg
    16741 atattgatgc cctcactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    16801 cgattgtggt tgagccagga gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    16861 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    16921 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    16981 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    17041 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    17101 gacgcagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctaa
    17161 acattcccgc agaggcaaac ccgttcctcg gttatcgcgc cgtgcgtatt tatgaagaat
    17221 acgcgtcgtt atttaccaca caactacgct cgatcctgcg cgcctccgct cacggcagcc
    17281 tgaaaatcat gatcccgatg atctcctcac tggaagagat cttatgggtg aaagaaaaac
    17341 tggcagaagc caaacagcaa ctacgtaacg aacacattcc gtttgatgag aagatccagc
    17401 tcggcattat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    17461 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    17521 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    17581 attacgccgt gcaggcggtg catcgccagg gtaaatggat tggtctgtgc ggtgagctgg
    17641 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    17701 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    17761 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    17821 cgcaattccg catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg
    17881 aaagtgactg gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg
    17941 cgggccgctg ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt
    18001 tctctaccgg tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc
    18061 aatccaccat cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc
    18121 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    18181 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    18241 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    18301 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    18361 cccgattgcc ggggtgataa ctaacccgag cattatcgct gccagcaagg agtccatctg
    18421 ggaagcgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    18481 catgagccgc gacgcgcagg ggatggtgga agaagcgaag cgcctgcgcg acgctattcc
    18541 gggtattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    18601 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcagaag ggttattagc
    18661 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg
    18721 ggacggcatt cgtacggttc aggagctgca aacgctgtta gaaatgcacg cgccagaaag
    18781 catggtgctg gcagccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    18841 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    18901 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    18961 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    19021 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg
    19081 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    19141 gactggtagt agaaattacg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgtc
    19201 tgcgtggcat cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    19261 ccctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    19321 ctatcgcctc taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    19381 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    19441 tcgtcgcggg cgcacctgca cgtctgttag cagcgggtat cggcgatgcg ctggcaacct
    19501 ggtttgaagc gcgtgcttgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    19561 cccatgcggc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    19621 aagcgatgct tgccgccgaa cagcatgtag taaccccggc gctggagcgc gtgattgaag
    19681 cgaacaccta tttgagcggc gttggttttg aaagcggcgg ctggcggcgg ctcacgcggt
    19741 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    19801 atttggtacg ctgacgcagc tggtactgga aaacgcgccg gtggaggaaa tcgaaaccgt
    19861 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    19921 agatgtcccg gcgaaaatgc ccatgcgacg ccagatcagg tttacgccgc actgctggta
    19981 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20041 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20101 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20161 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20221 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20281 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20341 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatct aggccaatac
    20401 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20461 ggcgtatata cagcttacct atcctggcgg tgatgttccg caagaacgcg gtgtatgttc
    20521 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20581 agatatggcg aagaattttg ctgagtaccc gcaaaaatag aagttaaagc gcccggacag
    20641 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20701 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20761 agataacggg ctggcgcata ttggggtggt gtcagatggc ttcgcccgcg acggtacgcc
    20821 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20881 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    20941 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21001 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21061 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21121 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21181 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21241 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21301 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21361 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21421 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21481 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21541 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21601 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21661 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21721 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21781 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21841 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21901 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    21961 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22021 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22081 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22141 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22201 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22261 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22321 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22381 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22441 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22501 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22561 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22621 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg gcaccaccgc gtttgtcgcc
    22681 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22741 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22801 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22861 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22921 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    22981 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23041 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23101 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23161 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23221 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23281 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23341 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23401 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23461 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23521 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23581 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23641 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23701 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23761 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23821 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23881 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    23941 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24001 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24061 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24121 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24181 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24241 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24301 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24361 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24421 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24481 aaatcgcgta actcatttca gagcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24541 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24601 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24661 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24721 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24781 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24841 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24901 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    24961 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacgaata ccgttattcc
    25021 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25081 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25141 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25201 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25261 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25321 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25381 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25441 ccgaaaaatg accaattatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25501 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25561 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25621 tcaatatatt tcattagaaa ccatagaccc gaaaacatga gtaccgggca ttattaaatt
    25681 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25741 cgtcgcgccc agcaccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25801 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25861 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25921 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    25981 tttcaaagaa atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc gattccacac
    26041 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26101 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26161 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26221 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26281 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26341 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26401 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26461 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26521 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26581 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26641 gcacaaattc aaagccggta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26701 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26761 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26821 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26881 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    26941 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27001 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27061 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27121 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27181 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27241 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27301 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27361 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27421 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27481 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27541 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27601 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27661 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27721 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27781 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27841 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27901 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    27961 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28021 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28081 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28141 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28201 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28261 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28321 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28381 actcaacatt gcgccaaaac ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28441 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28501 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28561 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28621 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28681 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28741 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28801 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28861 taccagtttg ggtttttctg ccagcgctgc ccgtaatgca tgttcatcgc cttgatcaac
    28921 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    28981 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29041 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29101 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29161 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29221 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29281 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29341 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29401 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29461 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag tacccacata aattgtgggg
    29521 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29581 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29641 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29701 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29761 gggcaacctt tgccagatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29821 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29881 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    29941 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30001 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30061 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30121 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30181 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30241 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30301 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30361 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30421 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30481 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30541 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30601 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30661 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30721 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30781 accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc tgcacacgtc
    30841 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30901 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    30961 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31021 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31081 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31141 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31201 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31261 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31321 gctaaaagcg gtagtcgaag tgctggatag tgagccggtg tttactcact ccgtctggcg
    31381 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31441 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31501 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31561 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31621 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31681 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31741 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31801 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31861 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31921 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    31981 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32041 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32101 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32161 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32221 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc acctgacgcg
    32281 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32341 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32401 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32461 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32521 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32581 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32641 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32701 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32761 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32821 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32881 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    32941 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33001 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33061 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33121 gatcctctcc agcccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33181 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33241 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33301 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33361 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33421 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33481 gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33541 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33601 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33661 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33721 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33781 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33841 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33901 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    33961 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34021 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34081 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgtg tcgctgcgga
    34141 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34201 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34261 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34321 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34381 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34441 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34501 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34561 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34621 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34681 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34741 cgttcttgtg acctttatcg gaggtctgta cttcattacg catcacaaga aagaagagtc
    34801 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34861 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34921 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    34981 tgaacaaatg caagctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35041 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35101 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35161 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35221 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35281 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacacg ccaaaaccga cggcggagaa
    35341 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35401 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35461 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35521 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35581 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35641 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35701 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35761 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35821 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35881 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    35941 cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac cggtaacggt
    36001 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36061 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36121 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36181 ttaagctaca aagcataagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36241 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36301 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36361 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36421 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36481 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36541 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag agctggcaga agaacgtatt
    36601 ctcgacgtgc tgctcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36661 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36721 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36781 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36841 aaagcgcgta agatgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36901 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    36961 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37021 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37081 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37141 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37201 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37261 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37321 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37381 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37441 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37501 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37561 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37621 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37681 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37741 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37801 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37861 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37921 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    37981 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38041 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38101 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38161 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38221 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38281 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38341 ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccatt actggtgaag
    38401 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38461 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38521 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38581 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa tcctatgaaa tacgatactt
    38641 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38701 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38761 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tattcttgtc gtcgatggcg
    38821 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38881 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    38941 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39001 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39061 ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaatga taagaaaggc
    39121 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39181 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39241 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39301 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39361 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac
    39421 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39481 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39541 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39601 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39661 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39721 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39781 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39841 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39901 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    39961 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40021 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40081 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40141 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40201 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40261 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40321 gatggtgatt accgctattc tgatggggct gatcctggcg ttaacggacg atggcaacgg
    40381 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40441 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40501 ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40561 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40621 caaactgatt ggtcgccatt tgcctttcga tatctgtgtt gtggaagaaa aggaaaccac
    40681 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40741 aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg
    40801 atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac
    40861 caggttgggt agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag
    40921 aagtgctggc gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc
    40981 agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg
    41041 tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag
    41101 attatatccg cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga
    41161 agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc acgtcgtggt gaattgctgt
    41221 ttggtacggt tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg
    41281 attacaccaa cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca
    41341 aaatgctgga agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg
    41401 aagtatacgg tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga
    41461 tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga
    41521 agaacaccta tggcactggc tgctttatgc tgatgaacac tggcgagaaa acggtgaaat
    41581 cagaaaacgg cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt
    41641 tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt
    41701 tgattaacga cgcctacgat tccgaatatt tcgccaccaa agtgcaaaac accaatggtg
    41761 tgtatgtggt tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg
    41821 gggcgatttt cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg
    41881 agtctattgc ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc
    41941 gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc
    42001 agtccgatat tctcggcacc cgcgttgagc gcccggaagt gcgcgaagtc accgcattgg
    42061 gtgcggccta tctcgcaggc ctggcggttg gcttctggca gaacctcgac gagctgcaag
    42121 agaaagcggt gattgagcgc gagttccgtc caggcatcga aaccactgag cgtaattacc
    42181 gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt
    42241 aatgtcaggc acctgaatcg attgcctgat gctgatcgcc tgatgcgacg cttacgcgtc
    42301 ttatcaggcc tacgagccaa tgccattttg tgtaggcggg ataaggcgtt cacgccgcat
    42361 ccggcatcac gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatccca
    42421 cccttctcct gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga
    42481 gaacttgcca tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg
    42541 ttaggacgcg gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg
    42601 ctcaaccagg tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca
    42661 ccgatgctct acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct
    42721 gttgatccga ttgaaggcac gcgcatgacg gcgatgggcc aggctaacgc gctggcggtg
    42781 ctggcagtag gcgataaagg ctgcttcctc aatgcgccgg atatgtatat ggagaagctg
    42841 attgtcgggc cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg
    42901 cgcaatgtag cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct
    42961 aaaccacgcc acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct
    43021 attccggacg gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac
    43081 gtgctgtacg gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca
    43141 ttagatggcg acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa
    43201 gagaatcgtc gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc
    43261 ggtaaagtat tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgccacc
    43321 ggtattacca aaggcgatct gctggaaggt attagccgca aaggcaatat cgcgactacc
    43381 gaaacgctgc tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat
    43441 ctggatcgca aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag
    43501 ccttccagtc cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa
    43561 tcaaaacagg agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac
    43621 tggaccgacg ccctgtttag tctcaccgtt cacgcccccg tgcttccgtt taccgccggg
    43681 caatttacca agcttggcct tgaaatcgac ggcgaacgcg tccagcgcgc ctactcctat
    43741 gttaactcgc ccgataatcc cgatctggag ttttacctgg tcactgtccc cgatggcaaa
    43801 ttaagcccac gattggcggc actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca
    43861 gctggcttct ttgttctgga tgaagtaccg gactgcgaaa cgctatggat gctggcaacc
    43921 ggtacagcga ttggccctta tttatcgatt ctgcaactag gcaaagattt agatcgcttc
    43981 aaaaatctgg tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg
    44041 atgcaagaac tggaaaaacg ctacgaaggc aaactgcgca ttcagacggt ggtcagtcgg
    44101 gaaacggcag cggggtcgct cacgggacgg ataccggcat taattgaaag tggggaactg
    44161 gaaagcgcga ttggcctgcc gatgaataaa gaaaccagcc atgtgatgct gtgcggcaat
    44221 ccacagatgg tgcgcgatac acaacagttg ctgaaagaga cccggcagat gacgaaacat
    44281 ttacgtcgcc gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga
    44341 taaacggcac gatgagcaaa tccgcactca tcttattgat catcccgcga tatgccggca
    44401 tcaaacggtt gataaatgag tgatgatgac cacagacaag gaggtcgcac tgctcttttt
    44461 gcataatttc cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg
    44521 gccattgaat atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt
    44581 gaagaatatc ttctgttgca gggaagtaga tacccggata caactcgctt aagccatcat
    44641 caatatgaat taacgtcagg tgagcgtcat tatgtctggc gagctccagg gctttattca
    44701 ccagtaaggc atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt
    44761 ttaactcctt ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat
    44821 catctcggcc tggcatgaga aagtgaaggc cgcatcaagc tcaacttgcc cctccagatc
    44881 cttaatctta taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc
    44941 tttcagtttg gtcataaaag actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt
    45001 actggtacag ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact
    45061 gcatttatct acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac
    45121 gacttaagaa taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc
    45181 attttttttg atattgctta aggtgaagag ttcagaagga ttttccgggt attgtcattt
    45241 gcatcgtgga aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat
    45301 tacttgattt attgtcggct ttatacttca catcctgagt atctttacca tatttatttt
    45361 cgccttgtgt gccaacaaac gcaccaagat cgataagcat catcaccaga atcaacgtcg
    45421 ggacaaaacg ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca
    45481 gcagcatcca cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca
    45541 agcgcttaac agttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg
    45601 tctgaatatc gagtagattc ttacccgcca gtgagaaaag caccagcatg cctgcgaacc
    45661 acaggcctat ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc
    45721 attgctgtat ggtcatgtaa gttccttgat ggttgtcttt tcctggattc tacccttttg
    45781 acaagggcga cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga
    45841 agccagggtg tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc
    45901 atgcacaaac accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt
    45961 tcgatctctc catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac
    46021 tgaacgaatt tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa
    46081 gtaaaattaa cgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca
    46141 aaagcattca aatgacctgg ctacccatcc aggggccaga gcaaaaagca gcgaaagcga
    46201 aagctcagga atacatggca gcagtgatcc gcacactcac cccattaatg accaaaacac
    46261 aaagccagaa aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg
    46321 agacagaagg tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg
    46381 ctgttgaacc gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa
    46441 aagcaaagcc tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg
    46501 gcggccatct tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt
    46561 gatgggtaac tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct
    46621 gcgtaaagag ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta
    46681 catcgatatg gcgaagcgcg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt
    46741 ggacctgaac ctgtccggcg cattcaccgg tgaaacttct gccgctatgc tgaaagacat
    46801 cggcgctcag tacatcatca tcggccactc tgaacgtcgt acttaccaca aagagtctga
    46861 cgaactgatt gcgaaaaaat tcgcggtgct gaaagagcag ggtctgactc cggttctgtg
    46921 catcggtgaa accgaagctg aaaacgaagc gggaaaaact gaagaagttt gcgcacgtca
    46981 gatcgacgcg gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta
    47041 cgaacctgta tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt
    47101 tcacaaattc atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat
    47161 cattcagtac ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccaga
    47221 catcgacggc gcgctggttg gcggtgcttc tctgaaagct gacgctttcg cagtgatcgt
    47281 taaagctgca gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg
    47341 cacttacttt ccttaactcc tcgccttaac gcaaaatctc acactcatga tcctggattt
    47401 cctcggctga agcacggttc agcgtcagta gatttcgttg tgtcgccagc aacacaaacg
    47461 aattatcact ctgccgtacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag
    47521 gcacttcttc tgcaagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc
    47581 gacgcgccaa gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga
    47641 tgttcgccag atttttatcc agttgttcgc gcacatcagg acgaatacaa gagatatgaa
    47701 tatgaaaatg gttttgcgta cgcccggtgc gggagttgat cgccaaagaa accgcgcgat
    47761 cgggaaccgg ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa
    47821 agaagttcgg cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg
    47881 ttggcatcaa cagatattgc agtggaccat taagatcttt taaaaccacg tatccggcat
    47941 tgggtttgac ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt
    48001 cctcaaggac aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa
    48061 taccggcagc cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca
    48121 tctttttttc cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa
    48181 aacccggtcg cgagaaacca ccgggtttga taattatcct agggaaatca gcgtttgctg
    48241 atctgatcga acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg
    48301 aactcttcat caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgct
    48361 acttcagcgt cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag
    48421 tagagatatt tcaggtaggc ttccgccacc tctttggtgc cttttttctc gaccacttta
    48481 tcgaccaccg acaccgttgg ctctgcgagg atagactcac tcggcgtgac gatttcgaat
    48541 ttatctttcc ccagttcatt cgctgccagc agagcttcgt tttcccaggc gatcagtaca
    48601 tcgccaatcc cgcgctcgac aaaggtgtta gtggagccgc gcgcgccaga atccagaact
    48661 tcgacgtttt tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg
    48721 tgatgcagcg cgtagcccca ggcagccagg tagttccagc gtgcgccacc agagcttttc
    48781 ggattaggcg tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc
    48841 ttcggattgc ccttacgcac caggaaaaca atggtggaag tgtacggtgc ggagttatcc
    48901 ggcagacgtt tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca
    48961 taggccagag ccagcgtgac aacatcagct tcaataccgt tgattaccga cgtcgcttgt
    49021 ttacctgaac caccgtgtga ctgacgaatc accacgttat caccagtttg ctgtttccag
    49081 tgggcgctga atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg
    49141 ttaagaagct gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct
    49201 acgccccact tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc
    49261 agaaggttaa ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt
    49321 tgagggatta aaaaggcgga agaaatccgc ctcatattgc tgacaaagtg cgctttgtcc
    49381 atgccggatg cggcgcgaac gccttatccg gcctacaaaa gtttgcaaat tcaataaatt
    49441 gcagaattca tgtaggcctg ataagcgaag cgcatcaggc atttttgctt ctgtcatcgg
    49501 tttcaggcta aaggaatctg cctttttccg aaatcattaa tacagttttt tcgcgcagtc
    49561 cagccagtca cctttgaacg gacgcttcat gttttcgata gcgtcgatga tgtcgtggtg
    49621 aaccagctgt tcgttctgga tacctacaca acgaccgccg taacctgcca acagcagatc
    49681 gatagcgtaa gcgcccatac gggaagccag aatacggtcg taaggcaccg gagaaccacc
    49741 gcgctggatg tggcccagca cagttgcgcg ggtttcacga ccggtttctt tctcgatgaa
    49801 atgcgccagt tcgtcaacat cacacatatg ttcggtaatc gccacgatcg cgtgtttttt
    49861 acctttcgcg atacccgctt tgatttcgtt taccaggtct tcacggctga attcaacttc
    49921 cggaacgaca acgaattcac agccaccggc aatggccgca gccaaggtca gatcgccaca
    49981 ataacggccc atcacttcca ccacggaaat acgctggtga gaagaagagg tgtcacgcag
    50041 acggtcgatc gcttctacaa cggtgctcag cgcagtgaag aaaccgatag tgtagtcagt
    50101 gcctttgatg tcgttgtcga tagtgcccgg cagaccgatg cacgggaagc ccatttcggt
    50161 cagacgcatt gcacccatgt aggaaccgtc accgccgata accaccagcg cgtcgatacc
    50221 acgttttttc aggttttcga tagccacggc gcggatgttc tcgtcgcgga attccgggaa
    50281 acgcgcagaa ccgaggaacg taccgccacg gttgatcatg tcagacacgc tgtaacggtc
    50341 tagctgtacc atacggtctt catacagacc cagatagccg tcataaatac ccattacttc
    50401 cagaccttct gtcagcgcag aacgaacaac cccgcgaatt gcggcgttca tgcctggcgc
    50461 atcaccgccg cttgtcaaca cacctatttt cttaatcatg actacctctg aactttggaa
    50521 tgcaaaatga aatctgttgc cggaagtctt cttgcacatc gaagtgatcc aacgaatgtg
    50581 caaatagtat aacaatcact tcctgctgaa ttgattcagg tcaggccaaa tggcggtatt
    50641 ttatacacaa aatgcgggtc tggctctctt ttatactgat tatgaaagca tagaccgttt
    50701 accctccctg ggtacgacgg aacaggggtc ctgatggata atcacatccg atcccggaaa
    50761 acgccgtaaa atagcctgct ctacctgatc cgccaccata tgtgcctgaa ccaaaggcag
    50821 agagtcttcc atttccaaat gaatctgaat aaagcgggtc ggccctgact gccgcgtgcg
    50881 aagatcgtga gcgccgctaa cacccggcca ggaagtcacg atatcaataa tttcttgccg
    50941 ttcttcatca ggcaatgcgc gatccagtaa tgactgtacc gcctcatatc ccatgcgtaa
    51001 cgcgctatat aaaatataga tgccgattcc caatgcaaac agagcatcgg cgcgatgcca
    51061 gccgtaccaa gacaacccca gcgccagcag aattgcgccg ttcatcataa catcagactg
    51121 gtaatgtagc atatcagccc gcaccgcctg gctttgcgtc cggcgcacca cccaccgctg
    51181 aaacgagaca aggataatcg tacaaattag cgccacaatt gtcacgataa ccccgacgcc
    51241 tggatctgtc atcggtgttg gagataccag atgttgaata cccgtcaaaa acaggaatag
    51301 tgccgaaccg gagataaaca tactttgcgc cagcgccgcg agggactcag ctttaccgtg
    51361 accaaacgag tgattatcgt cggcaggttg cagggaatat cgcaccacca gtaaattcgt
    51421 caacgacgcg ccgatatcca ccagcgaatc caccagcgcg gcgagaatac tcaccgaccc
    51481 ggtataccac catgccaaaa ttttaatcag caatagcaac gaagccatcg ccgtcgcagc
    51541 aatcgccgcc cgactgacca gccgtccata agattgattc ataaatactc ccgctatcaa
    51601 ctgacgctag tataacggaa gcaaatcatc tgcaatgcat taagcagcag gcaaattgag
    51661 gataaaaaaa aaccccacat catgtggggg aagacaggga tggtgtctat ggcaaggaaa
    51721 acagggttta ctactgggaa cgtgagttgc tactactcaa tagcttcaac gatgaacttt
    51781 tttgccattg cgtcacgtca cgcaactgct ccattcgttg ttgatgtttc tcgtttaaaa
    51841 ccgcttgctg ctccggcgtt aacaggcgat acatttggtt gcggactttt gccatctcaa
    51901 cctgacgagc aatttgctca ttcgccattt tttctgcctg tgcgcgcaca gcgttttcat
    51961 caaaattttc tgcggtgaca aggcgatgca ttgtctccag ttcgctaaca ttaacaggag
    52021 gctgtttgtg ccgggcctgt tgcataagat ctcgcatctg ctgacgctga tgttcggtta
    52081 aacttatgcc gtcgaacata tggctctgcg tactgcgctg cgtaagttct tcacccggat
    52141 gccagttatc gcctgaaccg acttcagcag cgtggcttaa tgaactgact gccagcgttg
    52201 aggccatgac ggcagcggta actatgcgca tcatttgctc ccaaaatctt tctgtcgcga
    52261 ttcaacgata gagagtttac gattcaggct gcaaacatgc gtcagggggt gtaaaacaac
    52321 gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctcggagg tatttaaaca
    52381 atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt aaaggagctg
    52441 ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc gcttgatctt
    52501 ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa aaatggtatc
    52561 gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt gacggcgcgc
    52621 ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta tctcccgaaa
    52681 ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg ttcgcactgg
    52741 agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga tgccttagtg
    52801 ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt aaccggtact
    52861 gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc ccgtgaacat
    52921 ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat tgatatgcac
    52981 atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg gtttaaaacc
    53041 ttgcgtggtc gcggctatct gatggtttct gcttcatgat aggcagctta accgcgcgca
    53101 tcttcgccat cttctggctg acgctggcgc tggtgttgat gttggttttg atgttaccca
    53161 agctcgattc acgccagatg accgagcttc tggatagcga acagcgtcag gggctgatga
    53221 ttgagcagca tgtcgaagcg gagctggcga acgatccgcc caacgattta atgtggtggc
    53281 ggcgtctgtt ccgggcgatt gataagtggg caccgccagg acagcgtttg ttattggtga
    53341 ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat gcagatcatt cgtaacttta
    53401 ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa gtatggccgc gtggaactgg
    53461 tcggtccgtt ctccgtgcgt gatggcgaag ataattacca actttatctg attcgtccgg
    53521 ccagcagttc tcaatccgat ttcattaact tactgtttga ccgcccgcta ttactgctga
    53581 ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt ggcctggagt ctggcaaaac
    53641 cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca gggaaactta cgccagcacc
    53701 cggaactgga agcggggcca caggaattcc ttgccgcagg tgccagtttt aaccagatgg
    53761 tcaccgcgct ggagcgcatg atgacctctc agcagcgtct gctttctgat atctctcacg
    53821 agctgcgcac cccgctgacg cgtctgcaac tgggtacggc gttactgcgc cgtcgtagcg
    53881 gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca acgtctggac agcatgatca
    53941 acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc gctggttagc gaaaccatca
    54001 aagccaacca gttgtggagt gaagtgctgg ataacgcggc gttcgaagcc gagcaaatgg
    54061 gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc gctgtacggc aatccaaacg
    54121 ccctggaaag tgcgctggaa aacattgttc gtaatgctct gcgttattcc catacgaaga
    54181 ttgaagtggg ctttgcggta gataaagacg gtatcaccat tacggtggac gacgatggtc
    54241 ctggcgttag cccggaagat cgcgaacaga ttttccgtcc gttctatcgg accgatgaag
    54301 cacgcgatcg tgaatctggc ggtacaggat tggggctggc gattgttgaa accgccattc
    54361 agcagcatcg tggctgggtg aaggcagaag acagcccgct gggcggttta cggctggtga
    54421 tttggttgcc gctgtataag cggagttaaa ctccgcattt gtaggcagga taaggcgttt
    54481 acgccgcatc cggcatttga gcaggatgcc tgatgcgacg ctgatagcgt cttatcaggc
    54541 ctacactccg ggggttattt tccccacaac cggcgggaaa aatcttcgat cttgccgctc
    54601 agtcggcgct tttgcatcgt ccgcgtccag cttttcgata acccggcggc ggagagtaaa
    54661 cggtgatact gatcgtcatc aaacggcata tgccatgcga tggcggcagc ctcctgcacg
    54721 gtcacatcgc tgacacggga aaccaactcc agcggcgcgt ccgcagacac tttccctagc
    54781 gctatcacgc tatacaacca ccccacctta cctgtgtttt gcatcaactg cgctatatcg
    54841 ctgatatcaa aatggtaatt gagtttatag caaggcgagc gcggctggct gacctgaatt
    54901 aatgcctctc cccagcggaa aatatcgccc atatgaacat tactttccgt gagaccgtcg
    54961 gttgagagat tttcgccaaa cgcaggcgca acaaacaact ccgcctgttc cggaaattcc
    55021 cgcacccagt agagatagtg ctcacgagga taatgacaca gcgctctgtc tggcccgccg
    55081 tgaacctttt tctccgcctg ctcgtcacct tccagcccca gctccgtcag catcaactca
    55141 ccatcaacct ggattttggc aattgcgctg ggtttgcctt cgggataagc ctgaatcttg
    55201 cctgtgtata catcaaccgg atatcgcatc tgttccctct ccacgagaaa tttccccatt
    55261 agaccatatt tattttatgt cagccgtgct gttttcgcgg taagtatcca ggcaggaact
    55321 caatagcaaa ccggacgaac gttagttcat ccggctgtgg ggattaagga agcaggttta
    55381 tttcaccgta cctaaaattt cgattttcgc tgctctggct ttgactttac gtgaccagat
    55441 agaggtaaga attggcacca gaatcgaagt cacaatgacc gccgtcgcta ccagcgaagt
    55501 tgctgccgga gccatcggtt taaacgcagg caccatttct gcaatcagca caggtgtcgc
    55561 taccgcggcc cctgcggaac tggaagcggc aattccggca gtgccatcgc caccgccaat
    55621 caatttatcg gcgataatca acggaatacc ggtcacgata attactgcca cacccaacag
    55681 gatccccagc aaaccagtct gggcaatcac agtcaaatca atggtattgc ccagcgcgaa
    55741 ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa aattctcgta attcagggtc
    55801 aagattcccc agggcaaagc ccaccaggaa cggtaatact gcgccgacga aaacatgcgg
    55861 ttcaaacgag gcaatcccgg cagtgcccag aataatcatc gtcatgagcg gcccggactc
    55921 caacgacatc aacacaaatg ccccagcttc ttctttcgta ccgtactgct gcatgatgga
    55981 agcgtaaagt ccgccgttgg tcatatccat cgccgccacc agcgccagcg ttgaaagtcc
    56041 ggcaaagaat ccaacttcaa caccatgttc cggaataatg cgcgaggcaa ttgccgcaac
    56101 cacccacgcg acggcaattt tagttaccac cagcgtaccg gatttacgca gtaccgttcc
    56161 cgtcgcgctt aattttattg acgcccccat gcaaaaaaac cacaccgcca gaatgggcac
    56221 cgtaccggta atcatcccgt tggtgaatga tccaaaatat ttccccgccc ccggcgagaa
    56281 ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc atcccccccg ggattttctc
    56341 aatcgagcgt tttatctgca tttccatcac ctgtcggcat atttttaagg gctgatgcgt
    56401 aattcctcaa tggagcatca gtgttgatgg aaacgaagat agacattcat ttaatcaata
    56461 aaagtgatca aaaccacatc aattgaaacg ctgttttatt tttataggat cattgttttt
    56521 attttggaat tgatcacaaa aaaaccaccg ccgttggcga tggttcattt tttaagctga
    56581 tatgcggcct atacgcctca ttgcagcagg cggcaaatga ttattttttc gccgcaaaac
    56641 gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc tttgatgtag tccggacggc
    56701 ggttctggaa tttcaggtag taagcatgtt cccacacatc caggcccaga atcgggaagc
    56761 cggaagcgcc agaaatagct tcacccatca gcggagaatc ctggttagca gtagaaacca
    56821 ccgccagttt atcgcctttc agcaccagcc atgcccagcc ggaaccaaag cgggaagctg
    56881 ccgctttttc aaattctgct ttgaagttat caacggagcc gaagtcacgt tcgatagccg
    56941 ctttcaggtc accctgcagg gtggtgcctt ttttcagacc tttccagaac aggctgtggt
    57001 tagcgtgacc gccagcgttg ttgcgcagta cggttttctt gtctgctggc agctggtcca
    57061 gtttggtgat cagctcttca accggcaggt tggcaaattc tggcaggctt tccagcgccg
    57121 cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg gtggatttcc atggtctgct
    57181 tatcgaagtg cggttccagg gcatcgtaag catacggcag ggatggcagg gtatagctca
    57241 tattcatctc cagtattgtc gggcggccga ttgttaatgc cgcgtaagca gttggttcat
    57301 tatagttaat taaatgatat tgaaaatgat tatcaatgcc gtacttttcg taagggtatg
    57361 gttttgcagg aaaatgcccg agatgtgaag caaatcaccc acttaatgcc gtgattgcca
    57421 gtaaatcgac aacggcggca acaggcgaaa ggttaatcga cagcacgatt tttacactca
    57481 tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata agaagatgag taacgcgatt
    57541 acgatgggga tattttggca tttgatcggc gcggccagtg cagcctgttt ttacgctccg
    57601 ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt cagtcggtgg gattgtttcg
    57661 tggattattc tgccgtgggc catcagcgcc ctgttactac cggatttctg ggcgtattac
    57721 agctcgttta gtctctctac gctgctgcct gtttttctgt tcggcgctat gtggaggatc
    57781 ggtaatatca actatggcct gaccatgcgt tatctcggca tgtcgatggg aattggcatc
    57841 gccattggca ttacgttgat tgtcggtacg ctgatgacgc caattatcaa cggcaatttc
    57901 gatgtgctga ttaacaccga aggcggacgc atgacgttgc tcggcgttct ggtggcgctg
    57961 attggcgtag ggattgtaac tcgagccggg cagttgaaag agcgcaagat gggcattaaa
    58021 gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg tgatgtgcgg cattttctct
    58081 gccgggatgt cctttgcgat gaacgccgca aaaccgatgc atgaagccgc tgccgcactt
    58141 ggcgtcgatc cactgtatgt cgctctgcca agctatgttg tcatcatggg cggcggcgcg
    58201 atcattaacc tcggtttctg ttttattcgt ctggcaaaag tgaaggattt gtcgctaaaa
    58261 gccgacttct cgctggcaaa accgctgatc attcacaatg tgttactctc ggcactgggc
    58321 gggttgatgt ggtatctgca attctttttc tatgcctagg gccacgcccg cattccggcg
    58381 cagtatgact acatcagttg gatgctgcat atgagtttct atgtattgtg cggcggtatc
    58441 gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc gtccggtaac ggtgttgagc
    58501 ctcggttgtg tggtgattat tgtcgccgcc aacatcgtcg gcatcggcat ggcgaattaa
    58561 tctttctgcg aattgagatg acgccactgg ctgggcgtca tcccggtttc ccgggtaaac
    58621 accaccgaaa aatagttact atcttcaaag ccacattcgg tcgaaatatc actgattaac
    58681 aggcggctat gctggagaag atattgcgca tgacacactc tgacctgccg cagatattga
    58741 ttgatggtca ttccagtctg ctggcgaaat tgctgacgca aaacgcgctc actgcacgat
    58801 gcctcatcac aaaatttatc cagcgcaaag ggacttttca ggctagccgc cagccgggta
    58861 atcagcttat ccagcaacgt ttcgctggat gttggtggca acgaatcact ggtgtaacga
    58921 tggcgattca gcaacatcac caactgcccg aacagcaact cagccatttc gttagcaaac
    58981 ggcacatgct gactactttc atgctcaagc tgaccgataa cctgccgcgc ctgcgccatc
    59041 cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg cgctaaatcc cggaatcgct
    59101 ccctgccagt caagattcag cttcagccgc tccgggcaat aaataatatt ctgcaaaacc
    59161 agatcgttaa cggaagcgta ggagtgttta tcgtcagcat gaatataaaa gagatcgcca
    59221 cgggtaatgc gataaggacg atcgttgagt acatgcagac cattaccgcg ccagacaatc
    59281 accagctcac aaaaatcatg tgtatgttcg gcaaagacat cttgcggata acggtcagcc
    59341 acagcgactg cctgctggtc gctggcaaaa aaatcatctt tgagaagttt taactgatgc
    59401 gccaccgtgg ctacctcggc cagagaacga agttgattat tcgcaatatg gcgtacaaat
    59461 acgttgagaa gattcgcgtt attgcagaaa gccatcccgt ccctggcgaa tatcacgcgg
    59521 tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg ttactgtcgc tgaatccaca
    59581 gcgataggcg atgtcagtga cgctggcctc gctgtggcgt agcagatgtc gggctttcat
    59641 cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc gtttgctgct taagctgccg
    59701 atgtagcgta cgcagtgaaa gagaaaattg atccgccacg gcatcccaat tcacctcatc
    59761 ggcaaaatgg tcctccagcc aggccagaag caagttgaga cgtgatgcgc tgttttccag
    59821 gttctcctgc aaactgcttt tacgcagcaa gagcagtaat tgcataaaca agatctcgcg
    59881 actggcggtc gagggtaaat cattttcccc ttcctgctgt tccatctgtg caaccagctg
    59941 tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga gacggatact gcccatccag
    60001 ctcttgtggc agcaactgat tcagcccggc gagaaactga aatcgatccg gcgagcgata
    60061 cagcacattg gtcagacaca gattatcggt atgttcatac agatgccgat catgatcgcg
    60121 tacgaaacag accgtgccac cggtgatggt atagggctgc ccattaaaca catgaatacc
    60181 cgtgccatgt tcgacaatca caatttcatg aaaatcatga tgatgttcag gaaaatccgc
    60241 ctgcgggagc cggagttcta tcgccacgga cgcgttacaa gacggaaaaa aatccacact
    60301 atgtaatacg gtcatactgg cctcctgatg tcgtcaacac ggcgaaatag taatcacgag
    60361 gtcaggttct taccttaaat tttcgacgga aaaccacgta aaaaacgtcg atttttcaag
    60421 atacagcgtg aattttcagg aaatgcggtg cgcatcacat caccacaatt cagcaaattg
    60481 tgaacatcat cacgttcatc tttccctggt tgccaatggc ccattttcct gtcagtaacg
    60541 agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa tgaaattcag caggatcaca
    60601 ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc atccagtggg cgcgtgatgc
    60661 tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg cgaaatccat cgttttaaca
    60721 atgggctgca tagccaaaac ggttatgtca cctgggatgt ggatagcctg gaaagtgcca
    60781 ttcgccttgg attaaacaag gtgtgcgagg aagggattcg tatcgatagc attgggattg
    60841 atacctgggg cgtggacttt gtgctgctcg accaacaggg tcagcgtgtg ggcctgcccg
    60901 ttgcttatcg cgatagccgc accaatggcc tgatgccgca ggcacaacag caactcggca
    60961 aacgcgatat ttatcagcgt agcggcatcc agtttctacc cttcaatacg ctttatcagt
    61021 tgcgtgcgct gacggagcaa caacctgaac ttattccaca cattgctcac gctctgctga
    61081 tgccggatta cttcagttat cgcctgaccg gcaagatgaa ctgggaatat accaatgcca
    61141 ccaccacaca actggtcaat atcaatagcg acgactggga cgagtcgcta ctggcgtgga
    61201 gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc gggtaatgtc attgggcact
    61261 ggatttgccc acagggtaat gagattccgg tggtcgccgt tgccagccat gataccgcca
    61321 gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc ttatctctct tctggcacct
    61381 ggtcattgat gggcttcgaa agccagacgc catttaccaa tgacacggcg ctggcagcca
    61441 acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt gctgaaaaat attatgggct
    61501 tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa cgatctcccg gcgcttatcg
    61561 ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa tcccaatgac gatcgcttta
    61621 ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg tcgggaaacg gcgcaaccga
    61681 tcccgggaag tgatgctgaa ctggcgcgct gtattttcga cagtctggcg ctgctgtatg
    61741 ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga tttctcgcaa ctgcatattg
    61801 tcggcggcgg ctgccagaac acgctgctca accagctatg cgccgatgcc tgcggtattc
    61861 gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa tatcggcatc cagttaatga
    61921 cgctggatga actcaacaat gtggatgatt tccgtcaggt cgtcagcacc accgcgaatc
    61981 tgaccacctt tacccctaat cctgacagtg aaattgccca ctatgtggcg cagattcact
    62041 ctacacgaca gacaaaggag ctttgcgcat gaccactcaa ctggaacagg cctgggagct
    62101 ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag gaggcgctgc gccaacttga
    62161 tcgtttaccc gtttcaatgc actgctggca gggcgatgat gtttccggtt ttgaaaaccc
    62221 ggaaggttcg ctgaccgggg ggattcaggc cacaggcaat tatccgggca aagcgcgtaa
    62281 tgccagtgag ctacgtgccg atctggaaca ggctatgcgg ctgattccgg ggccgaaacg
    62341 gcttaattta catgccatct atctggaatc agatacgcca gtctcgcgcg accagatcaa
    62401 accagagcac ttcaaaaact gggttgaatg ggcgaaagcc aatcagctcg gtctggattt
    62461 taacccctcc tgcttttcgc atccgctaag cgccgatggc tttacgctct cgcatcccga
    62521 cgacagcatt cgccagttct ggattgatca ctgcaaagcc agccgtcgcg tttcggccta
    62581 ttttggcgag caactcggca caccatcggt gatgaacatc tggatcccgg atggtatgaa
    62641 agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg ctggcagcac tggatgaggt
    62701 gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc gttgagagca aattgtttgg
    62761 cattggcgca gagagctaca cggttggctc caatgagttt tacatggggt atgccaccag
    62821 ccgccagact gcgctgtgcc tggacgccgg gcacttccac ccgactgaag tgatttccga
    62881 caagatttcc gccgccatgc tgtatgtgcc gcagttgctg ctgcacgtca gccgtccggt
    62941 tcgctgggac agcgatcacg tagtgctgct ggatgatgaa acccaggcaa ttgccagtga
    63001 gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc cttgacttct tcgatgcctc
    63061 tatcaaccgc attgccgcgt gggtcattgg tacacgcaat atgaaaaaag ccctgctgcg
    63121 tgcgttgctg gaacctaccg ctgagctgcg caagctggaa gcggcgggcg attacactgc
    63181 gcgtctggca ctgctggaag agcagaaatc gttgccgtgg caggcggtct gggaaatgta
    63241 ttgccaacgt cacgatacgc cagcaggtag cgaatggctg gagagcgtgc gggcttatga
    63301 gaaagcgatt ttgagccagc gtgggtaaac actgccggat gcggcacgag cgccttatcc
    63361 ggcctacggg tcgaatgtcc atgcgggctg tcaccctcac cccggccctc tccctgaaag
    63421 ggcgagggag aaaaacatag cctcgtgcgg caacggagct gaacaccgct ccggacagtc
    63481 ccctcgcccc tacggggaga gggttagggt gaggggaaca agccgcacca tgtctaaaca
    63541 aggcaaccta atttacagga acacagaaca tgcaaaacat tacccagtcc tggtttgtcc
    63601 agggaatgat caaagccacc accgatgcct ggctgaaagg ctgggacgag cgcaacggcg
    63661 gcaacctgac gctgcgcctg gatgacgccg atatcgcgct ttatcacgac aacttccacc
    63721 cacaaccgcg ctatattccg ctcagccaac ccatgccttt actggcaaat acgccgttta
    63781 ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct cgatcctgca gctaacttag
    63841 gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat tctctggggg ttaaccaacg
    63901 aagccgttcc aacttctgaa cttccggctc actttctctc ccactgcgag cgcattaaag
    63961 ccaccaacgg taaagatcgg gtgatcatgc actgccacgc caccaacctg atcgccctca
    64021 cctatgtact ggaaaacgac accgcggtct ttactcgcca actgtgggaa ggcagcaccg
    64081 aatgtctggt agtattcccg gatggcgttg gcattctgcc gtggatggtg cccggcacgg
    64141 acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca ttcgctggtg ttgtggccct
    64201 tccacggtgt gttcggttcc ggaccgacgc tggatgaagc ctttggttta atcgacaccg
    64261 cagaaaaatc agcacaagta ttagtgaagg tttattcgat gggcggcatg aaacagacca
    64321 tcagccgtga agagttgata gcgctcggca agcgcttcgc cgttacacca ctagccagtg
    64381 cgctggcgct gtaaggagca aacatgatcc gcaaagcctt tgtcatgcag gtaaaccccg
    64441 acgcccacga agagtatcag cgtcggcata atcccatctg gccggaactg gaagcagtgt
    64501 tgaaatctca cggtgtgcat aactacgcca tttacctcga caaagcgcgt aatctgctgt
    64561 ttgccatggt agagattgaa tctgaagaac ggtggaatgc ggtagccagt actgatgttt
    64621 gccaacgttg gtggaaatat atgaccgatg ttatgcccgc taacccagat aacagcccgg
    64681 tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt gcccaatgtg ccggagatgc
    64741 ctccggcaca atattttaaa tttcgatttg aaaaattaaa catattacat ctcccggtaa
    64801 gttatatttc cctgatacat tgtgagtaaa tcacaaaaat aatgaataac ccattaatga
    64861 ttcatgtggt ttatttaaat aaccattatg tgcattactc cgcaaatctg acctttcact
    64921 ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atc
//
LOCUS       DADLSE010000005        63890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLSE010000005 DADLSE010000000
VERSION     DADLSE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.005"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..2565
                     /locus_tag="JN692_000317"
     CDS             <1..2565
                     /locus_tag="JN692_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HAY7262726.1"
                     /translation="SGGVPSFIGGLVVFVSAAFNAQAETWFDPAFFKDDPSMVADLSR
                     FEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAF
                     PAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGI
                     NALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEW
                     KNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVV
                     RGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYS
                     VPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGPWGTTWYGGGQYA
                     EYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQ
                     LMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQ
                     QLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQV
                     FALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLS
                     YSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQG
                     ITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASL
                     KRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTN
                     SGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2556..3626
                     /locus_tag="JN692_000318"
     CDS             2556..3626
                     /locus_tag="JN692_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262727.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLEARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3638..4180
                     /locus_tag="JN692_000319"
     CDS             3638..4180
                     /locus_tag="JN692_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262728.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4188..4703
                     /locus_tag="JN692_000320"
     CDS             4188..4703
                     /locus_tag="JN692_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7262729.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4669..5406
                     /locus_tag="JN692_000321"
     CDS             4669..5406
                     /locus_tag="JN692_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7262730.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5517..6527
                     /gene="pyrD"
                     /locus_tag="JN692_000322"
     CDS             5517..6527
                     /gene="pyrD"
                     /locus_tag="JN692_000322"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7262731.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6701..7243
                     /gene="zapC"
                     /locus_tag="JN692_000323"
     CDS             6701..7243
                     /gene="zapC"
                     /locus_tag="JN692_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7262732.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN692_000324"
     CDS             complement(7240..8349)
                     /gene="ycbX"
                     /locus_tag="JN692_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7262733.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN692_000325"
     CDS             8593..10701
                     /gene="rlmKL"
                     /locus_tag="JN692_000325"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7262734.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWAVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10713..12620
                     /locus_tag="JN692_000326"
                     /pseudo
     CDS             10713..12620
                     /locus_tag="JN692_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12750..14003
                     /gene="pqiA"
                     /locus_tag="JN692_000327"
     CDS             12750..14003
                     /gene="pqiA"
                     /locus_tag="JN692_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7262735.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLMTILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGIGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14008..15648
                     /gene="pqiB"
                     /locus_tag="JN692_000328"
     CDS             14008..15648
                     /gene="pqiB"
                     /locus_tag="JN692_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7262736.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15645..16208
                     /gene="pqiC"
                     /locus_tag="JN692_000329"
     CDS             15645..16208
                     /gene="pqiC"
                     /locus_tag="JN692_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7262737.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16464..16631
                     /gene="rmf"
                     /locus_tag="JN692_000330"
     CDS             16464..16631
                     /gene="rmf"
                     /locus_tag="JN692_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7262738.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN692_000331"
     CDS             complement(16701..17219)
                     /gene="fabA"
                     /locus_tag="JN692_000331"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7262739.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17288..19048)
                     /locus_tag="JN692_000332"
     CDS             complement(17288..19048)
                     /locus_tag="JN692_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7262740.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19233..19685
                     /gene="matP"
                     /locus_tag="JN692_000333"
     CDS             19233..19685
                     /gene="matP"
                     /locus_tag="JN692_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7262741.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN692_000334"
     CDS             complement(19761..20807)
                     /gene="ompA"
                     /locus_tag="JN692_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7262742.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPSGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN692_000335"
     CDS             complement(21163..21672)
                     /gene="sulA"
                     /locus_tag="JN692_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7262743.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21891..22520
                     /gene="sxy"
                     /locus_tag="JN692_000336"
     CDS             21891..22520
                     /gene="sxy"
                     /locus_tag="JN692_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7262744.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN692_000337"
     CDS             complement(22483..24645)
                     /gene="yccS"
                     /locus_tag="JN692_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7262745.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24655..25101)
                     /locus_tag="JN692_000338"
     CDS             complement(24655..25101)
                     /locus_tag="JN692_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7262746.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25224..27278
                     /gene="helD"
                     /locus_tag="JN692_000339"
     CDS             25224..27278
                     /gene="helD"
                     /locus_tag="JN692_000339"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7262747.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN692_000340"
     CDS             complement(27310..27768)
                     /gene="mgsA"
                     /locus_tag="JN692_000340"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7262748.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(27864..28526)
                     /locus_tag="JN692_000341"
     CDS             complement(27864..28526)
                     /locus_tag="JN692_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7262749.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28699..29112
                     /locus_tag="JN692_000342"
     CDS             28699..29112
                     /locus_tag="JN692_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7262750.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN692_000343"
     CDS             complement(29157..29474)
                     /gene="hspQ"
                     /locus_tag="JN692_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7262751.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN692_000344"
     CDS             complement(29532..30722)
                     /gene="rlmI"
                     /locus_tag="JN692_000344"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7262752.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            30817..31095
                     /gene="yccX"
                     /locus_tag="JN692_000345"
     CDS             30817..31095
                     /gene="yccX"
                     /locus_tag="JN692_000345"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7262753.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN692_000346"
     CDS             complement(31092..31421)
                     /gene="tusE"
                     /locus_tag="JN692_000346"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7262754.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN692_000347"
     CDS             complement(31512..32171)
                     /gene="yccA"
                     /locus_tag="JN692_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7262755.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32378..32465)
                     /locus_tag="JN692_000348"
     tRNA            complement(32378..32465)
                     /locus_tag="JN692_000348"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32429..32431),aa:Ser,seq:tga)
     gene            32891..34009
                     /gene="hyaA"
                     /locus_tag="JN692_000349"
     CDS             32891..34009
                     /gene="hyaA"
                     /locus_tag="JN692_000349"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7262756.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34006..35799
                     /gene="hyaB"
                     /locus_tag="JN692_000350"
     CDS             34006..35799
                     /gene="hyaB"
                     /locus_tag="JN692_000350"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HAY7262757.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALVIVQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            35818..36525
                     /gene="hyaC"
                     /locus_tag="JN692_000351"
     CDS             35818..36525
                     /gene="hyaC"
                     /locus_tag="JN692_000351"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7262758.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36522..37109
                     /locus_tag="JN692_000352"
     CDS             36522..37109
                     /locus_tag="JN692_000352"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7262759.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37106..37504
                     /gene="hyaE"
                     /locus_tag="JN692_000353"
     CDS             37106..37504
                     /gene="hyaE"
                     /locus_tag="JN692_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7262760.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37501..38358
                     /locus_tag="JN692_000354"
     CDS             37501..38358
                     /locus_tag="JN692_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7262761.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38492..40036
                     /gene="appC"
                     /locus_tag="JN692_000355"
     CDS             38492..40036
                     /gene="appC"
                     /locus_tag="JN692_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7262762.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIVLVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40048..41184
                     /gene="appB"
                     /locus_tag="JN692_000356"
     CDS             40048..41184
                     /gene="appB"
                     /locus_tag="JN692_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7262763.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVASNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFSAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41196..41288
                     /gene="cbdX"
                     /locus_tag="JN692_000357"
     CDS             41196..41288
                     /gene="cbdX"
                     /locus_tag="JN692_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7262764.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            41368..42666
                     /locus_tag="JN692_000358"
     CDS             41368..42666
                     /locus_tag="JN692_000358"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7262765.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN692_000359"
                     /pseudo
     CDS             complement(42781..44947)
                     /gene="etk"
                     /locus_tag="JN692_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN692_000360"
                     /gene_synonym="yccY"
     CDS             complement(44967..45413)
                     /gene="etp"
                     /locus_tag="JN692_000360"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7262766.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(45401..46540)
                     /locus_tag="JN692_000361"
     CDS             complement(45401..46540)
                     /locus_tag="JN692_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7262767.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(46586..48682)
                     /locus_tag="JN692_000362"
     CDS             complement(46586..48682)
                     /locus_tag="JN692_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HAY7262768.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSIGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(48682..49428)
                     /locus_tag="JN692_000363"
     CDS             complement(48682..49428)
                     /locus_tag="JN692_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7262769.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(49425..50069)
                     /locus_tag="JN692_000364"
     CDS             complement(49425..50069)
                     /locus_tag="JN692_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7262770.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(50176..50481)
                     /locus_tag="JN692_000365"
     CDS             complement(50176..50481)
                     /locus_tag="JN692_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262771.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN692_000366"
     CDS             complement(50923..51135)
                     /gene="cspH"
                     /locus_tag="JN692_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HAY7262772.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            51421..51633
                     /gene="cspG"
                     /locus_tag="JN692_000367"
     CDS             51421..51633
                     /gene="cspG"
                     /locus_tag="JN692_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HAY7262773.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            51644..51832
                     /locus_tag="JN692_000368"
     CDS             51644..51832
                     /locus_tag="JN692_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAY7262774.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            51807..52037
                     /gene="ymcE"
                     /locus_tag="JN692_000369"
     CDS             51807..52037
                     /gene="ymcE"
                     /locus_tag="JN692_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HAY7262775.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNIPYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            52027..52200
                     /locus_tag="JN692_000370"
     CDS             52027..52200
                     /locus_tag="JN692_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HAY7262776.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(52249..53322)
                     /locus_tag="JN692_000371"
     CDS             complement(52249..53322)
                     /locus_tag="JN692_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HAY7262777.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN692_000372"
                     /pseudo
     CDS             complement(53424..56090)
                     /gene="torS"
                     /locus_tag="JN692_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309175.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            56218..57246
                     /gene="torT"
                     /locus_tag="JN692_000373"
     CDS             56218..57246
                     /gene="torT"
                     /locus_tag="JN692_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HAY7262778.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGNPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN692_000374"
     CDS             complement(57219..57911)
                     /gene="torR"
                     /locus_tag="JN692_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HAY7262779.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            58041..59213
                     /gene="torC"
                     /locus_tag="JN692_000375"
     CDS             58041..59213
                     /gene="torC"
                     /locus_tag="JN692_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HAY7262780.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            59213..61759
                     /gene="torA"
                     /locus_tag="JN692_000376"
     CDS             59213..61759
                     /gene="torA"
                     /locus_tag="JN692_000376"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HAY7262781.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            61756..62355
                     /gene="torD"
                     /locus_tag="JN692_000377"
     CDS             61756..62355
                     /gene="torD"
                     /locus_tag="JN692_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HAY7262782.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIATLTVRDDARLELAADFCGLFLMTDKQVALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAALCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN692_000378"
     CDS             complement(62448..62753)
                     /gene="cbpM"
                     /locus_tag="JN692_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7262783.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN692_000379"
     CDS             complement(62753..63673)
                     /gene="cbpA"
                     /locus_tag="JN692_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7262784.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGAPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        15720 a        15825 c        16822 g        15523 t
ORIGIN      
        1 tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat
       61 gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc
      121 gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt
      181 gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag
      241 aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat
      301 gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt
      361 ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa
      421 atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc
      481 aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac
      541 agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg
      601 agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat
      661 ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat
      721 gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca
      781 gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc
      841 aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta
      901 tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta
      961 gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg
     1021 ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc
     1081 aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg
     1141 tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt
     1201 ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt tcgatgcgac ccaggcgaag
     1261 agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa
     1321 acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt
     1381 ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt
     1441 gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc
     1501 aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt
     1561 agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac
     1621 acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa
     1681 cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc
     1741 agaagtaatg atagctatac caggaaaaaa aattacgcct ggatgacctc taacaccagt
     1801 atcgataacg aagggcacat tacacaaaac ctgggtttaa cggagacact actcgatgac
     1861 ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac ggctaatggt
     1921 agcgccagca tggactacaa aggggcgttt gcagatgccc gagtgggcta caactacagc
     1981 gataacggca gtcaacaaca actgaactac gctctttcag gcagtttagt tgcccattca
     2041 cagggtatta ccctggggca atcgctgggg gaaactaacg tgctgattgc agcaccaggc
     2101 gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg cggatatacc
     2161 gttgtcccct atgccacttc ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta
     2221 aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg
     2281 gttctggcgg agttcaatgc ccatgcggga gcaagggtat taatgaaaac atcaaagcag
     2341 ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcatacagac taatagcggc
     2401 attattgatg atgatggctc gctctatatg tccggtttgc ccgctcaggg agcgataact
     2461 gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca gttaccagct taccgaacaa
     2521 caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt ggagaaaaat
     2581 gcgtgttatt actacgacta ttttttgccg ccgtcttaat gctatggtgc gctcaaactg
     2641 ctgcttatat cgggcagtgt cataccactc aggggaatcc gtatattggc gtcaattttg
     2701 gcgttaaaac tctggaggaa gaagaaaata cggctggagt agttaaagac aaattttatc
     2761 agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac aatgtcagaa
     2821 gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac aactggtaca
     2881 aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt tctcctactg
     2941 cggttccttt cgaaaacgtg ggcacagggg cagatacacg atggcatatt tgcgatcccg
     3001 gcggtcaacg tttaggtggc cagggggcta gcggtaatag cggtagcttt tccctgaaaa
     3061 tattgcagcc gttcgttggt tcggtcgtca ttcctcctat ggcgctggcg cgattatttg
     3121 aatgctacaa catacccgca ggtgattcct gcacgactac tggtacaccg gttttagtgt
     3181 attacctgtc tggtacgatc aattcacttg gctcatgttc cgtcaatgcc ggagaaacaa
     3241 tcgaggttga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg cataagcctc
     3301 ttgaggccag aacggcagaa cttgcaattc cggtcaggtg taacacggga aacgcagggt
     3361 tagttaacgt caatctgagt ctgacggcaa cctcagaccc cagctatccc caggcgatta
     3421 agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac attatttccc
     3481 ctgctggtgg gacactaccg ctctctattc ctgatgatgc agacagtatc gcgcgaatga
     3541 atgtctatcc agtcagcacg acaggcgtac caccagaaac cgggcgattt gaagccacgg
     3601 caacggtgag aataaatttt gattaacgcg tgaacgtatg aagaaaaaaa cgatatatca
     3661 gtgcgttatt ttgttcttta gccttcttaa catccatgtc gggatggctg ggcctgaaca
     3721 agtcagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag
     3781 tgcattacaa aatattcata ttggtgattt taatatcagt gattttcagg ccgcgaatac
     3841 cgtaagcact gctgctgatt tgaatattga tatcaccggt tgtgccgctg gtattactgg
     3901 cgcggacgtc ctttttagcg gcgaggctga cacccttgcg ccgacactgc tcaaactaac
     3961 tgacacaggc ggaagcggtg gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca
     4021 aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa aagccgggga
     4081 taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa
     4141 tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg ttgaaacgga
     4201 taatctggat tctgttctta ctgggattaa cggggggctg tgagctattt gcccatgatg
     4261 gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca caggatagca
     4321 agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg tttttttcat gggaattatg
     4381 gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc agcacggttg
     4441 acgtcacctt ttcaggaacc cccgacggag tgcagagcga aatgctgtcc attgaaagtg
     4501 gtacggatgc agccagtggg ctggcaattg cgattctgga tgatgcgaag atattgattc
     4561 cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca ttaacttttt
     4621 atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat gccagcgcga
     4681 catttgtact tcattatgac taatacgtgg aacagattag cgctcttgat tttcgccgtt
     4741 ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg aacgcgattt
     4801 atctttccgg cagacagaga atcgatatct attttactga ctaataccag tcaggaatcc
     4861 tggcttatta acagcaaaat caatcgccca acgcgttggg ccggggggga agcgtcgaca
     4921 gtgccagcac tattactggc cgctccgcca cttattctcc tgaagcccgg tactacaggc
     4981 acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt
     5041 gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg
     5101 atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa
     5161 gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg
     5221 ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag taatgcggga
     5281 gtcgtgccgc ctaaaagcca gcgtcagaca aactggtgtc aggtaatagc accgtgtcat
     5341 gtcgcctggc ggacgataaa tgattacggc gggttgagtg caaagaagga gcaaaatctg
     5401 ccctgaaaca ggttctgaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg
     5461 tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt
     5521 actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta
     5581 cttttcagca attacgccgc attaccggaa caccgtttga agcactggtg cggcagaaag
     5641 tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag
     5701 ccggtcttga taaagacggg gagtgcattg atgcgttagg cgcgatggga tttggatcga
     5761 tcgagatcgg taccgtcacg ccacgtccac agcctggtaa tgacaagccg cgtctctttc
     5821 gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata
     5881 acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt cttgggtatt aatatcggca
     5941 aaaataaaga tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa
     6001 tctatgccta tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac
     6061 gcacgctgca atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa
     6121 atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc
     6181 tttctgaaga cgaattgatc caggttgccg atagtttagt tcgccataat attgatggcg
     6241 ttattgcaac caataccact ctcgatcgtt ctctggttca gggaatgaaa aattgcgatc
     6301 aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag cacagaaatt attcgccgct
     6361 tgtcactaga attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta
     6421 tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta
     6481 tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa
     6541 ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta tattcatcgt
     6601 tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg
     6661 gattgtgttg ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga
     6721 taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg
     6781 tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc
     6841 aggcttttgc gttgatgatg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt
     6901 taatttatct aaagaccaaa aagccgaact ggtgctgaat gcactggtag caattcgtta
     6961 tctcaaaccg caaatgccga aaagctggca ttttgtctcc catggtgaaa tgtgggtgcc
     7021 catgccgggc gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct
     7081 ggtcgttgaa tctggcgaaa atgccgcgct atgcctgctg gcacaaccct gcgttgttat
     7141 tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc
     7201 gcaggtgaat gttgacagct tcagcctcga acaggcagtc taacgcgtca gcttaagggc
     7261 agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt
     7321 cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg
     7381 gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca cttgttggtt
     7441 atttccacga aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt
     7501 gatgttggcg gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag
     7561 aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc
     7621 aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc
     7681 gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca
     7741 aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccagc tatcttcttc
     7801 ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc
     7861 tggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc
     7921 atcagcaaat gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc
     7981 cacccagcgt aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc
     8041 tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc
     8101 gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc
     8161 atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt
     8221 accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag
     8281 agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa
     8341 tgtcgccacg gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct
     8401 tattagctat aatgcgcaac aattttctta gcgcctgtcc cgttaggcta ttttatttgt
     8461 cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc
     8521 gcgctctcca tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt
     8581 aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta
     8641 aaaactgaac tggaaaatct gggggccgtt gaatgccagg tggttcaggg tggggtccat
     8701 ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgctt ggcctcgcgt
     8761 attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt
     8821 caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt
     8881 ggtttgaatg acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc
     8941 gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat
     9001 atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt
     9061 gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc
     9121 ctggcagccg ctattgtgat gcgatccggc tggcagtcag gaacaccgct gctcgatccg
     9181 atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcacca
     9241 ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg
     9301 caggaagtga aagcggaagc gcaaactcgc gcccgtaaag gcctggctga gtatagctct
     9361 catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt
     9421 cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat
     9481 ccgctgccga aagggccgta cggtacagtg ttgagcaacc cgccatacgg tgaacgtctg
     9541 gacagcgaac cggcgctgat tgcgctgcat agtctgctgg gccgaatcat gaaaaaccag
     9601 ttcggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcctgcag
     9661 ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat
     9721 taccacgttg ccgaaagcac accagacagt aaaccggcaa tggtagcgga agattacgcc
     9781 aaccgcctgc gtaagaacct caaaaaattc gagaagtggg cacgccagga agggattgaa
     9841 tgttaccgcc tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttatgcc
     9901 gactgggcgg tggtgcagga gtatgcgccg ccaaaaacta ttgatgcgca caaagcgcgt
     9961 cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa
    10021 ctggtgctga aaacccgtga acggcagaag ggcaaaaacc agtaccagaa actgggcgag
    10081 aagggcgagt ttcttgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat
    10141 tatctcgata ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct cggtcagatg
    10201 agcaaaggta aagatttcct caacctgttt tcttataccg gcagcgccac cgtgcatgcg
    10261 ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta tctggaatgg
    10321 gcagaacgca acctgcgtct gaatggcctg accaggcgtg cgcatcgcct gattcaggcc
    10381 gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt tatcgatccg
    10441 ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg
    10501 gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac
    10561 aacaaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa
    10621 gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc
    10681 tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat
    10741 ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg
    10801 aacgtgtttg tctggtgggc cgcaacggcg caggcaaatc gacgttaatg aaaatcctca
    10861 accgtgaaca agggctggat gacggtcgca ttatttatga gcaagatttg attgtagctc
    10921 gtctgcaaca ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag
    10981 gcattgaaga acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga
    11041 acgacccgag cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ctggatcacc
    11101 acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc
    11161 ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg
    11221 cgctggtgag caatccgcgc gtattgctgc tggacgaacc gactaaccat ctggatatcg
    11281 aaaccatcga ctggctggag gggttcctta aaaccttcaa tgggacgatt attttcatct
    11341 cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca
    11401 agctggtgac ctatccaggg aattacgacc agtacctgca ggaaaaagaa gaagccctgc
    11461 gcgtggaaga actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaagtgtgga
    11521 ttcgctaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag
    11581 cgatgcgtcg cgaacgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg
    11641 aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggtta
    11701 acggtaagca actggtgaaa gatttttctg cccaggttct acgtggcgac aaaattgccc
    11761 tgattggtcc gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc
    11821 aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc
    11881 accgcgcgga actggatccc gataaaacgg tgatggataa ccttgccgaa ggtaagcaag
    11941 aggtgatggt taatggcaag ccacgccacg tattgggcta tttgcaggac tttctgttcc
    12001 atccgaaacg ggcgatgacg ccagtacgtg cgctttctgg cggtgagcgg aaccgcttgc
    12061 tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg
    12121 atcttgatgt cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg
    12181 tattgctggt tagccacgat cgtcagtttg tcgataacac cgttacagaa tgctggatct
    12241 tcgaaggcgg cggtaaaatt ggtcgttatg ttggcggtta tcatgatgcc cgtggtcagc
    12301 aagaacaata tgtggcactc aaacagcctg cggtgaaaaa aagcgaagaa gccgccgcgc
    12361 caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac
    12421 tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc
    12481 aagtggcgga tgcttccttc ttcagtcagc cgcatgagca gacgcaaaaa gtgcttgctg
    12541 atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag
    12601 cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg
    12661 taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct
    12721 gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca
    12781 tcctgtgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag
    12841 cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc
    12901 ccaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg
    12961 tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc
    13021 ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc
    13081 ccgcgttttg tctgatgacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt
    13141 taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt
    13201 tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc attgggatag
    13261 gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg
    13321 ttgatcgtcg ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa
    13381 aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc
    13441 ttcctgctga tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa
    13501 acagcctgca gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta
    13561 atattttgcc catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg
    13621 ctggggtcat tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg
    13681 ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca
    13741 aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg
    13801 taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta
    13861 tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca
    13921 taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg
    13981 aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag
    14041 aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc
    14101 tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat
    14161 gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg
    14221 gttgaaagcg ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat
    14281 tccggtatgg aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt
    14341 ggtcgcgaag ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag
    14401 ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcacc accgttggcc
    14461 ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg
    14521 ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc
    14581 gatacgcaaa aacgcaatat cagttatcaa ctgttcatca atgcacctta tgaccgactg
    14641 gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagcg
    14701 gggatgcgtg tggagatggg ctcattgaca acgctgctta gtggcggtgt tagctttgat
    14761 gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat
    14821 gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc
    14881 tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc
    14941 ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat
    15001 gattaccgta ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc
    15061 gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg
    15121 ctgaaaaccg gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat
    15181 acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc gactgttagc
    15241 ggcggcctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg
    15301 ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg
    15361 aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca gtcgatgcag
    15421 cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc
    15481 cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg
    15541 ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt
    15601 gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag
    15661 tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt
    15721 accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt
    15781 tatgggtaga gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa
    15841 ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac
    15901 agttgcgcaa caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct
    15961 cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag tttaacggtc
    16021 gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga
    16081 tcaaacgtcc gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta
    16141 aagtgctggc cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc
    16201 taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgttgcct gaggcggttt
    16261 ttttgtttct aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg
    16321 cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat
    16381 tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga
    16441 aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac
    16501 atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga
    16561 cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag
    16621 taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt
    16681 gatactctgt ctgatgataa tcagaaggca gacgtatcct ggaacagacc gactttcagg
    16741 tcgctggcgg tatagatcag acgacaatca accagcactt cgccatctgc cagacccata
    16801 atcagacgac gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc
    16861 ggcagtacct gaccagtgaa tttcacttcg ccaacgccca acgcgcggcc tttaccttcg
    16921 ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag gcccaggcat
    16981 cccggcataa ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata
    17041 tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc
    17101 acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt
    17161 tcaccgtgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg
    17221 ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca
    17281 agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac
    17341 gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt
    17401 ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta
    17461 ctgcccagat ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta
    17521 aatggcgaac gttagcagtg gggataatga caccttgttt ccccgttaac tcacgttgct
    17581 ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc tggacgcggc
    17641 cgaactgatc gactgaacct gtgatagcga tactctgatt cactggcaca tcagcgaggg
    17701 cgcttatcag ggcgcagagt tcagccatcg aggcactatc gccatccact tcactgtatg
    17761 actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca
    17821 tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca agctccgctt
    17881 tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag
    17941 gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc
    18001 cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac
    18061 gttcagcgag gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc
    18121 cggagaaggt gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact
    18181 gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta
    18241 ccggccaagc atccgctccc ggggcaggta agtgattatg tctggcggta aatgtcaccc
    18301 agcgacacca ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc
    18361 tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc
    18421 gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag acggggagag
    18481 ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc
    18541 gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat
    18601 ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc
    18661 caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat
    18721 tatcatctgc actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgat
    18781 cgcccaccag ttggcctgca tcgctttgta gcgtacgcgc ggcatcggca ataagattca
    18841 gatactcaga ctcttccggg gccttagcca gcataaaaga ggaggatgct cgcgtatgca
    18901 gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa ggatcgtttt
    18961 cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt
    19021 cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg
    19081 cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcttggctg
    19141 ggcgttaaag ctcacaggat ggagattctt ttcttcactg gcctaaaaag ctgatattct
    19201 gtaaagagtt acacggtaac attgagatcg ctatgaaata tcaacaactt gaaaatcttg
    19261 aaagcggttg gaaatggaag tatctggtga agaagcatcg cgaaggggag ttaattaccc
    19321 gttacataga agccagtgcc gcccaggaag ccgtcgatgt gttgctctcg ctcgaaaatg
    19381 aacccgtgct ggtaaatggc tggattgaca agcatatgaa tccggaactg gtcaatcgca
    19441 tgaagcagac cattcgggcc agacggaagc gccattttaa tgcggaacac cagcatacgc
    19501 gcaaaaaatc gatcgatctg gaatttattg tctggcaacg tctggctggt cttgcacagc
    19561 gtcgcggtaa aacgctttct gaaacgattg ttcagctgat tgaagatgcg gaaaacaaag
    19621 agaaatacgc gaataaaatg tcttctctga agcaggatct gcaggcattg ctgggtaagg
    19681 aataactgac gaaagtcagt tcaatttact aaaggcaaaa aaaaccccgc agcagcgggg
    19741 tttttctacc agacgataac ttaagcctgc ggctgagtta caacgtcttt gataccttta
    19801 acttcgatct ctacgcgacg atccggagcc aggcagtcga tcagtgcagc acgctgtttc
    19861 acgttgtcac aggtgttgcc agtaaccggg ttggattcgc ccataccacg tgcggagatc
    19921 ttgtctgccg ggataccttt ggagatcagg taatcaacaa cagactgagc acggcgctcg
    19981 gacagaccct ggttgtaagc gtcagaaccg atgcggtcag tgtaacccag aacaactacg
    20041 gaaccgtctt tcggatccag gttgctcagc tggctgtaca gctgatccag agcagcctga
    20101 ccttccggtt tcagggttgc tttgttgaag ttgaacagaa cgtcagactt cagagtgaag
    20161 tgcttggtct gtacttccgg tgccggagct ggagcaacta ccggagctgc ttcgccctga
    20221 ccgaaacggt aggaaacacc caggctcagc agaccgttgt ccggacgagt accgatggtg
    20281 tttgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    20341 atcgcgtact caacaccacc tgcgaaaacc ggagaaacgc cagtgtcgtg gtctttaaag
    20401 gatgcgccac taggtacgtt agccttggtg tctgcacgcc ataccatacc acccagacga
    20461 gtgtagatgt ccagatcgtc agtgattggg taacccagtt tagcggtcag ctgaacgccc
    20521 tgagctttgt atgcgccgtt gatgttgtcg cctttgtacg gcatacgacc taaccagtcg
    20581 taacccattt caaaaccaac atacgggtta acctggtaac caccaaaagc acctgcaccc
    20641 agttggtttt cgtgggtcgg accattgtta ggaataaaac cagtgtcatg gtactgggac
    20701 cagcccagtt tagcaccagt gtaccaggtg ttatctttcg gagcggcctg cgctacggta
    20761 gcgaaaccag ccagtgccac tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg
    20821 ttatcatcca aaatacgcca tgaatatctc caacgagata acacggttaa atccttcacc
    20881 gggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctagc
    20941 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    21001 aaaaaagtct tgtataaggt atgtttaatc tttttgtcag cgacaattta cagaaaaaaa
    21061 acgcggaaac cgcttcagac aagccttcgc aaggaaaatt agtcacgact gaaagcattg
    21121 gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat acaaattaga
    21181 gtgaattttt agcccggaaa gttgtctcgt tgtgtgagag gatgcgctta ccggacgcat
    21241 aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat gctcttcttc
    21301 agtcaaatca tctgtcaacc aaccgatcac cacactgtaa ttgcccgtgc gtaaagcgcg
    21361 aaccattgac tccacagtgt ggcaagggga gagctggcta atctgcatta ctttcgttaa
    21421 gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg ttaaccagag
    21481 ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa gttgcgtcat
    21541 catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag tgttttccgt
    21601 agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat gtgcatagcc
    21661 tgaagtgtac ataatcaatc cagcccctgt gagttactgt atggatgtgc agtacatcca
    21721 gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt cgttggtgac
    21781 gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg
    21841 atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt atgaaaagcc
    21901 tctcctataa gcggatctat aaatcacaag aatacctggc aacgttgggc acaattgaat
    21961 accgatcatt gtttggcagt tacagcctga ccgttgacga cacggtgttt gcgatggttt
    22021 ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt gtaaaacatc
    22081 cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat tactatcggg
    22141 ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa tattctctcg
    22201 atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa gatttgccca
    22261 atatgtcttt tcatctggaa gcgattctcg gggaggtggg gattaaggat gtacgggcgt
    22321 tacgtatact tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac agtctggtga
    22381 cagaaaagat tctgtttatg cttgaaggtg ccattatcgg cattcatgaa gctgcgctcc
    22441 cggtggcacg ccgccaggag cttgcagaat gggctgactc tcttacgccg aaacaggagt
    22501 ttcctgcgga gcttgagtaa tctggcgttg cagacgacca atctctggca gtaatgcaat
    22561 caataaccca acttgttgta cgaccagagg ttctttgcta tctgcccgtg gttcaagttg
    22621 ctgcatccgc tgtttcaggc cagctaatgc ctgattgacg cgttcttcat cagcaggttg
    22681 atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca gaatttcagg
    22741 attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa acgtatggtt
    22801 aaggcacagc aagcgaaacg cggcttcgcg aatttgcggg gtaacgttcg gttcgctgga
    22861 catatttgat accaccgacg ccagttcagc atcacggttg tgtgcatcgc ggcgggcaat
    22921 acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg catcgagata
    22981 gcgacagttg gcctctgtgg cgcgttcgag cattcgcggc agattgcgaa acttccagtc
    23041 aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg tatcgattac
    23101 gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta gcaccaaaag
    23161 tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa agagcacgcc
    23221 ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca gcacaggaat
    23281 gccaatcgcg atacctacca gcgtaccaat aatccttaac ttcaggcggt ggcgcgtggc
    23341 gttatagttt ggctggcaga cgaacaaact tgtcagcaag atccaatacc cgtgatgcat
    23401 tccggttatt tgaatgatgg cgtagccgaa gcacaacacc agcgacattc ttaccgcatg
    23461 acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga tatcactcaa
    23521 tccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttacggggta gtgcctgggc
    23581 ctgttctgat tcaattgttg ccagttgggc atcaatggca cgtaaattgt tcagcaaaaa
    23641 tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct ccagcgcagc
    23701 atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataagact gacgcaacaa
    23761 aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc gctgaaaacg
    23821 gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa tatgagaaga
    23881 gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc gacgcgttcc
    23941 ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct gattcaatgt
    24001 cgccatcagc tgaccgttgg cgagagccaa atcgtacagc ggtgcctggc tttcatcttc
    24061 aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt gttcatagca
    24121 acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa taagtgtcag
    24181 gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc agtgctcata
    24241 cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg caattgttgc
    24301 atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg ttaagccaat
    24361 cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga taaaaaagca
    24421 gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga gatcggtcag
    24481 cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac ccagccacca
    24541 cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca gccaggcgct
    24601 gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag tggattattc
    24661 aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg ataccacgcg
    24721 acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc cgacaggaat
    24781 gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc ataaccacca
    24841 gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca gcaacacatt
    24901 tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag gcaccagaga
    24961 cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca gcacaatact
    25021 gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca caaagttcag
    25081 aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta atgggcggca
    25141 attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg ataagaaata
    25201 atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga aaacgtctgg
    25261 cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc tccggcgatc
    25321 gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag cgcggtctgg
    25381 tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg cacggcacag
    25441 aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg cggtggagtg
    25501 gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg attgccacgc
    25561 gcactggaga aaataaatgg ctgacgcgtg agcaaacctc tggcgttcag caacaaatcc
    25621 gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc gataactgcc
    25681 gtgaggcgtg gcgtaaatgt caggcttggt tgaaagatat tgaaagcgct cggttgcagc
    25741 ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc cgccaggtcg
    25801 agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag cattctctgt
    25861 tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt
    25921 tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt ggtcgcaaag
    25981 ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac attaccgcac
    26041 gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag
    26101 tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct gagtggcgca
    26161 agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg acggaagaaa
    26221 tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag cgtcgccttg
    26281 cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca caggcagaaa
    26341 tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc aagttgatgg
    26401 ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg
    26461 gccttattca tcaggcgatt gtgattctgg agaaaggccg ctttatcagc ccgtggaagc
    26521 atattctggt tgatgaattt caggatatct cgccgcagcg cgcagcgttg ttagcggcat
    26581 tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg caggcgattt
    26641 accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag
    26701 gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt gaggtggcaa
    26761 accggtttat tcagcagaac ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg
    26821 gagacaaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg ctggataagc
    26881 tctctggtta tgccaaaccg gaagagcgca ttctgatcct ggcgcgttac catcacatga
    26941 ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc gactttatga
    27001 ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc ttgcaggagg
    27061 ggagtgatgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg ctactgccac
    27121 cggttgagga tttcccggac gctgaagaac ggcgtttaat gtacgtggcg ctgacccggg
    27181 cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt gtggaaatac
    27241 tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc
    27301 tgtgcaatat tacttcagac ggtcagcgag ataacgctga taatcgggga taagaatatc
    27361 gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct gccgttgcca cgttggtggc
    27421 gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg
    27481 cacggcattt agtggatccc agaagaaaat caatacatca attttccctt ctgagatcaa
    27541 tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga cgttcatgcc
    27601 ggtcgcgcgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt gttgttccag
    27661 taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat cgtgtgccac
    27721 cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa tgtacatccg
    27781 tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa gaccgaaaaa
    27841 gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca ttgcaggaaa
    27901 gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg ttctgtgagc
    27961 acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag cgtactgtca
    28021 tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt
    28081 tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag aatatccagc
    28141 tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa
    28201 tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg gttgatgagc
    28261 tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc actattggaa
    28321 agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattgtc cagttcaatg
    28381 ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac cagaagatcg
    28441 acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca taatgcaatc
    28501 aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc ccgaataata
    28561 atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt cccgcttttg
    28621 ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta tactttgtta
    28681 aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac gtccactcac
    28741 accattgcgc tggtgggggc aagcgacaaa cccgatcgcc ccagctatcg cgtgatgaaa
    28801 tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc cggcaaaacg
    28861 ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaggt cgatatggtc
    28921 gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat tgccattggc
    28981 gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt actggcacgg
    29041 gatgccgggt taaatgtggt gatggatcgc tgcccggcta ttgagatccc tcgcctgggt
    29101 ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatttgtt cagggattag
    29161 ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc atccatggat
    29221 ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc caggtaggta
    29281 tgaaccggta ggccgttatc gtcctccatc accacgtgat accacggagc ggcgcgaagc
    29341 tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgaataaac cgggtcgata
    29401 tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc gataccgaat
    29461 ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag ggtaaaattc
    29521 cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc ttccgggtag
    29581 gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg tacatcacga
    29641 ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt catcagaccg
    29701 gaacaggaga aagtcaggag aataccgcct tcattcagca actgaatcgc cagcatgttg
    29761 atgtctttat aaccacgaca cgcgcccatc aactggcttt tattctcaac aaacttcggc
    29821 gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg cagcaattta
    29881 aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag ctcaacgttc
    29941 tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg
    30001 cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag cacacgttta
    30061 ttttcaacgt agcggcgggt agccaggcgg ctgtctcgct ggtccagata gtagcccgtt
    30121 ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt gttcttcaat cggcagcagg
    30181 gcaggcggca actcgccggt gacgaggccc tgggtcagct ccatcccttc ttttttacgt
    30241 accgcaacat cgctgcgatc gtaaatcgca cattccgggt acagcgtttg cagggcacta
    30301 attaatgccg cgcgctgata ttctgcgcca gcactcagca gttgcagcac cagaaaatta
    30361 ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat taaacgatag
    30421 ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg
    30481 cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac gcgcgcccgg
    30541 atttgcgaag ctggcgaata agcgccgcgt gctaaccatt ttccctgatg atcaacaata
    30601 tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc ggaaaagacc
    30661 cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa acgtacactc
    30721 ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga
    30781 tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg cataattgcc
    30841 tggatttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta cgaagcgaaa
    30901 agactgggct taaccgggta cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc
    30961 tgcggtgaag aagggcaggt agaaaaatta atgcagtggt taaagagtgg cggcccgcgt
    31021 tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga attaaccgat
    31081 tttcgtatcc gttaaataca ttttaccggt tttggcaggc cggcaatttt ggtggcttgc
    31141 ttagccgggc ctttcgggaa cagtcggtat aaatagcggc tgttaccttt ttcttcacca
    31201 aatttattcg ccatcgcttt taccagcata cgaatcgccg gagacgtatt aaattccagg
    31261 tagaagtcac gtacaaaacg caccacttcc cagtgttctg gcgacagcga aatcccttcg
    31321 ttctctgcaa tcaccactgc cagcggttca ctccactggc tgctttcttt gagatagcct
    31381 tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac taccttaatc
    31441 agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt
    31501 agcgggtgag attaatcgcg gctggcgaag cccagaatgc tcagcaggct gacgaagatg
    31561 ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtttc accgccatga
    31621 atgatgttgc tggtttcaaa caagatagcg ccagaggaga tcagaatgaa gaccgcgctg
    31681 atcgccagat gcagagcagg cagttgcagg aagatattag caaccatacc aatcagtacc
    31741 accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg ggtggtcagc
    31801 acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat agcgattacg
    31861 tcacccattc cggcagacag ataggtgttc agaattggtc cgaggatata acccagaaaa
    31921 ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt ataggtcagg
    31981 aacatcaaac catacatacc caccagcgtc agaatcagac ccggagatgg cagcatcagc
    32041 accgtactgg cagttgcggt aatcgccgaa aaggccagcg tcaggctcag cagaaaatag
    32101 gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga agaactaaca
    32161 atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc ataatgataa
    32221 tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga acaggtttaa
    32281 cagcggatta tcaggtcatt aagcaaatat aacgccctga gaattccgac aggcaaaaga
    32341 aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca gagattcgaa
    32401 ctctggaacc ctttcgggtc gccggttttc aagaccggtg ccttcaaccg ctcggccaca
    32461 cttccggaat gacgcgcact ataaacatcc cgatgcggcg tgtaaacccc taatttgttt
    32521 gtttgcctga aaaatagcca aaagtgcatt gatagcgtga aataacagca gattgatcat
    32581 ttcatcacca tgaattcctt ctcttttact cgtttagcaa ccggctaaac atccccatcg
    32641 cccgggcaaa gaaaaatagg tccattttta tcgctaaaag ataaatccac acagtttgta
    32701 ttgttttgtg caaaagtttc actacgcttt attaacaata ctttctggcg acgtgtgcca
    32761 gtgcagaagg atgagctttc gttttcagca tctcacgtga agcgatggtt tgccttgcta
    32821 cagggacgtc gcttgccgac cataagcgcc cggtgtcctg ccggtgttgc taggagaaga
    32881 gacgtgcgat atgaataacg aggaaacatt ttaccaggcc atgcggcgtc agggcgttac
    32941 ccggcgcagc tttctcaaat attgtagtct ggctgccacg tcgctgggat taggcgcggg
    33001 aatggcacca aagattgcct gggcgctgga gaacaaaccg cgcattccgg tggtatggat
    33061 ccacggtctg gaatgtacct gctgtaccga atcttttatc cgctccgctc acccactggc
    33121 gaaggacgtc atcctttccc tgatttccct cgattacgac gataccctga tggctgccgc
    33181 cggaacccag gcggaagaag tctttgaaga catcatcacg caatataatg gcaaatatat
    33241 cctcgcagta gaaggtaatc cgccgctggg cgagcagggg atgttctgta tcagcagcgg
    33301 tcgaccgttt attgagaaac tcaaacgtgc cgctgccgga gccagcgcga ttatcgcctg
    33361 gggaacctgc gcgtcctggg gctgcgtgca ggccgcgcga cctaatccga cacaggcaac
    33421 gcctatcgac aaagtcatca ccgacaaacc cattatcaaa gtacctggct gcccgccgat
    33481 cccggatgtg atgagcgcca tcattactta catggtgacc tttgatcgct tgccagatgt
    33541 cgacagaatg ggtcgtccac tgatgtttta tggtcagcgt atccacgata aatgctatcg
    33601 ccgcgctcac ttcgacgccg gagagttcgt ccagagttgg gatgatgacg ctgcccgcaa
    33661 aggttattgc ctgtacaaaa tgggctgcaa agggcctacc acctataacg cctgttcctc
    33721 cacacgctgg aatgatggcg tttctttccc aatccagtct ggtcacggct gcctgggctg
    33781 tgcggaaaat ggtttctggg atcgcggttc gttctacagc cgcgtggtcg atattccgca
    33841 aatgggtact cattccaccg ccgataccgt cggtttaacc gcgcttggcg tggtggcagc
    33901 agctgttggt gtgcacgcag ttgccagcgc cgttgaccaa cgcagacgtc ataaccagca
    33961 acctacagaa accgaacatc agccaggcaa tgaggataaa caggcatgag cactcagtac
    34021 gaaactcagg gatacaccat caataatgcc ggacgccgcc tggtggtcga cccgattacg
    34081 cgcatcgaag gccacatgcg ctgcgaagtg aatattaacg atcagaatgt gatcaccaat
    34141 gccgtctcct gcggcaccat gtttcgcggg ctggagatca tcctgcaagg gcgcgacccg
    34201 cgcgatgcgt gggcgttcgt tgaacgtatc tgcggcgtct gtactggcgt acacgccctg
    34261 gcttcggttt acgccatcga agatgccatc ggtattaaag tgccggacaa cgccaatatc
    34321 atccgcaaca ttatgctggc aacgctctgg tgccacgatc atctggtgca cttctatcag
    34381 cttgccggga tggactggat cgacgtgtta gatgcgctga aagccgaccc gcgaaaaacc
    34441 tccgaactgg cgcaaagtct ctcctcttgg ccgaaatcat cccctggcta tttcttcgac
    34501 gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt tggggatctt ccgcaatggc
    34561 tactgggggc acccgcagta caaactgccg ccagaagcca acctgatggg ctttgcccac
    34621 tatctcgaag ctctcgattt ccaacgtgaa attgtcaaaa ttcacgcggt ctttggcggt
    34681 aaaaacccgc atccaaactg gattgtcggc gggatgcctt gcgccattaa cattgacgaa
    34741 agcggcgcgg tcggggcggt caatatggaa cgcctgaacc tggtgcagtc gattatcacc
    34801 cgcacggcag acttcattaa caacgtgatg atccccgacg ccttagtcat cgttcagttc
    34861 aacaaaccgt ggagcgaaat cggcacgggt ctttctgata aatgtgtcct cagctacggc
    34921 gcattcccgg atattgccaa cgactttggt gagaaaagtc tgctgatgcc tggcggcgcg
    34981 gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc gcagcaggtg
    35041 caggagtttg tcgatcacgc ctggtatcgt tatcccaacg atcaggtcgg gcgtcatcca
    35101 ttcgatggca ttaccgaccc gtggtacaac cccggcgatg tcaaaggcag cgataccaac
    35161 attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg ctggcgcggt
    35221 aacgcgatgg aagtggggcc gctggcacgc acgttaatcg cttatcacaa aggcgatgct
    35281 gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct ttccggtatc
    35341 cagtcaacgt tgggccgcat tttgtgccgc gcgcacgaag cgcagtgggc cgcaggtaag
    35401 ttgcagtatt tcttcgacaa gctgatgacc aacctgaaaa acggcaatct cgccactgct
    35461 tccactgaaa aatgggaacc tgcaacctgg ccgacagagt gccgtggcgt cggctttacc
    35521 gaagcgccgc gcggagcgtt aggccactgg gccgccattc gcgatggcaa gattgatctc
    35581 taccagtgcg tggtgccgac cacctggaac gccagcccgc gcgatcccaa agggcagatt
    35641 ggcgcttatg aagcggcgct gatgaacacc aaaatggcga tccccgagca accgctggag
    35701 atcctgcgta ctctgcacag ctttgatccg tgcctcgcct gttcaactca tgtgctgggc
    35761 gacgacggta gcgagctgat ctccgtgcag gtgcgttaac agcgaaggag aatcatcatg
    35821 caacagaaaa gcgacaacgt tgtcagccac tatgtctttg aagcaccagt tcgtatctgg
    35881 cactggctga cggtgttatg catggcggtg ttgatggtca ccggttactt tatcggcaag
    35941 ccgctacctt ccgtcagtgg cgaggcgacg tatctgttct atatgggtta catcaggtta
    36001 attcacttca gcgccgggat gatttttacc gtggtcttgc tgatgcggat ctactgggct
    36061 tttgttggca atcgttactc ccgcgagctg tttatcgtgc cggtatggcg taaaagctgg
    36121 tggcagggcg tgtggtatga aatccgctgg tatctgtttc tggcaaaacg tccgagtgcc
    36181 gatataggcc ataaccccat cgcccaggcg gcgatgttcg gctatttcct gatgtcggtc
    36241 tttatgatca tcaccggttt tgcgctgtac agcgaacaca gccagtacgc tatttttgcg
    36301 ccgttccgtt atgtggtgga atttttctac tggacgggtg gcaactcaat ggacattcac
    36361 agctggcatc ggctggggat gtggctgatt ggggcgtttg tgatcggtca tgtctacatg
    36421 gcgctgcgtg aagacatcat gtccgacgac acggtgatct ccaccatggt caacggctac
    36481 cgtagccaca aatttggcaa aataagtaac aaggagcgtt catgagcgag caacgcgtgg
    36541 tggtcatggg gctgggcaac ctgctgtggg ccgatgaagg cttcggcgtg cgggtggcgg
    36601 aacggctgta tgcccattac cactggcccg agtatgtgga gattgtcgat ggcggtactc
    36661 agggactgaa cttgctgggg tatgtcgaaa gcgccagcca tctgttgatt ctcgatgcca
    36721 ttgactacgg gctggaacct ggaacgctgc gaacctatgc cggagaacgc attccggcct
    36781 atctcagcgc gaagaaaatg agcctgcatc agaacagttt ctccgaagtg ctggcgctgg
    36841 cagatatccg cggacatctg ccagcacata ttgccctcgt cgggctgcaa cccgcgatgc
    36901 tcgacgacta cggcggcagc ctgagcgaac tggcacggga gcaactgccc gctgcggaac
    36961 aatcggcgct ggcgcagttg gctgcgtggg gaagtgtgcc gcaaccggct aatgaatcgc
    37021 gttgtctcaa ttatgactgt ctgtcgatgg aaaattacga aggcgttcgc ttgcgccagt
    37081 accggatgac acaggaggag cagggatgag caacgacacg ccatttgatg cgttgtggca
    37141 acgaatgctg gcgcgcggct ggacgccggt cagtgaatcc cgtcttgacg actggcttac
    37201 gcaagcgcca gacggcgtgg tgttattaag cagtgacccg aaacgcacgc cagaggtcag
    37261 cgataatccg gtaatgattg gcgaattact gcacgagttt cccgactata catggcaggt
    37321 ggcgattgct gaccttgagc agagcgaagc catcggcgat cgctttggcg cctttcgctt
    37381 tcctgccact ttagtgttta ccggcggaaa ctatcgcggc gtgctgaatg gtattcaccc
    37441 gtgggcggag ctgataaacc tgatgcgcgg gcttgtcgaa ccgcagcagg agcgtgcctc
    37501 atgagcgaaa cttttttcca tctgctgggg ccaggaacgc aaccgaacga tgacagtttc
    37561 agcatgaatc cactgccgat cacctgtcag gtgaatgatg aaccgagtat ggcggccttg
    37621 gagcaatgtg ctcacagccc gcaggtgatt gcgctgttaa acgagttaca acatcaacta
    37681 agcgaacgcc agccgccgtt gggcgaggtg ctggcagtcg atctgttaaa tctcaacgcc
    37741 gacgatcgtc actttatcaa tacgcttctc ggggaagggg aagtgtcagt gcgcattcag
    37801 caggctgacg acagtaaaag tgaaatacag gaggcgatct tctgcggatt atggagggtg
    37861 cgcagacgtc gcggcgaaaa gttgctggag gacaaactgg aggctggctg cgcgccgctg
    37921 gcgttgtggc aggcggcaac gcaaaatctc ttgccgacag attcgctgtt accgccgccc
    37981 attgatggcc tgatgaatgg cctaccgttg gcgcatgagt tactggcaca tgtacgtaac
    38041 cccgacgcgc agccgcacag cattaatctg acgcaattac ccatcagcga ggctgatcgg
    38101 ctttttctct cacgtctctg tgggccggga aatattcaga ttcgtaccat tggctatggc
    38161 gagagctata tcaacgccac ggggttacgc catgtctggc atttacgctg tacggacacc
    38221 ttaaaaggcc cgttactgga aagttatgaa atatgcccaa taccggaagt ggtgctggca
    38281 gcgccagaag atttggtcga ctctgcgcag cggcttagcg aggtatgtca gtggctggcg
    38341 gaagctgcac cgacgtaaaa agacggtaag tatcgctttc agtcttatga atatcgcaat
    38401 cggcgaatac ctctggtcgt agagtttcag gataaagagg gagatctaac attatcgggt
    38461 tatttttctc tcttcgccta caggagtgcg catgtgggat gtcattgatt tatcgcgctg
    38521 gcagtttgct ctgaccgcgc tgtatcactt tttatttgta ccccttacct tggggctgat
    38581 ttttttgctg gctatcatgg aaaccattta cgtggtcacc ggcaaaacaa tctaccgcga
    38641 tatgacgcgc ttctggggta agctcttcgg tatcaatttt gctcttggcg tggctaccgg
    38701 cctgaccatg gagtttcagt ttggtactaa ctggtcattc tattccaact atgtgggcga
    38761 tatttttggc gcaccactgg cgatggaagc attaatggcc ttcttcctcg aatccacctt
    38821 tgtcgggctg ttcttcttcg gctggcaacg gctgaataaa taccagcacc tgctggtgac
    38881 gtggctggtg gcgttcggtt caaatctctc tgcattgtgg atattgaatg ccaacggttg
    38941 gatgcaatac ccgaccggtg cgcattttga tatcgacacc ctgcgtatgg agatgaccag
    39001 cttcagcgag ctggtcttta acccggtcag ccaggtgaaa tttgtgcaca ccgtcatggc
    39061 gggctacgtg accggggcca tgtttattat ggcgatcagc gcctggtatt tactgcgcgg
    39121 acgggagcgc gatgtcgcat tacgctcgtt tgccatcggt tccgtcttcg gtactctggc
    39181 gattatcggt accctgcaac tcggagacag ttctgcgtat gaagtcgcgc aagtacaacc
    39241 ggtaaaactg gcggcgatgg aaggggagtg gcaaacggaa cctgctcctg caccgttcca
    39301 tgtggttgcc tggccggaac aggatcaaga gcgtaacgcc tttgccatca aaattcccgc
    39361 gctgctaggg atcctcgcca ctcactcatt agataaaccc gtgccgggtc tgaagaattt
    39421 gatggctgaa acctacccac gcttgcaacg cggacgtatg gcctggctgt taatgcagga
    39481 aatatctcaa ggcaatcgtg agccgcatgt gttgcaggca ttccgggagc tggaaggcga
    39541 ccttggttac ggcatgttgc tctcccgcta tgcgccggat atgaatcatg tcacagccgc
    39601 acagtaccag gcggcgatgc gtggcgcgat acctcaggtt gcgccggtat tctggagttt
    39661 ccgcatcatg gtgggctgtg gttccctgct gctactggtg atgctgattg tgcttgtcca
    39721 gacgctgcgt ggcaaaatcg accagcatcg ctgggtgctg aaaatggcgc tctggagttt
    39781 gccgttgccg tggattgcga ttgaagccgg gtggtttatg accgagtttg gtcgtcagcc
    39841 gtgggcgata caggacatct taccgacata ctccgcgcac tccgctttaa ccacaggaca
    39901 actggctttc tcactgatca tgatcgtagg gctttacacc ctgttcttaa tcgccgaagt
    39961 ctacctgatg cagaaatatg cccgtctggg gccgagcgcg atgcagagtg aacaaccgac
    40021 gcagcaacag gggtaaagga gaaaatcatg tttgattatg aaacattgcg cttcatctgg
    40081 tggctgctga ttggcgtgat cctggtggtc tttatgatct ccgacggatt tgacatgggg
    40141 atcggctgtt tgctgccgct ggtggcgagt aatgatgatg aacgccggat agtgattaat
    40201 agcgttggtg cgcactggga aggcaatcag gtctggttga tcctcgcagg tggggcatta
    40261 tttgccgcct ggcccagagt gtatgcagcg gcgttttccg gcttttatgt ggcgatgatc
    40321 ctggtgctgt gctcgctgtt cttccgtccg ctggcctttg attatcgcgg aaaaattgcc
    40381 gatgcacgct ggcgtaaaat gtgggatgcc ggtctggtca tcggcagtct ggtgccgccg
    40441 gtagtcttcg gtatcgcctt cggcaacttg ttgctcggcg tgccgtttgc cttcacaccg
    40501 caattacgcg tggagtatct cggcagcttc tggcaactgc tgacgccatt ccctttattg
    40561 tgtggtttgc tcagccttgg gatggtgatt ttgcaaggtg gcgtctggtt acaactgaaa
    40621 actgttggtg tgattcatct gcgttcacag ctggcgacta aacgcgctgc actgttggtg
    40681 atgctgtgct ttttgctggc aggttactgg ctgtgggtcg gtattgatgg ctttgtactg
    40741 ctcgcccagg atgctaacgg tccttccaat ccgttaatga aactggtggc agtgctacct
    40801 ggtgcctgga tgaataattt cgtcgaatcg cccgttttgt ggatcttccc gctgctgggg
    40861 ttcttctgcc cattgctgac ggtgatggcg atttatcgtg gtcgcccggg ttggggattt
    40921 ttgatggcat cattgatgca atttggcgtg attttctcgg caggcatcac gctgttcccc
    40981 tttgtcatgc cgtcaagtgt gagtccaatc tccagcctga cgttgtggga cagcacttcc
    41041 agtcagctga cgctgagcat tatgttggta atcgtgctga tatttttgcc cattgtgttg
    41101 ctctacactc tctggagcta ctacaaaatg tggggacgca tgacaacaga aactctccgc
    41161 cgtaacgaaa acgagttgta ctaaggagca ggaacatgtg gtatttactt tggttcgtcg
    41221 gcattttgtt gatgtgttcg ctctccaccc ttgtgttggt atggctggac ccgcgtctga
    41281 aaagttaacg aacgtaggcc tgatgcggcg cattagcatc gcatcaggca atcaataatg
    41341 ttagatatga aaagcggaaa catatcgatg aaagcgatct taatcccatt tttatctctt
    41401 ctgattccgt taaccccgca atctgcattc gctcagagtg agccggagct gaagctggaa
    41461 agtgtggtga ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg
    41521 caggatgtca ccccagacgc atggccaacc tggccggtaa aactgggttg gctgacaccg
    41581 cgcggtggtg agctaatcgc ctatctcgga cattaccaac gccagcgtct ggtagccgac
    41641 ggattgctgg cgaaaaaggg ctgcccgcag tctgctcagg tcgcgattat tgctgatgtc
    41701 gacgagcgta cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca
    41761 ataaccgtac atacccaggc agatacgtcc agtcccgatc cgttatttaa tcctctaaaa
    41821 actggcgttt gccaactgga taacgcgaac gtgactgacg cgatcctctg cagggcagga
    41881 gggtcaattg ctgactttac cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg
    41941 cttaatttcc cgcaatcaaa cttgtgcctt aaccgtgaga aacaggacga aagctgttca
    42001 ttaacgcagg cattaccatc ggaactcaag gtgagcgccg acaatgtctc attaaccggt
    42061 gcggtaagcc tcgcatcaat gctgacggag atatttctcc tgcaacaggc acaaggaatg
    42121 ccggagccgg ggtggggaag gatcaccgat tcacaccagt ggaacacctt gctaagtttg
    42181 cataacgcgc aattttattt actacaacgc acgccggagg ttgcccgcag tcgcgccacc
    42241 ccgttattgg atttgatcat ggcagcgttg acgccccatc caccgcaaaa acaggcgtat
    42301 ggtgtgacat tacccacttc agtgctgttt attgccggac acgatactaa tctggcaaat
    42361 ctcggcggcg cactggagct caactggacg cttccaggtc agccggataa cacgccgcca
    42421 ggtggtgaac tggtgtttga acgctggcgt cggctaagcg ataacagcca gtggattcag
    42481 gtttcgctgg tcttccagac tttacagcag atgcgtgata aaacgccgct gtcattaaat
    42541 acgccgcccg gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc
    42601 atgtgttcgt tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt
    42661 ttgtaatgca taaaaaagag cattcagtta cctgaatgct ctgaggctga tgacaaacga
    42721 agaactgtct aatgcgtaga ccggaaaagg cgttcacgcc gcatccggtc actttcagtt
    42781 ttactctttc tcggagtaac tataaccata atagttatag ccgtaactgt aagcggtgct
    42841 ggcgcgttta atcacaccat tgaggattgc gcctttaata ttaacgccag cctgttccag
    42901 acgctgcatt gacaagctca cctctttggc ggtgttcaag ccaaaacgcg caaccagcag
    42961 gctggtgccg acagagcgtc ccacgaccgc agcatcgctc accgccagca tcggcggcgt
    43021 atcgacaatc accagatcgt aatggtcgtt cgcccattcc agtaattgac gcatccgatc
    43081 gcgcatcagc agttcagacg ggttaggtgg cacctgaccg cgagtaatca catcaaagcc
    43141 tcctttgcca aaatgctgga tcactttgtt gagctcatct ttacctgcca gatattccga
    43201 caagccatgt tcattactca cggtaaacag gttatgcgaa taaccacggc gtaagtcggc
    43261 atcaataaat aacacttttt gatcggactg ggcgatcacc gctgccagag ttgaactgac
    43321 aaacgtttta ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat tctcagtctc
    43381 catcatagcg aaatgcagac tggttcgtag cgcacgtacg gcttccacag cagaatccgc
    43441 cgggttatcc accgccagga aggggatatt tttagtacga tggcgctgct gattagaaaa
    43501 taaatttttc ttacgcagac gggtgcgttt atccagccac tcggacattg ggatagtgga
    43561 ataaacgctg atgccgtgct cttccagttg ttccggggct tctacaccac gacgcaacat
    43621 cgcacgcgcc agcacggcac tcaccgaaat aaacaggcca agaataaaac caagcaccac
    43681 attcaacgct tttttcggtt tcactggctg cggctgagtg actgccgggt cgataatccg
    43741 cacgttacca atggcactgg atttcgaaat actcaactcc tgctggcggt taagtaattg
    43801 cagatatacc gcacggcccg cttctacgtc acgacttaaa cgcaacactt cctgttgggt
    43861 ggaaggcatt gccgataccc gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg
    43921 tttttccagc agcgcacgat aagttgggtg atctttctta tacagcaggg agatctctgc
    43981 ctcgcggaag gtcagctcat tgagttgatt atcaacgttc acaatctgct caagaacggc
    44041 tttggcttcc aggttaaggt caaccgaatc gcgctgctgg cgataaacgt tgagtttttc
    44101 ttccgcctgg tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga attcaaggct
    44161 ttgtgaatcc tgcgccgcct ggcgagcgat attctgttgc aaatagttgt tagcgatgct
    44221 gttcagaata cgagtaatca actggggatc atcaccagtc atggtaagtt ccagcatccc
    44281 gctttcttta ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag
    44341 ttcggtacgc tggctcagga caaactgtgt tcctggtttg gccttaatgt ccgcgatagt
    44401 cagcgcaacg ccatcttttt ccagacgctg gccgaccata ccattgacgg tgaactcttc
    44461 accttccagt gtatagtggc cgttttcccc aaccgtgagt gtcagttgct gatcctgacc
    44521 attcagttgt ggaatatgca tccagctgat cgccagctca cctggttttt ctttggttaa
    44581 tctcgcccag ccgcgaccca caatcggaaa atacttctgc tcaactatgt cgcgcagatt
    44641 cagttcagca atggttttac cgagaatcat gcgcgattgc agcagttgga tctccggtgc
    44701 agactcgggc gatgagttag ggatcatatc gctcaggccg ctgagaatgg cgttgccctg
    44761 tttttgctca acctggacca gagtatctgc ctgataaatt ggtgtgctta acagcgatca
    44821 gcgtgaataa cgcggtcacg ctgataataa acttacggtg atcccataac tcgccgacca
    44881 gacgaagcag atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag
    44941 ttgtcatcgt attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg
    45001 gcgcgctcca acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc
    45061 gggatctctt tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga
    45121 gcaattgcgg taacctgagc aatatgttcc gactccattg ccagaatcag atcgtaattt
    45181 cgtgccatct cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta
    45241 gcggcaacat ctgccgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc
    45301 gatttgactt ttacgcccgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg
    45361 caaatgttgc cggtacaaac cactaggatt gagttaaatt ttagttgggc catgtttgaa
    45421 tccaccgtga agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa
    45481 tcacacggtt ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa
    45541 gctggaattc cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat
    45601 tggcgatttt gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat
    45661 cagcgacatc ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc
    45721 tgcggtccat tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat
    45781 catcgttgcg cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt
    45841 cgccacgctg catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca
    45901 ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga
    45961 tggtcagagg aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt
    46021 acgctttttg cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg
    46081 aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag tgttttcccg gccactttca
    46141 ggcgaccaat ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac
    46201 tggcgctacg gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca
    46261 tcaacacatc accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg
    46321 ggttagcctg cgacataatc ggtttggcgc gcagctgatc aatcaatcct ggcgtgacgg
    46381 gatagatatt gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct
    46441 tgttactggt cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag
    46501 ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca
    46561 atgtaatgtt atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca
    46621 ttggatcgag ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc
    46681 acgaaaccac cgcgcggttg cggttaggtc caatggtcat caagtcgaac ggaatcgaga
    46741 tataaaaacc tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg
    46801 ccgcccatac ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgcgc
    46861 ctttatcttt tgccagatat tgcccaatgc taagtttcat cagtacgcca ttgagcgtcg
    46921 gcgggttcca gtaagccgtc acgaaacctg tgggcgtgga ataatcggtg aagcgcatca
    46981 tgttgtccca gtcacgctgc ttcacgtagt taacgtccac acccagcgcc cagctggcat
    47041 ctagcgggcg ataaagaagc tcggaaccga caccggcata catcgtttcc agataaccgc
    47101 catacacctg gccatagaaa ccattgccta agtcggcaaa gtaattcgct tgcaagttgt
    47161 tgagataaac gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag
    47221 agtccgcggg taacagtgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa
    47281 taccgccatc aagcagcagg tggtcggtaa accagtagcg ggcgctggac atcagcccca
    47341 gctggaacat atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag
    47401 agtagctaaa gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg
    47461 cttcaacacg ttgttgttgc agttgctctg atttaccagg cgctgtgccg gccagctgtt
    47521 tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg
    47581 tcacgctaat cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat
    47641 cgaccgcttc gcgagagtca cggtatttgt attgctgacc agacatatac agcgtcttat
    47701 cgcgtagctg aatttctggc gcgtcaaagc ccgcgttata cttcagggcg gtaagttggt
    47761 ttgctaccgt ggtgtactgc aaaccttcag actctggcgc aggctgccag gccggttttg
    47821 gcgtatcgcg tagggcaggg cgcagatcgt tgaaattggt ccgtaaagtg aaaccaaaca
    47881 tcaacgtgtt tccgcgttca taactgaggt tgaggtctgc ccagctggca gcacgataaa
    47941 ctgcaccgac gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat
    48001 tgttgccgtc gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc
    48061 cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt
    48121 gagactctgc gcgatgacaa tatttatcgc tcacccggca aaacgggttg gtaatattgc
    48181 ccgcattacc ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct
    48241 tgctggccac cagatattca ccgtcaaaca aacccgtacc agcaatatca cgtttaccaa
    48301 acgccacttg cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt
    48361 tgtctttgta tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca
    48421 catccgtata acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga
    48481 accggtattg atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg
    48541 catttggcat ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat
    48601 ccgggtaggt taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta
    48661 aaagataaga attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg
    48721 gtcatttaaa tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg
    48781 gctgccaggc ggtggttcca catgtctttt attccacagg gcaacagggg cgacgacagt
    48841 ctcaccctca ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg
    48901 atcttgcaga taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cagccccaga
    48961 aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc
    49021 gaccaacggg ggattgctgt tctcatccac tcgtacaaaa tcagggtcca gtttgaccgg
    49081 taagcgacca gtgatattga gattgagtaa ctgctggcgc acggatttaa tcgttgccgc
    49141 cacatcgtcg tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc
    49201 tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg
    49261 atcgcgcagt tgcggttgtg tcaccaactg gacaacattt tccacggggc caacggaaag
    49321 tgtctgttgt tcgctaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt
    49381 cattacgtaa agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta
    49441 agaaacgtca tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc
    49501 tggccagtag ccgaatcgac ccagaatgtg ttttgccact gtttttctgg cagcgtggaa
    49561 gtcacctctt cctgccagac attacacagc acattgtgac ccgcaatatt cagcgtctct
    49621 aatccagcaa aggaaaaagt agaacttagg gtcgcagagc gaaaatggtt gtcttcactc
    49681 cagagaatat cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg
    49741 tcccgcccgg gatttgtcac ttccagcaga ttattattaa gtttgacggt ctttaaaaga
    49801 cgtccattgt gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt
    49861 tctatatatc ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg
    49921 gaataaggca acgcctggat ctgctgatcc gctacagtga tatcctgatt atcgaaaagg
    49981 ctggcgcgaa atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt
    50041 gcgaaaatcg ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat
    50101 gcaaatctct aaagcccggt acgaatatcg caaccgggta ttatttaact tgaatttcga
    50161 cgcgtttaag cagaattagc gcgtagtgga cgtggtggtt gaggtgttat gagaaccgcc
    50221 atcgccactg tcatttgctg caactactgc caacgtcgcc acaccaacag ccgttgccac
    50281 accgacaact gtcgccgtgc cgacagcgcc agcagccacg ccactagtgg tagccgcagt
    50341 cgtggttgca cctgtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc
    50401 gtttgctgct tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat
    50461 tgcagaaagc ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct
    50521 tgagatcagg attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac
    50581 cgctagcact atatactgcg ggtgggaaag gcgcgcagag ggaaatataa gattgttaac
    50641 gatttccctc tggaaatacg acttgtgagc ataagataaa tcttagaaaa atacgaatta
    50701 aagataagaa aattgttacg aaagctatta attattgttg gtaatatagt ttcaagtggt
    50761 actataactg gctattaaat ggcatttata actattaggt gcagagatat tcgcttaaat
    50821 ggattagttt cttggtggaa aagtaactga tgttataatg tatggtgttg actattgaac
    50881 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
    50941 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacgcgta atcctggtat
    51001 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctggactt ctttacgacc
    51061 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
    51121 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
    51181 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
    51241 caattatcat gccttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
    51301 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
    51361 taagtggttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
    51421 atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt
    51481 atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat
    51541 gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt
    51601 ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca
    51661 tttccgctgt ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga
    51721 gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa
    51781 cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc
    51841 tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa
    51901 gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt ccttacctga
    51961 ccagggtacc gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg
    52021 ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc
    52081 gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc
    52141 atcgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
    52201 ttttgtgaac atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat
    52261 cattggcgct tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac
    52321 tcccgcatac attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat
    52381 taaccagcca gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt
    52441 caatgttgag gctaccggac atgactctac gcaagtcata cagccggtac attccactgt
    52501 tcgtacctga attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc
    52561 acaatcgata caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac
    52621 gcccatcagc gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct
    52681 ggcaatcagc aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc
    52741 gagcatttta acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg
    52801 agcgggcatc aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg
    52861 cagaggaata tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag
    52921 atcgccgatt aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt
    52981 gagtaataga ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga
    53041 cggtaactga cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca
    53101 gccttcgata cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg
    53161 cacccaatac cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt
    53221 cgctttacgc caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt
    53281 gcctggccgc cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta
    53341 agttgcatac caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc
    53401 gcgttcaggt ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct
    53461 tcgtgtggaa ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg
    53521 ctacgcattc ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc
    53581 tcgctgtcct ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt
    53641 ttaaataatg ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg
    53701 agttgcgaga catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt
    53761 aactgaccga gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg
    53821 gtacgctggc gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac
    53881 ggatattgct gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa
    53941 tccaccagtg cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca
    54001 ttgccaatag caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg
    54061 gttagcgggt tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg
    54121 gctgtttttg gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt
    54181 ccaacctccg gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta
    54241 ctgatagtca gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg
    54301 aagatttctg ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc
    54361 cattgctcgc catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg
    54421 gcattgctca ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg
    54481 ctcggtacat catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg
    54541 ctcattaatt gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact
    54601 gaaacatttt tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc
    54661 agcaacgatt cgccagagtc ggtaatcgcc cgcaaatcat cacgctgggc gttaagtgcg
    54721 gggttatctg ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca
    54781 tggctcatcg ccgccagaaa cgccgatttt gcctagctgg ctttttctgc ttccgcccgt
    54841 gcctgtcggt gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc
    54901 gccagctgtt cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc
    54961 cgcccgatgg tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca
    55021 ccgtccagca gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gactgagcga
    55081 taaaccacgc gccagaggat cagaatcagt gcgcaaagtg aaaccatccc cagcaataac
    55141 aggctgtatt gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgcgc
    55201 agctcagtgg tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata
    55261 ttattttggg cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc
    55321 aacagatcgc tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc
    55381 ggatcttcaa tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt
    55441 tccagcgttg gtgcattttt ctggagctgc tccagcccca gattcatcac catttgctgc
    55501 acccgcagag cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca
    55561 atcagccgat cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa
    55621 atcccggcct gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgcgccagg
    55681 cgtgcgatct catcggcggc agctatctgc tgactgagtt gccgttgttg ctggcgtagt
    55741 tgcagacgcc gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc
    55801 tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta
    55861 atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg
    55921 gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc
    55981 gccatattca tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag
    56041 cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag
    56101 cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag
    56161 gttgagtcgc aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg
    56221 cgcgtactgc tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac
    56281 ctgttgcgct ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc
    56341 aaacgcgtat ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg
    56401 ttgaactatg gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag
    56461 gcaggcggct acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg
    56521 ggcgcagagg ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag
    56581 gtagcaaatc tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc
    56641 cgcgttggtg taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg
    56701 gcgcacggta acccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt
    56761 aaggagatgg tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat
    56821 attgcgcttg gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa
    56881 cgccacccag aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt
    56941 gaagggcgta acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg
    57001 tatcgcgggc tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag
    57061 ggggaactgg cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat
    57121 gtcagcccac cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc
    57181 tcgctgtcac cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag
    57241 aaataacctt caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga
    57301 cgaattaaca catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata
    57361 cgcagcagac gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt
    57421 tcatactctg cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac
    57481 acattcaggc aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt
    57541 cgcgcgaggt cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc
    57601 agcggtttgg tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga
    57661 tcgctgcgtc cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt
    57721 aacatcaggc cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga
    57781 ttctgcataa tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc
    57841 tgagtgaagt aggattgtaa tcgcgcctgg gtaaccggct catcttcaac aataacaatg
    57901 tgatgtggca tcagagggtt ttactcattc tgttcatatc tgttcatatt ctgccgtaag
    57961 ccgttcatcc tgaccagtgc cgctgttcat atttgctcat taagatcgct tcactaaacc
    58021 ataattctac aggggttatt atgcggaaac tctggaacgc gctacgccga cccagtgctc
    58081 gttggtcggt actggcgctg gtcgcaattg ggattgtgat tggcattgcg ctgattgtat
    58141 tgccacacgt tgggatcaaa gtcaccagca caaccgaatt ttgtgtcagt tgccacagta
    58201 tgcaaccggt gtatgaagaa tataaacagt cggtgcattt ccagaacgcc tccggcgtgc
    58261 gagctgaatg ccatgactgt catatcccgc cggatattcc aggcatggtg aagcgcaaac
    58321 tggaagcgag caacgacatt taccagacct ttattgccca ctccattgat acacctgaaa
    58381 aattcgaagc caaacgcgcg gaacttgccg agcgtgaatg ggcgcgaatg aaagaaaaca
    58441 actcggcaac ctgtcgctcc tgccataact acgatgcgat ggatcatgcc aggcagcatc
    58501 ctgaagcggc acgtcagatg aaggtggcag cgaaagataa tcaatcctgc atcgactgtc
    58561 ataaaggtat tgcccaccag ttaccggata tgagtagcgg cttccgtaag cagttcgatg
    58621 agctgcgcgc cagtgctaat gacagtggtg acacgctgta ctctattgat attaagccaa
    58681 tttatgcggc gaaaggcgat aaagaagcct ctggttctct gctgcctgct tcggaagtga
    58741 aagtccttaa acgtgacggc gactggctgc aaattgaaat taccggctgg acggaaagcg
    58801 ccggacgtca gcgtgtactc acccaattcc caggtaaacg catctttgtt gcctcgattc
    58861 gtggtgatgt gcagcagcag gtgaaaacgc tggagaaaac caccgttgcc gacaccaata
    58921 ccgagtggag caagttgcag gccaccgcgt ggatgaagaa aggcgacatg gtgaacgata
    58981 tcaaaccgat ctgggcctat gcggattcgt tgtacaacgg cacctgtaac cagtgccacg
    59041 gcgcaccgga aatcgcccac tttgacgcta acggttggat cggcacgctc aacggcatga
    59101 ttggctttac cagtctcgat aaacgtgaag aacgcacttt gttgaaatat ctgcaaatga
    59161 atgcgtctga caccgcaggt aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa
    59221 taacgatctc tttcaggcat cacgtcggcg ttttctggca caactcggcg gcttaaccgt
    59281 cgccgggatg ctggggccgt cattgttaac gccgcgccgt gcaactgcgg cgcaagcggc
    59341 gactgaggct gtcatctcga aagagggcat tcttaccggg tcgcactggg gggctatccg
    59401 cgcgacggtg aaggatggtc gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc
    59461 gtcgaaaatg atcgccggat tgccggatca cgtacacaac gcggcacgta ttcgttatcc
    59521 gatggtacgc gtggactggc tgcgtaagcg ccatctgagc gacacctccc agcgcggtga
    59581 taaccgtttt gtgcgcgtga gctgggatga agccctcgac atgttctatg aagaactgga
    59641 acgcgtacag aaaactcacg ggccgagtgc cttgctgacc gccagtggtt ggcaatcgac
    59701 ggggatgttc cataacgctt cggggatgct ggcgaaagct attgccttgc atggtaatag
    59761 cgttggtacg ggcggagatt actctaccgg tgctgcgcag gtgatcctgc cgcgcgtagt
    59821 cggttcaatg gaagtgtatg aacagcaaac ctcctggccg ctggtattgc agaacagcaa
    59881 aaccattgtg ctgtggggct ccgatttgct gaaaaaccag caagcgaact ggtggtgccc
    59941 ggatcacgat gtttatgaat attacgagca gctaaaagcg aaagtcgccg ccggtgaagt
    60001 tgaggtcatc agcatcgatc cggttgtcac atccacccat gagtatctgg ggcgcgagca
    60061 tgtgaagcac attgcggtta acccgcaaac tgacgtgccg ctgcaactgg cgctggcaca
    60121 tacgctgtac agtgaaaacc tgtacgacaa aaacttcctc gctaactact gtgtgggttt
    60181 tgagcagttc ctgccgtatc tgctgggtga gaaagacggt cagccgaaag atgccgcatg
    60241 ggctgaaaaa ctgaccggca ttgatgccga aaccattcgt gggctggcgc ggcagatggc
    60301 ggcgaacaga acgcaaatta ttgctggctg gtgcgtgcag cgtatgcagc acggtgaaca
    60361 gtgggcgtgg atgattgtgg ttctggcggc gatgctgggg caaattggcc tgccaggtgg
    60421 tggttttggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
    60481 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
    60541 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
    60601 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
    60661 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
    60721 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
    60781 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
    60841 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg cagttcgagg cgcgcaacga
    60901 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
    60961 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
    61021 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
    61081 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
    61141 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
    61201 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
    61261 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
    61321 gcagttatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
    61381 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
    61441 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    61501 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    61561 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcggtactt cgcagctggc
    61621 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    61681 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    61741 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    61801 ctggcgcagt tgttctcccg tgaactggac gatgaacaac tgacgcaaat cgccagtgcg
    61861 cagatggctg aatggttttc gttgctgaaa agcgaaccgc cgctcactgc ggcggtgaat
    61921 gatctggaaa acagtattgc cacgctgaca gttcgtgacg atgcccgtct ggaactggcc
    61981 gctgactttt gcggcctgtt tctgatgacc gacaaacaag tggcgctgcc gtatgcatcg
    62041 gcctacaaac aggacgagca agagattaaa cgcttgttag ttgaggcagg gatggaaacc
    62101 agtggcaatt tcaacgaacc ggcagatcat ctggcgatct atctcgagtt gttgagtcat
    62161 ctgcattttt cgctgggaga ggggaccgtt cctgcgcgaa gaatcgacag tttgcggcaa
    62221 aaaacactga cggcgctgcg gcaatggtta ccagagtttg ctgcgctttg tcgacagtat
    62281 gacagctttg gtttttacgc ggcactaagc cagttattgc tggtgttagt ggagtgcgac
    62341 caccaaaaca gataacgtcg tttatgcgcc tgaaaagacg cgtttagcgt cgcatcaggc
    62401 attatggcgc agttgccgga tgcgtcatca catccggcaa acacacatca cggatgagct
    62461 acaaaccggg aaagccgctg gcgtagcagg cggttttcct gcttcaggtg cgcaatatca
    62521 tccattaacg tcagcgccac cgcgatcccc ggccagtcca gagccagttc atgacgcagg
    62581 cgtaccgcgc gttgcaccac aatggcggca tggtcgtcaa atacccaggt tgtttcctgg
    62641 atctcacgcg gttcaaccac ccccaaaccg acaatttcat tcaactcctc ttcagagatg
    62701 ccggtatgca ggcaaaattc ggtaatagta aaagtcaccg taacattagc cattatgctt
    62761 tcccccaatc tttacgtgga tcaaaagacg actgggcgtc tgccagttgc tgccacagcg
    62821 cggcagtgtt ttcatccggt ttcggcggca tcacgatttt cagtaccgca tacagatcgc
    62881 cggtctgttt tttgctcacc agacctttgc ctttaacgcg caatcgttgc ccggcctggc
    62941 tgcctggcgg gatagtcagc aaaatgcttt ctttcagtgt tggaacggtg actttagtac
    63001 ccagcgccgc ttcccacggg ctaaccggca ccacaatttc cagatcctgg ccgacaatat
    63061 cgaacagcgg atgtggcgca atatgaatca ccagccacaa atcgccattt ggaccgccgt
    63121 tttcgcccgg cgccccctgg cctttcaggc ggatgcgttg accattgccg acgcctgccg
    63181 ggatcttcac attcagcgtt ttcgggattt cctgttcgat catgccaaag gcgttataaa
    63241 ccggcaggtt atagctgatg gtacgcttat gctcagtaag cgtttcttcg aggaataccg
    63301 ccacttcgat ttcaatatcg tggccgcgtg cggcggggcg ttgacggctc tggcgggcat
    63361 gctgaccgaa aattgacgag aagatatcgt caaaatcttc ggcattaaaa ctctgaccgt
    63421 cgccatgatg gaactgacgg ttaaattgcg gatcgttgcg atgttgccac atctgatcat
    63481 actcagcgcg acgttgttca tcacttaaca cttcccaggc ttcagcgacc tctttgaagc
    63541 gggcttcggc atccggttct ttgctgacat caggatggta tttacgggcc agtcgacgat
    63601 aggcggtctt gattgtcttg agatcgtccg tcggtttcac gcccatgatg gcgtaataat
    63661 cctttaattc catagcgtta tctcgcgtaa atcaacacaa attgaaggaa cccctgtaag
    63721 gtaactccta taagtgtagg gtaatcctca aaatttcata tgccaacaca gaatatgtta
    63781 ttgaaatcat cgcggagagg aggtcgccat caagatgggt tgctgaacat attttaaaca
    63841 ggtgaaaaag ggtgagcgat ttttgatagt tgaaccaggc actttaagtt
//
LOCUS       DADLSE010000006        62422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLSE010000006 DADLSE010000000
VERSION     DADLSE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.006"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            216..719
                     /locus_tag="JN692_000380"
     CDS             216..719
                     /locus_tag="JN692_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7262785.1"
                     /translation="MGMIGYFADIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN692_000381"
     CDS             complement(786..2243)
                     /gene="nuoN"
                     /locus_tag="JN692_000381"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7262786.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN692_000382"
     CDS             complement(2250..3779)
                     /gene="nuoM"
                     /locus_tag="JN692_000382"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7262787.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN692_000383"
     CDS             complement(3943..5784)
                     /gene="nuoL"
                     /locus_tag="JN692_000383"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7262788.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN692_000384"
     CDS             complement(5781..6083)
                     /gene="nuoK"
                     /locus_tag="JN692_000384"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7262789.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN692_000385"
     CDS             complement(6080..6634)
                     /gene="nuoJ"
                     /locus_tag="JN692_000385"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7262790.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN692_000386"
     CDS             complement(6646..7188)
                     /gene="nuoI"
                     /locus_tag="JN692_000386"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7262791.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN692_000387"
     CDS             complement(7203..8180)
                     /gene="nuoH"
                     /locus_tag="JN692_000387"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7262792.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN692_000388"
     CDS             complement(8177..10903)
                     /gene="nuoG"
                     /locus_tag="JN692_000388"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7262793.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN692_000389"
     CDS             complement(10956..12293)
                     /gene="nuoF"
                     /locus_tag="JN692_000389"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7262794.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN692_000390"
     CDS             complement(12290..12790)
                     /gene="nuoE"
                     /locus_tag="JN692_000390"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7262795.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN692_000391"
     CDS             complement(12793..14595)
                     /gene="nuoC"
                     /locus_tag="JN692_000391"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7262796.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPTAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14689..15351)
                     /locus_tag="JN692_000392"
     CDS             complement(14689..15351)
                     /locus_tag="JN692_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7262797.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN692_000393"
     CDS             complement(15367..15810)
                     /gene="nuoA"
                     /locus_tag="JN692_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7262798.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN692_000394"
     CDS             complement(16441..17379)
                     /gene="lrhA"
                     /locus_tag="JN692_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7262799.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKSGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18299..19516
                     /locus_tag="JN692_000395"
     CDS             18299..19516
                     /locus_tag="JN692_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7262800.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19600..20199
                     /gene="yfbR"
                     /locus_tag="JN692_000396"
     CDS             19600..20199
                     /gene="yfbR"
                     /locus_tag="JN692_000396"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7262801.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20258..22090)
                     /locus_tag="JN692_000397"
     CDS             complement(20258..22090)
                     /locus_tag="JN692_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7262802.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASTAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN692_000398"
     CDS             complement(22177..22827)
                     /gene="hxpA"
                     /locus_tag="JN692_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7262803.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(22838..23332)
                     /locus_tag="JN692_000399"
     CDS             complement(22838..23332)
                     /locus_tag="JN692_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7262804.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23415..23870)
                     /locus_tag="JN692_000400"
     CDS             complement(23415..23870)
                     /locus_tag="JN692_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7262805.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24208..25410
                     /gene="ackA"
                     /locus_tag="JN692_000401"
     CDS             24208..25410
                     /gene="ackA"
                     /locus_tag="JN692_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7262806.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25485..27629
                     /gene="pta"
                     /locus_tag="JN692_000402"
     CDS             25485..27629
                     /gene="pta"
                     /locus_tag="JN692_000402"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7262807.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEAPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27819..29339
                     /gene="yfcC"
                     /locus_tag="JN692_000403"
     CDS             27819..29339
                     /gene="yfcC"
                     /locus_tag="JN692_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7262808.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN692_000404"
     CDS             complement(29372..29914)
                     /gene="yfcD"
                     /locus_tag="JN692_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7262809.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN692_000405"
                     /pseudo
     CDS             complement(29972..30522)
                     /gene="yfcE"
                     /locus_tag="JN692_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
     gene            complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN692_000406"
     CDS             complement(30578..31222)
                     /gene="yfcF"
                     /locus_tag="JN692_000406"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7262810.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31358..32005
                     /gene="yfcG"
                     /locus_tag="JN692_000407"
     CDS             31358..32005
                     /gene="yfcG"
                     /locus_tag="JN692_000407"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7262811.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKWLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32062..32424
                     /gene="folX"
                     /locus_tag="JN692_000408"
     CDS             32062..32424
                     /gene="folX"
                     /locus_tag="JN692_000408"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7262812.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32445..33338
                     /locus_tag="JN692_000409"
     CDS             32445..33338
                     /locus_tag="JN692_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7262813.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33386..34276)
                     /locus_tag="JN692_000410"
     CDS             complement(33386..34276)
                     /locus_tag="JN692_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7262814.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN692_000411"
     CDS             complement(34473..35246)
                     /gene="hisP"
                     /locus_tag="JN692_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7262815.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35254..35970)
                     /locus_tag="JN692_000412"
     CDS             complement(35254..35970)
                     /locus_tag="JN692_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7262816.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILILHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(35967..36653)
                     /locus_tag="JN692_000413"
     CDS             complement(35967..36653)
                     /locus_tag="JN692_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7262817.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN692_000414"
     CDS             complement(36743..37525)
                     /gene="hisJ"
                     /locus_tag="JN692_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7262818.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN692_000415"
                     /pseudo
     CDS             complement(37746..38528)
                     /gene="argT"
                     /locus_tag="JN692_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
     gene            complement(38794..39363)
                     /locus_tag="JN692_000416"
     CDS             complement(38794..39363)
                     /locus_tag="JN692_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7262819.1"
                     /translation="MKRLIVGISGASGAIYGVRVLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN692_000417"
     CDS             complement(39458..40975)
                     /gene="purF"
                     /locus_tag="JN692_000417"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7262820.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGVIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN692_000418"
     CDS             complement(41012..41500)
                     /gene="cvpA"
                     /locus_tag="JN692_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7262821.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN692_000419"
     CDS             complement(41759..42421)
                     /gene="dedD"
                     /locus_tag="JN692_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7262822.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPLAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN692_000420"
     CDS             complement(42411..43679)
                     /gene="folC"
                     /locus_tag="JN692_000420"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7262823.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELLE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN692_000421"
     CDS             complement(43749..44663)
                     /gene="accD"
                     /locus_tag="JN692_000421"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7262824.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(44819..45478)
                     /locus_tag="JN692_000422"
     CDS             complement(44819..45478)
                     /locus_tag="JN692_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7262825.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN692_000423"
     CDS             complement(45561..46373)
                     /gene="truA"
                     /locus_tag="JN692_000423"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7262826.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46373..47386)
                     /locus_tag="JN692_000424"
     CDS             complement(46373..47386)
                     /locus_tag="JN692_000424"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7262827.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN692_000425"
     CDS             complement(47452..48588)
                     /gene="pdxB"
                     /locus_tag="JN692_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7262828.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQVRLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48687..49682
                     /locus_tag="JN692_000426"
     CDS             48687..49682
                     /locus_tag="JN692_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7262829.1"
                     /translation="MIQPISGPPPRQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49679..50857)
                     /locus_tag="JN692_000427"
     CDS             complement(49679..50857)
                     /locus_tag="JN692_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7262830.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQSLTGALTWGLGIVGLKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN692_000428"
     CDS             complement(51132..52352)
                     /gene="fabB"
                     /locus_tag="JN692_000428"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7262831.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52511..54517
                     /gene="mnmC"
                     /locus_tag="JN692_000429"
     CDS             52511..54517
                     /gene="mnmC"
                     /locus_tag="JN692_000429"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7262832.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLFDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54638..54916)
                     /locus_tag="JN692_000430"
     CDS             complement(54638..54916)
                     /locus_tag="JN692_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7262833.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(54950..55498)
                     /locus_tag="JN692_000431"
     CDS             complement(54950..55498)
                     /locus_tag="JN692_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7262834.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55498..56307)
                     /locus_tag="JN692_000432"
     CDS             complement(55498..56307)
                     /locus_tag="JN692_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7262835.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEYRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN692_000433"
     CDS             complement(56307..57131)
                     /gene="mepA"
                     /locus_tag="JN692_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7262836.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN692_000434"
     CDS             complement(57135..58220)
                     /gene="aroC"
                     /locus_tag="JN692_000434"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7262837.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN692_000435"
     CDS             complement(58255..59187)
                     /gene="prmB"
                     /locus_tag="JN692_000435"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7262838.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNQWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTCRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59353..59904
                     /gene="smrB"
                     /locus_tag="JN692_000436"
     CDS             59353..59904
                     /gene="smrB"
                     /locus_tag="JN692_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7262839.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60008..60898)
                     /locus_tag="JN692_000437"
     CDS             complement(60008..60898)
                     /locus_tag="JN692_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7262840.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSVAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(60885..61409)
                     /locus_tag="JN692_000438"
     CDS             complement(60885..61409)
                     /locus_tag="JN692_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262841.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALSGFL
                     AFDSSSSASGAGIGIETAAGTSVPVNNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61406..61876)
                     /locus_tag="JN692_000439"
     CDS             complement(61406..61876)
                     /locus_tag="JN692_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262842.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(61873..62421)
                     /locus_tag="JN692_000440"
     CDS             complement(61873..62421)
                     /locus_tag="JN692_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7262843.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
BASE COUNT        15547 a        17017 c        15625 g        14233 t
ORIGIN      
        1 tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga
       61 aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa taataaaaat
      121 ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg tctgttttaa
      181 gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc tattttgcag
      241 acattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata
      301 atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt
      361 tacactttgg gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc
      421 gtgccgtgct tgatgaacac agcctcgaag atggcataga tggcttcctg ggattgacct
      481 ggaatcagga attagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc
      541 gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt
      601 cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa aagctgatta
      661 gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat
      721 ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta
      781 acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg tggccataca
      841 cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc
      901 cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag gctcaccgcc
      961 acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac
     1021 cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc
     1081 atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga
     1141 tgccagaaca aaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg
     1201 gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa
     1261 acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga
     1321 tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac
     1381 gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc
     1441 gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg
     1501 attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct
     1561 ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcatg
     1621 ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc
     1681 gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaaa
     1741 gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg
     1801 cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac
     1861 agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc aattaacacc
     1921 aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta
     1981 caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg
     2041 cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag cgaaaccagc
     2101 gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca
     2161 atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc
     2221 aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca
     2281 aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag
     2341 aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac
     2401 atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc
     2461 aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatt
     2521 accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg
     2581 ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc
     2641 ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga
     2701 ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg
     2761 ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc catgtgggaa
     2821 accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat ccacgcaccg
     2881 tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc cgacgcgttc
     2941 gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag caagatcccc
     3001 gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc
     3061 agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat caacagatat
     3121 tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg
     3181 gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc
     3241 gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct
     3301 ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag
     3361 aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc
     3421 aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc
     3481 agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc aatggcgaga
     3541 tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga
     3601 attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc
     3661 agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg
     3721 gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg
     3781 gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac
     3841 agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag cttatccgac
     3901 ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa
     3961 cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc gcagataacc
     4021 gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg ggatgttcat
     4081 cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa
     4141 cactttgtcg tacagccagt caaagcccca ggcgttgtac caccaggtac tcagcaggcg
     4201 gcccggcgca ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc
     4261 tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct
     4321 gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc
     4381 gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt
     4441 cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt
     4501 gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt
     4561 cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag
     4621 tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg
     4681 cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga
     4741 tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc
     4801 ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc
     4861 gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac
     4921 cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt
     4981 acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac
     5041 aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt
     5101 accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc
     5161 aaagtgcgct ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta
     5221 aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt
     5281 cattgccgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca
     5341 caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac
     5401 taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc
     5461 gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag
     5521 catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga
     5581 catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc
     5641 gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac
     5701 gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat
     5761 aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc
     5821 tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg
     5881 aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg
     5941 accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc
     6001 agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc
     6061 agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt
     6121 tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca
     6181 gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg
     6241 taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga
     6301 ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg ccaatccaca
     6361 cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccaag ttgagcatca
     6421 tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac
     6481 cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca
     6541 ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg
     6601 ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct
     6661 cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc
     6721 catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag
     6781 atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt
     6841 taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga
     6901 gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ctttttgcag
     6961 cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc
     7021 gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg
     7081 ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat
     7141 actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc
     7201 ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc
     7261 ggcaggcaga ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac
     7321 gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat
     7381 ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg
     7441 gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca
     7501 atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga
     7561 cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc
     7621 cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc
     7681 acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc
     7741 atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa
     7801 accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc
     7861 caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc
     7921 ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac
     7981 attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtcca
     8041 taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccacag
     8101 gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt
     8161 tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca
     8221 gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagtcctt
     8281 cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac
     8341 ctgcgttcac acccaacttc gcggcatccg ctgggttgag cttgatgtac ggctgcggca
     8401 tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtagt
     8461 acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt
     8521 aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt
     8581 tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc
     8641 aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg
     8701 tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca
     8761 tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga
     8821 atgtcgcatc cggcgcagca tctttgatac ccgccagttc cgggattttc gccacaacag
     8881 cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag gtgctgtgca
     8941 gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagccggat
     9001 cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt
     9061 cagcaaagct ggcagcggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat
     9121 gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcagaag
     9181 cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg gtttccagtt
     9241 cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg
     9301 aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt
     9361 tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt
     9421 tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc tcaggttcga
     9481 taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg
     9541 cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat
     9601 caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa
     9661 tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct ttcaccgcct
     9721 gacgcactgc cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta
     9781 ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca
     9841 ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt
     9901 cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa
     9961 taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg
    10021 cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca
    10081 ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac
    10141 cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac
    10201 agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt
    10261 aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac
    10321 cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg
    10381 cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt
    10441 agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt
    10501 gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct
    10561 gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta
    10621 accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg
    10681 tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt
    10741 tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc
    10801 agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc
    10861 cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt
    10921 tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc
    10981 ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat
    11041 tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca
    11101 cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc
    11161 ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg
    11221 cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag gttacgcacc
    11281 agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctg
    11341 cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag
    11401 tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg cataccaccg
    11461 gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga
    11521 tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg
    11581 ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg
    11641 ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg gggtttcgag
    11701 cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg
    11761 tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc cataatgttt
    11821 ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt tactgccgct
    11881 tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag
    11941 atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag
    12001 gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct
    12061 ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga
    12121 ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc
    12181 gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg
    12241 cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc
    12301 gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca
    12361 tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcggc
    12421 catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac
    12481 cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa
    12541 ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa
    12601 tacccagcac atcggcgatc gcgtggatcg caccatcagg cacccagcca cgctgcttct
    12661 gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt
    12721 gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat
    12781 tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac
    12841 aatcaggtca gacaccaggc tgccgcggat cgccgtcgga atttgctgca aatgcgcata
    12901 actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta
    12961 actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg
    13021 cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc
    13081 tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt
    13141 gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg
    13201 ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc
    13261 agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc
    13321 agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc
    13381 tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca
    13441 gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg ctacgccgcc
    13501 aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat
    13561 tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa
    13621 ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat
    13681 gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg
    13741 catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg
    13801 ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac
    13861 gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga
    13921 cgggtggttc ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat
    13981 cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt
    14041 cagctcaaac ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc
    14101 tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc
    14161 aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt
    14221 cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc
    14281 gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt
    14341 gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt
    14401 ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc
    14461 ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat
    14521 taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt
    14581 catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa
    14641 tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc
    14701 aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg
    14761 catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt
    14821 gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca
    14881 gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat
    14941 atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag
    15001 catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac
    15061 caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac
    15121 gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt
    15181 atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa
    15241 cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg
    15301 gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc
    15361 tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg
    15421 tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag
    15481 taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga
    15541 ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac
    15601 cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc
    15661 aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag
    15721 catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc
    15781 gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc
    15841 actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt
    15901 acaatcactg tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat
    15961 aaccttttgt caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat
    16021 tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt
    16081 gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg
    16141 tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt tgttattcca
    16201 taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt
    16261 ttcaggcgat tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa ataaacagtt
    16321 ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa
    16381 aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga
    16441 ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct
    16501 gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt
    16561 attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc
    16621 tactccactc agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc
    16681 aagaccggat ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg
    16741 ccatggaata tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc
    16801 atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta
    16861 ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac gatgggtggt
    16921 taccatcaaa tccacttcct gggattccag catttctgcc atataggcat tacgtttaac
    16981 gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa
    17041 gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa
    17101 cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag
    17161 ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc
    17221 aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc
    17281 ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc
    17341 gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc
    17401 tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc
    17461 tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa
    17521 aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg
    17581 gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata
    17641 aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg
    17701 tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat gcgagcattt
    17761 taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt
    17821 atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa
    17881 ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct
    17941 tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat
    18001 atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac
    18061 agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata acattgcatg
    18121 taaagcatat acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct
    18181 tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt
    18241 gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat
    18301 gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt
    18361 gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg
    18421 caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa
    18481 cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc gtaaagccat
    18541 catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg
    18601 caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga
    18661 aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc tttccagcgg
    18721 taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat
    18781 tcgcgctaaa attacgcctc gtacgcgtgg gattgttatt atcaacccaa ataacccaac
    18841 cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa
    18901 tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg ctgagcatca
    18961 ctcaattgcg ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac
    19021 gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc
    19081 caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc
    19141 tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac
    19201 ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc
    19261 gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa
    19321 acgctttaac attcatgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt
    19381 tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac
    19441 gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg
    19501 ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag tgttatcccg
    19561 ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt
    19621 gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa cgtgcggacg
    19681 gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc
    19741 aaaaatcgga aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac
    19801 cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaattcg
    19861 caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcagaaact ggtcgatatg
    19921 gttccggaag aactgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat
    19981 gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa
    20041 gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt
    20101 gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat
    20161 ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca
    20221 tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga
    20281 tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt
    20341 cactgaagct gtaatttccc ggacctaaaa ccagggtgtt aacaggtgaa gacaccggtg
    20401 tcataaaggc ggcggatgct gccatcgcca cgaccatcgc aaatggataa ggcgacactc
    20461 ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag
    20521 agataaacag cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc
    20581 cataaccgcc gccaatatcc atcagccctt tcaccgccag cgcgacacct cccgttttct
    20641 gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg
    20701 atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga
    20761 tagcggcaac aggattagga atttcatctg tcagcattaa cgccaccatt agcaccagac
    20821 agaaaatggc atggggtgcc tggctgtgcg cggtcgatgc ttcactcacc tcttccggta
    20881 agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac
    20941 ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg
    21001 ccacgccatt gcgcttgagc cccaccacat tcagtccgta gcgggtacga aaaccaattt
    21061 cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca
    21121 catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct
    21181 cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac
    21241 gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc
    21301 gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt
    21361 gacctatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat
    21421 attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg
    21481 tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga
    21541 ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt
    21601 cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc
    21661 cggcaaaact taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc
    21721 ttaacaccac gggaataaag atagcgacaa cgccggttga actcataaac gcgccaagcc
    21781 ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga
    21841 ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa
    21901 acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg
    21961 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
    22021 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
    22081 caccgttcac aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag
    22141 ccccgacgag acgaggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
    22201 tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag
    22261 gcgcggggta tctgccggag cgttaaccgc aatgacatgg caacccgccg ccaggccaga
    22321 aagcacgcca gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag
    22381 ctgcgcacct aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt
    22441 tacaaacacc tctggtgcgg gaagccccgc tattttatgg cgcgctcgcg ctaccggcat
    22501 ggagccagaa gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc
    22561 gattgcccca ggaagcgcgg taataccttc ggtttccgtg gcctcgattt gctccagacg
    22621 cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt
    22681 gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct
    22741 ggcccagttg ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag
    22801 atcaaacaga aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa
    22861 tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct
    22921 gatatttttc ccacattggc gtctgggcgt taaaaccatg agtcccagcg tcaaaatggg
    22981 tatagcgccc ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg
    23041 cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat
    23101 tagaccagga aacatgcaac gcatgataca tctccataat gtcgatgata gtgcggcagg
    23161 tttcttcttt cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac
    23221 gctcaataat tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca
    23281 tcatcttgta ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact
    23341 cctgtatcac tctactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg
    23401 taatcagggg tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc
    23461 ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt
    23521 ccagactcct gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat
    23581 ggcgttacag aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac
    23641 agtgcagtgg caactgccgg cccaagcaga ccgcccaggg caatctgcca gcagagagta
    23701 aatacggcga tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt
    23761 tcgacaaaga ccggggcaag gcgtttttcc agtggccacg tctttgagta atgctgtccg
    23821 cggcgaaaca agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc
    23881 aacttcacat ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc
    23941 aaaacatttt gtcttccata cccactatca ggtatccttt agcagcctga aggcctaagt
    24001 agtacatatt cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc
    24061 aaaatggcat agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat
    24121 gttaatcata aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt
    24181 agccacgtat caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc
    24241 ggtagttctt cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct
    24301 ggtttagccg aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat
    24361 aaacaggaag cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt
    24421 aatactattc tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt
    24481 atcgtacacg gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag
    24541 ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc
    24601 gaagaagctc tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc
    24661 gcgttccacc agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac
    24721 aaagagcacg gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag
    24781 gaagcggcaa aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg
    24841 ggcaacggtg gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggt
    24901 ctgaccccgc tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc
    24961 atcttccacc tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc
    25021 aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac
    25081 aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg
    25141 aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcact
    25201 ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg
    25261 ctgggctttg aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc
    25321 atcaacaaag aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc
    25381 gcgcaagacg cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct
    25441 gttttgtaac ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta
    25501 tgctgatccc taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg
    25561 caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg
    25621 gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg
    25681 ccgctgaacc gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg
    25741 tgctgatgga agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc
    25801 tggttgaagg tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa
    25861 tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg
    25921 aacagctgaa agagcgtatc gaactgaccc gcaacagctt cggtggtgcc aaaaacacca
    25981 acatcaccgg cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc
    26041 gcccggatct gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc
    26101 cggcgaagct gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg
    26161 acctgatcgc gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg
    26221 aaggcgacat caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc
    26281 acatgctgga gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg
    26341 tgctggtggc cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga
    26401 ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg
    26461 gcctgccggt atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct
    26521 tcaacctgga agctccggtt gacgatcacg aacgtatcga gaaagttcag gaatacgttg
    26581 ctaactacat caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc
    26641 gtctgtctcc gccagcgttc cgttatcagc tgactgaact ggcgcgcaaa gcgggcaaac
    26701 gtatcgttct gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg
    26761 aacgtggtat cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag
    26821 cgtctcaggg tgtagaacta ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg
    26881 aaagctatgt tggtcgtctg gtcgagctgc gtaagaacaa aggcatgacc gaaaccgttg
    26941 cccgcgaaca gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag
    27001 ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc
    27061 agctgatcaa aactgcaccg ggcagctccc tggtatcttc cgtgttcttc atgctgctgc
    27121 cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc
    27181 tggcagaaat cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg
    27241 ttgctatgct ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc
    27301 gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc
    27361 agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg
    27421 caggtcgcgc taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag
    27481 cggtacagcg ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaaac
    27541 cggttaacga cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga
    27601 ctgcgattca gtctgcacag cagcagtaat ctcgtcatca tccacagctt tgcgctgcgg
    27661 atatctgaac cggaaataat cactatttcc ggttttttat tctcttaatt tgcattaatc
    27721 ctttctgatt atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat
    27781 acttaccact ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca
    27841 aaccaacaag aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt
    27901 taaccagtct tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt
    27961 atcaggttga tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc
    28021 tgactaacga agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg
    28081 aacgcccggg cctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga
    28141 cagccgttgg catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc
    28201 gtacaggaac cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa
    28261 ttctctttat tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg
    28321 gagaagaggc cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg
    28381 acagtattac caccgtcctg gtgacttata ttgccacgca aatcggtttt gccagttcgt
    28441 ggatgaaccc gttttgtgtg gttgttgctc aggggattgc cggcgttccg gtgctttctg
    28501 gctccgggtt gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca
    28561 tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc
    28621 gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc
    28681 tggtattgat tgtcctgaca gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg
    28741 cctggtttat tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca
    28801 tcggcgttgt tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg
    28861 aaggggcgcg aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc
    28921 tgctggttgg taatggtgaa gcaggtaatg ccagcgtgtt aaatactatc ctcaacagca
    28981 ttgccaatgc cattagcggt ctggacaacg cggtcgcggc ctggtttatg ttgctcttcc
    29041 aggctgtatt taatttcttc gtgacgtccg gttctggtca ggcggcgtta actatgccgt
    29101 tactggcacc gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc
    29161 agtttggtga tggcttcagc cacatcattt acccaacctc agcttcgtta atggcaacgc
    29221 tcggtgtgtg ccgggtggac ttccgtaact ggctgaaggt gggcgcgacc ctgcttggac
    29281 tgctgtttat tatgtccagc gtcgtagtga tcggcgctca gttgatgggc taccactaaa
    29341 aatgttaaga gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt
    29401 catttttggc attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact
    29461 catcgcagcg tgcggtgatt tcttccggcg tcagccagca aacttcactg acttcatctt
    29521 cctgtagggc gaagggaccg tgagagacgc aactgaacaa cgcgccccag acacggcaat
    29581 ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg gacaccggca atgcccaact
    29641 cttcttccgc ttcgcgacgc gcggattcca gcagttgctc atcggcctga actacaccgc
    29701 ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca
    29761 gaattttgcc catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg
    29821 cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca
    29881 cccattcagt acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg
    29941 ctcttacggc gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga
    30001 tactttgatc attgagtgcg attacactaa gtacgtcatt atccagcatg ccataactcg
    30061 ccggattacc gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc
    30121 gctgttctgc cactggcaaa tgggtatgac cgtacaccag cacatcgttc tggtttaaag
    30181 caggtagatt ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca
    30241 gtaatacctg ttgccacggc gcggttatcg ggaaatgcag catcatttga tccacttcgc
    30301 tgtcgcagtt gccacgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga
    30361 ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg ccgtgattca acacgtcgcc
    30421 aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac gttccgtcgc
    30481 cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact cctcgtcgaa
    30541 aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc ttcgccgaaa
    30601 gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa tccaccaggc
    30661 gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc agatcagtat
    30721 cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa tgttctgcca
    30781 tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct tttttcgccc
    30841 ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg ctgcgcagcc
    30901 aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa atacgttccc
    30961 aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat tcactcaact
    31021 caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct tgccacgtcg
    31081 gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac aggccttttt
    31141 cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca tctgaccaaa
    31201 gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc tatcaaacta
    31261 tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc gctttctata
    31321 cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct atttcgcccc
    31381 gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg attatcgctt
    31441 gattaaggta gacctgggga aaggtggtca gtttcgtccg gaatttttgc gcatttcgcc
    31501 caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg aaccgttaag
    31561 cctctttgag tctggtgcca ttttgttgta tctggctgag aaaacaggac tctttttgag
    31621 tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg taggcggact
    31681 ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa ccattcctta
    31741 cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa acaagtggct
    31801 ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg cctgctggcc
    31861 gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg cagtcaagaa
    31921 ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa aagcacaact
    31981 cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt tcatcctaag
    32041 taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat aaagaacctt
    32101 cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca ggatattgtt
    32161 atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga tatcaacgat
    32221 gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa taaccgtttc
    32281 tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca tcactgggtg
    32341 acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga ttcggtatcc
    32401 atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac atagtgatca
    32461 ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag ctgggccatc
    32521 aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc cctcgggtga
    32581 cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg gtaatcaacc
    32641 tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag cgtctctgcc
    32701 aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgccagc gacacgccgc
    32761 cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt gaagtggtgg
    32821 ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc cgctgggaag
    32881 agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga accggcgtgg
    32941 tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt cttggcctgg
    33001 gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat gatatggtca
    33061 acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg gtttcgccct
    33121 acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat cgcccggcta
    33181 ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca gtactggtat
    33241 taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg tttcgctggt
    33301 acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag caaacatccg
    33361 ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg tctggtgaaa
    33421 gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc tcctgagcaa
    33481 tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat ctgtcagcaa
    33541 tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca ataaatgcac
    33601 ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt agctgtctgt
    33661 cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc aacagatcgc
    33721 gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt tgcataatct
    33781 cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat attttgcggg
    33841 ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa ggacttctgc
    33901 aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc gcatcaagat
    33961 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
    34021 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
    34081 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttctagtt
    34141 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
    34201 tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
    34261 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
    34321 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
    34381 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccttgaaaa
    34441 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
    34501 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
    34561 ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc catttcgtga
    34621 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg taacacttcg
    34681 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
    34741 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
    34801 tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac cgcccgctcg
    34861 cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccaac
    34921 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
    34981 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
    35041 atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa gttaatgcag
    35101 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
    35161 ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt gtgcaaatcg
    35221 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
    35281 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
    35341 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
    35401 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
    35461 tatcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
    35521 aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt caatttcccc
    35581 atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
    35641 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
    35701 tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact gaacatacag
    35761 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
    35821 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
    35881 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
    35941 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
    36001 cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag gtaaatcacg
    36061 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ctgcgtggct
    36121 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
    36181 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
    36241 aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc tttcggcact
    36301 gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
    36361 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
    36421 atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa gtccggcacg
    36481 cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
    36541 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
    36601 tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa catgctgcct
    36661 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
    36721 ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag tactttttcg
    36781 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcctcgc
    36841 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
    36901 ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg aggaaacctt
    36961 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
    37021 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc cagtgttcat
    37081 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
    37141 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg gcagcgtaca
    37201 gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
    37261 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg ttttcgacaa
    37321 acgtacattg cgtattgatg cgcttgcaca actccttcgc caactcgata tcgaaaccaa
    37381 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
    37441 ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc agaaccagag
    37501 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
    37561 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
    37621 cccgttagac ttattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
    37681 tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
    37741 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
    37801 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
    37861 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
    37921 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
    37981 tcttgtaacg cagcatccag acgccctgca gccagatcgg aatagaccaa atcctggttg
    38041 gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta agcttcctgg
    38101 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
    38161 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
    38221 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc gatttttttc
    38281 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cctaggtaca tttcacctgc
    38341 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
    38401 tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
    38461 gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc gagaatcgac
    38521 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
    38581 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
    38641 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
    38701 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
    38761 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
    38821 atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
    38881 atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa tcaccgcacc
    38941 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
    39001 gcagagtacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
    39061 atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat
    39121 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt
    39181 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
    39241 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
    39301 taatacgcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
    39361 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
    39421 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
    39481 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
    39541 ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
    39601 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
    39661 cctggaaaat caccccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
    39721 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
    39781 ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
    39841 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
    39901 ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc agctgctggc
    39961 ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggtt
    40021 tgcccagaat acgtgcaatt tccagcgcga tatcacacga ggtttctggg atcgggatca
    40081 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt ttcgtgccca
    40141 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
    40201 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
    40261 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
    40321 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
    40381 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
    40441 taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag cggtttgtgg
    40501 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
    40561 cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
    40621 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
    40681 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
    40741 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
    40801 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
    40861 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
    40921 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
    40981 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
    41041 tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc agctgtgatt
    41101 tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
    41161 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
    41221 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
    41281 gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg tcttcaaagc
    41341 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
    41401 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
    41461 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
    41521 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
    41581 ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
    41641 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
    41701 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
    41761 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
    41821 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
    41881 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
    41941 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
    42001 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaagtggtg
    42061 cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
    42121 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
    42181 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
    42241 gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc accagcggga
    42301 tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
    42361 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
    42421 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
    42481 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
    42541 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
    42601 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
    42661 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
    42721 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
    42781 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
    42841 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgcc
    42901 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
    42961 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
    43021 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
    43081 acaattgccg gtttttcgct gcggaagatg cctgctttct cgcgaccaat actttcgcga
    43141 tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
    43201 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
    43261 agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
    43321 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccagc
    43381 aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg cgaactgtag
    43441 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
    43501 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac gccaagacgc
    43561 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
    43621 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
    43681 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
    43741 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
    43801 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
    43861 aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca
    43921 ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    43981 gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat ggcgaaactt
    44041 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    44101 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    44161 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttcctgcgcc
    44221 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    44281 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    44341 agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt atacttcttg
    44401 gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc cacaaggctt
    44461 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    44521 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    44581 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    44641 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctagtt cgatgccagt
    44701 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    44761 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    44821 attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg cccggcaaaa
    44881 tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc atcgggattg
    44941 taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg ccgatcacgt
    45001 tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggggcgaacg
    45061 ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt ttctcataaa
    45121 actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga ttactgaata
    45181 acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca atcgcggcga
    45241 tcagcatcag caccaccatc atatggacgt tgagatcgtt ggtttccagt gatgccaacg
    45301 ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc agaccggttt
    45361 cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc gcgactagct
    45421 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    45481 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    45541 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    45601 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    45661 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    45721 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    45781 gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    45841 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    45901 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    45961 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    46021 taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa
    46081 cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    46141 cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc tgcgtcagta
    46201 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    46261 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    46321 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    46381 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    46441 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    46501 agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg aattcgttct
    46561 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    46621 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    46681 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    46741 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    46801 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    46861 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    46921 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    46981 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    47041 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    47101 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    47161 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    47221 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    47281 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    47341 tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    47401 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    47461 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    47521 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    47581 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    47641 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca gcgtaatgcg
    47701 gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat gcccaataaa
    47761 cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca gggtatagcc
    47821 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa gttccggctc
    47881 gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc aggtcagcag
    47941 cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc cgggcttcag
    48001 gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat ctttaaagag
    48061 tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg agcggaaatc
    48121 accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa tccctaacgc
    48181 ttccagtcgc acctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    48241 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga aaacatattc
    48301 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    48361 ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt taatgggttt
    48421 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    48481 tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac ccaaacggct
    48541 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    48601 tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc
    48661 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctaggc
    48721 agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    48781 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacaacaaa
    48841 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    48901 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    48961 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata
    49021 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    49081 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    49141 cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    49201 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    49261 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    49321 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    49381 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    49441 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    49501 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    49561 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    49621 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    49681 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    49741 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    49801 cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    49861 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    49921 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    49981 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    50041 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    50101 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    50161 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    50221 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    50281 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    50341 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    50401 tccatgacat cactttgcca gagtgtttta gccctacgat gcctaacccc caggtcagag
    50461 cgcctgtcag tgactggctt tcaccaaacc caagaatcaa acgcccgatg accaacaggg
    50521 caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    50581 gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga tcggccagtc
    50641 gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatgccga
    50701 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    50761 accctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaactgg
    50821 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    50881 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg ttggcaggat
    50941 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaactggt
    51001 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    51061 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccgg
    51121 cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    51181 ccgaagctgt tagacataac tgtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    51241 agacccgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    51301 tccagcatca gcagagagta gatagcttcc tgcacacccg cagcgcccag tgagtgaccg
    51361 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    51421 gccagctctt tcacgtcgcc aaccggagtc gaagtaccgt gagagttcag gtaatcgatt
    51481 ggggtatcaa cgccatgcat cgccatctgc atacaacgta ctgcgccttc gccagacgga
    51541 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtaa
    51601 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    51661 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    51721 tatttagtag acagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct
    51781 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    51841 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    51901 ttaaacggcg tggcgagaca ggcagaaacg ccggatgcca tcgctttagt gaccacatac
    51961 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52021 cgcggggaac cgccgccgga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    52081 tccggagaga ggcccgcatc agcgattgcc tgctccatag aaaggaatgc ataaatggat
    52141 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    52201 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    52261 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    52321 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    52381 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    52441 atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    52501 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    52561 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    52621 gacgcgttat gtttttctgg gaggtaacca attagaggta cgctttcctg agcatccaca
    52681 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    52741 gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    52801 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    52861 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    52921 ttgccatcgt ttattgttcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    52981 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    53041 ggacggcttt gcgccagcta aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    53101 ggcaaggttg gcgcgtccag gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    53161 ccgcggtttg caggaagccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    53221 ggaaatgctt tgtggggtga tggaacagac attaccactc ccctgctcca cgccgtggtt
    53281 taatcgtacg ggcagcagca aacgggaagt agcgattatc ggcggtggta ttgccagcgc
    53341 gttgttgtcg ctggcgttat tacggcgcgg ctggcaggta gcgctttatt gcgcggatga
    53401 agcccccgcg ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    53461 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    53521 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    53581 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    53641 tgtagccgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    53701 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    53761 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    53821 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    53881 ggtactggcg aacgggcatc aaatcagccg tttcagccaa acgtcgactc tcccggtgta
    53941 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca
    54001 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    54061 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    54121 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    54181 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    54241 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcaaaa
    54301 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    54361 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    54421 accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg
    54481 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    54541 atgcggcgtg aacgccttat ccaacctacg tgtgacgtgt aggcctgata agacgcgata
    54601 agcgtcgcat caggcatggt gctccagacg ccgcaactta cagctgtgac gcctgctgaa
    54661 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    54721 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    54781 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    54841 acaactcatc atcactggca tggtcaacca tgccgtcgat taatgccaga atgcgtgatt
    54901 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcgctggctt cagttgagcg
    54961 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55021 ctttaatgaa gcgtgccggg cgttccggaa tgccatttgt cagataatcc atcacctgcg
    55081 catgtacccg ccgttgaaac accacacgat ccggttcgaa atccccttcc agattgtcgc
    55141 aactgacgtt aaacggatat ccagccgcca cgcagaataa ccagtcgaac gcctgcggct
    55201 tcacttcaac atcttcaaac tggctttgcg tctgggcatc gcgtccgtcc gggcaatacc
    55261 agtagccgaa atcaaccagc tcacggcgcg cttttccggc aatacaccag tgcgaaatct
    55321 catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg
    55381 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    55441 cggcaaagca gctattaaaa atttcaatca actgctcgta gtggtgtgta ctgttcatta
    55501 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcgtaaagta atttggcact
    55561 catcaccgcc gagacgataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    55621 caaccgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    55681 ccaaatcact ttgccgccga gaataaacag cagcagaccg ccaatgtttg acgttgcgtt
    55741 gagtaatttg gcgtgagccg tggctttggc gaggttgaat ccgcacagcg taacgaaggc
    55801 cagcgcgtaa aacgatccgg cagccgggcc aaagaatccg tcataaaaac cgacgcagcc
    55861 accggcaatt aacgcgaagg gtaaaccgta catccggcgc tggcgatatt cttcacccag
    55921 ctttggcatc agcaagaaat agaggccgat acaaatcacc agaatgggca aaatctgccg
    55981 taagacatca gcctgaacgt actgtaccaa cagtgcgccg ctcattgagc cgacaaaggt
    56041 catggcaata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    56101 agtggcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cgttagcggg
    56161 agacattccc gcagccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    56221 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggagaaac
    56281 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    56341 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    56401 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg atggcggtaa aggttgatct
    56461 tcacactcca gactatcggc agggcaacgt aatcgtacat gcatatgcgc gcgatgctgg
    56521 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatcaag gcaaagttgt
    56581 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtctt tgtcctgggc ggcgagtttg
    56641 atcaagctga aaatttctgg cttccacagc gtggagacaa cgtgtttacc gtctcgggaa
    56701 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    56761 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    56821 ccaccagcgg gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    56881 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    56941 tcggtacgca tgacctgata atgttcggac tgtatcggca acgtgtcagc gccgacaata
    57001 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    57061 tgccacggcg tcgctgccag gctagcgcta ctggcaagca gagccagcag cgcaatcgcg
    57121 gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc
    57181 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttcagcgat cggcactgca
    57241 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    57301 cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgagca
    57361 atgatttgct gcccgctgct gataccgccg agaatgccgc ccgcatggtt gctctggaaa
    57421 ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg
    57481 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca
    57541 tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg
    57601 actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg
    57661 tcgattttgt cggggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg
    57721 atttccagcg gaatgtcgcc catctgggtc aggcagccgc ggatttcaat accaaatttc
    57781 tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg
    57841 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca
    57901 gcatggcctg gacggaagac gtctttaatt gcgctgtaat cctgagaacg ctggtcggta
    57961 ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagagt
    58021 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    58081 cgatcaaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    58141 acgatgcagc cgagcgccag cccgtgcgat tcgccgaagg tggttacgcg aaagagttgt
    58201 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    58261 tttataaatc gcgaaatgtt ctcgtgcggc aataagctgc tctttagtga gcataaacac
    58321 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatcgggat attgttccat
    58381 aagatgtacc atgctgttgc ccacttcaca aatcaacacg ccatcatcag caaggtaatc
    58441 tgccgcgtta ccgagaatgc gacacgtcag tttcaggccg tcggtgccag atgccaggcc
    58501 cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata
    58561 cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga
    58621 acgaatcgga atgacattgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc
    58681 cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca
    58741 ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct
    58801 gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggcgagcgcg gcaccagcac
    58861 gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac
    58921 cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    58981 ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa
    59041 caccagctgt acggcttcat cccactggtt atcggtgccg tgaccgtacc agatatttgc
    59101 cgcgctgaag cggcttaccg accagcgcaa catgtcctga atggtttgca gctcatttac
    59161 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    59221 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    59281 aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat
    59341 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    59401 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    59461 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    59521 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    59581 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    59641 tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt
    59701 gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat
    59761 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac
    59821 caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag
    59881 tggttgccgc cggagttgcc ctgaatgctg gcagcaaatg ccggagcagc gtaaacgcct
    59941 tatccggcct acaaatatgt gataattcaa taaattgcaa ttcacttcgt aggcctgata
    60001 agacgcatca gagcaatact acgaacttca gttcattcaa tacatactga aaacgacacg
    60061 tcgaatcaat cggttgccca ctactggtat tcacattcac cacaatatcg tgtagtactt
    60121 tatccgttac cggatgacga gttggcgtaa ccgttaccgt tgatttatca caaccgtaag
    60181 aggtaaaggt taacaattct gataccgcgc ggcagtatgc tcctctgcca tcattatcag
    60241 aaaaattact gccatcacag agtctgccgc cttccatcag gtaggtcgaa agcgtggtgg
    60301 tcactgttga ccccgtggac tgaattcgta acgccttatt atggctgaca taattttcac
    60361 tccaggagat atcgtatgaa gcgaccggta tcgttgggca tattgtgtta tgcactgtag
    60421 gggccgtata gcttccaaat ccgggtaaat ctgtatagga ataaaaggat gcgatccaag
    60481 ggcaaccata atcatcagat gcaacggtaa ttttaaacgt catatatgca caacgaaaaa
    60541 ttttcgtatc tatgccaggg caatttgagt tattaattgt atcaaagtca gtcagtacca
    60601 aagttggagc tgaacccgac ccagaccagg aaataacccg caacgtatcg cctttatacg
    60661 caacagaatc atatttccca tatgcaacct cctttggcgt tatcaccgta acattgaaaa
    60721 cactgctcgc tacagcaccg gttaccttgt ccatggcata ataaactctc acactgtcaa
    60781 aactcgcact cataagttta tctccagcat aactcttaga tgcgccgaca acactcagaa
    60841 cgagcagcat taggctcgcg aaaattatgg tccatttttt catttcagaa atactcaaag
    60901 gttgccgtca ctgtggcact aaagtctccg gacgttacat ctcgcccgct ctttgcctgt
    60961 aaccaggtat taaaatttag gctgttattt ccctgattca gcgggagcgt gacgcctgtt
    61021 gtattgttga ctggcaccga tgtgcctgcc gcagtttcaa taccaatccc agcgccggat
    61081 gctgatgacg aggagtcaaa ggccagaaag ccggatagag cactatcttc tgttcctgtc
    61141 aacgttactt tgacattaaa caatgtagaa ctatgacaat ctttcaattg aaacaccaat
    61201 ggcactcgct ctgactgccc aaggttcatt aaatcgctgg cggcaatggc aggaaaatgt
    61261 acttctgcca actggctacc attaatcact ggcgtacacg atttactgat taaattgccg
    61321 gtaaagtgca aattatctcc tgctattgtg gttccactgc ccagcaacat caggctacta
    61381 ataaaaaaca acgtacgctt tttcttcatt ggtaatctac ctgtaacgtg gcccaggcct
    61441 caaagtccgc ctctggcaga acgacaccac tcttttttac tggcacagcc gttaacaccg
    61501 gtaaatcggt ttcattcacc acaagtggcg tattgatcgt aaaactctgc cctgcctgtt
    61561 tgagctgaat gcctaaacca gtgatattgc tgcttactgc agcattatca aaatcgcttg
    61621 gtgtgccact ccagctcaag gtcatttcca ttaatgggtc gcgaaccgcc gtatcgcagg
    61681 taatggaata cggcacggtt tgctcataat tgctgccatt aatgtcatca ataagcacat
    61741 cctgaaaagt tatttcaatg gtttgagcgt tgctaatcgt gcagttaggc ggctgcacta
    61801 aataaccgtg aaaagagacg tttgagagtg ccatcgagca aaagccccac agcaaaataa
    61861 gcgagatccg tttcattggt aatccaccac aattgtggca ctggcgttaa attcagctgc
    61921 cgctaacgtt gtaccactct gtttcaccgg cacagcttcc agaacgggaa ctcccgagga
    61981 aaaattaaat ggcaaccatg agccagaggt taagtcgagt attgtgtgat cactactttt
    62041 ctgaacccga ataccaaacc caggaatacc cgtgctgata actgtttcac cgttaatcac
    62101 gaccgttgtc gcctgcatct gtagacgcag gtcatccatt gccagattgt tacaaacaag
    62161 cgagtagtca atgggacgtt tataatcaac cccattgatc ttattaatga atacattacc
    62221 aaactcaact gccgcaccat ttaccgtaca aggaggaggg gcatcgacaa gcacccgtaa
    62281 attcatactt actgacttac tcgctgcaac tgcaaagccg ctaccaacac aacatatcag
    62341 taacccagtt accgcccaga cagaacaaga tcctttcata ctggctctcc cggcctcagc
    62401 ctttgcggct ctgctcttca tc
//
LOCUS       DADLSE010000007        61885 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLSE010000007 DADLSE010000000
VERSION     DADLSE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.007"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..758
                     /gene="yegD"
                     /locus_tag="JN692_000441"
     CDS             <1..758
                     /gene="yegD"
                     /locus_tag="JN692_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7262844.1"
                     /translation="DYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLL
                     GHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGIALPILPWWNAVAINDVPAQSDFY
                     SSANGRLLNDLVRDAREPEKVALLQKVWRQRLSYRLVRSAEECKIALSSVAETRASLP
                     FISNELATLISQRGLESALSQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKK
                     ALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(770..2710)
                     /gene="yegI"
                     /locus_tag="JN692_000442"
     CDS             complement(770..2710)
                     /gene="yegI"
                     /locus_tag="JN692_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HAY7262845.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALWSFGIETAAGVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(2707..3468)
                     /locus_tag="JN692_000443"
     CDS             complement(2707..3468)
                     /locus_tag="JN692_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7262846.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLTLNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3465..4124)
                     /locus_tag="JN692_000444"
                     /pseudo
     CDS             complement(3465..4124)
                     /locus_tag="JN692_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(4329..4748)
                     /locus_tag="JN692_000445"
                     /pseudo
     CDS             complement(4329..4748)
                     /locus_tag="JN692_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5066..5707
                     /gene="udk"
                     /locus_tag="JN692_000446"
     CDS             5066..5707
                     /gene="udk"
                     /locus_tag="JN692_000446"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7262847.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5799..6380
                     /gene="dcd"
                     /locus_tag="JN692_000447"
     CDS             5799..6380
                     /gene="dcd"
                     /locus_tag="JN692_000447"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7262848.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            6402..8255
                     /gene="asmA"
                     /locus_tag="JN692_000448"
     CDS             6402..8255
                     /gene="asmA"
                     /locus_tag="JN692_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7262849.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(8707..10290)
                     /locus_tag="JN692_000449"
     CDS             complement(8707..10290)
                     /locus_tag="JN692_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7262850.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFSFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            10949..12088
                     /locus_tag="JN692_000450"
     CDS             10949..12088
                     /locus_tag="JN692_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HAY7262851.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            12094..12537
                     /gene="wzb"
                     /locus_tag="JN692_000451"
     CDS             12094..12537
                     /gene="wzb"
                     /locus_tag="JN692_000451"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HAY7262852.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            12540..14702
                     /gene="wzc"
                     /locus_tag="JN692_000452"
     CDS             12540..14702
                     /gene="wzc"
                     /locus_tag="JN692_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HAY7262853.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHTRPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGGSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            14794..15633
                     /gene="wcaA"
                     /locus_tag="JN692_000453"
     CDS             14794..15633
                     /gene="wcaA"
                     /locus_tag="JN692_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HAY7262854.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEETTQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            15636..16124
                     /gene="wcaB"
                     /locus_tag="JN692_000454"
     CDS             15636..16124
                     /gene="wcaB"
                     /locus_tag="JN692_000454"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HAY7262855.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            16121..17338
                     /gene="wcaC"
                     /locus_tag="JN692_000455"
     CDS             16121..17338
                     /gene="wcaC"
                     /locus_tag="JN692_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HAY7262856.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            17313..18530
                     /gene="wcaD"
                     /locus_tag="JN692_000456"
     CDS             17313..18530
                     /gene="wcaD"
                     /locus_tag="JN692_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HAY7262857.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEVIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFSDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            18541..19287
                     /gene="wcaE"
                     /locus_tag="JN692_000457"
     CDS             18541..19287
                     /gene="wcaE"
                     /locus_tag="JN692_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HAY7262858.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLVHLAQADDISFEWIVVDGGSN
                     DATREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            19303..19851
                     /gene="wcaF"
                     /locus_tag="JN692_000458"
     CDS             19303..19851
                     /gene="wcaF"
                     /locus_tag="JN692_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7262859.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            19877..20998
                     /gene="gmd"
                     /locus_tag="JN692_000459"
     CDS             19877..20998
                     /gene="gmd"
                     /locus_tag="JN692_000459"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7262860.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            21001..21969
                     /gene="fcl"
                     /locus_tag="JN692_000460"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             21001..21969
                     /gene="fcl"
                     /locus_tag="JN692_000460"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7262861.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPASLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            21969..22448
                     /locus_tag="JN692_000461"
     CDS             21969..22448
                     /locus_tag="JN692_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7262862.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            22445..23668
                     /gene="wcaI"
                     /locus_tag="JN692_000462"
     CDS             22445..23668
                     /gene="wcaI"
                     /locus_tag="JN692_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7262863.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            23671..25107
                     /gene="cpsB"
                     /locus_tag="JN692_000463"
     CDS             23671..25107
                     /gene="cpsB"
                     /locus_tag="JN692_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7262864.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLCQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            25300..26670
                     /gene="cpsG"
                     /locus_tag="JN692_000464"
     CDS             25300..26670
                     /gene="cpsG"
                     /locus_tag="JN692_000464"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7262865.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            26725..28119
                     /gene="wcaJ"
                     /locus_tag="JN692_000465"
     CDS             26725..28119
                     /gene="wcaJ"
                     /locus_tag="JN692_000465"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7262866.1"
                     /translation="MINLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            28121..29599
                     /gene="wzxC"
                     /locus_tag="JN692_000466"
     CDS             28121..29599
                     /gene="wzxC"
                     /locus_tag="JN692_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7262867.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIIHYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            29779..31059
                     /gene="wcaK"
                     /locus_tag="JN692_000467"
     CDS             29779..31059
                     /gene="wcaK"
                     /locus_tag="JN692_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7262868.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            31056..32276
                     /gene="wcaL"
                     /locus_tag="JN692_000468"
     CDS             31056..32276
                     /gene="wcaL"
                     /locus_tag="JN692_000468"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7262869.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            32287..33681
                     /gene="wcaM"
                     /locus_tag="JN692_000469"
     CDS             32287..33681
                     /gene="wcaM"
                     /locus_tag="JN692_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7262870.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            33856..34749
                     /gene="galF"
                     /locus_tag="JN692_000470"
     CDS             33856..34749
                     /gene="galF"
                     /locus_tag="JN692_000470"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7262871.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            35122..36207
                     /gene="rfbB"
                     /locus_tag="JN692_000471"
     CDS             35122..36207
                     /gene="rfbB"
                     /locus_tag="JN692_000471"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7262872.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHAGICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            36207..37106
                     /gene="rfbD"
                     /locus_tag="JN692_000472"
     CDS             36207..37106
                     /gene="rfbD"
                     /locus_tag="JN692_000472"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7262873.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            37165..38043
                     /gene="rfbA"
                     /locus_tag="JN692_000473"
     CDS             37165..38043
                     /gene="rfbA"
                     /locus_tag="JN692_000473"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7262874.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            38048..38593
                     /gene="rfbC"
                     /locus_tag="JN692_000474"
     CDS             38048..38593
                     /gene="rfbC"
                     /locus_tag="JN692_000474"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7262875.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWQVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            38609..39865
                     /gene="wzx"
                     /locus_tag="JN692_000475"
     CDS             38609..39865
                     /gene="wzx"
                     /locus_tag="JN692_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7262876.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            39895..40785
                     /locus_tag="JN692_000476"
     CDS             39895..40785
                     /locus_tag="JN692_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7262877.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNSTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGDGRYNA"
     gene            40814..41683
                     /locus_tag="JN692_000477"
     CDS             40814..41683
                     /locus_tag="JN692_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7262878.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            41703..42851
                     /gene="wzy"
                     /locus_tag="JN692_000478"
     CDS             41703..42851
                     /gene="wzy"
                     /locus_tag="JN692_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7262879.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSAKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            42855..43229
                     /locus_tag="JN692_000479"
     CDS             42855..43229
                     /locus_tag="JN692_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HAY7262880.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            43292..44218
                     /locus_tag="JN692_000480"
                     /pseudo
     CDS             43292..44218
                     /locus_tag="JN692_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067396.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            44403..45002
                     /locus_tag="JN692_000481"
     CDS             44403..45002
                     /locus_tag="JN692_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262881.1"
                     /translation="MSLSILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFAVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYLGIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            45103..45654
                     /locus_tag="JN692_000482"
     CDS             45103..45654
                     /locus_tag="JN692_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262882.1"
                     /translation="MIAKKRNIQTFLIFSLATILGGIYWYVRSFWISGDPFSPAGGNI
                     FGHYLWNEIDLQVQTAEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKIL
                     WVFFSIVLTYVLFWFSVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFI
                     LVCIIFFRGDRCILNSNIFILNT"
     gene            45651..46010
                     /locus_tag="JN692_000483"
     CDS             45651..46010
                     /locus_tag="JN692_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262883.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIFISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            46119..47525
                     /gene="gndA"
                     /locus_tag="JN692_000484"
     CDS             46119..47525
                     /gene="gndA"
                     /locus_tag="JN692_000484"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7262884.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            47775..48941
                     /gene="ugd"
                     /locus_tag="JN692_000485"
     CDS             47775..48941
                     /gene="ugd"
                     /locus_tag="JN692_000485"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7262885.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            49087..50064
                     /gene="wzzB"
                     /locus_tag="JN692_000486"
     CDS             49087..50064
                     /gene="wzzB"
                     /locus_tag="JN692_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7262886.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(50250..50861)
                     /locus_tag="JN692_000487"
     CDS             complement(50250..50861)
                     /locus_tag="JN692_000487"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7262887.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANSIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(50855..51631)
                     /gene="hisF"
                     /locus_tag="JN692_000488"
     CDS             complement(50855..51631)
                     /gene="hisF"
                     /locus_tag="JN692_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7262888.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            complement(51613..52350)
                     /gene="hisA"
                     /locus_tag="JN692_000489"
     CDS             complement(51613..52350)
                     /gene="hisA"
                     /locus_tag="JN692_000489"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7262889.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            complement(52350..52940)
                     /gene="hisH"
                     /locus_tag="JN692_000490"
     CDS             complement(52350..52940)
                     /gene="hisH"
                     /locus_tag="JN692_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7262890.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRHSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            complement(52940..54007)
                     /gene="hisB"
                     /locus_tag="JN692_000491"
     CDS             complement(52940..54007)
                     /gene="hisB"
                     /locus_tag="JN692_000491"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7262891.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            complement(54007..55077)
                     /gene="hisC"
                     /locus_tag="JN692_000492"
     CDS             complement(54007..55077)
                     /gene="hisC"
                     /locus_tag="JN692_000492"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7262892.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRPLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            complement(55074..56378)
                     /gene="hisD"
                     /locus_tag="JN692_000493"
     CDS             complement(55074..56378)
                     /gene="hisD"
                     /locus_tag="JN692_000493"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7262893.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLIRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYVAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            complement(56384..57283)
                     /gene="hisG"
                     /locus_tag="JN692_000494"
     CDS             complement(56384..57283)
                     /gene="hisG"
                     /locus_tag="JN692_000494"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7262894.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            complement(57429..57479)
                     /gene="hisL"
                     /locus_tag="JN692_000495"
     CDS             complement(57429..57479)
                     /gene="hisL"
                     /locus_tag="JN692_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7262895.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            57762..58013
                     /gene="yefM"
                     /locus_tag="JN692_000496"
     CDS             57762..58013
                     /gene="yefM"
                     /locus_tag="JN692_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7262896.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            58010..58264
                     /gene="yoeB"
                     /locus_tag="JN692_000497"
     CDS             58010..58264
                     /gene="yoeB"
                     /locus_tag="JN692_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7262897.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            58347..59171
                     /locus_tag="JN692_000498"
     CDS             58347..59171
                     /locus_tag="JN692_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7262898.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHT
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGRIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            59217..60146
                     /locus_tag="JN692_000499"
     CDS             59217..60146
                     /locus_tag="JN692_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262899.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            60361..60423
                     /gene="yoeI"
                     /locus_tag="JN692_000500"
     CDS             60361..60423
                     /gene="yoeI"
                     /locus_tag="JN692_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7262900.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            60413..61771
                     /gene="plaP"
                     /locus_tag="JN692_000501"
     CDS             60413..61771
                     /gene="plaP"
                     /locus_tag="JN692_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7262901.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIVLVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWICEKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
BASE COUNT        15753 a        14527 c        15721 g        15884 t
ORIGIN      
        1 tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat atcggcggtg
       61 gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg
      121 aagccagtct gctgggtcac agtggttgtc gtattggcgg taacgatctg gatatcgccc
      181 tggcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg
      241 ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa cgacgttcct gcacagagtg
      301 atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac
      361 cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc
      421 gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc gcctcactgc
      481 cgtttatcag caatgaactg gctacgctga ttagccagcg agggctggaa agcgccctca
      541 gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac gcccaggaaa
      601 aaccggacgt tatctacctg accggcggta gcgcccgttc tccgctgatt aaaaaagcgc
      661 tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc tccgtcaccg
      721 ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa gaaaatggct cagcagcggc
      781 ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg acgtaacggt tccatcaacg
      841 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
      901 acgtcgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgcc tgtctgtctg
      961 ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
     1021 cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacg ccacgacggg
     1081 taacacctgc tgctgtttca ataccaaaag accataacgc cgctttacgc gcagggccaa
     1141 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt
     1201 cccttgcagt gtcatgaagt gctgccagag cgagtttctc ttcttccggt aacccgagaa
     1261 tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc aggccgccta
     1321 cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta aatgcctcac
     1381 ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag
     1441 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
     1501 gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
     1561 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc aatgctggcg
     1621 gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc ccgccggtgg
     1681 taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgtacacc
     1741 acgggcagtc ggtcaaatga gcgggataaa catgcattgc cgaaacggga catttcttta
     1801 actgttggcg cagagaatca agtgccgcta cccacgcttt agccgtcgga cgcccggttg
     1861 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcactc ggtaacatcg
     1921 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
     1981 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
     2041 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
     2101 aattatcgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
     2161 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
     2221 gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct
     2281 ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
     2341 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
     2401 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
     2461 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
     2521 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
     2581 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
     2641 caccgccttt gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag
     2701 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
     2761 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
     2821 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc
     2881 caacatattt aacgtcagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
     2941 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaagtgcgt
     3001 catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt gaccaaaatc
     3061 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg atgaaatcaa
     3121 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
     3181 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattaa gccccaattc
     3241 cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga gcatcgcgcc
     3301 ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggctg
     3361 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcaaggca agtccgcgct
     3421 gatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
     3481 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
     3541 gaactggata atcagctaaa cagttcacgg aattgtaatc cttgcagagg caaaggctga
     3601 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaaa
     3661 gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg
     3721 gttggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca
     3781 cgatactcac gtttccgctc ttctaccata tccagggctt tggtaatggc cgcacccatt
     3841 ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt
     3901 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
     3961 gaatcggcaa gcagctcatc gcgaaaggta accaatccgg tgttaagttc attaatagtt
     4021 ctgccactca tcgagccaga aacatccagc agcagaatgc aggggcaacg tggttccggg
     4081 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
     4141 gagaattatt gaggtccggt atcataccga ctgcaataat gaaataagag caaacgaaga
     4201 aactttcaga attagtcccg gcggcgcaga aaattttgct attcgcctcg caagaacgga
     4261 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
     4321 ggtttccccc cccccaacag aggcggaaca atggcgctgc cgaatgccag acgcagccag
     4381 tcagccagat tttgcaaggg gttataccac ggcagcaatt tacgtagcag cactgccccg
     4441 accacggctt caacaatatt gatggtcgtc caggtcatgt tcagcgagct ggtggaaaaa
     4501 agcaggatgg atgcggcgat atttcccagc gaacagctca gcgcaattcc cggcaacatg
     4561 cgcccggcat ggcgataaaa cgccaccatc atgatggacg tcgggaacca cagtggggcg
     4621 agttgggtgc caaactgcga aagctcaagc gagacaaggg taaagataaa cgagactaaa
     4681 cctaaactga ccaggtgaag cagcgggtgg ggcagggcaa ttaatacatg ctgtgattgt
     4741 ttgcttatta ccgctttatc cgcaaggcca ggtcgccagt attcgtttct gtgtttatgc
     4801 tagtacaaaa gttttacatt ttatatgcga attgctcata aagtgacgta aaggcggata
     4861 atttgcgcaa ctgcgtataa cattttttac cttacataaa actgatcaac gtaatttgcc
     4921 accgggaatt ctttgtcaac aattaactct gagcgcgcgg caacgctatt cgactggtat
     4981 cagacggatg aaatccctat aattgccgcg tttggcgctt cgtcgccccc ttcctaacat
     5041 ccaggttaat caggtcgcta aatatatgac tgatcagtct catcagtgcg tcattatcgg
     5101 tatcgctggc gcatcggctt ccggcaagag tcttattgcc agtacccttt atcgtgaatt
     5161 acgtgagcaa gtcggtgatg aacacatcgg cgtaattccc gaagactgct attacaaaga
     5221 tcaaagccat ctgtcgatgg aagaacgcgt taagaccaac tacgaccatc ccagcgcgat
     5281 ggatcacagc ctgttgcttg agcatttaca agcgttgaaa cgcggctcgg caattgacct
     5341 gccggtttac agctatgttg aacatacgcg tatgaaagaa acggtgacgg ttgagccgaa
     5401 gaaggtcatc attctcgaag gcattttgtt gctgacggat gcgcgtttgc gtgacgaact
     5461 taacttctcc attttcgttg ataccccgct ggatatctgc ctgatgcgcc gcatcaagcg
     5521 tgacgttaac gagcgtgggc gttcaatgga ttccgtgatg gcgcaatatc aaaaaaccgt
     5581 gcgcccgatg ttcctgcaat tcattgagcc ttctaaacaa tatgcggaca ttatcgtgcc
     5641 gcgcggcggg aaaaaccgca tcgcgatcga tatattgaaa gcgaaaataa gtcagttctt
     5701 tgaataagct tgataaattg tgtaccgttc agtgataagc tggtatgccc ttgacgtaat
     5761 tggcgttaag ggagtgatgc gcgaaaggag aaaatgccat gcgtctgtgt gaccgagata
     5821 ttgaagcctg gcttgatgaa ggccgtttgt cgatcaaccc acgtccgcca gtggagcgta
     5881 ttaacggcgc gacggtggat gtacgcctgg gcaataaatt tcgtaccttc cgtggtcaca
     5941 cggcagcgtt tatcgatctg agcggtccca aagatgaagt gagcgccgcg cttgaccgcg
     6001 tgatgagcga tgagatcgtt ctcgacgagg gcgaggcgtt ctatcttcac ccaggagagc
     6061 tggcgctggc ggtgacgctg gagtcggtga cgctgccagc cgatctggtg ggctggctgg
     6121 acgggcgttc ctctttggcg cgtctggggc tgatggtgca cgtcaccgcg caccgtatcg
     6181 atccgggctg gtctggttgc attgtgctgg agttctacaa ctccggtaag ctgccgctgg
     6241 cgctgcgtcc gggcatgtta attggtgcgc tgagctttga gccgctttcc ggcccggcgg
     6301 cgcgacctta caaccgccgt gaagatgcga aatatcgcaa ccagcagggc gcggtagcca
     6361 gccgaatcga taaagactaa ttcaggccca ttgaggatac catgagacga tttctgacga
     6421 cgctgatgat actcctggtc gtgctggtgg ccgggttatc tgcgttagtg ttgctggtga
     6481 atccgaatga tttccgcgac tatatggtca agcaagttgc tgcacgtagc ggttatcaat
     6541 tgcagctcga cgggccactg cgttggcacg tctggccgca gcttagtatc ctctccgggc
     6601 gaatgtctct caccgcccag ggcgcaagcc agccactggt tcgcgccgac aacatgcgtc
     6661 tggacgtggc gcttttacca ctactgagtc atcaactgag cgttaagcag gtgatgctaa
     6721 aaggggcagt gatccaactg acgccgcaga cggaagcggt gcgcagtgaa gacgctccgg
     6781 ttgcaccgcg cgacaatacc ttgccggatc tgtcagacga tcgcggatgg tcgtttgata
     6841 tatccagtct taaggtggcg gacagcgttc tggtgttcca gcatgaagat gacgagcagg
     6901 tgacaatccg caatatccgc ctgcaaatgg aacaagatcc ccaacatcgt ggctcatttg
     6961 agttctccgg gcgggttaat cgcgatcagc gcgatctcac gatatccctt aacggtacgg
     7021 tagatgcttc tgattatccg catgatttaa cagcggctat tgaacaaatt aactggcagt
     7081 tgcagggtgc cgatttacca aaacaaggta ttcaggggca ggggagtttc cagtcccagt
     7141 ggcaggagtc acataaacgc ctttcattta accaaattag tttgaccgcc aatgacagta
     7201 cgctgagcgg gcaagcacag gtcacgctga cagagaaacc ggaatggcag ctgagactgc
     7261 aattcccgca actgaatctt gacaacctca tcccgcttaa tgaaaccgcg aatggtgaaa
     7321 acggtgccgc gcagcagggg cagagccaat caacgttgcc gcgcccggtc atttcttcgc
     7381 gtattgatga accggcctat cagggactgc aaggctttac agctgatatt ttgttgcagg
     7441 ccagtaacgt gcgctggcgc ggaatgaatt ttacagatgt tgccacgcaa atgaccaaca
     7501 agtcgggttt gctggaaatt actcaactgc agggcaagct taacggtgga aaggtttcac
     7561 tgccgggcac gctggacgcg acatcaataa atccgcggat aaacttccag ccacggctgg
     7621 agaacgttga gattggtacc attctgaagg cgtttaacta tccgatttcg ttgaccggaa
     7681 aaatgtcact ggctggtgat ttctccggtg ctgacataga tgccgacgca ttccgccaca
     7741 actggcaagg gcaggcgcat gttgagatga ccgacacacg tatggaaggg atgaacttcc
     7801 agcagatgat tcagcaagcg gtagagcgta atggcggtga tgtgaaggcc gctgaaaact
     7861 tcgataacgt gacgcggctt gaccgcttta ccaccgattt gacgttgaag gatggcgtcg
     7921 tgacgttaaa cgacatgcaa ggtcaatcgc ctgtgctggc gctgacaggg gaaggcatgt
     7981 tgaatctggc agatcaaacc tgcgacaccc agtttgatat tcgggtcgtg ggtggctgga
     8041 acggggaaag caaactgatt gatttcctca aagaaacgcc agtaccgctg cgggtttatg
     8101 gcaactggca gcaactcaat tacagcctgc aagtggatca gttactgcgc aaacatctac
     8161 aggacgaagc gaaacgtcgc ctgaatgact gggccgagcg gaataaagat tcccgcaatg
     8221 gcaaagatgt gaagaagttg ctggagaaga tgtaggttac caggtgacct gtcctgtagg
     8281 cctgataaga cgcgtaaagc gtcgcatcag gcgttcgcgg cggcactatt atattctgaa
     8341 tatgtaagat cgaaggctgt tccgttcact tgatgttcct cgctactatg gaacgctcaa
     8401 gccggtgaac gtgccagggt ggctcaatcg ccaccaccct ggcaacccgg gctcccggcg
     8461 gtaaatcgcc gctggcgcgg ttccctcatc ttattccttt cggctatcgg gtacgggcgc
     8521 gaggtaacat ccctgtaaaa cgcgccctca gcccacatcc atgtgggctg ccccagccta
     8581 caggaaacga ttcggcgatt tacagccgga cccacaccac cgctgtgatg tccgtttacc
     8641 acagacaacg cttcattgct gaagttataa ggcgttacgc agcattgggt aaaatggccg
     8701 ataccatcac acctcgtact ccatctcgcc atccttacgc agcggtataa tctgcacctt
     8761 ctgcacgcga tggctttcca cctgcaacgt tttaagcaaa taatccccca cctgaacttc
     8821 ttcgccaggt tttggaatac gctgcaaata ctccatcaac agcccggcaa tggtgtgata
     8881 ttcacgtttt tcatccagcg gcagcggcac atattgcacc agatcctcca gcggcatatg
     8941 accattcgcc gtccaggaac cgtccgcatt cttctgaata tcatggcggg cgtcgatctc
     9001 ttccacttcg ttcggtaagt taccggcaat ggtttcagtg acgtcactta atgtcacaat
     9061 cccttccacc gagccaaact catccaccac aaaagcaaag tgcgtgcggg cattacggaa
     9121 ctgctccagg gctggtaaca acggcaaggt ttccgggaac accagcggct ggcgaatcaa
     9181 cacccgcagg ttgagcggtt cgccgcgcag tgactgttgt agcaggtcga taacgtgaac
     9241 aacaccgagc aaatcttctg cgtcatcacc gtcggtaacc accagccgcg tatgctgatt
     9301 tcgctccagc aactggcgga tttcttcttc cggcgcgttg agatcgatat gctcaatatc
     9361 gtggcgcgac gtcataatgc tgctgacggt acgctggtta agattaagta cccgctcaat
     9421 catccgccgt tcctgcggat taaagatctg ctggtttcca tgatccacca gcatggacgc
     9481 ggtttcggcg tccagttccg catcttcttt ttgcccgcta agcaggcgca ttaccgcttc
     9541 ggtagtccgc tggcgtagcg tctgattagc ggaaagaaaa cgtcgccggt taaagatagc
     9601 caactgattg agtgcctcga tcatcactga gaaaccaatg gcagcgtaca ggtagccttt
     9661 cggaatgacg aagctgaaac cttctgccac caggctaaag ccaatcatca acaggaagct
     9721 taagcagaga ataacgatgg tcgggtgact gttaacaaat tgcgttaacg gcttgctggc
     9781 catcaacatc aggctgatag cgataaccac ggcggccatc atgaccagta aatggtcgac
     9841 catcccgacg gcggtaatca ctgagtcgag tgagaaaatg gcgtccagta ccacaatttg
     9901 cgtcaccacg ccccagaact tcgcaccttt gcgttgtgtg ggattattgc tgtctttccc
     9961 ttccagccgt tcgttcagct ccatcgtggc tttgaacaac aggaagaaac caccaaacag
    10021 catgattaag tcgcgggcgc taaaggtaaa cgagcggaag ctgaacagcg gttgagtcag
    10081 ggtgaccagc caggagattg acgccagcag taacaggcgc attaacatcg ccagcagcag
    10141 cccggtaacc cgtgcgcggt cgcgctgctt cggcggtagt ttttcggcga ggatggcaat
    10201 aaagaccagg ttatcaatgc cgaggaccag ttcgatcaca atcagcgtga ttaacccggc
    10261 ccagatagac ggatcggcaa tccattccat agtaagtaca aaacctattc gttgtatgac
    10321 aaatgtcaca attcgatcat ggataaatcg gggcaaaaat gcaatgttgc agcactattt
    10381 atctcatatt atttgagggt aataacctca cattatccct gaattaaaag cggtaataat
    10441 aaagcattat ttaagaaata tcgcatatgg agacgcaata ttaaatattg tcaacctaaa
    10501 gaaactccta aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc
    10561 cgaattgtta ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag
    10621 agccaatatt caattaccat aatgtcacct tatattcgga agttaatttc cgcaataacc
    10681 ctttctcatc aacgattgta tgcagagagg gtttgaaaaa ttaagctcaa tcagtgtatt
    10741 ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgaaagacca gtctgaatta
    10801 ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaattttt
    10861 attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata
    10921 attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt
    10981 attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac
    11041 gatgggcaaa gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa
    11101 tgtttatccg ctgaccccgc gcctgattga ccaattacgc ccacgcccga atgtagcgcg
    11161 ccccaatatg acgctggaaa gcgagatcgc gaattaccag tatcgcgtcg ggccggggga
    11221 tgttcttaat gtcaccgtct gggatcaccc ggaactcacc acgccagctg gtcagtaccg
    11281 cagctccagc gacaccggca actgggtaca gcctgacggc actatgtttt acccgtatat
    11341 cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg
    11401 cttagcgacg tacatcgctg acccgcaggt ggacgttaat atcgccgcct tccgctcaca
    11461 aaaggcctat atctcaggtc aggtgaataa atccggtcaa caggcgatca ccaacgtacc
    11521 actgaccatt ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg
    11581 caacgtggtg ctaacacaca atggtcgtga agaacgcatt tctttacagg cgctgatgca
    11641 aaacggcgac ctcaatcaga accgactgct ttatcccggc gatattctct acgtgccgcg
    11701 taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat
    11761 ggactttagc ggcatgaccc tgactgaagc tctgggcaat gccgaaggta tcgacatgac
    11821 cacctccaac gccagcggca tctttgtcat tcgtccgctg aaaggcgagg gcgggcgtaa
    11881 cggcaagatt gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtgatggc
    11941 gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtttcccg
    12001 ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga
    12061 tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt
    12121 gtcggcaata tttgccgttc cccgatggcg gaacgcttac tgcaacgtta tcacccggaa
    12181 ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta agggcgctga tcctaccgct
    12241 atcagcgtcg ccgcagaaca tcaactgtct ctggaaggcc actgtgcacg acaaatcagc
    12301 cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg ataagcgcca tatcgaacgc
    12361 ttatgcgaga tggcaccgga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac
    12421 gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca
    12481 ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa
    12541 tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg
    12601 gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt gattggcatc accgccgtat
    12661 ttgccctctg tgccgtggtt tacactttct tcgccacgcc gatttatagt gccgacgcac
    12721 tggtacaaat cgagcaaaac agcggcaatt cgctggtgca ggatatcggc tcggcattag
    12781 ccaacaaacc gcctgcatcg gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg
    12841 gtaaaacggt ggacgatctc gacctcgata ttgcggtgag caaaaacacg ttcccgattt
    12901 tcggggcggg ctgggatcgc ctgatggggc gtcagaacga gacggtgaaa gtgactacct
    12961 ttaaccgccc gaaagagatg gcggatcagg tgtttacgct taatgtgctg gatgacaaaa
    13021 actacaccct gagcagtgat ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga
    13081 aaaaagaagg cgtcacgctg atggttgaag ccattcacac ccgcccgggc agtgagttta
    13141 ccgtcaccaa atactccacg ttggggatga tcaaccaact gcaaaacagc ctgacggtaa
    13201 cggagaacgg caaagacgca ggcgtactga gcctgactta taccggtgaa gatcgcgaac
    13261 agatccgcga cattcttaac agcatcgctc gtaactatca ggaacaaaat attgagcgca
    13321 aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta
    13381 gccgcctcga tgttgccgaa aacaaactga atgccttccg tcaggataaa gattctgttg
    13441 atctgccgct tgaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga
    13501 acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt cacccggcgt
    13561 accgcacgct gctggagaaa cgtcaggcgc tggaagaaga gaaagccaaa cttaacggtc
    13621 gcgtaacggc gatgccgaaa acccagcagg aaattgtccg tctgacccgc gatgtcgagt
    13681 ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag
    13741 ccagcaccgt cggcgatgtg cgcattattg acccggcaat cacccagcct ggcgtgctaa
    13801 aaccgaagaa agggctgatt atcctcggcg cgattatcct tggcctgatg ctctctatcg
    13861 tgggtgtgct gctgcgctcg ttgtttaatc gcggcatcga aagcccgcaa gtgctggaag
    13921 aacacggaat cagcgtctat gccagcatcc cgctgtcgga gtggcagaaa gcgcgcgata
    13981 gcgtcaaaac catcaaaggg gttaaacgct ataaacagag ccagctactg gcagtgggga
    14041 acccaaccga tctggcgatt gaagccatcc gtagtctgcg taccagtctg cacttcgcga
    14101 tgatgcaggc gcagaacaat gtgttgatga tgaccggggg tagcccgtca atcggtaaaa
    14161 cctttgtctg cgccaacctg gcggcggtga tcagccagac caataaacgc gtgttgttga
    14221 tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat aatgtcaatg
    14281 ggctgtcgga gattctgatt ggtcagggcg atattactac cgctgctaaa ccgacctcta
    14341 ttgccaaatt tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct gaactgttaa
    14401 tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa aaactatgac ctggtgttga
    14461 ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgtcggaa
    14521 ccacgttaat ggtggcgcgt tatgcggtca acacattgaa agaagtggaa accagtctga
    14581 gccgctttga gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc
    14641 gcgccagcgc gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat
    14701 aacgacgcct gcattcgcac cgccccgtag gccggataag gcgctccgcc gcatccggca
    14761 agcaaaccag ctcataagcc gggagaacaa cctatgaaag acaatccgct gatctcaatc
    14821 tatatgccaa cctggaaccg ccaacaactg gcgattcggg cgataaaatc ggtcctgcgc
    14881 caggactaca gcaactggga gatgatcatc gtggatgatt gctccacctc ctgggaacaa
    14941 ctgcaacagt acgtcactgc cctcaacgat ccgcgtatca cttacatcca caacgacatt
    15001 aacagcgggg cgtgcgcggt gcgtaaccag gcgattatgc tggcgcaagg ggaatacatc
    15061 accgggatcg atgatgatga tgaatggacg cccaaccgcc tgagcgtctt cctcgcccat
    15121 aaacagcaac tggtcacaca cgcctttttg tacgctaacg actacgtttg ccagggagaa
    15181 gtttattccc aaccggcgag cttaccactg tatcccaaat cgccgtactc acgccgcctg
    15241 ttctataaac gcaatatcat tggcaatcag gtctttacct gggcatggcg tttcaaagag
    15301 tgtttgttcg ataccgaact gaaagcagca caggattacg acatattcct gcggatggtg
    15361 gtggagtacg gcgaaccgtg gaaagtggaa gagacgacgc agatcctgca tatcaatcac
    15421 ggcgaaatgc agatcacctc gtcaccgaaa aaattctccg gatacttcca cttttaccgc
    15481 aagcacaaag acaaattcga ccgcgccagt aaaaaatatc agctgtttac cctctaccag
    15541 atccgcaata aacgtatgac ctggcgcacc ttgttgacgc tgctgtcggt gcgtaacggc
    15601 aaacgtctgg ctgatggtat tcgggggcgc taaagatgct ggaagatctg cgcgccaaca
    15661 gctggagttt acgcccgtgc tgcatggtac ttgcctatcg cgtcgctcat ttttgttcgg
    15721 tgtggcgcaa aaagaacgtc ctcaacaatc tgtgggcggc cccgctgctg gtgctgtatc
    15781 gcattatcac cgaatgcttt ttcggttatg aaatccaggc cgccgcgacc attggccgcc
    15841 gctttactat ccatcacggt tacgccgtag tgatcaataa aaacgtggtg gcgggggatg
    15901 atttcaccat tcgccacggc gtcactatcg gcaatcgggg tgccgataac atggcatgtc
    15961 cacacattgg caacggcgtc gaactcggtg ccaacgtcat tattcttggt gatatcacgc
    16021 ttggtaacaa cgtcaccgtg ggtgcgggca gcgtggtgct cgactctgtc ccggacaacg
    16081 cgctggtggt gggagaaaaa gcgcgagtga aggtaattaa atgaatattc tgcaatttaa
    16141 tgtgcgactg gcggaaggcg gggcagcagg tgtggcgtta gatctccacc agcgtgcgct
    16201 gcaacagggg ctggcgtcac attttgtcta cggctacggc aaaggcggca aagagagcgt
    16261 cagccatcag aactatccgc aggtcatcaa acatacgccg cggatgaccg cgatggcgaa
    16321 cattgcgttg tttcgtctgt ttaatcgcga tctgttcggc aatttcaatg agttatatcg
    16381 caccattact cgcacaccgg gtccggtggt cctgcatttt catgtgctgc acagctactg
    16441 gttgaatctt aacagcgtgg tgcgcttttg cgaaaaagtg aaaaaccata aaccggatgt
    16501 caccctggtc tggacgctgc acgaccactg gagtgtaacc ggacgctgcg cctttaccga
    16561 cggttgcgaa ggctggaaaa cgggctgcca gaaatgcccg accttaaata actatccgcc
    16621 ggtgaagatt gatcgcgcgc accagctggt ggcgggcaaa cgccagttat tccgcgagat
    16681 gctggcgctg ggctgtcagt ttatttcccc cagccagcat gtggctgacg ctttcaatag
    16741 tctgtacggg ccagggcgtt gccggattat caataatggc attgatatgg caaccgaagc
    16801 gattctggca gatctgcctc cggtgcgcga aactcagggc aagccgaaaa tcgcggtggt
    16861 ggcgcatgac ctgcgttacg acggcaaaac taaccagcaa ctggtgcgtg agatgatggc
    16921 gctgggcgac aaaatcgaac tgcatacctt tggtaagttc tcgccgttca ccgctggcaa
    16981 cgtggttaat cacggctttg aaactgacaa gcgcaagttg atgagcgcgc tcaatcagat
    17041 ggatgcgctg gtgttcagtt ctcgtgtcga taactacccg ctgattttgt gtgaggcgct
    17101 atcgattggc gtgccggtga ttgccaccca tagcgatgcg gcgcgggaag tgctgcaaaa
    17161 atccggtggt aaaaccgtca gtgaagaaga ggtgctgcaa ctggtacagt taagcaaacc
    17221 ggaaatcgcg caggcgatat ttggtaccac gctggctgag ttcagccaac gcagtcgcgc
    17281 cgcctacagt ggacaacaga tgctggagga gtatgtcaac ttctatcaga atctgtagct
    17341 acctgctgct gccactgatc tacctgctgg ttaacgtcaa aatcgcccag cttggcgaaa
    17401 gtttccccat caccatcgtc acttttttac ctgtcttgtt actgctgttt ttagaacgca
    17461 tcagcattaa aaaattgatg attgccttag ggattggcgc gggactcacg gcgtttaact
    17521 acctgtttgg tcagtcgttg gatgccggta aatacgtcac ttcaactatg ctgtttgtct
    17581 atattgtgat cattattggc atggtgtgga gtattcgttt taaaacaatt tcgccacaca
    17641 accatcgtaa gatattacgt ttcttttatc tggtggtcgg actggtggtg gcgctggcgg
    17701 cggtggagat ggcgcaaatt atcctcaccg gtggcagcag tattatggag tcgatttcga
    17761 aatatctgat ttacagcaac agctatgtgc tgaatttcat taaattcggc ggcaagcgca
    17821 cgacagcact ttatttcgaa ccggcatttt tcgctctggc attaatctca atttggctca
    17881 gcatcaaaca gtttggtatc aaaacgccta aaacagatgc tatgattctc gcagggataa
    17941 tattatccgg atcgttttca ggggttatga cctttatcct gttttatttg ctggagtggg
    18001 catttcaata tctgaataaa gaggtgatta agaaaaaatt accgttagca ttgatttctc
    18061 tggctgtatt cctggttggt gtggtaatcg cgtttcctta tatttccacc cgtctgggcg
    18121 atttaggtac ggaaggatcg tcatcgtatt atcgtattgt cggtccgctg gtgatggtcg
    18181 gttattcttt gacccatatt gacggtgtag tcagatttgg ctcactttat gaatatgttg
    18241 catcattcgg aatatttaac ggtgcggatg tcggaaaaac catagacaat ggtttgtatc
    18301 tgctgattat ttatttttcc tggttcgcgg tgtttttatc gctgtggtac atggggaaag
    18361 tgataaaaat gatgatcaac gcttttagtg ataaccgcaa ttttcgcgtg caattatatc
    18421 tttttactcc ggtatcgctg ttttttaccg gttcgatatt tagcccggaa tatgcatttt
    18481 taatcgtctg tccgtttatt ttgcgaaaag cgttaaatat tacgaggtaa gaataagaac
    18541 atgttgctta gcataatcac tgtcgcgttt cgtaacctcg aagggatagt caaaacacat
    18601 gcctcgctgg tgcatctggc gcaggcggac gatatcagct tcgaatggat tgttgtcgat
    18661 ggcggttcca acgacgctac ccgtgagtat ctggaaaatc tcaatggtat ctataaccta
    18721 cgctttgtca gcgagccaga taacggtatc tacgacgcca tgaacaaggg cattgcgatg
    18781 gcacaaggca agttcgcgtt gtttctcaac tcgggcgata tttttcatca ggatgccgca
    18841 aattttgtcc gtaagttaaa aatgcaaaaa gataacgtga tgatcactgg cgatgcgctg
    18901 ctggattttg gcgacgggca taaaattaaa cgtagcgcca aaccgggctg gtatatttat
    18961 cacagcctgc ccgccagcca tcaggcgata tttttcccgg tatccggctt gaaaaaatgg
    19021 cgttatgacc tggaatataa agtttcttct gattacgcgc tggcagctaa aatgtataaa
    19081 gccggttatg catttaaaaa actcaatggc ctggtgtctg aattttctat gggtggggta
    19141 tcaaccacca ataatatgga attgtgtgct gacgccaaaa aagtccaacg gcagatatta
    19201 catgtacctg gcttctgggc agaattatcc tggcatttac gccaacgtac gacaggtaag
    19261 actaaagcct tatataacaa agtctgaata taaggaaaac caatgcaaga tttaagcggt
    19321 ttctcggtgc cgaaagggtt ccggggcggc aacgctatta aagtgcaatt atggtgggca
    19381 gtacaggcaa caatatttgc ctggtcgcca caagtattgt atcgctggcg ggctttttta
    19441 ttacgtttat tcggagcaaa aataggaaaa aacgtagtta ttcgtccgtc agtaaaaatt
    19501 acctatccgt ggaaattaac cttaggtgat tacgcgtggg tcggcgatga cgtcaattta
    19561 tataccctcg gtgaaataac cattggcgca cattcggtga tatcgcaaaa aagttattta
    19621 tgcaccggta gccacgacca tgcaagtcaa catttcacca ttaacgccac gcctattgtg
    19681 attggcgaga aatgctggct ggcaaccgat gtctttgttg ccccaggcgt cacaatcggc
    19741 gacggcaccg tcgtgggtgc acgaagcagt gtttttaaat cgcttccggc aaatgtggtt
    19801 tgccgaggga atcccgcagt ggtgatacgc gaacgcgttg aaactgaata aattcaaaaa
    19861 tacagaggaa taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg
    19921 ttcttacctg gcagagtttc tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg
    19981 cgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatccgc acacctgcaa
    20041 cccgaaattc catctgcatt atggcgacct gagtgatacc tctaacctga cgcgcatttt
    20101 gcgtgaagta cagccggatg aagtgtacaa cctgggcgca atgagccacg ttgcggtctc
    20161 ttttgagtca ccagaatata ccgctgacgt cgacgcgatg ggtacgctgc gcctgctgga
    20221 ggcgatccgc ttcctcggtc tggaaaagaa aactcgtttc tatcaggctt ccacctctga
    20281 actgtatggt ctggtgcagg aaattccgca gaaagaaacc acgccgttct acccgcgatc
    20341 tccgtatgcg gtcgccaaac tgtacgccta ctggatcacc gttaactacc gcgaatccta
    20401 cggcatgtac gcttgtaacg gaattctctt caaccatgaa tccccgcgcc gcggcgaaac
    20461 cttcgttacc cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg
    20521 cctgtacctc ggcaatatgg attccctgcg tgactggggc cacgccaaag actacgtaaa
    20581 aatgcagtgg atgatgctgc aacaggaaca gccggaagat ttcgttatcg cgaccggcgt
    20641 tcagtactcc gtacgtcagt tcgtggaaat ggcggcagca cagctgggca tcaaactgcg
    20701 ctttgaaggc acgggtgttg aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc
    20761 gccgggcgtt aaaccgggtg atgtgattat cgccgttgat ccgcgttact tccgtccggc
    20821 agaagttgaa acgctgctcg gcgacccgac caaagcgcac gaaaaactgg gctggaaacc
    20881 ggaaatcacc ctcagagaga tggtgtctga aatggtggct aatgacctcg aagcggcgaa
    20941 aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg agtcataagc
    21001 atgagtaaac aacgcatttt tatcgctggc cattgtggga tggtcggttc tgccatcaag
    21061 cggcagctcg aacagcgcgg tgatgtggag ctggtattac gtacccgcga tgaactgaac
    21121 ctgctggaca gccgcgccgt gcatgatttc tttgccagcg aacgcattga ccaggtctat
    21181 ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca cctatccggc ggatttcatc
    21241 taccagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa cgacgtgaac
    21301 aaactgatgt ttctcggatc gtcctgcatc tacccgaaac tggcaaaaca gccgatggca
    21361 gaaagcgagt tattgcaggg cacgctggag ccgaccaacg agccttacgc cattgccaaa
    21421 atcgccggga tcaaactgtg cgaatcttac aaccgccagt atggacgcga ttaccgctct
    21481 gtcatgccga ccaacctgta cgggccgcat gacaacttcc acccgagtaa ctcccatgtg
    21541 ataccagcat cgctgcgtcg cttccacgag gcgacggcac agaatgcgcc ggacgtggtg
    21601 gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg tcgatgatat ggcggcggcg
    21661 agcattcatg tcatggagct ggcgcatgaa gtctggctgg agaataccca accgatgctg
    21721 tcgcacatta acgtcggcac gggcgttgac tgcactatcc gcgagctggc gcaaaccatc
    21781 gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg
    21841 ccgcgcaaac tgctggatgt gacgcgcctg catcagcttg gctggtatca cgaaatctca
    21901 ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcaaga ccgctttcgg
    21961 ggggaatgat gtttttacgt caggaagact ttgccactgt agtgcgctcc acaccgcttg
    22021 tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc aaaagaacca
    22081 accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc
    22141 tggaagccgc atttgagcgg ctgacgatgg cggaactggg gctgcgtctg ccgataacag
    22201 caggccagtt ttacggtgtc tggcagcatt tttatgacga taacttctcc ggcacggatt
    22261 tcaccactca ctatgtggtg ctcggttttc gcttcagagt agcggaagaa gagctgttac
    22321 tgccggatga gcagcatgac gattaccgct ggctgacgcc ggacgcgctg ctcgccagta
    22381 acgatgttca cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg
    22441 gattatgaaa atactggtct acggcattaa ctactcgccg gagttaaccg gcatcggcaa
    22501 atacaccggc gagatggtgg aatggctggc ggcacaaggt catgaggtgc gggtcattac
    22561 cgcaccgcct tactacccgc aatggcaggt gggtgagaac tattccgcct ggcgctacaa
    22621 acgagaagag ggggccgcca cggtgtggcg ctgcccgctg tacgtgccaa aacagccgag
    22681 caccctgaaa cgcttgttgc atctcggcag ttttgccgtc agcagtttct ttccactgat
    22741 ggcgcaacgt cgctggaagc cggatcgcat tattggcgta gtgccaacgc tgttttgcac
    22801 gccgggaatg cgcctgctgg cgaagctctc tggtgcgcgt accgtgctgc atattcagga
    22861 ttacgaagtg gacgccatgc tggggctggg ccttgccgga aaaggcaaag gcggcaaagt
    22921 ggcacagctg gcaacggcgt tcgaacgtag cggactgcat aacgtcgata acgtttccac
    22981 gatttcgcgt tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg ataacgtcat
    23041 cttcttcccc aactggtcgg aaattgcccg ttttcagcat gttgcagacg ccgatgttga
    23101 tgcccttcgt aaccagcttg gcctgccgga taacaaaaaa atcattcttt actccggcaa
    23161 tattggtgaa aagcaggggc tggaaaacgt tattgaagct gccgatctcc tgcgcgatga
    23221 accgctgatt tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc
    23281 gcagcagcgt ggactgcgca acatgcaatt tttcccgctg caatcgtatg acgctttacc
    23341 cgcactgctg aagatgggcg attgccatct ggtggtgcaa aaacgcggcg cggcagatgc
    23401 cgtattgccg tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg tgattaccgc
    23461 agaggcccac acagaactgg ggcagctttg cgaaaccttt ccgggtattg cggtttgcgt
    23521 agaaccggaa tcggtcgagg cgctggtggc ggggattcgt caggcgctcc tgctgcccaa
    23581 acataacacg gtggcacgtg aatatgccga acgcacgctc gataaagaga acgtgttacg
    23641 tcaatttata aatgatattc ggggataatt atggcgcagt cgaaactcta tccagttgtg
    23701 atggcaggtg gctccggtag ccgcttatgg ccgctttccc gcgtacttta ccccaagcag
    23761 tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg cctgaacggc
    23821 gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat tgtcgcggaa
    23881 cagctgtgtc aactgaacaa actcaccgag aacattattc tcgaaccggc agggcgtaac
    23941 actgcacctg ccattgcact ggcggcgctg gcggcaaaac gtcatagccc ggagagcgac
    24001 ccgttaatgc tggtattggc ggcggatcat gtgattgccg atgaagacgc gttccgtgcc
    24061 gccgtgcgta atgccatgcc ttatgccgaa gcgggcaagc tggtgacctt tggcattgtg
    24121 ccggatctac cagaaaccgg ttatggctat attcgtcgcg gtgaagtgtc tgcgggtgag
    24181 caggatacgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct ggaaaccgcc
    24241 caggcctatg tagcaagcgg cgaatattac tggaacagcg gcatgttcct gttccgcgcc
    24301 ggacgctatc tcgaagaact gaaaaaatat cgcccggata tcctcgatgc ctgcgaaaaa
    24361 gcgatgagcg ccgtcgatcc ggatctcgat tttattcgtg tggatgaaga agcgtttctc
    24421 gcctgcccgg aagagtcggt ggattacgcg gtcatggaac gtacggcaga tgctgttgtg
    24481 gtgccgatgg atgcgggctg gagcgatgtt ggttcctggt cttcattatg ggagatcagc
    24541 gcccacaccg ccgagggcaa cgtttgccac ggcgatgtga ttaatcacaa aactgaaaac
    24601 agctatgtgt atgccgaatc aggcctggtc accaccgtcg gggtgaaaga tctggtagtg
    24661 gtgcagacca aagatgcggt gctgattgcc gaccgtaacg cggtacagga tgtgaaaaaa
    24721 gtggtcgagc agatcaaagc cgatggtcgc catgagcatc gggtgcatcg cgaagtgtat
    24781 cgtccgtggg gcaaatatga ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc
    24841 accgtgaaac cgggcgaggg tttgtcggta cagatgcacc atcaccgcgc ggaacactgg
    24901 gtagtggtcg cgggaacggc aaaagtcacc attgatggtg atatcaaact gcttggtgaa
    24961 aacgagtcca tttatattcc gctgggggcg acgcactgcc tggaaaaccc ggggaaaatt
    25021 ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga tgtggtgcgc
    25081 ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg cgctgtttgt cggatgcggc
    25141 gtgaacgcct tatccgacct acggttcggt tttgtaggcc tgataagacg cggcagcgtc
    25201 gcatcaggca atgaatgcga aaccgcggtg taaataacga caaaaataaa attggccgtt
    25261 tcggtcaggg ccaactattg cctgaaaaag ggtaacgaca tgaaaaaatt aacctgcttt
    25321 aaagcctatg atattcgcgg gaaattaggc gaagaactga atgaagatat cgcctggcgc
    25381 attggtcgcg cctatggcga atttctcaaa ccgaaaacca ttgtgttagg cggtgatgtc
    25441 cgtctcacca gcgaaacctt aaaactggcg ctggcaaaag gtttacagga tgcgggcgtc
    25501 gatgtgctgg atatcggtat gtccggcacc gaagagatct atttcgccac gttccatctc
    25561 ggcgtggatg gcggcattga agttactgcc agccataatc cgatggatta caacggcatg
    25621 aagctggtgc gcgagggggc tcgcccgatc agcggtgata ccggactgcg cgacgtccag
    25681 cgtctggctg aagccaacga ctttcctccc gtcgatgaaa ccaaacgcgg tcgctatcag
    25741 caaatcaacc tgcgtgacgc ttacgttgat cacctgttcg gttatatcaa tgtcaaaaat
    25801 ctcacgccgc tcaagctggt gatcaactcc gggaatggcg cagcgggtcc ggtggtggac
    25861 gccattgaag cccgctttaa agccctcggc gcgcccgtgg aattaatcaa agtacacaac
    25921 acaccggacg gcaatttccc caacggtatt cctaacccgc tgctgccgga atgtcgcgac
    25981 gacacccgca atgcggtcat caaacacggc gcggatatgg gcattgcctt tgatggcgat
    26041 tttgaccgct gttttctgtt tgacgaaaaa gggcagttta tcgagggcta ctacattgtc
    26101 ggcctgctgg cagaagcgtt cctcgaaaaa aatcccggcg cgaagatcat ccatgatccg
    26161 cgtctctcct ggaacactat tgatgtggtg actgctgcag gcggcacgcc ggtaatgtcg
    26221 aaaaccggac acgcctttat taaagaacgg atgcgcaagg aagacgccat ctacggtggc
    26281 gaaatgagcg cccaccatta cttccgtgat ttcgcttact gcgacagcgg catgatcccg
    26341 tggctgctgg tcgccgaact ggtgtgcctg aaaggaaaaa cgctgggcga actggtgcgc
    26401 gaccggatgg cggcgtttcc ggcaagcggt gagatcaaca gcaaactggc gcaacccgtt
    26461 gaggcgatta accgcgtgga acagcatttt agccgtgagg cgctggcggt ggatcgcagc
    26521 gatggcatca gcatgacctt tgccgactgg cgctttaacc tgcgcacctc caataccgaa
    26581 ccggtggtgc gcctgaatgt ggaatcgcgc ggtgatgtgc cgctgatgga agcgcgaacg
    26641 cgaactctgc tgacgttgct gaacgagtaa tgtcggatct tcccttaccc cactgcgggt
    26701 aaggggctaa taacaggaac aacgatgata aatctaaaaa agcgtgagcg agcgaaaacc
    26761 aatgcatcgt taatctctat ggtgcaacgc ttttcagata tcaccatcat gtttgccggg
    26821 ctatggctgg tttgcgaagt cagcggactg tcattcctct acatgcacct gttggtggcg
    26881 ctgattacgc tggtggtgtt ccagatgctg ggcggcatca ccgattttta tcgctcatgg
    26941 cgcggtgttc gggcagcgac agaatttgcc ctgttgctac aaaactggac cttaagcgtg
    27001 attttcagcg ccggactggt ggcgttcaac aatgatttcg acacgcaact gaaaatctgg
    27061 ctggcgtggt atgggctgac cagcatcgga ctggtggttt gccgttcatg tattcgcatt
    27121 ggggcgggct ggctgcgtaa tcatggctac aacaagcgga tggtcgccgt agcgggggat
    27181 ttagccgccg gacaaatgct gatggagagt ttccgtaatc agccgtggtt agggtttgaa
    27241 gtggtgggcg tttaccacga cccgaaaccg ggcggcgttt ctaacgactg ggcgggtaac
    27301 ctgcaacagc tggtcgagga tgcaaaagcg ggcaagattc ataacgtcta tatcgcgatg
    27361 caaatgtgcg acggcgcacg agtgaaaaaa ctggtccatc aactggcgga taccacctgt
    27421 tcggtgctgc tgatccccga cgtctttacc ttcaacattc tccattcacg tctcgaagag
    27481 atgaacggcg ttccggtggt gccgctgtat gacacgccgc tttcgggggt taaccgcctg
    27541 ctaaaacgtg cggaagacat tgtgctggcg acgcttattc tgctgctgat ctccccggtg
    27601 ctgtgctgta ttgcgctggc ggtgaaactc agttcaccag ggccggttat tttccgccag
    27661 actcgctatg gtatggatgg caagccgatc aaagtgtgga agttccgttc catgaaagtg
    27721 atggagaacg acaaagtggt gacccaggcg acgcagaacg atccgcgcgt cactaaagtg
    27781 gggaactttc tgcgccgcac ctcgctggac gaattgccgc agtttatcaa tgtgctgacc
    27841 ggggggatgt cgattgtcgg tccacgtccg cacgcagtag cgcataacga acagtatcgc
    27901 cagctcattg aaggctacat gctgcgccat aaagtgaaac cgggcattac cggctgggcg
    27961 cagattaatg gctggcgcgg cgaaaccgac acgctggaga aaatggaaaa acgcgtcgag
    28021 ttcgaccttg agtacatccg cgaatggagc gtctggttcg atatcaaaat cgttttcctg
    28081 acggtattca agggcttcgt taacaaagcg gcatattgat atgagcttac gtgaaaaaac
    28141 catcagcggc gcgaagtggt cggctattgc cacggtgatc atcatcggcc tcgggctggt
    28201 gcagatgacc gtgctggcgc ggattatcga caaccaccag ttcggcctgc tgactgtgtc
    28261 gctggtgatt atcgcgctgg cagatacgct ttctgacttc ggtatcgcta actcgattat
    28321 tcagcgaaaa gaaatcagtc accttgaact caccactctg tactggctga acgtcgggct
    28381 ggggcttgtg gtatgtgtgg cggtgttttt gttgagtgat gtcatcggcg acgtgctgaa
    28441 taacccggac ctggcaccgt tgattaaaac attgtcgctg gcgtttgtgg tgatccccca
    28501 cgggcaacag ttccgcgcgt tgatgcagaa ggagctggag ttcaacaaaa tcggcatgat
    28561 cgaaaccagc gcggtgctgg cgggcttcac ttttacggta gttagcgccc atttctggcc
    28621 gctggcgatg accgcgatcc tcggttatct ggtcaatagt gcggtgagaa cactgctgtt
    28681 tggctacttt ggacgcaaaa tttattgccc cggtctgcac ttctcgctgg cgtctgtggc
    28741 accgaactta cgttttggtg cctggctgac ggcggacagc atcatccact atctcaacac
    28801 caacctttca acgctggtgc tggcgcgtat tcttggcgcg ggcgtggcag ggggatacaa
    28861 cctggcgtac aacgtggccg tcgtaccgcc gatgaagctg aacccaatca tcacccgcgt
    28921 gttgtttccg gcgtttgcca aaattcagga cgacaccgaa aagctgcgtg ttaacttcta
    28981 caagctgctg tcgatagtgg ggattatcaa ctttccggcg ctgctggggc tgatggtggt
    29041 gtcgaataac ttcgtaccgc tggtattcgg cgaaaagtgg aacagcatta ttccggtgct
    29101 gcaattgctg tgtattgtgg gtctgctgcg ctccgtgggt aacccgattg gttcgctgct
    29161 gatggcgaaa gcgcgggtcg atatcagctt taaattcaac gtattcaaaa catttctgtt
    29221 tattccggcg attgttattg gtgggcagat ggcgggcgca atcggcgtca cgctcggttt
    29281 cctgctggtg cagattatca acaccattct gagttacttc gtgatgatta aaccggttct
    29341 tggttccagt tatcgccagt acatcctgag tttgtggctg ccgttttatc tctcgttgcc
    29401 gacgctggtg gtcagttatg cgctgggcat tgtgctgaaa gggcaactgg cgctggggat
    29461 gctgctggcg gtgcaaatag ccgcgggcgt gctggcgttt gtggtgatga ttgtgctgtc
    29521 acgccatccg ctggtggtgg aagtgaagcg tcagttttgt cgcagcgaaa aaatgaaaat
    29581 gcttttacgg gcggggtgaa ttgctgtttt cggttccgac aacggttgcc ggatgcggcg
    29641 taaaacgcct tatccgtcct acggttctgt gctcgggacc tgtaggcctg ataagacgcg
    29701 ccagcgtcgc atcaggcaaa aaacagcaac gaacacaaca aacacagcct cacctggcat
    29761 caaatttatg aggaaaaaat gaaattactt attctgggca accacacttg cggcaatcgt
    29821 ggcgacagcg ccatcttgcg cggcttactt gatgccatca acatcctcaa tccacatacc
    29881 gaagtggacg tgatgagccg ctatccggtc agttcttcct ggctgctcaa ccgcccggta
    29941 atgggcgatc cgctgttcct gcagatgaca caacacaata gcgcggcggg cgttgtcggg
    30001 cgcgttaaaa aagtcctccg tcgccgctac cagcatcagg tattgctctc acgcgtcacc
    30061 gacactggca agctgcgcaa tatcgccatc gcccagggat tcaccgactt cgtgcgcctg
    30121 ctgtcaggtt acgacgccat tattcaggtc ggcggatcgt tttttgtcga tctctacggc
    30181 gtgccgcagt ttgaacatgc gctttgcaca tttatggcga aaaagccgct gtttatgatt
    30241 ggtcacagcg tcggcccctt ccaggatgag caatttaacc aactggcgaa ctatgtcttt
    30301 ggtcactgcg acgcgctgat cctgcgcgaa tcggtcagcc ttgatttgat gaaacgcagc
    30361 aatatcacca ccgcaaaagt ggaacatggc gtcgataccg cgtggctggt cgatcaccac
    30421 accgaagact tcaccgccag ctatgccgtt caacactggc tggacgttgc cgcacaacag
    30481 aaaacagtgg caattaccct gcgcgaactg gcaccgttcg acaaacgtct cggcaccact
    30541 caacaagcgt atgaaaaagc ctttgccggg gtggtcaatc gcattctcga tgaaggctat
    30601 caggtgattg cgctctccac ctgtacgggt atcgacagct ataacaaaga cgaccgcatg
    30661 gtggcgctca atctgcgcca gcacatcagc gatcctgccc gttaccacgt agtgatggat
    30721 gaactcaacg atctggaaat gggcaaaatt ctcggagcct gtgaactcac cgtcggtacg
    30781 cgcctgcact ccgccattat ctcaatgaac tttgctactc cggcgattgc catcaactat
    30841 gaacataaat ccgccgggat tatgcagcag ctgggactgc cagagatggc gattgatatc
    30901 cgtcatttac tggacggcag cctgcaagcg atggttgcgg ataccttagg ccagcttccg
    30961 gtgctgaacg ctcgacttaa cgaagccgtc agtcgcgaac gtcagacggg aatgcagatg
    31021 gtgcagtctg tgcttgagcg catcggggag gtgaaatgaa ggtcggcttc ttcttattga
    31081 aattcccgct gtcgtcggaa accttcgtcc tcaatcaaat taccgcgttt attgatatgg
    31141 gctttgaagt ggagattgtc gctttgcaaa aaggcgatac agagaacacc cacgcggcat
    31201 ggacgaaata caaccttgtc gccagaaccc gctggttaca ggacgaacct gcgggcaaag
    31261 tagcgaaact gcgccaccgc gccagccaga ccttacgtgg cattcatcgt aaaaatacct
    31321 ggcatgcgct caacctcaaa cgctatggtg ccgagtcgcg gaacctgatt ttgtctgcca
    31381 tttgtggtca ggtcgcaaca ccgtttcgcg ccgatgtgtt catcgctcat tttggcccgg
    31441 cgggggtaac cgcagcaaaa ctacgcgaac tgggtatcat tcgcggcaaa attgccacca
    31501 tcttccacgg tattgatatc tccagtcggg aagtgctcaa ccactacact cccgaatatc
    31561 aacaactgtt tcgccgtggc gacctgatgt taccgataag cgatctgtgg gccggaaggc
    31621 tgcaaaaaat gggctgcccc agagaaaaaa tcgccgtatc gcgtatgggc gtggacatga
    31681 cgcgctttag cccgcgtccg gtgaaagcgc ccgcaacgcc gctggaaatc atatctgtcg
    31741 cacgcttaac cgagaaaaaa ggcctgcatg tggcgattga agcctgccgt cagttgaaag
    31801 agcagggcgt ggcatttcgc tatcgcatcc tcggcattgg cctgtgggaa cgacgcctgc
    31861 gcaccctcat cgaacaatat caactggaag atgtggtaga gatgccgggc tttaaaccga
    31921 gccatgaagt gaaggagatg ctcgacgacg cggatgtctt cctgttgcca tcggtaacgg
    31981 gcgcggatgg cgatatggaa ggcattccgg tggcgctaat ggaagcgatg gcggtcggta
    32041 ttccggtggt ttctactctg catagcggaa ttcctgaact ggtggaggcc gataaatccg
    32101 gctggctggt gcctgagaac gatgctcgcg cactggcaca acgtctggcg gcgtttagcc
    32161 aactggatac cgacgaactg gctccggtcg tcaaacgtgc gcgcgaaaaa gtcgaacacg
    32221 attttaacca gcaggtgatt aatcgagaac tcgccagctt gctgcaggct ttatagagga
    32281 gaacgtatgc catttaaaac actctcccga cgcaccttcc tgacggcaag ctcggcgctt
    32341 gccttcctcc ataccccttt cgctcgcgca cttcccgccc gacaaagcgt taacattaac
    32401 gactacaacc cacatgactg gatcgcctca tttaaacaag ccttcagcga agggcaaacg
    32461 gtcgtcgtgc ctgccggatt cgtttgtgac aatatcaaca ccggcatctt catccctccc
    32521 ggcaaaacgt tacacatcct tggaagcctg cgcggcaacg gcagagggcg atttgtctta
    32581 caggacggca gccaggtgac agggggagag ggcggcggta tgcataacat taccctggat
    32641 gtgcgtggtt ctgactgcac catcaaaggg ctggcgatga gcggctttgg cccggtaatg
    32701 cagatttata ttggcggcaa aaacaaacgg gtcatgcgca acctgaccat cgataacctc
    32761 actgtcagcc acgctaatta cgccatctta cgccagggat ttcataacca gattatcggt
    32821 gccaacatta ccaactgtaa gttcagcgac ttacagggcg acgccattga atggaacgtg
    32881 gcaattaacg acagtgatat tttgatatct gaccatgtca tcgagcgtat caactgtacc
    32941 aacggcaaaa tcaactgggg catcggcata ggccttgcgg gaagcactta tgataacaac
    33001 tacccggaag accaggcagt gaaaaacttt gtcgtggcga atatcacggg atcggattgt
    33061 cggcagttga tccatgttga aaatggtaaa cattttgtta ttcgtaatat caatgcccgc
    33121 aatatcacgc cggatttcag taagaaagcg ggcattgata acgcgacagt cgctatttac
    33181 ggttgtgaca atttcgtgat tgataatatt gaaatgatta atagcgccgg gatgttaatc
    33241 ggctatgggg taattaaagg caaatatctc tcgataccgc aaaatttccg agtgaataat
    33301 attcaactgg ataataccca tcttgcttat aaattgcgcg gcatccaaat ctccgccggg
    33361 aatgccgtct cctttgtgtc gctaactaac attgagatga agcgtgcctc gctggagtta
    33421 cacaacaagc cgcaacatct ttttatgcga aatatcaatg tgatgcagga atcctcagtt
    33481 ggaccagcat tgagcatgaa cttcgacatg cgcaaagacg ttcgcggcgt ctttatggcg
    33541 aaaaaagaaa cactgctgtc tcttgcaaat gttcatgcgg tgaatgaaag agggcaaagc
    33601 tccgtcgata tcgacaggat taatcaccat attgttaatg tggaaaagat taactttaga
    33661 ttgccggaac ggagggagta gatttgcgac cattcctgga aaaatggagc catacttagg
    33721 aacaatgcta ctgcaatcca caacgaagcg gcgtaacatc acaagtaatt cagtaatcaa
    33781 ttcagggtaa ttgatgctgg cgaaaaaaat cgaacaagct ataattcagc aaccatttta
    33841 caggtggatg aaataatgac gaatttaaaa gcagttattc ctgtagcggg tctcgggatg
    33901 catatgttgc ctgccactaa ggcgataccc aaagagatgc taccaatcgt cgacaagcca
    33961 atgattcagt acattgttga cgagattgtg gctgcaggga tcaaagaaat cctcctggta
    34021 actcacgcgt ccaagaacgc ggtcgaaaac cacttcgaca cctcttatga gttagaatca
    34081 ctccttgagc agcgcgtgaa gcgtcaactg ctggcggaag tacagtccat ctgtccgccg
    34141 ggcgtgacca ttatgaacgt gcgtcagggc gaacctttag gtttaggcca ctccattttg
    34201 tgtgcgcgac ctgccattgg tgacaaccca tttgtcgtgg tactgccaga cgttgtgatc
    34261 gacgatgcca gcgccgaccc gctacgttac aaccttgctg ccatgattgc acgtttcaac
    34321 gaaacgggcc gcagccaggt gctggcaaaa cgtatgccgg gtgacctctc tgaatactcc
    34381 gtcatccaga ctaaagagcc gctggaccgt gagggtaaag tcagccgcat tgttgaattt
    34441 atcgaaaaac cggatcagcc gcagacgctg gactcagaca tcatggccgt aggtcgctat
    34501 gtgctttctg ccgatatttg gccggaactg gaacgtactc agcctggtgc atggggacgt
    34561 attcagctga ctgatgctat tgccgagctg gcgaaaaaac aatccgttga tgcaatgctg
    34621 atgaccggcg acagttacga ctgcggcaaa aaaatgggct atatgcaggc gtttgtgaag
    34681 tatggcctac gcaacctgaa agaaggggcg aagttccgta aaggtattga gaagctgtta
    34741 agcgaataat gaaaatctga ccggatgtaa cggttgataa gaaaattata acggcagtga
    34801 agattcgagg cataagtttt gctcattgaa ttttcctgcc gttgccgttt ttataaagcc
    34861 tataaaaaca agaagttaca tagctcatat tattcaaatt tttccaggat tttccttgtt
    34921 tctacggctg tttggtaaga caattaacgt ttgaattttt cggatttggc gcgagtggct
    34981 aacgctcgcc acatcgttga gatgcatgca gtgcactggt agctgttaag ccaggggcgg
    35041 tagcgtgtat taatacctct attaatcaaa tctggagcag tctatttcac agcatgctct
    35101 atagctatat ggaataaaaa agtgaagata cttgttactg gtggcgcagg gtttattggt
    35161 tctgctgttg ttcgtcacat aataaataat acgcaagata gtgttgttaa tgtcgataaa
    35221 ttaacatacg ccgggaacct ggaatcactc gctgatgttt ctgattctga acgttatgct
    35281 tttgagcatg caggtatctg cgatgctgta gccatgtcgc gtattttcgc acagcaccag
    35341 ccagacgcgg tgatgcacct ggcggcagag agccacgttg accgttcaat aacgggccct
    35401 gcggcattta ttgaaaccaa tattgtgggg acttatgttc ttttagaagc ggcgcgcaat
    35461 tactggtctg ctctgaatga tgaaaagaaa aaaagtttcc gctttcatca tatttctact
    35521 gatgaagttt atggtgactt accccatccg gatgaagcaa ataataacga agcgttaccg
    35581 ctatttacgg aaacgacagc ttacgcgcca agtagcccgt attctgcgtc taaagcttcc
    35641 agcgatcatt tggtacgtgc ctggaagcgg acttatggtt taccaaccat tgtgactaat
    35701 tgctcgaaca attatggtcc ttatcatttc ccggaaaaac ttattccatt ggttattctg
    35761 aatgctctgg aaggtaaggc attacctatt tatggtaaag gggatcaaat tcgcgactgg
    35821 ttgtatgtag aggatcatgc tcgtgcgtta tataccgtcg taaccgaagg taaagcgggt
    35881 gaaacttata acattggtgg acataacgaa aagaaaaaca tcgacgtagt gctcactatt
    35941 tgtgatttgt tggatgagat tgtaccgaaa gagaaatctt atcgtgagca aattacttat
    36001 gttgccgatc gcccggggca tgaccgccgt tacgccattg atgccgataa aattagccgc
    36061 gaattgggct ggaaaccgca agaaacgttt gagagcggga ttcgtaaaac ggtggaatgg
    36121 tatctggcta atacaaattg ggttgagaat gtgaaaagcg gtgcttatca gtcgtggatt
    36181 gaacaaaact atgagggccg tcagtaatga atatcctgct tttcggcaaa acagggcagg
    36241 tgggttggga actgcagcgt gctctggctc ctctgggtaa cttgattgcg ctcgatgttc
    36301 actccactga ttattgtgga gatttcagca accccgaagg tgtggctgaa accgtcaaaa
    36361 aaattcgccc cgatgttatt gttaatgctg ctgctcacac tgcagtagac aaagcggaat
    36421 cagaaccgaa tttcgcgcaa ttacttaacg cgacatgcgt cgaagcgatt gcaaaagcag
    36481 ctaatgaagt cggagcctgg gttatacact actccactga ttatgttttc ccaggcaacg
    36541 gtgacacgcc atggctggaa acggatgcaa cagcaccgct aaatgtctac ggtgaaacca
    36601 agctagctgg ggaaaaagcg ttacaagaac attgcgcaaa gcatcttatt ttccgtacca
    36661 gctgggtata cgctggtaaa ggaaataact ttgccaaaac gatgttgcgc ctggcaaaag
    36721 aacgcgaaga gttggctgta ataaacgatc aattcggcgc accaacgggg gctgaattac
    36781 tggctgattg caccgctcat gccattcgcg tggccgcaaa taaaccagaa gttgctggtt
    36841 tgtaccatct ggttgctggt ggtacaacaa cctggcacga ttatgccgct ctggtatttg
    36901 aagaagcgcg cagagcgggg atcaaccttg ctcttaacaa acttaatgcc gtgccgacaa
    36961 cggcctatcc cacaccagca cgtcgtcccc ataattctcg cctcaatacc gaaaaatttc
    37021 agcagaactt tgcgcttgtc ttgcctgact ggcaggtggg cgtgaaacgt atgctcaacg
    37081 aattatttac gaccacggca atttaacaaa ttattgcatc tcgctcatga tgccagagcg
    37141 agatgaatta aaaggaatgg tgaaatgaaa acgcgtaaag gtattattct ggctggtggt
    37201 tccggcactc gtctttatcc tgtgactatg gctgtgagca aacaactgct accgatttat
    37261 gataaaccga tgatttatta tccgctttca acgcttatgt tggcgggtat tcgagatatt
    37321 ctgattatca gtacgccgca ggatacacca cgttttcaac aattgcttgg tgatggaagc
    37381 cagtgggggc ttaatctgca atataaagta caaccgagtc cggatggtct ggcgcaagca
    37441 tttatcattg gtgaagaatt tataggtggt gatgattgtg cgctggtact tggtgataat
    37501 atcttctatg gtcacgacct gccgaaatta atggataccg ctgttaacag agagagtggc
    37561 gcaacggttt ttgcctatca cgtaaatgat ccagagcgct acggtgtggt ggaatttgat
    37621 gataacggta cggcaattag tctggaagaa aaaccgctgg aaccaaaaag taactatgcg
    37681 gttactgggc tttatttcta tgacaatgac gttgtagaaa tggcgaaaaa ccttaaacca
    37741 tctgcacgtg gcgaactgga aattaccgat attaaccgta tttatatgga ccaaggacgt
    37801 ttgtctgtcg ctatgatggg gcgtggttat gcatggctgg acacggggac acatcaaagt
    37861 cttattgaag caagcaactt cattgcaaca attgaagagc gtcaggggct gaaagtttcc
    37921 tgcccggaag aaattgctta ccgtaaaggc tttattgatg ctgagcaagt gaaggtatta
    37981 gccgagccgc tgaagaaaaa tgcttatggt cagtatctgc tgaaaatgat taaaggttat
    38041 taataaaatg aacgtaatta aaactgaaat tcctgatgtg ctaatttttg aaccaaaagt
    38101 atttggtgat gagcggggct tttttatgga aagcttcaat cagaaagttt tcgaagaggc
    38161 tgtaggacga aaggttgaat ttgttcagga taaccattct aaatcaacta agggtgtgtt
    38221 acgcggactg cactatcagc tggaacctta tgctcaagga aaactggtac gctgtgttgt
    38281 cggtgaagtt tttgacgtag ctgttgatat tcggaaatca tcaccaacat ttggcaagtg
    38341 ggttggtgta aatttgtcgg cggagaataa gcgtcaatta tggataccgg aaggattcgc
    38401 tcatgggttt tgcgttctta gtgatgaagc agaatttgtc tacaaaacaa ataatttcta
    38461 ttcaaaaatg caagagcgtg ggattttatg gagcgacaaa agtataaata tagaatggca
    38521 ggttcaaaat ccattgcttt ctgataaaga tattaatggt caaaaatttg tagatgctga
    38581 ttattttata tgataaagaa atgtaataat gagcataata aaaaatagtg tctggaacct
    38641 ttttggctat gcaataccaa ctttaattgc tatcccctcg ctaggatttc tcgctcgagg
    38701 tttggggcct gaaggtttcg gtgtttatac aattgcaatt gcacttgttg ggtatgctgg
    38761 aatttttgat gtaggcctga ctcgctctgt tattagagaa attgcaattc atcgtgataa
    38821 tcatcatgaa agaaccaagg taatttcaac aagtacatct tttttggtgc tattttcatg
    38881 ctttggtgct tttttattat tgattttctc tgatggaatt gttaattatc taaaaatttc
    38941 tggtgttgaa catagtgata tacaactagc atttaaactg ttggctattt gcattccatt
    39001 atttattcta aatcaattat ggtcagccat tcttgagggg gatgaaaaat ttggcattgt
    39061 aaatattcaa aaatctatat caagctcttg catcgcggga attccggcca tatttgtttt
    39121 ttatagtgct acattgtcgg cagcggttgc tggtttaata tttgcaagag ttatttcgat
    39181 tttagtctct gcctattatg tcaggaatga tattaaaatt tcgggggttc atttttgtta
    39241 taaaaccttt aaacgactct ttttctttgg cggttggatg acagtaagta atatcataag
    39301 tccggtcatg gtgtattttg atcgatttat agtgtcaaat attatggggg cagataaagt
    39361 tgcattttat tctgcaccag cggaggttat tctaaaatta ggaataatac ctgcagcaat
    39421 cgggagggca gtgtttccaa ggttaagtaa catcaaagac tttaaagaat ttaaacgtaa
    39481 tgtaaataaa tcattgcttt taatgtttct aatctgtttg ccggtgataa tcataggctt
    39541 gttatattca gggcttgtat tgaaaatatg gtttggtgag aattaccaaa ttaattcctt
    39601 taatatatta aatgtgttat tgatcggttt tttcttcaat gcgctggcaa tgataccatt
    39661 ctctgcaatc caggcattag gaaaatctaa aattactgct ttgattcatt gtgctgaatt
    39721 ggttccttat ttagcccttt tgtattttat ggtcgaaaaa tatgggttac tgggtgccgc
    39781 aatatcctgg agcatacgtg taattttaga tgctctgtta ttacaatggc tttatactag
    39841 aatgtgttct gtatatgaaa actaaaatta accaattact gagtcacttg gataatgaat
    39901 agtaatattt acgctgtcat tgtgacatat aatcccgaac ttaaaaatct gaatgcactg
    39961 atcactgaac taaaggagca aaattgttac gttgtagtag tcgataatag tactaatttc
    40021 accttgaaag ataaattagc agacattgaa aaagttcatt taatttgtct tggtaggaat
    40081 gaaggtatag caaaagcaca aaatattggg attcgatatt ctcttgaaaa aggggcagag
    40141 aagataattt tttttgatca agatagccgt attcgcaatg agtttatcaa aaaactttcc
    40201 tgctatatgg ataatgaaaa tgcaaaaata gctggcccag tttttattga tagagataag
    40261 tcacattatt atcctatttg taatatcaaa aaaaatggtc ttcgagagaa aattcatgtc
    40321 actgaaggac agacaccgtt taaaagttca gtaacaatct catccggaac catggtttca
    40381 aaagaagttt ttgagattgt tggaatgatg gatgaggaac tttttattga ttatgtcgat
    40441 acagaatggt gtcttagatg cttaaactat ggcatattag ttcatatcat tcctgatata
    40501 gaaatggttc atgctattgg ggataagtca gtaaaaatct gtgggattaa cataccaatt
    40561 cactcgccag tacgtcgtta ttatcgagta agaaatgcat ttcttttgct tagaaaaaat
    40621 catgtgcctc ttttactctc tattagggaa gttgtttttt ctttaattca tacgacttta
    40681 attatcgcaa ctcaaaaaaa taaaattgaa tatatgaaaa aacatatttt ggctacgctg
    40741 gatggaataa gaggaattac tggggacggt cgttataatg catagttctg atcaaaaaag
    40801 agtagctgta cttatggcta cgtataatgg cgaatgctgg attgaggaac aattaaaaag
    40861 tattattgaa caaaaagatg tagacatctc aatttttata tcagatgatt tatcaactga
    40921 taatacatta aatatttgtg aagaatttca attatcatat ccctcaatta taaatatact
    40981 tccaagcgtc aataagtttg ggggggcagg taaaaatttt tatagattaa ttaaagatgt
    41041 cgatcttgaa aactatgatt atatctgttt tagtgatcag gacgatattt ggtataaaga
    41101 taaaataaaa aatgcaattg actgcttggt atttaataat gcgaattgtt attccagcaa
    41161 tgtaattgca tattatccat cagggcaaaa aaatttagtt gataaggctc agtcgcaaac
    41221 tcaattcgat tatttttttg aagcagcagg tccggggtgt acatatgtta taaaaaaaga
    41281 aacgcttatt gaatttaaga agttcatcat aaataataaa aatgctgcac aagatatctg
    41341 tctgcatgat tggtttttat attcatttgc gcggacgaga aattattcgt ggtatattga
    41401 tagaaaacca acaatgctgt acagacagca tgaaaacaac caagtgggag caaacattag
    41461 ttttaaagca aaatacaaac ggcttggttt ggtgagaaac aagtggtatc gaaaagaagt
    41521 aactaaaata gctaatgcct tagctgatga tagctttgtg aataatcaat tgggtaaagg
    41581 ttatataggc aatttgatct tggctttaag tttttggaag ttaaggcgga aaaaggctga
    41641 taaaatttat attttattaa tgttgatttt aaacatattt taaaaaacat caccttacga
    41701 atatgaataa tataaataaa atttttataa catttttatg tattgaactg attattggtg
    41761 gtggtggaag attactggag ccattgggaa tattcccttt gcgatattta ttatttgtat
    41821 ttagttttat acttttaatt tttaatttag ttacattcaa tttttcaatc acccaaaaat
    41881 gtgtcagtct ttttatatgg ttgcttttat ttccttttta tggcttcttt gtcggcttat
    41941 tagctggtaa taaaataaat gatatactgt ttgatgtgca accatacctc tttatgctgt
    42001 cacttatata tctatttaca ctaagatata ctttaaaagt atttttatgt gagattttta
    42061 ttaaaatagt taatgcattt gcattatatg gatcactgtt atatatttca tacataattt
    42121 tgttgaattt cggtttgtta aattttaatt taatttatga acacttatca ttgactagcg
    42181 agttcttttt tcgtcccgat ggggcttttt tttccaaatc cttctacttt tttggtgtcg
    42241 gtgcgattat cagttttgtc gacaaaaaat atttaaaatg tctcataata gtgcttgcga
    42301 tattattgac agaatcaaga ggtgtattac tttttacaac attatcactg ttattagcca
    42361 gttttaaatt acataagcta tatttaaata ctattataat aatattgggc agcgttctat
    42421 ttataattat gctttacatg gtcggatcac gcagtgaaga ttctgactct gttagattta
    42481 atgatttata tttttattat aaaaatgttg atttagcgac gttcttgttt ggaagaggat
    42541 ttggttcatt tatattagat cgattaagga ttgaaatagt acctcttgag atacttcaga
    42601 aaacaggcgt tattggtgta tttatatcat tagttcctat gttgcttatc tttttgaaag
    42661 gctatttttt aaacagtgca aaaacatcat taatgatgtc gttaatactt tttttcagta
    42721 ttaccgtttc tataactaat ccatttcttt ttacacccat gggaattttt attataggcg
    42781 ttgtagtttt atgggtattt tctatagaaa atatccaaat tagtaataac ctcacttctg
    42841 gagcaaaata aaggatgtta aaaataggga agttattgac cagttctttt tttagttatt
    42901 ttttaattgg aattgtcaat actgctttac attggggggt attttatgcg tgttataata
    42961 atttagcttt tggtcaaggc cgaagtaaca ttgttggttt tatatgtgca gcaacctttt
    43021 ctttttttgc aaatgcaaga tgctcattta aagtatctgc aaccaaggct agatatttta
    43081 tatttatatt ttttatgggg gcgatgagtt atttattcgg cgttttattt gatctcttag
    43141 ctttatctcc aatatttaca ttgttcacct tttctctttt tagtttggtt ttaggatatt
    43201 gcgcatcaaa atatttcatt tttcgttagt aaacgatttg ttaaaaacac aattacttgc
    43261 acattagcta aatagtttat atgaggggct tatgtatcac tcttttaata tcgctgtaat
    43321 tattccttgt tataatgagc agaaggcaat agcaaaagtc ataaatgatt tcaaaacgaa
    43381 tattccaact gcttctatat atgtatttga taataattcg actgacagta cagcacaagt
    43441 ggcagaggat gctggagcaa cagttcactc tgttcctctg aaaggcaagg ggaatgtcgt
    43501 aagacgtatg ttctcagatg tagatgcgga tatttatcta atggttgatg gagatgacac
    43561 ttatgatgca agttctgctc cagaaatgat taaccatctc ataaaaaatc aattggatat
    43621 ggttgtagga tgtcgtcaag agaatggaga tcaaaataca tacagaaaag ggcatcgctg
    43681 aggaaataaa ctgttgactg gtactgttca gactatcttt aaagggaatt ttactgatat
    43741 gttgtcgggg tatcgtgtat tttccagacg ctatgttaaa tcttttccat gtctatcaag
    43801 gggatttgaa atcgaaactg agcttacaat tcatgcttta gaattacgta tgaagtatgg
    43861 tgaggttaat acaaaatatg gcgagcgatc tgaagggagt gtgagcaaac tatcaacttg
    43921 gagtgatggg ttcaagattc tgaaaacaat aattaaactt tactcgcttg aacggccact
    43981 atactttttt agtattatcg gcgttttact tgctgctttg tctataattt taggattgcc
    44041 catcattgtt gattatattg atacaggttt ggtgagaaga tttccgactg cctttttaac
    44101 tgcctccatc atgttatcgt caataatggc atttgtatgt ggcattattt tgcattcaaa
    44161 tacaacaact agacgtgaaa tgaaggcatt attttatctt tctgaaaaaa aactataaac
    44221 tgataatgta aatagagtat tttactatga ttgaattgcc gttgtatttg tatgttgtgt
    44281 attgttttgc ttttggaatg acattttttg tcattggtaa tattgtattg tcatcttttt
    44341 ttaaggaggg ggtggcagga cttaacaaat tttctttatt tttttattgc ctatgcatag
    44401 ggatgtcttt aagtattttg ataatttact ttctgggcat ggtcgggcaa tttaataaga
    44461 ttgctatctt tttaattttt acggtgtgtt gggttttaag cattattaaa cgtcagcaat
    44521 ttagatggct ggcaataaat aatattgagt tcagtacgct gtttgttatt ttatttcttg
    44581 ttttaatttt tgcggttaca ttgttatcgt ccctgcgagc tcccggtgat tgggatgata
    44641 ctatgtatca cttacctttg gctcgttcat tagttgagca ccatgcaatt gttgttgaac
    44701 aatatttgcg ttttcctctg ttcccgcaaa atgcagatct tttaatggct ttaggtttac
    44761 agttaggaga tgtaaggtta gctcagtttt tagcaaatat ctgttttttt gtaatagcct
    44821 gtggacttgt tggctgttca tgggaaatta cgaaaactta ttatcttggc attattgcta
    44881 caatcctact atttactatc aatccactta aagatcatct tggttatgca tatattgatt
    44941 taacgctatc tttgttttgc tgcagccagt attcttatat atattcgctc agaaaacaat
    45001 aaaacacaaa atgaattaat ttttttatgt ggttttttta ttggaattgc ttgtggcgta
    45061 aaactatttg gtgccatata tggagccatc atatttgtta tattgatagc gaaaaagaga
    45121 aacatacaga catttcttat ttttagcttg gcaacaattc ttggtggcat ttattggtat
    45181 gtccgtagtt tttggatctc tggtgatcct tttagtcctg ctggaggaaa tatatttggt
    45241 cactacctat ggaatgaaat tgatctacaa gttcaaacag cagaacaagc tcgacatggt
    45301 ataagtcctg ccacattaga tttgcaacaa gcaattaata aagttggcag tgatgttgtt
    45361 ttttatgcta tactgtctgt ctttacactt aggaataata aaatactttg ggtatttttc
    45421 tcgattgttt taacatatgt cttattttgg ttttctgtta cgcaaggtga caggtattta
    45481 tcacccattt acccattgtc agtattgctg gttgcgatct caattgcaag tttaataaaa
    45541 gatatcaaca ttacaaatta caagttggga cgatatttta tccttgtgtg tattattttt
    45601 tttcggggcg atcgttgtat tttgaattca aatattttta ttctgaatac ttgaatttta
    45661 attcacgctt agaaagtcgc tatagctatg aactgatgaa gaaagcaagt gaatatagtg
    45721 agttatatgg agataatctt atccaattag gcttggagga tggtatttac ttctataaag
    45781 ggatggcaat tggtgacgtc tttggattag caagatatag tgattggact attagtaatc
    45841 ccgagtgtga ggtaatccca caggatgatt taatagagaa gatgaaatct tttaatagtt
    45901 cgtttatttt tatatctaaa cgaagctatg ctaattttaa tccagaaaag tatccaaaat
    45961 tcaaagttct tatggataca cccaatggca tacttatcgc tataaagtaa gaagcatgct
    46021 gtaaccatat gtattccttg attagaagat gaaaatcacg acggttaata atataacggt
    46081 taatagacgg ttaataatat agaagacagg agtaaacaat gtcaaagcaa cagatcggcg
    46141 tagtcggtat ggcagtgatg gggcgcaacc ttgcgctcaa catcgaaagt cgtggttata
    46201 ccgtctctat tttcaaccgc tcccgcgaaa agacggaaga agtgattgct gaaaatccag
    46261 gcaagaaact ggctccttac tatacggtga aagagtttgt tgaatctctg gaaacgcctc
    46321 gtcgcatcct gttaatggtg aaagcaggtg caggcacgga tgctgcgatt gattccctga
    46381 aaccatacct cgataaaggt gacatcatca ttgatggtgg taataccttc ttccaggaca
    46441 ccattcgtcg taatcgtgag ctttcagcag agggctttaa cttcatcggt accggtgttt
    46501 ccggcggtga agagggcgcg ctgaaaggtc cttctattat gcctggcggg cagaaagagg
    46561 cttatgagct ggttgcgcct atcctgacta aaatcgctgc cgtggcagaa gacggcgagc
    46621 catgcgttac ttatattggt gccgacggcg caggccatta tgtgaagatg gttcacaatg
    46681 gtattgaata cggagatatg cagctcattg ctgaagcgta ttctctgctc aaaggtggcc
    46741 tgaatctatc taatgaagaa ctggcccaga ctttcaccga gtggaataac ggtgaactta
    46801 gcagctacct gatcgacatc actaaagata tcttcaccaa aaaagatgaa gacggtaact
    46861 acctggttga tgtgattctg gatgaagcag caaacaaagg caccggtaaa tggaccagcc
    46921 agagcgcgct ggatctcggc gaaccgctgt cgctgattac cgagtctgtg tttgcacgtt
    46981 atatctcttc tctgaaagat cagcgcgttg ccgcatctaa agttctctct ggtccgcaag
    47041 ctcagtcagc aggcgacaag gctgagttca tcgaaaaagt tcgcagtgcg ctgtatctgg
    47101 gcaaaatcgt ttcttacgct cagggcttct ctcagctgcg tgctgcgtct gaagaataca
    47161 actgggatct gaactacggc gaaatcgcga agattttccg tgctggctgc atcatccgtg
    47221 cgcagttcct gcagaaaatc accgatgctt atgccgaaaa tccgcagatc gctaacctgc
    47281 tgctggcccc gtacttcaag caaattgccg atgactacca gcaggcgctg cgcgatgtcg
    47341 tcgcttatgc agtacagaac ggtatcccgg ttccgacctt cgccgctgcg gttgcctatt
    47401 acgatagcta ccgtgccgcg gttctgcctg cgaacctgat ccaggcacag cgtgactatt
    47461 tcggggcgca tacttataag cgcattgata aagaaggtgt gttccatact gaatggctgg
    47521 attaatctga tttaaccaac aataaaattg aggcccggcg tatattgcac cgggcttttt
    47581 tttgccaaat atcttataga ctaaatttca ctgcttaata ttaacttaat aaacatcagc
    47641 tattcttata aagaaaatct gaattgtttt tcgttgcgtt gaccatctga caacgtagcg
    47701 ttaaaacttt tagcatctta tcacgatgct aaaaacatca tgattcacag ttaagttaat
    47761 tctgagagca tgaaatgaaa atcaccattt ccggtactgg ctatgtaggc ttgtcaaacg
    47821 ggcttctaat cgcacaaaat catgaggttg tggcattaga tattttaccg tcacgcgttg
    47881 ctatgctgaa tgatcggata tctcctattg ttgataagga aattcagcag tttttgcaat
    47941 cagataaaat acactttaat gccacattag ataaaaatga agcctaccgg gatgctgatt
    48001 atgtcatcat cgccactcca accgactatg atcctaaaac taattatttc aatacatcca
    48061 gtgtagaatc agtaattaaa gacgtagttg agataaatcc ttatgcggtt atggtcatca
    48121 aatcaacggt tcccgttggt tttaccgcag cgatgcataa gaaatatcgt actgaaaata
    48181 ttattttctc tccggaattc ctccgcgaag gtaaagccct ttacgataat ctccatccgt
    48241 cacgtattgt catcggtgag cgttcagaac gcgcagaacg ttttgctgcg ttgttacagg
    48301 aaggcgcgat taagcaaaat atcccgaccc tgtttaccga ctccactgaa gcagaagcga
    48361 ttaaactttt cgctaatacc tatctggcga tgcgcgtagc atactttaat gaactggata
    48421 gttatgcaga aagtttaggt ctgaatactc gtcagattat cgaaggcgtt tgtctcgatc
    48481 cgcgtattgg caaccattac aacaatccgt cgtttggtta tggtggttat tgtctgccga
    48541 aagataccaa gcagttactg gcgaactacc agtctgtgcc gaataacctg atctcggcaa
    48601 ttgtcgatgc aaaccgcacg cgtaaagatt ttattgccga tgccattttg tcacgcaaac
    48661 cgcaagtggt gggtatttat cgtctgatta tgaagagcgg gtcagataac ttccgtgcgt
    48721 cttctattca ggggattatg aagcgtatca aggcgaaagg cgttgaagtg atcatctacg
    48781 agccggtgat gaaagaagac tcattcttca actctcgcct ggaacgtgat ctcgccacct
    48841 tcaaacaaca agccgacgtc attatctcca accgtatggc agaagagctt aaggatgtgg
    48901 cagataaggt ctacacccgc gatctctttg gcagcgacta acatcttgtt atcagggcta
    48961 tttacgccct gattgtcttt tgttacctcc gcaataattc attattttta tcacttatcc
    49021 tatagcattc acggggatta tcgctaaact atgcggactt ggaaatttcc gtcagttagg
    49081 gtaatgatga gagtagaaaa taataatgtt tctgggcaaa accatgaccc ggaacagatt
    49141 gatttgattg atttactagt gcagttgtgg cgtggcaaga tgacaattat catttccgtc
    49201 attgtggcta ttgccctggc tattggttat ttggcagtag cgaaggagaa atggacgtca
    49261 acagcaatta tcactcagcc cgacgtgggg caaattgctg gctataacaa tgccatgaat
    49321 gttatctatg gtcaggctgc accgaaagta tcggatttgc aggagacgtt aattggtcgc
    49381 ttcagttctg ccttctctgc attagcagaa acgctggata atcaggaaga gccagaaaaa
    49441 cttaccatcg aaccttctgt taagaaccag caattaccat tgactgtttc ttatgttggg
    49501 caaactgcag agggcgcaca aatgaagttg gcccaataca ttcagcaagt tgatgataaa
    49561 gtgaatcaag agctagaaaa ggatctcaag gacaacattg ctctgggacg gaaaaacttg
    49621 caggactctt taagaaccca ggaagtggtc gcgcaggagc agaaagatct gcgtatccgt
    49681 cagattcagg aagcgttgca gtatgcgaat caggcgcagg tgacaaagcc acaggttcag
    49741 cagactgaag atgtgacgca agatacgttg ttccttctag ggagcgaagc gctggagtcg
    49801 atgattaagc atgaagcgac tcgtccgttg gtgttctcac caaactacta tcagacacgt
    49861 caaaacctgt tggatattga aaaattaaag tttgatgatc ttgatattca tgcttaccgc
    49921 tatgtgatga aaccgacgtt acctattcgt cgcgatagtc cgaaaaaggc aatcaccttg
    49981 attctggcag tgcttctggg cggcatggtt ggcgcgggga ttgtgttggg gcgtaacgct
    50041 ctgcgtaatt acaacgcgaa gtaatattat tgtgcattta agagaaacgg gcagggtggt
    50101 gacaccatgc ccgttttttt tgccggatgc gatgctggcg catcttatcc ggcctacgtg
    50161 tgttgagata atgtgtaggc acgataagtt tgcgcatcgg gcaatggctc cgggtgtgac
    50221 aacaacatca cacctgctca gcccgtaact cactgatgcc gtttacgcag gttctcaatt
    50281 actgtcgtta aatccagatc ctgatcctgc aacagcacca gcaggtgata catcaagtca
    50341 gatgcctcgt tggtcagctc aaagcgatca tgtaccgttg ctgccagtgc ggtttccacg
    50401 ccttcttcgc ccactttctg cgcaatgcgt ttagtgccgc tggcatacag tttggcggtg
    50461 taggaggttt ccgggtcggc atatttacgt tcggcgagca gttgttccag ttgatacagg
    50521 aacagccact ggtgagcggt gttgccgaag cagctactgg tgcctttgtg gcaggttggc
    50581 ccgatggaat tcgccagcac cagtaatgtg tcgttgtcgc agtccggggc aatacttacc
    50641 acattgagaa aattgcccga ggtttcgcct ttggtccaca gtcgctgttt agtgcgcgag
    50701 aagaaggtga ctttaccgct ttcgatggtt ttgtctaagg cttccgggtt catgtagccc
    50761 agcattaaca cttcgccaga taccgcgtgt tgcacaatca ccggcatcag accgtcggtt
    50821 ttttcccagt ccagttcgcg acgttgttgt tctgttaaca tatcctgatc tccacgccct
    50881 gtgttgccag gtacgctttt aattcgccaa tattgattat ttgtttgtgg aatacggaag
    50941 ctgccagcgc accgtcaacg tcggcatcgc ggaaggcttc caggaagtgt tccatggtgc
    51001 ccgcgccacc ggaggcaatc agcgggacgt ggcaaacttc acgcactttt ttcagctgct
    51061 tgaggtcgta accgttacgc acgccgtcct gattcatcat gttgaggacg atttctccgg
    51121 cgccgcgttt ttgcacctcc tctacccagt cgagcgtttc ccattgagtg acgcgggtgc
    51181 ggctttcatc gccggtatat tgatttacat gatatttacc ggtttcggcg tcgtaccagg
    51241 tatcaatacc aaccacaata cactgcacgc caaagcgatc ggccaggcga gtaattaatg
    51301 ttgggtctgc cagcgcaggg gagttgatgg aaattttatc cgcgccaaag gaaagaattt
    51361 tcgcggcatc ttccagagac ttaatcccac ccgccacaca aaacggaatg tcgatcactt
    51421 ccgccacgcg agatacccag cttttatcta ccacacggcc atcgctggaa gcggtgatat
    51481 cgtagaacac cagttcatct gcaccttctt cggcgtagcg ttttgccagt ggcacgatat
    51541 cgccaatgat ttcatggttg cgaaactgta cgcctttcac cacctgacca tcacgaacgt
    51601 cgagacacgg gattatgcgt tttgccagca tgcgatggcc tccttcacgg tgaatttacc
    51661 ttccagtaat gcacgaccaa ctattacgcc gcgcacgcca gtgccacgta gggcagcaac
    51721 atcgttaatg tcgccaatgc cgccggagga ctgaaacgcc acttgcggat atctggcaca
    51781 cacttcttca tataaagaga cgttagatcc cgccagcgtg ccgtcgcgcg agatatcggt
    51841 acacaacaca tgtttcaggc cgacgggcag ataggtttcc accagttgtt ccagtgaaac
    51901 gcccgagttc tcttgccagc cgctgactgc cacttgcttg ttgccttgct cgtcaatacg
    51961 gacatccagt gccagcacca gcgcatcggc accgaaacgt tcaaaccagc ctttcactac
    52021 gtccggtgat ttcactgcgg tggaaccgac caccacgcgc gcaacgccag cttccagtaa
    52081 cgccgccacg tcttcttcac tacgaacacc gccgccaacc tgcaccggga cgttaacgcc
    52141 cgcgaccagg gttttaatca gcgggatttg acgcttagcc ggatcttttg ccccggtcag
    52201 atccaccagg tgcaacactt cggcaccttg cgcagcgtaa tcctgtaagc gcggcagcgg
    52261 gtcgttaccg taatcgcgtt gtttgccgta atcgccctga tggagacgca ccacggtgcc
    52321 gtcgattaaa tctaatgccg gaataatcat cacatctcca ggaagttttt cagcaactta
    52381 gcgccagcgg caccagaacg ctccgggtgg aactgcacgc cgtagaagtt atctttttgt
    52441 accgccgcgg tgaacggttc gccgtaatta cactgggcga tggtccacgg attgactggc
    52501 attgcgtagc tgtgaacaaa gtaaaagtac gcgccgtctt caatcccctg aaacaggcgg
    52561 ttgcctgcct gcggataaac gcggttccag cccatatgtg gcagtggcag accaaagtcg
    52621 gtcattttcg gcacgtcttc gtcgatgatg cccagcaagt cgacgccatt gctctcttcg
    52681 ctgtgtcgtc ccaggagttg catccctaag cagatgccca gcaccggttg ggtacaggct
    52741 ttgatgagat caaacagctc gcgctcacgc acctgatcca tcgctgcttg tgcggtgccg
    52801 acgccgggta aaaacagttt atcggccagc aacacaacgt ccgggtcacg gctgactttg
    52861 ggttcataac cgtggcgcgc aatggcagac ttcacagagt tcaggttggc gcagccagta
    52921 tcaaggatca ccacgttcat tacagcactc ctttcgacga gggcagggtg tcgccttcga
    52981 cgcggatggc ctggcgcagg gtacgaccaa aggctttgaa caggctttct acacggtgat
    53041 gatcgttttt acctttggtt ttcaggtgta gtgtcacgcc catggtgtaa gagagcgaac
    53101 ggaagaagtg ttcgatcatt tcagtgctga gatcgcccac acgctggtag gtaaactcgg
    53161 ctttatattc caggtgcggg cgaccagaga tatccagtgc gcagcgggca aggcattcgt
    53221 ccatcggcag cacaaaacca aagcggcaaa taccgcgttt gtcgccgagg gcaattttta
    53281 acgcttcgcc cagcgccagg ccggtatctt cgacggtgtg gtgatcgtcg atatagaggt
    53341 cgcctttgac attgatttcc atgcggaaac cgccgtgggt ggcgatctga tccagcatgt
    53401 gatcaaagaa gccaacgccg gtgttaatct tgctgccacc ttcgcgatcc agccacacct
    53461 ggacgtcaat ctgcgtctct ttggtgttgc gcactacatg ggcgtaacgg tctcgtttag
    53521 tgagttgctc gccaatcatt ggccagttca gggtttcgcg gtcgtagcgt aaaccattaa
    53581 tgcccatgtt ttccgccagt tgaatgtcgg tcgcgcgatc gccaattaca taactgttgg
    53641 cacgatccat cgcttgctca gccagataac cctccaccag ttttactttc ggcttacggc
    53701 agtcgcactc atcggcgggc aggtgcggac aaatcagcac ttcatcaaac tgcacgcctt
    53761 gcgaggtgaa gatctgcatc atcaggttgt gcgggccatc aaaatccgct tgcgggaaac
    53821 tttgtgttcc cagaccatcc tgattagtga tcatcaccag cttgtagccc gctttttgca
    53881 gcttcagcaa ttccgggatc acgcccggtt caaaggcgag tttgtcaaaa cggtccacct
    53941 gaaaatcact cggcggttcg ctaatcaggg ttccatcgcg atcgataaaa agatacttct
    54001 gactcatcaa acttgctccg cacgtaaggc gtcaatgacg cgctggcttt cttcacgggt
    54061 tccgacggta attcgcaggc agccgcttaa agagggttgt ttattctgat cacgtaagat
    54121 aatgccctga tcccacaaag atttaaacac tgcactggag gctttaaagc gcgctagaat
    54181 atagttggtt tcagagtcga aaacctgctc cacgcagggg atctctttca gtgcggcaat
    54241 caggtattcg cgttctgtaa taatttgcgc cacccgttcg cgcatggcga cgattccctg
    54301 cgggcttaac gcctgtgcgg caatgtcggc aaccggcgtc gagagcgggt agggggggat
    54361 cactttcatc agcaggttga tgacttcttc gtttgccagc gtaaatccgc aacgaagccc
    54421 cgccaacgcg aaggctttcg aaagggtacg caaaatcgcc aggtgcggat attccgccag
    54481 ccagccagct aacgatgcct gcgggcaaaa ctcgatataa gcttcatcgg caaccacaat
    54541 cgccttaccg cgggttagct ccagcagggg gcgaaaatcc tgcggattga tcagttgccc
    54601 ggtggggttg ttggggctgc aaacatagac cacttttacg ccgtccagct tgtcggaaat
    54661 gccctgtaag tccagttgcc agttttccag cgtcggcact gtgcggcatt cgacaccaat
    54721 ggtttcagcg ctgacgctgt acatgccgta cgttggcggg cagtagagga tggcgtcttt
    54781 acccggttcg caaaaagcgc ggatcagcag ttcaatacct tcgtccgcgc cacggctgac
    54841 cagcacctgt tccggtttta cgcctgcata ttgcgcgtaa ttttcaatca ccgcttttgg
    54901 ctggcattcc gggtagcggt tgagcgtttg ctgagtaagc tgaaactcca cggctgtggg
    54961 atattcgttg gcgttcagcc agacgtcgcc gttaccgccc agacgacgcg ccgactgata
    55021 cggcgtcagg ttgcggacgt tttcacgcgc taaatcagta atagtcacgg tgctcatgct
    55081 tgctccttaa ggacgttaac acgcaaagta acggcatttt tgtgggcggt caggcgctcg
    55141 gcggcggcca gtgtttcaat ggtcgaagcc agcgcggaga aaccttcttt cgacagttcc
    55201 tgtacggtca tgcgtttctg gaaatctgcc agcccgaggc tggaacaggt ggcggtgtaa
    55261 ccgtaagtcg gcagcacgtg gttggtgccg gaggcgtagt cgcctgccga ttccggtgac
    55321 cagtcaccaa gaaataccga accggcgctg gtgatgccat cgaccagttc gcgggcgttg
    55381 cgggtctgaa tgatcaggtg ctccgggccg tactggttgg agatctccac gcactgcgct
    55441 aaatctttag tcacgatcag gcggctggcg ttcagtgcct ggcgggtggt ttcggcacgc
    55501 ggcagttctg ccagttggcg ttcgacggcc tcggcaacgc gacgcgccat atcagcatcg
    55561 ggggtcagta aaattacctg tgagtcaggg ccgtgttcag cctgagaaag caaatcagaa
    55621 gccacaaaat ccggcgtagc gccgctgtca gcaatcacca gcacttccga cgggcctgcg
    55681 ggcatatcga tcgccgcacc gtccagacgc tggcttacct ggcgttttgc ttcggtgaca
    55741 aaggcgttac ccggcccgaa gattttgtcc actttcggca cagattccgt accaaacgcc
    55801 agcgcggcaa tggcctgtgc gccgccgacg ttaaacacgt cctgcacatc gcacagctgc
    55861 gccacataaa gaatttcatc ggcaatcggc ggcggtgagc acagcaccac ttttttacag
    55921 cccgcaatac gcgccggagt tgccagcatt aataccgttg agaagagcgg ggcggagccg
    55981 cccggaatat acaaacccac tgaagccacc gggcgcgtga cctgctggca acgcacgcct
    56041 ggctgcgttt ctacatctac cggcggcagt ttttgcgcag tgtggaaggt ttcaatactc
    56101 tttactgcca ccgccatcgc ctgttttagc tcgtcgctca ggcgttcgct ggcggcggcg
    56161 atctcatcag cagacacctt cagcgcggta accgtggttt tatcaaactt cgcgctgtat
    56221 tcacgcaggg cctcatcgcc acgcgttttc acgttatcga gaatatcgtt aacggtgcgg
    56281 gtaatgcttt cagaagcgga gatcgccggg cgtattaaca gctggcgttg ttgctccgca
    56341 gtacagctat tccagtcaat gattgtgtta aagctcatgg cgatcactcc atcatcttct
    56401 caatcggcag gaccaggatt gaactggcac ccagtgcttt cagtttttcc atcgtttccc
    56461 agaacagggt ttcgctactc accatgtgca tcgctacgcg ctgttgatca cccgccagcg
    56521 gcagaatggt tgggcgttcg gcacctggca gcagggcgat gacttcatcc aggcgttcgg
    56581 tcggtgcgtg catcatgatg tattttgatt cgcgcgcctg gatcacaccc tgaatacggg
    56641 tcagtagctt gtcgatcagt tgctgtttag attcttccat ttcgccatcg cgctggatca
    56701 ggcaggcttt cgagcggtag ataacttcga cttcgcgcag gccgttagct tccagcgtgg
    56761 caccggtgga aaccagatcg caaatcgcat ccgccagccc ggcacgcggc gcaacttcga
    56821 cagaaccgtt cagtaagcag gatttaaaag agataccttt ctggtcgaga taacgcttga
    56881 gcaggtgagg ataagaggtg gcgatacgtt taccgtttaa ggagagcgga ccgtcccagg
    56941 cttcatcaac cggcgttgcc agcgaaaggc ggcagccgcc gaaatccaga cgacgcaggg
    57001 taaagtagcg cggatcttca ccctgggcgc ggcggttaag cagctcttct tccagcacgt
    57061 tttcgccgat aatcccaagg tctaccacgc cgtccattac cagaccggga atgtcatcgt
    57121 cacgcacgcg cagaatatca atcggcatgt tttctgccat cgcgatcagg cgctgggtgt
    57181 gaagattgat tttaatgcca cagcgcgcca gtaattcgcg tgagtcatca cttaaacggc
    57241 cggatttctg catagctatg cgtaaacgag tgttgtctgt cattttctta ttcctcttta
    57301 aacctgtctg aaccgttaac aaccgcgcaa taaaaaagcc cccggaaggt gatcttccgg
    57361 gggctttctc atgcgttcat gcaccactgg aagatctgaa tgtcttccag cacacatcgc
    57421 ctgaaagact agtcaggatg atggtgatga tggtggtgtt taaattgaac gcgtgtcata
    57481 aaaatctctg tgaatgttta ttcaactgat gtcttttaac ctaaaccact ttcacgttag
    57541 aaagcaaggg cttttttata tattttatgg cgatggattt gtttaatgaa tgagcgggga
    57601 aaggaggggg ctaagaggac tgtcctctaa cttgacagca ccaaaaagag caaagtctga
    57661 tttttcgtct cgctggcaat gtcttgatct acaaactaat taataaatag ttaattaaca
    57721 cctatcattg tacaatgaac tgtacaaaat aggagattta catgcgtaca attagctaca
    57781 gcgaagcgcg tcagaatttg tcggcaacaa tgatgaaagc cgttgaagat catgccccga
    57841 tcctcattac tcgtcagaat ggagaggctt gtgttctgat gtcactcgaa gaatacaact
    57901 cgctggaaga gacggcttat ctactgcgtt cccccgctaa cgcccggaga ttgatggact
    57961 caatcgatag cctgaaatca ggcaaaggaa cggaaaagga cattattgag tgaaactaat
    58021 ctggtctgag gaatcatggg atgattatct gtactggcag gaaacagata agcgaattgt
    58081 taaaaagatc aatgaactta tcaaagatac ccgcagaaca ccatttgaag gtaaggggaa
    58141 accagaaccc ctgaaacata atttgtcagg tttctggtcc cgacgcatta cagaggagca
    58201 ccgtctggta tacgcggtta ctgatgattc actgctcatt gcagcatgtc gttatcatta
    58261 ttgaacagat tcattcatag aatgaattgt cagttttgat acgctagcgt agccttatag
    58321 atctaaggtg tatcaggaga taacggatga aaaaggtcgc aattgtcggg ttagggtggt
    58381 taggtatgcc gctggcgatg tcactttcag cgcgaggctg gcaagtcacc gggagtaaaa
    58441 ccacacaaga tggtgtcgaa gcggcccgaa tgagtggcat tgatagctat ctgcttcgca
    58501 tggagcctga gttagtttgc gattctgacg atctggatgc ccttatggat gccgatgcgc
    58561 tggtcattac gcttccggca cgtcgtagcg gccccggcga tgagttctat ttacaagcgg
    58621 tacaagagtt agtggatagc gcgctggccc atcgtattcc ccgaattatt tttaccagct
    58681 caacatctgt ctatggcgac gcgcaaggca cggttaaaga aactaccccg cgtaatccgg
    58741 taaccaacag tggacgagta ttagaagaac tcgaagactg gctgcacaat ttacccggta
    58801 cttcggtcga tattctgcgt cttgcgggcc tggtcggacc gggacgtcat ccgggacgct
    58861 tctttgctgg aaaaaccgcc cctgatggtg aacatggtgt taatttagtc catttagaag
    58921 atgttattgg cgctatcact ctgttgttac aggcacctaa aggcggacac atctataata
    58981 tatgtgcgcc agctcacact gcgcgtaatg ttttctatcc gcagatggcc cgtttactgg
    59041 ggctgcaacc tccgcagttc agaaatagtc tggacagcgg caaaggtagg attattgatg
    59101 gcagtcggat ttgtaatgaa ctgggatttg aataccagta tcccgatccg ctggtaatgc
    59161 cgctggagta aacgatcacg ccagcaggac gccgcatgca ctgcaagggg gcgtgtatga
    59221 aaccactgct ggatgtgttg atgatcctcg atgccataga aaaagagggc agttttgcgg
    59281 cggcgtcagc caaactctac aagacgccgt ccgctctgag ttacaccgtt cacaagctgg
    59341 aaagcgacct aaatattcaa ttgcttgatc gtagcggaca ccgcgctaaa ttcacccgca
    59401 ccggaaaaat gttattagag aaagggcggg aagttctgca taccgtgcga gaactggaaa
    59461 agcaggcgat caaactgcat gaaggctggg aaaacgagct ggtgattggc gtggacgaca
    59521 cgtttccttt ttctctgctt gcgcctctca ttgaagcttt ttatcaacat catagcgtga
    59581 cgcgcctgaa gtttatcaat ggcgtactcg gtggttcctg ggatgccctg actcaggggc
    59641 gagcggatat tatcgttggc gcgatgcatg agccaccatc ttccagtgag tttggtttct
    59701 cgcggttggg cgatcttgaa caagtctttg cggtcgcgcc tcatcatccg ttagcccagg
    59761 aagaagaacc gttaaaccgt cgtatcatta aacgatatcg ggcgattgtg gtgggggaca
    59821 ctgcgcaggc aggcgcttct acggcctcac aacttctcga cgaacaagaa gccattaccg
    59881 ttttcgattt taaaaccaaa ctggagctgc aaattagcgg ccttggctgc ggctatttac
    59941 cacggtatct ggcgcaacgt tttctcgata gtggcgcgtt aatcgagaag aaagtggtcg
    60001 cccaaactct ctttgaacct gtctggattg gctggaatga acagaccgca gggcttgcca
    60061 gcggctggtg gcgagatgaa attttagcaa atagtgcgat cgccggtgtt tatgcaaaat
    60121 ctgatgacgg aaaatcagcc atttaaagaa aaattattat gacaagcctc tcattctctt
    60181 gtcatttccc ccccatttag gcacaatgcg ccgctgtcaa aaaatgacta aaaaccgacg
    60241 tttaattagc gtcggttatt ttttgcttca aaccaatcat tcataccaag aggccgggct
    60301 tcgtaccgga tagatattta ctaaaaatcg acagttgttg tcgctgagga atccaaaaaa
    60361 atggggcaat tttttgctta cgcgacggtt atcaccgtaa aggagaataa ccatgtcgca
    60421 taacgttact ccaaacacct ctcgcgtgga attgcgtaaa acgcttacgt tagttccggt
    60481 tgtaatgatg ggtctagcct atatgcagcc gatgacgctg tttgatacat ttggtatcgt
    60541 ttcaggcctc acggatggtc atgtgccgac agcctatgcg ttcgcattga ttgcgatcct
    60601 gtttacggct ctgagctacg ggaagctggt tcgccgctat ccttctgctg gctctgcata
    60661 cacttacgcc cagaaatcca ttagcccgac tgtcggcttt atggtgggtt ggtcttctct
    60721 gctcgactat ctgttcgcgc cgatgatcaa cattctgctg gcgaaaatct attttgaagc
    60781 tctggtgcct tccattccat cgtggatgtt tgtggtggcg ctggtggcct ttatgaccgc
    60841 ctttaacctg cgtagtctga aatccgtagc gaacttcaac actgtaatcg tcgtgttgca
    60901 ggtagtgctg atcgcggtga ttctgggcat ggttgtttat ggcgtatttg aaggtgaagg
    60961 cgctggtacg ctggcgagca cgcgtccgtt ctggtctggc gatgcgcatg ttatcccgat
    61021 gattaccggg gcgacaatcc tgtgcttctc ctttaccggc tttgacggca tcagtaatct
    61081 gtcggaagaa accaaagatg cagagcgcgt gatcccgcgt gcgattttcc tgaccgcgct
    61141 gattggcggc atgatcttca tctttgcaac ttacttcctg cagctgtact tcccggatat
    61201 ctctcgcttt aaagatccgg atgcgtcaca gcctgaaatc atgctgtacg ttgcgggtaa
    61261 agcgttccag gttggcgcgc tgatcttctc caccattacc gtactggcgt ccggtatggc
    61321 ggcgcacgca ggcgtagcgc gtctgatgta cgtaatgggt cgtgacggcg tgttcccgaa
    61381 aagcttcttc ggttatgtac acccgaaatg gcgtactccg gcgatgaaca tcgtcctggt
    61441 tggcgcgatt gccctgctgg caatcaactt cgacctggta atggcaacgg cgctgattaa
    61501 ctttggtgcg ctggtggcgt tcacctttgt taacctgtcg gtcatctcgc agttctggat
    61561 ctgtgagaag cgtaacaaga cgctgaaaga tcacttccag tatctgttcc tgccgatgtg
    61621 tggtgcgctg accgttggtg cgctgtgggt taacctggaa gaaagctcaa tggttctggg
    61681 tctgatctgg gctgctatcg gtctgattta cctggcttgc gtcaccaaga gcttccgtaa
    61741 tccggttccg cagtacgaag acgtagcata atcatctttt ctaataataa acaggagtcg
    61801 aatgaacatt tggctccggt ttttttattc ttttttatcg aaatcctcat taataaccat
    61861 ttgttctaat ttagagaatt tggtt
//
LOCUS       DADLSE010000008        56200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLSE010000008 DADLSE010000000
VERSION     DADLSE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.008"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1563)
                     /gene="cirA"
                     /locus_tag="JN692_000502"
     CDS             complement(<1..1563)
                     /gene="cirA"
                     /locus_tag="JN692_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HAY7262902.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGIKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYY"
     gene            complement(1855..3324)
                     /gene="lysP"
                     /locus_tag="JN692_000503"
     CDS             complement(1855..3324)
                     /gene="lysP"
                     /locus_tag="JN692_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7262903.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(3530..4411)
                     /locus_tag="JN692_000504"
     CDS             complement(3530..4411)
                     /locus_tag="JN692_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262904.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGWLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            4510..5559
                     /locus_tag="JN692_000505"
     CDS             4510..5559
                     /locus_tag="JN692_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7262905.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSTLKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5633..6490
                     /gene="nfo"
                     /locus_tag="JN692_000506"
     CDS             5633..6490
                     /gene="nfo"
                     /locus_tag="JN692_000506"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7262906.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            6493..7582
                     /locus_tag="JN692_000507"
                     /pseudo
     CDS             6493..7582
                     /locus_tag="JN692_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(7638..8888)
                     /locus_tag="JN692_000508"
     CDS             complement(7638..8888)
                     /locus_tag="JN692_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7262907.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(8988..9929)
                     /gene="rihB"
                     /locus_tag="JN692_000509"
     CDS             complement(8988..9929)
                     /gene="rihB"
                     /locus_tag="JN692_000509"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7262908.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVVGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10060..10758
                     /gene="yeiL"
                     /locus_tag="JN692_000510"
     CDS             10060..10758
                     /gene="yeiL"
                     /locus_tag="JN692_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7262909.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKHLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(10829..12079)
                     /locus_tag="JN692_000511"
     CDS             complement(10829..12079)
                     /locus_tag="JN692_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7262910.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAILPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12173..13111)
                     /locus_tag="JN692_000512"
     CDS             complement(12173..13111)
                     /locus_tag="JN692_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7262911.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(13099..14040)
                     /locus_tag="JN692_000513"
     CDS             complement(13099..14040)
                     /locus_tag="JN692_000513"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7262912.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGKNGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(14464..16155)
                     /gene="fruA"
                     /locus_tag="JN692_000514"
     CDS             complement(14464..16155)
                     /gene="fruA"
                     /locus_tag="JN692_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7262913.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCADMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(16172..17110)
                     /gene="fruK"
                     /locus_tag="JN692_000515"
     CDS             complement(16172..17110)
                     /gene="fruK"
                     /locus_tag="JN692_000515"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7262914.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(17110..18240)
                     /locus_tag="JN692_000516"
     CDS             complement(17110..18240)
                     /locus_tag="JN692_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7262915.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            18608..19819
                     /gene="setB"
                     /locus_tag="JN692_000517"
     CDS             18608..19819
                     /gene="setB"
                     /locus_tag="JN692_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7262916.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALALALALALALAREHADKTGREAVMFSSFLRA
                     QVSLAWVIGPPLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTV
                     EAPRRNRRDTLLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEI
                     PTMLIAGYFAKRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGG
                     IGMLYFQDLMPGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIA
                     TLFCLLRIKDV"
     gene            complement(19816..20070)
                     /locus_tag="JN692_000518"
     CDS             complement(19816..20070)
                     /locus_tag="JN692_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7262917.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            20225..20797
                     /gene="yeiP"
                     /locus_tag="JN692_000519"
     CDS             20225..20797
                     /gene="yeiP"
                     /locus_tag="JN692_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7262918.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            21020..22486
                     /locus_tag="JN692_000520"
                     /pseudo
     CDS             21020..22486
                     /locus_tag="JN692_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            22604..23590
                     /locus_tag="JN692_000521"
     CDS             22604..23590
                     /locus_tag="JN692_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7262919.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            23629..24342
                     /gene="lpxT"
                     /locus_tag="JN692_000522"
     CDS             23629..24342
                     /gene="lpxT"
                     /locus_tag="JN692_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7262920.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALTIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            24754..25320
                     /gene="mepS"
                     /locus_tag="JN692_000523"
     CDS             24754..25320
                     /gene="mepS"
                     /locus_tag="JN692_000523"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7262921.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            25501..27057
                     /locus_tag="JN692_000524"
     CDS             25501..27057
                     /locus_tag="JN692_000524"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7262922.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            27139..28953
                     /locus_tag="JN692_000525"
     CDS             27139..28953
                     /locus_tag="JN692_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7262923.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDCLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            28954..30048
                     /locus_tag="JN692_000526"
     CDS             28954..30048
                     /locus_tag="JN692_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7262924.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     DNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            30048..31073
                     /locus_tag="JN692_000527"
     CDS             30048..31073
                     /locus_tag="JN692_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7262925.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            31075..32664
                     /gene="yejF"
                     /locus_tag="JN692_000528"
     CDS             31075..32664
                     /gene="yejF"
                     /locus_tag="JN692_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7262926.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(32668..33012)
                     /locus_tag="JN692_000529"
     CDS             complement(32668..33012)
                     /locus_tag="JN692_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262927.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(33345..34535)
                     /gene="bcr"
                     /locus_tag="JN692_000530"
     CDS             complement(33345..34535)
                     /gene="bcr"
                     /locus_tag="JN692_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7262928.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(34563..35258)
                     /gene="rsuA"
                     /locus_tag="JN692_000531"
     CDS             complement(34563..35258)
                     /gene="rsuA"
                     /locus_tag="JN692_000531"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7262929.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            35408..37168
                     /locus_tag="JN692_000532"
     CDS             35408..37168
                     /locus_tag="JN692_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7262930.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKENHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            37293..37577
                     /gene="rplY"
                     /locus_tag="JN692_000533"
     CDS             37293..37577
                     /gene="rplY"
                     /locus_tag="JN692_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7262931.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(37716..38723)
                     /gene="yejK"
                     /locus_tag="JN692_000534"
     CDS             complement(37716..38723)
                     /gene="yejK"
                     /locus_tag="JN692_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7262932.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            38905..39132
                     /locus_tag="JN692_000535"
     CDS             38905..39132
                     /locus_tag="JN692_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7262933.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            39152..40912
                     /gene="pbgA"
                     /locus_tag="JN692_000536"
     CDS             39152..40912
                     /gene="pbgA"
                     /locus_tag="JN692_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7262934.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            40987..41063
                     /locus_tag="JN692_000537"
     tRNA            40987..41063
                     /locus_tag="JN692_000537"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41021..41023,aa:Pro,seq:ggg)
     gene            complement(41166..43757)
                     /gene="yejO"
                     /locus_tag="JN692_000538"
     CDS             complement(41166..43757)
                     /gene="yejO"
                     /locus_tag="JN692_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7262935.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYDETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGNITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGNMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            44077..44724
                     /gene="narP"
                     /locus_tag="JN692_000539"
     CDS             44077..44724
                     /gene="narP"
                     /locus_tag="JN692_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7262936.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     ILHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(44935..45987)
                     /locus_tag="JN692_000540"
     CDS             complement(44935..45987)
                     /locus_tag="JN692_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7262937.1"
                     /translation="MRFLLGVLMLMIFGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(45984..46541)
                     /gene="dsbE"
                     /locus_tag="JN692_000541"
     CDS             complement(45984..46541)
                     /gene="dsbE"
                     /locus_tag="JN692_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7262938.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(46538..48481)
                     /locus_tag="JN692_000542"
     CDS             complement(46538..48481)
                     /locus_tag="JN692_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7262939.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFTIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(48478..48957)
                     /gene="ccmE"
                     /locus_tag="JN692_000543"
     CDS             complement(48478..48957)
                     /gene="ccmE"
                     /locus_tag="JN692_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7262940.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(48954..49163)
                     /gene="ccmD"
                     /locus_tag="JN692_000544"
     CDS             complement(48954..49163)
                     /gene="ccmD"
                     /locus_tag="JN692_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7262941.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(49160..49897)
                     /gene="ccmC"
                     /locus_tag="JN692_000545"
     CDS             complement(49160..49897)
                     /gene="ccmC"
                     /locus_tag="JN692_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7262942.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGLA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(49939..50601)
                     /gene="ccmB"
                     /locus_tag="JN692_000546"
     CDS             complement(49939..50601)
                     /gene="ccmB"
                     /locus_tag="JN692_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7262943.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(50598..51215)
                     /gene="ccmA"
                     /locus_tag="JN692_000547"
     CDS             complement(50598..51215)
                     /gene="ccmA"
                     /locus_tag="JN692_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7262944.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(51234..51836)
                     /gene="napC"
                     /locus_tag="JN692_000548"
     CDS             complement(51234..51836)
                     /gene="napC"
                     /locus_tag="JN692_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7262945.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(51846..52295)
                     /gene="napB"
                     /locus_tag="JN692_000549"
     CDS             complement(51846..52295)
                     /gene="napB"
                     /locus_tag="JN692_000549"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7262946.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(52292..53155)
                     /gene="napH"
                     /locus_tag="JN692_000550"
     CDS             complement(52292..53155)
                     /gene="napH"
                     /locus_tag="JN692_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7262947.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(53142..53837)
                     /gene="napG"
                     /locus_tag="JN692_000551"
     CDS             complement(53142..53837)
                     /gene="napG"
                     /locus_tag="JN692_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7262948.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(53844..>56200)
                     /gene="napA"
                     /locus_tag="JN692_000552"
     CDS             complement(53844..>56200)
                     /gene="napA"
                     /locus_tag="JN692_000552"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7262949.1"
                     /translation="PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLP
                     KIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMF
                     GSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIE
                     QADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQ
                     SDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDA
                     SEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHT
                     RGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNE
                     KHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEER
                     MPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAP
                     GEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELA
                     EDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQW
                     RYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWH
                     TGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNR
                     PPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
BASE COUNT        13763 a        15188 c        13763 g        13486 t
ORIGIN      
        1 gtagtagcta aataccggta tgcgccgtcc gttagcgccc gtctcaaaac caacaaagtt
       61 ttggtagccc ggtgcagcgt tgacgtcaga cgtacggctg atgctgatac gatctttcac
      121 atcgttacgg aaaacgataa cgctggattc aaccccttcc agccagcctt cttcacccat
      181 gtagtaaagc cccagctccc aactttcgct ggtttctggt ttcagatccg ggctacccac
      241 aatcttacat gcgccacggc aggaattgct cgtccagtca gggctaagtt gcaacagaga
      301 aggtgcttta aatgccgtcg cccagccccc tttcaccgtt acggtgtcgg tggcgttata
      361 aaccaggtag gcacgcggac tccagtgttc accgtaggtt tcgtgatcgt ccatacgcac
      421 gccggtcgtc agcgccagcg gctcgaagat ccgccattca tcttccacaa acagcgcgta
      481 ctggctggca gacgttttgg agctggttcc cccggtcagg ttcaccgcat cgctaagttt
      541 gtcgtgacgc cattcaccgc caaccgtgag aaactgatta atcgccgtca gcggcaacgt
      601 gtatttgccg tcgaccgtat tgctttcgga agttatcggg ctgctgttgc cagggttttt
      661 gttctcgact ttctcaccgt agtatttcag ttcgctggtg ccgtaatccc aacgcccatt
      721 atggctgacg gagtagttct ggcgttccag gcggtttttg tccagcgagt cggaatcacg
      781 atcctgacgg tcgaaaccgt atccggcagt aaaatcgtga ttttgattcg gtgtccaggc
      841 aaattcgaca ttgccgtcgc ggctggagaa tccttcaata cgcggcgttt ctccggtatc
      901 ggtggtcgtt gagttttgcg ggtcatcctt ttcacgtttt gccaggctgc cgtaagcttt
      961 tattcccagc acaccatcaa ttaatggtcc actggtaaag aactgaccgt tataggtatc
     1021 accgcgatcg cgatgttcct gaatggtggt atcgacggta acggtacccg accatttctg
     1081 accgattttt ttggtgatga tattcactac accgccgagc gcatcggagc cgtatagcga
     1141 cgacatcggg ccacgaacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag
     1201 atcgaaatca ttgtggcgga agacggcatt gcgggagtta acgcgtttac cgtcgacaag
     1261 aatcagggtg tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc
     1321 gttcgtcagt tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt
     1381 tcgctgcagg tcttcctggg taatgacgct gatactggcg ggtgcatctt taagattttg
     1441 ttccacggaa gatgcagtga caaccatcgt ttcgccatca tcatcgaccg ctaacacagg
     1501 ccatgcacaa gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa
     1561 cattccatat ctccatgagg taactacgaa aataaaatgg gtatcgctca catcttcttt
     1621 acgtcccctt gcgtacggca gcatcacggt ggacttattt gtatgcaggt gatcatccca
     1681 gaaagccgga acagcctctg atttgtcagc cttttttgat tgcgtcgtaa cgataatgca
     1741 aacgataaca attatcaatc caaatgttaa attttatatc cgcatgattt gtcagggaaa
     1801 tggaattgaa aaagccctct cggttgagag ggcttagcaa ggaagggagg aaacttattt
     1861 cttatcgttc tgcgggaact tcatttcgct gtagcgtacg aagtgagttc ctttaatcag
     1921 cttgtagccg aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc
     1981 gccccagtca atggtatctt tcaggaacgc ttcgtagttc tggcccaaag tgataatcag
     2041 acacagaatg aatgcgaaga tcggccccag tgggaagaaa cctgaacggt acggcagatc
     2101 gttaatgtcg tgtccctgca atacgtaacc gcgacggaag cgatagtggc taatggcaat
     2161 ccccagccag gcgataaaac ccgtcatccc ggaggtgttc agcagccaca ggtataccgt
     2221 ctggttgcca aacatggagg tcaggaagca caaaccggca atcaccgtcg tcgcatacag
     2281 cgcattacgc ggcacgccac cacgcgacaa tttagcgaaa atgcgcggcg ctttaccgtc
     2341 acacgccagg gtgtacagca tacgagtaga cgcatacata ccggagttac ctgctgacag
     2401 caccgccgtc agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg
     2461 gaacaccagg gtgaacggac tgacgctgat gtctttaaca tcgttacgca gcaggctcgg
     2521 atcggtatac ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat
     2581 tcgccagaat acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc
     2641 tgcagcaata ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc
     2701 gatcatcgcc gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc
     2761 cgcaggctgc gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac
     2821 aataactgtc gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccgcgaac
     2881 agagatgtag ttcaacagga agataacgcc gaggaacaac gcactccaga tccagcccgg
     2941 tgtatccggg aaccaccagc tcatgaccag ctgtgctgca accaggtcaa cggcaatagt
     3001 caccgcccag ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata
     3061 gttctgaccg taagtggcaa acgaaccgga aaccggcata tatgcagcca attcaccgag
     3121 actggtcatc aggaagtaaa ccatcagtcc aatcagcata tacgagagca atgccccgcc
     3181 cggacctgcc tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc
     3241 gccaatggca atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc
     3301 tgtagtttta gtttcggaac ccatacaaaa atgctatcca tcttaaaaat gatggcgcga
     3361 ttgtagcaga cgattggcaa tccttccggc agaaatacac ggttataaga caccttcatg
     3421 atcgcccagg gattataagt aaagcagcca atctcttttc tggcgaatgc atgctgaagg
     3481 cgctgcgtca cgaaatcgac acagcaccag catgttcttg tacagcaact taacgcggca
     3541 catttgcggg atcgcaatag tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc
     3601 gatgatgtat acgccacaac gtacgcatca ggcgcggcag agggaccgca acttcactta
     3661 atgtgcctgc ctgcaattga tcttcaatca cacgtcgcga caggcaacta attcccaacc
     3721 catgacgcac cgcatgtttg attgcctcgg agttacctaa ttccatcgcc atctcaaact
     3781 tcggtaaatg tgacagcaac agataatcga caatctcccg cgtgccggaa ccgcgttcac
     3841 gcaggatcca cggcgcagcg gccagctgtt ctaaggtgac cggaccacga gccaacggcg
     3901 aagtcggtgc ggcgaaaacc accagctcgt cttccagcca cggttcagaa atgatttcag
     3961 tgctgtggca cggtccttca ataaagccaa tatcaacgcg gaaatccagc accgcttgca
     4021 tcacgtcctg gctgttccca acgctaagtt caatcggcaa ctgcggataa tcatggcgat
     4081 aacgggcgat aactgcaggc agaatgtagt taccgatggt actactggca tagatacgaa
     4141 tcgcgccgtt gtcttcgcga aacagttgtt cgatttcaac cgcctgttca agcagtgcca
     4201 acgctcgcgg atagagcagc cacccgtgtt cattaacaac cagtcttttc cccacgcgat
     4261 caaacagttg aacgccaagc tgcccttcca ggtcagtcaa ggctgcactc actgctgatt
     4321 gcgacaacgc cagcatcacc gacgcctggg tggttgatcc acttttcaat acttctgcaa
     4381 aaacttccaa ctgccggagg gtgatgtgca tagtcgctta ccacttataa agattaatta
     4441 taaatatata atcaatttta tttttaaacc agttagtcgt aaccttatgc ccggttaaag
     4501 gagagggtta tgacgaatat caccttacag aaacaacatc gtacactgtg gcattttatt
     4561 ccggggttag ccctgagtgc agttatcacc ggggtcgccc tgtggggtgg ttccattccc
     4621 gcggttgcgg gtgccgggtt tagtgccctc accctcgcaa tcttgttggg gatggtttta
     4681 ggcaacacca tctatccgca catctggaaa agctgtgacg gtggtgtgct gtttgccaag
     4741 caatatttat tacgtctggg tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt
     4801 gccgatgtcg gtatcagtgg gatcatcatt gacgtgctga cgctgtccag taccttcctg
     4861 ctggcttgct tcctggggca gaaagtgttt ggtctggata agcacaccag ttggttgatc
     4921 ggtgccggta gcagtatctg tggtgctgcc gcggtactgg cgactgagcc ggtagtgaaa
     4981 gcggaagcca gtaaagtaac cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg
     5041 attttcctct acccggcgat atatccgctg atgtctcaat ggtttagtcc ggaaactttc
     5101 ggtatctaca ttggttctac tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc
     5161 atcagcccgg atgcagaaaa cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg
     5221 gctccgttcc tcatcctgct ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc
     5281 gagaaaagca aaatcactat tccgtggttt gccatcttgt tcatcgtagt tgccatcttt
     5341 aactcgttcc acctgttacc gcagagcgtg gtgaacatgc tggtaacgct ggataccttc
     5401 ctgctggcaa tggcgatggc ggcgctgggt ctgaccactc acgtcagcac gctgaaaaaa
     5461 gctggggcga aacctctgct gatggcactg gtactgtttg cctggctgat tgttggtggt
     5521 ggtgcgatta actatgtgat tcaaagcgtc atcgcataaa ccactacttc ttgctcctgt
     5581 taacccgcta tcattaccgt tttcctccag cgggtttaac aggagtcctc gcatgaaata
     5641 cattggagcg cacgttagtg ctgctggcgg tctggcaaat gccgcaattc gcgccgccga
     5701 aatcgacgca accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc
     5761 gctcacgacg caaaccatcg atgaattcaa agccgcctgt gaaaaatatc actacacatc
     5821 ggcgcaaatt cttccccacg acagttatct gatcaacctc ggacatccgg tcactgaagc
     5881 tctggaaaaa tcgcgcgatg cctttataga tgaaatgcag cgttgcgaac aactggggct
     5941 ttctttgctc aacttccacc ctggcagcca tctgatgcag atttcagaag aggattgcct
     6001 tgcgcgtatt gccgaatcca tcaacattgc gctggataaa actcaaggtg taacagcggt
     6061 gattgaaaac accgccggtc agggcagtaa cttagggttt aaattcgaac atctcgcggc
     6121 gattatcgac ggcgtggaag ataaatcccg cgtcggcgtc tgcattgata cctgccatgc
     6181 tttcgctgcc gggtatgatt tgcgtactcc agccgaatgc gaaaaaacgt tcgctgattt
     6241 tgcccgtatt gtcggcttta agtatctgcg cgggatgcac cttaacgatg cgaaaagtac
     6301 ctttggcagc cgcgttgacc gccatcatag cctcggtgaa ggcaatatcg gtcatgatgc
     6361 gttccgctgg attatgcaga acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat
     6421 caacccggat atctgggcag aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc
     6481 ggtagcctga agatgaataa ccgggaaaag gagatccttg caattttacg gcgtaacccg
     6541 ctgattcagc agaacgaaat tgcggacatg ctgcaaatca gtcgttcgcg cgttgcggcg
     6601 catattatgg atttaatgcg caaaggccgg attaaaggca aaggttacat tctcaccgag
     6661 caggaatact gcgtagtggt ggggacaatc aatatggata ttcgcgggat ggcggatatc
     6721 cattacccgc aagcggcttc tcatcccggt acaattcatt gctcggcagg cggcgttgga
     6781 cgcaatatcg cccacaatct ggcgctgtta gcgccgtgac gtccatttgc tttcagtgat
     6841 tggcgatgac ttttatggcg aaatgctcct agaagaaacg cgccgtgccg gcgtgaatgt
     6901 ctccggctgc gttcgtttgc atggtcaaag cacatcgacg tatctggcaa ttgccaatcg
     6961 agacgatcaa accgtgctgg cgattaacga tacccatctg ctggaacagt tgacaccgca
     7021 actactgaac gggtcgcgcg atttacttcg tcatgcgggc gtggtactgg ctgattgcaa
     7081 cctgacagcc gaggcgctgg aatgggtctt taccctcgct gatgagatcc cggtgtttgt
     7141 cgataccgtt tcagaattca aagcgggcaa aatcaaacac tggctggcgc atattcacac
     7201 cctgaaaccc actttaccgg agctggaaat tttatgggga caggcgatca ccagcgatgc
     7261 tgaccgtaat gccgcagtga atgcattgca tcagcaaggt gttcagcaac tgtttgttta
     7321 tttgcccgat gagtcagttt atttcagcga aaaggatggt gaacaatttt tgctgactgt
     7381 gccagcgcat acgacggtag acagttttgg tgctgacgat ggttttatgg cgggtctggt
     7441 atatagcttt ctggagggaa acaatttccg tgacagcgcc cgttttgcga tggcctgcgc
     7501 ggcaatttca cgcgccagcg gcagcttaaa caaccctacc ctgtctgccg ataacgcgct
     7561 gtcattagtg ccaatggtgt aacaatgttg ccggattcga cgcttgaccg catctaatcc
     7621 ggctcataaa ttgcacacta cgctaaacca ataaagaacc cggcaatggt ggcactcatc
     7681 agattagaaa gtgtcgccgc cgccagcgcg cgtaaaccaa gctgggcgat ttccggcgca
     7741 cggtgtggcg caaccgcaga aaacgccccc accaccaccc cgatagaacc aaagttagcg
     7801 aaaccgcaca acgcgaagga aataatcgcc acggttttag catccagagt gccagccgtt
     7861 tgcagatagg gtgagaaatt gagataagcg acaaattcat ttattgccag tttctgtcca
     7921 atcaaactcc cggcaagatt tgcatcactc cagtcaacac ccattaccca cgcaagtggg
     7981 gccagcaggt aacctaaaat ggactccagc gaggcatgtt caaaaccaaa ccagccgcca
     8041 acgccgccga taataccgtt aattaacgca atgatggcga caaatgccat aacaactgtc
     8101 gctacaccgg cggcgatttt cagcccggtc attgcccctg tcgcagcggc ttcaataatg
     8161 ctttttggcg gtgtttcggt gaaagagagg ttattaaaag aaacctgcga agattccgta
     8221 gccgggctta acaggcgggc aaacaagatc ccgccaggga tcgccattaa cgatgccgcc
     8281 agcaaatatt ccacaggtac gcccagtgcg gcgtaaccaa tcattgtcga accagcaatc
     8341 gaggccatgc cactacaaat cgctgtaaat aattcattgc gattcagacg atcgataaag
     8401 ggtttgacga tcgccggaat ttcgttttgc ccgaggaaaa tggtggtgac cgcgacgaat
     8461 gactcgatct tgctgatatt taaagctttc tggaagatac cgccgagaat gcgaattaag
     8521 atccccatca caccgatgta gtagagaata ctcaccagtg cagtgacgaa gataattgcc
     8581 ggtaataccc tgaaaccaaa gataaatcct gcgccatcaa ataaggtatc cattttcggt
     8641 ccgaccagag aaccgaagat aaatgcgcta cctgcgtcgc tgtacgccat cactttgtgg
     8701 acgccaaaag cgactttttc tgcgacccaa cgccctggcg gtaaccaaag cataatcccg
     8761 ccaatcacga cctgtaacac taacgccgcg ccaacggtac gcaggctgat cttcttcttg
     8821 tttactgaca gtaaaaacgc aattgccagc agtaccacca ttcccagaac acttctcatg
     8881 acatccatag tgattatctc ttcatgtcat tttgggcgac ggctttctgc ccatcagaaa
     8941 tagccgggcc tgcatcttgc gatactgtcc cggcttgtta tcaacactta atgggttttg
     9001 atgtagccgc gcacgcactc ttcgactaag ccccagaacc agtcggtatc aatagtgatg
     9061 ccgactttgg tattagcggg cttgccaaga acgcccagtt cgtcgcagac cgtacgcccg
     9121 tagcaagggc cgctgttgac gtccacttcg acgtacatat cctgggtttt aatgccatca
     9181 gggttgatca gataaccaat gcaggtggcg tcatgcaccg ggccgccagc caggccgtag
     9241 tattcgaact gcgttttgag agtgaagttc atgatgtcgc tgaacagctc tccggcaggg
     9301 ccgcctaccc tttccatccg agcaattaca tccggggtgc aaacggtctg gttggtaaga
     9361 tcgaggccca tcatcactaa tggaacgccg gaggtgaaca ccacgcgggc ggcttccggg
     9421 tcggcaaaga tattgaactc ggcagatggg gtgaagttgc ctgtaccgta agcgccgccc
     9481 attagcacaa tttcacggat tttgggcagg attgcgggtt gcatacgcat tgccaccgcg
     9541 atatttgaaa gcggaccaac cggcaccaga gtgatatcgc catcgcttgc catcagggta
     9601 tcgatgatat atttcaccgc atgggtactt tctgcctggc gggtcagcgg ctcgaatacc
     9661 gggccatcca gtccggtttc accgtgaata ttatcggcaa cgatttgttt acgcataatg
     9721 ggttgcggca tccccgcata aaccggaaca ttaatctcca gtttctggca aacattcagg
     9781 ccattaatta atgttttatc aagcgtctga ttacccacta caatagtgat gcctaataaa
     9841 tctattgccg gatgtttcgc tgccatcatc attgcaatag catcatcatg acccggatca
     9901 caatccagaa taatttttct cttttccatt gtttatttcc tctgtttcca gttgcgttat
     9961 ttttttctac agcaaagaaa attcgaccgg gccgatgatt gaatcttaac aacagcgtac
    10021 gtatgctaaa tatgagaaat ctcatagcgg ataaacatcg tgaaagaaat ccacaataat
    10081 gaccttaagc agcaattgat gagtgaatct gcgtttaagg attgcttttc aacggatgtt
    10141 tcagccgata cgcggctgtt tcatttttta gcgcgtgact acattgtgca ggaagggcaa
    10201 cagccgtcat ggctgtttta tctgacgcga ggccgcgcca ggctttacgc cacgcttgct
    10261 aatggtcgcg tgtcgctgat cgatttcttt gccgctccct gttttattgg cgagattgag
    10321 ttaatcgaca aagaccatga accgcgtgcg gtgcaggcta ttgaagagtg ttggtgcctt
    10381 gcgctcccta tgaaacatta ccgtccgctg ttattaaacg acaccctatt tttacgaaaa
    10441 ctttgcgtca ccttaagtca taaaaattat cgtaatattg tttctttaac tcagaatcaa
    10501 tcatttccgt tagtgaatcg cctggcagca tttatattac tctcacaaga aggtgatctt
    10561 tatcacgaaa agcatacgca agcggcagag tatttaggcg tttcttatcg acatctttta
    10621 tatgttctcg cgcaattcat tcacgacggt ttattaatta aaagcaagaa aggatatcta
    10681 attaaaaaca gaaagcattt gtcaggactg gcgctggaga tggacccgga gaataaattc
    10741 tccgggatga tgcagtaaaa attatttgca atagcgcgat tgccggatgc aacgcttaat
    10801 acgttttatc cggtctacaa atcgagcatt acgccagacc aataaagaac ccggcaatag
    10861 tcgcactcat caggttggaa agcgttgctg ctgccagcgc ccgtaaacca agctgggcga
    10921 tttctggagc acgttttggc aaaatagccg aaaatgcgcc aacgacaaca ccgatagaac
    10981 caaagttagc aaaaccacaa agcgcaaagg atataatcgc cacggttttc acttccagcg
    11041 tgccgcccgt ttgcaggtat ggggagaaac tcaggtaagc gacgaattcg ttaatcgcca
    11101 gtttctgccc aatcaggcta ccagcaaggt tggcatcact ccagtccaca cccatgatcc
    11161 acgccagcgg tgccagcaca tagccaaaaa tactttccag agaggcattg gcgaaaccaa
    11221 accagccgcc aattccgccg ataatgccgt tgatcagcgc aataattgcg acaaacgcca
    11281 ttaccaccgt cgctacacca gcggcgattt tcagcccggt catcgcaccg ctcgccgccg
    11341 cttcgataaa gctttttggc ggcgtttcgc tgaacgacag attttcaaat gtgacctgcg
    11401 aaggctcggt tgccgggcta agaatacgtg caaacaaaat accgccgggg atcgccatca
    11461 gcgatgccgc taacaggtag tcaattggta cgcccattcc ggcataacca atcatcatcg
    11521 aaccagcaat ggacgccatc ccgctacaaa ttgcggtaaa caactcgttg cgattcatgc
    11581 gatcgataaa cggtttaacg atcgccggga tctcattttg cccgaggaaa atagtggtaa
    11641 ccgcaacaaa agattcgatt ttgctgatgt tgagggcttt ctggaaaatg ctgccaagga
    11701 tgcgaatcag cagccccatc acgccaatgt agtacagcag actgatgagc gcagtaacga
    11761 aaataatcgc cggaagtacg cgaaaggcga agataaaacc cgcaccgtca aacaggacat
    11821 ccattttcgg cccaaccagc gaaccaaaaa tgaaggcgct accggcatca ctgtaagaca
    11881 tcactttatg aacgcctaat gccgcctgtt ctactgccca ttttcccggt gggaagtaga
    11941 gcatgatgcc accaatagcg atttgcagca gcagtgcggc tccaaccgtg cgcaaactga
    12001 tgctcttttt attcactgac aacagaaatg ctattgccag taacaccacc atccccacaa
    12061 cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg cgtcagcgcc
    12121 aggttttggt atgcttgatg agtacgggcg acggctttct gcccgtcaga aattaacccg
    12181 cgagacgctg gtattctttg gcaatttcac tcgccagaat ggcgttgttg aacaccagct
    12241 ggatattgga tttcaggctg tcaccgccgg tcagctcagc aacgcgcgcc agcaggaacg
    12301 gtgtactttc tttaccaata acgccctgct cttcggcttc ggcgacggct tgatctatgg
    12361 ctgcattgat agattcctcc ggcatcgcaa actgttccgg gatcgggttc gctaccacga
    12421 ggccaccgtt cagcccgctt tgccatttca ccgccattgc acgggcaatt tcactggcgc
    12481 tgtcgagacg aatgctgacg tcaaacgagc tggtacggca gaaaaacgca ggcagcgctt
    12541 tagtctgata gccaattaac ggcacaccga aggtttctaa atactcagtg gttaatccta
    12601 aatcgagaat agatttcgcc ccggcacaaa caacggtgac attagtattt gccagttctt
    12661 gcaaatcggc agaaatatcg aaggtatgtt ccgccccgcg atgcacacca ccaattcccc
    12721 cggtggcaaa tactttaatt ccggcaagcg ccgcaataat catcgttgaa gccacagtgg
    12781 ttgcgccatt ttttccggcg gcaacaacaa aaggtaaatc gcgacgacta actttggtca
    12841 cgttatgccc ttcacgaccc agtaattcaa ttttttcttt gcttaaaccc actttcatca
    12901 cgccgccaat aatggcgata gtggcaggta ctgcgccctg tttacgaata gtttcttcta
    12961 cttcaattgc ggtctgggca ttttgtggga acggcatccc gtgagaaata atggtcgatt
    13021 ccagcgccac aaccggtttt ttgtttttta aagcgtcctg cacttccggg gaaatttgta
    13081 ataattcagg ggaaattttt aattcagaca ttctgcgttc tccactaacg atataacgtt
    13141 ggcaatcgat aaatcggggt tattggtgta ttcacaggag agcgccattg acgaacatcc
    13201 ctgtgcgaaa cgaacagatt cggcaaacgg cattccgtct acccaacacg aagcaagtcc
    13261 cgccatcatg gcatcgcccg ctccggtaac attaataaca ttggttttga tcggcgcaga
    13321 ccagccattt ttaccgctga tatcgctgta ataaacgccg tcgccgccca tgctcaatac
    13381 cagtcggttc aggccatgtt gatggaacca ggcagcaact tttgccacat cgtcacgccc
    13441 tgacagcgca atcccactca gagtttccgc ttcaaggcgg tttggcttca gagtgtggat
    13501 ctgatttagg cggtcgcgca ctttgacaca tttccacgct gaaaccggat cgacaaatac
    13561 gggtacgttg gcggcattat ccagaatcca tgccagtgcc tcttcactga tattacagtc
    13621 cgcgacaatg acctttgccc tctgaataaa ttcacggtgc tgtgcgagat attcagctgt
    13681 aatagcgttg ctaatattca tgtcatttat agcaaccagc atttcaccgg tattatcgag
    13741 taatgataaa taactcgacg tattttctcc cggcacaatc aggcatttat cgacataaac
    13801 accagattga ttggtttgcg ttagcagcga ttgaccataa aaatcactgc ctacggcgct
    13861 cagtagccag gctttgttac ccagcaacgc caggttttgt gcaatattgc gccctactcc
    13921 accaggcgta aattttattt tacctggatt tgaatccgca taatttaatg attcatgtga
    13981 atatccggcg acatcaatat tcgccgaacc tataattacg acataatcct tttcgcgcat
    14041 agcacgtcct ctggtaaaaa attaccaacc ctgtcaatcc acaagaatat agatactgca
    14101 aagacggttt aaacatgtgt tcatatttaa acatgtgctc atagtatgca tgttttacgt
    14161 aaagtaaatt accgccagag aggcaaaatg ggcagatttg agcaggctca caaaatgcga
    14221 ttaaatgcct gaattacaaa agcgaggaat ttgtgacggg atgcacagaa caggaacact
    14281 aaaaaagggc agaaaataat ctgcccttga ggaaataacc ttagtgtttg atgaacgttt
    14341 gaagtttcta ctgaaagata aaaccattta aattcataaa attacattca caagctggtc
    14401 attctctttg cgctcatcaa atgttacagg gcaggaaatt tctgccctgt aacacacctt
    14461 ttattacgct gctttcgcta ctgcgtccac ttccggacgt ttcaggaaag cataggccaa
    14521 acccgccacc agcgtaccgg caataattgc taccaggtaa cccaataccg gcgtaatagc
    14581 gccagggatc agcagaacaa acagaccacc gtgcggtgcc atcagtttcg caccaatcgc
    14641 cattgagatt gcgccagtca gcgccccacc cacgatacag cacggcagca cacgcatcgg
    14701 atcacgagca gcaaacggaa ttgcaccttc cgaaatgaag cacagtccca ataccagagc
    14761 ggctttgcca ccttcctgct gcgctttgtc gaatttgcga cgcgccacca ttgttgccag
    14821 gcccattgcc agcggtggca ccatacctgc cgccataatc gccgccatcg ggccataggt
    14881 ttgagtactc agcagaccca caccgaatgc gtacgctgct ttgtttaccg gaccgcccat
    14941 gtcagcacac atcatgccac cgaggatcgc ccccagcaga accgcattcg cagtccccat
    15001 ggtctgcagc cagtgagtca gcccttcgag aatgccagca actggtttac cgatcaggta
    15061 gatcatcgcc agaccgacca ccagactgga aattagcgga atgatcagga tcggtttcag
    15121 cgcctccata ctctgcggca gcttcagttg cgtactgatt aacttcgcaa tgtaaccagc
    15181 caggaagccc gcaataatac cgccaatgaa gccagaaccg gtgctgaccg ccagcatacc
    15241 gccaatcaga cccggcgtga ggcccggacg atcggcaatg gagaaggcaa tataacctgc
    15301 cagtaccggc accatcagcg caaaggctga accaccacca atctgcatca gcgccgctgc
    15361 caacgtaccc ggctctttaa acgcttcgat accaaaagca aaagaaagcg cgatacacag
    15421 accaccagca acgaccatcg gcagcatgta agagacgccc gttagcaagt gacgataagc
    15481 gcctgcactc tctttcttac cttcagtggt cgccgtttga gctttgcccg ccggttcata
    15541 cggcgttgct tcagcaaccg ctttatccag ttcctgcgcg gttttcttca gcgccagacc
    15601 ggtagaggta cgatacatcg gtttaccagc aaatttcgcc agatccactt cgatatctgc
    15661 cgccacaatc accagatccg ctgcggctac ttcctcggga gtgattgcat tacccgcgcc
    15721 aacagaacca cgggtttcaa ctttcaccca ccagccacgt tttttcgctt cggtttcaat
    15781 ggcttcagcc gccataaagg tgtgtgctac gccagtcggg caagcagtca ccgcaactac
    15841 gcgtttcgga ccgctggcgg caaccggtgc tgtcgcagtg accggcgcag tgtaaggttt
    15901 cgcatggcct ttggcttcac tcaggaacag ctcagggtgc gcaactgccc gggaaatatc
    15961 acccagccag acatttttac cgttcagcgc gctgtcattc gggatggaat caccgagaac
    16021 aatcgccatt tctgcgtcgt tcggattgtc gatgatttcc agttttgctt ttcgcgccgc
    16081 cgcgcccagc agggtcttcg ccatataggc gcgtgcctga ccgagattag cgtcaataat
    16141 cagcagcgtt ttcattatgc ctctcctgct gtcagttaaa aggttgtaag tcgacgcgcg
    16201 ccatcattgc ggccaactgc ggacgatcgg taatacccac attgctttga cttaccgcca
    16261 gggctgcaac agctgtcgcc agacgcagtg tgtgttcact ggattcacgc atcagcaagc
    16321 cataaatcag gccaccaacc atagaatccc ctgcgccaac ggtgcttacg acatcgactg
    16381 acggtggttt agcgatccat tcgccggagg cattaaccca aagcgcgcct tcggcaccca
    16441 gtgaaataac aacatgcgca ataccttgtt cacgtagcgc atgtgcagct tcaatcacat
    16501 ctttcatttc aggcagttta cggcctgccc agatttccaa ctcgcggcgg ttaggtttca
    16561 ccagccacgg tgccgctttc aaacctgcta ctaacgcttc acggctacta tcaaagataa
    16621 tgcaaggaca ctgactacgc aggcgagtca tccagtcggt gaacgcttcc gggctgacgc
    16681 ctgacggtaa gcttccgctg acacagacca tatcgaactg accgagccag ctcagagaat
    16741 cagtcacaaa gcgttcccag tcggcggggg tgacttcaaa acccgagaag ttgaagtcgg
    16801 tcacttcgcc gtctttttcc gtcagcttaa cgttaattcg ggtgcgcccc tgtacaacct
    16861 ggaaacggtt ggcaatgccc agctcgctga acagttgctg aaaaccatcc tgattgtctt
    16921 tacccaggaa gccgccaacg gtgacatcaa ttcccaggtc ttttaatact ttggccacgt
    16981 tgatgccttt acccgccgca tgcagaccgg tggttttcac caggttcact tcgccgcgtt
    17041 caatttccgg gcagaaacca acaaggtcat aagccggatt aagggtgatc gtagcaacac
    17101 gtctgctcat tatgcgccct ccccaagacc agcagcgata gcgtcgccaa tggctttcag
    17161 cgcctgttca gcatctgcac cctgggcggt aaagcgtagg cgatgacctt tcttaacgcc
    17221 aagtgccaca actttcatca gactacgtcc gtttgccggt ttgccggtgc catcaaggtt
    17281 tgtcacggta atatcactgt taaattgttt aatggtattg accagcatgg tacctggacg
    17341 agcatgcagg ccgtgttcat tgcgcaccac aaactccgcg cttaacacgt cgtcggtcgg
    17401 cgcatcatcg ctggtcagca gcgccagcaa cgttgccgca tccgctttca gcaagcggtc
    17461 agctttattg tcgagcaaca aatcagcgag acgcttaaga accgcgatgg gctgatcgtc
    17521 attcatcgcc acactcacca gcatggctgc cgtttcgccg tccacatcaa aagcatttgc
    17581 cgcacggctt accgcaatcg cactacgcag attgccttcg gcgctatcgc tcagccagat
    17641 accctgtccg agattcagcg gttgttcatt gatggctttg gtgacgaaag tggcgtcaac
    17701 tgccctcgcc tctttcagac gcgcagcgtt cagcgcctga agagtcagca gatcgctggc
    17761 gacgatatcc agcgtcagca tttcgttgtc gagcttcagc tgctcactct gcttttcgcc
    17821 catcagtaat gcgcgaagtt cttctgctgt tgttgctgac ttcagttgtt cagcaacgga
    17881 atcatcgctc agtacgtggg tcagctggcg tagcaggccc agatgtttat ccgagctggc
    17941 agcaataccg attgccacgt acgctacctg accgtcaccc caggtgacgc cttccgggaa
    18001 ctgaaatacc tgaacgccgg ttttcagcac ctgatcgcgg gtgtcggtag tgccgtgtgg
    18061 aatagcaata ccattgccga ggaacgttga ggtttgctgt tcgcgcgcca gcatgccatt
    18121 gacgtagcct tctgctacat taccggcctg caccagcgcc gcagcgacct ggcgaatcgc
    18181 ctcttctttg tctccggcct tttcgcccgg atggatgtcc tgtacggata actggaacat
    18241 agttctcctc tcttgctgaa ttgaaacgat tcagcctcta tgagaaaaaa agcgccaacc
    18301 tggcttaggg ttaaagacaa gatcgcgctg aaacgtttca agaaagcata atacttctgt
    18361 ttcagcacgc aaggaaagcc gtaaagttat gagcaaaagt ttgatgtgct gcacaatttt
    18421 ttgtcatttt tcctaattgc tgatgggaaa atccgttatc agcaatttca tttcagcagc
    18481 gtcagcagcc aatcagcaac agtgaaatgg cattttgatt attcgtggcg cttttgattt
    18541 cgctgtctgt ttattttctg acaagcagcg taaactccgc gtcttcctct tccagtgatc
    18601 gaccagcatg cataactccc ccgcagtctc cagcgcgaaa tcgtttgacc tgacctcgac
    18661 ggcgttttta atcgttgcct ttctcaccgg tattgcgggt gctctgcaaa ccccgacact
    18721 cagtattttc cttaccgatg aagtacatgc ccgtccggcg atggtgggat tcttctttac
    18781 cggcagcgct gtcattggga ttctggtaag tcagtttctc gctgggcgct ctgataagcg
    18841 cggcgatcgc aaatcgctga ttgtcttttg ctgcctgtta ggcgtgctgg cctgcaccct
    18901 ttttgcctgg aatcgcaact actttgtttt gctattcgtt ggcgtctttc ttagcagctt
    18961 tggctcgacc gctaacccgc aaatgtttgc ccttgccctt gcccttgccc ttgcccttgc
    19021 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
    19081 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
    19141 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
    19201 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    19261 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattgat
    19321 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    19381 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    19441 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    19501 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgccattct
    19561 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    19621 ctattttcag gatttgatgc ccggtcaggc aggttcagcc accacgctct ataccaacac
    19681 gtcgcgcgtg ggctggatca tcgcaggatc agtggcgggc atcgtcgccg agatctggaa
    19741 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    19801 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    19861 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    19921 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    19981 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    20041 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    20101 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgtgaata ccacttgccc
    20161 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    20221 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    20281 gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    20341 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    20401 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    20461 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    20521 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    20581 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    20641 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    20701 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    20761 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    20821 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    20881 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    20941 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    21001 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    21061 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    21121 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    21181 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    21241 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    21301 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    21361 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    21421 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    21481 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    21541 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggccatgt
    21601 ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg ccgggtggat
    21661 aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    21721 cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg cgattagctg
    21781 cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc ctgcctagga
    21841 agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcgtat
    21901 gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat tcgcccatat
    21961 cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa tgctggatga
    22021 gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat
    22081 tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga tggatggcag
    22141 ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc gtgaaacgga
    22201 ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacatcagcg gcgttgatga
    22261 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    22321 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    22381 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    22441 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    22501 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct tttttagcca
    22561 agattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    22621 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    22681 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    22741 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    22801 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    22861 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    22921 cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc tcgatccacg
    22981 cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    23041 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    23101 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    23161 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    23221 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    23281 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    23341 cgctgataca gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    23401 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    23461 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    23521 cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc gtttagcgac
    23581 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    23641 ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc ctggtatatt
    23701 cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag
    23761 aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgaccaacaa tcgcgccttc
    23821 gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct gaaagaaaac
    23881 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    23941 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    24001 accgatatta accgcgtcag cgaactgctc tctgtcccca cgaaagatgc ctcacgagat
    24061 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    24121 ttcggcaaag ttgcaggcct tatcgccctt actatttttg tggtttttgc atttcccaga
    24181 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    24241 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    24301 aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    24361 atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatctatc
    24421 gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaattgtac
    24481 tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    24541 taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg
    24601 tagattatcc tcacagcata taattttgtg cgttagtcca caaatttggc cttaaggaat
    24661 tgtttcaaca tgcccaggta attagcctcg tgtcgcttgg cattttttta taacgatatt
    24721 tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga
    24781 tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc atgtagtgca
    24841 aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca
    24901 ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga
    24961 attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa
    25021 aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt tggcttagaa
    25081 cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtctcccg cagtaatttg
    25141 cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt cggtatttat
    25201 atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg
    25261 aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa
    25321 taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag
    25381 gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc
    25441 tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc
    25501 atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgtagcaggc
    25561 gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca taagcacgaa
    25621 gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac
    25681 ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac
    25741 cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa
    25801 gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg
    25861 attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg
    25921 gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt
    25981 gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa
    26041 gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt
    26101 ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt
    26161 cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt
    26221 ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct gatgagcgta
    26281 cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg
    26341 tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt actgctacgc
    26401 tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg
    26461 caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca
    26521 gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc gccggaccat
    26581 ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct gcccgcacac
    26641 catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca
    26701 caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc
    26761 gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt
    26821 ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg
    26881 acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac gccggaacaa
    26941 gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg
    27001 ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca gtggtaaggt
    27061 gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta
    27121 aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc
    27181 ttcggtgcgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc
    27241 cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg
    27301 cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc actgcgcggc
    27361 aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc cgatgacgaa
    27421 ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga ttattcctgg
    27481 gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat tactgcccgc
    27541 gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt tcgtctggtc
    27601 tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa
    27661 cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg
    27721 aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccac
    27781 gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca
    27841 gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta cgattattac
    27901 ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa
    27961 aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc
    28021 atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc
    28081 caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc ctttgacttt
    28141 gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag ttattttcaa
    28201 aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca
    28261 atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatctaaa
    28321 ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc
    28381 tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct cagctttgaa
    28441 ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa
    28501 cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg
    28561 cgcagtcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg gcccagttcc
    28621 gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg
    28681 caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa
    28741 aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg
    28801 ccaatgtggt acatggcgga agactgtctc gcctggtggg ataaattctc ccaacccgct
    28861 gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa taaagcggcc
    28921 aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat tcgccgtctg
    28981 ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat tgtgcaaatt
    29041 gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttgataa tgccggagta
    29101 ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat
    29161 atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat cgctgagatc
    29221 actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat gctctgggac
    29281 tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct gacgctgatt
    29341 aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg
    29401 gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt tgacgtctgg
    29461 agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg
    29521 attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc
    29581 gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg
    29641 ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct gaccaaaaac
    29701 tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt
    29761 gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt gattgccggt
    29821 tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt
    29881 tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta
    29941 atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat
    30001 atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg tcgcgactca
    30061 gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc tactggtcgt
    30121 tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc gctaacgata
    30181 aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg
    30241 aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac
    30301 aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta
    30361 tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa
    30421 cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg atctcggttc
    30481 tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac
    30541 aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa gtatggtcgg
    30601 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
    30661 ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
    30721 agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt ggcgtcagcg
    30781 atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt
    30841 taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc ctcggcttcg
    30901 gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc
    30961 aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga
    31021 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
    31081 caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca aaccgtacgt
    31141 acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt
    31201 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
    31261 ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct tcatgccagc
    31321 gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca ggaaccgatg
    31381 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
    31441 cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga tcgcgtcggt
    31501 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
    31561 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
    31621 ccgaccaccg cactggacgt ctctgtccaa gcgcagattt tacagctgtt gcgcgaactg
    31681 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
    31741 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
    31801 acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag tgaaccgtca
    31861 ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag
    31921 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
    31981 aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg cgagtctggt
    32041 tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca gggcagcatc
    32101 gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc tactcgtcat
    32161 cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
    32221 cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
    32281 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
    32341 ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt
    32401 aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg
    32461 caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
    32521 agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
    32581 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca ggagtatacg
    32641 cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc
    32701 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
    32761 aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa
    32821 cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt
    32881 aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
    32941 acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga ttgagagttg
    33001 taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt tacatcaatt
    33061 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg
    33121 tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat
    33181 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
    33241 taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg tggttgacat
    33301 gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg ttttttcggc
    33361 cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat tgaccaaatc
    33421 atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg
    33481 ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
    33541 agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg
    33601 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc
    33661 ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcggacg
    33721 aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc aaagtaataa
    33781 ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc ggcgcttaag
    33841 aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag gacacgttta
    33901 tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg ctgacgacgc
    33961 tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag aatcgccgct
    34021 aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca gccgccaact
    34081 atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat catccgcgag
    34141 aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct ggccgcagcc
    34201 gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg tgccaacgca
    34261 cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg cttacgcccg
    34321 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
    34381 ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat taccggtagc
    34441 gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    34501 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcattgaaa
    34561 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    34621 tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac gtggttaccc
    34681 acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    34741 tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc gttatgcagc
    34801 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc cagtgtcacc
    34861 agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca
    34921 tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc gtgcagtttc
    34981 cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc gtccgtggag
    35041 caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg ctgcgccagc
    35101 gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc attacgaacg
    35161 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    35221 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    35281 aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta tagcggtcta
    35341 ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa tctacctacg
    35401 cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg ccacgctcaa
    35461 ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg caggtaaaag
    35521 cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc tggcacacgt
    35581 taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg aagccgatat
    35641 ttttgccgcc gggctaaagc gcaaagagaa ccacggtaaa gtggtatttg gcagcgttca
    35701 gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga ttgtcgatga
    35761 atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca ctcacctgac
    35821 taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc gattgggcaa
    35881 aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga aagccctttt
    35941 ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct atctgacgcc
    36001 gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc aggcacagag
    36061 taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc aacgcattac
    36121 gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg taatgatttt
    36181 cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg aagatgctgc
    36241 actgattact ggcgacaccc ccggcgctga gcgcgatgtg ttaattgaag attttaaagc
    36301 ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg acgccccgca
    36361 cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc aacaaattgt
    36421 cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg attatgcggg
    36481 taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa gtgacaacgt
    36541 tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg ggaaaacgac
    36601 cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt ttgaagatga
    36661 cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc cgcaatgtaa
    36721 cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac tggttgatcc
    36781 ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac gctgtagcgg
    36841 catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca cctattacga
    36901 tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc agcgtaccgc
    36961 cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc tgcgctggat
    37021 caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt ttgtcgtcgc
    37081 ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg aaggtcgttt
    37141 tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata aggcgattga
    37201 gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacttgt tgctgggtcg
    37261 cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca gaagtacgta
    37321 aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc ccggcaatca
    37381 tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa gtcatgaaca
    37441 tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac ggtaaagaaa
    37501 tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg cagcacatcg
    37561 acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg cggggttttt
    37621 tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca gataaggcgt
    37681 tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga cgtccggcgc
    37741 tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc ggttgctgga
    37801 tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa gccaccgccg
    37861 ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa gctctcttcc
    37921 agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac gcctgccagc
    37981 tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc attacagtag
    38041 ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc ttcggcagtg
    38101 aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa tccttcgctg
    38161 gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc tttcaggaaa
    38221 gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat acgcgcaaca
    38281 atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt ttcgttaacg
    38341 cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc cagataacgg
    38401 tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa ttcgtcacgc
    38461 aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg acgctgtaag
    38521 cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt atttttggcg
    38581 ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg ttccagcaat
    38641 gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg gtgcagagca
    38701 atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg tattcaacca
    38761 ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca gcgtaatgag
    38821 aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata tgttgccctt
    38881 tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa tgaacaggtt
    38941 gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc gaccgacctt
    39001 tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat tgccccggcc
    39061 caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat caacgaagac
    39121 aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc gctaccgtga
    39181 aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata ttctgctttc
    39241 gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc ttgctggtcg
    39301 catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg ccacctactt
    39361 gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga ggtttttgtc
    39421 cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag tctttactcg
    39481 tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag acgaaaatga
    39541 gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat tgcttgaact
    39601 ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc gacgcttcgc
    39661 gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg tgtatatctg
    39721 ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc cgctttcgta
    39781 cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc aggagtatca
    39841 acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc cgttaagcga
    39901 actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg tcgatggcct
    39961 gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg agcaaaatat
    40021 ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct ttggcctgtt
    40081 ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc ctgcggcatt
    40141 aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag atggctttac
    40201 cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg tgcgcaccca
    40261 atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg cacaagaaga
    40321 taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca gcaatcagca
    40381 ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga tcaaccgcgt
    40441 gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta tcactgccgg
    40501 tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg gtcatctgca
    40561 ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg ctctgactga
    40621 tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca cacctgccag
    40681 cgaatattca caaggtcagg atttgttcaa ccctcaacgc cgtcattact gggttactgc
    40741 tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc tgaacaataa
    40801 cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa aaccacagtt
    40861 aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact gattaattat
    40921 aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc
    40981 acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg
    41041 ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta cggttggttt
    41101 ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct gtttattttc
    41161 tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc tacccctgcg
    41221 ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc
    41281 ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc atcgtcatta
    41341 actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag ccagggttcc
    41401 agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt
    41461 aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta gtcctgacca
    41521 tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc gtcaacccaa
    41581 cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt
    41641 gccccattac tcatattgcc atggatggtg ttggcaaaac ggtcaacttt caccacccca
    41701 tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt atagctatcg
    41761 atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc aaagaacaag
    41821 ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc
    41881 gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt
    41941 gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag acgctcacgc
    42001 acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt cagcacatca
    42061 gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg gttgccatta
    42121 tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgta
    42181 aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc tgccgggctg
    42241 gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt gacgttgagc
    42301 tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt tcccgacagg
    42361 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    42421 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    42481 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    42541 acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    42601 actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat gacctcaaga
    42661 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    42721 ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat atttccattc
    42781 tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc agtatccttt
    42841 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg cgtgtcctcc
    42901 ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc atcctttata
    42961 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    43021 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
    43081 ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg caaacttccg
    43141 ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg accgagacgg
    43201 tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc tgctttctga
    43261 tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt ttcgatagcg
    43321 gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat cgtggtatca
    43381 ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt tgccgcgcca
    43441 cccatttgaa caatttgata accatcattt aatactgagt attctgcggt accattcatt
    43501 tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa ttcttttata
    43561 tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac cagctggata
    43621 tctttttcca ggataacacc atcaactgtc tcatcatact caactgttga tgccaatgct
    43681 atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca aagagaaaca
    43741 gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta aaagcattgg
    43801 gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg tttaaagaaa
    43861 tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct gatgcaaaat
    43921 tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt ttcactacat
    43981 gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac gctacgctca
    44041 aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac gccttttcag
    44101 gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt actggagctt
    44161 gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat cgatctggcg
    44221 aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat gagtggcctg
    44281 gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat cctgaccgta
    44341 tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg ttatctgttg
    44401 aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa aggcagcaaa
    44461 gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg cgcggaagaa
    44521 gatcccttca gcgtgctgac ggagcgcgag ctggatattc tgcacgagct ggcacagggg
    44581 ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt aaaagtacat
    44641 attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac cattctgttc
    44701 ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg gctgagattt
    44761 atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg atcgatcaat
    44821 ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa
    44881 ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct ctttttattt
    44941 actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa tcaccgcacg
    45001 gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa cggcttcgcc
    45061 aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga tattgctatg
    45121 gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt tgggatcaga
    45181 tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg gatcgaggcg
    45241 atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg
    45301 gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag tacgcatccc
    45361 cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt ccagcgccct
    45421 gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt
    45481 gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac ccggcagata
    45541 aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg cttccggcac
    45601 cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc caatagccac
    45661 tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa
    45721 gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac cttcctgcat
    45781 cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat cggcaatgct
    45841 gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg
    45901 tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccga agatcatcag
    45961 catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt ctcccacagc
    46021 ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg atagcgaatg
    46081 ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag atccagccct
    46141 aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc tttcagccag
    46201 ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat gccctgcgct
    46261 gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt cgcccagacg
    46321 ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg
    46381 tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga ttccagattg
    46441 gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc ggcaatcgcc
    46501 aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc ggcgcagttt
    46561 tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt ccgcccaacg
    46621 ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa agacgcacgg
    46681 cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc gtgatgccgc
    46741 cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttccgcat
    46801 acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca ccgccgcgcc
    46861 agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca tgaatatcga
    46921 cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg ctaaaggcaa
    46981 tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa taactgaagg
    47041 tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc accgcaatcc
    47101 agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg
    47161 gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa ttgcggattt
    47221 tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt agcgcaaacg
    47281 gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca
    47341 gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc agcatcgcgg
    47401 cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta
    47461 cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca
    47521 gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg
    47581 ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag aaggcactga
    47641 ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc gccagtgagt
    47701 gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga
    47761 accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc acaatgccga
    47821 gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca taagtgctgt
    47881 ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag aaacccacgt
    47941 accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac agcgggttaa
    48001 gatcgcgccc ttcaatcgtg aagttcggca gcgtgcgtga gaacgggtta gaggtaaaga
    48061 gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca cgggccacaa
    48121 tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg ctcatcagca
    48181 gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg cgataccaca
    48241 ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg ttgaccacga
    48301 aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc caggcaaaca
    48361 agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc ggatacacgg
    48421 acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt tccggcatca
    48481 tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc
    48541 aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg cgaggatatg
    48601 attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg
    48661 caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg tgaaggtcac
    48721 tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc caacgcgcag
    48781 acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa tttcccccgg
    48841 cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc
    48901 cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca
    48961 gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg cgccacacca
    49021 cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag cggaataacg
    49081 gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc
    49141 caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg
    49201 gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc agcgtggcag
    49261 acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc ggatcgatac
    49321 tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg gagtaatgaa
    49381 taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg cccgccagac
    49441 ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac agcagcacca
    49501 gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt tttccccatg
    49561 cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca
    49621 ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc accgccattg
    49681 atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc
    49741 tatttccctg ctgataatca gccggagcca agccgaatcc ccagatccag ccgacggtaa
    49801 gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc
    49861 gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca
    49921 aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg
    49981 acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc catcaacggg
    50041 caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag
    50101 cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac
    50161 gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca gcgccatcac
    50221 ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa ggatgagtaa
    50281 cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg
    50341 taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg
    50401 ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg gtgcaatacg
    50461 cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta caatcaggaa
    50521 gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca gttcaagacg
    50581 gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg gcgaatttta
    50641 ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc ctgctccgta
    50701 tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc ggtaaaaggc
    50761 tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc gacgcggcgt
    50821 tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc
    50881 gccagggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg cagattttct
    50941 aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg
    51001 tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc agcgtcaggg
    51061 cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg
    51121 atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa ggttcgttca
    51181 tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc ctgttaaaaa
    51241 cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg gcaatcaata
    51301 caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg
    51361 gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt attgtccttc
    51421 atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt ctgcggcgtg
    51481 tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc
    51541 ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg
    51601 ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat ttcgtggcag
    51661 ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc cccccagaag
    51721 acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg gctgggggta
    51781 cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata
    51841 acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat tggcgcggta
    51901 tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc
    51961 actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc agtggtgcga
    52021 tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac ctgataacct
    52081 tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc
    52141 tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa
    52201 tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat
    52261 tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg
    52321 tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat
    52381 cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg
    52441 gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga tcgctggccg
    52501 caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc ggacaaatgt
    52561 gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca
    52621 gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg gggttgatcc
    52681 attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc aacagcacgt
    52741 agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg cgtaaccagt
    52801 tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcccg
    52861 ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg gcaggcagat
    52921 gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg gtgtcgaata
    52981 acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga ccgctcaaaa
    53041 acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg tgactgcgcc
    53101 accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc
    53161 ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag tgacagcggt
    53221 aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc gcaaccggta
    53281 caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg cgtgttgcgc
    53341 tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag
    53401 cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag ccccatccgc
    53461 gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac tttggcgcac
    53521 ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc
    53581 gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac
    53641 tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc
    53701 aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca
    53761 gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt
    53821 ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag
    53881 tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac cagctgtgcg
    53941 gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc gcgcgtttca
    54001 acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga
    54061 tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa ggcgcggtgc
    54121 agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac gcgtccggta
    54181 gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa cggcagtgcg
    54241 aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc cgctttcacg
    54301 tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac caccggccag
    54361 cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc gtgaccgcga
    54421 ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag ctcgcgggat
    54481 tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac ttcgggggtg
    54541 gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc cagcagctct
    54601 tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg ccagagatcc
    54661 gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg gcgttcggcg
    54721 ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa gtcggcggcc
    54781 agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg atcgcgccag
    54841 cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt acacatggtc
    54901 cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc atgaagaccg
    54961 attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat atcacgatgt
    55021 ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc
    55081 gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc cggctgggaa
    55141 attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac accacgggta
    55201 tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc
    55261 tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt tttttccagc
    55321 gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa
    55381 cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc
    55441 gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat cgcattgttt
    55501 tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg cgtaaagatg
    55561 atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac cgccacggtg
    55621 acgttctggt tagagagacg acggttagta atgcgtgacc agaggatcgg gtgcatctcc
    55681 gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc
    55741 atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc catacagtga
    55801 cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt ggacgcggca
    55861 taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt
    55921 tctttcaggg cggttttgaa tttctcttcc atcacatcga aagcctgatc ccaggtgatt
    55981 ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag cggctgcgtc
    56041 aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca gttcaggcca
    56101 cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg ctgcgttccg
    56161 accagaacgc cgcaaccagt accgcagaaa cggcacggcg
//
LOCUS       DADLSE010000009        56007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLSE010000009 DADLSE010000000
VERSION     DADLSE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.009"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..350
                     /gene="cmtB"
                     /locus_tag="JN692_000553"
     CDS             <1..350
                     /gene="cmtB"
                     /locus_tag="JN692_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HAY7262950.1"
                     /translation="LDKNYISENYIQAIKDSTINNGPYYILAPGVAMPHARPECGALK
                     TGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSELLCEEEILEQLLTA
                     SSEKQLADIISRG"
     gene            378..1766
                     /locus_tag="JN692_000554"
     CDS             378..1766
                     /locus_tag="JN692_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HAY7262951.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITCLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLPVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1781..3058
                     /locus_tag="JN692_000555"
     CDS             1781..3058
                     /locus_tag="JN692_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7262952.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3055..4020
                     /gene="yggF"
                     /locus_tag="JN692_000556"
     CDS             3055..4020
                     /gene="yggF"
                     /locus_tag="JN692_000556"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7262953.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQMANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4042..4551
                     /gene="fumE"
                     /locus_tag="JN692_000557"
     CDS             4042..4551
                     /gene="fumE"
                     /locus_tag="JN692_000557"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7262954.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4548..5261
                     /locus_tag="JN692_000558"
     CDS             4548..5261
                     /locus_tag="JN692_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7262955.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5233..5910)
                     /locus_tag="JN692_000559"
     CDS             complement(5233..5910)
                     /locus_tag="JN692_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7262956.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPPAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(5904..6581)
                     /locus_tag="JN692_000560"
     CDS             complement(5904..6581)
                     /locus_tag="JN692_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7262957.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6569..6952)
                     /locus_tag="JN692_000561"
     CDS             complement(6569..6952)
                     /locus_tag="JN692_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAY7262958.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(6968..7276)
                     /locus_tag="JN692_000562"
     CDS             complement(6968..7276)
                     /locus_tag="JN692_000562"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262959.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWLHAITLWLLILAIVSTSQL
                     "
     gene            complement(7277..7855)
                     /locus_tag="JN692_000563"
     CDS             complement(7277..7855)
                     /locus_tag="JN692_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7262960.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7878..8309)
                     /locus_tag="JN692_000564"
     CDS             complement(7878..8309)
                     /locus_tag="JN692_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7262961.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8681..9700
                     /gene="epd"
                     /locus_tag="JN692_000565"
     CDS             8681..9700
                     /gene="epd"
                     /locus_tag="JN692_000565"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7262962.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9750..10913
                     /gene="pgk"
                     /locus_tag="JN692_000566"
     CDS             9750..10913
                     /gene="pgk"
                     /locus_tag="JN692_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7262963.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11128..12207
                     /gene="fbaA"
                     /locus_tag="JN692_000567"
     CDS             11128..12207
                     /gene="fbaA"
                     /locus_tag="JN692_000567"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7262964.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12398..13258
                     /locus_tag="JN692_000568"
     CDS             12398..13258
                     /locus_tag="JN692_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7262965.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13397..14032
                     /gene="argO"
                     /locus_tag="JN692_000569"
     CDS             13397..14032
                     /gene="argO"
                     /locus_tag="JN692_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7262966.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14125..14865
                     /locus_tag="JN692_000570"
     CDS             14125..14865
                     /locus_tag="JN692_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7262967.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15032..15928
                     /locus_tag="JN692_000571"
     CDS             15032..15928
                     /locus_tag="JN692_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7262968.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     LTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN692_000572"
     CDS             complement(15925..17403)
                     /gene="scpC"
                     /locus_tag="JN692_000572"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7262969.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYNNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN692_000573"
     CDS             complement(17427..18212)
                     /gene="scpB"
                     /locus_tag="JN692_000573"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7262970.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDNLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN692_000574"
     CDS             complement(18223..19218)
                     /gene="meaB"
                     /locus_tag="JN692_000574"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7262971.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMMLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDSDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN692_000575"
                     /pseudo
     CDS             complement(19211..21350)
                     /gene="scpA"
                     /locus_tag="JN692_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21554..22447)
                     /locus_tag="JN692_000576"
     CDS             complement(21554..22447)
                     /locus_tag="JN692_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7262972.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22830..23489
                     /gene="rpiA"
                     /locus_tag="JN692_000577"
     CDS             22830..23489
                     /gene="rpiA"
                     /locus_tag="JN692_000577"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7262973.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            23745..24977
                     /locus_tag="JN692_000578"
     CDS             23745..24977
                     /locus_tag="JN692_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7262974.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25206..25265
                     /gene="ibsC"
                     /locus_tag="JN692_000579"
     CDS             25206..25265
                     /gene="ibsC"
                     /locus_tag="JN692_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7262975.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25598..25657
                     /locus_tag="JN692_000580"
     CDS             25598..25657
                     /locus_tag="JN692_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7262976.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(25758..26360)
                     /locus_tag="JN692_000581"
     CDS             complement(25758..26360)
                     /locus_tag="JN692_000581"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7262977.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26381..26564)
                     /gene="ssrS"
                     /locus_tag="JN692_000582"
     ncRNA           complement(26381..26564)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN692_000582"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN692_000583"
     CDS             complement(26606..26935)
                     /gene="zapA"
                     /locus_tag="JN692_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7262978.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27103..27681
                     /gene="ygfB"
                     /locus_tag="JN692_000584"
     CDS             27103..27681
                     /gene="ygfB"
                     /locus_tag="JN692_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7262979.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27707..29032
                     /gene="pepP"
                     /locus_tag="JN692_000585"
     CDS             27707..29032
                     /gene="pepP"
                     /locus_tag="JN692_000585"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7262980.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQDEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29029..30207
                     /gene="ubiH"
                     /locus_tag="JN692_000586"
                     /gene_synonym="visB"
     CDS             29029..30207
                     /gene="ubiH"
                     /locus_tag="JN692_000586"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7262981.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLVAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30230..31432
                     /gene="ubiI"
                     /locus_tag="JN692_000587"
                     /gene_synonym="visC"
     CDS             30230..31432
                     /gene="ubiI"
                     /locus_tag="JN692_000587"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7262982.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGIQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31506..31589)
                     /locus_tag="JN692_000588"
     CDS             complement(31506..31589)
                     /locus_tag="JN692_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262983.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            31880..32974
                     /gene="gcvT"
                     /locus_tag="JN692_000589"
     CDS             31880..32974
                     /gene="gcvT"
                     /locus_tag="JN692_000589"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7262984.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            32998..33387
                     /gene="gcvH"
                     /locus_tag="JN692_000590"
     CDS             32998..33387
                     /gene="gcvH"
                     /locus_tag="JN692_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7262985.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33506..36379
                     /gene="gcvP"
                     /locus_tag="JN692_000591"
     CDS             33506..36379
                     /gene="gcvP"
                     /locus_tag="JN692_000591"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7262986.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDSHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            36646..37389
                     /locus_tag="JN692_000592"
     CDS             36646..37389
                     /locus_tag="JN692_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7262987.1"
                     /translation="MAIALVTGGSCGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37446..38879)
                     /locus_tag="JN692_000593"
     CDS             complement(37446..38879)
                     /locus_tag="JN692_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7262988.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            38924..39235
                     /locus_tag="JN692_000594"
     CDS             38924..39235
                     /locus_tag="JN692_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7262989.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            39399..40058
                     /locus_tag="JN692_000595"
     CDS             39399..40058
                     /locus_tag="JN692_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7262990.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN692_000596"
     CDS             complement(40254..41234)
                     /gene="ygfZ"
                     /locus_tag="JN692_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7262991.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTALAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41477..41743
                     /gene="sdhE"
                     /locus_tag="JN692_000597"
     CDS             41477..41743
                     /gene="sdhE"
                     /locus_tag="JN692_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7262992.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            41724..42131
                     /locus_tag="JN692_000598"
     CDS             41724..42131
                     /locus_tag="JN692_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7262993.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSCMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN692_000599"
     CDS             complement(42171..42692)
                     /gene="fldB"
                     /locus_tag="JN692_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7262994.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42804..43700
                     /gene="xerD"
                     /locus_tag="JN692_000600"
     CDS             42804..43700
                     /gene="xerD"
                     /locus_tag="JN692_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7262995.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDLPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43725..44435
                     /gene="dsbC"
                     /locus_tag="JN692_000601"
     CDS             43725..44435
                     /gene="dsbC"
                     /locus_tag="JN692_000601"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7262996.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            44441..46174
                     /gene="recJ"
                     /locus_tag="JN692_000602"
     CDS             44441..46174
                     /gene="recJ"
                     /locus_tag="JN692_000602"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7262997.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46265..47363
                     /gene="prfB"
                     /locus_tag="JN692_000603"
     CDS             join(46265..46339,46341..47363)
                     /gene="prfB"
                     /locus_tag="JN692_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7262998.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47373..48890
                     /gene="lysS"
                     /locus_tag="JN692_000604"
     CDS             47373..48890
                     /gene="lysS"
                     /locus_tag="JN692_000604"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7262999.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN692_000605"
     CDS             complement(48933..49481)
                     /gene="idi"
                     /locus_tag="JN692_000605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7263000.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            49536..49607
                     /locus_tag="JN692_000606"
     CDS             49536..49607
                     /locus_tag="JN692_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263001.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(49604..49729)
                     /locus_tag="JN692_000607"
     CDS             complement(49604..49729)
                     /locus_tag="JN692_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263002.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49731..51179)
                     /locus_tag="JN692_000608"
     CDS             complement(49731..51179)
                     /locus_tag="JN692_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7263003.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51615..53534
                     /gene="ygfT"
                     /locus_tag="JN692_000609"
     CDS             51615..53534
                     /gene="ygfT"
                     /locus_tag="JN692_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HAY7263004.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQRIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFERHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEQGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53534..54022
                     /locus_tag="JN692_000610"
     CDS             53534..54022
                     /locus_tag="JN692_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7263005.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDIISAPVMCHQCENAPCVGACPVQALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRLRQQRIVASGEN
                     PL"
     gene            complement(54058..55425)
                     /locus_tag="JN692_000611"
     CDS             complement(54058..55425)
                     /locus_tag="JN692_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7263006.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55461..>56007)
                     /locus_tag="JN692_000612"
     CDS             complement(55461..>56007)
                     /locus_tag="JN692_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAY7263007.1"
                     /translation="LDVYHQYGLTGKNCVFAHCVHLEEKEWDRLSETKSSIAFCPTSN
                     LYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFNMLQTLNEAYKVLQLQGYRLSAYEAF
                     YLATLGGAKSLGLDDLIGNFLPSKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMT
                     LGDDRSIYRTYVDGRLGYERN"
BASE COUNT        13567 a        13968 c        15079 g        13393 t
ORIGIN      
        1 tgctggataa aaactatatc agcgagaatt acattcaggc aattaaagat tccaccatta
       61 acaatggccc ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg cgaccggaat
      121 gtggggcgct taaaaccggg atgtctttga cattacttga acaaggtgtt tattttccgg
      181 ggaatgacga accaattaaa ttactcatcg gactctctgc tgccgatgcc gattcgcaca
      241 ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga agaaatactc gaacaactct
      301 taacagcatc atcagaaaaa caattagcgg acattatcag ccgcggataa ttactctctc
      361 ctctacagga taatattatg gaaaacaagt ctgctcgtgc aaaggtccag gcttttgggg
      421 gatttttgac ggcaatggtc atccccaata taggtgcttt tattgcctgg ggcttcataa
      481 ctgcgttatt tattcccacc ggttggctgc ctaacgaaca tttcgccaaa attgtcggcc
      541 cgatgattac ctgtttattg cccgtgatga ttggttctac aggtggtcat ctggtcggcg
      601 gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt ggcgcagaga
      661 tcccgatgtt ccttggctca atgatcatgg gaccgctcgg tgggttggtc ataaaatatg
      721 tcgataaggc actggaaaaa cgcatacctg ccggttttga gatggttatc aataacttct
      781 cattaggtat cgcggggatg ctcctttgtc tgctgggttt tgaagtgatc ggcccggcgg
      841 tgttaattgc caatactttc gtcaaagagt gtattgaggc gctggtacat gcgggttatc
      901 tgcctctgct gccagtcatc aatgaaccgg cgaaagtgct tttccttaat aatgcgatcg
      961 atcagggcgt ctattatccg ctgggaatgc aacaggcttc ggttaacggt aaatccatct
     1021 tctttatggt ggcctctaac ccaggtccgg gcctggggct gctgctggcg tttaccttgt
     1081 ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat gattattcac ttcctcggtg
     1141 ggatccacga actgtatttc ccatatgtgc tgatgaagcc gctgactatt attgccatga
     1201 ttgcgggcgg tatgtctggt acctggatgt ttaacctact ggacggtggt ctggtggctg
     1261 gcccaagtcc ggggtctatc tttgcgtacc tggcactgac gccgaaaggc tcgttcctgg
     1321 cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt tgctattact tcgctgatac
     1381 tgaagatgga aaaaacggtg gaaacggaga gcgaagatga gtttgctcag tcagccaatg
     1441 cggttaaggc gatgaaacaa gagggtgcat tctcgttaag cagggttaag cgtatcgcct
     1501 ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc gaccaccttc cgtaaacgcc
     1561 tggaaaaagc ggggctggca atagaagtaa aacattacgc gatagaaaac gtgcctgcgg
     1621 atgccgatat cgtcgtcact cacgccagtc tggaagggcg cgtgaaacgt gtgacggata
     1681 aaccgctgat attgattaat aactatattg gcgatccaaa actcgacact ttatttaatc
     1741 aattaaccgc cgaacataaa cactgattac agaggtaaaa atgaaaacca aagttgctgc
     1801 tatttatggc aagcgggatg tccgtctgcg cgaatttgaa ctgccagaaa ttaccgataa
     1861 tgaactgtta gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt
     1921 actcggtagt gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg
     1981 gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa
     2041 aggccagcgt tttgtattgc aaccggcgat ggggttacca agcggatatt cagcgggcta
     2101 cagctacgaa tattttggcg gcaatgccac ttatatgatt attcccgaaa tcgccattaa
     2161 tttgggctgc gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggcagagcc
     2221 tatgtgctgc attattggtg cttatcatgc caattatcac accacgcaat atgtttatga
     2281 gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat
     2341 gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt
     2401 ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc
     2461 ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctatcca
     2521 gatgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt
     2581 gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt
     2641 tgccgggccg acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa
     2701 cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc
     2761 ccttagcgcc acaggacagt tacagccgtc gtttatggtg acccatattg gtggcctgga
     2821 tgcggtgcca gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta
     2881 taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga
     2941 tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca
     3001 ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg
     3061 tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa
     3121 accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca
     3181 ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg
     3241 ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg
     3301 gttgatccca ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc
     3361 atggctttcg ctcctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg
     3421 gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc ggacaacttg
     3481 cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat
     3541 aaaccgcgtc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc
     3601 ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat
     3661 gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg
     3721 ttaggcgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg
     3781 gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt
     3841 aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg
     3901 ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga tggcgaatgg ggtgcggacg
     3961 cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga
     4021 tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg
     4081 gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg
     4141 atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca
     4201 gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg
     4261 atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca cttttcccag
     4321 tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga tataacttgg
     4381 gattttatca gcaatgtcaa cagtataact cgcaatgcga cgctctacga tgcgttaaaa
     4441 gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa
     4501 acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat
     4561 taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc
     4621 ataaaccgtt gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg
     4681 tggtttttct ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat
     4741 atctcgcgca acaagacccg gagctgcccg ctattcagac gctcccgatg gatggttttc
     4801 accattacaa tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga
     4861 catttgacgt tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt
     4921 ggccgcagta cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac
     4981 cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg
     5041 cgcaattttg tgatttctcg atctttatca acgcgccagc tactgcgttg cgggagcgtc
     5101 tggtggggcg caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc
     5161 gtaccgacgg gccgaatgtt cgccgggtgc tggaagagag tctgcctgca aatttgacct
     5221 taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc
     5281 ttccagtcgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc
     5341 taccacgctc gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca
     5401 attttcgtca aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc
     5461 caggcaggcg acggcgacca tccgtcgctg cgcactatgc agatccaacg ggtgcgagtt
     5521 ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc
     5581 cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa
     5641 aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt
     5701 caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgggggcg taagaagacc
     5761 tgccattact cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca
     5821 ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca
     5881 gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc
     5941 cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc
     6001 gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggc
     6061 gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag
     6121 gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac
     6181 ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca
     6241 agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag
     6301 agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg
     6361 agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag
     6421 tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca
     6481 aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt
     6541 gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg
     6601 tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg
     6661 cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac
     6721 ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac
     6781 gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa
     6841 taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag
     6901 cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt
     6961 actgcattca caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag
     7021 catgcaacca ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac
     7081 cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc
     7141 gccgccgcgt ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca
     7201 aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc caatgcccac agtgttaatg
     7261 aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa
     7321 tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt
     7381 tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc
     7441 aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg
     7501 gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca
     7561 gggtgcgaaa ccaacccgct cgcgccagcc aaccggcgac caggccgatg accatcgcga
     7621 cgggtgcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta
     7681 agccggtcag cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg
     7741 agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg
     7801 cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa
     7861 aacctctttg aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc
     7921 gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg
     7981 gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa
     8041 acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg
     8101 aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc
     8161 aggctaccgg tacagcctgc aatccaggcg gcatgaagtt gatgttgctg tgcaaaagcg
     8221 tgtagctggg aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag
     8281 gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg
     8341 ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac
     8401 gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg
     8461 gggctgaaaa cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt
     8521 tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta
     8581 accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct
     8641 gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat
     8701 aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg
     8761 ggcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt
     8821 gttgaaatat gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca
     8881 actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc
     8941 ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga
     9001 gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag
     9061 taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca
     9121 ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt
     9181 agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga
     9241 tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc
     9301 gatcattccg gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa
     9361 cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt
     9421 aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc
     9481 agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga
     9541 ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc
     9601 acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct
     9661 cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa
     9721 aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc
     9781 ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag
     9841 taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag
     9901 gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag
     9961 aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc
    10021 tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa
    10081 acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg
    10141 cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta
    10201 ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac
    10261 tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg
    10321 gttctaaagt atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc
    10381 tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta
    10441 aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca
    10501 tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc
    10561 tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg
    10621 ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg
    10681 gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaat gctatcgcag
    10741 acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg
    10801 gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg
    10861 aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca
    10921 cagggcaggg aaacctgccc ttgtttcagc gcgcttttag agcacgcatt ttttcatact
    10981 ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccctaggag cttacataag
    11041 taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat
    11101 aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc
    11161 gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca
    11221 ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct
    11281 aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt
    11341 aaaggcgtga aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg
    11401 catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac
    11461 tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc
    11521 gcagctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg
    11581 caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact
    11641 ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg
    11701 gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc
    11761 aaaatcagcc cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag
    11821 ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag
    11881 aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact
    11941 gctcaggaaa tcaaagactc cgtaagctac ggcgtagtga aaatgaatat cgataccgat
    12001 acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg
    12061 cagggtcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg
    12121 cgcgtatggc tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag
    12181 gaactgaacg cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc
    12241 tgcgggtctt tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg
    12301 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    12361 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    12421 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    12481 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    12541 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    12601 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg
    12661 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    12721 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    12781 cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    12841 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    12901 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    12961 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    13021 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    13081 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    13141 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    13201 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    13261 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    13321 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    13381 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat
    13441 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    13501 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    13561 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg
    13621 cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    13681 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    13741 gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag
    13801 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    13861 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    13921 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    13981 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    14041 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa
    14101 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg
    14161 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    14221 tccggtacgg caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac
    14281 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    14341 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    14401 acccagccag attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg
    14461 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    14521 ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc
    14581 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    14641 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    14701 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    14761 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    14821 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    14881 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    14941 aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac
    15001 ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg
    15061 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
    15121 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
    15181 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
    15241 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
    15301 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
    15361 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
    15421 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
    15481 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
    15541 attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
    15601 gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
    15661 gaatatgcga ccttgtggga tcaatctcac cattatttat cgtccctaca gttttatggc
    15721 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
    15781 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
    15841 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
    15901 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
    15961 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
    16021 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
    16081 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
    16141 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
    16201 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
    16261 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc
    16321 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
    16381 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
    16441 gttgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
    16501 gattttgccg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
    16561 gaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
    16621 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
    16681 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
    16741 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
    16801 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
    16861 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
    16921 aagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
    16981 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
    17041 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
    17101 actcaccgcg ccctgattga tctttttacg taaactggac gatgtttgat atggcgcacg
    17161 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
    17221 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
    17281 aatcgcggcg ggtagggctt tcggcgaacc cgctggggta aagccgctaa atgccaccat
    17341 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
    17401 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
    17461 aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg gcgcatcccc
    17521 tgaatacgtt caaattcatc ggagttcatg gtgtgtgctt cgcccagtac acgcagctct
    17581 tctttgataa cggcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
    17641 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
    17701 cgctgggcgg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
    17761 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
    17821 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
    17881 gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
    17941 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
    18001 agttcgtgaa tatcgtgacc tgcggagaag actttggagc cacttggcgc ccgcaaaatg
    18061 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatt atcaataaag
    18121 actttactta aggcattaag ttttcgtcca tagttaaact caatgaccgc cactttgttg
    18181 atagtggcaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
    18241 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
    18301 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
    18361 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
    18421 taaacgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc agatctcatc
    18481 gattccacgt ttttccagtg cgctacaagt cagaccccgt ggctgccatt cgtcgtattt
    18541 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
    18601 gttatcgcta tcgtctttgt tgataacgag cagatcagcc acttccatca gccctttttt
    18661 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
    18721 gcgggcgact tccgtttccg actgcgcgac gccaaccgtt tcgacaatca ctacgtcata
    18781 acccgccgct tcgcataaca gcattaattc ccgtgctcgc tgactggcac cgcccagatg
    18841 accggaggag ggtaccgggc gaataaacgc cgcctcggca cgcgccaggt cattcatgcg
    18901 ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
    18961 agcaaccttt aatccctcac gaatcaacaa catgccaaag gcttcaagaa aggtactttt
    19021 ccccgcgccg ggtgtgccgg taacgcccag tcgcagggcg ttaccacaga acggcataat
    19081 ggcgtcaagc agctgtgtac ttagcgcctg atgacgtggg tgacggcttt ccaccagcat
    19141 catggcttgg gcgagcgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag
    19201 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
    19261 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
    19321 cctgcggcgg aatgacgcca cccgcgacca cgcagatatc ttcgcgtccc cattttttca
    19381 gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
    19441 ctacgtgaac gtcgttttct acggccaggc gggcgatctc ttcaggtgta gagaacatcg
    19501 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgcgccgc
    19561 gatcgtgtcc atcctggccc atcttagcga tcagaatgcg cgggcgacga ccattgtcgg
    19621 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagact
    19681 gatgatagct ttgcgcaatc acgccggtaa cacactggca ccagataacg gtcgaaagcg
    19741 acttccagcg catcggaaat ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg
    19801 gcagccagca ggttttcgtt atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt
    19861 acggcggcat catcacgggt ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc
    19921 accatcacgt tgtcgatctc aagtacatcg gtttcgtctt cgtgatccag tttgtacttg
    19981 ttgacaccaa cgatgacacg cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc
    20041 tcttcgatca ttcgttttgg cagacctgct tcgatcgctt tcgccatgcc accggcttcg
    20101 tcgatctgtt ggataatagc tctggcttgt ttgacgattt gatcggtcag cgactcaatg
    20161 taataggatc cggccagtgg atcgacggtg tggcagagtt ctgattcttc ctggatgatg
    20221 atctgggtgt tgcgggcaat gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca
    20281 aaggcgttgg tatgcagtga ctgagtaccg cccagcgtcg cagccagtgc ttcaatggtg
    20341 gtgcggataa cgttgttata cggatcctgt tcagtcaggc tccagcctga ggtctggcag
    20401 tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa atccactgac cgcttcgctc
    20461 cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat gccgataccg
    20521 aagaataacg acaggcgagg agcgaagtca tcaattttca gtccggcaga gattgctgct
    20581 ttgatgtact caatcccatc ggcgagcgta aatgctacct gctgcacgca gttggcaccc
    20641 gcttccccca tgtggtaacc gctgatactg atggtattaa atcgcggcat gttgccggaa
    20701 caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggtgggta aatataggtg
    20761 ttgcggcaga ggtactcttt gagaatatcg ttttgaatgg tgccggtcag tttatcaggt
    20821 gtaacacctt gctcttctgc ggcgacgata taaaacgcca gtactggtag cactgcgcca
    20881 ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag gactttcata
    20941 tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cacccgccac gcgtgggtta
    21001 tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg
    21061 gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa accagcatac
    21121 tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg aacgtagggc
    21181 ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc ggtatacagc
    21241 ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt tttctcccga
    21301 cggctcaatt ccttgttggc aagctgttgc cactcctgct cgttagacat tatttgctcc
    21361 gtaaaacgca atcaaaatga gggtatttgc gcttacggtg agctttttac gtgggtttgt
    21421 cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt gtctattttg
    21481 tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt gctatcaggc
    21541 attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt aacgcatcag
    21601 tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc atccgtcgtt
    21661 gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc tcgatttgca
    21721 ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag
    21781 aattaacgat atggcagggt acgctgccag gaggaagatc gaagttttgc tgcaaaaagg
    21841 cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcaat aatgccgaac
    21901 gcgttacgcc gttaggaaaa tatttttcgg caaacggttt tgagctgacg aacagatagt
    21961 cgagcgcacc gagtttatcg acaagacaac tcggcagcgc ctgatgttga atactcaccg
    22021 cgccgaccac ttcgccgcgg cgcagacgtt cctgagtacg ggtttcatct tctacctgca
    22081 agttgaggcg gataggcgaa tcagccaaca caggagccag tgccggaagc aaccacgtcg
    22141 ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat
    22201 cgcccagcca ctcttcttcc agcaactcca cttggcgcag cagtgccagc agtttttgcc
    22261 cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg aacatatttt
    22321 ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt tgtgccgcgc
    22381 gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg tagtccgggc
    22441 gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga cacaattttg
    22501 tgtcaggttg catataccga agccgtcggc gggtaacatt tgtatcaatg gttaaacgcg
    22561 cagcttaaaa gatcagggat ttgcgctatt gcatttcgct tagcgacttc gcgccctggt
    22621 tatgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca tacccagacc
    22681 gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat attttatgga
    22741 tgagttaacc acccggcttg ccaacggggt ctgaatcgct ttttttgtat ataatgcgtg
    22801 tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa aaagcagtag
    22861 gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt acaggttcca
    22921 ccgccgcaca ctttattgac gcgctcggca cgatgaaagg ccagattgaa ggggccgttt
    22981 ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt tttgatctca
    23041 acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac ggccacatgc
    23101 aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct tcggttgcgg
    23161 aaaaatttat ctgtattgcc gacgcctcca agcaggttga tattctgggt aaattcccgc
    23221 tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgccagctg gtgaaactgg
    23281 gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg atcctcgacg
    23341 tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat gcgattcctg
    23401 gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg attggcacac
    23461 ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc gttaaaaaaa
    23521 ttctcttcat taaatttggt gacatgtgtc acgcttttag caggcaattg tcgattgctc
    23581 taaataaatc ctctaaacca gcatattcat ccaagaatta cctttgcgtg atatttcctc
    23641 aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg ttgaaaggcg
    23701 gatgcaaatc cgcacacaac atttcaaaag acaggattgg gtaaatggca aaggtatcgc
    23761 tggagaaaga caagattaag tttctgctgg tagaaggcgt gcaccaaaag gcgctggaaa
    23821 gccttcgtgc agctggttac accaacatcg aatttcacaa aggcgcgctg gatgatgaac
    23881 aattaaaaga atccatccgc gatgcccact tcatcggcct gcgatcccgt acccatctga
    23941 ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc tgtatcggaa
    24001 caaaccaggt tgatctggat gcggcggcaa agcgcgggat cccggtattt aacgcaccgt
    24061 tctcaaatac gcgctctgtt gcggagctgg tgattggcga actgctgctg ctattgcgcg
    24121 gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg gcggcgggtt
    24181 cttttgaagc gcgcggcaaa aagctgggta tcatcggcta cggtcatatt ggtacgcaat
    24241 tgggcattct ggctgaatcg ctgggaatgt atgtttactt ttatgatatt gaaaacaaac
    24301 tgccgctggg caacgccact caggtacagc atctttctga cctgctgaat atgagcgatg
    24361 tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc gcgaaagaga
    24421 tttcgctaat gaagcccggc tcgctgctga ttaatgcctc gcgcggtact gtggtggata
    24481 ttccggcgct gtgtgatgcg ctggcgagca aacatctggc gggggcggca atcgacgtat
    24541 tcccgacgga accggcgacc aatagcgatc catttacctc tccactgtgt gaattcgaca
    24601 acgtccttct gacgccacac attggcggtt cgactcagga agcgcaggag aatatcggcc
    24661 tggaagttgc gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc tctgcggtga
    24721 acttcccgga agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac atccacgaaa
    24781 accgtccggg cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc gtcaacatcg
    24841 ccgcgcaata tctacaaact tccgcccaga tgggttatgt agttattgat attgaagctg
    24901 acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt accattcgcg
    24961 cccgtctgct gtactaattc cccttctctg aaaattaacg ggcaggtcac tgacttgccc
    25021 gtttttttat cccttctcca caccgttgcc gcgttgcgca ttctactttg cgagtctgct
    25081 ccagaaaata aatcaaagtc agatcaatgc gttgtgttta tttaatgggg atttacgatg
    25141 gcgggacagc atggggctgt aacggtaaag ccctcaccga agcgagggct taaaggagaa
    25201 gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt ttcggagctt
    25261 attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac taaattaggt
    25321 caaagaatca acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa ccgaactttt
    25381 tgatagccac ttcatcgccc acgtttactg ccgtaataga tcctttttcc caaaaattat
    25441 ctctgttttg catggggttt ttcgagcacg ctcgaagaaa agatttaaag gtagatcaat
    25501 gcgttgtggt tatttaaaga gagcttacga tagctgggca gcatggggct gtaacggtaa
    25561 agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg ccatcatact
    25621 gattgtactg ttactcataa gtttcccagc ttattaacag tcagtcacag gggaggagca
    25681 atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc aatcagggcg
    25741 atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac cgcaggaaga
    25801 gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg cgcataaccc
    25861 accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa accaccgccc
    25921 attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag cacgtctaat
    25981 cgggaaaggg gtagcacgtc acgcacatcc agttttggct catggatctt caacctgttc
    26041 atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc actaaaggga
    26101 tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat tagcggctgg
    26161 gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat caccaccggg
    26221 ggataagtca tcatccgggt agcggcttgt tgacccattt ccagctgttg ttccggcgtt
    26281 aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa tgtcagtggg
    26341 agctctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg agatgccgcc
    26401 gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag ttttaaggct
    26461 tctcggacga accgagcatg ctcaccaacc gcggagcgcc acattcttgt ggtatgaaat
    26521 atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc ttcacggtta
    26581 ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt tcggtgatgc
    26641 gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata cgctgttcca
    26701 tacttgccgc gtagtcacga gtctttgctt tttcttgcgc taactcatag ctgatattca
    26761 atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct ttcagatctt
    26821 gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg tcaggcgggc
    26881 agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca gacatgccac
    26941 cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg aaggggcgac
    27001 actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg gcccaaagtg
    27061 gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat acagaacgaa
    27121 atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg tctgacccct
    27181 gccgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga cagctcatgg
    27241 ctgccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga gctggcacag
    27301 gcactgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg cttccttttt
    27361 cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga tgcactggca
    27421 ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct ggacaaagtg
    27481 accggcgaaa ccggtgaagc catcgacgat ctgcgtaaca tcgcgcagct gggctacgac
    27541 gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga atacgttcgt
    27601 gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc gccagaagta
    27661 caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga gtgagatatc
    27721 ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac ccggcagcgc
    27781 cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat acccctatcg
    27841 tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg tgctggtgct
    27901 gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc gcgacctgac
    27961 ggcggagatc tggtttggcc gtcgcttagg ccaggatgcc gcgccagaga aactgggcgt
    28021 tgaccgcgca ctggcattca gcgaaatcaa tcagcaactt tatcaactac ttaacggcct
    28081 ggatgtggtt taccatgccc aggacgaata tgcatatgct gatgaaatcg tgaacagtgc
    28141 gctggaaaaa ctgcgtaaag gttcgcggca aaatctcacc gcaccggcaa cgatgatcga
    28201 ctggcgtcct gttgttcatg aaatgcgcct gttcaaatcg ccagaagaga ttgccgtact
    28261 ccgccgcgcg ggagaaatca ccgccatggc acatacacgg gcgatggaaa aatgccgtcc
    28321 gggaatgttc gagtaccatc tggaaggcga aattcaccac gaatttaacc gccacggtgc
    28381 gcgctatcct tcctataaca ccattgtagg cagcggtgaa aacggctgca ttctgcacta
    28441 caccgaaaac gagagtgaac tgcgcgacgg tgacctggtg ttgattgacg cgggttgtga
    28501 atacaaaggt tacgcgggcg atattacccg caccttcccg gtcaacggca aattcaccca
    28561 ggcccagcgt gaaatctacg acattgtgct ggagtctctc gaaaccagcc tgcgcctgta
    28621 tcgtccggga acttccattc tggaagtcac tggtgaagtg gtgcgcatca tggttagcgg
    28681 cctggtaaaa ctcggcatcc tgaaaggtaa tgttgatgaa ctgatcgctc agaacgccca
    28741 tcgtcctttc tttatgcatg gccttagcca ctggttagga ctggatgtcc atgacgttgg
    28801 cgtttatggt caggatcgct cgcgcattct ggaaccgggg atggtactga ccgtagagcc
    28861 tggtctgtat atcgcgccgg atgcggatgt gccagaacaa tatcgcggta tcggcattcg
    28921 tattgaagac gacattgtga ttaccgaaac cggcaacgaa aacctcaccg ccagcgtggt
    28981 gaaaaagccg gaagaaatcg aagcgttgat ggctgctgcg agaaagcaat gagcgtaatc
    29041 atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg gttaagtcac
    29101 ggggcgctgc cggtacattt gattgaagcg actgcgccag agtcacatgc tcatccgggc
    29161 tttgatggac gagcgatagc gctggcggcg ggtacctgtc agcaactggc gcgcatcggc
    29221 gtctggcaat ctctggctga ttgcgcaacc gccatcacta ccgtgcatgt cagcgatcgt
    29281 ggtcatgctg gattcgtcac cctcgtcgca gaagattacc aactggcggc gctggggcag
    29341 gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat tgctgcgaaa agcacctggc
    29401 gtaatgctgc attgccccga tcgcgtagct aacgttgccc gtactcagag tcacgttgaa
    29461 gtgacgctgg agagcggcga gatgctgacg ggccgcgtgc tgatagccgc tgatggcacc
    29521 cattcagcgt tagccaccgc gtgcggcgtt gattggcagc aggagcctta cgaacaactg
    29581 gcagtgattg ccaacgttgc tacttctgtt gcgcacgaag gccgcgcttt tgaacgcttc
    29641 acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc gctggtctgg
    29701 tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa gttttgccgt
    29761 gaactccagt ctgcctttgg ctggcgactg gggcaaatta cccacgccgg taaacgcagt
    29821 gcttatccgc tggcattaac ccgcgccgcc agaccgatta cccatcgcac tgtgctggtg
    29881 ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag ggtttaacct cggtatgcga
    29941 gatgtcatga gccttgcgga aaccctgact caggcgcatg agcgcggaga agacatcggt
    30001 gattacggca tattgtgccg ctatcagcag cgtcgacaga gcgatcgcga agcaaccatc
    30061 ggcgtcacgg acagcctggt acatcttttt gccaaccgtt gggcaccgct ggttgtcggg
    30121 cgcaacatcg gactgatgac gatggaatta ttcaccccgg cacgcgatgt gctggcgcag
    30181 cgcaccctcg gttgggtggc gcgttgaagg cggttttaag gaataaacaa tgcaaagtgt
    30241 tgatgtagcc attgttggcg gcggcatggt ggggctggcg gttgcctgcg gcatacaggg
    30301 gagcggctta cgcgttgccg tactggagca gcgcgtaccg gaacctctgg cggtagatgc
    30361 actaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac tcacccgtct
    30421 tggcgtctgg caggacattc tcgctcgtag ggctagttgc tatcacggca tggaagtgtg
    30481 ggacaaagac agttttggtc acatctcgtt tgacgatcaa agcatgggct atagccatct
    30541 tgggcacatc gttgaaaact cagtgattca ctacgcgctg tggaacaaag cgcagcagtc
    30601 gtcagatatt actctgttgg cccccgcaga attacagcag gtcgcctggg gagaaaatga
    30661 aaccttcctg acgctgaaag atggcagcat gttaacggcg cgtctggtga ttggcgcgga
    30721 cggtgctaat tcctggttgc gcaacaaagc agatattccg ctgactttct gggattatca
    30781 gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg catgatgcgg tggcgcggca
    30841 ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc atctttgctc
    30901 gattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa gtgaagacga
    30961 atttaatcgc gcgttaaata tcgcttttga taatcgcctt ggcttatgca aggttgagag
    31021 cgcgcgtcag gtgttcccgc tgacggggcg ttatgcgcgc cagtttgccg cacaccgtct
    31081 ggcgctggtg ggcgacgccg cgcataccat tcacccactg gcggggcagg gcgttaatct
    31141 cggatttatg gacgccgcag agctgattgc tgaactgaaa cggttgcatc gtcagggtaa
    31201 agacatcggg cagtacattt acctgcgccg ctatgaacgt agccgcaagc acagcgcggc
    31261 gctgatgctg gctggtatgc agggttttcg cgatctcttt tccggcgcta atccggcgaa
    31321 aaaactgctg cgtgatattg gtttgaaact ggccgacacg cttcctggcg ttaagccgca
    31381 acttatccgc caggcaatgg gattaaacga tttgcctgaa tggctgcgtt aaaaatttct
    31441 cctctgttgt ttatttgata cccatcacac tttcatctcc cggttttttc gccgggagat
    31501 tttcctcatt tgaaataaac taatttcacc tccgttttcg cattatattt tctaatgcca
    31561 tgattttttg atttagtgtt ttttgacatt tttttagctc ttaatattgt cttatccaaa
    31621 ttgactttct catcacatca tctttgtata gaaattggtg tattttttgg ttttttattc
    31681 tgtcgcgatt tttgcatttt ttaaccataa gctaatgtga tgatcaattt taccttatgg
    31741 ttaacagtct gtttcggtgg taagttcagg caaaagagaa cgattgcgtt ggggaccggg
    31801 agtggctccg atgctgggtt ttgtggtgat aatttcacca tgaaaaagtt gtcagccccg
    31861 cttattcaat gaggacaaga tggcacaaca gactcctttg tacgaacaac acacgctttg
    31921 cggcgctcgc atggtggatt tccacggctg gatgatgccg ctgcattacg gttcgcaaat
    31981 cgacgaacat catgcggtac gtaccgatgc cggaatgttt gatgtgtcgc atatgaccat
    32041 cgtcgatctc cgcggcagcc gcacccggga gtttctgcgt tatctgctgg cgaacgatgt
    32101 ggcgaagctc accaaaagcg gcaaagccct ttactcgggg atgttgaatg cctctggcgg
    32161 tgtgatagat gatctcatcg tctactactt tactgaagat ttctttcgcc tcgttgttaa
    32221 ctccgccacc cgcgaaaaag acctctcctg gattacccaa cacgctgaac ctttcggcat
    32281 cgaaattacc gttcgtgatg acctttccat gattgccgtg caagggccga atgctcaggc
    32341 aaaagctgcc acactgttta atgatgctca gcgtcaggcg gtggaaggga tgaaaccgtt
    32401 ctttggcgtg caggtgggcg atctgtttat tgccaccact ggttataccg gtgaagcggg
    32461 ctatgaaatt gcgctgccca atgaaaaagc ggccgatttc tggcgtgcgc tggtggaagc
    32521 gggcgttaag ccctgcggtc tgggggcgcg tgacacgctg cgtctggaag cgggcatgaa
    32581 tctttatggt caggagatgg acgaaactat ttctccttta gccgccaaca tgggctggac
    32641 tatcgcctgg gaaccgacag atcgtgactt tatcggtcgt gaagccctgg aagtgcagcg
    32701 tgagcatggt acagaaaaac tggttggtct ggtgatgacc gaaaaaggcg tgctgcgtaa
    32761 tgaactgccg gtacgcttta ccgatgcgca gggcaaccag catgaaggca ttatcaccag
    32821 cggtactttc tccccgacgc tgggttacag cattgcgctg gcgcgcgtgc cggaaggtat
    32881 tggcgaaacg gcgattgtgc aaattcgcaa ccgtgaaatg ccggttaaag tgacaaaacc
    32941 tgtttttgtg cgtaacggca aagccgtcgc gtgatttact tttttggaga ttgattgatg
    33001 agcaacgtac cagcagaact gaaatacagc aaagaacacg aatggctgcg taaagaagcc
    33061 gacggcactt acaccgttgg tattaccgaa catgctcagg agctgttagg cgatatggtg
    33121 tttgttgacc tgccggaagt gggcgcaacg gttagcgcgg gcgatgactg cgcggttgcc
    33181 gaatcggtaa aagcggcgtc agacatttat gcgccagtaa gcggtgaaat cgtggcggta
    33241 aacgacgcac tgagcgattc cccggaactg gtgaacagcg aaccgtatgc aggcggctgg
    33301 atctttaaaa tcaaagccag cgatgaaagc gaactggaat cactgctgga tgcgaccgca
    33361 tacgaagcat tgttagaaga tgagtaacgg ctttattcct cttctgcggg agaggatcag
    33421 ggtgaggaaa atttatgcct caccctcact ctcttcgtaa ggagagaggt tcacaattca
    33481 ctgcacgttt caggaaccat cgctcatgac acagacgtta agccagcttg aaaacagcgg
    33541 cgcttttatt gaacgccata tcggaccgga cgccgcgcaa cagcaagaaa tgctgaatgc
    33601 cgttggtgca caatcgttaa acgcgctgac cggccagatt gtgccgaaag atattcagct
    33661 tgcgacacca ccgcaggttg gcgcaccggc gaccgaatac gccgcactgg cagaactcaa
    33721 ggctattgcc agtcgcaata aacgcttcac gtcttacatc ggcatgggtt acaccgccgt
    33781 gcagctaccg ccggttatcc tgcgtaacat gctggaaaat ccgggctggt ataccgcgta
    33841 cactccgtat caacctgaag tctcccaggg ccgccttgaa gcactgctca acttccagca
    33901 ggtaacgctg gatttgactg gactggatat ggcctctgct tctcttctgg acgaggccac
    33961 cgctgccgcc gaagcaatgg cgatggcgaa acgcgtcagc aaactgaaaa atgccaaccg
    34021 cttcttcgtg gcttccaatg tgcatccgca aacgctggat gtggtccgta ctcgtgccga
    34081 aacctttggt tttgaagtga ttgtcgatga cgcgcaaaaa gtgctcgacc atcaggacgt
    34141 cttcggcgtg ctgttacagc aggtaggcac taccggtgaa attcacgact acactgcgct
    34201 tattagcgaa ctgaaatcac gcaaaattgt ggtcagcgtt gccgccgata ttatggcgct
    34261 ggtgctgtta actgcgccgg gtaaacaggg cgcggatatt gtttttggtt cggcgcaacg
    34321 cttcggcgtg ccgatgggct acggtggccc acacgcggca ttctttgcgg cgaaagatga
    34381 atacaaacgc tcaatgccgg gccgtattat cggtgtatcg aaagatgcag ctggcaatac
    34441 cgcgctgcgc atggcgatgc agactcgcga gcaacatatt cgtcgtgaga aagcgaactc
    34501 caacatttgt acttcccagg tactgctggc aaacatcgcc agcctgtatg ccgtttatca
    34561 cggcccggtt ggcctgaaac gtatcgctaa ccgcattcac cgtctgaccg atattctggc
    34621 ggcgggcctg caacaaaaag gtctgaaact gcgccatgcg cactatttcg acaccttgtg
    34681 tgtggaagtg gccgacaaag cgggcgtact gacgcgtgcc gaagcggctg aaatcaacct
    34741 gcgtagcgat atcctgaacg ccgtggggat cacccttgat gaaaccacca cgcgtgaaaa
    34801 cgtaatgcag cttttcagtg tgctgctggg cgatagccac gggctggaga tcgacacgct
    34861 ggacaaagac gtggctcacg acagccgctc tatccagcct gcgatgctgc gcgacgacga
    34921 aatcctcacc catccggtgt ttaatcgcta tcacagcgaa accgaaatga tgcgctatat
    34981 gcactcgctg gagcgtaaag atctggcgct gaatcaggcg atgatcccgc tgggttcctg
    35041 caccatgaaa ctgaacgccg ccgccgagat gatcccaatc acctggccgg aatttgccga
    35101 actgcacccg ttttgcccgc cggaacaggc tgaaggttat cagcagatga ttgcgcagct
    35161 ggctgactgg ctggtgaaac tgaccggtta cgacgccgtt tgtatgcagc cgaactccgg
    35221 cgcacagggc gaatacgcgg gcctgctggc gattcgtcat tatcatgaaa gccgcaacga
    35281 agggcatcgc gatatctgcc tgatcccggc ttctgcgcac ggaactaacc ccgcttctgc
    35341 acatatggca ggaatgcagg tggtggttgt ggcgtgtgat aaaaacggca acatcgatct
    35401 gactgatctg cgcgcgaaag cggaacaggc gggcgataac ctctcctgta tcatggtgac
    35461 ttatccttct acccacggcg tgtatgaaga aacgatccgt gaagtgtgtg aagtcgtgca
    35521 tcagttcggc ggtcaggttt accttgatgg cgcgaacatg aacgcccagg ttggcatcac
    35581 ctcgccgggc tttattggcg cggacgtttc gcacctcaac ctgcataaaa ctttctgcat
    35641 tccgcacggc ggtggtggtc cgggtatggg accgatcggt gtgaaagcgc atctggcacc
    35701 gtttgtaccg ggtcatagcg tggtgcaaat cgaaggcatg ttaacccgtc agggcgcagt
    35761 ttctgcggca ccgttcggta gcgcctctat cctgccaatc agctggatgt acatccgcat
    35821 gatgggcgca gaagggctga aaaaagcaag ccaggtggca atcctcaacg ccaactatat
    35881 tgccagccgc ctgcaggatg ccttcccggt gctgtatacc ggtcgcgacg gtcgcgtggc
    35941 gcacgaatgt attctcgata ttcgcccgct gaaagaagaa accggcatca gcgagctgga
    36001 tattgccaag cgcctgatcg actacggttt ccacgcgccg acgatgtcgt tcccggtggc
    36061 gggtacgctg atggttgaac cgactgaatc tgaaagcaaa gtggaactgg atcgctttat
    36121 cgacgcgatg ctggctatcc gcgcagaaat tgaccaggtg aaagccggtg tctggccgct
    36181 ggaagataac ccgctggtga acgcgccgca cattcagagc gaactggtcg ccgagtgggc
    36241 gcatccgtac agccgtgaag ttgcggtatt cccggcaggc gtggcagaca aatactggcc
    36301 gacggtgaaa cgtctggatg atgtttacgg cgaccgtaac ctgttctgct cctgcgtacc
    36361 gattagcgaa taccagtaat tcactgattc gagtattttc taaaggcgct tcggcgcctt
    36421 tttagtcaga tgacaaagta caaaagtgct cagacagtcc cctcgcccct ttggggagag
    36481 ggttagggtg aggggaacag gccggcactg gcgcgaatat ttaccctcac cccgaccctc
    36541 tccctgaaag ggcgaggggg aaaagcgtgc caacattgaa gattgagcca gtttgttagc
    36601 aatctcaaag atacgtcaac gaattaattt ttctcggaaa aacaaatggc tatagcactt
    36661 gtgaccggtg gcagttgcgg catcgggcgg gcaactgcat tactgttggc gcaagaaggg
    36721 tatacggtgg cggttaatta tcagcaaaac ctccacgcgg cgcaggaagt gatgaactta
    36781 ataatgcaag ccggtggcaa agcattcgtg ctccaggcgg atatcagcga cgaaaaccag
    36841 gtcgttgcga tgtttacagc aatcgatcag cacgatgaac cgctagcagc gctggtcaat
    36901 aacgccggga tcttgtttac ccagtgcacc gttgaaaacc ttaccgcaga gcgaatcaac
    36961 cgagtacttt ccaccaacgt gacgggatat tttctctgct gccgcgaggc ggtaaaacgc
    37021 atggcgctta aaaatggtgg cagtggcggc gctatcgtca atgtctcttc ggtggcctca
    37081 cgattgggtt cgccagggga atatgttgat tacgcggcat cgaaaggggc gattgatacg
    37141 ttaaccaccg gactatcgct ggaagtcgcc gcgcagggga tccgcgttaa ctgcgtgcgg
    37201 ccagggttta tttataccga aatgcacgcc agcggcggcg agcctggacg cgtcgatcgc
    37261 gttaagtcga acatccccat gcagcgtggt ggacaggcag aagaggtcgc gcaggccatt
    37321 gtctggctac taagtgataa agcctcttac gtcacgggaa gttttatcga tttggcgggc
    37381 gggaaataaa atagggaagt tgtctgaccg gatgcaacaa gtattgcatc cggtacttca
    37441 tcgacttaaa gcttctcgcc gttgctggca atcacctctt tgtaccagtt aaagctcttc
    37501 ttacgtgaac gcgacatatc gccagtaccg tcgtcatgtt tattcacata gataaagccg
    37561 tagcgtttgc tgtactgccc ggtagtgaat gacacgcagt cgatgcagcc ccacggtgtg
    37621 tagcccatca gatccacacc atcgtaagtc actgcttttt tcatctcttc aatatgggcg
    37681 cgcaggtagt caatgcggta gtcgtcgttg atgctgccat cttcttccac tttgtcgtaa
    37741 gcgccaaaac cgttttcgac aataaacagc ggcctctgat aacgctcata cagttcgcaa
    37801 agggcatagc gcagacctac tggatcaatc tgccagcccc agtcagacgc tttaacatac
    37861 gggttcggta cgctgccttc aaaaccagag atcgcatcgc cggtgccgcc ttcggccttc
    37921 actgcgttgg tcatgtaata gctgaaacca agataatcgc aggtgccttc gcgcagtaca
    37981 tcgagatcgc cgtcttccat tttgatatta aatccgcgac gttcccactc gttcaataca
    38041 taggacgggt aatagccgca tagctgcaca tcggtaaaga cgtagcgttc gcgcatcgac
    38101 tcctgagcga acatcacatc gtccgggtta catgagtagg gatagagcgg caccatcgcc
    38161 agcatacagc cgactttcat ctccgggtta atgcgacgcg cggctttcac tgccagggcg
    38221 ctggcgacaa actggtgatg cagcacctgg tacatcgtct cttccgggtt gtcatgctcg
    38281 gtatacacca cgccggagca gcagtaacca aacagcggcg cacgccagtt acgctggttg
    38341 tttatttcgt tgaaggtcat ccagtatttg actttatgct tgtagcgttc gaataccact
    38401 tccgcgaaac ggacaaagaa atcaacgact ttacggttgg tccagctacc gtattgctgc
    38461 accagatgca gcggcatttc aaagtgggag agggttatca ccggttcgat gttgtatttc
    38521 agcagttcat cgaacagaga gtcgtaaaac ttcagccctt cttcgtttgg ctgagcttca
    38581 tcaccttttg gaaaaatacg cgtccaagcg atggaagtcc gaaaacattt gaagcccatt
    38641 tcggcaaata gcttgatgtc ttccttgtag tgaccataaa aatcaacggc ttcatggttt
    38701 ggatagtatt ttcctggcaa gacttctttg gtgatttcgc gcggcacgcc gtgtgcgcca
    38761 ccggtcagaa cgtcacaaat gctcggtcct ttgccgcctt tgttccagcc gccttcgacc
    38821 tgatgagcgg caacggcgcc accccataag aaatctttcg gtaaggtgag ttttttcact
    38881 atcataggct ccagatcagt caattcttac aggaaatagt accatgcagc caaacgacat
    38941 cacttttttt caacgtttcc aggatgacat tctggctggg cgtaaaacca tcaccatccg
    39001 cgacgagtct gaatcgcact tcaaaacggg tgatgtgctt cgtgtcggac gttttgaaga
    39061 tgacggttat ttttgcacga ttgaagtcac cgcaacctca accgtaacgc tggatacact
    39121 gacggaaaaa catgcagagc aggaaaatat gaccctgcct gaactgaaaa aggtcattgc
    39181 tgacatctat cccggtcaga cacaatttta tatgattgaa tttaaatgtc tttaaattga
    39241 actcacgcct gttagttgaa atttcgcatt taatttcatg atttttagga gattagtctc
    39301 aaatcagaat ttattttagc taacaggtgt tcactggaac tattctcagt tacgctggag
    39361 aggtatacat gtgcgtgtac gtttctctgg agtaagttat ggttcagaag cccctcatta
    39421 agcagggata ttcgctggca gaggaaattg ccaacagcgt cagtcacggc attgggctgg
    39481 tgtttggtat cgttgggctg gtgttgctac tggttcaggc ggtggatctc aatgccagcg
    39541 ccacagcgat aaccagttac agcctctatg gcggcagtat gatcctgctg ttcctcgctt
    39601 cgacgctcta tcacgccatt cctcatcaac gggcaaaaat gtggctgaag aaatttgacc
    39661 attgcgctat ttacctgttg attgccggaa cctacacgcc gtttttgctg gtggggctgg
    39721 attctccgtt agcgcgcggg ttgatgattg ttatctggag cctggcattg ctggggattc
    39781 tgttcaaact gaccatcgcg caccgattca aaattttatc tctggtgact tatctggcga
    39841 tgggctggct gtcgctggtg gtaatttatg aaatggcagt taagctcgcg gcgggcagcg
    39901 ttaccttact ggcggtaggc ggcgtggttt attcgctcgg ggtgattttc tacgtctgca
    39961 aacgcattcc atacaaccat gccatctggc acggcttcgt gctcggcggt agtgtgtgcc
    40021 actttctggc gatctatttg tatattgggc aggcgtaatg ggtatgcctt tgtaggcctg
    40081 ataagacgcg tcagcgtcgc atcaggcatc ggagcgcaac cgccggatgc ggcgtgaacg
    40141 ccttatccga tctacgggcc tcgtccctgt tgtgataaag gcatcaaatc ccagacatga
    40201 taagacgcgt cagcgtcgca tcaggcgata aatgcctgat gcgatacaga gatttactct
    40261 tcgagcgaat acggcagcgg ctcgatatgc agtgtattcg catcgtcgcg tacgcggaag
    40321 atgctgtccg gttccatatc gttattcatg acgacctgta ccacgacctg accatcttcc
    40381 agttttaccg cagccagcgc cgtaccggta cgacgccagt tctcgcccat tttcagctct
    40441 aagtcttcac cagcttccgg cagtcggctg gcactacctg ccagcaacca gagcgcgcgt
    40501 ttgttggcac cacggaattt tgctcgcgcc accatctctt gtccggtata acagcctttc
    40561 ttaaagctga taccgcccag cgcctggaga ttggtcgcct gtgggataaa ctgcccgctg
    40621 ttggcggcat caatcaccgg gaaaccagct tcaatgttta atgccagcca ctgttggcta
    40681 ttgttcagtt ccgcttcacc gcgcagttta tcggtcagca tattagcagt agcttcatcg
    40741 gttacgatca ggaaacgttc tgccgggtgt tcaaaccata gcaaagtggt cgcgccttct
    40801 ttgactacct gtttttcttt cgaaggcagt tcgctaaaga gatttgccag cgcggcgcgc
    40861 gcctgaaaac cggcaacacc aagcagcaca cgctcgtcgt ctggagcgat ggtcacttta
    40921 gagaataccg catatttttt cagttcagtc agctgcggtt cacgcacgct gcgccgttca
    40981 atccatgcaa agccatcgcc gtcgcggaac agacgtaaat tgctccacat tttacctttg
    41041 gcgtcgcaat gggcggcgag caggtgctga tcttctgcca tctggctgac atctgctgtc
    41101 acctgaccct gcatatattt ttcgctgtcc gcgccagtaa tggtggcaag cgcccagtca
    41161 tcaagcgtca tcagcgtcag cggtaaacgg gcagaagccg taggctgacg tggaggaaaa
    41221 ggtgtaaaag ccataataga gtcctgagtt gcttaacggc gtattaatga ctaatggtaa
    41281 aagagctatg aggcaatgca agtgctttca ctacgcgatc tgtgccttct gcgaggcgac
    41341 tgccaaaaaa atgaagccct tgaattgttg tgaattaatt tcgcgatcgc gatggtgttt
    41401 actgatattc ccgtaaaaga agtcgaaaaa cacgttacaa tagccgggta ctggattttc
    41461 gtgagacagg aagaagatgg acattaacaa caaagcccgc attcattggg catgccgccg
    41521 tggtatgcgc gaactcgata tttcaatcat gccgtttttc gaacatgagt acgacagctt
    41581 aagcgatgac gaaaaacgca tctttattcg tctgctggaa tgtgacgatc cggacctgtt
    41641 taactggctg atgaatcacg gtaaaccggc cgatgcagaa ctggaaataa tggtccgact
    41701 catccagaca cggaaccggg aacgtggtcc tgtggcaatc tgatttgcgc gtctcctggc
    41761 gcgcacagtg gctttccttg ctgattcatg ggctggttgc cgctgttatt ttactcatgc
    41821 cctggccgct cagttacacc ccgttatgga tggtgttact ttcgctggtg gtatttgatt
    41881 gcgttcgcag ccagcggcgt attaatgctc gccaggggga aattcgcttg ttgatggacg
    41941 ggcgtttgcg ttggcaaggg caggagtgga gcatcgtcaa agcaccgtgg atgattaaga
    42001 gctgcatgat gctgcgttta cgttctgatg gcggtaaacg gcaacattta tggctggcag
    42061 ccgacagcat ggacgaagcc gaatggcggg atttacggcg gattttgttg caacaagaga
    42121 cgcaaagata aagatgacgg caaagcatat gcgctaagcc gtcagtgaga tcaggcgtaa
    42181 tgctctgcca tttcgttgag gatttgctcg caccagctct gaatacgctc gtcgctaagg
    42241 tcatactggt tagtttcatc cagcgccaga cccacgaaca gttgcccgtc agcaatcacc
    42301 ggtttcgggc tggtaaattc atatccttcc gttggccagt agccgacgaa cttcacgcct
    42361 ttggtcgaga gtttgtcatg cagcataccg agcgcatcga ggaaccactc gccgtatccc
    42421 agttgatcgc caagcccata cagcgcaaca attttacctt caaggttcag gtcgtcgagc
    42481 tgatcccaga cggcttccca gtcttcctgg atttcaccaa aatcccaggt cgggataccc
    42541 agaatgagca catcgtactg ctccattaat ttcggggagt cgtccttgag gttatgtaag
    42601 gtcaccagtt ctgggccgat aatatcgcgg attttttctg ccgccatttc ggtgtaacag
    42661 gtgctggaac cgtaaaaaag acccatattc ataacgtaag cttctcgatt ctgctgtggc
    42721 ttttgcagct agtgtatcag aatcgcttta gtcacggcac aataaggcat aatggatcaa
    42781 atgagtgacc ataaggggcg caagtgaaac aggatctggc acgcatcgag cagtttcttg
    42841 atgctctgtg gctggaaaaa aatctggctg aaaatacgtt gaacgcttac cgtcgcgatc
    42901 tgtcaatgat ggtggagtgg ttgcatcacc gcgggttgac gctggcgacg gcgcaaagtg
    42961 acgatttgca ggcattactg gcagaacggc tggagggcgg gtataaagcc accagctcag
    43021 cgcgtttgct gagtgcagtg cgccgattgt tccagtatct ttatcgcgaa aagtttcgtg
    43081 aagacgatcc cagtgcgcat ctcgcttcac cgaaattgcc ccagcgtttg ccaaaagatt
    43141 taagcgaagc gcaggtcgaa cgtttattac aggcgccatt aattgatctg ccactggagc
    43201 tacgcgataa agccatgctt gaagtgttgt atgctaccgg gctgcgtgtc tctgaactgg
    43261 tcgggctgac aatgagtgat atcagcctgc gtcagggcgt agtgcgggtc attggtaaag
    43321 gcaacaaaga gcgcctggtg ccgttagggg aagaggcggt ttactggctg gaaacctatc
    43381 tggaacatgg gcgtccgtgg ctgctgaatg gtgtgtcaat tgacgtgttg tttcccagcc
    43441 agcgtgcgca gcagatgacg cgacagacct tctggcaccg cattaaacat tatgccgtgc
    43501 tggcgggtat cgacagcgaa aagctgtcac cgcatgtgtt gcgtcacgct tttgccactc
    43561 atttattaaa tcatggtgcg gatttacgcg tggtgcagat gctactgggc cacagcgatc
    43621 tttccaccac gcaaatttat acgcatgtcg ctaccgagcg tctgcggcaa cttcatcaac
    43681 agcatcaccc gcgggcgtga tgtctgaaaa gaacgggaag atttatgaag aaaggtttta
    43741 tgttgtttac tttgttagcg gcgttttcag gctttgctca ggctgatgac gcggcaattc
    43801 aacaaacgtt agccaaaatg ggcatcaaaa gcagcgatat tcagcccgcg cccgtagctg
    43861 gcatgaagac agttctgact aacagcggtg tgttgtacat caccgatgat ggtaaacata
    43921 tcattcaggg gccaatgtat gacgttagtg gcacggctcc ggtcaatgtc accaataaga
    43981 tgctgttaaa gcagttgaat gcgcttgaaa aagagatgat cgtttataaa gcgccgcagg
    44041 aaaaacacgt catcaccgtg tttactgata ttacctgtgg ttactgccac aaactgcatg
    44101 agcaaatggc agactataac gcgctgggga tcaccgtgcg ttatcttgct ttcccgcgcc
    44161 aggggctgga cagcgatgca gagaaagaaa tgaaagctat ctggtgtgcg aaagataaaa
    44221 acaaagcgtt tgatgatgtg atggcaggta aaagcgtcgc accagccagc tgcgacgtgg
    44281 atattgccga ccattacgca cttggcgtcc agcttggcgt tagcggtact ccggcagttg
    44341 tgctgagcaa tggcacactt gttccgggtt accagccgcc gaaagacatg aaagaattcc
    44401 tcgacgaaca ccaaaaaatg accagcggta aataattcgc gtgaaacaac agatacaact
    44461 tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct gaattgcctc ccttgctgcg
    44521 ccgtttatac gccagccggg gagtacgcag tgcgcaagaa ctggaacgca gtgttaaagg
    44581 tatgttgccc tggcagcaac taagcggtgt cgaaaaggcc gttgagatcc tttacaacgc
    44641 ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc gacgccgacg gcgcgaccag
    44701 cacggctcta agcgtgctgg cgatgcgctc gcttggttgc agcaatatcg actacctggt
    44761 accaaaccgt ttcgaagacg gttacggctt aagcccggaa gtggtcgatc aggcccatgc
    44821 ccgtggcgcg cagttaattg tcacggtgga taacggtatt tcctcccatg cgggagttga
    44881 gcacgctcgc tcgttgggca tcccggttat tgttaccgat caccatttgc cgggcgacac
    44941 attacccgca gcggaagcga tcattaaccc taacttgcgc gactgtaatt tcccgtcgaa
    45001 atcactggca ggcgtgggtg tggcgtttta tctgatgctg gcgctgcgca cctttttgcg
    45061 cgatcagggc tggtttgatg agcgcggcat cgcaattcct aacctggcag aactgctgga
    45121 tctggtagcg ctggggacag tagcggacgt cgtgccgctg gacgctaata atcgcattct
    45181 gacctggcag gggatgagtc gcatccgtgc cggaaagtgc cgtccaggga ttaaagcgct
    45241 gctggaagtg gcaaatcgcg atgcacaaaa actagccgcc agcgatttag gttttgcgct
    45301 gggaccgcgt ctcaatgctg ccgggcgact ggatgatatg tccgttggtg tggcgctgtt
    45361 gctgtgcgac aacatcggcg aagcgcgcgt gctggcaaat gaactcgatg cgctaaacca
    45421 gacgcgaaaa gagatcgaac aaggaatgca ggttgaagcc ctgaccctgt gcgagaaact
    45481 ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg tatcaccccg aatggcatca
    45541 gggcgttgtc ggtattctgg cttcgcgtat caaagagcgt tttcaccgtc cggttatcgc
    45601 ctttgcgcca gcaggtgacg gtacgctgaa aggttccggt cgctccattc aggggctgca
    45661 tatgcgtgat gcgctggagc gattagacac actctacccc ggcatgatgc tcaagtttgg
    45721 cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat aaattcgaac tctttcaaca
    45781 acggtttggc gaactggtta ctgagtggct ggccccttcg ctattgcaag gcgaagtggt
    45841 gtcagacggt ccgttaagcc cggccgaaat gaccatggaa gtggcgcagc tgctgcgcga
    45901 tgctggcccg tgggggcaga tgttcccgga gccgctgttt gacggtcatt tccgtctgct
    45961 gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg gtcgaaccgg tcggcggcgg
    46021 tccactgctg gatggtattg cttttaatgt cgataccgct ctctggccgg ataacggcgt
    46081 gcgcgaagtg caactggctt ataagctcga tatcaacgag tttcgcggca accgcagcct
    46141 gcaaattatc atcgacaata tctggccaat ttagcgtcat cttctctata aaaaagagcg
    46201 tggattgggt acaatcccgc tcttatcacc gcattttgac tagctcaata aaagaaatca
    46261 gaccatgttt gaaattaatc cggtaaataa tcgcattcag gacctcacgg aacgctccga
    46321 cgttcttagg gggtatcttt gactacgacg ccaagaaaga gcgtctggaa gaagtaaacg
    46381 ccgagctgga acagccggat gtctggaacg aacccgaacg cgcacaggcg ctgggtaaag
    46441 agcgttcctc cctcgaagcc gttgtcgaca ccctcgacca aatgaaacag gggctggaag
    46501 atgtttctgg tctgctggaa ctggctgtag aagctgacga cgaagaaacc tttaacgaag
    46561 ccgttgctga actcgacgcc ctggaagaaa aactggcgca gcttgagttc cgccgtatgt
    46621 tctctggcga atatgacagc gccgactgtt acctcgatat tcaggcgggg tctggcggta
    46681 cggaagcaca ggactgggcg agcatgcttg agcgtatgta tctgcgctgg gcagaatcgc
    46741 gtggtttcaa aactgaaatc atcgaagagt cggaaggtga agtggcgggt attaaatccg
    46801 tgacgatcaa aatctccggc gattacgctt acggctggct gcgtacagaa actggcgttc
    46861 accgcctggt gcgtaaaagc ccgtttgact ccggcggtcg tcgccacacg tcgttcagct
    46921 ctgcgtttgt ttatccggaa gttgatgatg atattgatat cgaaatcaac ccggcggatc
    46981 tgcgcattga cgtttatcgc gcgtccggcg cgggcggtca gcacgttaac cgtaccgaat
    47041 ctgcggtgcg tattacccac atcccgaccg ggatcgtgac ccagtgccag aacgaccgtt
    47101 cccagcacaa gaacaaagat caggccatga agcagatgaa agcgaagctt tatgaactgg
    47161 agatgcagaa gaaaaatgcc gagaaacagg cgatggaaga taacaaatct gacatcggct
    47221 ggggcagcca gattcgttct tatgtccttg atgactcccg cattaaagat ctgcgcaccg
    47281 gggtagaaac ccgcaacacg caggccgtgc tggacggcag cctggatcaa tttatcgaag
    47341 caagtttgaa agcagggtta tgaggaacca acatgtctga acaacacgca cagggcgctg
    47401 acgcggtagt cgatcttaac aatgaactga aaacgcgtcg tgagaagctg gcgaacctgc
    47461 gtgagcaggg aattgccttc ccgaacgatt tccgtcgcga tcatacctct gaccaattgc
    47521 acgcagaatt cgacggtaaa gagaacgaag aactggaagc gctgaacatc gaagtcgccg
    47581 ttgctggccg catgatgacc cgccgtatta tgggtaaagc gtctttcgtt accctgcagg
    47641 atgtgggtgg ccgcattcag ctgtacgttg cccgtgacga tctgccggaa ggcgtttaca
    47701 acgagcagtt caaaaaatgg gacctcggcg acatcctcgg cgcgaaaggt aaactgttca
    47761 aaaccaaaac cggcgaactg tctatccact gtaccgagct gcgtctgctg accaaagcac
    47821 tgcgtccgct gccggataaa ttccacggct tgcaggatca ggaagcgcgc tatcgtcagc
    47881 gttatctgga tctcatctct aacgatgaat cccgtaacac ctttaaagta cgctcgcaga
    47941 tcctctctgg tattcgccag ttcatggtga accgcggctt tatggaagtt gaaacgccga
    48001 tgatgcaggt gatcccaggc ggtgccgctg cgcgtccgtt tatcacccac cataacgcgc
    48061 tggatctcga catgtacctg cgtatcgcgc cggaactgta cctcaagcgt ctggtggtcg
    48121 gtggcttcga gcgtgtattc gaaatcaacc gtaacttccg taacgaaggt atttccgtac
    48181 gtcataaccc agagttcacc atgatggaac tctacatggc ttacgcagat tacaaagatc
    48241 tgatcgagct gaccgaatcg ctgttccgta ctctggcaca ggatattctc ggtaagacgg
    48301 aagtgaccta cggcgacgtg acgctggact tcggtaaacc gttcgaaaaa ctgaccatgc
    48361 gtgaagcgat caagaaatat cgcccggaaa ccgacatggc ggatctggat aacttcgact
    48421 ctgcgaaagc gattgctgaa tctatcggta tccacgttga gaagagctgg ggtctgggcc
    48481 gtatcgttac cgagatcttc gaagaagtgg cagaagcaca tctgattcag ccgaccttca
    48541 ttactgaata tccggcagaa gtttctccgc tggcgcgtcg taacgacatt aacccggaaa
    48601 tcacagaccg ctttgagttc ttcatcggtg gtcgtgaaat cggtaacggc tttagcgagc
    48661 tgaatgacgc ggaagatcag gcgcaacgct tcctggatca ggttgccgcg aaagacgcag
    48721 gtgacgacga agcgatgttc tacgatgaag attacgttac cgcactggaa catggcttac
    48781 cgccgacagc aggtctgggg attggcatcg accgtatggt aatgctgttc accaacagcc
    48841 ataccatccg cgacgttatt ctgttcccgg cgatgcgtcc ggtgaaataa gcattacgtt
    48901 atgctcacaa ccccggcaaa tgtcggggtt ttttatttaa gctgggtaaa tgcagataat
    48961 cgtattctgg cttcgcgatt tgtcgcctgc atcaccatcc acggactgaa cgcccacggc
    49021 gtggcatcaa taccgcgtaa tacatcagct aaatcacacc attgataatc catcacttca
    49081 tcatcgttga tctgtaacgc actggtggtg cgtgcggcaa ataccggaca cacttcattt
    49141 tccacaatgc cactcggatc ggtggcgcgg tagcgaaagt caggatagat agattcagga
    49201 ggcgtaattt ccacgccaag ctcataacgg caacggcgga tcaccgcgtc ttcgctgctt
    49261 tctcccagtt gtgggtgccc acaaaccgag ttagtccaca cgccaggcca cgcttttttg
    49321 ctcagtgcgc ggcgggtaac taataattgc cccttggcat taaacagcca actggagaac
    49381 gcgagatgta agagggtgtc tgccgtgtgt gcggcatact tttccagcgt acccgtggga
    49441 actccctgtg cattcaataa aatgacgtgt tccgtttgca taatttctca catgtaattc
    49501 tgatcactca tcattataga taattgaaga gtgaaatgat taaccaagag agcgtttatc
    49561 gacaaccgcc cgttttgagc ggatgccgac aggtaaaaac catttaaaaa agtagcatca
    49621 tatatttgaa gcgactttca atggccattg caacacagtc agaaatgaca ggaatagaga
    49681 gagtaaaaag caacaacaga ctgaatatag cgcgcattaa aaaattcata ttattctcca
    49741 tgctcatttt tcttcagctt gcgcatcaga agccacatac gtactgtcct gacttttaaa
    49801 tctttatcag agacggattc cttcaccagg tcagcatgat gctgatagcc attaaagaag
    49861 acgttcagca caacggcgct aagggttgcg agcataatgc cgctatgtag cagcggttgc
    49921 agtacggccg gtaacttaga aaagaaatcg tgagagagcg tcggagtcat gccaacgccg
    49981 agactgatcg ccacaatata gaggttgtaa cggttggtgg tgtagttaca gcgcgacaga
    50041 attcgaatcc ctgtcgccag taccatgccg aacatcacta gaccagcgcc gcccagcaca
    50101 aattgcggaa tggaggctac cagcaccgcc atttttggca ccatgccaaa caggattaat
    50161 ataattcccg aagaaataca cacccagcgg ctatgaacgc gcatcacgct aaccaggcca
    50221 acgttttgag aaaatgacgt gtgggggaag ctgttaaacg tgccgcctat cattgtccct
    50281 acgccatcga cacgcagccc gcgaataata tcgtgtgaag agagtttacg accgactatt
    50341 tcacccagtg ccaggaacat ccccatcgac tcgataaaca cgatgattaa cacggcagtc
    50401 atggtcagaa tggaaacggg atcgaaaatc ggcataccaa acgacatcgg ggtgacaatc
    50461 gcaaaccacg aggcatcatg tagcccggat aaattgactt cattcatcat ccacgaaagt
    50521 aaaaagccaa atacaatccc cagtaatacg gcgacgttgg acataaatcc tttcgcatag
    50581 cgagtaatga gcaagataaa aattaagacg gcaaaggaga tacctaaata aacgggatta
    50641 ccatattgcg gattcccttt acctccagcg gcccagtcaa tacccacctg aatgatgcta
    50701 agcccaatag aagtaataac cacaccggta accagtggcg ggaataaagg catcaagcga
    50761 ccaataagtg gcgctaataa tgtggtgata aaacccgcgg cgatagtggc accaaatatc
    50821 cccagcaggc cgatatccgg gttcatccca atggctatca ttggtgttac agcagcaaag
    50881 gtcaccgaca taatcaccgg caggcggatc cccataaagc ggccgatacc gatacattgc
    50941 aataatgtga cgatcccgca gcaaaagaga tccgagctaa tgagcatcgc aatagcatct
    51001 tttgagaggc ccagtcggtc accaatcata agaggaacag cgactgcacc tgcgtacatg
    51061 acaaggacgt gttgcagacc gagtatgatc agctttcctg gtgataatat gcggtcaacc
    51121 tcatcagttg ggcggtcttg cccagaagat gagggaagtt gggaatctat ggcgctcatc
    51181 agtgtttctc ctttcgcccg ccagaataaa aagcaaccgt ttacgttaat gaaactgtct
    51241 aaacacatgc tcgtggagtg ccagtttttg cgaggaaggg gagttagaga caggaattgc
    51301 ggaggcaatc acataatcgt gcttaaacca cgaaaatgac aagagaacct gacaatggaa
    51361 ttatctgtaa ttttctcata ttgatatttc attaatcatt ctggcatgtc gcactctcgc
    51421 atttaatcgt ttttatctgg atagcgctct tttgatcggc aatgttgaaa taatgaaagc
    51481 ggcatagatt gaggggcaat aatttgaact cagtcgcaaa attataatta aaacggtcat
    51541 gctacctaat atatgcccgt ttattgcatc gcgaaatttg agaagacata actcattaaa
    51601 tgaaagggat gcaaatgaat aagtttatcg ctgctgaagc tgcggaatgt ataggctgtc
    51661 atgcttgtga aattgcctgt gcggtggcac acaatcaaga aaactggccg ctgagtcaca
    51721 gtgactttcg accgcgtatc cacgttgtag ggaaaggcca ggctgcgaat ccggtggcct
    51781 gccatcactg caacaatgcc ccttgtgtta cggcttgtcc ggttaatgct ctgactttcc
    51841 agtccgatag cgtacaactg gacgagcaaa aatgtattgg ctgtaaaaga tgcgcaatcg
    51901 cttgcccctt tggcgtcgtt gagatggtcg atacgattgc gcagaaatgc gacctttgta
    51961 accagcgcag ttccggcacg caagcctgta ttgaagtctg cccaacacag gcgttacgac
    52021 tgatggacga taaagggtta cagcggataa aggtggcccg ccagcgcaaa acggcagcag
    52081 gaaaagcgtc atcagacgct cagccatctc gcagtgcagc gttgctcccc gttaactcgc
    52141 gtaaaggcgc agataaaatt tcagcgagtg aacggaaaac ccactttggc gaaatctatt
    52201 gcgggctgga tccactgcaa gcgacttatg agagtgaccg ctgtgtttat tgtgccgaaa
    52261 aagctaactg caactggcat tgcccgctgc ataacgctat tccggattac atccgtctgg
    52321 tacaggaagg aaagattatt gaagcggcag aactttgcca ccagaccagt tccttaccag
    52381 aaatctgcgg cagggtatgc ccacaggacc gtctttgtga aggcgcatgt actttgaaag
    52441 atcactcagg cgcagtctct atcggtaatc tggaacgcta catcaccgat accgcgctgg
    52501 cgatgggctg gcgtcccgat gtcagcaaag ttgttccccg tatcgaaaaa gtggcggtga
    52561 ttggcgctgg gccagcaggg ttagggtgtg ctgatattct ggcgcgcgcg ggagttcagg
    52621 ttgatgtctt tgagcgccat ccagaaattg gcggtatgct gacttttggc attcctcctt
    52681 tcaaactcga taaaacggta ttaagccagc ggcgagagat attcaccgca atgggaatcg
    52741 actttcatct taactgtgaa attggccgcg atattacctt tagcgattta acttctgaat
    52801 atgatgcagt tttcatcggc gtggggactt acgggatgat gcgagcagat ctgccgcatg
    52861 aagatgcgcc cggtgtcatt caggctctac cgttcctgac tgcccatacc cgccagctca
    52921 tgggattgcc ggagtctgaa gagtatccgc tgacggacgt ggaaggtaag cgagtcgtgg
    52981 tattgggcgg tggcgatacg acaatggatt gtttgcggac ttccattcgc ctcaatgctg
    53041 ccagcgtgac ctgcgcgtat cgtcgtgatg aagtcagtat gccgggctcg cgcaaagagg
    53101 tggtcaatgc gcgcgaggaa ggtgtcgagt ttcagttcaa tgttcagccg caatatatcg
    53161 cttgtgacga agatgggcgt ttaactgcag tgggcctgat tcgtaccgcc atgggggagc
    53221 aggggccgga tggtcgccgt cgtcctcgtc cggtagcggg ttcagagttt gaattgcccg
    53281 ccgatgttct gattatggcc tttggtttcc aggcgcatgc catgccgtgg ttgcagggca
    53341 gcggaattaa actcgataaa tggggcctga ttcaaaccgg tgacgtcggg tatttaccta
    53401 cccagacgca tctgaaaaaa gtctttgctg gtggtgatgc agttcatggc gcggatctgg
    53461 ttgtcactgc catggccgca ggaaggcagg cggcgcgcga tatgttaact ctgtttgata
    53521 cgaaggcatc gtgatgaaat cgttaattat cgttaatccg gctgactgta ttggctgccg
    53581 tacttgtgaa gtagcctgtg tggtcgctca cccttcagaa caggagttga atgccgatgt
    53641 ctttttaccc cggctgaagg tacagcgact ggacatcatc agtgcgccgg tgatgtgcca
    53701 tcagtgtgaa aacgctcctt gtgttggcgc ttgccccgtg caggcgctga cgatgggcga
    53761 gcaggtggtg caggccaatt ctgcccgttg tattggctgt cagagctgcg tcagtgcgtg
    53821 tccgtttggg atgatcacca ttcagtcgtt gccgggggat acccggcaac aaatcgtgaa
    53881 atgcgatctc tgtgaacagc gggaagaggg gccagcctgc gttgaatctt gcccgacaca
    53941 ggcgctgcag ttgcttactg aaagagaact caggcgactc cgccagcagc gtattgttgc
    54001 cagcggtgag aatcctctct gaaagaaatc tccgcgctga aaggcgcgga gaatctatta
    54061 gattgcccaa ccacccgcgt aaaatgcgac cagtgcggca gtaatgatca ccgtaccaat
    54121 attcagcttt tgccattcgc gtgcaaagac gcggcctacg accagtgtca caaagcccag
    54181 cataataccg gtaacgatat tacaagtcag aacgatgaac acggcacaca ccaggccagc
    54241 catagcgtca ataaaatcat tgaaatccag cttcgagacg ttacttaaca tcagcaaacc
    54301 cacgtacatc agagcggggg cagtggcgta accagggatc aaaaatgaca gcggtgataa
    54361 aaacagaatt aacaggaata acgcccccac tacggttgcg gtcaaccctg ttttaccccc
    54421 ggcggcggtt cctgccgctg attcgatata aaccgctgcg ggcgctgcgc ccaccaggcc
    54481 ggagaatatt gaacttactg agtcactggt cagggctttg ccgccgttga tgatctggtt
    54541 gtctttatcc aacaaattcg cctgaccggc gacagcacgg atggtgccag tagcgtcgaa
    54601 cactgcggtc atcaccaatg ccagtacact cggaagtaca gttggctgga gtgcgcccat
    54661 aatatcgagg ctgaaaatca gagacttacc atcttcgcca gtcaggcttg gcatcgccac
    54721 cagaccgtgg tatttcaccg ctgggtcaaa gattaagccg atgatcgaaa ttgcaataat
    54781 caccaacaag atcccgccgg gtacgcgaca cttctccaga ccgaagatga ccgccagccc
    54841 cagtaagctc atcatcaccg ggaaggaggt aaacgcaccg agcgccactg gcaagccttc
    54901 aatcgggttt ttgataacca tacccacacc gttagcagca atcagcagca gaaacagccc
    54961 gataccgata cctgtaccgt gagcgatacc catcggcaaa ttacgtaaga tccaggtacg
    55021 cacaccggtt acggaaatgg cggtgaagat gacgcccatc agaaataccg cgcccagtgc
    55081 gacaggaacg ctaatttgtt gcccgagtac cagactgaat gcggtaaacg ccgtcaagga
    55141 aatcgcgcaa ccaatcgcca ttggcaaatt agcccataat cccatcagca acgagccgaa
    55201 gcccgcgacc agacaggtgg caacaaacac agctgcggga ggaaaacctg ctttgcccag
    55261 cattcccgga acgacgataa cggaataaac catggccaga aaggtcgtta agccagccag
    55321 tacttcctga cgaacggtac tgccacgagc ggtaatttta aaataattat ccagcgcgcc
    55381 ctgtggcttt ggtgcgtccg gtgtttgtag gatgtctcca gacataatga tgtcctcacg
    55441 aggatgttaa agttttatta ttagttgcgt tcgtacccca gacgaccatc aacgtaggtg
    55501 cggtagatcg aacggtcatc gcccaacgtc atcatcacga acaatttgtc gactaaagaa
    55561 acagagttgt catagcgcag ctgctgtagc ggagtggcgg tgggttccat caccacgaaa
    55621 tcagcctctt tgctaggtaa aaagttgcca atcaaatcgt caaggcccag agatttcgct
    55681 ccgccgagcg tggccaggta aaacgcttca tatgccgaga ggcgatagcc ttgtaattgc
    55741 aatactttgt aggcttcgtt cagcgtttgc agcatgttga aagtggttcc ggcaccgata
    55801 tccgttccca tgcccacttt aactttcttc tgccatgctt ttttcaagtt gaataagccg
    55861 ctgccgaggt aaaggttgga ggtcggacag aaagcaatgc tggatttggt ttcgctgaga
    55921 cgatcccact ctttttcttc gagatggacg cagtgagcaa agacacagtt tttaccggtc
    55981 agaccgtact gatggtaaac atccaga
//
LOCUS       DADLSE010000010        50019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLSE010000010 DADLSE010000000
VERSION     DADLSE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.010"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..318)
                     /locus_tag="JN692_000613"
     CDS             complement(<1..318)
                     /locus_tag="JN692_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7263008.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIH"
     gene            complement(335..595)
                     /locus_tag="JN692_000614"
     CDS             complement(335..595)
                     /locus_tag="JN692_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7263009.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(586..1856)
                     /locus_tag="JN692_000615"
                     /pseudo
     CDS             complement(586..1856)
                     /locus_tag="JN692_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN692_000616"
     CDS             complement(1934..2299)
                     /gene="queD"
                     /locus_tag="JN692_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7263010.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            2615..4414
                     /gene="cysJ"
                     /locus_tag="JN692_000617"
     CDS             2615..4414
                     /gene="cysJ"
                     /locus_tag="JN692_000617"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7263011.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            4414..6126
                     /gene="cysI"
                     /locus_tag="JN692_000618"
     CDS             4414..6126
                     /gene="cysI"
                     /locus_tag="JN692_000618"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7263012.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            6200..6934
                     /gene="cysH"
                     /locus_tag="JN692_000619"
     CDS             6200..6934
                     /gene="cysH"
                     /locus_tag="JN692_000619"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7263013.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            7198..7350
                     /locus_tag="JN692_000620"
     CDS             7198..7350
                     /locus_tag="JN692_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7263014.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            7544..8695
                     /locus_tag="JN692_000621"
     CDS             7544..8695
                     /locus_tag="JN692_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7263015.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN692_000622"
     CDS             complement(8781..9818)
                     /gene="iap"
                     /locus_tag="JN692_000622"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7263016.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTHDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10070..10978
                     /gene="cysD"
                     /locus_tag="JN692_000623"
     CDS             10070..10978
                     /gene="cysD"
                     /locus_tag="JN692_000623"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7263017.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVTL
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10980..12407
                     /gene="cysN"
                     /locus_tag="JN692_000624"
     CDS             10980..12407
                     /gene="cysN"
                     /locus_tag="JN692_000624"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7263018.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSLGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            12407..13012
                     /gene="cysC"
                     /locus_tag="JN692_000625"
     CDS             12407..13012
                     /gene="cysC"
                     /locus_tag="JN692_000625"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7263019.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13062..13385
                     /locus_tag="JN692_000626"
     CDS             13062..13385
                     /locus_tag="JN692_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7263020.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            13579..13890
                     /gene="ftsB"
                     /locus_tag="JN692_000627"
     CDS             13579..13890
                     /gene="ftsB"
                     /locus_tag="JN692_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7263021.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMIRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            13909..14619
                     /gene="ispD"
                     /locus_tag="JN692_000628"
     CDS             13909..14619
                     /gene="ispD"
                     /locus_tag="JN692_000628"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7263022.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSENSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            14619..15098
                     /gene="ispF"
                     /locus_tag="JN692_000629"
     CDS             14619..15098
                     /gene="ispF"
                     /locus_tag="JN692_000629"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7263023.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTFGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15095..16144
                     /gene="truD"
                     /locus_tag="JN692_000630"
     CDS             15095..16144
                     /gene="truD"
                     /locus_tag="JN692_000630"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7263024.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16125..16886
                     /gene="surE"
                     /locus_tag="JN692_000631"
     CDS             16125..16886
                     /gene="surE"
                     /locus_tag="JN692_000631"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7263025.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            16880..17506
                     /gene="pcm"
                     /locus_tag="JN692_000632"
     CDS             16880..17506
                     /gene="pcm"
                     /locus_tag="JN692_000632"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7263026.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            17646..18785
                     /gene="nlpD"
                     /locus_tag="JN692_000633"
     CDS             17646..18785
                     /gene="nlpD"
                     /locus_tag="JN692_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7263027.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            18848..19840
                     /gene="rpoS"
                     /locus_tag="JN692_000634"
     CDS             18848..19840
                     /gene="rpoS"
                     /locus_tag="JN692_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7263028.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSCMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(19960..20367)
                     /locus_tag="JN692_000635"
     CDS             complement(19960..20367)
                     /locus_tag="JN692_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7263029.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            20514..21107
                     /locus_tag="JN692_000636"
     CDS             20514..21107
                     /locus_tag="JN692_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7263030.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21107..22534
                     /locus_tag="JN692_000637"
     CDS             21107..22534
                     /locus_tag="JN692_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAY7263031.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            22545..22781
                     /locus_tag="JN692_000638"
     CDS             22545..22781
                     /locus_tag="JN692_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263032.1"
                     /translation="MICPRCADKQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(22821..24185)
                     /locus_tag="JN692_000639"
     CDS             complement(22821..24185)
                     /locus_tag="JN692_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HAY7263033.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSCGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGVVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTGDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(24280..25050)
                     /locus_tag="JN692_000640"
     CDS             complement(24280..25050)
                     /locus_tag="JN692_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HAY7263034.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDA"
     gene            complement(25055..25693)
                     /locus_tag="JN692_000641"
     CDS             complement(25055..25693)
                     /locus_tag="JN692_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7263035.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(25690..26952)
                     /locus_tag="JN692_000642"
     CDS             complement(25690..26952)
                     /locus_tag="JN692_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HAY7263036.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMHHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVAHCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(26949..27721)
                     /locus_tag="JN692_000643"
                     /pseudo
     CDS             complement(26949..27721)
                     /locus_tag="JN692_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            27887..28684
                     /locus_tag="JN692_000644"
     CDS             27887..28684
                     /locus_tag="JN692_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7263037.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN692_000645"
     CDS             complement(28735..29391)
                     /gene="pphB"
                     /locus_tag="JN692_000645"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7263038.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     CPVYRMQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN692_000646"
     CDS             complement(29497..32058)
                     /gene="mutS"
                     /locus_tag="JN692_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7263039.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNGPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            32134..32313
                     /locus_tag="JN692_000647"
     CDS             32134..32313
                     /locus_tag="JN692_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263040.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            32345..32698
                     /locus_tag="JN692_000648"
     CDS             32345..32698
                     /locus_tag="JN692_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7263041.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN692_000649"
                     /pseudo
     CDS             complement(32735..34803)
                     /gene="flhA"
                     /locus_tag="JN692_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN692_000650"
     CDS             complement(34877..35887)
                     /gene="hypE"
                     /locus_tag="JN692_000650"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7263042.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN692_000651"
     CDS             complement(35884..37005)
                     /gene="hypD"
                     /locus_tag="JN692_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7263043.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN692_000652"
     CDS             complement(37005..37277)
                     /gene="hypC"
                     /locus_tag="JN692_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7263044.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN692_000653"
     CDS             complement(37268..38140)
                     /gene="hypB"
                     /locus_tag="JN692_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7263045.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYRHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN692_000654"
     CDS             complement(38144..38494)
                     /gene="hypA"
                     /locus_tag="JN692_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7263046.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKCVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            38706..39167
                     /gene="hycA"
                     /locus_tag="JN692_000655"
     CDS             38706..39167
                     /gene="hycA"
                     /locus_tag="JN692_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7263047.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            39292..39903
                     /locus_tag="JN692_000656"
     CDS             39292..39903
                     /locus_tag="JN692_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7263048.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            39900..41724
                     /gene="hycC"
                     /locus_tag="JN692_000657"
                     /pseudo
     CDS             39900..41724
                     /gene="hycC"
                     /locus_tag="JN692_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            41727..42650
                     /gene="hycD"
                     /locus_tag="JN692_000658"
     CDS             41727..42650
                     /gene="hycD"
                     /locus_tag="JN692_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7263049.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            42668..44377
                     /locus_tag="JN692_000659"
     CDS             42668..44377
                     /locus_tag="JN692_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7263050.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFSNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDGMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            44387..44929
                     /gene="hycF"
                     /locus_tag="JN692_000660"
     CDS             44387..44929
                     /gene="hycF"
                     /locus_tag="JN692_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7263051.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            44929..45696
                     /gene="hycG"
                     /locus_tag="JN692_000661"
     CDS             44929..45696
                     /gene="hycG"
                     /locus_tag="JN692_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7263052.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            45693..46103
                     /locus_tag="JN692_000662"
     CDS             45693..46103
                     /locus_tag="JN692_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7263053.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46096..46566
                     /gene="hycI"
                     /locus_tag="JN692_000663"
     CDS             46096..46566
                     /gene="hycI"
                     /locus_tag="JN692_000663"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7263054.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            46609..47360
                     /locus_tag="JN692_000664"
                     /pseudo
     CDS             46609..47360
                     /locus_tag="JN692_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            47533..47844
                     /locus_tag="JN692_000665"
     CDS             47533..47844
                     /locus_tag="JN692_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7263055.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            47841..48260
                     /locus_tag="JN692_000666"
     CDS             47841..48260
                     /locus_tag="JN692_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7263056.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(48375..49799)
                     /locus_tag="JN692_000667"
     CDS             complement(48375..49799)
                     /locus_tag="JN692_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7263057.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(49808..>50019)
                     /locus_tag="JN692_000668"
     CDS             complement(49808..>50019)
                     /locus_tag="JN692_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7263058.1"
                     /translation="GLFTSVQFFDPANPMSIVWVFAVMALAVMLSFILTLLLGFEDIP
                     VEEAAAQARKHQSVQPTVAKEVSLN"
BASE COUNT        11805 a        12977 c        13456 g        11781 t
ORIGIN      
        1 atgaatacag aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat
       61 aattccatcc gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc
      121 gccttccagc aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac
      181 gatattactg atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc
      241 gatggccagt tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag
      301 caggtgtaag aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga
      361 atccggaagg cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg
      421 tgccacactc caggcagccg cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa
      481 aaagacctac cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag
      541 ccggaacaat gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag
      601 actcctgata acatcgcccg ccagatgcca caggccatga cgacgtaaat gacgccagaa
      661 taactggcgc agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc
      721 ctgcattaac gcaggccacg cccgatacca gccaggggtt gcaaaagcgc cggaacatgc
      781 tgataacgtt gtagaacatc ccatagcagg ctgcgctcta cgttgtgatg ataaagcgca
      841 aacagatttt gcggctcgcg gtgctggcag gcgcttatta gcgtttgtgc cgccgcctgt
      901 gcgccagtca gcgccatatc catgccgcgc acggaaattc cggtattgac gcaactgcgc
      961 aacgcatcgc ccaccagcag ccagccgtta ccggcgtatt gcaccggcat actgtgcaag
     1021 ccaccttctg gcaccagatg cgcaccatac tccagtgatt ccgtgttttt gataagcggg
     1081 cgcactgccg gatgcgcttt aaagcgagtc agcagctcgc ttgccggaac acgactttgc
     1141 gtaagggaag agagcgggca aacaatccct aacgagagcg tttgttgatt agtataaaga
     1201 aatgcgccgc cgggtaagtc atcacagatt ccgccgctga acagcaacgc tgcgccttcg
     1261 ttattctcca gatgaaaacg ttcttcaata gcggatgttt ccagcgacag cacttctttg
     1321 atccccaacg ccatcgcttc gccagcagga cgagtcacta acccgtgacg ttcagccagg
     1381 acgctgttgg caccttctgc cagcaccaca taacgggcgc ggagaatatc gtcaccacaa
     1441 ataacgccac agactctgcc gttttcttca tacagtgcat ccaccgtcgc tccggggatg
     1501 cattcgacac cttctttttc ggcttcggca accagccacg gatcgaaccg tgcacgtaat
     1561 acactccagg attcaccgcc gggctgtaag ctggaaaacg tcgttgcgcc atccggcgtt
     1621 aacagggaaa ggctttcgtg agtgatgcgt cgttcaagag gcgcggtcag atgaaattgc
     1681 gggaggagtt ccgcgagtgc atgggtatat aaccgcccgc cggaaagatt tttgctgccg
     1741 gggatttcag cgcgttcgag taacaaaacg gataaatccg ctcgcgcgca gcgtaacgcg
     1801 caagcggtgc ctgcaatacc ggcaccaata ataataatgt cgcagtcgtc ttccatgtca
     1861 tgccccagat atccagaaca caacacccta acatagcgtt actaaaggga aattgaccgc
     1921 cgacacactc ttatcattcg ccgcgataga tacaccctgc ggtgcaggtt tcttttacca
     1981 tcaccgcact taacagcggc acaacgggtt taacctgatc ccaaatccat tttgctaaaa
     2041 cttcgctggt tgggttttcc agacctggaa tatcattgag ataatggtga tcgaggcgct
     2101 cataagttgg tttaaacgcc gctttcagtt cagcgaaatc gataatccag ccagtatgcg
     2161 gatcgacttc cccggtaatt tccagtcgca ccataaagga atgcccgtgc aggcgaccac
     2221 atttatgccc ttccgggaca tgtggtaagc ggtgagcggc ttcgaaggtg aaatctttaa
     2281 ataacgtggt ggacatcata atttctctac aggacgataa aaccgccgta gagtaccgga
     2341 aaggctgttt tttcgcatta tctaaaacga ctttgttgcg caaaatcgct gatttatctt
     2401 aatgattggc taaattcatt tgtttttcat taggttggtt aatctatttt gttgttaaag
     2461 actattgcta aaacaggtta gtcgatttgg ttattagtta tcgctatccc gtctttaatc
     2521 cacaccgttc accccgttaa ccttaccttc tcttctgttt tatgggcgct gacagggcgc
     2581 agaaacagct ttgcttactg gaacataacg acgcatgacg acacaggtcc caccttccgc
     2641 gttgcttccg ttgaacccgg agcaactggc acgccttcag gcggccacga ccgatttaac
     2701 gcctacccag cttgcctggg tttctggcta tttctggggc gtgctcaacc agcagcctgt
     2761 tgcgcttgca gtgacgccag cgcccgccgc agaaatgccg ggtataacga ttatctctgc
     2821 ttcgcaaacc ggcaatgcgc gccgggttgc tgaagcatta cgcgatgatt tattagcagc
     2881 aaaactgaac gttaagctgg tgaacgcggg cgactataaa ttcaaacaaa tcgccagcga
     2941 aaaactgctc atcgtagtga cgtcaacgca aggggaaggg gaaccgccgg aagaagccgt
     3001 cgcgctgcat aagttcctgt tctccaaaaa agcgccaaag ctggaaaaca ccgcgtttgc
     3061 cgtgtttagc ctcggcgata gctcttatga atttttctgc cagtccggga aagattttga
     3121 cagcaagctg gcggaactgg gtggtgaacg cctgctcgac cgtgtcgatg ctgatgttga
     3181 ataccaggct gctgccagcg agtggcgcgc ccgcgtggtt gatgcgctta aatcgcgtgc
     3241 gcctgtcgcg gcaccttcgc aatccgtcgc tactggcgcg gtaaatgaaa tccacaccag
     3301 cccgtacagc aaagacgcgc cgctggtggc tagcctctct gttaaccaga aaattactgg
     3361 gcgtaactct gaaaaagacg ttcgccatat cgaaattgac ttaggcgact cgggcctgcg
     3421 ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac tggtgaaaga
     3481 acttgtcgaa ctgctgtggc tgacaggcga tgaacctgtc actgtcgagg gcaaaacgtt
     3541 gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg ccaacattgt
     3601 tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg gcgataaagc
     3661 gaagttacag cattacgccg cgacgacgcc gattgttgac atggtgcgtt tctccccggc
     3721 acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgaccccgc gtctctattc
     3781 cattgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg gtgtggtgcg
     3841 ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg ctgaccgcgt
     3901 ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc gcctgcccgc
     3961 taacctggaa accccggtga ttatgattgg cccaggcacc ggtattgcgc cgttccgcgc
     4021 ctttatgcag caacgcgccg ctgacgaagc gccaggtaaa aactggctgt tctttggcaa
     4081 tccgcatttt acggaagact tcctgtatca ggtggagtgg cagcgctacg tcaaagaggg
     4141 tgtgctgacg cggatcgatc tcgcctggtc gcgcgaccaa aaagaaaaag tttacgtaca
     4201 agataaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgacg gtgcccacat
     4261 ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac ttctggaagt
     4321 gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa gtgagctgcg
     4381 cgtagagcgt cgttatcagc gagatgtcta ctaatgagcg aaaaacatcc agggccttta
     4441 gtggtcgaag gaaaactgac agacgccgag cgcatgaagc ttgaaagcaa ctacctgcgc
     4501 ggcaccattg cggaagattt aaacgacggt ctgaccggcg gctttaaggg cgataacttc
     4561 ctgctgatcc gcttccacgg catgtatcag caggatgacc gcgacatccg cgccgaacgt
     4621 gctgaacaga agctggagcc gcgccacgcg atgctgttgc gctgccgtct gccgggtgga
     4681 gttatcacca ccaaacagtg gcaggcgatc gataaatttg ccggtgaaaa caccatctat
     4741 ggcagcattc gcctgaccaa ccgccagacg tttcagttcc acggcattct gaaaaagaac
     4801 gtcaaaccgg tgcaccagat gctgcactcg gtcggtcttg atgcgctggc gactgctaac
     4861 gacatgaacc gtaacgtact ctgcacctcg aacccttacg agtcgcagct gcacgcagaa
     4921 gcgtacgagt gggcgaagaa gatttctgaa catctgctgc cgcgtacccg cgcgtatgcg
     4981 gagatctggc tcgatcagga aaaagtcgcc accaccgatg aagaaccgat ccttggtcag
     5041 acctacctgc cgcgtaagtt caaaaccacg gtagtgatcc cgccgcagaa cgatattgat
     5101 ctgcacgcca acgacatgaa cttcgtggcg atcgcggaaa acggcaagct ggtgggcttc
     5161 aacctgctgg tgggcggtgg gctttctatc gaacacggta acaagaaaac ctacgcccgc
     5221 acggcgagtg agtttggcta tctgccgcta gagcatacgc tagcggtggc ggaagccgtc
     5281 gtgacaactc agcgtgactg gggtaaccga accgatcgta aaaatgccaa aaccaaatac
     5341 acactggagc gcgtgggggt tgagacgttt aaagcggaag tggagcgtcg cgcggggatc
     5401 aaatttgaac cgatccgccc gtatgaattt acagggcgcg gcgatcgtat tggctgggtt
     5461 aagggcattg atgataactg gcacctgacg ctgtttatcg aaaatggtcg catccttgat
     5521 tatccggggc gtcctctgaa aactggcctg ctggagatcg cgaagatcca caaaggtgat
     5581 ttccgcatta cggcgaacca gaatctgatc atcgccggtg tgccggaaag cgagaaagcg
     5641 aagatcgaga aaattgccaa agagagcggg ttaatgaatg ccgtcacgcc gcagcgtgaa
     5701 aactcgatgg cctgcgtgtc attcccgact tgcccgctgg cgatggcgga agcggagcgt
     5761 ttcctgccgt cttttatcga caacatcgat aatttaatgg cgaaacatgg tgtcagcgat
     5821 gagcatatcg tgatgcgtgt aacaggctgc ccgaacggtt gtggtcgcgc gatgctggcg
     5881 gaagtgggcc tggtgggtaa agcaccgggt cgctacaacc tgcatcttgg cggcaaccgc
     5941 attgggacac gtatcccacg gatgtataaa gaaaacatca ccgagccgga aatcctggcg
     6001 tcgcttgatg aactgatagg gcgctgggcg aaagagcgcg aagcgggtga aggcttcggc
     6061 gactttacgg tgcgtgcggg catcattcgc ccggtgctcg atccggcgcg cgatttgtgg
     6121 gattaaccat cagcccggtc ttgtaggcct gataagacgc gtgagcgtcg catcaggcaa
     6181 ggcaaacagt gaggaatcta tgtccaaact cgatctaaac gccctgaacg aactgccgaa
     6241 ggtagatcgc attctggcgc tggcagaaac taacgccgaa ctggaaaaac tggacgctga
     6301 aggccgcgtt gcctgggcgc tggataatct gcccggtgaa tatgtacttt cttccagctt
     6361 tggcattcag gcggcggtga gcctgcatct ggtgaatcag attcacccgg atattccggt
     6421 gatcctcacc gacaccggct atctgttccc ggaaacctac cgctttattg acgagttaac
     6481 ggacaaactc aagctcaacc tgaaagtgta ccgcgctacc gaaagcgccg cctggcagga
     6541 agcacgctac ggcaaactgt gggaacaggg cgttgaaggc attgaaaagt acaatgacat
     6601 caacaaagtc gaaccgatga accgggcgct gaaagaactg aatgcgcaaa cgtggtttgc
     6661 tggcctgcgg cgtgaacaat ccggcagccg tgccaattta ccggtgctgg caattcagcg
     6721 tggcgtattt aaagtgctgc cgattatcga ctgggataac cgaactattt atcagtacct
     6781 gcaaaaacat ggcctgaaat atcacccatt atgggacgaa ggatatttat cggtgggcga
     6841 tacccataca acccgtaaat gggaacccgg catggcggaa gaagaaacgc gtttctttgg
     6901 cttaaaaagg gaatgtggat tacacgaagg gtaaatattg ccggataaat taaacgcctc
     6961 tgtcagaaat gatgggggcg tttttatttt gcgagcagca ttccggcatt taaaggtgaa
     7021 catcgcaggg aaaattaaca ggtatagaat ggcgttgggt gcttgaggct gtctggcgtt
     7081 aagcattcgc gaggttccag atggacaaaa gccccaggcg atatttctat caacctgagg
     7141 ctcgcgttcg aacgtagcaa ttcggatgtt agtctcttct ttgcagggag gcaagacatg
     7201 ctgacaaaat atgcccttgt ggcaatcatc gtactgtgtt gtacagtact gggattcacg
     7261 ttgatggtag gtgactcgtt atgtgagttg agtatcagag aacgtggtat ggagtttaag
     7321 gcagttctcg cttacgaatc gaagaagtag ccaccacgcg ggggtaaaac cccgcgtact
     7381 gaattgtgat ggtttggtct cctgcaccct tttttaaaat ttatattttt tatgtgaata
     7441 tatagtctct atttattatt atgaatttca aatgaaaatc attaatttat tcataatgta
     7501 aatgttttat tggctgatat atatcacaga taatttttaa aatatgcgta aatatccttt
     7561 aagtttactg aaggataaaa atattgtgac tttctttgat ttctggggaa aaacccgacg
     7621 tggcgagaaa gagggtagcg acgactatca ccttctttgc tggcatttgc tggatgtggc
     7681 cgcaatgggc tatttaatgg ttaaaagaaa ttgcttcggg ctggctgatt attttcgtca
     7741 attagggatt tctgacaagg aacaggcagc tcaatttttc gcctggttgc tgtgctggca
     7801 cgatattgga aaatttgccc gctcttttca acaactttac ctgccccctg aattcaagat
     7861 tcaggaaggt gccagaaaaa attacgaaaa aatctctcat tcaacgctgg gctactggtt
     7921 gtggaatcat tatttaagtg aatgtcagga gttacttcct tcatcttcgc tctctccgcg
     7981 caaacttagg cgagtgatag aaatgtggat gcccgtgacg acagggcatc atggtcggcc
     8041 ccctgatcgt atggatgaat tggataattt tctgcctgaa gacaaagctg cagcacgaga
     8101 ttttctcctt gaaatcaagc cactgtttcc gcgaatagag atccccacat tctgggatga
     8161 tgacgagggc gttgaactta taaaacaact ttcctggtat atctctgcaa ccgtcgtact
     8221 tgcagactgg acagggtcgt caacgcgatt ttttccacgc gtcgcacagg caatggatat
     8281 tgaagattac tggcaaaagg ctttagttca ggctcaaaac gcattaaccg tctttccgcc
     8341 acaagcagaa accgcgcctt tcaccggaat taatacgctg tttcctttta ttgagaatac
     8401 gacaccgtta cagcaaaagg tgctggatct ggatatcaat cagccaggac cacagttgtt
     8461 tattctggaa gatgtgaccg gcgcaggtaa aacagaagcg gcgcttatcc tggcgcacag
     8521 gttgatggct gcgggtaacg ctcaggggct gttttttggc ctgccaacaa tggcaacggc
     8581 caatgccatg tacgatcgac tggtcaaaac ctggcttgct ttctattcgc cagaggtgtt
     8641 ccccgctcca gcggagataa accggcgcaa tacggtgcgg ttttcccgga agtaaaccgg
     8701 acgcactgga tgcgatgatg ggtatcactt agaattcccc gcccctgcgg tagaactccc
     8761 agctcccatt ttcaaaccca tcaagacgcc ttcgccagct ccttcaccaa cggtagcatt
     8821 atccgcataa cgtcacggca gcgacgttct attcttccag gaagtgcctt atcaatatgt
     8881 tgttgattat ccagtcttac gtcatgccag ctatttcccg ccgggaaggc aggtgttttt
     8941 gcgcgttgct gataaccatc cttatttcca agattccagt tagtcgcttc caccgaaagt
     9001 acagcaatgc ccgctttgtc gaatatttct gcgtcattac aacacccagt gcctttcgga
     9061 taatttttat tcaaacccgg attggtcgtt gcggctattc cgtgactgcg tgcaattgcc
     9121 agcgccctgt cgtgcgttaa tttccttact gcttcagggg tttttacacc gctgttgaaa
     9181 tacaatttat cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt
     9241 tcggtgtcac tcatccgctt gagtaaattc tcagcgccta attttccttc ctcttcgccg
     9301 ctggtcgcca caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccagt
     9361 tccagcatga cacctaaacc tgcggcgtta tcatctattc cttgtaacgt cagtccgccg
     9421 agattggcat ctgcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg
     9481 atgatctgct gcggcgcttt gccttcatga gcggcaatca ccgtacttcc tgtcacgtta
     9541 tgccagttct tgcgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata
     9601 tcactgcgat aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct
     9661 gctggagttc cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgggtatta
     9721 gcaaaatcgc caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc
     9781 gccagggcag cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc
     9841 aaattttttt accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc
     9901 taaacgcccg aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat
     9961 tccgttaagg aactactcat tctaattggt aatttcattc gttctcttac gctccctata
    10021 gtcgaaacat ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggatcaaaa
    10081 acgacttact cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc
    10141 ggcagaattc tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcacgct
    10201 gcatctggcg cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga
    10261 taccggctgg aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg
    10321 ctgcgagctg ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt
    10381 gcacggcagc gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa
    10441 caaatacggt tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc
    10501 taaagagcga atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg
    10561 cccggaactg tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt
    10621 cccgctctct aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga
    10681 cattgtgccg ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat
    10741 gatgattgat gacaaccgta tcgacctgca accgggcgaa gtgatcaaaa aacggatggt
    10801 gcgtttccgt acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac
    10861 actgccggaa atcatcgaag agatgctggt ttccaccacc agtgaacgtc agggtcgcgt
    10921 gattgaccgc gaccaggcgg gatccatgga gctgaaaaaa cgtcaggggt atttttaaga
    10981 tgaacaccgt acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg
    11041 cgcaacaaca taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa
    11101 gtaccctgat tggtcgtctg ctgcacgata cccgccaaat ctacgaagat cagctctcat
    11161 cgctgcataa cgacagtaag cgtcacggca cccagggcga aaagctggat ctggctctgc
    11221 tggtggacgg cctgcaagct gagcgcgaac agggcatcac tattgatgtg gcctaccgct
    11281 atttctctac cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca
    11341 ctcgcaatat ggcgactggc gcatcgacat gtgaactggc gatcttactg atcgatgccc
    11401 gtaaaggcgt gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca
    11461 aacatctggt cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa gagacgttca
    11521 cccgtattcg tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc
    11581 gctttgtgcc gctctccgca ctggaaggtg acaacgtggc ttcgcaaagt gaaagtatgc
    11641 cgtggtacag cggtccgaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg
    11701 tggatgccca gccaatgcgc ttcccggtgc agtacgttaa ccgcccaaat ctcgattttc
    11761 gtggctacgc cggaatgctg gcatccggtc gcgtggaagt cgggcaacgt gtaaaagtgc
    11821 tgccctctgg tgtggaatca aacgtcgcgc ggatcgtgac ttttgatggt gatcgcgaag
    11881 aagcctttgc cggagaagcg atcaccctgg tgctgacgga tgagatcgat atcagccgtg
    11941 gtgatctgct gctggcggca gacgaagcgt taccggcggt gcagagtgcg tcggtggatg
    12001 tggtatggat ggcggaacag ccgctttccc tgggccagag ttatgacatc aaaattgccg
    12061 gtaagaagac gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta
    12121 cccaacgcga agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg
    12181 acgagccact ggtgttagat cgctatcagc aaaatccggt taccggtggg ctgattttta
    12241 tcgatcgcct gagcaatgtg accgtgggtg ccggtatggt gcacgagcca gttagccagg
    12301 caacagctgc gtcatctgaa ttcagtgcat tcgaactgga attgaatgcc ctggttcgtc
    12361 gccactttcc gcactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga
    12421 cgaaaacgtc gtctggcata gtcatccggt cactgtgcaa caacgcgaac tacaccacgg
    12481 tcatcgtggt gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc
    12541 cggggcgctg gaggaggcgt tacataaact cggcgtcagt acgtatctgc tggatggcga
    12601 caatgttcgc cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa
    12661 tatccgtcgc gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac
    12721 cgcatttatc tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg
    12781 gcgctttatt gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa
    12841 aggcttatat aagaaagcgc gtgccggtga actgcgcaac tttacgggaa tagattccgt
    12901 ttacgaagcg cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt
    12961 ggtacagcaa ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac
    13021 cgggtttcca tgcccggttc gtcagagtaa caggatcaaa tatgcgcaat agccataaca
    13081 ttacactaac aaataacgac agccttactg aggatgaaga aactacatgg tcactgcctg
    13141 gtgccgtggt cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg
    13201 gctctaacac gctgttcttc tttatctaca cctggccttt ctttctggca ctgatgcctg
    13261 tcgcggtagt ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta
    13321 ttgttttcac cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg
    13381 gctaatttgt actttcttgt ctcctctgtt cataattcaa accgtaacta ataatgagat
    13441 tatgttctgc acgccctggg tatacgtaac aatggacaaa tgtggtacat ttgcccgcgt
    13501 tgtcgcggta tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc
    13561 cgtttttcag ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg
    13621 ctacagtatt cgctgtggtt cggtaagaac ggtatacatg actatacccg cgtcaatgat
    13681 gatgtggcgg cactgcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt
    13741 gccgaaattg acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa
    13801 ctcagcatga tcaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc
    13861 gcacagtctg cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact
    13921 catttggatg tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa
    13981 tgtcctaagc aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg
    14041 ctgctggcgc atccccgggt gacacgtgtc gtcattgcca taagtcctgg cgatagccgt
    14101 tttgcacaac ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag
    14161 cgtgccgatt ccgtgctggc aggtctgaaa gccgctggcg atgtgcagtg ggtattggtg
    14221 catgacgccg ctcgtccttg tttgcatcag gatgacctcg cgcgattgtt ggcgttgagc
    14281 gaaaacagcc gcacgggagg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc
    14341 gaaccgggca aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg
    14401 acgccgcaat ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa
    14461 ggcgctacca ttaccgacga agcctcggcg ctggaatatt gcggatttca tccgcagttg
    14521 gtcgaaggcc gcgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag
    14581 ttttacctca cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg
    14641 acgtacacgc ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg
    14701 aaaaaggatt gctggcgcat tctgatggcg acgtggcgct tcatgcgttg accgatgcat
    14761 tgcttggcgc ggcggcgctg ggggatatcg gcaaactgtt cccggatacc gatccggcat
    14821 ttaaaggtgc cgacagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg
    14881 gttatacctt tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc
    14941 acattccaca aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta
    15001 acgtgaaagc cactactacg gaaaaacttg gatttaccgg acgtggggaa gggattgcct
    15061 gtgaagcggt ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta
    15121 cctccacggt aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt
    15181 ggtggaagat ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct
    15241 caaaaacggc tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca
    15301 ttcccgtgaa gtcagcttcg ctgggcaaaa agacaaacat gctgtaacgg aacagtggtt
    15361 atgtgctcgc gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg
    15421 ccaggtgctg gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggtaa
    15481 cgcctttacc ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgat
    15541 cgatatttgc gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg
    15601 tagcaacttg cagggcgcgc tgcgctgggc gcaaaccaat actccggtgc gcgatcgcaa
    15661 taaacggagt ttctggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga
    15721 gcgcctcaaa aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg
    15781 acgtggtagc tggtttgtag caaccaccga agaactggcg gaattacagc gtcgcgtcaa
    15841 cgataaagag ctgatgataa ccgccgcatt gccgggcagt ggcgaatggg gaactcagcg
    15901 tgaagcgctg gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact
    15961 ggtgcgcgaa aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag
    16021 ctggaactgg tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt
    16081 tgcaaccagc gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga
    16141 gtaatgatga cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt
    16201 ttgctgacgt tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga
    16261 cactggaatc ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg
    16321 gaaccccgac cgattgtgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca
    16381 ttgttgtgtc cggaattaac gccgggccga atctggggga tgatgttatt tattccggta
    16441 cggtagccgc cgcgatggaa ggccgtcatt taggttttcc ggcgctggcc gtctcgcttg
    16501 acgggcataa acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt
    16561 gtaaagagcc gctgcgcacc ggacgtattc ttaatattaa cgttccggat ttacccttgg
    16621 atcaaatcaa aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga
    16681 tcccgcagca agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat
    16741 gtgatgccgg tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc
    16801 cgctgcatgt ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca
    16861 gcgtgggagt tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg
    16921 tgcgcaaggt attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa
    16981 attcgttgat gaagcgtttg aacaaaaagc ctgggacaat atcgccttgc cgataggtca
    17041 ggggcagaca atttcgcagc cgtatatggt ggcgcgaatg accgaattac tcgagctgac
    17101 gccgcagtcg cgggtgctgg aaattggcac tggttcggga tatcaaacgg caatcctggc
    17161 gcatcttgtc cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg
    17221 tcgccgcctg aaaaatcttg atttacataa tgtttcaacc cgtcatggcg atggatggca
    17281 aggttggcag gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat
    17341 tccaactgcg ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga
    17401 ggagcaccag tatttgaaac gggtgcgccg tcggggaggc gaatttatta tcgataccgt
    17461 ggaggccgtg cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata
    17521 cctggatttt tcctggttat tttgccgtag gtcagcgtat cgtgaacatc ttttccagtg
    17581 ttcagtaggg tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg
    17641 gataaatgag cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg
    17701 tttcgctatg gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg
    17761 ttaatggcaa tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa
    17821 tggggacgac gtctacagcg cagcaaccgc aaattcagcc ggtacagcag ccacaaattc
    17881 aggctactca acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga
    17941 tggaaaacgg acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata
    18001 gcggcagtac ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg
    18061 gcaacgattt ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg
    18121 ttggtcagac cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca
    18181 ttacccaggc cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg
    18241 ttgctgttgc gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa cagagtgcta
    18301 acaaaatgtt gccgaacaac aagccaactg cgaccacggt cacagcgcct gtaacggtac
    18361 caacagcaag cacaaccgag ccgactgtca gcagtacatc aaccagtacg cctatctcca
    18421 cctggcgctg gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca
    18481 acaaggggat tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc
    18541 gcgttgttta tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata
    18601 atgatgatta cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag
    18661 aagtgaaggc ggggcaaaaa atagcaacca tgggtagcac cggaaccagt tcaacacgct
    18721 tgcattttga aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc
    18781 gataaatcgg cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag
    18841 ccaccttatg agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga
    18901 tgagaacgga gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa
    18961 cgatttggcc gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac
    19021 tcagctttac cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta
    19081 ttttgcgcgt cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa
    19141 cttgcgtctg gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga
    19201 ccttatcgaa gagggcaact tggggctgat ccgcgcggta gagaagtttg acccggaacg
    19261 tggtttccgc ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat
    19321 tatgaaccaa acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta
    19381 cctgcgaacc gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat
    19441 cgcagagcaa ctggataagc cagttgatga cgtcagctgt atgcttcgtc ttaacgagcg
    19501 cattacctcg gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct
    19561 ggccgatgaa aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag
    19621 catcgtcaaa tggctgttcg agctgaacgc caaacagcgt gaagtactgg cacgtcgatt
    19681 cggtttgctg gggtacgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac
    19741 ccgtgaacgt gttcgccaga ttcaggttga aggcctgcgc cgtttgcgcg aaatcctgca
    19801 aacgcagggg ctgaatatcg aagcgctgtt ccgcgagtaa gtaagcatct gtcagaaagg
    19861 ccagtctcaa gcgaggctgg cctttttctt ttgtttggga agcttgtgtt tttgtcgcct
    19921 ggataagaca cgtcagcgtc ctacccggca atcagcattc tatgtgctca tcatcttgcg
    19981 caccaactgc ataaacagct cctgctcttc tccactcaaa cgccccaaaa attcctcatc
    20041 cacgctgtca ccaataggta tcgccgcagc aagtagcttt tctccttcgg cagtcagcca
    20101 gacaaagcgt cgccgcttgt cagcgacgtc atgttcacgt ctgactaagc cacgattttc
    20161 cattcttgcc aacatttctg ctagcgttgc tttggtgctg actgctgctt ctatcagtgc
    20221 cacctgttca ataccaggtt tatcagcaat ggcgcgcatc accgcgtact gtggtttggt
    20281 caggtcaggt aactcgtgct gccagcgagc cgtgtgctgc tgaaaaagct gtcgtaactg
    20341 atggaacgct ttatttcgta acgccatgta aactccgggc taattctctg tcgctatcat
    20401 agcggttttt tagtgacgaa gaagatgttg ttacttttca atccttgccg ctgtggaaat
    20461 gaaggagtac gttaataata acgttcgtat acgaacaatt aagagaataa gcaatgaaac
    20521 tgatcgtcgg gatgacaggg gctaccggtg cgcctcttgg tgtggcatta ctgcaagcgc
    20581 tgcgtgagat gccgaatgtc gagactcatc tggtgatgtc gaagtgggcg aaaaccacca
    20641 ttgaactgga aacgccttac agcgctcgcg atgttgctgc cctcgcagac ttcagccata
    20701 acccggcgga tcaggcggcg atcatctcat ccggttcttt tcgtaccgac ggcatgatcg
    20761 ttattccgtg cagtatgaaa acgctcgccg gtatccgcgc tggttacgcc gatggcctgg
    20821 tagggcgcgc ggcggacgtc gtgctcaaag aaggccgcaa actggtgctg gtgccgcgtg
    20881 aaatgccgct tagcaccatc catctcgaaa atatgctcgc actttcacgc atgggcgtgg
    20941 cgatggtgcc gccgatgcct gccttttata accatcccga aacggtagat gacattgtcc
    21001 accatgtggt agcccgcgtg ctggatcaat ttggcctcga acatccccac gccaggcgct
    21061 ggcaaggatt gccgcaggcc cggaattttt ctcaggagaa tgaataatgg catttgatga
    21121 tttacgcagc tttttacagg cgcttgatga ccacggccag ttactgaaaa tcagcgaaga
    21181 agtgaacgct gagccggatc tggcagcagc agctaacgcc accgggcgta tcggcgacgg
    21241 cgcgcccgcg ctgtggtttg ataatattcg cggctttacc gatgcccgcg tggcgatgaa
    21301 taccatcggt tcctggcaga accacgcgat ttcccttggc ctgccaccaa acaccccggt
    21361 caaaaagcag attgatgagt ttatccgccg ctgggataac ttcccgattg ccccggagcg
    21421 ccgcgccaat ccagcctggg cgcagaacac cgtcgatggc gaagagatca acctgttcga
    21481 tattctgccg ctgtttcgcc tgaatgatgg cgatggcggt ttctatctcg acaaagcgtg
    21541 cgtggtttcc cgcgatccgc tcgacccgga taacttcggc aagcagaacg tcggcatcta
    21601 ccgcatggaa gtgaagggca agcgtaagct cggcctgcaa ccggtgccga tgcacgatat
    21661 cgccctgcat ctgcataaag cagaagagcg cggtgaagat ctgccgattg cgatcacgct
    21721 cggtaacgat ccgatcatca cgctgatggg ggccacgccg ctgaaatacg atcagtctga
    21781 gtatgaaatg gcaggcgcgc tgcgtgaaag cccgtacccg atcgccaccg ccccgctgac
    21841 cggttttgat gtgccgtggg gttcagaagt gatcctcgaa ggggttatcg aaagccgtaa
    21901 acgcgaaatc gaagggccgt tcggtgagtt taccgggcac tactccggtg ggcgcaacat
    21961 gaccgtggtg cgcatcgata aagtctctta tcgcagcaaa ccgattttcg aatcactcta
    22021 tctcggtatg ccgtggaccg aaatcgacta cctgatgggg ccagccactt gcgtaccgct
    22081 gtatcaacaa ctgaaagccg agttcccgga agtgcaggcg gtaaatgcca tgtacaccca
    22141 cggcctgctg gcgattatct ccaccaaaaa acgctacggc ggctttgccc gcgcggtggg
    22201 cctgcgtgcg atgaccacgc cgcacggtct gggctacgta aagatggtga ttatggtcga
    22261 tgaagacgtt gacccgttca acctgccgca ggtgatgtgg gcgctctcct cgaaagtaaa
    22321 cccggcaggg gatttggtgc agttgccgaa tatgtccgtg ctggaactcg accctggctc
    22381 aagcccggcg gggatcaccg acaagctgat tatcgacgct accacgcctg tcgccccgga
    22441 caaccgtggt cactacagcc agccggtggt ggatttgccg gaaaccaaag cctgggctga
    22501 aaaactgacc actatgctgg ctgcacgtaa ataaggagaa caagatgatt tgtccacgtt
    22561 gtgccgataa acagattgaa gtgatggtga aatcgccggt gaaagatgtc tggacggtat
    22621 atcagtgcca gcattgcctt tatacctggc gcgataccga accgctacgc cgcaccagtc
    22681 gcgaacatta cccggaagcg ttccgcatga cgcagaaaga tattgatgat gcgccaatgg
    22741 tgccgagcat cccgccgctg ctggcggaag gtaagcgcta aataaaaagg tccgatgccc
    22801 atcggacctt tttattaagg tcaaattacc gcccatacgc accaggtaat taagaatccg
    22861 gtaaaaccga gaatggtcgt taacactgtc caggttttca gaccgtctgc taccgacaac
    22921 cccagatatt tggtcacaat ccagaaccct gagtcattaa tatgtgacgc cccaagccca
    22981 ccaaagcagg ctgccagcgt caccaatacg cactgaatcg gattcaatcc catcaccgct
    23041 tctgagagta acccgccggt tgtcagtatt gctacggttg ctgacccctg cgatgcacgc
    23101 agcgccagtg aaataataaa tgcggctggt aacagaggca ggtcaatcat ttgtaacatg
    23161 ttggcaaggg ctttgccgac gcccgattcc accagcactt tgccaaatac ccctccagca
    23221 ccagtaacca aaatcactac cgccgcagta ggaagcgctg agcccataat gtcgccggtg
    23281 tgttgtaagc tccagccgcg acgtaaagcc aataaccaga atgccagcac cagcgcaatc
    23341 attagagcta ccattggtga gccgatcagc tgtagcgtac caagcagggg atgcgaaggc
    23401 ggcatcagtg ttgcggaaac cgtacccgcc atgataatcg cgataggaat aacaattagc
    23461 gaggtgacca gcgcgacgcc cggtggattt attttatcgc ttaattttgt cgcgccttcc
    23521 tcactggccg gagccagttg catctgttcc agtacttcta ctgacatcgc atattggcgt
    23581 ttattgatta ttttcgctgc aaagtagcca acaaccccta cgggaataga aatcgcaata
    23641 ccgatgatgg ttagccagcc gatgtctgcg tggagtaacc ccgctgcggc gacagggcct
    23701 ggatgcggcg gtaccgccac gtgaacagtg agcatgatcc cagcgacagg caggccaaat
    23761 ttgagtggcg atattttggc taccttggca aaaccgtaaa tgattggcgc aagaataata
    23821 aagccgacat caaagaagac gggaataccg aggaagaacg ctgccagagt cagcgcagcg
    23881 atagttcgtt tgtcacctaa cttgcgactg aaataattag ccagtgactc tgcaccacaa
    23941 gagtgttcga tcatacgccc cagcatagcg cccagaccaa taataatagt gacggaacca
    24001 agcacaccgc ccatcccggc gatcatcact ttacccactt cgcccgccgg tatacccgcc
    24061 gcaagtgcga ctaacaggct gacgaggagc agagcaacga atggttgtac ctttgccttg
    24121 atgaccagca gcaacagcat gattacgcca gctaacgcaa tgcataacaa tgtaattgtg
    24181 gacatgggaa accctgtctg aaagttatag ttaacctacc ccatccgtag atggggggat
    24241 gtatgggtac gttgtaatta gggatttaac gaattagcgc taggcgtcaa accagccaag
    24301 cccttcttcg gtgaggccac gaggtttata ttcacaaccg atccagccct gatatcccac
    24361 ctcatcgaac aggcggaaca gccacggata gttgatttct ccatcgtccg gttcatgtcg
    24421 atcaggtagt ccggcaattt gtacgtgcgc atatttcccg gcgtagtcgc ggattaaatg
    24481 cgtcaggttg ccatctactt tttgcgcatg aaaagtatct agttgaataa acacgttatc
    24541 tcgcgcaacc tcttcaacaa tagccagtgc ctgatactgg ctggagaaga gataatgagg
    24601 cttaacgccg gggctgagtg cttcaactaa tattcgcttg ccgtgtggcg caaagcggtc
    24661 ggcagcgtag cggagattat cgataaatac tgcccggtac cgttcagcat cttcgccagc
    24721 gggcacgacg cctgccatca catggacttg ttcacaattg agcgccaatg catattccag
    24781 tgccaggtcg atgtctgcgt gtgcttcgtg ctcacgtccg ggaagggcgg ataatcccca
    24841 ttcccccgca ttaatatctc cgggagcggt attgaacagc gccagtgtca gatggttttg
    24901 ctccagttgc ttttggattt gcagggtgga gtagttatag ggaaacagaa attccacagc
    24961 atcgaacccg gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt cggtgaacat
    25021 catggataaa ttagctgcaa aacgaggcat tgcattaact ccttaattcc gcaatttcac
    25081 ctgcggtcag ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct
    25141 ccagctcttc catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat
    25201 gatttgccag taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc
    25261 gtttatcgcc cggtcggtaa tagggcacca gcgggacatt tcccatccgc atcaccacgt
    25321 atggtgtgaa cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcgccg
    25381 tcgaccatgt gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg
    25441 ccagatgaaa gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg
    25501 cgactttgga aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca
    25561 gtaaattccc gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc
    25621 gctgaaagaa tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg
    25681 cgaaatcgct catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa
    25741 gttgccagat ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc
    25801 gggggaaatg gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc
    25861 ggaggtctca ccgcctgcga caataaagcg tgtcacgcct tccgctgcta accgcgccgc
    25921 tagttgagaa aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg
    25981 aattgctgcc aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc
    26041 ctggcccaga acccactctg ccagttcgtg tgcataagcg gccagagttt cggttgagag
    26101 gcagtgtgcc acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctggcg
    26161 gttggtcatt tgagagcatg aaccggagag cactacgccg cgcccagcga gcggacgccc
    26221 tgcttcgcga gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag
    26281 accagaaccg cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg
    26341 gtgttcggtc agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag
    26401 ctcttgacga acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg
    26461 ccctgtggat tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg gatttaccgg
    26521 gtgatggtgc atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata
    26581 aaccgtacgt ccgttgaccg gcagggccgg agagaagacc gtaaacggcg tgtcgagagc
    26641 atccattaat gcatcggtaa ccgggccaat attacctttc gccgtactgt cgaaagtaga
    26701 gcagtatttg aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag
    26761 cgattgctgt gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga tcaccagtgc
    26821 gtcgattgct tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc
    26881 gttttccacc agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc
    26941 aatcttgatc atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg
    27001 ctatcgtctt ctttcccgta acccgcgtta ctggcgctgg tgaacatatt caatgctgtt
    27061 gaggccaatg gcagcgggaa gtgcagggct ttggctgtat cggtaaccag accaagatcc
    27121 ttaacaaaaa tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc
    27181 cggttttcga acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc
    27241 agcgggatcc ccgcacgggt gccagtcgtt caaaggcaat atcgctaccg gaggccatga
    27301 cagtcatttc accgttagcg gcttttactg caccaccaga aactggcgca tccagcattt
    27361 ccagatcgaa tccagccaga gcggtagcaa tttcttgcgc atcagcacta gcgatagtgg
    27421 aagaaaccat tactgccgta ccgggtttca gatgttgtgc aacgcctgtt tcaccaaaca
    27481 gcacctgttt aacctgggcc gcattgacca ccagcaccag cagagcgtcc agtttttcgg
    27541 caaacgtcgc ggcgttatca gaaaccccgc aagcacctgc ctctttcaac gtagcgcagg
    27601 cattgctgtt caggtctgcg ccccaggtag aaaaacctgc gcggacacat gacagtgctg
    27661 ctcccattcc cattgaccct aagccaacga taccgacatg aaactcagat cccgttttca
    27721 tctgctctta ttgttaattt aagtgatatt ttgtttgata ttgtgaatat gagcgctgaa
    27781 agataacgat atggtgagct gattcacata aattaacatt ttgtgttatt ttatgtgaac
    27841 taagcgttag ttgcgccgcg cacgtttcgc aggcaaatag cgtagaatgt cagcaggaca
    27901 aaggaaggag caaaagttga tacccgtaga gcgtcgccaa atcatccttg agatggtagc
    27961 tgaaaaaggc attgtcagta ttgctgaact aacggacaga attaatgtgt cacatatgac
    28021 cattcgtcgg gatttacaaa aactggagca gcagggagcc gttgtgctgg tgtccggagg
    28081 cgtccagtct ccgggacgcg tggcgcatga accttctcat caggtaaaaa ctgcgctggc
    28141 aatgacgcaa aaagcggcta ttggcaagct ggcggcaagt cttgttcagc cgggaagttg
    28201 tatctatctg gatgcgggaa cgaccacgtt agcgatagca cagcatctga ttcacatgga
    28261 gtcactgact gtggtcacaa acgatttcgt tattgcgaac tacttgctcg acaacagtaa
    28321 ttgcacaatt attcacactg gcggtgcagt gtgtcgggaa aaccgttcct gtgtcgggga
    28381 agccgctgcg accatgctgc gcagcctgat gattgatcag gcttttattt ctgcatcgtc
    28441 atggagtgtg cgggggattt ctacgccagc ggaagataaa gtcacggtga aacgggcgat
    28501 tgccagtgcc agccgccagc gagttttggt ctgtgatgcg acgaaatatg gtcaggtggc
    28561 gacatggctg gcgttaccat taagcgagtt tgatcagatt attacagacg acggtttgcc
    28621 ggagagtgcc agtcgcgcgc tggcgaagca ggatctctct ttgctggtag cgaaaaatga
    28681 ataatggcct gcaataacat ctggttactc atgcttcaca gaagaagcat gagactacct
    28741 tattttataa aatgacagcc gcccactttt cggcgatccg gtatcaatat aaatctggtt
    28801 agcgaacgtc tgaatgttat caaacatcat atgtccaaat ataaaataat cagcgccgtt
    28861 tatttgttgt aactcgccat taagcgattt ctgcatacga taaacaggac agagtaattc
    28921 gctctccgct atttctttgc caaagagata ttcatcaccc ggataatctg catgtgcgat
    28981 gacatatttt attatgtcgt tagtgatttc aataatatgt ggaaggtgat ggaatttcag
    29041 caacagatct attgcctctt gttgctctga atcatttaaa tcgaaaaacc agtcaccacc
    29101 gctggcaagc cacatattgc catcgccggt cgcgaacgca tccagcgcca ttgcttcgtg
    29161 gttgccttta acggagataa accagggctg gtttagcagg cgcaggacgt caagactctc
    29221 cggcccacga tcaatattat cgccgacaga aataagtaag tcggtttcgg ggaaaaaaga
    29281 gagttgatgt aagcgggatt gtaataactg atattcacca tgaatatcac caacgaccca
    29341 tatatggcga tagtgatggg cattgatttt ttgatagcgt gtagatggca tggttttacc
    29401 ctgtaaaata agctttccta ttatacaggg tatttttatt tgattcgtca gttgtcgtta
    29461 atattcccga tagcaaaaga ctatcgggaa ttgttattac accagactct tcaagcgata
    29521 aatccactcc agcgcctgac gcggagtcag tgaatccggg tcgagatttt ccagtgcttc
    29581 gaccgcaggc gaagtttctt ctggcactga cagcaaagac atctgcgtac catccacttg
    29641 cgtcgcggcg gcgttcggcg aaatgctttc cagctcacgc agtttttgcc gtgcgcgctt
    29701 aataacctct ttcggtacgc ccgccagagc agcaaccgcc agaccgtagc ttttgctcgc
    29761 cgcgccgtcc tgcacgctgt gcataaaggc gatagtgtcg ccgtgctcca gcgcatcgag
    29821 atgtacgttg gcgacgcctt ccattttctc cggtagctgg gtcagctcga aatagtgggt
    29881 ggcaaacagc gtcaatgcct taatcttatt cgccagattt tctgcgcacg cccacgccag
    29941 cgacagacca tcgtaggtgg acgttccacg cccgatctca tccattaaca ccagactgta
    30001 ttcggtggcg ttatgtaaaa tattggcggt ttcagtcatc tccaccataa aggttgagcg
    30061 cccggaagcc aggtcatctg ccgcgcctac gcgggtaaag atgcgatcga taggtccaat
    30121 ctcgactttt tgtgccggta catagctgcc gatgtaggcc atcagcgcaa tcagtgcggt
    30181 ctggcgcata taggtacttt taccgcccat gttcgggccg gtaataatca acatacggcg
    30241 ctgcggcgac agattcagcg ggttagcgat aaatggccca ttcagtactt gttcaactac
    30301 cggatggcga ccttcggtaa tgcgaatgcc cggtttatca atgaaggtcg ggcaggtgta
    30361 gttcagggta taggcccgtt ccgccaggtt aaccagcacg tcgagttccg ccagcgcgct
    30421 cgcgctctgt tgcaacgctt ccagatgcgg caacagcagg tcgaacagct cttcataaag
    30481 ctgtttttcc agtgccagtg ctttgccttt tgaggtgaga actttatctt cgtactcttt
    30541 tagctctgga atgatgtagc gctcggcgtt tttcagcgtc tggcgacgca tgtagttgat
    30601 gggtgccaga tggctttgcc cacggctgat ttgaatgtag tagccgtgca ccgcattaaa
    30661 gccaactttc agcgtgtcca ggccggtacg ttcacgctcg cggacttcca gacgctccag
    30721 ataatcggtc gcgccgtcag ccagcgcgcg ccactcatcc agctcttcgt tatagcccga
    30781 tgcgataaca ccaccgtcgc gtaccagcac cggcggtgtg tcgatgattg ctcgctccag
    30841 cagatcgcgc agctcggcaa actcgcccat cttctcacgt agcgcctgta ccggtgcact
    30901 atcgacagtt tctaactgcg cacgcagctc cggcagttgc tggaaagcgt ggcgcatacg
    30961 ggccagatcg cgtgggcgag cagttcgtaa agccagacgt gccagaatac gttccaggtc
    31021 gccgacctga cgcagtaccg gctgcaactc ggcggtgaaa tcctgcaatg cgccaatagt
    31081 ttgctggcgc tcaagcaaca cgcgggtatc gcgcactggc atatgcagcc agcgtttcag
    31141 catacgacta cccatcggcg tgacggtgca gtcgagcaca gaagccagcg tattttccgc
    31201 accgcccgcc aggttctgag taatttccag gttacgacgt gtcgcggcat ccataatgat
    31261 gctgtcctgc tcacgttcca tggtgataga acgaatatgc ggcagggtcg tgcgttgggt
    31321 atctttcgca tactgcaaca gacaaccggc agcacaaagt ccgcgcggcg cgttctcgac
    31381 gccaaaaccg accagatcgc gggtgccaaa ttgcagattc aactgctggc gcgcggtgtc
    31441 gatttcaaac tcccacagcg ggcgacggcg caggccgcga cggccttcaa tcagcgacat
    31501 ctcggcgaaa tcttctgcat acagcagttc cgccggatta gtgcgttgca gctctgccgc
    31561 catcgtttcg cggtcggccg gttcgctcag gcgaaaacgc ccggagctga tatccagcgt
    31621 cgcgtagccg aaacctttgc tgtcctgcca gatagccgcc agcaggttgt cctgacgctc
    31681 ctgtaacagg gcttcatcgc tgatggtgcc tggcgtaacg atacgcacaa ctttgcgctc
    31741 aaccggacct ttgctggtcg ccggatcgcc aatttgttcg cagatggcaa cggactctcc
    31801 ctgattcacc agtttggcga gatagttttc caccgcatgg tagggaatcc ccgccatcgg
    31861 gatcggctct cccgccgaag caccgcgttt ggtcagtgaa atatccagca gttgcgacgc
    31921 gcgttttgcg tcgtcataaa acagttcata aaaatcaccc atccggtaaa acagcaggat
    31981 ctcgggatgc tgggctttca gcttgagata ctgctgcatc atgggcgtat gggcgtcgaa
    32041 attttctatt gcactcatgg ggttatgtcc ggttccctga tattaaatgg ggtgtgatgg
    32101 tttttatttt cgttgtaatc acataaggcg cacatgatac cggagttaat catttcaggg
    32161 gaagaagcga acggcgagtt cttgatgctg gttccgggaa agatgctgtt ttgcagcttt
    32221 tgcgtgatgc ttatgaagca agtacgcaaa attgtatcga ttggcgaacc cttcttcttt
    32281 agagtcatag tgccctttaa gattaagatg taatataaat gcatcttaat gaggtggagg
    32341 cagcatgtcc ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa tgatcgatct
    32401 ttatcaagcg aaatgcccgc aggcgtcagc ggagccggag cattacgagg cgttgtttgt
    32461 ttacgcgcaa aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag cctgtaagca
    32521 gtgtccggtg cactgttacc agcctgcaaa gcgtgaagag atgaaacaga ttatgcgttg
    32581 ggccgggccg agaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc tcatcgatga
    32641 caagcgtccg gtgccagaac tgccggaaaa atatcgtccg aaaaaacctc gtgaatagta
    32701 tgccggacag gcagatctgt ccggcaattt gcagttaaat caatgccgat ttatcaattc
    32761 ccagccgctt catccgtgac agcagggtcg tgcgtttcag ccccagacgt tgcgcagcgc
    32821 ctttaggccc ggcaaccacg ctgttggttt ctttcagcac gcgcacaatc aactgatatt
    32881 catcttcgcc ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggc tccggtaaag
    32941 caatatctgg caatgacagc tgcagcacgt tgccgcgtgt tagcaatact gcgcgctcaa
    33001 tgacgttttc cagttcgcgt acgttacccg gccactccat gttgctcaag gtgcgcagcg
    33061 tctcggcagg aatgctgtcg atattgcgcc ccagacgacg ggcaattttg aaggtaaagg
    33121 ctttcgccag cagcggaata tcttccggac gctcgcgtag tggcggcagg tgaatcggga
    33181 atacgttcag gcggtaatag agatcgctac ggaactctcg gtcggcgacc atttttttca
    33241 gatcgcggtt agtcgcggca attaaacgta cgtccgtctg aatgattttg ttgctgccga
    33301 gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt cggctgtaac tccagtggca
    33361 tatcgcccac ttcgtcgagg aacagagagc ttttatccgc cagttcaaaa cgaccaatgc
    33421 gctgggcgct ggcaccggta aaagccccac gctcatgacc aaacagatcg ctttccagca
    33481 atccggcagg catcgccgcg cagttcattt tgaccatgcg gcgattatta cgcccactga
    33541 gattatggat cgcacgggca atcagctctt taccagtgcc agtttcaccg aggatcagca
    33601 cggtactgtc actttgcgcc accatttcaa cttgtttaag cacgctgtac atggcttcgc
    33661 tgcggccaat aatctcgcca aattcactat caacattgtt gagctgctcg gtcagggcga
    33721 ggttttcatc aaccagccgt tctttcagac gatggatttc ctgataggcg agggcgttat
    33781 cgacagcgat tgccacacgt tcggcaatct ggcgcagcaa attcagattg gtagtggtaa
    33841 acactttctc ttcgcattgc gccagtttca gcacgcccag catggtgtcg ccagacatca
    33901 gcggtaacag gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac atgcgttcat
    33961 agggggctaa atcgtcccgc tcgtggagat tgatcagcag catctcttta cttttgaaca
    34021 cgcgttcggt gagggttccg gcttcatcga cttcgctctg ttcgtgggcg ggatgttgtt
    34081 tatcaagata gtgagtggag tagatgttga gtttgttttt acggtggctg cgtaagacaa
    34141 tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg ctgaccagtt
    34201 cgtccatatc caggcgggaa agcaccgcgt tggtaatggc aaccaggatg cggaagttat
    34261 cgagctcccg gcataacaac tcatagtcga cattgttgac gacgcggctc tggatttgtt
    34321 cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg attgaactct ttttcgctcc
    34381 agggacgatc gtcataacga ataaattcac agccaccaaa aatatgccct tccgccgcca
    34441 gtggcatcag gcaatagtga ccaaatttgg ggtatagccc acctgcggcc aggtttcaca
    34501 aaattcttcg taactgcaat gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc
    34561 gtgtgccaga acagtttcgt cttcatattt aatgggggtg tctttttcac gcgacgcgta
    34621 ataagacgca cgttgagtct gcgcttgcca caacacaata gccgcgttgt cggcgagcgc
    34681 agaacgcttt accagttgcg aaagagcctc acacagcgag gccagatcgg gctgctgcaa
    34741 tagtgtccga gtgatgtcga acaacccttg ctgtccgaga tcactcatcg gtgtatatga
    34801 cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag
    34861 gagatttaga attttattag catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg
    34921 ttcgtttcac gccatacaga ccggcaagac gaacaccttt acgttccacc acttcaccaa
    34981 tcagcgccgc gtctttcccc agtggatggg aatgtaacgc tgccagcact tgctctgccg
    35041 cgttgcgttc aacggcgatc accagtttgc cttcgttggc aaagttcagg gcgtccagtc
    35101 ccagcaattc gcaaacgccg cgcacggcag gtttaaccgg cagcgctgat tcagaaattt
    35161 caataccaca accgcaggct gccgcgaact catgaaccac cgcgtttaca ccaccacggg
    35221 tggcatcacg cagcgctttc acgccgggaa tgtcacgcag cgtctgaata agcggcgtca
    35281 gcaccgcgca gtcgctgacc agttcgccat ccagccccag ctgctcacgc aggttaagga
    35341 tagtcgcccc gtggtcgccg agtgtaccgc taaccagcaa tacatcgcct gcggttagcg
    35401 tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca
    35461 gtttatctgc cgcgccgcgc tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg
    35521 ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt cagtgtctcc atcggcaatc
    35581 cttcttcgag gataaagcca caggagagat agcgcggaat agcgccactg accgcaacgt
    35641 cattcgcggt gccgcaaatc gccagcttgc cgatattacc gccaggaaag aacagcgggt
    35701 caatgacgta actgtcggtg gagaacgcca gacggtcgcc ttccgctacc agctgcgcca
    35761 gatcaagacg tgcctgatct tcctgctctg ccagccacgg gttggcaaag gcttccataa
    35821 acaggctgtt gattaattgc tgcatcgcct ggccgccgct gccgtgggcg agttggatat
    35881 tattcacgct tcactctcct gctgacgata ctgataccac gcggcgcacg ctccttcgga
    35941 ggagaccatc agtgcaccaa atgcggtttg aggattacag gtgttaccaa acagcgggca
    36001 ttgatgcggc ttacatttgc ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca
    36061 gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt
    36121 caggtgcacg ccagaagatt caatcacgcc taagccgcgc cattcgctgt cgccgttgac
    36181 acagaacaca tcggcaatcg cctgttgcgc cagcaggtta ccggcatccg gtaccacccg
    36241 acgatactga ttctctacct tgctgtgggc cgctattttc tgctccacca gcatgaccac
    36301 gccttgcagt agatcaaggg gttcgaaacc agcgaccacc agcggacgat gaaaatcgct
    36361 ggcgataaaa ttataggcat cagtgccgat aaccatacta acgtggcccg gcgcgaggaa
    36421 cgcgtcgata ccgttatccg gctgttccag caaactgcgc agcgtcggga taagcgtaat
    36481 gtgctggcag aagaagtaaa aattctgcac atcacgcgct ttcgcctgtt gcagagtgat
    36541 ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag aacaccactt tgcgggttgg
    36601 attctcctgc gccagtttca acgcatccat cggcgagtaa acgatgcgca catcggcacc
    36661 gcgtgctttt gcctgcaaca gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa
    36721 ggtacagaag atgacttccg gatggctggc aatctccacg caggtgtcga ttctacccat
    36781 cggcagtacg cacaccgggc aacccggacc gtggataaac tcaacgtttt caggcagtaa
    36841 ctggtcgagg ccgaatttaa aaatggcgtg ggtatgaccg ccacacactt ccataatccg
    36901 cagagggcgt tcggcggtgt aagagagatg tgaagcacgt tcgcgcagat gctcaattaa
    36961 ctgcatcacc tgttccggtg cgcgatattc atcaacaaaa cgcattattt ttcctcgcca
    37021 tacaacagcg cgccgacatc cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg
    37081 tcgcgtgctt cggcttcatt aattacgctc atggcaaagc caacgtgaac cagtacccac
    37141 tggcccacgc gcggctgacc gttttcatcg cagctgccga ctaacgttaa atcgacatcg
    37201 cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt caatggtgcg gatctggccg
    37261 ggaacgccta tgcacatcgc tgtgtctcca gccagttcag ccactggtcc atcccttcgc
    37321 cgctggtggc ggaaataagg atgatttcaa tttctggatt gacttcgcgg gcgcaggcga
    37381 tgcacttctc aacgtcaaag ttgagatacg gcaacaggtc aactttgttg agcagcatca
    37441 gcgaggcggc ggcaaacata tgccgatatt tcagcggttt gtcttcacct tcggtaacgg
    37501 aaagcaccgc tactttgtgt ttttcaccga gatcgaagct ggccgggcat acgaggttgc
    37561 caacgttttc gataaacaga ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg
    37621 caatcatctg tgcgtcaaga tggcagcctt taccggtgtt cacctgaatc gctggtgtac
    37681 cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg gtcgccttca ataaccgcgc
    37741 acggaacgct gtctttcagg cgcattaagg tttccgtcag cagggtggtt ttaccggaac
    37801 cagggctgga aaccaggttg agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc
    37861 gttcagccag acggttgttt ttgtccagca cgtcaatttc gacttccagc atccgacgct
    37921 ggctcatgcc cggtgcgtga gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt
    37981 cagtctggct gggggtaaat tcaggcgctt taatgccggt gattttcatc tgcgggcgtg
    38041 ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg gttatgttca tcaccctcga
    38101 tatacaggtt gccttcaccg caaccgcatg ttgtacacat cgctcactcc tggtctattt
    38161 ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg tagcatgtca ccatgacact
    38221 gtggacagcg gcggacgcgc tgggtcagta gcgtcacata ctgttggcat gtttcacacc
    38281 agcattcggc ctcttgttct tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc
    38341 aaaccagatc aaaacaaaag gcaagagagc tggtttcgac acaagaaaat gcgccaattt
    38401 tgagccagac cccagttacg cattttgcgc cgtgttttgc ggcctgctgt tcgatcaatt
    38461 ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc tcccattaac tattgccagc
    38521 tacaagcaat aattgcgcca gtgttgatta tccctgcggt gaataatgtc gatgatgtcg
    38581 aaatgacacg tcgacacggc gacgaaattc atctttagct taaaaatctc tttaataaca
    38641 ataaattaaa ggttggcaca aaaaatgctt aaagctggca tctctgttaa acgagtaacc
    38701 tgacaatgac tatttgggaa ataagcgaga aagccgatta catcgcacag cggcatcgtc
    38761 gtctacagga ccagtggcac atctactgca attcgctggt tcaggggatc acgttatcga
    38821 aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa agaactctgt ttcgtccttt
    38881 ttgaacattt tcgtatttac gtcaccctgg cggatggctt taacagccac accatcgagt
    38941 attacgtcga aacaaaagat ggcgaagaca aacagcggat tgcgcaggcg caactgagca
    39001 ttgacggcat gattgatggc aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact
    39061 atctcgaaaa aatcgctggc gtttacgaca gcttatacac cgccattgaa aacaatgtgc
    39121 cggtgaattt aagccaactg gtaaagggac aaagcccggc agcatgagct gaggctttgc
    39181 ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac
    39241 atttcatcgg catgttttcg tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt
    39301 tttgtaattg ccgactccac gctctgtatc ggctgccaca cctgtgaggc cgcctgttca
    39361 gagacgcatc gccagcacgg cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa
    39421 aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc
    39481 ccggttaacg ccatcacccg cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta
    39541 agctgcaaac tgtgcggcat cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt
    39601 ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc caccggcacc gcctgctccg
    39661 gcgcgtgtca gcacattgct tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt
    39721 gacctttgta gctttgatga acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc
    39781 ctgcatctgg tggataacac cgatatcgcc cgcgtcagca aacgtaagcg tgagctgacc
    39841 tttaacacgg actttggcga tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa
    39901 tgagcgcaat ttccctgatc aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg
    39961 catttctctt ttcttttcaa aaagcgctaa gtggctggat agccggaatt ggcggcgcgg
    40021 ttggtagtct gtatacggca gccgcgggct tcactgtact gactggcgcg gttggcgtga
    40081 gcggtgcgct gtcgctggta agctacgatg tgcaaatctc cccgcttaac gcgatttggc
    40141 tgattaccct cggcctttgc ggtctgtttg tcagcctcta taacattgac tggcatcgcc
    40201 acgcgcaggt gaagtgcaac ggcttgcaga tcaatatgtt gatggctgcc gccgtctgcg
    40261 ccgtcatcgc cagcaacctc ggcatgttcg tggtaatggc cgaagtcatg gccttgtgtg
    40321 cggtgttcct caccagcaac agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg
    40381 gcactctgct gctggtgatt gcttgctggc tgctgtggca gcgttatggc acgctggatc
    40441 tgcgcctgct ggatatgcgt atgcaacagc tgccgctcgg ttccgatatc tggctgctcg
    40501 gtgtgattgg ctttggcctg ctggctggga ttattccgct gcacggctgg gtgccgcagg
    40561 cacatgctaa cgcctctgcg ccagctgccg cgttgttttt tacggtagtc atgaaaattg
    40621 gcctgctggg catgttaacc ctgtcactgc tgggcggtaa tgcaccgctg tggtgggggc
    40681 gcgctgctgg tgctcggcat gatcaccgcg ttcgtcggtg gtctgtatgc gttgatggaa
    40741 cacaacatcc agcgcctgct ggcttaccac accctggaaa atatcggcat tatcctgctg
    40801 gggctgggcg ctggcgtaac gggtatcgcg ctcgaacaac cggcgctgat tgctcttggc
    40861 ctggtcggtg gtctgtacca tctgcttaac catagcctgt tcaaaagcgt actgttcctc
    40921 ggggcgggga gcgtctggtt ccgtaccggt catcgcgata tcgaaaaact cggtggtatt
    40981 ggcaagaaaa tgccggttat ctccatcgcc atgttagtcg ggttgatggc aatggctgcg
    41041 ctgccgccac tgaacgggtt tgccggggaa tgggttatct atcaatcctt cttcaaactg
    41101 agcaatagcg gcgcgtttgt tgcccgtctg ctggggccgc tgctcgctgt gggtctggca
    41161 attaccggtg cgctggcggt gatgtgtatg gcgaaagtct atggcgtcac gttcctcggc
    41221 gcgccgcgta ccaaagaagc cgaaaacgcc acctgtgcgc cgctcctgat gagcgtaagc
    41281 gtagtggcac tggcgatttg ctgcgtaatt ggcggtgttg ctgcgccgtg gctgctgccg
    41341 atgctctctg ctgctgtacc tctgcctctg gagcctgcta acaccaccgt ttctcaacca
    41401 atgatcacat tgctgctgat tgcctgcccg ctgctgccat tcatcattat ggcgattttc
    41461 aaaggcgacc gcctgccgtc acgttcccgc ggtgcggcct gggtgtgcgg ctacgaccat
    41521 gaaaaatcaa tggtgattac cgctcacggt tttgccatgc cggttaaaca ggcgtttgcg
    41581 ccagtgctga aactgcgcaa atggctgaat ccggtgtcgc tggtgccggg ctggcagtgc
    41641 gaaggtagtg cgttgctgtt ccgccggatg gcgctggttg aactggcggt actggtggtg
    41701 attattgttt cacgaggagc ctgagaatga gtgttttata tccgttaatt caggcgctgg
    41761 tgttatttgc cgttgcgccg ctgctctccg gtattacccg cgtggcgcgc gcccgtctgc
    41821 ataaccgtcg cgggccgggc gtgttgcagg agtatcgcga cattatcaaa ctgctgggcc
    41881 gccagagcgt tggcccggat gcctctggct gggtgttccg cctgacgccg tatgtgatgg
    41941 tgggcgtcat gctgactatc gctactgcgc tgccggtggt gaccgtcggt tctccgctgc
    42001 cgcaactggg cgatttgatc accttactgt atctcttcgc catcgcgcgt ttcttctttg
    42061 ccatttccgg tctggatacc ggtagcccgt ttaccgctat cggtgcgagc cgtgaagcga
    42121 tgcttggcgt gctggtcgaa ccgatgttgc tgcttggtct gtgggttgcc gcacaggttg
    42181 ccggttccac caacatcagc aacattaccg acaccgttta tcactggccg ctgagccaaa
    42241 gcatcccgct ggtgctggcg ctctgcgcct gtgcgttcgc cacttttatc gaaatgggca
    42301 aactgccgtt cgacctggcg gaagccgagc aggagttgca ggaaggtccg ctctccgaat
    42361 acagcggtag cggctttggc gtcatgaaat ggggtatcag cctgaaacag ctggtggtgt
    42421 tgcagatgtt cgtcggggtg tttattccgt ggggacaaat ggaaaccttc accgtcggtg
    42481 gactgctgct ggcgctggtg attgccatcg tcaaactggt ggtcggcgtc ctggttatcg
    42541 cgctgttcga aaacagcatg gcccgtctgc gtcttgatat tactccgcgc attacctggg
    42601 ctgggtttgg ctttgcattt ttagcgttcg tctccttgct ggcggcgtga ttaaagagag
    42661 tttgagcatg tctgaagaaa aattaggtca acattatctc gccgcgctga atgaggcatt
    42721 tccaggcgtc gtgctggacc acgcctggca gaccaaagat cagctgactg tcaccgtaaa
    42781 ggtgaactat ctgccggaag tggtggagtt tctctactac aagcagggtg gctggctgtc
    42841 ggtgctgttt agtaacgacg aacgcaaact gaatggtcat tacgccgttt attacgtgct
    42901 gtcgatggag aagggcacca agtgttgggt aacggttcgc gtcgaagttg acgccaacaa
    42961 accggagtat ccgtccgtga cgccgcgcgt tccggcggcg gtgtggggcg agcgcgaagt
    43021 gcgcgatatg tacggtttga ttccggttgg tctgccggat gaacgtcgtc tggtgctgcc
    43081 ggatgactgg ccggatgaac tttatccgct gcgtaaagac agcatggatt atcgtcagcg
    43141 tccggcaccg actaccgatg ctgaaaccta cgagttcatc aacgaactgg gcgacaagaa
    43201 aaacaacgtc gtgccgattg gtccgctgca cgtcacttct gacgaaccgg gtcacttccg
    43261 tctgttcgtc gatggcgaaa acattatcga cgccgactac cgtctgttct acgtccatcg
    43321 cggtatggaa aaactggcgg aaacccgcat gggttataac gaagtgacct tcctctctga
    43381 ccgtgtgtgc gggatctgcg gctttgccca cagcaccgcc tacaccacgt cggtggaaaa
    43441 cgcgatgggt attcaggtgc cagaacgcgc gcagatgatc cgcgccattc tgctggaggt
    43501 ggaacgcctg cactcgcatc tgctcaacct cggcctcgcc tgtcacttta ccggcttcga
    43561 ctccggcttt atgcagttct tccgcgtgcg tgaaacctcc atgaaaatgg cagagatcct
    43621 taccggtgcg cgtaaaacct acggcctgaa cctgatcggc gggattcgtc gcgatctgct
    43681 gaaagatggc atgatccaga cccgccagct ggcgcaacaa atgcgtcgtg aagtgcagga
    43741 gctggtggat gtgctgctga gtacgccgaa catggaacaa cgcaccgtcg gcattggtcg
    43801 tctggacccg gaaatcgccc gcgatttcag taacgttggc ccgatggttc gcgccagcgg
    43861 tctcgcccgc gatactcgcg ccgatcaccc gtttgtcggt tatggcctgc tgccaatgga
    43921 agtccacagc gagcagggct gcgacgttat ttcccgtctg aaagtgcgaa tcaacgaggt
    43981 ctataccgcg ctgaatatga tcgactacgg tctggataac ctgccgggcg gtccgctgat
    44041 ggtggaaggc tttacctaca ttccgcaccg tttcgcgctg ggctttgccg aagcgccgcg
    44101 cggcgacgat atccactgga gcatgaccgg cgacaaccag aagctgtacc gctggcgctg
    44161 ccgtgccgcg acctacgcga actggccgac cctgcgctac atgctgcgtg gcaataccgt
    44221 ttccgatgcg ccgctgatta tcggtagcct ggacccttgc tactcctgta ccgaccgcat
    44281 gaccgtagtc gatgtgcgta agaagaagag caaagtggtg ccgtacaaag aactcgagcg
    44341 ttacagcatt gagcgtaaaa actcgccgct gaaataagga atcgccatgt ttacctttat
    44401 caaaaaagtc atcaaaaccg gcacggcgac ctcgtcttat ccgctggagc cgattgcggt
    44461 tgataaaaac ttccgtggta agccagagca gaacccgcag cagtgcatcg gctgcgcggc
    44521 ttgcgtcaat gcctgcccgt caaacgcctt aacggttgaa actgacctcg ccacaggaga
    44581 gctggcgtgg gagtttaatc tcgggcgctg catcttctgt ggacgctgcg aagaagtttg
    44641 cccgacggcg gcgatcaaac tgtcgcaaga gtacgaactg gcggtgtgga agaaagaaga
    44701 cttcctgcaa cagtcccgct tcgcgctgtg caactgccgc gtctgcaatc gtcctttcgc
    44761 cgtacagaaa gagatcgact acgccattgc gctgcttaag cacaatggtg acagccgcgc
    44821 ggaaaaccac cgcgaaagct ttgagacttg cccggaatgt aagcgccaga aatgcctggt
    44881 gccgtccgac cgtattgaac tgactcgcca tatgaaagag gccatctgat gagcaattta
    44941 ttaggccccc gtgacgccaa cggcattccg gtcccgatga cggtggatga atccatcgcc
    45001 agcatgaagg cgtcgttact gaaaaaaatc aaacgttctg cctatgttta ccgcgtggac
    45061 tgcggcggct gcaatggctg cgaaatcgaa attttcgcca cgctttctcc gctgttcgat
    45121 gcagaacgct tcggcattaa agtcgttcct tcaccgcgtc atgcggatat tttactgttt
    45181 accggcgcgg tcacccgtgc aatgcgatcc cctgcgctgc gtgcgtggca gtccgcgccg
    45241 gacccgaaaa tctgtatctc atacggtgcc tgcggtaaca gcggcgggat cttccacgat
    45301 ctctactgcg tgtggggcgg tacggataaa attgtccctg tggatgttta tatccctggc
    45361 tgcccgccaa cgcctgccgc cacgctgtac ggctttgcaa tggcgctcgg cctgctggag
    45421 cagaaaattc acgcccgtgg gccgggtgaa ctggatgaac aaccggcgga gatcctgcat
    45481 ggcgatatgg tgcagccgct gcgcgtgaaa gtggatcgcg aagcacgtcg cttggcgggt
    45541 tatcgttacg gtcgtcagat tgccgatgat taccttacac agttagggca gggcgaagaa
    45601 caggttgcac gctggctgga agcggaaaac gatccgcgtc tgaacgagat tgtcagccat
    45661 ctgaatcatg ttgttgaaga ggcgcgtatc cgatgagtga aaaggtggtg ttcagtcaac
    45721 tgagccgtaa atttattgat gagaacgatg ccacgcccgc cgaggcgcag caggtggtct
    45781 attacagcct ggcgattggt caccaccttg gggttatcga ttgcctggaa gcggcgctca
    45841 cctgcccatg ggatgaatat ctggcatgga ttgccactct ggaggcgggc agcgaagccc
    45901 gccacaaaat ggaaggcgta ccgaaatatg gcgagatcgt catcgacatt aaccatgtgc
    45961 cgatgctggc caacgcattc gataaagccc gggcagcgca aacttcgcag cagcaggaat
    46021 ggagtacaat gctgttaagt atgctgcatg atattcatca ggaaaacgcc atctatttga
    46081 tggtgaggag actgcgtgac tgacgtttta ctctgtgttg gcaatagcat gatgggcgat
    46141 gatggcgctg gcccgctgct ggcggaaaag tgcgccgccg cgccgaaagg taactgggtg
    46201 gtgattgacg gcggtagtgc accggaaaac gacatcgtcg ccatccgtga actgcgcccg
    46261 acacgactgc tgattgtcga cgccacggat atggggctaa accccggcga aatccgcatc
    46321 atcgacccgg atgatatcgc cgagatgttt atgatgacta cccataacat gccgttgaat
    46381 taccttatcg accagttaaa agaagacatt ggtgaagtga ttttcctcgg cattcagccg
    46441 gatatcgtcg gcttttacta cccgatgacc cagccgatta aagatgcggt agaaaccgtt
    46501 tatcaacgac tggaaggctg ggaaggaaat ggtggatttg cgcagttagc ggtggaagaa
    46561 gaataattct ccatcaggga attcagggaa ttgaacatca gaacagggat gtgcgctttt
    46621 ttattgagct tagtgctgcc tgcacaggcc accagcttta ccgaatatct gccaatgagt
    46681 gacagcgaat acgcgcaaaa gcgggcgctg aaacctctcc tgaccatgcc ttatgatgcc
    46741 gagcaaacct ggcattttag gaaagtgggc gttgctggcg tgacgcttga gaagatgcca
    46801 aatgaagaag attactggca attaaccgct aaagaccgcg ccggtaaatc ctggactgtg
    46861 cctgtgggta tgctggcaaa catagcgggc aaagggcaac tctatcgcgc cgatcttgat
    46921 cgtaacggca ttcaggatct ggttatctgg ttatctggtt accaagtacc ggtaatggtc
    46981 tggctcctta tgcacattta attcttatga cgttcacgcg cgaaggccgt ccctgtgtct
    47041 ttgaacccag gggactctac accgcaagca aaactggcgt agatgatttg ctggatttgc
    47101 aaggcaatgg tcacacgcaa ttgctggata tgcagtttaa ttctggttac tggataacca
    47161 gtctgtatca ggtaaaagac gccaaatggc agcgtgttca cggctggttt gacaagttaa
    47221 gctatcccgc gctcacgcgt tttacttata cccccaaccg taaactcgtc ctcaaaccca
    47281 tcgccgggcg cgacccgcaa acggaagatc tggcgcagac gcagcgttgc ttgataaagg
    47341 gggatgtgct tgaggggtga accagaactg actttttgca gagtggctat gaggattttg
    47401 ggggtaatta tatcgcgcac aagtgtttac atattgtgaa cttggatttg atgagctata
    47461 gtttccgaac acataaggat agaactcgca gatgtggctg tttacttact ataaggaagt
    47521 taaaacttaa aaatgcacat catttctaaa gcgccatttg aagagtctgc gcgtaaatat
    47581 cctaacgacg ctctggctct tcaggcattg tatcgggtta ttaaggaaac ggatttttct
    47641 acgccagaag agatgcgaac ggcgtttccc aacctcgaca attttaaata tcgcaacaaa
    47701 tggtacgttc tggatgtagg tgggaacaac ctaagggtga ttgcctacat aaactttgtt
    47761 aataagcggt tctttgtgaa gcacataact aatcacgccg agtatgacaa actgacacgt
    47821 tactacagag agaacaaaga atgactgcta atgctgcacg cgctgttaaa gcaacaagag
    47881 aactggtcaa tgcagtcccc tttctgggag gtagtgactc agaagatgat tatcgtgaag
    47941 cactggaact ggtcgaatat ttgattgaag aagatgacac caatccactt atcgactttc
    48001 tggcgagccg cattgcagaa tatgaaaaca acaaccaaga gtttgcagag ttcgacaaag
    48061 ccgttgcggc aatgcctgtt ggcgtggcgt tacttcgcac gctgattgat caacataatc
    48121 tgacctatgc ggatttgaag aacgaaatcg gctctaaatc cctggtcagt cagattttgt
    48181 ctggacaacg atccctgacg atatcccaca ttaaggcact ttcagcccga tttggcgtta
    48241 agccggaatg gtttctttga tttttgacta atgccaaatg ctaaattgcc cgatgcgctg
    48301 cgcttatcgg gcctacatgt cgcacgaact atgtaggccg gataaggcgt tcacgccgca
    48361 tccggcactg ttacttactc taaatcttcc ccattactgg caatcacttt tttataccac
    48421 cagaacgatt tcttacgtgt gcgcgtcagc gtgccgttgc ctgcgtcgtc acggtctacg
    48481 tagacaaaac cgtagcgttt gctcatttca cccgtagagg cggaaactaa atcaatacag
    48541 ccccatgtgg tgtagcccat cagcggaatg ccatctgcaa tcgcttcgcc cattgcgcgg
    48601 atatgttcgc gtaaatagct gatgcgatag tcatcgttaa tctcgccatt ggcagcaagt
    48661 tcatcttttg cgccgaggcc gttttccacc agaaacagcg gcttctgata acgatcgtac
    48721 atcatattca tggtgatacg cagaccgagt ggatcaattc cccagcccca gtcgctcact
    48781 tgcagatacg ggttacgcag cgatttcacc acgttcgccg cactgctgtt gttggcgttc
    48841 atctccgccg aggcgcagcg cgaggcgtaa tagctgaaag agacaaaatc gacggtgttt
    48901 ttcaggatct catcatcgcc cagcgcttta tctatggtta cccctttttc gcgaaatacg
    48961 cgggcagagt aagccggata cgcgccgcgc gcctgcacat cgataaaaaa caggttttcc
    49021 cgatctttct ccagcgccgc ccagacatct tccggtttgc agctgtaagg gtagaagtta
    49081 ccgcccgcca gcatacagcc cacctgattt tgcgggttaa cctcatgggc gattttggtg
    49141 gctagcgcac tggcaaccag ctggtgatgc gcggcctgat atttcacctg atcctgattt
    49201 tcaccttctt caaacaccag acccgcgccg gagaacgggc tatgcaacat aatgttgatt
    49261 tcattgaagg ttagccagta tttcaccaga ccatcaaatg cttcaaagca ggttctggcg
    49321 tagcggctga aaaactccac cagcttgcgg ttacgccagg agccatattc ggtgaccaga
    49381 tgcatcggca catcgaagtg gcacaacgtg accagcggtt cgataccgta ctttttacac
    49441 tcttcaaaga caggacgata aaaagcaatg ccctgctgat tgggcgtgat ttcatcgccc
    49501 tgcggaaaga gacggctcca ggcaattgag gtacggaaaa ccttgaatcc catctctgcc
    49561 atcagggcga tatcttcttt ataacgatga taaaaatccg tcgcctcatg gctgggataa
    49621 aactcgtcat cgcgcaactg aaaacgtttt tccagcccca gtttcaccgc cattcgatgc
    49681 tcgccgtgtg ggatcatatc gacggtggtc agcccttttc cgccttcacg gaacgcacct
    49741 tcagactggt tggcggcaag cgcgccgccc cataaaaaac cttctggaaa tactgacatt
    49801 ttcatcctca attaagactt acttctttgg cgacggtcgg ttgtacgctc tgatgctttc
    49861 gcgcctgggc agccgcttcc tcaacaggaa tatcctcaaa gccgagcaac agtgtgagga
    49921 taaacgacag catcaccgcc agcgccatga ccgcgaacac ccagacgatg ctcattggat
    49981 tcgccggatc gaagaattgc acgctggtaa atagccccg
//
LOCUS       DADLSE010000011        49307 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLSE010000011 DADLSE010000000
VERSION     DADLSE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.011"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JN692_000669"
     CDS             complement(63..1193)
                     /gene="dnaJ"
                     /locus_tag="JN692_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7263059.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JN692_000670"
     CDS             complement(1282..3198)
                     /gene="dnaK"
                     /locus_tag="JN692_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7263060.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3575..3979
                     /locus_tag="JN692_000671"
     CDS             3575..3979
                     /locus_tag="JN692_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7263061.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4005..4718
                     /gene="msyB"
                     /locus_tag="JN692_000672"
     CDS             4005..4718
                     /gene="msyB"
                     /locus_tag="JN692_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7263062.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLHGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4867..5433
                     /gene="satP"
                     /locus_tag="JN692_000673"
     CDS             4867..5433
                     /gene="satP"
                     /locus_tag="JN692_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7263063.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNITGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JN692_000674"
     CDS             complement(5468..6055)
                     /gene="mog"
                     /locus_tag="JN692_000674"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7263064.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLASALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JN692_000675"
     CDS             complement(6170..7123)
                     /gene="tal"
                     /locus_tag="JN692_000675"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7263065.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7402..8832
                     /locus_tag="JN692_000676"
     CDS             7402..8832
                     /locus_tag="JN692_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7263066.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGFWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            8902..9678
                     /gene="yaaA"
                     /locus_tag="JN692_000677"
     CDS             8902..9678
                     /gene="yaaA"
                     /locus_tag="JN692_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7263067.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(9831..10127)
                     /locus_tag="JN692_000678"
     CDS             complement(9831..10127)
                     /locus_tag="JN692_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7263068.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="JN692_000679"
     CDS             complement(10341..11627)
                     /gene="thrC"
                     /locus_tag="JN692_000679"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7263069.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="JN692_000680"
     CDS             complement(11628..12560)
                     /gene="thrB"
                     /locus_tag="JN692_000680"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7263070.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="JN692_000681"
     CDS             complement(12562..15024)
                     /gene="thrA"
                     /locus_tag="JN692_000681"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7263071.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVFSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLDGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="JN692_000682"
     CDS             complement(15105..15170)
                     /gene="thrL"
                     /locus_tag="JN692_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7263072.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15384..16070)
                     /locus_tag="JN692_000683"
     CDS             complement(15384..16070)
                     /locus_tag="JN692_000683"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7263073.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16470..16610)
                     /locus_tag="JN692_000684"
     CDS             complement(16470..16610)
                     /locus_tag="JN692_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263074.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            16706..17422
                     /gene="arcA"
                     /locus_tag="JN692_000685"
     CDS             16706..17422
                     /gene="arcA"
                     /locus_tag="JN692_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7263075.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17481..18833)
                     /gene="creD"
                     /locus_tag="JN692_000686"
     CDS             complement(17481..18833)
                     /gene="creD"
                     /locus_tag="JN692_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7263076.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18891..20315)
                     /gene="creC"
                     /locus_tag="JN692_000687"
     CDS             complement(18891..20315)
                     /gene="creC"
                     /locus_tag="JN692_000687"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7263077.1"
                     /translation="MRIGMRLLRGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSVNKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPSEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20315..21004)
                     /gene="creB"
                     /locus_tag="JN692_000688"
     CDS             complement(20315..21004)
                     /gene="creB"
                     /locus_tag="JN692_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7263078.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21017..21490)
                     /gene="creA"
                     /locus_tag="JN692_000689"
     CDS             complement(21017..21490)
                     /gene="creA"
                     /locus_tag="JN692_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7263079.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21701..22570
                     /gene="robA"
                     /locus_tag="JN692_000690"
     CDS             21701..22570
                     /gene="robA"
                     /locus_tag="JN692_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7263080.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22567..23214)
                     /gene="gpmB"
                     /locus_tag="JN692_000691"
     CDS             complement(22567..23214)
                     /gene="gpmB"
                     /locus_tag="JN692_000691"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7263081.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23266..23787
                     /gene="yjjX"
                     /locus_tag="JN692_000692"
     CDS             23266..23787
                     /gene="yjjX"
                     /locus_tag="JN692_000692"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7263082.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(23872..24198)
                     /gene="trpR"
                     /locus_tag="JN692_000693"
     CDS             complement(23872..24198)
                     /gene="trpR"
                     /locus_tag="JN692_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7263083.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24288..26225)
                     /gene="sltY"
                     /locus_tag="JN692_000694"
     CDS             complement(24288..26225)
                     /gene="sltY"
                     /locus_tag="JN692_000694"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7263084.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGHSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26436..28103
                     /gene="ettA"
                     /locus_tag="JN692_000695"
     CDS             26436..28103
                     /gene="ettA"
                     /locus_tag="JN692_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7263085.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28411..29643)
                     /gene="nadR"
                     /locus_tag="JN692_000696"
     CDS             complement(28411..29643)
                     /gene="nadR"
                     /locus_tag="JN692_000696"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7263086.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(29664..31046)
                     /gene="radA"
                     /locus_tag="JN692_000697"
     CDS             complement(29664..31046)
                     /gene="radA"
                     /locus_tag="JN692_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7263087.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31095..32063)
                     /gene="serB"
                     /locus_tag="JN692_000698"
     CDS             complement(31095..32063)
                     /gene="serB"
                     /locus_tag="JN692_000698"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7263088.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LQMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32169..32813
                     /locus_tag="JN692_000699"
     CDS             32169..32813
                     /locus_tag="JN692_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7263089.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            32841..33857
                     /gene="lplA"
                     /locus_tag="JN692_000700"
                     /pseudo
     CDS             32841..33857
                     /gene="lplA"
                     /locus_tag="JN692_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            33889..34152
                     /locus_tag="JN692_000701"
     CDS             33889..34152
                     /locus_tag="JN692_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7263090.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(34313..35032)
                     /gene="deoD"
                     /locus_tag="JN692_000702"
     CDS             complement(34313..35032)
                     /gene="deoD"
                     /locus_tag="JN692_000702"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7263091.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35089..36312)
                     /gene="deoB"
                     /locus_tag="JN692_000703"
     CDS             complement(35089..36312)
                     /gene="deoB"
                     /locus_tag="JN692_000703"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7263092.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36364..37686)
                     /gene="deoA"
                     /locus_tag="JN692_000704"
     CDS             complement(36364..37686)
                     /gene="deoA"
                     /locus_tag="JN692_000704"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7263093.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(37813..38592)
                     /gene="deoC"
                     /locus_tag="JN692_000705"
     CDS             complement(37813..38592)
                     /gene="deoC"
                     /locus_tag="JN692_000705"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7263094.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            38850..40391
                     /locus_tag="JN692_000706"
     CDS             38850..40391
                     /locus_tag="JN692_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7263095.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDEIIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            40363..41226
                     /gene="yjjW"
                     /locus_tag="JN692_000707"
     CDS             40363..41226
                     /gene="yjjW"
                     /locus_tag="JN692_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7263096.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNGCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(41440..42222)
                     /locus_tag="JN692_000708"
     CDS             complement(41440..42222)
                     /locus_tag="JN692_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7263097.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(42219..43292)
                     /locus_tag="JN692_000709"
     CDS             complement(42219..43292)
                     /locus_tag="JN692_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7263098.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43414..43575)
                     /locus_tag="JN692_000710"
     CDS             complement(43414..43575)
                     /locus_tag="JN692_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7263099.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(43702..44307)
                     /gene="osmY"
                     /locus_tag="JN692_000711"
     CDS             complement(43702..44307)
                     /gene="osmY"
                     /locus_tag="JN692_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7263100.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(44700..46289)
                     /gene="prfC"
                     /locus_tag="JN692_000712"
     CDS             complement(44700..46289)
                     /gene="prfC"
                     /locus_tag="JN692_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7263101.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(46380..47057)
                     /gene="yjjG"
                     /locus_tag="JN692_000713"
     CDS             complement(46380..47057)
                     /gene="yjjG"
                     /locus_tag="JN692_000713"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7263102.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(47072..47518)
                     /gene="rimI"
                     /locus_tag="JN692_000714"
     CDS             complement(47072..47518)
                     /gene="rimI"
                     /locus_tag="JN692_000714"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7263103.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(47487..47900)
                     /gene="holD"
                     /locus_tag="JN692_000715"
     CDS             complement(47487..47900)
                     /gene="holD"
                     /locus_tag="JN692_000715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7263104.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48003..49034
                     /gene="rsmC"
                     /locus_tag="JN692_000716"
     CDS             48003..49034
                     /gene="rsmC"
                     /locus_tag="JN692_000716"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7263105.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11549 a        13442 c        12441 g        11875 t
ORIGIN      
        1 cgcatatctt caacgcaccc tatttttacc caggcctgcc cacgggcagg cttttgggga
       61 ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat caaagaagct ctttgagcgc
      121 gggctgttgt gctcgccggt tgggccaccg aagctttctt gcagctcttg cagcagctgt
      181 ttctgctttt cgttcaggcc taccggtgtt tcgacgacaa cgcggcacag caaatcaccc
      241 tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca tacggaatag cttaccggtc
      301 tgggtttcgc caggcacttt cagtttgacg cgaccatcaa gggtcggtac ttcgatttcg
      361 ccacccagcg ccgccatagc gaagttgatc gggacttcgc aatacaggtt gttgccttca
      421 cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt acagatcgcc tgccggtgcg
      481 ccatgctcgc ccgcttcacc ttcgcccgca agacggatgc ggtctccagt gtccacccct
      541 gccgggattt taacggacag cgttttgctg cgctcaacac gaccatgacc atgacatttg
      601 ttgcacggat ctttgatcag cgtaccgcgg ccctgacagt gtggacaggt ctgctgtaca
      661 gcgaagaatc cctggcgcat ctgcacctga ccagaaccat gacaggtcgg acaggtctgc
      721 ggctgtgtac ctggttttgc accgctaccg tggcaaacgt cacactcttc cagagtcgga
      781 atgcggatct ctttggtcac gccacgtaca gcttcttcga gggtgagctc catgttatag
      841 cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc cgccgccaaa aatatcgccg
      901 aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc caaaaccacc gccgcccatg
      961 ccaccttgct caaacgcagc atgaccatac tgatcgtatg ccgcacgttt ttgcgagtcg
     1021 gtcagaactt cataagcttc cttgatctct ttaaatttcg cctcggcctc tttgtcaccc
     1081 tggttacggt ccgggtggta tttcatggcc agccgtttgt aggcctttct gatttcacgc
     1141 tcttccgctg ttttggaaac gcctaaaatc tcgtaataat cttgcttagc catctttttt
     1201 aaattgcccc tagatgaatg cacgggcgga gaggaaattc cccttcgccc gtgtcagtat
     1261 aattacccgt ttatagggcg attatttttt gtctttgact tcttcaaatt cagcgtcgac
     1321 aacatcgtca tctttcgcgt tgtttgcaga agcatcagca ccggcagtct gctgctgggc
     1381 atgttgctgc tgggcgattt ccatcagttt ctgggaaacc tgtgccagtt cctgcatttt
     1441 cgcttcgata gcggctttgt cttcaccttt cagagcagtt tccagtgcag tcagcgcaga
     1501 ctcgatagca gttttgtcgt cagccggcag tttgtcgcct gcttcttcaa cctgcttacg
     1561 ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt accagctctt caaacttacg
     1621 gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc tggatttcat cttcgttcag
     1681 accagaagaa gccttgatgg tgatcttctg ctctttaccg ctgtttttat ctttcgcgga
     1741 aacgtgcagg ataccgtcag catcgatatc gaaggtaact tcgatctgcg gcatgccgcg
     1801 cggtgccggg ttgataccat ctaggttgaa ctgacccaga gatttgttat cagccgcacg
     1861 tttacgttca ccctgcagca catggatggt taccgcagac tggttgtctt cagcggtaga
     1921 gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc gcgatcagcg tcgtcatcac
     1981 accgcccatg gtttcgatac ccagagacag cggggtaacg tccagcagca gtacgtcttt
     2041 tacgtcacca gtcagaacac caccctgaac agcagcaccg attgctacag cttcgtccgg
     2101 gttaacgtct ttacgcggct ctttaccaaa gaactcagca actttcttct gaaccattgg
     2161 catacgagtc tgaccaccaa cgaggataac gtcgtcgata tcagatacgg acaggccagc
     2221 atcctgcagt gcaactttca gcggctcaat ggaacggttt accagatctt caaccaggct
     2281 ttccagtttc gcacgagtca ctttgatgtt catgtgtttc ggaccggtcg cgtctgcagt
     2341 gatgtatggc aggttaacgt cggtctgctg agcggaagac agttcgattt tcgctttttc
     2401 tgccgcttct ttcaggcgct gcattgccag cggatcgttg cgcaggtcaa tgccctgatc
     2461 tttcttgaat tcttcaacca gatagttgat cagacggctg tcgaagtctt caccacccag
     2521 gtgggtatca ccgttggttg ccagaacttc gaaggttttt tcgccgtcaa cttcgtcgat
     2581 ttcgataata gaaatatcga aagtaccacc ccccaggtca taaaccgcga tagtacggtt
     2641 gccagtgcct ttgtccagac cgtaagccag cgcagctgcg gtcggttcgt tgatgatacg
     2701 ttttacttcc agaccagcga tacggcctgc gtctttggtt gcctgacgct gagcatcgtt
     2761 aaagtatgcc ggtacggtga taacagcttc agttaccggt tcacccaggt aatcttcagc
     2821 ggttttcttc atttttttca gcacttcagc agaaatctgc ggcggtgcca ttttctggcc
     2881 tttaacttcg acccatgcgt cgccgttatc agcagcaata attttgaacg gcatgatgga
     2941 aacatcacgc tgtacttctt cgtcctggaa gcggcggcca atcaggcgtt taatcgcaaa
     3001 cagggtgttt tgcgggttcg tcactgcctg acgtttagcc ggctgaccaa ccagagtttc
     3061 accatcctgg gtataggcaa tgatagaagg cgtggtgcga tcgccttcgg cgttctccag
     3121 cacgcgagga gtggtgccat ccataatcgc tacacaagag ttggtagtac ccaggtcgat
     3181 accaattatt ttacccatct aaacgtctcc actatatatt cggtcatcat gtggttgtga
     3241 gtctgtaata ggggcgaaac gtctggtttc aactgcccaa tttcattttt ttgcagactc
     3301 actcactgcg gttgactact aaatggggtc gtaacccacg tcatcaaggg ggagatgcaa
     3361 aaattttttg tgcttttgcg cagaaatttc gcgatatggc aggacaagac gtaaaattga
     3421 cagcaatttt attcatataa agaatgaatc gcagccaagg cattcatcaa aaaattgcaa
     3481 taaaaagaaa agattacgtg cctgaatctt ctctttatca gcagtaaact agtgggtatt
     3541 catcccccta cctcttccca ctaagagaat ccttatgaaa tccgttttta cgatttccgc
     3601 cagcctggcg attagcctga tgctgtgctg cacggcgcag gcaaacgacc ataaactcct
     3661 cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg gcgctgaaac ttcctgtttg
     3721 tcgcattgtg aaacgcatac aactctctgc cgaccatggc gatttacagt taagcggtgc
     3781 atcggtttat ttcaaagccg cccgtagcgc cagtcagagc ctgaatattc cttcagaaat
     3841 aaaagaaggg caaaccactg actggatcaa cattaacagc gataacgaca ataaacgctg
     3901 cgtctcaaaa atcacctttt ccggtcatac ggtgaactca tcggatatgg ccacgctgaa
     3961 aattatcggc gacgactaac gccagaatat agaagccaca aaaaatgaat gttaattacc
     4021 tgaatgattc agatctggat tttcttcagc attgtagtga ggaacagttg gcaaatttcg
     4081 cccgattgct cacccataat gaaaaaggca aaactcgcct ctccagcgta ctgatgcgca
     4141 acgaactgtt taaatcgatg gaagggcatc ccgagcaaca tcgccgcaac tggcagctga
     4201 ttgccggaga attacagcat tttggtggcg atagtatcgc caacaaactg cgcggacacg
     4261 gtaaattgta tcgggccatt ttgctcgatg tctcaaagcg attgaagctg aaagccgaca
     4321 aagagatgtc tacgtttgaa attgagcaac agttactgga acaatttctg cgtaatacct
     4381 ggaagaaaat ggacgaggaa cataagcagg agtttctgca cgcggtcgat gccagggtga
     4441 atgagctgga agagctgctg ccgctgctga tgaaagacaa attattggca aaaggtgtgt
     4501 cgcatttgct ttccagccaa ctgacccgca ttttacgcac ccacgcagca atgagcgtac
     4561 ttgggcatgg tttgctgcac ggcgcggggc tgggaggccc tgtgggcgca gcattaaatg
     4621 gggttaaagc ggtcagcggc agcgcctatc gcgtgacgat tccagccgta ctgcaaatcg
     4681 cctgcctgcg ccggatggtt agcgccactc aggtctgata ccggaaactg gggaaaattt
     4741 tcattttccc ctcaaaagat cgtagacact gccccactgg ctgattatta tgccgcgccc
     4801 tgaaaacact acagttattc agggaaatta tttcaccatt cattcgatga tgatttttga
     4861 ggaattatgg gcaacactaa gttggctaat ccggcaccgc tgggcctgat gggcttcggc
     4921 atgaccacca ttctgcttaa cctgcacaac gtgggttatt tcgctctgga cggtattatt
     4981 cttgccatgg gcattttcta cggcggcatc gcgcaaattt ttgctggtct gctggagtac
     5041 aaaaaaggca acactttcgg tttaaccgca ttcacctctt acggttcttt ctggctgacg
     5101 ctggttgcga ttctgctgat gccgaaactg ggtctgaccg atgcgccaaa tgcacagttc
     5161 cttggtgtct acctgggtct gtggggcgta tttacgctgt ttatgttctt cggcactctg
     5221 aaaggcgcac gcgtcctgca attcgttttc tttagcctga ccgtgctgtt tgccctgctg
     5281 gcgatcggta acattaccgg taatgccgcg atcatccact ttgccggctg gattggtctg
     5341 atctgcggtg ccagcgcaat ctatctggcg atgggtgaag tgctgaacga gcagtttggt
     5401 cgcaccgttc tgccgattgg tgaatcccac taattgtctt gagatccccc cgctcggggg
     5461 gattttttta ttcgctaacg tcgcgtcttg cactcttcgg tctgaatgca gcaaccactt
     5521 ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat gcagtacggt acgctggcaa
     5581 aaataccatg caccacaacg ttaccctcag cgtccttcac accttccagc gtctctttaa
     5641 tagacttcgg ctgaccgggt aagttaagga tcagcgcctg tttgcgaatc acgcccacct
     5701 gacgcgaaag gatcgcagtt ggtacaaaat gcaggctgat ctggcgcatc tgttcaccaa
     5761 agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc gggcgttacg tcacgacgcg
     5821 ccgggccagt tccgcccgtg gtgagcacca gatggcaact catttcatcc accagctcac
     5881 acaacgtttg ctcgatgatc gcctgctcat cggggattaa gcgggtttcc agttcaaacg
     5941 gcgtggttag cgccgatgcc agccattctt ccagcgctgg aatgccttta tcctgataaa
     6001 cgccgctgga tgcgcgatca gagatggaaa ctaagccaat acgtaaagta ttcatcaaga
     6061 tttttccgtt taaacagtct cgttaaacag aatgatacac tgcgaaggga gtgacagaca
     6121 ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc taagaatgat tacagcagat
     6181 cgccgatcat tttttccagt ttttcctggt caatagcaaa cttacggata ccttccgcca
     6241 gtttatctac tgccattgga tcctggttgt gctgccacag gaactcggac tcagtgatac
     6301 gcgccggacg cgctttcact tcgccggtgt aagacagttt acgttcgata gccccttcgc
     6361 tctccgccag ctctttcagc agtgccggtg cgatggtcag acggtcacag cctgccagtt
     6421 ccagaatttc gccgatgtta cggaagcttg cgcccataac cacggtttca taaccgtgct
     6481 ctttgtagta ctggtagatt tcagatacag aaaccacgcc cggatcttct gccggagcgt
     6541 actctttctt atcggtattc gctttgtacc agtcaagaat acggccaaca aacggcgaga
     6601 tcaggaacac gcccgcctcc gcacaagcac gagcctgagc gaaggagaac agcagggtca
     6661 ggttacagtt gataccttct ttttccagct gttctgcagc acggataccc tgccaggtag
     6721 aagccagttt gatcagaata cgatcgttgc taatacctgc atcgttgtag agtttgatca
     6781 ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga aagacgcgca tcaacttcag
     6841 ttgagatacg gcccggaacc agtttcagga tttccagacc aatatttact gccagtttgt
     6901 cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg ctgtttcgcc caggcgacag
     6961 catcatcaat caacttacgg tattccggaa tctgcgctgc gttaagaatg agagaagggt
     7021 tggttgtggc atcctgcggt tgatacagct tcattgccgc gatgtcccca gtgtcggcca
     7081 ctacggtggt gtactgacga agggaggtca atttgtccgt catgatagta tttctcttta
     7141 aacagcttgt tagggggatg taaccggtct gccctgatga tatcacgacg caacgtcagc
     7201 gcaaccgccg acaagacggt tatcgccagg cgagactgtt tcggatttct gacactgcgt
     7261 tgatatcgcc cgccattttt atacaaaacc tcatgtatgc tacgcagaag ttatcaagta
     7321 cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt acacaggaaa gtcatcgcga
     7381 ccggcaataa gagggatatg catgccagat tttttctcct ttattaacag cgtcctttgg
     7441 ggatcggtaa tgatttacct gctcttcggc gcaggttgtt ggttcacttt tcgcaccgga
     7501 tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc ttaaaaatag cattcatcca
     7561 cagccaggcg gtttaacctc atttcagtca ttgtgtacca gtcttgcggc gcgcgtgggt
     7621 agcggcaacc tggccggcgt tgcgctggca attaccgccg gtggaccagg tgccgtcttt
     7681 tggatgtggg ttgctgcgtt tatcggcatg gcgacctcgt ttgccgaatg ttcccttgca
     7741 caactttata aagaacgtga cgtcaatggg cagtttcgtg gcggaccggc atggtatatg
     7801 gcgcgcgggc tggggatgcg ctggatgggc gttctgttcg ccgtcttttt gctcatcgcc
     7861 tacggcataa ttttcagcgg agttcaggcg aacgccgttg cccgagccct gagtttttct
     7921 tttgattttc ctccgctggt gacaggcatt attctcgctg tctttgctct gctggcaatc
     7981 actcgcggtc ttcatggcgt cgcccggctc atgcaggggt ttgtcccgtt gatggcgata
     8041 atctgggtac tgaccagcct ggtgatttgc gtaataaata tcgggcaact tccccacgtc
     8101 atttggtcta tttttgagag tgcttttggc tggcaggaag cggcaggcgg cgcggcggga
     8161 tataccttaa gccaggcgat tactaacggt tttcagcgca gtatgttttc caatgaggcg
     8221 ggaatgggat cgacgccaaa cgcggcagcg gcagctgcgt cctggcctcc gcatccggca
     8281 gcacaaggga ttgtccagat gattggcatt tttatcgaca ccctggttat ctgtacggca
     8341 agcgccatgc tgatattact ggcgggtaac ggcacaacct acatgccgct ggaaggtatt
     8401 cagcttatcc agaaggcgat gcgggtgcta atgggtttct ggggtgctga atttgttacc
     8461 ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca actacattta tgccgaaaac
     8521 aatctcttct ttttacgcct gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc
     8581 ttcgcaacgg tcatcggcgg caccttgcta agccttccgc tgatgtggca actggcagat
     8641 atcataatgg cctgcatggc tattaccaat ttgaccgcca ttttactgct ctcgcctgtg
     8701 gttcatacca ttgccagtga ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt
     8761 gatccgttgc gttatccgga tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt
     8821 tcgcaggagt aatcacaact atcgatcaac tcattctctt tttttgctaa agtcggcata
     8881 aatttcctgc aaggactgga tatgctgatt cttatttcac ctgcgaaaac gcttgattac
     8941 caaagcccgt tgaccaccac gcgctatacg ctgccggagc tgttagacaa ttcccagcag
     9001 ttgatccatg aagcgcggaa actgacgcct ccgcagatta gcacgctgat gcgcatcagc
     9061 gacaaactgg cgggtatcaa cgccgctcgc tttcatgact ggcagccaga tttcacgccg
     9121 gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg tctacaccgg cttgcaggcc
     9181 gaaaccttca gcgaagacga tttcgatttt gcccaacagc atttgcgaat gctttccggc
     9241 ttgtatggcg tactgcgccc gctcgattta atgcagcctt atcgtctgga aatggggatc
     9301 cgtcttgaga atgcccgagg gaaagatctg tatcaattct ggggagatat catcaccaac
     9361 aagctgaacg aggcgctcgc agcacaaggc gataatgtgg tgattaatct ggcgtcagat
     9421 gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg agattatcaa gccggtgttc
     9481 ctcgatgaga agaacggcaa atttaagatc atcagcttct acgctaagaa agcacgcggt
     9541 ctgatgagtc gtttcattat tgaaaaccgg ctgaccaaac cagagcaact gactggtttt
     9601 aatagcgaag gttacttctt tgatgaagat tcctccagca atggcgaact ggtgtttaaa
     9661 cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc ggcgtgaacg ccttatccgg
     9721 cctacaaaag catgcagatt caatatattg cagaattaac gtaggcctga taagacgcgc
     9781 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
     9841 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
     9901 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
     9961 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
    10021 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
    10081 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
    10141 tatcattgcc cgttatgggt cgatatccgg agattacgcg atgagaacga cggttgttat
    10201 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
    10261 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
    10321 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
    10381 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
    10441 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
    10501 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
    10561 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
    10621 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
    10681 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
    10741 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
    10801 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgaatgactt cgccagcaga
    10861 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgcc
    10921 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
    10981 atgttaatcg agttagccga gtttaacccc agcgccactt tcagttcttc atcatcaaac
    11041 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
    11101 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
    11161 aggataacca ctttcacatt cggcaaaccg tagaaagcat gagccactgc agctccggta
    11221 tcaccggagg tcgcggtcag aatggtcact ggcttatcat ccgcaatatg ggtcagcatt
    11281 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
    11341 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
    11401 acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa taaacgccga gaggatcttc
    11461 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
    11521 ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
    11581 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
    11641 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
    11701 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
    11761 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
    11821 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
    11881 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
    11941 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagcccgg
    12001 gcttctgccg tcgagacttt aattcccgga tacgccagca cccacagcca ctcatcaaac
    12061 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
    12121 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga tacgtccttc cagctcgccc
    12181 atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc agtgttcatt catcgccatc
    12241 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgatcgg catattcttt
    12301 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
    12361 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
    12421 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
    12481 acgggtgcca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
    12541 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
    12601 ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
    12661 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
    12721 cagcggatca ttcccatcca cttcggcaat cttcacgcgg cagacaccat cttcatcaat
    12781 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
    12841 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cggcgttaaa
    12901 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
    12961 acgagcgaga atcaatagtt tacgcgccac atccatacca gaaagatcat ctcgcgggtc
    13021 cggttcggta taacccattt cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc
    13081 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcaccaa
    13141 ttcatcacct gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
    13201 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcataacgca actgatgata
    13261 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
    13321 caggaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
    13381 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
    13441 ggcttgtgca agttcttcct gccagttttc cagattaagg ccatgcacac tagtgagcag
    13501 agccttcgag ttggcaacgc cgcagacacg taagtcgata tgtttattct tcagccagct
    13561 ttgctgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
    13621 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
    13681 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
    13741 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
    13801 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
    13861 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagctcg
    13921 cacacagtcg ctttgtggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
    13981 cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag acgcgcgccg ccatgccaac
    14041 catccctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
    14101 gcccttgacc ggtaattcgt cttcatcacg gctggcacca atgagcgtac ctggtgcttg
    14161 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
    14221 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
    14281 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
    14341 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
    14401 gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt tcattaccgg cggtgaaacc
    14461 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
    14521 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
    14581 gataacggta acgttgtgac cacgcgcttc taacacgccg gccataatgg cgatcgacat
    14641 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
    14701 aatgccatcc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
    14761 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
    14821 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
    14881 caccaggtgg ttggtgattt tggcgggggc agagaagacg gtggccacct gcccctgcct
    14941 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
    15001 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
    15061 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
    15121 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
    15181 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
    15241 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
    15301 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
    15361 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
    15421 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgctg taaccagtcg
    15481 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
    15541 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
    15601 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
    15661 gccaccatcg gcacaccagt aagcacgtca gccaacgcta actcttcgtt agtcagcccg
    15721 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
    15781 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact acgcgcagtg
    15841 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gtgtcgggaa aactttaata
    15901 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgtgcc
    15961 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
    16021 ccaatatttt ctgctctggc gggtgcgacc aggataatcg ttatacgcat attgccactc
    16081 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
    16141 tacaaattct taacgtaagc cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
    16201 tattttctga ctgcactaac ggtttaattg ttaaaaaatg ctacatatcc ttctgtttac
    16261 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
    16321 atgatgcaac tagttggatt attaaaatta tgtgacgaaa gctagcattt agatacgatg
    16381 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
    16441 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
    16501 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
    16561 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
    16621 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
    16681 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
    16741 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
    16801 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
    16861 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
    16921 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
    16981 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
    17041 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
    17101 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat
    17161 cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt
    17221 ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg
    17281 tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
    17341 cgaatctacg ccggatacgc cggaaatcat cgccaccatt cacggtgaag gttatcgctt
    17401 ctgcggtgat ctggaagatt aatcggcttt accaccgtca aaaaaacggc gctttttagc
    17461 gccgttttta tttttcaacc ttatttccag atacgtaatt catcgtccgt tgtaacttct
    17521 ttactggctt tcattttcgg cagtgaaaac gcataccagt cgatattacg agtcacaaac
    17581 atcatgccgg ccagcgccac caccagcaca ctggttccca acaacagcgc gctatcggca
    17641 gagttgagca gtccccacat cactccatcc agcaacaaca gcgcgagggt aaacaacatg
    17701 ctattgcgcc agcctttcaa taccgcctgc aaataaatac cgttcattaa cgccccaatc
    17761 agactggcga ttatccatgc cacggtaaaa ccgatatgtt cagaaagcgc cagcaagagc
    17821 aaataaaaca tcaccaatga aagccccacc agcaaatatt gcattgggtg taaacgttgc
    17881 gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca gtgcaatcag cagaatggcg
    17941 tacttagtcg cccggtccgt taattggtat tgatcggctg gcgtcgtcac tgcgacgcta
    18001 aacgcgggga agttttccca gccggtatca ttgcctgaag caaaacgctc accgagatta
    18061 ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg actcgctagc ttcccgtttg
    18121 gctggtagaa aatcacctaa aaaactggga tgcggccagt tgctggttaa ggtcatttcg
    18181 ctattacgcc cggcaggcac cacagaaaga tcgcctgtac cgcttaaatt cagtgccata
    18241 ttcagctgca ggttctgctt ccgccagtcc ccttcgggta aagggatatg cacgccctgc
    18301 ccgccttgct ctaacccggt accgggttca atggtcagtg ccgttccgtt aacttcaggc
    18361 gctttcacca caccaatacc acgcgcatcc ccgacgctaa tcacaataaa gggcttgcct
    18421 aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa catcgaaatc ggcttttagc
    18481 gttaaatcac tgtgccagac ctgaccggta taaatcccta tcttgcgttc ttccacgttc
    18541 tgattgccat caaccatcaa tgactcaggt aaccaaaaat ggataaaact tcgtttccgc
    18601 tccacggttt tatcctcttc ctgcaccgta taaagctcgg tcacaggaat agcgataagc
    18661 ggcccaacga gtttttgcgg cccgctggta ctttggcgaa tcgcatcttc cacatcgcta
    18721 cggtaatcgg cacgttcgac aatcacttgc cgaatcagca ttatcggaat caacaacagc
    18781 aatactgcac caaacaaggt ggtcattttc cagaacaggg gggatttcaa catagtcttc
    18841 tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa gaatttgaag ctatgtgaag
    18901 tgacggtgaa gtcgtagcga ggccagcacg ccaccttcct gcacgttgcg cagcgtgact
    18961 tcgccgttaa acaaacgggc gacctcactg acgaacgcca accccagacc gctgcttttt
    19021 tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa tacgtgacag cgcgtagtca
    19081 ggaataccac taccggtatc cagcacctta agcgtgacgt gttcctgatc cacttcggcg
    19141 cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg cgttatccag taaattcccc
    19201 agcgcctgat ccagtaacgc cggttcagca gcaacattaa cctcggaggg cataacatgc
    19261 aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt cgctgacgcg gcgaaataat
    19321 gccgccacat caacagcagt cagaacgact tcctgacgat tctccagtct tgcctggcgt
    19381 agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg tcagaatgtt gtcagtaaaa
    19441 cgagccacta cttcaggcgg cggaccttcg cgtaaaattt ccgccgcgcc acgaatcgcc
    19501 gccagtgggc tttttagctc atgggttaac gcataaacat actgctcaat atagtttttc
    19561 ccttccagct tcacgcgcat actttccagc gcctgcgcga gtttacgcag ctcgctacta
    19621 ccgagatcgg gaagaggaac gggcttattg tcagtgacgg aatcagcata gcgagtgagc
    19681 cgagcaatgg agcggttgat ccaccaaacc atgcctgcgc caatcaccag tgcaatcccc
    19741 aacaaaatgg cgctggccca taaaattcgc cgctcgctgc gtttaatgac cggagccatc
    19801 gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc gcgagccgtc cataatcggc
    19861 gcggcaacat acatcaccga actttcggga tcggcaggat tttgcaacgt gctgcgcgca
    19921 ccatactgac cacgcaacgt tagccagacg tcattccagc gcgaataatc ctgtccaacg
    19981 gctttattta ccgaatcgaa caatactttg ccatgcgcat cggtcatata gacatgatat
    20041 tcattgcgca ctttgttaat gccaccaata ttggcgcgaa acgggcgatg ttgtagctga
    20101 ttaaacgcct gcgccagttg cccatgcgtt gggtccccag agagcaaatc gggacgcgcc
    20161 agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct ccgttgctct tcgcacgccc
    20221 ggtttaactt ctttgacaaa aatagccagt acgaaccagg ccgccaccgc caccagtaaa
    20281 aaatagcccc gcagcaaccg catgccgata cgcattacaa gcccctcagg ctatatccca
    20341 tgccgcgatg agtattaatc ggtgaaagat cggggttgat ggcgcgcagc ttggcacgca
    20401 gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc ctgcgcatct tcccacacgc
    20461 tatccattag ttgctggcgg gaccagacgc ggcctggcga cttgagtaac gtcttcagca
    20521 ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa ccagctgatc tgcgccgcgg
    20581 gttcattcaa ttcaaaatga ccaatacgga tgacgggaga cggcgtcgag aacttcttca
    20641 cccgacgcag taaggtgcgc accctggcgc acacttcgcg gggtgaaaac ggtttagcca
    20701 catagtcgtc agcgccaatt tccagcccaa gcaggcgatc gacctcttca cttcgggccg
    20761 tcaggaacag tacaggtaac gccggatgga gcgccagtaa ctggcggcac aattcaaagc
    20821 cactgatatc aggcaaacca acgtcgagaa tcatgacgtc gggaacctgc tggcgagctt
    20881 tatccagcac cggcaaacct cgctcaaaga cctcgacggc aaaaccttcc tgctgcaaca
    20941 tgtagaccag cgtgtcggct atcccttgct catcttccac taaccagacc gtttcccgtt
    21001 gcataaatcg cctctgttat tgccgccacg gcatgacagg aaccgcgcta atcgcgtttt
    21061 tcggcgaacc ttctacaact ttgtcggagt aagccagata agcgagcgcg ttgcgtttgg
    21121 cgtcataaaa gcgcacgacc tgtaacgact taaagaccag ggacgtgcgt tttttgaata
    21181 ctacctcgcc ctgagctttg ccgtttttaa tacgatccga cagttcaatc ggcccgactt
    21241 gctgacaaga aatggccgca tcggaggtat cttctgccag gcccaatccc cctttaatac
    21301 caccggtttt cgcccggctc acataacagg tgacattttt cacatcggga tcgtcaaagg
    21361 cttccacaac aattttgtga tccgggccga tcattttaaa tacggtgtcg accgaaccaa
    21421 tctcttctgc atgagccaat ggccccagca ttatcaggct taaagacagg atcaaatgct
    21481 tgtatttcat attgttacca tttttatcta ttacacgagc aattagttcg tcacggtaaa
    21541 taacatattg attaagaaca aaatctcaat tcttttattt ccgtcaagcc ctaaaacata
    21601 ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa aatgctatta tccaattacc
    21661 tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt atggatcagg ccggcattat
    21721 tcgcgacctt ttaatctggc tggaaggtca tctggatcag cccctgtcgc tcgacaatgt
    21781 agcggcgaaa gcaggttatt ccaagtggca cttacagaga atgtttaaag atgtcactgg
    21841 ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg aaatcggcgg tcgcactacg
    21901 cctgactgcg cgtccgattc tggacatcgc gctgcaatac cgcttcgact ctcaacagac
    21961 atttacccgc gcattcaaga agcagtttgc ccagactcct gcactttacc gccgttctcc
    22021 tgaatggagc gcctttggta ttcgcccgcc gctacgtctg ggtgaattca caatgccaga
    22081 gcacaaattt gtcaccctgg aagatacgcc gctgattggt gttacccaga gctactcctg
    22141 ttcgctggag caaatctctg atttccgcca tgaaatgcgt tatcagttct ggcacgattt
    22201 tctcggcaac gcgccgacca ttccgccggt gctctacggc ctgaatgaaa cgcgtccgag
    22261 tcaggataaa gacgacgaac aagaggtatt ctataccacc gcgttagccc aggatcaggc
    22321 agatggctat gtactgacgg ggcatccggt gatgctgcag ggcggcgaat atgtgatgtt
    22381 tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc ctgacggtat acggaacgtg
    22441 catgccaatg ctcaatctga cgcgccgtaa aggtcaggat attgagcgat actacccggc
    22501 agaagacgcc aaagcgggag atcgcccaat taatctgcgc tgtgaactgc tgattcctat
    22561 ccgccgttaa cgctgcagct catctaatgc aggggcgtcc agatgcgaga tgtcccctgc
    22621 ggtttcaacg acccagccag acgccagcca caggctttcc tgataatcca cgcgcgaaat
    22681 agaacagtta cgcagacgta agcggcgctc tgcccatgct ggtaatccga gaatcgtact
    22741 caccagacat cccagtgcaa taccatggct caccagcagc ggtcggcttc cctgcggtaa
    22801 gtcccggcaa gattccagtg ccgcattgac gcgatcgctg agctcctgca ttgactcgcc
    22861 ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc cgacgccagt tctcttcttc
    22921 ttcggtcaga gaatcgatat gtcttttttc cagcacaccc atgtttaatt cacgcaggcg
    22981 agaatcaaag atgatgtcac agccgcaggc ctgggcgatg atttccgccg tacgccgggt
    23041 gcgtcctaaa tcgctactga taatatgcgt aatgccaagc tctttggcac gggttgccac
    23101 ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg tcagactggc cctgaatacg
    23161 tcgctcggcg ttccactgcg tttcaccgtg gcggactagg tatacctgta acatgctttt
    23221 tttccgtata ctgctgtaac gttaattttc tgagtaatgc tgattatgca ccaagttgtc
    23281 tgtgcgacca ccaatcccgc taaaattcag gccattctgc aggcatttca cgagatcttc
    23341 ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga gcggtgtacc ggaacaaccc
    23401 tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg tagcaaatgc ccgccgttta
    23461 cttccagagg ctgatttttg ggtggcgatt gaagctggca ttgatggcga cagcactttc
    23521 agttgggtgg tgattgaaaa cgccagccag cgcggcgagg cgcgttcggc aactttgccg
    23581 ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg cgcttggtcc cgtgatgtcg
    23641 cgttataccg gtattgatga gattggtcgt aaggaagggg cgatcggcgt gtttaccgcc
    23701 gggaaactca ctcgcgccag cgtctatcac caggcggtga tcctggcgct gagtccgttt
    23761 cacaatgccg tgtatcaggc attgtaggac ggataaggcg ttcacgccgc atccgacatt
    23821 cggtgcacga tgcctgatgc gacgctgccg cgtcttatca ggcctacaaa atcaatcgct
    23881 tttcagcaac acctcttcca gccactggcg cagctccacg ggcgcggctt tcaggctgtt
    23941 agatccacgc gtaatcgtcg cgatgcccgc gccgagttca ttttttaact cacgctgact
    24001 catttcgcca cgcaacagct cttcgacaat acgcacgcga gtccccaacg cttcgcgctc
    24061 atctggcgtc agcatcaggt ttaacaacgg taaatggaga tcgttttggt aggcattctt
    24121 aagcaggtcg acaaaacgta accactcctg gtgacgctgt tctgccatcg ctgctgaata
    24181 gggtgattgt tgggccataa tatgtcgcca ttgttagcgg gggaagcaaa atgactcccc
    24241 cggttgtact cgctaaagag tacgatagca tatcataaac gtgcggatca gtaacgacgt
    24301 ccccattccg tggcgctcat caacgtcggt ttatccccca tgaaatagcg gtagtaagca
    24361 tcataagcca gcacgttctt cacataaccg cgcgtctcgg agaacggaat actctcgaca
    24421 aatgccacag cgtcgatacg cccggcgctg tggccaagcc aggttcgcac ccgtccaggt
    24481 ccggcgttat aagctgctga ggagaaaata cggttattgc caaactgctg ataaacatat
    24541 tgcaggtaac tggtgccaat gttgatgttc gtttccggat ccagcaattg cccagggctg
    24601 ctataaccag gaatagagaa catcttcacc gtatgggtcg ctgtaccagg cataatctgc
    24661 atcaggccgc tggcccctac cggtgatttc actttcggat tccaggcgct ctcctgacgg
    24721 gcaatcgcca tcgcatagct ttgcgggatc tctttcccac tggtgtaacg tttgaaaaga
    24781 tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc aaagcttccc ggcgatcgtt
    24841 gcctgaacgc taagatccca ccattggtta ttgaaagcat accgagccag ttgagcctgc
    24901 tctgtttttg acttgctctt caccagattg gcccactcgc tacgcgcggt attatcgaga
    24961 ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc cctgagtcag ggtgctgtca
    25021 acattctgcg gcgctttatc aatcttcagc tcatactctt caccgatgcg ttgtgctgca
    25081 accatcgggt agaaaccacg ctgttgcatg agttgatgca aaatctcttt cgcttcagct
    25141 tcacgtccgc gttccagcaa taaatccgcc tgccagtaac gccattcatc tttctctttt
    25201 gcttccatcg gcagacgcgc cagccaggta ttcaggccgc gacgatcgcc ggtgccaagc
    25261 gccattcgca cacggcgttc aatcaacgac gtggattgcg agcgcataat ggcgtcatcg
    25321 cgccatttcg cctgctcatc ggtgacatca ttgcccatca aacgccaggc gacgatatcg
    25381 cgcagctcct gaatctgatc ttcattaagc tgctgcgcct gggcaagcga cgggatcatc
    25441 agccgtgcat tttcggcatc ctgccgcgcc acactggcaa acgccaccgc cgccatttga
    25501 cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca gtaccgtatt agggttgttc
    25561 gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg caggcatctg ccctgccagc
    25621 actgttacta ggcctgtgtt acccgctttc atcgccagac ggatacgctc taaatacgcc
    25681 agcggatctt gtttacctga cgcacgccag acgctaaata acttgtcaca ggcgttaggc
    25741 tggctcttgc cggttagcca cagctctttc gccccttgcc aggcttcttc actctgcccg
    25801 gtgttccatt tcgcatagta gtaattacat tgcgcttcgg tagttccggg cttttccggg
    25861 ctaaaggcta acaagccacg ccagtcttca cgccgcgcca gttcattgac gaaacgagat
    25921 tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa caaagttagt gaccgtcacc
    25981 gccggttgat tcatcaggtc atcggtgatc tggcggtatt ccaggtaggg ataaagcgga
    26041 taatccttca gtccaggcat catttgttcg accacatcca tctgtcgatt atcccaggcc
    26101 tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg agtcggctcg cgccacgctg
    26161 ctgaccgtca gcagacagac accggcagcc aacagccgcc aggtaacttg tttggctttt
    26221 tccacaagcg catcctctaa gtgtaaatac atcaatgcag cgtcatgccg cgtaatagat
    26281 ttatgctaac cagtcattgc cgtttacgcc acgttacgga cactttttta cttttactgc
    26341 gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc tacacttcgc ctttgaaaac
    26401 tcatcatcac gataaacata gaggcgaagt ccaacgtggc tcaattcgtt tataccatgc
    26461 atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt gaaaaacatc tctctgagtt
    26521 tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg cgcgggtaag tccaccctgc
    26581 tgcgcattat ggcgggcatt gataaagaca tcgaaggtga agcgcgtccg cagccagaca
    26641 tcaagattgg ttatctgccg caggaaccgc agctgaaccc ggaacacacc gtgcgtgagt
    26701 ccattgaaga agcggtttca gaagtggtta acgccctgaa acgcctggat gaagtgtatg
    26761 cgctgtacgc cgatccggat gccgattttg acaagctggc cgctgaacaa ggccgtctgg
    26821 aagagatcat tcaggctcac gacggtcata acctgaacgt acagctggag cgtgcggcgg
    26881 atgcgctgcg tctgccagat tgggacgcga aaatcgctaa cctctccggt ggtgagcgtc
    26941 gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga catgctgctg ctcgacgaac
    27001 cgaccaacca cctggatgcc gaatccgtgg cgtggctgga acgcttcctg cacgacttcg
    27061 aaggcaccgt ggtggcgatt actcacgacc gttacttcct cgataacgtt gcaggctgga
    27121 tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg taactactcc tcctggctgg
    27181 agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga agcggcgcgt cgtaagtcga
    27241 tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg ccgccagtcg aaaggtaaag
    27301 cacgtctggc acgctttgaa gagctgaaca gcaccgaata ccagaaacgt aacgaaacca
    27361 acgaactgtt tattccacct ggaccgcgtc tgggcgataa agtgctggaa gtcagcaacc
    27421 tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct gagcttctcg atcccgaaag
    27481 gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa atcgaccctg ttccgtatga
    27541 tctccggtca ggaacagccg gacagcggca ccatcacttt gggtgaaacg gtgaaactgg
    27601 catcggttga tcagttccgt gactcaatgg ataacagcaa aaccgtttgg gaagaagttt
    27661 ccggcgggct ggatattatg aagatcggca acaccgagat gccaagccgc gcctacgttg
    27721 gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt tggcgaactt tccggtggtg
    27781 agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg cggcaacatg ctgctgctcg
    27841 acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc gctggaaaac gccctgctgg
    27901 agttcccggg ctgtgcgatg gttatctcgc acgaccgttg gttcctcgac cgtatcgcca
    27961 cgcacatcct ggactaccag gatgaaggta aagttgagtt cttcgaaggt aactttactg
    28021 agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc gctggagccg aagcgtatca
    28081 agtacaagcg tattgcgaag taatgcgtaa aatgccggat gcggcgcgaa cgccttatcc
    28141 ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt aggcctgata agacgcggta
    28201 agcgtcgcat caggcattga gcaccgaatg ccggatgcgg cgtgaacacc ttttccggtc
    28261 tacaacagcc ttagcatcct caatttcgtg gcgggtcacc cgtgcaggtt ttttcaaacc
    28321 ttgtttaatc tcgacagcct cttccagaga ggttttcaac tcatcaaaaa aactcatttt
    28381 atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc tcccccatca tctcccgcac
    28441 cagctccacg cagcgcagga aacggctgtc gtaatcgtcc tcttcaaccc gcacgaattc
    28501 gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga
    28561 actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac
    28621 cagatcgaaa cggtattcat caatcagcgc ctgcacgaac ggatgctcac gcccttcgta
    28681 ctttttgcag aacgcctggg tagtgacaaa atcggtgtca ataaacgcca ctttattggc
    28741 gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac gcgattttat cgtagtcgga
    28801 atactgcaat gcgatctcat caccgccgag gtgtgaaaag acataatcgc ggccatattc
    28861 ccacgcactg gtggtgttga agatattggc aagtttgttt accagggtgg atttaccgct
    28921 cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac ggcttcactt cggtaggaat
    28981 atattcccag tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt
    29041 acgtttcgga tcgaccagca ccgtctcgat ccccagatgt tccatatact gtggcgcatc
    29101 ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc ttttcagcca taaacttttt
    29161 gatgccgttg ctccacacat cccagccatg cggatacggc tccatgccct cttcgttgaa
    29221 agcatgaata cggatatttt tctgatattt aaaggtttgc aatagccagc gcagacgatc
    29281 cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc
    29341 gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta
    29401 gatatgtccg gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg
    29461 ccggggaaat tcaagcccca aaaaacggtg caatgcctcc agcttttgcg cgctggggct
    29521 tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc
    29581 tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga
    29641 catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt
    29701 tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga
    29761 acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa tccgctcctg gccactgggc
    29821 accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc
    29881 ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaagtcggc gctggtttcg
    29941 gttaccttca cgccgccgac cacgttcaca aacacatcct gatcggccat ttgcagacca
    30001 ccgtgacggt gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc
    30061 acgcggcgcg gattcgccat tatcgagtga tcgaccagcg cctgaatctc caccagcaac
    30121 ggacgcgttc cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga
    30181 cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag
    30241 acgcccagct cattcaccgc gccgaagcgg tttttatggc tgcgtaaggt gcgaaaacgg
    30301 gagtcggcat cgccatccag aagcaccgaa cagtcgatac agtgttccag cactttcggg
    30361 ccagccagcg aaccatcttt ggttacgtgt ccaaccatga caatcgccac accgcgcgtt
    30421 ttggcgaaac gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat
    30481 gattgtacat ccgccatatg catcacctgg atcgagtcaa ttaccatcag cttcggttgc
    30541 tcttcttcgg caatcaggca gatctgctcg atgctggttt ccgacaacat attgagattg
    30601 tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgccg
    30661 gtgacataca gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag caacagcgtg
    30721 gatttccccg caccagggtt accgccaatc agaatggcac ttcctggcac cacgccgccg
    30781 cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg
    30841 atatcggaga gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc
    30901 tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca
    30961 tgacaggcac tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca
    31021 aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga
    31081 tgacgggcag gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc
    31141 aggtcagcgt gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag
    31201 gcaatcccca gccctgctgc tttgatcatc tgcaggtcat tagctccatc gccaatcgcc
    31261 acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcagagt tttcgctttg
    31321 tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc
    31381 agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc gcaggtattc agcaaagaaa
    31441 gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagc
    31501 tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca
    31561 gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg taaaatcgag ttcgccgcgc
    31621 atcgcccgtt cggttacctc cgccaccatc tcgcccgttc cggccagctt ggcaatttca
    31681 tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg
    31741 cgcaggtgcg ggattttccc cagcggggcg acatccagct gcgcttcgtg ggccaggcgt
    31801 gtagcccgtg cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag
    31861 gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta
    31921 tccagcccac gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc
    31981 acttcatcac cacttaatga aagaggcaga cccggccata aagagacatc ttcaggcagg
    32041 tcgcaccagg taatgttagg cattaaggct ccagtaaaat cgttcgaagc agggaaaata
    32101 acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag
    32161 ggtggaagat ggctcgcaca aaactgaaat tccggctaca tcgggcagtg attgtcctgt
    32221 tctgtcttgc cttgttagtg gcactgatgc agggggcgtc atggtttagt caaaaccacc
    32281 agcgacagcg taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtgacgc
    32341 tgaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc
    32401 tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg
    32461 atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga tcggctggcg ctcgacggta
    32521 aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc aattgcgggt aaaaacggac
    32581 cgctcggcta tctgcgcctg acactcgaca cccatacgct cgccaccgaa gcccaacagg
    32641 tggataacac cactaacatt ttacgcctga tgttgctgct ctcactggca atcggcgtag
    32701 tgctgacccg cacgctgcta cagggtaaac gcacccgctg gcagcaatcg cccttcctgt
    32761 taaccgccag caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta
    32821 ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc
    32881 cgtggtttaa cctggcggtg gaagagtgta tttttcgcca aatgcccgcc acgcagcgcg
    32941 ttctgtttct ctggcgcaat gccgacacgg tagtaattgg tcgcgcgcag aacccgtgga
    33001 aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcgcgg cgcagtagcg
    33061 gtggcggcgc ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc
    33121 cggagtacga taaaactatc tccacgtcga ttgtgctcaa tgcgctgaac gcgctcggcg
    33181 tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa aaccgtcgaa ggcgaccgca
    33241 aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg cttccaccac ggcaccttgc
    33301 tactcaatgc cgacctcagc cgcctggcaa actatctcaa tccggataaa aagaaactgg
    33361 cggcgaaagg cattacttca gtgcgtttcc gcgtgaccaa cctcaccgag ctgctgccgg
    33421 ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    33481 agcgcgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    33541 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    33601 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    33661 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    33721 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    33781 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    33841 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    33901 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    33961 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    34021 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    34081 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    34141 gaacataaat gaatagattc gtgaatagat acttgaatgt attttatttt ctatccatat
    34201 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    34261 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    34321 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    34381 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    34441 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    34501 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    34561 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    34621 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    34681 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    34741 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    34801 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    34861 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    34921 gagcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    34981 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    35041 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    35101 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    35161 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccataca ccagtaccgg
    35221 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    35281 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    35341 cagttccaga cccgcagcat aaccggcgac gtcgcgacgg tggccccagg aagagtcgaa
    35401 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    35461 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    35521 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    35581 ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg tgacggttac cggtacgctg
    35641 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    35701 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    35761 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    35821 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    35881 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    35941 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    36001 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    36061 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    36121 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    36181 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    36241 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    36301 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    36361 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgctt tatcggcaag
    36421 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgctag
    36481 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    36541 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaaccac
    36601 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    36661 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    36721 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    36781 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcagcat
    36841 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    36901 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    36961 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    37021 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    37081 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    37141 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    37201 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    37261 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    37321 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    37381 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    37441 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    37501 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    37561 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    37621 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    37681 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    37741 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    37801 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    37861 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    37921 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    37981 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    38041 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    38101 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    38161 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    38221 ggctttcact aggtcaaaac caacctgctc gttacccgcc atcagcgcgc ggtacgggaa
    38281 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    38341 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    38401 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    38461 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    38521 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    38581 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    38641 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    38701 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    38761 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    38821 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    38881 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    38941 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    39001 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    39061 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    39121 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    39181 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    39241 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    39301 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    39361 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    39421 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    39481 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    39541 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    39601 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    39661 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    39721 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    39781 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    39841 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    39901 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    39961 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    40021 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    40081 attattattc cggcatcagc gacattctga cgctcgacga aatcatcaaa cgtaacccgc
    40141 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    40201 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    40261 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    40321 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    40381 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    40441 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    40501 tgcaatggct gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    40561 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    40621 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    40681 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    40741 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    40801 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    40861 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    40921 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    40981 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    41041 gaactggcgg cgtttatcaa gggacttggc gatgttccgg ttcgcctgaa cgcgtttcat
    41101 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    41161 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    41221 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    41281 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    41341 gcctacggtt cggcacaaac ctgtaggcct gataagacgc gttaagcgtc gcatcaggca
    41401 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    41461 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    41521 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    41581 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    41641 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    41701 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    41761 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    41821 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    41881 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    41941 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    42001 gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg gttgataatt
    42061 ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    42121 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    42181 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    42241 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    42301 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    42361 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    42421 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    42481 aatggcttcg gcggataaat ttcgaatatc cgcagcttgc ccggtggttt ctcaataaat
    42541 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    42601 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    42661 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    42721 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    42781 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    42841 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    42901 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    42961 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    43021 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    43081 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    43141 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    43201 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    43261 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    43321 aaagacaaaa taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    43381 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    43441 actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    43501 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    43561 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    43621 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    43681 gggaggacga attacgacga attacttagt tttcagatca tttttaacgc ttttcacacc
    43741 atctaccgct ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc
    43801 ggagagctga accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc
    43861 gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt
    43921 caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc
    43981 aacgcctttc gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct
    44041 cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg
    44101 atccaccagc gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac
    44161 tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt
    44221 ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt
    44281 cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga
    44341 gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca
    44401 atggtcgcca tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag
    44461 ttattaatat cggtagaatg acaatcgacg gctttagata ctgatatcta cgcattgaac
    44521 ggtatttaac gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac
    44581 cgctcgggat atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc
    44641 ggctaataag gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat
    44701 taatgctcac gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg
    44761 ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt
    44821 tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca
    44881 gtggcaacgt tgactgactc atacactgcc tcaacgttgt attcgctctt caggcgcgct
    44941 accaccacat caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt
    45001 ggacggaaca cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt
    45061 ttttgcttca gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga
    45121 ataccggtga acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg
    45181 tggttgtgca ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc
    45241 gccataaagg tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt
    45301 ttcatgcctt tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt
    45361 ttcgggtcca tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct
    45421 tctacggtac gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca
    45481 tccagcatat gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt
    45541 tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt
    45601 tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc
    45661 agccctttaa caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag
    45721 gtttcatctt tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccag
    45781 gtgatcggtg cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg
    45841 tcgcggatat cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg
    45901 gtaacttcca tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc
    45961 aggcagcagt ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc
    46021 ggggtgtcga gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta
    46081 atggagatcc cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa
    46141 ccacggcctt ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg
    46201 gtgatggtag ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc
    46261 acctcttgca aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg
    46321 ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat
    46381 cagtgtttac acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg
    46441 atgccttctg gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg
    46501 atgccaccga gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca
    46561 ggattgcccg cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca
    46621 acttcttcgg aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc
    46681 acttgctgca aggcgctaaa accgttggtg atgatgccga ttttagcgtt gccacgaatg
    46741 gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata
    46801 aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc
    46861 ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg
    46921 ttaacggcct gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac
    46981 atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa
    47041 atccagtccc acttcattat tccaccttgt cttacatact gattggcaac gccatgatga
    47101 tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa
    47161 agcctaaact ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata
    47221 gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca
    47281 gcccctgacg ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca
    47341 ccacttgcgt aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat
    47401 aacgctcgcc ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt
    47461 gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat
    47521 gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc
    47581 gccggtgatg ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag
    47641 ctgttgcagc gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact
    47701 tgatcggggc tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg
    47761 ggaagatcgt ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc
    47821 ccctgcaacg cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc
    47881 cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa
    47941 ttcgtcgata ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt
    48001 ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac
    48061 aaagccgtat tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg
    48121 cggccagccg tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg
    48181 gggataacgc ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc
    48241 tgatttacta ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt
    48301 ctctgctgcc agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca
    48361 gcgctgagca gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct
    48421 gtggcctcta ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg
    48481 gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg
    48541 gtctggatgt cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag
    48601 tgctggatgt cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga
    48661 aaattcgtct caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac
    48721 ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag
    48781 gtcgttttga tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg
    48841 atgcggcgca aacgctgatt cgcggcgcgg tgcgtcatct taatagcggc ggcgagctgc
    48901 gaattgtagc gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc
    48961 atgaagttat cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg
    49021 cgaagaaagg ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac
    49081 gccttatccg acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca
    49141 gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg
    49201 gacatttttc cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac
    49261 cactagaatg cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DADLSE010000012        48347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLSE010000012 DADLSE010000000
VERSION     DADLSE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.012"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN692_000717"
     CDS             complement(<1..209)
                     /locus_tag="JN692_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7263106.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN692_000718"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN692_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7263107.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN692_000719"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN692_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7263108.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN692_000720"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN692_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7263109.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN692_000721"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN692_000721"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7263110.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN692_000722"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN692_000722"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7263111.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN692_000723"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN692_000723"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7263112.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN692_000724"
     CDS             complement(4598..5320)
                     /locus_tag="JN692_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7263113.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN692_000725"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN692_000725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7263114.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN692_000726"
     CDS             6520..6738
                     /locus_tag="JN692_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7263115.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN692_000727"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN692_000727"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7263116.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN692_000728"
     CDS             complement(7472..7672)
                     /locus_tag="JN692_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7263117.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN692_000729"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN692_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7263118.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKTQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN692_000730"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN692_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7263119.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JN692_000731"
     CDS             10169..12082
                     /locus_tag="JN692_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7263120.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JN692_000732"
     CDS             12082..13104
                     /locus_tag="JN692_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7263121.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JN692_000733"
     CDS             13098..13316
                     /locus_tag="JN692_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7263122.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JN692_000734"
     CDS             13370..14239
                     /locus_tag="JN692_000734"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7263123.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="JN692_000735"
     CDS             complement(14294..14698)
                     /locus_tag="JN692_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7263124.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="JN692_000736"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="JN692_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7263125.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="JN692_000737"
     CDS             15683..17773
                     /locus_tag="JN692_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263126.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN692_000738"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN692_000738"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7263127.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGWARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN692_000739"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN692_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7263128.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="JN692_000740"
     CDS             complement(19741..20343)
                     /locus_tag="JN692_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7263129.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="JN692_000741"
     CDS             complement(20333..20500)
                     /locus_tag="JN692_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7263130.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN692_000742"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN692_000742"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7263131.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="JN692_000743"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="JN692_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7263132.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNNAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /locus_tag="JN692_000744"
     CDS             22891..25434
                     /locus_tag="JN692_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7263133.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="JN692_000745"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="JN692_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7263134.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="JN692_000746"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="JN692_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7263135.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="JN692_000747"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="JN692_000747"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7263136.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLQEAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="JN692_000748"
     CDS             28328..28495
                     /locus_tag="JN692_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7263137.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30022
                     /locus_tag="JN692_000749"
                     /pseudo
     CDS             28790..30022
                     /locus_tag="JN692_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            30043..31065
                     /gene="frlB"
                     /locus_tag="JN692_000750"
     CDS             30043..31065
                     /gene="frlB"
                     /locus_tag="JN692_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7263138.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31115..31945
                     /gene="frlC"
                     /locus_tag="JN692_000751"
     CDS             31115..31945
                     /gene="frlC"
                     /locus_tag="JN692_000751"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7263139.1"
                     /translation="MKTGMFTCGQQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31942..32727
                     /gene="frlD"
                     /locus_tag="JN692_000752"
     CDS             31942..32727
                     /gene="frlD"
                     /locus_tag="JN692_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7263140.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32827..33558
                     /gene="phoB"
                     /locus_tag="JN692_000753"
     CDS             32827..33558
                     /gene="phoB"
                     /locus_tag="JN692_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7263141.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33718..34803)
                     /locus_tag="JN692_000754"
     CDS             complement(33718..34803)
                     /locus_tag="JN692_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7263142.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34815..36119)
                     /locus_tag="JN692_000755"
     CDS             complement(34815..36119)
                     /locus_tag="JN692_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263143.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36131..36484)
                     /locus_tag="JN692_000756"
     CDS             complement(36131..36484)
                     /locus_tag="JN692_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HAY7263144.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36495..37373)
                     /locus_tag="JN692_000757"
     CDS             complement(36495..37373)
                     /locus_tag="JN692_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7263145.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37370..38596)
                     /locus_tag="JN692_000758"
     CDS             complement(37370..38596)
                     /locus_tag="JN692_000758"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7263146.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38597..39760)
                     /locus_tag="JN692_000759"
     CDS             complement(38597..39760)
                     /locus_tag="JN692_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7263147.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRGQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39844..40206)
                     /locus_tag="JN692_000760"
     CDS             complement(39844..40206)
                     /locus_tag="JN692_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7263148.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40223..41128)
                     /locus_tag="JN692_000761"
     CDS             complement(40223..41128)
                     /locus_tag="JN692_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263149.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN692_000762"
     CDS             complement(41398..42402)
                     /gene="trpS"
                     /locus_tag="JN692_000762"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7263150.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN692_000763"
     CDS             complement(42395..43153)
                     /gene="gph"
                     /locus_tag="JN692_000763"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7263151.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN692_000764"
     CDS             complement(43146..43823)
                     /gene="rpe"
                     /locus_tag="JN692_000764"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7263152.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN692_000765"
     CDS             complement(43841..44677)
                     /gene="dam"
                     /locus_tag="JN692_000765"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7263153.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN692_000766"
     CDS             complement(44784..46070)
                     /gene="damX"
                     /locus_tag="JN692_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7263154.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTELAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQVDLK"
     gene            complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN692_000767"
     CDS             complement(46162..47250)
                     /gene="aroB"
                     /locus_tag="JN692_000767"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7263155.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPRKMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN692_000768"
     CDS             complement(47307..47828)
                     /gene="aroK"
                     /locus_tag="JN692_000768"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7263156.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48229..>48347)
                     /locus_tag="JN692_000769"
     CDS             complement(48229..>48347)
                     /locus_tag="JN692_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263157.1"
                     /translation="VPLLGDIPWFGQLFRHDGKEDERRELVVFITPRLVSSE"
BASE COUNT        11644 a        13150 c        12088 g        11465 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag tgccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccaaga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctag tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcaatgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaagccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggctcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctgggtttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggccca ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccttttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttac ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccaaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac aacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccag cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgca ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 ttgataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacata acatcatatg ttgttatatg catcatgcat tgtcatgtta ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg attggttcct gtctgctggt
    29401 tctggtgctc tacaccctgc tggcgctggt gatttccggc ctgatgccct tcgacaaact
    29461 cgccaattct gaaacgccga tttccgacgc cctgacctag atccccgcac tcggcagcac
    29521 cgctgggatc tttgttgcca tcacggcgat gatcgtcatt cttggttcgc tttccagctg
    29581 cgtgatgtac cagccgcggc tggaatacgc gatggcgaaa gacaacctgt tctttaaatg
    29641 cttcggccat gtgcatccga aatacaacac gccggatgtc tccatcatcc tgcaaggggc
    29701 gctggggatc ttcttcatct tcgtttccga tctcaccagc ctgctgggtt atttcaccct
    29761 ggtgatgtgt ttcaaaaata ccctcacctt cggctccatc atctggtgtc gtaaacgcga
    29821 cgattacaaa ccgctgtggc gtactccggc tttcgggctg atgaccaccc tcgccattgc
    29881 gtcaagcctc attctggtcg cctcaacctt tgtctgggca ccgattcccg gccttatctg
    29941 cgccgtcatc gttattgcta ctggtctgcc tgcttacgcc ttctgggcga agcgtagccg
    30001 ccagctcaac gctttgtcgt aatgttatct gaagaaaaaa aaatgttgga tattgataaa
    30061 agcaccgtgg actttctggt caccgaaaat atggttcagg aagtggaaaa agttctcagc
    30121 catgacgttc cgctggtgca cgccatcgtg gaagagatgg tgaagcgcga cattgatcgt
    30181 atttatttcg ttgcctgcgg ctcgccactc aacgcggcgc aaacggcgaa acatctggcg
    30241 gatcgctttt ccgatcttca ggtctacgcc atttccggct gggagttctg cgataacacc
    30301 ccgtatcgcc tcgacgatcg ttgcgcggtg attggcgttt ctgactacgg taaaacggaa
    30361 gaggtaatca aagcgctgga gctgggccgg gcctgcggcg cactcactgc ggcgttcacc
    30421 aaacgcgcgg acagcccgat tacctcggcg gcggaattta gcattgatta tcaggccgac
    30481 tgtatctggg aaattcacct gctgctctgc tacagcgtgg tgctggagat gatcacccgc
    30541 ctcgcgccga acgcggaaat cggcaagatc aaaaacgatc tcaagcagtt gccgaatgcg
    30601 ctcggtcatc tggtacgcac ctgggaagaa aaaggccgcc agcttggtga actggccagc
    30661 cagtggccga tgatttatac cgttgctgcg ggtccgctgc gtccgctggg ttacaaagaa
    30721 ggcattgtaa cgctgatgga atttacctgg acgcacggct gcgtgattga gagcggagag
    30781 ttccgccatg gcccgctgga gattgtcgaa ccgggcgttc cgttcctgtt cctgctcggc
    30841 aatgatgaaa gtcgccacac caccgaacgc gccattaact ttgttaaaca gcgtactgac
    30901 aacgtgatcg tcatcgatta cgccgaaatt tcgcaagggc tgcacccgtg gctggcaccg
    30961 ttcctgatgt tcgtgccaat ggagtggctc tgctactacc tgtctattta caaagatcac
    31021 aacccggatg aacgccgcta ttacggtggt ctggtggaat attaatccct ctcccggccc
    31081 ggcaacgggc cggatttacg caaggagtta cccgatgaaa acaggtatgt ttacctgcgg
    31141 ccagcagcgg ctgcctattg aacacgcatt tcgtgatgca agcgagctgg gttacgacgg
    31201 catcgaaatt tggggcggtc gcccgcacgc gttcgcgccg gacttaaaag cgggcggcat
    31261 caaacaaatc aaggcgctgg cgcagacgta tcagatgccg attatcggtt acacgccaga
    31321 aaccaacggc tatccgtata acatgatgct gggcgatgaa catatgcgtc gtgaaagcct
    31381 ggacatgatc aagctggcga tagatatggc aaaagagatg aacgcgggtt atacgctgat
    31441 ttctgcggcc cacgcgggct atctcacgcc gcctaacgtt atctgggggc ggctggcaga
    31501 gaacttaagc gaactgtgtg agtacgcgga aaacatcggc atggatctga tcctcgaacc
    31561 gttaacgccg tatgaatcga acgtcgtgtg taatgctaat gatgtgcttc atgcgctggc
    31621 gctggtgcct tcgccgcgcc tgttcagcat ggtcgacatt tgcgcgccgt atgtccaggc
    31681 ggaaccggtg atgagttatt tcgacaaact gggcgataaa ttacgtcatc tgcatattgt
    31741 cgacagcgac ggggccagcg acacgcatta cattcctgga gaaggcaaaa tgccgctgcg
    31801 ggaactgatg cgcgatatta ttgatcgggg ctatgagggt tactgtacgg tggagctggt
    31861 gacgatgtat atgaacgagc ccagactcta tgcccgccag gcgctggaac gctttcgcgc
    31921 gctgctgccg gaggatgaga gatgaaaacc ctggcgacaa tcggcgataa ctgcgtcgat
    31981 atctacccgc aactgaataa agcgttttct ggcggtaatg cggtcaatgt ggcggtgtac
    32041 tgcactcgct acggcataca gccgggatgc attacctggg tgggtgacga tgactacggc
    32101 acaaagctga agcaggatct cgcccgcatg ggcgtcgata tcagccatgt ccatacgaaa
    32161 cacggcgtta ccgcacaaac tcaggtggaa ctgcacgaca atgatcgcgt ttttggcgac
    32221 tacaccgaag gcgtgatggc cgactttgcc ctgagtgaag aggattacgc ctggctggcg
    32281 cagtatgaca ttgtgcacgc ggcaatctgg ggacatgcgg aagacgcatt cccacagctg
    32341 cacgctgcgg gcaaacttac cgctttcgac ttctccgaca agtgggacag cccgctctgg
    32401 cagacactgg tgccgcatct cgattttgcc tttgcctccg caccgcaaga agacgaagcg
    32461 ctgcgtctga agatgaaagc gattgttgcc cgtggcgcag gaacagtgat tgtcacgctg
    32521 ggtgaaaacg gcagcattgc ctgggatggc gcgcagttct ggcgtcaggc tcctgaaccg
    32581 gtgacggtta tcgacaccat gggtgccgga gattcgttca ttgccggatt cctttgcggc
    32641 tggtctgcgg ggatgacatt accgcaggcg atggcgcagg gaacggcgtg cgcggcgaaa
    32701 accattcagt accacggtgc ctggtaggta taacgttggc gtgagcatct tcacgccaac
    32761 gtgctgttac ttgccggaaa acgaccctat aatccgagta attcattctt tatttcaggg
    32821 ccgattatgt cagctacgga ccgctactct catcaactcc tctacgctac cgtccgccag
    32881 cggctgctgg atgatatcgc gcagggggtt taccaggccg ggcaacagat ccctaccgaa
    32941 aacgagcttt gtacacaata taacgtcagc cgcattacca ttcgcaaagc catcagcgac
    33001 ttagtggcag acggcgtact gatccgctgg cagggaaaag gcacctttgt acaaagccag
    33061 aaagttgaaa acgccctgct tactgtcagt ggttttaccg attttggcgt ctcacaaggc
    33121 aaggcgacga aagagaaagt gatcgaacag gaacgggtca gcgccgcgcc gttttgcgaa
    33181 aagctgaaca tccccggaaa cagcgaagtg ttccatctct gccgggtgat gtatctcgat
    33241 aaagagccgc tgtttattga tagttcatgg atcccgctgt cgcgttatcc tgactttgat
    33301 gagatttacg tcgaaggaag ctccacctat cagttatttc aggagcgttt tgacacgcga
    33361 gtggtcagcg acaaaaagac catcgatatc tttgccgcca cccgcccgca ggcaaaatgg
    33421 ctgaaatgcg aactgggcga accgttgttt cgcatcagca aaatcgcctt tgatcagaat
    33481 gacaaaccgg tgcacgtctc cgaactcttc tgccgcgcca atcgcatcac cttaacgatt
    33541 gataataaaa gacattaacc gtaggccgga taagatgcgc cagcatcgca tccggcgatg
    33601 ctggcgcact gaattttaca tcccgcacgt tcccctcacc ctaaccctct ccccaaaggg
    33661 gcgaggggac cgttcagtgc ggaatttagc ttgattgccg aacggtgcta tccagcatta
    33721 aatactcgca atactctcac gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg
    33781 gttaatgcga atcgcacaat gttcgagttg tggattcgcc tggcgaaacg ttcctgaaag
    33841 tcgatagaag agcggcggga tttcatattt cgactctgca ccgaccgggt aaggcaacgc
    33901 gccacgcttt tgcgcttctt ccagcaccct ggcggcaatc agctgatgaa actcgacaat
    33961 caacaccttc gactgcgcat tggcaatgac ggcgcttttc acttccgcca ccgcgccgcc
    34021 gttaagcaaa gccagcaacc gttcggatac tcccgcctgc accgcatgca tcactggcgc
    34081 gaacaccaga ccacgcaaga cttccagcgc ctgtgagccc tggatctggc taccgccgga
    34141 gtaaagcgtg gcgcgaatcc gactaataac atcggcatcg ccaaccactg caccaacgcc
    34201 ctccggccca aacagcttga agcaggagaa tgtcgagaca ttcgcgccgc attcacagcc
    34261 gattcgcgct accttcatca ccgcatagtt atcatcggtt aacgctggaa cacctgccgc
    34321 gcgcaacgtt gccagcacat ctgccagcac atagctgtcc tgcggctgct ggcgcgtatg
    34381 ctgcaccagc gcggcatcag gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc
    34441 attgaaatca gcagtaataa gcgtcagccc catctgctca ataataaccc gtgtcgtcgg
    34501 gtaaacaggc gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag ccgccagccc
    34561 ggcgcgaatc gcgccagtcc ccgcgccctg caccagcgcc gcggcctgtg cgtgaaatgc
    34621 atcagccagc acctgctcca cccgttgggt aatacgcggt tgattcagac ctggagttaa
    34681 ccctaaatca ccgccagcaa gaaactcgct gcctggaaaa tggcggcaaa tagtgtccac
    34741 cagcgcaaac tgcttttgct gcgcttcagc aagagtcagg ctttgcaaag gaaacgtctt
    34801 cataggtgcc ttccttaagc cggaacgaac aggccaagcc agtaaagaac attcagcaga
    34861 ataccggtga tcattactgc cacgaccggt gccgccattt tctgtaccgg acggcccagg
    34921 gattcgttga ggaagtaaat ggcaaccgcg atagagaatc cggtataacc cgccatctta
    34981 attgctgcga aaatcgaacc gaccagcagc gccacttcca tcagcatatt catggcgtta
    35041 cggatgttat ccgacgcatt acgcaccgac gggtagcgtc ccagccattt gccgatcgaa
    35101 cgaagcagca agacttccgc cgaaataacc actgcgccta ataccgctgc aaccatcgga
    35161 ttcggcgaga gatagcccac cgcataaaca aaggtaaagc ccgcaactgc atacacaccc
    35221 gttgctaagg cggtggtggc aatcaacggc acaaacccca gtccgcgcat aaattctgcc
    35281 agagccgcct gattaatcag cgtttgcgat tgttccggcg tcacgcctgc ggaatacgct
    35341 ttctccagtg tgaagatcga aacttcgcta ccagcaaaaa tcttcatact ggcaacggcg
    35401 gcaatcaatg ctcccacgat ggcgatataa ggtaagtttt tgataatccg cgacgtgcgc
    35461 tcttcaaaca ccgaaagccc gctggcatcg atgtcatttt catcacgatg acgcaggtcg
    35521 tgagttatcg cgatccccag cagcatcacc atgccaataa agatttcgat ggattcaggg
    35581 ttaagatgtg ggaaatagcg cacgactacc acacgggtca tcagaacaac cacggcggcg
    35641 accagacttt gcttccagcc aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa
    35701 gcagaaacca ccggcgagct taattccccg aggctaccta atacatccac cggcagcgcg
    35761 gtcagcagct ggttaactgg caacaggcaa gtaaggatca acacgcccca gatagcgcca
    35821 agaccaaacg ccatcaggct gtttatcgcc agtacgccga ggatatcggt aggaagaaag
    35881 agtaaccatg cgttgagcag cccggttttc agggtaaaag agatccccac cgaggccaca
    35941 aaaccaatgc tcagaccaaa agcgatactc cccgcctcgc gacggttcat atagccttca
    36001 atcagttgcg gcaggatcgg gtggatgccg tcatgaaaaa cagccgccga gcgatgcgcc
    36061 agaagcgatg tcatacccgt caggcacgcc accacgataa tctgaatata cagatccata
    36121 gtcgtgcctt ttatttcaga tggttaatca acatcggaat cgcgtgttcg acgtgctcaa
    36181 cggaaaggcc aaacgccact ttcccttcag cgatcatttt ggcgatatgc tcgtctttcg
    36241 ctttaatgcc tggtttggca atggtgcaac ttttgttata gccaatcacc gcgatggcaa
    36301 tcgacaatgc cgcgcccgca ccggtattac acgcgccaat gtaataatct agttgcccgg
    36361 atttcacctt cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa cagccaggag
    36421 ccgtcgcttc aatggttttt ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt
    36481 tcatgagtct gtccttattg gaaaaattga gagggatttt cacgtaacat cacatccaca
    36541 tcggcctgac tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa taaatagtca
    36601 tagccataac caccattggc ttttaaatgg gagcggcgcg taatatccat cgacagcatg
    36661 acgcggttca gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg cttttcgtcc
    36721 gggtagtaac tgttcttacc aatggtgtcg aactgcacgt acgcgccgag atcgatcatc
    36781 ttcaaaatgt tgtcgaggtt gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga
    36841 tcaactccgt gggcttgcaa cagagccagt tgttccagcc ccatggtgct gaacgacgta
    36901 tgcgtggaga tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat aaataccttc
    36961 tcttccagcg gcgtaatctt tccttcgctg gagccgatct ccgcgatgat cccggctttc
    37021 agctccgtgc catcgatacc ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc
    37081 acgctgcggg tcaccacatg ttccgggaaa aacgcatcct ggtaataacc agtacaggcc
    37141 accacgttga tcccggtttc gcgcattaca tcgagcataa attgcgcatt gcgccccatg
    37201 taacggttgg tcatctcaat cacattacgt acaccccggg tcatcaggtc gttcatctcc
    37261 tggcaaatga acgcatactg atcaaggcgg cagtccacgt tgtttttaaa gccggagaga
    37321 tcaatatgca gatgctcatg ggccagggtg taacccgtcg gatcaaaact catagggtgt
    37381 ctcctgcaaa ccggagggag gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga
    37441 aaaattcaca cacggtagct cccacatcag aaagcgtggt gcgcacaccg agctgcgtgg
    37501 cgaccagccc ttgctgataa accagcactg gcaccacttc acgggtatgg tggctgtgac
    37561 caatggtcgg atcgttaccg tgatccgcca tcacgaccag gcaatcatct ggctgcatcg
    37621 cctcaacaag ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca taacgtgcga
    37681 cgtcttctgc atgaccagcg aggtcggttt cctggatatt gatgcaaata aacgccgtcg
    37741 gatgggtgtt aaattcgtcg agggtgatat ccataatccg ctggctatcc accaggtttt
    37801 gccagctcac accataagga ttgctgacga tatctgccac cttacccacc agcacggttg
    37861 gcacgcccgc ttcatacagt ttttgcggca cctgcacttt ttcatcaaca ccgtagccca
    37921 tatgcacgac ctggaagccg ttgtcataag cgccagaacg cggcgcatta ataccaataa
    37981 agcgcccttc tttgctttct gcggcatcga gaatacgctg actgttggtt aacaggccgc
    38041 caaatgtaat gacccgaccg acctgtacct ggtcacgcac gatacgacca attttgattg
    38101 cgtcgtcaaa agagatcaca gagagattgg cggtaatgtt atagacttgg cctaaatccg
    38161 cctcgagatt atcgccaatc gcaaccgcct gattgaccca cagaaattgc agatcatcgc
    38221 cacggcgctc cacctgccag ccagcggaaa ctaatgcctg ctcaacacgg tcaatcacat
    38281 cgcgaaaagg catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca
    38341 taaaggtatc gccaccttca tgttgcagct ctgccacgcc ccaggttgcg gaatctgacg
    38401 gctgcatatc gcctggcgca taacccaatg tgttgattag ccccagcttc tccagcgttg
    38461 gtagctgcaa atgcggcaac tggctcagga tgtgaccaca ggtattcgcc ccggcatctt
    38521 gcgggcgcac cagcgtgaca tctttcattg cccctacgcc aaagctatca atcactaaca
    38581 ccacaaatcg cgccatttat gcctccagag ggttgccgag gctgtcataa cgaccaacta
    38641 tttctggttc gccgcgctga ataccggaca ccagcaccac atcgctacgg gtgacaaaaa
    38701 tctgcgtgcg aaaacagagc accactgcac tgctcaccgg atactcgcct gccagcggca
    38761 gggtgtagtc gatactgctg tcatccacag ttttgagatt ggtttcagta atcttttgat
    38821 tttctggcgt aaacaccagc gcatgttgcg catgaccacg acggtagtaa ccgccgccgt
    38881 agcagtagct gtcgccacgg aaatgatggg agatttcact taaccagagc atcgcgatac
    38941 gttcaggctg atcgccctgc tggtttgccg gaatagtacc ggtcagcgca tgaccgggtt
    39001 cggcatgagt cgcaccgtat tccgccagca atggcagcga agtgcagctg gtcgctgaag
    39061 gtacgttcag ttgctcaatc gcaataccag atttcgccag ttgatcccgt gcttgtacca
    39121 gcgtgtgaag attcggtgtt ggcaaaactt tcccggcagc ctcatcccaa agcaggcaag
    39181 ggaaatgggt tagtccggct aaatgcagcc ccggcagttt cttaatttca gcaatcacct
    39241 cgtgcagcga atgctgtaca aaaccgctct cctgccccgg ataaagaaaa tcatcgtcgc
    39301 tataaacttt gagcagcaca ggctggcccc gtccggcctt aaccgctgcc gcagaaactt
    39361 cccgcgcttt gtcgagagta aacacggtga tgatgtcggt gccctgttca acggcgtcag
    39421 caacctgatg acaagggatt tgtaccagat gcccctgatg cgccactggc aaaccagcgc
    39481 ggcgcatgac tcgcgcctct ttgtaatcca ccgccacaat gccgctgtag cctaatgcca
    39541 gcaatttttc cgccagccac ggattacgac caaattgctt ggtcatcaga tacagttcaa
    39601 tgccgtaaag ccgcgccgtc tcaatgagcc gcttaccgtt ttccagtatc tgatccacgt
    39661 cgatcaccca gctgtccggg gcgattttgc cctgctgcca caaacttagt gcggcggaaa
    39721 tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat cttgttgcct cgtttctgtt
    39781 ccttgaatta aatattcatt tttttgaata tttaggttgt gcgacgtgga tttgtcggga
    39841 gcttcaaacc tcttcgttag cagccatcca taatccataa agattggcca gcaaatagcc
    39901 ttcttcacac gggttaactt ccagagcgaa ttccttcaac aacacctgat gcaattgcac
    39961 aacggcttgc cagtggctgg attgcgccag ttccgccagc aactcgttat ccagcggctc
    40021 tatttcttca ccacggcgac tgcgcatcag tgcactcgcc atatgtgtca tcgccatcgt
    40081 tccttgctca ctgcgcaccg gcagatggca ctctttttcc agtacgttga cgacctgcat
    40141 catgcctttg cagacgtcct tatcaataac gcctgcctcg caaagcaggt tgagtctggt
    40201 ttccatatca gatgcctttc gtttaataac tgggttcctg ttccccactc accacccgtt
    40261 gttgatgggc aagcagggcg tgtttatcta caacggcacg cagcaattgt tgcattgggt
    40321 aatcatcgac tcgcgtcagg accacggctt cggtggcctg taaaaatcgc ggatcgtctg
    40381 ttagcggcgt cgcctctaat cccagcatgg tcagttcgtt ttccgccacc acgttccaga
    40441 tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact ctcgtgataa gagagatcga
    40501 ctcgttccac atcactatcg ccaaaaaaga catcggtcat gattttctga tccgccgaac
    40561 gactatcgag acctactcgc ttaacatttc cagactcacc tttgcggcat atcagttgat
    40621 gttcaccaac atacgtatgc gggccaagct cgagggcgat gcacaaattg ttttggctga
    40681 gataactttc cgccgccagg cgggaaacta ctgccatgtc atagacaccg ttaagtaaac
    40741 actccacgcg aatatccgcg ccgcgcatat gcgcatagta aaaaggaatg ccatcaaact
    40801 gggctttcaa tccgctcgct aaaccttcgt acaagcgggt atagggcaaa ggcatcgcac
    40861 ataccacgtt gttgatatcc acatgactca gcaatgcttt gttatccatc tcgacaagat
    40921 aactgccatt gcgcccacgg cgttcaatcc gtatcgctcc gcttgattcc agcgttttca
    40981 acgcggcctg cgtcaggcca accgatgaac ggcattcagt tgccagctca tctatggttt
    41041 tcagtcgatt accgcatttt tcacccaaca aataacgggc cagtgtcgtg atgacgacgc
    41101 cttctttttt gataaacgtt cgacgcataa tatattttca ataaaatgaa tatttatatc
    41161 ttcattaaaa tgaagataag tggcaacgac gagatgtgga aacggcgagg cgcttcacat
    41221 tttttcgggt tgttgcgaaa agaggataaa aaaaccgggt ttccccggtt tcagagtgat
    41281 gacgaagtga tttattcgac aatgcgagca aagggtaatt cccactggcg cttgcctgct
    41341 cccctcaccc taaccctctc cccaaagggg cgaggggacc gtccgagcgt tttgatatta
    41401 cggcttcgcc acaaaaccaa tcgcttcgta caccgctttc agcgtacggg aagcgtgtac
    41461 actggctttt tctgcgccgt ctttcatcac ctgttgcagg aaggcttcat cgttgcggaa
    41521 acggtgatag cgttcctgca attcagtcag cataccggaa acggcatcag ccacttcacc
    41581 tttcagatga ccatacatct tgccttcgaa ctgtttttcc agttctggga tgctctggcc
    41641 cgttaccgca gaaaggatat ccagcaggtt ggaaacgccc gctttgttct gcacatcgta
    41701 gcgaactacc ggcggctcgt cggagtcagt gaccgcgcgt ttgattttct tcactaccga
    41761 tttcggatct tccagcaggc cgataacgtt attgcggtta tcgtcagact tggacatctt
    41821 cttggtcggc tccagcagcg acattacgcg cgcgccagat ttcggaataa acggctccgg
    41881 caccttaaag atctcgccat acagcgcgtt gaaacgctgg gcgatatcgc gactcagttc
    41941 caggtgctgt ttctggtctt cacccaccgg taccagatta gtttgataca gcaggatgtc
    42001 cgcagccatc agcaccggat agtcaaacag accagcgttg atgttctcgg cataacgcgc
    42061 agatttatct ttaaactgcg tcatgcgact cagttcgccg aagtaggtat agcagttcag
    42121 tgcccagcct aactgcgcat gttctggtac gtgggactga acaaaaatgg tgcttttctc
    42181 aggatcgata ccacaagcca gatacaaggc cagcgtatcc aacgtcgctt tacgcagctt
    42241 ctgtgcatcc tggcgcacgg tgatcgcgtg ttggtcaacg atacagtaaa tgcaatggta
    42301 gtcatcctgc atgtttaccc actgacgcag cgcacccatg tagttaccaa tggtcaattc
    42361 acctgagggc tgtgcgccac taaaaacgat gggcttagtc atttttcgat tcctgatttt
    42421 cgctatgcgg aagccctaat gcgggcagaa ggtcatttat agactggtaa attacatcag
    42481 gctggctgag atcgatagcc tcgccgtagt tatatccgta ggttaagcca actgatgggc
    42541 aacctgccgc ttttgccgcc tgaatatcat tgcgtgagtc gccgacaaac agcatctgtt
    42601 gtggggcaat tcccatccgc tcagccacca gtaacagcgg gtccggatgc ggttttttgt
    42661 tttgcacatc atcgccgccg ataaccacgc taaagtattt ggcaatatct aaggcttcga
    42721 gcagcggcgc gacgaacggc gtcggtttgt tggtgaccag gcctagcggc aggcctttag
    42781 cctgcaacgc gcccaacgta tcggcaacgt gcgggaacaa aaacgtcccc tcttcggcaa
    42841 cctcgccata gtagcgatcg aacagtttac gcagaatacg tacctgttct tctgccggaa
    42901 tgtcgtcatc aacgggcggt ttccccattg ttttacgcag agtcgcacgt tcctgacgcg
    42961 cccaggtcaa tgcgcgctcc atcagaacat ctgcgccgtt accaatccag gtaataacgc
    43021 gttcttcacc tgcgacgggc aactccagcg catacagcgc catatctacc gcagcagcaa
    43081 gaccaggagc actgtcgacc agcgtaccat caagatcaaa agcgacgccg cgaatatctt
    43141 caaacttatt catgacttac ctttgccagt tcactgcgca tttcatcaat gactttttta
    43201 tagtctggct ggtcgaagat tgccgaaccg gcgacgaaca tatccgcgcc cgccgcagcg
    43261 atttcgccaa tgttgttcac cttcacgcca ccgtccactt ccagacgaat gtcaaagcca
    43321 gactcgtcga tacggcgacg tacttcgcgc agtttatcca gtgtttgagg aatgaaagac
    43381 tgaccgccga aaccagggtt gacggacatc agcaggatca catccagctt atccatcacg
    43441 taatccagat agctcagagg tgtcgccggg ttaaatacca gacccgcttt acagccattt
    43501 tctttaatca gttgcagcgt gcggtcaaca tgctcggagg cttctggatg aaaggtaatg
    43561 atgctggcac cagcggcagc gaaatcaggc acaatgcgat cgacgggttt caccatcaga
    43621 tgtacgtcga taggggcggt aatgccgtag ttacgcaagg atttcagcac cattggccca
    43681 atcgtcagat tgggaacata gtggttatcc atgacgtcaa aatgcacgac atcagcgcca
    43741 gctgccaggg cttttgcggt atcttcaccc aggcgggcaa aatcagccga cagaattgag
    43801 ggggcaatca aatactgttt catccgcttc tccttgagaa ttattttttc gcgggtgaaa
    43861 cgactcctgg tttgtacaaa gccagcagtt cgtccacctt tttacgtgtg ccgccgttgc
    43921 tgcttatact gcgtcgaact ttgacgacat gcaattttgc gcgctgatac cactcacgcg
    43981 ttaacatcgt atcgtgattg gagatcagca ctggaatatg gcgctcaacc agaccttcgg
    44041 cgatctccgc cagatgcgct tgttgttcaa gcgtaaaact gtttgtgtga tacgccgtaa
    44101 agttggcggt cgcagacagc ggtgcataag gcggatcgca atagacgacg gatgcatcat
    44161 ctgcgcgcgc catgctatcg gcgtaagact cacaatagaa aaaggcattc tgcgcttttt
    44221 cagcgaagtg atacaactct gcttccggga aatagggttt tttgtagcgg ccaaacggca
    44281 cgttaaactc accgcgcaga ttgtaacgac acaggccgtt gtaaccgtag cggttcaaat
    44341 ataaaaacag taccgcccga cggaacggat cctggctttt gttgaactct tcgcggaact
    44401 gatagtaaac ctcggcgcaa tttgtttcgg gaacaaacag ctcgcgtgcg gcctgtacgt
    44461 actcatcagt acgcatcttc acaatgttat agagactgat caggtcgcta ttgatatcgg
    44521 caaggatata acgagaaaag tcggtgttga gaaacaccga cccggcacct acaaaaggct
    44581 caaccagaca ttcgcccttg ggcaaatgcc gtttaatatc atcaagcagg ggatacttgc
    44641 cccctgccca cttcaaaaaa gcgcgatttt tcttcatgct gactaactaa ttacaccttc
    44701 tccggctgtg gagaaagctc cgacagcatc cagcgcttca ggcagctccc gcaacgataa
    44761 accattgcgg gagataacct tgattacttc agatcgacct gtacctgacg cagcggtttc
    44821 gcccacgggt ttttggcctg aacatctgct ggcaatgtag ataccgcttt tttcgcctct
    44881 tctttcgaag cgtacacgcc agaaaccagg acataccacg gctgaccatt acgcgtcgtt
    44941 tcatagacaa cgtagttttt cagattctct ttcttcgccc aaccgttcag gttgtcgtag
    45001 ttagaggaac tgctcagctg cagagtgtaa tggctggacg gtgccgattt caacgaacca
    45061 acattacctg cggtcttccc tccagcggct ggtgttgccg tggtttgcgc cggggatgcc
    45121 gtctgtactg gagccgtagt cgccgtctct tttggtgctg gcgtagaagt cgcagccggt
    45181 gccttcgtgc tcgccacagg ggcagctagt tcagtacgct tcggcgtctg tgctaccggc
    45241 ttcggctccg ttttcacggt tgcttgcggt tttttcggtt caatcaccgc ctgctgacga
    45301 actgggcgcg tagtggacgg acgttcagcg gtttgcgttt tcgccgtgtc acgcgatgca
    45361 ttgccattgc gaacaggcgc aaccgttgcg ggttcagtcg gcaacgtgga attgaccgcc
    45421 acattgttca gctgttgctg attttgtggc tgggtcagcg cattgttcag gtcaccctgc
    45481 acttcaacac gttgttgacc atccgttgcc accggggttt gcccttgagt cggcgtagaa
    45541 gagatcggtg gcagagaaac atcctgctga gtattttccg cagacgtggt tcccggcgct
    45601 ggctgcacgc cattcgcctg atcggtcgca ttgccagcaa gatcaatact cttctcgcca
    45661 gacgcggttt gatcgctgga agtggtcgag ggggctttta gcgcagaacc gataccgatg
    45721 atcaacagca gtagaaccag aatgccgacg cccatcatca tatactgacg agaagcgggt
    45781 ttactggctg cttttttgcg cttacgcgga cgacgctcta cgcgctcttc atccacggtt
    45841 tcatcttcgg attcatcaat ttcttcttcg atttccggtt cctcattgcg ctctttttgc
    45901 gcacgagtcg gacggcgatc gtcagtgtca tcaagttcaa tatcatcaaa attgatctgc
    45961 ggttcgccac gttcagtacg ctcagaacgt tcagaagatt gacgagaacg accagtacga
    46021 cgatcgctgg gatcgggttt cagctcgtct tctggtttga attcatccat ttaacacccc
    46081 actaaaaggt taatgcttac cacgttgcaa ttaacctgaa gctaatagac cgcttgataa
    46141 gcggcctgac ctttcttgtt gttacgctga ttgacaatcg gcaatggcgt taagaacaag
    46201 ctcgtgcgaa acgccgctgc gaacttcact cttaccaatt gccaacggaa gaattaagcg
    46261 catctctccc gcaaggactt tcttgtcacg cagcatatgc ggtaaatacg cctgcgcgga
    46321 cattttgcgc ggcccattga ccggtaaccc agcccgcgtg agcagggtta taatacgctg
    46381 cgtttcggca gaactaaact gcccgagacg ttccgacgtc cgcgccgcca tcaccatacc
    46441 cgcagcgacc gcttcaccat gtaaccaatt gccatacccc atttcagctt caatggcatg
    46501 accaaaggtg tgtcccagat tcagtaaagc acgtaacccg gtttcgcgct cgtcggcggc
    46561 gacaacttct gccttcagtt cacaacaacg gcgaatacag tacgccattg ccggaccgtc
    46621 cagacgcaac aacgcatcca gattctcttc cagccagtta aaaaacgcac cgtcaagaat
    46681 aatgccgtat ttgatgactt ctgccagccc cgacgctaac tcacgcgggg gaagcgtttt
    46741 cagacagtcg agatccacca ccactgaagc aggctggtag aacgcgccaa tcatgttttt
    46801 accgagggga tggttgaccg cagttttgcc gccaacggag gaatcgacct gcgacagtaa
    46861 cgtcgtcggg acttgaatga aacgaacacc gcgctgataa ctcgccgccg cgaagccggt
    46921 cagatcgccc actacgccgc cgccaagcgc caccagcgta gtatcgcgac catgcggctt
    46981 ttgtaacaac gccgtaaaga cggtatcgag tacagccagg cttttatact gctcgccgtc
    47041 agggaggata acgctatcga cgttaacacc cgcctgttca agtacgccgc ggaccttatc
    47101 gagatacaga ggagccaggg tttcgttggt gaccaacatg acctgctcgc ccgatttcag
    47161 cggtaagaat gaagctggtt cattaaacaa accagatgcg atggtaattg ggtaactacg
    47221 ttccccgaga gtaacgacaa tcctctccat aacgcgacat ccaccttaat tactgtaccc
    47281 gcagacgagt gtatataaag ccagaattag ttgctttcca gcatgtgaat aatctggttt
    47341 gcaaccactt tagcgctttg atcatcagta cgaatggtca cgtcggcaat ctcttcatac
    47401 agcggattgc gttcattggc caacgcttcc agaacttcac gcggcggtgt ttcaacgtgc
    47461 agcaacgggc gttttttatc acgctgcgtg cgtgcaagtt gcttttcgat ggtcgtttca
    47521 agataaacga caacgccacg agcggaaaga cggttacgcg tttcacggga tttcacagag
    47581 ccgccgccag tagccagcac aataccctgt ttctcggtca actcattgat gaccttttct
    47641 tcgcgatcgc ggaagccttc ttcgccttct aaatcgaaaa cccagcccac atcagctccg
    47701 gttcgtttct caatctcttg atcggaatcg taaaattcca tattgagttg ttgagctaac
    47761 tggcgcccaa tagtgctttt tccggcaccc ataggcccaa ccagaaagat attgcgtttc
    47821 tctgccattt tttcggtact actaagacta ttcgttaatg ataaacccgc ttcgctcaga
    47881 gagcgccgca ggacatgaac tgaaacctca taagatattg cgagagtcag actgaaaatt
    47941 atctcaatac tccagcgggt ttggcaactg aataaatcac ccatccctct gcatatctgg
    48001 tcgctgcaag cgcgctgcct tgctaccacc gctctggcga taaatcaccg ggtaagatta
    48061 gcgtaaaaaa gacagcaaaa tgccgcctga atgataaatc atcatcatgg aacgcaggca
    48121 gcgaagagca ccaactctca aatcggtact ccttgtatgc taaatacctg cgcgcgtcaa
    48181 atagatgaaa cacgttcagc ataaaaacat taccgctttt acggctgttt actcactgga
    48241 aaccagtcgt ggcgtgataa acaccactaa ctcgcgtcgt tcatcttctt ttccgtcatg
    48301 acgaaataat tgcccgaacc agggaatgtc gccaagcaac ggtacgc
//
LOCUS       DADLSE010000013        47485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLSE010000013 DADLSE010000000
VERSION     DADLSE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.013"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN692_000770"
     CDS             complement(33..1220)
                     /gene="eutG"
                     /locus_tag="JN692_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HAY7263158.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQLRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSERKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDSD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN692_000771"
     CDS             complement(1210..2046)
                     /gene="eutJ"
                     /locus_tag="JN692_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HAY7263159.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVM
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2057..3460)
                     /locus_tag="JN692_000772"
     CDS             complement(2057..3460)
                     /locus_tag="JN692_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HAY7263160.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECRSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN692_000773"
     CDS             complement(3472..3759)
                     /gene="eutN"
                     /locus_tag="JN692_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HAY7263161.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN692_000774"
     CDS             complement(3866..4159)
                     /gene="eutM"
                     /locus_tag="JN692_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HAY7263162.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN692_000775"
     CDS             complement(4198..5214)
                     /gene="pta"
                     /locus_tag="JN692_000775"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7263163.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLAHAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGYKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRTLTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN692_000776"
     CDS             complement(5211..6014)
                     /gene="eutT"
                     /locus_tag="JN692_000776"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HAY7263164.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQLVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN692_000777"
     CDS             complement(6011..6712)
                     /gene="eutQ"
                     /locus_tag="JN692_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HAY7263165.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN692_000778"
     CDS             complement(6687..7166)
                     /gene="eutP"
                     /locus_tag="JN692_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7263166.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN692_000779"
     CDS             complement(7179..7514)
                     /gene="eutS"
                     /locus_tag="JN692_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7263167.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN692_000780"
     CDS             complement(7807..10086)
                     /gene="maeB"
                     /locus_tag="JN692_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7263168.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10375..11325
                     /gene="tal"
                     /locus_tag="JN692_000781"
                     /pseudo
     CDS             10375..11325
                     /gene="tal"
                     /locus_tag="JN692_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
     gene            11345..13348
                     /gene="tkt"
                     /locus_tag="JN692_000782"
     CDS             11345..13348
                     /gene="tkt"
                     /locus_tag="JN692_000782"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7263169.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     NPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(13451..14494)
                     /locus_tag="JN692_000783"
     CDS             complement(13451..14494)
                     /locus_tag="JN692_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7263170.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSSELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN692_000784"
     CDS             complement(14620..15195)
                     /gene="nudK"
                     /locus_tag="JN692_000784"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7263171.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN692_000785"
     CDS             complement(15263..17242)
                     /gene="aegA"
                     /locus_tag="JN692_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7263172.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17448..19160
                     /gene="narQ"
                     /locus_tag="JN692_000786"
     CDS             17448..19160
                     /gene="narQ"
                     /locus_tag="JN692_000786"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HAY7263173.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGKPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAISCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEGKGNT
                     PAEV"
     gene            19362..22475
                     /gene="acrD"
                     /locus_tag="JN692_000787"
     CDS             19362..22475
                     /gene="acrD"
                     /locus_tag="JN692_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7263174.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGID
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN692_000788"
     CDS             complement(22574..22633)
                     /gene="ypfM"
                     /locus_tag="JN692_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7263175.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            23014..23370
                     /locus_tag="JN692_000789"
     CDS             23014..23370
                     /locus_tag="JN692_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7263176.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23374..24501
                     /gene="dapE"
                     /locus_tag="JN692_000790"
     CDS             23374..24501
                     /gene="dapE"
                     /locus_tag="JN692_000790"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7263177.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            24529..24729
                     /locus_tag="JN692_000791"
     CDS             24529..24729
                     /locus_tag="JN692_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7263178.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN692_000792"
     CDS             complement(24839..25537)
                     /gene="ypfH"
                     /locus_tag="JN692_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7263179.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(25611..27626)
                     /locus_tag="JN692_000793"
     CDS             complement(25611..27626)
                     /locus_tag="JN692_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7263180.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     LRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(27641..28504)
                     /locus_tag="JN692_000794"
     CDS             complement(27641..28504)
                     /locus_tag="JN692_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7263181.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNHLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(28672..29385)
                     /gene="purC"
                     /locus_tag="JN692_000795"
     CDS             complement(28672..29385)
                     /gene="purC"
                     /locus_tag="JN692_000795"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7263182.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(29598..30632)
                     /gene="bamC"
                     /locus_tag="JN692_000796"
     CDS             complement(29598..30632)
                     /gene="bamC"
                     /locus_tag="JN692_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7263183.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(30649..31527)
                     /gene="dapA"
                     /locus_tag="JN692_000797"
     CDS             complement(30649..31527)
                     /gene="dapA"
                     /locus_tag="JN692_000797"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7263184.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAACDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            31673..32245
                     /locus_tag="JN692_000798"
     CDS             31673..32245
                     /locus_tag="JN692_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7263185.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKSTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            32245..32715
                     /gene="bcp"
                     /locus_tag="JN692_000799"
     CDS             32245..32715
                     /gene="bcp"
                     /locus_tag="JN692_000799"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7263186.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            32968..33585
                     /gene="hyfA"
                     /locus_tag="JN692_000800"
     CDS             32968..33585
                     /gene="hyfA"
                     /locus_tag="JN692_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7263187.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            33585..35602
                     /gene="hyfB"
                     /locus_tag="JN692_000801"
                     /pseudo
     CDS             33585..35602
                     /gene="hyfB"
                     /locus_tag="JN692_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            35613..36560
                     /gene="hyfC"
                     /locus_tag="JN692_000802"
     CDS             35613..36560
                     /gene="hyfC"
                     /locus_tag="JN692_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7263188.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            36577..38016
                     /locus_tag="JN692_000803"
     CDS             36577..38016
                     /locus_tag="JN692_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7263189.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLLGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            38028..38678
                     /gene="hyfE"
                     /locus_tag="JN692_000804"
     CDS             38028..38678
                     /gene="hyfE"
                     /locus_tag="JN692_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7263190.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            38683..40263
                     /gene="hyfF"
                     /locus_tag="JN692_000805"
     CDS             38683..40263
                     /gene="hyfF"
                     /locus_tag="JN692_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7263191.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     ARGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGILKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            40253..41967
                     /gene="hyfG"
                     /locus_tag="JN692_000806"
                     /pseudo
     CDS             40253..41967
                     /gene="hyfG"
                     /locus_tag="JN692_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            41977..42528
                     /gene="hyfH"
                     /locus_tag="JN692_000807"
     CDS             41977..42528
                     /gene="hyfH"
                     /locus_tag="JN692_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7263192.1"
                     /translation="MLKLLKTIMRAGTTTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTV
                     THKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCP
                     ECKQRATLINDDTDVPLVAKEQL"
     gene            42525..43283
                     /gene="hyfI"
                     /locus_tag="JN692_000808"
     CDS             42525..43283
                     /gene="hyfI"
                     /locus_tag="JN692_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7263193.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            43276..43689
                     /gene="hyfJ"
                     /locus_tag="JN692_000809"
                     /pseudo
     CDS             43276..43689
                     /gene="hyfJ"
                     /locus_tag="JN692_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
     gene            43719..45755
                     /locus_tag="JN692_000810"
     CDS             43719..45755
                     /locus_tag="JN692_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HAY7263194.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEELLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCTAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVRRCCNLLLQEYA"
     gene            45752..46600
                     /locus_tag="JN692_000811"
     CDS             45752..46600
                     /locus_tag="JN692_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HAY7263195.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAILHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(46638..>47485)
                     /locus_tag="JN692_000812"
     CDS             complement(46638..>47485)
                     /locus_tag="JN692_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7263196.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
BASE COUNT        10608 a        12938 c        12439 g        11500 t
ORIGIN      
        1 ggataagacg cggcaagcgt cgcatccggc atttattgcg ccgctgcgta caggccgaca
       61 atctgctcca ggctggcggt acgcgggtta ctgcgcagac aaatatcttc cagcgcggcc
      121 tgcgcccatg cgccgtaatg cgcagatgtc gcaccaacat cgcccagtcg tttaccaatc
      181 ccaacttcag caatcagctc acttaccgcg ttaatagcgt cactatcgtc ggatttttta
      241 gttcgcagcg cccgaccaat ctgactaaac cgatcacgac aaaccatccg gttaaactcc
      301 atcaccgttg gcagcaacat agcgttcgcg agaccatgtg gaatatgcag cgccgcgccc
      361 ggctgatgcg ccatcgcgtg gcacaaccca agacccgcac tggaaaacgc cattcccgcc
      421 atacatgaag ccagcaacat gctctcgcgc gcggcaaggt cgtgaccgta gcccaccgct
      481 ttcggcagcg atttgccaat catcgcaatg gcaccaatcg ccagactgtc ggtaaacggt
      541 gtagcgttca gggcgctgta tgcttcaatg gcatgggtta acgcatcaat gccggtcatc
      601 gccgtgacat gcgacggcac accttcggtc aatgcggcgt cgaggatcgc cacatccggc
      661 atcagcgagg catgggctaa cacctgcttg cgctcgctca ccgcgtcgat aatcaccgtt
      721 acattggtgg tttcagagcc ggttccggcg gtagttggaa tggcaatcag cggcaagcgc
      781 agttgcagaa cgctggtttc tgacatctct gccagcgtgc tatccgggtt cgtcaccagc
      841 aacgccacgg ctttcgccgc atccagcacc gagccgccgc caaatgcgat caccccgtca
      901 caacctgact cacgcaactg cgccacggct gcacacacgt cggtaatgca cggttcgccc
      961 accggacatg gccagagcgt catggcgata cctttaacgg ccaggctacg cgtcagcccg
     1021 gcggtcatcc ctgcctgatg caaaaagctg tctgccatca cgaacagatg tttcagccca
     1081 cgcgtttgcg cttgctgtcc gcaactgctc accgagcccg gaccgcaaag cgtcaccggt
     1141 ggaacgctaa atgtttttac ccgttgcaga ttcagggtat cgaacgcctg aaagagcgcg
     1201 gtctgcaatt cattttgcat atagcccctc cgctttctct cgcccactac tggcgatcgc
     1261 cagcggggtc ataaacaaac tgtgctgcgg taaatgcacc tgtaacgccg ggaattgttt
     1321 gcgaaacagt tctgccacgc ccggttgcat acaggagccg cccgccagcc ataaatcagt
     1381 aatgccctgc ccttcaatat gccgggcgac aatgtccgcc attttttcgt acaccggttt
     1441 caccgcaggc caaatctctt caccgtgacc gcgcttgtac tgctccgcct cttccagcga
     1501 aatacggcga tttccggcga gagtcagaga gatgtgatgt ccgccggtgg cttcatccgc
     1561 cgagtacatc actttgccct ttttcacgat ggcaatgccg gtagtgccac cgccgatatc
     1621 cactacaccg gcgttatcca gttgcagtag atccgcgact gcggttggct catccagcac
     1681 atggctcact tccagcccgg cagattccag cacgttgatg gaaatacgcg ggtccgtacc
     1741 tggaggaaat gaagtcgccg catggctaaa acgacgaccg aattgctgct cgagcgtgtc
     1801 gagatggcga cgaacaatgg tgaccgcgcc gaagaaatcc cagacgatgc cgtcgcggac
     1861 cacatcggcc cagtcgaggc ataccgccac cggctgaccg tcgcggtcga caaccatcga
     1921 caccacatcg caggtgccca gatccacccc cagccacagc ggggattccg tcgcggcagg
     1981 cgtctggtta cacagcgttg ccgccgtttg cagacgtggg gtgagccatt gttcgtcgtg
     2041 cgccatcggt tactccttaa acaatgcgaa acgcatcgac taatacacag cgacgcagac
     2101 ggacaaacgt acgcgcgctg gttacccctt caccggttgg cgtggtgatg gtcatggtgg
     2161 tccagccttc cccgcccagt cccagcccgg caatgcacgg tccgttctta acgaaaatgc
     2221 tggtatcaat ggcattcgcc atctggttca tgttctcgat attgcgcgag tgcattgccg
     2281 ccgtgtggtg gcaaccgccc tccagtttca ccgccagcgc aatggcatcc gccacgttgg
     2341 cgacgcgcac gacgggcagc accggcatca tcagttcggt cacggcaaac ggatgttctg
     2401 cagtggtttc cacaaacagc aaacgcgttt cttgcggcac gttcaggccg attgccgccg
     2461 cgattttcgc cgcatcgcga ccaacccagt cacggctgac ggtgcctttt ccgcgctcgt
     2521 cgatattttt cagcaacacc ggttgcagct gctgcgcctg ttctgcggtc agtttcaccg
     2581 cgtgctggcc ttccatcaga cgcatcagtt catcggctac gctatcaaca acaatcagta
     2641 ccttttcgtc ggcacaaatg atgttgttat cgaaagacgc gcctttgacg atggactgag
     2701 cagcacgggc gaggtcagcg gtttcatcca ccactaccgg cgggttgcca gcgcctgcgg
     2761 caatcagacg tttattggtg tgtttacgcg ccgcttctac taccgcttcg ccgccggtca
     2821 ccaccagcag gccgataccc ggaaacttga acaagcgttg cgcggtttcg atatccggat
     2881 ttgccacagt aaccagtaag ttttccggcc cacctgcggc aacaatcgcc tggttgagca
     2941 gcgtaatcgc ccgctgggag acttttttcg ccgccggatg cggggcaaaa atgacgctgt
     3001 tgcccgcggc aatcaggctg atggcgttgt taattacggt tgccgccggg ttagtggaag
     3061 gcgtcaccga agccaccacg ccccagggtg cgttttcaat tagggtcagg ccgttgtcgc
     3121 cagtcagcac ttgcggagag cggcactcaa cgcctggtgt gccgcgcgcc tgagcgacgt
     3181 tttttgcaaa tttatcttca acgcgcccca tgccggtttc actgacggca agttccgcta
     3241 aatctctggc gtgtttttcg cctgcttcac gaatggcagc aatggctaac tggcgcattg
     3301 ccacgctttt taacccttgc tgggcgactt tggctgccgc aacggcgtca tccagggacg
     3361 cgaaaacgcc catctcatga acagcggcgg gcggcgtgtc actgctttgc attttcagca
     3421 gtaccgcttt caccacctgt tcaatatcct gttgattcat gatgttctgc cttatttgtg
     3481 gaaaattacc tgaccgccag acaccacctc atcgacgatg ccaatcacgc acagatcgac
     3541 cggtgacgtt tcgcttttat gcgcctggcg ggcggaactg ccactcacca gcaacaccca
     3601 ctccccggtt cccgcgccaa tattgtcgat ggcgacggca cattgcccgt caggattacc
     3661 ttgtggatca atcatttcca ccatcagcaa tttgtcatgc gccagtccgt gatggcgtac
     3721 ggtacaaaca atttgtccag tgacgactgc cagtttcata cccgcctccg tggcgtattt
     3781 caggtaaaag ctccccctac cctccgcaga aggtaaaatg aaaaaggaga gagcgtgacg
     3841 cccgaatcga cgtcacacag ggtgattaca ggttgctgct atcgcctttc aggccgatcg
     3901 ggaacacttc ttccaggtcg ccgtgcggac gcgggataac gtgtacagat accagctcgc
     3961 cgatacgctg tgccgctgcg gcaccggcat cggtcgccgc tttacaggct gcaacatcac
     4021 cacgaaccat ggcagtacac aggccgccgc caatctgctt aacaccaacc agcttgacgc
     4081 gcgcagcttt caccatcgcg tcagaagcct caatcagtgc aaccaggccc cgggtttcga
     4141 tcattcctaa tgcttccatt gtgctttcct ctttatcagg gtccagaacg ggaccgttca
     4201 ttcaaccagt gtttgtaaac tgctttcgcg gttcatttct gtctgacgcg gcacagctgc
     4261 caccagcgcc agctcgataa tttcctgcac gctacaacca cgagagagat cgtgcatcgg
     4321 cgcggcaagt ccttgtatca atggcccgac ggcacgatat ccgccgagtc gttgtgcgat
     4381 tttgtagcca atatttccgg cttccagcga cggaaaaacc atcacattgg ctttgccctg
     4441 tagcgggctg gcaggcgctt tttgcgccgc cacttccggc acgaaggcgg cgtcaaactg
     4501 caactcgcca tcaaccacca gctttggtgc gcgctcacgg acgatttctg tcgcctgctg
     4561 gacgttggca acgcaggggt gacgggcgct accgttactg gaaaacgaca gcatcgccac
     4621 gcgcggctct tctccggtga gggtgcgcca ggtttcggca ctggcaagcg cgatatccgc
     4681 cagttgcgcc gccgtcggct gtggcaccac gctgcaatcg gcaaagccca acgccggacc
     4741 gctgtactgt ggcagcatca ggaaaatgga tgagagcgtt ttatagcctg gctgcaagcc
     4801 gataatgcgt aatccggcac gcagcacatt cgccgtggaa gagaggttgc ccgcgataca
     4861 gacatccgct ttaccggcgc tgaccattgc tgcggcgaac atcagcggat cggtaagttt
     4921 ttccagcgca tccggcggcg ttttttcgcc cgcgtgggcc agccagcgat gagcaaattc
     4981 ttcccgcatt gcgaggttgc catgcggatc aatcacctgt agcccgtcca tcgccacgcc
     5041 gtgactgagt gcaaactgac gaagttcaaa cggattagcg accagaatgg gcgttgccag
     5101 accttgttga tgtaaatatt gcgcagcttt cagcacacgt tgatctaacg catccggaaa
     5161 aaccactctg gcgggcgctc gcaacgccag ttcacgacaa cgttcaataa tcattgcgtt
     5221 tcccccagtc gttgttggat ctgcttcacc gttagcggcg gcttcgttac gctcaggatc
     5281 atcattacgt agaccgtgct cgaaaggcgg ttcagcgcct gcaagatgtc cgggcgtaat
     5341 acttcaaaac tgcgggtgat aaacacctgc gccgctacgg tttcggtttc acgcactttg
     5401 gtgcgtagca gattgagcaa cgccgcatcg cgtccgtggc tggcttccgg caccagatga
     5461 tcgtgaccaa gataacgcaa cggctgatga gaaagccggt gcagatcttc gtcagaaagc
     5521 ccgacaatcg cctggcagcc cagcggttcc cccagcgcat cggcgcgcat aatgttgcca
     5581 agacgcgaac ggatatccgc cagccacggc tgccacggtt ccgccagttc aatttgcagc
     5641 cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa aacccagacg cggatcgctt
     5701 ttggcgacca ttttttccgc cgacaggtga gtcagcgtat ccggcttttt tgccaccagc
     5761 tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc tactagtcag cccatgaacg
     5821 ggctgcggct gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc aataaacttg
     5881 atgcgcagat ggcggctttc cagcaactcc cgggcagagg gcgtcaggcg actgtccgca
     5941 gggagatgga tctctgcgcc ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg
     6001 atgaaatctt tcatagggat tgccagttag ccggccaggc gacatacagg aatttcacgc
     6061 tggatgtcgt accaaattcg atgctggagc ctttcgggat aaacatcacg tcgcctgctt
     6121 tggcgatcat ggtttcgcct tcgtggcgaa catgcagctc cccttccagc accatgtcga
     6181 tttcgtcgta gttcagggtc cacgggaaga atgcgttctc ccactgcata aaaccggcgg
     6241 ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa gcccacgcaa tgcggctctg
     6301 cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat cactttgatg ccgcctttgc
     6361 cggtcaccga tttgaagctc ggctgcattg cgccctgctc cagcgactgt ttttccttca
     6421 tcactttttc catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg
     6481 caatgatggt ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca
     6541 cggacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg
     6601 ccacttcacg ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt
     6661 cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc
     6721 tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg
     6781 ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg
     6841 gcgacgtcag catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg
     6901 ccaatatcca gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag
     6961 atcagcatgt ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta
     7021 aaatattcac ccggcgtatc aatatcgccc ttatcattaa attccacggc ctgtgttttt
     7081 ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg
     7141 ctacccacaa acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa
     7201 agcgtataat ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact
     7261 gcgcccaccg agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc
     7321 acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc
     7381 gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgcaag ttcctcaccg
     7441 ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg
     7501 cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg
     7561 tcagagtaaa taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat cactaagaat
     7621 ggagagatat ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat
     7681 aatatcgaga tattttatat actgattttt ttattattcg caaactcagt aataagcttt
     7741 gctaatatat ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag
     7801 aatgaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat
     7861 acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac
     7921 cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc
     7981 agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat
     8041 acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat
     8101 cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg
     8161 gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa
     8221 acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc
     8281 gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcgttcat
     8341 tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca cgctaaaatg
     8401 ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat
     8461 gatcgcgccg atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac
     8521 accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga
     8581 ttcgttattg acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat
     8641 gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac
     8701 cagttcctga gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt
     8761 cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa
     8821 ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga
     8881 ctccatcgcc gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg
     8941 ttttggaatg atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac
     9001 cacttcactc tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat
     9061 ctcttcgttg atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca
     9121 aagaacgttg ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg
     9181 acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat
     9241 cattggtgcc cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca
     9301 gcccaggaaa atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac
     9361 caccgcatat gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc
     9421 ttttgaatcg caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat
     9481 acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt
     9541 ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg
     9601 atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca
     9661 ttctggcgct ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac
     9721 gacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc
     9781 agcgaatttc ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc
     9841 aatgttgcct aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg
     9901 cgcggtatat ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac
     9961 gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac
    10021 ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc
    10081 atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc
    10141 tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca
    10201 aactctcacc attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt
    10261 aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttcgcg aaatttctca
    10321 gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac
    10381 gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga tattgagtcc
    10441 attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact caaggctgcc
    10501 ggattatcac aatatgagca tttaatagac gatgctatcg cctagggtaa aaaaaatggc
    10561 aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct
    10621 gaaattctca aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt
    10681 gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc
    10741 gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc
    10801 gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttgcccag
    10861 gcgcgggcct gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat
    10921 gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt
    10981 aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg
    11041 ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact
    11101 atcgcaccga atttgttgaa ggagctgcag gaaaaagttt cgccagtggt acgtaaatta
    11161 atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg
    11221 gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct
    11281 gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg
    11341 ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgcggtaca
    11401 aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct
    11461 gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt
    11521 tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta
    11581 cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca
    11641 cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt
    11701 ggcgaacgcc gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc
    11761 agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga
    11821 aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactgggca agctgattgg
    11881 tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga
    11941 tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacaa
    12001 tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct
    12061 gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc
    12121 gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca
    12181 ccatccgcca tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg
    12241 cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca
    12301 actggcagaa gagtttaccc gacggatgag cggtggttta ccgaaggact gggagaaaac
    12361 gactcagaaa tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc
    12421 ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc
    12481 ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc
    12541 ggcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg
    12601 catcgcgcac cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata
    12661 cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac
    12721 ccacgactca attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc
    12781 cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg tggaagcggc
    12841 ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag
    12901 gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg
    12961 ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga
    13021 gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt
    13081 agtttccctg ccctcgaccg atattttcga cgcccaagat gaagaatatc gggagtcggt
    13141 gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta
    13201 caagtatgtt ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc
    13261 ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca
    13321 taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca
    13381 ccctaaccct ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct
    13441 gcgattttgt ttaacgggtg atccacaacg tgggccaggc gtctggccca tgccagttat
    13501 cgcaggtggg ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc
    13561 gcgcctgaat gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcactcg
    13621 atttctcatc aaatgttgcg ttcatcagtt ccagttcatt cgtctcctga ccgttgttga
    13681 acggcaaacg caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg
    13741 ccaaatcaat ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat
    13801 cggtcagcgc agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat
    13861 tcatccgcca gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg
    13921 gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg
    13981 gttcgtcccc tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa
    14041 taaacaacaa cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg
    14101 tgatcgcctt cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg
    14161 ttactaataa ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat
    14221 ctaatagcag ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc
    14281 gctcaatacg taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca
    14341 gtccattatg atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct
    14401 gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag
    14461 cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa
    14521 caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga
    14581 ttgagccaat aataaacaac gaaatattat tgttattttt cagtccatta aatgtgacat
    14641 ttgcaaatag ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat
    14701 ctccagcgcc tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc
    14761 accggcgtta gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt
    14821 cacaccgcca ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc
    14881 cgtctcttca atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc
    14941 ggcgcaggtt tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa
    15001 ctgacgaatc agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt
    15061 accgcgatcg taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc
    15121 gtaagtaatg ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt
    15181 gatttgttgc gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag
    15241 tcgggtttgt ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa
    15301 taatcccctg tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac
    15361 gcacggcgtc gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt
    15421 ccacatccgc gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg
    15481 gcatcccgtg tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt
    15541 cactgccctc aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg
    15601 tgcgcaggaa acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct
    15661 ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc
    15721 ctggcatgtt agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca
    15781 gcgcagtacg cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc
    15841 cggcggtatt gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt
    15901 tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg
    15961 ctttcatgga acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg
    16021 aatccaaaga gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc
    16081 tgaagatttc ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga
    16141 aagtgagcaa gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc
    16201 gggtcagaac atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct
    16261 tgtccacttt ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg
    16321 aaatatagcg ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg
    16381 caccttcaca caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg
    16441 tctggtgaga aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact
    16501 gcggaatatg gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa
    16561 ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa
    16621 aaccggtttt gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct
    16681 gcatttgttc gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg
    16741 gttgatgctc ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga
    16801 cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt
    16861 cgcgccctgc acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg
    16921 gtgtcaggac gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa
    16981 tacacttttg ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc
    17041 agctacgggc gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct
    17101 gatgtttgat aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat
    17161 cattgtgagc catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt
    17221 tggccataat aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt
    17281 atatgagtgt tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat
    17341 aagtaactat ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc
    17401 gtttcagcga actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac
    17461 gacccgtctc ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt
    17521 ccacgggtat cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata
    17581 ttgccggatc gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc
    17641 cacaactcaa tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca
    17701 acctcaacgt ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca
    17761 actggctgga gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata
    17821 ttaataatta tgttaatcag atagacctgt tcgtgctggc tttacagcac tatgctgaac
    17881 gcaaaatgct gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg
    17941 tcttttttac tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta
    18001 ccgccagtca gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc
    18061 cgaatgagct tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat
    18121 tgtacctttc gctggaggcg tcagtagaag aaaagacccg cgatctccac gaggccaagc
    18181 gtcgtctgga ggtgttgtat cagtgttccc aggcgctgaa caccagccag attgatgtgc
    18241 attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa tatctggagc
    18301 tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag ctgccaatgc
    18361 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    18421 acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt
    18481 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    18541 tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt atccagttga
    18601 cgttactgaa gcgttcgata ccggaagata acgccactgc acaaagtatc atggccgatt
    18661 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    18721 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    18781 atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    18841 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    18901 ccaacgccag cgaaatcgcc atcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    18961 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggac
    19021 tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt tcacaacctt
    19081 ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggggag gggaagggga
    19141 acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt taaaggcgag
    19201 agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc atgataattt
    19261 acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt
    19321 caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg
    19381 atcgccccat ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggt accctggcga
    19441 ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg
    19501 ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc
    19561 aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt ggcaccggtc
    19621 aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag
    19681 tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg
    19741 tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg
    19801 gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa
    19861 gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct
    19921 ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca
    19981 ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata
    20041 agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc
    20101 gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca
    20161 ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct
    20221 ccgggttggg ggtaaaactg gcctccggcg caaacgaaat ggcgacagcg gagctggtgc
    20281 tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt
    20341 atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag
    20401 ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga
    20461 tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg
    20521 gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg
    20581 atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc
    20641 ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga
    20701 tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct
    20761 atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga
    20821 tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt gagcatcacg
    20881 ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg
    20941 aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc
    21001 tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag
    21061 accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc
    21121 tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt
    21181 cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt
    21241 ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta
    21301 tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc
    21361 caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg
    21421 ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg ctggcggcgg
    21481 aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga
    21541 ttgatatcga ccagcgtaaa gctcaggcgc tgggcattga tatcgacgat attaacgaca
    21601 cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga
    21661 agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct
    21721 ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct
    21781 gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg
    21841 gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga
    21901 agcagctgcc aaacggcttt ggtctggagt ggacagcgat gtcgtatcag gagcggcttt
    21961 ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg
    22021 cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg
    22081 taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg
    22141 tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg
    22201 ctaacgagat gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc
    22261 agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg
    22321 caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg
    22381 ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc
    22441 gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat
    22501 gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttatac
    22561 tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt
    22621 cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg
    22681 tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta
    22741 aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc
    22801 atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata
    22861 ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat ttgaatactt
    22921 cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc cttaaatgag
    22981 tatttattct cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat
    23041 tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga ctatcgtttt
    23101 catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat caacgaatta
    23161 ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaactacc
    23221 cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat gcctgcaatt
    23281 atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgattcc
    23341 agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca
    23401 caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca ggctttgttg
    23461 attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc cgatacgcag
    23521 aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca taccgacgtg
    23581 gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac cattcgtgac
    23641 ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta
    23701 gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc atttctgatc
    23761 acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga ggcgttaatg
    23821 gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat cgaagtggta
    23881 ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac cattcatggc
    23941 gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct
    24001 ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt cccggcgacc
    24061 agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat tccgggtgaa
    24121 ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat gatcaaagcg
    24181 caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc
    24241 gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag
    24301 cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt
    24361 attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac tattcataaa
    24421 attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc
    24481 atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat ggactggctg
    24541 gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt
    24601 aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg
    24661 catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg
    24721 aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg catccggcaa
    24781 tggtgaacga tgcctgatgc gacgctcacg cgtcttatca ggccaggtaa tctgtctttt
    24841 acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct tcgtcgaaat
    24901 aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg cgattgtcga
    24961 tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca ctgattaacg
    25021 cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca ccatgaatca
    25081 agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt ccgttaaagg
    25141 caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc ataatcgcgc
    25201 cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt ttctgccagt
    25261 agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg cgattatctt
    25321 ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc ggctccgcgc
    25381 caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg cctatttccc
    25441 ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt tgtgcaggtt
    25501 tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta actgatgtca
    25561 ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag tcagtgaaaa
    25621 aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt aagtgcgaac
    25681 agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc tgaaagttta
    25741 agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg cagcgccggt
    25801 aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt taatagcgga
    25861 cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag gatggcatcg
    25921 tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg cgcatcgcga
    25981 cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat cggtaacagc
    26041 gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg caccagcaca
    26101 aaaccgcagc gttgccagaa acgccataac tccccggtgt aaccaaaact caccgaaaga
    26161 tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgccgccc tgtgccttcc
    26221 cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa tgtcgccgcc
    26281 agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg cgggcgacga
    26341 aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc caccagccac
    26401 aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc tggtgcatcc
    26461 atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa taactgataa
    26521 acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc ggaaatgacg
    26581 atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc ctcactaacc
    26641 attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc aaaacggtgt
    26701 aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc ttcgtagccc
    26761 tgcaccgtag tggttaacaa cgttcgagga aaacgcaata ccagttgatg caacaacggc
    26821 gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg cttgctcatc gctggctaac
    26881 aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa attgtgccag tacatccgtt
    26941 gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa tacgagaaat gagttgccct
    27001 gccaacgccg acttaccgcg cccacgcgca gccgttaccg ctgccacgcc cggcggcatg
    27061 gtcagtagct gctgtaagag ttgctgttgt tctggttgtg gtgcgccggt cgcggggtgc
    27121 cagtcagtac ggggagtaaa atgcgccaac gagaacggct ggttttgccg ccagaggata
    27181 gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga caaaatgcgg cgtcgcaata
    27241 gggtcagggc aatcactcca gcgcagcgag tcggcatcag gttggttttc ccactcttcc
    27301 catacaggga gtaacaaaac cagccagctt cccgctttca acgttccgct aagtgctgca
    27361 aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg catgccggaa ctcgcgccca
    27421 agtaaagttt gtagcgccga gggtgaacag tggttttcag catctagctg cggcgaaatc
    27481 cacagccagt cgccaggtaa ggcatcacgc aacttaagag catgatcaaa acaccaaccc
    27541 tcttccccgc tcaacaccag caagcggcag atcccttcac gtttcatttg cgctgttaat
    27601 gtgtaaagcg cagtcagttc agccatccct gccccgaaaa ttaaatgctt ttaccaaaag
    27661 tattgcattg tgccggatcg ccgctgtcga aaccacgttt aaaccagctg tagcgttgct
    27721 gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc ctgactttgc tgttgtaaac
    27781 ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc cagatcgcca gtttccagaa
    27841 cgccttgctg ctgcatacta tgcccccaga caccggcaaa acagtcggcc tggagttcca
    27901 tacgcacaga taagtggttt acttccgcct gcgtcgcgtt ttgttgcagt tgacgaactt
    27961 tcggctcgat gcctaacagt ttctgcacat gatgaccgac ttcatgggcg ataacgtacc
    28021 cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat gtcatcatag aaggagagat
    28081 cgatataaac tgtgccatcc gccgggcaat agaacggccc cataatggac tggcccgccc
    28141 cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt cggttgctga taggtcttac
    28201 ccatcttttc gaactgttgt ccccaggtgt cttccgtggt tgccagaatc accgaggtga
    28261 attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt tgattgttgt tgagaaaccg
    28321 gctgcccggt catcaacccg gttaaatcaa cgccatagta gcctgcaacc agcacgacta
    28381 tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa accgggaccg cccattgacg
    28441 gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact ttcacgtcgc ccttgccaac
    28501 gcatagatac ctcaacaata tattcattat agaaatgatc gtaggtggtt cagaggaaga
    28561 ttaccacagg aaagcgggat gaaatggcgg aggaatgagc gttcaatgca tatattccga
    28621 gagcggttgt cttgccgtgc cagctgcacg gcaagatgat gaacagaaaa atcagtccag
    28681 ctgtacaccc aggcggcggg cgacggcttc ataggcttcg atcaggccac cgaggctctg
    28741 gcggaaacgg tctttgtcca ttttctccag tgtttctttg tcccacaggc ggctgccgtc
    28801 cggggagaac tcatcgccca gtaccacttc acctttgtac agaccaaact ccagcttgaa
    28861 gtcgaccaga atcagaccag catcatcgaa cagttttttc agcacgtcgt tcgctttgta
    28921 ggtcagctct ttcatacgcg ccaggttctc tttgctcacc cagccaaagg tttcgcagta
    28981 agattcgttg accatcggat cgtgcatggc atcgtttttc aggaacagat cgaacagcgg
    29041 cgggttcagc tcaatacctt cttcgattcc aagacgtttc accagagagc cagcagcacg
    29101 gttacgcacg acacactcaa ccggcaccat atccagcttt ttcaccagac attcggtatc
    29161 ggagagcaga cgctccattt gagtcgggat acccgcttca gccagtttgc tcataatgaa
    29221 gtagttgaac ttgttgttca ccataccttt gcgatcaaac tgctcaatgc gcgcgccatc
    29281 ccctgctgac gtatcattgc ggaattcgag caccaacagg tccgggtttt ccgtgctgta
    29341 tacggttttc gctttaccac gatacaactc agcttgcttt tgcatcttta tcactcctgg
    29401 gtgtgaatta acgttttaaa atcttttgct gtctggtgtg ccggatgttt tgtcggatgc
    29461 ggcgtgaacg ccttatccga actacgtccg acaaacaaat ttcgtgcgaa ttaccgctaa
    29521 aatcgcgttt tcctgccgac gcacacgttt gcgtattata tcagaaaaaa gggccggatg
    29581 attccagccc tgtattttta cttgctaaac gcagcctgga agacagctac cagcgcgtcg
    29641 ttctgactct gagtcagagt atgacctttc ggatcgatga actgtaggct gctgcggtta
    29701 tctaaatcgc caacctgcag tttatagtca ccggatgcca ggcctggatc gctcgcgccc
    29761 agttcctgcc agtcgctgtc agacagcggc ttataagtta cggccatgtt gccctgcgaa
    29821 cgggtgctgt cggtcacttt catgcccact ttttccagcg ccgctggcag acgttgccaa
    29881 accacattga acggcccgcg tacgaccagc attggtaaac cggtgtcatc agctgcactt
    29941 tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt tcgcggcgtc agtggcagat
    30001 ttatccagac cggcggaaat aacgttcatc atctccgtgc tgtaacgctg catggaagcc
    30061 gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca gtttaaccgt aaccgcctgc
    30121 tgataaccct gcggcttaac agagatttga taacgaccac gatactgctc gtcttcgtcc
    30181 agacggttcc attgtaccca atcggtggtc agtgtctgac cagcatcatc acgttgggtg
    30241 atggtgtagt ttttcgcctg cagcacgcta accacctgcg gccacagagt attgccacga
    30301 ccattttcca ccagcaatga agcggtatcg cccgtgaact gggtacgcgc gccagaaacc
    30361 agtgccagcg gctgggctgg tggacgaatg tccagcgcct taccgacagc accactaccg
    30421 ttggtcaccg ggattgcata atcaccggag gtcaccggca aaatcattcc agccggggca
    30481 tgaagctccg caagcggtgc cgcttccagg taggcttcat caccactgac ctgacgctta
    30541 tagcgtgagt cagaactaca ggcagcgagt aataaaacaa gcgaaacacc cgcaaccttt
    30601 gccaggcgcg acttttgaac agagtaagcc atcaaatctc cctaaacttt acagcaaacc
    30661 ggcatgctta agcgccgctc tgaccgtctc acgaccactg tcggtgattg gtgtcattgg
    30721 caggcgcagc gtatcggtcg ccacaagacc cagttcctta catgcccatt tcaccgggat
    30781 tggattgggt tcgacaaata gtttgttgtg taatggcatc agacgctgat taataacgcg
    30841 tgcctcggca aaatgccctt ctgctgccag tttgcacatc tgggccatat cacacgctgc
    30901 gacgttagcc gtaacggaaa taaccccatg accgcccaat tgcatgaagt ccagcgcgct
    30961 cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc agctctttga tctggtttac
    31021 acgcgttaag ttccctgttg cctctttgat tccgataata ttttttactt tcgccagacg
    31081 gcccaccgtt tccgggagca gatcgcagcc agtacgggac ggcacattat acagaatttg
    31141 cggcaggtca gtatgctcag cgatggcttt gaaatgctga tacaaacctt cttgcgacgg
    31201 acgattgtag taaggagtta ccgtcaggca gccgacgata ccactgtcat tgaagcgctg
    31261 cgtcaggcta atggcttccg cagtagcgtt agcgccggtc ccggcaatta ccggaatgcg
    31321 cccatcagcc agatccagcg tcatcatcac cacatcagca tgttcgtcat gatttaaggt
    31381 agcggactcg ccagtggtgc caacagaaac gatcgccgaa gtaccgctgg cgacatgata
    31441 atcaatcagt tttttcaagc tagcccgaca gacattacct ttttcatcca tcggagtaac
    31501 aatcgcgaca atacttcccg tgaacatggg ccatcctctg tgcaaacaag tgtctcaatg
    31561 gtacgtttgg tatggcatta aaagcaagca gacagaaccg ttctgattgt tgtatgcatg
    31621 atttttttat gctttcctta agaacaactc accccttaaa ggaataacca gtttgacact
    31681 gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc gatcgccctg gaattgtgaa
    31741 caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt gaagacagtc gcctggcgat
    31801 gctgggagaa gagttcacgt ttatcatgct gctttccggt tcatggaatg ccatcactct
    31861 gattgaatca acgttaccgt tgaaaggtgc cgaactggat cttttaatcg tgatgaagag
    31921 cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc tgggttcagg tcgatgttgc
    31981 agactccccg catttaattg aacgcttcac agcacttttc gacgcgcatc atatgaacat
    32041 tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa cgggctgcgc agttgcatat
    32101 tcagataacc gcccacagcc ccgcatctgc ggacgcagca aatattgagc aagcgttcaa
    32161 agccctatgt acagaactca atgcacaagg cagtattaac gtcgtcaatt attcccaaca
    32221 tgatgaacag gatggagtta agtaatgaat ccactgaaag ccggtgatat cgcaccgaaa
    32281 tttagcttgc cggatcaaga cggagaacaa gttaatttga ccgacttcca gggacagcgt
    32341 gttctggttt atttctaccc gaaagccatg acccccggct gtaccgtaca ggcctgcggc
    32401 ttacgcgata acatggatga gttgaaaaaa gcgggcgttg atgtgctggg tatcagcacc
    32461 gataaacccg aaaaactctc ccgttttgcg gaaaaagagc tgcttaactt tacgctcctg
    32521 tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct ggggtgaaaa gtccttcatg
    32581 ggcaaaacct acgatggtat tcatcgcatc agcttcctga ttgacgctga tggcaaaatc
    32641 gaacatgtct ttgacgattt caaaaccagc aatcaccacg acgttgtgct gaactggctg
    32701 aaagaacacg cctgattact ttgctccatt ccgtgctggc tgcgcttgcg gccagcatac
    32761 ctcacttctc gtgatcaaga tcacattctc gctttcccct gcgacacggg tgtcgaatcc
    32821 attttttgct gaacgttaat gaccatcatt tttgtaccgt tcagaatcca gttaatacat
    32881 aacttattga atagattgag ttaatcagaa tggcatcctt tatgcaatat gaaatgcaat
    32941 gtttcatatc attttcaagg agccgacatg aaccgctttg tggtggccga accactgtgg
    33001 tgtacaggat gtaatacctg tctcgctgcc tgttcggacg tgcataaaac gcaaggttta
    33061 cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa tcactgcccc tgtcgtgtgt
    33121 catcactgtg aggaagcccc ttgcctgcag gtctgcccgg tcaatgccat ctctcagagg
    33181 gatgatgcga tccaactcaa cgaaagcctc tgtattggct gcaagctttg cgccgtggtc
    33241 tgcccatttg gcgcaatcag cgcttcagga agccgtccgg tgaatgccca tgcgcaatat
    33301 gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa acgtgccgcc ccaacatgcg
    33361 ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga aatgcgacct gtgtgatttc
    33421 ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc aggcgttacg gctgatcacc
    33481 gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc ttgccgcaag ctggtttgcc
    33541 aatggcgggg aggatcccct ttccctcact caggagcaac actaatggat gccctgcaat
    33601 tattaacctg gtcgctgatc ctctatctgt ttgccagtct ggcttcgctg tttttactcg
    33661 gtctggacag actggctatt aagctttccg gcatcacatc gctggtgtgc ggcgtgattg
    33721 gcatcatcag cggaattacg caattacatg ctggtgtaac tttagtcgcc cattttgcca
    33781 ccccttttga ctttgccgat ttaaccctgc gaatggatag cctctcggca tttatggtgc
    33841 tggttatctc cttgctggtg gtggtttgtt cgctctattc attgacttat atgcgcgaat
    33901 acgagggcaa aggcgcggcg gcgatgggct tctttatgaa tcttttcatc gcatcgatgg
    33961 ttgccctgct ggtgatggac aacgcttttt ggttcatcgt gctgtttgaa atgatgtcgc
    34021 tgtcttcctg gtttctggtc attgccaggc aggataaaac gtcgatcaac gctggcatgc
    34081 tctacttttt tatcgcccac gccggatcgg tgctgattat gatcgccttc ttgctgatgg
    34141 ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg ctttcacttt ctccggggct
    34201 ggcgtcggcg gtgtttctgc tggccttttt cggttttgcc gcgaaagccg ggatgatgcc
    34261 gttgcacagc tggttgccgc gcgctcaccc tgccgcacca tcgcacgctt cggcgttgat
    34321 gtctggcgta atggtcaaaa tcggtatttt cggcatcctg aaagtagcga tggatctgct
    34381 ggcgcaaacg ggtttgcctc tgttgtgggg cattctggtg atggcgatcg gcgcaatctc
    34441 cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat atcaaacggc tgctggcctg
    34501 gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc ggtgtggcga tggacggtct
    34561 gtcactgcac gacccgctgc tcaccgtggt tggactgctc ggcgcactgt ttcatctgct
    34621 caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg ggcgcgatta tttcgcgttt
    34681 gcatacccac gacatggaaa aaatgggggc actggcgaaa cggatgccgt ggacagccgc
    34741 agcatgcctg attggttgcc tcgcgatatc agccattcct ccgctgaatg gttttatcag
    34801 cgaatggtac acctggcagt cgctgttctc actaagtcgt gtggaagccg tagcgctaca
    34861 acttgcgggt cctattgctc tggtaatgct ggcagtcact ggtgggctgg cagtaatgtg
    34921 cttcgtcaaa atgtacggta ttactttctg tggtgcgccg cgcagtacac acgctgaaga
    34981 ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta ctgctcgcgg cactctgcgt
    35041 attaattgcg cttagtgcca gttggctggc accgaagata atgcatattg cccatgcgtt
    35101 taccaatacc cctcccgtca ctgtcgccag cggaatagca cttgtacccg gcacgtttca
    35161 tacacgggtc actccttcat tactgttgct gttactactg gcgatgcctt tgctgcctgg
    35221 cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt cgtcgcacag gagatgcctg
    35281 ggcatgcggc tacggctgag aaaacgcgat ggctccgtca ggcaatggcg tgatgcagcc
    35341 gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa cagctcgacc ctacgctgag
    35401 gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag agcacagaac ccttctggga
    35461 tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg ctggtcaaag aaatacagca
    35521 tctgcaaagc ggcgactttc gtctctattg cctgtatgtg gtcgccgcac tggttgtgct
    35581 gctaatcgct attgccgtct aaggaaatca ccatgagaca aactctttgc gacggatatc
    35641 tggtcatttt tgcgttagca caggccgtga ttctgctgat gctaacccca ctttttacgg
    35701 gtatttcccg gcagatacgc gcgcgtatgc actcccgccg cgggccgggg atctggcagg
    35761 attatcgcga tatccacaaa ctgtttaaac gccaggaagt tgcgccgaca tcttcaggtc
    35821 tgatgttccg cctgatgccg tgggtattaa tcagcagcat gctggtgctg gcgatggcct
    35881 taccactatt tattaccgtt tccccttttg cgggcggcgg cgatctgatc acccttatct
    35941 atcttcttgc cctgtttcgt tttttctttg ctctttccgg gctggatacc ggaagtccgt
    36001 ttgcgggagt cggtgccagt cgcgagttga cgctcggcat tctggtcgaa ccaatgctta
    36061 ttctctcact gctggtattg gcgctgatag caggttccac gcatatcgag atgatcagca
    36121 atacgctggc gatgggctgg aactcgccgc taaccaccgt actggcgtta ctggcctgtg
    36181 gttttgcctg cttcattgag atgggaaaaa ttccctttga tgttgctgaa gcagaacagg
    36241 aattacagga aggcccgctg accgaatatt ccggtgccgg gctggcgcta gcgaaatggg
    36301 ggctggggct gaaacaggtc gtgatggcag cactgtttgt ggccctgttt ctgccctttg
    36361 ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc acttgtcgtt acgctgctca
    36421 aggttttgct gatttttgta ctggcctcaa tcgcagaaaa cacgctggca cgcgggcgtt
    36481 ttttactcat tcaccatgtg acctggcttg gcttcagcct tgctgcgctt gcatgggtct
    36541 tctggttaac cggtctgtaa ggagcactga cggaatatgg aaaatcttgc tctgacgacg
    36601 ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt cgccacaacg tcgggccgcc
    36661 gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca tgttgtcgct gatctccgcg
    36721 ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg tcaacgtggg ggatgtggca
    36781 ttgtttggcc tggtcattga tcgcgtgagt acgctgattc tgtttgtggt ggtgtttctc
    36841 ggtttgctgg tcacgatcta ctccacgggt tatctgacgg ataaaaatcg cgaacacccg
    36901 cataacggca cgaatcgtta ttacgcattt ttgctggtgt ttatcggcgc gatggcggga
    36961 ctggtactct cctcgacgct gctcggtcag ttgttgtttt ttgaaattac gggcggctgc
    37021 tcctgggcgt tgatcagtta ttaccagagc gataaagcgc agcgttcagc actaaaagcg
    37081 ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg ccgccgccac gctgtttttg
    37141 cagaccggaa cgtttgtgct tagcgcgatg agcgagttac atggcgacgc acgttatctg
    37201 gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg cccagctacc gatgcaagcg
    37261 tggctaccgg acgcaatgga agcgccaaca ccgatcagcg cctatctcca cgccgcatcg
    37321 atggtgaaag tgggcgttta catttttgcc cgcgccatta tcgacggcgg caatatcccg
    37381 catgtgattg gcggcgttgg catggtcatg gcactggtca ccattcttta tggctttctg
    37441 atgtatttgc cacagcagga tatgaagcgg ttgctggcct ggtcgaccat cactcaactt
    37501 ggctggatgt tcttcggctt gtcgctctcc atcttcggct cgcggctggc gctggagggt
    37561 agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc tgtttttcct tgtagcaggt
    37621 gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc tgcgtggcgt attgcacacc
    37681 ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg caattaccgg tgtgccgccg
    37741 ttcaacggct tcttcagtaa attcccgctg tttgccgccg gttttgcgtt gtcagtggag
    37801 tactggatcc tgctgcccgc catgattctg ctgatgattg aatcggtcgc cagtttcgcc
    37861 tggtttattc gctggttcgg tcgcgtcgtg ccgggcaaac cgagcgaggc cgtcgccgat
    37921 gccgcaccgc tgctagggtc aatgcgcctg gtgttgattg tactgattgt gatgtcgctg
    37981 atttccagcg taatcgccgc gacctggttg cagtaaggag atgatgaatg accggttcta
    38041 tgatcgtaaa taatctggcg ggactgatga tgctgacatc gctgtttgtg attagcgtca
    38101 aaagctatcg cctgtcatgc ggattttacg cctgccagtc gctggtgctg gtgtctattt
    38161 tcgccactct ctcgtgcctg ttcgccgcag agcaactgct gatctggtcc gccagcgcct
    38221 ttatcaccaa agtgctgctg gtaccgttaa tcatgactta cgctgcacga aatattcccc
    38281 agaacatccc ggaaaaagcg ttattcggtc cggcaatgat ggcactgctc gcggcgttaa
    38341 ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct accgatggct accgggctga
    38401 aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg cctgctgtgc attgtcagcc
    38461 agcgcaatat cctgcggcaa atttttggtt actgcctgat ggaaaacggc tcccatctgg
    38521 tgctggcgct tcttgcctgg cgagcaccgg aactggtgga aataggtatc gctaccgacg
    38581 ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa aatatggcgt acccacggca
    38641 cgctggacgt gaacaacttg accgcgctga agggataatg agatgagtta ttctgtgatg
    38701 ttcgctttac tcctgctcac gccgctgctt ttttcgctgc tctgttttgc ctgccggaaa
    38761 cgggggctct ctgcgacttg tacggtgact gtattacata gcttagggat cacactgctg
    38821 ctgattctgg cactctgggt ggtccaaact gccgctgatg caggagaaat attcgctgcg
    38881 ggactgtggc ttcatattga tggtctgggc ggtttgttcc tcgccattct tggtgtgatt
    38941 ggctttctca ccggtgttta ctcgattggc tacatgcgtc atgaagtggc acgcggcgag
    39001 ctttcacccg ttacactgtg cgattactac ggtttcttcc atctgttttt gttcaccatg
    39061 ctgctggttg ttaccagcaa taacctgatt gtgatgtggg cggcgatcga agccaccacc
    39121 ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt catcgctgga atcagcatgg
    39181 aagtacatca ttatttgtac tgttggtgtc gcttttggtc tgttcggtac cgtgctggta
    39241 tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg cgatattctg gagcgaggtt
    39301 cttaagcaat cgtccttgct tgacccaaca ttaatgctgt tggcctttgt gtttgtgcta
    39361 attggctttg gtaccaaaac cgggctattt cccatgcacg cctggctgcc ggatgctcac
    39421 agtgaagcgc cgagtccggt cagcgccctg ctctccgccg tattgctgaa ctgcgcgctg
    39481 ttggtgctga ttcgctatta catcattatt tgccaggcca tcggcagcga tttccccaac
    39541 cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg cggcattttt cattctggta
    39601 cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg agaacatggg gctggtcgcg
    39661 gtggcgctag gcattggcgg gccgctggga atttttgccg cgctgctgca caccttaaac
    39721 cacagtctgg caaaaacgct gctgttctgc ggttccggca atgtactgct caagtacggc
    39781 acgcgcgatc tcaacgtcgt ctgtgggata ctcaaaatca tgccatttac cgccgtgctg
    39841 tttggcggcg gtgcgctggc gctggcaggg atgccgccct tcaacatttt tcttagcgaa
    39901 tttatgaccg ttaccgcagg actggcacgt aatcacctgc tgattatcgt cctgctgtta
    39961 ttgctgttaa cgctggtgct ggcgggcctg gtacggatgg ctgcgcgggt gttaatggcg
    40021 aaaccgccgc aggccgttaa ccggggtgat ctcggctggt tgaccacctc gccaatggtg
    40081 attctgctgg tcatgatgct ggcgatggga acgcatattc cacaacctgt catcaggatc
    40141 ctggcgggcg cttccactat agtcctctca gggacgcacg acctgcctgc acaacgtagc
    40201 acctggcatg attttttgcc ttcaggcacc gcatctgttt cggagaaaca cagtgaacgt
    40261 taattcatcg tcaaatcgtg gcgaagcgat tctcgccgcc ctgaaaacgc agttccccgg
    40321 cgcggtgctg gatgaagagc gacaaacgcc tgaacaggtc accattacgg tgaaaatcaa
    40381 tctgctgcct gacgttgtac attatcttta ttatcaacat gatggctggc ttccagtcct
    40441 gtttggcaac gacgagcgga cacttaacgg tcattacgcg gtttattatg ccctttcaat
    40501 ggaaggggcc gaaaaatgct ggatcgtggt gaaggcactg gtcgatgccg acagtcggga
    40561 gtttccgtca gtcacaccgc gcgtccctgc cgcggtctgg ggcgagcgag aaattcgcga
    40621 tatgtacggg ctgattccgg ttggcctgcc ggatcagcgt cgcctggtgt tgcccgatga
    40681 ctggccggaa gatatgcatc cgctgcgcaa agatgcgatg gattatcgac tgcgccctga
    40741 accgacgaca gattccgaaa cgtatgcgtt tatcaatgag ggcaacagcg atgcgcgggt
    40801 gatccctgtc ggcccgctgc atatcacctc ctatgaaccg ggtcacttcc gcttgtttgt
    40861 ggatggcgag caaattgtcg atgctgatta ccgcctgttt tatgtccatc gcggcatgga
    40921 gaaactggca gaaacgcgga tgggctacaa cgaagtgacc ttcttatcgg accgcgtgtg
    40981 tgggatttgc ggttttgccc acagtgtggc ctataccaac tcggttgaaa atgcactggg
    41041 gattgaggtg ccgcaacgag cacatactat tcgctcgatt ctgctggaag tcgagcggct
    41101 acacagtcat ttgctcaacc ttggcctctc ctgccatttt gttggttttg ataccggctt
    41161 tatgcaattt ttccgcgtgc gggaaaagtc gatgacgatg gcggaattgc tgaccgggtc
    41221 gcgtaaaacc tacggtctga atctgattgg tggtgttcgc cgcgatattc tcaaagagca
    41281 acgtctgcaa acgctgaaac tagtgcgcga gatgcgcgcc gacgtgtcgg agctggtaga
    41341 gatgctgctt gctacgccga atatggaaca acgcactcag ggcattggca ttctcgaccg
    41401 acaaatcgcc cgtgattata gccctgtagg gccgctgatc cgcgcagtgg ttttgcccgt
    41461 gatttgcgct ttgatcaccc ctacgccgac tacggcaata ttccaaaaac actgtttacc
    41521 tttaccggcg gcgatgtttt ctcccgcgtg atggtccgtg tcaaagagac gtttgattcg
    41581 ctggcaatgc tggaatttgc cctcgacaac atgccggata ccccactgct gaccgaaggc
    41641 tttagctata aacctcacgc attcgcgctg ggctttgttg aagcgccacg cggtgaagac
    41701 gtgcactgga gcatgctcgg tgataaccaa aaattgttcc gctggcgctg tcgtgccgcc
    41761 acctacgcca actggccggt gttgcgttac atgctgcgcg gcaataccgt ttctgacgca
    41821 ccgctgatta tcggtagcct tgatccctgc tactcctgta ccgaccgtgt gacgctggta
    41881 gatgtgcgca agcgccagtc aaaaaccgtg ccgtataaag agatcgaacg ctacggcatt
    41941 gatcgtaacc gttcgccgct gaagtaagga cagaagatgc tgaagttact gaaaactatt
    42001 atgcgcgccg gaaccacgac ggtgaaatat cccttcgcgc cactggaggt cagcccaggc
    42061 tttcgcggga aaccggacct gatgcccagc caatgtattg cctgcggtgc ctgcgcctgt
    42121 gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca aaattcgcgc
    42181 acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga agtgtgcccg
    42241 accagagcca tccagcttac caataacttt gaactgaccg tcacccataa agccgatctc
    42301 tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc gtttgccccg
    42361 caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc gccacaaaac
    42421 cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg cgcgacgctg
    42481 atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt ccagtgctta
    42541 cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg aagcggcatt
    42601 tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc ggctgcaacg
    42661 cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa cgttttggca
    42721 ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc gcagtcaccc
    42781 gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat aaaatctgtg
    42841 tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac agcgtctggg
    42901 gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg ccaacaccgg
    42961 ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag attcacgcgg
    43021 tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag atcccgccat
    43081 cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt caggggcgag
    43141 aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat cgggttaaca
    43201 cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa ctctttcgcg
    43261 tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac gctgcgaaaa
    43321 aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat gtattactcg
    43381 ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt ccgctgccca
    43441 ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg acgtaagatg
    43501 ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc cctgttgacc
    43561 cgggcattcg cgccactggc agatgacgcg acgtctgtgt gacaagcgcg tagcattcaa
    43621 ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat ggccagaaaa
    43681 attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca gacgaagcga
    43741 tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc gcggagcggt
    43801 tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg ccgccgccgt
    43861 tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat taccgctacc
    43921 atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg gtcgattatt
    43981 cgcgtcacgc cgtccttctc ggcagtagtg gaatggcgga gctacgcttt attcgtaccc
    44041 acggcagccg ttttactccc caggattgca cactgtttaa ctggctggct cgcattatca
    44101 ccccggttct gcaatcatgg ctcaatgatg aagaacagca ggtggcgctg cgtttgctgg
    44161 agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg ctgtcacatc
    44221 ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt ttcggtctgg
    44281 cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc ctgtggtgca
    44341 gcgatctttc tgcctcacat tgtgcgtgtc tgccacgcaa tatgcctggc gacagtgtat
    44401 tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca gatgatctgt
    44461 ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc gaatctgcgc
    44521 tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg gcgcatacct
    44581 cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc gatcgcatcg
    44641 ctattgccgt tggcaatgcc gatgcctggc gtagaatgac cgatttgcag gaaagtttgc
    44701 agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc ggtgacatta
    44761 tctatcaaag ccaggcaatg gaagagctgc tccagcaggt agatattgtg gcgaagagcg
    44821 acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc gccagagcga
    44881 tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc actgccatcc
    44941 ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt actggtgcga
    45001 ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt ctcgatgaaa
    45061 ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag gaacgggaga
    45121 ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt gccgccacca
    45181 accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc ttttatcgcc
    45241 tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat atccctcttt
    45301 tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt gacgccatcc
    45361 cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg cgcgagctgg
    45421 aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat ttacatctta
    45481 atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt cgcagttcaa
    45541 tggcgcagtt gctgcacccg acgacgccag agaatgacga agaagaacgt cagcgcattg
    45601 ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg gcgacacgat
    45661 tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc tcggttcgca
    45721 ggtgttgtaa tctgcttttg caggagtatg catgagaaac aaactctctt tcgacttgca
    45781 gttgagcgcc agaaaagcgg caatcgctga acggattgcc gcccataaaa ttgcccgcag
    45841 taaagtgtcg gtctttttaa tggcgatgtc cgctggcgtg tttatggcga tcggatttac
    45901 tttttacctt tccgttatcg ccgatgcccc gtcttcacag gcattaaccc atctggtggg
    45961 cggcctttgc tttacacttg gctttatttt gctggcggtt tgcggcacca gcctgttcac
    46021 ctcgtcggta atgacggtaa tggcaaaaag tcggggcgtt attagttggc gaacttggct
    46081 gattaacgca cttctggtgg cctgcggtaa tctggcaggt attgcctgtt tcagtttgtt
    46141 aatctggttt tccgggctgg tgatgagtga aaacgcgatg tggggagtcg cgattttaca
    46201 ctgcgccgag ggcaaaatgc atcatacatt tactgaatct gtcagcctcg gcattatgtg
    46261 caatctgatg gtttgcctgg cgctgtggat gagttattgc ggacgttcgt tatgcgacaa
    46321 aatcgtcgcc atgattttgc ccatcaccct gtttgtcgcc agtggctttg agcactgtat
    46381 cgccaatttg tttgtgattc cgttcgccat tgccattcgc catttcgccc ctacctcctt
    46441 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtca gccattttat
    46501 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
    46561 tatttgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
    46621 gttcgtcggg ctgaagatta ttcttgcgcg atttgcccat cgggccaggc gtggatcacg
    46681 gctttgatca gcgtcgccaa tggaatggcg aagaatacgc cccagaatcc ccacaaacca
    46741 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
    46801 aacaccggta ctagcaggtt gccgtccagc gcctgaataa tcagatacac cgcgaagcag
    46861 ctccagaatt cagtacctgc gccaaactgg aatagcgcca cgccaaccac cggaatggtc
    46921 accacaaatg cgccaatgta cggaataaga accgagaagc cgaccagcac tgccagcagc
    46981 agcgaatagt tcagcccgaa gagcaagaac cccagccagg tggcgatccc caccacgatc
    47041 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
    47101 tgtcctgcca gcccacggtt acgcggcagc acccggcgaa cggcgttcag catctgctct
    47161 ttgtctttca gcaggaagaa gaccatcaat ggcaccagca ccagatagac ggctatggtc
    47221 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
    47281 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa cgccggatag
    47341 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
    47401 attaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
    47461 ataccgacaa aaaccaccaa tacaa
//
LOCUS       DADLSE010000014        47281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLSE010000014 DADLSE010000000
VERSION     DADLSE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.014"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..523
                     /gene="comR"
                     /locus_tag="JN692_000813"
     CDS             <1..523
                     /gene="comR"
                     /locus_tag="JN692_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7263197.1"
                     /translation="EATSLADLVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAK
                     HEAQLFCEEKSVESALADYFAAIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKS
                     RHAMQERTLQQFLCQRQARGEIPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQI
                     AGTTLRLWPELVK"
     gene            complement(585..1124)
                     /locus_tag="JN692_000814"
     CDS             complement(585..1124)
                     /locus_tag="JN692_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7263198.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1351..2655)
                     /locus_tag="JN692_000815"
     CDS             complement(1351..2655)
                     /locus_tag="JN692_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAY7263199.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3063..3605)
                     /locus_tag="JN692_000816"
     CDS             complement(3063..3605)
                     /locus_tag="JN692_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7263200.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN692_000817"
     CDS             complement(3628..4653)
                     /gene="nagZ"
                     /locus_tag="JN692_000817"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7263201.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDAGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN692_000818"
     CDS             complement(4664..5488)
                     /gene="thiK"
                     /locus_tag="JN692_000818"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7263202.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN692_000819"
     CDS             complement(5469..6110)
                     /gene="lpoB"
                     /locus_tag="JN692_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7263203.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQLGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSVQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6125..6502)
                     /locus_tag="JN692_000820"
     CDS             complement(6125..6502)
                     /locus_tag="JN692_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7263204.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN692_000821"
     CDS             complement(6505..6864)
                     /gene="hinT"
                     /locus_tag="JN692_000821"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7263205.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            6995..7177
                     /locus_tag="JN692_000822"
     CDS             6995..7177
                     /locus_tag="JN692_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263206.1"
                     /translation="MLPYISPLFPFLPANALKRHSLFGLHDKYECVYFSFAFAFAFAS
                     KIIRTLNITAHHPPPA"
     gene            7210..9399
                     /gene="fhuE"
                     /locus_tag="JN692_000823"
     CDS             7210..9399
                     /gene="fhuE"
                     /locus_tag="JN692_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HAY7263207.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSSIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDHVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9459..10892)
                     /gene="ptsG"
                     /locus_tag="JN692_000824"
     CDS             complement(9459..10892)
                     /gene="ptsG"
                     /locus_tag="JN692_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7263208.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11187..11984)
                     /locus_tag="JN692_000825"
     CDS             complement(11187..11984)
                     /locus_tag="JN692_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7263209.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(11995..12999)
                     /gene="holB"
                     /locus_tag="JN692_000826"
     CDS             complement(11995..12999)
                     /gene="holB"
                     /locus_tag="JN692_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7263210.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(12996..13637)
                     /locus_tag="JN692_000827"
     CDS             complement(12996..13637)
                     /locus_tag="JN692_000827"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7263211.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13627..14649)
                     /gene="yceG"
                     /locus_tag="JN692_000828"
     CDS             complement(13627..14649)
                     /gene="yceG"
                     /locus_tag="JN692_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7263212.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14652..15461)
                     /gene="pabC"
                     /locus_tag="JN692_000829"
     CDS             complement(14652..15461)
                     /gene="pabC"
                     /locus_tag="JN692_000829"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7263213.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISSDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(15581..16822)
                     /gene="fabF"
                     /locus_tag="JN692_000830"
     CDS             complement(15581..16822)
                     /gene="fabF"
                     /locus_tag="JN692_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7263214.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(16909..17145)
                     /gene="acpP"
                     /locus_tag="JN692_000831"
     CDS             complement(16909..17145)
                     /gene="acpP"
                     /locus_tag="JN692_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7263215.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17356..18090)
                     /gene="fabG"
                     /locus_tag="JN692_000832"
     CDS             complement(17356..18090)
                     /gene="fabG"
                     /locus_tag="JN692_000832"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7263216.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18103..19032)
                     /gene="fabD"
                     /locus_tag="JN692_000833"
     CDS             complement(18103..19032)
                     /gene="fabD"
                     /locus_tag="JN692_000833"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7263217.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALDYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19048..20001)
                     /locus_tag="JN692_000834"
     CDS             complement(19048..20001)
                     /locus_tag="JN692_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7263218.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20069..21139)
                     /gene="plsX"
                     /locus_tag="JN692_000835"
     CDS             complement(20069..21139)
                     /gene="plsX"
                     /locus_tag="JN692_000835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7263219.1"
                     /translation="MKRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21220..21393)
                     /gene="rpmF"
                     /locus_tag="JN692_000836"
     CDS             complement(21220..21393)
                     /gene="rpmF"
                     /locus_tag="JN692_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7263220.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21445..21966)
                     /gene="yceD"
                     /locus_tag="JN692_000837"
     CDS             complement(21445..21966)
                     /gene="yceD"
                     /locus_tag="JN692_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7263221.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22165..22749
                     /gene="yceF"
                     /locus_tag="JN692_000838"
     CDS             22165..22749
                     /gene="yceF"
                     /locus_tag="JN692_000838"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7263222.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22861..23820)
                     /gene="rluC"
                     /locus_tag="JN692_000839"
     CDS             complement(22861..23820)
                     /gene="rluC"
                     /locus_tag="JN692_000839"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7263223.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24393..27578
                     /gene="rne"
                     /locus_tag="JN692_000840"
     CDS             24393..27578
                     /gene="rne"
                     /locus_tag="JN692_000840"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7263224.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27775..28728)
                     /gene="flgL"
                     /locus_tag="JN692_000841"
                     /pseudo
     CDS             complement(27775..28728)
                     /gene="flgL"
                     /locus_tag="JN692_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
     gene            complement(28740..30382)
                     /gene="flgK"
                     /locus_tag="JN692_000842"
                     /pseudo
     CDS             complement(28740..30382)
                     /gene="flgK"
                     /locus_tag="JN692_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(30448..31389)
                     /gene="flgJ"
                     /locus_tag="JN692_000843"
     CDS             complement(30448..31389)
                     /gene="flgJ"
                     /locus_tag="JN692_000843"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7263225.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKSSEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31389..32486)
                     /locus_tag="JN692_000844"
     CDS             complement(31389..32486)
                     /locus_tag="JN692_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7263226.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32498..33196)
                     /gene="flgH"
                     /locus_tag="JN692_000845"
     CDS             complement(32498..33196)
                     /gene="flgH"
                     /locus_tag="JN692_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7263227.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANVSRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33249..34031)
                     /gene="flgG"
                     /locus_tag="JN692_000846"
     CDS             complement(33249..34031)
                     /gene="flgG"
                     /locus_tag="JN692_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7263228.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKVQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34169..34920)
                     /locus_tag="JN692_000847"
                     /pseudo
     CDS             complement(34169..34920)
                     /locus_tag="JN692_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(34940..36868)
                     /gene="flgD"
                     /locus_tag="JN692_000848"
                     /pseudo
     CDS             complement(34940..36868)
                     /gene="flgD"
                     /locus_tag="JN692_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(36880..37284)
                     /gene="flgC"
                     /locus_tag="JN692_000849"
     CDS             complement(36880..37284)
                     /gene="flgC"
                     /locus_tag="JN692_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HAY7263229.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSAPRSYQANVEVLNTVKSMVLKTLTLGQ"
     gene            complement(37288..37704)
                     /gene="flgB"
                     /locus_tag="JN692_000850"
     CDS             complement(37288..37704)
                     /gene="flgB"
                     /locus_tag="JN692_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HAY7263230.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37859..38518
                     /gene="flgA"
                     /locus_tag="JN692_000851"
     CDS             37859..38518
                     /gene="flgA"
                     /locus_tag="JN692_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7263231.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38594..38887
                     /gene="flgM"
                     /locus_tag="JN692_000852"
     CDS             38594..38887
                     /gene="flgM"
                     /locus_tag="JN692_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HAY7263232.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            38892..39308
                     /gene="flgN"
                     /locus_tag="JN692_000853"
     CDS             38892..39308
                     /gene="flgN"
                     /locus_tag="JN692_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7263233.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMRQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(39348..40883)
                     /gene="murJ"
                     /locus_tag="JN692_000854"
     CDS             complement(39348..40883)
                     /gene="murJ"
                     /locus_tag="JN692_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7263234.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(40993..41916)
                     /locus_tag="JN692_000855"
     CDS             complement(40993..41916)
                     /locus_tag="JN692_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7263235.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(41918..42565)
                     /locus_tag="JN692_000856"
     CDS             complement(41918..42565)
                     /locus_tag="JN692_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7263236.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNVVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42576..43160)
                     /gene="rimJ"
                     /locus_tag="JN692_000857"
     CDS             complement(42576..43160)
                     /gene="rimJ"
                     /locus_tag="JN692_000857"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7263237.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43396..44604
                     /gene="mdtH"
                     /locus_tag="JN692_000858"
     CDS             43396..44604
                     /gene="mdtH"
                     /locus_tag="JN692_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7263238.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44668..45315
                     /gene="grxB"
                     /locus_tag="JN692_000859"
     CDS             44668..45315
                     /gene="grxB"
                     /locus_tag="JN692_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7263239.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45449..46009
                     /locus_tag="JN692_000860"
     CDS             45449..46009
                     /locus_tag="JN692_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7263240.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46115..47161
                     /gene="pyrC"
                     /locus_tag="JN692_000861"
     CDS             46115..47161
                     /gene="pyrC"
                     /locus_tag="JN692_000861"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7263241.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        11416 a        12792 c        11758 g        11315 t
ORIGIN      
        1 tgaagcgact tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata
       61 cgcggaattt accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg
      121 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
      181 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg
      241 cttcatgatc aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt
      301 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
      361 gcgcggggaa atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat
      421 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
      481 tgccggaacg actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc
      541 tcagcctgag cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc
      601 aaaatcagtt gcccgttact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg
      661 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
      721 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
      781 gagccctgga tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca
      841 tctttaccgc gaccaccacc aaactgatgc cctatcacgc cacctgcaac agcgccgagc
      901 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg
      961 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
     1021 gcgtattgcg ggccccgttc aaacacgttc agactggcca ctgccgctac gcccaacgca
     1081 gctgcgacac caatcccgat acccgccaac attgatttat tcacgggaac acctccttct
     1141 ttgcagttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
     1201 agcgcgggaa tgactggaaa tacttaagga ttcggaggag tctgaaggaa tgcctgatgc
     1261 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
     1321 gtttatgcca catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac
     1381 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
     1441 ctgatgcatt cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat
     1501 tgagccgcgc gtcaggttac ccatcaggct accaacggtg gagaagttcg acagcgatac
     1561 cagcgaacca tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
     1621 aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa
     1681 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg
     1741 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc
     1801 gatatctttc aggaagtctg gcgctttgat cccggctgcc cataccatca gatcagcctc
     1861 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
     1921 cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg
     1981 cggtaatgca ggcaaaatac gttctcccgc ttctaccagc gttacgttca gggcttcgtt
     2041 ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgacc gcgttgtgca attcagcgga
     2101 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg ttcactttgc catttgcgcc
     2161 caggttggca gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
     2221 gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga aatcgttaga
     2281 ggtgctaccc agcgccatga ccagggtgtc ataggcgatt ttacgttccg gaaccagcag
     2341 ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
     2401 aatgacggaa cccaactgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
     2461 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
     2521 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttacgtc ccagcttatg
     2581 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caatcttttt
     2641 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
     2701 taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa
     2761 taacatgaat ggtgcattgg tcataccaaa attgatgtta gtcaagtttt gttgcttaaa
     2821 agataggcaa ctttaccggg tgagatcaaa aaatagagtt tattgtttat tatttgagca
     2881 aattcggaag agtaatatag ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag
     2941 atgcgcagca tcgcatcagg cattgatgct cgactgtcgg atgcggcgtg gacgccttat
     3001 ccgacttaca gaattcggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc
     3061 atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat attcttgaat
     3121 ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcaactc ttcagatgtc
     3181 cgctggctgt taagtgcttc atcattacgc gacaaaataa ccagacaacg atcgcgattc
     3241 ttctcgcgga agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca
     3301 atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata
     3361 tcgcaaagaa aaccaatccg ttccgcccag tatccgccca ggccaacgcc gcaaattagc
     3421 ggacgctcgt caacgttcag ttgcaacatt ttgtccactt ctttaagcag atgctgcata
     3481 tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc
     3541 aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata
     3601 atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc
     3661 atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca tcagttcctg
     3721 tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag
     3781 attatctaac acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc
     3841 cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat
     3901 cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca aaacggtttt
     3961 cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc
     4021 aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt
     4081 agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt
     4141 tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc
     4201 atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc
     4261 gccaatcgcc gcgctgatat gcccggcatc cagcactggc gcaaagctga tatcaatatc
     4321 catagcgatc atttcactgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc
     4381 ttccattccc aacagcgcag caaaggattg tgctgccggt aagcgggtaa aaccttcgcg
     4441 aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgatgc
     4501 tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga tcatgatagt tacgcgtaaa
     4561 gagaatcagc cctcccacca gcggatgcgc cagtatttca cgctcttccg cgtccagttc
     4621 gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt tttattaata
     4681 gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc
     4741 ggtactcaaa ccaccctcct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac
     4801 gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt
     4861 gctgttcagt attttccacc cacaccgccg ccagttccag cgcgatatca ccatctccgg
     4921 catactccca gtcgatgagt tttaaccctg atgcgctatg cactaaattt ccggcgtgga
     4981 catccatatg caatggactt aagcgtaaag gtcgtggttc ccgcgctttg cgcagacgtt
     5041 ttaacattcg cagccaaccc acagtccgcc gcgccggatc gctttgctgc cagtacagtt
     5101 ccagtaaggg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt agataataca
     5161 gcaagcctgc cagttcgttg gtatccggca aatacgtttt taccgcgccg ggcagatagt
     5221 cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt
     5281 gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct
     5341 gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actaccgcca ctaagcccac
     5401 tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgcg
     5461 tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc
     5521 accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac
     5581 agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg ggtgcctaaa
     5641 ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagttgctg gacggaaacc
     5701 agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca
     5761 ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc
     5821 accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa
     5881 tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt gctcaattgg gcctagctgc
     5941 tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt
     6001 tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg
     6061 agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac
     6121 ccctttacaa atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt
     6181 acagcgttac cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca
     6241 tctccagccc tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc
     6301 tttcgttata aagcgttgag gatgctgaag gttgaatatc agacgtcgaa aggacgggct
     6361 tttctgcact gatgcccgca gccaataaag atgactccat caccagcgat tgctcatcat
     6421 tcaccggaat ttccggatgt gaacgacagc ccaccagcag taacaacacc agagacacca
     6481 gcccaaagca tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg
     6541 acggccaccc aacaagtgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt
     6601 attcatgatc agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc
     6661 tacggtgatc atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg
     6721 aatgaggata ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa
     6781 cgccgttacc agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt
     6841 gctgaatata gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc
     6901 atgctacgcc gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa
     6961 ccttaactca cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt
     7021 cccatttctg cctgcgaatg cgctgaaaag acattctctg tttggtttac acgacaaata
     7081 tgaatgcgta tatttctcat ttgcatttgc atttgcattt gcaagcaaaa ttattcgcac
     7141 actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccaag
     7201 agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagc catcaccaaa
     7261 ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca
     7321 ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga tgatggcgaa
     7381 aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac tcaacgtgat
     7441 attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca gttacaaacg
     7501 ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt
     7561 gctctttatt attcccgcgg attccagatc gataactata tggttgatgg tatccccacc
     7621 tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact gtttgaacgc
     7681 gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc atctgcggca
     7741 attaatatgg ttcgaaaaca cgcgaccagt cgtgaattta aaggcgatgt ctcggcggaa
     7801 tacggtagct ggaacaaaga acggtatgtg gctgatttac aaagcccact caccgaagac
     7861 ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg gctggaccgc
     7921 tacaatagtg aaaagacctt cttctcgagc attgtcgatg ctgatttagg cgatcttacg
     7981 acgctttcag ccggttacga atatcagcgc attgatgtta atagcccaac ctggggcggt
     8041 ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgcgcacg cagtaccgca
     8101 cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct gaagcagcgg
     8161 tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga atttgacagc
     8221 aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt tgggccatac
     8281 agtaattatg gacctggctt tgatcatgtc ggcggcaccg gttggaacag tggcaaacgt
     8341 aaagttgatg cgctggattt gttcgctgac ggtagttatg aattgtttgg tcgtcagcac
     8401 aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttacttcag ttcatgggcc
     8461 aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaaacc
     8521 gactggtcac cacagagcct ggcgcaggac gataccacac atatgaaatc gttatatgct
     8581 gccactcgtg tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac
     8641 tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt
     8701 ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc tattttccag
     8761 ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac
     8821 gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc
     8881 cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc
     8941 gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac
     9001 ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatat tgcagaggat
     9061 aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc
     9121 tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc
     9181 gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg
     9241 ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc
     9301 aataacctgt tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca
     9361 ccgcgtaatt tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca
     9421 gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca
     9481 tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tcgcctgaac accagaacca
     9541 gcaaccacta cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca
     9601 tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta
     9661 ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct
     9721 tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag
     9781 atggtgtagt aaacaatcgc ataaccgata ccgacgatcg ggaacagcca cagtttgctg
     9841 ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt accgtcacgc
     9901 atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac
     9961 aggatcggcg caacgaacat gaagaagaac tcgatcggct cggtgatacc ggtcaggaac
    10021 gaggtcagcg ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca
    10081 gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaaaccacca
    10141 gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc
    10201 tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg
    10261 tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc
    10321 gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa
    10381 atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag
    10441 cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac
    10501 atgtacgctg cgatcgcacc ggagataatc cctccgagta cgccagtatc cgccaagtgt
    10561 ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt
    10621 ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta
    10681 aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct
    10741 gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg
    10801 cccagcagaa tacctgcgat aggtagtacg gataccggca gcatcagcga tttaccgacc
    10861 ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga gtatgggtgc
    10921 tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa
    10981 gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt gacggctatc
    11041 acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata
    11101 tttcaaacgc agttattcac cgcccaattt tcggcggtga actttactcg ttttagccat
    11161 taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg
    11221 tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct
    11281 ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct
    11341 cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg atccaggggg
    11401 acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat gccggaaaag
    11461 gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag
    11521 tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggca
    11581 cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga
    11641 tggatgaagg actcttgctg acgtacttta gtttccggtg tgtaataata atccagcccg
    11701 gtttcaccca gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg
    11761 tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg
    11821 cctaccagat cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat
    11881 ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca
    11941 taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag
    12001 atgaggaacc ggtagcacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc
    12061 ggtgatgaga agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca
    12121 aacatccccc agtatagcct gcaggcgcga gggagaaaga tggtttgcca gttcggcgac
    12181 caggcccggc acatcaacat tggtcacctg cgcagcgcca tgatggcgtt ttagcgcatc
    12241 catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat taagggccgc
    12301 cagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac acaatgtttc
    12361 acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgctaggcg aaccggcgct
    12421 taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaagt
    12481 cacggcgtac tgttctggag gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt
    12541 tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caagtttctg ctggtggctc
    12601 ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac
    12661 ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg
    12721 taccgcatca atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg
    12781 atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg
    12841 cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc
    12901 cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag
    12961 tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag
    13021 tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg
    13081 gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag
    13141 aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag
    13201 ccaacttccg gggtaacatc gagatagagc gttaagtcgg ggcgaaaatc cccgagaaca
    13261 gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc
    13321 tgagtggaga gatcgtggcg atcgccaatc acccaggtgc cgttagccag cgcaggtttg
    13381 atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg
    13441 gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca
    13501 agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc
    13561 gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg
    13621 atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt
    13681 atgactggca agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag
    13741 atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat
    13801 cgcacccggc ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc
    13861 tgcacgagaa agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt
    13921 ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc
    13981 actggcaacg gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc
    14041 tttataaggc agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt
    14101 cttgtgagct cgcttgagta acgcgacatc ggtggtattg gcggtataca tcaaggtgtc
    14161 tggccagaac caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt
    14221 ggcgtactta tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag
    14281 gtagtcgctc agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct
    14341 ttccaacaat ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc
    14401 tttaaagtga gaaagatccg gttcgatacg cagcagccat tgaaaaaccc gtgggcgatt
    14461 gatgatctta tcagcataaa gctgttcacc gagcgccaga cgtccggtcc ctggcttcag
    14521 ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca
    14581 gacgcccaca ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac
    14641 ttttttcatg actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct
    14701 gacgaaaagg tgacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa
    14761 ataaccatct catctgcctg caacgcctcc tccagcgagg cttgcacttc gacaagctga
    14821 taagaggatt gtgccagtaa acggatacag aattgtcgca taatgccgtt aacacctgcc
    14881 tgatccagtc gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag
    14941 cattccgtaa cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca
    15001 agatgagagc gaatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga
    15061 ggattgcgcc ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg
    15121 tcgtaatgtg caggataagc cgtaacggag agaatccgcg ttgctggtcc gctgttcaat
    15181 gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc
    15241 tgttctgctg ccagcgtttt catctcttgt tcaagctgag gccagaagtc actggaaatc
    15301 atcaaccgct gacaagcatc ctgtagtcgc tggatatgcg ccgataacaa actgacttta
    15361 ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt
    15421 actgccagcg attcctgctt ataaccgtta attaagaaca taccggctcc ttatcagaga
    15481 attgttagtg tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag
    15541 gcccgcaagc ggacctttta tatgggtgga aaatgacaac ttagatcttt ttaaagatca
    15601 aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga
    15661 cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt
    15721 tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg
    15781 cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc
    15841 ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg
    15901 tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca
    15961 gagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat
    16021 aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt
    16081 tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat
    16141 cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg
    16201 cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg
    16261 caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca
    16321 cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag
    16381 tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac
    16441 gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc
    16501 cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat
    16561 cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct
    16621 gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg
    16681 ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca
    16741 gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca
    16801 caactacacg acgcttagac acgtttgtcc tccagggagg gaaaaatgat tctagtggga
    16861 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
    16921 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
    16981 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
    17041 cgcgcctagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
    17101 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
    17161 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
    17221 aaatcccggc aggtcttacc acgattttac gttattttga ggccttcccc cccaataacg
    17281 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
    17341 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
    17401 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
    17461 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
    17521 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
    17581 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
    17641 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    17701 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    17761 gttagtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    17821 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    17881 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttggc
    17941 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    18001 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    18061 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    18121 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    18181 caggccagta agcaccttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    18241 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    18301 acggatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa cagaaactgt
    18361 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    18421 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgccagcagc
    18481 tttacaggca gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc
    18541 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    18601 cgcaatagac gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttccggtac
    18661 ggcttcttgc atgaacttac cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    18721 cacgccagcg caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    18781 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    18841 tggctgggtt tgccaggttt tattcagttc ttcagctggc ccctgctggg tcagcgccca
    18901 caggtcgtag tccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    18961 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    19021 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcggagc cccaagtgaa
    19081 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    19141 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt
    19201 caccacgaca ttgtccatcg acataccgag ttttttcgcc gttgcactga taatacgcag
    19261 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    19321 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    19381 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    19441 ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg attcccggct cttcagaggc
    19501 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    19561 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    19621 tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac
    19681 gtcaaatgcc gggcaacctt taatgcccaa catgctttga atctgacaag ctgcgctcgg
    19741 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    19801 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    19861 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    19921 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    19981 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    20041 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    20101 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    20161 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    20221 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    20281 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    20341 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    20401 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    20461 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    20521 ggattgtttt aagcaccgct gaggcatccc gaatactgtc gagacccttt acttcttctt
    20581 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    20641 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    20701 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    20761 gctcaatccc ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat
    20821 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    20881 ttgaactccc acgactggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    20941 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    21001 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    21061 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    21121 ccagggttag acgtttcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    21181 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    21241 gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt
    21301 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    21361 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    21421 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    21481 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    21541 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    21601 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    21661 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    21721 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    21781 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    21841 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    21901 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    21961 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    22021 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    22081 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    22141 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    22201 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc agaggtcgac
    22261 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    22321 gcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    22381 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    22441 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    22501 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctaagcgag
    22561 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    22621 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg
    22681 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    22741 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    22801 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    22861 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    22921 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    22981 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    23041 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    23101 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    23161 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    23221 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg
    23281 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    23341 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    23401 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    23461 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    23521 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    23581 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    23641 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcacctt tacgcaaaat
    23701 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg
    23761 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    23821 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttatattt
    23881 agttttgcac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    23941 ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct
    24001 ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg tccatccttg ttaaaacaag
    24061 aaattttacg gaataaccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac
    24121 gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa
    24181 tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga
    24241 aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac
    24301 gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta
    24361 gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc
    24421 agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctggatatcg
    24481 aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg
    24541 aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac
    24601 taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca
    24661 ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg
    24721 gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga
    24781 tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg
    24841 aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca
    24901 ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac
    24961 actgggaagc catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg attcatcagg
    25021 agagcaacgt aatcgttcgc gccttccgcg attatttacg tcaggatatc ggcgaaatcc
    25081 ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc
    25141 cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc
    25201 agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca tccggtggtt
    25261 ccattgttat cgacagcacc gaagcgttaa cagccatcga catcaactcc gcacgcgcga
    25321 cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc gccgatgaga
    25381 ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca
    25441 tgacgccagt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg
    25501 accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgcc
    25561 agcgcctgag cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta
    25621 ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg atcgaagaag
    25681 aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtgcca atcgcttctt
    25741 atctgctgaa tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag gacggtgttc
    25801 gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc
    25861 gcaaagggga agaaacgccg accttaagct acatgctgcc gaagctgcat gaagaagcga
    25921 tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg
    25981 caacctttgc catgccggat gtgccgccag cgccaacccc agctgaacct gccgcgactg
    26041 tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc agctcctgca caacctgggc
    26101 tgttaagccg cttcttcggc gcactgaaag cgctgttcag cggtggtgaa gaagccaaac
    26161 cgaccgagca accaacaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca
    26221 aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta
    26281 ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaaa
    26341 ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg
    26401 acgagcagca agcgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg
    26461 cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg
    26521 aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc
    26581 gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg
    26641 ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc
    26701 tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg
    26761 acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc
    26821 agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg
    26881 tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac
    26941 gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga
    27001 tggtgactga ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg ccagttgttg
    27061 aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg
    27121 ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta
    27181 ccgagtctga tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg gtggttgaac
    27241 cgcaggaaga gacggcagat attgaagaag ttgccgaaac tgctgaggtt gtggttgctg
    27301 aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca gcagaagttg
    27361 aaacggtagc cgcggttgaa ccagagatca ccgttgagca taaccacgct accgcgccaa
    27421 tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt gactggcagc
    27481 gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca acgcatcatg
    27541 cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa agtaatcaag
    27601 ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta aaattcatca
    27661 aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg ccccggcgat
    27721 cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc gagcttattt
    27781 gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg atgcctgcaa
    27841 tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat caaccagatc
    27901 gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca gcgactccag
    27961 tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca gcgagttttt
    28021 cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg cttcgctatc
    28081 cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa gattggtttc
    28141 agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga aaattttgtc
    28201 gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa tactttctgt
    28261 tccgccgaca taatcaccgt tagcctcact aaacggcgca gtctctgttt tataaccggc
    28321 aaaaatgtag cgcccattac cgtcagtggt gttcgccaga ttcagcaact ggtcacgaag
    28381 cccctgaata tccgtagcca gcgaggcccg gtcatcgtca ctcaaggtgc cattgctggc
    28441 gtagacaatt ttttcctgag cattctggat agcagtggtg acctggctaa gtacactctc
    28501 ttccagtgac actttttgag tggcgaaagt acgtgccagc gtgtactggc tgttttgcgc
    28561 ctgtgcctgg gagagaacaa cagcttgtga tgcagcaatg ggatcgtctg aagggttaac
    28621 gactcgctta cccgtcgata tctactcgcc gtacttcatc cattctgcct gagaattggt
    28681 gatgccgcgc atgttttgct ggtacatcat ctgggtactg aaacgcattt tatctcccct
    28741 tagcgaatgt taatcagagc atcaaaaatc gtgtttgccg tctgcagaac ctgcgcattc
    28801 gccaggtaat actgctgaaa acgttgcaga tttccgtact cctcatcgag attgacaccg
    28861 gaaatcgact gctgctgatt ggaaagctgc gtcaccacat taccttgcgt ggtgctactg
    28921 gttttcaaca tcgcggtttt attaccgata tcactcacta acgaggcata agcgtcgtta
    28981 aaggatttcg caccgcccac cgttttactg ttgctttgca gatccagcag ggcctgaccg
    29041 ttgcggttgt cgctatcacc cgcatcttct tcgctcgcca tcgcgatttt cgcttcgtcg
    29101 gtgattaata catccatgtt gacgatggcg tcacttactg gtttcagcgt gaagctgtcg
    29161 ttaacggcag gcgttcctgt aaacgtcaac tccagaccat caaatgccac tttaccgttg
    29221 gcatctggtg tcgccgtaaa agtggtattg ctggcaaggc gggtgacctg ccactgatta
    29281 ttatcgaacg agattttgta atctgtcgcc agtaccacgg aggcatcagt taccgtggca
    29341 ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa ccgcgggctt accgatagta
    29401 aagaaatctt caccagcatc gccgttagca tcaaatccgg ctttgtgttg gctgttgaaa
    29461 gcctcggcaa atgccagcgc cagttgtcca agcgtattac gcgtctggtc cagatcctga
    29521 gaacggaatg tcagaatgcc gcccagcgac ccggtattca gtaatttctc cgggatctca
    29581 atattgcctg ccgtcccatc aacgtaagcg acagtcgtac gagaagggtc ggcgctggaa
    29641 ggaactgccg ccagttgccg cgccgtactt ccctgaacca gtgagtaacc attggcatcg
    29701 tgatgttata agtaccgcca tcctgaacgc tgacttctac gccaacaatc tggtttaatt
    29761 cgctcaccag ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc
    29821 ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct
    29881 gatcaacgct ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat
    29941 cggtggtttt aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgccg
    30001 ccgggtcttc cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg
    30061 ttgccagcga agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc
    30121 gggcggtcag gccgctactt tgcgtctgcg cagcacgtaa ctggttggta ataaacgtat
    30181 catactcacg ctgcacacca gaaacgtaga cgccattgcc aacccagccg ccagcgccca
    30241 acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc
    30301 tggagatatt attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg
    30361 cgttattgat caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc
    30421 ggcagcgacc cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt
    30481 tttgctcacc ttgtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc
    30541 atagtgagga tcggtagcat agcccgcgtc ctgtaggacc tgcgccccct gttccgcact
    30601 cgcggcggtc gtcacggcgg cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga
    30661 caatgcttcc agatacgagc tgtagacccg aaacttcgct tttactttct tcgcttcgcc
    30721 gttttcatat tcagtcgtgg tgatttcagt gacttgccct ttccagttgc cagaggcttt
    30781 gacaccaaac aggttatagc ttggctcgcc gttttcgcgg cggatttgcc gttgccccca
    30841 accagattca agcgccgcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct
    30901 tgccagttgg gcgggcagcg agagttgcgc gaggaatgct ctactgtcac ccggcagcga
    30961 atcatcgtag ttacgtggta cggccttttg caccagctgc gaaagcgtct ggttttgata
    31021 acgcaccacg gtttcgagcg ggaatttcat cggtgctgct ggcgtggact cctctggcaa
    31081 tggttgttct ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc
    31141 cgtcatctgt tgggcaattt gctggtcata catactggta tacagacgag tgtgctcgct
    31201 gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa
    31261 catcccttcc acctgacggg ccaccggacg gatatttgcc gccggatctt cgctcgattt
    31321 cgcctttagt tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc
    31381 gctgatcatc agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc
    31441 aaaatagaca tcagatccat cggcgtagcg cccagcgcat tgagcgcacg caccacgtta
    31501 ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taaatcgatc
    31561 tgcgtttgtg gagtaacaac agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca
    31621 ttggcctgac gattaactgt tactgagaga tttccctgcg ctaccgcgca gctgtcgagg
    31681 gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc
    31741 tgcggggtga cattaacctg catattctgg atatcggcaa ggaaacggac ctgggaactg
    31801 ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat
    31861 ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta
    31921 agttgcaaat taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc
    31981 gcaccattgg tgatccgtcc accgttcagt tggttaacct gaacactgct accgcccgcg
    32041 gaggctcctg cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca
    32101 acgcccttaa gcggtgtcat caacaacgta cctccacgca agcttttggc attacccatg
    32161 gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc
    32221 accattaccg cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc
    32281 agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca
    32341 ccggtgccat ccagccccac taccaggcca tagccaatca gtgagttttg ccttaccccc
    32401 tgaacactgg tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga
    32461 attaatgcag agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga
    32521 agaaacgctg caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt
    32581 caatacgcgc atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg
    32641 gattaaccac gcccgagaag cgaataaatt cggtaccctg attaatggca atctgttttt
    32701 cacccaccac atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc
    32761 cgctaaaggt attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg
    32821 cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa
    32881 agccaaaatt agttttaccg tcacggctga cattcgcaga ggagcttttg ctggcgctga
    32941 cgttctcctg caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa
    33001 acagcggttg atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga
    33061 cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg
    33121 gtatccaggc gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg
    33181 cagcgttttt ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg
    33241 gttatgcctt agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta
    33301 ctgttgattt cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg
    33361 acgttagacg tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg
    33421 ctttcgttcg gtgcaccgga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc
    33481 agtccggtat cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc
    33541 tggccttgtt gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc
    33601 gccggaatgg tgatcgctgg ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc
    33661 tgatccacct ggaaagagcc gtcacgggta taggctgatg agccatctgg caacattacc
    33721 tggaaaaagc cctgcacttt aatcgcgaca tctttgctgt tattggtctg cgacagattg
    33781 ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg
    33841 gagggtaagg tggtttgttc ggaagactgt gcccccggct ggcgaatggt ttgataaagc
    33901 agatcttcaa acaccgcgcg ctgacgctta aaaccgttag tactgacgtt tgccaggttg
    33961 ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc ccgttttggc gatccataat
    34021 gaactgatca tataatgtcc tgtagtggat tgctgacaag cagagcagaa gcacgcgaca
    34081 gtgtgaattt cccctcaccc taaccctctc cccaaagggg cgagggaata gtccgcgcgc
    34141 ttttattgca atgtcatcaa tcgttcaatt aactcatcga cagcagttgg ttggcacggc
    34201 ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg
    34261 caatcatgtc gctcattgcc gcaacggcat tgacgttact gccttccaga acccccgaca
    34321 tcacacgcaa ggttggatct gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc
    34381 ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcatcagtt
    34441 tcagacgccc tactggcgca actgtatttg ccggatcgcc cggattgagc gccgagattg
    34501 tgccatcggc agcaatcgtg atttccgccc cttccggcac agcaattggc cccgcctcgc
    34561 ctatcaccgg atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat
    34621 tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc cagccatcct gctgcaacgc
    34681 aacgtccagc gggcacgagg tgtaatccat cttgccgggc gtcatgtctg cacccggtgt
    34741 tgacgccgtt accagcgtgc gcgtgggcag tgaaagccct tccaccggca ctgcgcgcaa
    34801 ggcgtttaac tgcgcgcgaa aaccgggcgt agaggcattg gccagattac tggcggttac
    34861 cgcctgttga ttcagcgtct ggctggctgc tcccatcgca gtataaattg cgtgatccat
    34921 tgagctatcc cgtcagcgat tagcgtaagt taaccagcgt gttgaggatc tggtcctggg
    34981 ttttgatggt ctgggcgtta gactgatagt tacgctgggc aacgatcata ttgaccagtt
    35041 ctttactgag atcgacgttg gacgcttcca gcgcaccgtt ggtcagggtg ccaaagtttc
    35101 ccgtcccggc tgtccccaac agcgccacgc cagaagattg cgtcgcagac cagacgttgt
    35161 cgccttcgga tgccagacct tcgttgttgg caaagttcgc cagtacaatc cgccccagca
    35221 gttgggtttg ttcgttggaa tagttgccga caaccgtacc gtcatcattg atttgataac
    35281 tcaccagatc gcccggtttg tagccattct gggtggttgc cacaatgttg ttagcgccgg
    35341 tattttgctg catggagttg aggaagctca gactaaacgt ggcgggttct gcaccgttaa
    35401 ttgcgccggt tgcgatatta ttcgccatcg caccatccac taatgtgcca ttagcattaa
    35461 attccagtgt tgtcgctgtc ttcgcaatgc tgtttggatc actgctatcc tgggtgtaga
    35521 cctgccagtt attatccccg gtcttcacaa aatagacgct catgtcatga gcattaccct
    35581 gactgtcgaa aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga
    35641 atggcgtaac agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg
    35701 tggtagtttt cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt
    35761 gctgaatagt cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat
    35821 tcaccaggtt acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac
    35881 cgttgctgtc taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc
    35941 cggtgttggt ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca
    36001 gtcccacttt cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt
    36061 aggtggcgga gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta
    36121 atccgctaac cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta
    36181 tctgccgtac ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc
    36241 tgttgccgcg gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg
    36301 taccaccgtt actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat
    36361 cagtcaacgt accgtcccag gtgaaactgg gaactccggc ggtcagttca ccaatatcaa
    36421 tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct
    36481 gttgcagctc aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc
    36541 ccgcaagaac agttgtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta
    36601 acgactggct gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa
    36661 tcccgctgac cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat
    36721 tggtcgggtc ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat
    36781 ctgcggcgtt gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg
    36841 gatcggtggt ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt
    36901 tttcagcacc atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtgg
    36961 cgccgacatg gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc
    37021 ctttgcatct gccagcggat tacctggttc ataaaccagt ttgtccgggg cctgactttc
    37081 tataacatcg gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa
    37141 taccacctgt tttgcccgat atggctgtcc atcaggaccg gtcacgctat cagcatttgc
    37201 cagattactg gccgccacgt tcaggcgctg ggactgggca gttaacgccg acccggcgat
    37261 atcaaaaata ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc
    37321 tttgatttgc ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa
    37381 ctgggtgcgt tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat
    37441 acggtattgc agttctgcgg taggaggcgt cagcgcctgc gccgggatgt gttgcgttga
    37501 ggtcatcgtc agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag
    37561 ttcactggca aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt
    37621 tgctgccagc acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa
    37681 ggcggcgtcg agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct
    37741 taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc
    37801 cgttgatggt cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat
    37861 gctgacaata aaacgtagcg tggcgatcat cgcgatactg ttcagtccgt taagtacggc
    37921 gagcaatctc acatcgcaat tgcacaactt ttttagcgcc caactcgtgg gggtaagtga
    37981 tgaggttcgt gtttctattc gtacagcgcc caatctacta ccgccatgcg agcagccatt
    38041 gctttcgatg agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg
    38101 taacgacaaa cgatatttac aggttaatgt gcaggccaca ggaaattatg tggttgccgc
    38161 gatgcctatt gtgcggggag gaaagctgga agctggcaat gtcaaactga aacgtggacg
    38221 gctggatacc ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag
    38281 cctgcgcgat ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg
    38341 ggtaaaagcg ggacaacgcg tcaatgtgat cgccagcggt gatgggttta gcgccaacgc
    38401 agaaggtcag gcgctgaaca atgcagccgt cgcacagaat gcgcgggtgc gcatggtatc
    38461 gggacaggta gtcagcggcg ttgttgatgc agatgggaat attcttataa acctgtaagc
    38521 tgttaaagat tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa
    38581 tgaggaataa accatgagta ttgatcgcac ttcgcctctg aagcctgtaa gcaccgttca
    38641 accgcgcgaa accactgacg cgccggtaac gaacagccgg gcggcaaaaa caaccgcctc
    38701 caccagcacc agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag
    38761 tgatatcaat cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa
    38821 aatggacacc ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag
    38881 taactgatcg tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg
    38941 atctcaaaac ggtaatggat caagagcagc aacatctctc tatgaggcag atcaacggca
    39001 gccagttgca atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg
    39061 aacagttacg caagaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc
    39121 aggaaattac tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac
    39181 tggaaggaca gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac
    39241 cgacgctata tggggcgaac ggtcagacct caacacccca tcgcggcagt aaaaagattt
    39301 cgatctgaat attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg
    39361 cgggcaaatt ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc
    39421 cccgccagca cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac
    39481 tccggcatga tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc
    39541 agacgcaaca gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac
    39601 tgccagtaaa gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg
    39661 gcatgtttca acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg
    39721 atggcaattt tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact
    39781 ttcactacaa tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc
    39841 agcgcatcaa acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacctgaa
    39901 agaatgccca acgcaaccgc actcggcagt gccaacagga aacaaagacg caacccccag
    39961 tccatcaaac ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc
    40021 agcaaaatgg tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg
    40081 gcgtaataca tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg
    40141 attaaggaga tctggctcac agagacgcca aggatcgccg gccccatctg tttcaccacg
    40201 cgcattgctc cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc
    40261 aggtgcggta gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc
    40321 gccagcaccg gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg
    40381 ttaagcagtg ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct
    40441 cccaccagcg acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg
    40501 ctggtcaggg caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc
    40561 cacggtgcgg cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca
    40621 gaaacataag agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc
    40681 agaatcggta caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta
    40741 ggaagtttaa aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg
    40801 acaattgcgt ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg
    40861 gccagcgatt ttaataaatt catcggtgtt ctaatccgga cccacgggca aaacgcctgc
    40921 aaggcaggcg ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag
    40981 atgttacagg gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc
    41041 cagcactgcc tgttcgccgg cggtttgcgg aactgtctga ttttgcacgc attcaatgaa
    41101 gtgccgcgca cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg
    41161 tttattcact acaccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat
    41221 gagcgcaccg tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat
    41281 acaagtggtg atctgcaaag gaccggccga aaaatggtgc tcggcaaaca gcatttcgcc
    41341 agcgtcgtta gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag
    41401 cgcggtatcc accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc
    41461 gacgctattg ctacgatgtt tatccattct tagcgaggct gcggtggcga gttgcgtttt
    41521 taactcaccg tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt
    41581 ccgcgccgcc agttccacca gcctttcagc atcgcgcaga ttttctgcca gcggtttatc
    41641 gacacagaca tgtacccccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt
    41701 gctggagtgc acaaaaaccg catcacaact ggcggcaagg ctggataacg aatcggcata
    41761 aggaatgcgc cagctgtcac aaattggcag ggctttggcg cgcgtaggcg accaggctcc
    41821 ttgtaacgtc cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc
    41881 gcctaatccc actacgccga tacgtaattt tttcacttta atctcccaga tgggccagca
    41941 acgaatcaag acgttgtttc agttctgcca cttcgatttc cagggcttcg acgcgggtct
    42001 gtaaatcacc gtcaaccacg ttcgacatag ccgttaccgc cggctgatct tcaacctcac
    42061 cactgaaaag atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca
    42121 caaaaggacc atcttcgcga ttcgccagtt gttccagcgt cgactccact tccgccatat
    42181 cgctgaattc atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac
    42241 gcaataacaa cgtggtgatt aacgccactt ctgcggcgct cagtttcaga tcgccaaatt
    42301 ctgaattaca aaaacgttgc tcatatttgg tgacccgatt accaaaaccg ctcactgtgc
    42361 gtagataatg acgtttgacc agattatcca gctgttcctg cacttcggat tcactcagat
    42421 tcatgaccgg ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga
    42481 gcgggtattg ctccggcgtg gtcacctgct tttccagcaa gcaaccaatt acgcgagctt
    42541 ccagggcagt aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg
    42601 gtagttaatg ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc
    42661 gcatagcctt ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc
    42721 ggcatataat tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct
    42781 gcggtcaggg cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg
    42841 agatagcagg catgaaaaga gccgcgaaca acattggaaa aattggcaac gccaataatc
    42901 tctttttcgt ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg
    42961 ttaatcatcc ctagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact
    43021 ggctcccagg gcttgaggaa atggcgattc tctgcgtaat aatccgccag acgccaggca
    43081 tcacgatcat gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg
    43141 ttactgcgat agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt
    43201 aagtattact atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca
    43261 ttattcacat atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt
    43321 ccccgaagtg atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat
    43381 ttcccgggag gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg
    43441 ctcatcgata atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc
    43501 cgcttcgttg atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc
    43561 cagtttattc agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc
    43621 aaaccgatga ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc
    43681 gcccacgaac cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg
    43741 tttgatccgc cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgtggtcgt
    43801 tttttctcgc tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg
    43861 agctggctgt tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg
    43921 ctatgtgcgg cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg
    43981 cccgttcgcg aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg
    44041 acgctggcgg gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc
    44101 aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg
    44161 tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac
    44221 cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc
    44281 ggcctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag
    44341 cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg cagctatatg
    44401 gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg
    44461 ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt
    44521 attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgccgcgcgt
    44581 cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt
    44641 gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac
    44701 tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg
    44761 ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg
    44821 caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa
    44881 ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc
    44941 aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat
    45001 gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt
    45061 aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg
    45121 cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat
    45181 gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca
    45241 agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca
    45301 tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt
    45361 tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat
    45421 tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat
    45481 cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga
    45541 aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt
    45601 ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg aagagccgaa
    45661 taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa
    45721 agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat
    45781 cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt
    45841 gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc
    45901 tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa
    45961 aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg
    46021 cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg
    46081 caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa
    46141 gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt
    46201 cgtgccgtat accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc
    46261 cgtgaccaca gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc
    46321 cgggcacgat ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga
    46381 gctggagcgc ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc
    46441 aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg
    46501 catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga
    46561 catttttgat cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct
    46621 gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg
    46681 tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa
    46741 ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa
    46801 tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg
    46861 tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg
    46921 cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct
    46981 gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct tgccggtcaa
    47041 cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac
    47101 tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata
    47161 aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac cagtatattc
    47221 aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg
    47281 g
//
LOCUS       DADLSE010000015        47036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLSE010000015 DADLSE010000000
VERSION     DADLSE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.015"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN692_000862"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN692_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7263242.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLCFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN692_000863"
     CDS             complement(1614..1958)
                     /locus_tag="JN692_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7263243.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAM
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGTSKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN692_000864"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN692_000864"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7263244.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN692_000865"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN692_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7263245.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN692_000866"
     CDS             complement(6144..6842)
                     /locus_tag="JN692_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7263246.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN692_000867"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN692_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7263247.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN692_000868"
     CDS             7537..8574
                     /locus_tag="JN692_000868"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7263248.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMVANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN692_000869"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN692_000869"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7263249.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN692_000870"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN692_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7263250.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN692_000871"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN692_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7263251.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN692_000872"
     CDS             10745..11626
                     /locus_tag="JN692_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7263252.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN692_000873"
     CDS             complement(11661..12989)
                     /gene="srmB"
                     /locus_tag="JN692_000873"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7263253.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13121..13858
                     /gene="trmN"
                     /locus_tag="JN692_000874"
     CDS             13121..13858
                     /gene="trmN"
                     /locus_tag="JN692_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7263254.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN692_000875"
     CDS             complement(13843..15465)
                     /gene="nadB"
                     /locus_tag="JN692_000875"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7263255.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15721..15876
                     /gene="rseD"
                     /locus_tag="JN692_000876"
     CDS             15721..15876
                     /gene="rseD"
                     /locus_tag="JN692_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7263256.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWCFDSAWKFG
                     LGRLYLG"
     gene            15873..16448
                     /gene="rpoE"
                     /locus_tag="JN692_000877"
     CDS             15873..16448
                     /gene="rpoE"
                     /locus_tag="JN692_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7263257.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16481..17131
                     /gene="rseA"
                     /locus_tag="JN692_000878"
     CDS             16481..17131
                     /gene="rseA"
                     /locus_tag="JN692_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7263258.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17131..18087
                     /gene="rseB"
                     /locus_tag="JN692_000879"
     CDS             17131..18087
                     /gene="rseB"
                     /locus_tag="JN692_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7263259.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18084..18563
                     /gene="rseC"
                     /locus_tag="JN692_000880"
     CDS             18084..18563
                     /gene="rseC"
                     /locus_tag="JN692_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7263260.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18761..20560
                     /gene="lepA"
                     /locus_tag="JN692_000881"
     CDS             18761..20560
                     /gene="lepA"
                     /locus_tag="JN692_000881"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7263261.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20576..21550
                     /gene="lepB"
                     /locus_tag="JN692_000882"
     CDS             20576..21550
                     /gene="lepB"
                     /locus_tag="JN692_000882"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7263262.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21822..22502
                     /gene="rnc"
                     /locus_tag="JN692_000883"
     CDS             21822..22502
                     /gene="rnc"
                     /locus_tag="JN692_000883"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7263263.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22499..23404
                     /gene="era"
                     /locus_tag="JN692_000884"
     CDS             22499..23404
                     /gene="era"
                     /locus_tag="JN692_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7263264.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPIILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23416..24144
                     /gene="recO"
                     /locus_tag="JN692_000885"
     CDS             23416..24144
                     /gene="recO"
                     /locus_tag="JN692_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7263265.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN692_000886"
     CDS             24156..24887
                     /gene="pdxJ"
                     /locus_tag="JN692_000886"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7263266.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVDAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24887..25267
                     /locus_tag="JN692_000887"
     CDS             24887..25267
                     /locus_tag="JN692_000887"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7263267.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25687..25767
                     /gene="shoB"
                     /locus_tag="JN692_000888"
     CDS             25687..25767
                     /gene="shoB"
                     /locus_tag="JN692_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7263268.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFS"
     gene            complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN692_000889"
     CDS             complement(25961..26221)
                     /gene="yfhL"
                     /locus_tag="JN692_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7263269.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(26277..27125)
                     /locus_tag="JN692_000890"
     CDS             complement(26277..27125)
                     /locus_tag="JN692_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7263270.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27334..27969
                     /gene="yfhb"
                     /locus_tag="JN692_000891"
     CDS             27334..27969
                     /gene="yfhb"
                     /locus_tag="JN692_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7263271.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            27994..28530
                     /gene="tadA"
                     /locus_tag="JN692_000892"
     CDS             27994..28530
                     /gene="tadA"
                     /locus_tag="JN692_000892"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7263272.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN692_000893"
     CDS             complement(28527..30083)
                     /gene="mltF"
                     /locus_tag="JN692_000893"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7263273.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYLIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30341..34228
                     /gene="purL"
                     /locus_tag="JN692_000894"
                     /gene_synonym="purI"
     CDS             30341..34228
                     /gene="purL"
                     /locus_tag="JN692_000894"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7263274.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34804..36231
                     /gene="qseE"
                     /locus_tag="JN692_000895"
     CDS             34804..36231
                     /gene="qseE"
                     /locus_tag="JN692_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7263275.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSISFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36396..37109
                     /gene="qseG"
                     /locus_tag="JN692_000896"
     CDS             36396..37109
                     /gene="qseG"
                     /locus_tag="JN692_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7263276.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            37099..38433
                     /gene="glrR"
                     /locus_tag="JN692_000897"
     CDS             37099..38433
                     /gene="glrR"
                     /locus_tag="JN692_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7263277.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38494..38832
                     /gene="glnB"
                     /locus_tag="JN692_000898"
     CDS             38494..38832
                     /gene="glnB"
                     /locus_tag="JN692_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7263278.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN692_000899"
     CDS             complement(38877..40067)
                     /gene="hmpA"
                     /locus_tag="JN692_000899"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7263279.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40394..41647
                     /locus_tag="JN692_000900"
     CDS             40394..41647
                     /locus_tag="JN692_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7263280.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41845..43038)
                     /locus_tag="JN692_000901"
     CDS             complement(41845..43038)
                     /locus_tag="JN692_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7263281.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGELWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43336..46431
                     /locus_tag="JN692_000902"
     CDS             43336..46431
                     /locus_tag="JN692_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HAY7263282.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVANH
                     HIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKRFE
                     QYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNALLD
                     DAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPLAA
                     QDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRADFP
                     QAVAYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSKAG
                     GYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQREK
                     LLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAAEM
                     LNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEECW
                     FARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAARYL
                     DNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLWHL
                     TGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHAAL
                     HYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYYND
                     QPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSLYG
                     DLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFNYV
                     H"
     gene            46528..>47036
                     /locus_tag="JN692_000903"
     CDS             46528..>47036
                     /locus_tag="JN692_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7263283.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINC"
BASE COUNT        11348 a        11854 c        12498 g        11336 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctctgttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgtccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagcatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtctgcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatctcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggctag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatatgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga cggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggta
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa attatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggctgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttagctt ttttcttctc ggcgcgtttt gccagtactt tcttcgatgg cttgccggtc
    11821 tgcttctcgc ttggcgcacg cgttttcggg cgtaactcat caataacgcg agctttaatc
    11881 ggctcttcaa tgtaacggcc aaccttgccc agcagcaaat ggtcatgggc ttccaccagc
    11941 gaaattgcgg tgcctttacg accggcgcgc gcggtacgtc cgatacgatg caaataagta
    12001 tcgccactgc gcggcatatc gaagttaaag acgtggctga cgtcaggaat gtcgataccg
    12061 cgcgcggcaa catcggtggc aaccagtacg tttacgcggc cttcggtcag gcgtttaatt
    12121 gcttcgttac gcttgccttg taccatctca ccttcgagat agcagttgtt gatgcccgct
    12181 tcgcgcagcc agtttgccag ctcatgcaca cgttcacgct tacgcacaaa cacgattgag
    12241 cgagtcgctt caggctgttt taacaggtgc accagcaatg cggttttatg ttccaggtca
    12301 tcggcgcggt aataccactg atgaattttt ttgcgctcac gggtggacgg attggcagaa
    12361 acttccaccg gatcttccag cagacgctcg gcaaagtcct gaatcgcatc gccttccagc
    12421 gttgccgaaa agagcagggt ctgtttacgc cagcgagttt cgccagcaat atgttcgata
    12481 tcctgagcga agcccatatc cagcatacgg tctgcttcgt cgaggatcag cgtttcaacc
    12541 gcgcggcaat cgaagttctc ttcttttatg tattgcagca gacgtccggt cgtggcgacc
    12601 acgatgtcct gattttcgct gaacacttcc gcgtggttca tataggctac gccgccggtg
    12661 atggtggcga tatccagatg cgtatgtttc gccagttcgc gggcatgatc ggccacctgc
    12721 atcgccagct cgcgagttgg ggtgaggatc aaaatacgcg gcggaccgga tttcttacgt
    12781 gggaaatcga gcaggtgctg caacgctggc agcagatacg ccgccgtttt accggtgcct
    12841 gtcggcgcag aaccgagtac atcacggcca tcgagcgcag gcggaatggc ggcagcctga
    12901 atggcggtcg ggcgagtgaa acctttatcc tggagggctt ccagcaggct ttcgtcgagt
    12961 tcaagttcgg aaaaagtcgt tacagtcatg ttctacctct gtgtggggcg ctgattatag
    13021 acgttacggc tgcaatcttc atctgtttgt atggatatcg cttctcgggt attgttctca
    13081 cccggtgcta tcgccgtttt tcctgcaagg ttttattttc atgtcacagt ctacatccgt
    13141 gcttcgtcgt aatggattta cttttaaaca gttttttgtt gctcacgatc gctgtgcgat
    13201 gaaagtggga acggatggca ttttgctggg cgcatgggca ccggtggctg gggtgaaacg
    13261 ttgccttgat atcggcgcgg gtagcgggtt gctggcactg atgctggcgc agcgaaccag
    13321 tgacagcgtg ataattgatg ccgttgagct ggaaagtgaa gctgccgcgc aggcgcagga
    13381 aaatatcaac cagtccccgt gggcagagcg gattaacgtc catacggcgg atattcagca
    13441 gtggatcaca cagcagacag tacgcttcga tttaatcatc agtaacccac cttactatca
    13501 gcagggagta gagtgcgcga cacctcaacg ggaacaggct cgctatacca ctacgcttga
    13561 tcacccatcg ctgctgacct gtgcggcgga gtgcattacc gaagagggat ttttctgcgt
    13621 ggtgctgcca gagcagattg gtaatggttt tactgaactg gcattaagca tgggctggca
    13681 tttacgtttg cggacggatg tggcggaaaa cgaagcgcga ctgccgcatc gggtgctgct
    13741 ggcattctcc ccgcaggcgg gagaatgctt tagcgatcgc ttagtcattc gtgggccaga
    13801 ccagaactat tccgaagcgt atacggcgct gacccaagct ttttatctgt ttatgtaatg
    13861 attgccgggg gaaaggatcg atggaccgga atgcgtgagc agttccgggt aatccagcgt
    13921 aaaatgcagc ccccggcttt ctttgcgcat catcgcgcag cgaacaatca actcggcaac
    13981 ctgtaccaga ttacgcagct ccagcaaatt atttgagacg cggaaatggg cgtaatattc
    14041 gtctatttct tgttggagca tggttatccg ccgcagggcg cgttccaggc gcttcgttgt
    14101 gcgcacaatg ccaacgtaat cccacataaa cagccgtagc tcgtgccagt tatgctgaat
    14161 tactacccgt tcgtcagggt tctcaacgcg gctttcatcc cacggcggta acgtactgac
    14221 gccgtgggca taaggcatac gtctggtgat atcttctgcc gccgaccagc catagaccag
    14281 acactccagc aatgaattcg aggccatgcg gttagcgccg tgtaagccgg tataactcac
    14341 ctcgccaatg gcatacaagc cctcgacgtc cgtacgccca tgatcatcaa ccattacacc
    14401 accgcaggta tagtgtgcag caggcacaat cggtaccggt tcttgtgtga gatcaatccc
    14461 cagcccgagc aatttttcat aaatcatcgg gaaatgctgg cgaataaaat cggcgggctt
    14521 atggctgatg tcgaggaaca tacaatctgc gccgaggcgt ttcatttcat ggtcaatggc
    14581 gcgggcgaca atatcgcgcg gggctagttc gccgcgctca tcaaaatcgg gcataaaacg
    14641 cgtgccatcc gggcgcttga gataagcgcc ttcgccgcgc agtgcttccg ttaacaggaa
    14701 attgcgtgcc tgtgggtgat acagtgcggt agggtggaac tgattaaatt cgagattggc
    14761 aacccgacag cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt
    14821 ggtggtgtac tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg
    14881 gcaggtttcc accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt
    14941 gcccggcagg ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac
    15001 acgaatattc ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt
    15061 ggcgtcggcg gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta
    15121 gctttcttcg ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca
    15181 ttgcacacag gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc
    15241 agccccggca atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac
    15301 ggcggcaata ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag
    15361 aacgatgacc tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct
    15421 accaataatc aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatt
    15481 tactaaacat tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta
    15541 atatttgtta cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg
    15601 gctggtggag gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa
    15661 atgggcattt ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata
    15721 atgatccgtc tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga
    15781 gacactctaa ccctttgctt gctcaaattg cagctaatgg agtggtgttt cgatagcgcg
    15841 tggaaatttg gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc
    15901 tggttgaacg ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc
    15961 agcataaagt ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg
    16021 tacaagaagc ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt
    16081 tttatacatg gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg
    16141 ggcgtcgtcc accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg
    16201 gcgcgttgaa agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga
    16261 tagttttccg aactattgag tccctcccgg aagatttgcg catggcaata accttgcggg
    16321 agctggatgg cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg
    16381 tgcgttcacg tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca
    16441 ggcgttgacg atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc
    16501 cgctttaatg gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc
    16561 agaaatgcag aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac
    16621 tcccgaggtg ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc
    16681 agtacgtcaa ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcagaa
    16741 aatgccattc tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc
    16801 cgcatgcgta tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac
    16861 gtcccagcag cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc
    16921 ggtaagcctg ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca
    16981 ggagcagcgt cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca
    17041 ctctgaacag cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg
    17101 aattcaaact ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta
    17161 gtgacaggta gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta
    17221 ttacagcaga tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc
    17281 aataaacagg gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt
    17341 gcacaattgt tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc
    17401 agctattttg aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct
    17461 ctgccatcgc ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc
    17521 gtcgggcgca cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat
    17581 ggtacacgct acagctacat cgtgtggatg gacaccgaat cgaaattacc gatgcgggtt
    17641 gatctccttg atcgcgatgg tgaaacgctg gaacaatttc gcgtgattgc ttttaacgtc
    17701 aatcaggata tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt
    17761 tctgttcctg taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt
    17821 tttagcgaag tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa
    17881 tcacgtctct attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca
    17941 tcgagcaccg atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat
    18001 aacgccgaaa tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag
    18061 aatattaagt tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa
    18121 acgggcaggc gctggtcagt tgtgatgtta aagcttcatg cagcagctgc gcttcacgcg
    18181 ccggttgcgg tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg
    18241 taccctgtga tgagccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca
    18301 gcctgcttag ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttcctcatcg
    18361 cttcgctatt tcagctactc tttgcttctg acgtcgcagc attatgtggt gcggtcctcg
    18421 gcggtattgg cgggttcctg attgcgcgcg gctactcgcg taagtttgct gcccgggcag
    18481 aatggcaacc gatcatctta agcgtggcat tgccgcctgg ccttgtgcga tttgaaacat
    18541 cttcggaaga cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg
    18601 aggacgtttc cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta
    18661 gtgtagaatg cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa
    18721 gcctggttaa ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt
    18781 ttcgatcata gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat
    18841 ctgcggtggc ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga
    18901 gcgtgagcgt ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga
    18961 cggcgaaacc tatcagctta actttatcga caccccgggc cacgtagact tctcctatga
    19021 agtttcccgt tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg
    19081 cgtagaagcg caaaccttgg caaactgcta caccgccatg gaaatggatc tcgaagtggt
    19141 gccggtactg aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat
    19201 tgaagatatc gtcggcatcg acgccaccga tgcagtgcgc tgttcagcga aaaccggcgt
    19261 tggcgtgcag gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc
    19321 ggaaggcccg ttgcaggcac taattatcga ctcctggttc gacaactacc tgggcgttgt
    19381 ttcacttatc cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag
    19441 taccgggcag acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg
    19501 cactgaactg aaatgtggcg aagtaggctg gctcgtatgt gcgattaaag atatccacgg
    19561 cgctccagtc ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg
    19621 ctttaagaaa gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta
    19681 tgaagccttc cgtgacgcgc tgggtaaact cagcctgaac gatgcctcac tgttctatga
    19741 gccggaaagc tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca
    19801 catggagatc atccaggagc gtctggaacg tgaatacgat ctggatctga tcaccactgc
    19861 gccgaccgta gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc
    19921 atccaagctg cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca
    19981 catgctgctg ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg
    20041 cgtgcagacc aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat
    20101 ggcggaagtg gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc
    20161 tctggattac aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat
    20221 caacggtgaa cgtgttgatg cgctggcgtt gatcacccac cgtgataatt cgcaaaaccg
    20281 cggtcgcgag ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc
    20341 cattcaggca gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa
    20401 aaacgtactg gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa
    20461 gcagaaagaa ggtaagaaac gcatgaagca gatcggtaac gttgagctgc cgcaggaagc
    20521 gttcctcgcc attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc
    20581 gaatatgttt gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt
    20641 ggataaattc tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc
    20701 cggtgactca ctggataaag caacgttgaa aaaggttgca ccgaagcctg gctggctgga
    20761 aaccggagct tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta
    20821 tgaaccgttc cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat
    20881 tctggtagag aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga
    20941 aaccggtcat ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct
    21001 tgattacatc aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc
    21061 aaaagagctg acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc
    21121 ggtcacctac tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg
    21181 tggggaagcg accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat
    21241 tcgtctttcc gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc
    21301 gattgcgcag gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg
    21361 gattgttccg ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag
    21421 ccgttactgg ggctttgtgc ctgaagcgaa tctggtcggt cgggccacgg ctatctggat
    21481 gagcttcgat aagcaagaag gcgaatggcc gactggtgtg cgcttaagtc gcattggcgg
    21541 catccattaa tagccatctt cgttcacgtt gtcgccgtta tggcgacaac gtgaattatt
    21601 tatgagataa atctcccgtg gctaacgaca tcccccgtcg ttgtgtatag aatattcccc
    21661 cgaagtttaa ggttggcacc tccaggttgc cacggcacac gaaacagcgt tggtccccat
    21721 ataccgctaa actgaaactg cagcgaagca gttagcagag ccatgtatat caggtctgtt
    21781 tcgtgtgctg aattgttgac gcatttattt attggtatcg catgaacccc atcgtaatta
    21841 atcggcttca acggaagctg ggctacactt ttaatcatca ggaactgttg cagcaggcat
    21901 taactcatcg tagtgccagc agcaaacata acgagcgttt agaattttta ggcgactcta
    21961 ttctgagcta cgttatcgcc aatgcgcttt atcaccgttt ccctcgtgtg gatgaaggcg
    22021 atatgagccg gatgcgcgcc acgctggtcc gtggcaatac gctggcggaa ctggcgcgcg
    22081 aatttgagtt aggcgagtgc ttacgtttag ggccaggtga acttaaaagc ggtggatttc
    22141 gtcgtgagtc aattctcgcc gacaccgtcg aagcattaat tggtggcgta ttcctcgaca
    22201 gtgatattca aaccgtcgag aaattaatcc ttaactggta tcaaactcgt ctggacgaaa
    22261 ttagcccagg cgataaacaa aaagatccga aaacgcgctt gcaagaatat ttgcagggtc
    22321 gccatctgcc gctgccgact tatctggtag tccaggtacg tggcgaagcg cacgatcagg
    22381 aatttactat ccactgccag gtcagcggcc tgagtgaacc ggtggttggc acaggttcaa
    22441 gccgtcgtaa ggctgagcag gctgccgccg aacaggcgtt gaaaaaactg gagctggaat
    22501 gagcatcgat aaaagttact gcggatttat tgccatcgtc ggacgtccga acgttggcaa
    22561 atccacattg ttgaacaaac tgctggggca gaaaatctcc atcacctccc gcaaggcgca
    22621 gacaactcgt caccgcattg tggggatcca tactgaaggc gcgtatcagg cgatctacgt
    22681 cgatacaccg ggcctgcata tggaagaaaa acgcgccatt aaccgcctga tgaacaaagc
    22741 ggcgagcagc tctattggcg atgttgagct ggtgattttt gtcgttgaag gcacccgctg
    22801 gacgccggac gacgaaatgg tgctcaacaa actgcgcgaa ggcaaagcgc cgataatcct
    22861 cgcggtgaac aaagtggaca acgtgcagga gaaagccgat ctgctgccgc acctgcagtt
    22921 cctggcaagc cagatgaact tcctcgatat cgtgccaatc tctgccgaaa ccgggctgaa
    22981 tgttgacact attgcggcaa tcgtgcgtaa gcatctgcct gaagcgactc atcacttccc
    23041 ggaagattac atcaccgatc gctcacagcg ttttatggcg tctgaaatca tccgcgaaaa
    23101 actgatgcgt ttccttggcg ctgaactgcc gtactccgtg accgtggaga tcgaacgttt
    23161 cgtctctaac gaacgcggcg gttatgacat caacggtttg attctcgttg agcgtgaagg
    23221 gcagaagaag atggttattg gcaacaaagg ggccaagatc aaaaccatcg ggattgaagc
    23281 gcgtaaagac atgcaggaaa tgttcgaagc gcctgtccac cttgaactgt gggtaaaagt
    23341 gaaatcgggt tgggccgacg acgaacgcgc actgcgcagt ctcggttatg ttgacgatct
    23401 ttaagagtca ctccgatgga aggctggcag cgcgcatttg tcctgcatag tcgcccgtgg
    23461 agcgaaacca gcctgatgct ggacgtcttc acagaggaat cgggtcgcgt gcgtctggtt
    23521 gccaaaggcg cacgctctaa acgctctact ctgaaaggtg cattacagcc tttcacccct
    23581 ctcttgctac gttttggcgg gcgtggcgaa gtcaaaacgc tgcgcagtgc tgaagctgtc
    23641 tcactggcgc tgccattaag cggtatcacg ctttacagcg gtctgtacat caacgaactt
    23701 ctctcccgcg tactggaata cgagacgcgc ttctctgaac tctttttcga ttacttgcac
    23761 tgcattcagt ctcttgcagg tgccactggt acgccagaac ccgcgctgcg ccgctttgaa
    23821 ctggcactac tcgggcatct gggctatggc gtcaatttta cccattgcgc gggtagcggc
    23881 gagccggtag atgacaccat gacgtatcgt tatcgcgaag aaaaagggtt tatcgcaagc
    23941 gtcgttatcg acaataaaac gttcaccgga aggcagttaa aagcgttaaa cgcgcgggaa
    24001 tttcctgacg cagacacact acgcgccgcg aaacgcttta cccgaatggc gcttaagccg
    24061 tatcttggcg gtaaaccatt aaagagcagg gaactgttcc ggcagtttat gcctaagcga
    24121 acggtgaaaa cacattatga atgatgagga ttgtcatggc tgaattactg ttaggcgtca
    24181 acattgacca tattgcaacg ctgcgtaacg cacgcggtac tgcttacccg gatccggtgc
    24241 aggcggcgtt tattgccgag caggcgggag cggacggcat taccgtgcat ttacgtgaag
    24301 atcgccgcca cattactgac cgcgatgtgc gcatcctgcg gcagacgctg gatacccgaa
    24361 tgaatctgga gatggcagtg accgaagaga tgctggcgat cgccgttgag acgaagccac
    24421 atttttgctg cttggtaccg gaaaagcgtc aggaagtaac aaccgaaggc ggcctggacg
    24481 ttgcagggca gcgtgaaaaa atacgcgatg cctgcaaacg tctggcagat gccgggattc
    24541 aggtttctct gtttatcgac gccgatgaag agcagatcaa agctgcggca gaggttgacg
    24601 caccgtttat cgagatccac accggttgct atgctgatgc caaaactgac gccgaacagg
    24661 cgcaagagct ggtgcgtatc gccaaagccg cgacctttgc cgctagtctc ggtctgaaag
    24721 ttaacgccgg acacggtctg acctatcaca acgtgaaagc cattgccgcc atccctgaga
    24781 tgcatgaact gaatatcggt catgccatta ttggccgtgc agtgatgacc ggactgaaag
    24841 atgcggtggc agaaatgaag cgtctgatgc tggaagcgcg tggctaatgg caatattagg
    24901 tttaggcacg gatatcgtag agatcgctcg catagaagca gtgatcgccc gatccggtga
    24961 gcgcctggca cgccgcgtat taagcgataa cgaatgggca atctggaaaa cgcaccacca
    25021 gccggtgcgt tttctggcga agcgttttgc tgtgaaagaa gccgcagcaa aagcgtttgg
    25081 caccgggatc cgcaatggtc tggcgtttaa tcaatttgaa gtattcaatg atgagctcgg
    25141 caaaccacgg ctacggctat ggggtgaggc attaaaactg gcggaaaagc tcggcgttgt
    25201 aaatatgcat gtaacgctgg cagatgaacg ccactatgct tgtgccacgg taattattga
    25261 aagttaaaat aataccgttc tctttaaaaa gtaaaacccc cgaactggta aggaacgggg
    25321 gttttacttt ttaactttaa ttttgtgcag catgcaccct caacaacagc gattatggat
    25381 cagtgtactt atcttttaca agcaacttgc aatctttagc ataaaaactc gagcctttac
    25441 gaagaaagca atattgatgg aaagattaac gtgaccgcca attcgtaagt acattaaaat
    25501 tggcttcgtt attgaagatt ttgctgtgct ttacaccatg ccacagaatt cccccattga
    25561 aacgagtggt gtcgtcaaag ctctggtgtg gagtgcagca tgcaccctca ataactcgca
    25621 cgttcagttt tggggagatg taagggctaa tctgaatggc tgcattcctt gtttaaggaa
    25681 aaacgaatga ctgattgccg atacctgatt aaacgggtca tcaaaatcat cattgctgtt
    25741 ttacagctga tccttctgtt ctcataacac aaggaaacgt acttaaggtg cgtccggtga
    25801 accaatcgga cgcgccttta ataactataa ataagtgtct tggcagatac tatataaatt
    25861 aacttagtga atgattatgc taatgtcatc aattaaataa atataatggc gttaaggctt
    25921 cccagtaata taattaatac tctacttcca gagtagaata ttaaatttta tccgcgtggt
    25981 gcatcagcac aaatttatcc cacaactgtt cttctgtctc gacatgcgcc ggatctttca
    26041 caatagtatt ggggatcggg cacaccttct ggcaggttgg tgtctcgtag tgccctacgc
    26101 attcggtaca cttatcgctg ttaatctcgt agatatgatc tcccattgaa atcgcctcat
    26161 tcgggcattc gggttcacac atatcacaat tgatgcattt tttagtgatt aacagcgcca
    26221 taaggaaact ctcaaaacaa cacaaaccgg gcgggcatta tacgcgcatt ctttactcag
    26281 accagttttt tcaccagcgc ttcactatga cgaatacgtt ctggtgccag ttccagatcc
    26341 tgctgaatca gcgcaatgaa cagcaaatcc gttaacattc cctgagcgtg acaagcagag
    26401 attgaagcac tgtttgtcgc ttgttcttcg gcaatggtat acaggcaatg agaagcacgc
    26461 tgttgcaggg cattcggagt aaagccggta atcgccagca cttttccgcc cactcgcagc
    26521 atctcatctg ccgccaggtt taactcgcgt cgtacaccgg tgtaggaaat ggctaataac
    26581 agatcgtcag gggacgacgc ctgtactgtt gcgagtagcg catgcatatc gcgcagtgcg
    26641 gcagcattga agccaatctt catcagcttc caggcaaagt tttgcgccac cagacccgaa
    26701 gcgccaatac cggtcagaat tatccgccgc gcagagcgca acattgttac gcattcatgc
    26761 agtttctctt cactattaac gtttagcgtt gcgtacatag cggcagtatt ttctttaatc
    26821 agtttttcgc cgaccagccg taacggatca tcaccgcgga tttggttatg aatgggcacg
    26881 gagggcgatt ccggctggct tgccagcgct tcactcaacg ccaacttaag cgccggaaaa
    26941 cctttatagc cgagtttttg cgcgaacttc acgacgctgg actgactgac tccggcttcg
    27001 ttggccagtt gctgagagct taaatggcgc gccgtatcag gttgtagcag cagataatcc
    27061 gccagttttt tatcactctg ggcaagcccc tggtaacgct gacggatacg aagtaagccg
    27121 ttcatatctt tcctcgcgat gaaatagtgg cgacaacagg caatatgaga cacacgcgag
    27181 tgaattttat attccattgc cgctaattgc catgaattaa aaattccagt tagccatgat
    27241 gcggtcgatt gttgccggaa aagtgatgat ctatctgtta ggctatgtag tagcgaaaat
    27301 taatcaggat gtttcagtcc agaggagtat ggtttggcaa ctcacgagcg tcgtgtggtg
    27361 ttttttgact tagatggaac attgcatcag caggatatgt tcggcagttt tctgcgctat
    27421 ttactgcgtc gccaaccgct gaatgcgtta cttgtcctac cgttgttacc gattatagcc
    27481 attgcgttat tgataaaagg tcgtgcggca cgctggccga tgagtctgct tctgtggggg
    27541 tgcacttttg gtcacagcga agcacgttta cagacgttgc aggccgattt cgtgcgctgg
    27601 tttcgcgaca atgttaccgc ctttccgctg gttcaggagc gattaaccac ctacctgtta
    27661 agttccgatg ctgatatctg gttgattacc ggctctccgc agccgctggt tgaagcggtt
    27721 tatttcgata cgccctggct gccgcgggtt aatcttatcg ccagccaaat tcagcgtggc
    27781 tatggtggtt gggtattgac gatgcgttgt ctgggacatg aaaaggtcgc acaactggag
    27841 cgcaaaatcg gcacgccgct gcggctgtac agtggctata gcgacagtaa tcaggacaat
    27901 ccgctgcttt atttctgtca gcatcgttgg cgagtaaccc cgcgcggtga actccagcaa
    27961 ctggaataga gtaaagcata gcgtccgtgt ataatgcgcc gcgcttttat aaccggagtt
    28021 ttctttttgt ctgaagtcga atttagccac gaatactgga tgcgtcacgc gctgacgctg
    28081 gcgaaacgtg cctgggatga gcgggaagtg ccggtcggcg cggtattagt gcataacaat
    28141 cgggtaatcg gcgaaggctg gaaccgcccg attggtcgcc atgatcccac cgcacatgca
    28201 gaaatcatgg ccctgcggca gggtggtctg gtgatgcaaa attatcgtct gatcgacgcc
    28261 acgttgtatg tcacgcttga accatgtgta atgtgtgccg gagcgatgat ccacagtcgc
    28321 attggtcgcg tggtctttgg tgcgcgtgac gcgaaaactg gcgctgcggg atctttaatg
    28381 gatgtgctgc atcatccggg tatgaatcac cgagtggaaa ttacggaagg aatactggcg
    28441 gatgagtgcg cggcgttgct cagtgacttc tttcgcatgc gccgccagga aattaaagcg
    28501 cagaaaaaag cgcaatcctc gacggattaa ttttgtttct cttcactccc tttcctggaa
    28561 aacagcagag agggagaatg ggtcaaatag tttgatgacg actgggaaag aaacgagaga
    28621 aaaggaaatt tctctttgcc caactccgta ggcgataccg ccggataatc ctgcgccagt
    28681 tgcatcgccg cttctgtagc ctgcttctct ttctcttgca gataacccac caggctaatc
    28741 agatacttac gaatattttc gacataagcg taggcttcat gcccacgagc gtagccgtaa
    28801 gtcagcttgc tgtaataggg tttctggcta agtaaaggca gacgctgttt tacgtcagcc
    28861 caactgtcag gattcccttt ggtttttgcc gtcagggcgc gggcatccag catatgcgca
    28921 tagcccatat tgtacgcagc gagggcaaac cagatccgtt cgttctccgg cacactttcc
    28981 ggcactttac tcatcatatc ctgcaaataa cgcacgccac cgctgatgct ctgttcggca
    29041 tcggtacgat ccgtaatgcc gaggctttgc gcggtatttt tggttaacat catcatgccg
    29101 cgcacacccg tcggtgaagt ggcctgtgca tcccagtgcg attcctgata agcaatagcg
    29161 gccagcaaac gccagtcaat ttcttcggcg tatttctcaa acaggggctt taactgcggc
    29221 agtaccgcat cgacggcgcg taaaaatgtg cgcgtatcga cgtaatcaaa atcatcgcca
    29281 tgccccaggt atttctcttc aatgcgtgcc agcgtaccgt cttcattcat ttcgttgaag
    29341 aagtcgagca gggcggcgga aagggtatta tcgccatcta acgggctaaa ccaagtcacc
    29401 ggttgttcat cggtgatatc gagcgctacg gcgagctccg ggtgaacgcg ctgaaacagg
    29461 ctgatggcga cagaatcagc aatggtgtaa tcgagttttc cttcgatgac atcttccatt
    29521 aattccgcag agcctttttt gtcgtctacc ttccagctta attccgggaa ttttgtttct
    29581 ttcagggtct ggagatcgtt aaccaccaca tgacccggtg caacggtaag ttgctccgcc
    29641 gtcaggttgc ccagcgtacg tgggcgatac tgacccactt tataaaccag ttgttgtgac
    29701 acggaataat aggtagggcc aggctgataa ttttttaccc gctcactgtt atagaccagt
    29761 cctgccgcca gcaggtcggc gttaccatta tcaaggtcgt caaacagctg gctgatattc
    29821 tgccgcacgg tcactttcag ttttacgccg aggtaatcgg caaactgttt cgccagttcg
    29881 taatccaggc caaaaggttt cccgttgatt tcgttataag tcaggggagt atgaatggtg
    29941 ctcacacgca actctccccg cgcttgaatg gcggcgatac ggttgtcggc tttaccaaac
    30001 cagggaatgg atggccagag agcgaccgcg agcagcagtg ccagaatgcc gatgaacaga
    30061 taattaatct ttaatttttt caattagtta attctctgtg tcgtgcgcgt cccagcttga
    30121 aaaaacgtaa taatagtgaa aggtttactc ataaatgagc ggcattttgc gtaaacctgc
    30181 gccagatggc aacttattac agccattggc ggcacgcgtt gctaattcac gatggtgatt
    30241 ttatttccac gcaaacggtt tcgtcagcgc atcagattct ttataatgac gcccgtttcc
    30301 cccccttggg tacaccgaaa gcttagaaga cgagagactt atgatggaaa ttctgcgtgg
    30361 ttcgcctgca ctgtcggcat tccgaatcaa caaactgctg gcacgttttc aggctgccag
    30421 gctcccggtt cacaatattt acgccgagta tgtccatttt gctgacctca atgcgccgtt
    30481 aaacgatgat gagcacgcac aacttgaacg cctgctgaaa tatggtccgg cactcgccag
    30541 ccacgccccg caaggcaaac tcctgctggt gaccccgcgt cctggcacca tctccccctg
    30601 gtcttcgaaa gcgaccgata ttgcccataa ctgcgggcta caacaggtaa accgccttga
    30661 gcgcggcgtt gcttactata tagaagccgg tacgctgacc aatgaacaat ggcagcaggt
    30721 taccgctgaa ctgcacgacc gcatgatgga aacggtcttt tttgatttag atgatgcaga
    30781 gcagttgttt gcccaccatc aaccgactcc ggtcacaagc gttgatttgc tggggcaggg
    30841 ccgtcaggcg ctgatcgacg ctaacctgcg tcttggcctg gctctggcgg aagatgaaat
    30901 tgactatctg caggatgctt tcacaaagct tggtcgtaac ccgaacgaca tcgagctgta
    30961 tatgttcgct caggcgaact ctgagcactg ccgccacaaa atttttaacg ccgactggat
    31021 tatcgacggt gaacaacagc cgaaatcgct gttcaggatg atcaaaaata ctttcgaaac
    31081 cacgccagat cacgttctct ctgcttataa agataacgcc gccgtaatgg aaggttctga
    31141 agtgggccgc tactttgctg accacgaaac gggccgctac aatttccatc aggaaccggc
    31201 gcacatcctg atgaaagtcg aaactcacaa ccacccgacg gcgatttctc cgtggccggg
    31261 cgcggcgacc ggttccggcg gtgaaatccg cgatgaaggt gccaccgggc gcggcgcgaa
    31321 gccgaaagct ggtttggttg gtttctccgt atccaacctg cgaattcctg gcttcgaaca
    31381 gccgtgggaa gaagatttcg gtaagcctga gcgcattgtt accgcgctgg acatcatgac
    31441 cgaaggcccg ctgggcggtg cggcgtttaa caacgaattt ggtcgtccgg cactgaacgg
    31501 ctacttccgt acttatgaag aaaaagtgaa cagccacaat ggcgaagagc tgcgcggtta
    31561 tcacaaaccg atcatgctgg cgggcgggat cggcaacatt cgcgccgatc acgtacaaaa
    31621 aggcgagatc aacgtcggtg cgaagctggt cgttctcggc ggcccggcaa tgaacatcgg
    31681 tcttggcggc ggcgcggcgt cttccatggc gtctggtcag tctgatgccg acctcgactt
    31741 tgcttccgta cagcgcgaca acccggagat ggagcgtcgt tgccaggaag tgatcgaccg
    31801 ttgctggcag cttggtgatg ctaacccaat tctgtttatc cacgacgtcg gtgcgggcgg
    31861 tctttctaac gcgatgccgg agctggtgag cgacggcgga cgcggcggta aatttgaact
    31921 gcgcgatatt ctaagcgacg aaccgggcat gagcccgctg gaaatctggt gtaacgaatc
    31981 ccaggaacgc tacgtgctgg cggttgctgc cgatcaatta ccgctgtttg acgaactgtg
    32041 taagcgtgag cgcgcaccct acgcggtgat tggtgaagcg accgaagaac tgcatctttc
    32101 tctgcacgat cgtcattttg ataatcagcc gatcgatctg ccgctggacg tcctgcttgg
    32161 taaaacgccg aagatgaccc gcgatgtaca aacgctgaaa gcgaagggcg atgcgctggt
    32221 ccgtgaaggg atcaccattg cagacgcggt gaaacgcgta ctgcatctgc cgaccgtggc
    32281 ggagaaaact ttcttggtga ccatcggtga ccgcagcgta accggcatgg tggcgcgcga
    32341 tcagatggtg gggccgtggc aggtgccggt cgctaactgc gcggtcacta ccgccagcct
    32401 cgacagctac tacggtgaag cgatggcgat tggcgagcgt gcgccggttg cgctgctgga
    32461 tttcgccgcc tctgcccgtc tggcggtcgg tgaagcgtta accaatattg ccgcaacaca
    32521 gattggcgat atcaaacgca ttaaactttc cgccaactgg atggcggcgg caggccaccc
    32581 tggtgaagat gcgggcctgt atgaagccgt taaagccgtg ggtgaagaac tttgtccggc
    32641 gctgggcctg acgatcccgg tgggtaaaga ctctatgtcg atgaaaaccc gctggcagga
    32701 aggtaacgaa gagcgcgaaa tgacgtcgcc gctgtcgctg gtgatttctg catttgcccg
    32761 cgtggaagat gtacgtcaca ccatcacgcc gcagctttgt accgaagata acgcactgct
    32821 gctgattgat ttgggcaaag gcaataacgc gctgggcgca acggcgctgg cgcaggttta
    32881 tcgtcagctt ggcgacaaac cggcagatgt acgcgatgtt gcccaactga aaggcttcta
    32941 tgacgcgatt caggcgctgg ttgcacagcg taagctgctg gcgtatcacg accgctctga
    33001 tggcggcctg ctggtaacgc tggcggaaat ggcctttgct ggtcattgtg gcattgacgc
    33061 ggatatcgcc tctctgggtg acgatcgcct ggcggcgttg tttaacgaag aactgggtgc
    33121 ggtgattcag gttcgtgccg ctgaccgtga agcggtcgag tccgtactgg cacagcatgg
    33181 gcttgcggat tgcgtccatt atgttgggca ggcggtttcc ggtgaccgtt ttgtgattac
    33241 cgccaacggg cagactgtat tcagcgaaag ccgcactacg ttgcgtgtct ggtgggcaga
    33301 aactacctgg cagatgcagc gcctgcgtga caacccggag tgtgccgatc aggagcatca
    33361 ggcgaaatct aacgacgccg atccgggcct gaatgtaaaa ctgtcgttcg atatcaacga
    33421 agatgtggca gcaccgtata ttgccactgg cgcacgtccg aaagttgccg tactgcgtga
    33481 gcagggcgtg aactcgcatg ttgaaatggc ggcagctttc caccgtgggg gctttgatgc
    33541 tatcgacgtg catatgagcg acctgctgac tggacgtacg ggactggagg attttcacgc
    33601 cctggtcgcg tgcggaggtt tctcctacgg tgatgtgctg ggtgccggtg aaggttgggc
    33661 gaagtctatc ctgttcaatg accgtgtacg cgatgagttt gcaaccttct tccatcgtcc
    33721 gcaaacgctg gccctggggg tatgtaacgg ttgccagatg atgtctaacc tgcgtgaact
    33781 gatcccgggg agcgagttgt ggccacgttt tgtgcgtaac acctccgatc gctttgaagc
    33841 gcgtttcagc ctggttgaag taacccaaag cccgtctctg ctgttgcagg ggatggtggg
    33901 ctcgcaaatg ccgattgctg tctctcatgg tgaagggcgc gtggaagtgc gtgatgcggc
    33961 gcatctggcg gcactggaaa gcaaagggct ggtggcactg cgctatgtcg ataacttcgg
    34021 caaagtcact gaaacctacc cggctaaccc gaacggttca ccgaacggta ttacggcggt
    34081 tacgactgaa agtggtcgag tcaccattat gatgccgcac ccggaacgtg ttttccgtac
    34141 tgtcagcaac tcctggcatc cggaaaactg gggcgaggat ggcccatgga tgcgcatttt
    34201 ccgcaatgcg cgtaagcagt tggggtaagt catcagttca ttggttttgc agcccggatg
    34261 cggcatttac gccgcatccg gcattaagca ccaaccacta tcataagctc ccaactccgg
    34321 gagctttttt gtgtctgtaa atcacgacaa tgggtggttt accgtgtcgc tttctggcga
    34381 cacttaaatg attgatttta ttgtaatcta ataagtttat cttaagttgt ctctttttag
    34441 cgacacagtg gctgatttac attcaaatag cgaagaagcc ataaatccct caaagcaacg
    34501 tataatcagt acgttaccaa actattttct ttattggcac agttactgca taatagtaag
    34561 cagtggctca ttcaccgact tatgtcagcc ccttcgagac gtgctacata aaattcgaat
    34621 gacgcacaac aaggtgcctg ccgtccaact tctgatatca gcgtagctat atcaaccatc
    34681 gggcgaaacg tcgagttagg caccgcctta ttccataaca aagccgggta attcccggct
    34741 ttgttgtatc tgaacttccc ctcggttagc atcaggctat tcgcgtctga cgagagtaac
    34801 accttgaaac gctggcccgt ttttccccgc tcattacgac aactggtaat gctggcattt
    34861 ttgctaattc tactacccct gttggtgctg gcatggcaag cctggcaaag cctgaatgcg
    34921 cttagcgatc aggcggcgct ggttaaccgc actacgctta ttgatgcccg gcgtagtgaa
    34981 gcgatgacca acgcggcgct ggagatggag cgtagctacc gtcagtattg cgtgctggac
    35041 gaccctacgc ttgcgaaggt ttatcaaagc cagcgcaagc gttacagcga aatgctcgat
    35101 gcccacgcag gcgtactgcc agacgataaa ctttaccagg cattgcgtca ggatttgaac
    35161 aatctggccc aacttcagtg taacaacagt ggtcccgatg ctgccgccgc cgcgcgtctt
    35221 gaagcctttg ccagtgccaa taccgaaatg gtgcaggcca cgcgcacagt ggtgttctct
    35281 cgtgggcagc aacttcagcg tgaaatcgcc gaacgtgggc aatattttgg ttggcaatcg
    35341 ctggtgctat ttctggtgag tctggtgatg gtgctacttt tcacgcggat gattatcggg
    35401 ccggtgaaaa atatcgagcg catgatcaac cgtctggggg aagggcgttc tctgggcaat
    35461 agcatttcgt tctgtggacc gagcgagtta cgctcggttg ggcaacgtat tctttggtta
    35521 agtgagcgcc tgtcatggct ggaatcccaa cgccatcaat ttttaagaca tttatctcat
    35581 gaattaaaaa cgccactggc gagtatgcgc gagggcactg aattactggc tgatcaggtt
    35641 gtcgggccgc ttacgccaga gcaaaaagag gtggtgagca ttcttgatag cagcagccgc
    35701 aatttgcaaa aactgatcga acaactgctt gattacaacc gtaaacaggc ggacagtgcg
    35761 gtggaactgg agaatgttga attagcaccg ctggtggaga cagtggtttc tgctcatagc
    35821 ctgcccgcac gggctaaaat gatgcatacc gacgtcgatc tcaaagcaac agcttgcctg
    35881 gcggagccaa tgctgctgat gagcgtactg gataatcttt actccaatgc ggtgcactac
    35941 ggggctgaat ccggtaacat ttgccttcgc agcagtttac atggtgcgcg ggtttatatt
    36001 gatgtcatca atacaggcac gcccattccg caagaggaac gcgccatgat cttcgaaccc
    36061 ttttttcagg gaagccacca gcgaaaaggg gcggtgaagg gcagcggtct gggattaagc
    36121 attgccaggg attgtattcg ccgtatgcaa ggggaactgt atctggtcga cgagagcggg
    36181 caagacgttt gtttccgcat tgaattaccg tcgtcgaaaa acacgaaata atgctaacag
    36241 cctatcctat caggctattt tatttgtcag ttcggccccg ggtagtgctc gaaatcttca
    36301 cgtacgatat gtacgctccg gtttctccgc gctgtccatg tccagactgt ctgcaacaat
    36361 tacgcccact gggataggct ttaagtctgg tgaatatgcg acacattttt caacgattat
    36421 tgccccgacg gctgtggctg gcagggctgc catgtctggc attgctgggt tgcgtgcaga
    36481 atcacaataa gccagccatt gatacgccag cagaagaaaa aattccggtt tatcaactgg
    36541 ctgactatct ttctactgaa tgtagcgaca tctgggcgct gcaaggtaaa tcaaccgaaa
    36601 ccaatccgct ttactggctg cgggcgatgg attgtgctga tcgtttaatg cctgcgcagt
    36661 cgcgtcagca agctcgccaa tatgacgacg gcagctggca aaataccttt aaacaaggga
    36721 ttttgctcgc cgacgccaaa attacgccgt atgagcgccg ccagctggtc gcgcgtattg
    36781 aagcgttaag taccgagatc ccggcacagg ttcgcccact gtatcaactc tggcgcgacg
    36841 gtcaggcgct gcaactgcaa ctggcggaag agcgccaacg ttacagtaaa ctccagcagt
    36901 ccagtgacag cgagctggat acattgcgcc agcaacatca cgttctacag cagcaactgg
    36961 aactcaccac ccgcaagctg gaaaacctga ccgatattga acgccagctc tcgacccgca
    37021 aaccggctgg aaatttctcg ccggatacgc cgcacgagag tgaaaagccc gcgccatcca
    37081 cccatgaggt cactcctgat gagccataaa cctgcgcatt tattattggt cgatgacgat
    37141 ccgggattgc tgaaactgct tggcctgcgc ctgaccagcg aaggctacag tgtggtcacg
    37201 gcggaaagtg gcgctgaagg attacgggta ctgaatcgcg aaaaagtaga tttagttatc
    37261 agcgacctgc ggatggatga aatggacggt atgcagctat ttgctgaaat ccagaaagtg
    37321 cagccgggaa tgccagtaat tattcttact gcgcatggtt ctatccccga tgccgttgct
    37381 gcaacacagc agggcgtttt tagtttcctc accaagcctg tcgataaaga tgcgctatat
    37441 caggcaattg acgatgcgct ggagcaatcc gcgccagcca ccgatgaacg ctggcgcgag
    37501 gcaattgtca cccgcagccc gctgatgctg cgtttgctgg aacaggcgcg gctggtggcg
    37561 caatcagacg tcagcgtttt gattaacggt cagagcggca ccgggaaaga gattttcgcc
    37621 caggctatcc acaacgccag cccgcgcaac agcaaaccat ttattgctat taactgtggc
    37681 gcattgcccg agcaattgct ggagtcggag ctgtttggtc atgcgcgtgg cgcgtttact
    37741 ggcgctgtca gcaatcgcga aggtttattc caggcggcgg aaggcggaac gctatttctc
    37801 gatgaaattg gcgatatgcc tgcgccgtta caggtcaaac tgctgcgcgt gttgcaggag
    37861 cgtaaagtgc gcccgctggg cagtaaccgc gatattgata tcaatgtgcg gattatttct
    37921 gccactcacc gtgatctgtc aaaagcgatg gcgcgcgggg aattccgcga agatctctat
    37981 taccgcctca acgttgtcag cctgaaaatt ccggcactgg cggagcgcac agaagacatt
    38041 ccgctactgg caaatcacct gttgcgccag gcggcagagc gacataaacc gtttgtccgc
    38101 gcgttctcta ccgatgcgat gaaacgcctg atgaccgcga gctggccggg taatgtgcgc
    38161 cagttggtca acgtgattga acagtgcgtg gcgctgacct catctccggt gattagtgat
    38221 gcgctggtgg agcaggcgct ggagggtgaa aatacggcgc tgccaacttt tgttgaggca
    38281 cgtaatcatt ttgaactcaa ctatttgcgt aagctgctgc aaatcaccaa aggcaacgtc
    38341 acccacgcgg cgagaatggc ggggcgcaac cggacagaat tttataaact gctttcccga
    38401 cacgagctgg atgcaaacga tttcaaggaa tgaattggcg ttatgtgtta cgtttagcag
    38461 atcaaaagac aggcgacctt ttcaaggaat agcatgaaaa agattgatgc gattataaaa
    38521 cccttcaagc tggacgatgt ccgcgaagca ctggccgaag tcggtattac cggcatgacg
    38581 gtgaccgaag tgaaaggctt tggtcgccag aaaggccata ccgagctgta ccgcggcgcg
    38641 gagtatatgg tggattttct gccgaaagtg aaaattgaga ttgtcgtacc ggacgacatt
    38701 gtcgatacct gtgtcgatac cattattcgc acggcgcaaa ccggcaaaat cggtgacggt
    38761 aaaatcttcg tctttgacgt ggctcgggtc attcgcatcc gtaccggtga agaggacgac
    38821 gcggcaattt aaaccgcgtc aagggttgcc ggatgtttcc atccggcaac atcaaattac
    38881 agcaccttat gcgggccaaa gcattcgtaa tgaatgtttt cctgcttcac gcccagatcc
    38941 actaactgtt tcgcggtaaa ctgcatgaag ccaaccgggc cgcagagata gaactgcatt
    39001 gtcggatcgc tgaacgcacc ttccagtttg ctcaaatcca tcagaccttc gctatcaaac
    39061 tgacctttag cgcgatcggc ttcgctcggc tgacgatacc aggtgtgcgc ggtaaagcgc
    39121 ggcagtgact gccccagttc cttaacttca tcagcaaagg cgtgaacttc gccattttct
    39181 gccgcatgga accagttcac ttgtgctgtg tggcctgctt ttgccagcgt gtcgagcatt
    39241 gccagcattg gcgtttgacc aacaccggta gagattaacg tcactggtgt gtcatctgcg
    39301 acagccataa agaaatcacc tgccggagcg accagtttca cgacatcgcc aacattggcg
    39361 tgattgtgca accagttgga tacctgccca ccctcttcgc gtttcaccgc aatacgatag
    39421 cctttgccat ccggtttgcg agtcaaagag tactgacgaa tttcctgatg cgggaaacct
    39481 tccggcttca gccagacgcc gagatattgc cccggacggt attctgccac tgcgccaccg
    39541 tcgaccggct ccagttcgaa gctggtgata agcgcgctgc gcggtgtttt agccacaatg
    39601 cggaaatcgc gagtaccttc ccaaccaccg gctttgctgg cgttttcgtt atagatttcc
    39661 gcctcgcgat tgataaatac attagccagt acaccatagg ctttacccca cgcgtccagc
    39721 acttcctgcc ccgggctgaa catttcgtcc agcgttgcca acaggtgttc accgacgatg
    39781 ttgtactgtt ccggtttgat ctggaagctg gtgtgcttct gcgcgatttt ttctaccgct
    39841 ggcagcagcg caggcaggtt ttcaatatta ctggcgtagg cggcaatagc gttaaacagg
    39901 gcttcacgtt gatcgccatt acgctggtta ctcatgttaa aaatttcttt gagttctggg
    39961 ttatgagtaa acatacggtc gtagaaatgg gcggttaact ttggccccgt ttccaccagt
    40021 aaagggatgg tggcttttac tgtagcgatg gtttgagcgt caagcatatg gtcttccttt
    40081 tttgcatctt aattgatgta tctcaaatgc atcttataaa aaatagccct gcaatgtaaa
    40141 tggttctttg gtgtttttca gaaagaatgt gatgaagtga aaaatttgca tcacaaacct
    40201 gaaaagaaat ccgtttccgg ttgcaagctc tttattctcc aaagccttgc gtagcctgaa
    40261 ggtaatcgtt tgcgtaaatt cctttgtcaa gacctgttat cgcacaatga ttcggttata
    40321 ctgttcgccg ttgtccaaca ggaccgccta taaaggccaa aaattttatt gttagctgag
    40381 tcaggagatg cggatgttaa agcgtgaaat gaacattgcc gattatgatg ccgaactgtg
    40441 gcaggctatg gagcaggaaa aagtacgtca ggaagagcac atcgaactga tcgcctccga
    40501 aaactacacc agcccgcgcg taatgcaggc gcagggttct cagctgacca acaaatatgc
    40561 tgaaggttat ccgggcaaac gctactacgg cggttgcgag tatgttgata tcgttgaaca
    40621 actggcgatc gatcgtgcga aagaactgtt cggcgctgac tacgctaacg tccagccgca
    40681 ctccggctcc caggctaact ttgcggtcta caccgcgctg ctggaaccag gtgataccgt
    40741 tctgggtatg aacttggcgc atggcggtca cctgactcac ggttctccgg ttaacttctc
    40801 cggtaaactg tacaacatcg ttccttacgg tatcgatgct accggtcata tcgattacgc
    40861 cgatctggaa aaacaagcca aagaacacaa gccgaaaatg attatcggtg gtttctctgc
    40921 atattccggc gtggttgact gggcgaaaat gcgtgaaatc gctgacagca tcggtgctta
    40981 cctgttcgtt gatatggcgc acgttgcggg cctggttgct gctggcgtct acccgaaccc
    41041 ggttcctcat gctcacgttg ttactaccac cactcacaaa accctggcgg gtccgcgcgg
    41101 cggcctgatc ctggcgaaag gtggtagcga agagctgtac aaaaaactga actctgccgt
    41161 tttccctggt ggtcagggcg gtccgttgat gcacgtaatc gccggtaaag cggttgctct
    41221 gaaagaagcg atggagcctg agttcaaaac ttaccagcag caggtcgcga aaaacgctaa
    41281 agcgatggta gaagtgttcc tcgagcgcgg ctacaaagtg gtttccggcg gcactgataa
    41341 ccacctgttc ctggttgatc tggttgataa aaacctgacc ggtaaagaag cagacgccgc
    41401 tctgggccgt gctaacatca ccgtcaacaa aaacagcgta ccgaacgatc cgaagagccc
    41461 gtttgtgacc tccggtattc gtgtaggtac tccggcgatt acccgtcgcg gctttaaaga
    41521 agccgaagcg aaagaactgg ctggctggat gtgtgacgtg ctggacagca tcaatgatga
    41581 agccgttatc gagcgcatca aaggtaaagt tctcgacatc tgcgcacgtt acccggttta
    41641 cgcataagcg aaacggtgat ttgctgacaa tgtgctcgtt gttcatgttg gatgcggcat
    41701 gaacaccttc ttaggcctac aaaatcttgc aaattcaata tattgcaatc tccgtgtagg
    41761 cctgataagc gtagcgcatc aggcaatttt tcgtttatga tcatcaaggc ttccttcggg
    41821 aagcctttct acgttatcgc gccatcaaat ctgtcgtaac tgcgcctcaa catacaaata
    41881 gccaattccc agcacctgtt gtgcgcggct taattgcccg aagccaattt gcgtcgcttt
    41941 cacgctcggt ccttcgtcgc ggtcgaacgg gttaaatcct gtctggcgaa taatagtatt
    42001 aagccagttc tcaccaaagg cacaactgcg accgtacaac caaatctgat taatattgag
    42061 gatgttgagg aagttataca gactcaaacc aatggcattg gcagagcgat caacccagct
    42121 ggtgatccac agttctccac tctgccaggc agcgattaac tgcgcggttg tcagtttttc
    42181 aggatcaagc tgagtattaa ccggttgtga ttttagccat acccgcgcct gtttttttaa
    42241 tgcacttaac gaggcgacgg tttccaggca gccgtaacgt ccacagtcgc agacgacgcc
    42301 atccggatta acgatggtgt gaccaatctg tccgctacca tacaaactgc cgcgataaat
    42361 ttgctcgtta atcacgaacg acgagccaat gccgtaatca acgttgatca cgcagaaatc
    42421 ccgttcctgc gaattatttt gccatttctc cgccagcgcc agcatcacgc agtcattatc
    42481 gaccagcacc cgaatgccca gcttctcttc cagcagatac ttcacctcaa ctggcgttgt
    42541 ccacggcgct tgcggcatgg tttgcgatac gccggtcacc ggatcaacct gcccgtggat
    42601 tgccagcgcc agattgatgg tgcggtccgg ccacaattta cggtggcgat gccagcattt
    42661 ttcgatttcg gacagcagcg cctgcggcgt cggtacatca atctgtaaat actcaaattc
    42721 gcctttcgga cttaaacaag cattagcaac ctgacactca atactggtgg gcgtcacgtt
    42781 caggcacagc gtccagtcac cttccggcgc aatcagccat gtaccgctac tatgcccgcg
    42841 cgtctggctt tcatcgtcaa tattcaccac ccgtttttcg ctttccagtt cctgcaaaat
    42901 attactgact gccggaatcg aaatttgcgc cagccgggcc agcgttgatt tattggcgcg
    42961 cttttgccgg tacagcagtt ccagaatcac gcatttgtta tggtggcgaa tctgttgatt
    43021 gttaatgcag gctctcatct tcactaaacc ttgtttagtt aattgcgtga ttattccgcg
    43081 aaaacattgc cagattgttc aatacactgc cacaaatctt ttaattcagt atgtcttgtt
    43141 aatattgagg gcaccatgac tccagtaaaa gtgtggcaag agcgcgttga gatcccgacc
    43201 tatgaaaccg ggccgcagga tatacatccc atgttcctgg aaaatcgcgt ttatcaggga
    43261 tcgtccggcg cggtttatcc ctacggcgtg accgatacgc tgagcgagca gaaaaccctg
    43321 aaatcctagc aggcggtgtg gctggaaaac gactacatca aagtgatgat cctgccggaa
    43381 ctgggcggtc gggtgcatcg cgcatgggat aaagtgaaac agcgcgattt tgtctatcac
    43441 aatgaagtca ttaaacctgc gctggtgggg ctgctgggac cgtggatctc cggcgggatt
    43501 gagtttaact ggccgcaaca ccatcgcccg accaccttta tgcccgttga tttcaccctc
    43561 gaagcccatg aagacggtgc acagacggtg tgggtcggcg aaacggagcc gatgcatggt
    43621 ttacaggtga tgacaggttt caccctgcgc cctgaccggg cggcactgga aatcgccagc
    43681 cgcgtctata acggcaacgc cacgccgcgt catttcttgt ggtgggccaa cccggcagtg
    43741 aaagggggtg aagggcatca gagcgttttc ccaccggatg tgactgcggt gtttgatcac
    43801 ggcaaacggg ccgtctccgc tttccccatc gccacttact acaaagtgga ctactccgcc
    43861 ggagtggaca tttctcgcta taaaaatgtg cctgttccaa cctcatatat ggccgaaaaa
    43921 tcgcagtacg attttgtcgg cgcgtggtgt cacgatgaag atggcggttt gctgcacgtt
    43981 gccaaccacc atattgcgcc aggtaaaaaa cagtggagtt ggggacacag tgaatttggc
    44041 caggcgtggg ataagagtct gaccgacaat aacggcccgt atatcgaact gatgaccggt
    44101 atttttgccg ataaccagcc tgattttacc tggcttgatg cttacgaaga gaagcgtttc
    44161 gagcagtatt tcctgcctta tcattctctg ggcatggtgc aaaatgcctc ccgcgatgcg
    44221 gtgataaaac tccagcgtag tgagcggggg attgagtggg ggctgtatgc catctctccg
    44281 ttgaacggat accgtctggc gatccgcgaa atcggcaaat gcaacgcgtt gctcgatgat
    44341 gccgtggccc tgacaccagc gaccgccatc cagggcgtgt tgcacggtat caatcctgac
    44401 agactgacca ttgaactctc cgatgccgac ggcaatattg tactgagtta tcaggaacat
    44461 cagccgcagg agttgccgtt gccggacgtc gccaaagcgc cactggcagc acaagacatt
    44521 accagtacag atgaagcctg gtttatcggt cagcatctgg agcaatatca tcacgccagc
    44581 cgttcaccgt tcgattacta cctgcgcggc gtggcgctgg acccgctgga ttatcgctgt
    44641 aacctggcgc tggcgatgct ggaatataac cgcgcagatt tcccgcaagc ggtggcgtat
    44701 gccagtcagg cgctgaaacg cgcacatgcg ctgaacaaaa acccgcagtg tggacaagca
    44761 agtttgattc gcgccagtgc ttacgaacgt cagggacaat atcaacaagc cgaagaggat
    44821 ttctggcgtg cggtctggag cggcaatagt aaagcaggcg gctattatgg tctggcacga
    44881 ctggcggcgc gtaatgataa cttcgacgcg ggtctggatt tttgccaaca aagtcttcgt
    44941 gcctgcccaa ccaatcagga agtgctttgc ttgcataatc tgctgctggt gttaagtggt
    45001 cgtcaggaca acgcgcgttt gcagcgcgag aaactgctgc gtgattatcc gctgaacgcc
    45061 actctgtggt ggctgaactg gttcgatggt cgtagcgaat cagccctcgc gcagtggcgc
    45121 ggtctgtgtc agggacgcga cgttaacgct ctgatgaccg ccgggcaact gattaactgg
    45181 ggaatgccca ccctggcggc agagatgctg aacgcactgg actgccagcg cacgctgccg
    45241 ctttacctgc aagccagctt gctgccgaaa gccgaacgtg gcgaactggt cgcaaaagcc
    45301 attgatgtct tcccgcagtt tgtccgtttc ccgaatacgc tggaagaagt ggcggcgctg
    45361 gagagtattg aagagtgctg gtttgctcgc catttactgg cctgcttcta ctacaacaaa
    45421 cgtagctaca acaaagccat tgccttatgg caacgttgcg tagagatgtc gccggagttt
    45481 gccgacggct ggcgcgggtt agcgatccat gcgtggaata agaaacacga ttatgagctg
    45541 gccgcgcgtt atcttgataa tgcttatcag cttgcgccgc aggatgcacg tctgcttttc
    45601 gaacgagatt tgctggataa gttaagtgga gccacaccgg agaaacgact ggcgcgtctg
    45661 gaaaataatc tggaaattgc gctgaaacgc gacgacatga ccgcagaact gctcaatttg
    45721 tggcatctca cgggtcaggc agacaaagcg gcggacattc tcgccacgcg taaattccac
    45781 ccgtgggaag gcggggaagg gaaggtcacc agtcagttta tcctcaacca gttattacgc
    45841 gcctggcagc atcttgatgc cagagagccg cagcaggcca gcgaactgct tcatgccgcg
    45901 ctgcattatc cggagaattt aagcgaaggc cgtttaccgg ggcaaactga taacgacatc
    45961 tggttctggc aggcgatatg cgccaacgcg cagggcgatg aaactgaagc gatgcgttgt
    46021 ttacgtctgg cggcgaccgg cgatcgcacc attaacatcc acagttatta caacgatcag
    46081 ccggttgatt atctcttctg gcaaggaatg gcgctgcgac tgctgggtga acagcaaacc
    46141 gcacagcaac tgtttagtga aatgaaacag tgggcgcaag agatggcgaa aaccagtatt
    46201 gaggcggatt tctttgctgt ttcacaacct gacctgttat cgctgtatgg cgatttacaa
    46261 cagcagcata aagaaaaatg cctgatggtg gcgatgctgg cgtccgcggg actcggggag
    46321 gttgcgcaat atgagtctgc tcgcgctgaa ttgacggcga ttaatccggc ctggccgaaa
    46381 gcggcattat tcaccactgt gatgcctttt atttttaact acgttcacta aaccctatta
    46441 actgtaatat ttgagcggca cgcgctgccg ctcatcacaa aataattaga ataagccgtt
    46501 gttaataaaa accctacagg aggcactatg cctaaaaaaa tgagaacaac ccgtaatcta
    46561 ttattaatgg ctacgctatt aggtagcgca ctatttgcca gggctgcgga taaagaaatg
    46621 accattggtg caatatacct tgatacccag ggatattacg ctggagtgcg tcagggcgtt
    46681 caggatgcgg caaaagattc ttcagtacag gtacagttaa ttgaaactaa cgcccagggt
    46741 gatatttcga aagaaagtac ctttgttgat accctcgtgg cgcgtaatgt cgatgccatt
    46801 attttatcgg cagtgtctga aaatggcagt agccgtaccg ttcgtcgcgc cagtgaagcg
    46861 ggtattccgg tgatttgcta caacacctgt attaatcaaa agggtgtcga taaatatgtc
    46921 tcggcgtatc tggtcggcga tccactggaa tttggtaaaa aactgggtaa cgctgccgcc
    46981 gattatttta ttgccaataa aattgaccag ccgaaaattg ccgtcatcaa ttgcga
//
LOCUS       DADLSE010000016        46015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLSE010000016 DADLSE010000000
VERSION     DADLSE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.016"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            181..647
                     /locus_tag="JN692_000904"
                     /pseudo
     CDS             181..647
                     /locus_tag="JN692_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
     gene            complement(859..1023)
                     /locus_tag="JN692_000905"
     CDS             complement(859..1023)
                     /locus_tag="JN692_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263284.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1046..1747)
                     /locus_tag="JN692_000906"
     CDS             complement(1046..1747)
                     /locus_tag="JN692_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7263285.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1852..2748
                     /locus_tag="JN692_000907"
     CDS             1852..2748
                     /locus_tag="JN692_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7263286.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            complement(2955..3323)
                     /locus_tag="JN692_000908"
     CDS             complement(2955..3323)
                     /locus_tag="JN692_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7263287.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(3616..4602)
                     /locus_tag="JN692_000909"
     CDS             complement(3616..4602)
                     /locus_tag="JN692_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7263288.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(4672..5154)
                     /locus_tag="JN692_000910"
     CDS             complement(4672..5154)
                     /locus_tag="JN692_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7263289.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            complement(5250..5549)
                     /locus_tag="JN692_000911"
     CDS             complement(5250..5549)
                     /locus_tag="JN692_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263290.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(5539..5943)
                     /locus_tag="JN692_000912"
     CDS             complement(5539..5943)
                     /locus_tag="JN692_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263291.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(5946..6251)
                     /locus_tag="JN692_000913"
     CDS             complement(5946..6251)
                     /locus_tag="JN692_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7263292.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(6289..6657)
                     /locus_tag="JN692_000914"
     CDS             complement(6289..6657)
                     /locus_tag="JN692_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HAY7263293.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN692_000915"
     CDS             complement(6804..7187)
                     /gene="mzrA"
                     /locus_tag="JN692_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7263294.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(7191..7853)
                     /locus_tag="JN692_000916"
     CDS             complement(7191..7853)
                     /locus_tag="JN692_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7263295.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEEQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN692_000917"
     CDS             complement(8198..8974)
                     /gene="exuR"
                     /locus_tag="JN692_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7263296.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            9270..9986
                     /locus_tag="JN692_000918"
     CDS             9270..9986
                     /locus_tag="JN692_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7263297.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAENQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            10016..10516
                     /locus_tag="JN692_000919"
     CDS             10016..10516
                     /locus_tag="JN692_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7263298.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            10608..11414
                     /locus_tag="JN692_000920"
     CDS             10608..11414
                     /locus_tag="JN692_000920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-fimbriae usher protein"
                     /protein_id="HAY7263299.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLN"
     gene            11442..11642
                     /locus_tag="JN692_000921"
     CDS             11442..11642
                     /locus_tag="JN692_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263300.1"
                     /translation="MVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVA
                     LKVYENNQLTRACAVYQNRRSD"
     gene            11611..13287
                     /locus_tag="JN692_000922"
     CDS             11611..13287
                     /locus_tag="JN692_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane fimbrial user protein"
                     /protein_id="HAY7263301.1"
                     /translation="MPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLH
                     PQYELYAGLANADDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSN
                     PGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTRTQNN
                     TDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNG
                     GDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSF
                     SHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSST
                     LAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDS
                     VLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNY
                     IGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVV
                     KKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            13740..14372
                     /locus_tag="JN692_000923"
     CDS             13740..14372
                     /locus_tag="JN692_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7263302.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(14460..15758)
                     /locus_tag="JN692_000924"
     CDS             complement(14460..15758)
                     /locus_tag="JN692_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263303.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            16241..17653
                     /gene="uxaC"
                     /locus_tag="JN692_000925"
     CDS             16241..17653
                     /gene="uxaC"
                     /locus_tag="JN692_000925"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7263304.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRCPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            17668..19155
                     /locus_tag="JN692_000926"
     CDS             17668..19155
                     /locus_tag="JN692_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7263305.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKRELGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            19238..19789
                     /locus_tag="JN692_000927"
     CDS             19238..19789
                     /locus_tag="JN692_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7263306.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN692_000928"
     CDS             complement(19794..21038)
                     /gene="sstT"
                     /locus_tag="JN692_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7263307.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN692_000929"
     CDS             complement(21634..22599)
                     /gene="alx"
                     /locus_tag="JN692_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7263308.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(22870..23886)
                     /locus_tag="JN692_000930"
     CDS             complement(22870..23886)
                     /locus_tag="JN692_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7263309.1"
                     /translation="MEYPRVMIRFAVIGTNWITRQFVEAAHERGKYKLTAVYSRSLEQ
                     AQHFANDFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPL
                     ASNLAEVDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSS
                     RYQRYLDGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAH
                     GVVVMDYGDFSVTLQHSKVSDSVLGSEIQGEAGALVIEKLSECQKVCFVPRGSQMQDL
                     TQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL
                     YLPK"
     gene            complement(23947..24639)
                     /locus_tag="JN692_000931"
     CDS             complement(23947..24639)
                     /locus_tag="JN692_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7263310.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGHKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(24716..25219)
                     /locus_tag="JN692_000932"
     CDS             complement(24716..25219)
                     /locus_tag="JN692_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7263311.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            25304..26440
                     /gene="rlmG"
                     /locus_tag="JN692_000933"
     CDS             25304..26440
                     /gene="rlmG"
                     /locus_tag="JN692_000933"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7263312.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKVVKLGRRR"
     gene            26725..27039
                     /locus_tag="JN692_000934"
     CDS             26725..27039
                     /locus_tag="JN692_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HAY7263313.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKE
                     KK"
     gene            27036..27452
                     /gene="higA"
                     /locus_tag="JN692_000935"
     CDS             27036..27452
                     /gene="higA"
                     /locus_tag="JN692_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7263314.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(27497..29515)
                     /locus_tag="JN692_000936"
     CDS             complement(27497..29515)
                     /locus_tag="JN692_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7263315.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKITLQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN692_000937"
                     /pseudo
     CDS             complement(29741..32092)
                     /gene="ygjK"
                     /locus_tag="JN692_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN692_000938"
                     /pseudo
     CDS             complement(32109..33180)
                     /gene="ygjJ"
                     /locus_tag="JN692_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(33314..34747)
                     /locus_tag="JN692_000939"
     CDS             complement(33314..34747)
                     /locus_tag="JN692_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7263316.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(34810..35259)
                     /locus_tag="JN692_000940"
     CDS             complement(34810..35259)
                     /locus_tag="JN692_000940"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7263317.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN692_000941"
     CDS             complement(35256..38348)
                     /gene="ebgA"
                     /locus_tag="JN692_000941"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7263318.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSH
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN692_000942"
     CDS             complement(38532..39515)
                     /gene="ebgR"
                     /locus_tag="JN692_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7263319.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            39734..40065
                     /locus_tag="JN692_000943"
                     /pseudo
     CDS             39734..40065
                     /locus_tag="JN692_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN692_000944"
     CDS             complement(40107..41597)
                     /gene="ygjG"
                     /locus_tag="JN692_000944"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7263320.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            41904..43424
                     /locus_tag="JN692_000945"
     CDS             41904..43424
                     /locus_tag="JN692_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7263321.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAILWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVAIMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQFGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(43578..44201)
                     /locus_tag="JN692_000946"
     CDS             complement(43578..44201)
                     /locus_tag="JN692_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7263322.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            44478..45242
                     /gene="nfeF"
                     /locus_tag="JN692_000947"
     CDS             44478..45242
                     /gene="nfeF"
                     /locus_tag="JN692_000947"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HAY7263323.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEKYDPQRVRAAAYWHAK"
     gene            complement(45296..45371)
                     /locus_tag="JN692_000948"
     tRNA            complement(45296..45371)
                     /locus_tag="JN692_000948"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45336..45338),aa:Ile,seq:cat)
     gene            45496..46002
                     /gene="mug"
                     /locus_tag="JN692_000949"
     CDS             45496..46002
                     /gene="mug"
                     /locus_tag="JN692_000949"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7263324.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT        11372 a        11626 c        11930 g        11087 t
ORIGIN      
        1 aagtgggggc tgcatattgg cgcgacgctg gaaaaacagt gcgagcgcgg tttgctggcg
       61 aaagatctct cttcatccat tgtgattcgt accagcgcgg catccgatgc gcgtatgggc
      121 ggcgctacgc ttccggctat gagtaactcc ggctcgggta accaggggat caccgcaaca
      181 atgcctgtgg tggttgtagc agaacacttc ggagcggatg atgaacgact ggcgcgtgcg
      241 ctgatgcttt ctcatttgag cgcaatttac atccataacc agttaccgcg tttgtctgca
      301 ctttgtgccg caacgaccgc agcaatgggg ccgccgccgg gatggcatgg ctggtggatg
      361 ggcgttatga aactatctcg atggcgatca gcagtatgat cggcgatgtc agcggcatga
      421 tttgcgatgg tgcgtcgaac agctgcgcga tgaaggtttc gaccagtgct tcggctgcgt
      481 ggaaagcggt gttaatggcg ctggatgata ccgccgtgac cggcaatgaa gggatcgtgg
      541 cgcatgatgt tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat tcgatgcagc
      601 aaacggatcg gcagattatc gagattatgg cgagcaaggc cagataaacg ccaggttttg
      661 agtattgtgc cggatgcgat gctggcgcac cttatccggc ctacggggtg ctcaacttgt
      721 tgatgttgtt gtgttttgta cctgatgccg gatgcgatgc tggcgcatct tatccggcct
      781 acggggtgtt taatttgttg atgttgttgt gttttgtagg ccggataagg cgtttacgcc
      841 gcatccggca acctgtcttt acgcggtagc ttcatcctcg gctaaacgcg tttcagcatc
      901 cgcgacgata gcttccagct cgctcaacat ctcttcatag ccaaaatttt tggcggtgcg
      961 agcaggttct ttcgccacca cgggcttcgc tggctggcgc tttttggtcg attttttact
     1021 catcatcatc tccctgtaaa aacaactaga caggcaaatc tatcagcaaa gcgcgcagtg
     1081 gggaatcggc aaccagcgta atgttagcct cgtcacgaat aaacgcccca tcaccgcagg
     1141 ttagcgccgc tttctcttca tgatgcgtaa gcgcatgaaa tttcccgtga atcgattgca
     1201 aatacgcgcg tggcccatgc aactggaaat tcgcactttc gcctttgtcg agcacgatat
     1261 ggtgcagcca cacttgttgg cgtaattgca ggcttcccat cgcaccctct ggcgaggcga
     1321 ttaattgctg cttgtccata ttaagcgcca gcttttgaat cagcggattc tctcgctgcg
     1381 ggcaggtatc cagccaaagc tgcattcgcg ttaacggttt gtctttgctg agattgtgtt
     1441 cgctatagct gacacccggc tgggtagaga gcagcaacgc ctctccggcg ctggcctgaa
     1501 catgattgcc ttcgctgtcg cgatactctg cttctccatc cagaatcaca tttaaaatat
     1561 cgactttggg ataggttcgc ggctgaaagg cggcacctgg ggccagcact tcctggttaa
     1621 gcacacgcag ggaggcatag cctaacaatt tcgggtcgaa gtagtgtcca aaggaaaaag
     1681 tataccgggc ctgcaaccat ccgtagtctg cttgtccaca ctgcctggca gttcgggtag
     1741 taatcataat tcttgacctc tctttacttc cttttatggt aaggggctgg acgctacatt
     1801 gttagccaga tattctgccc ggtatgttca aatttcctga atgagaacga aatggccaaa
     1861 gaaagggcat taacgctgga agcactacgg gttatggatg cgatcgatcg ccggggcagt
     1921 tttgcggcgg cagcggatga gctgggacgc gtgccttccg cacttagcta caccatgcaa
     1981 aaactggaag aagagctgga tgtggtgctg tttgaccgct cgggccatcg caccaaattc
     2041 accaatgtcg ggaggatgtt gctggagcga gggcgcgttt tgctggaagc cgcagataaa
     2101 ctgactactg atgcggaagc tctcgcgcgc ggttgggaaa cgcatctcac cattgtgacc
     2161 gaagcgctgg taccgacacc tgcctttttc ccgttaatcg acaaactggc ggcaaaagcc
     2221 aatacccaac tggcaatcat cacagaagtg ctggcggggg cgtgggaacg gctggagcag
     2281 gggcgggcgg atatcgttat cgcgccggat atgcattttc gttcctcgtc ggagatcaac
     2341 tcgcgcaagc tctatacgtt aatgaacgtc tacgttgccg cgcctgatca cccgattcat
     2401 caggagccgg aaccgttatc tgaagtgacg cgtgtgaaat atcgtggaat tgcggtagcg
     2461 gataccgctc gtgagcgccc ggtgttgacc gtacagctgc tggacaaaca gccgcgctta
     2521 acggtgagca cgattgaaga taaacgtcag gcattactgg cggggcttgg cgtggcgacg
     2581 atgccgtatc cgatggtcga aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg
     2641 gaatcgacca gcgagatcga tattattatg gcctggcgtc gtgacagtat gggggaagcg
     2701 aaatcctggt gtctgcggga aattccaaag ttgttcgctg gaaaataatc tattaagcac
     2761 ctttcacaag gtgcttctga ctgctgacaa acgcaaaact gcctgatgcg ctacgcttat
     2821 caggcctacg cagtcactgc aatatattga attttggcaa tttgtaggct ggataaggcg
     2881 tttacgccgc atccggcata aacaaagcgc actttgtcaa caatttgaag caccttttac
     2941 aaggtgctta atcattattc ttgttccagt tttggatctg gtccaaagcg gttttcgcct
     3001 ggcgtacccg cctggcagtt aaagacgatg ataataagcc agccgataaa cgggattaag
     3061 aatagcagtg cccaccacgc cgagcggtcg gtgtcgtgca ggcggcggaa ctgaaccgcc
     3121 caccacggta aaaacaccag aataccgtag attgtcgtca ggatgccttc gccaccagcg
     3181 cgttgccagc ctaacatttt atccagcagc cccagcacga acgtaaagat gatgttgacc
     3241 agaataaaca tccagtactc tttgcgccgc gcacgacccc ggaaaccgac ataatttttt
     3301 agtactttca gataccagtc catttttgcc tcaattttgc tcaatcactt gtttttaaag
     3361 cagcaaatta agaatagacg tgaatgaaat ggcagtaaat attcgttcaa ggaattaata
     3421 aagtgccgtg aaggcgcgct aagactgatg gcaagcgcaa aatcgccaga tgcgctacgc
     3481 ttatctggcc tacgcggtca ttgtaacata ttgaatataa acgctttgta ggtttgataa
     3541 ggcgttcacg ccgcatccga catgaacaac atgcacttag ctaattatca gaggcacctc
     3601 gtaaggtgcc tttttttaac cgaagcgaac atctcgtcca tgcggttcat cgagatcctg
     3661 ccacggacca attgaaataa tccccgtagg gttgatggtc ttatggctgc ggaagtaatg
     3721 attacggatg tgatcgaaat tgacagtttc ggcgatcccc ggcatctggt agatatcgcg
     3781 caggaagcca tacagattca ggtaatcgct gatgcggtgc ttatcacact tgaagtgggt
     3841 cacatacact ggatcaaaac gcaccagcgt agtccacagg cgaatatcgg cttcggttag
     3901 ctggttgccg gtcaggtaac gatgctggcc taaaatctgc tccagtcgcg ccagcgattc
     3961 aaacactttc gccaccgcct cgtcgtaggc ttgctggctg gtggcaaaac cagctttata
     4021 cacgccgttg ttaacggtgt cataaatcca gccgttaagt tcgtcaattt tcgtttgcag
     4081 ggctggtggg tagtaatcac ccgcttttgc gcccagcgca tcaaacgcgg tattgaacat
     4141 gcggatgatt tctgctgatt cgttgctgac gatggtgtgg ttctttttgt cccacagcac
     4201 gggaacagta actcgtccgc tgtagtgtgg atcggcgtgg agataaagct gatacagaaa
     4261 ttcatgttga tagagcgtat ctccggttgc tcccggaaaa ctgtcatcaa aggtccagcc
     4321 gttttccagc atcagcgggt tcactacgga aacggaaata aacggttcca gtcctttgag
     4381 tttgcgcatg atcagcgtgc ggtgcgccca cgggcaggcg agtgaaacat agagatgata
     4441 acgatctttc tctgcgataa aaccgcctgt gccagtgggg ccaggtgcgc catcggcagt
     4501 gagccagtta cgaaatgcgg aagctgaacg ttgaaattta ccgccggtag atttggtatc
     4561 gtaccaggtg tcatgccaga cgccgtcaat cagttgaccc atttttctct cctccaatag
     4621 caaaaacgag gagcttcctc ctcgtttttt atcgttaagt atagcgcgtg cttaccactt
     4681 tttattcagc aggcggtcga tgctatacgc gcccggaccg gtaattgcca gcagcaggaa
     4741 tccgccagaa attgtcaggt ttttcatgaa catcagcgag ttgacgcctt cagcaaagtt
     4801 gctgtgaaat aaaaatgccg tcagcagcgt aaagcccgca gtaaacaggg ctgtggtgcg
     4861 agtcaggaaa ccgaacagga ttgccagacc accaccgaac tcaagcagaa tcaccagcgg
     4921 cagcataaaa cccgggacac ccattgcttc catatattgt tgggtaccag agtaagcggt
     4981 gatttttccc cagcctgcgg taataaacag aatcggcatt aaaatgtgcg ctaccagtac
     5041 accaacatct tctaattttt tcatcatact ctccactgcg ccactcaggc tgctgattgt
     5101 ttttttgtgc aagggcgcgg tattagcgtc gttgctgtcg atggagagaa tcataaacgt
     5161 ggtgaatgat gattgttagc aaggaaaact gtcaaaaata ttcaaaaaat ttgagggata
     5221 aggccggaat tgctccggcc agagggaagt taaccgcgaa gctgttgctg cttgagggtc
     5281 gttttaacca gacgccaggc gctccatacg ccaaaaccgc gtctggccca gcggaccagc
     5341 atattaggat ggcgaatcgt ccagatcgcc atcacgctac tgccaaccag cgcccaggag
     5401 cgcagactta gcagcatatt ccagcgacga tcgtaagcgc ctgttgcctc cagccattca
     5461 cgacgactgg cggaaagatc cagccgttgt tgctggatct ggctaagcag ttgcgcctta
     5521 cgtcgttcac gttcgacttt actgctcacg ggactcctcc tcgagcagtt gccgatcgtt
     5581 tgccagctca tggcgtgtat ggcgcagcaa ggtggattta cgcgatttac gtaacgtcca
     5641 gataccgcca atcagtgcca gtagcagcaa caccacggtg gtggcaatca tcgcattcag
     5701 gcgatattgc ggatcaaccg cccaaataat tagcaccatc aggctcataa gaccaaatgc
     5761 agcgaaaagc atcgtcaggc ccagcatcag taaaagttga aagagattag ctttttcctc
     5821 ttccagctcc accaccgcca gccgcagacg tgtttccacc atttcaacca tgatggaaac
     5881 aattcgctgc ccgatgccca gaacgctttt accgggccct tgcgcgtgat gagtgtccgc
     5941 cataattaac gacgcgacag cagaacgccg agcactacac cgattgcagc gccaattccc
     6001 acgccggtcc acggattttc acgcacatac tcatcagcac gcgccgccgc gacacgggtt
     6061 tgtttggcga tggcatcacc ggtttcaccc aggcgataac ggctctgttt cagtgcctgc
     6121 tccgctttgc tacgaatctt actcaactct tctttcgact tctcgccaga tgagctaagc
     6181 acctcttcca gcgtatcgga aagggatttc aactcagcac gcagatgttc cgtagtgtgt
     6241 tctttcgaca tagttttctc caggtgagta agtagtgact attgtgagtt agtagtcgcg
     6301 cgcttccagc tttttcagct cttcctgcgc ttctgccagt ttccgttcgc gtttggcaat
     6361 cttatcggca tcgccttttt gcttcgcctc ggctaaatct tgctggcgtt ccgccacctc
     6421 atctttctgc tttgcgattt tcttgtgatg atcggcacgc agctggctat ctgaacagtt
     6481 ggcccggact tcactcaggg ctttattcag accgtcaata cgattctggt tttggtgttt
     6541 ttcggcatag ctgatctcct taaggatatt ttgctccttt tcctgacaca gggtagtggc
     6601 ataactaccg gcactaagag caaagagaga aacagctaaa gcgatgcggt atttcattct
     6661 tgaaccttcc attgtgattc ggacctatta caaaggaacg attgtccaga caacgaagcg
     6721 gtagtgttaa ccaccgcctc attaatcata gacagttacg gtgaaatcac caaagtgagt
     6781 gatatgtttc ggataacagg aagttatccg aagcgatgag agttatcccg taaccgggtc
     6841 agccactgca tagcctgact gttattgtcc tgctgcgcaa tgatgtagcc atggggcaat
     6901 gttctgtcga ggaccgcttt tgcggcggcg ctctggtcgc tggagtcaaa tgtaattaac
     6961 agagtgtcgt ttttgggggt gatacttttg aaaggaatgc catgagcgtc aaggtgatgc
     7021 cagatcgaaa aaccgtctgg catcgttgtg ccttgatgaa cggcacgaat cgccagcgta
     7081 gactcttgct ggcgaaccgc ggaccaggcc agcaacagcg tgccgaccag aagtaaaaca
     7141 gtgccggacc aggctagctg gcgaagcgac atgcgaggta tttgcatccc ttacccccga
     7201 tttccatatt tctttttcca taacacgacc agagaacctg ctaggccaaa caccagcagc
     7261 accaccggga gcagcatcag gcatgacatc agctgttcct cgtactttaa aaataccggc
     7321 gttttgccga gcatgtaacc cagagttgtc aggatcaata cccacagcag accgctcatc
     7381 cagttgaaga actgaaagcg cgcgttattc agccctgata acccggcaat cgtcggcagc
     7441 agtgttctga caaatgcaat aaagcgacca attaacagcg ccgataaacc gtgtttatga
     7501 aaaagatggt gtgcgcgttg atgataatgc gcgggtaaat gagatagcca gttttgtacg
     7561 gtgcgggtat tgcccagcca tcgcccctga atatagctga cccagcaacc gaggctggcg
     7621 gcaacggtca gcagcagaat cgtttgcggg taacccatcg cgcctttcgc aatcaacacg
     7681 ccgaccaata ccagtaaact gtcgcccggt aaaaaggccg ccggaagcaa gccattttca
     7741 aggaacaaaa ttacaaacaa gacaaaatac aacatgccaa tcatcgatgg attggccagg
     7801 gtttcaaaat cctgcgccca cagggcttgc agcaattggg tcaaaagttc cattcattat
     7861 tcctggtgaa tcggttaacg ccacgctggt tttaaaagat gcactaccgc aatgttattg
     7921 ctgttttggt atgttctgcg ggtttgatac atcaacacag cctgaactat tccctgttaa
     7981 aacgtgagat aagaaagcat tcaaaagcat aaattcaaat tgaatctaat tgtaacaaag
     8041 gtcaacgttg ttcgttatca aaattacatg tccgtggagt ctgattttgc tgattgcagg
     8101 ggagggtggc gagaggattt acacaggctg acactttata cttttcgctt accgacgaga
     8161 caaacaggcg cattgcagcg cctgtcggac aggaatttca tttgctgccg ctggtggccg
     8221 tatcgagatg cactaccggg ttttcggcga acagatagcg gtcggcattg aactcgaaat
     8281 cgtcgctggt ttcgttaaat aacatgattt tggtgttttc cagatgctgc cacattgcca
     8341 gcttagcagc atgaggatct ttgcgaatta acgccttgag aatttgatcg tggtcatcac
     8401 accagttatc aacggtacgg gaatcaatgt gttcgtgtag ttttttccag tacgggttat
     8461 ggctacgctg ggtccacatt ttttcaacga tggccgccag ggcggagttc tgcgtcgcca
     8521 gggctacctg aatgtggaac tgtaaatccc actcggaatc acggaagcat tgttcgccgc
     8581 gcgcctgttc ctgaatggcc atcagtttca tgatgtcttg tttcgttacc tgagttgccg
     8641 cgaactcggc aatattactt tcgatgagct ggcgagcctg aagcaactca aacggaccgt
     8701 aattggcgaa ttccatatta ttgtcagccg cctgctgatg gcgcggctgg ttggaaacca
     8761 catgaatacc tgaaccttta cgcacctcca catagccttc aacttccagc atgatgatgg
     8821 cttcacgaac taccgttcgg ctgacgttct tttcatcggc aataaagcgt tcggcaggca
     8881 gtttatcacc caccagatag acgccctgtt caatgcgctc tttcaggtca gcagcaagtt
     8941 gttgatacaa acggcgtggt tcagtgattt ccatatgcgt ctcccggagt ggaatgcgga
     9001 cgaagttatc ataccacttt attcatgggt atgccattgg tcatatataa atttgatgat
     9061 tgaattaatg tattaaatta atattgtgaa tatttataaa ttcctaaaaa ataagtatcg
     9121 tcattgtgaa agctactaaa tttcagtaag ttaattccca gcctttaaat ttaatgttaa
     9181 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
     9241 ttgaaacgat attgtaagat caaaattata tggtggcgaa aattaatctc ttattacggc
     9301 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
     9361 ctatggctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
     9421 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
     9481 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
     9541 tcgctttacc agccggtagt agcaaaaccg tacgttttgt tgcgatggaa ccagagcaaa
     9601 aagagaaaaa ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag
     9661 ataaaaaaga tctctctatg cagttaacag ttaacttaat ttggggaatc gttgttagtg
     9721 ttccacctca gcaacctata gctaagttag aagtaaattc tgcccaaaaa ttaattaatg
     9781 cagagaatca acgcttaaag cttttaacga tcgcttattg taaaaataat agcaaagaaa
     9841 actgtaagat acagaccgta aataaaaaca tcttccctgg tcaggaaaga aatcttgaaa
     9901 gcatatctgg ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg
     9961 gcgagtttga actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa
    10021 aaaggtattt gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt
    10081 ggcagtacaa aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca
    10141 gaccgataac accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct
    10201 gcaatcttac cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat
    10261 gcagttagtg tccccagcgc aactggttca aagcctggat gctagcaaga ttgttccgct
    10321 gacagtaacc tggggtggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac
    10381 caaaatcttt gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc
    10441 tcaaaccacc aaaggtgttc tggaaacagg catctaccgt ggtgtagtaa gcatttatct
    10501 gtctcaggat atttaatccg gataacagaa ttaacacgta acaaagggag gggtagccct
    10561 cccttatttt aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat
    10621 tactggctct tttgatcctg gcgagtctca gcccggcaga ggcgacatta accaaaattc
    10681 ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag
    10741 ggaaaagtct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc
    10801 cagcaagtct atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag
    10861 tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac
    10921 gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg
    10981 aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt
    11041 cattatattt aaatccagac aaaaatactg ttaacgcttt tatacatcag caagacatca
    11101 atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa
    11161 taacagaaaa tagctatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg
    11221 tcagtgacag taatactgac gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt
    11281 attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact
    11341 ttacctttaa ctttctgcca ctcggtgcaa tcgatgggat gcgtatcggg tcgacactga
    11401 gctatttaaa ctaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca
    11461 attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat ctcaatagtg
    11521 gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg gcattaaaag
    11581 tctacgaaaa taaccaactc acccgagctt gtgccgttta ccaaaaccgg cggtctgact
    11641 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
    11701 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
    11761 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
    11821 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
    11881 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
    11941 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
    12001 ttaagctgtt atgaaagtat ttccgcgaca gtttcactga attttgtcgg ctggaatatg
    12061 atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg ggataaacag
    12121 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
    12181 caacttagcg cttcccgcgc tattgtgatg cgtgactgga ttttgagtac gtcagtcggt
    12241 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
    12301 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac caatgtgtct
    12361 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
    12421 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
    12481 gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa tgtctacgct
    12541 accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt tacgggcact
    12601 tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag tggtacagac
    12661 gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga tgaagagagt
    12721 caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt
    12781 gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt taacgacagt
    12841 agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa tcgtgaatta
    12901 atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa ctacaactat
    12961 atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc
    13021 gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg cagcgaaaaa
    13081 gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactgagtgt tgtaaagaaa
    13141 cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta ttcacaatta
    13201 ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg aaacgttcag
    13261 acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcggcgata ttgggcttgt
    13321 ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt acgtatgatt
    13381 tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca ggaaataatg
    13441 gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca gctaatggaa
    13501 atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt tggccgataa
    13561 aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcta ggatgcaagg
    13621 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
    13681 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
    13741 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
    13801 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
    13861 tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg catccaacag
    13921 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
    13981 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
    14041 ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc accaccgacg
    14101 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
    14161 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt gtgcgtccgg
    14221 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg actctggcag
    14281 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
    14341 tctttacgcc ttccttgcca accatcaatt aaccctgcaa accggacaaa cgcttttttc
    14401 tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta cgttaaacgt
    14461 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
    14521 tgcaatacgg tccagataac cagcgcaccc agcaggtcaa atactgccag aactgcgaac
    14581 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
    14641 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
    14701 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
    14761 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
    14821 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
    14881 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
    14941 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
    15001 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagt
    15061 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
    15121 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
    15181 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
    15241 aatgcaccgg agatgataaa tgccatctgc cagctgtgca tcacgattgc ccataccacc
    15301 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtagcc tactgcgatg
    15361 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
    15421 gcttccgcgg caccgaccgc accacgagca acagccaggc caccccagct acctgccagc
    15481 gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc gattttcgta
    15541 cccagcacat ccagtacata acctgctacc ggttgcatga ccgtataagc agcagaatag
    15601 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
    15661 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccgag cgtcaccagt
    15721 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
    15781 tatccgcagg aagcgcgacc atcatcccgc cagaggctga agatgttagt tacgaccggg
    15841 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
    15901 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
    15961 cgacttatga tttgcgacgg cagaaagata acttgtcata caaccttaaa aggtgagagc
    16021 catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
    16081 cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg tttattgatc
    16141 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
    16201 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
    16261 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
    16321 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
    16381 taaaaacctg tatgacatct ggttgaaggg cgatcactac aaatggcgcg ctatgcgtac
    16441 caacggtgtg gctgagcgtc tgtgtactgg tgatgcgtct gaccgtgaaa aatttgacgc
    16501 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
    16561 actgcgttgt ccgttcggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
    16621 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
    16681 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
    16741 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
    16801 caaagccttc aatatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
    16861 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
    16921 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
    16981 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaaaccct
    17041 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
    17101 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
    17161 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
    17221 ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctactgc cgaaaaccat
    17281 tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg gtaacttcca
    17341 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
    17401 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga gccgctttgt
    17461 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
    17521 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
    17581 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
    17641 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
    17701 gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga taaccagact
    17761 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
    17821 ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
    17881 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
    17941 tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt gcagatctat
    18001 cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc
    18061 tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac caacaacgcc
    18121 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca actgggcgac
    18181 gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa cgcgggcgca
    18241 gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg
    18301 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
    18361 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
    18421 ctaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
    18481 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
    18541 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
    18601 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt caaacagtac
    18661 tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa agcgggcggt
    18721 atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc cagtgtcgtg
    18781 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg
    18841 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
    18901 ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa aatcgccacc
    18961 aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg tcagctgatc
    19021 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
    19081 aacggtaagc aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
    19141 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
    19201 gcgccgtttg cttttccata cctgctaagg atttatcatg accgcgtatt ggctggccca
    19261 gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaacg
    19321 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
    19381 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
    19441 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
    19501 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtacctg
    19561 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
    19621 ctgggtgatt cacaacttct gggcggggtc gataggcggc acgatgattg agggtagttt
    19681 tctgcttatg aatggcctga atatcattcg tttctggcgg atgcagaaaa ggggaattga
    19741 tccgtttaaa gtagagaaaa cccctcccgc catagacgaa aggggttaaa caattaatta
    19801 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    19861 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gataataaag
    19921 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagt
    19981 gggatcagca gcaaagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
    20041 actacgctca acagcagcgc tgtgggcaga tcgaccggaa tacccagcgt attaaccgca
    20101 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggctcccagc
    20161 ggaatagaaa ctgaataggt atcgcgatcc agattcagct tttcacacag cgccatattc
    20221 accggaatgt tcgctgcaga gctgcgggtg aagaaggcat acacaccgct ttcgcgcagg
    20281 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    20341 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    20401 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagcccaaa aataccgatc
    20461 ggtgcgaagc gaatgaccag tttcaccata aaggtaacgg cattcgacat atcattgacc
    20521 aggtttttgg tggtctcgtt accgtgacgc agtgcgaagc cgaggccgat cgcccacacc
    20581 agaatcccga tgtagttacc tttcagcagg gcgtcgatgg ggttggaaac catgctcatt
    20641 accagtccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    20701 gacaagtgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agctgcagag
    20761 aaggtgccca gcagatagag gaacaaaata ggacggatat tggttttctg cccgtgctgg
    20821 tggttagcaa tagatgccat taccagcatc aacaccagga tgggggcaac agctttcagt
    20881 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    20941 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttactaggct gccatgagcc
    21001 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    21061 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    21121 aggtcgagta taaggaaagt tacagtttcg ggaagcgaaa aatgctggat tttaaagaga
    21181 ggtcatgttt tgcaacttaa tggttgcatt tatttaacat aaacagaggg aaggactggc
    21241 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
    21301 tcctttaacg tgttatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcggcca
    21361 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcggttcc
    21421 ctcagcctat cccttgcggc taccgggtcg ggcgcaaggt aacgtccctg taagatgcgc
    21481 cctcgccccg gcctccaggg aacgcttcgg cgattttgac gccaccaaca cccgtgcggt
    21541 ctttttgtaa agagctataa gccgattaca taattaatag ctcacctttg tctggaatga
    21601 gttatctcta ggaatggctt aatatacagc gaatcattct ccccgctgct tatcatgccg
    21661 ataattcacc caggcgttga taataaacgt catcaccaga atgccaaaca ccacgcccag
    21721 cgagacggcg attggaatat ggtagaagtc gacaatcagc atcttgatac cgataaacac
    21781 cagaatcacc gccaggccat atttgagcat cgagaaacgc tctgctacgc ccgccagcag
    21841 gaaatacatc gcacgcaggc cgaggattgc aaacaggttt gaggtcagca caatgaacgg
    21901 gtcagtggtc acggcgaaga tagccggaat gctatccacg gcaaaaatca cgtcgctcaa
    21961 ttccaccaga atcagtacca gcatcagcgg tgtggcgtac agcaatccat tcttacgcac
    22021 aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa tgaccgcgta gccagcgcac
    22081 cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg agcgccatct tcacgccggt
    22141 aaacagcagg aaggcaccga agatatacag gatccagtcg aactgcgaaa tcagccagct
    22201 gcctgtgaag atcatgatgg tacgcagaac aatcgccccg agcacgccat acaccagcac
    22261 gcgacgttgt aatgccgccg gaacggagaa atagctgaac aacatcagcc agacaaagac
    22321 gttatcgacc gccagcgatt tctcaatcag ataacccgtg agaaaggcca gggcctgtgg
    22381 atcggcaacg gcgcgacctt cggtttgcac cagataccac cagaaagcgg cgttaaacag
    22441 taacgacagc gtcacccaga ccagcgacca ggccgcagcc tgtttcatgg tcatggcatg
    22501 tgccccacga cgcccctgca acaacaggtc gatagccagc ataatggcga caacaacagc
    22561 gaatccgccc catagcaacg gcgtgccgac agtattcata gaagttcctt acacataaaa
    22621 aaacggccaa cgtcggaaga cgtcagccgc tgctttttat gcatagacct cgccttccgg
    22681 caaggtctca cttacaacaa aaaaggaata tgtctgcgta ttcctttcgg gttgcccggt
    22741 gaccggatgt ggtttttcac acatcgtaat gacgatcgac cggcaatgaa gttactcccc
    22801 tttgcaggta acaaagtatg acagaccatt cagtcagtca atggtctgtt acataccttt
    22861 tacactgttt tactttggca agtatagttt tacgctatct gccggaaaaa tcaccccagt
    22921 ctggcggcgg atctcggtca gcagtttggc tgtgatgcga ctgaccgcca gccctggatg
    22981 atccaccaga tgctcatcca ccagggtagc gaacagctct gcttcgtaga gcatggtatt
    23041 aatatgctgc ggctgggtga gatcctgcat ctggctgcca cgcggcacaa agcagacttt
    23101 ctggcattca gacagttttt caatcaccag cgctcccgct tcgccctgaa tctcgcttcc
    23161 caggacagaa tcactgacct ttgagtgctg caaggtgacg ctgaaatcac cgtaatccat
    23221 caccaccacg ccgtgggcgt caacgccgct tgccagcaaa ctcgccgttg cctgcacgct
    23281 tttcggctca ccaaataacg ccaccgccga cgccaggcag taaaagccga tatccataat
    23341 tgaaccgtta gagaatgccg gattaaaggt gttgggattc tcaccgtcca ggtaacgttg
    23401 ataccgcgag gaatactggc aatagttgaa aaagacttta cgcagtttgc ctgctttcgg
    23461 cagcgcctgg cgcaacaaat gaaagttcgg caggcaggcg gttttaaatg cctcaaacag
    23521 caccacctga ttttcgcgcg cacaggcaat ggcggcatcc acttccgcca gattcgacgc
    23581 cagcggtttt tcgcaaatca catgaatttt atggctaagg aaaagttgtg tctgggaaaa
    23641 gtgcagggaa ttcgggctgg caatatacac cgcgtcaatg gcatcgcttt ccgccatcgc
    23701 ttccagcgac gtaaacagat gctcgacaga aaaatcattg gcgaagtgct gagcctgctc
    23761 aaggctgcgg gaatatacgg cggttaactt gtatttaccg cgctcatggg cggcctcgac
    23821 gaactggcga gtgatccagt tcgtaccaat cacagcgaaa cgtatcataa cgcgtggata
    23881 ctccataaag ggattctgtc gtcaatgtag cacgcctttt tcatcttcga cgtgcgcaga
    23941 tacgcattat tctttcgccg gacagccatg ctcactaaac ggcccaatca tgaccgatgg
    24001 cccgagatat ttcggctggc gatggaggat ttttgcgtca atcaccgcgc tgacacgagc
    24061 gagtttctcc cgcagttgag tataaaaacc gtgcttcata ttgaggtctg gcgcgttaaa
    24121 cccgatagcg tcgatgccgt actgttttgc cagccagatg gcgcgttggt tatgaaattc
    24181 ttgcgagata atggtgatat ggttttcccc aaacaccttt ttggcacgca ctaccgaatc
    24241 cagcgttgag aatccggcat agtcgcaaaa gatcactttc gccggtacgc ctttagcgat
    24301 caaagcctgc tgcatacctg atgcttcatc gtaattttta tgtccgttat cgccactcac
    24361 cagtagccat ttcacttttc cggcgtggta caacgctgct gcggtatcaa tacgtcgggt
    24421 aaagtagcga ttacccggcc tcgcccccag caataaaccc acgttacgcg ccgggacggc
    24481 gttgacatcg ccccaggtca gctgcttgct ggcgttgacc ataacgcgat cggcgataaa
    24541 aatggtcgct cctgccacaa gtaacagtaa gcaggcaatc tttagagtcc ggcgagaaaa
    24601 acaaatacgg agaagaaggc gggcaaatgc gcgcagcatg gtcgttatcc ttaacgaaag
    24661 gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat cattttcaga
    24721 ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac tcaagctggt
    24781 ggtactgcgg ctccatatgg cagcacaatt gatagaacgc tttgttatgc tctttctcct
    24841 tcaggtgcgc cagctcgtgc accacgatca tgcgcaaaaa cggttccggc gcgttgcgaa
    24901 acacggtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc acacgagaaa
    24961 cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca tacatcactt
    25021 tattgatcgg cggggcatta cgcagaaact gatttttcag atcctgagta tattgccaga
    25081 gagctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc acatcgccca
    25141 gacgctgttc gttaatcagt gttcgcacct gcgaaagcag ctgctcggga taaccctgaa
    25201 gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata ctcacgcacc
    25261 cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc acttagacaa
    25321 cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta acccgctaca
    25381 ggcgtgggaa gcggcagatg aatatttgct gcaacagttg gacgacacag aaatccccgg
    25441 cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg cggaacataa
    25501 gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga atttacgcct
    25561 aaacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact acccgcaaca
    25621 gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac agcaactgcg
    25681 tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca aagcccgtga
    25741 cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca ccaccacgct
    25801 ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc tggccgatgc
    25861 gccgcagacc gttagctgga agctggaagg tactgactgg actatccata accatgcgaa
    25921 tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac atctgccaga
    25981 gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg gtctgacgct
    26041 gcttgataaa aacccgcagg cgaaagtggt gtttgtcgat gaatcgccga tggcggttgc
    26101 ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca gcgagtttat
    26161 gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc tctgcaaccc
    26221 tccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt tccaccacgc
    26281 ccgccgctgc ctgaaaatca acggcgagct gtatatcgtt gccaaccgcc atctggatta
    26341 cttccataaa ctgaagaaaa ttttcggcaa ctgcaccact attgcgacga ataataaatt
    26401 tgtggtgctg aaagtggtga agctgggacg tcgtcggtaa gattgggagt tacctgccgg
    26461 atgcggcgta aacgccttat ccggcctacg ttcagcacgt tacccccagg ccggataaga
    26521 cgcggcaagc gtcgcatccg gcaatgtctg ctcagttctg gaatcgcgct tccataaaac
    26581 aaaaaatccc tgtcaccatc ctctacattc tcttgtttag cgtttttcta cgtttattct
    26641 tccgtcacac agataaattc atccgttgcc aatctgtcaa cggatgttat catattgcca
    26701 atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt gaaagatgct
    26761 gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac gattgctaag
    26821 ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga taacttcaaa
    26881 tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt tgtagcaatg
    26941 gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa agaatacgat
    27001 ttctttaccg ctgttcatcg tactaaggag aaaaaatgat tgcgattgcc gacatcttgc
    27061 aagcaggaga aaagctaact gctgtggcac cttttctggc gggtattcag aacgaggaac
    27121 aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct gaaaacccct
    27181 tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc gaatttgcgg
    27241 aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt acccttatgg
    27301 atcaatatgg tttaaccctt tccgatctgc cggaaattgg cagtaaatct atggtgtcac
    27361 gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg gcaacgcgat
    27421 tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg ttgcatccgg
    27481 cgcttcaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca attgcccgtc
    27541 gtgcgtccag ctccatagcc acatcgcagc cgccaattaa atgcacagtt ttcccgctat
    27601 caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc accacattgt
    27661 ccactgctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca tcgtcaatct
    27721 tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg gtgcgatgga
    27781 tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc tggagcatca
    27841 caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca ccagcctgtt
    27901 gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg ctggtggatt
    27961 cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa ccgatgatgg
    28021 caaccttgtt gcctaccggc gctttgtcgc gcagtacatc gagataactc aataccttcg
    28081 gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg aggatcgttt
    28141 catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc gtcacgcccg
    28201 tcacttcgat catccggcgg tagtagcgca gcgtttcgta aaactcctct ttgccgggga
    28261 tctgtttggc gatattaaac tgcccgccga tctcgctgtg cgcatcaaac aatgttacct
    28321 gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtcca gcgccgacca
    28381 ccgccagatt ttttttctgc acggcgggaa ggattggcat tttggtttcg tggcaggcgc
    28441 gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg caggcctggt
    28501 tgcagccaat acaagtgttg atctcatcgg ctcgccctga ttgcgctttt gacagcagtt
    28561 ccgcatccgc gagaaacggt cgcgccatcg atactatatc ggcatcgccg cgcgagagaa
    28621 tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc agcgagacgt
    28681 ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc gtggcgatgg
    28741 tcggaatccg cgcttcgtgc cagcctattc cggtgttgat aatggtcgcg cccgccgctt
    28801 caatggcctg cgccagttct accgtttcgg caaaagtgcc gccgccttcg accaggtcga
    28861 gcatcgacaa ccggtaaata ataatgaagt cgttgccgac gcgttcgcgc accgtacgca
    28921 ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg tcactacgct
    28981 gattggtgcg cagcgtcaga aattcgttga tcaaataccc ttcagagccc atcacttcga
    29041 cgccatcgta tccggcctcc cgcgccagtt gcgcgcagcg ggcgaaatcg tcgatcagtt
    29101 gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg gcctgcaatg
    29161 cggacggtgc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa atttgcaggg
    29221 tgattttgcc gccttcctga tgtacggctt cggtaatggt gcgatggtgt gggatctggc
    29281 tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct ggtgcaatgc
    29341 cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac gcagccagcc
    29401 gttcagcacc gtccgggtat tcctccagcc cggtgtgcat tgaacccatc aacacgcggt
    29461 tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag ctcataaaat
    29521 tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag ccgtcgatgg
    29581 agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg
    29641 gtaacgccgg atgcgacgct tgccgtgttt tatccggctt gggggattgc acatgtaggg
    29701 cggataaggc gttcacgccg catccgccag ccgttgcaaa ttactgctta cggaaaaaat
    29761 cgttatacaa catatacaaa tgagcggcac tccaggagaa atttggtgcg ccttgctgcg
    29821 cgcctgtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat cctttggcgt
    29881 gcctgaagaa cgtatccgcc agtttcaggg catcatcacg ataaccgtaa cgctccatcc
    29941 ctttcagacc aaaccagaac tgatccaccc atacgcgccc gcgccagtag atatcagcgc
    30001 caaaagccgg attggttaac gccgccgttc ccagcggaac aaaggtgttg aactctttag
    30061 gatcgagcat caccttcacc accgcgtcgg cattggcctg cgttgccgca ccgttaaaca
    30121 gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc gcgcagccgt
    30181 tcgccagtgg tttatcttca atacgtacgt catagtagaa ctgggtagtc gggtcgaaca
    30241 tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt ttggcctctt
    30301 ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg tacatatagc
    30361 tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg tcctgactgc
    30421 ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc gccattagcg acatatttat
    30481 ccaactgttc tttgtcgata aaaccaaaga cggcggcatc atcacgcccc gattcccacg
    30541 acgcagcgac ctgtgccgga atttccagac tgtcgtactg gcctttctcc accacgcggg
    30601 cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcact gtaaacagca
    30661 tctcgccgct ctcggtgttg tgggctttgt cgcgggtcgc gccatattcc ggcaccccgt
    30721 tgccgttatg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt ttcgggtaca
    30781 tctctgccaa ccaggcttta tcctaggtga cgttgtatac ttccatcacc gaccaggcgg
    30841 caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca cgctcggggc
    30901 taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc acgctgtcgc
    30961 caggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgata tccggattga
    31021 aatgcaccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga ttgccggaga
    31081 accagcgtcc ggtcaccgac ggcgtgacgg tgttataatt caccgcaccg cccggtgagc
    31141 gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg
    31201 catccggatt ggttaaccct ttcttcaggt attcttccca gcgttgctgc gaggcggtga
    31261 gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg ctaacttcct
    31321 gagcggtcag cagatgggaa taggtggtgt agagcgtggt cgaaccgttg atatgcgcct
    31381 tactggtaaa gcgattaccg ttgatttcag tctgcaccgg cagggattta tgcacctgat
    31441 attccgattc gccggaggtc agcagatccc aggtggcgcg cactttgcca aaggtgactt
    31501 tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg ccagcaatgg
    31561 ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt tcgccatccc
    31621 acaccagatc cagtggttta ttgctgatga ttttagtttc cagtagtgac gtgcgcggcg
    31681 tggcgaagcg cagagtcatt tcgacctgca catcttttgc tgtcagtttt tgcaccagcg
    31741 cgccgggaat actgtatgcc tccagcgtga agttgacttt cttgccgtct tgccagacag
    31801 tcaggcggtc gaaattgctg gccataaagt tgatgtattc ttccgtcagc agcgcaacgc
    31861 ccggaaagcc gcccatggtg ttcgggccgt ctggcaacag atgaccatgc caggctccga
    31921 gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg
    31981 gcgcaccagt acggttaatc acgtttttat aattgtcggc gttagcggca tgagcggaaa
    32041 aacttatcag cagagcgcag gttactggcg ttaaaatagt tttgattttc atgaaattac
    32101 agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg atcgttagag
    32161 gcatttttca tgtagaagcg gctatcaaaa gccgtggcga aacgtccgcc gccatattcg
    32221 taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt
    32281 ttggcgtagg tgtaagcggt ggagagataa acgcccttta acgcttcata catggcgctc
    32341 accatcatcc cgctttcact tcccggtacg tccagcccgt cggcacgtcc ggtatggtgc
    32401 caggcgcgag cggcaaagtt aggggtaaac aggcccgtga gatagagttg tcctgtgccg
    32461 tcggtaattt ccagaccatt catccaggtg atgccttcgg caaggtcata ctggatgtaa
    32521 ccgttcatcc attgccggat aggtgtattt gtcgtcgtag cgatcgtact tgccaaaatg
    32581 tagccaggct ttgctttcgt catgatgtcc ggcaggtgtg gcggtgacgc tataacgaaa
    32641 gttgccgctt aagttctgta ccttcagcgc ggtcataatg tcgcgggtat tgggaatgac
    32701 gtagcccaaa tcgggtgtga agtcgcccca ccattgcagg tcatccagcg aggaatcctg
    32761 acgcagactg agcatgattt cggtgccatc attggtacga taaccgccat agaaacggtt
    32821 aatcccacct tcgaaaccac cccagctatg gtcaggcacc caggcattac cgtcgtggtc
    32881 ggcctgcacg gtccagcctt cgccgtaaat caggccgaac caggagccgt acttcatctc
    32941 caggccacct tcaatatagg tgccgtcgct gtatttatgt ttattctcac cctctaaatc
    33001 catatatcca ccaagcccta attcgccgta aaaacgaaat gaaggagaag atgtcgaggt
    33061 aattgcgggt gtttttgtgt cgtcatgttc tgccggacgt gcttcttctc ctgccgcaga
    33121 aaaggcgtag caaaacggca gagcaagtaa tgctctgcat ggtgcagtga ttaatttcat
    33181 tgtgttaatc catgagctaa agaaaaagta cagcctttta tcgttgtaat tataaaaata
    33241 taaaggcaga aactattttc cctctcctca tccaggaaga gggaaaatgc ctgctcattt
    33301 tttattagtc tgcttaatca acattgctgg ctggcgtcgc ttcaatatgg cgctcttcag
    33361 ccgttaatcc ctttatatat ttactgtatt tccaccacgc aaagccgagg aagataacaa
    33421 taccgccgac gttataaaaa atgatggtca gaatattcgc gccagtcggg aatgtcgaag
    33481 cgacaaatcc tacggcaaat atcgcaatca gcattgaaac aacgataata ccggtgcggc
    33541 gggagcccat acggaaatcg cgtggcaggt gatctaattt ggcgcgtaaa ttcaggtaag
    33601 ccagcatgat gaataacggc ggaagcatgg acgctgcggc ggtcatatta ataatggtat
    33661 tcatcagatc ctgcacggta ttggaaccga gcatcgggat aatcatcagc gggatgacga
    33721 tcagaaactg gatccatgct gcgcgcgccg gaacgccgtt ttcgttaagt tcgacggttt
    33781 tcttaccaaa gatgccttcc gggatttcgg agaagaaaat tttcaccggc gttgcggtcc
    33841 acatcagcag ggaaccgaac attgcggtaa aggacaccag accgacaaag cgattcatca
    33901 acgcttccgg cagaccaaaa tacgccgcca tgccgtggaa tacctgcacc gatccgccgg
    33961 taaatttcaa ctctttgctg ctgacgaaga cgttaatcag caccgaggag acggaataca
    34021 gtacgccgat aaaaatcccg gcgaggatga tcactttaac gaacgatttc gaaccgcctt
    34081 tgacgtcgtt aacgtacacc gcgacggact ccgcgccacc tgcggccata aagatccagg
    34141 tggtaacgcc gaggaacgcc cagttgaagt tcgggatcat cgcgtcaacg gtgatcggat
    34201 cggcaggctg tacgccgcca accagtgccg tacctgccag taaaatgtag gagagcgtta
    34261 acagcagcat cagcgttgaa gtgacggagg taattggccc cagcattttc gccccgttgg
    34321 tggaaaccca ggtggagaag gcgaacagca ccatactgat aacggtggtg gcaaccggcg
    34381 tcataatata ttcgtagccg aggaaggcat acgaagcata agcaataacg cgcggcaaca
    34441 gcgaggtgaa aaagaacaga tttacgaacc agtaagtgta ggcagtaata aatgcccaac
    34501 gtccgcccag cgaacttttt acccacgcgt agacaccggc ttctgagttt ttatttaacg
    34561 aaacaaattc tgcgatgatc agacagaagg gaataaaata aaaaatcgtc gcgaggaaaa
    34621 acatcggtgc cgaggccagt ccaagctcaa tattattatt gataacgttg ttaaagctgt
    34681 aaacggcggc aaaagtcagc gagagcaagc cgaatttgcc gattgtatta cgtttggtat
    34741 cagacataac acatctccga aaatgtgtag ggtagcggtg gcggcaaact ccttttgccg
    34801 ccgtagttgt tatttgttat ggaaataacc atcttcgatg gtgactttga gaaccacttt
    34861 tttgaccgcg ttattgcaga taaaccgata cgcttcatgg atatcgcaaa taacgatttg
    34921 cccttcgtgg acctctacgg tttctccgca gccttttaaa tattcacggt cagtttcatc
    34981 acgataatat tccactacct gtaatgtctc tttcggcgca tattcaattt tttgctgccc
    35041 ttgcaggtag taatgcactt caaaatagcg tcgatgcccg gtaaatagcg catcggtcgc
    35101 ggagtcattt tccacacggt aggtcaatga atcaccgatg gagtgggcga cgccaggctg
    35161 aatgttgtcg atattttcaa tcgcttcaac gcagcgttgc cacttcttgc cagaggcgta
    35221 aatctggcgg aactgttcta agttatcgat gatcctcatt gcttattctc gctgtgcaaa
    35281 ttcgtggaaa agaaccctgc gccgaactca tacgacgcca ggctttgcgc ggtagcttct
    35341 ccgccagaaa ccggcagcaa cgtaaagccg tagctgaagt cacggaacca gacgcgccag
    35401 gagtccagca cctcgctgcc ccaggagttg gagccgaggc caagtagctg gtggtcgagg
    35461 ttcagggtga tgtcatcact gcgctgcagc tcgttacagt gctgggcagc gtgaatgttt
    35521 tcctgggtat agtgccaggc gctgaagtta attgggcgct gcggaaccac cagcagaccg
    35581 ttgccgtggc ggttagtcag tgccgtccag cggacatgct gacggttgcc gttgttctgc
    35641 gggaagggat agttctcgaa catggcatcg acggtgctgc gccagatatc gatgatgtta
    35701 gcctgctggc tgtcggcgta gttttcgccc ggtccacgac cgtaatacgc cacctgatcg
    35761 tattcgccgt taatccccat ggtgaaaccg atgcacggaa tgatgtgcgg atagtcgccg
    35821 taacgctcgc cggaaagcgc cacgttaacc tggccatcgg cagcgatgcg ccagatgtag
    35881 gtacagcgca tcccgaagtc aaacaccggc ggtgcaataa ctgtgcggct gatgatcaac
    35941 acttcaccat cgctctgttc tacggcaaag tcgcgcagat gttcctgcat gatctgcaaa
    36001 tgattcggct gccacaaccc ttcgtactcc tgcttgtggt tgtcgatcat cggcttgaag
    36061 aagttgatct ttggctcgcg ggtcagcagc gactcgccat ttacctgcca ggatgtcggt
    36121 ttgccactca tttttgagaa ggtgatcgcg aagttgtagc cgcgaacggt gcagctcaaa
    36181 cgatcgtctt ccagcgtcag cggacgcgca ttatttggtg cgaaaggcac tggctgcgcg
    36241 gtgttttcct tcagcgggaa ctgataagtg gcgatagaat gcccggcttc gctgtagcgg
    36301 gtgtgggaat ctttggtcac cgtaatgttg aggaacgctt cgcgggcgtc cagctgcggc
    36361 agcgtgatct gcaagggggc ttcgctgttc ggcgcaacgt cgcgcagttt aatctgctgc
    36421 gtcgcgagcg tttcaccttc ggcgcgcacc tctgcgtgca gggtgtagtc atcaagcgtg
    36481 gtaaaccaca gtttattttc gactttcaac tcgccgcgag tcagatcccg cgcgtggatt
    36541 tttaccggcg cgataacctg tttgtactct ttcaggcccg atcccggcgt ctgatcggaa
    36601 tagatcaaac catcaagaca gaagttatag ttgttgggat agtcgccgta gtcgccgccg
    36661 aatttatacc agacattgcc gtggtcgtcc tgtgcctgga tcccgtggtc gcaccactcc
    36721 cagacataat gaccctgaat gcaatcgtgc ttatagaaga cgttctggta ctccgtcagc
    36781 ccgcccggtc cgttccccat cgcatgagca tattcacaga tgatgcgcgg cttcggatgc
    36841 gggtattcac caaactcatt catcagcggc acgcgggtgt acatggtgga aataatatcg
    36901 accacttcag catcgcgatc ttcttcgtaa tgcaccagtc gcgtgtcatc cagcgctttc
    36961 gccgcatggt acatcgcgcg gatgttacag ccatagccgg attcattgcc cagcgaccag
    37021 atgatgatcg acggatggtt tttctgtgcg tggatatggc gaacaatgcg ctcgacgtag
    37081 actttttccc actgcggatc gtcggtaata cggctgatat cgccgacatt agcaaagccg
    37141 tgcgattcga cgtcggtttc cgccatcaca aacaggccgt agatatcaca cagttcgtaa
    37201 aaacgcggat cgttcgggta gtgagcggta cgcacggagt tgatattgtg ctgcttcatc
    37261 aactggagat ctttctcgac gcgatccatt ccaacggcgc ggcctttgcg atgatcgttg
    37321 tcgtgacggt tgacgccgtg cagcatcaca taacggttat tgatccagaa cagaccgtcg
    37381 cgcactttga tatcacggaa gccaacgcgt tgtggcacca cttccagaac gttgccgttg
    37441 gcgtctttca gcgtcatgac cagatggtaa agataagggg attctgctga ccattgctgc
    37501 ggctgttcga cagtaaaagc aaagctggcg ctggtcagtt tttcaattgc caaatgatca
    37561 atggcgctgc tgtgcaccac gcgttcgcca tcaaacaggg tatattccag cgtcgtgacg
    37621 acaggggagg cggcgagatt ttccagcacc acttcgcagg aaagcgtggc atcgcaatag
    37681 gcttcgtcaa agtcggtacg cacagtgaaa tcgttaatat gcgttaggtg ttttccgacc
    37741 agataaacat cgcggaagat ccccgccgac caccacatat cctggtcttc cacgtaggta
    37801 gagtccgccc actgcatcac gcgcacacac aacaggttgt cgccggtttt aaccatcgcg
    37861 ctgatgtcaa actctgcggt caggcgactg cccttgctga aacccacata ctgaccgtta
    37921 acgtagactt caaaataggt ttcgacgccg tcaaatttaa tcagcgtctg tttaccctgc
    37981 cagccgtcgc tgagggtgaa aatacgttga taggcaccgg ttgggttatc gctggggaca
    38041 aacggcacat cgatggggaa cggaaaacct tcgtcggtat attgcagttt gccgtgacct
    38101 tccatttgcc acatggcggg gacggtaata tgcccccagt cagccattaa ctcagaggtg
    38161 aaggcttctg gtacttgcag cggatggtca aaaaagtgga aattccactg accgcttaag
    38221 ggcagaaaca ggctgctggt ttcgcgggca aaggtacgcg cttgcgcaac agaatcatat
    38281 gaaaaaaagt acgcacgcgg cgcaagtcgg ttttcgtggg tgagctgaat gttttcccag
    38341 cgattcatag ggcctcccgg tagagagaaa tcggaaccat tcatggtgat ggtagtttgt
    38401 gtaattgctg gggagtttag taaaattttt actaaaaagt agcaggtaaa tgcatttact
    38461 ttaactggat cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg
    38521 tgtaagggga tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc
    38581 agcggcagcg cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc
    38641 atcatctcgg aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg
    38701 atatcgttaa cgctgataag cgaaatatcc tgtgggatgt tcaggccacg ttcatgaatt
    38761 gcccgcagta cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct
    38821 tcccgcgcca gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc
    38881 cagatatcct cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca
    38941 cgaatatccg ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc
    39001 tggttgatat agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca
    39061 tcgtaaccgc tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg
    39121 gcagcggcgc gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta
    39181 atgtctggta atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac
    39241 tgagtttcaa tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc
    39301 tggtagctgt agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg
    39361 gcgctactgg tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct
    39421 tctttcacat tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg
    39481 ccagcttcga ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat
    39541 gtgcgccccc attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc
    39601 gtttctttga cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct
    39661 ccatttttag cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg
    39721 aagggaggaa gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt
    39781 cggaaagatt gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt
    39841 tgatgtgggg aaaaaacact gcaaaccgtg accagcctgg tgccgtacta cagcgaagag
    39901 gagctgatgg ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt
    39961 gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg
    40021 ttaacgccgg agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg
    40081 acgtcgtatc gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc
    40141 agcgccttac gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc
    40201 ggttcaatgc ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg
    40261 aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac
    40321 atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa
    40381 ccatccagca acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac
    40441 acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta
    40501 tgcaggaatg ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca
    40561 atcggcatca cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc
    40621 tcatgttcgc aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg
    40681 atcatcagtg cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc
    40741 ggcagaatta cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca
    40801 ccggtttttt tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca
    40861 tgacggaacc ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc
    40921 gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg
    40981 ccgcgcggcg actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg
    41041 ctattacaga agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc
    41101 agcatagccc gtaacggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga
    41161 ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct
    41221 cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgtatttaa acctcccgct
    41281 tgccactcta cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac
    41341 cccggattga catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc
    41401 tcatgatcca gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc
    41461 gatgcgctcg aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac
    41521 cgatttaagt attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac
    41581 acaaatgcgg ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt
    41641 tgcacggttt ttgtcgggta tggatttgat gattgctgcg caaatgcacg ttttcagggc
    41701 aataattgcc tgatttttgg gcaagataat taacgttttc gtatagtaac agagtgaaag
    41761 tattttaaat caaagaatta gatttgtgct ttaatcgcaa attgcgatct aaatcaaatt
    41821 aatcggttaa agataaccgc agcggggccg acataaactc tgacaagaag ttaacaacca
    41881 tataacctgc acaggacgcg aacatgtctt ctcatccgta tgtcacccag caaaataccc
    41941 cgctggcgga cgataccact ctgatgtcca ctaccgatct gcaaagctat atcactcatg
    42001 ctaatgacac ttttgtgcag gtgagcggct ttaccttgca agagttacaa gggcagccgc
    42061 acaacatggt gcgtcacccg gatatgccaa aagcggcgtt tgcggatatg tggttcaccc
    42121 tgaaaaaagg ggagccctgg agcggcatcg tgaaaaatcg ccgcaaaaat ggcgaccatt
    42181 attgggtgcg ggccaatgcg gtaccgatgg tgcgcgaggg aaaaatcagt ggctatatgt
    42241 cgattcgtac ccgggcgacg gatgaagaga ttgcggcggt ggagccgctg tacaaagcgt
    42301 tgaactccgg acgtaccagt aagcgtattc ataaaggcct ggtggtgcgt aaaggctggc
    42361 tgggtaaact gccttcatta ccgcttcgct ggcggacgcg tggagtgatg accctgatgt
    42421 ttatcttgct ggcggccata ctttggtttg ttgctgcccc ggtggtgacg tatttcctct
    42481 gtgtgttagt ggtattgttg gcaagcgctt gttttgaatg gcagattgtg cgcccgatag
    42541 aaaatgtcgc ccgtcaggca ctgaaggtgg cgaccggaga gcgtaatagt gttgagcacc
    42601 tgaatcgcag cgatgagctg gggctgacat tacgcgcggt agggcagctt ggcctgatgt
    42661 gccgttggct aattaacgat gtctcaagcc aggtgtccag tgtcagaaat ggcagtgaga
    42721 cgctggcgaa aggcaccgat gaactgaacg aacataccca gcagacagtt gataacgttc
    42781 agcaaacggt ggcgatcatg aaccaaatgg cggcgtcggt gaaacagaac tctgccacgg
    42841 cgtcggctgc cgataaactt tctatcaccg ccagtaatgc ggcagtgcag ggtggggagg
    42901 cgatgaccac ggtgatcaag acaatggacg atatcgccga cagtacccag cgcattggca
    42961 ccattacttc gctgattaac gatattgcgt ttcagaccaa tattctggcc ctgaatgcgg
    43021 cggtggaagc ggcgcgtgcc ggcgaacagg gcaaaggttt tgcagtggtg gcaggggaag
    43081 tgcgtcattt agccagccgc agcgctaatg ctgccaacga tattcgcaag ctgattgatg
    43141 ccagtgctga taaggtgcaa ttcggttcgc agcaggtaca cgccgccgga cggacgatgg
    43201 aagatattgt ggcacaggtg aaaaacgtca cccagttgat cgcccagatt agccattcaa
    43261 cgctggaaca ggccgatggg ctttccagcc tgacccgtgc agtggatgag cttaacctga
    43321 tcacccagaa aaatgccgag ctggtggaag agagtgcgca ggtgtcggcg atggtgaaac
    43381 accgcgccag ccgactggaa gacgcggtga cggtgctgca ttaatcggta tgccggatcc
    43441 ggcggttgga gcacaatgcc tgatgcgatg ctggcgcatc ttatcaggcc tgcggggtgt
    43501 gtagcgggtg taggccggat aaggcgcgtc aagcgtcgca tccggcaatt gcaccgcgcc
    43561 actggcggat gcggcgatta atccagttgc gtaatatcaa aagcggcgcg gtcgatcacc
    43621 gcaatgatct tttttatttg tgcatcactg atatcgctct ggttgacgcg taaatccagc
    43681 accgctttaa aattatccag cgcccgcttc atttgcgggt tctggcgcag cgccgcgcca
    43741 acgcaacgcg ctttgatgcg ttcttcaatc atctccacct gttcgcggtt ttcttccagc
    43801 cactgcgcgc cttgttcggt cagcgcaatc tgcttcttac caccttcctc ttcgcggatg
    43861 gtaatcagcg actgctcctg cagaaaatcc agcgtcgggt agatgacgcc cgggcttggg
    43921 gtgtaattcc cctgggttag attctcaatc gctttaatca attcgtaacc gtggctgtca
    43981 tcgcgcgaga gaatatccag aatcaccaga cgcaattcac cgtgaccaaa gaaacgttgc
    44041 cgacgaccgc cgccgcgacc gtggcgacca ccgcaacatt gaccgtgttc atgctggtga
    44101 ccgtgtccgc agtgctcgtg ttctgacttc tcacctttgc agcagccctc atggcgtggc
    44161 tggccttcat gtttacaaca cccttcgtga tgatggctca tatcagcctc cttttgcttg
    44221 attagatata tctaatttat atctgatgtg agcattactg caagtacaaa aatgattttt
    44281 aaacgtggaa gcacctcaca tctaggccgg ataagacgtt cacgccgcat ccggcagcat
    44341 tgctatggca ctgaatgcct tcacatactt cagacatatc agttctacaa atcgcttgca
    44401 tttatcatga ttaacaatca ttatcatttg cgaattttat ttagatatat ctgattacca
    44461 tcacgaaggc gataacaatg aataacaccc cccgctaccc gcagcgcgtt cgcaatgatc
    44521 tgcgcttccg tgaactgact gtgctacgcg ttgagcgcat cagcgccggt tttcagcgca
    44581 ttgtccttgg cggcgaggcg ttggacggtt ttacatcgcg cggctttgac gatcacagca
    44641 aactcttctt tcctcaacct gacgctcact ttgtgccgcc aacggtaacg gaagagggca
    44701 tcgtctggcc ggaaggacca cgcccaccgt cgcgtgacta tacgccactg tatgacgaac
    44761 tacgccatga actggcgatc gatttcttta ttcacgacgg tggggtcgcc agcggctggg
    44821 cgatgcaggc gcaaccgggc gataaactta cggtggcagg tccgcgcggt tcgctggtgg
    44881 tgccggaaga ttacgcgtat cagctgtatg tctgcgatga atccggaatg cccgcactgc
    44941 gccgccgcct ggaaacgttg agcaaacttg ccgttaaacc gcaagttagt gcgctggtta
    45001 gcgtgcggga taacgcctgt caggattatc tcgcgcacct tgatggtttt aatatcgaat
    45061 ggctggcaca tgatgagcag gcggtagatg cgcgtctggc gcagatgcaa atccctgccg
    45121 atgattactt catctggata accggcgaag gtaaagtcgt taagaattta agccgccgct
    45181 ttgaagcgga aaagtatgac ccacagcggg tacgtgcagc ggcttactgg cacgcaaagt
    45241 aactgatata taaaaagaaa aaggctgacg atttctcgtc agcctttgct atatctggtg
    45301 gcccctgctg gacttgaacc agcgaccaag cgattatgag tcgcctgctc taaccactga
    45361 gctaaggggc catggtagcg gattataaag taactccgtg tcgcaatcca gccattaccg
    45421 cgcgcctgct gtttttataa acaatgtatt ttcaatccgt tatacttttc ctggtgatgt
    45481 tagaaaggag taaatatggt tgaggatatt ttggctccag ggttacgggt cgtgttttgc
    45541 ggtatcaacc ctgggctttc atccgccggg actggttttc cctttgctca tccggcaaat
    45601 cgcttctgga aggtgatata tcaggccggg tttaccgacc gtcagttgaa gccgcaggag
    45661 gcacagcatc tgctggatta tcgttgtggc gtcaccaaac tggtagaccg tccaacggtg
    45721 caagccaatg aagtttcaaa gcaggagctg cacgcaggtg ggcgtaagct gattgaaaaa
    45781 attgaagatt atcagccgca ggcgttggcg attctgggca aacaagcata tgaacaggga
    45841 ttcagccagc gcggtgcaca gtgggggaaa caaacgctca ccattggttc gacgcagatt
    45901 tgggtgctgc caaatcccag cggtttaagt cgcgtttcac tggagaaact ggttgaagcg
    45961 tatcgcgagc tggaccaggc gctggtagtg cgtgggcgat aaaaaacgcc accga
//
LOCUS       DADLSE010000017        43486 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLSE010000017 DADLSE010000000
VERSION     DADLSE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.017"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..674)
                     /locus_tag="JN692_000950"
     CDS             complement(<1..674)
                     /locus_tag="JN692_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7263325.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     EVFDLKLGWDVTAFGGNNFAAQGLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYR"
     gene            complement(729..1670)
                     /locus_tag="JN692_000951"
     CDS             complement(729..1670)
                     /locus_tag="JN692_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7263326.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     IGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            complement(1701..2675)
                     /locus_tag="JN692_000952"
     CDS             complement(1701..2675)
                     /locus_tag="JN692_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7263327.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            complement(2672..4192)
                     /locus_tag="JN692_000953"
     CDS             complement(2672..4192)
                     /locus_tag="JN692_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7263328.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDDPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            4306..6570
                     /locus_tag="JN692_000954"
     CDS             4306..6570
                     /locus_tag="JN692_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7263329.1"
                     /translation="MAHPGRHFFASARGRLLLLNLLVVAVTLMVSGVAVMGFRHASQM
                     QELVQQQTVDDMTGSLNLARDTANVATAAVRLSQVVGALEYKGEAERLQETQRALKSS
                     LAQLANAPLAQQEAGLVTRIITRSNELQTSVGGMLERGQRRHLERNALLSSLYQNLSY
                     LRHLQKVTHAQDNILLNEMNRLIVAAIATPAPQAIIHQLVGVMSALPTHSDTPLVNTL
                     LADFNRELRKLAPLSAALEQSDLAISWYMFHIKALVAILNGDINRYAEQVATLSGQRV
                     EQSYPDLRSGERLIMIFALLAVVITAFAGWYIYRNLGSNLTAISRAMSRLAHGEADVT
                     FPALQRRDELGELARAFNVFARNTASLERTSRLLKEKTTQMEIAQTERQGLEEALLHS
                     QKLKAVGQLTGGLAHDFNNLLAVIIGSLELVNTNSPDAPRIQRAQQAAERGALLTQRL
                     LAFSRKQSLHPQAVELKTLLEELSELMRHSLPATLTIEIEAQSPAWSAWIDVSQLENA
                     IINLVMNARDAMEGQTGIIKIRTWNQRVTRSSGQRQDMVALEVIDHGSGMSQAVKSQV
                     FEPFFTTKQTGSGSGLGLSMVYGFVRQSGGRVEIESAPGQGTTVRLQLPRALTAAKNL
                     SPAAVEQTAVNGDKLVLVLEDEAAVRQTICEQLHLLGYLTLEASSGEQALDLLAASAE
                     IDIFISDLMLPGGMSGAEVVNAARKLYPHLTLLLISGQDLRPSHNPALPDVALLRKPF
                     TRAQLAQVLGQIEG"
     gene            6651..7283
                     /locus_tag="JN692_000955"
     CDS             6651..7283
                     /locus_tag="JN692_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7263330.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIACESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            7352..7681
                     /locus_tag="JN692_000956"
     CDS             7352..7681
                     /locus_tag="JN692_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263331.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN692_000957"
     CDS             complement(7828..8586)
                     /gene="phnP"
                     /locus_tag="JN692_000957"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7263332.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN692_000958"
     CDS             complement(8588..9022)
                     /gene="phnO"
                     /locus_tag="JN692_000958"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7263333.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN692_000959"
     CDS             complement(9009..9566)
                     /gene="phnN"
                     /locus_tag="JN692_000959"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7263334.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN692_000960"
     CDS             complement(9566..10645)
                     /gene="phnM"
                     /locus_tag="JN692_000960"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013573840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7263335.1"
                     /translation="MTHLYAPGKGFSDVSFDLWPGEVLGIVGKSGSGKTTLLKSISAR
                     LTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSAGGNIGERLMA
                     TGARHYGDIRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLMDHSPGQRQFANREKYR
                     EYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAALCRARQIALASHDDATHAHV
                     AESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLL
                     DILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQNPAQALNLQDRGVIGEGKRA
                     DLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN692_000961"
     CDS             complement(10672..11517)
                     /gene="phnJ"
                     /locus_tag="JN692_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7263336.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN692_000962"
     CDS             complement(11510..12574)
                     /gene="phnI"
                     /locus_tag="JN692_000962"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7263337.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN692_000963"
     CDS             complement(12574..13158)
                     /gene="phnH"
                     /locus_tag="JN692_000963"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7263338.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLKALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN692_000964"
     CDS             complement(13155..13607)
                     /gene="phnG"
                     /locus_tag="JN692_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7263339.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN692_000965"
     CDS             complement(13608..14333)
                     /gene="phnF"
                     /locus_tag="JN692_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7263340.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN692_000966"
                     /pseudo
     CDS             complement(14354..15132)
                     /gene="phnE"
                     /locus_tag="JN692_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
     gene            complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN692_000967"
     CDS             complement(15238..16254)
                     /gene="phnD"
                     /locus_tag="JN692_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7263341.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN692_000968"
     CDS             complement(16279..17067)
                     /gene="phnC"
                     /locus_tag="JN692_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7263342.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALPGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN692_000969"
     CDS             complement(17200..17643)
                     /gene="yjdN"
                     /locus_tag="JN692_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7263343.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(17901..18236)
                     /locus_tag="JN692_000970"
     CDS             complement(17901..18236)
                     /locus_tag="JN692_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7263344.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            18637..20865
                     /gene="crfC"
                     /locus_tag="JN692_000971"
     CDS             18637..20865
                     /gene="crfC"
                     /locus_tag="JN692_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7263345.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            20862..21740
                     /locus_tag="JN692_000972"
     CDS             20862..21740
                     /locus_tag="JN692_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263346.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            22004..23506
                     /gene="proP"
                     /locus_tag="JN692_000973"
     CDS             22004..23506
                     /gene="proP"
                     /locus_tag="JN692_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7263347.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            23618..23707
                     /gene="pmrR"
                     /locus_tag="JN692_000974"
     CDS             23618..23707
                     /gene="pmrR"
                     /locus_tag="JN692_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7263348.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN692_000975"
     CDS             complement(23683..24783)
                     /gene="pmrB"
                     /locus_tag="JN692_000975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7263349.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN692_000976"
     CDS             complement(24784..25452)
                     /gene="basR"
                     /locus_tag="JN692_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7263350.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTMRGFGYMLVA
                     NEEN"
     gene            complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN692_000977"
     CDS             complement(25449..27092)
                     /gene="eptA"
                     /locus_tag="JN692_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7263351.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISIRLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(27196..28533)
                     /gene="adiC"
                     /locus_tag="JN692_000978"
     CDS             complement(27196..28533)
                     /gene="adiC"
                     /locus_tag="JN692_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7263352.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDRLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(28670..29431)
                     /locus_tag="JN692_000979"
     CDS             complement(28670..29431)
                     /locus_tag="JN692_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7263353.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(29756..32023)
                     /gene="adiA"
                     /locus_tag="JN692_000980"
     CDS             complement(29756..32023)
                     /gene="adiA"
                     /locus_tag="JN692_000980"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7263354.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDDAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(32222..33130)
                     /gene="melR"
                     /locus_tag="JN692_000981"
     CDS             complement(32222..33130)
                     /gene="melR"
                     /locus_tag="JN692_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HAY7263355.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            33413..34768
                     /gene="melA"
                     /locus_tag="JN692_000982"
     CDS             33413..34768
                     /gene="melA"
                     /locus_tag="JN692_000982"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAY7263356.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            34883..36292
                     /gene="melB"
                     /locus_tag="JN692_000983"
     CDS             34883..36292
                     /gene="melB"
                     /locus_tag="JN692_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7263357.1"
                     /translation="MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLF
                     LVARIWDAINDPIMGWIVNATRSRWGKFKLWILIGTLANSVILFLLFSAHLFEGTTQI
                     VFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTL
                     PFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHLTLKA
                     IVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANL
                     VTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGT
                     ALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGY
                     VPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKV
                     PPEPVHADIPVGAVSDVKA"
     gene            complement(36431..37060)
                     /locus_tag="JN692_000984"
     CDS             complement(36431..37060)
                     /locus_tag="JN692_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7263358.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(37183..38829)
                     /locus_tag="JN692_000985"
     CDS             complement(37183..38829)
                     /locus_tag="JN692_000985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7263359.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(38907..40247)
                     /locus_tag="JN692_000986"
     CDS             complement(38907..40247)
                     /locus_tag="JN692_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7263360.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(40818..41537)
                     /gene="dcuR"
                     /locus_tag="JN692_000987"
     CDS             complement(40818..41537)
                     /gene="dcuR"
                     /locus_tag="JN692_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7263361.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(41534..43165)
                     /gene="dcuS"
                     /locus_tag="JN692_000988"
     CDS             complement(41534..43165)
                     /gene="dcuS"
                     /locus_tag="JN692_000988"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7263362.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            43346..>43486
                     /gene="yjdI"
                     /locus_tag="JN692_000989"
     CDS             43346..>43486
                     /gene="yjdI"
                     /locus_tag="JN692_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7263363.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         9871 a        11222 c        11910 g        10483 t
ORIGIN      
        1 tggcggtatt cgttacacaa caccagctgg gccggttcgt cttcgtcaat caggttgtag
       61 cggtccagcg gttgtaaaaa gtggttatcg tggtcgtcgt cattgacaga agagacttcg
      121 ccaaccagaa catcgccaaa accggtttcc gcccaaaagc tgtgatacag gccgggcggc
      181 aggcagatac tttcgccggg ggacaggcgt aactgactgc ctgcgacatg agtctggcgg
      241 cagccgtcga tcaccaccgt aacgtcggta tcaactgttt gctcgttgct atcggcattc
      301 cacagttcaa caatcaaatt gccgccgccg cgattgatga tgtcctcacg cttacgccag
      361 tgaaaatgca ttggcgtgac ctgcgcatcg cgcacatgca tgatcttctc ggcgtaacat
      421 ttcacatacg gcattccttt tgctgagccg ttacgcaggg taaacagcgt cagcccctgt
      481 gctgcaaagt tgttaccacc aaaagcggta acatcccagc cgagtttgag atcaaaaacc
      541 tcctcccacg cggcggtgtc gagctgctgc cattgagcag gagagaaact ggcaaagggc
      601 ggcagatgta cgtcatgttg cgaaaagaat tgccgtgtat ggccgagaat gttgtttata
      661 gcggagcgtt tcatcgaaag cgttctcctt agcggtggcc gggcagcatc gccgcccggc
      721 gtgtggactt atttcaccgt ccagcctttg taattgccga cgttgttttt atcgatcatc
      781 gtcaccggga tcagcaccgg ctctttcggc gcaggtttgc cctgcaaaat gtcataaccg
      841 atctcaaccg ctttcgctgc catcacctgt ggatcttgcg ccggagtagc gacaaacaac
      901 gagttctcac gcttgagcgc ctcttcggcg tccgggcttc catccacgcc gacaataaag
      961 aactcgttac gttgcgcctg ttttgctgcc agatcggcac cgatcgctgt cggatcgtta
     1021 atcgcaaata cgccgtcgat cttcggattt gccgccagca gcccggtcat cacctccagc
     1081 ccgccctcac gactgccttt ggcgttcttg ttgtcagaga gcaccttgat gtcaggatgg
     1141 cgtttaaatt cggtctggca gccttcgacg cggttttgca cggcggacac cggcggtccg
     1201 ttgatgatca ccacgttgcc tttgcctttc aggcgatcgg tgatgtattt acaggccatt
     1261 tcgcctgcct gggtgttatc ggaagtgatg gtggcatcgg caccttctgc cgccacgtca
     1321 accgccacca ccacgatccc ggcgtctttt gcccgtttca ccgccgggcc gatccctttt
     1381 gaatcggcag cgttaaggat gatcatatcg acgttagcgg cgataaagtt gtctatctgc
     1441 gagacctgct gacccagatc gtagccgctg gaaaccagcg tcactttgac gttatcgccc
     1501 gccagtttgc gtgcttccag ctccgcgcct ttggtgatct gtacgaagaa cgggttcgcc
     1561 aggtcaccta ccgttacgcc tatcgatttc agatctttcg cctgggcgaa cggagctgcg
     1621 gccagcagcg cgcctgcaca gagcgcggtc actaagggct tcaatctcat gctgtattcc
     1681 tcatgtgtag ggtattgttg ttatgcactt tgatgatgtc gggtacggta tttgtcgata
     1741 agcaccgcaa tgatgatcac cgcccctttg atcaccagct gccagaagta agagacgccc
     1801 attaaggtca tgccattgtt gagcgtggcg ataattagcg cccccaccag cgtgccggtg
     1861 atggtgccga tgccgccgac aaaactggtg ccgccgagga tcaccgccgc aatggcatcc
     1921 agttcgtaac ccatacctaa gttgccgttg gcgctgtaca gccgcgaggt gctcataatc
     1981 ccgccaagcc ctgaaagcag gccgctcatg ccataaacaa acagcagcac cagccagacc
     2041 ttaatgccgg taagacgcgc cgcctgcata ttgccaccga cggcgtagat atgaacgcca
     2101 agcgtggtgc gacgcagaat aaaccagcac agcacgatca ccgccagcgc aatcaccacc
     2161 agccacggga ccggcccgag atagttattg cctatccact cgaaattaat gttggagttg
     2221 ataaccgtgg tgccatcagc cagcagatac gccgccccgc gcagcgccgt ataggtgcca
     2281 agcgtgacga taaacggcgg caatccggca aaggccacca gcgcgccgtt gaacagcccc
     2341 agcagcaggc cgagcatcag cgccgccggg atagacaaca tggcgaactc cgggctaagg
     2401 gagacaacca ttgccgccac cgccgtggtg ccgaggattg aacctacaga aagatcgatc
     2461 ccaccggtca aaatgatgaa ggtcatcccc gccgccagca caatgttgat cgacgcctgg
     2521 cgggtaatat ttagcaggtt gctttcggta aaaaagtttg gcgcgataaa gccaaatacc
     2581 gctacgatca ggatcaaaat gggcaaaatg ccgaccgttt gcatcagatc gctcatcagc
     2641 atttttttgg cagaggtttt tgcagaggtc gtcatgaggt caccgcctgg gaatgggcgt
     2701 cgttgacgcc agtcgctaag gtcataatgt tttcctgggt gatgtttttg ccatttagct
     2761 caccggcaat gctgccttcg cgcatcacat ggacgcgatc gctcattccc actatctccg
     2821 gcagttcgct ggagatcatc aggatcgcca cgcccttgcg cgccatctcg ttcatgatcc
     2881 ggtagatctc actttttgcg ccaacgtcca cgccgcgggt gggatcatcg agcagtaaaa
     2941 tgcgcgggcc aatcgccacc cagcgggaga tcaacagttt ttgctgattg ccgccggaaa
     3001 gcccgcccgc gcgtacctgg gcatgaggca cgcgaatgtt gagcaactta atggcgtcat
     3061 cggaaatggt ttgcgctttt ttgcgattga gcattcccca gctggcatcg cgctccaggg
     3121 ttgccatagt gatgttttcg actgccgcca tttcaaggaa taagccttgt tctttgcggt
     3181 tttcggtgag aaaaccaatg ccaagatcga tggcttcgcg cggggagtgg atcaccaccg
     3241 gttcgccatc gacttcaatc attccgcctg ttgctttccg cacgccgaaa attaactgcg
     3301 ccagttcgga acgtccggct cccaccagcc cggcgaggcc aacaatttcg ccggaaagca
     3361 cctgcaggca gctcggctgg actttgccgc cgtcggtcag gtggtgaaca ttcaggcggg
     3421 ctttaccgag cgggatatcg cgctctttat tgaacagatc gctcagtggc ctgccgacca
     3481 tcatccgcac cagctccccg gcattgaggt tatcgcgggt caggctgcca acgtattgcc
     3541 cgtcgcgcag cacgctgacg cgatcggaaa gctcatacac ttccgccata cggtggctga
     3601 tgtagataat cgccatccct tcatcgcgca gccgcataat cagttcaaaa agacggtgag
     3661 tttcacggga ggagagggca gcggtgggtt cgtccatcac cagaatgcgg ctgttgcgat
     3721 gtagcgcgcg ggcgatttcg acctgctgtt gttcggcaat ggtcagcgtc atgaccttat
     3781 cgctggcgtt aaactgcgcg ccgaggcggt cgatcactct ttgcgcctgc accagcatct
     3841 cttttcgctg caccagcccg ccgtgggaaa gctcgctgcc gaggaaaata ttttctgcca
     3901 ccgtcaaatt gggtgccagc tgcatctcct gataaatcag cgtaataccg gcgcttaagg
     3961 catcttttgg cgtgcgaatg tgaaagggct tgccgtctat caggatctcg ccgctggtgg
     4021 cggtatacgc ccccgccagc accttcatca gcgtgctttt tcccgcgccg ttttccccca
     4081 tcagggcatg gatctcgcca gggtagaccg tcaaatccac ccctttgagc gcataaaatt
     4141 tgccgaaggc tttggcaata ttgcgcatct caagaaccgg cgtgctgttc atggtggact
     4201 cctgcgaggt tgtcgatatc agcactccat cacaggaaag taaatgcaaa atgtcagaag
     4261 ccgttcatat ttgtcatatt gcgatttttc ttcacggagc gtgatatggc gcaccccgga
     4321 cgtcactttt ttgccagcgc gcgcgggcga ttgctgcttt tgaatttgct ggtggtggcg
     4381 gtgacgttga tggtcagcgg cgtggcggta atgggctttc gtcatgccag ccagatgcaa
     4441 gagctggtgc agcagcaaac tgtagatgat atgaccggca gcctgaatct ggcgcgcgac
     4501 acggcaaacg tggcaacggc ggcagtgcga ttgtcgcagg tggtgggagc actggaatat
     4561 aaaggcgaag ccgagcggtt gcaggagaca cagcgggcat taaaaagctc gcttgcacaa
     4621 ctggcgaacg cgcctttagc gcagcaggaa gccgggctgg tgacgcgcat catcacgcgt
     4681 agcaacgaac tgcaaaccag cgtcggcgga atgctggaac gcgggcaaag gcggcatctg
     4741 gagcgtaacg cgctgttaag ttcgctgtat caaaacttaa gttatctgcg gcatctgcaa
     4801 aaagtcactc acgcgcaaga taatattttg cttaacgaga tgaaccgact gattgttgcc
     4861 gccattgcca cccccgcgcc gcaggcgatt attcatcaac tggtcggagt gatgtctgcc
     4921 ttgccaactc acagcgatac gccattggtt aatacgctac tcgctgattt taatcgtgaa
     4981 ctgcgtaaac tcgcgccact ttcggcggct ctggagcaaa gcgatctggc aatcagctgg
     5041 tatatgtttc acatcaaagc gctggtggcg atccttaatg gcgacatcaa ccgttacgcc
     5101 gaacaggtgg cgacgctctc tggtcagcgg gtggagcaaa gttacccgga tttgcgctcc
     5161 ggcgagaggc tgatcatgat tttcgccctg ttagcggtgg tgatcaccgc gtttgcaggt
     5221 tggtatatct atcgcaatct cggctctaac ctgacggcga tttcccgcgc gatgagccgc
     5281 ctggcgcacg gcgaagcgga cgtcactttt ccggcgttac agcggcgcga tgagttgggt
     5341 gaactggcgc gcgcctttaa cgtttttgcc cgcaataccg catcacttga acgaacgtca
     5401 cggctgttga aagagaaaac cacgcagatg gagattgccc aaaccgagcg gcaggggctg
     5461 gaagaggcgc tattgcacag ccagaagtta aaagcggtcg ggcaactgac gggcgggctg
     5521 gcccatgatt ttaataatct gctggcggta attattggta gcctggaact ggtgaacacg
     5581 aactcacccg acgcgccgcg aatacagcgg gcgcagcaag ccgccgaacg tggcgcgcta
     5641 ctgacccagc gattgttggc attttcccgc aagcagtcct tacatccgca agccgttgaa
     5701 ctaaaaacac tgctggaaga gttaagcgaa ttaatgcgcc actccttacc ggcgacgctg
     5761 acgattgaga ttgaagcgca gtctccggcg tggtccgcgt ggattgacgt cagccaactg
     5821 gaaaacgcca tcatcaacct ggtgatgaac gcccgcgacg cgatggaagg gcagacggga
     5881 attatcaaaa ttcgtacctg gaatcagcgt gttacccgca gcagtgggca gcgtcaggac
     5941 atggtggcac tggaggtcat cgatcatggc agcggtatgt cgcaggcggt gaaaagccag
     6001 gtgttcgagc cgttctttac caccaaacag acggggagcg gcagcgggct gggcttgtcg
     6061 atggtctatg gtttcgtgcg ccagtccggc gggcgcgtgg aaattgaaag cgcgccgggg
     6121 caggggacaa cggtcagatt acagcttccc cgcgcgctga cggcggcgaa aaacctatcg
     6181 ccagcggcgg tagaacagac agctgttaat ggcgataaac tggtgttagt gctggaagat
     6241 gaagccgcgg tgcgccagac catctgcgag cagttgcacc tgctgggcta tttgacactg
     6301 gaagcgagca gcggcgaaca ggcgctggac ctgctggcgg catcagcaga aatcgatatc
     6361 tttatcagcg acttaatgct acctggcggc atgagcggcg cagaagtggt caatgcggcc
     6421 cgcaaacttt acccgcacct gacgctgcta ctcatcagcg gtcaggattt acgccccagc
     6481 cataaccccg cgctgccgga cgtggcgctg ctgcgcaaac cctttacccg cgcgcagctg
     6541 gcgcaggtgc tggggcaaat tgagggatga ttttcagaat ttatcaagag gctcgaagca
     6601 aacgtgctgc tattgtgagc aattctgttc tgaattgaag tgatttacat atgatcgtga
     6661 tgccaaccac ctatagccct atgacggttg ctcgttcttt tcgcgtggtg gaagagatcg
     6721 agctttctgg acgtctgtta caggtaattt atgacaacca gacgccaaga gcggccatca
     6781 ttgaagcgga cattgaagtt tttgcaggcg tgccgcgtca tcgggtactt gccgctctcg
     6841 atctgcagcg taaaaaagag ttcggcagcg atatcattgc cgttgaacgc tggtgggaag
     6901 actcggatct ggtacaggtg gaaggtattt gtgtctctcc aggtgagcgc gataagggtc
     6961 tggcaactcg gctgtatgaa gcgctaatcc tgcaatgcgg tatcacgcta atcagcgatt
     7021 ttgatcaata tgatggcggg aagatgctct ggcagaagat cgcgtgcgag tccgatcaga
     7081 tttccgtgtt tgtattagac actgaacaag gggcattttg gccctacgat ggcacgaaag
     7141 tgatctacga tggcggctgc attccggaag agcgtatctg gagtatttca ccggatcaaa
     7201 aatgtcatgg cattgtattg gtggcggaaa ataaacagcg agctaaccag ctaatggaag
     7261 agtctgcgcc ttaccgaatt tgagattact ccgctactcc ggtcattcac actcaactaa
     7321 cgttaagcca gtccatcgca gagactggct tatgaaacct tactccacgc tttttctttt
     7381 caccctgctc acgctgtcca ccgttccggc ccaggccgat attatcgacg atactattgg
     7441 taatattcag caggcgatta atgacgctta taactctgac cgtgggcgag actatgaaga
     7501 ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat cggcgcagac aatatgacga
     7561 ccgccgccgc cagtttgaag atcgccgcag gcagctggac gatcgccagc gccagcttga
     7621 tcaggagcgt cggcagctag aagatgaaga gcggagaatg gaagatgagt atgggcgatg
     7681 aggttgggta tggagcaggc atcgcccctc accccaatgg ggagagggag aaaaccagcg
     7741 caatattcaa tatcgagcac aatcgctccc ctcgcccctc tggggagagg gttagggtga
     7801 gggggccagc gcgcacattc cctctcatca cgccacccca atctccatcc catcaaaccc
     7861 cgcctcaaac cccgacggca gtgcgttttc tatcagccac gcatcaaact ggtggctgat
     7921 atgggtcaga atcacccgtg gcgagcggat aacctgattc agcgcaagca cggtatttaa
     7981 atcacagtga ttacgcggcg catccgcgcg cggcgggtgg ctgcaatcaa tcaccattac
     8041 ctgtggctga ttattgcgta aaaatttcag cgttttttcc ggcaggccag cggtgtcaga
     8101 tagccacgcc acccggctat gtgccgtttc cagcagataa ccgaaggtca gttttgagtg
     8161 gttaagcggc agcggcgtga cctgcaaccc ctgcaaatca aacaccacaa acggttccac
     8221 tgtgtggctg aaatcaagca ggcccggatg tttaaacaga tcgtcgcaac cctggtcatc
     8281 cggcgggccg tagatcggga tcggatcgcc cacgccccag cgcagcggaa acagcccctg
     8341 ggcgtgatcc atatgataat gcgtcagcaa aaactgctgg aacgatccgg gcgaccagcg
     8401 atcggcgaga tcgtgcagcc cggcgtcgat cagggtgatt gcgtcgttaa acttcactac
     8461 gccgctgcat ggctggcggc gatactgcgg tgagcgccgc gctcggtcgc aggccgcgca
     8521 ctcgcagccc catgccggaa cgccctgtgc gccgccagtg ccggtgagcg tgagggtcag
     8581 gctcatgtta cagcaccttg gtgaagcgga agtgactctg ctcgtagcct tctcgcagat
     8641 agaaacggtg cgcgtcgtgg cgtttcacgt tggtcgaaag cgcggtcatt tcggccccgg
     8701 cctggcgggc ttcttcttct gcccatgcca gtaatttact gccgacgttc agaccgcgcg
     8761 cctgcggcat caccaccagt tcctgaattt cgccgatcca gttgacatga tgcagatgaa
     8821 actgcaaatg caggccgatc atgctgacaa cttcgccatc cagcagcgcc agatggtagc
     8881 gcatgtttgg gtcgcgcagg ttggcgttaa agcccacgcg aaacgcatgg tggtcaaact
     8941 ccgcctgttt tagctcacaa atcagcgcgt aaactgcgtc ggtgtcgtac tgcgtagccg
     9001 ggcgaagctc acaagcaggc atggtgtttc tccttctgat gaatcagcgt cagcagcttg
     9061 tcgaccgact ggcgcaggct gccgtcatta ttgagcgtaa gacaatcctg aggagtatag
     9121 cgggcagcgc gtgctagtcg ggcgttaatt tcactggcat tttcacgacc acggttttcg
     9181 aggcgctggc gcaggatctc cggcgaaacc tgtaagcaaa tgggcagcag cgccgattga
     9241 tagcgcgccc gcgcctgcgg cagatgggcg cgtgagccgt tgaccagcac gtcgaatccg
     9301 gcgtgcagcc agagatcaat ctcaacgccg acgccataat acagactgtt agcgtgccag
     9361 cttaaggcca acagattttg ccccgcgcgg gtaaaaaact cctgctcgct cagggcgata
     9421 tggttttcac ttccggcgct ggcgtcgcgc gtaatgtagc gatgcgccac cagtaactga
     9481 gtttgttccc gcaggcggat ttccgccagc aggctgtctt tcccggagcc ggacggcccc
     9541 attaaccaaa tcagttttcc catcatcaga acaccctttt accctgacgc cagacgtggt
     9601 cgatatgaat atgattgtcc ttgcgatgcg ccagcaccag gtccgcgcgt ttgccctcgc
     9661 caatcacccc gcgatcctga agattaagcg cctgcgccgg attttgggtc actaacttca
     9721 ccgcctgcgg cagcgtaaag cggttgctct ggtcatcggc gacgcgaaat gccgcatcca
     9781 gcaggctggc ggggtagtag tcggaagaga ggatatccag caggccaagc tgcgccagtt
     9841 cactggccgc cacgttgccg gagtgcgaac cgccgcgcac gatattcggc gcgcccatca
     9901 gtacgttcat gccatgcttg cgcgaggctt ccgccgcttc aaacgtggtg ggaaattcgg
     9961 cgatcacgct gccaagcagg tgggattcgg caacgtgggc gtgggtggcg tcatcatggc
    10021 tggcgagcgc aatttgtcgc gcgcggcaca gggcggcgat ggattcgcga ttcggctgcg
    10081 accagcgtgc ggcaagcgcc agttgctctt cttcgtactg ctgcatctgc gcatcggtga
    10141 gcgagtattt gccctgataa tattcgcgat acttctcgcg gttggcgaac tggcgctggc
    10201 ccggcgagtg gtccatcagc gtcaccggct cgcgctgcac cagtttttca aacagcggca
    10261 gcgtggtgtg atgcggcagt tcgcagcgca ggtgcagacg gtgctcggcg cggatgtcgc
    10321 cgtaatgacg ggccccggtc gccatcagcc gctcgccgat attgccgcct gccgagacct
    10381 ggcgacgcag gccgtcgagt ggatgctgat gcaccacgcc ccattcggtg cgcagcaggc
    10441 gacggcggtc ggcctcgctc atcgcataca acgaacgatt ctcgtagcga atctccccct
    10501 gctgcggcgt caggcgcgcg gagattgatt tcagcagcgt ggtcttcccg gagccggatt
    10561 tcccgacgat gcccagcact tcccccggcc ataaatcaaa agagacatcg ctaaagcctt
    10621 tgcccggcgc gtaaaggtgg gtcaggttat tgaccgaaag taacggttga ttcattggtt
    10681 ttttgcctcg ctctgttggc ggcaataatc ggtatcggag cagacaaaca tgcggtttcc
    10741 ggcgtcatcc agcaccactt cgtcgagata gctgtgggtc gatccgcaga tggcgcatgg
    10801 ctcatcccac tgctgaacgg tgaacgggtg atcgtcgaaa tcgagacttt ccacgcgggt
    10861 aaacggcggc accgcataga tgcgcttctc gcgccctgcg ccgaacagct gcagggcggg
    10921 catcatgtcc attttcgggt tatcgaattt cgggatcggc gacgggtcca tcacgtagcg
    10981 cccgttcacc ttcaccggat aagcataggt ggtggcgata tgaccgaagc gggcgatatc
    11041 ttcatacagt ttcacctgca tcacgccgta ctcctccagc gcgtgcatag tgcgggtttc
    11101 cgtttcgcgc ggctcgataa agcgcagcgg ttcggggatc ggcacctgga aaataactat
    11161 ctgatcttcg gtcagcggcg tttcggggat gcgatggcgc gtctggataa gcgtcgcatc
    11221 gtccgtacgt tcagtggtgt tcaccccggt cacgcgcttg aagaagttgc gaatcgacac
    11281 ggcgttagtg gtgtcgtctg cgccctggtc aatcaccttc aacacgtcgc tttcgccaat
    11341 cacgctggcg gtgagctgaa tgccgccggt tccccagccg tagggcatcg gcatctcgcg
    11401 cccgccaaac ggcacctgat aaccggggat agccaccgct tttaagatgg cgcggcggat
    11461 catgcgtttg gtctgctcgt cgaggtaggc aaagttgtag ccgctcagat tagccatggt
    11521 tctgctcctg ttgcagacgt ttgagtagct ccagttcggc ctggaaatcg acgtagtggg
    11581 ggagtttgag gtgcgagaca aagcctgcgg cttcgacgtt gtcggcatgc gccagcacga
    11641 actcttcatc ctgcgccggg cctgtcgcgt gctcgccgta ttccggggct tgcaacgcac
    11701 ggtcaaccag cgccattgcc atcgctttgc gctcgctcat gccgaatacc agcccgtagc
    11761 cgcgcgtgaa gtgcggcggc tcatccggcg ggtcgataaa accgttgacc atttcacact
    11821 cggtcatcag cagttcgccg acgtttaccg caaatcccag ctcttccggc acaatcgaca
    11881 cgtcgatgta accactgcgg atctcgcccg cgaacgggtg attgcgtccg taaccacgct
    11941 gggtggagta ggccagcgcc agcaaatagc cttcgtcccc gcgcattaat tgttgcaggc
    12001 gggaggagcg tgagcagggg taaaccggcg gcgtgcgggt gatgtcatcc ggctgtgcgc
    12061 cgctatcttc ttcaaacttc gccagcccct gacgcgccag caggctgaaa acgtgcggcg
    12121 acggctgttg ttcgctgtcg gcggtggtca gcgtcggcgc ttcaccgttt gccagcaggg
    12181 tgaaatcgag caggcgatgg gtgtagtcgt aggttgggcc aagcagctgg ccgccgggaa
    12241 tgtctttata aaccgccgaa atacgccgtt cgagacgcat ctcggtggtg tcgagcggct
    12301 cgcttaccgc cagtttcgcc agcgtggtgc ggtaggcgcg cagcaggaaa attgcttcaa
    12361 cgttatcgcc gctggcctgt ttcagcgcca gcgccgccag ttcgcggtcg gcaacgccgc
    12421 cttcggtcat cacgcgatcc accgcgagat taagctgctg ttcaatttgg gcgacgctca
    12481 gttcgggcaa atcggtatcg ccccggcgtc ggctctcttg cagggcgtgg gcggcgtcga
    12541 tcgccttttc gccccctttc acggcaacgt acatcagcac acctccacat gagtggttcg
    12601 cggaatagcc agcaggcgtt cgccgcaggt caggatcagg tcgatgccga gcgggaacgg
    12661 gtgcgggcgc tcggtgagtt cgtgcagaat gcactccggc agctgcggag cgatcattcg
    12721 ttcttcggca atacccgcgc cggtgagacg caacatgcgc ccgccgctca ggctggcgac
    12781 ctgtaaaatc agcgtcgcgc ccgcttccgg cgcaacggcg gtgccggtgg aaagggcttt
    12841 gagctgtttg ctggaaattc cgtcatccgt caccgcgaag gtcgcctgtt ccggctggct
    12901 gaccagcggc gcgttggtat gaaaacgcag gctctgattg acgatatcgt tactcaatgg
    12961 cgcggcaagc cacaccggcg tgtcgttatc agccagcgtc agcagcacgc tggtggtggc
    13021 gatattcagc ggctgccagc cgcgcttgag ctgatgcagg gcgacaatca cgcccggctc
    13081 gcttatggcc tttaacaggc gacgaaaact gtgctgggca tcctgcacgg gaagcataaa
    13141 agcggtttcc agggtcatgc gttgtctccg cgaaccatcg taaagaagtc gacccggctg
    13201 gcgttcactt cggcctggcg tgcggcaatg cgtgccatac ggtcagcgtc cagcggggca
    13261 ataagggttt ctgataagtt ttgaaagtgg cgagattgct gcatcagcgc atcaatcagc
    13321 gcgcagcgtt cggtgtgctg tttatcacgc cccagcaccc agctgtagcc gagggtgccg
    13381 tcagtcaggc gtacggcggc gcgggtcagc gtggcgtcgc cggcaaaaaa gcgttcgccg
    13441 gtgccgccca ttcgcgcctg aatctgtacc aggccggttt cggcggtgcg gatcacttca
    13501 tagtcggcgg tgatgttcag cgcgttcagg cgggctgcca gttcagccgg ttggctgtgc
    13561 gccagtacgg acatccagtg ctggcgggtc gcggtatctg cgtgcattca gtgctccata
    13621 gtgaattcaa tcatgtcggc gcgcgtcagg ctgacggagt actccgccgg gctgctttca
    13681 ccgtcacggt ggttaagggt gcgcacgcac agcagcggcg acatattcgg gatttcaagg
    13741 cgctggcact ctttggcctg ggcgcggcgg gcgctgatcc gcgtctggct gcggcgcagc
    13801 gcaattccgg tttgctcgcg cagaaaatcg tgcagcgaac cgctgtcgaa gcgttgcagc
    13861 gtcggccaaa gggtgaggtc cgcaaagtag tggtcgatta aacagagcgc gacgccgttg
    13921 acccggcgca gggtgcgcaa atggataacg ttctcgccct cggtaatccc cagcgcgtca
    13981 gcaacgtgac cggacgcggg gcgcaatacc gacagcagtt tttcgctggt gggatggctg
    14041 ccctggtcca gcaggttctg gctaaaacgc gcctgggcgt tgagcgggta atcgaacggg
    14101 cgcatcagca ccagcacgcc gacgccctga cggcgctgta cccagccttt ttccaccagt
    14161 tggtcgatgg cgcggcgcag ggtgtggcgg ttcacctcaa agcgcgccgc cagttgctgc
    14221 tcggcgggaa gatagtcgcc gcagcggtag tgttgacgaa gctcctgctc aagttttgcg
    14281 gctatctcct gatagcgtgt tgggtagctg gtcggatgtg tagacaagtg catagatatc
    14341 aatgcctcgc ttatcagata aagtgcttac gcaaccgttg agagaggaaa tccagcaggc
    14401 tgaccgtgac gatgataagc accatcaggg cgcaggtttg ctggaactgg aaaccgcgaa
    14461 tcgcttccca cagggtgacg ccgatcccgc ccgcgccgac catgccgacg acggtcgccg
    14521 agcggacgtt ggattcgaag cgatgaggga gtaggagatc agcagcggca tcacctgcgg
    14581 cagcacgccg taaaggatct cttcgagttt gttggcgccg gtggcgcgaa tgccttccac
    14641 cgggccgggt tcgatcgctt ccaccgcttc ggaaagcagc ttggagagca cgccggtggt
    14701 gtggataaac agcgccagca cgccagcgaa cgggccgagg ccgacggcga ccacgaacag
    14761 catggcgaag accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gaacgggctg
    14821 gtaaacccac cacggcacca agttttcggc gctcatcaga ccgaagggga tggagagaac
    14881 cactgccagc gcggtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta
    14941 atcctgccac tggctgaaat cgggcgggaa gaagtcggca gcgaaggttg ccatgttgcc
    15001 gccgtcttta atcagcgtaa gcggggccat ttccgcgccc tgccacgaga cgaccagcac
    15061 ggcgagaaca acggcccagc tcagaagcga gaaccagcta cgcttgggtg gggcgatggt
    15121 gatggtttgc atgtttggct ccggtctgta ggccggataa ggtgcttgca ccgcatccgg
    15181 catcaacgcc tgcacattgt cggatgcgac gccggggcgt cttatccgac ctacgattta
    15241 ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc
    15301 atccagctgc gcctgaatct cggtggtttt cgccagcttg tcctgctcat tcagcccttt
    15361 attgcctttc acgctttgta tctctttaaa cagtgcgagc tggcgaatcg gcaccagttg
    15421 caggtcgctg gaagcgcgga acggcgccca gcccaggcgt tccagcaccg ctttctcttc
    15481 cggcgttttt ccgtagttca taaagaagtc gtagatcttg tctttggtag tttcggacag
    15541 attcttgcgc cagacgatcg gatcgcctag gatcagcggc gatttccaga tcaccttcag
    15601 ctctttaagc ttctccggcg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt
    15661 ggcaacatcc acctgcttgt tggcgacggc cagcgcgttg gtttcatgcc cggcgttgac
    15721 ggtgcgcttg aagtcgctgg cggagatatt gtttttagcg aagacgtagt aaccggggac
    15781 gaggaagcca gaggtggagt taggatcgcc attgccgaag gtgagatctt tccgcttcgc
    15841 cagcagatcg ttcaggttgt tgatcgggct gtctttgttg acgatcaaca cgctccagta
    15901 acccggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccattgg cgcgatccac
    15961 cgcttccatc gccgacaggt tgccgtacca ggcaatatcc actttattga agcgcatccc
    16021 ctggataatg cccgcgtagt ccggggcaaa gaaggcgttg accttcacgc ccagtttctt
    16081 ctccatatcc tgtaaaaacg gcgtccattg cggtttcagg ttttgctgtg attccgttga
    16141 aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgtgccgggg ttaacagggt
    16201 gctgaggctg aacatgctgg tgaaggccag cgaggcaatt atcttagcgt tcattcgttt
    16261 tcctcaatga tggggatatc aggcagcttt cgcgttctct tcgatgcggt taatgctgcg
    16321 gtagagatgg tcaaaacgtt cgttatcaaa cagttggctg ctgccgtcgt agaagacgtg
    16381 cccctgacgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag
    16441 cgtgacgacc acggtgatgc cgtcgttctg gttgatgtcg cgcagggtgt ccatcacgat
    16501 gcgcgctgat tccgggtcca gcgaggcgat gggttcatcg gccagaatca ctttcgcctg
    16561 ctgcatcagc gcacgggcaa tcgccacacg ttgctgctgg ccgccggaga gggtggaaac
    16621 gcgctgatgg gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg cgcgctgttt
    16681 ctgctcgccg gtgaaccagc taaaacaggt gcgccagaac ggcgtgctgc cgagcgcgcc
    16741 aatcagcacg ttctccagta cgctcaggcg gttcaccagg ttgaattgtt gaaagatgta
    16801 gccggtgttg gcgcggcttt tgcggatatc gcgggccagg cggccttcgc gctggacagt
    16861 gcggcccagt agctcgatat ggctaccggc ggatttatcg ccggtaatca aaccgcttaa
    16921 atgacgtaaa agggtggatt tgccggaacc cgacggccca ggcagagcca ccatttcact
    16981 gtgatgaatg ttcagatcaa ccgcatgcag cgcttgatgc tgattgaagg ttttggcgag
    17041 cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt
    17101 ggcggattat tgtgacgatt tggttaatta aaagtgacta acagatgaag agttaacggg
    17161 aattcgatga cagtgcggga gggcgggcct cccggcgggt tattgcgttg gttgttgttt
    17221 gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccaa agccatgcgc
    17281 ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaaggt tatcaaacca
    17341 gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc
    17401 ttttcctgac ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc gcacgttggc
    17461 atgagcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    17521 cgctgatttc ggcatttcgc cgaagctgat tttatagagc agttccgcgc ccaacgtacg
    17581 ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg gacttaacgg
    17641 catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg
    17701 agaggctgcc tgataagacg cgcaagcgtc gcatcaggca gtcggcactg ttgccggatg
    17761 cggcgtaaac gccttatccg gcctacggag ggtgcgggaa tttgtaggcc tgataagacg
    17821 cgcaagcgtc gcatcaggca gtcggcatcg ttgccggatg cggcgcgagc gccttatccg
    17881 gcttatcgat cacaatacaa tcagttcttt ttcacaaact cagatttcag tttcatcgga
    17941 ccaaaaccgt cgattttgca atcgatgtta tggtcgcctt caaccaggcg gatgtttttc
    18001 actttggtgc caattttcag catcgaagag ctacctttca ccttcagatc tttaatgatg
    18061 gtaacgctgt cgccgtcagc cagcagattg ccgttagcat ctttaacgat cagctcgtcg
    18121 ctttcctgtg caggttctgc gtcgttccat tcgtaggcac attccgggca gatgtacatg
    18181 ccgttatctt cgtaagtgta ttcggagttg cattttgggc agtgtggtaa tgacatgtgg
    18241 atttcctcaa aagtcagcag ggcaaaagcg ccaaaaaacg gcagattgcc gaaaaaggcc
    18301 gcaattatac acaaaatccc tgagtttgtc gggggtactg ctgattttag acgacgatcg
    18361 ttttcatttg aaaggaaacg caaaaagaaa ataatctttt tgcctctatt tattatttaa
    18421 gataatgtta atgctctact tgttcttttt tttattcaca tgatattttt atctttctga
    18481 cgttttaaac gcatcagaag acgttattta ttctgataaa aaaaacacac taaatataaa
    18541 attatttctt gcagtaatta taaaagccga aaacagaact caaaaacaat ctggctaaat
    18601 aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
    18661 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
    18721 aaaatgcctc tctctgtacc cggagacgac gcgccacaac tggctttacc ctggagtcag
    18781 cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgcgtaaaat ttcccgactg
    18841 gaaatggtgc tggcaattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc
    18901 attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct gccgacgctt
    18961 attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt cgcgccaatc
    19021 gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
    19081 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
    19141 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
    19201 gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
    19261 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggaaagttat
    19321 cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
    19381 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
    19441 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
    19501 tcggtgccgc tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
    19561 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
    19621 cagatattcc cggtgtcgtc gatgtggggc tacctggcga atcgggcgcg ctatgagtta
    19681 gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga ttttgcccat
    19741 gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca tattcgtcat
    19801 gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc gttgcttcat
    19861 gccgcttacg ctaacgcctc attgtatgct ctgcgatctg ctgcgcataa actgttgaat
    19921 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
    19981 gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt gcaactcaat
    20041 caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
    20101 cactttctgc gtcaacagca agatgcggtg aatgcgcagt tagccgcctt gtttcaggat
    20161 gattcggagc cgttaagcga gattcgtacc cgctgtgaga cactgttaca gacggcgcag
    20221 aacaccatca gtcgcgactt tacgctgcgt tttgccgagc tggaatccac cctttgccgg
    20281 gtgttaaccg atgttattcg ccccattgag caacaagtca aaatggaatt gagcgagtca
    20341 gggtttcgtc ctgggtttca ttttcctgtt tttcacggcg tagttcccca cttcaacact
    20401 cgccagctgt tcagtgaagt catttcgcgc caggaagcaa cggacgagca gagcacgcgt
    20461 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
    20521 aatgagaaat ccccgacaga aacggttgat tacagtgtgt tgcaacgagc attaagcgca
    20581 gaagtcgatc tctattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
    20641 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac agaattacag
    20701 acgcgtttac gcgaaagtct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
    20761 cagcagcaat tgcagcagac tgtgatgact cacagctgga tttacaccga cgctcagctg
    20821 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
    20881 acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg
    20941 gcgatgatgc ccgccatcct caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
    21001 aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta aatgcgccgc
    21061 ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
    21121 cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac cgccagcact
    21181 ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
    21241 gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca ggtgatcaga
    21301 gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc
    21361 gctgttttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc tgccgtctgg
    21421 gaagcccgca agcggtggtg ttgttgctgg gtgatttgcg cgagaaagcg acacaacatc
    21481 tggcagagag tatcaatgcg gcaccgacca cgcgtcacta ttaccatcag tggtttgcct
    21541 cttcgaccgt tccgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga
    21601 ccacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc
    21661 tggggatgcc acgactctgt tcagcgcagc gtttggtggg ggcaatggtc gaggaaatct
    21721 tctctgtaaa tttggcgtaa ataatcagtt acatcaatga gtcctaaacg aaatccatgt
    21781 gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc
    21841 aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg
    21901 cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt
    21961 tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa
    22021 agtaaaaccg attacccttc gtgatgtcac cattattgat gacggtaaac tgcgtaaagc
    22081 cattaccgca gcatcactgg gtaatgcaat ggaatggttt gattttggtg tttatggttt
    22141 tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt
    22201 tgctgcactt gccactttct ccgttccctt tctgattcga ccgcttggcg gactcttctt
    22261 tggtatgttg ggcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat
    22321 gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc
    22381 accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggcg gtgaatatac
    22441 tggggcgtcg atatttgttg cggaatactc ccctgaccgt aaacgtggct ttatgggcag
    22501 ctggctggac ttcggttcta ttgccgggtt tgtgctgggt gcgggcgtgg tggtgttaat
    22561 ttcgaccatt gtcggcgaag cgaacttcct cgactggggc tggcgtattc cgttctttat
    22621 tgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag aaactccggc
    22681 gttccagcag catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc
    22741 gaaagtctcg tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg
    22801 tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt
    22861 gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat
    22921 cggtatgctg tttgtccagc cggtgatggg cttgctaagt gaccgttttg gccgtcgtcc
    22981 gtttgtgcta cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat
    23041 taacagtaac gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa
    23101 ctgctttatg ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta
    23161 cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact
    23221 ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt
    23281 agtggcggtg attggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt
    23341 gaaaggtgcg acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca
    23401 ttacgataat atcgagcaga aaatcgataa tattgaccac gagattgccg atttgcaggc
    23461 gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg
    23521 cctgatgtga cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac
    23581 gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag
    23641 tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttgccac
    23701 gttctgatct ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg
    23761 caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc
    23821 aatcccgcca taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact
    23881 ctcatcaata cctggtcctt catcttcaac tgccatgacc gctccgccat cttcttgcag
    23941 cttaatcata atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaagtttcg
    24001 caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg
    24061 caatagcagg gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag
    24121 aatgacatct tccagcagtt ttacatgttg ataattaccg gaagaaaatg actgtccggc
    24181 acgcgccagt tgcagcagct gggagacgct ctccatcatc tgatcaagcc gtgccactaa
    24241 cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc
    24301 cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt
    24361 gctggtcagg cgactgacca gatcgttcaa cgccgaaacc accgcttcga tttcgagggt
    24421 ggcgctgtga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg
    24481 cagctccgcc agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag
    24541 gctgaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat
    24601 gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc
    24661 ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc
    24721 gatggtcaat atcagccgtt ggcgcagcga tattggtcgg cgcagaaaac gcatcagatt
    24781 caattagttt tcctcattcg cgaccagcat atagccaaag ccgcgcatgg tgcggatacg
    24841 ggctttgccc actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg
    24901 ttcattgtcc cagttataga tgtcgttgta gagaatttcc cgatgtaccg gactgcctgc
    24961 tttgagcatt aaccgtgaca gcagagcata ttctttgggc gtcagaatca actcttcacc
    25021 gcccatccat acctgacggc gacccatatt cagcgtcaga ttgccaacaa tcagctcact
    25081 ttcgccctga ttattatggc gtcgtagcag ggcgcggata cgggcgtgta actcttccag
    25141 cgcaaaaggc ttcaccagat agtcatcggc accgacatcc agcccggcga ttttgtcggt
    25201 cagcgtatcg cgagcggtga ggatcagtac cggcagggtg tattttttct gccggatacg
    25261 ggcgagaaaa tgcagtccat cttcgtcggg taaccctaaa tccagtacca ccaggctgta
    25321 atgaccgtcc tcaaggcttt gttccgccat ccgcgcggtt gtcacgccat cgcacgcgta
    25381 gccttcggtt tgcgccgcca gaatcagtcc ctgcaataac agcgtatcgt cttcaacaat
    25441 cagaattttc atttactcac tctcctgcaa gtttgcagaa tatcatccgc ggcctggtaa
    25501 tacttcgtct caacgccagt taatcccaat agcgtggaga ataaattgtc ttgtgaatag
    25561 tgttgcgttt gcgcctgttt ttgcaggcag ttctggtcaa cctgataccg cttttgataa
    25621 tcctccgaca gccacagcag cattggcacc tgtttttggc tatccggggc gatggcataa
    25681 ggcagaccgt gcagatagat gccattttca cctaacgatt caccgtggtc agaaagataa
    25741 accaggctgg tggtaaattt atcctgatgt tctttaagca gattaatcgc tttatcaaca
    25801 atatagtcga cgtaaaccag cgtgttgtcg taagtgttca ccagttgctc tttggtacag
    25861 gtctggatct cattggtgtc gcaggttggg gtaaatttcc tgaactgcgg cggatagcgg
    25921 ttgtaatagg tcggaccgtg gctgccgatg gtgtgtaaga caatcacgcc atcaccctgc
    25981 aggttattga tgtactcttc cagcccgtgg aacagcactt cgtcatagca ttcgccgttg
    26041 atgcactgat caggcagatt cagcgcggtg acgttctggt gaggcacgcg gtcgcaggca
    26101 cctttacagc cgccatcgtt gtcattccac agcacgttga tgcccgctcg ctgaatgata
    26161 tccagcacgc cttcctggtg ctgtgccagc tcttctttgt agtgctcacg cggcatatcc
    26221 gagaacatgc acggtactga aactgccgtt gccgtgccgc aagatgcggt attagggaaa
    26281 tagaccacgt tatctttcgc cagccgcggg ttagtttcac gcgggtagcc gttgagggag
    26341 aagttctccg cccgcgaggt ttcgccgaca atcaggatgg tcaaattttt acgtttttcg
    26401 ttctgcatta acgggttgcg gtgcgcgtct tcaccaattc gcaccagcgg cagatttgcc
    26461 agtcgctgat gggagtacca tgaccagctg gcaacaatgc tgttagaggg gcttaaggat
    26521 ttcaccagct ctttgttatt gcggaacaac gaggcgtagt ctttataaaa cagtgcggcg
    26581 accagcaaaa tcagcagtac agaaaccaga atattggctc cacggaaaag aacactgcgc
    26641 agacgcgagg tggcaggttt gatttttagc cagcaggcaa tcagcgcagc aagcacgccg
    26701 ctgaatccca gcgttaataa catttgcggt gtcatcagcg cataactttc tgctggagtg
    26761 gtatcaataa tattggcaat catcgagcgg tcgatgacga tgccgtaagt cattatgaaa
    26821 tattgtgcag ccgcgccaac cagaataaac aggcaggcca gtggtcgatt aagccataag
    26881 aaagagctta gtgtcaggac aatattaatc acgctgaaag cgacgaccgg catcgacaag
    26941 aaaaccagta cgttatgcag cgaatccagc ggcagcgcct gcaacacctg tttaaaaaag
    27001 gcaatattca ggcggataga gatataaaaa gcggccaaca atagccaggc gagtaaattc
    27061 aaagagggtc tttttagtag gcgcttcaac atcacggtgt ttccatcgaa caaagtgcgc
    27121 atatgctcgc aaagcaaaat taagccaacc ttaagttctt aaggttggct tttatgtttg
    27181 ctggattaag gggaattaat ctttgcttat tggtgcatct aagggatacg ggtttttatg
    27241 tagccggttg taattcagcg catacatggc ggtgatgacc atcagggtga caaatgacca
    27301 cataacctct ttcgccccgg accccaccac ggcccaaatg cagtagagga aggcaatggt
    27361 agtaacagcc agatatgccg ggcgtgcttt accaaagtga ccgtggccga gcagcagtaa
    27421 cgccgcacag gtgtaaagat atggcaccag tgtaaagatg accgagacgg aagaaaccag
    27481 accgaactct ttggtcgcgt ttggtgaaat gctgctgagc tggaagatgg tcatcaaaat
    27541 accgacgata atcagccccg ccactggcgt acccgcttta tttacacggg caaaaatcgg
    27601 tgggaacagt ctgtcatcgg cagcggcttt cgccgtttga cccgccagca acgtccagcc
    27661 gcccagtgaa cctaagcaac ccgcagctgc gcagaaggaa acaatggccc cagcggtgtc
    27721 acccaacgcc atccgtgcgg catcaccgaa tggcgaggca gaaacgcgca gtgcggcatt
    27781 agggatcatc cccataatcg cggtggtgga gagcacatag caaacggcgg caatcaatac
    27841 cccaccaatg gtggcgatag ggacattgcg tttcgggttt ttcaccacac ctgcggcaac
    27901 ggaggcactt tccacaccga tgaacgacca cagcgtaacg ttaagggtac tttgaatcgc
    27961 accgaaggtg cccaggccgc tgacgttcca tgccgccata taggtttcac cacggaacca
    28021 gaaccagcca aatacggcaa tcccgacgat ggggatcagc gccagcacag tggcaacggc
    28081 ctgcacgcgg gtgatcattt tcggaccgac aatgttcagc aggacgaaga tccacagcac
    28141 cacgacgcag gtgatggtta ataccaatgg atctttcaga atcgggaaga agtaacttaa
    28201 atatcctacg ccaatgacca ccatggcgat attgccgatc cagcaggcca gccagtagag
    28261 gacgttggtt tgataaccga gaaacgggcc aaagcagcgg cgggcgtaag cgtaagaacc
    28321 accaggactt gggtcgagga acgacatttt ggcgtatacc atcgagagcc ccagcgcacc
    28381 gataatcgtc accaaccatc cataaatggc aatcccgcca gtggaggcca ggtttgcagg
    28441 taacagaaaa acacctgacc ccataatatt ccccgacacc atcagggtga cggggattaa
    28501 gcccactttg tgagcatcag catccgaaga cataattaaa ctcctgcgaa ggcgagcttc
    28561 gtgacaataa attacgtcat atcatacgcc tgcattcgat agttgaatta ttatcgaccg
    28621 ggttatcgct gatgccgtta ttaatatagc tcttcgttaa ggttaaaaat caggcgacag
    28681 tgcgttctct gtgctgacta acataatgca gcggcgtcat accgtagaag tctttaaaga
    28741 cagaaataaa gtacgacgta ctgttgtagc cgcaggactg tgatacctga gagatatttt
    28801 taccgtccat cattaattca tttacggcat aacgcatgcg gcaggtggtg attatttggc
    28861 tataactggt gttttcgctt ttcaactttt tctttaacag acttgggcta agacataaac
    28921 aactggctac catactaaga ttccagtctt tgtgaatatc gctttcaata atttgataaa
    28981 cgctgtcact tacacaatta cgtaacatat acatcattaa tgaaaggaat tttttactgt
    29041 cgagaaaacg ggatagcacc gtgaataata atgcgcgtgt tcgttccttt tctgactcag
    29101 tttctgcggg catcatgctg tgttgcgcgg caacgcgaaa gacgtctggc gtcaggcatg
    29161 gcagggtgag tatgggcgta gcgctacgtt gccatactgg tatttgcgag agatctttat
    29221 tcagaaaccg gaggtaatct ttgatgatgt cgtgactaat atgagcaacc aaagtgttgt
    29281 taagagagga gacgtcgata atattatttt cacaatttaa taacgccata tggttagctt
    29341 taaggctaat aggttctttc ccattcaccc ttatccagac atctttttca gtcaataaaa
    29401 caatacaagg ttggtcgctg caaatcctca tatatgtact cctgagtgcg aataaaagac
    29461 cagttctcgc taaagcaaag cgataccggg aaatggttat ataattgtat gatgaatata
    29521 aacatgcata aaaataacga gttttactaa tattacaaat ttttatagta atttctcatg
    29581 tacatatttt tttacaggat aaataacagg caaaaaaaag cgcggggtgg ggccgcgcaa
    29641 aaagtatgac atttttgttt gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc
    29701 taaggccagg cggcgtaatg ttatttaaac aattacgcct tcagcggaat agtggttacg
    29761 ctttcacgca cataacgtgg taaataccgt caataatttc agtcccttca gtttcgtgtt
    29821 caaatccagg gaaatggtgg tcccaggatt gcagcgagcg taaataactt acttgcggac
    29881 tgtttttatc gccgaagttt tcaccagaca gcagcatcgg gattcctggc ggatacggga
    29941 taactgagtt tgccgcgatg cgtcctggca gattttcaat ggataccagt tcgacattgt
    30001 tgtcgacaat cgcgttgtac gcttcacgcg gggtgatttc cgccaccggc aggccggaat
    30061 aggcttcgtt caaccgtgcg ccagggttgt tttctttcag ccaggcaaac atggtgtcac
    30121 ccagatcgtg aatccccatg ttcgcgtaag tgtcaggata ttgttcaaca agttccggca
    30181 tcacctgcgc cagcggtgtg ttggcgtcat agtggcgttt gaaggagcaa agggtgttaa
    30241 ccagagtccc ccatttccca cgggttacgc ccatagagaa caggaacata atttggaagt
    30301 cagtggtgcg ggtaggcaca atgccgtggc gaccaagcca ggcagtgacc agcgccgccg
    30361 gaacaccggt ttcttccagt tcaccatctt cacccattcc cggagcaagg atgctgactt
    30421 taattgggtc gagcatactc cagttatccg gaatatcttt gaagccgtgc cagctttcgc
    30481 ccggatgcat tacccagcag tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa
    30541 agtcataggt tttgccggtt tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga
    30601 accagctacc gtcagcggtg aactctttat atagccgcgc catcgcctga cggaaatcaa
    30661 ccgcgtcgtc aatcacttcc tgtgtcagtg acaggccgct gttgccgtcc atcatcgaca
    30721 ccgccacgtc gttggatgcg cagatggcat acagcgggga ggtggtggca tgcatcatgt
    30781 aggcctggtt gaagcgggag aagttaatcg ccccacgacc ttcacgtaca tgaatataag
    30841 aagcctgtga cagcgcattc agcagtttgt ggatggagtg ggtggcgaaa acggtaggac
    30901 cgttgtgatc gccaggttcg ccgcgcatgg catagtgatc ggcatagatc gggttgaaac
    30961 gtgcatagcc gtaccaggct tcgtcaaagt gcagacgatc ggaggttttt tccagcagat
    31021 cctgcgcttc tttagcgtta taacacacgc cgtcataggt gcagttggtt accacgcagt
    31081 aagacggttt ttgcccggct ttgtctttgg tcagcgggct ttcactgatt ttcttttgca
    31141 aggtttcagg ttgcatttcc tgcggataga ttggcccgat aatgccgtag cggttgcggc
    31201 ttggcaccat atagaccggt ttcgcgcctg tcagcatcaa accttgttcg atggatttat
    31261 ggcagttacg gtcaacgacc acgacatcgt tatcggtcat gcaagcctgc atgatggtgc
    31321 ggttagagcc ggaagtaccg acgactaccg accaggagcg atcggcacca aatacgcgtg
    31381 cggcatattt ttcgctttcg ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag
    31441 ttcgttcgat gcccatgtcg gtgcggaaca gattttcacc atagtagtca tggtagaaac
    31501 gtccggcggg tgttttggta aaaccaacgc cgccctggtg gcctggcgct gcccaggaat
    31561 attcatggat gtcactatat ttcatcagcg cgctgaacag tggcggcaac agctgctggc
    31621 ggtagcgggt catcgcggca acggcgcgtc cggcgataaa gtcggcggta tcttccagaa
    31681 tccaggcgaa ttcatcgaca agctccagca gatcccggtc cattgcggcg agggcttttt
    31741 cccgatcgcc caacaggaag accggcacgt tttgttggcg ctcatgaagc ttaccgatca
    31801 attgtctgac gttttgatgt tcgtccggat gttccatttg atagctgaac atcaggcagt
    31861 caatggcttc gtttgaagag agaatggcaa aaccatcatc aaaggaggtg gatttaatca
    31921 cggtaacatt ttgctggctt aaagcatctg ccagacgctc aacggcgtta ccgacccagg
    31981 tgtcttgatg gagaaactcg ctttcaacaa ttaatacttt catcattgct tacccggtta
    32041 tgaaggaatc ttcgttgcgg gcaagtatct tccggccttt tcgggctgta aagcgcgtga
    32101 aaaacaaaca tttttgaaat atttagtttt ataaacaata cgttatgtga ttattttaaa
    32161 ttattttcac tgcttattat cgacggctaa actatttttt tggctgatgc tgatatcgtc
    32221 tttagccggg aaacgtctgg cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca
    32281 tgccgacata tttgccgaac gtgctgtaaa aacgactact tgaacgaaag cctgccgtca
    32341 gggcaatatc gagaatactt ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc
    32401 gcatcgcggt aatgtactgt ttcatcgtca attgcatgac ccgctggaat atccccattg
    32461 catagttggc gttaagtttg acgtgctcag ccacatcgtt gatggtcagc gcctgatcat
    32521 agttttcggc aataaagccc agcatctggc taacataaaa ttgcgcatgg cgcgagacgc
    32581 tgtttttgtg tgtgcgcgag gttttattga ccagaatcgg ttcccagcta gagaggctaa
    32641 atcgcttgag catcaggcca atttcatcaa tggcgagctg gcgaatttgc tcattcggac
    32701 tgtttaattc ctgctgccag cggcgtactt caaacgggct aagttgctgt gtggcaagtg
    32761 atttgatcac catgccgtga gtgacgtggt taatcaggtc tttatccagc ggccaggaga
    32821 gaaacagatg catcggcaga ttaaaaatcg ccatgctctg acaggttccg gtatctgtta
    32881 gttggtgcgg tgtacaggcc cagaacagcg tgatatgacc ctgattgata ttcacttttt
    32941 cattgttgat caggtattcc acatcgccat cgaaaggcac attcacttcg acctgaccat
    33001 gccagtggct ggtgggcatg atatgcggtg cgcgaaactc aatctccatc cgctggtatt
    33061 ccgaatacag cgacagcggg ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat
    33121 ctgtattcat ggatggctct ctttcctaga atatcagaat tatggcagga gtgagggagg
    33181 atgactgcga gtgggatcac ggttttcacc ctcttcccag aggggcgagg ggactgtccg
    33241 agcatcatga ggccgaaaac tctacttttc aggtaattta ttcccataaa ctcagattta
    33301 ctgctgcttc acgcaggatc tgagtttatg ggaatgctca acctggaagc cggaggtttt
    33361 ctgcagattc gcctgccatg atgaagttat tcaagcaagc caggagatct gcatgatgtc
    33421 tgcacccaaa attacattta tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg
    33481 tgatgtgttc catcgcgagg cgctgaaaac ggcgcatatt gccctgatgg acattgaccc
    33541 cacccgcctg gaagagtcgc atattgtggt gcgtaagctg atggattcag caggggccag
    33601 cggcaaaatc acctgccaca cccaacagaa agaagcctta gaggatgccg attttgtcgt
    33661 ggtggcattt cagattggcg gttatgaacc ttgcacggtg actgatttcg aggtctgtaa
    33721 gcggcatggt ctggaacaaa ccattgccga tacgttgggg ccgggcggta ttatgcgcgc
    33781 gctacgtacc attccgcatc tgtggcaaat ttgcgagaac atgacggaag tctgccccga
    33841 tgccaccatg ctcaactatg ttaacccaat ggcgatgaat acctgggcga tgtatgcccg
    33901 ctatccgtat atcaaacagg tcgggctgtg ccattcggtg cagggaacgg cggaagagct
    33961 ggcgcgtgac ctcaatatcg acccggctac gctgcgttac cgttgcgcag gtatcaacca
    34021 tatggcgttt tacctggagc tggagcgcaa aaccgccgac ggcagttacg tgaatctcta
    34081 cccggaactg ctggcggctt atgaagcagg gcaggcaccg aagccaaata ttcatggtaa
    34141 tactcgctgc cagaatattg tgcgctacga aatgttcaaa aagctgggct acttcgtcac
    34201 ggaatcgtca gaacattttg ccgaatatac gccgtggttt attaagctag gtcgtgagga
    34261 tttgattgag cgttataaag taccgctgga tgagtacccg aaacgctgcg tcgagcagct
    34321 ggcgaactgg cataaagagc tggaggagta taaaaacgcc tcccggattg atattaaacc
    34381 gtcacgggaa tatgccagca caatcatgaa cgctatctgg actggcgagc cgagtgtgat
    34441 ttacggcaac gtccgtaacg atggtttgat tgataacctg ccacaaggat gttgcgtgga
    34501 agtagcctgt ctggttgatg ctaatggcat tcagccgacc aaagtcggta cgctaccttc
    34561 gcatctggcc gccctgatgc aaaccaacat caacgtacag acgctgctga ccgaagctat
    34621 tcttacggaa aatcgcgacc gtgtttacca cgccgcgatg atggacccgc atactgccgc
    34681 cgtgctgggc attgacgaaa tatatgctct tgttgacgac ctgattgccg cccacggcga
    34741 ctggctgcca ggctggttgc accgttaaaa cgcgactaaa cgctactgcg ccgggggatt
    34801 tattccggcg cacacctctg acgataccaa taacagaagg cgggcgttgg taacagcgac
    34861 ccgataccct atgagcattt caatgactac aaaactcagt tatggatttg gagcgttcgg
    34921 gaaggatttt gcgatcggca ttgtgtatat gtacctcatg tattactaca ccgatgtcgt
    34981 cgggctgtct gtgggtttgg tcggtacttt gtttctggtg gcgaggatct gggatgctat
    35041 taacgatccg attatgggat ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa
    35101 actctggatc ctgatcggta cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc
    35161 gcatctgttt gaaggtacta ctcagattgt ctttgtttgc gtgacctaca tcctctgggg
    35221 catgacttac accattatgg atattccctt ctggtcgctg gttccaacca tcacgctcga
    35281 taaacgtgaa cgcgaacaac tggttcctta tccgcgtttt tttgccagtc tggcaggctt
    35341 tgttacggca ggtgtgacgc taccatttgt taattatgtc ggcggtggcg atcggggatt
    35401 tggctttcag atgttcactc tggtattgat cgcctttttt attgtttcaa ccatcatcac
    35461 tctgcgcaat gtgcatgaag tcttttcgtc agacaatcaa tcgtctgctg aaggaagcca
    35521 tctgacactt aaagccatcg ttgcgcttat ttataaaaac gatcagcttt catgcctctt
    35581 gggtatggct cttgcttata atgtagccag caacattatt accggatttg ctatctatta
    35641 tttctcatat gttatcggtg atgcggattt gttcccctat tatctgtcgt atgcgggagc
    35701 tgctaacctg gtgacgttag tattcttccc acgcttagtt aaatcattat cccgacgcat
    35761 tttatgggcc ggagcatcta ttcttccggt gttaagctgt ggtgttctcc tgttaatggc
    35821 attaatgagc tatcacaacg tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg
    35881 aacggcgctt ttctgggtat tacaggtcat catggtggca gataccgttg attacggtga
    35941 atataaactg cacgtacgct gtgaaagcat cgcttactcc gtgcagacta tggtggtgaa
    36001 gggcggttca gcctttgcgg cttttttcat tgcggttgtg ttagggatga ttggctatgt
    36061 accgaatgtt gaacagtcta cgcaagccct attaggtatg cagtttatta tgattgctct
    36121 accgactctg tttttcatgg taacgctgat tctctacttc cgtttctatc gcctcaatgg
    36181 cgacacgctg cgcaggatcc agattcatct gctggataaa tatcgcaaag taccgcccga
    36241 gcctgttcat gctgatattc cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca
    36301 atagctaacg aaaatggcct gatgcgatat gtttatcagg ccacgtctta atgtaatgct
    36361 ttggatttgc aaattttgta gaccggataa ggaattcacg ccgcatccgg catcaacaaa
    36421 gtgcacgttg ttatccggtt atcaagccat agcgccgtag ctggcggcaa tgaaagcgag
    36481 cgagcaatat cactgtcgtt aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt
    36541 cccactggtc gccctgggta ccgagaaaat catccgctcc ctggcccatc gccagtgccg
    36601 cccaccactc gattaattca tacatcgcgc ttatcgccag cgccacgcaa cagaccagaa
    36661 acgccaccat tttacgtccg cgcacgtaca tcccgcgaac gagaatttct cgtgccacca
    36721 gtgcaggcac cagcccctgg aaaaagtgcc ccagcttgtc atacggatta cgactcaacc
    36781 ccagccattc ctgcacctca aaaccaacgg ggactttcgc gtaggtgtac tgcccgccta
    36841 ccatcaggat gatggcgtga aagaagatga gcgtatagag cagcagcgtt aacgggtaac
    36901 gtctggcggt ggcaagtagt agcggcacga cgataatcac cggtgtcact tccagcagcc
    36961 aggtgagttt gtcatgggcc gaaatgccgg tataaatcag gattaacgtt agcgccagtg
    37021 cgccggtgtt aagaattaac ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg
    37081 aaaatactga ctattcacca gcaagcatga aattacaatc gcaaaagagg tggcccaggg
    37141 ggatcacctg gcagcatgct gccaggcgct gggccgaaga ggttacttag tgcagttcgc
    37201 gcactgtttg ttgacgattt gctggaagaa gtcgttacct ttgtcatcga ccaggataaa
    37261 cgccgggaaa tcttctactt cgattttcca gatagcttcc atacccagct ccggataagc
    37321 gacgcattcc agatgcttga tgctctgctg cgccagtacc gccgccggac cgccgatgct
    37381 accgaggtag aagccgccgt gtttatgaca cgcgtcggta acctgctggc tgcggttgcc
    37441 tttcgccagc atgatcatgc tgccgccgtg ggattgcagc agatccacat aggagtccat
    37501 acgacctgcg gtggttgggc caagtgaacc tgatggataa ccggcagggg ttttcgccgg
    37561 acccgcgtag tagatcgggt gatctttgat gtactgcgga agttctttac cggcgtcaat
    37621 cagctctttc agcttggcgt gtgcaatatc tcgacccaca ataatggtgc cagtgagcga
    37681 caaacgagtg gaaaccggat attgcgaaag ctgggcgagg atctctttca tcgggcggtt
    37741 aaggtcgacc ttcaccgctt cgccttcacc ggcctgacgc agttcttgtg gaatgtactg
    37801 acctagattg tgttccagtt tttcgatcca gataccttcg cggttgattt tcgctttaat
    37861 gttacggtca gcggagcagg agacgcccat gcctaccggg caggatgcgc cgtgacgtgg
    37921 cagacggata acgcgaatgt cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc
    37981 gagtttctgg gcctcttcca gcagttcctg ttccagctgg acatcgcgga acgcctgacc
    38041 atgttcgttc ccttccgtcg gcagttcatc gtaatagtga gcgcttgcta atttgacggt
    38101 tttcaggttg gtttccgcag acgtaccgcc aatcacaaac gcgatatggt acggcgggca
    38161 ggctgcagta ccgagggtac gcattttctc gacgaggaag tttttcagtt tgccgggagt
    38221 cagcagggct ttggtttcct ggtagagata cgttttgttg gcagagccgc cgcctttcgc
    38281 aacgcaaagg aatttgtact catcgccatc taccgcgtac aggtcgattt gcgcaggcag
    38341 gttagtgccg gtgttgacct ctttgtacat gtccagcgcc gcgttttgcg agtagcgcag
    38401 gttatcttcg atataggtgt tatagacgcc tttcgacagc gcttcttcat caccgccacc
    38461 ggtccacacg cgctggcctt ttttaccgac gatgatcgcg gtgccggtat cctggcaggt
    38521 cggcagcacg cctttggcgg cgatttcgga gtttcttaag aattgcagtg ccacgtactt
    38581 gtcgttttcg ctggcttctg gatcgtgaag aatagccgca acctgtttct ggtgtgccgg
    38641 acggagcatg aaagaagcgt cgtgaaaggc ttgctgcgcc agcagggtca gggcttctgg
    38701 ttccactttc aggatggttt cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag
    38761 tagatagtat tcggtattgt ctttccccat cgggaaaggt gcttggtaga taaagggttt
    38821 gtttgacata gcttccagcc tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt
    38881 agtcacgggc ggcacgcaaa gtgcatttat aagaacccgt acatcgcggc gaagatccag
    38941 ccgaagacgc acgatacgct cacaccaatc aaccccggca gaataaaact gtggttgatg
    39001 acgaagcgac cgatgtgggt ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc
    39061 ggataagtcg gcaggatgta ataaccgtag caagccggtg ctgaagccac gatgtatgcc
    39121 ggatcaacgc cgatcgccag cgcgaccgga acaatcgccg ccagcgccgc agcctgagag
    39181 tttacaaact tggaaaccag cagcagaaca atggcatacg cccacggata ctctttcacc
    39241 atttcaccca gtacgccctg aatttcagac atatgcgcac cgaacatggt ttctgccatc
    39301 catgcgatac cgtacaccgc cacgatggcg atcataccgg aacggaagac ttcgtttttt
    39361 gagatagacg cgggattggt tttggtcagg ataataatca gcgccccggt cagcagcata
    39421 aacatctgaa taaccagtac catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc
    39481 gaatcagcac caagaagggc gactacagcg attgccccga ggaaaatcca cattgccagc
    39541 cagttgcttt tcggcagttt tttatccagc agcgtcgcgg tatcaccgta aacatactca
    39601 cggttttccg gtacggagat gaatttctgg aactcttcgt ctttatccag atctttaccg
    39661 cggaaccagc tgaagatacc gatcgccagg ataccgatta acgtcgatgg aatggtgatt
    39721 gccagcagat cgaggaactc aagatggcga ccatcaaagg tgacattacc cagcatcgca
    39781 accagagaca cgaccgcaac cgacaccgga ctggcgataa tccccatctg tgcaccgata
    39841 gaacttgccg ccatcggacg ttccggacgg atgttgttct taatggcgac gtcgtagatg
    39901 atcggcagaa tggtgtaaac cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc
    39961 acaaacggcg cgacaattga gacatatttc gggttgcggc gcagcagctt ctcggcaatt
    40021 tgcagcatga catcaagacc gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca
    40081 atgataacca gcatgacatc aactggtggt ttacctggct gaaggtggaa gacgaagacc
    40141 agaatgacca gaccgatacc gcctaataaa cccagcgcga taccaccctt tctggcacca
    40201 taaaacagac atatcagtat tatgataagt tggatagtaa ataacatgtg tgaaccctcg
    40261 cgataatcct atttaaattt ttgctgaata gatcacagtc acgttctgtt ttgtatgaac
    40321 tgttttcaga gcctgactaa atatccgttt ggtcgttacc ggctttagca aatacctcac
    40381 agtgaatatt ggctgattaa agcagcggca aatttatgca ctgtgataag cggctttttc
    40441 agagagaaaa actctctggc gatgggttta tagtttgtac tgcgcagatg ttaatgtttt
    40501 gtttgctgtt atcttattga tatttatcgg tctatttttt gttctcaggg cagatatcat
    40561 taggtttata gattaatctg atctacccat ttgtgggtaa aaatacacat aatgcgggtg
    40621 acataatagt taattaactt ttgttatcgt tttgaaatta aaaacaccgt tcatctgaag
    40681 agatattaat ttttaacgat gatggaggga taattatatt tgatctggca caagttttac
    40741 tgattaacga tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac
    40801 agataaccag cgatccgtta ttggcaatat tgtttcagca gtgagtagtg ttctgcctga
    40861 atacggtaac ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaagatg
    40921 tggcagttga ccagccagat gaggtatttt cggcaggaaa cacgcgagat gttaacctcg
    40981 ttagccagct cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc
    41041 gtgcgtaacg tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ttcgttggag
    41101 ctgctgccgt ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc
    41161 gccattttct tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt
    41221 ttgatcaggt aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct
    41281 gcggaggaga tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa
    41341 tcgagcccgt tctctttttg catatagata tcgagcaata tcaggtcgat aggcgcatcg
    41401 ctattgaaga taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag
    41461 cctgggattt gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata
    41521 attaatacat tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt
    41581 gtgtgaaaat ccccggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt
    41641 gtttgacaag tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa
    41701 aaatgtgatc gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca
    41761 gccagccgtg acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg
    41821 cttccagcgc gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct
    41881 cactgccgct gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg
    41941 tcgcgcggtt aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca
    42001 gcagagagcc aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact
    42061 gcttataact cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt
    42121 gggatcgttc acgaagtgcg tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt
    42181 tacgaacttc agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc
    42241 gcaccggaac ggtgttgatc agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc
    42301 gcggcgtacc gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga
    42361 gcgtcgacag tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat
    42421 cgttgatcag cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact
    42481 gcaacatggc ctggcgttgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa
    42541 ggattttttt cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa
    42601 ataataccga ccagataatg ctccagcgac tgtcattgat ctgttgggtc acgcggctta
    42661 actcaaggcc gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa
    42721 atacgcgtaa agcctgcgcc agaaaaccgc gattgatagc gacattttct tcgccattca
    42781 gcgcattaag gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgtg
    42841 agtagcgaag actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca
    42901 cggcttccgc gatggcctgg atgccactct cctgcggttt tttctgcaag ccctgacgga
    42961 tttccggcga gtcggcgagg gtacgcgcca ctgccagtgc cttgttggcc agcccgtctc
    43021 gcgtcatatc actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca
    43081 gtaccgcgct gaccattaag atcacagtgg tactcaattt catcggacgt ttgcgtaata
    43141 tgtggtaggg caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat
    43201 ctctgatgag gcgggtaatt caaagggagt aaaaatgatt ggctatatag gggaagagac
    43261 tctggcaacg gaaactgcca gtgctgtatg aagattccgg ggctatgctt atagcgataa
    43321 tcatactgat gagagaggga aggtcatgga tcaggcgcta ctggacgggg gttatcgctg
    43381 ttataccggc gaaaagatcg atgtctattt caacactgcg atatgtcagc attctggcaa
    43441 ttgcgtacgt ggcaacggca agttatttaa tctcaaacga aagtcg
//
LOCUS       DADLSE010000018        42007 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLSE010000018 DADLSE010000000
VERSION     DADLSE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42007
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.018"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            121..2655
                     /gene="mrcB"
                     /locus_tag="JN692_000990"
     CDS             121..2655
                     /gene="mrcB"
                     /locus_tag="JN692_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7263364.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2875..5118
                     /gene="fhuA"
                     /locus_tag="JN692_000991"
     CDS             2875..5118
                     /gene="fhuA"
                     /locus_tag="JN692_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7263365.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKNGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5169..5966
                     /gene="fhuC"
                     /locus_tag="JN692_000992"
     CDS             5169..5966
                     /gene="fhuC"
                     /locus_tag="JN692_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7263366.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5966..6856
                     /gene="fhuD"
                     /locus_tag="JN692_000993"
     CDS             5966..6856
                     /gene="fhuD"
                     /locus_tag="JN692_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7263367.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6853..8835
                     /gene="fhuB"
                     /locus_tag="JN692_000994"
     CDS             6853..8835
                     /gene="fhuB"
                     /locus_tag="JN692_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7263368.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="JN692_000995"
     CDS             complement(8870..10150)
                     /gene="hemL"
                     /locus_tag="JN692_000995"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7263369.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEESGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10375..11796
                     /gene="clcA"
                     /locus_tag="JN692_000996"
     CDS             10375..11796
                     /gene="clcA"
                     /locus_tag="JN692_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7263370.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11820..11885
                     /locus_tag="JN692_000997"
     CDS             11820..11885
                     /locus_tag="JN692_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263371.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            11878..12222
                     /gene="erpA"
                     /locus_tag="JN692_000998"
     CDS             11878..12222
                     /gene="erpA"
                     /locus_tag="JN692_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7263372.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12269..12892)
                     /locus_tag="JN692_000999"
     CDS             complement(12269..12892)
                     /locus_tag="JN692_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7263373.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="JN692_001000"
     CDS             complement(12930..13730)
                     /gene="btuF"
                     /locus_tag="JN692_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7263374.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="JN692_001001"
     CDS             complement(13723..14421)
                     /gene="mtnN"
                     /locus_tag="JN692_001001"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7263375.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGCLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNIPFVVVRAISDVADQQSHLSFDESLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14505..16022
                     /gene="dgt"
                     /locus_tag="JN692_001002"
     CDS             14505..16022
                     /gene="dgt"
                     /locus_tag="JN692_001002"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7263376.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRINLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16152..17576
                     /gene="degP"
                     /locus_tag="JN692_001003"
     CDS             16152..17576
                     /gene="degP"
                     /locus_tag="JN692_001003"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7263377.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17731..18887
                     /locus_tag="JN692_001004"
                     /pseudo
     CDS             17731..18887
                     /locus_tag="JN692_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(18976..19362)
                     /locus_tag="JN692_001005"
     CDS             complement(18976..19362)
                     /locus_tag="JN692_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7263378.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19676..20500)
                     /gene="dapD"
                     /locus_tag="JN692_001006"
     CDS             complement(19676..20500)
                     /gene="dapD"
                     /locus_tag="JN692_001006"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7263379.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20531..23203)
                     /gene="glnD"
                     /locus_tag="JN692_001007"
     CDS             complement(20531..23203)
                     /gene="glnD"
                     /locus_tag="JN692_001007"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7263380.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23265..24059)
                     /gene="map"
                     /locus_tag="JN692_001008"
     CDS             complement(23265..24059)
                     /gene="map"
                     /locus_tag="JN692_001008"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7263381.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24427..25152
                     /gene="rpsB"
                     /locus_tag="JN692_001009"
     CDS             24427..25152
                     /gene="rpsB"
                     /locus_tag="JN692_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7263382.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25410..26261
                     /gene="tsf"
                     /locus_tag="JN692_001010"
     CDS             25410..26261
                     /gene="tsf"
                     /locus_tag="JN692_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7263383.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26408..27133
                     /gene="pyrH"
                     /locus_tag="JN692_001011"
     CDS             26408..27133
                     /gene="pyrH"
                     /locus_tag="JN692_001011"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7263384.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27425..27982
                     /gene="frr"
                     /locus_tag="JN692_001012"
     CDS             27425..27982
                     /gene="frr"
                     /locus_tag="JN692_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7263385.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28074..29270
                     /gene="ispC"
                     /locus_tag="JN692_001013"
     CDS             28074..29270
                     /gene="ispC"
                     /locus_tag="JN692_001013"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7263386.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29459..30217
                     /gene="ispU"
                     /locus_tag="JN692_001014"
                     /gene_synonym="uppS"
     CDS             29459..30217
                     /gene="ispU"
                     /locus_tag="JN692_001014"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7263387.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30230..31087
                     /gene="cdsA"
                     /locus_tag="JN692_001015"
     CDS             30230..31087
                     /gene="cdsA"
                     /locus_tag="JN692_001015"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7263388.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31099..32451
                     /gene="rseP"
                     /locus_tag="JN692_001016"
     CDS             31099..32451
                     /gene="rseP"
                     /locus_tag="JN692_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7263389.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMFVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32481..34913
                     /gene="bamA"
                     /locus_tag="JN692_001017"
     CDS             32481..34913
                     /gene="bamA"
                     /locus_tag="JN692_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7263390.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35035..35520
                     /gene="skp"
                     /locus_tag="JN692_001018"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35035..35520
                     /gene="skp"
                     /locus_tag="JN692_001018"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7263391.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35524..36549
                     /gene="lpxD"
                     /locus_tag="JN692_001019"
     CDS             35524..36549
                     /gene="lpxD"
                     /locus_tag="JN692_001019"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7263392.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36654..37109
                     /gene="fabZ"
                     /locus_tag="JN692_001020"
     CDS             36654..37109
                     /gene="fabZ"
                     /locus_tag="JN692_001020"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7263393.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37113..37901
                     /gene="lpxA"
                     /locus_tag="JN692_001021"
     CDS             37113..37901
                     /gene="lpxA"
                     /locus_tag="JN692_001021"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7263394.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37901..39049
                     /gene="lpxB"
                     /locus_tag="JN692_001022"
     CDS             37901..39049
                     /gene="lpxB"
                     /locus_tag="JN692_001022"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7263395.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39046..39642
                     /gene="rnhB"
                     /locus_tag="JN692_001023"
     CDS             39046..39642
                     /gene="rnhB"
                     /locus_tag="JN692_001023"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7263396.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            39679..>42007
                     /gene="dnaE"
                     /locus_tag="JN692_001024"
     CDS             39679..>42007
                     /gene="dnaE"
                     /locus_tag="JN692_001024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7263397.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLTFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKA"
BASE COUNT         9872 a        10289 c        11665 g        10181 t
ORIGIN      
        1 ccctctccct caagggagag gggaccgatc gagcacaaat tttgagagat atcttcttct
       61 gtcttgtaac agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc
      121 atggccggga atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa
      181 caaaaggtaa gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat
      241 gaggatgaag aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt
      301 ggcaaacgcg gctggctatg gctactgcta aaactggcta tcgtttttgc cgtgctgatc
      361 gccatttacg gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg
      421 caactgcccg cggcagttta tggccgaatg gtcaaccttg aaccagatat gaccatcagc
      481 aagaacgaaa tggtgaagct gctggaggca acacagtatc gtcaggtgtc gaaaatgacc
      541 cgtcctggcg aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat
      601 ttcccggaca gtaaagaagg gcaggtgcgt gcgcgtctga cctttgatgg tgatcatctg
      661 gcgaccatcg tcaatatgga gaacaaccgt cagttcggtt tcttccgtct tgatccgcgt
      721 ctgatcacca tgatctcctc gccaaacggt gagcagcgtt tgtttgtgcc gcgcagtggt
      781 ttccctgatt tgctggtgga tactttgctg gcgacagaag accgtcattt ttacgagcat
      841 gatggaatca gtctctactc cattgggcgt gcggtgctgg caaacctgac cgcaggtcgc
      901 acggtgcagg gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc
      961 gagcgttctt actggcgtaa agcgaacgaa gcttacatgg cgctgatcat ggacgcgcgt
     1021 tacagcaaag accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc
     1081 gacaacgaaa tccgcggctt cccgctggca agcttgtatt actttggccg cccggtagaa
     1141 gagctaagcc tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac
     1201 aacccgtggc gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtctgctg
     1261 caacagcaac agattattga tcaagaactc tatgacatgt tgagtgcccg tccgctgggg
     1321 gttcagccgc gtggtggggt gatctctcct cagccagcct ttatgcagct ggtgcgtcag
     1381 gagttgcagg caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact
     1441 acctttgact cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca
     1501 ctgaagaaac agcgtaagtt gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt
     1561 agtggtgaag ttcgtgcgat ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt
     1621 gcgatgcagg cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct
     1681 ttaagccagc cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg
     1741 cgtcagccga atggccaggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc
     1801 ggcagagtga tgctggtgga tgcgttgaca cgttcgatga acgtgccgac ggtaaatctg
     1861 gggatggcgc tggggctgcc tgcggttacg gagacctgga tcaaactggg cgtaccgaaa
     1921 gatcagttgc atccggttcc ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa
     1981 gtggcgcagg cattccagac catcgccagc ggtggtaacc gtgcaccgct ttctgcactg
     2041 cgttcggtaa tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc
     2101 gctgttccgg cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt
     2161 ggtacgggtc gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg
     2221 actaccaaca ataacgtaga tacctggttt gcaggcattg acggcagcac ggtgaccatc
     2281 acctgggtag gccgtgataa caaccagccg accaaactgt atggagccag cggggcaatg
     2341 tcgatttatc agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca
     2401 gaagatattg cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg
     2461 cgtgtcttgc cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag
     2521 cagcagccgt caggcaatcc gtttgatcag tcttctcagc cgcagcaaca gccgcaacag
     2581 caacctgctc agcaagagca gaaagacagc gacggtgtag ccggttggat caaggatatg
     2641 tttggtagta attaacatct aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa
     2701 taacatccca tctaagatat taaccctttc ttttcatctg gttgtttatt aacccttcag
     2761 gaacgctcag attgcgtacc gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt
     2821 tataataatc attctcgttt acgttatcat tcactttaca tcagagatat accaatggcg
     2881 cgttccaaaa ctgctcagcc aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca
     2941 gcggttagcg gcatgtctgt ttatgcacag gcagcggttg aaccgaaaga agacactatc
     3001 accgttaccg ctgcacctgc gccgcaagaa agcgcatggg ggcctgctgc aactattgcg
     3061 gcgcgacagt cagctaccgg cactaaaacc gatacgccga ttcaaaaagt gccacagtct
     3121 atttctgttg tgaccgccga agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg
     3181 cttagctaca cgccgggtgt ctctgttggt acgcgtggcg catctaacac ctatgaccac
     3241 ctgatcattc gcggttttgc ggcagaaggc caaagccaaa ataactatct gaatggcctg
     3301 aagttgcagg gcaacttcta taacgatgcg gtcattgacc cgtatatgct ggaacgcgct
     3361 gaaattatgc gtggcccggt ttccgtgctt tacggtaaaa gcagtcctgg cggtctgttg
     3421 aatatggtca gcaagcgtcc gaccaccgaa ccgctgaaag aagttcagtt taaagccggt
     3481 actgacagcc tgttccagac tggttttgac tttagcgatg cgttggatga tgacggtgtt
     3541 tactcttatc gcctgaccgg tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa
     3601 gagcagcgtt atgctattgc accggcgttc acctggcgtc cggatgataa aaccaatttc
     3661 accttccttt cttacttcca gaacgagccg gaaaccggtt attacggctg gttgccgaaa
     3721 gagggaaccg ttgagccgct gccgaacggt aagcgtctgc cgacagactt taatgaaggg
     3781 gcgaagaaca acacctattc tcgtaatgag aagatggtcg gctacagctt cgatcacgaa
     3841 tttaacgaca cctttactgt gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa
     3901 aacagcgttt atggttacgg cgtctgctcc gatccggcga atgcttacag caaacagtgt
     3961 gcggcattag cgccagcgga taaaggccat tatctggcac gtaaatacgt cgttgatgat
     4021 gagaagctgc aaaacttctc cgttgatacc cagttgcaga gcaagtttgc cactggcgat
     4081 atcgaccaca ccctgctgac cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc
     4141 tggtttggtt acgacgactc tgtgccactg ctcaatctgt acaatccggt gaataccgat
     4201 ttcgacttca atgccaaaga tccggcaaac tccggccctt accgcattct gaataaacag
     4261 aaacaaacgg gcgtttatgt tcaggatcag gcgcagtggg ataaagtgct ggtcacccta
     4321 ggcggtcgtt atgactgggc agatcaagaa tctcttaacc gcgttgctgg gacgaccgat
     4381 aaacgtgatg acaaacagtt tacctggcgt ggtggtgtta actacctgtt tgataatggt
     4441 gtaacacctt acttcagcta tagcgaatcg tttgaacctt cttcgcaagt tgggaagaat
     4501 ggtaatattt tcgcaccgtc taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg
     4561 gaagatcgtc cgattgtagt tactggtgcc gtgtataatc tcactaaaac caacaacctg
     4621 atggcggacc ctgagggttc cttcttctcg gttgaaggtg gcgagatccg cgcacgtggc
     4681 gtagaaatcg aagcgaaagc ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact
     4741 tacaccgatg cggaatacac caccgatact acctataaag gcaatacgcc tgcacaggtg
     4801 ccaaaacaca tggcttcgct gtgggctgac tataccttct ttgacggtcc gctttcaggt
     4861 ctgacgctgg gcaccggtgg tcgttatact ggctccagct atggtgatcc ggctaactcc
     4921 tttaaagtgg gaagttatac ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc
     4981 ggcatggcgg gctccaacgt ggcgctgcat gttaacaacc tgttcgatcg tgaatacgtc
     5041 gccagctgct ttaacactta tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc
     5101 gcaacctttc gtttctaatt tctcttttgg ggcacggatt tccgtgccca tttcacaagt
     5161 tggctgttat gcaggaatac acgaatcatt ccgataccac ttttgcactg cgtaatatct
     5221 cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc gttaaccttt cctgccggga
     5281 aagtgaccgg tctgattggt cacaacggtt ctggtaaatc cactctgctc aaaatgcttg
     5341 gtcgtcatca gcctccgtca gaaggggaga ttcttcttga tgcccagccg ctggaaagct
     5401 ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc gcagcagctt cctccggcag
     5461 aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg
     5521 ggcgctttgg tgcggcagat cgcgaaaagg tcgaggaagc tatctcgctg gttggcttaa
     5581 aaccgctggc gcatcggctg gtcgatagtc tctctggcgg cgaacgtcag cgggcgtgga
     5641 tcgccatgct ggtggcgcag gatagccgtt gtctgttgct cgacgaaccg acttcggcgc
     5701 tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca ccgtttaagt caggaacgtg
     5761 gcctgacggt cattgccgtg ttgcacgata tcaatatggc ggcacgctac tgtgattatc
     5821 tggtcgccct gcgcagtggt gaaatgattg ctcagggaac gcctgcggaa attatgcgcg
     5881 gcgaaaccct cgaaatgatt tatggcatcc cgatgggtat tttgccgcat ccggcgggtg
     5941 ctgcacctgt gagttttgtt tattgatgag cggcttacct cttatttcgc gccgtcgact
     6001 gttaacggcg atggcgcttt ctccgttgtt atggcagatg aataccgccc acgcggcggc
     6061 tattgacccc aatcgtattg tggcgctgga gtggttgccg gtggaattac tgctggcgct
     6121 cggcatcgtg ccttacggcg tggcggatac catcaactat cgcctgtggg tcagcgaacc
     6181 accattgccg gactcagtga ttgacgtcgg tttgcgtaca gaacctaacc ttgaactgct
     6241 gaccgaaatg aaaccgtcgt ttatggtctg gtcggcagga tatggccctt catcagaaat
     6301 gctggcacgt attgcgccgg gtcgcggatt taacttcagt gacggcaaac atccgttggc
     6361 gatggcgcgt aaatcgctga cggaaatggc agatttactt aacctgcaaa gcgcagcgga
     6421 aacgcattta gcgcaatacg aagactttat ccgcagcatg aaaccccgct ttgtgaagcg
     6481 tggtgcacgc ccgttactgc tgacgacgct tatcgatccg cgccatatgc tggtcttcgg
     6541 tccaaacagc ttgttccagg aaattctcga tgagtacggc atcccaaatg cctggcaagg
     6601 ggaaaccaac ttctggggca gtaccgccgt cagtatcgat cgtctggcag cgtataaaga
     6661 cgttgatgtg ctctgttttg atcacgacaa cagcaaagac atggatgcgc taatggcaac
     6721 cccgctgtgg caggccatgc cgtttgtccg tacagggcgc tttcagcgcg tacccgcagt
     6781 ctggttttat ggtgcaacgc tttcggcaat gcactttgtg cgtgttctgg ataacgccat
     6841 cggaggtaaa gcgtgagtaa acgaattgcg cttttcccgg cgttattgct ggcgctgtta
     6901 gtgattgtcg ctacggcgct cacctggatg aacttctcac aggcgctgcc gcgcagtcag
     6961 tgggcgcagg ccgcctggtc gccggatatt gacgtcatcg agcagatgat ttttcactac
     7021 agcttgttgc cgcgtctggc gatttcgctg ctggtgggcg cgggcctggg gctggtgggc
     7081 gtgctgtttc agcaagtact gcgtaacccg ctggcggaac cgacgacgtt gggcgttgca
     7141 acaggcgcgc aactggggat taccgtcacc acgctctggg cgatccctag cgcgatggcg
     7201 agccagtttg ctgcgctggc aggggcttgc gttgttggct taattgtctt tggcgtcgcg
     7261 tgggggaaac ggctgtcgcc ggtaacgctg attctcgcgg ggttggtagt gagcctttat
     7321 tgcggcgcaa tcaatcagtt actggttatc ttccatcatg accagttgca aagcatgttt
     7381 ctgtggagca ctggaacgct gacgcaaacc gactggggcg gtgttgagcg tttatggccg
     7441 cagttgctgg gcggtgtgat gctgacgttg ctgctacttc gtccgttaac cctgatgggg
     7501 cttgatgatg gcgtggcgcg caatctcggg ctggccttgt cgcttgcgcg tctggcggcg
     7561 ctgtcgctgg cgattgtcat cagtgcgctg ctggtgaacg ctgtggggat tatcggcttt
     7621 atcgggttgt tcgcgccgct gctggcaaaa atgctggggg cgcggcgtct gctgccacga
     7681 ctgatgctgg cgtcgttgat tggtgcgctg atcctctggc tttccgatca aatcatcctc
     7741 tggctgactc gcgtgtggat ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg
     7801 ccgctgctac tgtggctgct gccgcgttta cgcagcatta gcgcgccgga tatgaaggtt
     7861 aacgatcgtg tcgcgactga acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg
     7921 ctgttgttga tggctgtggt ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg
     7981 tgggcgagcg gggcgttgct cgaggattta atgccctggc gctggccgcg aattatggcg
     8041 gcgctgtttg cgggcgtcat gctggcggta gcgggctgta ttattcagcg actgaccggc
     8101 aacccgatgg caagcccgga agtgctgggg attagctccg gcgcggcgtt tggtgtggtg
     8161 ttaatgctgt ttctggtacc gggtaatgcc tttggctggc tgttacccgc aggcagtctc
     8221 ggggcggcgg tgacgctgtt gatcattatg atcgccgccg gacgcggtgg attttcccca
     8281 caccgcatgt tactggcggg gatggcgtta agtaccgcgt tcaccatgct tttgatgatg
     8341 ttgcaggcaa gtggtgaccc gcgaatggcg caagtgctga cctggatttc cggttctacc
     8401 tacaacgcga ccgatgctca ggtctggcgc accggaattg tgatggtgat tttgctggcg
     8461 attactccgc tgtgccgccg ctggctgacc attttaccgc tgggtggtga taccgcgcgg
     8521 gcggtaggga tggcgctgac gccgacgcga attgcgctgc tgctgttagc ggcttgcctg
     8581 acggcgaccg cgacgatgac tattggaccg ttgagttttg ttggtttaat ggcaccgcat
     8641 attgcgcgga tgatgggctt tcgacggacg atgccacaca tcgtaatttc ggcgctggtg
     8701 ggtggtttac tgctggtgtt cgctgactgg tgtgggcgga tggtgctgtt tccattccag
     8761 atcccggcgg ggctgctgtc gacctttatc ggtgcgccat attttatcta tttgctgaga
     8821 aagcagagcc gttaatagaa tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg
     8881 cgaacacccg acgtgcagca tcgatggtgt tattgatatc ttccatgctg tgcgccacag
     8941 acataaagcc cgcttcaaac gctgacggtg ccaggtaaac accttcatcc agcatcatat
     9001 ggaagaaacg cttaaaacgc tcgacgtcac aggccatcac atcctgatag cacgtcacgg
     9061 actcggcgtc ggtaaagaag ataccgaaca taccaccgac gtggttaacg accagcggaa
     9121 ttcccgattc ttctgccgct tccagcagac cttctgccag acgtgttgtc agctcatcca
     9181 gcgtttcgtg aatgcccggc tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg
     9241 caatcgggtt accggaaagc gtacccgcct gatagaccgg acctgtcggg gccagcgcat
     9301 ccattacatc acgacgacca ccgaatgcgc ctaccggcat tccaccgccg atgatttttc
     9361 cgaggcaggt taaatctggc actacgccga aataatcctg tgcgccagct agcgctacgc
     9421 ggaaaccggt catcacttca tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac
     9481 gcagacctgg caggaactct ggcagcggcg gaacacagtt catattgcct gccaccggct
     9541 cgacgataat acaggcaatc tcttgcgggt attgctcaaa cgcggcgcgt acagaggcca
     9601 gatcgttata agtacaggtt aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt
     9661 ttggctggcc taacgtgagt gcgccagaac cggctttcac cagcaggcag tcagcgtgac
     9721 cgtggtaaca accttcaaat ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc
     9781 ggatggcgct catcgtcgcc tcggtgccgg agttcaccat gcgcaccata tccatggtcg
     9841 ggaccagttc ggtcaccagt tgcgccattt tcacttccat ttcggtcggt gcaccaaagc
     9901 ttaaaccacg ctcggcggct tcaatcacgg cattgcggat cgccggatgg ttatggccca
     9961 gcaccatcgg cccccaggaa ccgacataat cgatataggc tttgccatca acatcgtaca
    10021 gataagcgcc gtccgctttt tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc
    10081 gaacagggga gttcacaccg ccagggatca gctcgcgcgc tgcgctgtaa agattttcag
    10141 acttactcat ggagggttcc tgattcgtag aaaaagtgaa tggctgctat tctatgttat
    10201 tcataacaag ttaaatactc gtcaaacatc aggctgcttg tactggtgca atcctgattt
    10261 cttagagtat aaaagttttg tgcatttgaa acattacgct ttgcaaagga ttttcatgga
    10321 acgtgcgagt aaaatgccgt catcttattt gtatgaccaa taagtgatca ttggatgaaa
    10381 actgatactc cctctttaga aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt
    10441 cgccaacttc ttgagcgcga taaaaccccg ttagccattt tgtttatggc ggcagtcgtc
    10501 ggcacgcttg tcgggctggc tgcggttgct tttgacaaag gtgtcgcctg gttgcagaac
    10561 caacgtatgg gggcgctggt acatactgct gataattatc cgctactgtt aaccgtcgct
    10621 tttctctgtt cggcggtgct ggcgatgttc ggctactttt tggtgcgcaa atacgcgccc
    10681 gaagcagggg gatcggggat cccggaaatt gaaggggcgc tggaagatca acgtcctgtt
    10741 cgctggtggc gtgtattgcc ggtgaaattc tttggcgggc tggggacact cggcggaggc
    10801 atggtgctag ggcgcgaagg accaaccgtg cagatcggcg gcaatatcgg tcgcatggtg
    10861 cttgatattt tccgcctgaa aggtgatgaa gctcgccata cgctgctggc aaccggtgct
    10921 gctgcggggc tggctgcggc ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa
    10981 gagatgcgtc cgcagtttcg ctatacgtta atttcgatta aagcggtatt tattggcgtc
    11041 attatgtcga ccattatgta ccggattttt aatcatgaag ttgcgttgat tgacgtcggt
    11101 aaactttctg atgcgccgct taatacgctg tggctttatc tgatcctcgg tattattttt
    11161 ggcattttcg gccctatttt taataaatgg gtgctgggga tgcaggattt gctgcaccgt
    11221 gtgcacggcg gcaatattac caaatgggta ctgatggggg gtgcgattgg cggtctgtgt
    11281 ggattgctgg ggtttgtggc accagcaacg tcgggcggcg gttttaacct gattcctatc
    11341 gctaccgcgg ggaatttcag catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt
    11401 accaccttac tctgcttctc ttccggcgcg ccgggcggta tttttgcccc gatgctggcg
    11461 ctgggtactg tgctgggaac cgctttcgga atggttgccg ttgagctgtt tccgcaatat
    11521 cacctggagg cggggacgtt tgctattgcc ggaatggggg cattactggc ggcatctatt
    11581 cgcgcgccgt taacggggat cattctggtt ctggagatga ccgataacta ccagctcatt
    11641 ttgccaatga ttattaccgg tcttggcgca acactattag cgcaatttac cggcgggaaa
    11701 cctctatact cggcgattct tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg
    11761 cgaagcaagg ccgcatcagc cagcgagaat acttgaacga aataccaggg tattagataa
    11821 tggcgattat tattgggtta gaatttgccc gattgccgat gtcgtttgga gcaaaatatg
    11881 agtgatgacg tagcactgcc gctggagttt accgacgcag cagccaacaa agttaaaagc
    11941 ctgatcgctg acgaagataa cccgaatctg aaattacgcg tgtatatcac cggtggcggt
    12001 tgcagcggct tccagtatgg tttcaccttt gatgatcagg tgaacgaagg cgatatgacc
    12061 atcgaaaaac agggcgttgg cctggtggtt gatccgatga gcctgcaata tctggtcggc
    12121 ggttccgttg actacaccga aggactggaa ggttctcgtt tcatcgtgac caacccgaac
    12181 gcgaaaagca cctgcggttg cggttcttcc tttagcatct aatctgttgt tcatgtgccg
    12241 gataaactat ccggcacatc ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg
    12301 gtagcttaag atgccaacga atagccgcca gccgaatcaa tagcgtcacg accatgccca
    12361 tcatactggc tgtttccagt ggtacggaaa atgtgtaata agccgtagcg tggacaatac
    12421 cgccgataat acaggcagtt gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg
    12481 ccagtacatc acgaatgatc ccgccgccaa cgccagtaat gacgcccata caaaccgcga
    12541 ttaacggacc ggcttccgca ttaaaggctt tattcacgcc aatgccgaca aacaccgcca
    12601 gaccaacggc gtccagcacc ggcaacatcc attttggtaa gcgtcttggc tggcgcacca
    12661 gcacgatggt cagcatgctg gtgaccatgg caacgaccag atcggtggga tctttcaccc
    12721 aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt tccgtcgcct actgcggtaa
    12781 ccacgcccag taccagaaca ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc
    12841 cggagatggc aaataccgct gtgccgacta tatccagcca atagacgagc attgttcaat
    12901 ccccactgag cacctgaaaa ggtcaggcac taatctacct gtgaaagcgc attacagagc
    12961 tgttgtgctg cgaggataat acgtgggctt gcacgttcaa accagtcact cgtgagagga
    13021 ataacgggaa ttttgagctg ttcaccccag tattgtttga ttttaggaat ttggtccggt
    13081 ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc
    13141 ggccagggaa cccggctgtc tttaaagatg ttttctccgc cacaaacttc gagtacctgg
    13201 ttctgaatcg actcttttcc actggtaaat ggcggattaa tgccgaattg cagaaaaaca
    13261 cgttttttag gtttatcagc atattgcgct ttcaattgca cgtactgatc cagcagggat
    13321 tgcgcggctt gttcggcctt gtctggttgc ggactccagg gggccagttg acgtaacgca
    13381 ttggcaattt gttcaatgct tgtcgcatcg acccacatca cttttattcc cagcgaagcc
    13441 agctggtcaa cctgccgctc ggcattacct ccacgccagg caatcaccag atcgggtttc
    13501 agcgcgacaa tgcgttccag attcatcccc tgccaggtgg aaacctgctc aatcttttgc
    13561 gcttgtggag gatagtcgga atagctgctg accccaaccg gcgtgatccc ggcggcaaag
    13621 gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt
    13681 ggcgcgagaa aagacagggc gaccagcgcc ctgaacagtg atttagccat gtgccagttt
    13741 ctgcaccagt gactcaacca tcaggctgga ctgtttagcg gcaacagcca gggactcatc
    13801 gaagctaaga tgagactgtt gatcggccac gtcggagatg gcgcgtacga caacaaacgg
    13861 gatgttgaaa ttgtggcaga catgggcgat tgccgtcgct tccatctcta cagcaatggc
    13921 ctgtgggaag ttgtggcgga ttttcgccag accaacagaa ccgttgatga aagcgtcgcc
    13981 gctaacaatc aggccacgta cagcgttaag attcagttcg gcaatgcagg cctcagcggc
    14041 agcgatcagt ttatcgtcag ctttaaagcc tgccggacag cctggtaact gaccgtattc
    14101 ataaccaaat gccgtgacat ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc
    14161 gcccactttc agcgttggtg ccaggcaacc ggcagaaccg gtgttaataa tcacatctgg
    14221 cttgcagtgt tccaacagca gagtggcacc cagcgccgca gcgactttac cgatgcccga
    14281 tttcagaagc gcaacctcgg ttccattcag ttggccggta tagatttcgc aaccgccgag
    14341 actgatagtt tgacggtttt cgattttgtc acgcagcagc gtaacttctt cttccattgc
    14401 accaatgatg ccgattttca tagatttact cgcgataagc ccgatttgaa ggcatagttt
    14461 accatgcgct tacggggaag cgtatttctc acgcgggaga ggacatggca cagattgatt
    14521 tccgaaaaaa aataaactgg catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac
    14581 atgagatcct gcggatcttc gagagcgatc gcgggcgtat catcaactct ccggcaattc
    14641 gtcgtctgca acaaaagacc caggtttttc cactggagcg caatgccgcc gtgcgcacgc
    14701 gtcttaccca ctcgatggaa gtccagcagg tgggacgcta catcgccaaa gaaattttaa
    14761 gccgtctgaa agagcttaaa ttgctggaag catacggcct ggatgaactg accggcccct
    14821 ttgaaagcat tgttgagatg tcatgcctga tgcacgatat cggcaatccg ccgtttggtc
    14881 attttggcga agcagcgata aatgactggt ttcgccaacg tttgcacccg gaagatgccg
    14941 aaagccagcc tctgactgac gatcgctgca gcgtggcggc actacgttta cgggacgggg
    15001 aagaaccgct taacgagctg cggcgcaaga ttcgtcagga cttatgtcat tttgagggga
    15061 atgcacaagg cattcgtctg gtgcatacat tgatgcggat aaatctcact tgggcacagg
    15121 ttggcggtat tttaaaatat acccgtccgg cgtggtggcg tggcgaaacg cctgagacac
    15181 atcactattt aatgaaaaag ccgggttatt atctttctga agaagcctat attgcccggt
    15241 tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc attaacgtgg attatggaag
    15301 ctgccgacga catctcctat tgtgtggcag accttgaaga tgcggtagag aaaagaatat
    15361 ttaccgttga gcagctttat catcatttgc acgaagcgtg gggccagcat gagaaaggtt
    15421 cgctcttttc gctggtggtt gaaaatgcct gggaaaaatc acgctcaaat agtttaagcc
    15481 gcagtacgga agatcagttt tttatgtatt tacgggtaaa caccctaaat aaactggtac
    15541 cctacgcggc acaacgattt attgataatc tgcctgcgat tttcgccgga acgtttaatc
    15601 atgcattatt ggaagatgcc agcgaatgca gcgatcttct taagctatat aaaaatgtcg
    15661 ctgtaaaaca tgtgtttagc catccggatg tcgagcagct tgaattgcag ggctatcggg
    15721 tcattagcgg attattagag atttatcgtc ctttattaag cctgtcgtta tcagacttta
    15781 ctgaactggt agaaaaagaa cgggtgaaac gtttccctat tgagtcgcgc ttattccaca
    15841 aactctcgac gcgccatcgg ctggcctatg tcgaggctgt cagtaaatta ccgtcagatt
    15901 ctcctgagtt tccgctatgg gaatattatt accgttgccg cctgctgcag gattatatca
    15961 gcggtatgac cgacctctat gcgtgggatg aataccgacg tctgatggcc gtagaacaat
    16021 aaccaggctt ttgtaaagac gaacaataaa tttttacctt ttgcagaaac tttagttcgg
    16081 aacttcgggc tataaaacga atctgaagaa cacagcaatt ttgcgttatc tgttaatcga
    16141 gactgaaata catgaaaaaa accacattag cactgagtgc actggctctg agtttaggtt
    16201 tggcgttatc tccgctctct gcaacggcgg ctgagacttc ttcagcaacg acagcccagc
    16261 agatgccaag ccttgcaccg atgctcgaaa aggtgatgcc ttcagtggtc agcattaacg
    16321 tagaaggtag cacaaccgtt aatacgccgc gtatgccgcg taatttccag cagttcttcg
    16381 gtgatgattc tccgttctgc caggaaggtt ctccgttcca gagctctccg ttctgccagg
    16441 ggggccaggg cggtaatggc ggcggccagc aacagaaatt catggcgctg ggttccggcg
    16501 tcatcattga tgccgataaa ggctatgtcg tcaccaacaa ccacgttgtt gataacgcga
    16561 cggtgattaa agtccagctg agcgatggtc gtaagttcga cgcgaagatg gttggcaaag
    16621 atccgcgctc tgatatcgcg ctgatccaaa tccagaaccc gaaaaacctg accgcaatta
    16681 agatggcgga ttctgatgca ctgcgcgtgg gtgattacac cgtagcgatt ggtaacccgt
    16741 ttggtctggg cgagacggta acttccggga ttgtctctgc gctggggcgt agcggcctga
    16801 atgctgaaaa ctacgaaaac ttcatccaga ccgatgcagc gatcaaccgt ggtaactccg
    16861 gtggtgcgct ggttaacctg aacggcgaac tgatcggtat caacaccgcg atcctcgcac
    16921 cggacggcgg caacatcggt atcggttttg ctatcccgag taacatggtg aaaaacctga
    16981 cctcgcagat ggtggaatac ggccaggtga aacgcggtga gctgggtatt atggggactg
    17041 agctgaattc cgaactggcg aaagcgatga aagttgacgc ccagcgcggt gctttcgtaa
    17101 gccaggttct gcctaattcc tccgctgcaa aagcgggcat taaagcgggt gatgtgatca
    17161 cctcactgaa cggtaagccg atcagcagct ttgccgcact gcgtgctcag gtgggtacta
    17221 tgccggtggg cagcaaactg accctgggct tactgcgcga cggtaagcag gttaacgtga
    17281 acctggaact gcagcagagc agccagaatc aggttgattc cagctccatc ttcaacggca
    17341 ttgaaggcgc tgagatgagc aacaaaggca aagatcaggg cgtggtagtg aacaacgtga
    17401 agacgggcac tccggctgcg cagatcggcc tgaagaaagg tgatgtgatt attggtgcga
    17461 accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt tctcgacagc aaaccgtctg
    17521 tgctggcact gaacattcag cgcggcgaca gcaccatcta cctgttaatg cagtaatctc
    17581 cctcaacccc ttcctgaata cgggaagggg ttctccttac aatctgtgaa cttcgccaca
    17641 actccataca tcttcatcat cctttgggca tttgcacaat gccgtacgtt acgtacttcc
    17701 ttatgctaag ccgtgcataa cggaggactt atggctggct ggcatcttga taccaaaatg
    17761 gcgcaggata tcgtggcacg taccatgcgc atcatcgata ccaatatcaa cgtaatggat
    17821 gcccgcgggc gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt gcacgaaggt
    17881 gcattgctgg tactttcaca gggacgagtc gtcgatatcg atgacgcggt agcacgtcat
    17941 ctgcacggtg tgcggcaggg gattaatcta ccgttacggc tggaaggtga aattgtcggc
    18001 gtaattggcc tgacaggtga accagagaat ctgcgtaaat atggcgaact ggtctgcatg
    18061 acggctgaaa tgatgctgga acagtcgcgg ttgatgcact tgttggcgca ggatagccgt
    18121 ttgcgggaag aactggtgat gaacctgatt caggcagagg agaatactcc cgcacttctg
    18181 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtggtggct attgttgagg
    18241 tcgacagcgg tcagcttggc gtggacagcg caatggcgga gttacaacaa ctgcaaaacg
    18301 cgctgactac gcccgagcgt aataatctgg tggcgattgt ctcgctaacc gaaatggtgg
    18361 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc agaagatcat cgtaagcgag
    18421 ttgaacaact gattacccgc atgaaagagt acggccagct gcgttttcgc gtttcactgg
    18481 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    18541 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    18601 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caacgaactg gcgcgaccgc
    18661 tggcgcggct gaaagcgatg gacaataacg gcttgctgcg acgaacgctg gcggcgtggt
    18721 ttcgccacaa tgtgcaaccg ctggcaacgt caaaggcgtt gtttattcat cgtaataccc
    18781 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggc aattttgatg
    18841 acaggttgct gctgtatgtg gcgttacaac tggatgaaga gcggtagatt atgcgttaag
    18901 gttgaggcgg cgctggctca ttcccctcag cccgaagggg agaggggaat gcccgagtta
    18961 accgtcaatt cgggattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    19021 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    19081 aacttcgctg cggtcacgct gacagatttg atcaagctca tcaatgatat accgtagatt
    19141 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    19201 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    19261 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    19321 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    19381 gtcagtaaat tgtgcttatt ttagcatttg gcttggcccg cccggctcga ctgtgatcgc
    19441 agaaagccgt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    19501 attgtggcga tattccatgg gatggattag cacatttatc ttcgttttgc gaattacatg
    19561 acagtccgaa acaagatgaa aaagtattta aaaaaggggg aggaagaatg agtaggacga
    19621 attattctga gccagaaagc atacgggcag catcatgctg cccgtatatg tactcttaat
    19681 cgatggtacg cagcagttcg ttaatgccga ctttgccgcg agttttcgcg tcaactttct
    19741 taacgataac cgcacagtag aggctgtatt tgccatcttt tgacggcaga ttacctgaaa
    19801 caaccacaga ccccgccgga acgcgaccgt agtggatttc gccggtttca cggtcgtaaa
    19861 tacgggtgct ctgaccaatg tatacgccca tagagatgac ggaaccttct tcgacaatca
    19921 ccccttcaac cacttcagag cgtgcgccaa tgaagcagtt atcttcaata atggtcgggt
    19981 tagcctgcag cggctccagc acgccgccga tgcccacgcc accggaaagg tggacgtttt
    20041 taccaatctg cgcacaagaa ccgacggtcg cccaggtatc aaccatggtg ccttcatcaa
    20101 catatgcgcc gatgttaacg taagacggca tcagcacggt gttacgggca ataaacgcac
    20161 cctgacgtac cgccgctggt ggcacaacgc ggaagccttc tttctggaaa cgtgcttcgt
    20221 cgtagtcggc gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca
    20281 cctgattatc attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca
    20341 cccactgacc gtcaattttt tccgctacac gcagtgcgcc ggaatccagc agggcgatca
    20401 cctgatttac cgcttcgcgg gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc
    20461 gttcaaaagc ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc
    20521 agtaaacaca tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt
    20581 cctgctgcaa ctcgttatta agcgcacgcc ggtcagcggt ggcaattatg aataaatctt
    20641 ctactcgctc gccaatggtt gtaattcggg caccatgaag cgaaattccc agatcggcaa
    20701 aaattttccc gactcgcgcc agcagtccag gttggtcgag ggcgatcagt tcgaggaacg
    20761 atttgcggtc ggtatgggtc ggcaaaaacg ttacttcggt ttcaacagta aaatggcgta
    20821 atttggcggg ttggcgacgg ggctgcggtg gctgccagct actttgcgtc agcacctgct
    20881 ccagaccaaa ccgaataacc tcatggcgat ctgcggacag cgggctgcca tcgggttcca
    20941 gcacgataaa ggtatccatc gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa
    21001 cacttaaatt gcggcggtct aattcggcac agacggcggc aaacagatag gggcggtccg
    21061 ggctccagat aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc aataccagcg
    21121 gtttgcttaa atcatgctgt aataaatggc gggcatgcca ggccagttga tttgggctat
    21181 ggcggacaaa atagttagca cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga
    21241 tgttatccat gcgcagtagt gccagtgcct ggagttggtg atggcgaacc cgttcgcgca
    21301 tatccggcgt gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc
    21361 gcaacagact ttgcttccag ctattccaca gcgtttcgtt ggtggcgcaa atgtcagcca
    21421 ctgtcaggca taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct
    21481 tgatgacttc agggtcctga atatcgcggc gttgtgctgt caccgacatc agcaagtgct
    21541 ggcgaaccag ccaggcaacc agctgtgttt cgcgtgagtt cagcccgtgg agttctgcaa
    21601 aatgcactac atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat
    21661 cgtgaaacag cgcggcgatg aaaatcagct cagttgacgg caggcgcggc cagacgtcca
    21721 cacacaacgg atggcgctgg cgcgtttctt cactggcaaa actctccagt ttcagcatca
    21781 cgcggatagt atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga
    21841 cgatatgcga ccattgcggc atatacgcgc cgagcacgct atggcgatgc attggcaata
    21901 gcccgcgccg caccgctccg gggtgacgca gaatgctcaa aaacagtttt cgtgcttgcg
    21961 gaatattaca cagcggttgt tgcagatggc ggcgggcatg gcgtaactgg cgcagcgtgg
    22021 tggagtaaat gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg
    22081 cttccggctg gcgcataaat agtgtttcat cacgcaggtc gattagcgta ccgcgtagct
    22141 gaaactcatc gtcgagtgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat
    22201 cgaacagttg cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat
    22261 ccttcatcat ccgctcgacc ggttcgttgc cttcaccact gtaattcaga cgctgagcga
    22321 cgctaagttg gcgatcgaat aacagacgat tatcgtaacg gctgacgacc agatgcaggg
    22381 caaagcgaat acgccacagt atatgcagac attcgtttaa ttccgcccgc tccgctgagg
    22441 ttaagaagcc aaacccgacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca
    22501 cccattgcag agtgtgaata tcgcgcaagc cgccagggct acttttgatg tctggttcaa
    22561 ggttgtagct ggtgccatgg taacgctgat ggcgctggtt ctgttcttca actttcgctg
    22621 cgtagaactt gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc agttcgagga
    22681 agagcgcaac atcgccaatt aataagcggg attcgattaa attggtggcg acggttaaat
    22741 ccgataaccc ttccagcatg cactcttcaa gcgtgcgcac actatgaccg acttccagct
    22801 ttacatccca gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct
    22861 ttttacggct taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt
    22921 agccaccgac ggcaaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata
    22981 atcgttgcag gagctggtct atgaactcgg tacgtgcctc aatcaactgt tctgcagaga
    23041 tcccattgtc aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc
    23101 cacagacggt taattcatca cggggccagg cgcatggatt ttgcggttga ccgggcaggg
    23161 tggggagagc ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag
    23221 attaaattat cgcattaaaa aagccggcat tcgccggctt catcttattc gtcgtgcgag
    23281 attatcgccg ggatggtgtc atccttgcgt agcgtcaaaa tttcgcagcc gttatcagtc
    23341 accacaatag tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag
    23401 ccatctttca tggtgcggat ctctttttta cccgcgttga ccattggctc aatggtgaac
    23461 gtcatccccg gtttcagcac gacgttggtt tcacgggagt catagtgcag cacctgcggt
    23521 tcttcatgga agccgcgacc aataccgtgt ccgcaatatt cacgaacgac ggagaagcct
    23581 tctgcttcga caaatttctg aatcgccgca ccgatttcgc gcagattaat gcctggtttt
    23641 accatgcgta gcgccaggta caggctttct tgcgtgatgc ggcacaggcg ttcgcccatg
    23701 atggtcggct taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt
    23761 acggtgacat caatgttaac gatatcgcca tctttcagca gcttagcatc gtccgggata
    23821 ccgtggcaca ccacttcatt aatagagatg caaacggatt tcggatagcc gtgatagccg
    23881 aggcaggcag aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccagc
    23941 tcgccggtgc tgacgcccgg tttaacatac ggttcgatca tctccagcac ttcggcagcc
    24001 agtcggccag cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt
    24061 aattctgtcc atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat
    24121 cacacccggg caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc
    24181 tataatagaa aattcgacgt ctgatgctgt acacagcgcc aacagttatt ggtgtccacg
    24241 acgtatttgt ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac
    24301 ggaagcgaca atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc
    24361 cctttggggt cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt
    24421 ttaatcatgg caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac
    24481 cagacccgtt actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt
    24541 cacatcatca accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac
    24601 aagattgttt ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa
    24661 gcggtgaaag acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc
    24721 ggtatgctga ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa
    24781 actcagtctc aggacggtac tttcgacaag ctgaccaaga aagaagcgct gatgcgcact
    24841 cgtgagctgg agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg
    24901 gacgctctgt ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac
    24961 ttgggtattc cggtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc
    25021 gttatcccgg gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct
    25081 gcaaccgtac gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta
    25141 gaagctgagt aataaggctt gataactccc ccaaaatagt tcgagttgca gaaaggcggc
    25201 aagctcgaga attcccggga gcttacatca gtaagtgacc gggatgagcg agcgaagaca
    25261 acgcatctgc ggcgcgaaat atgaaggggg agagccctta tagaccaggt agtacacgtt
    25321 tggttagggg gcctgcatat ggcccccttt ttcactttta tatctgtgcg gtttaatgcc
    25381 gggcagatca catctccgag gattttagaa tggctgaaat taccgcatcc ctggtaaaag
    25441 agctgcgtga gcgtactggc gcaggcatga tggattgcaa aaaagcactg actgaagcta
    25501 acggcgacat cgagctggca atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga
    25561 aaaaagcagg caacgttgct gctgacggcg tgatcaaaac caaaatcgac ggcaactacg
    25621 gcatcattct ggaagttaac tgccagactg acttcgttgc aaaagacgct ggtttccagg
    25681 cgttcgcaga caaagttctg gacgcagctg ttgctggcaa aatcactgac gttgaagttc
    25741 tgaaagcaca gttcgaagaa gaacgtgttg cgctggtagc gaaaattggt gaaaacatca
    25801 acattcgccg cgttgctgcg ctggaaggcg acgttctggg ttcttatcag cacggtgcgc
    25861 gtatcggcgt tctggttgct gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta
    25921 tgcacgttgc tgcaagcaag ccagaattca tcaaaccaga agacgtatcc gctgaagtgg
    25981 tagaaaaaga ataccaggta cagctggata tcgcgatgca gtctggtaag ccgaaagaaa
    26041 tcgcagagaa aatggttgaa ggccgcatga agaaattcac cggcgaagtt tctctgaccg
    26101 gtcagccgtt cgttatggaa ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg
    26161 ctgaagtgac tggcttcatc cgtttcgaag tgggtgaagg catcgagaaa gttgagactg
    26221 actttgcagc agaagttgct gcgatgtcca agcagtctta attatcaaaa aggagccgcc
    26281 tgagggcggc ttctttttgt gcccatcttg taaattcagc taacccttgt ggggctgcgc
    26341 tgaaaagcga cgtacaatgt cgctagtatt aattcatttc aatcgttgac agtctcagga
    26401 aagaaacatg gctaccaatg caaaacccgt ctataaacgc attctgctta agttgagtgg
    26461 cgaagctctg cagggcactg aaggcttcgg tattgatgca agcatactgg atcgtatggc
    26521 tcaggaaatc aaagaactgg ttgaactggg tattcaggtt ggtgtggtga ttggtggggg
    26581 taacctgttc cgtggcgctg gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca
    26641 catggggatg ctggcgaccg taatgaacgg cttggcaatg cgtgatgcac tgcaccgcgc
    26701 ctatgtgaac gctcgtctga tgtccgctat tccattgaat ggcgtgtgcg acagctacag
    26761 ctgggcagaa gctatcagcc tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac
    26821 aggtaacccg ttctttacca ccgactcagc agcttgcctg cgtggtatcg aaattgaagc
    26881 cgatgtggtg ctgaaagcaa ccaaagttga cggcgtgttt accgctgatc cggcgaaaga
    26941 tccaaccgca accatgtacg agcaactgac ttacagcgaa gtgctggaaa aagagctgaa
    27001 agtcatggac ctggcggcct tcacgctggc tcgtgaccat aaattaccga ttcgtgtttt
    27061 caatatgaac aaaccgggtg cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt
    27121 aatcacggaa taattcccgt gatggataaa taagggtaag attccgcgta agtatcgcgg
    27181 gggcgtaagt ctggttataa ggcgttattg ttgcaggcag tttggtcacg gccagcgcgc
    27241 agcaaccgga gcgtacaaaa gtacgtgagg atggcgagca ctgctcgggg ccaaaatggc
    27301 aaataaaata gcctaataat ccagacgatt acccgtaata tgtttaatca gggctatact
    27361 tagcacactt ccactgtgtg tgactgtctg gtctgactga gacaagtttt caaggattcg
    27421 taacgtgatt agcgatatca gaaaagatgc tgaagtacgc atggacaaat gcgtagaagc
    27481 gttcaaaacc caaatcagca aaatacgcac gggtcgtgct tctcccagcc tgctggatgg
    27541 cattgtcgtg gaatattacg gcacgccgac gccgctgcgt cagctggcaa gcgtaacggt
    27601 agaagattcc cgtactctga aaatcaacgt gtttgatcgt tcaatgtctc cggccgttga
    27661 aaaagcgatt atggcctccg atctcggcct gaacccgaac tctgcgggta gcgacatccg
    27721 tgttccgctg ccgccgctga cggaagaacg tcgtaaagat ctgaccaaaa tcgttcgtgg
    27781 tgaagcagaa caagcgcgtg ttgcagtacg taatgtgcgt cgtgacgcga acgacaaagt
    27841 gaaagcactg ttgaaagata aagagatcag cgaagacgac gatcgccgtt ctcaggacga
    27901 tgtacagaaa ctgactgatg ctgcaatcaa gaaaattgaa gcggcgctgg cagacaaaga
    27961 agcagaactg atgcagttct gatttcttga acgacaaaaa cgccgctcag tagatccttg
    28021 cggatcggct ggcggcgttt tgctttttat tctgtctcaa ctctggatgt ttcatgaagc
    28081 aactcaccat tctgggctcg accggctcga ttggttgcag cacgctggac gtggtgcgcc
    28141 ataatcccga acacttccgc gtagttgcgc tggtggcagg caaaaatgtc actcgcatgg
    28201 tagaacagtg cttggaattc tctccccgct atgccgtaat ggacgatgaa gcgagtgcga
    28261 aacttcttaa aacgatgcta cagcaacagg ggagccgcac cgaagtctta agtgggcaac
    28321 aagccgcttg cgatatggca gcgcttgagg aggttgatca ggtgatggca gccattgttg
    28381 gtgctgctgg actgttacct acgcttgctg cgatccgcgc gggtaaaacc attttgctgg
    28441 ccaataaaga atcactggtt acctgcggac gtctgtttat ggacgccgta aagcagagca
    28501 aagcgcaatt gttaccggtc gatagcgaac ataacgctat ttttcagagt ttaccgcaac
    28561 ctatccagca taatctggga tacgctgacc ttgagcaaaa tggcgtggtg tccattttac
    28621 ttaccgggtc tggtggccct ttccgtgaga cgccattgcg cgatttggca acaatgacgc
    28681 ccgatcaagc ttgccgtcat ccgaactggt cgatgggacg taaaatttct gtcgattcgg
    28741 ctaccatgat gaacaaaggt ctggaataca ttgaagcgcg ttggctgttt aacgccagcg
    28801 ccagccagat ggaagtgctg attcacccgc agtcagtgat tcactcaatg gtgcgctatc
    28861 aggacggcag tgttctggcg cagctggggg aaccggatat gcgtacgcca attgcccaca
    28921 ccatggcatg gccgaatcgc gtgaactctg gcgtgaagcc gctcgatttt tgcaaactaa
    28981 gtgcgttgac atttgccgca ccggattatg agcgttatcc atgcctgaaa ctggcgatgg
    29041 aggcgttcga acaaggccag gcagcgacga cagcattgaa tgccgcaaac gaaatcaccg
    29101 ttgctgcttt tcttgcgcaa caaatccgct ttacggatat cgctgcgttg aatttatccg
    29161 tactggaaaa aatggatatg cgcgaaccac aatgtgtgga cgatgtgtta tctgttgatg
    29221 cgaacgcgcg tgaagtcgcc agaaaagagg tgatgcgtct cgcaagctga ggataatccg
    29281 gctacagaga gtcgcgctat ttgttagcgt agggcttcag tgatatagtc tgcgccatct
    29341 gatcgtaagt agttggcttt ataaggtcag gtatgccgtg gttttacacg gctttttttt
    29401 gtataggctt cagtattcct gagtaccgta aaccctgtca gggaataaaa aacgcgtgat
    29461 gttgtctgct actcaaccac ttagcgaaaa attgccagcg catggctgcc gtcatgttgc
    29521 gatcattatg gacggcaatg gtcgctgggc aaaaaagcaa gggaagattc gtgcctttgg
    29581 gcataaagcc ggggcaaaat ccgtccgccg ggctgtctct tttgcggcaa acaacggtat
    29641 tgaggcgtta acgctgtatg cctttagtag tgaaaactgg aaccgaccag cgcaggaagt
    29701 cagtgcgtta atggaactgt ttgtgtgggc gctcgatagc gaagtaaaaa gtctgcaccg
    29761 acataacgtg cgtctgcgta ttattggcga taccagtcgt tttaactcgc gtttgcaaga
    29821 acgtattcgt aaatctgaag cgctaacagc cggaaatacc ggtctgacgc tgaatattgc
    29881 ggcgaactac ggtggacgtt gggatatagt ccagggagtc aggcaactgg ctgaaaaggt
    29941 gcagcaagga aacctgcaac cagatcagat agatgaagag atgttaaacc agcatgtctg
    30001 tatgcatgaa ctggcccctg tagatttagt aattaggact gggggagagc atcgcattag
    30061 taactttttg ctttggcaaa ttgcctatgc cgaactttac tttacagatg ttctctggcc
    30121 cgatttcgat gaacaagact ttgaaggggc gttaaatgcc tttgctaatc gagagcgtcg
    30181 tttcggcggc accgagcccg gtgatgaaac agcctgatgg gggtcgcttt tgctgaagta
    30241 tcgcctgata tctgcttttg tgttaatacc cgtcgtcatc gcggcgttgt ttctgttgcc
    30301 gccggtgggg ttcgccattg taacgctggt ggtctgcatg ctggcagcgt gggaatgggg
    30361 acagcttagc ggttttacca ctcgttcgca gcgagtatgg ttggcggtgt tatgcgggtt
    30421 attgttggcg ctgatgcttt ttctgttgcc ggaatatcac cgaaatattc atcaaccgct
    30481 ggttgaaatc tcactttggg cttcgttggg ttggtggatt gtcgcgctat tgctggtgct
    30541 gttttaccca ggttccgcag caatctggcg taactctaaa acattgcgcc ttatttttgg
    30601 cgtgctaacc attgttccct tcttctgggg catgctggcg ttacgggcct ggcactatga
    30661 cgagaatcat tacagtggcg caatatggct gctctatgtc atgatcctgg tatggggcgc
    30721 tgactccggc gcatatatgt ttggcaaatt gtttggtaaa cataagctgg caccgaaggt
    30781 ttctccgggt aaaacctggc aaggctttat aggtggactc gctactgcag cggtaatctc
    30841 atggggttat ggcatgtggg cgaatctcga cgtcgctccc gtcaccttac tcatttgctc
    30901 tattgtcgca gcgttagcct cagtgctcgg cgatctgacc gagagtatgt ttaagcgtga
    30961 agcaggaatt aaggacagcg gtcatttaat tccaggacac ggtggtattt tagatcgtat
    31021 tgatagcctg acggctgcgg taccggtctt tgcttgcttg ttgttactgg tattcaggac
    31081 gctttaacgg aaggtaatat gctgagtttt ctctgggatt tggcttcgtt catcgttgca
    31141 ctgggtgtac ttatcaccgt gcatgaattt ggtcatttct gggttgcccg gcgttgtggt
    31201 gttcgcgttg agcgtttctc aatagggttt ggtaaggcgc tctggcggcg aactgataag
    31261 ctcggcaccg aatatgttat cgccctgatc ccgttgggcg gttatgtcaa aatgctggat
    31321 gagcgcgcag aaccggtcgt tccggaactc cgccaccatg ccttcaataa taaatctgtc
    31381 ggccaacgag cggcgattat tgccgcaggt ccggttgcaa acttcatttt tgctatcttt
    31441 gcctactggc tggtttttat tattggtgtg cctggcgtac gtccggtggt tggcgaaata
    31501 gcagccaatt cgatagctgc ggaagcacaa attgcaccag gtacggaact aaaagccgta
    31561 gatggtatcg aaacgcctga ttgggatgcc gtgcgtttgc agttggtcga taaaattggc
    31621 gatgaaagca ccaccattac agtagcgcca tttggcagcg accaacggcg ggatgtaaag
    31681 ctcgatttac gtcactgggc gtttgagcct gataaagaag atccggtatc ttcgctgggg
    31741 attcgtcctc gtgggccgca aattgaacct gtactggaaa atgtgcagcc aaactcggcg
    31801 gcaagcaagg caggtttgca agcaggcgac aggatcgtta aagtcgatgg tcagccctta
    31861 acgcagtggg tgacctttgt gatgtttgtc cgggataacc cgggtaaatc cttagcgtta
    31921 gaaatcgaaa ggcaggggag tcccttgtct ttgacattaa tcccggagag taaaccgggt
    31981 aatggtaaag cgattggttt tgtcggtatt gagccgaaag tcattccttt gccagatgag
    32041 tataaagttg tacgccagta tgggccgttc aacgccatcg tcgaagccac ggacaaaacg
    32101 tggcagctga tgaagctgac ggtcagtatg ctgggaaaat tgatcaccgg tgatgtgaaa
    32161 ctgaacaacc tcagtgggcc gatctctatc gccaaggggg ctgggatgac agcggaactc
    32221 ggggtagttt attacctgcc gtttcttgcg cttattagcg tgaacttagg gataattaac
    32281 ctgtttccgt tgcccgtact tgacgggggg catctgctgt tccttgcgat cgaaaagatc
    32341 aagggcggac cggtatccga gcgggttcaa gacttttgtt atcgcattgg ctcgattctg
    32401 ctggtgctgt taatggggct tgcacttttc aatgatttct ctcggttatg agagttagtt
    32461 aggaagaacg cataataacg atggcgatga aaaagttgct catagcgtcg ctgctgttta
    32521 gcagcgccac cgtatacggt gctgaagggt tcgtagtgaa agatattcat ttcgaaggcc
    32581 ttcagcgtgt cgccgttggt gcggccctcc tcagtatgcc ggtgcgtaca ggcgacacgg
    32641 ttaatgatga agatatcagt aataccattc gcgctctgtt tgctaccggc aactttgagg
    32701 atgttcgcgt ccttcgtgat ggtgataccc ttctggttca ggtaaaagaa cgtccgacca
    32761 ttgccagcat tactttctcc ggtaacaaat cggtgaaaga tgacatgctg aagcaaaacc
    32821 tcgaggcttc tggtgtgcgt gtgggcgaat ccctcgatcg caccaccatt gccgatatcg
    32881 agaaaggtct ggaagacttc tactacagcg tcggtaaata tagcgccagc gttaaagctg
    32941 tcgtgacccc gctgccgcgc aaccgtgttg acctaaaact ggtgttccag gaaggtgtgt
    33001 cagctgaaat ccagcaaatt aacattgttg gtaaccatgc tttcaccacc gacgaactga
    33061 tctctcattt ccaactgcgt gacgaagtgc cgtggtggaa tgtggtaggc gatcgtaaat
    33121 accagaaaca gaaactggcg ggcgaccttg aaaccctgcg cagctactat ctggaccgcg
    33181 gttatgcccg tttcaacatc gactctaccc aggtcagtct gacgccagat aaaaaaggta
    33241 tttacgtcac ggtgaacatc accgaaggcg atcagtacaa gctttctggc gttgaagtga
    33301 gcggcaacct tgccgggcac tccgctgaaa ttgagcagct gactaagatc gagccgggcg
    33361 agctgtataa cggcaccaaa gtgaccaaaa tggaagatga cattaaaaag cttctcggtc
    33421 gctatggtta tgcctatccg cgcgtacagt cgatgcctga aattaacgat gccgacaaaa
    33481 ccgttaaatt acgcgtgaac gttgatgcgg gtaaccgttt ctacgtgcgt aagatccgtt
    33541 ttgaaggtaa cgatacctcg aaagatgccg tcctgcgtcg cgaaatgcgt cagatggaag
    33601 gtgcatggtt ggggagcgat ctggtcgatc agggtaagga gcgtctgaat cgtctgggct
    33661 tctttgaaac tgtcgatacc gatacccaac gtgttccggg tagcccggac caggttgatg
    33721 tagtctacaa ggtgaaagaa cgtaacactg gtagcttcaa cttcgggatt ggttacggta
    33781 ctgaaagtgg cgtgagcttc caggctggcg tgcaacagga taactggtta ggtacaggtt
    33841 atgctgttgg tatcaacggg accaaaaacg attaccagac ctatgctgaa ctgtcggtaa
    33901 ccaacccgta cttcaccgta gatggcgtaa gcctcggtgg tcgtctcttc tataatgact
    33961 tccaggcaga tgacgccgac ctgtccgact ataccaacaa gagttatggt acagacgtga
    34021 cgttgggctt cccgattaac gaatataact cgctgcgtgc aggtctgggt tatgtacata
    34081 actccctgtc caacatgcag cctcaggttg cgatgtggcg ttatctgtac tctatgggtg
    34141 aacatccgag cacctctgat caggataaca gcttcaaaac ggacgacttc acgttcaact
    34201 atggttggac ctataacaag cttgaccgtg gttacttccc gacagatggt tcacgtgtca
    34261 acctgaccgg taaagtgacc attcctggat cggataacga atactacaaa gtgacgttag
    34321 acacggcgac ttatgtgccg atcgatgacg atcacaaatg ggttgttctg ggtcgtaccc
    34381 gctggggtta tggtgatggt ttaggcggca aagagatgcc gttctacgag aacttctatg
    34441 ccggtggttc cagcaccgtg cgtggcttcc agtccaatac cattggtccg aaagcagttt
    34501 acttcccgca tcaggccagt aattatgatc cggactatga ttacgaatgt gcgactcagg
    34561 acggcgcgaa agacctgtgt aaatcggatg atgctgtagg cggtaacgcc atggcggttg
    34621 ccagcctcga gttcatcacc ccgacgccgt ttattagcga taagtatgct aactcggttc
    34681 gtacttcatt cttctgggat atgggtaccg tttgggatac aaactgggat tccagccaat
    34741 attctggata tccggactat agtgatccaa gcaatatccg tatgtctgcg ggtatcgcat
    34801 tacaatggat gtccccattg gggccgttgg tgttctccta cgcccagccg ttcaaaaagt
    34861 acgatggaga caaggcagaa cagttccagt ttaacatcgg taaaacctgg taagtgttct
    34921 ccacaaagga atgtagtggt agtgtagcga tgactttagg cgatcaatat aagatcgccg
    34981 ggccacgcaa agaactgcac cctccggtgc aaatgggatg gtaaggagtt tattgtgaaa
    35041 aagtggttat tagctgcagg tcttggttta gcactggcaa cttctgctca ggcggctgac
    35101 aaaattgcaa tcgtcaatat gggcagcctg ttccagcagg tagcgcagaa aaccggtgtt
    35161 tctaacacgc tggaaaatga gttcaaaggc cgtgccagcg aactgcagcg tatggaaacc
    35221 gatctgcagg ctaaaatgaa aaaactgcag tccatgaaag cgggcagcga tcgcaccaaa
    35281 ctggaaaaag acgtgatggc tcagcgccag acttttgctc agaaagcgca ggcttttgag
    35341 caggatcgcg cacgtcgttc caacgaagaa cgcggcaaac tggttactcg tatccagact
    35401 gctgtgaaat ccgttgccaa cagccaggat atcgatctgg ttgttgatgc aaacgccgtt
    35461 gcttacaaca gcagcgatgt aaaagacatc actgccgacg tactgaaaca ggttaaataa
    35521 gtaatgcctt caattcgact ggctgattta gcgcagcagt tggatgcaga actacacggt
    35581 gatggcgata tcgtcatcac cggcgttgcg tccatgcaat ctgcacaaac aggtcacatt
    35641 acgttcatgg ttaacccaaa ataccgtgag catttaggct tgtgccaggc gtccgcggtt
    35701 gtcatgaccc aggacgatct tcctttcgcg aaaagtgccg cgctggtagt gaagaatccc
    35761 tacctgactt acgcgcgcat ggcgcaaatt ttagatacca cgccgcagcc cgcgcagaac
    35821 attgcaccca gtgcggtgat cgacgcgacg gcgaagctgg gtaacaacgt atcgattggc
    35881 gctaacgcgg tgattgagtc cggcgttgaa ctgggcgata acgtgattat cggtgccggt
    35941 tgcttcgtag gtaaaaacag caaaatcggt gcaggttcgc gtctctgggc gaacgtaacc
    36001 atttaccatg agatccagat cggtcagaat tgcctgatcc agtccggaac agtggtaggc
    36061 gcagacggct ttggttatgc caacgatcgt ggtaactggg tgaagatccc acagattggt
    36121 cgcgtaatta ttggcgatcg cgtggagatc ggtgcctgca caaccatcga tcgcggcgcg
    36181 ctggatgaca ctgttattgg caatggcgtg atcattgata accagtgcca gattgcacat
    36241 aacgtcgtga ttggcgacaa tacggcggtt gccggtggcg tcattatggc gggcagcctg
    36301 aaaattggtc gttactgcat gatcggcgga gccagcgtaa tcaacgggca tatggaaata
    36361 tgcgacaaag tgacggttac gggcatgggt atggtgatgc gtcccatcac tgaaccaggc
    36421 gtctattcct caggcattcc gctgcaaccc aacaaagtct ggcgcaaaac cgctgcactg
    36481 gtgatgaaca ttgatgacat gagcaagcgt ctgaaatcgc ttgagcgcaa ggttaatcaa
    36541 caagactaac gttccatctt ttgttcgcca aactttacgg cctgtctcat tcttacgatt
    36601 gcggcaggcc gtgttattat tgtcgtttct tatattttga caggaagagt atcttgacta
    36661 ctaacactca tactctgcag attgaagaga ttttagaact tctgccgcac cgtttcccgt
    36721 tcttactggt ggatcgcgtg ctggattttg aagaaggtcg ttttctgcgc gcagtaaaaa
    36781 atgtctctgt caatgagcca ttcttccagg gccatttccc tggaaaaccg attttcccgg
    36841 gtgtgctgat tctggaagca atggcacagg caacaggtat tctggcgttt aaaagcgtag
    36901 gaaaactgga accgggtgag ctgtactact tcgctggtat tgacgaagcg cgcttcaagc
    36961 gcccggtcgt gcctggcgat caaatgatca tggaagtcac tttcgaaaaa acgcgccgcg
    37021 gcctgacccg ttttaaaggg gttgctctgg tcgatggtaa agtagtttgc gaagcaacga
    37081 tgatgtgtgc tcgtagccgg gaggcctgat acgtgattga taaatccgcc tttgtgcatc
    37141 caaccgccat tgtggaagag ggcgcgtcaa ttggcgcgaa cgcacacatt ggtccttttt
    37201 gtatcgttgg accccatgtc gaaattggtg agggtaccgt actgaaatct cacgttgtcg
    37261 tgaatggtca tactaaaatt ggccgcgata atgagattta tcagtttgcc tccatcggcg
    37321 aagttaacca ggatttgaaa tatgctggcg aaccgacccg tgtggaaatc ggcgatcgta
    37381 accgcattcg cgaaagcgtc accattcatc gtggcacagt ccagggcggt ggattgacga
    37441 aggtgggcag cgacaactta ctgatgatca acgcgcacat tgcgcacgat tgtacggtag
    37501 gtaaccgctg tattctcgcc aacaacgcaa cgctggcggg tcacgtatcg gttgacgact
    37561 tcgcgatcat cggcggcatg accgcagtcc atcagttctg catcattggt gcgcatgtga
    37621 tggttggcgg ctgctccggt gtggcgcagg acgtccctcc ttatgtcatt gcgcagggta
    37681 accacgcaac gccgttcggt gtcaatatcg aagggctgaa gcgccgcgga ttcagccgtg
    37741 aggcgattac cgctatccgc aatgcgtata agctaattta tcgtagcggt aaaacgctcg
    37801 atgaagtgaa accggaaatt gctgaactgg cggaaacata tccggaagtg aaagccttta
    37861 ccgatttctt tgcacgctca acgcgcggtc tgattcgtta atgactgacc agcgtccatt
    37921 aacgattgcc ctggtcgccg gagaaacctc cggcgatatc ctgggggccg gtttaatccg
    37981 cgctctgaaa gaacgtgtgc ccaacgcccg ctttgttggt gttgccgggc cacgaatgca
    38041 ggctgaaggc tgcgaagcct ggtacgaaat ggaagaactg gcggtgatgg gcattgttga
    38101 agtgctcggt cgtctgcgtc gcttactgca tattcgtgcc gatctgacaa agcgttttgg
    38161 cgaactgaag ccagatgttt ttgttggtat tgatgcgccc gacttcaata ttactcttga
    38221 aggtaacctc aaaaagcagg gtatcaaaac cattcattac gtcagtccat ccgtctgggc
    38281 gtggcgacag aaacgcgttt tcaaaatagg cagagccacc gatctggtgc tcgcatttct
    38341 gcctttcgaa aaagcgtttt atgacaaata caatgtaccg tgccgcttta tcggtcatac
    38401 catggctgat gccatgccgt tagatccaga taaaaatggt gcccgtgatg tgctggggat
    38461 cccttacgat gcccactgtc tggcattgtt gccgggcagc cgtggtgcag aagtcgaaat
    38521 gcttagtgcc gatttcttga aaactgccca gcttttgcgc cagacatatc cggatctcga
    38581 aatcgtggtg ccgctggtga atgccaaacg ccgcgagcag tttgaacgca tcaaagctga
    38641 agtcgcgcca gacctgtcgg ttcatttgct ggatggaatg ggccgtgagg cgatggtcgc
    38701 cagcgatgcg gcactactgg cgtcggggac ggcagccctg gagtgtatgc tggcgaaatg
    38761 cccgatggtg gtgggatatc gcatgaagcc ttttaccttc tggttggcga agcggctggt
    38821 gaaaactgat tatgtctcgc tgccaaatct gctggcgggc agagagttag tcaaagagtt
    38881 attgcaggaa gagtgtgagc cgcaaaaact ggctgcggcg ctgttaccgc tgttggcgaa
    38941 cgggaaaacc agccacgcga tgcacgatac cttccgtgaa ctgcatcagc agatccgctg
    39001 caatgccgat gagcaggcgg cacaagccgt tctggagtta gcacaatgat cgaatttgtt
    39061 tatccgcaca cgcagctggt tgcgggtgtg gatgaagtcg gacgcgggcc gttagttggc
    39121 gcggtcgtca ccgctgcggt gatccttgac ccggcgcgcc cgattgccgg gctgaatgat
    39181 tccaaaaagc tgagcgaaaa acgccgtctg gcgctctatg aagagatcaa agagaaagcg
    39241 ttgagctgga gtctgggccg cgcggaaccc cacgaaatcg acgagctgaa cattcttcat
    39301 gcgaccatgc tggcgatgca gcgtgccgtc gctgggctgc atattgcgcc ggaatatgtg
    39361 ttgattgatg gtaaccgctg cccgaaatta ccgatgcctg cgatggctgt ggtgaaaggg
    39421 gatagccgcg taccagaaat cagtgccgcg tctatcctgg cgaaagtgac gcgtgacgcc
    39481 gaaatggcgg cgctggatat tgttttcccg caatatggtt ttgcccaaca taaagggtat
    39541 ccaaccgctt ttcatctgga aaaactggct gaatatggcg cgactgaaca tcatcgtcgc
    39601 agctttgggc ctgtcaaacg cgcactggga cttgcgtcct gattcttgtg tcgagattaa
    39661 gtaaaccgga atctgaagat gtctgaacca cgtttcgtac acctgcgggt gcacagcgac
    39721 tactcgatga tcgatggctt ggccaaaacc gcaccgctgg taaaaaaggc ggcggcgttg
    39781 ggtatgccag cactggcgat caccgatttc accaaccttt gcggtctggt gaagttctac
    39841 ggagcgggac atggcgcagg gattaagcct atcgtcgggg cagattttaa tgtccagtgc
    39901 gacctgctgg gtgatgagtt aacccacctg acggtactgg cggcgaacaa taccggctat
    39961 cagaatctgg cgttgctgat ctcaaaagcg tatcagcgcg ggtacggtgc cgccgggccg
    40021 atcatcgatc gcgactggct tatcgaatta aacgaagggt tgatccttct ttccggcggg
    40081 cgcatgggcg acgtcggacg cagtcttttg cgtggtaaca gcgcgctggt agatgagtgt
    40141 gtcgcgtttt atgaagaaca cttcccggat cgctattttc tcgagctgat ccgcaccggc
    40201 aggccggatg aagaaagcta tctgcacgcg gcggtggaac tggcggaagc gcgcggtttg
    40261 cccgtcgtgg cgactaacga tgtgcgcttt atcgacagca gcgactttga cgcacacgaa
    40321 atccgcgtcg cgatccacga cggctttacc ctcgacgatc ctaaacgccc gcgtaactat
    40381 tcgccgcagc aatatatgcg tagcgaagag gagatgtgcg agctgtttgc cgacatccca
    40441 gaagcccttg ccaacaccgt tgagatcgcc aaacgctgta acgtaactgt gcgtcttggt
    40501 gaatacttcc tgccgcagtt cccgaccggg gacatgagca ccgaagatta tctggtcaag
    40561 cgtgcaaaag aaggcctgga agagcgtctg acatttttat ttcctgacga ggaagaacgt
    40621 cttaaacgcc gcccggaata tgacgaacgt ctggagactg aacttcaggt tatcaaccag
    40681 atgggcttcc cgggctactt cctcatcgtt atggaattta tccagtggtc gaaagataac
    40741 ggcgtaccgg tagggccagg ccgtggctcc ggtgcgggtt cactggtagc ctacgcgctg
    40801 aaaatcaccg acctcgatcc gctggaattt gacctgctgt tcgaacgttt ccttaacccg
    40861 gaacgtgtct ccatgcctga cttcgacgtt gacttctgta tggagaaacg cgatcaggtt
    40921 atcgaacacg tggcggacat gtacggtcgt gatgcggtat cgcagattat caccttcggt
    40981 acgatggcgg cgaaagcggt gatccgcgac gtaggccgcg tgctggggca tccgtacggc
    41041 tttgtcgacc gtatctcgaa actgatcccg cccgatccgg ggatgacgct ggcgaaagcg
    41101 tttgaagccg agccgcagct gccggaaatc tacgaagcgg atgaagaagt taaggcgctg
    41161 atcgacatgg cgcgcaaact ggaaggggtc acccgtaacg ccggtaagca cgccggtggg
    41221 gtggttatcg cgccgaccaa aattaccgat tttgcgccgc tttattgcga tgaagagggc
    41281 aaacatccgg tcacccagtt tgataaaagc gacgttgaat acgccggact ggtgaagttc
    41341 gacttccttg gtttgcgtac gctcaccatc attaactggg cgctggagat gatcaacaag
    41401 cggcgggcga agaatggcga gccaccgctg gatatcgccg cgatcccgct ggatgacaag
    41461 aaaagcttcg acatgctgca acgctcggaa accacggcgg tattccagct tgaatcgcgc
    41521 ggcatgaagg acctgatcaa gcgtctgcaa cctgactgct tcgaagatat gatcgcactg
    41581 gtggcactgt tccgccctgg tccgttgcag tcagggatgg tggataactt tatcgaccgt
    41641 aaacatggtc gtgaagagat ctcctatccg gacgtgcagt ggcagcatga aagcctgaaa
    41701 tcggtactgg agccaaccta cggcattatc ttgtatcagg aacaggtcat gcagattgcc
    41761 caggtgcttt ctggttatac cctcggtggc gcggatatgc tgcgccgtgc gatgggtaag
    41821 aaaaagccgg aagagatggc taagcagcgt tctgtatttg ctgaaggtgc agaaaagaac
    41881 ggaatcaacg ccgaactggc gatgaaaatc ttcgacctgg tggagaaatt cgcgggttac
    41941 ggatttaaca aatcgcactc tgcggcctat gctttggtgt catatcaaac gttatggctg
    42001 aaagcgc
//
LOCUS       DADLSE010000019        41546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLSE010000019 DADLSE010000000
VERSION     DADLSE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.019"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            13..89
                     /locus_tag="JN692_001025"
     tRNA            13..89
                     /locus_tag="JN692_001025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Met,seq:cat)
     gene            138..212
                     /locus_tag="JN692_001026"
     tRNA            138..212
                     /locus_tag="JN692_001026"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:170..172,aa:Gln,seq:ctg)
     gene            250..324
                     /locus_tag="JN692_001027"
     tRNA            250..324
                     /locus_tag="JN692_001027"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:282..284,aa:Gln,seq:ctg)
     gene            complement(478..1653)
                     /gene="ubiF"
                     /locus_tag="JN692_001028"
     CDS             complement(478..1653)
                     /gene="ubiF"
                     /locus_tag="JN692_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7263398.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVIPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1799..3223
                     /gene="miaB"
                     /locus_tag="JN692_001029"
     CDS             1799..3223
                     /gene="miaB"
                     /locus_tag="JN692_001029"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7263399.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3337..4416
                     /locus_tag="JN692_001030"
     CDS             3337..4416
                     /locus_tag="JN692_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7263400.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYQAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4413..4880
                     /gene="ybeY"
                     /locus_tag="JN692_001031"
     CDS             4413..4880
                     /gene="ybeY"
                     /locus_tag="JN692_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7263401.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4970..5848
                     /gene="corC"
                     /locus_tag="JN692_001032"
     CDS             4970..5848
                     /gene="corC"
                     /locus_tag="JN692_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7263402.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5873..7411
                     /gene="lnt"
                     /locus_tag="JN692_001033"
     CDS             5873..7411
                     /gene="lnt"
                     /locus_tag="JN692_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7263403.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7808..8716
                     /gene="gltI"
                     /locus_tag="JN692_001034"
     CDS             7808..8716
                     /gene="gltI"
                     /locus_tag="JN692_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HAY7263404.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8886..9626
                     /gene="gltJ"
                     /locus_tag="JN692_001035"
     CDS             8886..9626
                     /gene="gltJ"
                     /locus_tag="JN692_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HAY7263405.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            9626..10300
                     /gene="gltK"
                     /locus_tag="JN692_001036"
     CDS             9626..10300
                     /gene="gltK"
                     /locus_tag="JN692_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7263406.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10300..11025
                     /gene="gltL"
                     /locus_tag="JN692_001037"
     CDS             10300..11025
                     /gene="gltL"
                     /locus_tag="JN692_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7263407.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11143..12078
                     /gene="rihA"
                     /locus_tag="JN692_001038"
     CDS             11143..12078
                     /gene="rihA"
                     /locus_tag="JN692_001038"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7263408.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            12162..13832
                     /locus_tag="JN692_001039"
     CDS             12162..13832
                     /locus_tag="JN692_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7263409.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(13892..15343)
                     /gene="djlC"
                     /locus_tag="JN692_001040"
     CDS             complement(13892..15343)
                     /gene="djlC"
                     /locus_tag="JN692_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7263410.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(15340..16047)
                     /locus_tag="JN692_001041"
                     /pseudo
     CDS             complement(15340..16047)
                     /locus_tag="JN692_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            16149..16703
                     /locus_tag="JN692_001042"
     CDS             16149..16703
                     /locus_tag="JN692_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7263411.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16713..18140)
                     /locus_tag="JN692_001043"
     CDS             complement(16713..18140)
                     /locus_tag="JN692_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7263412.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18137..18844)
                     /locus_tag="JN692_001044"
     CDS             complement(18137..18844)
                     /locus_tag="JN692_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7263413.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHLSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            19008..19988
                     /locus_tag="JN692_001045"
     CDS             19008..19988
                     /locus_tag="JN692_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7263414.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWY
                     KQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAG
                     EGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECND
                     ATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(20058..20540)
                     /locus_tag="JN692_001046"
     CDS             complement(20058..20540)
                     /locus_tag="JN692_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7263415.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            20775..23357
                     /gene="leuS"
                     /locus_tag="JN692_001047"
     CDS             20775..23357
                     /gene="leuS"
                     /locus_tag="JN692_001047"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7263416.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23372..23953
                     /gene="lptE"
                     /locus_tag="JN692_001048"
     CDS             23372..23953
                     /gene="lptE"
                     /locus_tag="JN692_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7263417.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPINAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23953..24984
                     /gene="holA"
                     /locus_tag="JN692_001049"
     CDS             23953..24984
                     /gene="holA"
                     /locus_tag="JN692_001049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7263418.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24986..25627
                     /gene="nadD"
                     /locus_tag="JN692_001050"
     CDS             24986..25627
                     /gene="nadD"
                     /locus_tag="JN692_001050"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7263419.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25651..26262
                     /locus_tag="JN692_001051"
                     /pseudo
     CDS             25651..26262
                     /locus_tag="JN692_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            26522..26839
                     /gene="rsfS"
                     /locus_tag="JN692_001052"
                     /gene_synonym="rsfA"
     CDS             26522..26839
                     /gene="rsfS"
                     /locus_tag="JN692_001052"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7263420.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26843..27310
                     /gene="rlmH"
                     /locus_tag="JN692_001053"
     CDS             26843..27310
                     /gene="rlmH"
                     /locus_tag="JN692_001053"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7263421.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27341..29242
                     /gene="mrdA"
                     /locus_tag="JN692_001054"
     CDS             27341..29242
                     /gene="mrdA"
                     /locus_tag="JN692_001054"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7263422.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKIDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKQWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29245..30357
                     /gene="mrdB"
                     /locus_tag="JN692_001055"
                     /gene_synonym="rodA"
     CDS             29245..30357
                     /gene="mrdB"
                     /locus_tag="JN692_001055"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7263423.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30368..31456
                     /gene="rlpA"
                     /locus_tag="JN692_001056"
     CDS             30368..31456
                     /gene="rlpA"
                     /locus_tag="JN692_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7263424.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31595..32806
                     /gene="dacA"
                     /locus_tag="JN692_001057"
     CDS             31595..32806
                     /gene="dacA"
                     /locus_tag="JN692_001057"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7263425.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALDLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32917..33180
                     /locus_tag="JN692_001058"
     CDS             32917..33180
                     /locus_tag="JN692_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7263426.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33281..33922
                     /gene="lipB"
                     /locus_tag="JN692_001059"
     CDS             33281..33922
                     /gene="lipB"
                     /locus_tag="JN692_001059"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7263427.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34181..35134
                     /locus_tag="JN692_001060"
     CDS             34181..35134
                     /locus_tag="JN692_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7263428.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPNPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35343..36308
                     /gene="lipA"
                     /locus_tag="JN692_001061"
     CDS             35343..36308
                     /gene="lipA"
                     /locus_tag="JN692_001061"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7263429.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36409..36612)
                     /gene="tatE"
                     /locus_tag="JN692_001062"
     CDS             complement(36409..36612)
                     /gene="tatE"
                     /locus_tag="JN692_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7263430.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36741..37529)
                     /locus_tag="JN692_001063"
     CDS             complement(36741..37529)
                     /locus_tag="JN692_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7263431.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            37622..38005
                     /gene="crcB"
                     /locus_tag="JN692_001064"
     CDS             37622..38005
                     /gene="crcB"
                     /locus_tag="JN692_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7263432.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(38059..38268)
                     /gene="cspE"
                     /locus_tag="JN692_001065"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38059..38268)
                     /gene="cspE"
                     /locus_tag="JN692_001065"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7263433.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38443..39003)
                     /locus_tag="JN692_001066"
     CDS             complement(38443..39003)
                     /locus_tag="JN692_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7263434.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39590..40975
                     /gene="dcuC"
                     /locus_tag="JN692_001067"
     CDS             39590..40975
                     /gene="dcuC"
                     /locus_tag="JN692_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7263435.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHNVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(41016..41357)
                     /locus_tag="JN692_001068"
                     /pseudo
     CDS             complement(41016..41357)
                     /locus_tag="JN692_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT        10425 a        10478 c        10356 g        10287 t
ORIGIN      
        1 aagaaataat ctggctacgt agctcagttg gttagagcac atcactcata atgatggggg
       61 cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac gttcggcaaa
      121 ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc tgattccggc
      181 attccgaggt tcaaatcctc gtaccccagc caatttattc aagacgctta catcgtaagt
      241 gcacccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
      301 gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt
      361 tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc
      421 gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt tgtaaggcta
      481 caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat
      541 taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa
      601 cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt
      661 gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca gaacatcaat
      721 cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt
      781 atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg
      841 cgtcagcgga aacgcaccag cggcaagcgg tataacgtaa cccagacgcg acgggaaatg
      901 cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc gaatacgcgc
      961 cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgcgg
     1021 tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct
     1081 aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg
     1141 cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg tttcaccgcc
     1201 cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg aggttggcac
     1261 acgtaacgtt actttcggat gcgcttccag cgcctgccac aacgcctgtt gcaggactgt
     1321 gttttccacc atgtagccaa gcaacggcaa ctttaattcg gctgcgtcaa ataccacatg
     1381 tgccgtttcc cactcccagg tttccagtct gcggtaagga tggcaacgca tagcctgtac
     1441 tgcatcccag acccctaacc ctttaagcaa tgataccgaa gccgcgctga tcgccgagat
     1501 ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt
     1561 taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca ttcctccgcc
     1621 gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct
     1681 ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt cacaacggca
     1741 gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat
     1801 gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa
     1861 gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg cggaagaagc
     1921 ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca
     1981 tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg
     2041 tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat
     2101 tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga
     2161 ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctgccgga
     2221 accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg
     2281 cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat
     2341 tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca
     2401 gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc accggatcgt ttgccgatct
     2461 gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc
     2521 gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc tggtgagctt
     2581 cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg gacgtaccca
     2641 tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cggatattca
     2701 gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact tcgagaaaac
     2761 gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg
     2821 tccaggaacg cctgccgccg atatggttga tgatgttccg gaagaagaga agaagcagcg
     2881 tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gtcggatgct
     2941 cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc
     3001 cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa
     3061 attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg
     3121 aactgaagat gaaatgggtc tgcgcatggc agaaacaccg gagtcggtga ttgcccgcac
     3181 ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag gcctgccgtt
     3241 ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt
     3301 tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg gcggcataca
     3361 gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaat
     3421 gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt
     3481 ctcggcatcg agatcaatcg ccgcgataac cactttaaac tgaccggccg tccgatttgc
     3541 gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atactgcccc gatgcgcggt
     3601 cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc gcgggtactg
     3661 gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aactaaacgc
     3721 ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca ttgccaatat tctcgaccat
     3781 gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctaccaggc agtggctgcg
     3841 gcagttgatg ccctggagcg tcaggaaatt cgccgtattc tgctgactcg tccggcggtc
     3901 gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat
     3961 ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt cgagaaactg
     4021 attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac
     4081 gacgcgttta tcattctcga tgagagccag aacactacca tcgaacagat gaagatgttc
     4141 ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac
     4201 ctgccgcgta atactaaatc aggcttacgt catgccattg aagtgctggc cgatgtcgaa
     4261 gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcacccggt ggtggcgcgt
     4321 atcgttaacg cttatgaagc ctgggaagaa gcagaacaaa aacgtaaagc ggcgctggca
     4381 gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac
     4441 tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg
     4501 cggtgatccc gcagtttcag gaagagtcgg aagtgactat tcgcgtggtc gataccgccg
     4561 aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct
     4621 tcccgtttga agtgccgcct ggcatggaga tgtcgctact gggcgatctg gttatctgcc
     4681 gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc
     4741 atatggtggt gcacggcagt ctgcatttgt taggttacga tcacattgaa gatgacgaag
     4801 cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt
     4861 acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt
     4921 gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga
     4981 caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag
     5041 ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc
     5101 cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat
     5161 cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg
     5221 caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc
     5281 ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc
     5341 gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt
     5401 cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca
     5461 catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat
     5521 cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg atatcgactt
     5581 ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg aagacttcaa
     5641 cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg gtctggtgat
     5701 gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt
     5761 caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa tcccggatga
     5821 ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact acatggcttt
     5881 tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg
     5941 cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt cgctgatggg
     6001 gcttcaggcg ttgaccttta accgtcgccc actccagtct gccgctattg gcttttgctg
     6061 gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg
     6121 cgggatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct
     6181 gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg
     6241 cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg gttgggtgct
     6301 gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct
     6361 ggcaccgcta atgggcgtgg aagccattaa cttcctgctg atgatggtta gtggcctgct
     6421 ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc
     6481 cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca
     6541 ggtttcgatg gttcagggcg atattccgca atcgctgaaa tgggacgaag gccagcttct
     6601 taatacgcta aagatttact acaacgcaac ggcaccgctg atgggcaaat catcgttgat
     6661 tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc
     6721 actggacggt gagttgcgtg ataaaggtag ctcgctggta accgggattg tcgacgcgcg
     6781 tctcaataag cagaaccgct acgataccta caacaccatc atcacgctgg gtaaaggtgc
     6841 accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc cgtttggcga
     6901 gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc
     6961 gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gcaaatggta ttgagcttac
     7021 tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact tccgcccgga
     7081 taccgactac ctgctgacta tctccaacga tgcgtggttt ggtaaatcta ttggtccatg
     7141 gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag
     7201 caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg cgatgatccc
     7261 gcagttcacc cgcgaggtgt taaccactaa cgtgacgccg accaccggac tcacaccata
     7321 cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt ttgctgctgt
     7381 gttgatgagt ctgcgtcagc gacgtaaata atcccttctt atcgtgccgg aatacatccc
     7441 atatttcggc acgctcaccg gatgcggcat cagtgcatta tccggataac aatattctcc
     7501 tatccaactt tggcacatct attgctttgt tatacaaggc aaagcttgaa ccagcatcag
     7561 tacaacccgg ttgcaccaca gcaatccacc ggtagcacca aaagagtgca ttttaagaat
     7621 tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac aataaaacat caatatcttt
     7681 acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa agttgcagac
     7741 gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga agataaagga
     7801 gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc tttccgcagg
     7861 actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca aaatcgccaa
     7921 aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt attacgacaa
     7981 tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg aagcagtgaa
     8041 aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct cacaaaaccg
     8101 tattccactg ctgcaaaacg gcactttcga ttttgaatgt ggttctacca ccaacaacgt
     8161 cgaacgccaa aaacaggcgg ctttctctga cactattttc gtggtcggta cgcgcctgtt
     8221 gaccaaaaag ggcggcgata tcaaagattt tgccgacctg aaaggcaaag ccgtagtcgt
     8281 cacttccggc actacctctg aagttttgct caacaaactg aatgaagagc aaaaaatgaa
     8341 tatgcgcatc atcagcgcca aagatcacgg tgactctttc cgcaccctgg aaagcggtcg
     8401 tgccgttgcc tttatgatgg atgacgctct gctggccggt gaacgtgcga aagcgaagaa
     8461 accagacaac tgggatatcg tcggcaagcc gcagtctcag gaagcctacg gttgcatgtt
     8521 gcgtaaagac gatccgcagt tcaaaaagct gatggatgac actatcgctc aggtgcagac
     8581 ctccggtgaa gcggaaaaat ggtttgataa atggttcaaa aatccaattc cgccgaaaaa
     8641 cctgaacatg aatttcgaac tgtcagacga aatgaaagca ctgttcaaag aaccgaatga
     8701 caaggcactg aactaattac aagaaccagg ggcggaaatt ccagccctct cgattgttac
     8761 gtagcacgga cagactatac gcctgatggt cgttccccat cgggcctgaa aaccgcaata
     8821 cgctgggtaa caatcttcga gggtagcagt taacgctgct accctctttt ttctggagta
     8881 gatttatgtc tatagactgg aactggggta tttttttaca acaagccccg ttcggcaata
     8941 ccacctatct tggttgggtc tggagtggtt ttcaggtgac gatcgctttg tcgatctgcg
     9001 cctggattat cgctttcctc gtcggttcat ttttcggcat tttacgtacc gttccaaacc
     9061 gttttctctc cggtctgggt acgttgtatg tcgaactgtt ccgcaacgtg ccgttgattg
     9121 tgcaattctt tacctggtat ctggtgatcc cggagctgct gccggagaaa atcggcatgt
     9181 ggtttaaggc cgagctggat cccaatattc agtttttcct ttcatccatg ctctgcctgg
     9241 ggctgtttac cgccgcccgt gtttgcgaac aggtacgcgc cgctattcag tcgctaccgc
     9301 gtgggcagaa gaatgccgcg ctggcgatgg gcctgacgct accgcagact tatcgatacg
     9361 tactgctgcc taatgcttat cgcgttatcg tcccgccgat gacttccgag atgatgaacc
     9421 tggtgaaaaa ctccgccatc gcctcgacca tcggtctggt ggatatggcg gcgcaggcgg
     9481 gtaaattgct cgactactcg gctcacgcct gggagtcgtt taccgccatc acgcttgcgt
     9541 atgtgttgat taacgctttt atcatgctgg tgatgacgct ggttgaacgt aaagttcgcc
     9601 tgcccggcaa catggggggc aaataatgta cgagtttgac tggagttcca ttgttccttc
     9661 cctgccatat ctgctcgacg ggctggtgat caccctgaaa atcaccgtca cggcagtcgt
     9721 gattggtatt ttgtggggta ccatgctggc ggtgatgcgc ttgtccagct tcgcgcctgt
     9781 ttcgtggttt gccaaagcct acgttaacgt attccgctcg attcctttag tcatggtttt
     9841 gctgtggttt tacttgattg tgccgggatt tctgcaaaat gtgctgggat tatcgccaaa
     9901 aaatgatatt cgtttaattt cggcgatggt ggcgttttcg atgtttgaag cggcctacta
     9961 ttcagagatt atccgtgccg gtattcaaag tatttcccgt ggtcagtcga gcgccgcgct
    10021 ggcgttgggg atgactcact ggcagtcgat gaaactgatt attctgccgc aggctttccg
    10081 cgcgatggtg ccgctgctgc tcactcaggg catcgtactg ttccaggata cctcgctggt
    10141 gtatgtgtta tctctggccg atttcttccg taccgcctca accattggtg agcgtgatgg
    10201 tacgcaggtc gagatgatcc tgtttgccgg atttgtttat ttcgttatta gccttagcgc
    10261 gtcgttgttg gtcagctact tgaaaagaag gacagcataa tgattaccct gaaaaatgtt
    10321 tcaaaatggt atggtcactt tcaggtgctg accgactgct caaccgaagt gaaaaaaggc
    10381 gaagtggtgg tggtttgcgg cccgtctggt tccggcaaat caacgctgat taaaaccgtc
    10441 aacggcctcg aaccagtgca gcaaggtgaa atcaccgtcg atggtatcgt ggttaacgac
    10501 aagaaaaccg atctggcaaa gctgcgttcc cgcgtcggga tggtattcca gcatttcgag
    10561 ctgttccctc atctgtcgat tatcgaaaac ctgaccctgg cgcaggtgaa agtgcttaaa
    10621 cgcgataaag cgcccgctcg tgaaaaagca ttgaaattgc tagagcgcgt gggactttcc
    10681 gcacatgcca ataaatttcc ggcgcagctt tccggcggtc agcagcagcg tgtggcaatc
    10741 gctcgcgcat tgtgtatgga tcctattgcg atgctgttcg acgaaccgac atcggcgctg
    10801 gatccggaga tgatcaacga agtgctggac gtgatggtgg aactggcgaa cgaaggaatg
    10861 accatgatgg tggtgaccca cgaaatgggc tttgcccgca aagtggcgaa tcgggtgatc
    10921 tttatggacg agggtaaaat tgtcgaagac tcgccgaaag acgctttctt cgatgatccg
    10981 aaatcggacc gcgcaaaaga cttcctcgcg aaaatcctgc attaatcact ctggcgcgca
    11041 tacccaattg cgcgccatct cacgctttat cgtttcgccc ctcgtatctg ttacatcctg
    11101 gcgttaccct tgtcgcaaag aagcacacaa caaggagcaa caatggcact gccaattctg
    11161 ttagattgcg acccaggtca tgatgacgct atcgcaatag ttctcgccct cgcctcacca
    11221 gagcttgatg tcaaagcaat tacgtcttcc gccggaaacc agacaccaga aaaaacctta
    11281 cgcaatgttc tgcgtatgct gaccttgctt aatcgcaccg atattccggt ggcaggcggc
    11341 gcggtaaaac cgttaatgcg tgagttgatt atcgcggaca atgtgcacgg cgaaagcggt
    11401 ctcgacggcc cggcattacc ggaaccgacg ttcgcaccgc aaaactgtac ggcggtggag
    11461 ctgatggcga aaacgctgcg cgaaagtgcg gaacctgtca cgattgtttc taccggaccg
    11521 caaactaacg ttgccttgct gctcaatagc catccggaac tgcatagcaa aattgcccgt
    11581 atcgtgatta tgggcggcgc aatggggctt ggtaactgga cgcctgcggc tgaatttaat
    11641 atttacgttg acccggaagc ggcagaaatt gtcttccagt cagggatccc ggtggtgatg
    11701 gccggtctgg atgttactca taaagcgcaa atccacgttg aagacaccga gcgtttccgc
    11761 gcgattggca accctgtttc aaccattgtt gccgaactgc tggatttctt cctcgaatat
    11821 cataaagacg aaaaatgggg ctttgtcggc gcaccgctgc atgacccatg caccatcgcc
    11881 tggctgttga aacccgagtt gtttaccact gttgagcgct gggttggcgt ggaaacacag
    11941 gggaaatata cccagggtat gacggttgtt gattattatt atctgactgg caataaaccg
    12001 aatgccactg taattgtcga tgttgatcgt cagggctttg ttgatttact ggctgatcgt
    12061 ctgaaatttt acgcttaaca ctcgtcattg attaagatca ccacgcgtta cctgggtggt
    12121 cttaatctat ttatctcttt ttctttcaag atataaatgc aatggataat gcagaactcg
    12181 ccataggtat cgatctcggc actaccaata gtttaattgc cgtctggaaa gacggtgccg
    12241 cgcaattaat tccaaataag ttcggtgaat atttaacacc atccataatt agcatggatg
    12301 aaaataatca tattttagtc ggaaaaccgg ctgtatcacg gcgtacttcg catccggata
    12361 aaacggcagc gttatttaaa cgtgcaatgg gcagtaatac caactggcgg ttaggcagcg
    12421 acacatttaa cgcgccagaa ctgtcctctt tggtattacg ctcattaaaa gaagatgccg
    12481 aagaatttct gcaacgtccg attaaagatg tggtgatctc cgttccggct tatttcagcg
    12541 atgaacaacg caagcatacc cgtttagcag cggacttagc cgggttaaat gcggtacgct
    12601 taattaatga acccacagca gctgcgatgg cgtatggcct gcatacccaa caaaataccc
    12661 gttcgctggt gtttgatctc ggtggcggca cgtttgacgt tacggtgctt gagtacgcca
    12721 cgccggtgat tgaagttcac gcctccgctg gcgacaactt cctcggtggc gaagatttta
    12781 cccatatgtt ggtcgatgac gttttaaagc gcgcggatgt cacaaggacc acgcttaacg
    12841 agagtgaact ggcagccttg tacgcctgtg tggaagcggc aaaatgtagc aatcaatcgc
    12901 caatgcacat tcgctggcag taccaggatg aaacgcggga atgcgaattt tacgagaacg
    12961 aactggaaga tttgtggttg ccgctgctca atcgcttgcg agtgccgatt gaacaggcgt
    13021 tgcgcgatgc gcgtctgaag ccgagtcaaa tcgacagtct ggtgctggtt ggcggcgcgt
    13081 cacaaatgcc gctggtgcag cgaatcgccg tgcgtctgtt tggcaaatta ccgtatcaaa
    13141 gttacgatcc gagcaccatt gtcgcgctgg gcgcagcaat ccaggccgcc tgccgcttac
    13201 gcagtgaaga tattgaagaa gtgatcctca ctgatatttg cccttactcg ttgggcgttg
    13261 aggttaaccg ccagggcgtt tccggcattt tctcgccgat tattgaacga aacaccactg
    13321 tgcccgtgtc gcgtgtagaa acttattcaa ccatgcaccc ggaacaggat tcaattacgg
    13381 ttaacgtcta tcagggagaa aaccacaaag ttaaaaacaa cattctggtg gaatccttcg
    13441 atgtgccgtt gaagaaaacc ggggcttatc agtcgattga tattcgcttt agttatgata
    13501 tcaacgggtt gcttgaagtt gacgtgcttc tggaagacgg cagcgttaag tccagagtga
    13561 ttaaccacag cccggtaaca ttgagcgcgc agcagattga agagagtcgg acgcggttat
    13621 ccgcattgaa aatttatccg cgcgatatgc tcatcaatcg cacctttaaa gccaaactgg
    13681 aagagttgtg ggcgcgggcg ctgggtgacg agcgagaaga gattggccgg gtgatcaccg
    13741 attttgatgc ggcgctgcag tcaaacgata tggcccgcgt cgatgaggtc aggcggcggg
    13801 cgagcgttta tttagccatt gagacctcat aatcacggct gccggatgcg gcgtaaatgc
    13861 tgcatccggc atcggcgcaa aaaacacatc gtcaactaaa caacatcgcg attttgccga
    13921 gagcataacc tataaccagc accgttaaac tgattttgat aatgctggta atgctctgtg
    13981 gtttttcacc ctcatcattt ttttcttcat ggttttcagg tgttggctca tcgacatctt
    14041 catcctccgg ctcgagtttt actttcgtca acagcatccg ggtgataatg gcctctaatg
    14101 gatgagcatc tccgccttcc agccttgtat taagacgggc cagcatctct tgtttcggaa
    14161 acgcttcaag tactacctgt tccgctaccc gatacaaatc cagtaacggt gaatcttctt
    14221 ctgtctcgct accgactctc gatttccacc acgcccatgc tttgcgtttg aagaataacg
    14281 actcaataaa cgattgacct aacggcgata attttccgct atgtaaaatt tccgcccata
    14341 acagccgcgt ccgggcgctc tcaccaggaa catatttcag cggttgacct tgcgcatcta
    14401 ccatgctccg cgcttcgata accatcacta ataacgcgaa ataatccggg caatgacgct
    14461 tgctccactg caacaacaaa ttatcagctt gtgtagatgg gtaactttcc cagtacgcgc
    14521 cgagatcggc aagcaggctt tccccctcgc cgcagtacaa acgttgtgca cactggtaat
    14581 accgggcatc ttcgctttct ggttcttccg cttgccactg tttggctaca ggaatgagtt
    14641 ccaccatacc tgtttgcacc gagctgtacc agcgcaacag tttgcgatgt aactttgcat
    14701 catcaggaat gaaccacggt ccttccatcg ccaaaaacgc agtgacatag tcaggatggt
    14761 aacggcagca acgttccagc gcgaagtaat attcaacagt ctggttctgt aacgcgacgg
    14821 gatattccag aaggctaagg aaatcgaaac aatcaccagc ctgaatggcc tcaagaaaag
    14881 cctccctttc ttcctcatcc attccttcgc tgtcattaaa cgaatgccag ttcaaacgct
    14941 ctgccaacaa agaagcacaa tttaatgaca gatatcgcgc ttctatagcg attgcacaca
    15001 gcggccagcg taattgatcg acatcctcca tgttccaggt atttaattgc tgaataaatt
    15061 tctgccaggc ggagggctga aaacgatcac tttctgaatc cagtaatgtc ctgaatgcac
    15121 gtagtatttc atgttcagcg gtggcatctt tttcttcatc atctgcttcc tcgacaggat
    15181 ttaccgccag tcgcagcgcc tcttcatagg cctggcgtaa tgctttaaac ccctgcggat
    15241 cggtttcggg atgacacaac ggtaagcgag caagataagc ctggcggata atgtctattt
    15301 gcgtcgtgct ttcaatttcg aggatttgcc aacatgtttt cataggtcgc tcacatcctt
    15361 aagcgttacc gggcattcag gctggacagt taatggctgc cagggaagcg tggcatagaa
    15421 acggtcgctt cccgctgcca gctcttcgaa catccgactc ccggcgctat cgtatttgta
    15481 cagggttagc gcctcgcgta gcgaagcgtt gtcttcgcac tgagaagatt gccagtagag
    15541 acgccctagt gaataggcag aaaaatagtg catccagctg tgatagtaat ggtgagctcg
    15601 tgcatagacg cgcgattgca gtcataaact ctcttcttcc gtcagccagt tgttgagcac
    15661 accgacccgt gacagaaacc ccattcgaac ataatcccag gctttgattc ccccttctcc
    15721 acagcacata gcggtttggg caacgaattg ggtataaatc aaccctctct ccgagccacc
    15781 agcaattaat gctttccact cttcagggga agagcgttgc catgtcagat ataagccagc
    15841 taaatcattg gcatgaccat catcagtcat ctgataaata gtctgaatta aatcatcacg
    15901 attatttatt ccccaggatg atgagagatc gaattctttt atcccggtat acatttttgg
    15961 cgagtcatgc gcgggtttgc aactctgatt aagtatttcc atgggcgcag aaagcgcaaa
    16021 aagaagatat tgttcttcct tattcatttt ttaataatac cttaagtgca ttaactgact
    16081 acttttttat tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagc
    16141 attcacacat gaataaaaaa ttaatgtata tattcgcaat ttttatagtt gcagcaatta
    16201 cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg aattatgcct
    16261 ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg
    16321 cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg
    16381 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcagaacagg
    16441 aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac
    16501 ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
    16561 ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg
    16621 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
    16681 ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag tattttccct
    16741 atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag acagcccagc
    16801 ccgccatcgt caacgggcat tggctgcggt tgcggctccg gctcgacggt ttcctgaccg
    16861 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
    16921 tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg ttggctggcc
    16981 tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg
    17041 agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccagc
    17101 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
    17161 ccggggacat aaagtagcgg ttttccctga tcgttaacca gatcacgcgc ttcaatcatc
    17221 atcaccacta atgggtaata atcgacccga tgctgacggc accattgcaa cattaaagca
    17281 tccgcctggg tggagggata ctcgcgccag tagtaacaaa gttctgccag caggctttcg
    17341 ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcaggt
    17401 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg ggattcccag gcggcttgcg
    17461 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
    17521 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt
    17581 tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag
    17641 ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
    17701 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
    17761 aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc
    17821 tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
    17881 cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg ttctgctacc
    17941 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc
    18001 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
    18061 aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatatcgagg
    18121 atcttccagc aatttttcat aagtcgctca tatccttaag cgtttccggg catgtggggt
    18181 agtcagccag tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt
    18241 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata
    18301 aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
    18361 agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc gactggatcc
    18421 atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt aaaaaaccca
    18481 tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct gtgctggcga
    18541 caaattgcgc ataagcctga ccttgctcat ttaattcagc aagatagtca cgccattcac
    18601 atggagaata acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttct
    18661 cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc catgaggaag
    18721 aaaggtcaaa aaagcatgta cctaaataca ttttggggga taaatgcgaa ggtaaacagc
    18781 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gaccccatgt
    18841 ccatataatt tatccttaaa cattcattta atgaatgtaa actacatttc gagactcagt
    18901 tatttatact tttctgacgc aacataatac gcataaaata aggcttacgc aaaaatggaa
    18961 cacataaaaa tataatatac tttatctaca accataagga aatgataatg atttttacgt
    19021 caagttgctg cgataattta tcaatagatg agattatcga acgtgctgaa aaaggcgatt
    19081 gcgaggctca atatattgtt gggttttatt ataatcgcga tagcgcaatt gactctccgg
    19141 acgacgaaaa ggccttttac tggctgaagc tggccgctga gcaaggtcat tgtgaagcac
    19201 agtattcctt agggcaaaag tataccgagg ataaaagccg tcataaagat aatgagcaag
    19261 ccatcttctg gctgaaaaaa gctgccctac aaggccatac tttcgcttcc aacgcccttg
    19321 gctggatact ggatcgtgga gaagacccca actataaaaa agaagcagtt acctggtatc
    19381 agatagccgc ggagagcgga atgtcttatg cgcaaaataa tcttggatgg atgtacagaa
    19441 atggcaacgg agtcgcacaa gactatacgc tggcattttt ctggtacaaa caagctgcat
    19501 tacaaggcca tagttacgca caagacaatc tggccgatct ttataaagac ggagaaggcg
    19561 ttgctcaaaa caagacactc gccgcattct ggtatttgaa aagcgcacag cagggtaatc
    19621 ggcacgccca gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg gaccaggact
    19681 ataagcaagc gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc ggcgcttacg
    19741 tcaacatcgg ttatatgtat aaacacggac aaggcgttga gaaagattat caggctgcct
    19801 ttgaatggtt tacgaaagcc gctgaatgca atgacgccac tgcctggtat aacctggcca
    19861 ttatgtatca ctacggagaa ggaagacctg tcgatctccg acaggctctc gacctgtatc
    19921 gtaaagttca gtcatccgga accagagatg tcagtcagga aattcgtgag actgaagatt
    19981 tactgtagaa ataaaaacaa gggccgggga atttcattct gcccatattc tctttattgc
    20041 tgtgcgaaat cagactatta cggctcaaac gggcgtctct ggaactggtc ataaccacat
    20101 ttcgggcata gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg
    20161 cagaccagat ttcccagccc gaccacttct ccgctgtgat aaaccccatg atgattgagg
    20221 tcctggaaaa cttcgcgcca ttcaagctgc gttttatcgg tgatgtctgc cagctcctgc
    20281 cacaagcttt ctttaatcac ccgcataaag acgctgtcag attcttcttt caggctctct
    20341 tcatagctca tggcgaactc ttccaggtca cgtctgacag ctcgcgtcag ctcatcgatc
    20401 tcggttcgcg ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg
    20461 tcgatatcac gttcgccatt gcgcaggcgt tcgttcagtg acgcgaccag ttcacggtaa
    20521 tattgagcaa ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga
    20581 cgttctttgt tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtacgaata
    20641 atccgggcat catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg acgtttgggc
    20701 tatgctatgc ggatctgaaa aaccacctca acgctacatt tgtagccgta ttgaaaacag
    20761 gaccactggc tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc
    20821 ttcattggga tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt
    20881 actgcctgtc tatgcttccc tatccttctg gtcgactaca catgggccac gtacgtaact
    20941 acaccatcgg tgacgtgatc gcccgttacc agcgcatgct gggcaaaaac gttctgcagc
    21001 cgatcggctg ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg
    21061 ctccggcacc gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg
    21121 gctttggtta tgactggagc cgcgagctgg caacctgtac gccggaatac taccgttggg
    21181 aacagaaatt cttcaccgag ctgtataaaa aaggcctggt atataaaaag acttctgcgg
    21241 tcaactggtg cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct
    21301 gctggcgctg cgataccaaa gttgaacgta aagagatccc gcagtggttt atcaaaatca
    21361 ctgcttacgc tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg
    21421 ttaaaaccat gcagcgtaac tggatcggtc gttccgaagg cgtggagatc accttcaacg
    21481 ttaacgacta tgacaacacg ctgaccgttt acactacccg cccggacacc tttatgggtt
    21541 gtacctacct ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg gaaaataatc
    21601 ctgaactggc ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg
    21661 cgacgatgga gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca ttaacgggcg
    21721 aagaaattcc cgtttgggca gcaaacttcg tattgatgga gtacggcacg ggcgcagtta
    21781 tggcggttcc gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca
    21841 tcaaaccggt tatcctagcg gctgacggct ctgagccaga tctttctcag caagccctga
    21901 ctgaaaaagg cgtgctgttc aactctggcg agttcaacgg tcttgaccat gaagcggcct
    21961 tcaacgccat cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc
    22021 gcctgcgcga ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt ccgatggtga
    22081 cgctggaaga cggtaccgta atgccaaccc cggacgacca gctgccggtg atcctgccgg
    22141 aagatgtggt gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa
    22201 ctaccgttaa cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc tttatggagt
    22261 cctcctggta ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg ctggattccg
    22321 aagcggctaa ctactggctg ccggtggata tctacattgg tggtatcgaa cacgccatta
    22381 tgcacctgct ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact
    22441 ctgacgaacc agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact
    22501 atgttggcga aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg
    22561 acgagaaagg ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg
    22621 gcatgagcaa aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac
    22681 gttacggcgc ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc
    22741 tcgaatggca ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac
    22801 tggtgtacga gcacacagca aaaggtgatg ttgcggcact gaacgttgat gcgctgactg
    22861 aagatcagaa agcgctgcgt cgcgatgtgc ataaaactat cgctaaagtg accgatgata
    22921 tcggccgtcg tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac
    22981 tggcgaaagc accgaccgat ggcgagcagg atcgcgctct gatgcaggaa gcactgctgg
    23041 ccgttgtccg tatgctcaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac
    23101 tcaaaggcga aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg
    23161 tggaagactc cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc aaaatcaccg
    23221 ttccggtgga cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag
    23281 caaaatatct tgatggcgtt actgtacgta aagtgattta cgtaccaggt aaactcctca
    23341 atctggtcgt tggctaagcg cgggaggaag cgtgcgatat ctggcaacat tgttgttatc
    23401 tctggcggtg ttaatcaccg ccgggtgtgg ctggcatctg cgtgacacca cgcaggttcc
    23461 ttccactatg aaggtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc
    23521 ggtgcgtaac cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa
    23581 ggacgttcca tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt
    23641 ccgtaacggt caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat
    23701 ccccggccgt gatatctacc cgattaacgc caaagtcttc cgttcattct tcgataaccc
    23761 gcaaatggcg ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg
    23821 tgctgccgaa cagctgattc gtaagctgcc aagcatccgt gctgcggata ttcgttccga
    23881 cgaagaacag acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccac
    23941 gctgggtaac tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg
    24001 ctgcgcgcgg cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac
    24061 gctgttcgcc aggtagctgc ggcacaagga ttcgaagaac accacacttt ttccattgat
    24121 cccaacactg actggaatgc gatcttttcg ttatgccagg ctatgagtct gtttgccagt
    24181 cgacaaacgc tattgctgtt gttaccagaa aacggaccga atgcggcgat caatgaacaa
    24241 cttctcacac tcaccggact tctgcatgac gacctgctgt tgatcgtccg cggtaataaa
    24301 ttaagcaaag cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag
    24361 gtgacctgtc agacaccgga gcaggctcag cttccccgct gggttgctgt gcgcgcaaaa
    24421 cagctcaact tagaactgga tgacgcggcg aatcaggtgc tctgctactg ttatgaaggt
    24481 aacctgctgg cgctggctca ggcactggag cgtttatcgc tgctctggcc agacggcaaa
    24541 ctgacattac cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat
    24601 tgggttgatg ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct tcagcaactg
    24661 cgtctggaag gcagcgagcc ggttattttg ttgcgcacat tacaacgtga actgttgtta
    24721 ctggttaacc tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat
    24781 cgggtatggc agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt aagtcagccg
    24841 cagttacgtc aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac
    24901 ggtcagtcag tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca taaacccctg
    24961 gcggacgtat ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt
    25021 tgatccggtg cactatggtc atctaaaacc cgtggaaacg ctggcgaatt tgattggtct
    25081 gacgcgggtc acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa
    25141 cagcgtgcag cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct
    25201 tgatgaacgc gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg
    25261 gcggcaggaa caaggaccgg acgtgccgct ggcgtttatt attggtcagg attcattgct
    25321 gacctttccg acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg
    25381 tcggcgtcca ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga
    25441 tcatttgaca cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc
    25501 tgaaacgccg tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga
    25561 atcgtgtgag gatttattgc cggaaccggt attgacttac attaaccaac aaggcttgta
    25621 tcgctgatac ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggcgaa
    25681 acacaagcga atgtcgatgg tctgtacagc ggtcatgcgc ccacacccct gaccgcacgc
    25741 ggtattgagc aagcgcaaaa tctgcataca ctactagatg atgtttcctt tgacctggtt
    25801 ttatgcagtg aactggaacg ggcacagcat accgcgcgac tggttctcag tgaccgccag
    25861 ctccccgttc atatcatccc tgaactcaac gaaatgtttt ttggcgactg ggagatgcga
    25921 catcatcgcg acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg
    25981 cagcatgcta tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaacgc
    26041 tttatcgcaa ggcttagtga atatcagcac tatcagaata ttttaatcgt cagccatcag
    26101 ggcgtactga gtctgttaat tgctcgttta attggcatgc ctgccgagtc tatgtggcat
    26161 tttcgcgttg actaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta
    26221 cgcgtcctca atagccgtgc catcggggtc gagaatgcat gacttttctg tttttttacg
    26281 ggtaagccgc aacgaccatt gacagccccg ggcaggctga tattctccgc agccagactt
    26341 tttccgccag acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc
    26401 gcggggtggg atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg
    26461 cctctggttc aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa
    26521 cttgcagggt aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca
    26581 ggacatcatc gccttagacg ttcaaggcaa atccagcatc accgactgca tgatcatctg
    26641 tacgggtacg tccagccgtc atgttatgtc cattgctgac cacgttgtgc aggagtctcg
    26701 cgcagcgggc ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt
    26761 ggatttgggc gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact
    26821 ggaaaaactc tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc
    26881 ggactgggta caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt
    26941 cgagctgatt gaaattccgg ccggcaaacg cggcaagaat gcggacatca agcgcatact
    27001 cgacaaagag ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga
    27061 tattccaggc aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct
    27121 ggatggtcgc gacgtaagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa
    27181 agcggcggct gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc cgctggttcg
    27241 cgtgctggtc gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca
    27301 ccgtgagtga taagggagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt
    27361 tcgcgactat acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg
    27421 gattttgctg ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt
    27481 taccgactac cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag
    27541 ccgcggcatt atctacgacc gtaacggtat ccctctggcc ctcaaccgca ctatctacca
    27601 gatagaaatg atgccggaga aaatcgataa cgtgcagcaa acgctggacg ctttgcgcag
    27661 cgtggtagat ctgaccgatg acgatattgc tgcattccga aaagagcgcg cacgttcaca
    27721 ccgtttcacc tctattccgg tgaaaactaa cctgaccgaa gtacaagtag ctcgctttgc
    27781 cgtcaatcag taccgttttc cgggtgtcga agttaaaggc tataaacgtc gttactatcc
    27841 ttacggttcg gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt
    27901 cgaacgcctg aataatgacg gcaaactggc caactatgcg gcaacgcatg atattggtaa
    27961 gctgggcatt gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt
    28021 tgaagttaac aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaagccgg
    28081 acacgatatt tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc
    28141 gggtagccgc gcagctgtgg ttgttaccga tccgcgtaca ggtggggtgc tggcgctggt
    28201 ttccacgcct agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc
    28261 cgccttgctg aacgacccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc
    28321 tcccgcgtct acagttaaac cctatgtggc ggtttcggca ttaagcgccg gggtgatcac
    28381 gcgcaatacg acgctgtttg acccaggctg gtggcaacta ccagggtcgg aaaaacgtta
    28441 tcgtgactgg aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc
    28501 tgcggatacc ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg
    28561 gatgggtaaa ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg
    28621 caacatgcct acccgcgaat ggaaacagaa acgctttaaa aaacagtggt atcagggtga
    28681 caccattccg gttggtatcg gccagggtta ctggacagcg accccaatcc agatgagtaa
    28741 ggcactgatg atcctgatta atgacggtat cgtgaaggtt ccgcatttgc tgatgagcac
    28801 cgccgaagac ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg tcggcgatat
    28861 tcattccggt tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa
    28921 tggtacggcg cataaatact ttgccagcgc accgtacaaa attgcggcga aatctggtac
    28981 cgctcaggtc ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg
    29041 tttacgtgac cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt
    29101 cgccatgatt ctggagaacg gcggcgcggg tccggcggtt ggtacgctga tgcgccagat
    29161 cctcgaccac attatgctgg gtgataacaa caccgatctg cctgcggaaa atccagcggt
    29221 tgccgcagcg gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat
    29281 aaagtccatc tcgatcccac aatgctgctg attttactgg cattgctggt ttacagcgcc
    29341 ctggttatct ggagcgccag cggtcaggat attggcatga tggagcgtaa aatcggccaa
    29401 atcgcgatgg gtctggtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa
    29461 ggctgggccc cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc
    29521 ggagccatct ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg ttttcagccg
    29581 tcggaaattg ccaaaatagc cgtaccactg atggttgcgc gctttatcaa ccgcgacgtt
    29641 tgcccgccat cgttgaagaa cactggcatc gcgctggtgc tgatatttat gcccacgctg
    29701 ctggtggctg cacagcctga cctaggaaca tcaatcctcg ttgcgctttc cggtctgttt
    29761 gtactgttcc tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtagcg
    29821 gcgttcattc cgattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg
    29881 atgctcctgg acccggaatc agacccactc ggcgcgggct atcacattat tcagtctaaa
    29941 attgctattg gctccggcgg attacgcggc aaaggctggc tgcacggcac tcagtcacag
    30001 cttgaatttc tccccgaacg ccatactgac tttatcttcg cggtactggc ggaagagctg
    30061 ggattagtgg gcattctgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg
    30121 tggatagccg ccagagcgca aaccaccttt ggtcgcgtca tggctggtgg cttaatgctg
    30181 atattattcg tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta
    30241 ggggttccgc tcccactggt cagttatgga ggatcggcgc taattgtgct gatggctggg
    30301 ttcgggattg taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag
    30361 gtgcgcaatg cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg
    30421 tacaagcgat gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg
    30481 ccccatagtt gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg cgacggcaaa
    30541 tcaggattac cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag
    30601 ccaggcggga ctggcggcaa tctatgatgc cgaaccaggc agtaacctga cggcctctgg
    30661 cgaagctttc gatccgacaa agctgacggc ggcccatcca acgcttccga tccccagcta
    30721 cgccagaatc actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg
    30781 cccttacggc aacgaccgcg ttatttcgct ttctcgcgcg gcagctgacc gtcttaacac
    30841 gtcaaacaac accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc
    30901 tggtcctggt atggcttgta ccacagtcgc caaacagact tacgccctgc ctgcacctcc
    30961 cgatttaagc ggtggcgcgg gaacaagttc agtgtctggc ccgcagggtg acattcttcc
    31021 ggtcagtaat tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagtagcgg
    31081 tttcctcggc gctccaacga ccttagcgcc tggtgtactg gaaggcagcg aaccgacgcc
    31141 tgcgccacag cccgttgtta cagctccgtc gacaacgcct gcaacctcgc ctgcaatggt
    31201 gacaccgcaa gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt
    31261 aagcgatcag gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc
    31321 cggtcgcgta actcaaaatg gcgcggtctg gcggatccag cttggcccat tcgccagcaa
    31381 agccgaagcc agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat
    31441 tactaccgcg cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta
    31501 acaaagtcac gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt
    31561 ttttaattcc atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat
    31621 catgaagcgc ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc
    31681 cgatgacctg aatatcaaaa ctatgatccc gggtgtaccg cagatcgatg cggagtccta
    31741 catcctgatt gactataact ccggcaaagt gctcgccgaa cagaacgcag atgtccgccg
    31801 cgatcctgcc agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc
    31861 cggtaaattt aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa
    31921 cccggtgttt aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc
    31981 tcagctgatc cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga
    32041 ttttgccgct ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgcact
    32101 ggacctgaaa aatacccact tccagacggt acatggtctg gatgctgatg gtcagtacag
    32161 ctccgcgcga gatatggcgc tgatcggcca ggcattgatc cgtgacgtac cgaatgaata
    32221 ctcgatctat aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg
    32281 cctgttatgg gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc
    32341 aggttacaac cttgttgctt ctgcgactga aggccagatg cgcttgattt ctgcggtgat
    32401 gggtggacgt acttttaaag gccgtgaagc cgaaagtaaa aaactgctaa cctggggctt
    32461 ccgtttcttt gaaaccgtta acccactgaa agtaggtaaa gagttcgcct ctgaaccggt
    32521 ttggtttggt gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat
    32581 tccgcgtggt cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca
    32641 tgcgccgctg caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac
    32701 gatcgagcaa cgcccgctgg ttgtgttgca agaaatcccg gaaggtaact tcttcggcaa
    32761 aatcattgat tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg
    32821 aaagtgtaat ttccgtcccc atatactaag catcagtaaa aaaactcccg ccttctggcg
    32881 ggagttgcta tttaattacg ttacgccgga gctgacatga aaaccaaact taacgaactg
    32941 cttgaattcc ctactccttt tacttacaaa gttatggggc aggcgttacc tgagctggtt
    33001 gatcaggtgg ttgaagtggt acagcgccat gcgccaggtg actacacccc aacggtaaaa
    33061 ccaagcagca aaggcaacta ccactcggta tctatcacta tcaacgccac tcatatcgag
    33121 caggttgaaa cactgtatga agaactgggc aaaatcgata ttgtccgcat ggttctgtaa
    33181 ctcgcttctc cgttacccgg ctcctggtcg ggtaactccc ctctcctcgc tgtgatatac
    33241 ttttccccca cttttactca ttctccacgg agatgccgtt ttgtatcagg ataaaattct
    33301 tgtccgccag ctcggtcttc agccttacga gccaatctcc cgggctatgc atgaattcac
    33361 cgatacccgc gatgatagta cccttgatga aatctggctg gtcgagcact atccggtatt
    33421 cacccaaggt caggcaggaa aagcggagca cattttaatg ccgggtgata ttccggtgat
    33481 ccagagcgat cgcggtgggc aggtgactta tcacgggccg ggacaacagg tgatgtatgt
    33541 gttgcttaac ctgaaacgcc gtaaactcgg tgtgcgtgaa ctggtgacct tgcttgagca
    33601 aacagtggtg aataccctgg ctgaactggg tatagaagcg catcctcggg ctgacgcgcc
    33661 tggtgtctat gtcggggaaa agaaaatttg ctcactgggt ttacgaattc gacgcggttg
    33721 ttcattccac ggtctggcat taaacgtcaa tatggatctt tcaccatttt tacgtattaa
    33781 tccttgtggg tatgccggaa tggaaatggc taaaatatca caatggaaac ccgaagcgac
    33841 gactaataat attgctccac gtttactgga aaatatttta gcgctactaa acaatccgga
    33901 cttcgaatat attaccgctt aattccatac atcaatggcc taatttacac tgggtcatta
    33961 ctcataatac ctgctaatgc tttacatctc cttcgcgttt gtatattttc tttatcactt
    34021 gcagaaatgc ggtgccacgc cttgctccgt aactcacccc attgattaac ctattgaaat
    34081 agttgattat ggtgagcaaa agcgttccgt tccaattaga cataagatta ttgtaatttt
    34141 ttaaatatta tttttccatg aataaaattg gagtaagtgc gtggatagta ataatcaaat
    34201 tgaaccctgt ttaagcagaa agtcgtcaga aggtaaacca caaatattta caaccttacg
    34261 aaatattgat cttaaccttc tgactatttt tgaagctgta tatgtacata aagggatcgt
    34321 taatgcagcg aaagtgctta atctgacccc ctcggcaatc agtcagtcta ttcagaaact
    34381 gcgcgttata ttccctaacc cattgtttat tcgcaaaggc cagggtgtca ctcctaccgc
    34441 atttgcgatg catctacatg agtatatcag tcagggcctt gagtccattc ttggcgcgct
    34501 ggatatcgaa ggaagctatg ataagcaacg aacgataact attgcgacca ctccctcggt
    34561 cggagccctg gtccttcctg tcatctatcg ggcgattaaa actcactatc cgcagctttt
    34621 actgcgcaac ccgcccatca gcgacgcgga aaaccaactc agtcagtttc aaaccgatct
    34681 catcatcgat aacatgtttt gcaccaatcg tacggtgcaa catcatgttc tgttcaccga
    34741 caatatggtg ttaatttgcc gcgagggaaa tccactactc tctttagaag atgacagaga
    34801 gactatcgac aacgctgcgc atgtacttct gttaccggaa gggcaaaatt tcagcggtct
    34861 gcggcagaga gttcaagaga tgtttccgga ccggcaaatc aatttcacca gctacaacat
    34921 tttgacaatc gctgcactgg ttgccaacag tgacatgtta gcgattattc ccagccgttt
    34981 ttataacctg tttagtcgtt gctggccgct ggaaaaactg ccttttccgt ccttaaatga
    35041 ggagcaaata gatttctcta tccactacaa caaattcagc ctgcgtgatc ccatcctgca
    35101 cggagtaata gatgttatcc gtaatgcatt ttgaaaaagt attgttacag ccagatcaca
    35161 gcgcagaacg ataaaatggc acaatccaac aacaatttta cattttcgcg accgctttgg
    35221 ctgctttcag gtccgtttca atgatatact gccagtcgtt aattcaaaaa tagttgataa
    35281 ttacaacaat ctattgaatt gaaacgcttt ccttcgtaat tcgcaactgg aacacgcacg
    35341 ctatgagtaa acccattgtg atggaacgcg gtgttaaata ccgcgatgcc gataagatgg
    35401 cccttatccc ggttaaaaac gtggcaacag agcgcgaagc cctgctgcgc aagccggaat
    35461 ggatgaaaat caagcttcca gcggactcta cacgtatcca gggcatcaaa gccgcaatgc
    35521 gcaaaaatgg cctgcattct gtctgcgagg aagcctcctg ccctaacctg gcggaatgct
    35581 tcaaccacgg cacagcaacg tttatgatcc tcggcgctat ttgtacccgc cgttgtccgt
    35641 tctgtgatgt tgcccacggt cgcccggtag ctcctgatgc caatgaacca gtgaaactgg
    35701 cgcagaccat tgccgatatg gcgctgcgtt atgtggttat cacctccgtt gaccgtgatg
    35761 acctgcgcga tggcggtgcc caacactttg cggattgtat tactgccatt cgggaaaaaa
    35821 gcccgcaaat caaaattgaa actctggtgc cggatttccg cggtcgaatg gatcgtgctc
    35881 tggatattct gactgcaacg cccccagatg tgttcaacca taacctggaa aacgtaccgc
    35941 gtatttaccg gcaggtacgg cctggcgcag attacaactg gtcgctgaag ctgctggaac
    36001 gctttaaaga agcgcatccg gaaatcccga ccaagtctgg tctgatggtg ggtctgggtg
    36061 aaaccaatga agaaattatt gaggtaatgc gcgacctgcg ccgtcatggt gtgacgatgt
    36121 taacgctggg gcaatatttg cagccaagcc gccatcacct gccggttcaa cgttacgtta
    36181 gcccggatga gttcgacgaa atgaaagccg aagcgctggc gatgggcttt acccatgctg
    36241 catgcggtcc gtttgtccgc tcttcttacc acgccgattt gcaggcgaaa gggatggaag
    36301 ttaagtaatc ctgtaataat tacttacatc tgtctgataa aaaacccgct ttttgaagcg
    36361 ggttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc actctttatg
    36421 agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag catcgtcatc
    36481 attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca gcgtacgtaa
    36541 cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt tggtaatact
    36601 aatctcaccc atagatacct tcttgacata taaccggcaa cagaacgcgc cttaaaaacc
    36661 agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac aaggtgaatc
    36721 aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg gttgtttaag
    36781 atgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc cataatgagt
    36841 gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat ccggctttgc
    36901 ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt atccagcgca
    36961 cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccacgaac ccaggcggca
    37021 ggaagtacca ggatttcagc tccctgtaaa gcctgtgcca gcgccagctc tggaaagcgt
    37081 aagtcataac aggtcatcag accgaccttc atcccttcca cctccagtaa cggagctatt
    37141 tcattaccag catcaacatg gcgtgattcc tgaatggcaa atgcatcata gagatgcagt
    37201 ttggcataac gggcgacgat gtttcctgcc tgaagtgcca ccagcatatt ccatgcgcgc
    37261 cccggcgttg aaggaagatg aatcgtcaga attgtcgtca tcatgttacg tttactttct
    37321 cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac cgatagatct
    37381 gcatcatgat catcgcgcgc cagcaatgct tccggcagga caaacagcga tacgtcgttt
    37441 tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca cacagatgta
    37501 acagcaaact gtcctgcagc aaccagcatt gccacctcct gtcagagtcg taaaattcgc
    37561 caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc aggattttgc
    37621 agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg tagcgagatg
    37681 gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga cgctggcagc
    37741 aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca ggatgacgaa
    37801 cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc taacaacctt
    37861 ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg gctgggcatt
    37921 actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg cattctggct
    37981 gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa gcgggttttg
    38041 aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc ctttggcacc
    38101 gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac cattagtctg
    38161 gattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa tgaaaccgaa
    38221 tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca aaattacctt
    38281 tacatgaaaa atagacacaa atgctgtgtc ggttatcagt acaccaattg tggtacgctt
    38341 tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat gttgcttatt
    38401 ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact gaaagcgcat
    38461 ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca tctgaaaagt
    38521 cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga tgtaattcca
    38581 gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat tttcatccgc
    38641 cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt tgttccacga
    38701 gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc aacgcgacag
    38761 gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt tgtcgtaagc
    38821 gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag gctgttgcca
    38881 ggtttgtgca atattttctc taaacgttgt catcctctca tctgcgttag caaaaacttt
    38941 accaacgctg attaactgaa taaaaacaaa ggaaaagata gcgacatatt tactcacgtt
    39001 catttgtgac cataaaacat ttatcaaaaa tctactacta gcatagcaaa gctacaatta
    39061 acataacctt aatagaccca acataaagaa taatctgaat agctgctcta tttatttccg
    39121 cttgttcaag ttaaatattt ccgcgcatcg attaaagatg atgcgtcgtt ttctgcgatg
    39181 ggaatagtca aaaaagaaaa accaagtctt ttttgatgac aaaatgcaat caaggaaaaa
    39241 ttattttatt ttttaacgag atacaacaat catcttaacg aagtatataa tatatatcgt
    39301 caacccatgc tcatattaga ttattttttg agcgcacagc cgcagctaac accattgcaa
    39361 ttaacaaatt tgcatcaatc gccatcaatt tacacacatt attatgtgat aattgccaac
    39421 cgctaaatat gcgttttgtt atctatgtat aaaaacagca acttcaatgt cttaatggca
    39481 gtttttcttg attttaatca gcattcatcg ccaatttatt gggcatattt tttccttaag
    39541 ctttaggaat tttttattat ttactttggg cctggagaca ggaaaaatta tgctgacatt
    39601 cattgaactc cttattgggg ttgtggttat tgtgggtgta gctcgctaca tcattaaagg
    39661 gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta tcagtgccat
    39721 tatggggcac aatgtgttac cgtccagcca ggcttcaaca ggctacagcg ccacggatat
    39781 cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca tgatgattat
    39841 gatgctgtgt ggctttgccg cttacatgac ccatatcggc gcgaatgata tggtggtcaa
    39901 gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga ttgccgccta
    39961 ttttgttgct tgtctgatgt cactggccgt ctcttccgca accggtctgg gtgttttgct
    40021 gatggcaacc ctgtttccgg tgatggtaaa cgttggtatc agtcgtggcg cagcagctgc
    40081 catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg tggtgctggc
    40141 ggcgcaggct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc tgcctatctc
    40201 aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt atctggataa
    40261 aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca ctgcccccgc
    40321 gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg acggtaaatg
    40381 gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg cctccattct
    40441 ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag tggcttatcg
    40501 cgggatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg gggtattcgc
    40561 tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta cctcgtttgg
    40621 ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg cggcagtcac
    40681 gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga aactggcgca
    40741 ctcttccggc attaacccgg cgtatttgac tatcccgatg ctgcaggcgt caaacctggg
    40801 tcgtacccta tcacccgttt ctggcgtagt cgttgcggtt gccgggatgg cgaaaatctc
    40861 accatttgaa gtcgtaaaac gcacctcggt gccggtgctt gttgggctag tgattgttat
    40921 cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca agtaaatagt
    40981 aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta tatgcgttgt
    41041 ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct ggcgcaatat
    41101 tcaagataac gcctggcagt ggtgcggctg atggtcagtg cctgcgccac cgtttctgcc
    41161 gtatgttgca cacaaggctc tttaaacagt tttcgcaccg cgtttagcgt caaggggtca
    41221 atgccggtcg gcagttcgtc tttgggttca ccgcgagcat aggcattaaa catctcatca
    41281 atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg gcggaaacgg
    41341 gttagcgttt gccccagtgt attgattcac ttgaagtacg aaaaaaccgg gagaacattg
    41401 gattattcgg gatctgatgg gattagattt ggtggggctt gcaagcctgt agtacaaatt
    41461 ttaggcgtta accaatgaaa cgcgaaagat agtaaaaaat tgcttttgtt tcattgaaaa
    41521 aacgaaaaac aaaaacacta taaatc
//
LOCUS       DADLSE010000020        40654 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLSE010000020 DADLSE010000000
VERSION     DADLSE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.020"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN692_001069"
     CDS             complement(163..954)
                     /gene="cpoB"
                     /locus_tag="JN692_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7263436.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNATIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN692_001070"
     CDS             complement(964..1485)
                     /gene="pal"
                     /locus_tag="JN692_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7263437.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN692_001071"
     CDS             complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="JN692_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7263438.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGLGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN692_001072"
     CDS             complement(2945..4186)
                     /gene="tolA"
                     /locus_tag="JN692_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7263439.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN692_001073"
     CDS             complement(4251..4679)
                     /gene="tolR"
                     /locus_tag="JN692_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7263440.1"
                     /translation="MARARGRGCRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN692_001074"
     CDS             complement(4683..5375)
                     /gene="tolQ"
                     /locus_tag="JN692_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7263441.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN692_001075"
     CDS             complement(5372..5776)
                     /gene="ybgC"
                     /locus_tag="JN692_001075"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7263442.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN692_001076"
     CDS             complement(5926..6219)
                     /gene="ybgE"
                     /locus_tag="JN692_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7263443.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN692_001077"
     CDS             complement(6219..6332)
                     /gene="cydX"
                     /locus_tag="JN692_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7263444.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN692_001078"
     CDS             complement(6347..7486)
                     /gene="cydB"
                     /locus_tag="JN692_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7263445.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN692_001079"
     CDS             complement(7502..9070)
                     /gene="cydA"
                     /locus_tag="JN692_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7263446.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN692_001080"
     CDS             complement(9919..12552)
                     /gene="mngB"
                     /locus_tag="JN692_001080"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7263447.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKSENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIDSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN692_001081"
                     /pseudo
     CDS             complement(12570..14547)
                     /gene="mngA"
                     /locus_tag="JN692_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            14657..15379
                     /gene="mngR"
                     /locus_tag="JN692_001082"
     CDS             14657..15379
                     /gene="mngR"
                     /locus_tag="JN692_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7263448.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN692_001083"
     CDS             complement(15483..16352)
                     /gene="sucD"
                     /locus_tag="JN692_001083"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7263449.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPEGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN692_001084"
     CDS             complement(16352..17518)
                     /gene="sucC"
                     /locus_tag="JN692_001084"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7263450.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMCDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN692_001085"
     CDS             complement(17612..18829)
                     /gene="odhB"
                     /locus_tag="JN692_001085"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7263451.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN692_001086"
     CDS             complement(18844..21645)
                     /gene="sucA"
                     /locus_tag="JN692_001086"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7263452.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVVTLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSCLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(21946..22662)
                     /locus_tag="JN692_001087"
     CDS             complement(21946..22662)
                     /locus_tag="JN692_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7263453.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN692_001088"
     CDS             complement(22678..24444)
                     /gene="sdhA"
                     /locus_tag="JN692_001088"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7263454.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN692_001089"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24444..24791)
                     /gene="sdhD"
                     /locus_tag="JN692_001089"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7263455.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN692_001090"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24785..25189)
                     /gene="sdhC"
                     /locus_tag="JN692_001090"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7263456.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25570..25677
                     /locus_tag="JN692_001091"
     CDS             25570..25677
                     /locus_tag="JN692_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263457.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25883..27166
                     /gene="gltA"
                     /locus_tag="JN692_001092"
     CDS             25883..27166
                     /gene="gltA"
                     /locus_tag="JN692_001092"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7263458.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27556..28122
                     /locus_tag="JN692_001093"
     CDS             27556..28122
                     /locus_tag="JN692_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7263459.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28173..30524
                     /locus_tag="JN692_001094"
     CDS             28173..30524
                     /locus_tag="JN692_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7263460.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNFNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAEDVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEG
                     HAWLSGVAENQKVTVVWGDSQHCSLHLPEHMEDTANRLILPCH"
     gene            30539..31270
                     /locus_tag="JN692_001095"
     CDS             30539..31270
                     /locus_tag="JN692_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7263461.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVETKK"
     gene            31267..32328
                     /locus_tag="JN692_001096"
     CDS             31267..32328
                     /locus_tag="JN692_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7263462.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            32435..33526
                     /locus_tag="JN692_001097"
     CDS             32435..33526
                     /locus_tag="JN692_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7263463.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN692_001098"
     CDS             complement(33523..34314)
                     /gene="nei"
                     /locus_tag="JN692_001098"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7263464.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN692_001099"
     CDS             complement(34350..35084)
                     /gene="pxpA"
                     /locus_tag="JN692_001099"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7263465.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN692_001100"
     CDS             complement(35074..36006)
                     /gene="pxpC"
                     /locus_tag="JN692_001100"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7263466.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLFHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN692_001101"
     CDS             complement(36000..36656)
                     /gene="pxpB"
                     /locus_tag="JN692_001101"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7263467.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDSARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN692_001102"
     CDS             complement(36679..37422)
                     /gene="ybgI"
                     /locus_tag="JN692_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7263468.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37693..39174
                     /gene="dtpD"
                     /locus_tag="JN692_001103"
     CDS             37693..39174
                     /gene="dtpD"
                     /locus_tag="JN692_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7263469.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPDVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN692_001104"
     CDS             complement(39324..>40654)
                     /gene="phrB"
                     /locus_tag="JN692_001104"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7263470.1"
                     /translation="VLALYIATPRQWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARIQTLAAYEAARKGK"
BASE COUNT         9820 a        11003 c         9853 g         9978 t
ORIGIN      
        1 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
       61 tcagacttga gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg
      121 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
      181 tttttgtgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
      241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
      301 atcagcagcc tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc
      361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
      421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
      481 gcgggattta tcctgcacca gcgcaatagt tgcattgtaa tccgtgtttg catcgccgct
      541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
      601 cgcaccgctt tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
      661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
      721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag
      781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
      841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
      901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
      961 ctcttagtaa accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag
     1021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt
     1081 accctgcagg tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc
     1141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     1201 caggaagttt gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     1261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     1321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     1381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     1441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     1501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     1561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     1621 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     1681 catctaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     1741 tgtgctgctg cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     1801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     1861 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat
     1921 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac
     1981 cagaagccaa atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca
     2041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     2101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     2161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg
     2221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     2281 actgaccgcc gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc
     2341 cggtcagttt ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct
     2401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     2461 gttgataagc aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag
     2521 cgtcaatgcc cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     2581 gtggcagacg agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     2641 cgatgccgcc aatatcttca ggtgccgcac caagccccgc ccactggaaa ggaacaacac
     2701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     2761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     2821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     2881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc
     2941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     3001 gtggtttcgg gatcttagca agtttagctg ctgccaacgc agcctgacaa agtgcgggat
     3061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     3121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     3181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     3241 tcccggcagg cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac
     3301 cagagcttag ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt
     3361 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     3421 ctttcttatc agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg
     3481 cagctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     3541 cagcttctgc cttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg
     3601 cagctgcttc tgccgcttcc gctttcttct tcagtgccgc agcggctacc tcggcttttt
     3661 tctgcgcttc ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgcag
     3721 ccgctttctt cgctgcttct tccgcagctt tagcatctgc cgccgctttc gctgccgcca
     3781 cttcagcttg cttctgcttt aactcggcct gttttgcggc ttcttcagcc tgctttttct
     3841 gctcctgagc cgctaaccgc tctttctcaa gttgcttcag gcgttcctgt tcagccgctt
     3901 gtttctcacg cagttcttca gcagcctgct gttccttcat cttgcgctgt tcatcagaac
     3961 gcttcgcgct tgattcctgg ctttgcatgc gtttgtactg ctcaactacc gcacctgaat
     4021 caaccatgac agcgtcgatg gacgaaccac cgccgcctcc ggctgaagct tctatattct
     4081 catcgaacga actccagatc agcgccgcaa ataagatgac atgcagcact gctgaaataa
     4141 ttatcgcccg cttgagcttg tcgttttgtt cggttgcctt tgacactctc ggtttccaaa
     4201 aactgttcgc ctgttacccg ctctctttca agcaagggaa acgcagatgt ttagataggc
     4261 tgcgtcatta aaccaaccga tttcacaccc gcactatgta acaagttcag tgctttaatt
     4321 atttcatcgt aaggcacatc ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc
     4381 ttgaaacggc tggacacttc cgccaccacc tgctctggtg gtaaacgctc taggcgatct
     4441 ttctcaacca ccacggtgta ctgaccaata ccagacactt caacaatcac tggcggatta
     4501 tcgttactgc tcaccgcctg tgattcagta gcgtctggca gatcgacctc cacgctctgg
     4561 gtgatgatgg gcgctgtcgc cataaagatc agcaacagca ccagcagtac gtccagcaac
     4621 ggtacaatgt tgatttcgga cttgagatcg cgacaacctc gtccacgcgc tctggccatg
     4681 gcttacccct tgttgctctc gctaacggta aacgcctggc ggtgcagaat cgcggtaaac
     4741 tcttccataa agttgtcgta attcagttcc agtttgttta cgcgctggtt gagacggtta
     4801 taagccatta ccgccggaat tgcggcgaac agaccaattg ccgtcgcaat caacgcttct
     4861 gcgataccgg gcgcaaccat ttgcagtgtt gcttgtttta ccgccccgag ggcgataaag
     4921 gcgtgcatga tcccccagac cgtaccaaac agaccaatat acgggctgat ggagccaacc
     4981 gtaccgagga acggaatgtg cgtttccaga ttttcaagtt cacggttcat ggagatacgc
     5041 atagcacgcg acgccccttc cactacggct tccggcgcat ggctattggc acgatgcagg
     5101 cgcacaaact ctttgaaccc gctgtaaaag atttgttccg aaccagtcag attatcccgt
     5161 ttcccctgac tctcttgata gaggcgagag agttcgattc cagaccagaa tttatcttca
     5221 aacgcttcgg cttcacgcgc cgctgcgtta agaatacggg ttcgctggat aataatggcc
     5281 caggatgcga ttgaaaaacc aatcaaaatc aacatgataa gtttaaccag aaggctagcc
     5341 ttcaggaaca aatcaaggat attcatgtca gtcactgctt aaactccgcg acaatagact
     5401 tgggaagcgc acgaggcttc attttgagtg ggtcaacgca aacaaccaga acctctgctt
     5461 cattcagcaa agtattctcg gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac
     5521 gcattgatgt tatttcagtc tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat
     5581 attccaccgt cattttacgt accacaaagg caacgcgttc agccatcagc gcctgctgac
     5641 tgaagtgatg atgacgcagc atctctgtgc gtgctctttc ataaaaagcg acgtaactgg
     5701 cgtggtacac cacaccaccg gcatcggtat cttcatagta gacgcgaacc ggccatcgaa
     5761 acagcgttgt attcacttta catcccggta atgcaacaaa agttagagct tttaaacgct
     5821 gctactatac gcgcgcgatt gaggtttggg aatgagggca agttaaggga gcgtaaaaat
     5881 ttatgggcct ggagcggccc ataatttttt aaagagaatt ctgatttaaa agaagaagaa
     5941 aatcagcccg acaatgagaa caatatcggc aagcaatggg caaaaaatcc cttgccaaag
     6001 aaccttctgc ggacgaaagc ccacgccgtg aatcacacca gcacagacgg cccacatcag
     6061 caataaacca tgccagattt ccagttcact ggtcttcgcg gcaaaacgag atgggtccca
     6121 aaacatacat cctgctaaca gaagcgccat cacgaaggaa agcgcccgca gggggcgctt
     6181 gtccattacc gcatacaagg tcgcgataat cttactcatc agatgtcttc ttgaccggct
     6241 ttgcctgatt cgacgtgttc aagcgccagc gcggtgatta ccccaaacga acaggcaaga
     6301 agcgttccca gaatccatgc gaaataccac atttttagct ccttacttag tacagagagt
     6361 gggtgttacg ttcaatatct tctttggtga tacgaccgaa cattttccag taacaccagg
     6421 cggtgtagag cagaatgatc ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg
     6481 tcagctggct ggaagttgca tcccacattg tcagacttgc gttcatcatg gtgctggacg
     6541 gcatcacaaa cgggaacatt gcgataccgg ctgtcaggat gatgcaggcc agcgtcaggg
     6601 aggagaacac aaacgcccac gcggctttat ccatacgtgc agtcaggatg gtcagcagcg
     6661 gcagaaccac acccagtgcc ggaatagccc acagaattgg cgtgttgttg aagttaacca
     6721 gccatgcgcc agcttcacga accacttctt tattcagtgg gttagaggct gcgtaatggt
     6781 ccattgtcga tttcacgaca taaccatcga taccgtacat cacccatacg ccagccagtg
     6841 cgaaacagac cagtgtcacc agcgcagcca cctgagccgt tgcacgggta cgcaggtgca
     6901 gttcgcccac ggtacgcatt tgcagatagg ttgcgccctg agtaatgatc atccctacgc
     6961 tcaccacgcc tgccagcagg ccgaacgggt taagcaactg gaagaagtta ccggtgtagt
     7021 acagacgcag atattcatca acgttgaacg gtacgccctg caacaggtta ccgaacgcta
     7081 caccaattac cagcggcgga acgaagctac caatgaagat gccccagtcc cacatgttac
     7141 gccagcgggt ttcttcaatc ttggagcggt agtcaaaacc gaccggacgg aagaacaaag
     7201 acgccagcac gaggatcatc gccacataga agccggagaa cgcagcggca tagaccatcg
     7261 gccaggcagc aaagagtgcg ccgcccgcgg tgatcagcca aacctggtta ccgtcccagt
     7321 gtggtgcaat ggagttaatc ataattcgac gctcggtgtc gttacgaccg aggaaacggg
     7381 tgagcatgcc cacccccatg tcgaaaccgt cagtgactgc aaaaccaatc agcagaacgc
     7441 caaccagcag ccaccagata aaacgcaata cttcataatc gatcatttga cgactcctgt
     7501 cttagcgtgc cggctgagta gtcgtggaag actgctcaaa gtgatagcga ccggttttca
     7561 ggctgcttgg gccgaggcgt gcaaacttga acattaagaa caattctgcc accaggaaca
     7621 gggtatacag gccgcaaatc agcaccattg agaagatgag atcgcctgcg gtcagtgacg
     7681 aattcgccac agctgtcggc agcacttcac cgatagccca cggttggcgg ccatattcag
     7741 ccacgaacca gcccgcttct acagcaatcc acggcagcgg aataccgtac agcgcggcgc
     7801 gcagaagcca ttttttctcg ccaatgcggt tgcggatgac actccagaaa gagagcgcga
     7861 tgattgccag cagcaggaag ccacacgcca ccatgatacg gaacgcaaag tacagcggcg
     7921 ctacacgcgg gatggagtct ttggttgcct gttgaatctg cgcttcagtc gcatcagcca
     7981 cgtttggcgt atagcgtttc agcagcagac cgtaaccgag gtctttcttc atgctattga
     8041 actggtcacg aaccgcctgg tcggtagaac cagaacgcag ttgttcgagc agagagtacg
     8101 ccttcatccc gttacgaatg cgttcttcat gctgcaccat cagctctttc aggccgataa
     8161 ccggggtatc cacggaacgc gttgcaatga tgcccagtgc gtaagggatc tgaatcgcaa
     8221 atttgttcgt ctcctcttcc tgatcaggaa tgccgaacag agtaaaggca gcaggcgcag
     8281 gttgcgtttc ccactcggct tcaatagcag ccagtttggt tttctgcacg tcgcccattt
     8341 cgtagccgga ttcatcaccc agaacaataa cagacagaac agcagccata ccgaagctgg
     8401 cagcgatagc aaaggagcgt ttagcgaagg cgaagtcacg acctttcagc atataccatg
     8461 cgctgatacc gaggatgaac atcgcgccag tcacataacc agacgctaca gtgtgaacga
     8521 atttcacctg agcaaccggg ttaagcacca gctcggagaa gctcaccatc tccatacgca
     8581 tagtttcaaa gttgaaatcg gacgcgattg ggttttgcat ccagccgttc gcaaccagaa
     8641 tccacagtgc ggacaggttt gaaccgagcg ccaccagcca ggtgacacac atatgctgaa
     8701 ctttacccag acgatcccaa ccgaagaaga acagacctac aaaggtggat tcgaggaaga
     8761 aggccatcag accttcgatt gccagcggcg caccgaagat atcccctaca tagtgggaat
     8821 agtaagacca gttagtcccg aactggaact ccatggtcag accggtagcc acacccagag
     8881 cgaagttgat accaaacaac ttgccccaga acttggtcat atctttataa atctgtttgc
     8941 cggagaggac gtagaccgtt tccataatgg ccaggaggaa cgccataccg agcgtcagtg
     9001 gcacaaaaag gaagtggtac atcgcggtca aggcaaactg taagcgcgac agttcgacta
     9061 tatctaacat catgactcct tgctcatcgc atgaagactc cgagagtgaa ccccgttaga
     9121 aagggtcaca cgcatgcccc aatagaaatt tgtttgaaca ctttcatcat accatgtgaa
     9181 tgcacacaaa aacggcgtaa agaaaggttg caaacatgtt aataaaaact caaattgatc
     9241 ccacgtatat attacgccgc aaaatcctta caataaacag gtttttgttg aagcaaattt
     9301 gcatttttcg acggtgatca atttatagct aaattgccgc ctttcagcca atttgatcga
     9361 gaacaattta tctctttttg atgcccattt ccaagactta tacattgata aatatcaatt
     9421 tctacaaatt atgttaatca tgtttcaata aggtaacttt agtgacattt atgtttaaaa
     9481 tgtgtgaatt ataaggctga aattagtttc ggttggcaat taaagataaa aagagacgaa
     9541 gcaaaaacag cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag
     9601 tttcaggatt attaataaag gcataaatct cccccccgcc gatattaaca tttaattaac
     9661 ttataattat attataatta actcacccat ggttattatt ttgttaaaaa taaagccaca
     9721 tatacgtggc ttgagagtga agataaacgc aaaattacct gatgcgctac gcttgccagt
     9781 gtctacatca tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt
     9841 catgccgtat ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg
     9901 cttcggtttg attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa
     9961 aaaaggcccc gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat
    10021 cgtttctgag caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga
    10081 ttcagccgga ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc
    10141 gcttatcagg catcccactg gtggcatttt caacaaacta taactttccg gcacgttgaa
    10201 tccggctttg ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt
    10261 cagccatgct cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa
    10321 taaacttagg cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat
    10381 tcccgaaggc cgcctaggcc ttaaaagcag atcttctttc cccagtaagc ccacgccgcg
    10441 cagcaacgta atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc
    10501 ttcgctaaag acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt
    10561 ccataccgga accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc
    10621 gttcacgggg cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa
    10681 agatgtaggg atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc
    10741 cacatcaatg cgcctgttgt tatgactaag agtgacaacc aactcgacac ctactctgcc
    10801 agtggattgt cttgcgctgc gttgttgcag attacgcggc accgccattt catagcggat
    10861 aacagccctg ctctgccagg cctcatgaat aatctcgcat tgcggtttcg cattcgccga
    10921 agtaattacc cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct
    10981 ttcttcaatt tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag
    11041 cgttccatcc tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc
    11101 actttttgcc gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc
    11161 catagaaggg acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc
    11221 gtaatgaact atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa
    11281 atacggtaca ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac
    11341 agtggtgttg atcacttctt cccgcggcca gggcatcagg ttaaacagga cgagtttgtc
    11401 ggcgtcgctc tgcggcatgt tgtcggcgat tttacgcata tagaaacgca gcagattatc
    11461 cgccatgtct tcagccagtt cgaagcgggc gacgatttcg cgatgaactt tgtcactaca
    11521 gcagcagccg atgctgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag
    11581 caagccgtgg tggtattcaa aacccaacgt ccaggccagc gttgccagcg gttccagcag
    11641 attaacaatc ttattttcaa tacgtgcgtg ggcaactttg atatccatgc gcgtggaatc
    11701 gatggtgcga tgcacgcgca tatatttgcc atcaataaat tcccctttca gggttgccag
    11761 attatctcgc tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt
    11821 tcgttgagga taaatttcgc gcagtttctc catcacttcg aagatatttt gctgcaatgg
    11881 catctgatca tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac
    11941 gtcaaaataa ctgtcgaggc gtttgcgtaa tccgttttcg tccgcaggta agtacttacc
    12001 gatggcataa cccaacggca tcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca
    12061 caaaaactcg gttttatccg taccgtggcg ctcagaacat ccacgccaga acatggtgca
    12121 ggtaatgcca aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatcggg
    12181 taaataacct attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag
    12241 attacggacg atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc
    12301 gataatcaac ttgccggctt ctaccagttt cttcacgcgg tctttgtttt ccgatttcac
    12361 cgcgaaataa tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt
    12421 gtcctgttcc agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc
    12481 ttcggtggtg aaataccact cccgatccca gtgcatatgc ggggtgatgt gaacgcgaga
    12541 taccgctttc atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg
    12601 ccatgcttaa ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc
    12661 accaatgccg caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga
    12721 agtaaaaaga gcgagaaaat gccagcaccg ggtgtcgaaa ggccgatatt catcgcaccg
    12781 acaatagcgc ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct
    12841 tcaatcgcca tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat
    12901 ttcccggtct caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt
    12961 acggtaaatg ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg
    13021 ttcgccattg cgcccaagca gaatgcataa gcggctttat tcactggccc tccaaggtca
    13081 aaggaacaca taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg
    13141 ttcagctagg cggtaagcga gttattgatc caggcgacag gttcccccac cacaaacagc
    13201 atcagactgc ccgctcccaa tgtaccgagc accgggtaga gataaaaagt caggaagcca
    13261 ttgaatttac tgctaagacg caagtgattt ttcatccagc gtatcaagta accggctatc
    13321 aatccgccaa cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc
    13381 gcaaagcctg gcgctaacgc ccggtttatc tgccagcgaa taggcggtat aggctgccag
    13441 caccggtacc atcaaaattc cgagcagccc gccgcccagt ttgcggtaca tccacagcca
    13501 ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta ataccgcgac
    13561 cgccagcacc gtcccccccg cgacaatcaa cggtacggca aaagagatcc cgctcaacag
    13621 tgcctgtttc agctccgttt tgacgctttt caccggttgc gtatcttgct gtacggtacg
    13681 cgtctcatcg ccacgctcga gggtaagcga ttgttggatc aacgcttctg catggcgaat
    13741 cggctcggca acagacacag aaagcgcagg aatgccgtta aaacgctcac tctccttgat
    13801 ggcgacttca gccgcaaaaa tacaggcagt tgcactattg agttgatccg ccgttaaacg
    13861 cccttcaata ccgttagcac cttgcttttc aacgtagaca ttcacgccga gtttgcgtcc
    13921 ggctttttcc agatactccg cagccatgta ggtgtgagca atacccgccg ggcaggccgt
    13981 tacgcaaacg atagttggcg cgttggaaaa agaggcagaa ggttgcgtgc ctcccttgtc
    14041 atccagcgcc gagagcaatt catcaggcgt cgtcgccgac tgtatacgcg cccgaatctc
    14101 atcatccgcc aggcgcgtgg tcagcgctgt cagcagttgc atgtgcgtag tacccgcttc
    14161 attgggggga atcgccagca acaccactaa atcaaccgct tccgggccat ccacgccttc
    14221 ccactgaagc ggctcgctga gtgtggcgac cgcaaacgcc gcttctttta ccgcagcagt
    14281 tttgccatgc ggcacagcca acccttcacc taaggcggtc gggccaaggc tttcacggcg
    14341 atacacttct ttcagaaatt gctcagtact ggaaattttc cccagagcag caagacgttg
    14401 agtcaacgcg tgaatggcct cttcacggct ggtaaagcgc gcattcagac acaacgcatc
    14461 gcggtgggtc agagtcgtca gattcatcat gatcctcact tgatcgtttt tatagtcgcg
    14521 ccagtgtgcc cgataaaaca acaccattgc gtgatcaatt tcacttgatt gaaattattt
    14581 acattgtatt tgtattattt acaggatgaa tttatcgggc ataatggcta aattcgtatt
    14641 aatgagatgc taaataatgg gacacaaacc cttataccga cagattgctg atcgcattcg
    14701 tgagcaaatt gcgcgtggcg agcttaaacc tggcgatgcg ttacctaccg aatcggcatt
    14761 acaaacagag tttggcgtca gccgggttac ggtgcgtcag gctttgcgcc agttagtcga
    14821 gcaacagatc ctcgaaagca ttcagggcag cgggacttac gtcaaagaag agcgggtcaa
    14881 ttacgatatt tttcagttaa ccagttttga tgaaaaactg tcggaccgtc acgtcgatac
    14941 acacagtgaa gttctgatat ttgaagtgat tccggctgac gattttcttc agcaacagct
    15001 acaaatcacc gtgcaggatc gtgtgtggca tgtgaagcgc gtgcgctatc gcaagcaaaa
    15061 gccaatggcg ctggaagaaa cctggatgcc gcttgcgttg ttcccggatc tcacctggca
    15121 ggtcatggaa aattcgaaat atcactttat tgaagaagtg aagaagatgg ttatcgatcg
    15181 tagcgaacag gaaatcattc ctctgatgcc aaccgaagag atgagccgtc tgcttaatat
    15241 cagccagaca aagccgattc ttgaaaaagt atcgcgtgga tatttagttg atggtcgggt
    15301 gtttgaatat agccgcaacg cttttaatac cgatgactat aaatttaccc tgatagcgca
    15361 gcgaaaatca tcgcgataag cacaaaaaag gccatcatac gatggccaac catgtcgaaa
    15421 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
    15481 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
    15541 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
    15601 atcgcacccg cgtggcccat acgtttgcct tccggcgcag tcacaccagc gatgtaaccc
    15661 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
    15721 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
    15781 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
    15841 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
    15901 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccgat tttgcattca
    15961 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
    16021 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
    16081 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
    16141 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
    16201 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
    16261 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
    16321 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
    16381 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
    16441 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
    16501 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
    16561 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
    16621 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
    16681 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
    16741 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
    16801 gatcttcctg cgactggtca cacatttcgc gcagatcagg ctggcggaac agggcgttgc
    16861 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
    16921 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
    16981 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
    17041 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
    17101 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
    17161 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
    17221 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
    17281 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
    17341 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
    17401 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
    17461 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
    17521 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
    17581 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggat
    17641 cttccagcaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
    17701 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
    17761 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
    17821 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
    17881 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
    17941 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
    18001 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
    18061 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
    18121 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
    18181 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
    18241 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
    18301 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
    18361 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
    18421 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
    18481 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
    18541 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
    18601 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
    18661 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
    18721 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
    18781 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
    18841 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
    18901 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
    18961 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
    19021 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
    19081 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
    19141 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
    19201 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
    19261 cgccagttct tcgaggcagg aaaccgccag cggatgacgc agcagggatt tcggcgacat
    19321 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
    19381 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
    19441 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
    19501 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
    19561 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
    19621 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
    19681 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    19741 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    19801 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    19861 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    19921 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    19981 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    20041 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    20101 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcaccac
    20161 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    20221 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    20281 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    20341 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    20401 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    20461 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    20521 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    20581 gtggatggtg attggcagca ctttgttgct gctcggctca tcaagtctgt ccagacgagc
    20641 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    20701 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    20761 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    20821 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    20881 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    20941 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    21001 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    21061 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    21121 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    21181 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    21241 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    21301 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    21361 gagctgcagg actttaacct gcttcacatt ggtatcaggg tcggagatcg ttgaagagta
    21421 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    21481 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    21541 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    21601 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    21661 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    21721 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    21781 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    21841 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    21901 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    21961 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    22021 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    22081 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    22141 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    22201 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    22261 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    22321 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    22381 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    22441 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    22501 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    22561 catcatgtca cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    22621 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    22681 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    22741 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    22801 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    22861 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    22921 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    22981 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    23041 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    23101 gttccagcgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgcgcag
    23161 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    23221 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    23281 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    23341 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    23401 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    23461 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    23521 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    23581 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    23641 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    23701 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    23761 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    23821 aatacgccct gccccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    23881 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    23941 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    24001 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    24061 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    24121 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    24181 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    24241 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    24301 agaaacggta tgggaacggg tcgggaagac tttagagagc agcgcacagg tctggccgct
    24361 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    24421 aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg
    24481 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    24541 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    24601 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    24661 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    24721 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    24781 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    24841 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    24901 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    24961 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    25021 ggctggtacc cagaagccac agcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    25081 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    25141 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    25201 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    25261 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ctataactgc gcgtcatgca
    25321 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    25381 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    25441 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    25501 aatttttaat catctaattt gacaatcatt caacaaaatt gttacaaaca ttaccaggaa
    25561 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    25621 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    25681 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    25741 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    25801 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    25861 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaccc tcaacgggga
    25921 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    25981 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    26041 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    26101 gatcgatcag ctggcgaccg attctaacta cctggaagtt tgttacatcc tgctgaatgg
    26161 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    26221 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    26281 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    26341 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    26401 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    26461 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    26521 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    26581 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggt gcgaacccgt ttgcctgtat
    26641 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    26701 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    26761 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    26821 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    26881 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    26941 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    27001 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    27061 cgcccactgg agcgaaatgc acagtgacgg tatgaagatt gcccgtccgc gtcagctgta
    27121 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    27181 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    27241 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaatatatcg
    27301 gtataaatta taaacaaatc gatgattttt aatcatctat cttcctctca gttacatgaa
    27361 taaaccacca aatatagcgc acccctataa tttccaatac tttattcatc aaacatttaa
    27421 ttcataccat caattaatta cttttacatt taataaaatt aataatattt ttaagaaaaa
    27481 ctaaaaacat tcgacaatca aataagatat tcttgcaccg atatactttt ttcttttctg
    27541 ttaaaggact cagttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
    27601 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
    27661 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
    27721 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
    27781 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
    27841 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
    27901 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
    27961 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
    28021 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
    28081 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
    28141 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
    28201 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaatctga
    28261 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
    28321 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaatg
    28381 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
    28441 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
    28501 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
    28561 tgaatattag tattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
    28621 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
    28681 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
    28741 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcatactgat agcgatgata
    28801 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
    28861 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
    28921 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
    28981 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
    29041 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
    29101 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
    29161 aggtttcagc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
    29221 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
    29281 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cagcggcctg ctgctttctg
    29341 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
    29401 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
    29461 taagctaccg ttttaattac agcaaacaag tggatgccac taacagcacg atttccctcg
    29521 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctg gatcacaaat
    29581 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
    29641 ttaccccact aaacttcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
    29701 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
    29761 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
    29821 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
    29881 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
    29941 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc tgacaatgga gcccaggtga
    30001 gcggtaacta tcagcacctg agttcagcgg gtgagtggga tatttctggt acctatgccg
    30061 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
    30121 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
    30181 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
    30241 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggaagacgtt aacgtcatca
    30301 ttcgcaacgc cagcggtcag tttcctcccc tcggagcgga tatccgccag gatgacagcg
    30361 gcattagcgt ggggatggtt ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa
    30421 atcaaaaggt taccgtggtc tggggtgata gccagcattg ctcgctccat cttcctgaac
    30481 atatggaaga caccgcaaat cgcctgattt taccttgtca ttaattaagg aaatgaaaat
    30541 gacatttatg aaaggactac cccttttgtt gttggttgcc agtctgtgca gccacgctgc
    30601 actacaaccc gatcgcactc gtattgtgtt taacgctaat gataaagcta ccagtctgcg
    30661 ggtggataat cgtagcgata aactccccta cctcgcttat tcctggctcg aaaatgaaaa
    30721 aggggagaaa agcgatgatc ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa
    30781 agcaacaacg caggtacgga tagtaaagca agcctcgacc acaaaattac cgggcgatcg
    30841 cgaaacgcta tttttctaca atatgcgtga aattcctccc gccccggaaa aaaacagcga
    30901 ccatgccgtt ctgcaagtcg ccattcagag tcgtatcaag gtgttctggc gaccggctgc
    30961 attacgcaag aaagcaggag aaaaggtcga actgcagtta caggtcagcc agcagggcaa
    31021 ccaactgaca ctgaaaaatc ctaccgcgta ttatctgacc attgcctacc tgggacgaaa
    31081 tgaaaaaggc gttctccccg gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa
    31141 cacaaacacc ggaaattata gcggcagcca gttttatctc ggttatatgg atgattacgg
    31201 cgcgttacgc atgacgacgc tcaactgcag cggacaatgc catttacaag ccgtggagac
    31261 gaagaaatga gtgctggcaa gggagtgttg ctcgtcatct gtctgttatt tctgccgttg
    31321 aagtctgcgc ttgccctgaa ctgctacttt ggttcatccg gtggctcggt agaaaaatca
    31381 gaagcgattc aaccttttgc tgtaccaggc aatgccaaac tcggcgataa gatctgggaa
    31441 tctgacgata ttaaaattcc cgtctattgt gacaacaata ccaacggcaa ctttgaaagc
    31501 gaacatgtct acgcatgggt aaacccctac ccgggggtgc aggatcgtta ttatcaactc
    31561 ggagtaactt acaatggcgt tgattatgac gccaaccagg gaaaaagccg catcgacact
    31621 aaccagtgta tcgacagtaa gaacattgac atttataccc ctgagcagat catcgcgatg
    31681 ggatggcaga acaaaatttg ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt
    31741 ctcgctcgca tgcggttata cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt
    31801 acgcttagcg actatatcgt cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct
    31861 actgcccaaa acctgaaata tcatattact ggtctggaaa acattcgcgt gctggattgc
    31921 agcgtcaatt tttccatttc accggaaaca caggtgattg attttggtaa atttaatttg
    31981 ctggatattc gtcgccacac aatgtcgaaa acattcagta ttaagacgac aaaatcacaa
    32041 aatgaccaat gcaccgacgg atttaaagtc agttcctcct tttataccga agaaacgttg
    32101 gttgaagaag ataaagcatt gttgattggt aatggtctga agctacgttt gctggatgaa
    32161 aacgcctcgc cttatacctt caataaatat gctgaatacg ccgatttcac cagcgacatg
    32221 ttggtctacg aaaaaaccta tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag
    32281 gctgggccct tcgatacagt ggtgcttttt aaaattaact ataactgatt tgccatgatg
    32341 ccctttgcag ccggaaaggg catctctttc agaaaattgc tgattttccc cttttccttg
    32401 attgctgtta ttttttccta gcccggcttt ctttatgctg tatcgataca ccaaagagaa
    32461 aataatgagg gagcgaagga tgccagtttt gcagtgggga atgttatgtg tgctatcact
    32521 tctcctttct attggtttcc tcgcggtaca tctcccggcg gcgctattac ttgggccgat
    32581 gatcgccggg atcatcttca gtatgcgcgg aattactctg caacttcccc gctccgcttt
    32641 tctcgccgcg caggccattc ttggctgcat gattgcgcaa aacctcactg gttcgattct
    32701 caccacgctg gccgccaact ggccgatcgt gcttgctatt ttactggtga cgctgctttc
    32761 cagcgccatc gtgggttggt tattggtgcg ctatagctca ctgcctggaa ataccggtgc
    32821 ctggggttct tctccaggcg gcgcagcggc aatggtcgct atggcgcaag attacggcgc
    32881 agatattcgc ctggtggcgt ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc
    32941 tgttctggtt acccgcatga tgctgggtga taacgctgaa gcggttaacc agcaaatcgt
    33001 ctggttcccg cctgtgagca ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg
    33061 tacggcggga tgcatgctgc gtcttccttc cggcacgatg ctcatcccga tgctggcggg
    33121 cgcagtgctc cagtctggtc agctcatcac catcgaacta ccggaatggc tgctggcgat
    33181 ggcgtatatg gcaattggct ggcggattgg tcttggtttc gataaacaaa tcttactgcg
    33241 ggcactacgc ccgctaccgc aaatcctgct gtcgattttt gctctgctgg ctatttgtgc
    33301 aggtatggcg tggggactga cccggtttat gcatattgat tttatgactg cctacctcgc
    33361 caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc gcagggagca atgccgatat
    33421 ggcgcttatt atggcgatgc aaaccctgcg cctgttcagt attttgctga cggggccagc
    33481 cattgcgcgg tttatttcaa cctatgcgcc gaagcggtcg gcctagtgct ggcagcctgg
    33541 acaccagtaa aacgggcgcg aggacaacgt ggttttctca atgatgccgc cacagcgttc
    33601 gcacagttcg ccatcacgat gaaaaacctt aaagcgaaac agcgccccat gatgcttatt
    33661 ctcatccacc tgcccccgcg tagcgtaaga aaaccgagga atctccagta acgcgtgtgc
    33721 gagtgcatcc agttgcgccg cattgagatc tttcgcttta tgatttccag tcaacccaac
    33781 ctgccagagg atctccaccc gcaaataatt gccaagccca gccagaaacg cctgatcgag
    33841 cagtaatcct gcaaactgac ggttacgaaa gcgcggcgac aataaccgtt cctttaccac
    33901 ctccggcgtc agattcggat ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt
    33961 ggtcagttgt tccggcctca acatctcaat atccgaggcg ctataaagca gaatggtttt
    34021 gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc
    34081 aaccacgcgc cagacgccgt aaagctgatt atggctgtag agcgttaaac cgttgggaaa
    34141 atgagttaac aacgccttac cacgcgtttc cacatgggta acgtgttgac cgataagttg
    34201 tgattgataa gtttttaact gcgggaaggc aaaccagaca tcagttagtg gtttgccttt
    34261 gatcgccgcc tccaggttat ccgctgcacg gcggatctcc gggccttcag gcatggtgat
    34321 atcctttttt cattctttat taagcggggt tatgctgcga caacaatccc ctttttggca
    34381 aatgtagctc gtaatcggcg ggcgaacgcc agagcgtgct cgccgtcgcc atgcaggcag
    34441 accgtttgcg ccgcgaccgt tgcccattcg ccggtgatac ttttgactct gccgtgttgc
    34501 accatctcca gcgtttgcgc caacgcctgt tcttcgtttt caatcaacgc gcctggctga
    34561 cttcgcggca ccagcgagcc gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc
    34621 gttgtcaggc catattgctt gcctgcacga atcagctcgc ttcctgccag cccaacgaga
    34681 accaatgccg gatcgcaagc gtataccgct ctggcgatgg cgtctgccag ttgtgcttct
    34741 ttcgccgcct ggttgtacaa catgccgtgc ggtttgacat gacgcattac gccgccttgc
    34801 gcacgggcaa tcgttgccag cgcgccaatt tgatacagcg tctgggcgta aacggtttcc
    34861 ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg
    34921 ccaatcgcga caccattttt tattgcttca cgcacgcaag cctgcatggt ttgcgcatcg
    34981 cctgcatgaa atccacaagc aatattggca gaggaaacca gcgttaatag ctctgcgtcg
    35041 ctggcgcagc cttcgcccag atcggcgttc aggtcaattt tcattgtgca gccgccacgc
    35101 taattgttca aaataacgtt gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca
    35161 ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc agatggtaca tatcagcctc
    35221 aatgatacag gcaatacgtg gataaccacc ggtggtctgt gcgtcgttca tcaacacaat
    35281 cggctgcccg ttatgcggca cctgcaccac gcccggtaac aaaccgtgaa ataacagttc
    35341 gcgatcggtg atgcgtttta aaatttgccc ctgtaagcga tagcccattc ggttactttg
    35401 cgagctaagc tgccagggcg aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg
    35461 atattccggc cccggcaagg cgcgaatgcg gttgccccac agcagctgtt taacgccctg
    35521 cgcttccata aaatcacgct tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa
    35581 acggccttcc agcccgccaa tccccacttt gagatcggtg ctgcatgacc ccattaccgg
    35641 cggaacatca ataccacccg caaccgccag ataactgcgc atcccgtgct gcgggcgttt
    35701 aagcgttaaa cgctggcctg ctttcatcgg caatcgccag ccggtccaga cggcgttatc
    35761 atccaaccgc gcttcgcaac ctgcacccgt cagggcaaac cacccatcag tttcgaactc
    35821 aactgttaac tgaccgagcg tgatctccag cgcgggggca ttggcgtcat tacccaccag
    35881 caggttagca atgcgcagcg caggcatatc cagtgcgcca cagtggctga tacccgactg
    35941 gcgaaaaccg tgacgaccgc catcctgcac agtggtgtac atgcccgcac gaataatctt
    36001 cagcatattc cctccttctg cggtacaaat cgcacgctgt ctcccggacg taataagatg
    36061 ggttcgtcac gcgccgaatc aaacaggctg agtgaggtat gaccaatcaa ctgccagcca
    36121 cccggcgttg ccagcggata aacaccagtc tgcggcccgc cgatcccgac agaacctgcc
    36181 ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg
    36241 agatacggga agcccggttg aaaacctaaa aaccagacca cgtattccac ggaggagtgc
    36301 aattcaacaa cctgtttttc gctcaacccg caatacgccg cgaccaccgc caaatccggt
    36361 ccgcctgcac caccgtaaac caccggaatt tcaataaagc gagactcagg ttccagggcc
    36421 tcgctctcct cccaccagcg ttgcaaacgc tctatggcat ccagcgccag cgactcagga
    36481 ttacgcaaaa tcaccgtgat attgttcatg ccgggaatgg cttcaaccac attcggcata
    36541 tccaccagac gctgcgccag tcgccagatc cgtttctggc tagccagcgt caccggcggt
    36601 tccagttcca gcactaccgc cgtttcacct atcagataac aacgcgctcg ttgcactttc
    36661 gatccctctg attattcgtt atgcaggatt aggaatatca ataaaggtca catcaagatc
    36721 ggtattttca ttcagccact cgctcaatgc gcgaatacca ccgcgttcag tggcatggtg
    36781 acctgcagca taaaaatgca atccctgctc gcgggctgaa tgaatggtct gttcagaaac
    36841 ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg ctatcgataa aactttgccc
    36901 gccgcccgtg caccaggcga cgcgctggac cacctcaggt ccggtatcgc cacaccataa
    36961 cggcttacgt cccagacgcg cttcaatcca ggaggctagt tccattcccg gcacaggcat
    37021 ggtcagctct ccccacggca ccaacggctc aatttcgccc atgaccgtga tccccagtaa
    37081 cgccgccagc tgtgcgttat tgcccagctc aggatgtgcg tcaagcggca aatgccagcc
    37141 atacaggttg atatcattcg ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat
    37201 gaccggagac tctcctttcc agaagtagcc atgatgcaca atgaccgcat ccgcgcccag
    37261 acgcactgcc tcatcgagca aagcctggct ggcggtgaca ccggtaacaa ttttttgcac
    37321 cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt
    37381 caatttttcg ttaatcagtt gttccagttc ggtgtttttc atcttcgcgc cttccgtatt
    37441 ctgatagcta caccgttaac atagcgttac ctcgcctgtt cgtcgatagc caatcttttg
    37501 acatattaag aaacaaaccc gacgccaaaa gacgcaaatt gtttatatca tcacgcttca
    37561 gctaatgtat atttatgcaa tcagacagca tattgcataa ataatcagat ctgtatcaaa
    37621 gttttaactt taaggaaaca caagcacgcc gcgcaactat tacgtgacgt tgccgtcaat
    37681 tgtggaatta ttatgaataa acacgcatca caaccgcgcg ctatttacta tgtcgttgcg
    37741 ctgcaaatct gggaatattt tagcttttac ggcatgcgtg ccctgctgat tctctatctc
    37801 accaatcaac taaaatacaa cgatactcac gcctacgagt tatttagcgc ctactgttcg
    37861 ctggtgtatg tcacgccaat cctcggtggc tttttggcgg ataaagttct cggcaatcgc
    37921 atggcggtga tgctgggggc gttgttgatg gcgatcggtc atgtggtgct gggtgccagt
    37981 gagatccatc cgtcattcct ctatctgtcc ctggcgatta tcgtctgcgg ctatggcctg
    38041 tttaaatcta acgtaagctg tctgctcggc gagctgtatg agccaaccga tccgcgtcgt
    38101 gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg ggtctattat cgcacctatc
    38161 gcctgtggtt acgcccagga agagtacagc tgggcgatgg gctttggcct ggcggcggtt
    38221 ggcatgatcg cgggtctggt cattttctta tgtggcaatc gtcatttcac tcatacccgc
    38281 ggcgttaaca aaaaagtact gcgtgcgaca aactttctcc tgccgaactg gggatggctg
    38341 ctggttctgc tggtggcaac gcctgcactg attaccgtgc tgttctggaa agagtggtcg
    38401 gtatacgcct taattgtcgc gactattatt ggcctgggtg tactggcaaa aatttatcgc
    38461 aaagcagaaa accagaaaca gcggaaggag ctggggctga ttgtgacgct caccttcttc
    38521 agtatgttgt tctgggcctt cgcacaacag ggcggtagct cgattagcct ttatatcgac
    38581 cgcttcgtta accgcgatat gtttggttat accgttccga ccgcgatgtt ccagtcgatt
    38641 aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc
    38701 gcaggtaatc gtaccgtgcg catctgggga aaatttgctc ttggccttgg cctgatgagc
    38761 gccggattct gcattctgac cttaagcgcc cgctggtccg caatgtatgg tcactcttct
    38821 ctgccactga tggtgttagg cctcgcggtg atgggctttg cggaactgtt tatcgacccg
    38881 gttgccatgt cgcaaattac gcgcattgaa attccggacg tgaccggcgt attaaccggc
    38941 atctacatgc tgctttctgg cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag
    39001 acatcgcagg cttcgtttga tgcttccggg gcgatcaact actccatcaa tgcatatatt
    39061 gaagtatttg atcaaattac ctggggcgca ctggcgtgtg taggactggt actgatgatt
    39121 tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg cgctggagtc ataataggcc
    39181 agcaccgatg cctgatgcga cgcttgacgc gtcttatcag gcctacgtat ttcctgcaat
    39241 ttattgaatt tgcacaaatt tgtaggctgg attaggcgtt cacgccgcat ccggcatctg
    39301 ggctcgattg cctgacgcgt ctgttatttc cccttccgcg ccgcctcata cgctgccaac
    39361 gtttgtattc tcgcctcttt gtgctcgact atcggttgcg gataatccag cttcacacct
    39421 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    39481 ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt tctcgccctg agttatcgga
    39541 ttgaaaatac gaaaatacgg cgcagcatca gtacccgtag aagccgccca ctgccagcca
    39601 ccgttattag ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    39661 cgccagtcga tcaacaaatc tttcaccaga aaactggctg taatcatccg tagccggtta
    39721 tgcatccagc cagtgctgtt aagctgacgc atagcggcat caataatcgg gtatcccgtt
    39781 ttgcctttct gccaggcctg taaatgtgcg ggattgcttt gccactgcac acgatccgtc
    39841 caggcaataa atggacgatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    39901 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    39961 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccaag
    40021 ctggcggaca aacggctggt gccttccact gccggaaaat ctcgttgttg ctcatactct
    40081 ccagcaccgt tctcgcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    40141 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgcgggcttt
    40201 atcgatccgc tactacgaac ttttggtgca gcgacgcact ccggcatccc ttcccgcagc
    40261 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attgccggtc
    40321 atcaccgccc ccggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    40381 agcgctcttt caacgtccac atcccgcgct cgctcattca cctcatactg atagttataa
    40441 aaaagatgag taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    40501 aagtcatcca cttcacggaa caataaagga ataccttttt ccgccagcgc gatttgtagc
    40561 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtctcccac
    40621 tggcgtggtg tagcgatata cagcgccagc acgc
//
LOCUS       DADLSE010000021        40185 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLSE010000021 DADLSE010000000
VERSION     DADLSE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.021"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..524)
                     /locus_tag="JN692_001105"
                     /pseudo
     CDS             complement(1..524)
                     /locus_tag="JN692_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
     gene            complement(983..1069)
                     /locus_tag="JN692_001106"
     tRNA            complement(983..1069)
                     /locus_tag="JN692_001106"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1033..1035),aa:Leu,seq:gag)
     gene            complement(1084..1416)
                     /gene="secG"
                     /locus_tag="JN692_001107"
     CDS             complement(1084..1416)
                     /gene="secG"
                     /locus_tag="JN692_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7263471.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLTTLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1644..2981)
                     /gene="glmM"
                     /locus_tag="JN692_001108"
     CDS             complement(1644..2981)
                     /gene="glmM"
                     /locus_tag="JN692_001108"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7263472.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2974..3822)
                     /gene="folP"
                     /locus_tag="JN692_001109"
     CDS             complement(2974..3822)
                     /gene="folP"
                     /locus_tag="JN692_001109"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7263473.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3912..5846)
                     /gene="ftsH"
                     /locus_tag="JN692_001110"
     CDS             complement(3912..5846)
                     /gene="ftsH"
                     /locus_tag="JN692_001110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7263474.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMPEQLGDK"
     gene            complement(5946..6575)
                     /gene="rlmE"
                     /locus_tag="JN692_001111"
     CDS             complement(5946..6575)
                     /gene="rlmE"
                     /locus_tag="JN692_001111"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7263475.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6701..6994
                     /gene="yhbY"
                     /locus_tag="JN692_001112"
     CDS             6701..6994
                     /gene="yhbY"
                     /locus_tag="JN692_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7263476.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7150..7626)
                     /gene="greA"
                     /locus_tag="JN692_001113"
     CDS             complement(7150..7626)
                     /gene="greA"
                     /locus_tag="JN692_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7263477.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7874..9307
                     /gene="dacB"
                     /locus_tag="JN692_001114"
     CDS             7874..9307
                     /gene="dacB"
                     /locus_tag="JN692_001114"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7263478.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9493..10653)
                     /gene="cgtA"
                     /locus_tag="JN692_001115"
     CDS             complement(9493..10653)
                     /gene="cgtA"
                     /locus_tag="JN692_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7263479.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDGEDWDEDDEEGVEFIYKR"
     gene            complement(10669..11634)
                     /locus_tag="JN692_001116"
     CDS             complement(10669..11634)
                     /locus_tag="JN692_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7263480.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11761..12018)
                     /gene="rpmA"
                     /locus_tag="JN692_001117"
     CDS             complement(11761..12018)
                     /gene="rpmA"
                     /locus_tag="JN692_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7263481.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12039..12350)
                     /gene="rplU"
                     /locus_tag="JN692_001118"
     CDS             complement(12039..12350)
                     /gene="rplU"
                     /locus_tag="JN692_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7263482.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12609..13580
                     /gene="ispB"
                     /locus_tag="JN692_001119"
     CDS             12609..13580
                     /gene="ispB"
                     /locus_tag="JN692_001119"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7263483.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13809..14087
                     /gene="sfsB"
                     /locus_tag="JN692_001120"
     CDS             13809..14087
                     /gene="sfsB"
                     /locus_tag="JN692_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7263484.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14135..15394)
                     /gene="murA"
                     /locus_tag="JN692_001121"
     CDS             complement(14135..15394)
                     /gene="murA"
                     /locus_tag="JN692_001121"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7263485.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15449..15703)
                     /gene="ibaG"
                     /locus_tag="JN692_001122"
     CDS             complement(15449..15703)
                     /gene="ibaG"
                     /locus_tag="JN692_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7263486.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15863..16156)
                     /gene="mlaB"
                     /locus_tag="JN692_001123"
     CDS             complement(15863..16156)
                     /gene="mlaB"
                     /locus_tag="JN692_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7263487.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16156..16791)
                     /gene="mlaC"
                     /locus_tag="JN692_001124"
     CDS             complement(16156..16791)
                     /gene="mlaC"
                     /locus_tag="JN692_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7263488.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QVYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16810..17361)
                     /gene="mlaD"
                     /locus_tag="JN692_001125"
     CDS             complement(16810..17361)
                     /gene="mlaD"
                     /locus_tag="JN692_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7263489.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17366..18148)
                     /gene="mlaE"
                     /locus_tag="JN692_001126"
     CDS             complement(17366..18148)
                     /gene="mlaE"
                     /locus_tag="JN692_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7263490.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18156..18965)
                     /gene="mlaF"
                     /locus_tag="JN692_001127"
     CDS             complement(18156..18965)
                     /gene="mlaF"
                     /locus_tag="JN692_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7263491.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19175..20152
                     /locus_tag="JN692_001128"
     CDS             19175..20152
                     /locus_tag="JN692_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7263492.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20166..21152
                     /gene="kdsD"
                     /locus_tag="JN692_001129"
     CDS             20166..21152
                     /gene="kdsD"
                     /locus_tag="JN692_001129"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7263493.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRNALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21173..21739
                     /gene="kdsC"
                     /locus_tag="JN692_001130"
     CDS             21173..21739
                     /gene="kdsC"
                     /locus_tag="JN692_001130"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7263494.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21736..22311
                     /gene="lptC"
                     /locus_tag="JN692_001131"
     CDS             21736..22311
                     /gene="lptC"
                     /locus_tag="JN692_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7263495.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22280..22837
                     /gene="lptA"
                     /locus_tag="JN692_001132"
     CDS             22280..22837
                     /gene="lptA"
                     /locus_tag="JN692_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7263496.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22844..23569
                     /gene="lptB"
                     /locus_tag="JN692_001133"
     CDS             22844..23569
                     /gene="lptB"
                     /locus_tag="JN692_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7263497.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23617..25050
                     /gene="rpoN"
                     /locus_tag="JN692_001134"
     CDS             23617..25050
                     /gene="rpoN"
                     /locus_tag="JN692_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7263498.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25073..25360
                     /gene="hpf"
                     /locus_tag="JN692_001135"
     CDS             25073..25360
                     /gene="hpf"
                     /locus_tag="JN692_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7263499.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25478..25969
                     /gene="ptsN"
                     /locus_tag="JN692_001136"
     CDS             25478..25969
                     /gene="ptsN"
                     /locus_tag="JN692_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7263500.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTESTP
                     DEA"
     gene            26015..26869
                     /gene="rapZ"
                     /locus_tag="JN692_001137"
     CDS             26015..26869
                     /gene="rapZ"
                     /locus_tag="JN692_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7263501.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26866..27138
                     /gene="npr"
                     /locus_tag="JN692_001138"
     CDS             26866..27138
                     /gene="npr"
                     /locus_tag="JN692_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7263502.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27351..27983
                     /gene="yrbL"
                     /locus_tag="JN692_001139"
     CDS             27351..27983
                     /gene="yrbL"
                     /locus_tag="JN692_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7263503.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27980..28708)
                     /gene="mtgA"
                     /locus_tag="JN692_001140"
     CDS             complement(27980..28708)
                     /gene="mtgA"
                     /locus_tag="JN692_001140"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7263504.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGSEPFMQQHQLD"
     gene            complement(28705..29358)
                     /gene="elbB"
                     /locus_tag="JN692_001141"
     CDS             complement(28705..29358)
                     /gene="elbB"
                     /locus_tag="JN692_001141"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7263505.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKELAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29588..31924)
                     /gene="arcB"
                     /locus_tag="JN692_001142"
     CDS             complement(29588..31924)
                     /gene="arcB"
                     /locus_tag="JN692_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7263506.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32020..32949)
                     /locus_tag="JN692_001143"
     CDS             complement(32020..32949)
                     /locus_tag="JN692_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7263507.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGIRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33624..38084
                     /gene="gltB"
                     /locus_tag="JN692_001144"
     CDS             33624..38084
                     /gene="gltB"
                     /locus_tag="JN692_001144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7263508.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDMTFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38097..39515
                     /locus_tag="JN692_001145"
     CDS             38097..39515
                     /locus_tag="JN692_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7263509.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39699..40178
                     /locus_tag="JN692_001146"
     CDS             39699..40178
                     /locus_tag="JN692_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7263510.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT         9962 a        10847 c         9922 g         9454 t
ORIGIN      
        1 atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata
       61 aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa
      121 catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat
      181 aagggatata catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa
      241 tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca
      301 tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg
      361 atatacgttt aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg
      421 caccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc
      481 aatgagattt aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc
      541 acaaccaata taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact
      601 ctgatccata tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc
      661 gttctcagta attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca
      721 tcagtagtta tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg
      781 taccggaact ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta
      841 taactaaggc gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac
      901 gagatacaga tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt
      961 tcagcgtctc ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt
     1021 gggcactacc acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac
     1081 tttttagttc gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt
     1141 tttcgccggt gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat
     1201 gttacccagc accagactga tgatgaagaa taacgttgtc agcagcgccg tcatgcgggt
     1261 catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga
     1321 ggctcccata tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc
     1381 cacaataagg aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt
     1441 gccgcgtacc aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg
     1501 gtgtgaatac taaccaaagc gaatgacctt cgcaagggca attttatcgc attgtatcaa
     1561 ctgcggaaaa aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc
     1621 tttttagcca gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca
     1681 aattcagtca cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg
     1741 ccggatttac gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc
     1801 acggctttaa ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta
     1861 accagaatct gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta
     1921 cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta
     1981 tccagcagga tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt
     2041 ttttccagta cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc
     2101 agcgccagtt caagccccat gttgctcatc aatgtaccca cagcgccacc acgcagctgg
     2161 ccctgacgaa gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg
     2221 ccttcatggt caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc
     2281 gctttttcag ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca
     2341 ttgatgttta caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc
     2401 agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt
     2461 tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg
     2521 gcatcaacga tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt
     2581 tccatttccg cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag
     2641 aagaatttaa tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc
     2701 tctgcgcgga aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag
     2761 agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca
     2821 gaaatacgcg tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta
     2881 cccgcggccc aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga
     2941 ccacgaatcc catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc
     3001 agacagagtg gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg
     3061 aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg
     3121 ctcggacggc cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa
     3181 cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga
     3241 gagattttta ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc
     3301 ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc
     3361 atacttcgga gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg
     3421 taaaccggtt tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt
     3481 aataatgtga gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac
     3541 cgagatccag acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa
     3601 ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat
     3661 gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt
     3721 atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg
     3781 atggctaagg tccagtgaag taccctgggc aaagagtttc atgatgttat ccctggtatg
     3841 aattgataag aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct
     3901 gatgcgggaa cttacttgtc gcctaactgc tctggcatgg tgttacccgg gttcggcgta
     3961 cgcggttcat caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa
     4021 gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca
     4081 atctgcggtg cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata
     4141 tccatattgt cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc
     4201 acttcctggt cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta
     4261 cggccgagga atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag
     4321 aagccccact gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac
     4381 gcaccggtag aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac
     4441 agcgtagaaa tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc
     4501 aagaagaaag tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga
     4561 tcgtgttccg gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat
     4621 tctttctgcg cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct
     4681 ttcgctttct cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt
     4741 gccgcttcgt tcaccaggtt cgctaggtca gcaccggaga aaccaggagt accacgggca
     4801 atgattgccg cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc
     4861 tgctcacgac cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga
     4921 cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct
     4981 tcgttacctt cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca
     5041 tcgtgaccac cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata
     5101 aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca
     5161 cccacaccga cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc
     5221 gcttcgcctg caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc
     5281 aggacgcctt tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac
     5341 tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc
     5401 gttttgatct gatcttctgt cagcatgcgc gctttgctct taccaaacga catggcacct
     5461 ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc
     5521 atcgggaacc aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg
     5581 acaaccttga cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg
     5641 taagtggtat aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc
     5701 gcttcacgaa cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg
     5761 ccattagact cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc
     5821 agccagagta ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt
     5881 gttaaaaaca gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct
     5941 cccggttagg gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa
     6001 gagtccggct tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca
     6061 tcgaaacctt cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca
     6121 cgacacattt ctagcgccag ttccaccaga tacatggcac gggggatatc caccgccggt
     6181 gttccgctca tgtttggtgc catatcagac atgacaacct ggactttgct gtcgccaacg
     6241 cgctccagca gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca
     6301 ccaacgatag gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt
     6361 tgggtgacca catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt
     6421 cccggtttaa agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa
     6481 cgtaatccct ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag
     6541 cggctggagc tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca
     6601 tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta
     6661 gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca
     6721 aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa
     6781 tggtttgacc gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat
     6841 caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt
     6901 gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc
     6961 aactaaagaa cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg
     7021 ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg
     7081 ataaaaggcc gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt
     7141 gggtaattct tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt
     7201 tttgatgacc acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt
     7261 tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg
     7321 ttcttcgtca gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt
     7381 gttgggcatt ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt
     7441 gatacggcct tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt
     7501 caggtcgcca tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga
     7561 tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc
     7621 ttgcatttgt tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg
     7681 ttcggcctgc ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt
     7741 acaagtccca ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga
     7801 agggtcatcg tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg
     7861 ttagcgcgag attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt
     7921 cagtgttcag gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct
     7981 tgccctgatg gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca
     8041 gatggcgctg cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact
     8101 cggccccgat tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt
     8161 acttaagggt gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat
     8221 tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt
     8281 gatagatacc tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat
     8341 gacacaatgc tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt
     8401 ctcgctctac agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta
     8461 ccccgttacg atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata
     8521 ctgcgaactg gatgtggtgc caggcgacct gaaccgcttt acgctgacgg gatgcctgcc
     8581 acaacgttct gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg
     8641 tgcaattctg aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg
     8701 ccagactcag gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca
     8761 cgatctgctt aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg
     8821 catgatcggc catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt
     8881 gcgtcagatc ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg
     8941 ttcagggctt tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata
     9001 cattgcccaa cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga
     9061 cggctctttg cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc
     9121 gaaaaccggt tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg
     9181 gcaacgaatg gcatttgtac aatatctttc tggctatgca gtagaacccg cggatcagcg
     9241 taatcgccgt attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa
     9301 caattagtca aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg
     9361 ctttgtttat gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc
     9421 aatatattgc aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt
     9481 ggtcattagt gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca
     9541 gtcttcacca tcctcttcag caatctcttc aagctgctga cggtgataat catcccacat
     9601 gaattcgact ttctctggct gtttcgcttc ttcagcctgc acgaccgggt tttcaatgat
     9661 aaaggtcatc acatcccagc agagatcttt cacgcccagt ccgctcgccg cagagatcag
     9721 ataatattta tcttcccagc ccagcgcttc agcgatcgct ttcgctttct cttcggcttc
     9781 tgccttatcc agcagatcga tcttgttgaa gactaaccaa cgcggtttcg ccgccagatc
     9841 ctggctgtat ttttccagct cgctgataat aatacgcgcg ttttcaaccg gatcggtgcc
     9901 gtcaatcgga tcgatatcga tgaggtgcaa caggacgcgg caacgttcca ggtgcttcag
     9961 gaagcgaatg cccagacctg cgccttccgc agcgccttca atcagtcctg gaatatcggc
    10021 aacaacgaag ctcttttcgt tgtccattcg taccacaccc agacttggca ccagagtggt
    10081 aaacggataa tccgccactt tcggtttagc cgccgatacc gcacgaataa aggtcgattt
    10141 acccgcgttt ggcatcccca acataccgac gtcagccagc agcatcagct ccagcagcag
    10201 ctcacgctta tcgcccggcg taccgttagt tttctgccgt ggtgtacggt taactgacga
    10261 tttgaaacgg gtattgccca gaccgtgcca gccgccctta gcaaccagca gacgctgacc
    10321 gtgtttagtc atatcgccca tggtttcacc agtaccctgg tcgattacac gcgtacctac
    10381 cggcacttta atcgtcacgt ctttaccgcg cttaccggta caatcgcggc ttgcgccgtt
    10441 ctgaccgcgc tctgcacgga aagatttttc aaaacgataa tcgataagcg tgttcaggtt
    10501 ctcgtcggct tccatccata cgtcaccacc atcaccgccg tcgccgccat ccgggccgcc
    10561 tttcggaata tacttttcgc ggcggaagct cacgcaacca ttaccgccat cacctgcaac
    10621 gaccagaatc gatgcttcat caacaaactt cattttattc tccgtaaatc attcgcctgc
    10681 gcggggttgc gataccaccg ttgtatgctt acgtaatccg ccccaaatac gatgaccaat
    10741 ggcggaatac atcgcgcccg caaccacgac aaacgcaccg agataaccta aaaggtttaa
    10801 catcggtctg gcgaagaaat cgggccaggc cagtgataaa agatctgaaa aaaacagcgt
    10861 aaacagtggg gtgagcgtga tgatcgcgct cacctgcgct gcctgccagc gagccatcgc
    10921 ttccgccagg gcgccatatc ctaccaaggt attcagtccg caaaaaatta aacatgcgag
    10981 ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc agagggaaga gcgcgattgt
    11041 acataaagtg tacagtaaaa acaggatctg cggtgaggcc agccgacgca ataaaacctt
    11101 ttgcgccacg ccataactca cccaaaccgt cgccgcaccg accccaaaga taactcccca
    11161 ggtgtaatcg gtgagctttg taaatatctc gaccagactg gtgttaaaaa acatcaccag
    11221 accgctcagg agcatcaatg cccctacaac ctgagtgctg cgcattttct ctttcaggat
    11281 aaatacgctg gcaaccatca tgccaactgg cgagagttgc ccaatcacct gcgaagcggt
    11341 cggactcagg tattgcaagg atgagctgaa caggatgaag ttcccaaaca gcccggcggt
    11401 cgccactgcc aacaaaatca accagcgtgg cttacgaaac acgcgtaatg gcggcaacct
    11461 cttcttcacc gcaagaatgg caccaaggcc aatactcgcc atcaagaaac ggtaaaacac
    11521 gattgtcgga ggttccatca cctccagcac ctgcttcatt gcgattggca acgcccccca
    11581 gcaaattgct gtggtaagcg ccaaaagaat gccaatgcct gcctgctgct tcataccgta
    11641 ttccctgcaa agcgcatttg cggattaacg tcgcaccatg cgcattttta ccggttatcg
    11701 aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc gttaccggga cgcgaaaaac
    11761 ttattcagct tcgatgctga taaatttacg gtttttcggg cctttaactt cgaatttcac
    11821 tttaccgtct gctttagcaa acagagtgtg gtcacgaccg caacctacgt tagcgccagc
    11881 gtggaatttg gtaccacgtt gacgaacgat gatgctaccc gccagaacgg attcgccacc
    11941 gaaacgctta acgcccaggc gtttagcttc tgaatcgcga ccgttacgtg tggagccgcc
    12001 agccttttta tgtgccattt gaaatctctc ctcaggtctt aggcgctgat gccagtaatt
    12061 ttcacatcag tgaaccactg acgatggccc tgctgcttac gatagtgttt acgacgacga
    12121 aacttaacga ttttaacttt ctcgccacga ccgtgagcaa caacttcagc tttgattacg
    12181 ccgccatcaa cgaaaggaac gccgattttg acttcttcac cgtttgcgat catcagcact
    12241 tcagcgaact caacagtttc gccagttgcg atgtccagct tttccaggcg aacggtctga
    12301 ccttcgctta ctcggtgttg tttaccacca ctttggaaaa ccgcgtacat ataaaactcc
    12361 gcttctgcgc acaccttttc aatgattcag agtgcgctat aaatattcac aatagggcgc
    12421 gaatattacg caaaacgcac gcctttgaca agtgctacag tcaatacacg aagaaaaaaa
    12481 acacaacttg tacggtaacg tttatctgtg ccattttttc agtacaatta ccctatattc
    12541 ctaaccataa accctaagtt gcctttgttc acagtaaggt aatcggggcg aaaagcccgg
    12601 cttttgcgat gaatttagaa aaaatcaatg agttaaccgc gcaagatatg gcgggtgtta
    12661 atgcggcaat ccttgagcag cttaattccg acgtccaact gatcaatcag ttaggctatt
    12721 acatcgtcag cggcggcggt aaacgtattc gtccgatgat tgctgtactg gctgcacgag
    12781 ctgttggcta tgagggaaat gcgcatgtca ccattgctgc cctgatcgag tttatccaca
    12841 cggcgactct gctacacgac gacgttgtgg atgaatcaga tatgcgcagg ggtaaagcta
    12901 ccgccaacgc cgcatttggc aatgccgcca gcgtgctggt aggcgatttt atttataccc
    12961 gcgcgttcca gatgatgacc agcctcggtt cactcaaagt gctggaagtc atgtcagaag
    13021 ctgtaaacgt catcgcagaa ggtgaagttc tgcaactgat gaacgttaac gatccggaca
    13081 tcactgaaga aaactacatg cgcgttatct atagcaaaac cgcgcgtctg tttgaggctg
    13141 ccgcgcagtg ttccgggatt ctggctggct gtacgccgga ggaggagaaa ggcctgcagg
    13201 attatgggcg ctatctcggc actgctttcc agttgatcga cgatttactc gattacaatg
    13261 ccgatggcga acagttaggt aaaaacgtcg gcgacgatct gaacgaaggt aaaccgacgc
    13321 tgccgctgct gcatgcgatg catcatggca caccagaaca ggcacagatg atccgtaccg
    13381 ccatcgaaca gggtaacggt cgccatcttc tggaaccggt tctggaagca atgaacgctt
    13441 gtggatctct tgaatggacg cgtcagcgcg ccgaggaaga agcagacaaa gccatcgcag
    13501 cgttacaggt gctcccggac accccttggc gagaagcact catcggcctc gcgcacatcg
    13561 ctgttcaacg cgatcgttaa tcccctccct tcatcccgcg cagtgcgcgg gatgattcca
    13621 gtaaattcca taaaaacact aattcagcgc tataacacct gaaaaaaaag ggcgatgaat
    13681 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa taggccatca
    13741 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    13801 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    13861 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    13921 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    13981 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    14041 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    14101 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    14161 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    14221 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    14281 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    14341 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    14401 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    14461 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    14521 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    14581 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    14641 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    14701 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    14761 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    14821 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    14881 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    14941 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    15001 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    15061 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    15121 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    15181 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    15241 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    15301 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    15361 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    15421 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    15481 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    15541 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    15601 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    15661 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    15721 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    15781 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    15841 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    15901 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    15961 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    16021 ggctaagatc gatgcaggta atccccttca ccgcttcctc acgcatttcc caaagcggta
    16081 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    16141 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    16201 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    16261 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    16321 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    16381 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    16441 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa acctgcttca ggtactcacg
    16501 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    16561 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    16621 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    16681 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    16741 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    16801 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    16861 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    16921 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    16981 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    17041 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    17101 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    17161 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    17221 atcgaacgtc gcataaagcg tgtaggtcgg ttcagtacgt atggacgtca cgttcgccgc
    17281 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    17341 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    17401 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    17461 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    17521 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    17581 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    17641 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    17701 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    17761 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    17821 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    17881 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    17941 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    18001 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    18061 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    18121 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    18181 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    18241 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    18301 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    18361 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    18421 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    18481 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    18541 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    18601 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    18661 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    18721 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    18781 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    18841 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    18901 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    18961 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    19021 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    19081 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    19141 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    19201 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    19261 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    19321 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    19381 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    19441 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    19501 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    19561 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    19621 ggctgaacgt caggggactg acagcctgac cagagagcag cttgcagagc tgccgcgtga
    19681 cggcggattg cccgtcgcgt ttttatggct cggcattgcg cttatcatca tgccagtggc
    19741 cacgcggatg gtggttgata acgccacggt gctggcgaat tactttgcca tcagcgagtt
    19801 gacgatgggt ctgacggcaa ttgctatcgg aaccagtctg ccggaactgg caaccgcaat
    19861 agcgggggtt cgcaaaggtg aaaacgacat tgctgtcgga aatatcattg gcgcaaacat
    19921 ttttaatatt gtcatcgtgt tgggtttacc cgcgctgata acgccaggag agattgatcc
    19981 actggcgtac agtcgtgact acagcgtgat gttgctggtg agcattattt ttgcgttgct
    20041 gtgctggcgg cgctccccgc aaccgggccg tggtgtaggg gtattattaa ctggcggatt
    20101 tatcgtatgg ctggcgatgt tgtactggtt atcgccaata ctcgttgaat aactggaaac
    20161 gcattatgtc gcacgtagag ttacaaccgg gttttgactt tcagcaagca ggtaaagaag
    20221 tcctggcgat tgaacgtgaa tgcctggcgg agcttgatca atacatcaat cagaatttca
    20281 cgcttgcctg tgaaaagatg ttctggtgta aagggaaagt tgtcgtcatg gggatgggga
    20341 aatcggggca catcgggcgc aaaatggccg caacgtttgc cagcaccggt acaccttcat
    20401 ttttcgtcca tcctggtgaa gccgcgcatg gtgatttagg catggtcacc ccacaggatg
    20461 tggtgattgc tatctctaac tcaggtgaat ccagcgaaat cacggcctta attccagtgc
    20521 ttaagcgtct tcacgtaccg ttaatctgca tcaccggtcg cccggagagc agcatggcgc
    20581 gcgccgcaga tgtgcatctg tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg
    20641 caccgaccag cagcaccacc gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt
    20701 taaaagcacg cggctttact gctgaagatt ttgcgctctc acacccaggc ggcgcactgg
    20761 gtcgtaaact tctgctgcgc gtaaacgata ttatgcatac gggcgatgag atcccgcatg
    20821 ttaagaaaac ggccagtctg cgtaacgcat tgctggaagt tacccgcaaa aatcttggta
    20881 tgactgtcat ttgcgatgac aatatgatga ttgaaggcat ctttaccgac ggtgatttac
    20941 gccgtgtctt cgatatgggc gtggatgttc gtcagttaag tattgccgat gtgatgacgc
    21001 cggggggaat acgtgtacgc cctggcattc tggccgttga ggcactgaac ttaatgcagt
    21061 cccgccatat cacctccgtg atggttgccg atggcgacca tttactcggt gtgttacata
    21121 tgcatgattt actgcgtgca ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa
    21181 agcaggtgcg tcgcttgcga cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc
    21241 agagaacatt cgtctgctga tcctcgacgt tgatggcgta ctgtcagatg gcctgattta
    21301 tatgggcaat aacggcgaag agctgaaagc gttcaatgtt cgtgacggtt atggcattcg
    21361 ttgtgcgctc acctctgata ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt
    21421 agaagatcgt tgtgccacat tggggatcac tcacttgtat caggggcagt caaacaaact
    21481 gatcgccttt agcgatttgc tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt
    21541 tggcgatgat ctcatcgact ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc
    21601 cgatgcgcat ccactgttga tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg
    21661 tggcgcagtg cgcgaagttt gcgacttatt actcctggcg cagggcaaac tggatgaagc
    21721 caaagggcaa tcgatatgag taaagccaga cgttgggtta tcattgtgct atcactggcg
    21781 gttctggtga tgatcggcat taatatggcc gaaaaagacg ataccgccca ggtggtcgtc
    21841 aacaacaatg atcccaccta taaaagcgag catacggaca cgctcgtcta taacccagaa
    21901 ggggcactaa gctatcgatt gattgctcaa cacgttgaat attattccga tcaggccgtt
    21961 tcgtggttta cgcagccggt acttaccacg tttgataagg ataaaatccc gacatggtcc
    22021 gtaaaagcag ataaagccaa gctgaccaat gaccggatgc tctatttata tggacacgtt
    22081 gaagtcaacg cacttgtgcc agactctcaa cttcgcagaa tcacgacgga taacgcgcag
    22141 atcaatctgg tgacacagga tgttacctct gaagacctcg tcacgttata cggaacaaca
    22201 tttaactcca gcggtctgaa aatgcgcggc aacttacgca gcaagaacgc cgagctgatt
    22261 gaaaaggtta gaacatccta tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct
    22321 tgccagctca cttctggccg ccagcattcc ggcatttgcc gtaaccggag acactgatca
    22381 gccgatccac attgaatcgg accagcaatc tcttgatatg caaggcaacg tggttacctt
    22441 taccggtaat gtcatcgtca cccagggcac catcaaaatt aatgccgaca aagtggtcgt
    22501 tacccgtccg ggcggcgaac aaggtaaaga agtgattgac ggatacggta aaccggcaac
    22561 gttctaccag atgcaggaca acggtaaacc cgttgaaggt cacgcttccc agatgcacta
    22621 cgaactggca aaagattttg tcgttctgac gggtaatgct tatctgcagc aggtcgatag
    22681 caacattaag ggcgataaga tcacttacct ggtgaaagag cagaaaatgc aggctttcag
    22741 cgacaaaggc aagcgcgtaa ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa
    22801 caaaggccag accccggcac agaagaaggg taattaattc gttatggcaa cattaactgc
    22861 aaagaacctt gcaaaagcct ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt
    22921 caactccggg gaaattgtcg gtctgctggg gccaaacggt gccggtaaga ccaccacttt
    22981 ctacatggtt gtaggcattg tgccgcgcga tgcgggcaac atcattattg atgatgacga
    23041 tatcagtctg ctgcctctgc atgcacgcgc gcgccgcggt atcggctatc tgccacagga
    23101 agcctccatt ttccgtcgcc tcagcgttta cgataacctg atggcggtac tgcaaattcg
    23161 tgacgacttg tctgctgaac aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca
    23221 cattgagcac ctgcgtgaca gcatggggca gtcactctcc gggggtgaac gtcgccgtgt
    23281 agaaattgcc cgcgcactgg ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc
    23341 cggggttgac ccgatctcgg ttatcgacat taaacgcatc attgaacacc tgcgcgacag
    23401 cggcctgggc gtgctgatca ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg
    23461 cgcttatatc gtcagtcagg ggcatttgat cgcccacggc acgcctacag aaatcttaca
    23521 agacgaacac gttaagcgtg tataccttgg ggaagacttc agactctgat agggtagaag
    23581 tttgcgacgt tttagcagga gagtacgatt ctgaacatga agcaaggttt gcaactcagg
    23641 cttagccaac aactggcgat gacgccacag ctccaacagg caattcgtct gttgcagttg
    23701 tcgacgctgg aacttcagca ggagctacag caggcgctgg agagtaatcc gctgcttgag
    23761 caaatcgaca ctcatgaaga aatcgacacc cgcgaaacgc aagacagtga aacgctggac
    23821 accgccgacg cgctcgaaca aaaagagatg ccggaagagc tgccgctcga tgccagttgg
    23881 gacaccattt acaccgctgg tacaccatcc ggcaccagcg gtgactacat tgacgacgag
    23941 ctgccggtct accagggcga aacgacgcag accttgcagg attacctgat gtggcaggtc
    24001 gagctgacac cgttttccga cactgaccgc gctattgcta cctctatcgt cgatgccgtt
    24061 gatgaaaccg gttatctgac tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa
    24121 gagatcgaca tcgacgaggt tgaagccgtc cttaagcgga tccaacggtt tgatccggtc
    24181 ggtgtagcgg caaaagatct gcgtgactgc ctgctaatcc aactctccca attcgataag
    24241 accacgccgt ggctggaaga ggccagattg atcattagcg atcatctcga tctgttagcc
    24301 aatcacgact tccgcacttt aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa
    24361 gccgtcaatc tgatccagtc gctcgatccg cgccccgggc agtcgatcca gactggcgaa
    24421 cctgagtatg tcattccaga tgtgctggtg cgtaagcata acggtcactg gacggtagaa
    24481 ctcaacagtg acagcattcc gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat
    24541 aacgcgcgca acgatggtga cagccagttt atccgcagca atctgcagga tgccaaatgg
    24601 ttgatcaaga gtctggaaag ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt
    24661 gaacagcagc aagccttctt tgagcaaggt gaagaatata tgaaaccgat ggtactggcc
    24721 gatatcgccc aggctgtcga aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa
    24781 tacctgcata gtccacgagg catttttgaa ctgaagtatt tcttttccag tcacgtcaat
    24841 accgagggcg gcggcgaagc ttcctccacg gcgattcgtg cactggtgaa gaaattaatc
    24901 gcggcggaaa acccagcgaa accgttgagc gacagcaagt taacctcttt gctgtcggaa
    24961 caaggtatca tggtggcacg ccgcactgtt gcgaagtacc gagagtcttt atccattccg
    25021 ccgtcaaacc agcgtaaaca gctcgtttga cccaaccgat aaggaagaca ctatgcagct
    25081 caacattacc ggaaataacg tcgagatcac cgaggcactg cgcgaatttg ttacagctaa
    25141 atttgccaaa cttgagcaat attttgaccg aatcaaccag gtctatgttg ttctgaaagt
    25201 ggagaaagtc acccacacct cagatgcaac actgcatgta aacggtggtg aaattcatgc
    25261 cagcgcggaa ggtcaggaca tgtacgctgc cattgatggc ttaattgata agctggcacg
    25321 ccagttgacc aaacataaag ataaactgaa acaacactaa ttgtccgggc aattagcatg
    25381 tgcatggcgg cctgttgtgc ggcacaacgg gccatttgta cggttaatgc tccgagcctg
    25441 ttccactgtt tgagtgggca ggttcttagg tgaaattatg acaaataatg atacaactct
    25501 acagcttagc agtgttctta acagggaatg tacgcgaagc cgcgtccact gtcagagcaa
    25561 aaaacgcgcc ctggaaatca tcagcgagct ggcggcgaaa caacttagcc tgccgcctca
    25621 ggtggttttt gaagctatcc tgacgcggga aaaaatgggc agtaccggta tcggcaatgg
    25681 tattgccatt ccgcatggca aacttgaaga agatactctg cgtgccgttg gcgttttcgt
    25741 tcagctcgaa acgcctatag ctttcgatgc catcgacaac caaccggtgg acttactttt
    25801 tgccctgctg gtgccggcag accaaactaa aacgcacctg catactctgt cgctggtggc
    25861 gaaacgtctg gcggacaaaa ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga
    25921 gctgtatcaa atcattacgg ataccgaaag tactccggat gaagcgtagt tattcggtaa
    25981 tgtctctttt agacgttgtg aggagaaaca gtacatggta ctgatgatcg tcagcggacg
    26041 ttcaggttca ggtaaatctg tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt
    26101 ggataacctt cccgtagtgt tgttacccga tctggctcga actctggccg atcgcgagat
    26161 ttctgccgcc gtcagcattg atgttcgtaa tatgccggag tcaccagaaa tattcgaaca
    26221 ggcgatgagt aacctgcctg acgctttctc accgcaacta ctgttcctgg atgccgaccg
    26281 taatacctta attcgtcgtt acagtgacac gcgccgactg catccgcttt ccagcaaaaa
    26341 cctgtcgctg gaaagtgcta tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg
    26401 agcggatctg attgtcgata cctcagaaat gtccgttcac gagctggctg aaatgctgcg
    26461 tacccgtctg ctgggtaaac gtgaacgcga actgaccatg gtctttgagt ctttcggctt
    26521 caaacacggt atccctatcg atgcagatta cgtctttgac gtgcgcttct tgccgaaccc
    26581 gcactgggat ccgaaactgc gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct
    26641 cgaccgccac acagaagtac acaattttat ctaccagacg cgaagctatc ttgagctatg
    26701 gttacctatg ctggaaacca acaaccgtag ctacctgacg gtcgccattg gttgtaccgg
    26761 cgggaagcac cgttcggtgt atattgcaga gcaactggca gactacttcc gctcgcgcgg
    26821 taaaaacgtc cagtcacgcc atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa
    26881 actgttgaaa tcacaaacaa gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa
    26941 ttaatgcagg gttttgacgc tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa
    27001 gccaacagcg ttattgcgct gctgatgttg gattctgcca aaggacggca gattgaagtt
    27061 gaagcgaccg gtccacagga agaggaagca ctggccgccg ttatcgccct ctttaattct
    27121 ggttttgatg aagattaatc ttcatcactt tgacatacaa ctcccttcaa gctcccccga
    27181 caataagaaa atcacgtact gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg
    27241 cttttcactt tcagaagaac cttaagaaaa ccttaagagg cattgtttag gttttgttta
    27301 agttaatcga tcatactgga gatcgtcaga aaatatttcc aggagatggc atgattcgct
    27361 tatctgaaca aagtcccctg ggcaccggaa gacatcggaa atgttatgcg catccggaag
    27421 atgcccaacg ctgtatcaag attgtctacc atcgtggcga tggtggcgat aaagagatcc
    27481 gccgtgagtt aaagtactac gcgcatcttg gtcgccggtt aaaagactgg agtggaatac
    27541 cgcgctatca cggtaccgtt gagacggatt gcggaaccgg ttatgtctac gatgtgattg
    27601 ccgattttga cggcaagcct tccattacgc tgaccgaatt tgctgaacaa tgccgttacg
    27661 aagaagacat tgcacaactg cgccagctac tgaaacagct gaagcgttat ttgcaggata
    27721 accgtatcgt gacgatgtcg ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt
    27781 cagaggttat cccggtcgtc tgcgacaaca ttggtgaaag cacattgatt ccactggcga
    27841 cctggtcgaa gtggtgttgt ctgcgtaagc aagagcggtt atggaaacga tttatcgccc
    27901 aaccggcgct ggctattgcc ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg
    27961 ccctcacttc gcgcgaagct taatccagct ggtgttgttg cataaacggc tcactgccta
    28021 actgatacat ctgccgtaaa atccacgcct gacggctacg cacgtagccc gatggcgagg
    28081 agactttgaa acgaagtgga ttaggtaata cagctgccag taatgcagct tccgaccggg
    28141 taagtttgct cgcgggtttg tggaaataac gttgtgccgc agcttcgacg ccaaacacac
    28201 cgtcgccaaa ttcggcgata ttcaggtaaa ccgtcaggat acgctttttg ctccagactg
    28261 tttctatccc cagcgttaat ccagcttcca gcccttttcg tacccagcta cgcccatccc
    28321 ataaaaagag atttttggcc gtctgctgag aaatcgttga agcaccgcga atacggtttt
    28381 cattgcgctc gttgtgcgcc agggctttct caatggaagc gacatcaaag ccccagtgct
    28441 caggaaattt ctgatcttct gcggcaatca ccgccagtcc catccacggc gagatttgat
    28501 ccatactgac ccagtcagaa tgtgccacgt aacgaaaatt gccatgcagc caggcgctga
    28561 cctgtcgctc gaccattacc gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc
    28621 ccccgcccca gaaaacggcg aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac
    28681 taaacaccgt taagcggctt ttactcattc agccagaacc agcacgcggg aaaccagctt
    28741 atcaatgccg ctcgccgctt ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac
    28801 aatcttattg tcttcatcaa ccacgatatc atcgacagga cacggcacat gctccgcgcc
    28861 catctcttcc agcacttctg cggtatcgat atcagtaccg atggtcaaac gcagcgggaa
    28921 atcgaaaatt ttcggcagca tcgccggggc aatacacata aaaccaagcg gttttccggc
    28981 ctgatgcatc gcttgtgcca gctcctttaa ttcacggtca acggtgcatt cgctaccaag
    29041 actggcaaaa ttgcttaaat tcttcgccgc gccaaacccc cccggcacaa tcaacgcatc
    29101 cagttcagcg gcatcggcct gggccagagg acggatttca ccgcgcgtta ttcgtgccgc
    29161 ttcaatcagc acattgcgcg tttccgtcat cgcttcgcca gttaaatggt tgataacatc
    29221 aacctgctgc ttatccggtg caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa
    29281 caacgtcaac accgcttcat gaatttcaga accgtcatag acgccgcatc cgctcagaat
    29341 tacgccaatt ttcttcattg tgatcatcct ttcgcaattg actgaaacac attaattttt
    29401 ttaataaaaa tggtacgcat cacacattta actgattcat gtaacaaatc atttaagttt
    29461 tgctatctta actgcgtgcg gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat
    29521 aatttacggc gcagccaaga tttccctggt gttggcgcag tattcgcgca ccccggtcta
    29581 gccggggtca ttttttagtg gcttttgcca cccacgcttt cagcacttct acgtcgtgac
    29641 gccactcttc tttcatctct tcaatccatt caccgacgtt atcttcccag gcgggaaggt
    29701 caggagactg aatttgctga cccagctgtt gcagatggcg taaccccact gaccccgccg
    29761 cacctttaat tttatgtcct tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg
    29821 actccagcac gctgacatag cccggcatca tcttctcaaa caccgctaac ccgtcggtga
    29881 tcagcttcgg tcctacaagt tcgagatact gttccagcat gggaatatcg agcaatgctt
    29941 ctgatttact gttctcttct gtcgtcaccg tactctcctc atcatcctgg gtatcccaga
    30001 atttcttgat catcgcggtt agcgccggaa ccgaaagcgg cttactcagc acatcatcca
    30061 ttccagcatt gaggtactct tgtttgtctt tcagcacgtt agcggttaag gccaccagcg
    30121 gcggtaaatc ctcgcgcgga taacgcttcg tcagttcacg agagatatcc agcccggtca
    30181 tatctggcaa ctgaatatcc agcaacacca ggtcgtattc gcccggttta aacatctcca
    30241 gcgccgcctt gccggtcatg gcgacatcaa cgctgttacc taatttttcc agcacagaac
    30301 gcgcgacaat cacgttcagt tcaatgtctt ccaccagcag cacattcagc gccggtaaag
    30361 gcatatcgtc ttcatcaaac gcatcatcga cctcttctgc caccgacggt gcgtggatcg
    30421 tcaacgtaaa ggttgaacct ttgccctgtt cgctggtaac cgtaatatcg ccacccatat
    30481 ttttcgccag acgacgagaa acggccagac caataccggt gccggtggca ggtttaccgc
    30541 catgactgtc tttcacctgg taatacatgg cgaagatttt atccagctca tcctgcggaa
    30601 tgccgatacc agaatcttcc acttcaaaat gcagcatatc gccttcatcg tagcgcacgc
    30661 gcacggtaac ctggccttgc tgggtgaatt tgacggcgtt actgatgagg ttccacagga
    30721 tctgccgtaa acgcgtcccg tcggtaatga cctgatgcgg taatggcagc gtaggctcca
    30781 ggttaaagcg caatcctttt tgttgcgcct gcaatgcgga gagattttcc agatcggcaa
    30841 ggaagctggt gaaatcaacc ggctgattat caagctggac cttgcgccgt tccatcttat
    30901 ccatgtcgat aatatcgtta aagatattcc ccagcgtgac ggccgaaaca tggatagttt
    30961 tgagatattt ttcctgctcg gcggtgagtt cggtatccag cagaatgcgg ctcaggccga
    31021 cgataccgtt cagcggtgta cgcaattcgt gactgatggt ggaaataaac gtcgttttgt
    31081 cacggctggc gcgttcaagc gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa
    31141 agcccatcaa accgtgacgt ttacccacgc ggtcgtagta cggcacttta cggatttcaa
    31201 agcaggcttt gcgcccgtcc gggtaatcca gccactgttc ataggtcagt gacacattat
    31261 gacggaacac tttttcatcg gtttcaatga cttttgcggc ggcttccggt gagtaaacat
    31321 cagcaggttt caggtgaacc agttgttttt cgctttttcc ggtcagcagc tccatcgcgc
    31381 ggttacagcc ggaaaactct ttatcttcgt tacgataaaa aaccaggtcg ggtgaagcat
    31441 caaggaagga acgtaagaat gaggattgct gctcgagctg aatttgtgtc tcttcgcgct
    31501 ctttgatttc aattttcagt tggccgaagg tttcctgcag ttctgcttcc gctttttcac
    31561 gaacggcaat ttcctgattt agctgggcaa tattatcttt taactgaacg ttgaggctca
    31621 aatcgcgctc gcgcatctcc tccagttttt gcaccagccg tgacagacgt tgtcgtgact
    31681 cctccagttg ctcgacgacc accgatagaa agtagaccgc ccacggcgta atcagcaaac
    31741 caaagaagat agaacgaata acatcaatgc tttcgacctg accatgcagc accatggtta
    31801 ccgccatttg taccacaatg gcaagaacga cgagggccag cgccagcaac attgagaagc
    31861 gcaccagacc taacttcatc atcaggtcaa cataatactg cgccagcaga cgaatttgct
    31921 tcattaggga attccttcac gacaacctgc cccaataata cccaattctg acagttacgt
    31981 tataaattgt gtgagaaatg cggaattccc ctcaacgacc tactccgttg gaggtagcca
    32041 cggacgtccc agcgccgatc cctgtacgcc atgttcattc agatagcgat ccagctcgac
    32101 cattcccgtc catcgatttt cgcaccacag cggagcaagc agcgtcgggc gacgggcgct
    32161 ggcggaaatg cggtggtaga tcacttccgg tggcgtatgg cgaatcattt ctccggcagt
    32221 gagcgtataa tcctccagtt caataccgtt caaacgcccc gcttcccagg ctttcgccat
    32281 aatgctgcct ttcacaatat gcagcggatg cagctttatg ccgtccacgc cggtttcaac
    32341 cactcgctcc agcgtttgca aacattcagc ctgcccttcg ccaggcaggc cgacaattaa
    32401 gtgggaacac accttcagcc cgcgctgacg tgccagctgg gttgtacgct gataacaggc
    32461 aaaatcatgg ccgcggttga tgcgatgcag tgttttgtcg tgggcggttt gtagccccag
    32521 ctccagccac acttcgtaac cctggtcctt atattcgcaa agcagatcca gcaccgcatc
    32581 cggcacgcag tccgggcgga taccaacaca caaaccgaca atattggcct ggctcaccgc
    32641 ctgctgatac atagaacgca gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa
    32701 gtaggccaga tagcgtttag cgcggttaac taaattcgcc tggtgcgcca gttgctcggc
    32761 aatggaacga tgctgctgcg cttcatcggc aaacgaggca acattacaga atgtgcagcc
    32821 accacgcccg atggtaccgt cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt
    32881 atgcaccttt tgcccataac gacgggtgag atcaccacca aacatattga ctaatttctg
    32941 taactgcata atctgataga ccgcgccttg aaaagaggcc aaagcctgcc atttttagct
    33001 caattcggcg atgacctgga tcaatcgtcc cggcctgctt ttatcaactg cataatcaat
    33061 caaaattacc gaaatttcat gcataatcac ataaatcact tttgcttatc ttgtgtcaga
    33121 tttttttatc tccttatgga ttttaggcaa aaacagtagc atgaaacgtc attaccaatt
    33181 aaggcagtat aaaatgctgg ttttctcgtc agttcaaggc aggataaggg ttaacacgcc
    33241 tttatgacag tcaggaattg actgtttctc taacgactct ccttttagcc ttaaagataa
    33301 aatctatttt aatttcagct atttaataaa gaattttgcg ctaaagcaca tttctgtacc
    33361 aataagcttg ccatttgacc tgtatcagct ttcccgataa gttggaaatc cgctggaagc
    33421 tttctggatg agcagcctgc tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg
    33481 tattaaccga tgcgaaaagg acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca
    33541 aaccccgtcg ccacgctctt tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc
    33601 agagcctagg ggaggttcac gatatgttgt acgataaatc ccttgagagg gataactgtg
    33661 gtttcggcct gatcgcccac atagaaggcg aacctagcca caaggtagtg cgtactgcaa
    33721 tacacgcact ggcccgcatg cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg
    33781 acggttgcgg cttgctgtta caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc
    33841 gcggctggcg tttagcaaaa aactacgctg tcgggatgct cttcctgaat aaagatcctg
    33901 aactcgccgc tgccgcacgc cgcatcgttg aagaagaact gcaacgcgaa accttgtcga
    33961 ttgtgggctg gcgtgatgtc cccactaacg aaggcgtgct gggtgaaatc gccctctcct
    34021 ctctgccacg cattgagcaa atttttgtga acgccccggc aggctggcgt ccacgcgata
    34081 tggagcgccg tctgtttatc gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag
    34141 acttctacgt ctgtagcctg tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg
    34201 cggatctgcc gcgcttttat ctggatcttg cggacctgcg tctggaatcg gccatttgcc
    34261 tgttccacca gcgcttctcc actaacaccg taccgcgctg gccgctggcg caaccgttcc
    34321 gctatctggc gcataacggt gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg
    34381 cgcgtaccta taaattccag acgccgctga tccctgacct gcacgacgcc gcgccattcg
    34441 tcaacgaaac cggttctgac tccagttcca tggataacat gctggaactg ctgctggcag
    34501 gcgggatgga tatcatccgc gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc
    34561 cggatatgga cccggaactg cgtgccttct ttgactttaa ctccatgcat atggagccgt
    34621 gggatggtcc ggcgggcatc gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc
    34681 gtaacggtct gcgtccggca cgctacgtca ttaccaaaga caagctcatc acctgcgcct
    34741 ctgaagtcgg tatctgggat taccagcctg acgaagtggt cgaaaaaggc cgcgtcggac
    34801 caggcgaact gatggtgatc gacactcgca gtgggcgtat tctgcactcg gcagaaaccg
    34861 atgacgatct gaaaagccgc cacccttata aagagtggat ggagaaaaac gtccgccgac
    34921 tggtaccgtt tgaagatctg cccgatgaag aagtgggtag ccgcgaactg gacgacgaca
    34981 cgcttgccag ctaccagaaa cagtttaact acagcgcgga agagctggac tccgtaattc
    35041 gcgtactggg cgaaaacggt caggaagcgg tcggttcgat gggcgatgat accccattcg
    35101 ccgtgctctc cagtcagccg cgcattattt acgactactt ccgccagcag tttgcccagg
    35161 tgactaaccc gccaatcgac ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta
    35221 tcggtcgtga aatgaacgtc ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta
    35281 aatcgccgat tctgctctac tccgatttca aacagctcac gacgatgaaa gaggagcact
    35341 accgcgcaga tacgctggat atgacctttg acgtcactaa aaccacgctc gaagcgacag
    35401 tcaaagagct gtgcgacaaa gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc
    35461 tctccgaccg gaatatcgct aaagatcgcc tgccggttcc ggctccgatg gcggttggcg
    35521 cgatccagac ccgtctggtc gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa
    35581 ccgccagcgc ccgcgatccg caccacttcg ccgtgttgct gggtttcggc gcgacggcta
    35641 tttatccgta ccttgcctat gaaacgctgg gccgcctggt agacacccac gcgattgcca
    35701 aagattatcg taccgtgatg ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa
    35761 tcatgtccaa aatgggcatc tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag
    35821 cggtcggtct acacgatgat gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg
    35881 gtggggcaag ctttgaagac ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc
    35941 tggcgcgcaa gccgattagc cagggcggcc tgctgaaata cgtccacggc ggcgaatacc
    36001 acgcctacaa cccggacgtg gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca
    36061 gcgactatca ggaatacgcg aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc
    36121 tgctggcaat tacgccgggt gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg
    36181 aactgtttaa acgctttgat accgccgcga tgtctatcgg cgcgttaagc ccggaagccc
    36241 acgaggcgct ggcggaagcg atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg
    36301 gcgaagaccc ggcgcgctat ggcactaaca aagtgtcgcg catcaagcag gtggcttctg
    36361 gtcgcttcgg cgttactccg gcgtatctgg tcaatgccga cgtcattcag attaaagtcg
    36421 cccagggcgc gaagccgggc gaaggcggtc agttgccggg cgacaaagtc actccttaca
    36481 tcgccaaact gcgctattcg gtgcccggag tgacgctgat ctccccgccg ccgcaccacg
    36541 atatctactc tatcgaagac ttagcgcagc ttattttcga cctcaagcag gttaacccga
    36601 aagcgatgat ctccgttaag ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg
    36661 tggcgaaagc ttatgcggac ttgatcacca tcgcgggcta tgacggcggc accggcgcaa
    36721 gcccgctgtc gtcagtgaaa tatgcaggct gcccgtggga actcggtctg gtggaaaccc
    36781 aacaggcgct ggttgccaac ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc
    36841 tgaaaacggg tgtcgatatc atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg
    36901 gcactggccc aatggtggcg ctcggctgta aatatctgcg tatttgccac ctgaacaact
    36961 gcgccacggg tgtagcaact caggatgaca aactgcgtaa gaatcactat cacggcctgc
    37021 cgttcaaggt gactaattac tttgagttta tcgcccgtga aacccgcgag ctgatggcac
    37081 agctgggagt aacgcgtctg gtggatctga ttggtcgcac cgacctgctg aaagagctgg
    37141 acggtttcac cgccaaacag cagaaactgg cgctgtcgaa gctgctggag actgccgagc
    37201 cacatgcagg taagtcactc tactgcactg aaaacaaccc gccgtttgat aacggcctac
    37261 tgaacgcgca gttgctgcaa caggcgaaac cgtttgtcga tgaacgccag agcaaaacct
    37321 tctggttcga tatccgcaac accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg
    37381 cccagacgca cggcgatcag gggctggcag ccgatcctat caaagcgtac ttcaacggca
    37441 ccgcaggcca gagtttcggt gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg
    37501 atgccaacga ctatgtcggt aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg
    37561 ttggttccgc cttccgcagc catgaagcaa gcattatcgg caacacctgc ctgtatggcg
    37621 cgaccggtgg tcgtctgtat gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact
    37681 ccggtgctat caccgtggta gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg
    37741 gtattgtctg tattctgggt aaaaccggcg ttaacttcgg cgcgggcatg accggcggct
    37801 tcgcttacgt tctcgatgaa agcggtgatt tccgcaaacg cgttaacccg gaactagtcg
    37861 aagtcttaag cgtcgacgat ctggcgatcc atgaagagca tctgcgcggt cttatcaccg
    37921 agcatgttca gcataccggt tctcagcgcg gtgaagagat tctggcgaac tggtcaacct
    37981 tcgccacgaa attcgcgctg gttaaaccga agtccagtga tgtaaaagca ctgctgggtc
    38041 accgtagtcg tagcgcagca gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga
    38101 gtcagaatgt ttatcaattt atcgacctgc agcgcgttga tccgccaaag aaaccgctga
    38161 agatccgtaa aattgagttt gttgaaattt acgagccgtt ttccgaaggc caggccaaag
    38221 cgcaggctga ccgctgtctg tcgtgcggca acccatactg cgagtggaaa tgcccggtac
    38281 acaactacat cccaaactgg ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag
    38341 aactgtcgca tcagaccaac acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc
    38401 gtctgtgcga aggttcctgc accctgaacg atgagttcgg tgcggtgacc atcggcaaca
    38461 ttgagcgtta tatcaatgat aaagcgttcg aaatgggctg gcgtccggat atgtccggcg
    38521 tgaagcaaac cggtaaaaaa gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg
    38581 cggatgtcct gacgcgtaac ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg
    38641 gcgggctgct gaccttcggt attccggcct tcaagctgga aaaagaggta atgacgcgtc
    38701 gccgtgaaat cttcaccggc atgggtattg aattcaaact caataccgaa gtgggccgcg
    38761 acgtacagct ggacgatctg ctgagtgatt acgatgccgt gttccttggc gttgggactt
    38821 atcagtcaat gcgcggcggg ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc
    38881 cgttccttat cgccaacacc aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt
    38941 tcgtcagcat ggaaggcaaa cgcgtggtag tacttggcgg tggcgacact gcgatggact
    39001 gcgtgcgcac gtctgtacgc cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg
    39061 aagagaacat gccgggttcc cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt
    39121 tcaaattcaa cgtccagcca ctggggattg aagtgaacgg taacggcaaa gtcagcggcg
    39181 taaaaatggt acgtaccgaa atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga
    39241 tcgttgcagg ttccgaacat atcgttccgg cagatgcggt gatcatggcg tttggtttcc
    39301 gtccacacaa catggaatgg ctggcaaaac acagcgtcga gctggattca caaggccgca
    39361 tcatcgctcc ggaaggcaac gacaacgctt tccagaccag caacccgaaa atctttgctg
    39421 gcggcgatat cgtccgtggt tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag
    39481 cggcagacgg cattatgaac tggctggaag tttaagctct gtcccagcgc cgcctcctca
    39541 gaaaagagtc ggcgctgaaa ttctcctttc tttctggaaa tcgcctcgca taatctgaag
    39601 aaaaccaatg taatacgctc atctggattg ttaccggtag atgcccggcg tatagctgat
    39661 tccattattc aacgcagcgc catataaagg gacagataat ggaatcgctc tcagagagaa
    39721 cctcaacagg ctaccagcaa atccacgacg gcattattca tctggtcgat agcgcccgaa
    39781 cggaaacggt acgtagcgtt aacgcgctga tgaccgcgac gtactgggaa attggccgac
    39841 ggattgtcga atttgaacaa ggtggtgagg ccagagcagc ttatggtgca cagttgattg
    39901 atcggctatc gaaggattta agtcgacggt ataaacgtgg ggtctctgca aaaaacttac
    39961 gccaaatgag gcttttttac ctcttttttc aacatgttga aattcgccag acagtgtctg
    40021 gcgaattaac accattggga attccgcaga caccatctgc agaattccca tcggcaaaga
    40081 tttggcagac gctgtctgcc aaattgagag gctgtttgat atttccacag cttttgtatt
    40141 gcatctccta ttttcggttt gtatattcat gcaaataaac gaagg
//
LOCUS       DADLSE010000022        38023 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLSE010000022 DADLSE010000000
VERSION     DADLSE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.022"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN692_001147"
     CDS             complement(21..1019)
                     /gene="fbp"
                     /locus_tag="JN692_001147"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7263511.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1195..2568
                     /gene="mpl"
                     /locus_tag="JN692_001148"
     CDS             1195..2568
                     /gene="mpl"
                     /locus_tag="JN692_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7263512.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2651..3202)
                     /locus_tag="JN692_001149"
     CDS             complement(2651..3202)
                     /locus_tag="JN692_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7263513.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3296..4648
                     /gene="pmbA"
                     /locus_tag="JN692_001150"
     CDS             3296..4648
                     /gene="pmbA"
                     /locus_tag="JN692_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7263514.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKVPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4831..5217
                     /gene="cybC"
                     /locus_tag="JN692_001151"
     CDS             4831..5217
                     /gene="cybC"
                     /locus_tag="JN692_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7263515.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLADNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN692_001152"
     CDS             complement(5262..5726)
                     /gene="nrdG"
                     /locus_tag="JN692_001152"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7263516.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN692_001153"
     CDS             complement(5884..8022)
                     /gene="nrdD"
                     /locus_tag="JN692_001153"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7263517.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFAGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN692_001154"
     CDS             complement(8416..10071)
                     /gene="treC"
                     /locus_tag="JN692_001154"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7263518.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPCFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNGAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN692_001155"
     CDS             complement(10121..11542)
                     /gene="treB"
                     /locus_tag="JN692_001155"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HAY7263519.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN692_001156"
     CDS             complement(11661..12608)
                     /gene="treR"
                     /locus_tag="JN692_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7263520.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12793..12846
                     /gene="mgtL"
                     /locus_tag="JN692_001157"
     CDS             12793..12846
                     /gene="mgtL"
                     /locus_tag="JN692_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7263521.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12987..15683
                     /gene="mgtA"
                     /locus_tag="JN692_001158"
     CDS             12987..15683
                     /gene="mgtA"
                     /locus_tag="JN692_001158"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7263522.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN692_001159"
     CDS             complement(15889..16275)
                     /gene="ridA"
                     /locus_tag="JN692_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7263523.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN692_001160"
     CDS             complement(16348..16809)
                     /gene="pyrI"
                     /locus_tag="JN692_001160"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7263524.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN692_001161"
     CDS             complement(16822..17757)
                     /gene="pyrB"
                     /locus_tag="JN692_001161"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7263525.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN692_001162"
     CDS             complement(17761..17895)
                     /gene="pyrL"
                     /locus_tag="JN692_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7263526.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18176..18571)
                     /locus_tag="JN692_001163"
     CDS             complement(18176..18571)
                     /locus_tag="JN692_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7263527.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN692_001164"
     CDS             complement(18703..19416)
                     /gene="bdcA"
                     /locus_tag="JN692_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7263528.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGTLDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPIRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19487..20080
                     /locus_tag="JN692_001165"
     CDS             19487..20080
                     /locus_tag="JN692_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7263529.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20225..20677
                     /locus_tag="JN692_001166"
     CDS             20225..20677
                     /locus_tag="JN692_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7263530.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20800..21617
                     /locus_tag="JN692_001167"
                     /pseudo
     CDS             20800..21617
                     /locus_tag="JN692_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21732..22517
                     /locus_tag="JN692_001168"
     CDS             21732..22517
                     /locus_tag="JN692_001168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263531.1"
                     /translation="MINLSHILIDSLDDAKVNLTPVIHSMLVTFLELPYNNDVKILEW
                     CFNKSMQYFDDSAKIEHACSVINHINFLCDQSKVAETLFFNLDKEPYKNSPELQELIW
                     KKLVVYVNDFNLSNREKTYLIQRIFNNVESLFNKVPVSILVNDILLNDFFMKNTEMIN
                     WYFPRLLKSYEGEKNYFDNLKYDINDEKSNKEILKNQPDNVIKEKLNNEFKLRFRMMQ
                     TIFKSKVNVSPYIDQQRLNTLNPPENLRMAIEKFGWKKKTITA"
     gene            complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN692_001169"
     CDS             complement(22570..23583)
                     /gene="argF"
                     /locus_tag="JN692_001169"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7263532.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
     gene            23745..24161
                     /gene="rraB"
                     /locus_tag="JN692_001170"
     CDS             23745..24161
                     /gene="rraB"
                     /locus_tag="JN692_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7263533.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(24207..24710)
                     /locus_tag="JN692_001171"
     CDS             complement(24207..24710)
                     /locus_tag="JN692_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7263534.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            24903..26099
                     /locus_tag="JN692_001172"
     CDS             24903..26099
                     /locus_tag="JN692_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7263535.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMK
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN692_001173"
     CDS             complement(26153..29008)
                     /gene="valS"
                     /locus_tag="JN692_001173"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7263536.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENHESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN692_001174"
     CDS             complement(29008..29451)
                     /gene="holC"
                     /locus_tag="JN692_001174"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7263537.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN692_001175"
     CDS             complement(29585..31096)
                     /gene="pepA"
                     /locus_tag="JN692_001175"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7263538.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRARFNGEE"
     gene            31363..32463
                     /gene="lptF"
                     /locus_tag="JN692_001176"
     CDS             31363..32463
                     /gene="lptF"
                     /locus_tag="JN692_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7263539.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            32463..33545
                     /gene="lptG"
                     /locus_tag="JN692_001177"
     CDS             32463..33545
                     /gene="lptG"
                     /locus_tag="JN692_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7263540.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(33664..35166)
                     /locus_tag="JN692_001178"
     CDS             complement(33664..35166)
                     /locus_tag="JN692_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7263541.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN692_001179"
     CDS             complement(35295..36314)
                     /gene="ahr"
                     /locus_tag="JN692_001179"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7263542.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            36510..36594
                     /locus_tag="JN692_001180"
     tRNA            36510..36594
                     /locus_tag="JN692_001180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36544..36546,aa:Leu,seq:caa)
     gene            36793..38023
                     /locus_tag="JN692_001181"
                     /pseudo
     CDS             36793..38023
                     /locus_tag="JN692_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         9137 a         9445 c         9796 g         9645 t
ORIGIN      
        1 gtgtggagag gcgcaggaga ttacgcgtcc gggaactcac ggataaagcg ttcgacatct
       61 tcaaccatat ggtcgttgcc gacaaagaac gaacggcgct ggtgcagagt ttccgggatg
      121 atatccagaa tacgctcttt gccatcgctc gctttaccgc ccgcttgttc cgccaggaat
      181 gccatcgggt tgcactcata aagcaaacgc agtttgccgt ccgggtggct ggcggtgctt
      241 gggtagagat aaataccgcc tttcagcagg ttacggtgga aatccgcaac cagtgaaccg
      301 atataacgcg aggtatacgg gcgattggtg gatttatctt cttcctggca gaatttaatg
      361 tacttcttca ccccgttcgg aaacttaatg tagtttcctt cgttgatgga gtaggttttg
      421 cctttctccg ggaagcgcat ccgttcctgg cacaggcaga aaacgcccag cgaaggatcg
      481 taagtaaagg cgtgaacacc gcatccggtg gtgtaaacca gcatggtaga ggagccgtat
      541 accacgtaac ctgccgcaac ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc
      601 ggcgtgccaa caggcgtaac gcggcggtag atggagaaaa tggtaccgac agagacgtta
      661 acatcgatgt tggacgagcc atccaggggg tccatcagca ccacgtattt tgcgtgttca
      721 cagccttcaa agacgacaat ctcatcttct tcttcagagg caatccccgc aacgatatcg
      781 cgtgctttca gtgcggcttt cagtttttca ttagcgaaca agtcgagttt ttgctgaacc
      841 tcgccctgca cgttctcagc accgctggca cccaggatat caaccagtcc tgctttgttg
      901 atatcgcgat ggataatctt ggcgcccagt tttattgccg acagcaaagc agtgagctca
      961 ccggtagcat gagaaaactc gtgctgcttt tcgacaataa attcacctaa cgttttcata
     1021 aaactttccc tgcaatgttt atggagtaaa gcgaccgcaa caatcttaac aaataatctc
     1081 aatgttgcgc tcaggtgaat cgcgccagca aattgcggat tatcctgaaa tgcgtttctc
     1141 acttgcccga catatgcgta aaatgagcgg cagattaaaa aaggatagtg acgtatgcgc
     1201 attcatattt taggaatttg tggcacattt atgggcggtc tggcgatgct ggcgcgccag
     1261 ttaggccatg aagtaacggg ttcggacgcc aatgtgtatc cgccgatgag caccttactt
     1321 gagaagcaag gcattgagct gattcagggt tacgatgcca gccagctcga gccgcagccg
     1381 gatctggtga ttattggcaa cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg
     1441 gaaaaaaaca tcccttatat gtcaggtcca cagtggctgc acgattttgt gctgcgcgac
     1501 cgctgggtgc tggccgttgc cggtacacac ggcaaaacca ccaccgcggg aatggcgacc
     1561 tggattctgg aacagtgtgg ttacaaaccg ggatttgtga tcggcggtgt gccggggaac
     1621 tttgaggttt cggcgcgtct gggcgaaagc gacttctttg ttatcgaagc ggatgagtac
     1681 gactgcgcct tcttcgacaa acgctctaaa tttgtccatt actgcccgcg tacgctgatc
     1741 ctcaacaacc ttgagttcga tcacgccgat atctttgacg acctgaaagc gatccagaaa
     1801 cagttccacc atctggtgcg tatcgttccg gggcagggcc gtattatctg gccggaaaat
     1861 gacatcaacc tgaaacagac catggcgatg ggctgctgga gcgagcagga gctggtgggt
     1921 gagcagggtc actggcaggc gaaaaagctg accaccgatg cttccgaatg ggaagttttg
     1981 ctggatggcg aaaaagtggg cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac
     2041 aatggcctga tggcgattgc agcggctcgc catgttggtg tagcgccggc agatgccgct
     2101 aacgcgctgg gttcgtttat taatgctcgt cgccgtctgg agttgcgtgg tgaagcgaat
     2161 ggcgtcacgg tatatgacga ttttgcccat cacccgacgg cgattctggc aacgctggcg
     2221 gcgctgcgtg gcaaagttgg tggtacggcg cgcattattg ctgtgctgga accgcgctcg
     2281 aataccatga aaatggggat ctgcaaagac gatctggcac cttcattagg tcgtgccgat
     2341 gaagtcttcc tgctgcaacc ggcgcatatt ccgtggcagg tggcagaagt ggcagaagcc
     2401 tgcgttcagc ctgcacactg gagtggcgat gtggatacgc tggcagatat ggtggtgaaa
     2461 accgctcagc ctggcgacca tattctggtg atgagcaacg gcggttttgg tgggatccat
     2521 cagaaactgc tggatggtct ggcgaagaag gcggaagctg cgcagtaatt cggcttcagc
     2581 ctgatatagc attgccggat aaggcgttta cgccgcatcc ggcaaaccat actcacatca
     2641 acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa atctggcgtg
     2701 cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc agtgtacgca
     2761 gttgctgacg atcggcatcg ggccacagat tcaatacttc agcgatggcg tcatcacctt
     2821 gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc tgctggttgt
     2881 gacggttttt cagcttatcc agcgcctgac gaataggctc tacgtcgcgc tggcgtagca
     2941 tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc tgggcgagct
     3001 caatagccgg gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt ttccccagat
     3061 caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt tcacttttac
     3121 tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg tcgtcgagcc
     3181 agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat gttaccagtt
     3241 aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct acattatggc
     3301 acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg aagaagcagt
     3361 ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg ccgtcagcaa
     3421 gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg aattcaatag
     3481 tgatggcgcg ctggggatca ctgtttatca ccagaaccgt aaaggtagcg catcgtctac
     3541 cgatttaagc ccgcaggcca ttgcccgcac cgtacaggcg gcgctggata ttgcccgtta
     3601 tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct ttgacgcacc
     3661 gaatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg aactggcggc
     3721 ccgcgcagaa caggcggcat tgcaggcgga caaacgcatc accaataccg aaggtggcag
     3781 ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt tgcagggtta
     3841 ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg gcgatatgga
     3901 gcgtgattac gcctacacca ttggccgcgc gatgagcgat ctgcaaacgc cagagtgggt
     3961 cggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac tctccaccat
     4021 gaaagtgccg gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc atctggtggg
     4081 ggcgatagcg ggtggctcgg tttaccgtaa atctaccttt ctgctggatt cgctgggtaa
     4141 acaaattctg ccggactggc tgaccattga agagcatccg catctgctga aagggctggc
     4201 gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta ttaaagatgg
     4261 catcctgact cagtggctgc tgaccagcta ctcggcgcgg aaactggggc tgaaaagcac
     4321 cggacatgcg ggcggtattc acaactggcg gattgccgga caagggctaa gcttcgaaca
     4381 aatgctcaaa gagatgggca ccgggctggt ggtgacggag ctgatgggcc agggcgtgag
     4441 tgccattacc ggtgattatt cccgtggtgc agcgggcttc tgggtagaga atggcgaaat
     4501 tcagtatccc gttagtgaaa tcaccatcgc aggtaattta aaagatatgt ggcgcaatat
     4561 tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt ctgtgctgtt
     4621 gccggagatg aaaatcgccg ggcagtaaaa aactggcgcg acctgccgcg ctataaacta
     4681 aaaaattaca caaaatcatt cgcactgcat cgagacggaa agtgaatgag ggccggggag
     4741 cgtactcgca gtacgtgacc cgggcgaatg agcgtagccg tcgatgaggc agtgtgaagg
     4801 atgaagtgta aataaaaaag gaagtgagca atgcgtaaaa gcctgttagc tattcttgca
     4861 gtctcctcgt tggtattcag ttctgcgtcg tttgccgctg atcttgcaga caatatggaa
     4921 accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca agtcaaagac
     4981 gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc gccgaagctc
     5041 gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt cgacattctg
     5101 gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa agaagcgcag
     5161 gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta tcgttaattc
     5221 ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa atgatgcacc
     5281 acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg cacaaatttg
     5341 ccgtcgacca gcacgttaat caaatcaaca acctgcattt gcgcagcgtt gagttcgtcg
     5401 agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg gatgcgtttt
     5461 accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc ggagagggaa
     5521 atcccctggc gtttgatacg agtgtcattc agatctttaa tgatcttgtc ttccattgct
     5581 ttggtaaatg gctgaccaga atttacccgc catgtgcttt tgttatagca accggggcat
     5641 tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt gacgatgtcg
     5701 acaggatagt actgatgata attcattatt tcgcctggct tatcagtccg cgtgtaggct
     5761 gcatccggta atcaatgcct gatgcgacgc tgtcgcgtct tatcaggcct acagccgttg
     5821 ccaaatgtag gccggataag gcgtttacgc cgcatccggc atttgcttag cgcagagtga
     5881 agattaacct atctgcccat tccccaaatg tttaacgcgg cgcttaactt cttcctgctt
     5941 accagcgtta aacggacgtg catccgggct acctaaatat ccgcacacgc ggcgagttac
     6001 cgacacacgg gaggcgtcat ggttaccaca tttcgggcaa gtgaagcctt tgctggtgca
     6061 ctcgaactca ccggtaaagc cacactcgta gcactcatca atcggtgtat tggtgccgta
     6121 atacggtaca tgctgatagc tgtaatccca gacatcttcc agcgctttca ggttgtgctg
     6181 aatgtttgga tactcgccgt agcaaatgaa accaccgttc gccagcggcg ggtaaggcgc
     6241 ttcaaagtcg atcttgtcgt acgggttcac cttcttctcc acatcaaggt ggaaactgtt
     6301 ggtgtagtaa cctttatcgg tcacgcccgg caccacgcca aactcagcag tatcgagacg
     6361 gcagaagcgg tcgcacaggt tttcactcgg cgtgctgtag agactgaaac cataacccgt
     6421 ttcttctttc cactgatcca ctgcctgacg cagacgttca acaatggcga tacctttcgc
     6481 gcgaagcttc tcgttgtcgt aaacatgctc gccggcgaac agcgcgttaa tggtttcgtg
     6541 gatgccgatg taacccagcg aaatagacgc acgaccgttt ttgaagattt cagaaacatc
     6601 atcgtcagca ttcagacgta cgccacaagc accttccata tagaggatcg gggcgacgcg
     6661 cgctttcacg ccttcgagac gagcgatacg ggtcatcagt gccttacgtg ccagcaccag
     6721 acgttcatcc agcagcttcc agaaggtggc ttcatcgcct tttgcttcca gagcaatacg
     6781 cggcaggttc aggctgatca cgccgaggtt gttacgacca tcgtggatct gctcgccgtt
     6841 ttcattttcc cacacgccga ggaagctgcg gcagcccatc ggggttttaa acgaaccggt
     6901 gactttcact acctgatcgt agttcaggat atccggatac atgcgcttgc ttgcgcactc
     6961 cagcgccagc tgtttgatgt cgtagttcgg atcgcctttt ttatggttca ggccatcgcg
     7021 aatcgcaaac accagtttcg ggaacaccgc agttttacgg tttttaccga ggcctgcgat
     7081 acggttacgc aggatcgatt cctgaatcag gcgcgattcc cagctggtgc ccagaccaaa
     7141 accaaaggtt acaaacggcg tctgaccgtt ggcggtgtgc agggtgttta cttcgtactc
     7201 cagcgactgg aaggcatcgt agcactcttt gatggtacga gagttagcgt agccttcggc
     7261 gtccgggatg ttccactctt ctgcggtctt gcgatgtttg ttgtagctgg cggtaacaaa
     7321 cggcgccagc acttcatcga tacggttaat ggtggtgccg ccataaatat ggctggcgac
     7381 ctgagcaata atctgcgcag ttaccgcggt tgccgtagag atcgacttcg gcggttcaat
     7441 ctcggcgttc cccattttaa agccctgggt cagcatgcct ttcaggtcga tcaacatgca
     7501 gttgaacatc gggaagaacg gcgagtaatc gagatcgtga tagtgaatat cgccacgctc
     7561 atgtgcctgc accacgtcac gcggcagcag gtgctggcgt gcatagtgtt tagccacgat
     7621 cccggccagc aggtcgcgct gggttggaat caccttgctg tctttgttgg cgttttcgtt
     7681 gagtaacgag gcgttggtct gctcgaccag accacggatc tcctggttca ggcgaccgcg
     7741 tttttcacgt tcaatgtcgc gatcgtgacg gtactcgatg taagcacgag ccagttgttt
     7801 gtatggaccc gacatcagct ggttttcaac tgcggtctgg atctcgttga tatccacctg
     7861 gttgcggccc tgcatctgct cgctgacaac cgcggcaaca gtggcgcaat aatcggcatc
     7921 atcgacttcc gctgctttag ctgcacgcag aatcgcttct ttgatgcgct ctgatttaaa
     7981 cggcactttg cagccgtctc gtttcatcac atgcggtgtc atgatcgctc catattttta
     8041 agaacaggtt atccacagaa attgggaaag gcgttttccg gttttttcgt ttcgttttcc
     8101 ggtgctttcc gcaatcgcca tccgcttatc cacaacgccg cacctttttc gtggggagct
     8161 gtagtagcaa ttatagtcga ttaatacaac atattgggtt gggacgcatt ttaaagtcta
     8221 tatatagtgc tttgcatcaa ggatgtttta actttttttg atgtagctca aagtaaaaag
     8281 cagagcgtac ggatgacggg cgctacagcg ttatgtaaat tttttaatga atttgctggt
     8341 tgaaaaatca acaaaaacaa catgctgaca gacaaaaccc cggaatgacc ggcattatcg
     8401 gggcttaggg aagatttact tctgtaacca ccagacagcc tcaaaaggcc gtaaattcat
     8461 ggcacagggt tgtggtgagg cttcttcgta gttatgcatc acaagctgcc agttgccgcg
     8521 catttgccct ggctgccagg gttggatctc acggctaagg ttggcaatga ccagcaaggt
     8581 ttgccccttc cattcacggc gatagcacca caatacaggg ctgtttggca gcagatcctg
     8641 gtaattgccc catgtcagga tggcttcttg cttacgcagt gcgattaact tttggtaggt
     8701 gtaaaacaca gaggagtcat cggccagcgc ggcttctacg ttgatttgtt gatagttatc
     8761 gcctaggcca atccacggtt cgccagccgt aaacccggca ttatcgccgt tgctccattg
     8821 catgggcgtg cggctgttgt cacgggattt actggcgagg attgccaata actcgtcggc
     8881 atcacgacca tcgttgcgca gctcggcaaa catattgagg ctctccacgt cgcgatagtc
     8941 agtaatgcgc gtgaaatgcg ggttggtcat gccaatctct tcgccctggt agatatacgg
     9001 cgttccctgc atgccatgca gcaccatcgc cagcattttt gccgcaggca cgcggtattc
     9061 accttcatca ccaaagcgag aaacaatgcg cggctgatcg tggttacacc agaacaaggc
     9121 attccatgcc ccgttgtgca ttccttgttg ccagtggcgg aacaatgttt tcaacgccac
     9181 aaagtcaggt ttagccagcg tccatttttc accaccggga taatcgacct tcaggtgatg
     9241 aaaattaaag gtcatcgaca attcactgcc tgtcagtgcc gcgtatcgct ggcaatgctc
     9301 aaggctggtg gaggacattt cacctacggt cattaacccg cgtggcgtaa acacatcgcg
     9361 gttcatctcg tgcaaaaact cgtgtgctcg tggcccgtcg gtgtagaagc gacgcccgtc
     9421 gccatccagg tcttcaggga aacacgggtc tttggagatc agattcacca catccaggcg
     9481 caacccgtcg accccacgat cggcccagaa ttcacagact tttttcagct ctgcgcgtac
     9541 cgctggattc tcccagttga gatccgcctg ttctggtgca aagagatgca aataatactg
     9601 ttcgctttcc gcatgccagc gccacgcact accgccaaat tttgaacgcc agttgttcgg
     9661 tggcgtttct ggttcgccat cgcgccagat ataaaactgg cggtaagggc tttctttgtt
     9721 cagcgcctcg cgaaaccagg catgttgggt agaggtatgg ttaaacacca tatcgagaat
     9781 gatacgaatc ccgcgcgatt ttgcctgcgt caccagttcg tcaaaatcgt ccagcgtgcc
     9841 gtaggtggga tcaatcgccg tatagttcgc tacatcgtaa ccgttatcga cctggggaga
     9901 gacataaaag ggggttagcc agatggcatc aacgcccagt ttatgcagat agtccaggcg
     9961 ttggataacg ccacgtaaat cgccggtgcc gctacccgtg gtgtcctgaa aactctttgg
    10021 ataaatctgg tagataacgc cgttttgcca ccagtgggga agattagtca ttacgttatt
    10081 cctgcaaatg cgagggggcg caattgcgcc ccgaagaaaa ttaaacaatg tccagcgtgc
    10141 ccaggcggta tttccgctga tagataaacg aggtgagtac aatcgggatg atgatggcga
    10201 tagccattgc cagcgcaaat acctgccagt agctcggttg aatcgagaga attcccggca
    10261 ggccccctac gccgatacca ttcgccataa cgccgttcag gccgcatagc aatcctgcca
    10321 gaccagaacc aatcatcgcg cacagcatcg ggaagcgata tttcaggttg atgccgtaca
    10381 ttgcaggctc agtgacccca agccaggcgg agatagcggc aggcacggag atctcgcgtt
    10441 cattgtgctt gcggctggaa atgataatgc ctatcacggc ggagccctga gcgatattcg
    10501 acagcgcaat cagcggccac actggcgtgc cacccatgct ttgaatcatc tgcaaatcaa
    10561 tagcaagggt ggtctggtgt acgccggtga tcaccagcgg cgcgtacagg aagccaaaca
    10621 atgccgcacc aatcggagca aagctgccgg tcatcaggtg acgtaccgca aaggcaacgc
    10681 catcgccaat catgcgacca aacggaccaa tcagcgcatg ggcgaggaac accgcgagga
    10741 tcagcgaaca gacgggcacc accaccagat agaggtaatc cggtacgatg cgtttaaggc
    10801 gagtttcaat aacgcccagc gccagcccgg ctaacagtgc cgggatcacc tgcgcctgat
    10861 agcccacttt ggcgatgctg aacatgccaa agtcccacac ttccggcagc tgctgcccga
    10921 gcagataagc gttcatcagc tggggggaaa ccagtgtcac gccaagcacg ataccaagga
    10981 tcggcgtgcc gcccattttt ttcaccgctg accagcaaat cccgaccggc aggtagaaga
    11041 aaatcgcttc accgatcaac cacagaaaat cgtagatcgt ttgcagggaa gggtacattt
    11101 gcgccagcgt ctgaccgttg ctcatgggca aatcgccgat cacattgcga aaaccgagga
    11161 tcaaaccgcc gctaatcaac gcgggcagca acgggaagaa gatctccgcg aaatgagaga
    11221 tcaactgctc atgccatttc atattttgcc gggcggcttt ttttacctgc tctttatcaa
    11281 cctgcgcctg tccggttgac gctattagtg cttgatagta atcacccacg ttggtgccaa
    11341 tcaccacctg aaattgcccg gcattggtga aacagccttt caccatgggg agttgctcaa
    11401 tttctttcgg tctggcattg gccggttggt tgaggacaaa gcgcaggcga gtaatacagt
    11461 ggctcaccgt cgcaatattg ccgcgcccgc cgaccagttc aatcaaccga tcgatatccg
    11521 tttggtttat tttgctcatc ataaagcccc atggcagatg acatttttgg ttggctgcag
    11581 aatatattgc gcggaaaaat ttaaaaacgg gaacgttccc gaaacgcagc gaagatcaca
    11641 atttatcgtt caggaaacga tcaggacagg gtggcgggga tgatgatttg ttgcggttcg
    11701 ctgcgcccgg ttacctgtgc gatcaattgg caggccgcct ggcgtccagc ttcggcgtag
    11761 ccaggatcta cggtgacgat ctccggatga aggaatttca ttaacggcgt attacctacg
    11821 ctcgccagtt gcaaggtgtc gatgcgttgc tcttgcaggt atttacttgc gccaagagcc
    11881 agcgtgtcgg ttgcgcacag taacgcggta gtttcaggcg taatcacttt tgctacgttt
    11941 tcatagcctt gcttcatagc aagccctggc agagcggcaa cgggatgcag tttatgcgct
    12001 ttgcagaacg ccaggtaggc ttcgtgacgt cgcttcccgg tcgtcacgtc actatgcggc
    12061 acgccgagat aactgatatt gcgatgcccc tggtcataga gccgttgcat cagaatcttg
    12121 attgcccctt cgtcgtcata acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga
    12181 accagcgatg actgccagtg ggctaacatt tcttcggtta tgccggtaaa accgaacagc
    12241 actacgccgt cgatattacg ccgtttcagc acccccaaat gttcggcaac taattgtggg
    12301 gaaaactgat tttccatcat gattggatcg taaccttgtt catagaacgc tggcagcatg
    12361 gtttgaacgg cgagattttc tgacaacgaa tccagacggg taacaatgat ggcgaccact
    12421 ttatcgcttt gcccacgcat agcgcgcgca gagcgggaag gggaaaatcc atgctgattc
    12481 atcactgctt caacacgctc gcgcgtgcgc tgactcacgc cgctttcgtt attcagcacc
    12541 cgggaaaccg tagatttccc cacgccgctt aagcgcgcga tgtctttgat ggtcagccga
    12601 ttttgcatcc tgttgtccca taacgtgttg tttaattatt tgagcctaac tttacccgtg
    12661 cattcagcaa tgggtaaagt ctggtttatc gttggtttag ttgccagcag gtattatatc
    12721 gccataaatg ctacgaatat tattggattc tccttattat ttgtggcgct tttttcactt
    12781 accggaggtt atatggaacc tgatcccacg cctctccctc gacggagatt aaaacttttc
    12841 cggtaagccc gtcttttcac ggcgttaccg gatgcgtaag gccgtgacgt tttaatgtcc
    12901 ctgctcagct ttattacctt caggtaaggc ttcgccacgc ctgaagacat ttctgtactg
    12961 tttcagacag tgcggaggga ctccttatgt ttaaagaaat ttttacccgg ttcattcgcc
    13021 atttaccttc ccgtctggtt catcgcgatc cattgcctgg cgcgcagcag acagtgaata
    13081 cggcggtccc gccgtcctta agtgcgcatt gcctgaaaat ggcggtgatg cccgaagaag
    13141 aattgtggaa aacgttcgac acccatccgg aaggattaaa tcaggcggaa gtggaatctg
    13201 cccgcgaaca acatggtgat aataaattac ccgcacaaca accgtcgccg tggtgggtac
    13261 atttatgggt ctgctatcgc aaccccttta atattttgct caccattctt ggtgctattt
    13321 cttacgccac ggaagattta tttgccgcag gtgttatcgc gctaatggtc gctatttcta
    13381 cgttgctgaa ctttattcag gaagctcgtt ccaccaaagc ggcagatgcc ctgaaagcga
    13441 tggtcagtaa taccgcgacg gtactgcgag taattaacga caaaggcgaa aatggctggc
    13501 tggagatccc gatagaccag ctggtgcccg gcgatattat aaaactggcg gcgggggata
    13561 tgatcccggc agatttacgt atcttgcagg cgcgggatct gttcgtcgct caggcgtcgt
    13621 taaccggtga gtctctgccc gtggaaaaag ccgctactac tcgccagccg gagcacagca
    13681 atccgctgga gtgcgacacc ctgtgtttta tgggcaccac cgtggtgagc ggcacggcac
    13741 aagcaatggt gattgctaca ggagccaaca cctggtttgg tcaactggcg gggcgtgtta
    13801 gtgagcagga aagcgagccg aatgcctttc agcaagggat cagccgcgtt agtatgctgc
    13861 tgattcgctt tatgctggtg atggctccgg tggtgttgct tatcaacggt tacaccaaag
    13921 gcgactggtg ggaagcggca ctgtttgcgc tttcggtcgc ggtaggctta acgccggaaa
    13981 tgttgccgat gattgttacc tcgacgctgg cgcgcggcgc agtaaagctg tcgaaacaga
    14041 aagtgatcgt caaacatctg gatgccattc agaactttgg cgcaatggat attctgtgca
    14101 ctgataaaac cggcaccctg acgcaggaca aaattgtgct ggagaatcat accgatatct
    14161 ccggtaaaac cagcgaacgc gtgctgcata gcgcgtggtt gaacagtcat taccagaccg
    14221 gacttaaaaa tctgcttgat accgcggtgc tcgaaggtac ggatgaagag tcagcgcgtt
    14281 cgctggccag ccgttggcaa aaaattgatg agattccgtt tgatttcgag cgtcgtcgga
    14341 tgtcggtggt agtggcagaa aataccgaac accatcagct ggtttgcaaa ggtgcattgc
    14401 aggaaatcct caatgtgtgt tcgcaggtgc gtcacaatgg cgagattgta ccgctcgatg
    14461 acaccatgct gcgtaagatt aagcgggtta ctgatacgct gaatcgtcag gggctgcgcg
    14521 tggttgcggt ggcgacgaaa tacctgccag cgcgtgaagg agattaccag cgggcggatg
    14581 aatccgacct gatcctcgaa ggatatattg ctttccttga tccgccaaaa gagacaaccg
    14641 ctccggcact gaaggcatta aaagcgagtg gaattaccgt aaaaatcctt actggcgaca
    14701 gtgagttagt tgcagcgaaa gtgtgccatg aagtggggct ggatgcggga gaggtggtca
    14761 ttggtagtga tattgaaacg ctatctgacg atgaactggc aaatctcgca cagcgcacca
    14821 cgctgtttgc ccgcctgacg ccgatgcata aagaacgcat tgtgacctta ctgaagcgcg
    14881 aagggcatgt ggttggcttt atgggcgatg gtattaatga tgcgcccgcc ttacgcgctg
    14941 cggatatcgg catttctgtg gacggcgcgg tagatattgc ccgtgaagcg gctgatatca
    15001 tcctgctgga aaaaagcctg atggtgctgg aagagggggt tattgaggga cgtcgcactt
    15061 tcgccaacat gctgaaatac atcaaaatga cggcgagttc taacttcggt aatgtgttca
    15121 gcgtgctggt ggcgagtgct ttcttgccct tcctgccgat gttgccgtta cacttgctta
    15181 ttcagaacct gctgtacgat gtgtcacagg tggcgatccc gtttgataac gtcgacgacg
    15241 agcaaattca aaagccgcag cgttggaatc cggcggatct ggggcgcttt atggtcttct
    15301 tcggaccgat cagctcgatt ttcgatattc tgacgttttg cttgatgtgg tgggtattcc
    15361 atgccaacac gccggaaacg caaacgctgt tccagtcggg ctggtttgtg gtgggattac
    15421 tgtcgcaaac gctgattgtg catatgatcc gtacccgccg tgtgccgttt attcagagct
    15481 gtgcatcgtg gccgttaatg atcatgaccg tgatcgtgat gattgtcggg atcgcattgc
    15541 cgttttcacc gctggccagt tatctgcagc tgcaggcgct gccgttaagc tatttcccgt
    15601 ggctggttgc gattctggca gggtatatga cgttaaccca gttggtgaaa gggttctata
    15661 gccgtcgtta cggctggcag taaagaataa actgggcatg gtggcccgga ttcagattgc
    15721 agacaaagtg agctttgttc atgccggatg cggcgtaaac gcccaatccg gcctacaaat
    15781 tcaataaatt gcaggaatca cgtaggcctg ataagcgtag cgcatcgggc gattttgctt
    15841 ttgtcatcag acttaatccg ggcatgatag cccggatttc catcaagatt agcgacgaac
    15901 agcgattgct tcgatctcaa tcttcacgtc tttcggcaga cgggcaactt caacgcaaga
    15961 acgtgccggg aaagtggcgt tgtgttcggt gaagaaggct tcgtaagtgg cgtttacggt
    16021 tgcgaagtcg ttcagatctt tcacgaacac agtggtttta acgatgtcgc ccactttcag
    16081 gccagcggct tcgacgattg cttttacgtt atccagcgac tgacgtgcct gtgcagcgac
    16141 gtctgccggt acttcaccag ttttcggatt taccgggatc tgaccggagg tgatgatcat
    16201 attgcccaga tcaacgccct gtacgtaagg accgatagct gccggtgcat tttccgtcgc
    16261 gatagttttg ctcatgattt ctcctttatt acagcggtaa aaagtctggc tcattatagg
    16321 gagccagact tttattacca accgcaatta attggccagc accacattat gggaaaactc
    16381 tttttcacag tatttgcatt taagcgcgat gtcattggcg cgttttcgca cggcaaagct
    16441 ggatgaaacc ggttcggcat ggctgataca gttgctgttc gggcagacca gcacattgtc
    16501 gatgcgctcc ggcagacttg ggcgcgattt acccaccact tcatagttgt cgatacggtt
    16561 aaccgtggct tgcggtgcat acaatgccag ttggtctact tgatcttcac tcaaaaaggt
    16621 attttcgatt ttgatcagat ctttgcggcc catctcgcca gaaggcaaat tcagaccaat
    16681 ggtgatgcgc tgatctgttt cggtcaactt gaacagactc aacagcttaa aaccgatctg
    16741 ggcggggata tggtcaatta ccgtgccgcg tttaatagct tcaacctgca atttattatc
    16801 gtgtgtcatc tctatttccc cttacagtac cagatcgcga ttcagaacca gtgccagtaa
    16861 cgcctggcga gcgaaaattc cgttgcctgc ttgctggaag taccaggcgt gaggcgtttt
    16921 atccacatcc gtcgcaatct catcaacacg cggcagcgga tgcagcactt tcatattggc
    16981 tttggcgttg tggagatcgc tggcgcgaag aacaaactgc gctttcacgt tggcgtactc
    17041 ggacgggtcc agacgctctt tttgcacgcg ggtcatgtac aggatgtcta cttccgccat
    17101 cacttcttca atagagctgt gcagactcca ggcgatccct ttttcatcga gcatatccag
    17161 aatgtattgc ggcattgcca gcgcatccgg cgcgataaag taaaaacggt tgccgtcgaa
    17221 cttcgctagc gcctgggtca gggagtgaac ggtgcggcca tatttcaggt caccaaccat
    17281 tgcgacgtgg agattgtcca gacgcccctg ggtttcctga atggtgaata agtccagcaa
    17341 ggtttgcgtc ggatgttggt tggagccatc accggcattc agtaccggta cattgccaga
    17401 aaattcggtg gccaggcgcg ccgcaccttc ctgcggatga cgcatcacta tcgcatcgac
    17461 gtaagtgctg ataaccgaaa tggtatcggc cagcgtttcg ccttttttac ccagtgatgt
    17521 attggcgctg tcggagaagc ccaccacgct ggcacccagg cggtgcatgg aagtttcgaa
    17581 agagaggcgg gtacgggtag aggcttcgaa gaaacagctg gcaatgactt tgtgcttcaa
    17641 cagctctggt tgcgggtttg ctttcagttt cgccgctgtc gccagcacca gattaaggtc
    17701 atcgcgacta aggtcgttta tggaaatgat atgtttctga tatagcggat tagccatctt
    17761 ttatctcctg acgcctaggc aaaaaaaagc ccctcgattg aggggctggg aatgggtgat
    17821 caacgggaag aaaaacggca ggccagcgtc ttttttcaga cgcggtaaga caaaatgtcg
    17881 aacacactga accatacatc ctcccggcaa attgtccggc attatactca tagccagaag
    17941 cggtgcaagc atttgatgca atattttgtc agcgcaaacg gtttatttga attaaaagtc
    18001 aaaatatatg catttttatt ttctatgatt ctggagggga agtgtcgttc ggtatcaata
    18061 attgcagaca tttcctgctt ttttcttttt cattttcacg caatcagaaa tgcattttat
    18121 tccattcatc cgacttatag ggcggagggt gaaaacgaac ggctaacact attgcttata
    18181 gctcagggat acgcgctatc actttaattt caaaatcaaa gcctgccagc caggtaacac
    18241 ccaccgccgt ccagtttgga taaggtgggg cgctaaatat ttcatttttc accgtcatga
    18301 tgtcttcaaa ttgtttttcc ggatcggtat gaaagctcgt aacatcaatg atatcgtcaa
    18361 aagtgcatcc cgcagctgcc agggtcgcat gcaaattatc aaaggccagt ctgacttgtt
    18421 gctgaaaatc gggttctggt gttccgtcct ctcgacttcc tacttgcccg gaaacaaaca
    18481 gcaaatcgcc ggaacgaata gccgcagaat aacgatgctc agcatacagt gaatgtcggc
    18541 cagcagggaa aacagcggtt ctttctacca tttggttatc ctcaagattt acgacatgaa
    18601 cagaagattt atatttaccg ggagccgctt ttagggggac gacgttagta aacaaaaccc
    18661 agacatcatg gataatggct gggcttaatt gagcgtagtc ggttatgcgc caaacgcgcc
    18721 atcaatggta tgcatcgcgc cggtaacaaa actggcttct ggccctgcta accatgcgac
    18781 cataccagcg acctcttccg gttgcccatg tcttttgatg gccataaaac tatgcaacat
    18841 atcgcgtatt ggcccgttgg cgggattaac gtcggtatca attggtcctg gctggacgac
    18901 gttgatggtg atccctcgcg gtccaaaatc acgggccagc ccgcgcgcca tgccttgcag
    18961 ggcagattta ctggcggcat aagcagccat gcctgcaaca ggcatacgat cgccattcac
    19021 ggagccgatg attaagatgc gcccgccttc gggcatctgc cgggcggctt caacagaggc
    19081 atgataagga gcatgaatat tgattttgaa aaggcgatca atatcgtcgg catttaattc
    19141 cagggcatcg ccaaagacgc caatacctgc atttaccacc aggatatcca atgtgccgct
    19201 cttacgaacg acatcaataa cagcgtctct gtcagcacta tctgtgaata ctgctgtcgc
    19261 tccagtctct tgtgccagac gttcagcggc atctttcgac cccgcatagg tgaatcgtac
    19321 attggcccca tcggtgacga aacgacgtac gatagcggca ccgattccac gactgccacc
    19381 gaggatgaga actgtcttac cggtaaaagc gcccataagg actccttgat ttattatgta
    19441 acatgcatta caaaactgtt ttaactttct gtcaacaagt tttgtaatgg tcactaaaaa
    19501 acaatctcgc gttccaggtc gtcccagacg tttcgctcct gagcaggcag tctctgcggc
    19561 aaaagtgctt tttcaccaaa aaggtttcga tgcagtaagt gtctctgaag ttactgatta
    19621 tcttggtatt aaccccccga gcctctacgc ggcttttggc agtaaggctg ggttatttag
    19681 ccgtgtactc aatgaatatg tcggtacgga agctattccg cttgccgata ttcttcgtga
    19741 tgatcgtccg gtaggcgagt gcctggttga ggtattaaaa gaagcggcgc gcagatatag
    19801 ccaaaacggc ggctgcgctg gctgtatggt tcttgaaggt attcatagtc atgatccaca
    19861 agcgcgtgat attgccattc aatattatca cgccgcagaa acgaccattt atgactatat
    19921 cgccaggcgg catccacaaa gcgcacaatg tgtgactgat tttatgagta ccgtgatgtc
    19981 agggctttct gcgaaggcac gggaggggca ctccatcgaa caactctgtg caacagctgc
    20041 cctggcgggg gaagcgataa aaactcttct caaggagtga tgctggcctt gatccgaaag
    20101 gcgggaacgc gcctgccgat aagttgtgat aagacaataa ttcacgcgtt aaggctggcg
    20161 gaattgatca tcttttcgta taacgataga aatgaaacat tgttttaatt aaggagtgga
    20221 aaagatgatc atcggaaata ttcataatct tcagccgtgg ctaccccagg agttacgcca
    20281 ggcgattgag catatcaaag cacacgttac ggcagaaacg ccaaagggca agcacgatat
    20341 cgaaggcaat cgtctgtttt atcttatctc ggaagatatg accgagccgt acgaagctcg
    20401 ccgtgcggag taccatgccc gctatcttga cattcagatt gtgttaaaag gtcaggaagg
    20461 catgaccttc agcacgcaac ctgcaggcgc gccggatacc gactggttag ctgataaaga
    20521 catcgcattt ttgccggaag gcgttgatga gaaaacagtt atcctaaatg aaggtgattt
    20581 tgttgtgttt tatccggggg aagtgcataa accgctgtgc gcagtgggcg cgccagccca
    20641 ggttcgcaaa gcagtagtga agatgctgat ggcgtgatga cttttcgccg taaataactc
    20701 caggtttacg gcgagtttgt gaaaagagcg ttttttgata tttttttgtg agtaaaattt
    20761 gtaatgctta gacgttctta ttaactcaag gagttcgtca tgagcaaaat atcagatttg
    20821 aattattctc aacacattac attagccgac aattttaaac aaaaaaatga agccttagat
    20881 acctggtatg tgggaatgaa tgattttgcc cgaattgccg gggggcagaa tagcagaagc
    20941 aatattcttt ctcctagagc atttttggag tttttggcaa agatatttac cctgggttat
    21001 gtggatttta gcaaacgctc caacgaagcg ggtagaaata tgatggctca tattgagttc
    21061 tcatcttatt ctaaagtact gatggcaatg aaaaaatgaa gttttacatg aataatcctg
    21121 aaggggagcg agcggattta tctaaggtga agacagaaat tacacttgcc tcagcttcta
    21181 ctaaagggat tcgtgaagga catacagtca ttatatttaa acaatctgat ggttcgacta
    21241 atcgttatga agggaagtct tttgaaagaa aagatgatag ttcattacac ctgattacta
    21301 acaaggttct ggcgtgttac caacgcgaag ctaacaagaa aatagcgcgt ctattaaata
    21361 atcatcagaa gttaaataat ctacagaagt taaataatat acaggagtta aatgattcgc
    21421 aggagttaaa taattcacag aagttaaata attcgcagga gttaaatgat tctcaggagt
    21481 taaataactc gcaggtgagt tgtaaaggtt cagttgattc tacgattacg gatttattag
    21541 aaaaagcatt gaataaagcc ttattagcaa taaggaacga acatctgcta ttaatgcctc
    21601 atgtatgtga tgaatagatt tcatacttac tgggcgaaaa tggtatactt gaagaaatag
    21661 ataagctcta cgaattaaat gatcacggaa ttgataatga caaagaaggt aacaatgaaa
    21721 ttaatgacat catgattaac ctgtctcata ttcttattga ttccttagat gatgcaaagg
    21781 ttaaccttac accggtcatc cattcgatgt tggtgacttt tttagaattg ccatataata
    21841 atgatgtaaa aatactggag tggtgtttta ataaaagcat gcaatatttt gatgattctg
    21901 caaagataga gcatgcatgc tccgtaataa atcatattaa ttttctctgc gatcagtcta
    21961 aagtagctga gacattattt ttcaatctcg ataaagaacc ctataaaaat agccctgaat
    22021 tacaggagtt gatttggaaa aagttggttg tatatgtcaa tgattttaac ttaagcaatc
    22081 gagaaaaaac atatttaata caaaggatat ttaataatgt tgagtcgcta tttaataaag
    22141 tacctgtcag cattttagtt aatgatattc ttctgaatga tttttttatg aaaaacactg
    22201 agatgattaa ctggtacttt cctcggttac ttaagagtta tgagggcgaa aaaaattatt
    22261 ttgataattt aaaatatgat ataaatgatg agaaatctaa taaagaaatt ttgaagaatc
    22321 aaccagataa tgttatcaaa gaaaaactga ataatgaatt caaacttaga tttagaatga
    22381 tgcaaactat ctttaaatcg aaggttaatg tatcgccata tattgaccaa cagcgtttaa
    22441 atacactaaa tcctccggaa aatttacgta tggcaataga aaaatttggc tggaagaaaa
    22501 aaactatcac tgcataaaat aatttgatgc cgggatgttt ttgtatttcc cggcatcttt
    22561 atagagatat taattattta atttactgag cgtcgcgacc atcaccgctt tgatggtatg
    22621 catgcggttt tccgcctgat caaatacaat gctggcggca gattcgaaga cctcatcagt
    22681 cacttccatt ccgccatgta ggccaaactc ttccgccatt tttttgccaa gcgtcgtttg
    22741 gtcgtcatga aacgcgggca ggcagtggag gaatttgacc tccgggttac cggtcaactg
    22801 catcatcttg ctgttcacct gataatcacg cagtaatgca atgcgttcag cccatttttc
    22861 ttttgcttca cccatcgaca cccatacatc ggtatagata aagtcagcac cttcaactcc
    22921 tttagctaca tcttccgtca gcgtaatatt cccgccattt tgttgtgcca gggcgcggca
    22981 ttccgtaacc agcgcagctt ctggccagca tgcctgtggc gcgaccagac gcaaatccag
    23041 accggtaagc gccgcagctt cgagcatcga attgcccatg ttgttacgtg cgtcacctgc
    23101 atagaccagc gtcatttcgt tgaatgcttt accgggtaaa tgctcctgca tggtgaggag
    23161 atccgccagc aactgcgtgg ggtgaaactc attggtcagg ccgttccata ccggcacgcc
    23221 agcgtattcc gccagtgttt cgacaatctc ctgaccatag ccgcgatact gaataccgtc
    23281 atacatgcga ccaagcacgc gggcagtgtc tttaatcgac tctttatgac caatctgact
    23341 accgcttggg ccaagataag taacgcgagc accctggtca tatgcggcaa cttcgaaaga
    23401 gcatcgggta cgagtcgagt ctttttcgaa gatgagcgcg atgtttttac cagtgagttt
    23461 ggcttcttct ttaccgcttt tcttatcagc tttcagcttc gcggctaact gcagcaggct
    23521 gttgagttca gctggcgtga aatcgagtaa tttcaggaaa tgcttatgat aaaacccgga
    23581 catagatccc tcctgtggct aacgcctcaa tgaattaaaa ttcaatttat atgtatgatt
    23641 attcatttgc aagtctaaag cataaatctt tgtcacaaag gtggaggcaa tgtcagtggt
    23701 gtgtgacaat aagagtatcg gcaggacatt aagaggaatg agccatggca aacccggaac
    23761 aactggaaga acagcgtgaa gaaacacgtt tgattattga agaattactg gaagatggca
    23821 gcgatccgga cgcgctgtac accatcgaac accatctttc cgcagacgat ctggaaactc
    23881 tggaaaaagc ggcagttgaa gcgtttaaac tcggttacga agtgaccgat ccagaagagc
    23941 tggaagttga agatggtgat atcgtgattt gctgcgacat cctcagcgag tgcgcgttga
    24001 atgccgatct gatcgatgcc caggttgaac aactgatgac gctggcggag aaatttgacg
    24061 ttgagtacga cggttggggc acttactttg aagatcctaa cggcgaagat ggcgacgatg
    24121 aagattttgt cgatgaagac gatgacgggg ttcgccacta attaattgat taaaatgccc
    24181 gatagcacat actagcgggc atttttttag agttcgcgta gcatccgcac ttcacaatcg
    24241 acatggcccg tgcagccaag cgcatagtcg atatgctcaa agcccaaatg ctcataaagc
    24301 gcaatggctt cctttaaaaa agcggtcgtt tccagatagc agcgtttgaa gcccatctct
    24361 cgtgcctgct ccatcgccat taaggccagt tttttcgcta gccctttacc gcgaatagcg
    24421 ggaagaaagt acattttctg cagttcgcaa atgtccgctt cactacctgt taatggcgca
    24481 atcccgccgc cgccgaccac ttcgccattg tattcaacca cccaataagc atggccaggc
    24541 tggctgtaca cttgatacag ttcgtcgaga ttcggatcgg caacggtgta gcctttatcg
    24601 gcggtaagac catactctgc gtaaacctga cggatgacgc tggcgatagc aggattatcc
    24661 tgcagcgtca ggcgacgcat tacaggtgat tgcggcgcaa tgttattcat agtttaaccc
    24721 aataaaaata aagtaattat acatttgttt aataccactc tcttcttacg tgcgcaagcg
    24781 tcttataaag caataaagtt tttgccagcc tattactgat aagagtattg taacgtggca
    24841 gaacatttta ctgtctgtat ctataaatta tatcaatgga ttttttaatt caggggattt
    24901 ttatggctca agttattaat gaaatggatg ttccgtccca ttcttttgtt tttcatggta
    24961 caggtgagag atattttctt atttgtgtgg tgaatgtgtt gttaacgatt ataacgctag
    25021 gtatctattt accatgggca ttaatgaaat gtaagcgtta tctttatgct aatatggaag
    25081 ttaacggaca acgattttct tatggaatta ccggtgggaa tgtttttgtt agttgtcttg
    25141 tttttgtttt tttctatttc gcaatcttaa tgacagtgtc agcagatatg ccgcttgttg
    25201 gctgtgtttt gactttgtta ctgttggttt tgcttatatt tatggcagca aaaggactgc
    25261 gttatcaggc cttaatgacc agtctcaacg gcgtaagatt tagttttaat tgctctttga
    25321 aagggttctg gtgggtaacc tttttcttgc cgattttaat ggccattggg atggggactg
    25381 ttttctttat ctcgacaaag atgctacatg ccaatagttc aagtagtgtt attatatctg
    25441 tggttctgat ggcaatagtt ggtattgttt ccattggtat ttttaatggt actttatata
    25501 gcctggtaat gagttttctc tggagcaata ccagtttcgg tatacatcgt ttcaaggtga
    25561 aattagatac tgcgtattgt ataaaatatg ccatcctcgc atttttagct ttattacctt
    25621 ttctcgctgt tgctggttat attatcttcg atcaaatatt aaatgagtat gatagttctg
    25681 ggtatgcaaa tgatgatatt gagaatttac agcaatttat ggaaatgaaa cgtaaaatga
    25741 taatcgcgca gttaatttat tattttggga ttgctgttag caccagttat ttaacggtgt
    25801 cgttgcgaaa tcattttatg agcaacctgt cattgaatga tgggcgtatt cgttttcgct
    25861 caactttaac gtaccacggt atgctttatc gcatgtgtgc gttggtggtg atatccggga
    25921 ttacgggtgg tctggcttac ccactgctga aaatatggat gattgactgg caggcaaaaa
    25981 atacgtattt gctgggcgat ttggatgacc ttcctttaat caataaagaa gaacaaccag
    26041 ataaaggctt cttagccagt atttctcggg gagttatgcc ttctttacca tttctgtaag
    26101 agataaaaaa ggccggagca tgctccggcc ttcgttttca tcactgtgtt gattacagtg
    26161 cggcgataac agcctgctgt tcaatcagct tggctttcgc ttctgcataa ccttccagct
    26221 tctcacgctc tttcgcgata accgcttccg gtgcgcgggc gacaaagcct tcatttgcca
    26281 gcttgttctc gatgcggctg atttcacctt cgatcttcgc cacttctttc gccaggcgcg
    26341 ccagctcatc ttctttgttg atgaggccag ccatcgggat cagcagctct gcgccgtcga
    26401 tgatcttggt aacggaaacc ggacctttgt catcggcagg cagcacggtg atgctttcca
    26461 gacgcgccag cgtttgcagg aagccacggt tttcatttac gcgacgttct gcatccgcgc
    26521 tgcaaccacg cagcagcagc tccagcggtt tgcccggcgc gatgttcatt tctgcacgga
    26581 tgttacgtac agcaacgatc gcctgtttca gccactcggt gtcggccagt gcggcttcat
    26641 caacctgaga tgcatcatac tgcgggaacg gctgcagcat gatggtgtcg gcagtgatac
    26701 cgcaaagtac tttcacacgc tgccagatgg tttcggtgat gaacggaatg atcggatgcg
    26761 cgaggcgcag cagaccttcc agtacagtca ccagcgtatg gcgagtaccg cgcagttcgg
    26821 cttctgttcc accgttcatt accggcttgg tcagctccag ataccagtcg cagaactggt
    26881 tccaggtgaa ctcatacaaa atgcctgcgg cgatatcgaa gcggaagctg tccagcgctt
    26941 cgcggtacgc tttgatagtc tggttgaact ccgccagaat ccagcggtcc gccagcgata
    27001 gcgtcatttc gccgccgttg aagccgcaat cctgaccttc tgtgttcatc agcacaaagc
    27061 ggctggcgtt ccacagcttg ttacagaagt tacggtagcc ttccagacgc ttcatatccc
    27121 agttgatgtc acgaccggta gacgccagcg ccgccagggt gaagcgcagg gcatcggtgc
    27181 cgtgcggctc aatgccgttc gggaactgtt tctcggtgcg cttacggatt ttgtccgcca
    27241 gctgtggctg catcatattg ccggtacgtt tttccagcag ttctggcagc gaaataccgt
    27301 caaccatatc cagcgggtcg ataacgttac ccttggattt ggacatcttc tgaccttcgt
    27361 cgtcacgaat cagaccggtc atgtaaacag tgtggaacgg cacctgcggt ttgccatttt
    27421 catctttgat gaagtgcatg gtcatcatga tcatgcgggc aatccagaag aagatgatgt
    27481 cgaaaccaga taccatcacg ctggttgggt ggaactgacg cagggcgtcg gtattttccg
    27541 gccagccaag ggtagagaag gtccacagtg cagaagagaa ccaggtgtcg agaacgtctt
    27601 cgtcctgacg cagggcaacg ttggcaccga ggttattttc cttgcgcact tcttcttcgt
    27661 tgcggccaac ataaacatta cccgcttcgt cataccatgc cgggatacgg tgaccccacc
    27721 acagctgacg agagatacac cagtcctgaa tatcgcgcat ccaggagaag tacatgtttt
    27781 cgtactgctt cggtacgaac tgaatgtcgc cgttctcaac cgcttcaact gccggtttcg
    27841 ccagtacatc ggcacgcacg taccactggt cggtcagcat cggttcgata accacgccgc
    27901 cacggtcgcc gtaaggaacg gtcaggtcgt gcggtttaat ttcttccagc aggccgagtg
    27961 catcaaccgc agcaacgact gctttacgtg cagcaaaacg ctcgagtttc tggaactctg
    28021 ccgggatctc gctggaataa acgtcagatt cgttaccttt ggtatcgaac acctgggcgc
    28081 tttcacggat atcaccgtca aaggtcagga tgttgatcat cggcagggcg tgacgtttac
    28141 cgacttcata gtcgttaaag tcgtgcgccg gggtgatctt cacgcagccg gtgccttttt
    28201 ccatgtcggc gtgttcgtcg ccaacgatcg gaatacgacg gttaaccagc ggcagaatga
    28261 catatttgcc aatcagatct ttgtaacgcg gatcttccgg gttaacagct acaccggtat
    28321 cgcccagcag ggtttccggg cgagtggtcg caaccaccag ataatcttta ccgtctgcgg
    28381 ttttcgcgcc gtcagccagc ggatagcgga tgtgccacat cgaacctttc gattcgtggt
    28441 tttccacttc caggtcagaa atagcggtac gcagtttcgg gtcccagttt accaggcgct
    28501 tgccacggta aatcaggtct tctttataca gacgaacgaa aacttctttc accgcattgg
    28561 acaggccttc gtccatggtg aagcgttcac gctcccagtc gacggagttg ccgagacggc
    28621 gcatctgacg ggtaatggtg ccgccagatt ccgctttcca ttcccagatt ttgtcgatga
    28681 aagcttcgcg gccgtagtcg tgacgggttt taccttcttc tgcggcaatc ttgcgctcaa
    28741 cgaccatctg ggtagcgatc ccggcgtggt cagtaccgac ctgccacagg gtgtttttgc
    28801 cctgcatgcg ctgatagcgg atcatggtat ccatgatggt ttgctggaag gcgtgaccca
    28861 tatgcaaact gccggtgacg ttcggcggcg ggatcatgat gcagaaactt tcctggcttt
    28921 catcgccatt aggcttaaag tagccctgct tttcccagtg ctcgtaaagc ggctgttcga
    28981 tatcttgtgg gttgtatgtc ttttccatta tttccaggtt gccgtattca ggttgaaacc
    29041 agccacgcgg taggctttat agcgttcgcg cgccagttgt ttcagagaat cttcataagg
    29101 aacgaagtct accacttctg tgaaagcggt ggcaaaatct gcaaagcttg ttcgcagact
    29161 aatcaatata tcgcgcgggc tgctgctgcg tttttgcggc caggcgatct ccaccggcgc
    29221 accgccgcgc ggtccttctc ccgctaaatt atgcggaaca aagctttctg ccggacgcgc
    29281 ccacagggct tcatccagcc ggtaagcctg cttttcatct tcacaggcga tgagtacgcg
    29341 cttaccgctg cgccaacgtt ctgcggcaat ttcacacacc agttgctcaa cggcgcttaa
    29401 gccatcgacg gtggtgtcat tgtccagaag gtagaacgtt gcgtttttca tatatggggc
    29461 ttcttgtggt ggatttaaat attgctgcat tcctcaccct gaaagatgag caatgaattt
    29521 ctattgcgct cgatcggttc cctctccctt agggagaggg ttagggtgag ggtaaaacgc
    29581 aaacttactc ttcgccgtta aaccgagcgc ggtttaacag gaactgtgcc agcaacgcta
    29641 ccggacgacc ggttgcgcct tttgctttac cagaacgcca ggcggttcct gcaatatcca
    29701 ggtgcgccca gttatactta cgggtaaagc gtgacaggaa gcaacctgcg gtaatcgccc
    29761 caccaggacg accgccaatg ttcgccatat cggcaaaatt ggactccagc tgttcctgat
    29821 actcgtcacc cagcggtaag cgccatgcgc ggtcaccgga ttgttcagac gcggcaatca
    29881 gttcatgggc cagcggatta tggttcgcca tcagaccagt aatatgatga cccagcgcga
    29941 tcacgcaggc accggtcagc gtcgccacgt caatcactgc ttccggctca aaacgttcaa
    30001 cgtaagttaa cacgtcgcac agtaccaggc ggccttcggc atcggtgttc agcacttcaa
    30061 cggtttgacc ggacatggtg gttaacacat cgcccggacg ataggcacgc ccgccaggca
    30121 tgttttcaca gcctgccagc acgccaataa cgttaatcgg cagttgcagc tccgcgacca
    30181 tacgcatcac gccgtaaacc gccgccgcgc cgcacatatc gtacttcatc tcatccatgc
    30241 cttctgaagg cttgatggag ataccgccgg agtcgaaggt taaaccttta cccaccagca
    30301 cgattgggcg tgcatcttcc gacgcgttgc ctttgtactc aatcaccgac atcagcgatt
    30361 cgttttgtga accctgaccg accgccagat aagagtgcat tcccagctct ttcatctgct
    30421 gttcgccgat aacgcgggtg atgacattct tgctgtagct gtcagccagc tggcgcgcct
    30481 gagaggcgag ataagcggcg ttacagatat tcggcggcat attaccgaga tctttcgctg
    30541 cttttatccc ggcggcaatc gccagaccgt gctggatcgc gcgctcaccg ctggtcagtt
    30601 cacggcgggt cggcacgttg aacaccattt tacgcagcgg acgacgcggt tcgctcttat
    30661 tcgttttcaa ctgatcgaaa ctgtagagcg tctcttttgc tgtctcgaca gcctgacgca
    30721 ctttccagta gttgttacgg cctttaacgt gcagttcagt cagaaagcag accgcttcca
    30781 tcgagccagt atcattcagc gtattaatgg ttttctgaat aacctgcttg tactgacgct
    30841 catccagctc acgttctttg ccgcaaccaa taaggagaat tcgctcggaa agtacattcg
    30901 gaacatggtg cagcaacaat gtctgccccg gttttccttc cagttcgccc cggcgtagca
    30961 gggcgctgat gtacccatcg ctgattttat cgagctgttc tgcaatcgga gaaaggcgac
    31021 gtggttcgaa gacgcccacg acgatgcagg cactccgctg tttctccggg ctaccgcttt
    31081 ttacactaaa ctccatgcac tacgctcctg aatcttaaag acaacggcgg tggctacaga
    31141 tagaattgca agctttcgta actcatgtcc gctgttgcga tgacttcgtg ttaatcttaa
    31201 cgttattacg gcattggcac gtcagaacaa aatctgagag gtgaatccgt tgagtataat
    31261 tatcttagcg acgatttcga cgactcaaga gaataaatga cgtttaagcc atgaaacaag
    31321 ctaaaatcct gcaaaagacg agtttttacg ggcgtattta aagtgataat cataagatat
    31381 ctggtgcggg agacgctcaa aagccagctg gcgatactct tcatcttgct tttgatcttc
    31441 ttctgtcaaa agttagtgag gatcctcggc gcagcggttg acggcgatat tccggcgaat
    31501 ctggtgctct cccttctcgg gttgggcgtg ccggaaatgg cgcagcttat cctgccatta
    31561 agcctgttcc tcgggctgct gatgacgctg ggcaaactgt ataccgaaag tgaaattacg
    31621 gtaatgcatg cctgcggctt gagcaaagcg gttctggtga aagcggcaat gatccttgcg
    31681 gtattcacgg caatcgtcgc ggcggttaac gtgatgtggg cgggaccgtg gtcatcgcgt
    31741 catcaggatg aagtgttagc agaagcgaaa gcgaaccctg gcatggcggc gctggcgcaa
    31801 gggcaattcc agcaagcgac taatggcagc tcggtgctgt tcatcgaaag cgttgacggc
    31861 agcgatttca aagatgtgtt cctcgcacaa attcgaccaa aaggtaatgc acgtccttct
    31921 gtggtggtgg ccgattccgg acatttaacc cagctgcgcg acggctccca ggtcgtcact
    31981 ctcaaccagg gaacgcgctt cgaaggcact gcactgttac gtgatttccg cattacggat
    32041 ttccaggatt atcaggcgat cattggtcac caggcggtgg cgctcgaccc gaacgatacc
    32101 gaccagatgg acatgcgcac attgtggaac actgacaccg atcgtgctcg cgcagaactg
    32161 aactggcgta tcacgttggt attcaccgtg tttatgatgg cacttatggt tgtaccgttg
    32221 agcgtggtta acccgcgtca gggacgcgta ctgtcgatgc tgccagccat gctgctgtat
    32281 ctacttttct tcctgatcca gacctccctg aaatcgaacg gcggtaaagg taagctggac
    32341 ccgacactgt ggatgtggac cgttaacctg atttatctgg ctttggcgat tgttctcaac
    32401 ctttgggaca ccgtgccggt ccgccgcctg cgcgccagtt tttcgcgtaa aggagcggtg
    32461 tgatgcaacc ttttggcgta cttgaccgct atatcggtaa aactattttc accaccatca
    32521 tgatgacgct gttcatgctg gtgtcgctgt cgggcattat caaatttgtc gatcagctga
    32581 aaaaagccgg gcaggggagt tacgacgcgt taggcgcagg aatgtatacc ttgctgagcg
    32641 tgccgaaaga tgtgcagatc ttcttcccga tggcggctct tcttggggcg ttgcttggtc
    32701 ttgggatgct ggcgcagcgc agcgaactgg tggtgatgca ggcttctggt tttacccgta
    32761 tgcaggtagc gctgtcggtg atgaaaaccg ccattccact ggtcttgctg acgatggcga
    32821 ttggcgaatg ggtcgcgccg cagggcgaac agatggcgcg taactaccgt gcgcaggcga
    32881 tgtacggcgg ctcgttgctc tctacccagc aaggtttatg ggcgaaagat ggcaacaact
    32941 tcgtctacat tgagcgggtt aaaggtgacg aggagttagg tggcatcagt atttatgcct
    33001 ttaacgagaa tcgtcgtctg caatccgtac gttatgccgc taccgcgaag tttgacccgg
    33061 aacataaagt ctggcgtctg tcgcaggttg atgaatctga tctgaccaat ccgaaacaga
    33121 ttaccggttc gcagacggtg agcggcacct ggaaaaccga cctcacgcca gacaaactgg
    33181 gcgtggtggc gctggacccg gatgcactct ccatcagtgg tttgcacaac tacgtgaagt
    33241 atctgaagtc gagcggtcag gatgccggac gttatcagct caacatgtgg agcaaaatct
    33301 tccagccgct atccgtggcg gtgatgatgc tgatggcgct gtcgtttatc tttggcccac
    33361 tgcgtagcgt accgatgggc gtgcgtgtgg tcacaggtat cagcttcggt tttgtcttct
    33421 acgtactgga ccagatcttc ggcccgctga cgttggttta cggcatcccg ccgatcatcg
    33481 gcgcactgtt gccaagtgcc agcttcttct taatcagcct gtggctgtta atgagaaaat
    33541 cgtaatctaa caagggagcg aaagctccct ttattattgt tgacaaagtc aaatacgagt
    33601 tcgaacggtc ccctcgcccc tttggggaga gggttagggt gaggggcaca gatcacgtct
    33661 gacttatctt tttctccccc ccagcaaact ccccaacatc ccgcgtacaa tctgattcgt
    33721 cacctggcga gcggcgcttt tcgccattgt ttgcaccaca ccatctttct tcccgccgcg
    33781 tggtccggta gtgccaaaca aaatatcctt caatccacca agaataccgt catccaccgc
    33841 cacctctttc cctttcacgg ggggattatt ttgctgctcg gtactggcct gaaagccttt
    33901 ttgcagcatc tcataggcgg actcgcggtc cacctcatct tcatatttgc catacaccgg
    33961 ggagtgatta atcaggccat tacgctcatc ttccgtcacc ggccccattc gcgaacaagg
    34021 tgcgatcacc atcgcccgtt ccaccacaga aggacttcct tttgcatcga gaaacgagat
    34081 taacgcctcg ccggtcccca gttcctggat tgccttttcg gtatcaaatg ccggattggc
    34141 ccgcatggtt tgcgccgctg tcttcactgc tttctgatct tttggcgtaa aagcccgcaa
    34201 agcgtgctga acacgattac cgagctggcc gagcacatta tccggaatat cagacgggtt
    34261 ttgcgaaacg aaccagacgc ccacgccttt tgagcgaata agccttatca cctgctcaat
    34321 cttatccagc agtacctgcg gtgcatcgtt aaacagcaga tgtgcctcgt cgaagaaaaa
    34381 caccagtttt ggtttctcca gatcgcccgc ttccggcaat tgttcataca actctgaaag
    34441 catccacagc aggctggcgg cgtacagttt cggcatctga taaagtttct cggcgctgag
    34501 gatattgata acgcctttac cgttggcatc ggtgcgcatc cagtctttga tatccagcat
    34561 cggctcacca aagaagtgtg ctgcgccttg ctgttccagc gacagtaatc cgcgctggat
    34621 ggcaccaacc gatgcgctac tgatatttcc gtactgattc tggaaggatt tggcgttatc
    34681 gccgatgtac tgggtaatcg cccgcaaatc tttaaagtcg agcagcaaca atccctgatc
    34741 gtccgcaatg cggaagataa tattcagcac gccagattgc acatcgttga gattcaacag
    34801 tcgcgccagc aacagcggcc ccagatccga aaccgtcgcc cgcaccgtgt ggcctttctc
    34861 gccaaagata tcccacacca ccaccggatt ggcatgcggt tgccagtcat tgacgccgat
    34921 atttttaagc cttgtgagca gtttttccga cgccgttcct gcctgcgcga taccggtcag
    34981 atcgcctttc acatcagcca taaacaccgg cacgccgatt tccgacaatg actctgccag
    35041 tttttgcagc gtaacggttt tacccgtccc cgttgcgcca gtaatcagcc cgtgacggtt
    35101 agccattccc ggcagtaaaa acagttctgt gtccggcgtg cgggcaatta acaggggttc
    35161 gctcatgaga tttcctccag tttcctgcct ggagtatagg caacggctgt gcactcttca
    35221 ccgctaaatt cagcagattg cgagacgcct tccaaagaga tttaagtgcc gggcgacagc
    35281 acccggcaaa gggatcagaa gtcggctttc aggactactc ggtagcgcgc tttaccatcg
    35341 cgtacatgct gaatggcgtc gttaattttc gacatcggga acagttcggt tgtcggcgca
    35401 accttgctgc gggcggcaaa gcgcatcagc tttcgcagct cataaggcgt gccggtagca
    35461 gaaccagaga tgctgcggtc gcccgcaatt aacgtaaagg ccggaacaga cagcggcgtg
    35521 agaaccgcac cgaccgtatg gaaattaccg ccataggtca gcgcctcaaa ataaggctgc
    35581 cagtcgaggc tgacgttcac agtattgata atgagatcaa actgccccga cagggctttc
    35641 agtgcctgcg gatcgcggct attcaccact ttatcggcac ccatcgccag tacttcctgc
    35701 tctttcgccg gattagaact aaaggccgtc acctcgcatc ccattgcgtg cagaagtttt
    35761 atagcgatat gccccagccc gccaatacca attaccccaa cgcggctggt agcagtgata
    35821 tggtgcatca acagtggttt aaagaccgtg ataccgccgc ataacagcgg cccggcggac
    35881 tcgatatcaa tattttctgg cagtggaatc acccattgcc agtccgcacg caacttctcg
    35941 gcaaagccac cgcgattcat aatcgtcggc accgcacctt gctcgcagtt gatctgatta
    36001 ccgctaatac aggcgtcgca gtgaccacag ctacgcgccg tccagccaat cccgacacgc
    36061 tgaccgacct gcaaaccttt atcctgcgcg gcgctcccga gtgccaccac gcgcccaatc
    36121 acctcatgcc cggcaaccag cggatattgt gaaaatcccc attcgttatc gatcatcgac
    36181 agatcggaat gacaaatccc gcagtaatcc acctgcactt caacatcttg tggcttcagc
    36241 tcaccgggat cgtactcata aacttccagt tcgccgcccg cttcttttgc ggcatagctt
    36301 tttatcatcg acatttttgg gtccttctct ggtgttgttt gggaagtgta gagcatggca
    36361 gggcggggtg cctggagtgt gacaaaggtt acatatcgct gagtgtaatg ctgaaaattt
    36421 cagcacttag cgaggtgggt gcaagctggc gcttgcatgg tggcgtgcga caggtataat
    36481 ccacaacgtt ttccgcatac ctcttcagtg ccgaagtggc gaaatcggta gacgcagttg
    36541 attcaaaatc aaccgtagaa atacgtgccg gttcgagtcc ggccttcggc accatcggaa
    36601 catcaataga cgtcaacgga cgtctttttt tgtgcctaaa atccagtatc cgcaaggctt
    36661 tcctcgcttt ttcactcaac ctaagtcaac ctgattcaat ctacatcaac ttactgatgc
    36721 gggtacaact gcgggtattt cccggttcga taatgtttgt acccacacag accccttgaa
    36781 aggatactca tcatggctct gagtgatgtg aaggttcgtt cggctaagcc tgaagcaaaa
    36841 gcctataagc tgaccgacgg tgaaggcatg gttttgctgg ttcaccctaa cggctccaaa
    36901 tactggcggc tacgttatcg ctttggcggc aaagagaaga tgctggcatt ggggaaatac
    36961 cccgaagtgt cgttggcgga tgccagggca cgccgggatg aggctcgtaa gcttttagct
    37021 aatggtgtcg atcccagtga aaacaagaaa gctgttaagg tagaacagga acaagaggcg
    37081 ataacgtttg aagtagtcgc cagagactgg cacgccagca atcagaagtg gtcggcatcg
    37141 catagcgctc gtgttttgaa aagtctggag gataatctct ttactgctat cggtaagcgg
    37201 aacattgcgg aactgaagac gcgggatctt cttgtaccca ttaaagcagt cgagtcatcc
    37261 gggcggctcg aagttgccgc ccgtttacag cagcgtacta ccgcgattat gcgctttgcc
    37321 gttcagagcg gcttaatcga ctacaacccc gcgcaagaga ttgcaggtgc ggttgctacg
    37381 gcgaaaagac agcaccgtgc agctcttgaa ctgaaccgta tacctgaatt acttcatcgc
    37441 atagatcact attctggcag accgttaact cgactggcgg ttgaactcac tttattggtt
    37501 ttcatccgtt cgagcgagct gcgttttgcc cgctggtcag aagtagattt tgaaacggct
    37561 atgtggacaa tcccaggtga gcgtgagcaa ctggaaggag tcaagcattc tcagcgtggc
    37621 tcgaagatgc ggacaccaca ccttgtacct ttgtcgcggc aggccctaag tattttggaa
    37681 aagatcaaaa gcatgagcgg aaatcgagag ctgatttttg tgggcgatca cgatccacgt
    37741 aagccgatga gtgagaatac ggtgaacaag gctttgcgcg taatggggta tgacacgaag
    37801 gtcgaggttt gtgggcatgg cttcaggacg atggcttgta gttcgttgat tgagtcggga
    37861 ttatggtcga gggatgcggt agagcggcag atgagtcacc aggagcgtag ctctgtgcga
    37921 gcagcttaca tccataaggc ggaacatctt ggggagcgta ggttgatgtt tgaagtggca
    37981 cactgaattt ggccacctga acagaggtga tatgctcacc tca
//
LOCUS       DADLSE010000023        36616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLSE010000023 DADLSE010000000
VERSION     DADLSE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.023"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            873..3158
                     /gene="nrdA"
                     /locus_tag="JN692_001182"
     CDS             873..3158
                     /gene="nrdA"
                     /locus_tag="JN692_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7263543.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3392..4522
                     /gene="nrdB"
                     /locus_tag="JN692_001183"
     CDS             3392..4522
                     /gene="nrdB"
                     /locus_tag="JN692_001183"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7263544.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHQTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4522..4776
                     /gene="yfaE"
                     /locus_tag="JN692_001184"
     CDS             4522..4776
                     /gene="yfaE"
                     /locus_tag="JN692_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7263545.1"
                     /translation="MARVTLRITGTQLQCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4830..5486)
                     /gene="inaA"
                     /locus_tag="JN692_001185"
     CDS             complement(4830..5486)
                     /gene="inaA"
                     /locus_tag="JN692_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7263546.1"
                     /translation="MTGDSSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGK
                     KLYVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRA
                     LLVTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRH
                     IYVKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM
                     "
     gene            5648..5890
                     /locus_tag="JN692_001186"
                     /pseudo
     CDS             5648..5890
                     /locus_tag="JN692_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5932..7008)
                     /gene="glpQ"
                     /locus_tag="JN692_001187"
     CDS             complement(5932..7008)
                     /gene="glpQ"
                     /locus_tag="JN692_001187"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7263547.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7013..8371)
                     /gene="glpT"
                     /locus_tag="JN692_001188"
     CDS             complement(7013..8371)
                     /gene="glpT"
                     /locus_tag="JN692_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7263548.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8644..10272
                     /gene="glpA"
                     /locus_tag="JN692_001189"
     CDS             8644..10272
                     /gene="glpA"
                     /locus_tag="JN692_001189"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7263549.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGGRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10262..11521
                     /gene="glpB"
                     /locus_tag="JN692_001190"
     CDS             10262..11521
                     /gene="glpB"
                     /locus_tag="JN692_001190"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7263550.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRVTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFAPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11518..12708
                     /gene="glpC"
                     /locus_tag="JN692_001191"
     CDS             11518..12708
                     /gene="glpC"
                     /locus_tag="JN692_001191"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7263551.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12901..13800
                     /locus_tag="JN692_001192"
     CDS             12901..13800
                     /locus_tag="JN692_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7263552.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPISFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13819..14040
                     /locus_tag="JN692_001193"
     CDS             13819..14040
                     /locus_tag="JN692_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263553.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(14081..14551)
                     /locus_tag="JN692_001194"
     CDS             complement(14081..14551)
                     /locus_tag="JN692_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7263554.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14606..15808)
                     /locus_tag="JN692_001195"
     CDS             complement(14606..15808)
                     /locus_tag="JN692_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7263555.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPPEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGNEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15895..16437)
                     /locus_tag="JN692_001196"
     CDS             complement(15895..16437)
                     /locus_tag="JN692_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263556.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            16716..17141
                     /gene="nudI"
                     /locus_tag="JN692_001197"
     CDS             16716..17141
                     /gene="nudI"
                     /locus_tag="JN692_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7263557.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(17180..17782)
                     /locus_tag="JN692_001198"
     CDS             complement(17180..17782)
                     /locus_tag="JN692_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7263558.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSEGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            18090..19229
                     /gene="arnB"
                     /locus_tag="JN692_001199"
     CDS             18090..19229
                     /gene="arnB"
                     /locus_tag="JN692_001199"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7263559.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19233..20201
                     /gene="arnC"
                     /locus_tag="JN692_001200"
     CDS             19233..20201
                     /gene="arnC"
                     /locus_tag="JN692_001200"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7263560.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSTHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20201..22183
                     /gene="arnA"
                     /locus_tag="JN692_001201"
     CDS             20201..22183
                     /gene="arnA"
                     /locus_tag="JN692_001201"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7263561.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHTARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            22180..23070
                     /locus_tag="JN692_001202"
     CDS             22180..23070
                     /locus_tag="JN692_001202"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7263562.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            23070..24722
                     /gene="arnT"
                     /locus_tag="JN692_001203"
     CDS             23070..24722
                     /gene="arnT"
                     /locus_tag="JN692_001203"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7263563.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            24719..25054
                     /gene="arnE"
                     /locus_tag="JN692_001204"
     CDS             24719..25054
                     /gene="arnE"
                     /locus_tag="JN692_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7263564.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            25054..25440
                     /gene="arnF"
                     /locus_tag="JN692_001205"
     CDS             25054..25440
                     /gene="arnF"
                     /locus_tag="JN692_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7263565.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25434..25706)
                     /gene="pmrD"
                     /locus_tag="JN692_001206"
     CDS             complement(25434..25706)
                     /gene="pmrD"
                     /locus_tag="JN692_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7263566.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25811..27166)
                     /gene="menE"
                     /locus_tag="JN692_001207"
     CDS             complement(25811..27166)
                     /gene="menE"
                     /locus_tag="JN692_001207"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7263567.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(27163..28125)
                     /gene="menC"
                     /locus_tag="JN692_001208"
     CDS             complement(27163..28125)
                     /gene="menC"
                     /locus_tag="JN692_001208"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7263568.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(28125..28982)
                     /gene="menB"
                     /locus_tag="JN692_001209"
     CDS             complement(28125..28982)
                     /gene="menB"
                     /locus_tag="JN692_001209"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7263569.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28997..29755)
                     /gene="menH"
                     /locus_tag="JN692_001210"
     CDS             complement(28997..29755)
                     /gene="menH"
                     /locus_tag="JN692_001210"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7263570.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29752..31422)
                     /gene="menD"
                     /locus_tag="JN692_001211"
     CDS             complement(29752..31422)
                     /gene="menD"
                     /locus_tag="JN692_001211"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7263571.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFGSHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSCLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31511..32806)
                     /gene="menF"
                     /locus_tag="JN692_001212"
     CDS             complement(31511..32806)
                     /gene="menF"
                     /locus_tag="JN692_001212"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7263572.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAALGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAVLRTLLQME"
     gene            complement(32885..33190)
                     /gene="elaB"
                     /locus_tag="JN692_001213"
     CDS             complement(32885..33190)
                     /gene="elaB"
                     /locus_tag="JN692_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7263573.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33245..33706)
                     /locus_tag="JN692_001214"
     CDS             complement(33245..33706)
                     /locus_tag="JN692_001214"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7263574.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33771..34688
                     /gene="rbn"
                     /locus_tag="JN692_001215"
     CDS             33771..34688
                     /gene="rbn"
                     /locus_tag="JN692_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7263575.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34876..36095
                     /gene="elaD"
                     /locus_tag="JN692_001216"
                     /pseudo
     CDS             34876..36095
                     /gene="elaD"
                     /locus_tag="JN692_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         9120 a         9684 c         8949 g         8863 t
ORIGIN      
        1 gatactccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
       61 gatatcatat ccctggcata aatcaatggc cgccgcgaca ccgttagcag agaaaagaga
      121 tgcaatcatt gacgggagta gagataaata ctccttgcgt agaaagataa tccgcatatt
      181 aatctgacca tcattttata taagacggca tatttaaacg cattatacaa tatgggttat
      241 ctttcttttg taaattgtcc aacccgatgc aatggctatt ttgttattat ttgatgaata
      301 atatcagtgc gtcataattc aagttaataa ccttaaggga taccagttat atttaaacta
      361 aattaaagta atgaataatt ttcttataat ataaggtaaa ttaacaaaat ggcttcgcat
      421 ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
      481 tatattgacc acaactgata catcagatta tgtgatgact cgtgcttaga tcaatttttg
      541 caatcattag caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
      601 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
      661 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
      721 agttatgcac ttgcaagagg gtcattttca cactatcttg cagtgaatcc caaacatacc
      781 ccctatatat agtgttctaa gcagcttccc ttactacagg tagtctgcat gaaactattg
      841 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
      901 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
      961 cagaaggact gcataacgtt tcgatttccc aggtcgagct gcgctcccac attcagtttt
     1021 atgacggtat caagacctct gacatccacg aaactattat caaggctgcc gcagacctga
     1081 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcgatc ttccacctgc
     1141 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
     1201 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
     1261 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
     1321 agctggaagg caaatatctg gtacagaacc gcgtgaccgg cgaaatctac gaaagcgccc
     1381 agttccttta tattcttgtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
     1441 tgcaatacgt gaagcgtttt tacgacgcgg tttccacatt taaaatttcg ctgccgacgc
     1501 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
     1561 gcggtgacag cctggattcc atcaacgcca cctccagcgc gattgtgaaa tatgtttccc
     1621 agcgtgccgg gatcggcatc aacgccggac gtattcgtgc gctgggcagc ccgattcgcg
     1681 gtggcgaagc gttccatacc ggctgtatcc cgttctacaa acatttccag acagcggtga
     1741 aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac gctgttctac ccgatgtggc
     1801 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggggttgaa ggcaaccgcg
     1861 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgc ctgctgaaag
     1921 gtgaagatat caccctgttc agcccgtccg acgtaccggg gctgtacgac gcgtttttcg
     1981 ccgatcagga agagtttgaa cgcctgtata ccaaatatga gaaagacgac agcatccgca
     2041 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
     2101 gtcgtatcta tattcagaac gttgaccact gtaataccca tagtccgttt gatccgacca
     2161 tcgcgccagt tcgccagtct aacctgtgcc tggaaattgc cctgccgacc aaaccgctga
     2221 acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
     2281 gcgcaattaa tagcctggat gaactggaag agctggcgat tctggcggtt cgtgcacttg
     2341 acgcgctact ggattatcag gattacccga tcccggccgc caaacgtgga gcgatggggc
     2401 gtcgtacgct gggtattggt gtgattaact tcgcttacta tctggcgaag cacggtaaac
     2461 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcg attcagtatt
     2521 acctgctgaa agcgtctaat gagctggcta aagagcaagg cgcgtgcccg tggtttaacg
     2581 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatgcca
     2641 tcgctaatga gccgctgcat tacgactggg aagcattgcg tgaatcaatc aaaacgcacg
     2701 gcctgcgtaa ctccacgctt tctgccctga tgccgtccga gacctcttcg cagatctcta
     2761 acgccactaa cggtattgaa ccgccgcgcg gttacgtcag catcaaagcg tcgaaagacg
     2821 gtattttgcg ccaggtggtg ccggactacg agcacctgca cgacgcctat gagctgctgt
     2881 gggaaatgcc gggtaacgat ggctacctgc aactggtagg tatcatgcag aaatttatcg
     2941 atcagtcgat ctctgctaac accaactacg atccgtcacg tttcccgtca ggaaaagtgc
     3001 cgatgcagca gttgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
     3061 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
     3121 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
     3181 taaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
     3241 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     3301 taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     3361 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     3421 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     3481 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     3541 tccggaagaa gttgacgtct cccgcgaccg tatagattac caggcgctgc cggagcacga
     3601 aaaacacatc tttatcagca acctgaaata tcagacgctg ctggattcca ttcagggtcg
     3661 tagcccgaac gtggcgctat tgccgcttat ttctattccg gaactggaaa cctgggtcga
     3721 aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     3781 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     3841 tgcggaaggg atctccagct attacgatga actgatcgaa atgaccagct actggcacct
     3901 tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta accgttagcc tgcgcgagct
     3961 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga tccgcttcta
     4021 cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag gtaacgccaa
     4081 gattattcgc ctgattgccc gcgatgaagc cctgcaccag accggtactc agcatatgct
     4141 gaatctgctg cgcagcggcg cggatgatcc ggagatggcg gaaattgccg aagaatgtaa
     4201 gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact gggcggatta
     4261 tctgttccgt gacggttcga tgattggtct gaataaagat attctctgcc agtacgttga
     4321 atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     4381 caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     4441 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcggaagtgg acaccgacga
     4501 tttgagtaac ttccagctct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     4561 cagtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgtggcggtt
     4621 gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cccgactggt cgcaggtcag
     4681 gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     4741 tgccgggcaa aaggcgatat tgaaatcgag ttgtgatcgg gtactagcgc cagatacgac
     4801 gcttacgcgt attatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     4861 tcccagtccg ctttagggat cggctccaga tatttttcca gctggcggaa gtcatgatta
     4921 atcgctttat cgcgacgcaa gcggcgacga cttttctcca gatcaagaaa accagcttct
     4981 gccttacctt ctgttttcac atagatatgg cgaacatagc aacagccatg ctgacgatta
     5041 acgctatgca ttttcttaaa cgccagtgcc actgctttca acatggcttg ccgtacttcg
     5101 tcagaataag gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     5161 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     5221 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     5281 acaatcgttg gtcggccgaa cggataacgt actgaatgaa acagatgatg cgtcatgcgc
     5341 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     5401 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5461 tactttgctg aactatcgcc tgtcattttt attaacgaca atgactatag gtggttacct
     5521 gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttt ttttgccgct acaggtgtga
     5581 caccgctttt gcccttcatt tgcagcgcat agattccacc agccactacc actaatagtg
     5641 cgcccagcag ataaaatgtg aatttaatca gtcggataca gcgatgaatt ttattcgaca
     5701 gggattaggt atcgccttac aaccagagtt aacgctgaaa agcattgcag gtgaattgtg
     5761 ttccgttcct cacgaaccaa ccttctatcg acagatttcg ttgctgacta aagaaaagcc
     5821 ggtagaaggc agtccactgt ttttactaca aatgtgcatg gaacaattag tggcgattgg
     5881 aaaaatttga tatgagacag gatggcgcat gaacgccatc ctgcagtaac attactcttt
     5941 attaagaaat tttactgcct tatcagggaa atcagtaaac agcccattta cacccgcttt
     6001 gttatacaga gcatcatata actgattcac atcaggagtg tattcaggca gtttatctga
     6061 ccgcacggta taaggatgca ctaccagttt attctgctga gcatcttgca ccatgccagt
     6121 gagtttgata ttacccggct gcgatgtctc ctcaatcaac atatggtaat ccggaccaat
     6181 accatctgca tattccgcca cctgtttcat ggcacccggc ttaaacatcc agtcgtagtt
     6241 gtaattaacc cagcttccat ccggctgttt ctgctgcgtt tcattccagt cggtataggc
     6301 aatcagctgt accagattga gctccatgcc cattttgggt tccagctcat tcttaatacg
     6361 cttcagctca tcagcatcaa aacattgcaa ataaacttta tcgtctttac cggtgtaacc
     6421 atatttcttc agcacttcca gcgtttttgc cgcaatatcc ttcccttcct gatgatggaa
     6481 ccacggcgct ttgatttctg gatagatacc gatatttttc ccggtagagt gatttaaccc
     6541 ctgaacaaat tcaatctctt cttcaaaggt gtgcacccgg aagtcggact tacccattgg
     6601 gaaacgcccc ggataagtct gtactttttt accgttttca atatcgaaac cttcggtaaa
     6661 tttcagcgac ttaatttcat ccagcgtgaa atctatcgcg tagtaacgac cgtctttgcg
     6721 cgcccgatcc gggaaacgat cggcaacatc agtaacacga tcgagataat ggtcatgcag
     6781 aacaaccaga tggtcgtctt tggtcatcac caaatcctgt tccagataat ccgctccctg
     6841 cgcatacgcc atcgcttttg ctggcagcgt atgctccggc aaatatccac tggcaccgcg
     6901 atgggcgatg actatttttt cgttgctgtc cgccgccatt gcactgcttc ccatgactat
     6961 agtgctcatc atgatcgcca tgctaaggtt tttcagcttc aatttcatgc cattagcctc
     7021 cgttgcgttt ttgcagtaat tgttcatggc gacgtttttc gccaatcatc acaacaatca
     7081 acaagataac cgccagaatg ctgccgccaa tcattaccat aaagccgcca tcccagccga
     7141 agaagtccac ggtgtagcca acaatcgcgc tcgccgccac cgaaccgccc aggtaaccaa
     7201 acagcccggt aaagcccgct gccgtacctg ccgctttttt cggtgccagt tccagcgcat
     7261 gcagaccgat cagcatcaca ggaccgtaga tcaggaagcc gataacaatc atacaaatca
     7321 tatcgacggt tgggttacct gccgggttca tccagtaaac gatagtcgcg atggtcacca
     7381 gtgtcataaa gaagacgccg gttgccccac ggttaccacg gaagactttg tccgacatcc
     7441 agccgcacag cagggtgccc ggaatacccg catactcata gaagaagtag gcccaggagg
     7501 atttatccag cgcgaaatgc ttaacctctt tcagataagt cggtgaccag tcgaggatgc
     7561 cgtaacgcag cagataaacg aacacgttgg cgatggcgat gtaccacagc agtttgttcg
     7621 gcagcacgta ctgcatgaag atttgcttcg cggtcagctc ctgttccgct ttttcgttat
     7681 agtcgtccgg ataatcattt ttgtactctt cgatcggcgg caagccacag gattgcgggg
     7741 tatcgcgcat catcgcaaag gcgaataatg ccaccagaat ggcgcagaaa gcaggcatat
     7801 agagcgccgc atgccagtca ttgaaccagg ccatccccag caggaacagc agcggcggaa
     7861 taccaccacc gacgttgtgc gcacagttcc acactgacac aatgccgcca cgttctttct
     7921 gcgaccacca gtgcaccata gtacgaccac acggcggcca ccccatcccc tggaaccaac
     7981 cgcagaggaa caacagtaca aacatcaccg caatgctcga cgtcgcccat ggcacaaagc
     8041 ccataaacaa catcactgcc gccgccagaa tcaaacctgc gggcaggaaa acgcgcggat
     8101 tcgagcgatc cgataccgaa cccatgatga atttcgaaaa tccataagca atcgagatcc
     8161 ccgaaagggc aaaacctaaa tcaccgcgtg agaatccctg ctcaaccaga taaggcatag
     8221 caagcgcaaa gttcttacga accaaatagt aagccgcata gccaaagaat atccccagga
     8281 aaatttgcca gcgcaatcga cgataagtcg gatcgatctc cgcggcaggt aagcgcgctt
     8341 tgtgtggcgc tggtttaaaa atactcaaca ttgatagcct ccgtggcccg tggtcttatt
     8401 tatgattaac agcctgattc agtgagagaa cctgccgttt cttgagttgc cgcgatgtta
     8461 agaaaacatt cataaattaa atgtgaattg ccgcacacat tattaaataa gatttacaaa
     8521 atgttcaaaa tgacgcatga aatcacgttt cactttcgaa ttatgagcga atatgcgcga
     8581 aatcaaacaa tttatgtttt tactatggct aaatggtaaa aaacgaactt cagagggata
     8641 acaatgaaaa ctcgcgactc gcaatcaagt gacgtgatta tcattggcgg cggcgcaacg
     8701 ggagccggga ttgcccgcga ctgtgccctg cgcgggctgc gcgtgatttt ggttgagcgc
     8761 cacgacatcg caaccggtgc caccgggcgt aaccacggcc tgctgcacag cggtgcgcgc
     8821 tatgcagtaa ccgatgcgga atcggcccgc gaatgcatta gtgaaaacca gatcctgaaa
     8881 cgcattgcac gtcactgcgt tgaaccaacc aacggcctgt ttatcaccct gccggaagat
     8941 gacctctcat tccaggccac ttttattcgc gcctgcgaag aagcagggat cagcgcagaa
     9001 gctatagacc cgcagcaagc gcgcattatc gaacctgccg ttaacccggc actgattggc
     9061 gcggtgaaag ttccggatgg caccgttgat ccatttcgtc tgaccgcagc aaacatgctg
     9121 gatgccaaag aacacggtgc cgttatcctt accgctcatg aagtcacggg gctgattcgt
     9181 gaaggcgcga cggtgtgcgg tggtcgtgta cgtaaccatc tcaccggcga aactcaggcc
     9241 cttcatgcac ctgtcgtggt taatgccgct gggatctggg ggcaacacat tgccgaatat
     9301 gccgatctgc gcattcgcat gttcccggcg aaaggatcgc tgctgatcat ggatcaccgc
     9361 attaaccagc atgtgatcaa ccgctgccgt aaaccttccg acgccgatat tctggtgcct
     9421 ggcgatacca tttcgctgat tggtaccacc tctttacgta ttgattacaa cgagattgac
     9481 gataatcgag tgacggcaga agaggttgat attctgctgc gtgaagggga aaaactggcc
     9541 cccgtgatgg cgaaaacgcg cattttgcgg gcctattctg gcgtgcgccc gctggttgcc
     9601 agcgatgacg acccgagcgg acgtaacgtc agccgtggca tcgtgctgct cgaccatgct
     9661 gaacgcgatg gtctggacgg atttatcacc atcaccggtg gcaaactgat gacctatcgg
     9721 ctgatggctg aatgggctac cgacgcggta tgccgcaaac tgggcaacac gcgcccctgt
     9781 acgactgccg atctggcact gcctggttca caagaacccg ctgaagttac cttgcgtaaa
     9841 gtcatctccc tgcctgcccc gctgcgcggt tctgcggttt atcgtcatgg cgatcgcacg
     9901 cctgcctggc tgagcgaagg ccgtctgcac cgtagcctgg tatgtgagtg cgaagcggta
     9961 actgcgggtg aagtgcagta cgcggtagaa aatttaaacg ttaatagcct gctggattta
    10021 cgccgtcgta cccgtgtggg gatgggcacc tgccagggcg aactctgcgc ctgccgcgct
    10081 gccggactgc tgcaacggtt taacgtcacg acgtccgcgc aatctatcga gcaactttcc
    10141 accttcctta acgaacgctg gaaaggcgtg caacccatcg cctggggaga cgcactgcgc
    10201 gaaagcgaat ttacccgctg ggtttatcag ggactgtgtg gtctggagaa ggagcagaaa
    10261 gatgcgcttt gatactgtca ttatgggcgg cggcctcgcc ggattactct gtggcctgca
    10321 actgcaaaaa cacggcctgc gctgtgccat tgtcacgcgt ggtcaaagcg cgttgcattt
    10381 ctcatccggt tcgctggatt tgctgagcca tctgccagat ggtcaacccg tggcagacat
    10441 tcacagtgga ctggaatctt tgcgtcagca ggcacctgca catccttact ctcttctcgg
    10501 gccacaacgc gtgctcgatc tcgcttgcca ggcgcaggca ttaatcgctg aaagcggtgc
    10561 gcaattgcag ggcagcgtag aactggctca ccagcggatt acgccgctcg gcactttgcg
    10621 tgtaacctgg ttaagttcgc cagaagttcc cgtctggccg ctacccgcga agaagatatg
    10681 tgtagtgggg ataagcgggc tgatggattt ccaggcgcat cttgcggcgg cttcgttgcg
    10741 cgaactggat ctttcggttg aaaccgcaga aatagagctg ccggaactgg atgtcctgcg
    10801 caataacgcc accgaatttc gcgcggtgaa tatcgcccgt ttccttgata atgaagaaaa
    10861 ctggccgctg ttacttgatg cgcttattcc tgtcgccaat acctgcgaaa tgatcctgat
    10921 gcccgcctgc ttcggcctgg ccgatgacaa actgtggcgt tggttgaatg aaaaactacc
    10981 ttgttcactg atgcttttgc caacgctgcc gccttccgtg ctgggcattc gtctgcaaaa
    11041 ccagttacag cgccagtttg tgcgccaggg tggcgtgtgg atgccgggcg atgaagtgaa
    11101 aaaagtgacc tgtaaaaatg gcgtagtgaa tgaaatctgg acccgcaatc acgccgatat
    11161 tccgctacgt ccacgtttcg cggttctcgc cagcggcagt ttctttagtg gcggactggt
    11221 agcggaacgt aacggcattc gcgagccgat tctcggcctt gatgtgctac aaaccgccac
    11281 gcggggtgaa tggtataagg gcgatttttt tgcgccgcaa ccgtggcagc agttcggtgt
    11341 aaccactgat gagacgctac gcccgtcgca ggcagggcaa accattgaga acctgtttgc
    11401 catcggttcg gtgctgggcg gatttgctcc catcgcccag ggatgcggcg gcggtgtttg
    11461 tgccgtcagt gctttacatg ccgctcaaca gattgcccaa cgcgcaggag gccaacaatg
    11521 aacgacacca gcttcgaaaa ctgcattaag tgcaccgtct gcaccaccgc ctgcccggtg
    11581 agcagggtga atcccggtta tccagggcca aaacaagccg ggccggatgg cgagcgtctg
    11641 cgtttgaaag atggcgcact gtatgacgag gcgctgaaat attgcatcaa ctgcaaacgt
    11701 tgtgaagtcg cctgcccgtc cgatgtgaag attggcgata ttatccagcg cgcgcgggcg
    11761 aaatatgaca ccacgcgccc gtcgctgcgt aattttgtgt tgagtcatac cgacctgatg
    11821 ggtagcgttt ccacgccgtt cgcaccaatc gtcaacaccg ctacctcgct gaaaccggtg
    11881 cggcagctgc ttgatgcggc gttaaaaatc gatcatcgtc gcacgctacc gaaatactcc
    11941 ttcggcacgt tccgtcgctg gtatcgcagc atagcggctc agcaagcaca atataaagac
    12001 caggtcgctt tctttcacgg ctgcttcgtt aactacaacc atccgcagtt aggtaaagat
    12061 ttaattaaag tgctcaacgc aatgggtacc ggtgtacaac tgctcagcaa agaaaaatgc
    12121 tgcggcgtac cgctaatcgc caacggcttt accgataaag cacgcaaaca ggcaattacg
    12181 aatgtagagt cgatccgcga agctgtggga gtaaaaggca ttccggtgat tgccacctcc
    12241 tcaacctgta catttgccct gcgcgacgaa tacccggaag tgctgaatgt cgacaacaaa
    12301 ggcttgcgcg atcatatcga actggcaacc cgctggctgt ggcgcaagct ggacgaaggc
    12361 aaaacgttac cgctgaaacc gctgccgctg aaagtggttt atcacactcc gtgccatatg
    12421 gaaaaaatgg gctggacgct ctacaccctg gagctgttgc gtaacatccc ggggcttgag
    12481 ttaacggtgc tggattccca gtgctgcggt attgcgggta cttacggttt caaaaaagag
    12541 aactacccca cctcacaagc catcggcgca ccactgttcc gccagataga agaaagcggc
    12601 gcagatctgg tggtcaccga ctgcgaaacc tgtaaatggc agattgagat gtccacaagt
    12661 cttcgctgcg aacatccgat tacgctactg gcccaggcgc tggcttaaac tcccttctga
    12721 tgcccggtaa gcatgtggtt accgggcatt tttgcgtaca cgattccgtg cccaatgtat
    12781 gcgttgcaac gcagtgaaaa ttcctctgaa aacgtctcgc aaaggctgaa actggcagat
    12841 gtcaaaggcc tgggataacc gtaatgtcgc gtcatcataa atatcaggtg acggacaacc
    12901 atgaccgaat caacaacctc ctccccgcat gatgcggtat ttaaaacctt tatattcaca
    12961 cccgaaaccg cacgggattt tctcgaaata catttaccag aaccactgcg caagctttgc
    13021 aacctgcaaa ccttacgcct ggaacccatt agttttattg aaaaaagttt acgcgcttac
    13081 tactcggatg ttttgtggtc cgtggaaacc agcgacggtg acggctatat ctactgcgtg
    13141 attgaacatc aaagctctgc agaaaagaat atggcttttc ggccaatgcg ctatgccact
    13201 gccgccatgc agcgtcacct agataaaggc tatgacagag ttccgctggt ggtgccattg
    13261 ctgttttatc atggcgaaac atcgccctac ccgtactcac ttaactggct ggatgagttt
    13321 gacgatccgc aacttgcccg gcagttgtac accgaagctt ttccgttggt ggatatcacc
    13381 atcgtacctg acgatgagat catgcaacat cggcgtatag ctctgctgga actgattcaa
    13441 aagcatattc gcgaccacga tttaatcggc atggtcgaca ggatcaccac gcttttggtt
    13501 agaggcttca ctaatgacag ccagctacaa acactgttta attatctgct gcaatgcggc
    13561 gatacctccc gtttcacccg ttttattcag gagattgccg aacgttcacc actacaaaag
    13621 gagagattaa tgactattgc tgaacggcta cggcaggaag ggcatcaaat tggctggcag
    13681 gaaggtatgc atgaacaagc cattaaaatt gccttgcgca tgctggagca gggctttgaa
    13741 cgtgagattg tgctggcggc aacccaactc actgatgctg atattccgaa ctgttattaa
    13801 aaggagaggg agagattgat gactattgct gaacggctac ggcaggaagg acatcaaatt
    13861 ggctggcaag aaggtaaatt agtaggtttg cagcaaggta aattagaagg tttgcaggaa
    13921 ggtatgcatg aacaagccat taaaattgct ttgcgcatgc tggaacaggg cattgatcgt
    13981 gaccaggtgc tcgcggccac ccagctaagc gaagccgatc tggcagcgaa taaccactaa
    14041 ttaacacagg cccacagccg atccccatgg gcctttgata tcaataacta ccttttatgc
    14101 gtggcccatt tttgcctgat ttaaacatcg ccagtcgttg atccagggca tcgctgtaga
    14161 gcatcgtgtc aacgccaaca gcgacaaagt tcgctcccca cgccaggcat tgctgcgcca
    14221 tatcaggagc cacagccaga aaaccagccg ctttacccgc agcacggatc cgccgaatgc
    14281 tggtttcaat aattcgctgc acttccgggt gcccggcgtt atccgggtag cccaacgacg
    14341 cagaaagatc cgctggtcca ataaacacgc catcaatccc ttcgacgtcg aggatctcgt
    14401 ccaggttatc cagtgccgtt ttactttcca cctgcactaa cagacaaagc gaatcgttga
    14461 cttgcgccat gtaattctca atgcgtcccc agcgcgcagc ccgtgccaca ctggccccaa
    14521 caccacgctc accgtaggga gggttggaca aattttgccg gagggggaat gtgccggtgc
    14581 agtaaaccgg cacagagtgg cgaaatcaga cggataaagt catggactca acaacctcaa
    14641 tccagccatg ctcactagcg atgtcctggc cgtttaacca acggcgtagc atattcagtg
    14701 ccatcattgc gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca
    14761 cacgcagagc gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga
    14821 aacccgaaac agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt
    14881 gcgcagtttg cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag
    14941 cacctgcgcg agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca
    15001 ggctgaactg gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcgttgcctt
    15061 cgaaaatcac gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct
    15121 gttgctcgct tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc
    15181 ccattgttac gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg
    15241 aacgaccaaa ggtagttaaa cgcagacaaa ccggcggctg tggtaaagaa aaacgctcgc
    15301 gcaggcgcgg caggatttcg tgctcgacca tcaccttaaa ttctggcggt acgccgggag
    15361 taaagaacat caggcaacga ttaagctgca cggcaaaacc acaggcggtg cctaccgggt
    15421 tattgataaa ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta
    15481 ctcgtccacg ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca
    15541 ggccttcacc ttttgctgtc gcagcggcga gtgcgcttaa atcatcgctg gtcggtccca
    15601 gcccgccgtt aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta
    15661 agtcatcaag gttatccccc accgtattgc ggcgagataa tggcagcccc tgatgaaaga
    15721 aaaaatcggc cagccaggca gcgttagtgt caacgatttg cccgtataac acttcatccc
    15781 cggtggatag catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta
    15841 taacgcaaag ggagataagg cgtagtaaga aactgaatgc cgcgccggaa gggatcagaa
    15901 actggcgtta accccaacat acgggccgtc agccacggcg ttgtcgcggt taccgtcttt
    15961 acccgacaga ttcaggtagc gataacccgc ctcaatactg actggacgca taatggtgta
    16021 acgcgcgcca gcattcgctt cctcataact ttgaataccg ctggagagcg aatccggaga
    16081 gtagtaatac tcgccaaaca aacggaagct gttgccaatt ttccactgca aaccacctcc
    16141 taccgctgcg gcataacctt catcgccgta attcgggttg gtgtacacgc cttttccgcc
    16201 aacggtcgcc attaacggcc cgagaggcag attcaacccc agccccacgc ccgcgacgtc
    16261 accgtcgtcg tcgttatgtg tccagttacc gcttaaagcc aggcccgtcg attcagtccc
    16321 aaaaccgaca ccaatattgg tgtattcttt acccgcctgg ccgctgatgc tcattgcatt
    16381 gaccgatgca gaaaccaaca gcattccggc cagacctgct agcgcaattt ttttcattat
    16441 cccctttccc acaagcaatt aatcaaaatt cccaaagctg cgggattgta ctgcctggca
    16501 cagaggattc aatgagcgaa aaaagggatc tagtcactta ttgtaactaa atacttctta
    16561 aataattaaa cgccgaaaaa ataaacacac tttaccgata cacacaaaaa taataataag
    16621 tgcagttatc attgcaaaag agattactgt cactttcaca tatttctgag ttaccgtatt
    16681 ctcatggttt ctttttattt ataaggaata cccctgtgcg acaacggact attgtatgcc
    16741 ctttgattca aaatgatggt gcttatttgc tgtgcaaaat ggccgacgat cgcggcgttt
    16801 tccccggtca atgggcgctt tcgggtggcg gcgtggagtc tggcgaacga attgaagagg
    16861 cactacgccg cgaaattcgc gaagaactgg gagaacagct gcttttgaca gaaatcacgc
    16921 cgtggacctt cagcgatgat attcgcacca agacatatgc agatggtcgc aaggaagaga
    16981 tttatatgat ttacctgatt tttgactgcg tttctgccaa ccgagaagtg aaaataaacg
    17041 aagagtttca ggactacgcg tgggtaaaac ctgaagatct ggtgcattat gatttaaatg
    17101 ttgccacccg aaaaacgtta cgtttgaaag gtcttcttta acaatgatga cagccatcac
    17161 tggctgtcag attgaaaatt tagtggttaa cgaattcccc atccagataa actttgcctt
    17221 tttccacatg catgactaaa ccatccagat aatcaggttt aaatgtcgca tcacgcttat
    17281 ttttagcaat atatgtcagg caatgattat gggtgaaaat aacgatattt ttatcaggcg
    17341 ctttgctttg taagtcctta attgcactat aaatctcatt accgcactga agaagtcgtt
    17401 tatctaccgt cagcttttta ccctcggaaa accaggtcgc cgactgaatg gtccggacgg
    17461 tattactgga ataaagatcg aaatcaggga tgtcagcgct aaaagcgttg cccagttcac
    17521 gggcatcctg ggtaccttta accgtaatac ctgttttatc tgacaagcat tgattggttg
    17581 aacgatcgca acgttcagca tggcgaaaca aaacgacaac cgggtgctgc tgtgccagtc
    17641 tggccaatgt tttattgtcg atacgtggca aaccattgct actccaggcg gcatgagtac
    17701 ccagtccggc aattgcagcg agcgccagta aaatgatgaa atattttttt gacttcaatg
    17761 atgagcggca aaaagctaac acgtaaactc cacctataga caagcgcaac cagacaatta
    17821 ccgtgaaatt gagcgacatt tctggcgata attagcagtt ggtgtaatat taaaaatcct
    17881 acgatgtcgg caaaatgcct caaatttttg ccaaatgcaa agcctaaata agaaaaaata
    17941 taaaaatttc aatatttacg tctaatatta gtttcttaag gttaagttaa tattctatcc
    18001 ttaaaatttc gctccaaatg gcaaaatata cacaacactc tttatagcaa atataagtgg
    18061 acaggtattc aatggcggaa ggaaaagcaa tgtcagaatt tttgcctttt tcgcgaccag
    18121 caatgggcgt ggaggaactc gctgcagtta aagaggttct cgaatccggt tggatcacaa
    18181 ccggtccgaa gaatcaggcg cttgagcaag ccttttgcca gttgacggga aatcagcatg
    18241 ccatcgcggt cagttcagcc accgccggaa tgcatatcac gctaatggcg ttggaaattg
    18301 gcaagggcga tgaagtgatt acgccttccc tgacctgggt ttcaaccctc aatatgattt
    18361 ccttgctggg tgcaacgccg gtaatggtgg atgtcgaccg cgatacgctg atggtcacgc
    18421 ctgaagcgat cgaatcagcc attacgccac gcactaaagc catcattccg gtgcattatg
    18481 ccggtgcgcc agcagatatt gacgccattc gcgccattgg cgaacgttac ggcatcgcag
    18541 ttatcgaaga tgctgcccat gccgtcggta cgtattacaa agggcgacat attggcgcaa
    18601 aaggtaccgc tattttttca tttcatgcca ttaaaaatat tacctgtgct gaaggtggcc
    18661 tgattgtaac tgataatgaa aaccttgccc gccagctacg gatgctgaaa tttcacggtc
    18721 tgggtgtcga tgcctatgac agacaaacct ggggccgtgc accgcaggct gaagtcttaa
    18781 caccgggcta taagtacaat ctgaccgata ttaacgccgc gattgccctg acacagttag
    18841 tcaaattaga gcacctcaac acccgtcggc gcgaaattgc ccagcaatat cagcaagcac
    18901 tggcagctct cccctttcag ccattaagcc ttcccgcctg gccgcacgtt cacgcctggc
    18961 atctgtttat tattcgtgtc gatgaacaac gttgtggtat cagtcgcgat gcgttgatgg
    19021 aagcgttaaa agaaagaggc attggtaccg ggttacattt ccgcgccgct cacacacaaa
    19081 aatattatcg cgagcgtttt cccacgctgt cgttaccgaa taccgaatgg aatagcgaac
    19141 gcatctgttc gttgccgctg ttcccggata tgactaccgc cgatgccgac cgcgtcatca
    19201 cagcccttca gcaactcgca ggacaataag ccatgtttga aatccaccct gttaagaaag
    19261 tctcggtggt tattcccgtt tataacgagc aggaaagctt accggaatta atcaggcgca
    19321 ccaccacagc ctgtgaatcg ttggggaaag agtatgagat cctgctgatt gatgacggca
    19381 gtagcgataa ttccacgcat atgctggtcg aagcctcaca agcggagaac agccatattg
    19441 tgtctatttt gcttaaccgc aattacgggc aacattcagc gattatggcg ggattcagtc
    19501 acgttactgg cgacttaatt attacccttg atgccgatct ccagaatccg ccagaagaaa
    19561 tcccccgcct ggtggcaaaa gccgatgaag gttacgacgt ggtagggact gtacgccaga
    19621 accgccagga cagctggttt cgtaaaacag cttcgaagat gattaaccgg cttattcagc
    19681 gcaccactgg caaagcgatg ggtgactacg gttgtatgct gcgcgcctat cgccgtcata
    19741 ttgtcgatgc gatgttgcac tgccatgaac gcagcacctt tatcccgatt ctggcgaata
    19801 tcttcgcccg ccgtgcaatt gaaattccag tacatcatgc cgagcgtgag tttggtgaat
    19861 ccaaatacag ttttatgcgc ctgattaatt tgatgtacga cctggtgacc tgccttacca
    19921 caacgccgct acgtatgctg agtctgctcg gcagcattat tgcgattgga ggttttagca
    19981 ttgcggtgct gctggtgatt ttacgcctga ccttcggacc acaatgggcg gcagaaggcg
    20041 tctttatgct atttgccgtg ctgtttactt ttattggcgc tcagtttatc ggcatgggat
    20101 tactcggtga atatatcggc aggatctaca ccgatgtccg cgcccgcccc cgctattttg
    20161 ttcagcaagt tatccgtcca tccagcaagg aaaatgaata atgaaaaccg tcgtttttgc
    20221 ctaccacgat atgggatgcc tcggtattga agctctgctg gctgccggtt acgaaattag
    20281 cgccattttt acccataccg ataatcccgg tgaaaaagcc ttttatggtt cggtggctca
    20341 tctggcggcg gaaagaggca ttccggttta tgcgccggat aacgttaatc atccgctgtg
    20401 ggtggaacgc attgcccaac tgtcaccaga ggtgattttc tctttttatt atcgccatct
    20461 tatttgcgac gagattttgc agctcgctcc cgcaggtgca tttaatctgc acggttcgct
    20521 gttaccaaaa tatcgtggtc gcgcgccgct gaactgggtg ctggtgaacg gtgaaacgga
    20581 aacgggcgtt acattgcacc gaatggtaaa acgtgccgat gctggggcca ttgtggcgca
    20641 actgcgcgtt gccattgcgc cagacgatat cgctattacg ctgcatcata aattgtgcca
    20701 taccgcgcgc cagctactgg aacagacatt acccgccatt aaacacggta atattctgga
    20761 aatcgcccag cgcgaaaacg aagccacctg ttttggtcgc agaacgccgg atgacagttt
    20821 ccttgaatgg cataaaccgg catccgtact gcacaacatg gtacgtgccg ttgccgatcc
    20881 gtggccgggt gccttcagct atgttggcaa tcagaaattc accgtctggt cgtcgcgtgt
    20941 tcatcctcat gccagcaaag cacagccggg gagcgtgatt tctgttgcgc cactgctgat
    21001 tgcctgtggc gatggcgcgc tggaaatcgt caccggacag gcgggcgacg gcattactat
    21061 gcagggctcg caattagcgc agatgctggg cctggtgcaa ggttcacgct tgaatagcca
    21121 gcctgcctgc accgcccgac gccgtacccg ggtactcatc ctcggggtga atggctttat
    21181 tggcaaccat ctgacagaac gcctgctgcg cgaagatcat tatgaagttt acggtctgga
    21241 tattggcagc gatgcgataa gccgttttct gaatcatcca cattttcact ttgtcgaagg
    21301 cgatatcagt attcattccg aatggattga gtatcacgtc aaaaaatgtg atgtcgtctt
    21361 gccgctggtg gcgatagcca cgccgattga atatacccgc aacccgctgc gcgtatttga
    21421 actcgatttt gaagagaatc tgcgcattat ccgctactgc gttaagtacc gtaagcgaat
    21481 catcttcccg tcaacttcag aagtttatgg gatgtgtagc gataaatact tcgatgagga
    21541 ccattctaat ttaatcgtcg gcccggtgaa taaaccacgc tggatttatt cggtgtcaaa
    21601 acaattactt gatcgggtga tctgggccta tggcgaaaaa gagggtttac agttcaccct
    21661 cttccgcccg tttaactgga tgggaccacg actggataac cttaatgcag cgcgaattgg
    21721 cagctcccgc gctattacgc aactcattct caatctggta gaaggttcac caattaagct
    21781 aattgatggc ggaaaacaaa aacgctgctt tactgatatc cgggacggta tcgaggcgtt
    21841 gtaccgcatt atcgaaaatg cgggaaatcg ctgcgacggc gaaattatca acattggcaa
    21901 tcctgagaac gaagcgagca ttgaggaact gggcgagatg ctgctggcga gcttcgaaaa
    21961 acatccgctg cgccatcatt tcccaccgtt tgcgggcttt cgcgttgtcg aaagtagcag
    22021 ctactacggc aaaggatatc aggacgtaga gcatcgtaaa ccgagcatcc gcaatgccca
    22081 ccgctgcctg gactgggagc cgaaaattga tatgcaggaa accatcgacg aaacgctgga
    22141 tttcttcctg cgcaccgttg atcttacgga taaaccatca tgaccaaagt aggcttacgc
    22201 attgatgtcg atacctttcg tggcacccgt gaaggcgtgc cgcgtctgct ggaaatcttg
    22261 agtaagcata atattcaggc cagcattttt ttcagcgtcg gcccggacaa tatgggccgc
    22321 catctctggc gactggtgaa gccacagttt ttgtggaaga tgctgcgctc aaacgcggca
    22381 tcgctttatg gctgggatat tttactggca ggtacggcct ggccaggtaa agagattggt
    22441 catgccaatg ccgatatcat tcgtgaagcg gctaaacatc acgaagtcgg cctgcacgcc
    22501 tgggatcacc atgcctggca agcccgtagc ggtaactggg atcggcaaac aatgatcgac
    22561 gatattgcac gcggtcttcg cactctggaa gagattatcg gtcaaccggt aacctgttct
    22621 gccgctgcgg gctggcgtgc cgaccagaag gtgatcgaag caaaagaagc gttccatttg
    22681 cgctacaaca gcgattgtcg tggggccatg ccgttccgtc cactgctcga atcaggaaac
    22741 cctggcactg cgcaaattcc ggtgacctta cccacctggg atgaagtgat tggtcgggat
    22801 gtgaaagcag aagattttaa cggttggtta ctcaaccgca tcctgcgaga taaaggcacg
    22861 ccggtttata ccattcatgc agaagttgaa ggctgcgctt atcagcataa ttttgtggat
    22921 ctcctcaaac gcgcagctca ggaaggcgtg acattttgcc ctttaagcga actgttatca
    22981 gagacgttgc cgctcggaca agttgttcgc ggaaatattg ccggacgtga aggctggctg
    23041 ggttgccaac aaattgcggg tagtcgctga tgaaatcggt acgttacctt atcggcatct
    23101 tcgcgtttat tgcctgctat tacctgttac cgatcagcac gcgtctgctc tggcaacccg
    23161 atgaaacgcg ttatgcggaa atcagtcgag aaatgctggc atccggcgac tggattgtgc
    23221 cccatctgtt agggctacgt tatttcgaaa aacccattgc cggatactgg attaacagca
    23281 ttgggcaatg gctatttggc gcgaataact ttggtgtgcg ggcaggcgtt atctttgcga
    23341 ccctgttaac tgccgcgctg gtgacctggt ttactctgcg cttatggcgc gataaacgtc
    23401 tggctctact cgccacagta atttatctct cattgtttat tgtctatgcc atcggcactt
    23461 atgccgtgct cgatccgttt attgcattct ggctggtggc gggaatgtgc agcttctggc
    23521 tggcaatgca ggcacagacg tggaaaggca aaagcgcagg atttttactg ctgggaatca
    23581 cctgcggcat gggggtgatg accaaaggtt ttctcgccct tgccgtgccg gtattaagcg
    23641 tgctgccatg ggtagcgacg caaaaacgct ggaaagatct ctttatttac ggctggctgg
    23701 cggttatcag ttgcgtactg acggttctcc cctggggact ggcgatagcg cagcgggagc
    23761 ctgacttctg gcactatttt ttctgggttg agcatattca acgctttgca ctagatgatg
    23821 cccaacatag agctccgttc tggtactacg tgccggtcat cattgccggt agcctgccgt
    23881 ggctgggatt actccccggt gcactgtaca caggctggaa aaaccgcaag cattccgcaa
    23941 ccgtctattt gttgagctgg acgataatgc cgctgctgtt tttctccgtc gctaaaggca
    24001 aattgcccac ctatattctt tcttgctttg cacctctggc aatgctgttg gcgcattacg
    24061 ctttgctggc agcaaaaaat aatcctctgg cgctgcggat taatggctgg attaacatcg
    24121 cttttggcgt cactggcatt attgccacat ttgtgatctc cccctgggga ccaatgaaca
    24181 cgccggtgtg gcaaaccttc gagagctata aagtcttttg tgcctggtcg attttttcgc
    24241 tatgggcatt tttcggctgg tacaccttaa caaacgtcga aaagacctgg tctttcgccg
    24301 cgctttgccc gctggggctg gcgttgctgg taggattttc aattcctgac agagtcatgg
    24361 aaggaaaaca tccgcaattt tttgtcgaga tgacacaaga atcacttcag ccaagccgct
    24421 atattcttac tgatagcgtc ggtgttgccg caggtctggc atggagcctg caacgcgatg
    24481 acatcatcat gtatcgccag acaggtgagt tgaaatacgg ccttaattat ccggatgcga
    24541 aagggagatt tgtcagcggt gatgagttcg caaactggct taatcaacat cgtcaggagg
    24601 ggattattac actcgtgctt tcggttgacc gcgatgaaga tatcaacagt ctcgccattc
    24661 cgcccgcaga tgccatcgat cgtcaggagc gtctggtgct gattcagtat cgtcccaaat
    24721 gatctggcta acattagtct ttgccagctt gcttagcgtt gccgggcagt tgtgtcagaa
    24781 acaggcaacc tgctttgtgg cgataaacaa acggcgcaaa catatcgtgc tgtggctggg
    24841 actggcgctg gcttgtcttg gtcttgccat ggtgctctgg ctgctggtct tgcagaacgt
    24901 accggtaggc attgcctacc cgatgttaag tctgaatttt gtctgggtga cgctggctgc
    24961 agtaaaactg tggcacgaac cggtatcgcc gcgtcactgg tgtggggtgg cgttcattat
    25021 tggtggcatt gtgatcctcg ggagtacggt gtaatgggcc tgatatgggg attattcagc
    25081 gtcattattg cctcagttgc gcaattaagc ctgggttttg cggcaagtca tctgccgccg
    25141 atgacgcact tatgggattt tatcgcggct ctgttagctt ttggcctgga tgcccggata
    25201 ctgttactcg ggctgctggg ttatctgctt tccgtttttt gttggtataa aacgctacat
    25261 aaacttgcgc taagcaaagc ctatgccttg ctcagtatga gttatgtgct ggtatggatt
    25321 gcttcaatgg tacttcccgg ctgggaaggg actttttcgc tgaaagcact actgggagta
    25381 gcctgtatta tgagcgggtt gatgctgatt ttcctgccca cgacaaaaca acgttactga
    25441 gttttccctg ccgctgtaca ctgccgttcc cactcatcgg ggctataact gcttgccgag
    25501 aggactttca ccgtatgcag cttttcacga ttaatacaat aaagcgcatt ctgcaaaggc
    25561 gagagtaaat cccccacttt cacggcgaag tcactcttca cttcggcgat catttttatc
    25621 gcgccgccag catcgcagag cataagtaca tgtctgttat cttgtttatt gcagcacgat
    25681 tttttgtgcc agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta
    25741 cagattaaca gataaactct ttatcaacgg caatggaatg gcagaaatga gaagtgaaac
    25801 ggagggttaa ttattgctga cgttgcaccc actcttttag cgcctgacgt gaaattttaa
    25861 taccgccgtt tttcagctcc ggcggtaaag tcagccagcg caccggttgt tgaaaacgag
    25921 ccagcttatc tttcacccat tcgccaagat caacggtttg ctggtcatac tccatcaccg
    25981 ctaccggccg atgaccaaac tccttgtcgg caacgggaac gataaacacc tgcaaaaccg
    26041 caggatgtgc agcaataaca cgctcgactt cttccggctg aataccctca ccgccactga
    26101 agaacagatt gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat
    26161 cgcgcgtagc gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt
    26221 aaccttctgc catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc
    26281 gacccggcag cggcaaacca acgtctgcca ggccgtcggc ttctttcgca cataccgtgg
    26341 aggcaaactc ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt
    26401 ccgtcaactc gaccgggata gccgcgccgc caagtaacac cgctttcagt gaaacggaac
    26461 tgcggttaac cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg
    26521 ccagcatttg ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc
    26581 agcgccacat aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat
    26641 cgtgatcgcc aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat
    26701 aagtatggac agcggctttc ggcaaaccgg tagaacccga ggtcaacgtc attgagcaca
    26761 gacgcgtcgg ctgccacgca gcggcatgta cgccttcaac cagctgaatg tgcagcgacg
    26821 ttaatgccgg aaacgtgttt tccccatccg gcaccagcgc aaattgcagc gtcagattgg
    26881 gcagcaattc ttcaagcaac ggttgcggca gctgagggtt cacgggcaac acccgcgccc
    26941 cgcattgcag taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca
    27001 tcacgccgct gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc
    27061 gagcacaaag ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc
    27121 cccgcacttg ccgccagtga cgccacggcc agtcagagaa gatcataaca actgctccag
    27181 tacatcagcg tccactaacg gcagtgggct ccccggccag cgacgaacct gctgcgcctg
    27241 catcagatcc agcgtgtcca gtcccggaat ggtgtctggc gttaaccagg cagcaatccg
    27301 cgccagttgc gttagaccta agctcgactc aatggaagaa ctgatcaccg ccgtcagccc
    27361 cagcgcatgc gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg
    27421 tttgataact accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag
    27481 gctttcatcc caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg
    27541 ggttttgcac ggctcttcga gaaacgcgat gcggtggcga taatcagggt taacatattt
    27601 ggcaaactgc tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag
    27661 atccggaatt gcctccagca acagattcac caccatgccg tcgcgcaccg cttcgtacaa
    27721 tcccactttg accttcgcca ctttctcgcc cggcatatcg gcaagtttga ggatcagatc
    27781 gtccggatcg ccattacaca gcggtgccgc acggtagttg gctgcttgcg gcaacgtatc
    27841 tgtcagttct gccaatgcac agcttacgcc aaaggccacg gaaggcatct gcggtagctc
    27901 gcaatcgcct gccagccagt tatttaccca ggcaagcagc acgctttgcg cctcttccca
    27961 ggtttcctgg ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccctc
    28021 acgcaggcaa acgtacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc
    28081 cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta
    28141 aatttgctga aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct
    28201 tccgtcatgt agaacagcat ggtggcgttg cccgccagct cctgcagccc cgcctgcccg
    28261 tcacagtcgg cgttcagcgc agctttcagg cagcgcagcg ccatcgggct gttttgcagc
    28321 atttcgcggc accaacggac ggtttctttt tccagatccg ccagcggtac cacggtgttg
    28381 acaaggccca tatccagcgc ctgttttgcg tcgtactgac ggcacaggaa ccagatttct
    28441 cgcgcttttt tctgcccgac gatacgagcc atgtaggaag cgccccagcc gccgtcgaag
    28501 gaaccgactt tcgggccagt ctgaccgaag atggcattat ctgctgcgat agtcaggtcg
    28561 cacatcatgt gcagaacgtg accaccgccg atggagtagc cagccaccat cgcgacaacc
    28621 ggtttcggac aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg
    28681 gaatcatctt tatagccgcc gtaatcacca cgcactttct ggtcaccacc ggagcagaac
    28741 gctttatcgc ctgcgccagt cagaatgatc acgccgatat tgtcgtcata acgcgcatct
    28801 gccagtgcct ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga
    28861 cgattaatgg tgatttttgc gataccgtcg gtggattttt cataacgaat gtcctcgaaa
    28921 ccttcggagc agtcgtgcca ttcaaccggt gcgtaaggca ttgcttcatc aggataaatc
    28981 atattgtgtc ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg
    29041 gattttcccg atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt
    29101 ccgccgccag ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg
    29161 tgcgggcgct aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca
    29221 tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat
    29281 cgtcattgag cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg
    29341 gttctgtgcg aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcacgttgtt
    29401 cagcattttg cagccccgga tgcccgcctt cgacaataac cccacaaagc cccgccagcc
    29461 cctggcaagc cgccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt
    29521 caaggatatt gtaactaacc aaggttttac gcagtaagtc ggtgacatca tcaaatccat
    29581 cgacgctaat cgccgccgaa ccaccatgac ctgggagatc aacatacaac cgtgagtagt
    29641 cggcaaacgc ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa
    29701 acaccagcca gggtaaacct ggttttccgt gttttgcctg cgcgtgcagg atcataaatg
    29761 gcttacctgc gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat
    29821 ttcaatcacc gtggtggttg gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg
    29881 ccagttttgc ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac
    29941 gttttgcggc atcagataga aacgctcacg ctcgctttgc ggcgttggca acagcgagaa
    30001 aatttgcccg ccgttgttgt tcaccacaat taataccagc ggcgcagaaa cctgacgcaa
    30061 taacgccagc gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg
    30121 tttgccgctt gcccgctgaa cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc
    30181 ccctcggttg ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg
    30241 taccaccagg ctgttaccaa caaacaattg cccctgttca ggcagatagt cgctgatacg
    30301 atgcgccagt tgcgcttcgc caaacgcatc acggcgggca ataaccgcct gcattgcctg
    30361 ttccgccagg cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc
    30421 cagccagtcg gcaatattgg caattaagcg acgtccgcga tggtgtgccg gatcaagtcg
    30481 cccttcaatg tcatcaacaa tccagtactc ttctggttca cagcttgcct gccattgcag
    30541 gagccgtttc cccgtcaggc agcttcccag ttgcaccaca atttgcgcct gctgcagctc
    30601 gctggtcgct ttggcattgc ctaaccaaag atcggcacac ggcagcggct gcccggtttg
    30661 tgacagcaca tcgccaatca gcggccagcc aagagtttgc gcccacaggg caactttttt
    30721 gccctcttcc gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa
    30781 ccagtcgcgc tgtttttcac tttccagacg aggcgcttca cgcagccacg gtttgtcgtc
    30841 ctgccaccag tcacccagac gctgttgcca gctaagcccg gtatcgtcca tttcgccata
    30901 cagcggttca gcaaacgggc agttgatatg gactcccccc acatgaagcg taccgagagc
    30961 gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga
    31021 aatactgtgc gtggggtgag agccgaacat tcccggctgg cgaattgcct gattcgcgcc
    31081 gcagtcaatt agctccggcg ggcgatcggc ggttaagaga atcagttttt ctccggttaa
    31141 cccggcttca atcagtgccg gatagagatt tgccaccgcc gtgccggagg tcacaatcac
    31201 cgccaccggc tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc
    31261 atcgaaatgg gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt
    31321 agaacgcgag cctggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag
    31381 aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa
    31441 tattgcggct aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg
    31501 cgactcatta ctattccatt tgtaataaag tacgcagcac tgccgctttg ttgtcgattt
    31561 cctgccactc ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca
    31621 cgacattgcc gctaattttt gctgagcgca gggaaacgca gaattcgctt tgttgtaatg
    31681 agagatagcc cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa
    31741 actgtcgcgc cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta
    31801 aacagatcac atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca
    31861 ctttacgcag acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta
    31921 atcgttggca gatatcttcc accaccagca tgttctcgcg ctggttttta tcatccgcca
    31981 tcagccactc tcctaactgc tgcgcctgct tatcatcagg atgatttgct actgttcccg
    32041 ccagcgcttc agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc
    32101 caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc
    32161 gacgactggc agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg
    32221 cccgagcgag caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga
    32281 ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta
    32341 acccaggcaa gggcttgata ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat
    32401 cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc
    32461 gtcgccattc caggcggggt aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc
    32521 cccaaatgcg taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca
    32581 acgacgtaaa acgggtaatc gcgcccaggg cggcagcttc ttcatcacca ttacgttgtt
    32641 gccagtagaa ttgcgggtac gtttgctgac tggccagcca gctcaaggca tcaaaagcgt
    32701 ctttgagagg gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg
    32761 acaaatggcg cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct
    32821 gttaaaagcc tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga
    32881 ggttttaacg gcgtgccagc aacagtccta gtaccagccc gacggccgcg cccacaccaa
    32941 ttccttgcca gggtttttcg tggacgtagt catcagcacg ataaacagcc tgcttcgccc
    33001 gatagtaata actgtctgaa gcctggctaa cccgtttttt cacatcatcc agcgcttttt
    33061 ctgcacgcgc tttcagctca acatatttct gatcggcggg atcgccagag gagcggagca
    33121 cctcttccag tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact
    33181 gattagacat tctcgttctc catttgcgta aaacctgtca ctaactatag acaagagttg
    33241 ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat
    33301 agacctccgt cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg
    33361 ccccgaggta aacaggctta tcaggccagt gatgcgtaca tgtttcgagt gttttactca
    33421 tcagctgctg ccccactttt tcaccacgca acgcttcact gacaatcacc cgacctataa
    33481 cgaccggctc aagatcatca tcacttttca gaatcctcgc atacgccacc agttcatcat
    33541 ttttccaacc gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat
    33601 aagggcaatt ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct
    33661 gggaaaccga taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt
    33721 agatgtatgc cttatactag ccggaatatt ttttgaaacg tgatgaactc atggaattaa
    33781 tttttttagg tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc
    33841 ttaacctgca acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc
    33901 agcatcagct actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc
    33961 accttcatgg cgatcatctt tttggtttac ccggcttgct gtgcagtcgt tctatgtcag
    34021 gcattatcca acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg
    34081 cgctgcggat tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg
    34141 gcgaaattct cgatgatggc ctgcgcaaag taaccgctta tccgctggaa cacccactgg
    34201 aatgttatgg ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat
    34261 taaaagctgc tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa
    34321 tcacgctgga agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag
    34381 gtaaagcgct cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta
    34441 aaggtgtcga tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca
    34501 atagtcgcgg ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg
    34561 gcaagctaat cattacccac gtcagctcgc gctatgatga caaaggttgt caacacctgt
    34621 tacgtgaatg caggtcaatt ttcccggcga ctgaactggc gaatgatttc accgtgttta
    34681 acgtttaact ctgcttatta ttaaaaacag ggtgaaactt gccctgttat agcaacccgc
    34741 gctacatata tccacccaca caggcatatt gagcgatttt tgatatttat tcatctcctt
    34801 acgcactata ttcagagcaa taagttgcct cttttcatct tgattcagca caacctttgc
    34861 aactaaaagg agcccatgat ggttacagtt gtcagcaatt attgtcaatt atctcaaacc
    34921 caactcagtc agacatttgc agaaaaattt actgtgaccg aggaattact gcagtcttta
    34981 aaaaaaacag cgttatccgg agatgaagaa agtattgagt tactgcataa tattgcgtta
    35041 ggttatgatg aatttggaaa aaaagctgaa gatattcttt accatattgt tagaaaccca
    35101 acaaatgaga ccctatcgat tatccgactt attaaaaatt cctgtttaaa attatataat
    35161 ttggcacata ccgcaaccaa ctcccacctt aaaccaactg gtccggataa ttcagatgtt
    35221 ctcctgttca aaaagctatt ctccccttcg aaattaatga caattatcgg taatgaaatt
    35281 cctcttatat ctgaaaaaca gtcgctttca aaggtgcttt taaatgatga gaataatgaa
    35341 ctgagtgatg gtacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagct
    35401 tgctatcttc agaagatcgc cgctaatgca aaaaatactc aagtcaatta tcctactggt
    35461 ctctacgtcc cctactccac cagaactcac ctggaagacg ctctcaatga aaatattaag
    35521 agcgatccat catggccgaa tgaagtccag ttattcccca taaatactgg cggacactgg
    35581 atattagttt cgctacagaa aatagtaaat aaaaaaataa taaactacaa ataaaatgcg
    35641 tcatattcaa ctcattgcgt gcactaggcc atgataaaga aaattcactt aagcgtgtca
    35701 ttaatagttt taattctgaa ctcatgggag aaatgtcgaa taataatata aaagttcatt
    35761 taaatgaatc agagataata tttttacatg ccgatcttca gcaatactta agccaaagtt
    35821 gcggtgcgtt tgtgtgcatg gcagcccagg aagtgattga acaaagggaa agcaattctg
    35881 acagcgcccc ctatacatta ttaaaaaact atgctgacag atttaaaaaa tattcagcag
    35941 aagagcagta cgaaattgat tttcaacatc gactggtaaa cagaaattgt tatttagata
    36001 aatatggcga tgcacgaatc aatgcttctt atacacaatt agaaataaaa cactcacaac
    36061 ccaaaaatag agcatccggc aaaagagtga gttaacatca ttgttaatca attcgaccag
    36121 tcagtaaata tgctgactgg tcgaacataa tttgctaaaa agtcatcact gactgatgtc
    36181 agtcccacca tctgccagtt caatctacgg gtatttccag cgtatcgcgg cacgcgattg
    36241 ctggtctgtt gatgtagtgg attgtagttg ctgacttttc ttaccctcag atttctgccc
    36301 cacttgtcaa ttcaatctgc caataaataa cataattata tttttattcc tcacagaaat
    36361 tcctttttaa aaatagcgaa cccactgtaa aaaacacttt ttgtatacct ctaccaagcg
    36421 taataaaata aacgctcata attcattata aataataatg aaataacgcc ttttattgat
    36481 aatttacata tgaaaaaaat tttccttttg ttatattatt tctgttttca ctccctctga
    36541 ttatttttac caaccctggg tcactgcatt atcgccaacg ccccgacacg taagtcctga
    36601 gcaattagaa aaaaca
//
LOCUS       DADLSE010000024        36323 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLSE010000024 DADLSE010000000
VERSION     DADLSE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.024"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(29..208)
                     /locus_tag="JN692_001217"
     CDS             complement(29..208)
                     /locus_tag="JN692_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7263576.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN692_001218"
     CDS             complement(292..1176)
                     /gene="yhdJ"
                     /locus_tag="JN692_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7263577.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN692_001219"
     CDS             complement(1262..1558)
                     /gene="fis"
                     /locus_tag="JN692_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7263578.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN692_001220"
     CDS             complement(1584..2549)
                     /gene="dusB"
                     /locus_tag="JN692_001220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7263579.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN692_001221"
     CDS             complement(2878..3759)
                     /gene="prmA"
                     /locus_tag="JN692_001221"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7263580.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLYDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRNNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN692_001222"
     CDS             complement(3771..5222)
                     /gene="panF"
                     /locus_tag="JN692_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7263581.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5212..5454)
                     /locus_tag="JN692_001223"
     CDS             complement(5212..5454)
                     /locus_tag="JN692_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7263582.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN692_001224"
     CDS             complement(5563..6912)
                     /gene="accC"
                     /locus_tag="JN692_001224"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7263583.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN692_001225"
                     /gene_synonym="fabE"
     CDS             complement(6923..7393)
                     /gene="accB"
                     /locus_tag="JN692_001225"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7263584.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8371..9345)
                     /locus_tag="JN692_001226"
     CDS             complement(8371..9345)
                     /locus_tag="JN692_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7263585.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9497..11437
                     /gene="csrD"
                     /locus_tag="JN692_001227"
     CDS             9497..11437
                     /gene="csrD"
                     /locus_tag="JN692_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7263586.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSIPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNARGQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11742..12785
                     /locus_tag="JN692_001228"
     CDS             11742..12785
                     /locus_tag="JN692_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7263587.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12851..13954
                     /gene="mreC"
                     /locus_tag="JN692_001229"
     CDS             12851..13954
                     /gene="mreC"
                     /locus_tag="JN692_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7263588.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13954..14442
                     /gene="mreD"
                     /locus_tag="JN692_001230"
     CDS             13954..14442
                     /gene="mreD"
                     /locus_tag="JN692_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7263589.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14451..15044
                     /gene="yhdE"
                     /locus_tag="JN692_001231"
     CDS             14451..15044
                     /gene="yhdE"
                     /locus_tag="JN692_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7263590.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15034..16503
                     /gene="rng"
                     /locus_tag="JN692_001232"
     CDS             15034..16503
                     /gene="rng"
                     /locus_tag="JN692_001232"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7263591.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16571..20371
                     /locus_tag="JN692_001233"
     CDS             16571..20371
                     /locus_tag="JN692_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7263592.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20527..21972
                     /gene="tldD"
                     /locus_tag="JN692_001234"
     CDS             20527..21972
                     /gene="tldD"
                     /locus_tag="JN692_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7263593.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN692_001235"
     CDS             complement(22100..23029)
                     /gene="aaeR"
                     /locus_tag="JN692_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7263594.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23212..23415
                     /locus_tag="JN692_001236"
     CDS             23212..23415
                     /locus_tag="JN692_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7263595.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23423..24355
                     /gene="aaeA"
                     /locus_tag="JN692_001237"
     CDS             23423..24355
                     /gene="aaeA"
                     /locus_tag="JN692_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7263596.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24361..26328
                     /gene="aaeB"
                     /locus_tag="JN692_001238"
     CDS             24361..26328
                     /gene="aaeB"
                     /locus_tag="JN692_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7263597.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPRDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26420..26692
                     /locus_tag="JN692_001239"
     CDS             26420..26692
                     /locus_tag="JN692_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263598.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN692_001240"
     CDS             complement(26748..27011)
                     /gene="yhcN"
                     /locus_tag="JN692_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7263599.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN692_001241"
     CDS             complement(27376..27846)
                     /gene="argR"
                     /locus_tag="JN692_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7263600.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28281..29219
                     /gene="mdh"
                     /locus_tag="JN692_001242"
     CDS             28281..29219
                     /gene="mdh"
                     /locus_tag="JN692_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7263601.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN692_001243"
     CDS             complement(29281..30348)
                     /gene="degS"
                     /locus_tag="JN692_001243"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7263602.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN692_001244"
     CDS             complement(30438..31805)
                     /gene="degQ"
                     /locus_tag="JN692_001244"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7263603.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31959..32357)
                     /locus_tag="JN692_001245"
     CDS             complement(31959..32357)
                     /locus_tag="JN692_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7263604.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32551..33678
                     /locus_tag="JN692_001246"
     CDS             32551..33678
                     /locus_tag="JN692_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7263605.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33897..34325
                     /gene="rplM"
                     /locus_tag="JN692_001247"
     CDS             33897..34325
                     /gene="rplM"
                     /locus_tag="JN692_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7263606.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34341..34733
                     /gene="rpsI"
                     /locus_tag="JN692_001248"
     CDS             34341..34733
                     /gene="rpsI"
                     /locus_tag="JN692_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7263607.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35136..35774
                     /gene="sspA"
                     /locus_tag="JN692_001249"
     CDS             35136..35774
                     /gene="sspA"
                     /locus_tag="JN692_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7263608.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35780..36277
                     /gene="sspB"
                     /locus_tag="JN692_001250"
     CDS             35780..36277
                     /gene="sspB"
                     /locus_tag="JN692_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7263609.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
BASE COUNT         8739 a         9055 c         9593 g         8936 t
ORIGIN      
        1 aaacgaatga attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac
       61 agttaaccag ttttagtgga ttaactgagt tcatattacg gcatttgtct gtttctgttg
      121 tcagcagttc aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa
      181 cgacgagcca ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg
      241 gcaggaaata tacacgaaat gaataatctg aaaaccaggc ttacacttcc tttactttgc
      301 aatgagatcg gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct
      361 ttttttcact ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag
      421 ccccattttg atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat
      481 ggctacggca ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt
      541 ggaagaggcg agaataatgc gtttcagtaa ggctttcggt ttttgcgtcg ggtggttctc
      601 atattcatcc atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg
      661 atgattgtat ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt
      721 tttggcttct accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat
      781 catcaggatg ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc
      841 atatgaccag acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa
      901 gggcatgttt tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg
      961 gcactctgca atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat
     1021 cagaccatca aaatttttac cgatgttata cggtgggtcg gcaaagagca gatcgacgct
     1081 ttcagtaggt agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc
     1141 attaccaaac cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat
     1201 aaaaaaggcg cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga
     1261 attagttcat gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca
     1321 tcagcgcagc acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct
     1381 gttctacttc agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag
     1441 caaaatagtt cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat
     1501 cctgagagtt aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag
     1561 ttctgtcagc tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc
     1621 agctgttcgc tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg
     1681 tgttcctgga gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca
     1741 taaaagtcat gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc
     1801 agtgggggca gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt
     1861 cttccctgag ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct
     1921 ctggctttaa gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc
     1981 ttaactgccc gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg
     2041 ccatgaatgg tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag
     2101 ttacggtgtt ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca
     2161 ttgacgacct cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct
     2221 gcgagcttgc gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca
     2281 ccgctttcca cgttaatacg tgctgcatct gccatttctt tcggagcgct accagcaatt
     2341 tgcacggtgc gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt
     2401 tcccaaacct gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg
     2461 tagcacaacg tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga
     2521 tttctgagct gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa
     2581 ggcggcgtat attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct
     2641 gagatggatc aaagaattgc atttaaaatg agcgtggtgc gataattact cataaaaatc
     2701 atcatattag aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat
     2761 ttttctttat gaatgaaaat tttggcacgc aaatttgcgt aaataatcgg caaatttacg
     2821 tgccttttgt gagcttgctc gcacttcgcc ccgcgtcacc ctacgccgat gcgaaggtta
     2881 attcttacga ccggtaatac ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa
     2941 gctatcggca taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc
     3001 cagcaaaccg cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc
     3061 tgcaaggatg ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa
     3121 gtagagttcc agacggtcag aaacgccatt acgttcggcg ttattgcggc tggcctgaat
     3181 cgcctgcgga tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat
     3241 cgccagaatg ccggaaccac agccaaagtc gatgactgtt ttaccggtta aatcgaggct
     3301 gtcgagccat tgcaggcaca gagaggtggt tggatgggta cccgtaccaa acgccagccc
     3361 tggatctaac atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat
     3421 ccacagtcgt tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc
     3481 tttatcttct agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc
     3541 cagaatcgcc accacgtcgt tcatatcggt ttcagcgtcg tacagaccaa tcacatcggt
     3601 gtcgccccac aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc
     3661 ctgaaaagtg atagaaacgg cacccgcttc catcagcgca tcgctaagat cttccgcgtt
     3721 cgcgccggtg gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca
     3781 gtagtcaaaa cggtagcttg tgggacggat gtaccgaaac ggtttccgac caggaaagcc
     3841 agcaaactta gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc
     3901 gtcgcgagta cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg
     3961 ttggcgcgtt cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca
     4021 ccgaaggcca gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc
     4081 agcaacgcgc cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt
     4141 tgcatttgat ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgc
     4201 agcagttggg cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg
     4261 gcagcaaaag gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg
     4321 agatcgggga tcactgcacg acctaacgct ccggccaggt gcataccgaa catcaggatt
     4381 gcgactacaa tcgtaccgat gatgatcccc cggtgtactg ctttgctgtc tttataagag
     4441 atacagcgca ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac
     4501 gacgtcataa aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga
     4561 tcgatggttt gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca
     4621 ataagcagca caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg
     4681 ctggcgcgaa agccaccaaa ggcggtatat aacgcaatgc tgataccaaa aatcagcagc
     4741 ccggtttcat aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc
     4801 acggtcatcg caccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga
     4861 cgactctggt agcgggcaaa cagcatatcg ttcagcgtca ctgcattgta gcggcgcgca
     4921 agaatcgcaa acttcttgcc gagaataccg agtgaaagcc agacagcagg aagctgaatc
     4981 atcgccagca atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac
     5041 gaactggcac tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag
     5101 cggctgccga ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa
     5161 accgagatac cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc
     5221 atcgtcatcc tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc
     5281 aataaacagt agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa
     5341 accgggggca acgccaggta agtaagcgtc tactaaccaa actgccagat acaaaagggt
     5401 cagccccagc gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg
     5461 tccctgatgg gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat
     5521 aaaaaaggcc ggaaaatccg gccttttgac gcttttgcag tcttattttt cctgaagacc
     5581 gagttttttc tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat
     5641 gatgcggatc tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc
     5701 attcttcatg cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat
     5761 cattgagtca tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac
     5821 gccaaaaccg ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt
     5881 cggatcttcg gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg
     5941 cttgatcgac agcggttgac cggcagcgat acgcagctgt tctttgatca ggtcaacgcc
     6001 ggtgatcatt tctgtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata
     6061 gaactcgccg ttttcgaaca ggaactcgaa agtacctgca ccgcgatagc cgatatcaac
     6121 acacgcttta gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc
     6181 tggtgcttct tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag
     6241 atagatagcg ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc
     6301 caggtatttc tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt
     6361 catggagatg gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccgcc
     6421 gccgccgccg gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcgcggtt
     6481 tttatccata tcgtcgccca gcgggccgtc agaaccaggt acgcaaggaa cgcccgcttt
     6541 tttcatcgcc gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg
     6601 gccgatgaag ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag
     6661 gaagccgtaa cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc
     6721 cgggatgttc agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc
     6781 cagtaatacg tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc
     6841 cagttcttta caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat
     6901 tttatccagc atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg
     6961 gttgtccact ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt
     7021 tcatcatttt catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca
     7081 cttcgatgaa cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg
     7141 aacgtacgat gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg
     7201 ccggagcggc tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt
     7261 gcatcacagg gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt
     7321 cagaaatttc cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct
     7381 tacgaatatc catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc
     7441 accgccgtct gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg
     7501 ccgggacgcc ggtctatttt gccccagagt gtcaatgtta gacttgacgg acattgtgca
     7561 gcgatctgct gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc
     7621 ttttccgcag cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt
     7681 agcaattggc agctaaatac tggtcttatc agggaagata atcaacagct aacatgtaaa
     7741 taaccttcaa caccgtgtaa tttgtaacaa gccgcacaat tcacgaaatt agcgccatca
     7801 tcgacggaac ttcttataac gtaaggtgaa aagctgaaaa gccagccctg cgaagatgag
     7861 gggctgcaat gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac
     7921 aagatagacg acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg
     7981 gatagatgtg aatgctaaag cacgcaaaat caaccaactg acaattctca gcattaaata
     8041 ggttcgagtg gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa
     8101 atcagtaaca ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac
     8161 ggaggcgtat caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt
     8221 cgaaatcatg actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac
     8281 aaaagctgtc cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag
     8341 ttccctctct tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg
     8401 gatctggtta ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt
     8461 tgccgcctgg gtatagaatg attccggtaa atcggcgacc agtcgctgcc aagcttgtgc
     8521 gcggcgttct ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat
     8581 aaatggcatg accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca
     8641 gccgccgtaa ttcatttgcg ctagcacttt tgccagcact ttgtcgccaa cagtgtcaat
     8701 tgccccagcc cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag
     8761 aatacggctg gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc
     8821 aacgacctga taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact
     8881 ggcacccgtc acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat
     8941 cacacacagc atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag
     9001 cccttgcggc atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc
     9061 ccagtggttt tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg
     9121 cggatcttcg ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt
     9181 acggatgatt tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca
     9241 gtgaacatcg accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtactga
     9301 tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt
     9361 gcatgatgga ttggaaaata atttctgaag actatactcg ctaatggaaa agcaaagccg
     9421 atgaagcgca ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat
     9481 cggagttaac acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac
     9541 cgggttaaca atttttgtga ctttgctggg ctgttcgcta agtttctaca acgccattca
     9601 gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc
     9661 gaatgacttc agcgcattaa ggccacaaat taccgaatta atggtgtcag cagatatcgt
     9721 tcgtgtagac ctgctgcatg gtgataaaca ggtttatacc ctggccagaa atggtagtta
     9781 tcgtccggtt ggcaccagcg atctgtttcg tgaactgagc attccgttga taaagcatcc
     9841 ggggatgtcg ttgcgtctgg tttatcagga tccgatgggc aactatttcc attcgttgat
     9901 gaccaccgcg ccgctcacgg gggcgattgg ctttatcatt cttatgctct ttctggcggt
     9961 acgctggtta caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat
    10021 cttaaacggc gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac
    10081 cagcagtgcg ctggatacgc tgcttcgtga aattcagaac gcacgcggac aacacagccg
    10141 tcttgatacg ctgatccgct cttatgccgc acaggacgtg aaaaccggcc tcaataaccg
    10201 actctttttc gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca
    10261 cgggatcgtg atgatgattc gtctgccgga tttcaatatg ttgagcgata cctgggggca
    10321 cagccaggtt gaagaacagt tcttcactct gacgaatctg ctgtcgacat ttatgatgcg
    10381 ctaccctggc gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca
    10441 ccggacgtta aaagaggcag agagcatagc cagtcagtta atcaaagccg tcgatacctt
    10501 gccgaacaat aaaatgctcg atcgcgacga tatgatccac attggtatct gtgcctggcg
    10561 tagtggtcag gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg
    10621 attgcagggt ggcaatagct gggctattta tgatgactcg ttgcctgaaa aaggacgcgg
    10681 taatgttcgc tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta
    10741 tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat
    10801 cttcgatggt aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg
    10861 cttatcggaa gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg
    10921 gccagaggaa aatctggcga ttcaggttac cgttgagtcg ctgattcgtc cgcgttttca
    10981 gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat
    11041 tgaacttgca gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt
    11101 agtgaatgct ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac
    11161 cagttggatc aaagaactta atgttgagtt actcaagctc catccggggc tggtcaggaa
    11221 cattgagaag cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg
    11281 gaccagcacc cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat
    11341 tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac
    11401 taacgtgaaa aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac
    11461 gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca
    11521 gcacatatgc agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc
    11581 attaagcctt tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt
    11641 tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg
    11701 gattttcttt tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg
    11761 gcatgttttc caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa
    11821 aaggacaagg catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg
    11881 gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc
    11941 cgggcaatat tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga
    12001 ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa
    12061 gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc
    12121 gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg
    12181 ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg
    12241 gtggtggtac cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg
    12301 tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg
    12361 gttctctgat cggtgaagca accgcagaac gtatcaagca cgaaatcggt tcggcttacc
    12421 cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac
    12481 gcggttttac cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta
    12541 ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct
    12601 ccgagcgcgg catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt
    12661 taatggaaga aaccggcatt ccagtcgttg ttgctgaaga cccgctgacc tgtgtggcgc
    12721 gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag
    12781 agtaatcgga tgcaggcagg ggaagcgtct gtttaccctg cctggtctga tacgagaata
    12841 cgcataactt atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct
    12901 ggcggtgctg gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca
    12961 aatccgtact tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg
    13021 tgaattgctg gatggcatat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa
    13081 ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata
    13141 caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca
    13201 gaaaatggtg actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat
    13261 cgataaaggt agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt
    13321 tgttggtcag gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc
    13381 gacccacgcg ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa
    13441 cggttgtacg gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg
    13501 tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt
    13561 tgtctcttcc gtaaaactcg atacccagcg cgcttatact gtgatccagg cgcgtccgac
    13621 tgcaggactg caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc
    13681 taacccgatg acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat
    13741 gccgcaggta ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg
    13801 gatcgctcag ccgactccgc agcaaccggc gacaggaaat gcagctactg cgcctgttgc
    13861 gccgacacag cccgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc
    13921 tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgc
    13981 tgggtaatct ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat
    14041 aacctgattg ttttccggcc aaactgggtg ttacttatct tgttgtattg gatcctggcc
    14101 ttgccccatc gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc
    14161 agcggctcga tgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg
    14221 ctgaaatacc agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg
    14281 ctttcgctgg tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct
    14341 ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc
    14401 ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc
    14461 tgtatttagc ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggcgtgacct
    14521 ttgaacgtat tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt
    14581 atgttgtgcg tctggcacgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc
    14641 tcccggtgct gggtgcggat attatcgtta tcctgaacgg agaagtgctc gagaaaccgc
    14701 gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga
    14761 tgacggcagt ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg
    14821 tgactttcag aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt
    14881 tagataaagc aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa
    14941 atggcagcta tcacgccgta gtcggcttac cgctggttga aacgtatgaa ttattaagta
    15001 attttaacgc actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta
    15061 acgccttcgg aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt
    15121 gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta
    15181 cttccgggta tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat
    15241 gcatccgaca tcatgccgca taccgaatgt gtggcgggtg aagaacaaaa gcaattcacg
    15301 gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa
    15361 gatccgcttg gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat
    15421 ctggtattta tgccaggggc ttctcacgtt ggggtttccc aacgaattga aagcgaatca
    15481 gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc
    15541 atccgcaccg cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg
    15601 aaacgcgtct ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg
    15661 tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt ttgccgacgc cgaactggac
    15721 cgcattcgcg ttgactcacg cctgacttac gaagcattgc tggagtttac ctcggagtac
    15781 attcccgaga tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc
    15841 tttgatgtcg aaaacgaaat ccagcgagcg ttggaacgca aagtagaact gaaatccggt
    15901 ggttatctga ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg
    15961 tttgtcggtc atcgcaatct ggacgacact attttcaata ccaatattga agcgacgcag
    16021 gctatcgctc gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc
    16081 gatatgaata atgaagatca ccgccgccgc gtgctgcatt cgctggagca ggcgttgagc
    16141 aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg
    16201 cgtaaacgca cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac
    16261 ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt
    16321 cgtgtccacc atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc
    16381 gaagccttga aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa
    16441 caggttaaag tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg
    16501 taaacagatg ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa
    16561 ggagtgacgg gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt
    16621 gatcgctgcc ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg
    16681 tccggaaatc ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct
    16741 ctcagccagc tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact
    16801 aaaagatggc ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag
    16861 cctgttacat atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac
    16921 caacactcct atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga
    16981 tctgtttctt cgtcaatttg accatttcga tctccgcgac agtgaagtca gttttctgac
    17041 gccatccggt cagcgcgccg agctggcgat cccacaactc acctggctga acgatccacg
    17101 tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat
    17161 gcaggtgcgc atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct
    17221 acaggcggat gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc
    17281 tctggaaacg gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac
    17341 cggcggtgac gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca
    17401 tacgctgtcg gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt
    17461 ctctattccc gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac
    17521 gctggcctgg ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact
    17581 ccggattcgc gctagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc
    17641 gaaactttca cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa
    17701 cactctggcg ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg
    17761 gagcgatctg gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac
    17821 gctttccggc agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc
    17881 ttacgaaacg gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg
    17941 gctgaacaat gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc
    18001 cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg
    18061 tattctggct ggcatcagta ccgatgatgg ttcacaagcc tggcgctatt tcccggaaaa
    18121 cttgatgggt aaagacctgg ttgattattt aagtggtgcg attcagggcg gtgaagcgga
    18181 taacgcgacg ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg
    18241 tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc
    18301 tgcattaact aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac
    18361 cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta
    18421 ctcaaaagaa aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc
    18481 ttactttgat gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct
    18541 cgacggcgat gtgaatgctc gcttacatct tgatatcccg ctgaacggcg aactggtaac
    18601 cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac
    18661 cctgaaaaat ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact
    18721 gacagcaagc tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa
    18781 agcctaccag gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct
    18841 gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat
    18901 tgatctgcct tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa
    18961 tgtgagtagt cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa
    19021 cgttaaggtt gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa
    19081 ccatttcaat agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc
    19141 ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac ttaatatgcc
    19201 gccgatgaat ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg
    19261 tggtgcagcg agtttcccac aacacataac gttacgtacg cctatgttgt cactgggaaa
    19321 tcagcaatgg aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga
    19381 ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc
    19441 gaatatcaaa tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc
    19501 gtcatcacct ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat
    19561 acgatgcaca gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac
    19621 catttctggc gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct
    19681 tactgccgat ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa
    19741 actgcgtggg cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg
    19801 ccagtcgtca tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga
    19861 tgaagcgacg ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat
    19921 caataccgga catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa
    19981 gctgcgtttt gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag
    20041 caccgcgtgg attaaagacg gcgttatgca caccgacgac acgctggtgg atggcctgga
    20101 ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc
    20161 ggttgtcgca ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc
    20221 cattgttggc gcggcagtgt ttgccgccag taaagtgctg gggccgctgt ggagcaaagt
    20281 ctccattttg cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt
    20341 gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc
    20401 tcatatgata atcgttgcca aaggccaacg agccggaaca taactgtagg ccggataggg
    20461 cgttcacgcc gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca
    20521 aaaacgatga gtctgaacct ggtaagtgaa caattgctag cggcgaacgg cctgaaacat
    20581 caggacttgt tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc
    20641 tattttcagt cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt
    20701 tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt
    20761 gcttacgctg accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc
    20821 atcgtccgtg atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg
    20881 ttgtatacct cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg
    20941 cgtcgcgtcg ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc
    21001 ctcagtggtg tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat
    21061 gtccgtccac tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa
    21121 cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac
    21181 ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt
    21241 tctgccgttg ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc
    21301 gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgtggcact
    21361 tcagtattta gtggacaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat
    21421 gacggcacga tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc
    21481 cagtacaacg tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac
    21541 gcgcgtttga tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg
    21601 cccatgccgc gtatgaccaa cacctatatg ctgccgggta aatcgacccc gcaagaaatt
    21661 attgaatccg ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc
    21721 acctccggca aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta
    21781 acgaagccgg tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt
    21841 tcgatggttg gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg
    21901 caaagtttgc cggttggcgt gggccagcca acgttgaaag tcgataacct gactgttggc
    21961 ggtactgcgt aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa
    22021 cggtcccctc gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa
    22081 cgtaacagta caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt
    22141 taccgacctc aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca
    22201 gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca
    22261 gcaggatctc cagctcccca cgattgatct cgttgatcac ccacatcagc ggcacgtagg
    22321 cgatcccggc acccgccgtt agccagcgca ccagcgtcat cggatcatta gtcacaaatc
    22381 tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc aatcagttca aattcattgt
    22441 cgggccgcac gctgtattca agccatgaat ggctacttaa atcggcgggt ttttccggta
    22501 tgccgtattg tgtgagatag cttttcgcgg cgcacaccac cattggcatc gcgccaagac
    22561 ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt
    22621 cggcaatcag gtcgggggct ggaattccgg taaccagatt ggcgctcaaa cctgggtatt
    22681 ctttcagcat tttggctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc
    22741 caatgcgtag cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct
    22801 gcacttcatg aagcatacga cggcagccct ggtagtaaat tctaccggct tcggtcaggc
    22861 caatgctgcg tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca
    22921 ccgtctgact gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc
    22981 caaattcaac tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg
    23041 acttaaaagt gatttagatc acataatata gataacagca taacagttac gctaatatat
    23101 taaatatcaa tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg
    23161 acagatgctg aaaataataa cgcctgctct ctctatacaa ccaaggtcaa catgagtctg
    23221 tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga tattttttga attgctttta
    23281 tcactggcga ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt
    23341 gtctggcatc cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg
    23401 cgactgttcg tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat
    23461 cacggtcgta ttagtcattc tggccttcat cgcaattttt aatgcctggg tctattacac
    23521 cgaatccccc tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga
    23581 cgtttctgga ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca
    23641 gatactgttc accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga
    23701 tgttgcttat tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg
    23761 tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag gctaacaacg tactacaaac
    23821 ggttctgcat cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga
    23881 acgcacggtg atccgagcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg
    23941 tgagtttatt actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt
    24001 actggcctat atggaagaaa ctaagctgga aggggtgcgt ccggggtatc gtgcagagat
    24061 cacgccgctt ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgctggggt
    24121 caccaacgcc agcagcacgc gtgacgacaa agggatggcg accatcgact ctaaccttga
    24181 atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa
    24241 catctggcct gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga
    24301 aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg
    24361 atgggtattt tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc
    24421 attgtactgg cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta
    24481 ctgacagcgg cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct
    24541 ggcgctattc gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc
    24601 ggactggtga tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt
    24661 atctgggccg gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg
    24721 tgggggctgg ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg
    24781 cttacgccgc agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg
    24841 attatggcgg atttgctctt ttctccgcga tcgatcaaac aagaagtaga tcgagagctg
    24901 gaaagtttgc tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt
    24961 gaagttgtcg ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg
    25021 cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg
    25081 atcaatacgc tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat
    25141 acgcgcccgg aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc
    25201 gcgcaggacg tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa
    25261 cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc
    25321 aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc
    25381 gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga
    25441 accacacttt cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc
    25501 agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca
    25561 cgcatggtgg cgatcgactt tatctatggg acgctggccg cgctgccgtt agggctgctc
    25621 tactttttgg tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg
    25681 gcagtgctgg gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg
    25741 gcactggcca gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt
    25801 cagtttctcg acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt
    25861 ttgctggtgc gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt
    25921 tctgccgctg tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg
    25981 gcactttatc agcagctgtt tttgctgatg aataagttcc caagggattt gccgaaattt
    26041 cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt
    26101 aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct
    26161 gcccgtagcg atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc
    26221 taccaggaaa agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg
    26281 ctggcgggga tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac
    26341 gccaaaagcg tcggtttttt catggctata cttagcgatg cacggcagaa ctcgccgcga
    26401 aacgtgacgg tggcaacaga tgaatattta tgcctttgat tttgatgaga ttgagagtca
    26461 ggaggatttt tatcgtgact ttagccaaac ctttggtctg gctaaagata aggtacgcga
    26521 tctcgactca ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga
    26581 atttgttcat ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga
    26641 tgaggcagag gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa
    26701 aaagcccccg aaccgggggc aatatcgtcg gacaagacga tgagggttta tttgtacagt
    26761 tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag taatctggta ggccgttgcg
    26821 cctttctctt ccgctttttt gttcagcatt tcacgtatat ccattggcga agacgccaca
    26881 ccacttacgg atacggtccc gattgcttca cgattttgtg cttgtgcagc atcaatggag
    26941 tcggcagcga atgcaccgaa agagagaaca gaaagtacgc ttaatgcagc tacagtggtt
    27001 ttgattttca tgatttttac ctcgacataa tcttttagct gggcctttgt ttcgtgaccc
    27061 atatcacaaa atcaagtata cactaattat tgaactaatt aataccacgc taactgtttc
    27121 agttgatact aagtgttaaa aatgtgaatt ttaataacaa aaaagtataa aaaacacggt
    27181 tatttattat taattatagt attttcaatt agataatgaa ttttgcataa tgtgcttttt
    27241 ttaccgtttg tattcatttt gtgaatgatg tgtcgcggta aaacgcacta ttcgttaaat
    27301 gtatcgcagg agaaagaagg ggaatgagag ggataagcaa cattttcccc gccgccagaa
    27361 gcgacggggc agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc
    27421 tttgacggtg aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc
    27481 cagaatacct tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc
    27541 agggctggta tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt
    27601 caatggactg gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat
    27661 tttggcattg cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg
    27721 attaatattg tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa
    27781 tttctcttct ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct
    27841 tcgcataagt cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt
    27901 tatgcaaaca gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat
    27961 caaatttcgt tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa
    28021 attaattgtt atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct
    28081 gttggaatgt tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta
    28141 cggcaattaa atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat
    28201 tgtaaacggc aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc
    28261 aatataataa ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc
    28321 aggcgcttgc actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg
    28381 atatcgctcc agtgactccc ggtgtggccg tcgatctgag ccatatccct actgctgtga
    28441 aaatcaaagg tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc
    28501 ttatctctgc aggcgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta
    28561 acgccggcat cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca
    28621 ttggtattat cactaacccg gttaacacca cagttgcaat tgctgctgaa gtgctgaaaa
    28681 aagccggtgt ttatgacaaa aacaaactgt tcggcgttac cacgctggat atcattcgtt
    28741 ccaacacctt tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta
    28801 ttggcggtca ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt
    28861 ttaccgagca ggaagtggct gatctgacca aacgtatcca gaacgcgggt actgaggtgg
    28921 ttgaagcgaa agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt
    28981 ttggtctgtc tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg
    29041 ttgaaggcga cggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg
    29101 gcgtggaaga gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag
    29161 gtatgctgga tacgctcaag aaagatatcg ccctgggcga agagttcgtt aataagtaat
    29221 tgattagcga ataataaaaa accggagcac agactccggt ttttgttttg agcactcgac
    29281 ttaattggtt gccggatatt cctgaatggt gacctgcagc gttaactgct tatcatcacg
    29341 catcactaca accgggatca ccgaaccagg gcgaatttcc gccacctgat ccatcgtctc
    29401 cagagcagag atggccggtt tgttatccac cgaaataatc agatcgttga cctgaatacc
    29461 cgcattcgcc gctgggccgt caggtgacac ttcattaacc acgatccctt gcagttgatc
    29521 tataccaccg ctctgcgcgt gcaatggtgc gatctcgcgt ccgccgatac caatgtagcc
    29581 gcggatcacg cgaccatcgc ggatcagctt atccataatt ttggttgcta actggaaagg
    29641 aatcgcaaag ccgatacctt ccggcgtttc gccatcgtta ctcttatcaa acgacagcgt
    29701 gttaatgccc atcagttctc ccagcgagtt caccagcgcg ccgccagagt taccgtggtt
    29761 aatggaagca tcggtttgta ggaagttttg ccgcccggtc gggttcagac cgattcgacc
    29821 cgtggcacta ataatccctt gggtaatggt ctgcccgagg ttgtacgggt taccgatcgc
    29881 cagtactacg tcgccaatgt gcggtacgcg acgtgcatta attggaatgg taggtaaacc
    29941 gccagtggca ttaattttaa gtaccgccag atcggttaga gagtcagatc ccaccagcaa
    30001 tgcttcaaat acacgtccat cctgtaaggc gacgatgatc tgatcggcgt cgttgatgac
    30061 gtgtttattg gtgatgatat aaccgcgttg atccatgatt acaccggatc ccagggtgcg
    30121 gatctcaagc tggttgtgag agttggtgtt caaaccacgg ttgtaaacgt taaccaccgc
    30181 tggcgcggcg cggcgaaccg ccagattata gctggcaggc gtctcatcgg tactgtcaaa
    30241 ttgcggagtg gaaagcgggt taaggctgcg cagcgaaggc atggcaacca gcagaatagc
    30301 gccgacaatt aatccaatcg caacggaacg taagagcttc acaaacatga tggaggcgtc
    30361 attaaaaaag ggaacggcag cagcatacca cgagttaacc ggacatcaca cgtcagcctg
    30421 atgcccggtt tacgacatta acgcatcagc agatagatgc tttcattgcc gcgtacaatt
    30481 tgcagggcga tgatggccgg ttttgccgcc agcactttac gcatttcagc aatcgagttc
    30541 acccgatcgc ggttgacgcc aatgatcaca tcgtcttttt gcaagccagc ctgagcagct
    30601 gggcttcctt tgacaacttc atctatctta atacctttgc cgccatcttt tagctgacca
    30661 tcgctcaacg ttgcaccttc cagcgctggc gtgatcattt cagcgctggc cgacgaagag
    30721 gtgctggtat caagcgtcac ttctacttcc agtggtttgc cgttacgcag caggccaagc
    30781 ttcactttcg tgcccggctc tgtagtcgcg atacgagagc gcaactcagc aaagctattc
    30841 agcggtttgc cgttgaggct ggtaataata tcgcccgctt tgacgcccgc tttcgctgag
    30901 ccagaacttg gcaacacttc gctgacaaac gcgccacgct gcacgtcaag gttgaaggct
    30961 ttggcgatat cggcactcat ctcggtgcct ttgatgccta acaaaccgcg tttgatttca
    31021 ccaaagtcga taagctgctg cgccagtgtt cgcgccatat tactggggat ggcaaatcca
    31081 atcccgacgc tcccgccgcc aggcgcaagg attgcagtgt tgatgccaat taactcaccg
    31141 ttaaggttta atagtgcacc gccggagtta ccgcggttaa tggaagcatc tgtctggata
    31201 aagttttcca gaccttcaag attcaacccg ctgcggccta atgcggaaac aatgccagag
    31261 gtggcggttt gcccaaggcc aaatgggtta ccgaccgcta cggcaaaatc accgacgcgc
    31321 aatttatcgg agtcggcaat agcgatttgc gttaatttgc tcgggttttg aatttgtaac
    31381 agggcgatat cgctctggtc atcgctacca atcagttttg catcaaactc gcgcccatca
    31441 ttgagctgaa tactgatttt ctgtgcctga ttaatcacat ggttgttggt cagcacatag
    31501 cctttactgg cgttgatgat gacaccggaa cctaaacctt cgaagggttg tgcaggttga
    31561 tccggtaaat catcaccaaa aaactttttg aattcttccg ggattttctg tccctgactg
    31621 gccgttcctt ccacccgtac gctcaccact gccggaagca ctttttccag cattggagcc
    31681 agactgggga gaggggcctg atcggcaacc tggcctggaa tcgacgcgac ggcctgaaat
    31741 gacgccgaga gagttaaccc ggcacttaac gctaatgcac tcaacagctg ggtttgtttt
    31801 ttcattattc ctgctctcgt acctgaatga taagaaaaga gattcaaaac agtttgatgt
    31861 tattgaattt tcagacggct aacaatgaga caccagatta aatgatagtc ggggagggag
    31921 agaagaggag ggcgcaatgg ctgcgcccga aaaataaatt agtcgcgctt cgcgccagta
    31981 cgcagcaggc cggatgcgcc ttcagaatag tcgcgaggca tctgcaccgg tgcctgatcg
    32041 ttgctggctt cagactctgc cagacgatta cggaacgggt ttgcttcagc agacagttcc
    32101 ggcagcaggc tgctggagct ttttgccatg tgctgataca gctggcgata gtcgtgcgcc
    32161 atggtatcca gtaattccgc gctgcgggca aagtggctaa ccagctcttc gcgatactcg
    32221 tccagttcag ctttattctt ttccagttcg tactgcaacg cctgttgctg gcgtagttta
    32281 cgattaccaa aacgcatggc cacagcacca ataatgatgc cgacgactaa cccaattagc
    32341 gcatattccc aggtcatgaa catctcccgt tgtcttttgt ttccgtaggg tgttggcttc
    32401 aggctccatg cctgcggctg attatgccac tataaccgtt aattccacag aagtggaatc
    32461 ccgactgcat atcgcgtagt gtagaacggc ctttttttcg tcaaccgtga acaacggcgc
    32521 accgattatt caaggaataa caataagatc atgcaaagcg ttaccccaac atcgcaatac
    32581 ctgaaggcgc tcaatgaagg cagccatcaa cccgacgacg ttcaaaaaga ggccgtcagc
    32641 cgcctggaaa ttatttatca agaactcatc aatagcaggc caccagcccc caggacgagt
    32701 gggctaatgg cgcgggtcgg taagctgtgg ggtaaacgcg aagacacaaa gcatacgcca
    32761 gtgcgtggct tatatatgtg gggcggtgta ggacgcggga aaacctggct gatggacctt
    32821 ttctatcaaa gcctgccggg agagcggaaa cagcgcctgc actttcaccg ttttatgctg
    32881 cgggtgcacg aagagctaac tgccttacag gggcagaccg atccgctgga aattattgcc
    32941 gatcgcttta aagccgaaac tgacgtgctc tgttttgacg aattttttgt ttctgatatt
    33001 accgacgcca tgctacttgg cggtctgatg aaagccctgt tcacccgcgg tattaccctg
    33061 gtagcgacgt caaatattcc gccggacgaa ctttatcgaa atggcctgca acgtgcgcgt
    33121 tttctgcctg caatcgatgc cattaaacag cattgtgatg taatgaacgt ggacgctggt
    33181 gttgattatc gactgcgtac actcactcag gcgcatctgt ggctttcgcc actcaacgat
    33241 gaaacccggg cgcagatgga taaactatgg ttggcgctgg cgggggtgaa acgagaaaat
    33301 tcaccgacgt tagaaatcaa ccatcggcca ttggcgacaa tgggcgtcga gaaccagacg
    33361 ctggcggttt cttttactac gctgtgcgtc gacgcccgca gtcagcatga ctatattgcg
    33421 ctctcacgcc tgtttcacac agtaatgttg tttgatgtgc cagtcatgac gcggttgatg
    33481 gagagcgaag cgcggcgctt tattgcgctg gtggatgagt tttacgagcg ccatgtcaaa
    33541 ttagtggtga gtgcagaagt gccgctgtat gaaatttatc agggtgagcg gctgaagttt
    33601 gagttccagc gttgcctgtc acgtctgcaa gagatgcaaa gcgaagagta tctgaagcgc
    33661 gagcatttgg cgggttaaaa cctgccacaa atcacaaaaa ggggtcgatc tttgaccccg
    33721 acttctctat aatcctgcga ccccacgtta caagaaagtt tttttcccaa aactttttgt
    33781 gtgctggcat aggctattcg aaggggtagg tttgccggac tttgtcgtgt gaacctcaac
    33841 aattgaagac gtttgggtgt tcaccaacgt gtaactattt attgggtaag cttttaatga
    33901 aaacttttac agctaaacca gaaaccgtaa aacgcgactg gtatgttgtt gacgcgaccg
    33961 gtaaaactct gggccgtctg gctactgaac tggctcgtcg cctgcgcggt aagcacaaag
    34021 cggaatatac tccgcacgta gataccggtg attacatcat cgttctgaac gctgacaaag
    34081 ttgctgtaac cggcaacaag cgtactgaca aagtgtacta tcaccacacc ggccacatcg
    34141 gtggtatcaa acaagcgacc tttgaagaga tgattgctcg ccgtcctgag cgtgtgattg
    34201 aaatcgcggt taaaggcatg ttgccaaaag gcccgctggg tcgtgctatg ttccgtaaac
    34261 tgaaagttta cgcgggtaac gagcacaacc acgcggcaca gcaaccgcaa gttcttgaca
    34321 tctaatcggg attataggca atggctgaaa atcaatacta cggcactggt cgccgcaaaa
    34381 gttccgcagc tcgcgttttc atcaaaccgg gcaacggtaa aatcgtaatc aaccaacgtt
    34441 ctctggaaca gtacttcggt cgtgaaactg cccgcatggt agttcgtcag ccgctggaac
    34501 tggtcgacat ggttgagaaa ctggacctgt acatcaccgt taaaggtggt ggtatctctg
    34561 gtcaggctgg tgcgatccgt cacggtatca cccgcgctct gatggaatac gacgagtccc
    34621 tgcgttctga actgcgtaaa gctggcttcg ttactcgtga cgctcgtcag gttgaacgta
    34681 agaaagtcgg tctgcgtaaa gcacgtcgtc gtccgcagtt ctccaaacgt taattggctt
    34741 ctgctccggc agaaaacaat ttgcgaaaaa acccgcttcg gcgggttttt ttatggctaa
    34801 attctgaatc agcgtaaaaa atgggaagtt gctttttgct gccgtctgaa agacaggtaa
    34861 aacaaaaacc acatcgccaa taagggtcta agtcaactat ttcagactaa agcgcatctc
    34921 tttttcccca tttccggcat ccactcacca caaaggtcgc aaaatctggt aaactatcat
    34981 ccaattttct gcccaaatgt cgggtattgc tcattttttg tttgattttc gaacaaagag
    35041 agtcgttcct tattgggtaa cacaacttct gactggccac ctggccacct ggtggctggt
    35101 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35161 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35221 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35281 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35341 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35401 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35461 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35521 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35581 tcctgagcga tgagttcagt ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35641 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35701 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35761 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35821 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35881 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35941 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    36001 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36061 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36121 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36181 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36241 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36301 gttttgtctt tttaatgact taa
//
LOCUS       DADLSE010000025        35876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLSE010000025 DADLSE010000000
VERSION     DADLSE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.025"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN692_001251"
     CDS             <1..246
                     /locus_tag="JN692_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263610.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN692_001252"
     CDS             complement(293..1051)
                     /gene="deoR"
                     /locus_tag="JN692_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7263611.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN692_001253"
     CDS             complement(1109..1705)
                     /gene="ybjG"
                     /locus_tag="JN692_001253"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7263612.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1990..3221
                     /gene="mdfA"
                     /locus_tag="JN692_001254"
                     /pseudo
     CDS             1990..3221
                     /gene="mdfA"
                     /locus_tag="JN692_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(3262..3546)
                     /locus_tag="JN692_001255"
     CDS             complement(3262..3546)
                     /locus_tag="JN692_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263613.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3632..4447)
                     /gene="ybjI"
                     /locus_tag="JN692_001256"
     CDS             complement(3632..4447)
                     /gene="ybjI"
                     /locus_tag="JN692_001256"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7263614.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4447..5655)
                     /locus_tag="JN692_001257"
                     /pseudo
     CDS             complement(4447..5655)
                     /locus_tag="JN692_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5739..6275
                     /locus_tag="JN692_001258"
     CDS             5739..6275
                     /locus_tag="JN692_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7263615.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6450..8135)
                     /locus_tag="JN692_001259"
     CDS             complement(6450..8135)
                     /locus_tag="JN692_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7263616.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8405..8782
                     /locus_tag="JN692_001260"
     CDS             8405..8782
                     /locus_tag="JN692_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263617.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8812..9069)
                     /gene="grxA"
                     /locus_tag="JN692_001261"
     CDS             complement(8812..9069)
                     /gene="grxA"
                     /locus_tag="JN692_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7263618.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9229..9516
                     /locus_tag="JN692_001262"
     CDS             9229..9516
                     /locus_tag="JN692_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7263619.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9500..10222
                     /gene="nfsA"
                     /locus_tag="JN692_001263"
     CDS             9500..10222
                     /gene="nfsA"
                     /locus_tag="JN692_001263"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HAY7263620.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10283..11185
                     /gene="rimK"
                     /locus_tag="JN692_001264"
     CDS             10283..11185
                     /gene="rimK"
                     /locus_tag="JN692_001264"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7263621.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERWAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11273..11749
                     /locus_tag="JN692_001265"
     CDS             11273..11749
                     /locus_tag="JN692_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7263622.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12101..13213
                     /gene="potF"
                     /locus_tag="JN692_001266"
     CDS             12101..13213
                     /gene="potF"
                     /locus_tag="JN692_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7263623.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13308..14441
                     /gene="potG"
                     /locus_tag="JN692_001267"
     CDS             13308..14441
                     /gene="potG"
                     /locus_tag="JN692_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7263624.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14451..15404
                     /gene="potH"
                     /locus_tag="JN692_001268"
     CDS             14451..15404
                     /gene="potH"
                     /locus_tag="JN692_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7263625.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15401..16246
                     /gene="potI"
                     /locus_tag="JN692_001269"
     CDS             15401..16246
                     /gene="potI"
                     /locus_tag="JN692_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7263626.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16306..16794
                     /locus_tag="JN692_001270"
     CDS             16306..16794
                     /locus_tag="JN692_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7263627.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16835..17962
                     /gene="rlmC"
                     /locus_tag="JN692_001271"
     CDS             16835..17962
                     /gene="rlmC"
                     /locus_tag="JN692_001271"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7263628.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18137..18868)
                     /gene="artJ"
                     /locus_tag="JN692_001272"
     CDS             complement(18137..18868)
                     /gene="artJ"
                     /locus_tag="JN692_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7263629.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19160..19828)
                     /gene="artM"
                     /locus_tag="JN692_001273"
     CDS             complement(19160..19828)
                     /gene="artM"
                     /locus_tag="JN692_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7263630.1"
                     /translation="MFEYLPELMKGLHTSLTLTFASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19828..20544)
                     /gene="artQ"
                     /locus_tag="JN692_001274"
     CDS             complement(19828..20544)
                     /gene="artQ"
                     /locus_tag="JN692_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7263631.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSYGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20551..21282)
                     /gene="artJ"
                     /locus_tag="JN692_001275"
     CDS             complement(20551..21282)
                     /gene="artJ"
                     /locus_tag="JN692_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7263632.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21300..22028)
                     /gene="artP"
                     /locus_tag="JN692_001276"
     CDS             complement(21300..22028)
                     /gene="artP"
                     /locus_tag="JN692_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7263633.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCSVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22246..22761)
                     /locus_tag="JN692_001277"
     CDS             complement(22246..22761)
                     /locus_tag="JN692_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7263634.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23410..25179
                     /locus_tag="JN692_001278"
     CDS             23410..25179
                     /locus_tag="JN692_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7263635.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25390..25713
                     /locus_tag="JN692_001279"
     CDS             25390..25713
                     /locus_tag="JN692_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7263636.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25710..26540
                     /locus_tag="JN692_001280"
                     /pseudo
     CDS             25710..26540
                     /locus_tag="JN692_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(26537..27550)
                     /locus_tag="JN692_001281"
     CDS             complement(26537..27550)
                     /locus_tag="JN692_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7263637.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27649..29079)
                     /locus_tag="JN692_001282"
     CDS             complement(27649..29079)
                     /locus_tag="JN692_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7263638.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29090..30091)
                     /gene="ltaE"
                     /locus_tag="JN692_001283"
     CDS             complement(29090..30091)
                     /gene="ltaE"
                     /locus_tag="JN692_001283"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7263639.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSRILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30128..31846)
                     /gene="poxB"
                     /locus_tag="JN692_001284"
     CDS             complement(30128..31846)
                     /gene="poxB"
                     /locus_tag="JN692_001284"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7263640.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTKFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31979..32947)
                     /gene="hcr"
                     /locus_tag="JN692_001285"
     CDS             complement(31979..32947)
                     /gene="hcr"
                     /locus_tag="JN692_001285"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7263641.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32959..34611)
                     /gene="hcp"
                     /locus_tag="JN692_001286"
                     /gene_synonym="priS"
     CDS             complement(32959..34611)
                     /gene="hcp"
                     /locus_tag="JN692_001286"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7263642.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34755..35654)
                     /gene="lysO"
                     /locus_tag="JN692_001287"
     CDS             complement(34755..35654)
                     /gene="lysO"
                     /locus_tag="JN692_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7263643.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8931 a         8879 c         9221 g         8845 t
ORIGIN      
        1 gtgaccatac cgcgtgggca gctgaaaaac ctgaaggcga gctacacgtt aacggaaccg
       61 cagcttaccg caccgctgaa aaaaggtcag gttgtcggga ccattgattt ccagcttaac
      121 ggtaaatcca ttgagcagcg tccgctgatc gtgatggaaa atgtggaaga gggcggattc
      181 tttggtcgga tgtgggattt cgtgatgatg aaattccatc agtggttcgg cagctggttc
      241 tcttaatctt ctgataaccg gatggcgcga aacgtcatcc ggttatacgt cattaataca
      301 tcaacttaat acggtgcgtc tgcgcgtact tcacatactc atcttccggg caacaatcac
      361 tcaccacaat atcaaagcgt ttcaggtcac ccatgcgcgc cggacgcacc ttgccaaatt
      421 tactgtggtc gacaaccagc acatgctttt gcgccatcga catggcccag tgttttaccg
      481 gcaactcttc aagattaaaa caggtagcgc ctttactgac atgcacgccc gccgcggaat
      541 aaaaagcgat atccgggcaa aaattattca acgtttgctg aaaatcgatg ggtttgaaaa
      601 tggcgttgct ggcgtgaaat tcaccaccgc acagaaatgc gcggcaatgg ggtttctctt
      661 tcagcgccag aaaggtattt agcgaataac aaacggcggt aaaagggatt tcattatcaa
      721 tcgcttcaat aatccacggc gtggtggtgc cacagtcaaa aaagagggtc tgatcgggtt
      781 ctaccagtgt cgcagccagt tttgccgccc ggcgtttttc ttccaccagg cgggattttt
      841 gatcgcttaa caggtaatgg ctggcactgc gcggttccag aacaatatag ccgccgagca
      901 aaacgacggg cgcactgtgg ttgttcagat cgcgacgaat cgtcatctcc gaaaccccaa
      961 gcagggcggc ggcgtcttta agatgtaact tatcgctgcg ttttaattct tgcagcagct
     1021 gcccgatacg ctcttcgcga cgtgtttcca taatccctct gaatagttat tgaagcgagc
     1081 cgctcaatac tacacttttt agcagagatc agtcacgcac ccagcctttg cggatcggta
     1141 atgcaaaaca gacgcgatac cacgattgca gacgttgata gagtttatgc cccatcgctt
     1201 gccagataat ctgggcacta aggcagccaa tcatacctgc cagcaatcca ccgagcatat
     1261 ccagcggcca gtggacgcca agataaacgc gcgaccaggc aatgacgacg gccagcacca
     1321 ttaaaagtga gccggaccac aggcgatgcc agcataaaaa tgccagtgca aaggtgaaaa
     1381 tcaccgtacc gtgatcgctc gggaatgagt catccgccgc atgatgcagg aagttatagc
     1441 cgatattttc gacaaagggt cggtcgtgcg gaaaaagatg tcccatcgtc caggacacaa
     1501 acaggctgac cgccagcgcg atagcgattt ttatcaccag ttgccgttgt gctgtaagcc
     1561 cccacaacca aagtaccgcg gccagcaacg gcaccacggt aatcaaatct ttagcaataa
     1621 aaatcgccaa cgagatcatc cacggagccg agtctggcgt cgcgttaata agagagaata
     1681 gagagagatt caaattttcc agcataactt cccgacgcaa agtgattaaa agggagccaa
     1741 tacaggcaag tcgttgagaa taaagtgcag gttaaactgg gtaaagcggc atcgtcttat
     1801 ttccctcaag cggcctgttt acggtgggtg attgtaacgg gcataggtta aataaaactt
     1861 aaagaaagcg tagctatact cgcaataatg taagaatgtg cttaaccgtg gtttcagcta
     1921 caaaattcgc tttctcgtta gctgcgcttt tattaaactc tgcgcgatta ttattggcga
     1981 agaaattgca tgcaaaataa attagcttcc ggtgccaggc ttggacgtca ggcgttactt
     2041 ttccctctct gtctggtgct ttacgaattt tcaacctata tcggcaacga tatgatccaa
     2101 cccggtatgt tggccgtggt ggaacaatat caggcgggca ttgattgggt tcctacttcg
     2161 atgaccgcgt atctggcggg cgggatgttt ttacaatggc tcctggggtc gctgtcggat
     2221 cgtattggtc gccgtccggt gatgctggcg ggtgtggtgt ggtttatcgt cacctgtctg
     2281 gcaatattac tggcgcaaaa cattgaacaa ttcaccctgt tgcgcttctt gcagggcata
     2341 atcctctgtt tcattggcgc tgtgggatac gccgcaattc aggaatcctt cgaagaggcg
     2401 gtttgtatca agatcaccgc gctgatggcg aacgtggcgc tgattgctcc gctacttggt
     2461 ccgctggtgg gcgcggcgtg gatccatgtg ctgccttggg aggggatgtt tgttttgttt
     2521 gccgcattgg cagcgatctc ctttttcggt ctgcaacgag ctatgcctga aaccgccacg
     2581 cgtataggcg agaaactgtc actgaaagaa ctcggtcgtg actataagct ggtgctgaag
     2641 aacggccgct ttgtggcggg gcgctggcgc tgggattcgt tagcctgcca ttgctggcgt
     2701 ggatcgccca gtcgccgatt atcatcatta ccggggagca gttgagcagc tatgaatatg
     2761 gcttgctgca agtgcctatt ttcggggcgt taattgcggg taacttgctg ttagcgcgtc
     2821 tgacctcgcg ccgcaccgta cgttcgctga ttattatggg cggctggccg attatgattg
     2881 gtctgttggt cgctgctgcg gcaacggtta tctcatcgca cgcgtattta tggatgaccg
     2941 ccgggttaag tatttatgct ttcggtattg gtctggcgaa tgcgggactg gtgcgattaa
     3001 ccctgtttgc cagcgatatg agtaaaggta cggtttctgc ggcgatggga atgctgcaaa
     3061 tgctgatctt taccgtcggt attgaaatca gcaaacatgc ctggctgaac gggggcaacg
     3121 gactgtttaa tctcttcaac cttgtcaacg gaattttgtg gctgtcgctg atggttatct
     3181 ttttaaaaga taaacagatg ggaaattctc acgaagggta aaaaaatgcc tgattgattt
     3241 gtgcgatcag gcattctcga attaatggtg atggtcgtca atctggtgtt cgataaccat
     3301 cccttcacct acgctggcaa gatggcgaac ataaggatgc gggcggtaag ccggagctgg
     3361 cgcaggagcc acatagacgg tttgcggcac agtcgcgacg ctgaccgctt gtggaacgct
     3421 gactgaatca ggaaccacaa ccactttgta tccactcggc acatcgacgg tgacactttg
     3481 cgccatcgcc acaccagtaa agcccattaa aagcgcgccc aacagtagac aatttttcat
     3541 aatcatttcg cctgaaataa tgcagcgggt taatttccgc gaattatgca gccttcattc
     3601 aggcattgtg ttgccgggcg tgctgccgtt tttattggtc aaatggcgct tcgtgtttaa
     3661 gaactttatc gatcacatcc agtacgcctt cacggttatt ggagcctgcc cggtattttg
     3721 ccgctgcgac gaccgcgctg ccggcatttt ccattgcaaa actaaagcct gcctgacgca
     3781 gcatctcaat atcgttaccg ccatcgccaa agaccaccac ttcgctgtcg tctattcccc
     3841 ataatttctg cagttggcga aggccattgg ctttatgtac gccggggata atcagatcga
     3901 tgctgccgtt gccggtgtgg actggcacca taatatcgcc gatggcctca tgtaatgctt
     3961 tttgtacttg tggaatcagt tcatcggaaa gattcaggcc aaacttaaag aaaatatctt
     4021 ctaagttgtc aaagttatcg acgtattcca gacggtgata atacatttcc gccaccgttt
     4081 tcatggcatc gtcatatttt ttgagtgtat aggcgctatt ttttccgcag gcaataattt
     4141 ccacttccgg gcgcgtcagc aaatgttcca cgacagtagc aaacgcatcc ttcgacagct
     4201 cgccattaaa aacatctttg ccttcactca ctacccagcc gccgttttca gccacaaagg
     4261 caatttcatt cgcaatttca gggaagaaag agatcaactg ataatattga ttcccgctgg
     4321 cgaccacaaa gcgaatgcct tgtgctttca tttgctgata ctgagccata aaccgctcac
     4381 ggttataggt tttttgatcg cttaagaaag taccatccat gtctaccgca attaatttaa
     4441 tgctcatcaa ctattctcca tcgccgtctg cgttttggta tcgggtttgg cgacggcttt
     4501 cgcgacaata gccgcgagaa taaccagcgc cagtacaacc agcattgcac tacgtaatcc
     4561 ataatgttcg ccgagatagc ccagcagcgg cggcccgacg aggaaagcca gataaccggt
     4621 cgttgctacc acactgacgc gggttggtgc atcggggccg gtatcgctgg cggcagaaat
     4681 ggtcagcggg aagcccagcg aggcacccag tccccaaagt acaacagaca ctcctgcgac
     4741 ccaggcgcta tcgacaaaaa taatcagccc aatacccaac gcccccatta gtgcactggc
     4801 ccgaaccacg gcaacgcgac tgtaacggtc gatgaaccaa ccgccggtga agcgtccaac
     4861 ggtcatccct agggtaaaac cggcataaat cagtgagccg gaagtggggc taaaaccgtg
     4921 accatcaacc attaataagg gtaactagtc gttggcagaa ccttcggcaa aggccatcgc
     4981 cagcactaca acaccaatca acagcaactg gatatcgcga taaaaaggaa tgcctttttc
     5041 gccatgctgg gtgccatcgg cagcattttt gcccgtaccg tcagggattg cctgaatggc
     5101 gatataaata ggcgcgatac ctaccagcgc cgccaataaa atgtgcaccg ttgccggaac
     5161 gccaaaggcc gtcagtgcca tcccgacacc agcgcctgcc agcgtgccca ggctataaaa
     5221 accgtgcatc atcggcaaaa ccgttttatt catttctcgc tcaacggcgg caccttcaac
     5281 gtttatcgcc acttccgcag aaccaaaact tgccccaaag acgccgagac caacggcaaa
     5341 gagcaggggc gatgtcagcc agagtgccag acttaatatc atcatcccga tcaatgcgca
     5401 ggacatcgtg accaggatga cattacgtgt cccaaagcgt ttcactaacc acgccgagca
     5461 gagaataccg ctcatcgaac cgatcgacag accaaagaga acaccgccca tttcagcgat
     5521 cgagacagag agaatatcgc ggatagcagg cgtacgggtt gcccaggacg ccattaacag
     5581 gcctggcaaa aagaagaaca taaacagcgc ccaggttcgg cgtttcaatg cattacgtga
     5641 agaatttacg gtcatagatc acgtcaaaat aagaagaggg aagacaacat tagcaaggtt
     5701 gtgtacattt gtacacaatt gcagacagag gaaatgacat gcgtcgcgct aacgatccgc
     5761 aacggcgaga aaaaattatc caggccacgc tggaggcggt gaaactttac ggaatacatg
     5821 ctgttacgca ccgcaaaatt gctacccttg ccggggtacc gttgggatcg atgacctact
     5881 atttttcagg aattgatgag ttgttgctgg aggcgttcag cagttttact gagattatgt
     5941 cccggcaata tcaggcattt tttagcgatg ttagtgatgc tcaaggcgca tgccaggcta
     6001 tcaccgatat gatctacagc tcacaggttg caacgccgga taatatggag ctgatgtacc
     6061 agctctacgc gctggctagc cgaaaaccgc tattaaaaac ggtaatgcaa aactggatgc
     6121 agcgcagtca gcaaacgctc gaacaatggt ttgaacccgg aaccgcccgc gcgcttgatg
     6181 cgtttattga ggggatgacg ctgcattttg tcaccgaccg taagccgcta tcgcgcgagg
     6241 agattttgag gatggttgag agggttgcag ggtagtagat aaattttaga taacaaaaaa
     6301 cccatcaacc ttgaaccgaa atggcggggt tgatgggctc cacaaaatgg ggacatcaaa
     6361 gaaaagcagt ggcactaatt aagactgatg ccctgcggaa aagttctgcg gttgtgcaaa
     6421 aaaatttcat tttcagggca acttcagttt tatcctaatc ctggccatac catgacgatg
     6481 attgtccctg ccagcgtcag caggacgttg gcgattgcgt aggtgcccgc atagcccagc
     6541 gcagggatgt tactgcgagc tgtatcactg atgatctcca ttgccggcgc gcaggtgcgt
     6601 gcgcccatca ttgcgccgaa caacagtgcg cggttcattc gcaatacata agcaccgaac
     6661 aagaaacaga taaccacggg cacaagactg acaattaatc cggcaatcaa catctgaccg
     6721 ccaatcgcgc ccaggccgtt attaataccg ctaccggcgc tcagaccaac gcctgccata
     6781 aacaccatca agccgaactc tttcaccatg cttaatgcac cttgcggaat gtaaccgaag
     6841 gtcgggtggt tagcacgcat aaagcccagc ataattccgg cgaataacaa cccggcagcg
     6901 ttccccatgc cgaaactgaa tgtgctgaac tggaaggtga tcatcccgat catcagccca
     6961 ataacaaaga aggcgcaaaa tgccagcagg tcagtgacct ggctgtgaat cgagataaag
     7021 ccgatgcgat cggcgatggt ttttacgcga cgggcatcac cgctgacttg taaaacgtca
     7081 cctttgttaa gtacgacgtt gtcatctatc ggcatctcaa tctggctacg aatgacgcgg
     7141 ttaaggaagc aaccgtgatc ggtcaacttc agttgtgcga gacgtttacc tacagcgtta
     7201 tggtttttaa cgaccacttc ttcagtgacg atacgcatgt cgagaaggtc acgatcgaaa
     7261 acttctttac cgttacggaa gctgggatcg agtcgggcat gggcgtcggg atagcctacc
     7321 aacgctattt catcgcccat ttgtagcacg gcatcaccgt ctggatttgc cagaatcccg
     7381 ttacgtcgaa tacgttcaat gtagcagccg gtttgtcgat aaatacccag ttcacgcaga
     7441 tttttgccgt cggtccaggc caccagctcc gggccgacgc gataggcgcg gatcaccggt
     7501 aaataaacct tacggttggc atcagtgtcc aggccacgtt cgcgggcgat ttgctgggcg
     7561 ctggtctgta agtcctgatg ctgcaatttc ggcaagtaac gcgcaccaac aatcaaactc
     7621 accagaccga ttaaataggt taaggcatac ccgaggctca gattatccag tgccagtgag
     7681 agctgcctgc tttccatgcc ggaatgacgc agtgtatcgc cagcaccgac cagaaccggt
     7741 gtcgacgtca tagagcctgc taacataccg gccgtcaggc caatatccca gccaaacagc
     7801 ttacctaacc ctaaggcgat caccagcgca ctgccaacca tcaccagtgc taacattagg
     7861 taatttttcc catcgcgaaa aaaaatggaa aaaaagttcg gtccggcttc gaccccgacg
     7921 cagaaaataa acagcataaa gccaagatta agcgcatcgg tgttaatgct gaaatgttgt
     7981 tggcctaata acagcgatac gactaaaacg ccaatggaat tacccagttg gatcgaacca
     8041 agtcgtaact ttccgagaca tagcccaagc gcgaggacca caaataataa cagaatgtaa
     8101 ttcccattta acaattcggc gacgtttata ttcacggagg ctaacttctt gtttactagt
     8161 aagctgttga aagaaatggt aatttacgat aatgtttttt accagaattc agggcgcaga
     8221 ttcattcagc gcacctaaac gatagtaaag taacaatata ttttactagt gtaatcacat
     8281 taggtatcaa cggctatatg aattgcgttg gcctatatta gcatggaatg cgaagcggct
     8341 ttatcttact gaacgccaca ctggcgaaaa atgtgttcga tagacgcagt gtcaggagga
     8401 acgagtgaaa cataaacaac gttgggcggg ggcaatctgc tgttttgtcc tcttcattgt
     8461 ggtgtgcctt tttctggcga cgcacatgaa aggcgctttt cgggctgccg ggcatcctga
     8521 aatcggcttg ctgtttttca ttcttcctgg agcagttgcc agtttctttt cacagcgtag
     8581 agaagtcctg aaacctctat ttggcgcaat gctggcggca ccctgttcaa tgctcattat
     8641 gcggctgttt ttttcaccga cgcgctcatt ctggcaagag ctggcatggt tactaagcgc
     8701 ggtgttctgg tgtgcgctgg gggcactgtg tttcttattt atcagtagtt tgtttaaacc
     8761 acagcacaga aaaaatcagt aaagccctca acgcgagggc ttgtcagacg atcaggcgtc
     8821 cagattttct ttcacccatg cagcaaaatc ggtatagccg ccgatatgtt gctgatcgac
     8881 aaaaatctgc ggcacggttt ctacgggttt acctgccttt tgttgtagat cttctttagt
     8941 gatcccttcc gcacgaatat ctacatactg atactgaaaa tcatcgcgtt cattgctcaa
     9001 tttctcagcc agatcttttg cacgcacaca gtaagggcaa cccgaacgac caaaaataac
     9061 ggtttgcatt atttctctcc tcatagattt atgcctgtaa tgatcacgct aaaatgtatt
     9121 cgctgaaagt aggtttaacc tgttgcatta attgctaaaa gctataactg ttaaacacaa
     9181 tacagtgaaa agttttagac tgaaggctca ctttgcagag ggaagcgtat gcgcgcgatc
     9241 ggtaaattgc ctaaaggcgt gttgatactg gaatttatcg gaatgatgct actggcggtg
     9301 gcgctgctgt cggtaagcga ctccctgtcg ctgcctgagc cattttctcg gccagaagtg
     9361 cagattctga tgatttttct cggtgttttg ctcatgcttc ccgctgcggt ggtggttatt
     9421 cttcaggtgg caaaacgtct tgccccacag ctgatgaacc gtccaccgca atattcacgt
     9481 tcagaaagag aaaaagataa tgacgccaac cattgaactt atttgtggcc atcgctccat
     9541 tcgccatttc actgatgaac ccatttccga agcgcagtgt gaggcgatta ttaacagcgc
     9601 ccgtgcgacg tccagttcca gttttttgca gtgtagtagc attattcgca ttaccgacaa
     9661 agcattacgt gaagaactgg tgacgctgac cggcgggcaa aaacacgtag cgcaagcggc
     9721 ggagttctgg gtgttctgtg ccgactttaa ccgccattta cagatctgtc cggatgctca
     9781 gctcggcctg gcggaacaac tgttgctcgg tgtcgttgat acggcaatga tggcgcagaa
     9841 tgcattaacc gcagcggaat cgctgggatt gggcggggta tatatcggcg gcctgcgcaa
     9901 taatattgaa gcggtgacga aactgctgaa attaccgcag catgttctgc cgctgtttgg
     9961 gctgtgcctt ggctggcctg cggataatcc ggatctcaag ccgcgtttac cggcctccat
    10021 tttggtgcat gaaaacagct atcaaccgct ggataaagac gcactggcgc agtatgacga
    10081 gcaactggcg gaatattacc tcacccgtgg cagcaataat cgccgggata cctggagcga
    10141 tcatatccgc cgaacaatca ttaaagaaag ccgcccattt attctggatt atttgcacaa
    10201 acagggttgg gcgacgcgct aaaaccgcca cgtcgatgta tgatacgcgg gcttttgacc
    10261 aggtctgaca gagaggtgca gggtgaaaat tgccatattg tcccgggatg gaacgctcta
    10321 ttcgtgtaag cggctgcgtg aagccgctat acagcgcggt cacctggttg aaattcttga
    10381 tccgctttct tgctacatga acataaatcc tgcggcgtct tctattcact acaaaggccg
    10441 caagttaccc cattttgacg cagtgatccc gcgtattggc accgccatta ccttttatgg
    10501 gacggcggca ctgcgccagt tcgagatgct ggggagctat ccgctcaatg agtcggtcgc
    10561 cattgcccgg gcgcgtgaca aattgcgttc catgcaactg ctggcgcgtc agggcatcga
    10621 cctgcctgtc acgggcattg cgcattcgcc ggatgatacc agcgatttaa tcgacatggt
    10681 cggtggtgcg ccgctggtgg tcaagttggt tgaaggcacg cagggaattg gcgtcgtgct
    10741 ggcggagact cgtcaggcgg cggaaagcgt gattgacgct ttccgcggtc tgaacgcgca
    10801 tattctggtg caggaatata tcaaagaggc gcaagggtgc gatatccgct gtctggttgt
    10861 tggtgatgaa gtggtcgctg cgattgaacg gtgggcgaaa gagggctatt ttcgttccaa
    10921 tttgcatcgt ggcggcgcgg caagcgtcgc cagtatcaca ccacaggagc gtgaaatcgc
    10981 gataaaagcc gcgcgaacga tggcgctgga cgttgctggt gtggatattc tgcgtgctaa
    11041 tcgcgggccg ttggtgatgg aagtgaatgc gtcgccgggg ctggaaggaa tagaaaaaac
    11101 caccggtatc gatatcgcgg gtaaaatgat ccgctggatc gaacgccacg ctacgacaga
    11161 atattgcctg aaaacgggtg gttagtcgca atcacattac tgatcatggt tttgcctgcg
    11221 ctttttgcgt aagctgtgcc ggtcttttta tcgaaagagg ttgtacaaaa ttatgacatc
    11281 gctggtcgtt cctggtctgg atacgctgcg tcaatggctc gatgacctgg ggatgagttt
    11341 ttttgaatgt gataactgtc aggctctgca tctgccccat atgcagaatt tcgacggtgt
    11401 ctttgatgcc aaaatcgatc tgatcgataa cacgatcctg ttttctgcca tggcggaagt
    11461 ccgaccttca gccgtattgc cgctggcggc ggatttatct gccatcaatg ccagttcgct
    11521 gaccgtgaaa gcatttcttg atatgcagga tgataatctg ccaaagctgg tggtttgcca
    11581 gtctttatcc gttatgcagg gcgtaaccta tgagcagttt gcatggttcg tgcgtcagag
    11641 cgaagagcag atttcgatgg tcattcttga agctaatgcc catcaactgc tgttaccgac
    11701 tgacgatgaa gggcaaaaca acgttaccga aaactatttt ctccactgat aattcctttc
    11761 gagcacgcag tcgctgccgc agtgactgcg tgctgcaaaa ttatctgctg tttttaacct
    11821 tttcttaaag attatttcac ttctcttgtg tcgatttggc tttatcacat agagcaaata
    11881 cgcataaaaa tttgttaaat accgtttttt aatccgagct atagtctcaa accctggcta
    11941 aagttattct tgcgatactt ttatatagcg agcagtgctg gccgggagag agttctcttt
    12001 tcttacaccg cgccgataaa aaaatatgca cgtttattgc atatctttca gtgtgacaac
    12061 ttttgttcgt ttgttaacga actttcagaa ggaaagagat gtgaccgcct taaataaaaa
    12121 atggctatcg ggtctggttg cgggtgctct gatggccgtc tctgtcggca cgctcgcggc
    12181 tgaacaaaaa acactccaca tttataactg gtctgattat atcgcccctg acacggtggc
    12241 caattttgaa aaagaaaccg gtattaaagt cgtctacgat gttttcgact ctaacgaagt
    12301 actggaaggc aaattaatgg cgggtagcac cggctttgac ttggtggttc catctgccag
    12361 ctttcttgaa cgccagttga ctgcgggagt tttccagccg ctggacaaaa gcaaattgcc
    12421 ggagtggaag aatctcgacc cggagctgct gaagctggtc gccaaacacg atcccgacaa
    12481 taaatttgct atgccctata tgtgggcgac gaccgggatt ggctataacg ttgataaagt
    12541 taaagctgtg ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc tcaaacctga
    12601 aaatctggaa aaactgaaaa gctgcggtgt ctctttcctg gatgcgccag aagaagtttt
    12661 tgctaccgtg ttgaattatc tcggcaaaga tcccaacagc actaaagcgg atgattacac
    12721 cggaccggca accgatctgc tgttaaagct gcgcccgaac attcgttatt tccattcatc
    12781 tcaatacatt aacgacctgg caaacggcga tatttgcgtc gctatcggct gggcaggtga
    12841 tgtctggcag gcgtcaaacc gcgcgaagga agcgaagaat ggagtgaatg tctcgttctc
    12901 gattccaaaa gaaggggcga tggcgttctt tgatgtattc gccatgcctg cggatgccaa
    12961 aaacaaagac gaagcctatc agttcctgaa ttacctgctg cgcccggatg tagtagcgca
    13021 tatttccgac catgtgttct atgccaacgc caataaagca gccacgccgc tggtgagtgc
    13081 ggaagtccgt gagaacccgg gtatttatcc gcctgcggat gttcgtgcga agctgttcac
    13141 tctgaaagtg caggacccga aaatcgaccg tgtgcgcacc cgcgcgtgga ccaaagtgaa
    13201 gagcggaaaa taatccgcag ccttagatgc cgggcgggcg caccacaccc gccggcaatt
    13261 cgcaccatca tggtgcgctt gcacacattc aatgccggag agcagccgtg aatgacgcta
    13321 tccctcgccc gcaggcgaaa acccgtaagg cgctgacgcc gctattagaa atccgcaacc
    13381 tcaccaaatc ctacgatggt caacatgcgg tggatgatgt cagcctgacc atctacaaag
    13441 gtgaaatctt cgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg ctgcgtatgc
    13501 tggcaggttt cgaacaacct tctgccggac agataatgct tgatggcgtc gatttgtcac
    13561 aggttccgcc ttacctgcgc cccatcaata tgatgtttca gtcttacgcg ctgtttccgc
    13621 atatgaccgt ggaacagaac atcgcttttg gcctgaaaca ggacaaacta ccgaaagcgg
    13681 aaatttccag tcgggtcaat gagatgctcg gcctggtgca catgcaggag ttcgccaaac
    13741 gtaaaccgca tcagctttct ggtggtcagc gacagcgtgt ggccctggct cgaagccttg
    13801 ccaagcgccc gaaactatta ctgctcgacg agccgatggg cgcgctggat aaaaagctgc
    13861 gtgaccggat gcagcttgaa gtggtggata ttctggagcg cgtcggtgtg acttgtgtga
    13921 tggtcaccca cgatcaggaa gaggcgatga ccatggcggg gcgcatcgcc attatgaatc
    13981 gtgggaaatt tgtccagatt ggcgaaccgg aagagatcta cgagcatccg actacccgct
    14041 atagcgccga atttattggc tcggtaaacg tctttgaagg cgtactcaaa gagcgtcagg
    14101 aagatggcct ggtgcttgat tcgccggggc tggtgcatcc actgaaagtc gacgcggatg
    14161 cctcggtggt cgataacgtg ccggtacatg tggcgctgcg cccggaaaaa atcatgcttt
    14221 gcgaagagcc gcccgccaat ggttgtaact tcgcggtggg ggaggtgata cacattgcct
    14281 atctcggcga tctttcggtg tatcacgttc gtctgaaaag tgggcagatg attagcgccc
    14341 agctacaaaa cgcccatcgt catcgtaaag ggttaccgac ctggggcgac gaagtgcgtt
    14401 tgtgctggga agtggacagc tgtgtggtgc tgacggttta aggagcaaag atgaatacac
    14461 ttgaacctgc tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg tcgcagctgc
    14521 aaatgaagca tgggcgcaaa ctggtcattg cgttgccata tatctggttg atcttgctgt
    14581 ttctgctgcc atttctgatt gtctttaaaa taagcctggc ggagatggcg cgcgctattc
    14641 caccttatac cgaactgatg gagtgggctg acgggcaact gtccatcact cttaatctcg
    14701 gtaattttct gcaactgacc gacgatccgc tctatttcga tgcttatctc cagtcgctac
    14761 aggtggctgc gatttcgaca ttttgctgtt tactgatcgg ctatccgctg gcgtgggcgg
    14821 tggcgcacag taagccttcg acccgtaata ttttattact actggtgatc ctgccgtcgt
    14881 ggacctcgtt tctgatccgc gtttatgcct ggatgggaat attaaaaaac aacggtgtgc
    14941 tgaataattt tctgctgtgg ctgggggtta tcgatcaacc gctgaccatt cttcatacca
    15001 atctggcagt ttatattggc attgtttacg cctacgtgcc gtttatggtg ctgccgattt
    15061 ataccgcgtt gattcgtatt gattattcgc tggtggaagc agcgctggat ctcggtgcac
    15121 gaccgctgaa aacgttcttt actgtgatcg tgccgctgac taaaggtggg attattgccg
    15181 gatcgatgct ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg
    15241 gcccggacag catcatgatc gggcgcgtgc tatggcaaga gtttttcaat aaccgcgact
    15301 ggccggtggc ctcggcggta gcgatcatca tgttgctgct gctaattgtg ccgataatgt
    15361 ggttccacaa acaccagcaa aaaagcgtgg gagaacacgg atgaataatt taccggtagt
    15421 tcgttcgccc tggcggattg tgattttgct gctgggcttt acctttctct acgcgccaat
    15481 gctgatgctg gttatctatt cgtttaacag ctcgaagctg gtgacggtgt gggccggctg
    15541 gtcaacgcgc tggtatggtg agttattgcg cgatgacgcg atgatgagtg cggttggttt
    15601 aagcctgaca attgctgcct gtgcggcaac ggcggcggcg atcctcggga ctattgcggc
    15661 ggtggtgctg gtgcgctttg gcaggtttcg cggatcaaat ggctttgcct ttatgatcac
    15721 cgcgccgctg gtaatgccag atgtcatcac gggcttgtcg ctgttgttat tattcgtcgc
    15781 gcttgctcat gccattggct ggcctgcgga ccgcggtatg cttaccatct ggctggcgca
    15841 tgtcactttc tgtacggctt atgtggcggt cgttatttcg tcgcgtctgc gggaactgga
    15901 tcgctcgata gaagaagccg cgatggatct cggtgcgacg ccgctgaaag tgtttttcgt
    15961 cattacgcta ccgatgatca tgcccgcgat catttctggc tggttactgg cttttacttt
    16021 gtcgcttgat gatctggtga tcgccagctt tgtttccggg ccgggagcca ccacgttacc
    16081 gatgctggtc ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg ccttggcaac
    16141 attgatcctc ggcgcggtcg gaattgtcgg atttatcgcc tggtatctga tggcgcgcgc
    16201 agaaaaacag cggatacgcg acatccaacg tgcaagacgt ggctgaagac actaaaattt
    16261 gccaacctgg ctacataatg ccgcgcatgt cgcggcattg ttttcatgga agacgaaacg
    16321 ttgggatttt ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg
    16381 caggtggcgg cgctcgccat tattatgatc cgtggcctcg acgtgctgat gattttcaat
    16441 acgctgggcg tgcgcggtat tggcgagttc attcatcgca gcgtacaaac ctggagttta
    16501 acgctggtct ttttaagcag tctggtgctg gttttcattg agatctggtg tgcgttttca
    16561 ctggtgaaag ggcgtcgctg ggcgcgctgg ctatatctgc tgacacaaat caccgccgca
    16621 agttacttgt gggcggcttc gctggggtac ggttatccgg agctgttcag cattcccggt
    16681 gaatcaaaac gtgaaatctt ccatagcctg atgctgcaga agctgccgga tatgctcatc
    16741 ctgatgctgc tgttcgttcc ctcgaccagt cggcggttct tccagttgca ataatgtgta
    16801 taatcgtcgc ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg
    16861 tcgctgtcgt tcctgtcagt ggataacgca gccgattcca gagcaactct ccgctaaaac
    16921 cgccgatctt aaaaatctgc tcgccgattt tccggttgag gaatggtgcg cgccggtgtc
    16981 aggcccggaa caagggtttc gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa
    17041 accactgctc ggtatgctgc atcgcgatgg cacgccggaa gacctttgtg actgcccgct
    17101 ttatccagcc tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg cccgagcggg
    17161 gttaacgccc tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc tgctgactga
    17221 aagccagagt gatggcggca tgatgctgcg ctttgtactg cgttctgata ccaagctggc
    17281 gcaactgcgt aaggcgctgc cgtggttaca cgaacaacta ccgcagctga aagttattac
    17341 cgtcaatatt cagccggtac atatggcgat tatggaaggg gagacggaga tctacctgac
    17401 cgaacaacag gcactggcgg agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag
    17461 tttcttccag actaatccgg cggtcgccag ccagttgtac gccaccgcgc gcgactgggt
    17521 acgacagctg ccggttaaac atatgtggga tctgttctgc ggtgtggggg gctttggttt
    17581 acactgcgcg acgcctgaca tgcagttaac cgggatcgaa attgcgccag aggccattgc
    17641 ctgtgcaaag cagtcagccg ctgaactggg cttaactcgt ttacaatttc aggcgctgga
    17701 ctccactcag tttgccactg ctcaggggga ggtgccggag ctggtgctgg ttaacccgcc
    17761 gcgccgcggc attggtaaac cgctgtgtga ttatctttca acgatggcac cgcgttttat
    17821 tatctactcc agctgtaacg cccaaaccat ggcgaaagat atccgcgaac tgccaggtta
    17881 ccgcatcgaa cgggtacagc tttttgatat gttcccgcac accgcgcact atgaagtgct
    17941 gacgctgctg gtgaagcaat aaaaaagcca caggtgcggc ttcagattgc tgatcaggtg
    18001 cgctttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcatgctaaa
    18061 tcaatacatt gcagagttga tgtgggccgg ataagcgcag cgcatcaggc aatcttactt
    18121 ttgtcatcag tctcaattac tgcggaaacc actggtcatt gattttctga tatgtaccgt
    18181 cagctttaat tgctgccagc gcgttattca gtttttccag cagagctttg ttatccggac
    18241 gtacagcgat acccaggccg gtgccgaagt attgcggatc ggtcactttt tcagtcgcaa
    18301 cgcccagttg cggattggtt ttcagccatt cgtttaccac cgcggtgtca ccaaataccc
    18361 catcaatacg accatttttc agatcgataa aggcattctg ataactgtca taggagacag
    18421 ttttcacttc cgggtgctga tcctgaatat atttctggtg cgtcgtaccg ttttccatcc
    18481 caatgcgttt gcctttcagg tcggcaaacg ttttgtaggt atcttttttg gcaatcacga
    18541 cggctgagtt ttcatagtag ggtgtggtaa acgaaacctg cttgctacgc tccggggtga
    18601 tatccatacc ggagattacg gcgtcatatt ttctgaattt cagtgacggg atcaggctgt
    18661 cgaacgcgtg attagtaaaa gtacattctg cctgcatttg tttgcacaag gcttttgcca
    18721 gatcgatatc aaagccgaca atctcattat tagcacctat agattcaaag ggtggatagg
    18781 tggctgaaac gccaaaattg attttctctg cggcagaagc accgaaagta aaggaagcaa
    18841 gtaaagcggc aagaactaac tttttcatga tggaactccc gtctgtcaat cttatgattt
    18901 ttggccgtgt ctgcggcatg ggataacaat gccaccaagt gaatttatat gcaataaaca
    18961 tgattaaata atttaaatga aataaaaaaa gacggataac ttagtgagtt gtccgtcttc
    19021 attataagaa tttatgcact atgtaggccg gataaggcgt ccacgccgca tccggcacag
    19081 gcaccgtgct gatgtctgat gcgacgctgg cgcgtcttat cagacctaca aacccccccg
    19141 gcgaatgtac gcagctacat taattccgcc gttcgaatgc cagtgctttg cgctcgatca
    19201 gacgcatcat cagcgtcagc aggccgttga cgaccaggta aataatccct gccgcaccga
    19261 acaccattac atcgtaggtg cgtccgtaca acaactggct gtatcccatc acttccatca
    19321 gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg ttggaataag
    19381 aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct tttttgctca
    19441 ttcccagggc gctacaggac tgccactgac cttctgggat cgcacgaatt gcaccgtaaa
    19501 acagctgcgt ggtatacgcc gcactgttca gcgataacgc aatcagcgca cataaccacg
    19561 gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcggaaac tggcccggcc
    19621 cgtaataaat caggaagatc tgcaccagca gcggcgtacc ggtaaacagc gtgatataac
    19681 cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta aaaatcaatg
    19741 ccagaatcag tgccacaatc agcgaggcaa aggttagcgt caggctggtg tgcagccctt
    19801 tcatcagctc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac gtgtcgcgcg
    19861 caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt aaatcgccgc
    19921 cgccacaatg taccaggtaa atggttcctg ggtacgagta gcaatgcttt tggtttgtaa
    19981 cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca gcaccagcca
    20041 ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca gacggaaaaa
    20101 gatagccgat ttcgacagcc ccagcgcctg accagattcc cactggccca ccggtacagc
    20161 tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg acagagcgat
    20221 gacaccacaa aggaacggac tcacgtcgaa gttctcaatg tccatctgca ccgggatctg
    20281 cacgaaccca agattgatag tgaagccata cgaaagcgtc agcagtaact gcgaggagcc
    20341 aaaatagata aacagcacta ccagaatttc tggcaggcca cgcagaatgg ttaccagcgc
    20401 tgaacctgcc cacgcgacag gacgccattt cgctgactcc cataccgcaa agaacatcgc
    20461 cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc cggcggcgct
    20521 tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt tgtagatggt
    20581 ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct gctgcagttc
    20641 agtgttgccc tgacgtaccg cgatgccgag gccagtaccg aaataatctt tatcggtcac
    20701 tttatcgccc actgccgcca gttttgggtt atctttcagc cactcagtga ccactgcggt
    20761 gtcaccaaat acgctgtcga tacgcccgtt ttgcagatcc agttttgcgt tctggtagct
    20821 gtcatacgga acggtggtga tttccgggtg cttatccata atgaacttct ggtgtgtcgt
    20881 cccgttctga acgccgactt ttttgccttt cagctgatca acactggtgt atttgccttg
    20941 ctgacccaca aacagggcag agttgtcata gtacggggtg gtaaacagta cctgcttttc
    21001 acgctccgga gtgatatcca tgcccgccat cactgcttct acgcgacgga atttcaggct
    21061 tgggatcagg ctgtcaaacg cctggttaga gaaagtacag gttgcatcaa tctctttaca
    21121 cagtgcctgt gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat caatcgattc
    21181 aaacggagga taggaggctt cggtagcaaa acgaatggtt tcggcagctg tggcggaaag
    21241 actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta tcccgaatct
    21301 tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca gctcgcgtcg
    21361 ccttgttcta cgatatgacc attttccata tacaccactc ggctggcggt tttacgcgcc
    21421 acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag ctcacgaatg
    21481 atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc atcgaacagc
    21541 agtacctgcg gttccatcat caacgcacgg gcaatagcaa cacgctgctg ctgaccacca
    21601 gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc cagcagtttt
    21661 tctgcacggg ccagcgcctg atctttactc aaccccagta cactgcaggg cgcttcaatc
    21721 aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac catgccaacg
    21781 ttacgacgca aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc gaaatggttg
    21841 cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac acgcagcagc
    21901 gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg tgggcaatcc
    21961 agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc gtttaattga
    22021 atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa gatagtacct
    22081 ttgacagaat aattatgcaa tatttatgct ttaaaagtta aaagcaaagc gcattattca
    22141 ataaacatag cacaaaataa cgggggcggt ggtcggtgag cataaatgtc ggcattcctc
    22201 acgaaatgcc ggataattta cggggtttat tggttgatca aggcgttagc gattctcgat
    22261 ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc gcacatcgtc
    22321 gatgacccaa cactggcctt cctgaatcat taacacttca tcctgccagc cctggtcacc
    22381 ctgtttgaga tccacccgca atggaatgtt acgggcatca cgattaggga tagtcgatgc
    22441 actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat cgctgcttaa
    22501 caattcacga tgactattat cgcggctggc atcgctaagt tgggttgcca gtttgtcact
    22561 caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt agtcgtagaa
    22621 ttgttgagcc acactgtcag gccctccctg gacacaggaa ccggtgcggg ggccgttatc
    22681 tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg gaataagtag
    22741 agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc gtcacactac
    22801 gaccaaacgc tatcagatta tttaatacat tgtttttaat acaattctga aacaatctga
    22861 acacctgatt gggtttgagt ccattgctgg ctacccactc aaggttgctc gtagctgact
    22921 tacttaaatt acccaaacaa tagacagtgt aaaccatttg tgaggttgag cgtgttatat
    22981 ggctgaaatg atcatttaag gtaaatatga tagttctgaa atggtggtat aaatcctcct
    23041 gtagaggtta agagcatctg caagaagcca gtttcacatt tataacgcgt gataaatgaa
    23101 tgcggttatt gtctaatcat tacaaaattt ttcacatgcg ataagagatc ctgcgattaa
    23161 gatgattgcc aaaattacgt tgtatttttg aaaatgagcg aaatttgata gtcccagctt
    23221 tgactctcga taatattatt tatgctacaa ttgtgggtaa agaatttaag ctaatgaatt
    23281 tgagtgaaga ataaattaaa ttgcttcagt attttgttaa ttaatatgaa tacaaaaggc
    23341 gttgtgcttt tataattata aagcgcgagg agttcggtgg attatcgttt ttcttgggtg
    23401 atttttaata tgataaacaa tatatctgat caagcatcat catttccagg cacacaacta
    23461 aatcaaagtg ataatttcct ggattcactg agggaatttt tcgcgatatt aaattccagt
    23521 cgtaagggtg agttgtctac atgggatacc atctacttgc atctgatatt agcaataaat
    23581 gctgacagtg atctcataaa aaatgatgtg ttgctcgcgg aaaatattcc atcagcgaat
    23641 taccaattta atacattttt tagcaatgct ttcgagattg atgttaaaaa atatctgggt
    23701 aaatcagaag ataacgaagt tgaaataaaa gcgggtaatg aaagaatctc tattggaata
    23761 cgtaatgtat cgaatggaaa acttgaaaga caacagtttc ttttcccact ggactatgag
    23821 aataaattac aagaacaatt agataagtat tttacgattg aatcgcaccc attattatac
    23881 aggtatacca ttggtagtaa gattgcgaat gtgatttttg aaaagttata cagtcgtatt
    23941 gactttaaca aagaacaata tatatccttt ataaaggacg catttattca tttttatgat
    24001 tatagcaggc gatatgctat tagtgagaat atcgataaag acgctgtaac aaacaatatc
    24061 gcattgatga gcacttttta tgacagtgat aatacgtcag gtgaggtttt aaataatgat
    24121 tttacagaag aagagtcatt tgaaactgcg ttagatgtag agcatgcaat agtgcttgga
    24181 tttgctgatg ataattttga aacaaaacct gttcattatc aggatttatt aacacgattt
    24241 agtgcctttc aagataccgt gtttaatctg tttcctgaaa tgcactcttc gcattaccac
    24301 gatatttgta gcgtaagtgt ggatatgaca aagggaacgc aatgcatgat tcacttgatg
    24361 gtgaatgaag aagttttcat gtctctgcct gttccagttg ccacgatggt acgtgaagat
    24421 gcaagtaatt tagtcaatct taaaacacta ctcaatgatg gttgtttcat taaatatagt
    24481 cattttaatg acgtggcatt aataaagcaa aacataagta atctctattt atcacacact
    24541 gttgtgaatg aaagcatatt gaaaaaatgt tgttttgaaa acggcagttt aggtgatgtt
    24601 aaaataacaa atagcaatgt aataaacagt gcattcaaaa atatttcgtt tagatctgtt
    24661 aaaattaata acgtaaatac tcattcttta aaatttatta actgcaattt tttcaatgtt
    24721 gatatgataa gagttaatct cagcaaatgt ctttttcatg aatgtagtat gcatggtgtc
    24781 aagataaaac cctggttgcc tgtaaaatgg acaaaagaat taataagtga ttatttgtat
    24841 ggctgtttac tatctctgta ctctatctgt gcaagggata tatataatat gaacgccggg
    24901 aataacgtga aggttgctgc ggatgctttc cttgaaatta ttttctctct taaaaataaa
    24961 tattgtatta aactattaag cgctcaggat cgggcgttta tatacgagtt tgcaagaatg
    25021 atcttcgctt atattaatga caaaagcatt gagatcttat tactttcttg ttttgcggcg
    25081 gctgaccaaa aagcaatcca gcggtataga cctcagtcac aagatgggga agactttagg
    25141 agtcatttac aatataagtt gcctctttcc gctcattaat gttgttggtg caacataata
    25201 agacaggtaa gcggtaccct ataatgaaac caatatcggg ttatgcaacg gataatcgta
    25261 aagacttaag tgggtgtttt tgacacaatg agatgatttc ctaaactgat caaaaccttc
    25321 ttcccaacat ggcatatcag tccgataatg ctttagagat cgaaggctta acaaacaagg
    25381 agatcgatca tgcaattttc tacaacccca actctggaag gccagaccat cgttgaatat
    25441 tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga tttctttgcc
    25501 ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca
    25561 cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc cgatgccgta
    25621 gttggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct gatggttagc
    25681 gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg gtcgctgccg
    25741 ctctgttact ggcagggggt gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc
    25801 ttgatacccg acaccaggcg caggcggcgt atccgcgcat taaagtgctg gtgatccact
    25861 acaccgcaga tgattttgat agctcgctgg cgacactgac tgataagcag gtcagctcgc
    25921 attatctgat ccctgcggtg ccaccgcgat acaacggtaa accgcgcatc tggcaactgg
    25981 tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc
    26041 ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa tcatccggag
    26101 tgaaatattt tgccccgttt gaacaggcac agattcaggc gcttattccg ctggcgaaag
    26161 atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac
    26221 cgcagcgcaa agacgatccg gggccattat ttccctagca gcaactggcg cagcagggga
    26281 ttggtgcctg gccggatgcg cagcgggtga acttttacct tgccgggcgc gcgccgcaca
    26341 catcggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat gacgtaaaac
    26401 ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg catttccgcc
    26461 cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa gcattgctgg
    26521 agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt
    26581 tctcgatgcc ttcatccagg gtgatgaccg gctgataacc taactcttcc tgcgcacgcg
    26641 taatatccag cgtaaagtca aaattcaact tggagacgcc gtagtgggtc agcggcggct
    26701 cttttgctga cttgcggcct aaacgctcca tgctgcgggc gatcatatcc agcatcgggt
    26761 aagggacgga acgaatacga cagtcaatat tcaactcgtc gatcagcttc tgcacgatgc
    26821 tgcgcagtgt gcgatgctcg ccgttggtga tgttgtacac acgcccggaa ggtagcttat
    26881 cgcaggcttc ctggcttgcc agccacattg cgtgcacggc attttcatag taggtcatat
    26941 cgaccagcgc actgccgcca tgcggtaaca gaatactgcc gtagtggtgc atcatatgcg
    27001 ccagacgggg aataaagact ttatcgtgcg gtccgaacag actttgtggg cgcagaatag
    27061 taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat cacttcttcg ctggctgctt
    27121 tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa atcttcttta atatcgcgat
    27181 ggtggtgata atcgaagtac agggaggggg aagagatatg aataaagtta cgcacacccc
    27241 aggcgacagc ccattcaccc aggcggcgag tggcgcgaac gttagccaga tcgaaagcct
    27301 gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca cagcgtatca atgcccgcga
    27361 gcatcacttt agcttgtgat gaaaccagct cggtcagatc cgtcggaaca aactctgcgc
    27421 ccattttttc cagcaattta cccattgctt cgttgcgacc gctcgcacgc acgctgatgc
    27481 ctttctggca taaaaactct accgcgtttc gacctaagcc gctggtggcg ccggtaacca
    27541 gtaccttcat atcaatccac tgttgttgag aaaatgacgt gcgcattctt ccgtgatttc
    27601 ccccatgatg caatgggaaa catgaaagaa taacgcaggt tttgtcgatt aatctgtgct
    27661 ttgttctgcc agtctggcga tttgttttgc cattccgcga aaaataaaca gatgcgcggg
    27721 gatcatcaat aaccagtaaa acagccccgg cataccgtgc ggatgccaga aagcgcggac
    27781 atcgatagta cgatagtcac ctttatcttc cagggtaaaa cacagtcgtc ccagccccgg
    27841 cgctttcatg ccaaataaca acgtaagttg ctttttcggt tcaacgacaa tcactttcca
    27901 gctatccacc gcatcgccgg tctgtaaata ttcgcgctcc gggcggcctt tcgccagatt
    27961 atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac aaaatattgc caaagaaata
    28021 acgctcttta ccgccgattt ggttcaccac cagccataaa gccgcaaggc tggcggacgt
    28081 tttaacggta aaccccgcct gtttggcgaa ataaccgtac tccggtcgcc agcgggcaaa
    28141 ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt ttttcttcct ctttcaacgt
    28201 gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg atgagtgcac gtagcgcggt
    28261 atcatctgcc agcagatcgt gtttcagccc ctgaatcaac gccctggcgg tggtgggcgg
    28321 tacggaagta atcacattga gaaaccacac cgaaatccag cgggtgggga gggggatggg
    28381 gatcaaccag cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact
    28441 gagcacctct ggtccggcgg cttcgaagat gcggtgttcg ttggctggat ggtctaacag
    28501 cgccaccaga tagtgcagca agttttccag cgcgatgggc gtggtgcgtg aacgtaccca
    28561 gcgtggcggc gttaacactg gcaggttgta gaccatatcg cgcatgactt cgaacgccgc
    28621 tgaacctgcg ccaacgataa ttccggcccg aagttcggtc actggtacat tcgcttcacg
    28681 aagaatgtcc gccgtagcct gacgagcacg cagatgatcc gactgctcat gtggcggggc
    28741 ctgcaacgaa ctgagaaaga ttaattgctt aactggtact tcacgtagcg catcgcggac
    28801 gttgagagcc acttggcgct cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac
    28861 cagaaaatag acggtatcga tatcctgcaa cagggccgga aggttatccg gccagctgag
    28921 atcgacttta tggcaactga cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg
    28981 tgccgccgcc aggatctgat gcccttgctg gctgagtgtg cgcaccagat gctgaccaat
    29041 gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca
    29101 ggaatgcgcg ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg
    29161 tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc
    29221 ctaacgcggc agcattttct tccccgacgc gaacaaacag catattagtg tcctgacgca
    29281 tcacatccgc gcctgcttca cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt
    29341 cctgcaaccg tgcgacgtta tttttcaggg catacatccc ggcggcagcc agaatcctgg
    29401 actgacgcat cccgccaccg gccattttcc gccagcggat ggcacgttta atgtaatcac
    29461 gattaccgac gagtaatgaa ccgactggcg tcccaagacc tttcgacagg caaatggtga
    29521 acgaatcaca atattgcgtg atctctttca gttcgcagcc gtaagccacc acggcattaa
    29581 agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc gcgggtaaat tcccatgctt
    29641 ctttcaggta ttcacgcggc agcactttgc cgttgtgggt gttttccaga ctgagtaatt
    29701 tggtgcgggc gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta
    29761 gcgtgccgtc ggcagccgcg tcgatgggtt gcggctgaat actgcccagc accgctgcgc
    29821 caccggcttc aaacagatag ttatgcgcgg cctgaccgac aatatactct tcgccacgtt
    29881 cgcagtgact gagcagagcg accaagttgg cctgagtgcc ggtaggcaga aaaatggcgg
    29941 cttctttacc ggaaagctct gctgcatagt cctgcagagc attaacggta gggtcgtctc
    30001 cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc gagcatggcg cggctcggtc
    30061 gggtaacggt atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc
    30121 acccttttta ccttagccag ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc
    30181 tgataattgc gcgcaacata tacaggctaa aacctttggc ctgttcgagt ttgatctgcg
    30241 gtggaatggc taattcttct ttggcgacca ccacatccac caacaccgga ccgtcgatag
    30301 agaaggcgcg ttgcagggct tcatcaactt cagaggcttt ttctacacgg atacccgtaa
    30361 tgccgcacgc ttcggcaatg cgggcaaatt tggtgtcgtg cagctcagta ccgtctgtca
    30421 ggtaacctcc ggccttcatc tccatcgcca caaagcccag cacgctgtta ttaaagacga
    30481 taattttcac cggcagtttc atctgcacta ctgagaggaa atcgcccatc aacatgctaa
    30541 aaccgccatc gccgcacatg gcgaccacct gacgttccgg ctcggttgcc tgcgcaccca
    30601 acgcctgcgg catggcgtta gccatcgaac cgtggttaaa cgaacctaac aggcgacgct
    30661 tgccgttcat tttcagataa cgtgccgccc ataccgttgg cgtgccgacg tcacaggtaa
    30721 agatggcgtc atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg
    30781 ctttctcgct cggtttagct aaatcgtcca gccctttgcg ggcatcgcgg taatcttcca
    30841 gcgctttatc cagaaacttg cgatcggttt tttcttccac cagtggcagc aatgcgcgca
    30901 gggttgattt gatatcgccg accagcgcca tatctacctt gctatgcgcg ccgatgctgg
    30961 ctggattgat atcaatctga ataattttgg catcggtcgg gtagaacgca cggtagggga
    31021 actgagtacc gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga
    31081 atccgattaa cccggtcatt ccaacatcat acggattatc gtattcgaca tgctctttac
    31141 cgcgtagggc atgaacaata ggtgctttaa ttttcccggc aaactcaact aactctttat
    31201 gcgcccccgc gcagccgctg ccacacatca gggcgatatt gctggaataa cgcagcagtt
    31261 gcgccagttt gcgtaactct tcttcttccg gcgtcacgac tggctgtggc gcatgatacc
    31321 agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac gtcgcccggt aacacaacaa
    31381 cggaaacgcc acggttaagc accgctttgc gcatagcaat tgccagcact tgtgggatct
    31441 gctccgggct ggaaaccagc tcgcaatagt gactacattc gcggaatagc tcttgtgggt
    31501 gggtttcctg gaaatagccg ctgccaattt cgctggaggg aatatgagcg gcaatcgcca
    31561 gtaccggaac gttattgcgg tggcaatcga ataggccgtt gatcaagtgc aggttgcctg
    31621 ggccgcacga tccggcacag accgccagtt ctcctgaaag ttgtgcttca gcgccagcgg
    31681 caaacgccgc cacttcttca tggcgggtgg acatccactc gatggtgccc atgcgattaa
    31741 gactgtcgct aagaccgttc agagagtcgc ccgtgactcc ccagatgcgt ttcacgcctg
    31801 ccgattcgag tgttttggcg atataagctg caaccgtttg tttcatggtt ctccatctcc
    31861 tgaatgtgat aacggtaaca agtttagttc atctgacgga gggggaaggg atgggagaga
    31921 aaggaggcac taacggttaa atagcccgat gaaaggaata tcatcgggca taaggcgatt
    31981 atgcgagaac caaatccccc tgcggatggc aggagcaggc cagtacgtaa ccttcagcga
    32041 tttcggcgtc ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa accacttttg
    32101 tcttacagca gccgcaaaca cccgcacggc aggcagcgac aaccggaacg ttattgcttt
    32161 ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt
    32221 tggtgaattt cagaccgctg gtcgccgctt ctgctactgg ggtgaagaat ttctctttaa
    32281 agaaacgcgt cacgccgagc gctttcactt cctgctctac ccaatccata tacggagccg
    32341 ggccgcaggt catcacggta cgtgaagcta agtcaggtac acctgccagc agttcgcgag
    32401 tgagacgacc agcgataaag ccttcggtaa cgttattttc tgccaccagc gttaccggat
    32461 agttacgcca ctcatcggcg aaaataacat cctgcggcgt acgcacgttg tagatcaccc
    32521 gcacatcggc ctgtggacgg ttcttcgcaa gccagcgacg catcgacata atcggcgtga
    32581 cgccgcagcc tgccgccagc aacaggaatt tatcttctgc tttatcgtcg caggtaaatt
    32641 cccccatcgc gtccgaaagc cagagataat caccgcgttt tacatcgcgc gtcagccact
    32701 gggagccgac accgtcatca atccgccgca cggtcagggt gatatattca ctcacgcctg
    32761 gcgtggagga aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg
    32821 catattgccc ggcgcgatat gggtagtgat cgtggcaaat cagggaaatc gtccacacat
    32881 ccggcgtttc ttgcgtaatg tgatgaacct gcatccgcca cgggcattga ttcgttggca
    32941 tcgtcatcga caaactcctt acgcgctcaa cagttgcttc atgtcttctt caacagtggt
    33001 gatagaacgc aggccgaatt tctcgttcag caccgccagc aggtctggtg tcaggaaacc
    33061 aggtgcagtc gggccggtga cgatattttt cacgcccaga gaaagcagcg tcagcagaat
    33121 gacaatcgct ttctgttcaa accaggagag caccagcgac agcggcagat cgttcacacc
    33181 acagcccagt ttctctgcca gagtgacagc cagaataatt gctgagtaag catcgttaca
    33241 ttgacctgca tctaccagac gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa
    33301 gcgatattta ccacaggcga gggtcaggat caggcagtca tccggcacgc tggtggcgaa
    33361 atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg
    33421 acgcagtttt tcacggctaa ccagatcaat cagcgtatcg gctgcaccaa gcagcgtctg
    33481 gcgaccaaag cccacggtga tcaggtgagg aatttcgctg tacgggaagc ccgccatttg
    33541 ttgcgcctgg gcgatgaccg cagagaaatc ttcgccatcc agatgacgca cgccaggcca
    33601 gccaacgatg ctgcgggtcc agatacgatc gtcataagcg cctacggttg ggtcgatgat
    33661 gcagttcgag gtcatcacga tggggccagg gaaacgagcg aactccactt gctgattctg
    33721 ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg ggtagccatg
    33781 cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg tctgttccag
    33841 caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac ccgccgtcgc
    33901 tttgacgttg acctgggttg gcgtcgggtg accgtattta ccggtttcgc ctgcatccag
    33961 aatgctcatc actttgaagt tcatctggcc gatttccatt gaacactcaa gaagcgcgtt
    34021 catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg cataaatatc
    34081 gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac ctttcaggcc
    34141 atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt tatctttgtt
    34201 aggggtaaat tctgctgcct gacgttgcag ctcgccgaga tcatcgctca ccagttgcag
    34261 gtcagccatc gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt gtgctttcag
    34321 cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca atacgcggag aatcgaagtt
    34381 aacgttggtc agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat
    34441 gatgccgtat tcacgcgctt ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag
    34501 taaatcctga aggtcagaag tttccgccgt tttaccacac atcccctgcg cgtatgagca
    34561 gccgtttcct gccggagtac ggatagtttg ttcacattgc acacaaaaca tgatcacacc
    34621 ttttaaagtt atatttaata tacatgttta aggttaagac gcttaacgcg gggataaaag
    34681 ggatttttca tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta
    34741 ccgccagaga ggtattacgc agagaaaaag gcgatgagga tcggcactaa caggctaaga
    34801 ataaaaccgt gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg
    34861 ggcagggtga aatccattga tgtggcaccg cataagccca gtgcagtaga gcggctgcgg
    34921 cgaatcagcc cagggatcaa cataatagca atcagttcac gggccagatc attaaaaaac
    34981 gccgcgctcc cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac
    35041 cagccgaaac cggaggccat tgccagcgcg gtattgatgg ggagatcaag aataaaggcg
    35101 ttaattaaac caccaattaa tgaactgaca accaccacca cggcgacaat cattccccgg
    35161 cgattaagga caatctgctt taaggtcatg ccattattgc gcaactgaat accaacgagg
    35221 aaaagtagca aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt
    35281 ccactcagac caatggcaaa accaatcact actacgccgc acagttttag cgactccagc
    35341 gccatcgcaa tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcaggcct
    35401 cgctccagcc acatcagggc ggcaatatta cacagtaaaa taacggtaat actgacggca
    35461 gaataatgca gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata
    35521 cccataaaaa agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac
    35581 gcagcttgtt ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag aatgattaac
    35641 agcccagaaa acatgaaaat ccagtccttg caaagaagaa gtcgaaatgc gcgatgacac
    35701 actactgaaa gcggaaggac gagtaaagtt gcaattaaaa ggaaatgtta tgcatcagag
    35761 ggggattttt aatcctatct gagtgtttag cattgttgcg ctcctttcat gtgcaatgcc
    35821 atataaagcc actggcggag ggagcgcgca gggggcagcc agtcgccgcc gccccc
//
LOCUS       DADLSE010000026        35831 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLSE010000026 DADLSE010000000
VERSION     DADLSE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.026"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..510)
                     /locus_tag="JN692_001288"
     CDS             complement(<1..510)
                     /locus_tag="JN692_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7263644.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMMA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFM"
     gene            667..1707
                     /gene="gpr"
                     /locus_tag="JN692_001289"
     CDS             667..1707
                     /gene="gpr"
                     /locus_tag="JN692_001289"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7263645.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1747..2241)
                     /locus_tag="JN692_001290"
     CDS             complement(1747..2241)
                     /locus_tag="JN692_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7263646.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2432..3316
                     /gene="yghA"
                     /locus_tag="JN692_001291"
     CDS             2432..3316
                     /gene="yghA"
                     /locus_tag="JN692_001291"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7263647.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN692_001292"
     CDS             complement(3588..4013)
                     /gene="exbD"
                     /locus_tag="JN692_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7263648.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(4020..4754)
                     /locus_tag="JN692_001293"
     CDS             complement(4020..4754)
                     /locus_tag="JN692_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7263649.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            5006..6193
                     /gene="metC"
                     /locus_tag="JN692_001294"
     CDS             5006..6193
                     /gene="metC"
                     /locus_tag="JN692_001294"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7263650.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6333..6992
                     /locus_tag="JN692_001295"
     CDS             6333..6992
                     /locus_tag="JN692_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7263651.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(7032..7931)
                     /locus_tag="JN692_001296"
     CDS             complement(7032..7931)
                     /locus_tag="JN692_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7263652.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGYKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8125..9288
                     /gene="yqhD"
                     /locus_tag="JN692_001297"
     CDS             8125..9288
                     /gene="yqhD"
                     /locus_tag="JN692_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7263653.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHFSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9393..10220
                     /gene="dkgA"
                     /locus_tag="JN692_001298"
     CDS             9393..10220
                     /gene="dkgA"
                     /locus_tag="JN692_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7263654.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            10420..11346
                     /locus_tag="JN692_001299"
     CDS             10420..11346
                     /locus_tag="JN692_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7263655.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11397..11654
                     /gene="yqhH"
                     /locus_tag="JN692_001300"
     CDS             11397..11654
                     /gene="yqhH"
                     /locus_tag="JN692_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7263656.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11697..13916)
                     /locus_tag="JN692_001301"
     CDS             complement(11697..13916)
                     /locus_tag="JN692_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7263657.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKVSSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN692_001302"
     CDS             complement(14027..15439)
                     /gene="ftsP"
                     /locus_tag="JN692_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7263658.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN692_001303"
                     /gene_synonym="parF"
     CDS             complement(15514..16251)
                     /gene="plsC"
                     /locus_tag="JN692_001303"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7263659.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN692_001304"
     CDS             complement(16485..18743)
                     /gene="parC"
                     /locus_tag="JN692_001304"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7263660.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAAHG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(19289..19771)
                     /locus_tag="JN692_001305"
     CDS             complement(19289..19771)
                     /locus_tag="JN692_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7263661.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN692_001306"
     CDS             complement(19824..20216)
                     /gene="ygiW"
                     /locus_tag="JN692_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7263662.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            20368..21027
                     /gene="qseB"
                     /locus_tag="JN692_001307"
     CDS             20368..21027
                     /gene="qseB"
                     /locus_tag="JN692_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7263663.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            21024..22373
                     /gene="qseC"
                     /locus_tag="JN692_001308"
     CDS             21024..22373
                     /gene="qseC"
                     /locus_tag="JN692_001308"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7263664.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(22419..22751)
                     /locus_tag="JN692_001309"
     CDS             complement(22419..22751)
                     /locus_tag="JN692_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7263665.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            23070..23651
                     /gene="mdaB"
                     /locus_tag="JN692_001310"
     CDS             23070..23651
                     /gene="mdaB"
                     /locus_tag="JN692_001310"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7263666.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            23682..23996
                     /locus_tag="JN692_001311"
     CDS             23682..23996
                     /locus_tag="JN692_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7263667.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN692_001312"
     CDS             complement(24044..25936)
                     /gene="parE"
                     /locus_tag="JN692_001312"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7263668.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPCFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAALVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN692_001313"
     CDS             complement(25965..26546)
                     /gene="yqiA"
                     /locus_tag="JN692_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7263669.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQVVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN692_001314"
     CDS             complement(26546..27373)
                     /gene="cpdA"
                     /locus_tag="JN692_001314"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7263670.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(27398..27820)
                     /locus_tag="JN692_001315"
     CDS             complement(27398..27820)
                     /locus_tag="JN692_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HAY7263671.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN692_001316"
     CDS             complement(27821..28450)
                     /gene="nudF"
                     /locus_tag="JN692_001316"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7263672.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            28655..30136
                     /gene="tolC"
                     /locus_tag="JN692_001317"
     CDS             28655..30136
                     /gene="tolC"
                     /locus_tag="JN692_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7263673.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQMKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            30284..30955
                     /locus_tag="JN692_001318"
     CDS             30284..30955
                     /locus_tag="JN692_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7263674.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            30961..32121
                     /locus_tag="JN692_001319"
     CDS             30961..32121
                     /locus_tag="JN692_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7263675.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTNLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN692_001320"
     CDS             complement(32159..32974)
                     /gene="ygiD"
                     /locus_tag="JN692_001320"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7263676.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADITMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            33090..33863
                     /gene="zupT"
                     /locus_tag="JN692_001321"
     CDS             33090..33863
                     /gene="zupT"
                     /locus_tag="JN692_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7263677.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(33921..34091)
                     /locus_tag="JN692_001322"
     CDS             complement(33921..34091)
                     /locus_tag="JN692_001322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263678.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN692_001323"
     CDS             complement(34352..35005)
                     /gene="ribB"
                     /locus_tag="JN692_001323"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7263679.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(35109..35257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35319..35669
                     /gene="ubiK"
                     /locus_tag="JN692_001324"
     CDS             35319..35669
                     /gene="ubiK"
                     /locus_tag="JN692_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7263680.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
BASE COUNT         9011 a         9450 c         8903 g         8467 t
ORIGIN      
        1 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
       61 cagctcacga cctttatcat catttcccgg ataacctcca acggaactta atccgtcagg
      121 tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt
      181 cagtccacga ttctcatgca ccaccaccac ggctggcgtt ttgccgctca tttttgcggg
      241 tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc
      301 agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag
      361 caaatcaaac aacgctaatg ccatcatccc gcccaccgca tacttcgccg caaggttgag
      421 gaactcacgt ttcgaaattt tcccgtgagc gtaatagtcg tagtaatcca acaactcttg
      481 tgggaaatct ttggcggtta aacgcggcat cgttacattc ctcaactggt gtttttttaa
      541 gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg
      601 atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga
      661 gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc
      721 ggaaaaagtg gtttacgcct gcccgcgtta tcgctcggtt tatggcacaa tttcggtcac
      781 gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg
      841 cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt
      901 cgcctgctgc gcgaggattt tgccgcttat cgcgatgaac tgattatctc taccaaggct
      961 ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata cctgctcgcc
     1021 agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat
     1081 cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa
     1141 agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg
     1201 gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta
     1261 ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc
     1321 tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt
     1381 ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg
     1441 cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga
     1501 caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta
     1561 ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg
     1621 acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat
     1681 ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga
     1741 atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag
     1801 cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc
     1861 atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt
     1921 gtttttcagc gacgttgcgt ccattttccc cagccagttc agcttctctt tgttctcgga
     1981 gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc
     2041 gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat
     2101 cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac
     2161 taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc
     2221 attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc
     2281 gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata
     2341 attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt
     2401 aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca
     2461 gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat
     2521 gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg
     2581 taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc
     2641 gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga
     2701 tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag
     2761 cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga
     2821 tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag
     2881 cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga
     2941 agcgatcccc ctgctaccga aaggtgcaag tatcatcacc acttcgtcaa tccaggcata
     3001 ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag
     3061 ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg
     3121 ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt
     3181 tggtcagaaa acgcctatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt
     3241 ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg
     3301 cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt
     3361 cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga agattcaaca
     3421 tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg
     3481 ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca
     3541 atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg
     3601 gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt
     3661 accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct
     3721 ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg
     3781 ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc
     3841 ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt
     3901 gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg
     3961 atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat
     4021 tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga
     4081 tccaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta
     4141 aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg
     4201 aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta
     4261 gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac
     4321 agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt
     4381 tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct
     4441 tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc
     4501 aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt
     4561 gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg
     4621 ctcttactga agaagattgc ccaggtgact acggaggcca aaataagccc aatcatcacg
     4681 cacttaacga caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatt
     4741 aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat
     4801 caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc
     4861 tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt
     4921 ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca
     4981 gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa
     5041 tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc agcgcgcttc
     5101 ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg
     5161 cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg
     5221 tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa
     5281 ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga
     5341 accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt
     5401 tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt
     5461 tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc
     5521 cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt
     5581 gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca ccaaatatct
     5641 ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca
     5701 gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac
     5761 cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa
     5821 agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc
     5881 tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc
     5941 ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag
     6001 tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc
     6061 agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg
     6121 cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc
     6181 acggattgta taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc
     6241 ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat
     6301 aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg
     6361 cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact
     6421 ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag
     6481 gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc
     6541 ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg
     6601 ggcgctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat
     6661 atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac
     6721 gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa
     6781 accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca
     6841 gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga
     6901 cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg
     6961 tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac
     7021 cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc
     7081 acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc
     7141 atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga
     7201 cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg
     7261 aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg
     7321 tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg
     7381 accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg
     7441 atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc
     7501 tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc
     7561 gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata
     7621 tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca
     7681 aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca
     7741 ttgatataac cgattttata cccggaaaag agaattatga tgccaggctc gtacatcacc
     7801 ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac
     7861 aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg acaaatctct
     7921 tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt
     7981 tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga
     8041 gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata
     8101 ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt
     8161 ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta
     8221 ttgattacct acggtggcgg cagcgtgaaa aaaaccggtg ttctcgatca agttctggat
     8281 gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa
     8341 acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt
     8401 ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa
     8461 aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg
     8521 atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc
     8581 tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca gccggtattt
     8641 gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta
     8701 gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt
     8761 caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg
     8821 aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg
     8881 aacggtttga ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa
     8941 ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg
     9001 tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg
     9061 aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat
     9121 ttctttgagc aattaggcgt gccgacccac ttctccgact acggtctgga cggcagctcc
     9181 atcccggctt tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat
     9241 gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc
     9301 tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc
     9361 tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac
     9421 aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag
     9481 taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg
     9541 cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag
     9601 aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc
     9661 tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg
     9721 ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg
     9781 gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg
     9841 atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac
     9901 gccagctaca cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag
     9961 cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg
    10021 gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc
    10081 cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg
    10141 acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc
    10201 ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg
    10261 acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag
    10321 gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat
    10381 ctatatctgg gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt
    10441 ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa
    10501 acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta tttcggtgaa
    10561 accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat
    10621 cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat
    10681 tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat
    10741 gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca gacactgaaa
    10801 atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca tggcagcgtc
    10861 ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa
    10921 gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta tagctggccg
    10981 tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc
    11041 ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac
    11101 aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg
    11161 gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaat
    11221 gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc tgattttatc
    11281 cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag
    11341 caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga
    11401 aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca
    11461 atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc
    11521 ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta
    11581 acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc tgcttgcgta
    11641 tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa
    11701 cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta
    11761 gacgacactt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta
    11821 gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt
    11881 ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc
    11941 agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc
    12001 aaacgacgct gtttgtcgcc cttcggtacg aagacgtctt cgctcttata accaatcttc
    12061 gccagcgggt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag
    12121 aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc
    12181 atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct
    12241 ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg
    12301 cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga
    12361 taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg
    12421 tcataacgta cgcctgaggc gatgaggatt tttttaatgc ctttcagatc acgcgcacgg
    12481 cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa
    12541 acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac
    12601 atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg
    12661 cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct
    12721 tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc
    12781 gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg
    12841 cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc
    12901 gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg
    12961 ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc gcccttcact
    13021 ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg cggtggctgc
    13081 acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc cgcacacggc
    13141 aaatcttcgc catacggatg cgggattggg tcgattttcc ctggggtatc aagacgggtg
    13201 gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt attacgcaca
    13261 tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc caccagcgga
    13321 cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc
    13381 acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc aataccgccg
    13441 aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac cagcgtggcg
    13501 cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg acgtaaacgg
    13561 cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac accaaaaaac
    13621 agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg
    13681 atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat cccgaagctt
    13741 gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt
    13801 tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag
    13861 tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg
    13921 cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga
    13981 aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat
    14041 tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt
    14101 agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt
    14161 caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt
    14221 tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg
    14281 tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct
    14341 gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt ggcgaaccag
    14401 ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc
    14461 cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc tcaaagaagc
    14521 cgcgaatacg atcaacaatg ctcgccgctt cgccacaggt gatcgacact tcatcgccgt
    14581 tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg
    14641 atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc
    14701 tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc
    14761 cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa
    14821 agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct
    14881 ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc gacttgctga
    14941 cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt
    15001 tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg
    15061 cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg cctggtacct
    15121 gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa
    15181 catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga
    15241 ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg
    15301 gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg
    15361 cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact
    15421 gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg
    15481 aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc
    15541 tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc
    15601 cagctcacga acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac
    15661 aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca
    15721 cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg
    15781 tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg
    15841 ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct
    15901 gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa
    15961 gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc
    16021 atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg
    16081 tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa
    16141 ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca
    16201 gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc
    16261 tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa
    16321 gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg
    16381 gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc
    16441 ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc ttcgctatca
    16501 ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg caaaccgcgc
    16561 atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc cggacgcagt
    16621 ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag aacgtacagt
    16681 tgcgccagac catcctcgcc atgcgcggct tctgccgatg gaatgttgat aatcttgttg
    16741 cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat acggcctgcc
    16801 tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg cataacatgg
    16861 gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgccac cagatcgtta
    16921 aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg atcgtcgctt
    16981 tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt
    17041 tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct acgaccggtg
    17101 gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt gaagctatca
    17161 cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct gcgtacccag
    17221 cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc
    17281 gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg
    17341 tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc caaaatgccc
    17401 tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt catctcttcc
    17461 agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg
    17521 ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat ctcaatcact
    17581 tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg cttgaggact
    17641 ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac cagccattcg
    17701 gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat catattgagg
    17761 ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat cacctgatcc
    17821 atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc gtggtcagat
    17881 tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc agcaatttgc
    17941 tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac cgcgccatct
    18001 tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta gattttacga
    18061 atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc ctgcacgata
    18121 tccagcagct gatcgagcgt ggttttcggc tggtcgatta atgcgattgc cgcctgagct
    18181 acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat accggtggtg
    18241 ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg caaagtgccg
    18301 tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag cagctcggaa
    18361 tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg atcgtccgga
    18421 gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg ctgcgccatc
    18481 aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca
    18541 ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag ttcagacatc
    18601 gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg caacgcacgg
    18661 tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc atgtagcgca
    18721 aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc agccttcaaa
    18781 ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg agatcagcgt
    18841 gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc ttgatataca
    18901 actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc catgccacac
    18961 cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc gagtccaggg
    19021 aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt ttagctgtgt
    19081 ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat acccccgaga
    19141 cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt accggagaaa
    19201 ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc gcgagtgatt
    19261 tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca tccgtttcca
    19321 gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac
    19381 gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa
    19441 tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat
    19501 tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc
    19561 gaaacgcttc cggtgcggtg gtggcagggt cgtcccaggc gacgccaaaa gtctggcttt
    19621 ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt
    19681 tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga
    19741 taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga
    19801 acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt
    19861 tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc
    19921 accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc gctggcatct
    19981 ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag
    20041 gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctgg
    20101 aatcctccgg cctgactttg cgttgccgat gggccagaaa aaccgccctg ctctgctgcc
    20161 atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt
    20221 attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta
    20281 agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc
    20341 aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg
    20401 attggcgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga ctggtttaca
    20461 caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat
    20521 ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag
    20581 cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg
    20641 cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg
    20701 ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac
    20761 gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa
    20821 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
    20881 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
    20941 gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt
    21001 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc
    21061 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
    21121 ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca
    21181 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
    21241 caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc
    21301 acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc tatagctatc
    21361 aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg cgttttgtct
    21421 ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg
    21481 aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc gcactgccga
    21541 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
    21601 cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac
    21661 ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga
    21721 tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt agcccgttaa
    21781 cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga
    21841 aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac
    21901 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
    21961 ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa
    22021 ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa ccgctattgc
    22081 taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ctacagtcca cagggcagcg
    22141 tgatagacgt cacgctgaat gctgataatt tcatcgtgag ggataacggc cccggtgtga
    22201 caccagaggc actggcgcga attggcgaac gcttctatcg cccacccgga caaaccgcta
    22261 ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc caagctgcat gacatgaatg
    22321 ttgagtttgg gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg taacttctct
    22381 gtctatgaga gccgttaaaa cgactctcat agattttact aatagcaaaa tataaaccgt
    22441 ccccaaaaaa gccaccaacc acaaaccaga caaataacga gcgagaatac atatagccaa
    22501 aactcgaaca tttcccgcat aaagagtttt cttaagataa gaataataag tggcgtaaga
    22561 agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata
    22621 ctgcaaatat tgcctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagaa
    22681 tctattaacc aataagtaag cattatatat agcaatgctt caaatattgt tttaattttc
    22741 tgctttaaca tatatcagta cgctcataaa aatccattta taattatcaa catgacacgt
    22801 tgatattttc atcttttact tattcaaagc catattttca atccccaaaa aaaccgaacc
    22861 aaacgtgtaa attattccgg tgcgagtaag aattatgagg aatggctatc agtattgtca
    22921 ttttcagaaa atatttatcc tgcatcggtg agtcagagct agatcagact tttgctaaat
    22981 tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc gctctgtgac gtactatagt
    23041 cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat cctgattatc aacggcgcga
    23101 aaaaattcgc ccactccaat ggtcaactga acgacaccct gaccgaagtc gcggatggca
    23161 cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc cgacagcgac tacaatgtca
    23221 aagcggaagt acaaaacttt ctctgggctg atgtggtgat ctggcagatg ccaggctggt
    23281 ggatgggcgc gccgtggaca gtgaaaaaat atattgatga tgtattcacc gaaggtcacg
    23341 ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc gtcgaaaaaa tacggttccg
    23401 gcggcctggt acagggcaaa aaatatatgc tttctctaac ctggaacgca ccaatggaag
    23461 ccttcaccga aaaagatcag ttcttccacg gcgttggcgt tgacggtgtg tatctgccgt
    23521 tccataaagc aaaccaattc ctcggtatgg aaccgctgcc gacatttatc gctaatgacg
    23581 tgataaaaat gcctgatgtt ccccgctata ctgaagaata tcgcaagcat cttgtggaaa
    23641 tttttggtta actagagctc aggctttaga aggagttaac catgcttacc gtaatcgcag
    23701 aaatccgtac tcgtcctggt caacatcacc gtcaggcggt attggatcag tttgctaaaa
    23761 tcgttccaac cgtactgaaa gaagaaggtt gccacggcta tgcgccaatg gtggattgcg
    23821 ctgctggcgt gagtttccag tctatggcac cggattctat cgtgatgatt gagcagtggg
    23881 aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca catgaaggcg tacagcgaag
    23941 ccgtaaaagg cgacgtgctg gagatgaata tccgtattct gcagccaggg atttaatcct
    24001 gccttgtttg cccggccatc ctgaccgggc aatgttcttt cctttaaacc tcaatctccg
    24061 ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc cgagcgtttc ttcgccagca
    24121 gcatatccat catcgcgtca gtacgctgat cgtcttcatc atcgatagtc aactgcacca
    24181 gacggcgagt gttcggatca agcgtggttt cacgcaattg catcgggttc atttccccca
    24241 gacctttaaa acgctggacg ttcggcttgc ctttcttgcg ttttaattgc tcaagtacgc
    24301 cctctttctc ttcttccgtc agcgcgtaat aaacctcttt cccgagatca atacggtaga
    24361 gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa cgcgcggaag tgttttacga
    24421 acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga gtccgcatcc gcgaggatac
    24481 agattttgcc ataacgaagc tggctcagat cgtcgctgtc aggatcgata ccgatcgcta
    24541 ccgaaatatc atgcacttcc tgcgaggcca gcacttcgtc agaagaaact tcccaggtgt
    24601 taaggatctt acctttcagc ggcatgatcg cctgatattc acgatcgcgc gcctgcttgg
    24661 cagatccgcc tgcggagtca ccttccacga ggaacagttc ggtacggtta aggtcctgcg
    24721 cggtacaatc agccagtttg ccaggcagcg ccgggccgct ggtcagcttt ttacgcacca
    24781 cttttttagc cgcacgcata cgacgctggg cgctggaaat cgccatctct gccagcaact
    24841 ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc atctttcacc acgcccgaaa
    24901 ctaatgccgc acactgacgc gaagagagac gctctttggt ctgcccggca aactgcggat
    24961 cctgcatttt aaccgacaga acataggcgc agcgatccca gatatcttcc gccgacagct
    25021 tcacgccgcg cggcagaatg ttgcggtatt cacagaactc acgcatcgcg tccaacaggc
    25081 cctgacgcag accattaaca tgggtaccgc cctgcatcgt tgggataagg ttgacgtagc
    25141 tttcggtcag cagttcaccg ccttccggca gccacagtag cgcccagtcc acagcttcag
    25201 tatcgccagc gaaattaccg ataaacggtt tttctggcag cgtcggtaaa ccgtttaccg
    25261 cttccgccag gtaatcattc agaccgtcct gatagcacca gcgctgttcg gtgttgttga
    25321 tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac tgctttggcc ttcagcacat
    25381 gcgtcaggcg tgaaacagaa aaacacgggc tgtcaaagaa ggtttcatcc ggccagaagt
    25441 gcacgctggt accggtattg cgtttaccgc aagtgccgac aacctgtaaa tcctgcacct
    25501 tttcgccatt ttcaaaggcg atgttataaa cctgaccatc gcggcgcacg ttaacttcta
    25561 cgcgcttcga caaggcgtta actaccgaaa tccccacgcc atgcaggccg ccagagaact
    25621 ggtaattttt gttagagaat ttaccgcctg catgcagacg gcaaagaatc agttcaaccg
    25681 ccggtacacc ttcttccggg tgaatatcca ccggcatccc gcgcccatcg tcaatcactt
    25741 ctaacgactg gtcagcatgt aagattacat ccacgcgttt tgcgtgaccc gccagtgctt
    25801 catcgacact gttatcaata acttcttgcc ccaaatggtt agggcgagtg gtatcggtat
    25861 acatccccgg acggcggcga accggctcaa gcccggtgag tacctcaatg gcatcagcgt
    25921 tataagtttg cgtcatggtt taagttagca attcgagttg atcgtcagag atggtgcaga
    25981 ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc ccgtgaatgc gtggttgccg
    26041 ccttctatga cagtctggcg gcaggaagcg tagtacgcca ccacctggcg gtaatccagc
    26101 acttcatctc ccgtctgttg cagcagccag atcaaatccg gcgcttccag cgggtcaatc
    26161 tgcatgactt tgagatcgta aatatggcgt gactctagca catattgctg cccggtgtag
    26221 gggttctcgt tctgaccgag atagtccgtc agcaattcaa acgggcgtac cgccgggttt
    26281 accaccactg cgggcagcat aaaacattgt gacaaccagg tggcgtaata tccccccagt
    26341 gacgaaccga caatacccag cgaatcgccg ccatgttcca ggacaatgga ttccagcagt
    26401 tctgccgcgt cggaaggata cggcggcaac tgcggaatga tcatctcaac gtcagggtga
    26461 tgttccgcca gccagttttt taacaagctc gcttttgcag agcgcggcga gctgttgaaa
    26521 ccgtgtaaat aaagaagcgt agacatcagt agccttctga agcggtatca ggttggaaac
    26581 gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc atcagcatgt aactcgagag
    26641 tacgccagcc gggcgcgata gtatccagcg taaagttgga acagtgcggt ttaaactgca
    26701 cacaggtcga cggcgttgcc agcaggcggc gaccgttcca gtcgagatcc agctcctgat
    26761 gaatatgacc gcacagcaag tatttgacgt gcggaaactt cgccagcacg gtatccagtt
    26821 cgcccgcgtt acgcagactg tgttgatcga gccaactaca acccgcaggt agcggatgat
    26881 gatgcagcag cagcaacgta tggcgttctg gcgcatcggc cagtttacgt tccagccact
    26941 caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa cacctggcta tccagcaaca
    27001 ggatttgcca ttgctcacca ataaacaccc gcttcgccgg ggagataccc gcatcctgta
    27061 acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg cagccagacg cagggcgcac
    27121 gaaaacttgc gatgccttca gcgaaatgct gataggccgc agaggattga tcctgcgcta
    27181 aatcacctgt cgcgacaatc aggtcgaatt cgtgctggtg tggacgaatc gcctccagca
    27241 ccgcctggta gctttcccag gtgtttaccc ctaacagggc ttcgtgcttt tgtgcaaaca
    27301 ggtgagtatc ggtaatttgt aaaatccttc ctctggcctc accagccaga ggaagggtta
    27361 acaggctttc caaatggtgt ccttaggttt cacgacgcta gtaaaccgga atcgccatcg
    27421 ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa ctggttaatt tgatgctttt
    27481 cgtcgcgttg atgcaacttt ttattaggat aatcataccg cgctttgaag cgaaaaatct
    27541 gctggcttga acacacttca gccaccatcg cgtcatgata cagacgcacc gtcattgacg
    27601 gaaggctcca gtaactgatc gcgggcgcag tctgttctat tgtcaccagg gtagtgtatc
    27661 gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc cacctgatag cttacagttt
    27721 cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg tgaaaagttc atctcgcaca
    27781 ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat ttatgcccac tcatttttta
    27841 acgcttgatg atgcagctgc agccattgca aagcgatgac cgacgctgcg ttgtcgattt
    27901 tcccctcttc tacccactgg tatgcctgtt cccggcttac cacatgaacg cgaatatctt
    27961 cgttttcatc agccagacca tgaataccgc ttgcggtcgt ggcgtccact tcgcccacca
    28021 taattgacga acgctcactg gtgccccccg ggcttgccag gaaacttaac accggtttgg
    28081 tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg acgggcaaca tcttccacac
    28141 tttcaccctc ttcaatcatc ccggcaacca tctccagtag ccagggggtt tcgctggtgt
    28201 cgtacgcggc aatccgaatc tgctcaatca gcacaacttc atcacgcact gggtcaaagg
    28261 gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg ccgtacctca tgactcattt
    28321 gcccgttgaa tagacgatga cgaaatctat aaagatctaa tgaaaaaaag ccgcgataaa
    28381 gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa tgtaacgggc aggttgtctg
    28441 gcttaagcat tgttaatgtc ctggcactaa tagtgaatta aatgtgaatt tcagcgacgt
    28501 ttgactgccg tttgagcagt catatgttaa attgaggcac attaacgccc tatggcacgt
    28561 aacgccaacc ttttgcggta gcggcttctg ctagaatccg caataatttt acagtttgat
    28621 cgcgctaaat actgcttcac cacaaggaat gcaaatgaag aaattgctcc ccattcttat
    28681 cggcctgagc ctttctgggt tcagttcgtt gagccaggcc gagaacctga tgcaagttta
    28741 tcagcaagca cgccttagta acccggaatt gcgtaagtct gccgccgatc gtgatgctgc
    28801 ctttgaaaaa attaatgaag cgcgcagtcc attactgcca cagctaggtt taggtgcaga
    28861 ttacacctat agcaacggct accgcgacgc gaacggcatc aactcgaacg cgaccagtgc
    28921 gtccctgcag ttaactcaat ccatttttga tatgtcgaaa tggcgtgcgt taacgctgca
    28981 ggaaaaagca gcagggattc aggacgtcac atatcagacc gatcagcaaa ccttgatcct
    29041 caacaccgcg accgcttatt tcaacgtgtt gaatgctatt gacgttcttt cctatacaca
    29101 ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc acccaacgtt ttaacgtggg
    29161 cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag tacgataccg tgctggcgaa
    29221 cgaagtgacc gcacgtaata accttgataa cgcggtagag cagctgcgcc agatcaccgg
    29281 taactactat ccggaactgg cggcgctgaa tgtcgaaaac tttaaaaccg acaaaccaca
    29341 gccggttaac gcgctgctga aagaagccga aaaacgcaac ctgtcgctgt tacaggcacg
    29401 cttgagccag gacctggcgc gcgagcaaat tcgccaggcg caggatggtc acttaccgac
    29461 tctggattta acggcttcta ccgggatttc tgacacctct tatagcggtt cgaaaacccg
    29521 tggtgccgct ggtacccagt atgacgatag caatatgggc cagaacaaag ttggcctgag
    29581 cttctcgctg ccgatttatc agggcggaat ggttaactcg cagatgaaac aggcacagta
    29641 caactttgtt ggtgccagtg agcaactgga aagcgcacat cgtagcgtcg tgcaaaccgt
    29701 acgttcctcc ttcaacaaca ttaatgcttc tatcagtagt attaacgcct acaaacaagc
    29761 cgtagtttcc gcgcaaagct cattagacgc gatggaagcg ggctactcgg tcggtacgcg
    29821 taccattgtt gatgtgttgg atgcgaccac cacgctgtac aacgccaagc aagagctggc
    29881 gaatgcgcgt tataactacc tgattaatca gctgaatatt aagtcagccc tgggtacgtt
    29941 gaacgagcag gatttgctgg cactgaacaa tgcgctgagc aaaccggttt ccactaatcc
    30001 ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt gctgatggtt atgcgcctga
    30061 tagcccggca cccgtcgttc agcaaacatc cgcacgcact accaccagta acggtcataa
    30121 ccctttccgt aactgatgac gacgacgggg cttcggcccc gtctgaacgt aaggcaacgt
    30181 aaagatacgg gttatctgcc gcattcttcc cccttctcgc ttcaatttcg accagccatc
    30241 ctctattctg atgggtattt accactggtc ccggaagaca aaaatgaaac ggacaaaatc
    30301 catacgccac gcatcgttcc gcaaaaactg gagcgcacgc catctgacac cagtcgctct
    30361 cgcggttgcc actgttttta tgctggctgg ctgtgaaaag agtgatgaaa cagtgtctct
    30421 ctatcaaaat gctgacgact gttcagctgc aaacccaggc aaaagcgccg aatgtaccac
    30481 cgcgtacaac aatgcgctga aagaagccga acgtactgcg ccgaaatacg ccacccgtga
    30541 agactgtgtt gctgaatttg gtgaaggtca gtgtcagcaa gcaccagccc aggctggcat
    30601 ggcaccagaa aaccaggcgc aggctcagca atccagcggg agtttctgga tgccgctgat
    30661 ggccggttac atgatggggc gtctgatggg cggcggcgcg ggatttgcac agcagccgct
    30721 gttctcctcg aaaaacccgg ccagtccggc ttacggtaaa tataccgacg cgacgggtaa
    30781 aaactatggc gcagcccagc caggccgcac catgaccgta ccgaagacgg caatggcacc
    30841 aaaaccggcg accaccacta ccgttacccg tggcggtttt ggtgaatctg ttgccaaaca
    30901 aagcactatg cagcgtagcg caaccggcac ctcttctcgt tcaatgggtg gctgataccg
    30961 atggaaagag tcagtattac cgagcgcccg gactggcgtg agaaagccca cgaatacggt
    31021 ttcaattttc acaccatgta cggcgagccg tactggtgtg aagatgctta ctacaagttg
    31081 accctcgccc aggttgaaaa gctggaagaa gtcaccgccg aactgcacca gatgtgcctg
    31141 aaagtggtgg aaaaagtgat cgccagcgat gagctgatga ccaaattccg cataccaaaa
    31201 cacacctgga gttttgtgcg tcaatcatgg ctgactcacc agccatcgct ttattcgcgt
    31261 cttgatctgg cgtgggatgg cactggcgaa cctaaacttc tggaaaataa cgccgatacg
    31321 ccaacgtcac tatacgaggc ggcgttcttt cagtggatct ggctggaaga tcagcttaac
    31381 gccggtaact tgccggaggg cagcgaccag tttaacagtc tgcaagaaaa actgatcgat
    31441 cgcttcgttg agctgcgtga acagtatggc ttccagttgc tgcatctcac ctgctgtcgc
    31501 gacacggtgg aagatcgcgg aaccattcag tatttgcagg actgcgcaac cgaagctgaa
    31561 atcgccaccg agttcctcta catcgacgag ataggtttag gcgaaaaagg ccagttcacg
    31621 aatttacagg atcaggtgat ttctaacctg ttcaagcttt atccgtggga atttatgctg
    31681 cgcgaaatgt tctccaccaa gctggaagat gctggcgtac gctggctgga accggcgtgg
    31741 aagagcatta tctccaacaa ggcacttcta ccgctactgt gggagatgtt cccgaatcac
    31801 ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc cgcaaatgga aaaatatgtg
    31861 gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga tcattgagaa cggcaaaacc
    31921 attgaagcag cggaaggtcc gtatggcgaa gaagggatga ttgttcagca attccacccg
    31981 ttaccgaaat tcggcgacag ctatatgctg attggtagct ggctggtgaa cgatcaacct
    32041 gccggaattg gcattcgtga agaccgtgcg ttgatcaccc aagatatgtc tcggttttat
    32101 ccacatattt ttgttgaata agccacgata ccggatggca ctcgccatcc ggtaattgtt
    32161 agcctatctg caccgacagc atacttaaac tgcccatttc tataccatcg accggaatgg
    32221 taattggctc ctgcccatcc cacacaccta acacatacaa caacggcaga tagtgctctg
    32281 gcgttgggtt cgataacgcg ccaccttcat ggtcgagata attcaccaga ggatgctgct
    32341 ctaccggccc ttgccacgtc agattcgctt tcacatactc attaaacgac gtcgcccacg
    32401 gatacggtga actatcaccg tgccacttca ctgtgcgcag gttatgcacc acgttaccgc
    32461 tggcgaccaa cattattcct tcatcacgca gcgccgcgag tttgcgcccc atttcgaaat
    32521 gccaggcggc aggtttgcta ctgtcgatac tcaactgcac catagtgata tcggcgtcag
    32581 gatacatctt aatcagcacg ccccacgagc cgtggtcaaa gccccaggct tctttatcca
    32641 gcgtcaccgg gatcggcgct aacagctcaa ccagacgctg tgccagcgca ggcgaacccg
    32701 gagcaggata atgcgtatcg tacagcgcct gcgggaagcc accaaagtca tgaatcgtgg
    32761 gcggcgtctc catcgcggtc actcctgttc cacgggtaaa ccagtgagcc gaaaccacca
    32821 caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg ccagctgcgg gtatacaaat
    32881 tatcttccag cacgttcatc ggactaccgt gacctaaaaa caatgctggc atacgtgttg
    32941 aagacatgat gatatcctta actaaaggtg tcattttgat atcctcacaa tacgcttgtt
    33001 cggcggagta agaacccgga taacaatgat gatgatcatc agttattttg acgatctgcc
    33061 tgaaggtgaa gatttataag gagttgtcga tgtcagtacc tctcattctg accatactgg
    33121 cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct cggacaaaaa ccctcgaacc
    33181 gcttactggc gttttcgcta ggttttgcgg cggggatcat gttgctcatc tcattaatgg
    33241 aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc ggtgttgggt tatggaatgt
    33301 ttatcttcgg tctgcttggc tactttggcc tggaccgcat gttgccgcat gcgcatccgc
    33361 aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc gatcaagcgc acagccattc
    33421 tgctcactct cggcatcagt ctgcataact tcccggaagg gattgccacc tttgtcacgg
    33481 cgagcagcaa cctggagctg ggatttggca tcgcactggc cgtcgcgttg cacaatatcc
    33541 ctgaaggtct ggcagtggca ggcccggttt atgcggcaac ggggtctaaa cgcaccgcga
    33601 ttctgtgggc ggggatttct ggactagcag aaattcttgg tggtgtgctg gcgtggttaa
    33661 tcctcggtag catgatttcc ccggtggtca tggcggcaat catggcggcg gttgcaggaa
    33721 ttatggtggc gctctcggtt gatgaattaa tgccgctcgc caaagagatt gaccctaata
    33781 ataaccccag ctatggcgta ctatgtggaa tgtcagtgat gggattcagt ttagtgctgc
    33841 tacaaacggc gggaattggt taaaaacaac atcagaaatt gtccagcaca tggacaactt
    33901 ctgccagctt cacgaaagcc ttattctacc ttttcggctg tctcttctct cgtactgttt
    33961 aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct ggcgaaacgc ccgacaataa
    34021 cgtttcaaat gcaggaaata acccaccaca accagagcaa tcaatacaat ccagtaccag
    34081 gcgataaaca tccttttacc cttaatattt aacaatacta aacatttagc gcataaattt
    34141 cacatatcct ttttcggata tattcatatg gtcgcataat aacggaacaa ttatatgatt
    34201 ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga aagagaagcg
    34261 gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca acttcggttt
    34321 cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca tgtgcctgac
    34381 ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg gcaaactcaa
    34441 tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac agcacaccag
    34501 ccggtttaaa gccggccagc gtcatcaaat caatagtcgc ttcagtatga ccgccacgcg
    34561 tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga ttcagatcag
    34621 aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca gcggcagaaa
    34681 caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg ccataggcgc
    34741 tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct tcagtgatgc
    34801 acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca acggtcatgg
    34861 tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca agcaccatta
    34921 caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa ggcgtaccaa
    34981 aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat ggttaccaga
    35041 atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg gtccatgccc
    35101 gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa ttacaccgga
    35161 tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga gttgatttac
    35221 cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa cgctaatgat
    35281 tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct tatcgcacta
    35341 caatggtact caacacctat cagtacaggg aaactacaat gattgacccg aaaaaaattg
    35401 agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag ttcggggaag
    35461 atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc gaccttgtaa
    35521 gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag aaactggcgc
    35581 tgcttgaaca aagaatcagc gaactggaaa atcgcagtac tgagatcaaa aagcagccag
    35641 atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg taaattatga
    35701 attaatcctc taatttacag tgttttctat tacattatcg cttaatccca ttcagagtat
    35761 catcacaaaa atgaatgatt gcaattgaaa atataattgt tatgtattct ttaagtttta
    35821 cttaatcgat a
//
LOCUS       DADLSE010000027        35151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLSE010000027 DADLSE010000000
VERSION     DADLSE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.027"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1582
                     /locus_tag="JN692_001325"
     CDS             <1..1582
                     /locus_tag="JN692_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7263681.1"
                     /translation="LAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAA
                     GRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGAISVD
                     ATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNK
                     DNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSS
                     KDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQIYMS
                     NSTTFDDQGFASNNTGLSGPVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVN
                     GSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTT
                     NRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKP
                     WFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLS
                     CTITFGKEIDESRNYICQ"
     gene            1598..2631
                     /locus_tag="JN692_001326"
                     /pseudo
     CDS             1598..2631
                     /locus_tag="JN692_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(2713..3051)
                     /gene="rcnB"
                     /locus_tag="JN692_001327"
     CDS             complement(2713..3051)
                     /gene="rcnB"
                     /locus_tag="JN692_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7263682.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3270..4118)
                     /locus_tag="JN692_001328"
     CDS             complement(3270..4118)
                     /locus_tag="JN692_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7263683.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARDHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLFTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            4240..4512
                     /gene="rcnR"
                     /locus_tag="JN692_001329"
     CDS             4240..4512
                     /gene="rcnR"
                     /locus_tag="JN692_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7263684.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      4604..4700
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4735..5524
                     /gene="thiM"
                     /locus_tag="JN692_001330"
                     /pseudo
     CDS             4735..5524
                     /gene="thiM"
                     /locus_tag="JN692_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
     gene            5521..6321
                     /gene="thiD"
                     /locus_tag="JN692_001331"
     CDS             5521..6321
                     /gene="thiD"
                     /locus_tag="JN692_001331"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7263685.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6386..7204
                     /locus_tag="JN692_001332"
     CDS             6386..7204
                     /locus_tag="JN692_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7263686.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            7256..8002
                     /locus_tag="JN692_001333"
     CDS             7256..8002
                     /locus_tag="JN692_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7263687.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(7976..8941)
                     /locus_tag="JN692_001334"
     CDS             complement(7976..8941)
                     /locus_tag="JN692_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7263688.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(8938..9941)
                     /locus_tag="JN692_001335"
                     /pseudo
     CDS             complement(8938..9941)
                     /locus_tag="JN692_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(9938..11215)
                     /locus_tag="JN692_001336"
     CDS             complement(9938..11215)
                     /locus_tag="JN692_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263689.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPNTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAICYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYTYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11472..12524
                     /gene="fbaB"
                     /locus_tag="JN692_001337"
     CDS             11472..12524
                     /gene="fbaB"
                     /locus_tag="JN692_001337"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7263690.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            12832..13679
                     /gene="gatY"
                     /locus_tag="JN692_001338"
                     /pseudo
     CDS             12832..13679
                     /gene="gatY"
                     /locus_tag="JN692_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
     gene            13708..14970
                     /gene="gatZ"
                     /locus_tag="JN692_001339"
                     /pseudo
     CDS             13708..14970
                     /gene="gatZ"
                     /locus_tag="JN692_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            14980..15432
                     /gene="gatA"
                     /locus_tag="JN692_001340"
     CDS             14980..15432
                     /gene="gatA"
                     /locus_tag="JN692_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7263691.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15463..15747
                     /gene="gatB"
                     /locus_tag="JN692_001341"
     CDS             15463..15747
                     /gene="gatB"
                     /locus_tag="JN692_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7263692.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            15751..17106
                     /locus_tag="JN692_001342"
     CDS             15751..17106
                     /locus_tag="JN692_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAY7263693.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            17172..18212
                     /gene="gatD"
                     /locus_tag="JN692_001343"
     CDS             17172..18212
                     /gene="gatD"
                     /locus_tag="JN692_001343"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7263694.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18312..19091
                     /locus_tag="JN692_001344"
     CDS             18312..19091
                     /locus_tag="JN692_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7263695.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19173..20072)
                     /gene="yegS"
                     /locus_tag="JN692_001345"
     CDS             complement(19173..20072)
                     /gene="yegS"
                     /locus_tag="JN692_001345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7263696.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMPTGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            20478..20795
                     /locus_tag="JN692_001346"
     CDS             20478..20795
                     /locus_tag="JN692_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263697.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21137..22498)
                     /locus_tag="JN692_001347"
     CDS             complement(21137..22498)
                     /locus_tag="JN692_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7263698.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(22645..22977)
                     /locus_tag="JN692_001348"
     CDS             complement(22645..22977)
                     /locus_tag="JN692_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7263699.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            complement(23168..23890)
                     /gene="baeR"
                     /locus_tag="JN692_001349"
     CDS             complement(23168..23890)
                     /gene="baeR"
                     /locus_tag="JN692_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7263700.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAVSYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(23887..25290)
                     /gene="baeS"
                     /locus_tag="JN692_001350"
     CDS             complement(23887..25290)
                     /gene="baeS"
                     /locus_tag="JN692_001350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7263701.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(25287..26702)
                     /locus_tag="JN692_001351"
     CDS             complement(25287..26702)
                     /locus_tag="JN692_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263702.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(26703..29780)
                     /gene="mdtC"
                     /locus_tag="JN692_001352"
     CDS             complement(26703..29780)
                     /gene="mdtC"
                     /locus_tag="JN692_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7263703.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTEQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(29781..32903)
                     /gene="mdtB"
                     /locus_tag="JN692_001353"
     CDS             complement(29781..32903)
                     /gene="mdtB"
                     /locus_tag="JN692_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7263704.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(32903..34150)
                     /gene="mdtA"
                     /locus_tag="JN692_001354"
     CDS             complement(32903..34150)
                     /gene="mdtA"
                     /locus_tag="JN692_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HAY7263705.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            34429..34485
                     /locus_tag="JN692_001355"
     CDS             34429..34485
                     /locus_tag="JN692_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7263706.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            34757..34813
                     /locus_tag="JN692_001356"
     CDS             34757..34813
                     /locus_tag="JN692_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7263707.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            35085..35144
                     /gene="ibsA"
                     /locus_tag="JN692_001357"
     CDS             35085..35144
                     /gene="ibsA"
                     /locus_tag="JN692_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HAY7263708.1"
                     /translation="MMKHVIILVILLVISFQAY"
BASE COUNT         8765 a         8779 c         9222 g         8385 t
ORIGIN      
        1 gttggccggt ggtggagcag atcttgatgt tagcgtgaaa gaggcggacg gctcggtaac
       61 cacctatctg gtgccttatg cagcggtgcc aaatatgctg caacccggcg tgtcgaaata
      121 tgattttgcg gcgggccgta gccatattga aggggcgagc aagcaaagtg attttgtcca
      181 ggcaggttat cagtatggtt ttaataactt attgacgctg tatggcggca cgatggttgc
      241 taataattac tatgcgttta ccctcggaac gggttggaac acgcgcattg gcgcaatttc
      301 cgtcgatgcc acgaagtcgc atagtaaaca agacaacggc gatgtgtttg acgggcaaag
      361 ttatcaaatt gcctacaaca aatttgtgag ccaaacgtcg acgcgttttg gtctggcggc
      421 ctggcgttat tcgtcgcgtg attaccggac atttaacgat cacgtttggg caaacaataa
      481 agataattat cgccgtgatg aaaacgatat ctatgacatt gccgattatt accagaacga
      541 ttttggccgt aaaaatagct tttctgccaa tatgagccag tcattgccag aaggctgggg
      601 ttctgtgtcc ttaagtacgt tatggcgaga ttactggggg cgtagcggca gcagtaagga
      661 ttatcagttg agttattcca ataactggcg gcggataagc tataccctcg cggcaagcca
      721 ggcgtatggc gagaatcatc atgaagagaa acgttttaat atttttatat caattccctg
      781 tgattggggt gatgacgtta cgacgcctcg tcggcaaata tatatgtcta actcaacgac
      841 gtttgatgat cagggttttg cctcaaataa tacgggatta tcaggaccag tagggagtcg
      901 ggatcagttc aattatggtg tcaacctgag tcatcaacat cagggaaatg aaacgacagc
      961 tggggcgaat ttgacctgga acgcgccggt tgcgacagtg aatggcagtt atagtcagtc
     1021 gagtacttat cgacagactg gagccagtgt ttcagggggc attgtcgcct ggtcgggtgg
     1081 cgttaatctg gcgaaccgtc tttccgaaac gtttgctgtg atgaatgcgc caggaattaa
     1141 agatgcttat gtcaatgggc aaaaatatcg cacaacaaac cgtaatggag tggtggtata
     1201 cgacggaatg acaccttatc gggaaaatca cctgatgctg gatgtgtcgc aaagcgatag
     1261 cgaagcagaa ttacgtggca accggaaaat tgccgcccct tatcgcggcg cggttgtact
     1321 ggttaatttt gataccgatc agcgcaagcc ctggtttata aaagcgttaa gagcggatgg
     1381 tcaaccatta acgtttggtt atgaagtcaa tgatatccat ggtcataata ttggcgttgt
     1441 cggccaggga agccagttat ttattcgcac caatgaaata ccgccatcgg ttaatgtagc
     1501 aattgataag caacaaggac tttcatgcac aatcaccttc ggtaaagaga ttgatgaaag
     1561 tagaaattat atttgccagt aatagtcagg tggttctatg gaaattcgca taatgctatt
     1621 tatattaatg atgatggtta tgcctgtaag ctatgcggca tgttatagtg agttatctgt
     1681 tcagcacaat ttggttgttc aggggaattt tgcacttact caaacacaaa tagcgacata
     1741 tgagcataat tttaatgatt cgtcatgcgt gagtacaaat actatcaccc ctatgagccc
     1801 atcggatatt attgttggac tttataacga taccattaaa ttaaatttac attttgaatg
     1861 gaccaataaa aacaacatca cgttgtcaaa taatcagagc agtttcacca gtggttattc
     1921 ggtaacggtg acacctgcgg ccagtaatgc aaaagtgaat atttctgcgg ggggcggtgg
     1981 ttcagtgatg attaatggtg ttgcgacatt atccagtgct tcatcatcga cacgcgggag
     2041 tgccgcagta caatttctac tgtgtttatt aggtggcaag tcatgggatg catgtgtaaa
     2101 tagctacaga aatgcattgg cacaaaatgc aggtgtctat tccttcaatc tgacattgtc
     2161 atacaacccg ataaccacaa cctgcaaaca ggacgattta ttaattactt tagacagtat
     2221 tcccgtttca caattaccag ccacaggtaa caaagcaaca ataaatagta aaaaaaggga
     2281 tattattctg cgttgtaaaa atttattagg tcaacaaaat caaacatcac ggaaaatgca
     2341 ggtgtattta tcaagttctg acttgttaac caacagcaac acaatactga aaggtgcgga
     2401 agataatgcg taggatttat tcttgaaagt aatggttcgc cagtcacact tttaaatatc
     2461 actaacagca gtaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca
     2521 gatacaacgg tttcaattcc gataacagcc agttactacg tctacgatac aaacaaagtt
     2581 aaatctggcg cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca
     2641 agacgagcaa gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg
     2701 agggggcttt ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc
     2761 gtcggtgtcg ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc
     2821 aggcgcgggc aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat
     2881 ctctgggacg gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc
     2941 atacttagaa attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc
     3001 actggtaacc agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc
     3061 actttgggga tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag
     3121 cgatattttg ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa
     3181 agcatcctta gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg
     3241 aagataacat gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg
     3301 cccatataca caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg
     3361 agagtgttaa atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg
     3421 cctacggtga aaagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc
     3481 agtgtcaggg ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg
     3541 ataaggccac cggttaagcc aaataacaaa atttgccagt tgatgacctc tctaccatca
     3601 aagcgtcgtt taatgtcatt ggcatggtct cgtgcatggg catcctgata ctcgccatgt
     3661 tcgtgatggt gatgatgtcc ttggtcgtgg tgatgttcgt ggtcttgatg atgttcgtga
     3721 tcgtgatgat ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt
     3781 tcgccgcgcc aggtacgcca gaacatccag aacgcggtgc caataatgat cactgcggaa
     3841 atcagctgga gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg
     3901 ccaaaggcaa tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc
     3961 accgcctgtt taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag
     4021 tgccctggtt ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag
     4081 gcgtttcctt gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat
     4141 tcggcaatct gattctactc ccccccagta cctgatacta ctcccccagt agattaatag
     4201 tgctatgatt tttcatattc ttgttaacca ggtgttgcca tgtctcatac aatccgtgat
     4261 aaacagaaac tgaaagcgcg tgccagtaag attcagggcc aggtcgtggc gctcaagaaa
     4321 atgctcgacg agccgcacga atgcgctgca gttttacaac agattgctgc tatccgtgga
     4381 gcggtaaacg gtctgatgcg ggaagtgatt aaaggtcatc tgacggaaca catcgttcac
     4441 cagggggatg agctaaaacg tgaagaagat ctggatgtcg ttctgaaggt gctggattca
     4501 tatatcaaat aatttattaa cgcgattgta aaactgccgt ttttcctcgt ttacaacggg
     4561 tgcgctggac attaccatcc tcctctgcga tttatcatcg caaccaaacg actcggggtg
     4621 cccttctgcg tgaaggctga gaaatacccg tatcacctga tctggataat gccagcgtag
     4681 ggaagtcacg gaccaccagg tcattgcttc ttcacgttat ggcaggagca aactatgcaa
     4741 gtcgacctgc tgagttcagc gcaatctgcg cacgcgttac acctttttca ccaacattcc
     4801 cctcttgtgc actgcatgac caatgatgtg gtgcaaacct ttaccgccaa taccttgctg
     4861 gcgctcggtg catcgccagc gatggttatc gaaaccgaag aggccagtca gtttgcggct
     4921 atcgccagtg ccttgttgat taacgttggc acactgacgc agccacgcgc acaggcgatg
     4981 cgtgctgccg ttgagcaagc aaaaagctct caaacacgct ggacgcttga tccagtagcg
     5041 gttggtgcgc tcgattatcg ccgccatttt tgtcatgaac ttttatcttt taaaacggca
     5101 gcgatacgtg gtaatgcttc ggaaatcatg gcattagctg gcattgctaa tggcggacgg
     5161 ggagtggata ccactgacgc cgcagctaac gcgatacccg ctgcacaaac actggcacgg
     5221 gaaactggcg caatacgtcg tggtcactgg cgaggtggat tatgttaccg atggacatcg
     5281 tatcattggt attcacggtg gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc
     5341 attatcggcg gttgtcgctg cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc
     5401 atctgcctgt cactggatga aacaagccgg agaacgcgca gtcgccagaa gcgaggggcc
     5461 aggcagtttt gttccacatt tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc
     5521 atgaaacgaa ttaacgctct gacgattgcc ggtactgatc cgagtggtgg tgcggggatt
     5581 caggccgatc ttaaaacctt ctcggcactt ggcgcttatg gttgctcagt tattactgca
     5641 ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc gcattgagcc tgattttgtc
     5701 gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg ataccactaa aatcggtatg
     5761 ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa
     5821 aacgtggtac tcgacaccgt tatgctggca aaaagcggcg acccgctgct ttcaccttcg
     5881 gcggttgcta cgctgcgcag tcgattattg ccacaggttt cattaataac gccaaacttg
     5941 cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa
     6001 caagggcgat cgctgttggc gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg
     6061 gatgatgagc aaagcccgga ctggctgttt acccgcgagg gtgaacaacg gtttaccgca
     6121 ccgcgcatta tgaccaaaaa cacccacggc actggttgta cactctctgc ggcgttggct
     6181 gcactacgcc cgcgccatac aaactgggct gacaccgtac aggaggcaaa aagctggctt
     6241 tcatcggcgt tagcccaggc cgacacgctg gaagttggtc acggtattgg tccggttcac
     6301 cacttccacg cctggtggtg agcaccgtac tatagctgct ttggttattg tcagcctttg
     6361 gcactgacat tcagggatga aaccaatgca gctaagaatt accagtcgaa aaaaactcac
     6421 cgctctatta tgcgctcttg ggctaatttc catcgttgca atttatccgc gtcagacggt
     6481 gaattttttc tattcgacag cagttcagat tacagactac atccacttct acggttatcg
     6541 cccggttaaa tctttcgcta ttcgcattcc tgccagttac accattcacg gattagatgt
     6601 ttcgcgctgg caggagcgta tcgactggca gcgggtggca aaaatgcgcg acaacgatat
     6661 ccgcttacag tttgctttta ttaaggctac ggaaggcgaa aagctggtgg acccctattt
     6721 ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg cgcggggcgt atcattattt
     6781 ctccccgtcg gtatccgctt cagttcaggc gcgattattt ctgcaaacgg tggatttctc
     6841 acaaggcgat ttccctgccg tgctggacgt agaagaacgg ggaaaattat cggcaaaaga
     6901 attacgcaag cgggtaagtc agtggctaaa aatggtcgaa aaacgcacgg gaaaaaagcc
     6961 gattatttac tcaggagccg ttttttatca caccaatctg gcgggctatt tcaatgaata
     7021 tccgtggtgg gtggctcact attatcaacg tcgtccggac aatgacggca tggcctggcg
     7081 cttctggcag cattccgacc gtggacaggt agatggcatc aatggtccgg tggattttaa
     7141 tgtgtttaat ggcacggtgg atgagttgca ggcattcgtt gatgggatta aagaaacgcc
     7201 ttaaataaga agcagtgata tactcccacc agactccctg gcaggaaaaa gacaaatgga
     7261 acaagcgcat acccagctga ttgcccaact gaacgagcgt attttagcgg cggataacac
     7321 gccgctttat attaagtttg ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga
     7381 gcatggcaat attttgcccg gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat
     7441 tacggtgcgc aaggcgatgc aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg
     7501 ttacggcacg cagatcaaca acatcttcga atactcgttg aaagaagcgc gaggtttttc
     7561 tcagcaggtg gtattgcgcg ggaaaaagcc cgatacgcta tgggttaata agcgtgtcgt
     7621 gaaatgtccc gaagaagtcg cgcagcagct ggcggtcgaa gcgggaagtg atgtcttttt
     7681 gcttaagcgt attcgctatg tcgatgaaga agcggtatcg attgaggaat cgtgggttcc
     7741 ggcgcattta attcatgatg ttgatgccat cggaatttct ctttatgact atttccgcag
     7801 ccagcacatt tacccacaac gtacgcgttc ccgcgttagc gcccggatgc cggatgccga
     7861 gtttcagtca catattcagt tagatagcaa aataccggtg ctggtgatca agcaagtggc
     7921 gcttgaccaa cagcaacggc ctattgagta cagcattagc cactgtcgca gcgatctata
     7981 cgtttttgtg tgcgaggagt agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc
     8041 cgacaaccca cgacgccact gcattgccca gtaatacggc atccgccagt ggcagaccag
     8101 aggccagccc ggcaagtacg ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa
     8161 cttgcgttgg aaatgccgga atgcagccag aagcgtcgtt gctgaaatac catgcgcctt
     8221 ctttatcgag acgaacgatc aacggcgcgg caaatttctc ctgccattgc ttgccaagtg
     8281 ttgttatctc tgcggataaa gcaaaacgtt cggcggcaat ctcagcctct tgacgattga
     8341 gtgacactaa aggtcgacag gccatgatcc gcgccagtaa tgcgtccggg atatcgccaa
     8401 tacgtgggcc aaaatcgata aacggcgtca cgtcttgcag cttttccagc cattccacta
     8461 acaattcgcc gcagggcgag gccagttgat aaccggaaaa atagagcagg ttgccaggcg
     8521 caacggttaa tcgtgccaac cattggcgat tccattgatt ttccacaccg ctgaatgaca
     8581 taaaagtgcg ttcgccatcc ggctcaacca gtgccagaca ccagccgtta tcgctttcgg
     8641 cgttatcgat caggctgatt aacccctctt tcgccatccg gttgcgaata atctccgccc
     8701 acacgccctg accgagcggc aaggcattac ccgcttcgat gccgaggcgc tttaacgcca
     8761 cggcaatatt cagtgcgcag ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc
     8821 acccacgcca ggggagggca taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga
     8881 ccatcaccgg ctgacgcgtg gttaattcag gcagcagtgt gtgtaatcga gcgccgctca
     8941 taccgcctcc cgttgttgac gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag
     9001 ctgatttacc gcatccagct ccgcctttaa cgcaggatcg acagcgttaa tgccatgcaa
     9061 cgcgccgcaa atcgccgtcg ccatagcacc tatggtgtct gtgtcgccgc caagattggc
     9121 gcacaggacg gcgcagcggt ttgggtcggt ttgtgccagt tcaactagcg caatggcgca
     9181 cggaacggac tcaatagtgc tggtgcctgc gccaacgact tggtaaagct gttcgctggc
     9241 ggattcggtg ccgtcggcat tacgcacaat tttcagcgca atctccagac gtgccgccag
     9301 tgaggcgctg aatgtggtgg tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaatga
     9361 atcaacaatc gctgaccagc gttctccgtc aatggcacga gaaatcgccc atgcgatgac
     9421 taccgcgcct gcaaccgcca gatcggattt atgggtcggg ctggacgcca gcgccacatc
     9481 atcaataaag gaatcaacat cacgcgctgg aagcaaacaa cctaatggcg agacgcgcat
     9541 cgctgcgccg ttggttacgc cgttgttttc cagttcagca acgggtttac cgtcgcgaat
     9601 ggcgttaagc gcaatcttcg aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg
     9661 caacgcccag tcgagaatat tacgcccaat cagattcgga tcgatcttgc cttcacattc
     9721 cagtaacgca tccgccagac acaggccatc gaggtatcgt cggtgaattc ggcgcggtta
     9781 aaataacagg ccgcgttatt ctcctttggt ccaggcagaa aacggtcaat ccagccaaag
     9841 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
     9901 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca
     9961 acgcaatatc tcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa
    10021 aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg
    10081 accagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca
    10141 caccgccaag acgatagccg agcaaactgc cgaagccttg gcagcagagc gtgatcagcc
    10201 cctgcgcagc ggtacgcata tgcacggggg cttttttatc gacataggtg taagcggtaa
    10261 cgtagtaaaa atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg
    10321 tgaaatattc atccgcacta ccgtaaataa agaagccata gcagatcgca gcggtgacca
    10381 gaccaagcaa taataccttt ttgataccaa agcgtttagt gaaaaacggc aatgccagca
    10441 taaagaagat ttcagagaac tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa
    10501 cttcggtcag ataaccgttg gcaaagatgt aatagaacgc cagtggcatc gcaaacagga
    10561 atgaacagaa gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc
    10621 cgagcatgac tttaatgtcc attttgccgg tgctttttgg cggcgtgttg ggcaggaaaa
    10681 acgcaaacac accgagcaga gcagaacttc cggcggtaat tagcagcggg atgttagtcg
    10741 gtgagatatc ggcatacccc agcatttgcg gcaagaaacc acatgccaga ccggaggcaa
    10801 tccagccgat agtgcccatc acacgaatgc gtgggaaatc acgttccaca tccggcacgt
    10861 tggcaaaagc gatgctgtta gtcagcgcaa tggtcggcat ataggttagc gagtaggcca
    10921 gcagtaacgg aaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcacag
    10981 caccagcgaa catcaatacc gccagcactt tctgcgccga gaaaaagcgg tcagtgatgg
    11041 agccaaccag aattggcgac aggatcgccg caatggctgt gcaggcatac gaccagccaa
    11101 tttctccggc actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc
    11161 cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt
    11221 aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacctaa taatgccttt
    11281 tgaatacctt tgcggtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa
    11341 cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta
    11401 tgattgcccg caaaaggcgg gccaggacaa tccatagccg atatccaatc ggaatttacg
    11461 ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac
    11521 agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat gactacgtag
    11581 accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg cagacgttgt
    11641 ataacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg
    11701 ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca
    11761 ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac ggcgtgttgg
    11821 tgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt aatcacaacg
    11881 agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggcct
    11941 tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc
    12001 gccagattga agaaatttct gcggcctttg aacgcgcgca cgagctgggc atggtgacag
    12061 tgctgtgggc ctatttgcga aactccgcct ttaagaaaga tggcgttgat taccatgttt
    12121 ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca gatatcgtca
    12181 aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt tataccgacg
    12241 atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag
    12301 ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca ggcggtgaaa
    12361 ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc ggaatggggc
    12421 tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaatg
    12481 ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc tcatacgaac
    12541 agcaccaccc cgtaggacgg ataaggcgtt tacactgcat ccggcaacct atgcctgatg
    12601 cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc
    12661 ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt
    12721 tcactcctcg aacaattttc atattgtcgt ttttgtgatc gttatctcga catttaaaag
    12781 caaataattt cattatattt tgaaatcgaa agcaaacgac aagatatgaa aatgtacgtg
    12841 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
    12901 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
    12961 ccggtcatca tcgccggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg
    13021 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
    13081 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
    13141 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    13201 tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg ccaggaagat
    13261 gatgtgcaag tcaatgaagc cgatgcgttg tacaccaacc ccgcacaggc gcgtgaattt
    13321 gccgagggat tgattccctg gcggtcgcca tcggcacggc tcacggtatg tatgccagcg
    13381 caccggtgct tgatttttct cgactggaga acattcgcca gtgggtgaac ttaccgctgg
    13441 tgctgcatgg cgcgtcagga ttatcgacta aggatattca gcaaaccatc aaactgggga
    13501 tatgcaaaat caacgttgca acggagctga aaaatgcctt ctcgcagtcg ttaaaaaatt
    13561 acctgaccga gcaccccgaa gcgaccgatc cccgggatta tttgcagtcg gctaaatccg
    13621 caatgcgcga tgtggtgagc aaagtgattg ccgattgtgg ctgcgagggc agggcataac
    13681 gcacctgcca tttaacaagg aaaaaacatg aaaacgttaa ttgcccggca taaagctggt
    13741 gaacatatcg gcatatgttc agtctgttct gcccatccgt tggttatcga agcggcgctg
    13801 gcatttgatc gcaacagcac gcgcaaagtg ctgattgaag caacgtcaaa ctaggtcaat
    13861 caatttggcg gttataccgg aatgacaccg gcagactttc gcgaatttgt ttttacgatt
    13921 gccgataaag ttgggtttgc acgcgaacgc attattctcg gcggcgatca tctggggcca
    13981 aactgctggc agcaagaaaa tgcggatgcg gcgatggaaa aatccgtcga gctggtaaaa
    14041 gcatatgttc gtgctggctt cagtaaaatt catcttgatg cgtcaatgtc ctgcgcgggg
    14101 gatcccatac cgttagcacc agaaacggtt gcggaacgag ctgctgtgct ttgctttgct
    14161 gcggaaagtg tggcgacaga ttgccagcgt gagcaactga gctatgtcat tggcactgaa
    14221 gttccggttc cgggcggtga ggccagcgcc attcagtcag tacacatcac ccatgttgaa
    14281 gatgccgcca atactttacg tacgcatcaa aaggccttta ttgcccgtgg gctgacagag
    14341 gcgttaacac gtgtgattgc catcgtggtg cagccgggtg tggaatttga tcacagcaat
    14401 attatccatt atcagccgca ggaagcgcag gcgctggcgc aatggataga aaacacccga
    14461 atggtttatg aagcacattc taccgattac cagacccgga cggcttattg ggaattagtc
    14521 cgcgatcact ttgcaatatt gaaagtcggt cccgcattaa cctttgcttt acgcgaggcg
    14581 atatttgcac tggcacaaat tgagcaggaa cttatcgccc ccgaaaatcg cagcggttgc
    14641 ctggcggtaa ttgaagaagt gatgctcgac gaaccgcaat actggaaaaa atattatcgc
    14701 acgggtttta acgattcatt actggatatt cgttacagcc tgtcggatcg tattcgttat
    14761 tactggccgc atagtcggat taaaaatagc gttgaaacga tgatggtgaa tctgcaaggc
    14821 gtggacatcc cactgggcat gattagtcag tatcttccca aacaatttga acgcattcag
    14881 tccggggaat tatcagcaat gccgcatcag cttattatgg ataaaattta tgatgttttg
    14941 cgcgcctatc gctacggctg tgcggaataa ggacggtata tgactaacct gtttgttcgt
    15001 agcggaattt cttttgtcga tcgtagcgaa gttttaacct atatcggtaa tgagatgctc
    15061 gcgaaaggtg tggttcatga tacctggcca caggcattaa ttgccagaga agcagaattc
    15121 cctaccggga taatgcttga gcagcacgcc attgcaatac cgcattgtga ggcgattcat
    15181 gctaagtcgt cagccattta tctgttaagg ccaacaaata aagttcattt tcagcaagcg
    15241 gatgatgata acgacgtggc ggtatcgttg gttattgcgt tgattgtgga aaatccgcag
    15301 cagcaattga aacttttacg ctgtttattt ggcaagttac aacagcccga tatcgtcgag
    15361 acactaatta ctcttcctga aactcagtta aaggaatact tcacaaagta tgttttagat
    15421 tcagacgaat aaatccctct gtaacaataa aaaggactat ttatgaaacg caagattatt
    15481 gtcgcttgcg gaggcgcggt tgcgacctct acgatggcgg cggaagaaat taaagagttg
    15541 tgtcagagtc ataatattcc tgttgaatta atccagtgtc gggttaatga aatagaaacc
    15601 tatatggatg gtgtgcattt gatatgtacc actgccaggg tggatcgtag ttttggcgat
    15661 attccgttag ttcacggcat gccttttgtt tctggtgtcg gtatcgaagc attacaaaat
    15721 aaaattctga ctatcttaca ggggtgacct atgttttcag aagtcatgcg ttatattctc
    15781 gacctcggcc ctacagtgat gctgccggtt gtcatcatta ttttttctaa aatattaggc
    15841 atgaaggcag gcgattgctt taaagcgggt ctgcatatcg ggattggctt tgttggcatt
    15901 ggccttgtga ttggcttaat gctggattcc attggtccgg cggcgaaagc gatggcggaa
    15961 aatttcgacc tgaatctgca tgtggtcgat gtcggctggc cgggctcttc accaatgacc
    16021 tgggcgtcgc aaattgcgct ggtggcgatt ccgattgcga tattggttaa cgtggcgatg
    16081 ttactgaccc gtatgacgcg ggtggtaaat gttgatatct ggaatatctg gcatatgacc
    16141 ttcaccggcg cgttgctgca cctggcaacc ggttcatgga tgatagggat ggcgggtgtg
    16201 gtaattcacg cggcgtttgt ttataagcta ggcgactggt ttgcccgcga tacccgaaat
    16261 ttctttgagc tggaaggtat tgctattccg cacggtacgt cggcgtatat ggggccgatt
    16321 gcggtgctgg tcgatgctat catcgagaaa atcccaggcg ttaaccgaat taaatttagc
    16381 gccgacgata ttcagcgcaa atttggccct tttggcgaac ctgtcaccgt gggttttgtg
    16441 atggggctga ttatcggcat cctcgcgggt tacgatgtca aaggtgtatt gcagctggcg
    16501 gtaaaaacgg cggcagttat gctgctgatg ccacggatga ttaaacccat catggatggt
    16561 ttaacgccca tcgctaagca ggcccgtagt cgtttacagg cgaagttcgg cggtcaggag
    16621 ttcctgattg gcctggatcc cgcattactg ctggggcata cggcggtggt atcggcaagc
    16681 ctgattttta ttccgctcac cattttaatt gctgtttgtg ttccgggtaa tcaggtactg
    16741 ccgtttggcg atcttgccac aatcggcttc ttcgtggcga tggcggtcgc ggtgcatcgt
    16801 ggaaatctgt tccgcacctt aatctcgggt gtcatcatta tgagcatcac cctgtggatc
    16861 gcgacgcaaa ctattggttt gcacacccaa ctggcggcta atgctggggc gttaaaagcc
    16921 gggggtatgg tggcttcaat ggatcagggc ggttctccca ttacctggtt actgattcag
    16981 gttttctccc cgcaaaatat tcccggtttc attattatcg gcgcaattta tctgaccggt
    17041 attttcatga cctggcgtag agcgcgtggt tttactaaac aagagaaagc cgttctcgca
    17101 gaataatttt tacctgaggg ggaggttatc ccccttaaca atcaggagtt tttatgaaat
    17161 caggagtttt tatgaaatca gtagtgaatg atactgatgg tatcgtgcgc gttgcaaaaa
    17221 gcgtcattcc tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc agctcgggct
    17281 tatgtggttc cgatttaccc aggatattta aaaatggtgc acattattat ccaataacgt
    17341 taggccatga atttagcggc tatattgatg cggtgggatc cggtgttgat gatttacacc
    17401 ctggcgatgc ggttgcctgt gtgccgttat taccctgttt tacttgtcca gagtgtttga
    17461 aagggtttta ttcccagtgc gcaaaatatg attttattgg ctcgcggcgt gatggtggat
    17521 ttgctgaata tattgtcgtt aagcgaaaaa atgtctttgc tctacccacg gatatgccta
    17581 ttgaggatgg ggcttttatt gagccgatta ccgttggcct gcatgctttt catttagcgc
    17641 aaggttgtga gaataaaaac gttattatta ttggtgccgg aaccattggc ctgctggcta
    17701 ttcagtgcgc tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt agctcagaaa
    17761 aactggcact ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc gaaatgagcg
    17821 cgccgcaaat gcagagcgtt ttacgcgaac tgcgctttaa tcagcttatc ctcgagacgg
    17881 ctggcgtacc gcaaactgtc gaactggcgg tagagattgc cggtcctcat gcccaactgg
    17941 cgctggtggg cacgttgcat caggatctgc atttaacatc ggcaacgttt ggcaaaatat
    18001 tgcgtaaaga gctgacggtt atcggcagtt ggatgaacta ttccagccct tggccggggc
    18061 aggagtggga aacggcgagc cggttgctga cagaacgtaa gttaagcctg gatccattaa
    18121 tcgctcaccg tggaagcttt gaaagcttca cccaggcggt gcgtgacatc gctcgtaatg
    18181 ccatgccggg caaagtgttg ctcattccat gaaaccgcgg gccagcgtga tgctggcccg
    18241 gtattgtgca aaacagatca ttcaccaatg gtcccccttc gtttacacta gccacaattg
    18301 aatatgggta aatgacgatg aattcattcg agcgaaggaa taagatcatc caattagtga
    18361 atgaacaggg aaccgtgctt gttcaggatc tggcgggagt atttgctgcc tcggaagcga
    18421 caatccgtgc cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc tttcatggcg
    18481 gtgcggcgaa aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc gggtttaaag
    18541 agcgatttca gctcgccagc gcgccaaaaa acagaatagc gcaggcggca gtcaaaatga
    18601 tccatgaagg gatgaccgtt attctcgaca gcggaagtac cacaatgctt atcgccgaag
    18661 gattaatgac cgctaaaaat atcacggtga ttaccaacag tctcccggca gcgtttgccc
    18721 tttccgaaaa taaagacatt actctggtcg tctgtggtgg cacggtccgc cataaaacgc
    18781 gctcgatgca tggttccatt gccgagcgtt cattgcagga tattaatgcc gatttaatgt
    18841 ttgtcggtgc tgatggtatt gatgcggtta atggtattac gacctttaat gaaggttatt
    18901 cgattagcgg ggcgatggtc accgccgcaa ataaagtgat tgccgttctc gattcatcga
    18961 aatttaatcg tcgcggtttt aatcaggtac tgccaatcga aaaaattgac attattgtta
    19021 ccgatgatgc tgtgtcagag gtggataaat tggcattgca gaagacacgg gtaaaattaa
    19081 ttacggtgta gtattgttct gtatgccgga taaggtgttt acaccgcatc cggcattacg
    19141 gattctccct ggttcaagag agaatagttt gattaacgca ataatggaca atctggtggt
    19201 aatcgacaac gcaacgccgc cggaagtatt tcaatatgga agttttgccc actcaacggt
    19261 tcgccatcaa gattaaaagt gatttcgtgt ggggcttgta tatcaaacca cgacgaagcg
    19321 ccttcgataa tattcgggtt atcttcgtca gattttaagg ttgatacgag agccggaaga
    19381 atttcatcgc cggtaaaaat gcgcagttgc agcaagccat cgttaattaa cgcgttcggg
    19441 cacaattgtt gaccgccacc ggcctgacgc ccgttaccaa taccaatgac cagggcgtca
    19501 ccttgccagt gaaagttttc accgcggatt tcacaacggt ccggttgcag agtatccatg
    19561 cgcattaagc catgaatgat gtaagagacg ccacccagcg cggcttttaa tttttccggc
    19621 gtttctgtgg taatacgcgt cccaaatccg cctgtcggca tattaataaa acaggtttgt
    19681 ttgttgacct gcgccatatc tatcgcaatg gcattaccgg caattgccag tttcagcgcc
    19741 ttatccagtg cctcaggaat ccctacactg gtggcaaaat cattggcggt tcctaatggc
    19801 aaaattccca gcgcgggtat gtcatccccc tcacactgaa tcaacgccgt agaaacttca
    19861 ttaatggtgc catcgccacc accggcaatc accgttgcga cgcccaactt ccgggcctcc
    19921 tctacaaatc gtgcggcatc acctttctcc caggtgaccc gcacatggat cgtcattcct
    19981 tcctcacgca acagcataat tgcttcgcgc aagggtagat tgtcagtact tttgccatta
    20041 agaatcagta agctggcggg aaattctgcc atgatcgtat gtgcctttat gattggtctg
    20101 tagatagtgt agagcagaaa acaaaaggtg gagtcagaac aggatgaaag tcggaggata
    20161 aaaagtcagt tcaagtatgg aggattcagt ggttaccacc aggagcttgc aatggggaca
    20221 gcgaatacta acggctgcca ccgataaatt tcaaaaaaga gcatatacct aatattcaac
    20281 taaacagtgg catcttcaat ataatatatt aaagccccca tggagttacc ctgaagggcc
    20341 tcaatgtccg taattcctac ttatgtagga aatgttgtac agaacattta ttataatcct
    20401 attcaaatat aataatcatg ccattattat atttaaacac tagagagtgt cgttggtatt
    20461 taatggggga aggtgagatg aaaaagatag ctgctatatc attaattagt atttttctta
    20521 tatctggttg tgctgtgcat aatgatgaaa caagtatcgg taaatttggt cttgcatata
    20581 aaagtaatat tcagcgtaaa ctcgataacc aatactacac cgaagccgaa gcttctttag
    20641 ccaggggcag aatatctggt gcagaaaata tagtaaaaaa tgatgcagcc catttctgtg
    20701 ttactcaggg caaaaaaatg cagatagttg acctgaagac agaaggtgca ggattacatg
    20761 gcgtcgctcg tctgacattc aaatgtggag agtgagaata ttttttggta agcgtcaaac
    20821 atgcgcgttc tggctgtgcg tagccggaac ctgggcgagc acgataccgt tacgtgaaag
    20881 gcattgtgct atgaaggggg attctatcga tgtggtcaat ggaagacggt tatcagggat
    20941 caatccttct ttacagctta tgtacaaaaa ataagcccgt gtaagggaga ttacacaggc
    21001 taaggaggtg gttcctggta cagctagcat tttatgggtt atgtttttca gcgaaacgga
    21061 tgataacctt aataaatgca gctgtatgtg atcggtttct aagaattttc catccgggaa
    21121 aaataatcga aattaatcac ttaccgtggg ggttacgcgt ggtttccccg gagaaattac
    21181 gcattagcag cgcgtaattt agctcaagat cctgcgggac cgggagccac acagtataac
    21241 catcgcctgg tgcgaccggc atagcttcgc ctttggcgtt ttccatgtgc tcaagggtaa
    21301 agttaatgtt gccttgcggc gtcatcagct caagactgtc gccaacggag aatttatttt
    21361 tcaccgctac cgctgcgagg tcccccttgc gctcaccggt aaactcacca acaaactgct
    21421 ggcggtcaga aactgaataa ccgtattcgt agttctgata atcgtcgtga gtatgacgac
    21481 gcaggaaacc ttcggtatag ccacgatgcg ccagaccttc cagagtttcc agcaggctgg
    21541 tatcgaacgg ttttcccgca gcggcgtcat cgatagcttt gcggtaaacc tgtgcggtgc
    21601 gtgcgcaata gtagaaagat ttggtacgac cttcgatttt cagcgaatgc acgcccattt
    21661 tggtcaggcg ttctacatgg gcgatggcgc gcagatcttt cgagttcatg atgtaagtgc
    21721 cgtgctcatc ttcaaacgcg gtcatatact cgcccggacg ctgggcttct tcgatcataa
    21781 acactttgtc ggttggcgcg ccgataccca gcgtcggctc aacattttgc accggaatcg
    21841 gctcgtactt gtgtacgatg ttgccaacgt catcttcttt cccttcctgg acgttgtact
    21901 cccagcggca ggcgttggtg caggtgccct ggttcgggtc gcgcttgttg atatagccag
    21961 agagcaggca gcgaccggag taggccatgc acagtgcgcc gtgaacgaag atctcgatct
    22021 ccatatccgg cacctgatta tggatctctt caatctcttc cagcgacagc tcgcgagaga
    22081 ggatcacgcg ggtcagtccc atttgctgcc agaatttcac cgtcgcccag ttcacggcgt
    22141 tagcctgcac cgagaggtgg atcggcattt cagggaagtg ctcacgcacc agcataatca
    22201 gccctggatc ggacataatc agcgcatccg gccccatttc caccaccggt ttcaggtcac
    22261 ggataaaggt tttcagcttg gcgttgtgtg gtgcaatgtt gaccacgaca taaaactttt
    22321 tccccagcgc gtgggcttca ttgatgccga gctgaagatt ttcgtggttg aattcgttgt
    22381 tgcgcacacg tagggagtaa cgcggctggc ccgcataaac agcatctgcg ccataagcga
    22441 aagcgtaacg catatttttc agcgttcccg ccggggaaag gagttccggt ttaaacataa
    22501 ttctctcgtt ctgatgacag gtcagatccg cttcacctga tgaagcggtt aggggaggcc
    22561 cccactttaa gggcgggcat tgtagcgctg taggggggat ctggtaaagc gccggacgcc
    22621 aacaacccac gcccggcatc gtaattacgt attgtctttc accgtctggc ttgtgccatt
    22681 ggctttaacg gaggcaattc cggtttcacg agattgcgcg gtggcgtaca tctggctgga
    22741 gccgataatt tgatgattag cggctttcag attgaaatag aatttgccgt tgcttgccgt
    22801 ttttttctca tagcgctctt cttgcgggct gttgctacgc accgacgcga tgcccttttc
    22861 cgcagaggct tttgaggtat acagctcgct ggtgaggata gtctcaccat tgcccgcttt
    22921 tagcacaaac cggaactgat tatcactgct cttactgagt tcaaaccaac cagccataat
    22981 gactccttga ttgaaattgc ttatcgaaaa cagcatctaa aggtgctgtt tactgattaa
    23041 agtatgagcg attttctgat gcctgccagg gcggagagat aatgtcagtt tgctgaccat
    23101 gcgtggaata atgttgccgg atcgggtctg aacgccatct ccggctaaca aagtaatgtc
    23161 gctaaaacta aacgatgcgg caggcgtcgg cttcccagcg gtaaccaacg ccgtaaacgg
    23221 cgcggataaa tgactgttcg gcgtcgagag attccagctt gcggcgcaga tttttaatgt
    23281 ggctgtcaat ggtgcggtcg gttactacgc ggtagtcgtc ataaagatga ttgagcaatt
    23341 gctcgcggga gaacactttt cctggttcgt gagagagcgt tttaagcaga cgaaattccg
    23401 caggcgtcag gtcaagcatt ttaccgcgcc atgaagcctg aaaacgacct tcgtcgataa
    23461 tcaacgggct ttcggcatcc tgttgctgaa gttcgcgttg cggtttacaa cggcgcaaaa
    23521 tggttttgac acgcgctacc acttcccgtg ggctgtacgg cttgcagatg tagtcatcgg
    23581 cgccaatctc cagccccagc aggcggtcga tctcttcgat ttttgccgtc accatcacga
    23641 tcggaacatc ggaaaaacga cgaatttccc ggcataatgt caggccatcg gtgccaggga
    23701 gcatcagatc caacaggatc agatccggtg gtgtctggcg cacatacgcc agtacctgat
    23761 cgccgtggct gataagcgtc ggcgcatagc tcacagcacg cagataatca atgagcaact
    23821 gccccagctt cggttcatct tccacgatca aaatacgcgg tgtgttttcg tcgattggta
    23881 actcggtcat acttctctct gtaaatcccg ttccagcggt aactctactg taatgcttac
    23941 cccgccaaaa ggcgaatggg cggcaataat gcggccatta tgggcttcaa caatgttcag
    24001 gcaaatcgcc agccccagcc cggaaccgcc gctggcgcgg ttgcgggaac cttcggtgcg
    24061 ataaaaacgt tcaaacaatt tttgtagctg atcgtcactg acacctggcg cactgtcggc
    24121 aaaggtcagg cgcaccgttt tgtcgtgctg ctcggcagag attttcaggc tgccgccgct
    24181 gtcagtgtag cgcaggctgt tttccagtaa gttattgaat aactgcatta aacggtcgcg
    24241 atcgccaaat acggtgatac tgtctggcag ggaaaattgc agtttcaggc cacggctggc
    24301 gaatcgttcg cgaaatgcgc cgcccgccac ttccagcagt gggatcaaat ctaccggtgc
    24361 tttttgatag gcgagagcgc cttcatcaga catcgacaac tgatgaagat cgtcaaccag
    24421 tttggtcagt gtaccgacct ccgcctgtaa agacgccacc gtctccggcg tgaatttgcg
    24481 cacgccatcc tgaatggctt ccagttcacc gcgcagcacc gctaatggcg tacgcagttc
    24541 gtgagaaata tcggccataa aatcgcgccg catttgctgg tttttctcca gcgtgctggc
    24601 gagctggttg aagtcttgcg ccagtttgcc cagttcatct tcactggtgg gcgttacgcg
    24661 ggtagtgaaa tcgcccgccg ccagtttgtg cgtgccatcg acaagtcgtt ttaccggtgc
    24721 cagcaaaccg cgcgccagca gaaaggtggc gagcgccgcg agtaacgttg ccagggcgac
    24781 gatcagccag ctggtttgcc gctgttgttt atcgaaattg atatcagtat tgcgggtcag
    24841 ccgctcaaca ggggaggcaa tcaccgcgcc cacttccgca ccgttgacca gaatgggtcg
    24901 ccgtgtaccg tcaggtggaa tcggcgctcg cggaccaacc agtactttgt tgttttgatc
    24961 gaccacccag aactgggtac gccagccgtg cggcggcata cccgggccgg gtttatcttc
    25021 cgaattatcg tgttcaaatg aacgcaggat ctgaaagaca aagcgatcgt tgttgcgcag
    25081 gaagcgccag ttaccatgct gcgcatactg ctcgccaagc gcatcactta acagctgtaa
    25141 tcgctgttca ttgccgtgtt tgatgtaatc aataaagcca cgctcaaaac tgatacgcac
    25201 cgcccagtgc atactgatca gcaagacaat gcaggtggcg aaaatcgcca gaaacagttt
    25261 gccggtaata ccgggtcgcc agaacttcat tgcgcgctcc tttttcgccg cgaaatagct
    25321 acattttgat gcgtatcgtt cggcactctg gcaaagatga acgccggaag ggcgatgata
    25381 aacgccatgc taagccaggt gtacataaag acggtttgtg tggtgccgct gtcgacgctg
    25441 acatgctgcg aaccaaaaag ccccagcaac agcccggcga tggtgacgcc gatactcatc
    25501 gacaattgca taatcatcga cagcaggctg tttccgctgc tcgccagatt gtccgggaga
    25561 tctttcagcg tcagggtgtt catggaggag aaacgggtcg agttgaccat cccttgtaaa
    25621 aacaggacga acggcaaaac gtagtaccag cccaacagag cggtagtcat aaacaacagg
    25681 gtgaccagcg acagacccag cgtggtcgct accagtaccc gacgataacc aaagcgattc
    25741 accacctgta ccacaattcg cttcattccc atgctgccaa gcaccatcgg gatcatcatc
    25801 agtccggcat gaaacggcga gaaaccgagg ccaatttgca ggaaaaccgg tgtcataaag
    25861 ggcaacatgc cactgccaat acgtccggca aagctccccg ccaggcccag cgaaaaggta
    25921 cgagtgcgga acagtttcag actgaacagg gcgcggttgt tatttctggc gtgcagcaga
    25981 taaagcacca gtgccaccac gccaactgcg accaggccag caatcgccag cggcgataaa
    26041 cctgtacctt tactgccgtc cagcgccagg gttaataccg ccatgccaac cgccagcaat
    26101 aaaaagccgg aaagatcaaa gcgccgcgtc tgcatggtgt agttcggcat taacatcaat
    26161 gtggcgatcg caccgataat ccccaccgga atgttgatca aaaagatcca gtgccacgac
    26221 gcgtattcta ccagcagacc gccgagcgcc ggaccgagca gcggaccgac ctgaccgggt
    26281 aacgtgacaa aggtcatcgc cgccatatat tgctcgcgcg gtacgatttt catcaccgtc
    26341 aacctgccga ccggcaccat catcgcaccg ccaacgccct gtaacgcgcg tgccagcaac
    26401 agttcgttca gcgtgccgga aagcgcgcaa aacagtgaac cgagagtaaa cagcacgatg
    26461 gcggtaaaga aaatattgcg cacgccgact ttgtccgcca gccagccgct ggcgggcagc
    26521 atcaccgcca cggtcagcac ataagagaca atgaccatgt gcatatgcaa cggactttcc
    26581 ccgaggcttt gcgccattga gggaagggcg gtgtttacga tggtggtgtc cagcgactgc
    26641 ataaagaagc cgaaagccac aatccataat tgccagcggg tgctgtcggg aagatctgtc
    26701 atttactcgg ttaccgtttg tttaggttta cgcgaaaaac gcagccgcag acggtcgaaa
    26761 aagagataca ccaccggcgt ggtatacagc gtcaggagct ggctcatcac cagaccgccg
    26821 acaatggtga tccccagggg ttgccgcagc tccgagccgt caccgcccga caataccagc
    26881 ggcagcgcgc caaacagcgc cgccagggtg gtcatcataa tcgggcgaaa acgcagcaga
    26941 caggcctgga aaatagcttc ctgcggcgtc aggttaccgt gccgttgggc ttcaagcgca
    27001 aaatcgacca tcataatggc gtttttcttc acgatgccga ttaatagcat gatccctatt
    27061 agggcgatta ggctgaacgg ggcattgaac agctccagcg ccaacagcgc tccaacgccc
    27121 gccgagggca gggtggagag aatcgtcagc ggatgtacgt aactctcgta aaggataccc
    27181 agcacgatat acaccgtggc gatggcggcg ataatcagga tcacctgcga gttcatcgtc
    27241 tcctggaaaa cctgctccgt gccggcaaaa ctgccgcgca ccgtcgaagg cacaccaagc
    27301 tgggtcattg cgcgatcgat cgccgcactg gcgtccgaga gcgattttcc ggtcggcagg
    27361 ttgaacgaga tggtcgaggc cgccgataat ccctgatgat tcaccgatag tggggcattc
    27421 gccggttgcc atttagcgaa atacgacagc gggatcgctt tgccttcgtt attgataacg
    27481 aacatttttt ccagcgcact gatgtcctgg gtatagcgcg gatccacttc catcaccact
    27541 ttatactggt tcatcggctg gtaaatggtc gagatttgcc gctgaccgaa ggcgttattt
    27601 aacagactgt tggcggcctg tacgtcgatt cccagccgtg ccatggtgtc gcggtcgtaa
    27661 accagattca tctccgcgcc gttatcctgc tgatcggagt tcacgtccgc cagttccggc
    27721 aacgtcgcca gttttttgcg gattttcggc tcccattcgc gcagtgccgc caggtcgtcg
    27781 gataacaacg tgtactggta gctggcgttc gactgacgcc caccaacgcg aatatcttgt
    27841 accgccatca ggaacagatt cgcccccggt tctttcgcca gcttcacgcg cagacggtcg
    27901 ataatttgct gcgccgtttc gctgcgttcg tcgcgtggct tgagggtgat aaacatcatc
    27961 ccactgttaa ctcgtgaacc gccggtaaag ccggtgacat tatccactgc cggatcgtca
    28021 cggataattt tcatgaaatc ctgcaatttg ccgcgcatcg cctgaaacga aatactctga
    28081 tccgcctgta tcccacccat cagtacgccg gtgtcctgct ccgggaagaa ggttttcggg
    28141 atcgagatat acagccagat attcagcgca atggtgccaa gcagcaccac gcccaccaga
    28201 cgggtatgat tgagcaccca tttcagcgac ttgccgtagc cttgttgcag ggctaccaac
    28261 atgcggccaa aaccacgcag tcgcttttgc tcacgcggct tgctggcttt tagcatccag
    28321 ccgcacatca ttggcgttag cgtcagggaa accagcaacg aaataccaat ggcgacagaa
    28381 agcgtcacgg caaactcgcg taacagtcgg cccggcaatc cgcccatcaa cagcagcggc
    28441 aggaacaccg ccaccagtga cagactcatc gacagcaccg taaagccgac ttcgcgagta
    28501 ccttgcagcg cggcctgtag tggtttcatc cccgcttcca gatgacgggc aatgttttcc
    28561 agcaccacaa tggcgtcatc caccacgaaa ccagtagcaa tggtgagcgc catcagcgaa
    28621 agattattga gactgaatcc gcacaggtac atcgccgcaa acgtaccaat cagcgaaacc
    28681 ggcaccgcaa cggcgggaat aatagtggcg cgacccgagc gcaggaataa aaagaccacc
    28741 agaatcacca gcgccaccga gataatcagc gtttgctcga cttcttccag cgaggcgcga
    28801 atggtggggg agcgatcctg ggcaatttgc agatcaatcg ccgccggaat ggtctcctgc
    28861 aactctggta attttgcccg gatgctgtct accgtctgga taatattggc ttccggcagt
    28921 ttgcggatca tcagtaaaat agccggtttg gcgttggtca tcccggcgtt gcgcacatcc
    28981 tgcactgagt cggtcaccgt cgccacatcg cccagacgaa ccgcgccgcc gttgttgtag
    29041 tgaataatca gcggctgata ttcagcggcg gttttcagtt catcgttggt ctggatctgc
    29101 cagcggtgag tgccatcttc cagcgcccct tgcggtttac gcacgttggc attgctgatg
    29161 gcagtgcgta cgtcgtccag cgacacgccc tgattaaaca gcgcctgcgg gttcagcccg
    29221 acgcgcacgg cgggcagcga gctgccaccg acatcgacat caccgacacc gtcgatttgc
    29281 gaaatcgtcg gagccagctg cgtcgaggcg aaatcgtaca gttcaccctg cgaataagta
    29341 tcggatgtca gcgtgaggat cataattggc gcatccgacg ggttcgcttt gcgataggtc
    29401 gggcggctgg gcatcccgct gggcagcaaa ctttgtgcag cgttgatcgc cgcctgcaca
    29461 tcacgcgctg cgccgttgat atcccggtca aaatcaaact gcaaaataat gcgcgtgctg
    29521 ccgagcgaac tgctggaggt catttcacta accccggcaa tgcgcccaag tgagcgctcc
    29581 agcggcgtgg caacggaaga cgccattgtc tccggcgacg caccgggcag cgaggcgctg
    29641 accataatca ccggaaaatc gacctgcggc agcggggcga ccggcagcat acggaaaccc
    29701 agtatgccgc acagggtaat ggcgaccgac agtaaaatcg tcgccacagg gcggtaaatg
    29761 aagagggcaa aaaacttcac ttacgcctcc tcttcatggc gagcaaagcg acttttggtc
    29821 cacaatgcca ggcggtcgaa cagcagataa atcaccggcg tggtgaacag cgtcagcacc
    29881 tggctgacaa tcagaccgcc gaccatgccg atacctaacg gacgacgcag ttccgcgccg
    29941 accccggtac tcaacatcag cggcagcgcg ccaagcagag ccgccagggt ggtcatcagg
    30001 atcggacgaa aacgcaacag acaagcctgg tagattgcat cgcgcggcga catgccttgc
    30061 tcgcgctcag cagccagcgc gaagtcgatc atcatgatgg cgttcttctt cacaataccg
    30121 atcagcaaaa taatgccgat aatcgcaatc acatccagtt cgctaccggc aatcattaat
    30181 gccagcagcg cgccgacccc tgcggtgggt agcgtcgaga gaatggtgat cgggtgaata
    30241 aagctctcgt acagaatgcc gagcacgata tacatcgcca ccaccgccgc gacaatcagc
    30301 cagacagtgc tgcccagcgc cgactggaag gcgagggtgc tgccctggaa ttgcgtggtg
    30361 atatccaccg gcagattcag cgtcttttcg gtgtccataa tcgcctgcac cgcatcgccc
    30421 agcgaatagt tatccggcac gttaaaggag atggtcgtta ccgggaactg atccagatgg
    30481 ttgatggaaa gcggcgcaaa acgctgctca attttggcaa ttgagcttaa cggcaccacg
    30541 ccaccgtcgc tgctggtcag gcgaatggta tccagcgccg cgaggcctgg ggtattttcg
    30601 gtattgtgct ccagcaccac gcgatactgg ttagcctgag tataaatagt ggaaatcagt
    30661 cgctgaccaa acgcgttgta cagggcgtta tcgacgtccg ccatgctgat ccccagacgg
    30721 ctggcgctgt cgcgatcaac attgacatac gccaccagcc ctttgtcctg ccagtcgctg
    30781 gagacatcag aaagctgtgg cagttgctgg agtttttcca tcaactgtgg cacccaggta
    30841 ctgagcgcat ccagtgacgt ggcctgcaag gtaaactggt actgggtgcg gctgacctga
    30901 gtatcgatgg tcaggtcctg cgttggttgc aggaagagat cgacgcccgg cactttatct
    30961 accgccgttt gcagacgggc gatgactttt tgcacccgat catcacgttc atccaacggt
    31021 ttgaggttga tttgtaaacg tgcactattc agcgacgggt tagtgccatc aacgccaaca
    31081 aatgaggtca ggctttgcac tgccggatcc tgcaaaatca cgtccgcgac ctggcgttgt
    31141 cgctgggcca tattggcaaa ggagctggat tgcggtgcct gcaaagtgcc ctgaataatg
    31201 ccgttgtcct gtaccgggaa gaaacctttc ggaatgaaca cccacagcag cacgctaagc
    31261 agcagcgtgc tgagtgccac gcttaaggtc agccacggat gattcagcac tttcgccagt
    31321 ccacgaccat aggcggcgat tatcctgtcg aacatttttt ccgaggcacg ggagaagcgg
    31381 ttctgtttac gcaacgactc ctggctgagc atccgcgcgc acatcatcgg tgtcagggtc
    31441 agcgacacca ccgctgagat caaaatcgct accgccaggg taatagcaaa ttcgcggaac
    31501 agtcgcccga cgatatcgcc cataaacagc agtgggatca acaccgcaat cagtgagaag
    31561 gtcagcgaga taatggtaaa gccgatttca cctgcgccct tgagcgccgc cgccaacggt
    31621 ttttcgcctt tttcgatata gcgggaaatg ttttcgatca ccacgatggc gtcatcgacc
    31681 acgaatccgg tggcgatagt taacgccatc agtgtcaggt tattgattga aaaatcgaga
    31741 aacaccataa ccgcgaaagt gccgattaac gacagcggta cagcaacacc gggaatgatg
    31801 gtcgccggaa tattgcgcaa aaacaggtag ataatcatga ctaccagcgc gatagccatc
    31861 atcaattcaa actgagtatc atcgacggat gcgcggatat tggtggtgcg atcggaaagc
    31921 actgtcacct tcaccgattt cggcagactc tcagtgagct gtggcagcat ctgccgaatg
    31981 ctgtcggcgg tggagataat gttagcaccg ggctggcgct gaacattcat cacaatggcc
    32041 tgttctttgt tcgcccacgc gccgagccag ctgttttctg caccttgctc tacagttgcg
    32101 acatcgccca gacgaattgg cgcgccgttc tggtaggcga tgattagctg gcgatactct
    32161 tcggcggatt gcatctggtc gttcgcggaa agcgtgaccg cacgggaagg gccgtcgagg
    32221 ctaccttttg ccgagttaac gttagcgccg gtaatggcgg tgcgcacggt ttcgctggtc
    32281 aggccgaggg cggcaatcgc ctgagcgtta agtttgacgc gaacagccgg acgctgaccg
    32341 ccggaaagcg tcaccaggcc gacgccggaa atctgcgaga ttttctgcgc gacgcgggtt
    32401 tccaccatat cttccacttg cgtcatcggc atggcggttg aggtgacggc gagcgtcatg
    32461 atcggcggat ctgccgggtt cactttgctg taaaccggcg ggttaggcag atcgctcggc
    32521 aacaagttgg tcgcagcgtt aatcgcggcc tgcacttcct gctcggcgac attgagcggt
    32581 aatgttagct ggaactgcaa agtgataact gacgcaccgc cggaactttg cgacgacatc
    32641 tgtttcaggc cagacatctg cccgaactgg cgttcgagcg gcgcggtaac ggcagaggtc
    32701 atgacatccg ggctggcacc tgggtagagc gtgaccacct gaatggtcgg atagtccact
    32761 tccggcagcg ccgaaacggg cagggcgcga taaccgataa tcccggcgag taagatcgcc
    32821 accatcagca gcgtggtggc gacaggacgc ataataaaca ggcgcgacgg gccgcctgtg
    32881 ctgctcgggg gtaacacctg catcaggagc gtgctccttt tttcgcgtat tcgcggctgg
    32941 tggctttctc ttccggagtg gtggcgctct gggcttccac cacttccact ttcgcccctt
    33001 cggtcaggcg atcaatgccg tctgtcacca cgcgatcgcc cgcagaaata cctgcacgga
    33061 tcaccacttt ctgactgtcc tgaatgcccg gcgtcaccag atgtttgctg accttgtttt
    33121 cgctattcag cacccagaca aaatggcctt cattgcccat ttgcagggcg gctgttggga
    33181 tcactacggc gttttgttcg gtgtcgacta acatgcgcgc gttaacaaac tgattgggaa
    33241 acagcgcatc atcctgatta ttaaagcgtg ctttcacttt aatcgtaccg gttgtggcat
    33301 cgatttggtt atcgaggctt aacagcgtgc cttcacttaa tttcttcgaa ttggtgcgat
    33361 cccaggcttc taccatcagc ggttttccgg ctttttgcgc ctgcactaca gtagcgatat
    33421 cgctttccgg cagggtaaag actaaatcga taggatgcgt ctgggtgatc accacaattc
    33481 cggtggtatc accactggag atttggttac caacatcaac ttgcttgaga ccaacgcgac
    33541 catcgactgg tgcggtgatg cggctccagt cgagttgcaa ctgcgcgctg gcgacgcttg
    33601 cttcatcagc cttaatggtg ccttcggttt cactgaccag cgcctgttgg gcatccagct
    33661 cctgacggga gacgagattg gtttttgcca gttgctgata acgcgccaga tcacggcggg
    33721 cgttagcaag cgtggcttta tcttttgcca gttggccctg ggtttgtgct aatgcaactt
    33781 tgaactggct ggggtcaatt tctgccagta aatcgcctgc tttgacctgc tggccttcct
    33841 ggaaatgtaa cgccatcagt tgaccgtcca cgcggctgcg caccgtaaca gtattagcgg
    33901 cggtaatggt gccaagcccg gtgaggtaac gcggaactgc ctgttctacg gcggtcgccg
    33961 cctgaaccgg ggctaatggg ccggaacgca taccacggcg accacccgct ggcgattgct
    34021 gcgcttgttt cgtcgcccct ggggctgcac tccgggagtc attgcggcct tgccagaacc
    34081 agaatgcggc gatggcggcg ataaccacca cgattacgat tacccaacgg gatttataac
    34141 tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat actagtgtag
    34201 ccagccaata agggagaaaa atggaggaat tatgaaacac tatcgggaat cgtctgaatg
    34261 gcgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt atctacgcgt
    34321 tagcgataga ctgcatgcat ggcgaaagga ggtaagccgc cgatttcagc gggacgctga
    34381 aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat gatgaagcta
    34441 ctcatcatcg tggtgctctt agtcattagc tggccagctt actaagacta ccagggcggg
    34501 ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat tgtcaatctg
    34561 caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt acgcaataat
    34621 cacactggaa actattttat ccgcaagtca gcgatagact gcattcaggg cgaaaggagg
    34681 taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg aaggggcctt
    34741 aaataaggaa agggtcatga tgaagctact catcatcgtg gtgctcttag tcataagctt
    34801 ccccgcttac taagactacc agggcggggg aaaccccgct ctaccctcac tcctgaaatt
    34861 atgccttcac gataagattg tcaatccgta ggctttgtag tctgcgatcc tgccagcaaa
    34921 tattctttgc gagttgttac gcaataatca cagaggaaag tattttgttc acgcgttagc
    34981 gatagactgc attcagggcg aaaggaggta agccgacgat ttcagcggga cgctgacacg
    35041 ggaaagcccc tcccgaggaa ggggccttaa ataaggaaag ggttatgatg aagcacgtca
    35101 tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac c
//
LOCUS       DADLSE010000028        34770 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLSE010000028 DADLSE010000000
VERSION     DADLSE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.028"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(19..94)
                     /locus_tag="JN692_001358"
     tRNA            complement(19..94)
                     /locus_tag="JN692_001358"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="JN692_001359"
     tRNA            complement(153..229)
                     /locus_tag="JN692_001359"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="JN692_001360"
     CDS             complement(332..1717)
                     /locus_tag="JN692_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7263709.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN692_001361"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN692_001361"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7263710.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN692_001362"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN692_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7263711.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVHFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN692_001363"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN692_001363"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7263712.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEQ
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN692_001364"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN692_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7263713.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN692_001365"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(6328..7458)
                     /gene="rffA"
                     /locus_tag="JN692_001365"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HAY7263714.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN692_001366"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN692_001366"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7263715.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN692_001367"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN692_001367"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7263716.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIRKILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQCFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN692_001368"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN692_001368"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7263717.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQGERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN692_001369"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN692_001369"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7263718.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN692_001370"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN692_001370"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7263719.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN692_001371"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN692_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7263720.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN692_001372"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN692_001372"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7263721.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN692_001373"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN692_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7263722.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN692_001374"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN692_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7263723.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN692_001375"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN692_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7263724.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="JN692_001376"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="JN692_001376"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7263725.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="JN692_001377"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="JN692_001377"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7263726.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN692_001378"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN692_001378"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7263727.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            22012..22293
                     /gene="ppiC"
                     /locus_tag="JN692_001379"
     CDS             22012..22293
                     /gene="ppiC"
                     /locus_tag="JN692_001379"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7263728.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN692_001380"
     CDS             complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN692_001380"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7263729.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGAYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     CKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23958..24848
                     /gene="ilvY"
                     /locus_tag="JN692_001381"
     CDS             23958..24848
                     /gene="ilvY"
                     /locus_tag="JN692_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7263730.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JN692_001382"
     CDS             complement(24845..26389)
                     /gene="ilvA"
                     /locus_tag="JN692_001382"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7263731.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JN692_001383"
     CDS             complement(26392..28242)
                     /gene="ilvD"
                     /locus_tag="JN692_001383"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7263732.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JN692_001384"
     CDS             complement(28307..29236)
                     /gene="ilvE"
                     /locus_tag="JN692_001384"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7263733.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSLSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JN692_001385"
     CDS             complement(29256..29519)
                     /gene="ilvM"
                     /locus_tag="JN692_001385"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7263734.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29516..31162)
                     /locus_tag="JN692_001386"
     CDS             complement(29516..31162)
                     /locus_tag="JN692_001386"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7263735.1"
                     /translation="MNGAQWVVHALREQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMVEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31165..31215)
                     /locus_tag="JN692_001387"
     CDS             complement(31165..31215)
                     /locus_tag="JN692_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263736.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JN692_001388"
     CDS             complement(31301..31399)
                     /gene="ilvL"
                     /locus_tag="JN692_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7263737.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31751..33271
                     /locus_tag="JN692_001389"
                     /pseudo
     CDS             31751..33271
                     /locus_tag="JN692_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JN692_001390"
     CDS             complement(33296..33634)
                     /gene="maoP"
                     /locus_tag="JN692_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7263738.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33753..34592
                     /gene="hdfR"
                     /locus_tag="JN692_001391"
     CDS             33753..34592
                     /gene="hdfR"
                     /locus_tag="JN692_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7263739.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34689..34764)
                     /locus_tag="JN692_001392"
     tRNA            complement(34689..34764)
                     /locus_tag="JN692_001392"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34729..34731),aa:Trp,seq:cca)
BASE COUNT         8475 a         9516 c         8782 g         7997 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 gcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttaccgt
      361 ggcgattaag cccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccga
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tacgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ccggtacgcc gtgacgggaa acttttgcca ttgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgctgccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agagcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccaccgcgat caatgcgggt atccactgcg ccatctccgg gaaccaaaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag taagaccagg
     1441 cggtgagata gccaaaaaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaaa acgaacagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac gaagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcatgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gtacttccca ggtaaagtaa ttggctgagt
     3061 tcagcaccat actcgggcgc cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggtgaaaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta acgccagcca gaacatgcca acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggaaaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagtg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccgcc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcactggca aatgctgttc cgtcatatcc tgccagaacg gattttccct
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca gccccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc acgtaaagcg gcgacatgct cattgctgcg
     4501 gtcgccggag ttccccacca gaatggtcat ttttccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc tgttctcccc gcacctttgg
     4621 gtgcgttttg gcaaaaaagc tcaaatcgcc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcgcgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 gcggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgctg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt taatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgccc
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggtcc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcagctcgat acgcccggct ttcgccagag gcgctaacgc
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 catagccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcccccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccatgg cgctaaggcg tctgacgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aagtgctggc ctcaatcagg ctgtcgtgag tgccggtatc cagccaagca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc gcgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttcaagcg agatagcgcg gaaattgtcg tcaaattcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagccaaa aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agcactgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccattaagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcatatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga attttgcgga taccggccag catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtaccggaa ccgcccgcca ggataatacc tttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcac cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgaa ccgttttacg cattccactt
     9121 tcaaaggttt cctgcggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc
     9241 acgccgtgcg gcttgttcga agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgtgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcgc tcgccttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acaattcgct ggcgcgggcc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 atacacgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtcagatc cgggcgcatc tctgctaacc attctgccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtaggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcagt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actctcacgc ctatgaccgg tcaccagaat catcttttta tcggggtcga taaacgggta
    11881 atttgccgcc agttctgaac gcagcgtgtc gctgctcatc acctggtcac gcacccataa
    11941 cagtgcatca atgactgtat taccggtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 cgtcgtatcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagtacctga tcaagcatct cccgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggctcgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gagtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacgatcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagctctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcgg cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcaacagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcagttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccacgccg
    17281 acgcggaacc gctggcggaa gctaccggcc tgaagctggg cctggcttac ggtggtgatg
    17341 gctatgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcaccacgg
    17401 gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtagtgg
    17461 tactggacga agccgatcgc atgtacgatc taggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aaccagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc
    18181 gcacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcttact gtagtaagtg attcgggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaatt
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgacctctc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgacttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcgcgtctga tttatcaggg tgttgctcac accactggtg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcaag aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacact gcgtaatatt
    19321 caacgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa cgaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact tcaaacaagc tccgcaacac
    19501 gctgcttatc tggtgcgtaa cctggatctt cccggtttta cccccgcaca gaagaaactg
    19561 ctggcgacgc tactgctcaa ccagactaac ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggctatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaaaccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tctccggcct tgattatttc cctcgttttg
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cactgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagttcgc cgtactggcg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatactg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg cgtggagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgtaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcagcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtgaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaactttt agctctgtgc
    21001 cgtaacccag ttcggagaac agacgctttt caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctgg ctcagcagca ccaggttttg cggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcatcg tcacccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ttcgtccacc agcaggtagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagaagt agcgtcggca
    21361 ataaaatcag atcatcgaag tcgagcacat tacaggcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcac ccgcggatca gatgggcgat tttattggtg ataacacgag
    21841 ttttaccgga acccgcaccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggaa aatcgctcaa tgaaagtcgg gaggggaaaa
    21961 taacagcgtg gtagtatagc cagcctcatc cttaccactc aaggcacgat catggcaaaa
    22021 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga tcttctcgtg
    22081 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    22141 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    22201 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    22261 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaaggcc
    22321 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    22381 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    22441 cagctgcgcg ttatctaccg caccttccgg aatagcttta cccaaatcgc ccggttgcag
    22501 ttctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    22561 ctcggcagta tcagagataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    22621 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gattcttcga tgatgccgga
    22681 atcaaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga tcatcagtac
    22741 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcgaa
    22801 ggcggttttg caggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    22861 cgccatcata ccggaggaga attcgccgga gatgatgtcg tccatatgtt tctggaacag
    22921 cggtgccatg atctctttca gctgttcaga caacgcgtaa gcacgcagtt tcgccgggtt
    22981 agagagacgg tccatcatca gtgtgatacc gccctgcttc agcgcttcgg tgatggtttc
    23041 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    23101 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    23161 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    23221 ggttgctgcc gcccatgctt tagcgatcgc catgccttcg cctttcggat cgttttccgg
    23281 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    23341 gccagggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    23401 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    23461 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    23521 ctgtgggatc agttcttcgt aagcacccac tttaaaacca ttttcggtcg ctttacgcca
    23581 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    23641 atcacgcatg ttcagaccct ggttcagacc ttgtgcgcca cagccgacga tgactacttt
    23701 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    23761 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    23821 tcctcgtgat gttgtgcttc ttattgttgc gtttgcgaga ttgaattcac tatatgacag
    23881 gaaatttatt gcggaaattg atatattcac aacgtcatat tgcaatttct gcaacgtcaa
    23941 cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    24001 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg ctctcacggc
    24061 agattcagcg cctggaagaa gatctcggcc agccgctgtt tgtgcgcgat aaccgcacgg
    24121 tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc ccagcaaacg ctgttgcagt
    24181 atcagcagct gcgccacacc atcgaccagc aagggccgtc gctctatggc gaattacata
    24241 tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc gattctggat cgcttccgcg
    24301 cggaacaccc gtcggtggag atcaaactca ctaccggtga tgcggcagat gcaatggaaa
    24361 aagtcgtcac aggtgaagct gatctggcga ttgccgggaa gccagagact ctgcccggcg
    24421 cggtggcgtt ttcgatgctg gagaatctgg cagtggtgtt gattgcaccc gcattgccct
    24481 gcccggtgcg taatcaggtt tctgtcgaaa agccggactg gtcaacggta ccgtttatca
    24541 tggccgatca ggggccggtg cgccgccgca ttgagttgtg gttccgacgc aataaaatca
    24601 gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    24661 tcggctgtgg cgtggcattg ttgccggaag tggtgctgga aaacagcccc gaaccggtgc
    24721 gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac gccgtttgag ctgggcgtgt
    24781 gtgcgcagaa aaagcggcta catgagccgc taattgaggc attctggaag attttgccga
    24841 accattaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt
    24901 agcccagctc attcagccgg gtttcgaaat ccggttcatg gtcgccaagt tcgaacgccg
    24961 ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg gaacaaagaa atgttccagt
    25021 gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg tgattccggg aattcgaagc
    25081 tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc gaccatatag cgcacatgca
    25141 acttcgccat ttcgtcgtcg gagagatcca ccacgctgta gccgccgtcg ttgagcatct
    25201 gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg cacgccgaca aagatgcagg
    25261 cgtttttggc atcggcaaaa cggtagttga actcggtgac cgatcgcccg ccaagcagtt
    25321 ggcagaattt gaggaagctg cctttttctt ccggaatggt caccgccaac aacgcttcac
    25381 gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag gccgtggaag ttcacgttgg
    25441 caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg atatattttt
    25501 tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca tcttcgaata
    25561 aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg tcgagatact
    25621 cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgcgacacct tcggcgaaca
    25681 gtcccacgcg tggcagatca accggatgac ccgcatccag cgctgctttc aggcaggcgg
    25741 agtcttccgc ttctacggcg atcactttga tttgcggcat cagttgtttg atcagcaccg
    25801 ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg agatgggcgt
    25861 cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc ggatgatcga
    25921 acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta gctttcgctt
    25981 cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc gcatcgactt
    26041 tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac cgcgcagaag
    26101 aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg tgcgcttttt
    26161 gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag ctgtgcactg
    26221 gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac agtttttcca
    26281 ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc actgctctta
    26341 aatattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt attaaccccc
    26401 cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc tggcgtaggc
    26461 gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt tgtcaccccg
    26521 agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatgcc
    26581 acggttcggg atgtcgatag cgatcaggtc accatcttca atcagaccaa tgctgccgcc
    26641 gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc cagagaaacg
    26701 accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga agctggttgg
    26761 gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga tcaccaccac
    26821 atcgcccgcg acaactttgc caccgaggat cgcttctacc gcgtcgtcct ggctttcgta
    26881 cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg ttttcacgat
    26941 gcagccgttt tcagcaaaat taccgtagag caccgccagg ccgccgtctt tgctgtaggc
    27001 gtgttccagc gagcggatac agccgtttgc gcgatcgtca tcgagagaat cccaacggca
    27061 atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg cctgcgcgga acatattttt
    27121 taccgcgtca tcctgggtca gcataacgtc gtattgttcc agagtttgcg gcaacgtcag
    27181 gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt caccgagaat
    27241 accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttttggg tgctcggcgc
    27301 aactttgcat aactgcggga ctttacgcga aagtttgtcg atatcactca tggtgaagtc
    27361 gatttccgct tcctgcgccg ccgccagcag gtgaagtacc gtgttggtag agccgcccat
    27421 cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg ctagcaatat tgcgcggcag
    27481 tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc gtttaccggc
    27541 attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt tgcccggctg
    27601 cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca tcccggagca
    27661 ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt cagaaacttt
    27721 cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct gatcggacag
    27781 tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg
    27841 cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc agaccatggc
    27901 gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt cgcgagatgg
    27961 aagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccaccg caatggtgtt
    28021 gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt taccgagatc
    28081 gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg
    28141 cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat
    28201 attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt
    28261 gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt
    28321 gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag aaggcttgct
    28381 gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat ctgaataccg tctacgctgc
    28441 gcactggcgt gatttctgcc gccgtaccgg acataaagac ttcatccgcc aggtacaggg
    28501 attcgcgcga cagcacctgc tcacgcactt caattcccag ctctttcgcc agtttgatga
    28561 tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg aacagcacac
    28621 catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg ttcacatcca
    28681 gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc agggaagaga
    28741 gatagttacc accggctttt gccgcagttg ggatggtgtt tggtgctgcg cggttccagg
    28801 aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gctccccacg
    28861 ggaaagcggc gataatcaca tcggttgagt atcccgctgg cgggtttact cccatgccaa
    28921 catcaccgac gaagatcagc ggacggatat aggcgctggt gagattgttt ttgcggatca
    28981 cgtcacgaca agcttccatc agctcatcaa tgctcagtga aaccgggaag cgatagattt
    29041 tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt
    29101 gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc atagtgcagc gcgtgcgaca
    29161 tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag
    29221 ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgtg
    29281 gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac
    29341 agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg
    29401 ctggcggcag ccatattcat tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa
    29461 acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg
    29521 ataatttctc caacatttct gaattactgg cacctggcgg caccagcggc cagacgttct
    29581 caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga
    29641 gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg aaggcgctgg
    29701 ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc gctgtatcgt tcctgaaaaa
    29761 acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca
    29821 acggtaactg cttgcgtttt acggtgccca gctcttgcac attcatcatg aaagagccgt
    29881 caccggagat acagacaacg gtatcgttcg gtcgcgcgac ttgtgcgcca actgccgccg
    29941 gtaaaccaaa acccatggta cctaagccgc tggaggtgat gaaattttcc gggcgagtgt
    30001 gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc acgacgcaat
    30061 ccgcaggttt acgatccgac agttgtttta acaacaacgg agcgtagata gcgtcaccgg
    30121 gatggtcgta acgccaggaa tgttcatcac gcagctgcgc gcagtgttgc tgccagtcat
    30181 tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct tgtaatgcca
    30241 catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg ataacactgg
    30301 cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcacccacgg
    30361 cgatcagcag gtcacactcc tgcaccgcga agtttgccgc tttggttccg tgcatcccca
    30421 gcatgcccag atagtacgga taatctgctt ctactgcgcc cagccctttc agcgtacagg
    30481 tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc cggaactgcc tgcgccatcc
    30541 ccacgccacc gccaacgtac agcatcggtt tttgcgcttt tgccagcatc tggcgcgctt
    30601 gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac cacggttcca
    30661 gatcgccgct ggctaactgg atatcttttg ggatatcgac cagaaccgga ccaggacgac
    30721 ctgagctggc aacgtcgaat gcttcaacca tgatgcgcgg caactcttcc agagactgca
    30781 ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact tcctgaaatg
    30841 cgtcggtgcc gataaacggt gcggacacct gaccagtgat ggcaacgaca gggatggaat
    30901 ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca gacgtggcga
    30961 tacatacgcc agttttaccg gtagcacggg cataaccgat agccgccatt gccgcaccct
    31021 gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg taaaccggca
    31081 taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgttcccgc aacgcatgta
    31141 ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt
    31201 tgtgctgtta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa aacccccgga
    31261 cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt cctcgtccaa
    31321 gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc aggctaatca
    31381 ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga
    31441 gttaccatag attttgcgaa tagcacaaga tatttttggg cctattttta tgatggcttt
    31501 aataatttca atagaaattg ttgtttttta aaaagaaaat agtgagtgaa aaaattcaat
    31561 ttcagtataa atgttgtccc cttcctcgta gttcatcctt tgtccgctgc atttttgcga
    31621 gcatcattcc accgccagaa caactcaact tcacttgcac aaataaacag gaatcagcca
    31681 ctaaaattca cgaattagcg ggctacagca gcgatatctt atgcacataa tggtgactca
    31741 aggagggttt atgtcactgt caattgttca tacccgcgca gccctggaag taaatgcgcc
    31801 cccaatcact gttgaggtac atatcagtaa aggtctaccc ggcttaacga tggtgggctt
    31861 accagaaaca acggtgaaag aagctcgcga tcgcgtgcgc agcgccatta tcaatagcgg
    31921 atatgaatat ccggcgaaaa aaatcaccat caaccttgct ccagccgatc tgccaaaaga
    31981 agggggacga tatgatttac ctatcgccat tgcgttgctg gcggcctcag aacagcttac
    32041 agccaataag ttagatgaat atgaattagt cggagaacta gcgcttacag gcgctctgcg
    32101 tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt aagtcgggca gaaaaattat
    32161 cgtcgcgaaa gataacgaag atgaagtggg gctaattaac ggtgaaggat gcctgatagc
    32221 cgatcatctg caggctgtct gtgcgtttct ggaaggtaag aacactctcg aacgcccgaa
    32281 accaactgat gcagtatccc gggcgctaca acatgatctc agtgatgttg tcggtcagga
    32341 gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg cacaaccttt tactgattgg
    32401 gccgccggga acaggtaaaa caatgctcgc cagccgtatt aatggcctgt tgccagattt
    32461 aagcaatgaa gaggcactgg agagcgctgc gatattaagt ctggtaaatg ctgaatcagt
    32521 acaaaaacaa tggcggcagc gcccgttccg ctcacctcat cacagtgcgt cgttaactgc
    32581 gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt tcgttggcgc ataacggcgt
    32641 gctttttctt gatgagctac ctgaatttga acggcgtaca ctggatgcct tgcgagagcc
    32701 gattgaatcc gggcagatcc atctttcacg cacacgagca aaaataacct atccagcccg
    32761 tttccagctt gttgcggcga tgaatcccag ccctaccgga cattatcagg gaaaccataa
    32821 ccgctgcacg ccagaacaga cattacgtta tctcaaccgg ctctcggggc cctttctcga
    32881 ccgcttcgat ctctcactgg agatcccatt accacccccc ggcattttga gtaaaacggt
    32941 agtgccggga gaaagcagcg ccaccgttaa acaacgcgta atggccgcca gagagcgcca
    33001 atttaagcgg cagaataaac tgaatgcctg gctggatagt ccggaaatac gccaattctg
    33061 caaacttgag agcaaagatg cgatgtggtt ggaagaaacg ctgatccatc tggggttatc
    33121 gattcgtgcc tagcagcggt tactgaaagt tgcacgaacc attgctgata ttgatcagtc
    33181 tgacattatc acacgtcagc atttgcagga ggcagttagc tatcgtgcga ttgaccgttt
    33241 gctcatccat ctgcaaaaac tactgacata aaaaaagggc atttcgccct ttttattaat
    33301 cgtcagaatc ggtgtagtct tcagcacctt caacctgcgg tttaccgccg gaaagggtgt
    33361 gaaaacgttt tggacgctta atacgcgtca tatacttgga ccacacgcgt tctgcttctg
    33421 tcactggctc acgttcgcca cggcatactg ctacgaagag tttctcttcc tcggtaaccg
    33481 gctcgcgttt gccaagatcc aactcattga aggcataacc atgacgctca agcagttgtg
    33541 cctctttgat ggtgaaatca ccatgacgag agaatccacg tggataatgt ttattgtcga
    33601 aatatcgatt agtcgtcgta aagctttccg ccatcctgca cgctcctaat tctttgaccg
    33661 agctagttat ggcgcggagt attagttacg cttgacagag tgtaaaacaa aacatttaaa
    33721 tcataacgac aaataatttt gcggagagca ctgtggatac ggaattgtta aaaactttcc
    33781 tggaagttag ccgaacgcgt cactttggtc gagcggctga atcgctctat ctgacccagt
    33841 cagcagtgag ctttcgaatc agacaactgg aaaatcaact gggtgtgaac cttttcaccc
    33901 gccacagaaa caatatccgt ttaaccgctg ccggtgaaaa actactgcct tatgcagaaa
    33961 cgctcatgag cacctggcag gccgcccgca aggaggtggc gcatacctca cgacataacg
    34021 agttttctat cggtgccagc gcctcgttgt gggaatgtat gcttaatcag tggttgggac
    34081 gcttgtatca aaatcaggat gcccatacag gcttacagtt cgaagcgcga attgcccaac
    34141 ggcagtctct ggtaaaacag ctgcatgaac gccagcttga tcttcttatt accactgaag
    34201 cgcccaaaat ggacgaattt agtagtcagt tgctggggta tttcacttta gcgctttata
    34261 ccagtgcccc ttctaaacta aagggagatc ttaactatct gcgactggag tgggggccag
    34321 attttcaaca gcatgaggca ggtttgatcg gtgctgacga agttcccatt ctgacaacca
    34381 gttctgctga actggcacag caacagattg cgatgcttaa tggttgcacc tggctacccg
    34441 tcagctgggc gcgtaaaaaa ggcggcctgc ataccgttgt cgatagcaca acactttcac
    34501 ggccgcttta tgccatatgg ctgcaaaata gcgataaaaa tgcgttgatt cgcgatcttt
    34561 tgaaaattaa cgtgctggat gaagtgtatt aatctgaatg gctggcaagg atgccggtag
    34621 aaggatttac ttcggagagg attatttcag ataaaaaaaa atccttagct ttcgctaagg
    34681 atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg gagaccggtg
    34741 ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DADLSE010000029        34363 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLSE010000029 DADLSE010000000
VERSION     DADLSE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.029"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..329)
                     /locus_tag="JN692_001393"
     CDS             complement(<1..329)
                     /locus_tag="JN692_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263740.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYLQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
     gene            699..1649
                     /gene="corA"
                     /locus_tag="JN692_001394"
     CDS             699..1649
                     /gene="corA"
                     /locus_tag="JN692_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7263741.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1690..2070)
                     /locus_tag="JN692_001395"
     CDS             complement(1690..2070)
                     /locus_tag="JN692_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7263742.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2084..2500)
                     /locus_tag="JN692_001396"
                     /pseudo
     CDS             complement(2084..2500)
                     /locus_tag="JN692_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN692_001397"
     CDS             complement(2559..3449)
                     /gene="rarD"
                     /locus_tag="JN692_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7263743.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3501..3968)
                     /locus_tag="JN692_001398"
     CDS             complement(3501..3968)
                     /locus_tag="JN692_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7263744.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASVLDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4133..5002
                     /gene="pldA"
                     /locus_tag="JN692_001399"
     CDS             4133..5002
                     /gene="pldA"
                     /locus_tag="JN692_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7263745.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5135..6964
                     /gene="recQ"
                     /locus_tag="JN692_001400"
     CDS             5135..6964
                     /gene="recQ"
                     /locus_tag="JN692_001400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7263746.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7028..7648
                     /gene="rhtC"
                     /locus_tag="JN692_001401"
     CDS             7028..7648
                     /gene="rhtC"
                     /locus_tag="JN692_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HAY7263747.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN692_001402"
     CDS             complement(7710..8330)
                     /gene="rhtB"
                     /locus_tag="JN692_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7263748.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8441..9463
                     /gene="pldB"
                     /locus_tag="JN692_001403"
     CDS             8441..9463
                     /gene="pldB"
                     /locus_tag="JN692_001403"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7263749.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNIRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9471..10271
                     /gene="yigL"
                     /locus_tag="JN692_001404"
     CDS             9471..10271
                     /gene="yigL"
                     /locus_tag="JN692_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7263750.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMCFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10347..11246
                     /gene="bioP"
                     /locus_tag="JN692_001405"
     CDS             10347..11246
                     /gene="bioP"
                     /locus_tag="JN692_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7263751.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN692_001406"
     CDS             complement(11134..12087)
                     /gene="metR"
                     /locus_tag="JN692_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7263752.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNCPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12323..14584
                     /gene="metE"
                     /locus_tag="JN692_001407"
     CDS             12323..14584
                     /gene="metE"
                     /locus_tag="JN692_001407"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7263753.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14623..15438)
                     /locus_tag="JN692_001408"
     CDS             complement(14623..15438)
                     /locus_tag="JN692_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7263754.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYSGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15700..16461
                     /gene="udp"
                     /locus_tag="JN692_001409"
     CDS             15700..16461
                     /gene="udp"
                     /locus_tag="JN692_001409"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7263755.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16602..18029
                     /gene="rmuC"
                     /locus_tag="JN692_001410"
     CDS             16602..18029
                     /gene="rmuC"
                     /locus_tag="JN692_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7263756.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18124..18879
                     /gene="ubiE"
                     /locus_tag="JN692_001411"
     CDS             18124..18879
                     /gene="ubiE"
                     /locus_tag="JN692_001411"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7263757.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN692_001412"
     CDS             18893..19498
                     /gene="ubiJ"
                     /locus_tag="JN692_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7263758.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19495..21135
                     /gene="ubiB"
                     /locus_tag="JN692_001413"
     CDS             19495..21135
                     /gene="ubiB"
                     /locus_tag="JN692_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7263759.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21214..21483
                     /gene="tatA"
                     /locus_tag="JN692_001414"
     CDS             21214..21483
                     /gene="tatA"
                     /locus_tag="JN692_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7263760.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21487..21987
                     /gene="tatB"
                     /locus_tag="JN692_001415"
     CDS             21487..21987
                     /gene="tatB"
                     /locus_tag="JN692_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7263761.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPEKASDE
                     AHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKTAAPSP
                     SSSDKP"
     gene            21990..22766
                     /gene="tatC"
                     /locus_tag="JN692_001416"
     CDS             21990..22766
                     /gene="tatC"
                     /locus_tag="JN692_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7263762.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22808..23590
                     /gene="tatD"
                     /locus_tag="JN692_001417"
     CDS             22808..23590
                     /gene="tatD"
                     /locus_tag="JN692_001417"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7263763.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN692_001418"
     CDS             complement(23587..24075)
                     /gene="rfaH"
                     /locus_tag="JN692_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7263764.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24242..25735
                     /gene="ubiD"
                     /locus_tag="JN692_001419"
     CDS             24242..25735
                     /gene="ubiD"
                     /locus_tag="JN692_001419"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7263765.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25781..26482
                     /gene="fre"
                     /locus_tag="JN692_001420"
     CDS             25781..26482
                     /gene="fre"
                     /locus_tag="JN692_001420"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7263766.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEADWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN692_001421"
     CDS             complement(26674..27837)
                     /gene="fadA"
                     /locus_tag="JN692_001421"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7263767.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWSCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGMGQGIATVFERV"
     gene            complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN692_001422"
     CDS             complement(27847..30036)
                     /gene="fadB"
                     /locus_tag="JN692_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7263768.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30226..31557
                     /gene="pepQ"
                     /locus_tag="JN692_001423"
     CDS             30226..31557
                     /gene="pepQ"
                     /locus_tag="JN692_001423"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7263769.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31557..32171
                     /locus_tag="JN692_001424"
     CDS             31557..32171
                     /locus_tag="JN692_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7263770.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32210..33661
                     /gene="trkH"
                     /locus_tag="JN692_001425"
     CDS             32210..33661
                     /gene="trkH"
                     /locus_tag="JN692_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7263771.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAIDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33673..34218
                     /gene="hemG"
                     /locus_tag="JN692_001426"
     CDS             33673..34218
                     /gene="hemG"
                     /locus_tag="JN692_001426"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7263772.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8075 a         8696 c         9290 g         8302 t
ORIGIN      
        1 tgctgaccgt tttcgatgta caaaccgagc ggcgcatagc tttcatcata gatgccgccg
       61 ttcatcgcca tctgcacctg accctgacta ttaatatccg ccagcagagc atgtaacgtt
      121 ccccacgctt cgccattggc tttttgcaag tacattttca cccgctctgt ttgaggatta
      181 acggtatacg cctgtacggt cagcgtcgga tctgagagtg cgcaatcatc agcggcaaca
      241 gcaaacaagg gaagtaaggt gagggtgaga aaaatccgtt tgagattcaa ggtgatcatt
      301 cctttaccaa tgagtagccg atgcgccatt ataggtcctg gatgtgggat ttttttatcc
      361 tgttagcgac cttgacgagt accaaaaagc gcgaagttca actattgttc tgtggtgttc
      421 tgttgcgtgt tgacggcaaa attttgctgg cgtaacatgc gcgcacgatc actctaagag
      481 gacattcgcc ttggacacac ccagtagata ctggctcact atcctgtcat ccaggatcaa
      541 ctcctaaggc tatccctttt tgctgatagc cttagcggtt gtcagcgacc tcaatttttc
      601 ccgtcgcgct gagtcaggct gtttaatggt ctgaaaccca atttgtttct gtgtgcccac
      661 cgaactgtcc gatattttaa gcattgggag tcccggtcat gctgagcgca tttcaactgg
      721 aaaataaccg actgacccgg ctggaagtcg aagagtcaca accccttgta aatgcagtat
      781 ggattgatct tgtcgaaccg gacgacgacg agcgactgcg cgtacaatct gaacttggcc
      841 agagcctggc aacccgcccg gaactggaag acatcgaagc atcggcacgt ttctttgaag
      901 acgacgacgg cctgcatatt cactccttct tcttctttga agatgcggaa gatcacgccg
      961 gtaactccac tgtggcattt accatccgtg atggtcgtct gtttactctg cgtgagcgtg
     1021 aactgcccgc ttttcgtctg tatcgtatgc gtgcccgtag ccagtcgatg gtagacggta
     1081 acgcctacga gttgctgctg gatctgttcg aaaccaaaat cgaacagttg gcagatgaaa
     1141 ttgaaaatat ctatagcgac ctggagcagt tgagccgggt gattatggaa gggcatcagg
     1201 gcgatgagta cgacgaggcg ctctccacgc tggcggaact ggaagatatc ggctggaaag
     1261 ttcgcctgtg tctgatggat acccagcgcg ctctcaactt cctagtgcgt aaagcgcgtt
     1321 taccgggtgg gcaactggag caggcgcgtg aaatcctgcg cgatatcgaa tccctgctgc
     1381 cgcataacga atccctgttc cagaaggtga acttcctgat gcaggcggca atgggtttta
     1441 tcaacatcga gcagaaccgc atcatcaaaa ttttctcggt ggtatccgtc gtgttcctgc
     1501 cgccaacgct ggttgcttcc agctacggga tgaactttga gtttatgcca gaactgaagt
     1561 ggagtttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg gcaccgtatc
     1621 tgtactttaa gcggaagaac tggttgtaaa aagaaggcgg tggcttagcc aggctaagcc
     1681 tcctgttatt caaaggctcc aggtatttaa cccttttacc tctttctcat aaaaccattt
     1741 gttcgtgtta acagcaacat aggttgctac ggcaattccc agaatgttaa cgccaattaa
     1801 tgcaccaaca aatagagata gaatgccaag taatagaact ataattgctt ttttccataa
     1861 gcccagaaca aataaataga tccaggaaca gaagaaagca atgaaattca tttgaatagt
     1921 taatcgttgt cttactttta atgctttaaa tgctgcttta tattctggtg ttgctcccca
     1981 gaatccagga aagccatgtt gatcataaaa attaaatcgg tatttccatt tttcacttaa
     2041 tgaatcatcg ttcatatatt ccttactcat aaatactcca taactattgt tttgatgaat
     2101 cagtaggtgc aagcattagc atactgaaag tggaaaaata acaaatcaaa aaaatcatcg
     2161 aaccattgcc tgaacaggca aaatcttcgg ctatcattgt gatgatagag atgatatata
     2221 ctgctaatgt accaaaaaca taaatttata tatatatgaa accactatca cggagtcgtt
     2281 ggcaattcat gttgatgacg agataatgga gtgcgatggt agagactata acaagaaaac
     2341 ctgcttcccc atcgttaaaa aagataataa gaaaggcaaa aatgaaattt attaaaataa
     2401 atgaaaatat atatcggcgt ctggaaatct taccgttaga tgttgggata aacatacgta
     2461 acataaattt tacatccttg tgtgagtctc ctgtcagcat ggcaatatgc ccactctcat
     2521 acaagagtcg gcatattttt tcagaatata tttatttttt atttggacgt tctacgctgc
     2581 gtataaatcg cgtccatcac aaaaattgcc agcgccaccc aaataaaggt gaaagtcacc
     2641 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
     2701 gggccaatgt actgaaaaaa gcctaacgtt gagagacgca ggcgcgtggc agcggcagta
     2761 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
     2821 atcgggtttt gccccatatg gctggttgaa ctgtcggcaa tagcaaacag gtaaatcgcc
     2881 gccacgggca gcagccacat ggtttcaatt aacatgccgg tttgtgcttc taccgcaatc
     2941 ttcttgcgca ccaggccgta gaaggcaaaa ctaaatgcca gccccagcgc gataataggt
     3001 agcgaaccaa aagtccacag ctggaccaac acgccacata tcgccagaat caccgccagc
     3061 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     3121 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     3181 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     3241 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     3301 ataaagaaaa acgaccagat cacgcgatgc gtcaggatct catcggcggg cacgtagtaa
     3361 atcagcttga agtatgctgg cgcaataccc caaataaaat aagcggcaag agcgagtaat
     3421 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     3481 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     3541 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     3601 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     3661 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     3721 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcac cgacgcaatg
     3781 actccgccgt gcaaaatgct ttgcgcccag ttacccacca tcatcggctg atttttaaag
     3841 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     3901 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     3961 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     4021 tgttggtttc tactttagga agggataact aacggcaatg gagttaagta tgtaaaatag
     4081 cccgcagaaa aacattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     4141 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     4201 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     4261 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatctacac
     4321 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     4381 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     4441 cccaaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     4501 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     4561 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     4621 ctctaacggg cgttcagacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     4681 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     4741 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     4801 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     4861 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     4921 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggggt
     4981 tatgctaaac gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     5041 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     5101 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     5161 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     5221 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggttg ttatgcccac
     5281 tggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     5341 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactac aggccaacgg
     5401 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     5461 aggctgccgc accgggcaaa ttcgcttact gtatatcgcc ccggaacgcc tgatgctgga
     5521 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgtcg atgaagcgca
     5581 ctgtatctca caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     5641 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     5701 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     5761 ccgtccgaat attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg
     5821 ctacgtgcag gaacagcgcg gtaagtctgg cattatctac tgcaacagcc gggcgaaagt
     5881 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     5941 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg atctgcaaat
     6001 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     6061 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     6121 tgatggcctg cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg
     6181 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgagcgcc acaaactcaa
     6241 tgcgatgggc gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt
     6301 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     6361 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     6421 gtttgggatg ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga
     6481 ctatggtcat gacaaactga aagtctatgg tatgggccgt gataaaagcc atgaacattg
     6541 ggtgagcgtg atccgccagc tgattcatct cggcctggtg acgcaaaata ttgcccagca
     6601 ttctgcccta caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca
     6661 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     6721 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     6781 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     6841 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     6901 acgctttggt aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga
     6961 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     7021 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     7081 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     7141 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgttgct
     7201 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg
     7261 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     7321 tgtatctgcg cctgcaccac aggttgagct ggcgaaaagc gggcgcagtt tcctgaaagg
     7381 cttactgacc aatctcgcca atccgaaagc gattatctat tttggttcgg tattctcatt
     7441 gtttgtcggc gataacgttg gcactaccga gcgctggggc attttcgcgt tgattattgt
     7501 cgaaacactg gcgtggttta ccgtcgttgc cagtctgttt gccctgccgc aaatgcgccg
     7561 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     7621 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     7681 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     7741 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     7801 ttaatccata gagcaatcct ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     7861 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     7921 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     7981 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaagatca
     8041 attgcgccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     8101 cactttaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     8161 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     8221 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     8281 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     8341 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgctt
     8401 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     8461 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     8521 gcgccagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     8581 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     8641 cgtaaaatat gcggaactgg cctacgacct gttccatttg gggtttgatg tcttaatcat
     8701 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     8761 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     8821 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     8881 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     8941 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     9001 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     9061 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacatacgct tctatgctga
     9121 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     9181 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttacaagc
     9241 tgaagaggaa cgcgtggtgg ataaccgcat gcacgaccgt ttttgtgaac tccgcaccgc
     9301 cgcgggccat cctgtcgaag gaggacggcc gttggtaata aaaggtgctt accatgagat
     9361 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     9421 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     9481 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     9541 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     9601 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     9661 cctccaatgg tgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg
     9721 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     9781 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgtgctttt
     9841 ttaaagaagc ggtgttcaaa tatgcgctgt atgagccggg attactggag ccggaaggcg
     9901 tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     9961 ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
    10021 tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct
    10081 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
    10141 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagat ctgcatccgg
    10201 agctggaagt tattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
    10261 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
    10321 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
    10381 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
    10441 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
    10501 atagcctgaa aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt
    10561 atatgctgag tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg
    10621 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
    10681 ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc
    10741 aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
    10801 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
    10861 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
    10921 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
    10981 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
    11041 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
    11101 tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct
    11161 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
    11221 ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc
    11281 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
    11341 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
    11401 ggcaaccatt tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
    11461 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa
    11521 tagcgtctcg ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc
    11581 aggtgctaac accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg
    11641 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
    11701 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc gggcagttct tatggaaatt
    11761 ttctaaagcg ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
    11821 cgtctgctgc ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt
    11881 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
    11941 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
    12001 cgtctgatgc aacgtcgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag
    12061 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
    12121 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc
    12181 cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca
    12241 gtaagctccc ggcgatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt
    12301 acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt
    12361 tggcctgcgt cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg
    12421 tgaagaactg ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca
    12481 agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac
    12541 cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat
    12601 cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc
    12661 ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg
    12721 ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct
    12781 gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt
    12841 gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca
    12901 agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct
    12961 ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca
    13021 gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac
    13081 gattactgcg ctgcctgttc aggggctgca tgtggacctt gtacatggta aagatgacgt
    13141 tgttgaactg cacaagcgcc tgccttctga ctggctgctg tctgcgggtc tgatcaatgg
    13201 tcgtaacgtc tggcgcgccg atcttactga gaaatatgcg caaattaagg acattgtcgg
    13261 caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag
    13321 cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg
    13381 ccatgaactg gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga
    13441 gtggagcgcc ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga
    13501 aaagcgtctg gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg
    13561 tgctgaagcc cagcgtgcgc gttttaaact gccagcgtgg ccgaccacca cgattggttc
    13621 cttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga
    13681 cgccaacaac taccgcacgg gcattgcgga acatatcaag caggccattg ttgagcagga
    13741 acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata
    13801 ctttggcgag cacctcgacg gatttgtctt tacgcaaaac ggttgggtac agagctacgg
    13861 ttcccgctgc gtgaagccac cgattgtcat tggtgacatt agccgcccgg caccgattac
    13921 cgtggagtgg gcgaagtatg cgcaatcgct gaccgacaaa ccggtgaaag ggatgctgac
    13981 ggggccggtg accatactct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat
    14041 cgccaaacag attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat
    14101 tggcatcatc cagattgacg aaccggcgct gcgcgaaggt ttaccgctgc gtcgtagcga
    14161 ctgggatgcg tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa
    14221 agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc
    14281 gattgcggcg ctggacgcag acgtcatcac catcgaaacc tcgcgttccg acatggagtt
    14341 gctggagtcg tttgaagagt ttgattatcc aaatgaaatc ggtcctggcg tctatgacat
    14401 tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa
    14461 acgcattccg gcagagcgcc tgtgggtcaa cccggactgt ggcctgaaaa cgcgcggctg
    14521 gccagaaacc cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg
    14581 gtaaatccaa accgggtggt aataccaccc ggtcttttct cattacagcg acttcttccc
    14641 accatactgc ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg
    14701 atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc
    14761 tgctttcgca ttagcagccc gcagcgcctg gcgcatcgtt tcaacgctct cctgcggaat
    14821 gctgttatcc tgaccactat ataagccgag aaccggcgcg ttaagatcgg ttgcgatatc
    14881 aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac
    14941 tgcggctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca
    15001 gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcgac
    15061 atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa
    15121 cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat
    15181 agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc
    15241 aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc
    15301 atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc
    15361 gtcgtccggg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga
    15421 ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac
    15481 tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc
    15541 agaatgaaga gtgatttatg tgatatgaat cacttttggt gggtaaattt atgcaacgca
    15601 tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt
    15661 tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt
    15721 catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac
    15781 ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac
    15841 cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc
    15901 ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc
    15961 accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc
    16021 ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa
    16081 ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatctatt
    16141 ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa
    16201 cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg
    16261 caggcgatgg gcgtaatgaa ctatgaaatg gaatctgcaa ctctgctgac catgtgtgca
    16321 agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag
    16381 atcccgaatg ctgagacgat gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa
    16441 gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt
    16501 tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca
    16561 attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg
    16621 tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac
    16681 atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg
    16741 cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgtgaa ttgctcaata
    16801 acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa
    16861 ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca
    16921 gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca
    16981 atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg
    17041 aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac
    17101 gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg
    17161 aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg
    17221 aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa
    17281 cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc
    17341 tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct
    17401 attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg
    17461 tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa
    17521 ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc
    17581 gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta
    17641 cgctgctggt ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgta
    17701 acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg
    17761 atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa
    17821 tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt
    17881 taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccagg
    17941 atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg
    18001 atgatgaata taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat
    18061 ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt
    18121 gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa
    18181 caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg
    18241 aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc
    18301 ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg
    18361 aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc
    18421 atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat
    18481 gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt
    18541 tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg
    18601 ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg
    18661 agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg
    18721 aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat
    18781 accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg
    18841 gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt
    18901 taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc
    18961 cgcgctgaaa acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg
    19021 cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg
    19081 ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga
    19141 tcgccagcag cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca
    19201 ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct
    19261 ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc
    19321 aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga
    19381 agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc
    19441 cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg
    19501 ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat
    19561 gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc
    19621 tggatgccaa atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa
    19681 gaactggggc cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt
    19741 ccaccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc
    19801 aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt
    19861 gacgattttg aaatcaagcc gctggcttct gcttctatcg cccaggttca taccgcgcga
    19921 ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtc
    19981 attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg
    20041 gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat
    20101 gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgcaa ttttgaagac
    20161 agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg
    20221 atggagcgca tttacggcat tccggtgtct gatgttgcgg cgctggagaa aaacggcact
    20281 aacatgaaat tgctggcgga acgcggcgtg caggtgttct tcactcaggt ctttcgcgac
    20341 agctttttcc atgccgatat gcaccctggc aacatcttcg taagctatga acacccggaa
    20401 aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa
    20461 cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag
    20521 ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc
    20581 attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat
    20641 gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg
    20701 gtgttactcc agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag
    20761 ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt
    20821 attcctgccc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca
    20881 gaactgcctg aactggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt
    20941 gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt
    21001 ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgag
    21061 tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg
    21121 cgcaaaacac gctgattttt tcatcgctca aggcgggccg tgtaacgtat aatgcggctt
    21181 tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta
    21241 ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt
    21301 tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag
    21361 caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg
    21421 gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg
    21481 taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc
    21541 gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg
    21601 ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagtttca ggacagtctg
    21661 aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg aactgaaagc gtcgatggat
    21721 gaactacgcc aggccgcgga gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag
    21781 gcgagcgatg aagcgcacac catccataac ccggtggtga aagataatga agctgcgcat
    21841 gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt cgccggaaca gaagccagaa
    21901 accacgccag agccggtggt aaaacctgct gcggacgctg aaccgaaaac cgctgcccct
    21961 tccccttcgt cgagtgataa accgtaaaca tgtctgtaga agatactcaa ccgcttatca
    22021 cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat
    22081 tcctgtgtct ggtctatttc gccaatgaca tctatcacct ggtatccgcg ccattgatca
    22141 agcagttgcc gcaaggttca acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc
    22201 cgatcaagct gacctttatg gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg
    22261 tgtgggcatt tatcgcccca gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc
    22321 tggtttccag ctctctgctg ttttatatcg gcatggcatt cgcctacttt gtggtctttc
    22381 cgctggcatt tggcttcctt gccaataccg cgccggaagg ggtgcaggta tccaccgaca
    22441 tcgccagcta tttaagcttc gttatggcgc tgtttatggc gtttggtgtc tcctttgaag
    22501 tgccggtggc aattgtgctg ctgtgctgga tggggattac ctcgccagaa gacttacgca
    22561 aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg
    22621 atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct
    22681 tcttctcacg cttttacgtt ggtaaagggc gaaatcggga agaggaaaac gacgctgaag
    22741 cagaaagcga aaaaactgaa gaataaattc aaccgcccgt cagggcggtt gtcatatgga
    22801 gtacaggatg tttgatatcg gcgttaattt gaccagttcg caatttgcga aagaccgtga
    22861 tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg ctactcatca ccggcactaa
    22921 cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac
    22981 ggcgggcgta catcctcacg acagcagcca gtggcaagct gcgactgaag aagcgattat
    23041 tgagctggcc gcgcagccag aagtggtggc gattggtgaa tgtggtctcg actttaaccg
    23101 caacttttcg acgccggaag agcaggaacg cgcttttgtt gctcagctac gcattgccgc
    23161 agaattaaac atgccggtat ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt
    23221 gctggagccg tggctggata aactgcctgg tgcggttctt cattgcttta ccggcacacg
    23281 cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc ggcattaccg gttgggtttg
    23341 cgatgaacga cgcgggctgg agctgcggga attgttgccg ttgattccgg cggagaaatt
    23401 gctgatcgaa actgatgcgc cgtatctgct cccgcgcgat ctcacgccaa agccatcatc
    23461 ccggcgcaac gagccagccc atctgcccca tattttgcaa cgtattgcgc actggcgtgg
    23521 agaagatgcc gcatggctgg ctgccaccac ggatgccaat gtcaaaacac tgtttgggat
    23581 tgcgttttag agtttgcgga actcggtgtt cttcacactg tgcttaatct ctttattaat
    23641 aagattaagc aatagcatgg agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa
    23701 gccttcgaac gcgccttcgg taataatcac cttatcaccc ggataagggg gtgccggatc
    23761 gacaatgtct ttcggtttat ataccgatag ctgatgaatc accgccgatg ggactatcgc
    23821 tggcgacgcg ccaaagcgca caaagtggct gacgccacgc gtagcgctga tggtcgtggt
    23881 atgaatcact tctggatcaa attccacaaa caggtagtta gggaataacg gttcactgac
    23941 tgcggtacgt ttaccccgta cgattttttc cagggtgatc atcggagcga ggcaattaac
    24001 cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa
    24061 ataccaggat tgcataatga ctcttatccg tttaatcggg gcgcaaggat agcaaaagct
    24121 ttacgctaag ttaattatat tccccggttt gcgttatacc gtcagagttc acgctaattt
    24181 aacaaattta cagcattgca aagatgaacg ccgtataatg ggcgcagatt aagaggctac
    24241 aatggacgcc atgaaatata acgatttacg cgacttcctg acgctgcttg aacagcaggg
    24301 tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg gaaatcactg aaattgctga
    24361 ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa aaccctaaag gctactcaat
    24421 gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga
    24481 agatgtttcg gcgctgcgtg aagttggtaa attattggcg tttctgaaag agccggagcc
    24541 gccaaaaggt ttccgcgacc tgtttgataa actgccgcag tttaagcagg tgttaaacat
    24601 gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt
    24661 cgatctcaat cgcattccca ttatgacctg ctggccggaa gatgccgcgc cgctgattac
    24721 ctgggggctg accgtaacgc gcggcccgca taaagagcgg cagaatctgg gcatttatcg
    24781 ccagcagctg attggtaaaa acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc
    24841 gctggattat caggagtggt gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc
    24901 gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc cccgttccgg atacgctttc
    24961 agagtatgcg tttgccggat tgctacgcgg caccaaaacc gaagtagtaa agtgtatttc
    25021 caatgacctc gaagtgccag ccagtgccga aatcgtcctg gaagggtata tcgatcctgg
    25081 tgagatggcg ccggaagggc cgtatggtga ccacacaggt tactataatg aagtcgatag
    25141 tttcccggtg tttaccgtga cgcatattac ccagcgtgaa gatgcgattt accattccac
    25201 ctataccggg cgtccgccag atgagcccgc ggtactgggc gtggcgttga acgaagtgtt
    25261 tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat ttttacctgc cgccggaagg
    25321 ctgttcttat cgtctggcgg tagtgacgat caaaaaacag tacgccggac acgcgaagcg
    25381 cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg tacactaaat ttgtgatcgt
    25441 ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg atttgggcga ttaccacccg
    25501 tatggacccg gcgcgggata ctgttctggt agaaaatacg cctattgatt atctggattt
    25561 tgcctcgcct gtctccgggc tgggttcaaa aatggggctg gatgccacga ataaatggcc
    25621 gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca
    25681 tattgacgcc atctgggatg aactggctat ttttaacaac ggtaaaagcg cctgatgcgc
    25741 gtttgttttg ccctatttat cgatccgaca gagaaagcgc atgacaacct taagctgtaa
    25801 agtgacctcg gtagaagcta tcacggatac cgtatatcgt gtccgcatcg tgccagacgc
    25861 ggccttttct tttcgtgctg gtcagtattt gatggtagtg atggatgagc gcgacaaacg
    25921 tccgttctca atggcctcga cgccggatga aaaagggttt atcgagctgc atattggcgc
    25981 ttctgaaatc aacctttacg cgaaagcagt catggaccgc atcctcaaag atcatcaaat
    26041 cgtggtcgac attccccacg gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat
    26101 tttgattgcg ggcggcaccg ggttctctta tgcccgctcg attttgctga cagcgttggc
    26161 gcgtaaccca aaccgtgata tcaccattta ctggggcggg cgtgaagagc agcatctgta
    26221 tgatctctgc gagcttgagg cgctttcgtt aaagcaccct ggtctgcaag tggtgccggt
    26281 ggttgaacaa ccggaagcag actggcgtgg gcgtactggt accgtgttaa cggcggtatt
    26341 gcaggatcat ggtacgctgg cagagcatga tatctatatt gccggacgtt ttgagatggc
    26401 gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg
    26461 cgatgcgttt gcatttatct gagatataaa aaaacccgcc cctgacaggc gggaagaacg
    26521 gcaactaaaa ctttattcag tggcatttag atctatgtcg catccggcaa ttggtgcacg
    26581 atgcctgatg cgacgctggc gcgtcttatc aggcctacat tggtgccgga tcggtagacc
    26641 ggataaggcg ttcacgccgc atccggcaag tggttaaacc cgctcaaaca ccgtcgcaat
    26701 accctgaccc ataccgatac acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat
    26761 cagattcagc agcgtggtgc tgatacgcgc accggaacaa cccagcggat gacccagcgc
    26821 gatcgcacca ccattgaggt tgatcttctc gtcaatctgc tccattagcc cgagatcttt
    26881 aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct
    26941 ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat
    27001 aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc
    27061 taattcatgg gcgcggcttt cactcatcac cagcatggca gctgcgccat cggaaagtgc
    27121 agaagatgtg cccgccgtta ccgtaccgtt tactggatca aacgccggac gcagcgtggc
    27181 gagggcttcc acggtggttt ccgggcgaat cacttcgtcg taattaaact gcttcaggac
    27241 gccgtcggca tcgtgaccac cagttgggat gatttcattt ttaaatgcgg ccgactgcgt
    27301 ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat
    27361 accgtgcata cgcgccagca tttctgccgt taagcccatc atgcccgccg ctttggcgac
    27421 attgcggctc aggccggggt gaaaatcgac gccgtgactc atcggcacat ggcccatatg
    27481 ctccacgccg ccaaccagac atgcctgcgc atcgccagtc atgatcattc gtgctgcgtc
    27541 atgcagtgcc tgcatggatg aaccacacaa gcgattgacg gtaaccgccg ggacagagtg
    27601 cggcacttct gccagcagcg ccgcgttacg ggcgatgtta aaaccctgct ccagcgtctg
    27661 ttgcacacaa ctccagtaaa tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc
    27721 cagcaggcta cgcattaaat gagcggagag atcttccgca cgcacgttac gaaaagcacc
    27781 gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca atgacaacct gttccattgt
    27841 gactccttaa gccgttttca ggtcgccaac cggacgggct ggctcaaccg gaggatagta
    27901 cggttcgtta tgacgcgctt tattacgcag accttccggc acttcataca gcgggccgag
    27961 gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg
    28021 gaacgcgccg ccgtggaacg gagggaagcc caggccgtag accagcgcca tatccgcttc
    28081 cgccggagtg gcgataatgc cctcttccag acagcgcacc acttcgttga ccatcgggat
    28141 catcatgcgg gcgataatct cttcttcgct gaaatcgcgc ttcggttggc tcacttctgc
    28201 cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt
    28261 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    28321 gatggcatcg cggtaatctt tctgcatccg ctgcgggaag cctgctgcca tgacagcctg
    28381 agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga tatgccgggc ccatcggcca
    28441 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag
    28501 ctggctgaaa ccggcgaaat acgggaacag cacgcggtta acaaagaagc cggggcagtc
    28561 gttaaccaca atcggcgtct tgcccatctt gctcgcccag gcgacaactt tcgcgatggt
    28621 ttcgtcggag cttttctcgc cgcgaataat ttctaccaac ggcattcggt ggaccgggtt
    28681 aaagaagtgc atcccgcaga agttttccgg gcgttccagc gcgttggcca gttcgctgat
    28741 aggaatggtt gaagtgttag acgccagcac ggtatcctgg cgtacttttt gttcggtttc
    28801 tgccagtacg gcttttttca ctttcgggtt ttcaacaacc gcttctacca caatatccac
    28861 gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg gagatcacgc cagccagttt
    28921 cagaccatcg atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat
    28981 gccgagggtt aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc
    29041 ggactggtaa gcgataccgc cacccatgat gcctgcgccc agcacagcag cctgtttcgg
    29101 ggtttcaacg tctttggtga gtttcttcgc tttgcctttt acatattgat cgttaaggaa
    29161 aatgccgacc agtgcgcgag cttcgttggt atgcgccagc gggacaaaac ttttgttttc
    29221 cagatttaag gcttcttcac gaccaaaacg ggccgcagct tcaatggttt ttactgcggt
    29281 gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc attcctttag cgatggtgaa
    29341 gctcatggtg gcttcaatct tgctcagttt tagcggttcc agcttcggct gacgtttcgc
    29401 tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc gccatcgccc cttcaatcag
    29461 tttttctgct ttgactacgc catccaccag accgattttc agcgcctgat ccgcgccgac
    29521 atctttaccg gcggcaatga tttccagtgc actgtcagcg cccagcatac gtggcatacg
    29581 tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt tccggcagac cgatgcgcag
    29641 atccggcgtc gccagacgat aatcggtcgc cagcacgcat tcgcagccac cacccagcgc
    29701 atagccgttg acggcagcaa tggtcggcac cggcagatct tccaggcgat taaacacgct
    29761 attggcaaag tgcagccact gacttaactg ttcttcagga acgaggaaca gggacaaaaa
    29821 ttcggtgata tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt
    29881 tagatctgat tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt
    29941 gtcgagttta ttaactgaac ctggggcatc aaataccagt tcggcaatgc catcttccag
    30001 ccagtcaagg tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt
    30061 gatctggtcg taccagatgc atcggagtgt gtgttttgtg ttaaaaatat gcaaataaaa
    30121 gattaaagaa atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt
    30181 gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc
    30241 tcgctctata aaaatcatat agctacctta caagaacgga ctcgcgatgc gctggcgcgc
    30301 ttcaagctgg atgcattact tattcactcc ggcgagctgt tcaacgtttt tctcgacgat
    30361 catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg
    30421 ccaaactgct ggttgctggt ggatggcgtg aacaagccga aactgtggtt ctatctgccg
    30481 gttgattact ggcacaacgt cgaaccgctg ccgacctcct tctggactga agatgtggaa
    30541 gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc tgcctgctgc gcgcggcaat
    30601 atcggttata tcggtccggt gccggaacgt gcgctgcaac tgggtattga ggccagcaac
    30661 atcaacccga aaggggtgat cgactacctg cattactacc gctccttcaa aaccgagtac
    30721 gaactggcct gtatgcgtga agcgcagaaa atggcggtca atggtcatcg tgcggcagaa
    30781 gaagcgttcc gttctggcat gagcgagttc gatatcaata ttgcctatct gaccgcgacc
    30841 ggtcatcgtg ataccgacgt accttacagc aacattgtgg cgcttaacga acacgctgcg
    30901 gtgttgcatt acaccaaact ggaccaccag gcaccggaag agatgcgcag cttcctgctg
    30961 gatgccgggg ccgaatataa cggctatgcg gctgacctga cccgtacctg gtcggcaaaa
    31021 agcgacaacg actacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc
    31081 gccaccatga aagcaggcgt cagctatgtg gattaccaca tccagttcca tcagcgcatc
    31141 gccaaactgc tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa
    31201 aacgatctca ccggaccgtt tatgccgcac ggtattggcc atccgctggg cctgcaggtg
    31261 catgacgtcg ccggttttat gcaggatgat agcggtacac acctcgcggc accggcaaaa
    31321 tatccgtacc tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg
    31381 ggtatctact tcattgaatc gcttctggca ccgtggcgtg aagggcagtt cagcaagcac
    31441 ttcaactggc agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac
    31501 gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg
    31561 aaagctggtt aattcctgcg gcaccggtca cggtcgttga agagatcaaa aagagccgct
    31621 tcataacgat gttggcgcat accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg
    31681 tgcgggcaga acaccccgat gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg
    31741 atgattctca acagctgggt ttctctgacg acggggagcc ggcgggaacg gcaggtaaac
    31801 cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat taccgctgtg gtagtgcgct
    31861 actacggcgg catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga
    31921 atcaggcgct gcgccagcta acgacccaac gcaagacgcc attaaccgaa tatactttgc
    31981 aatgtgaata tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa
    32041 ttatcaacag tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag
    32101 tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga
    32161 ttgaagaata atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg
    32221 cgccattacc cgaatcgttg gactactggt catcttattt tcagggacca tgattatccc
    32281 tgggctggta gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt
    32341 tgtcgccctc gccattggct ctatgctgtg gtggccgaac cgcaaagaga agggcgaact
    32401 gaaatcccgt gaggggtttc tgatagtggt gctgttttgg accgtgctgg gtagtgtcgg
    32461 tgcgctccct tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga
    32521 atctttctct ggcctgacca ccaccggggc cactacgctg gtggggctgg attcgctccc
    32581 tcatgccatc ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt
    32641 gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga
    32701 aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac
    32761 cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg
    32821 aatggatgcc attgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc
    32881 gacacatgat gccagtatcg gttatttcga cagtccgact attaacacta tcattgctat
    32941 cttcctgctg atctccggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag
    33001 tctgaaggtt tattggcgcg atccggaatt tcgcatgttt atcggcgtac agtttacgct
    33061 ggtggttatt tgtacactcg tactgtggtt tcataatgtc tacagttcgg cgctgatgac
    33121 aattaaccag gcgtttttcc aggtggtatc gatggcgaca accgccgggt ttacgactga
    33181 cagcattgcc cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg
    33241 cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa
    33301 gcaggggaac cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct
    33361 ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt tggggatttt tctccgccta
    33421 tgcattggtg tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgatttttc
    33481 tgcctttgcc tcagttgttg cgacattgaa taacctgggg ccagggcttg gcgtggttgc
    33541 tgataacttt accagtatga acccggtggc taaatggatc ctgattgcca acatgctgtt
    33601 tggtcgtctc gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg
    33661 atggagtaat acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag
    33721 attgcctcct acctggcttc ggaactgaaa gaactgggga tccagacgga tgtcgccaat
    33781 gtgcaccgca ttgaagaacc acaatgggaa aactatgacc gtgtggtcat tggtgcttct
    33841 attcgctatg gtcactacca ctctgcgttc caggaatttg tcaaaaaaca tgcgacgcga
    33901 ctgaattcga tgccgagcgc cttttattcc gtaaacctgg tggcgcgcaa accggagaag
    33961 cgtactccac agaccaacag ctacgcgcgg aagtttctga tgaactcgca atggcgtccc
    34021 gatcgctgcg cggtcattgc cggggcgctg cgttacccac gttatcgctg gtacgaccgt
    34081 tttatgctca agctgattat gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt
    34141 gtctataccg attgggagca ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac
    34201 aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc ggtcagaaaa ttattttaaa
    34261 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
    34321 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccg
//
LOCUS       DADLSE010000030        34215 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLSE010000030 DADLSE010000000
VERSION     DADLSE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.030"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..3148)
                     /gene="recB"
                     /locus_tag="JN692_001427"
     CDS             complement(<1..3148)
                     /gene="recB"
                     /locus_tag="JN692_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7263773.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAG"
     gene            complement(3141..6029)
                     /gene="ptrA"
                     /locus_tag="JN692_001428"
     CDS             complement(3141..6029)
                     /gene="ptrA"
                     /locus_tag="JN692_001428"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7263774.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKDGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AIITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(6205..9573)
                     /gene="recC"
                     /locus_tag="JN692_001429"
     CDS             complement(6205..9573)
                     /gene="recC"
                     /locus_tag="JN692_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7263775.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKTLVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(9586..9909)
                     /locus_tag="JN692_001430"
     CDS             complement(9586..9909)
                     /locus_tag="JN692_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7263776.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(9894..10301)
                     /locus_tag="JN692_001431"
     CDS             complement(9894..10301)
                     /locus_tag="JN692_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7263777.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHCWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(10298..10861)
                     /locus_tag="JN692_001432"
     CDS             complement(10298..10861)
                     /locus_tag="JN692_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7263778.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(10852..11322)
                     /locus_tag="JN692_001433"
     CDS             complement(10852..11322)
                     /locus_tag="JN692_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7263779.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLYQQGETEGCL"
     gene            complement(11506..12300)
                     /gene="thyA"
                     /locus_tag="JN692_001434"
     CDS             complement(11506..12300)
                     /gene="thyA"
                     /locus_tag="JN692_001434"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7263780.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(12307..13182)
                     /gene="lgt"
                     /locus_tag="JN692_001435"
     CDS             complement(12307..13182)
                     /gene="lgt"
                     /locus_tag="JN692_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7263781.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(13333..15579)
                     /gene="ptsP"
                     /locus_tag="JN692_001436"
     CDS             complement(13333..15579)
                     /gene="ptsP"
                     /locus_tag="JN692_001436"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7263782.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(15592..16122)
                     /gene="rppH"
                     /locus_tag="JN692_001437"
     CDS             complement(15592..16122)
                     /gene="rppH"
                     /locus_tag="JN692_001437"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7263783.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            16807..17496
                     /gene="mutH"
                     /locus_tag="JN692_001438"
     CDS             16807..17496
                     /gene="mutH"
                     /locus_tag="JN692_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7263784.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            17565..18278
                     /locus_tag="JN692_001439"
     CDS             17565..18278
                     /locus_tag="JN692_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7263785.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            18416..18634
                     /gene="ygdR"
                     /locus_tag="JN692_001440"
     CDS             18416..18634
                     /gene="ygdR"
                     /locus_tag="JN692_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7263786.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            18742..19782
                     /locus_tag="JN692_001441"
     CDS             18742..19782
                     /locus_tag="JN692_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7263787.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(19815..21008)
                     /gene="lplT"
                     /locus_tag="JN692_001442"
     CDS             complement(19815..21008)
                     /gene="lplT"
                     /locus_tag="JN692_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7263788.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(21001..23160)
                     /gene="aas"
                     /locus_tag="JN692_001443"
     CDS             complement(21001..23160)
                     /gene="aas"
                     /locus_tag="JN692_001443"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7263789.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSCQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            23745..24776
                     /gene="galR"
                     /locus_tag="JN692_001444"
     CDS             23745..24776
                     /gene="galR"
                     /locus_tag="JN692_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7263790.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(24783..26045)
                     /gene="lysA"
                     /locus_tag="JN692_001445"
     CDS             complement(24783..26045)
                     /gene="lysA"
                     /locus_tag="JN692_001445"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7263791.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            26167..27102
                     /gene="lysR"
                     /locus_tag="JN692_001446"
     CDS             26167..27102
                     /gene="lysR"
                     /locus_tag="JN692_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7263792.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(27089..27781)
                     /gene="ygeA"
                     /locus_tag="JN692_001447"
     CDS             complement(27089..27781)
                     /gene="ygeA"
                     /locus_tag="JN692_001447"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7263793.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(27910..29328)
                     /locus_tag="JN692_001448"
     CDS             complement(27910..29328)
                     /locus_tag="JN692_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7263794.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(29620..30381)
                     /gene="kduD"
                     /locus_tag="JN692_001449"
     CDS             complement(29620..30381)
                     /gene="kduD"
                     /locus_tag="JN692_001449"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7263795.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(30411..31247)
                     /gene="kduI"
                     /locus_tag="JN692_001450"
     CDS             complement(30411..31247)
                     /gene="kduI"
                     /locus_tag="JN692_001450"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7263796.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(31534..32708)
                     /locus_tag="JN692_001451"
                     /pseudo
     CDS             complement(31534..32708)
                     /locus_tag="JN692_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
     gene            32963..34192
                     /locus_tag="JN692_001452"
     CDS             32963..34192
                     /locus_tag="JN692_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7263797.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLCIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPSSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNHA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         8484 a         9279 c         8315 g         8137 t
ORIGIN      
        1 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
       61 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
      121 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
      181 acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
      241 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
      301 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
      361 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
      421 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agtgtcctcg
      481 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
      541 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
      601 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
      661 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
      721 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
      781 cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac gagtggcgat
      841 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
      901 gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt ttatcacttt
      961 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
     1021 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc agttcgctga
     1081 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
     1141 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
     1201 gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca ttattcagcg
     1261 tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt
     1321 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
     1381 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
     1441 catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg tccgaagcac
     1501 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
     1561 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
     1621 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaata
     1681 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
     1741 acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attccaggtg
     1801 cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc gccttcatat
     1861 aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca
     1921 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
     1981 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
     2041 acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
     2101 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
     2161 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
     2221 acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag aaacgctggg
     2281 agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct gcccaggcgc
     2341 tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
     2401 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat
     2461 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
     2521 ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
     2581 gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc
     2641 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
     2701 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaagccgt
     2761 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
     2821 cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt
     2881 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg
     2941 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc gggcggggaa
     3001 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
     3061 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
     3121 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
     3181 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
     3241 ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga
     3301 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
     3361 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
     3421 ccgagcgttt gcggtgcctg cagcatctgg gtaattatcg cctgctggat ttgcgcaaac
     3481 tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
     3541 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
     3601 cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc ggtacgcaac
     3661 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg
     3721 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
     3781 tctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt tcgacaccac
     3841 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc
     3901 tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
     3961 taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc atctcgcgag
     4021 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc
     4081 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgctcttcc
     4141 gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg
     4201 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
     4261 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
     4321 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
     4381 acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc
     4441 gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat cagcgagaaa
     4501 tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc
     4561 tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaag
     4621 taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
     4681 gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc aatattgact
     4741 gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa
     4801 tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt cgccagttca
     4861 tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct aaaaattgcc
     4921 gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga gatcgctaat
     4981 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
     5041 tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag ataggtaatc
     5101 aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
     5161 aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
     5221 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
     5281 tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac cgccttcatc
     5341 aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
     5401 ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
     5461 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
     5521 aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct
     5581 gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga
     5641 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
     5701 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
     5761 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcactacc
     5821 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
     5881 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
     5941 atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac aaacaacaat
     6001 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg
     6061 gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
     6121 cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag taagcggata
     6181 gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg gtaacaggaa
     6241 acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagccg
     6301 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
     6361 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
     6421 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
     6481 cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
     6541 aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt cgccattacc
     6601 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
     6661 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
     6721 ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat
     6781 gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa
     6841 agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc
     6901 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gtccttccag
     6961 aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca cctgcaaacg
     7021 catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
     7081 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
     7141 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
     7201 cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
     7261 ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat
     7321 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
     7381 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc
     7441 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
     7501 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
     7561 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
     7621 gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg acagcggcac
     7681 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
     7741 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
     7801 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
     7861 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
     7921 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
     7981 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg
     8041 aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata
     8101 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagcagcgc
     8161 cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga taaacgcttc
     8221 cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca ggtaacgatc
     8281 cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga tatcagccac
     8341 catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg ccagcaagcg
     8401 atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg tgatactgct
     8461 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
     8521 cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
     8581 tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag aaaggagata
     8641 aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga catcctgttc
     8701 accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg
     8761 atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag caggatcttt
     8821 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
     8881 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
     8941 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
     9001 ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat attccaccag
     9061 cgtcttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
     9121 tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa acaggtccgc
     9181 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg
     9241 caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca gtttccagct
     9301 catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc ggacgaacat
     9361 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
     9421 cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg
     9481 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
     9541 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
     9601 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
     9661 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga
     9721 aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct
     9781 gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga caatcatcac
     9841 catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat tcttcaggga
     9901 agctgacata atgccccctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
     9961 acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    10021 atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc ggttccagcg
    10081 tactgcgagc actgaactgc tggctgcgtc tgccagcagt gcatttttcc ccacgccagc
    10141 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca
    10201 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac cagcagcatc
    10261 aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat cctgtcacgc
    10321 tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    10381 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    10441 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    10501 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt
    10561 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc
    10621 cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat
    10681 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc ttacgagtgc
    10741 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    10801 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    10861 tccttctgtt tctccttgct gataaagccg gagtcttccc cacggagaaa ccaccagcca
    10921 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    10981 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    11041 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    11101 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    11161 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc gttgcgactg
    11221 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc
    11281 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    11341 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    11401 ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa
    11461 aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga tagccaccgg
    11521 cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga aacggtagtc
    11581 gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg gttcgcggct
    11641 taattgcaga tgagtttgat ccatatggtt gctgtacagg tgcgtatcgc caccggtcca
    11701 gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca gtaacgcgta
    11761 gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat aaagctggca
    11821 agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg ccagcgccat
    11881 tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat ccgggtcgtt
    11941 tttcagctgg ttcagtaccg tagtaatctg gtcaatatga cgaccatctg gcgttggcca
    12001 ggcgcgccac tgtttaccat acactggccc gaggtcgcca ttttcatcgg cccattcgtc
    12061 ccagatggtg acattgtttt cgtgtagata agcaatgtta gtatcgccct gcaggaacca
    12121 cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg gttgtcacca gcgggaatcc
    12181 atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg ttccggtacg
    12241 gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat actgtttcat
    12301 ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca tcatgatcac
    12361 acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact gcacccaggc
    12421 accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa acgcgccgta
    12481 gccaatcagg aacaagcctg agacagctcc cattgggcgc ggtttacgaa tatacaggtt
    12541 gaggataata aacagcacca caccttccag cagcagttcg taaagctgtg atgggtggcg
    12601 cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt gcagcagcaa
    12661 aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg ggtcaacgcg
    12721 gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac caaacggaat
    12781 gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc gggcgaagat
    12841 aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc cgtcccagac
    12901 acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca gaacataacc
    12961 aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt caacttcatt
    13021 tttggtccag ccactgcccg gacgattcgc ccgacgtgtt gccagccaca ttgcaaaaat
    13081 gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat
    13141 gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc acaagttctt
    13201 gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa
    13261 aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta aaagatatgt
    13321 atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac gctccataaa
    13381 ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct tccagactac gctgcgcaag
    13441 attttctgct tcggcaaaat caatgcgccg cagcaggtat tttacccgcg ctacagaacg
    13501 tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc acatgggatc
    13561 gcccgccatt tcaccgcaca aacggagatc gataccatgt atttccgctt cccgggcgat
    13621 catcgccaga gctcgtaaca ttgcaggatg aagactgtcg taaatgttcg ccacccgggt
    13681 attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa cagagatgaa
    13741 atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt ccagcatgat
    13801 gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac gtccggcacg
    13861 ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg gtgaccatcg gcaacagaat
    13921 attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga tcaagaagat
    13981 ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat tctcttcgct
    14041 gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca aggtgacggg
    14101 tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt cttccgacgg
    14161 gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc catcaatacg
    14221 gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga cttttatacg
    14281 ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac ggctaagctc
    14341 aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg gatcgaccag
    14401 caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg aaggctgaat
    14461 atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag catgggagtt
    14521 ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct cagcaagagt
    14581 tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg
    14641 attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat cgccagcccg
    14701 ctctttgaga tagttatcgc ttagcgcggc aaactgttca gcaaattttt caatgaccgt
    14761 ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag
    14821 ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc
    14881 tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc
    14941 ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca cctgttccat
    15001 taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa
    15061 tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg actgagaaag
    15121 aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt cgtcatactg
    15181 gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt gaataattgg
    15241 tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt tgaagctggg
    15301 gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca ggccgacgat
    15361 cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc
    15421 catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga cctcggtatc
    15481 catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc gtggtgcgct
    15541 ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga cttaacctct
    15601 tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca gtgacatcac
    15661 cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg acaccacctg
    15721 tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg tactgctggt
    15781 ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt tttgtttttg
    15841 gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa
    15901 ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta cttcttcaaa
    15961 caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc cttgcggaaa
    16021 ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc gattacaaat
    16081 cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac ctcaaaataa
    16141 agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac tctctgcagg
    16201 gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta taaacagagg
    16261 aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga agaactacgg
    16321 aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat tttaaaatat
    16381 tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc
    16441 aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat ttatccacag
    16501 aatgagccac taagttaagc actgaaccac taaaaactgg agtttcgtcg cacgtcaagg
    16561 ctgtaaatga aaacagtagt ggaggttttt cacagttatc ccagctttct gtggataaca
    16621 tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg cagtgttgcg
    16681 caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac aacacatgtg
    16741 gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta accaaggtat
    16801 catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga acagttgtta
    16861 gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact tgccgggctg
    16921 gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact ggagatctgg
    16981 ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg cgtggaactt
    17041 aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc
    17101 ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa
    17161 cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta
    17221 ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg cgaagactgg
    17281 gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc tcgtcacggg
    17341 gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga agccattggt
    17401 gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa gaatttcacc
    17461 agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc tgccgctttc
    17521 cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga
    17581 taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag atcgttcttg
    17641 ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg
    17701 ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg ctgctggcat
    17761 caatctcttg ggttacgcgc ctgacaaatc cgctttttac aatattcagt caggaaattt
    17821 ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg
    17881 aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc
    17941 tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac tcggtgatta
    18001 ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg attgccgtag
    18061 gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat ccttcggtaa
    18121 aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg
    18181 acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa
    18241 gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc tctcctgcag
    18301 gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt caagatatat
    18361 aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata accggatgaa
    18421 aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccag
    18481 tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat
    18541 tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa
    18601 ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat ccggctggcc
    18661 ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt
    18721 gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc gctggaagtc
    18781 agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac
    18841 gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac
    18901 ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg
    18961 gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc
    19021 aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg
    19081 ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca ggtgcactgg
    19141 ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga ttctgcgcct
    19201 gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc gggaaaaatt
    19261 cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg
    19321 gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct gttaaaccgc
    19381 agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat
    19441 tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa acccgctggc
    19501 gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc
    19561 gtcgcggcgt atgttgatat cgccagacgt catggccttg atcctgctca gatggcgctc
    19621 gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac cacgatggat
    19681 cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt attagctgaa
    19741 attgaagcgg tacatcaggt ttatacttat cctgcaccat aaagattaac gccggttaaa
    19801 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    19861 aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc
    19921 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    19981 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    20041 acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg cagcagctcg
    20101 tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
    20161 gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac gataccaatc
    20221 gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag cgccaccggt
    20281 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    20341 accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt caggaaactg
    20401 cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg
    20461 tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgaccagg
    20521 acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc cgctatggta
    20581 gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa ttcgccgaga
    20641 ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga
    20701 aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac
    20761 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgccaaaaga
    20821 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    20881 gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc agagagaaac
    20941 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    21001 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt
    21061 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta ctgcaagctc
    21121 cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt
    21181 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    21241 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc
    21301 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa
    21361 acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc
    21421 ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg
    21481 ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgtgcatc
    21541 cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    21601 aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc caaatttatc
    21661 ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca catagcgtag
    21721 acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtacc
    21781 gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt aatgcagcgg
    21841 gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag
    21901 cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg tgaagtcggc
    21961 aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt
    22021 tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt
    22081 tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt
    22141 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    22201 atgccagagt ttgcctttat cgagaaactg gcaggaagtg aagatggttt tgatttcagc
    22261 cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca ttgcgggaat
    22321 gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    22381 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    22441 tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt
    22501 cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt cacgcgggcg
    22561 caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    22621 gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa tatgcagagt
    22681 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    22741 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc
    22801 gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac
    22861 cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg
    22921 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    22981 tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc
    23041 aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc
    23101 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    23161 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    23221 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    23281 aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgggatctt
    23341 gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat
    23401 gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc
    23461 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    23521 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    23581 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc ttttttgtct tacttatttg
    23641 cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc
    23701 gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    23761 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    23821 ccaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    23881 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    23941 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    24001 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    24061 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    24121 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    24181 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    24241 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    24301 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    24361 gtattccggc caatgatcgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    24421 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    24481 attcaatggc ggcgggcgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    24541 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt ccgcgcctga
    24601 ccaccgtgcg ttacccaatc gtaacgatgg cgacccaggc tgccgaactg gctttggcac
    24661 tggcggataa tcgccctctc ccggaaatta ctaatgtatt tagtccgacg ctggtacgtc
    24721 gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    24781 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    24841 gcctgaccat tatcaaacag aacttctggt aacagcggac ggctattgta gttggatgac
    24901 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacctgc cttcacttcc
    24961 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    25021 cataacggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    25081 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    25141 aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga
    25201 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    25261 gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc ataataatgt
    25321 tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc gcccgcagaa
    25381 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgc
    25441 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac tagctgcaga
    25501 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    25561 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    25621 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    25681 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    25741 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    25801 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    25861 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacttcaaa ctgtttcagc
    25921 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    25981 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    26041 ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata aaaagaataa
    26101 aaaatatcga tttatgtcga gtctatgcaa aaatgatatg gattaccgga ttgcgagaga
    26161 gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt aatgaccgcc
    26221 ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt cagccgcgaa
    26281 ctggcgcgct ttgagaaggt gatcgggctg aaattgtttg agcgcgtacg tggacgatta
    26341 catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg gtacggactg
    26401 gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga actgtctatt
    26461 gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc ctttctggca
    26521 cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact tgaagagtgg
    26581 ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc tgcgggaaca
    26641 gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg
    26701 gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaattacat cagcctttcc
    26761 cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca ggttaaacga
    26821 cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg ggcgggggta
    26881 ggtgtttcgg tggttaaccc gctcaccgca ctagattatg cggcaagcgg tttagtggtg
    26941 cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct gcaccgcccg
    27001 tcatcagcgc tggttcaggc gtttagtggg catttacaag cggggttacc gaaactagtc
    27061 acttctcttg acgctatttt gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg
    27121 catggatcgc cgcggtatca aacacaggca aaacactgcg ctcttctggc accagtaaac
    27181 caatttctgt gcagccaaaa atgacgccct gtgcgccctg ttctgcaagg cgagcaatca
    27241 cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt tcttcaaaaa
    27301 taatctgatt aattttcgcc cgttcatccg cttcaggaat aagacaattg atggaaaatt
    27361 gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta cccagcagcg
    27421 ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca atgtgtaaga
    27481 aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta ttggtacata
    27541 acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc agaatgtccc
    27601 cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa tcgacgctat
    27661 gtagcagcat ttgcgcagag tgaagcccac caagccgctg tttaatgcct tcatttatca
    27721 gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca attgttttca
    27781 ttcctctctc ctgatgtgtg ttaccgcaac aaggctgaat aaggaacgag acaaacgcct
    27841 caacggccag gtgcccaatc tctattaacg aaaaaagggc cggatgtaca gcacatccgg
    27901 cccgtgaaat cagacgccga tatttctcaa cttctcgcct gccatcagtt tgcgttcgat
    27961 atgttccagc gtgacatttt tggtttccgg aatgagccag aaagtaatgc ccacaaacgc
    28021 aatgttcagc gcagtgtaga gccagaacgt accggcagcg ccaatgctat caagcagtgt
    28081 caggaaggtc gcgccgataa tcatattcga cacccagttt gtcgtcgtcg aacaggtaat
    28141 accgaaatcg cggcatttca gcggctgaat ttcagagcac aggatccaca ccactggcgc
    28201 ggcgctcatc gcataaccgg caatacacat catcgtcatg ccaacagaga gccaagacaa
    28261 gccactggaa gccgtaccgt tatcaaactg catcaggcaa tagcccagca ccagagtgcc
    28321 taacgccatc acgctgaaac caattttcag agccggttta cgacctgctt tatctaccgt
    28381 aaagaccgca atgaaggtcg cgaacataaa ggtcagtccc acgaccagag tcgcaatcat
    28441 ctgttgttct gtggtcgtaa agcccgccat tttgaagata cgcggcgcgt agtacatgat
    28501 gatgttcata ccggtaaact gctgcatcgc ctgcaacaac ataccgagaa acacagcacg
    28561 acggacgtta cggttgatct taaacagtgc ccaaccgccc tgttttaact tcaggctttc
    28621 acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac
    28681 ttcttccgcc tcaatatgac gccccttttc cgccagccag cgcgggctat ttggcaggaa
    28741 gaccaccaga ataatcagca gaactgctgg taaagcaaga acccccaaca ttgcgcgcca
    28801 gttaccgcta taactgaacg ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac
    28861 catcaactgg tacatactga tcatcttacc gcgaacgttt tcacttgcca tttcagaaag
    28921 atacagagga gcggtgtaag acgcgatccc gacagcaatg cccagcacca cacgagcggc
    28981 gattaacatc tctacgctgg tcgcaaaagc ggaccctata gaaccgagta caaacaggat
    29041 ggcccccgcc atcaggctgt atttacgccc caggcggaac gacagccaac cattaaacag
    29101 cgcaccaatt gctgcgccga gcatcatgct actaaccacc cattcctgca aacgactggt
    29161 cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg attacgccga tatcaagacc
    29221 aaataacaat cctgcgaccg cagcagctac cgaaacaaac atattcatac gccgcgtatc
    29281 acgcaaagaa cgtggcgtta aagcagattc cgtattgata gtaaccattt tttcctgcca
    29341 gcagagagta agacatagtg aaaaaatacg tgaacaattc acacaggtgt caggtcggaa
    29401 acagcataaa tatggattaa attgctgcga catgccgtta tgtgatggat attccaattt
    29461 taaaattaag ttgaattatt gagattatta ttaaccacct aattttaaag cagataaaat
    29521 tcataaagtt cattaattga taattgatat ggattatgat gatctagaga tataaaaaac
    29581 cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca gccaaccgcc
    29641 atccacagca atggtataac catttacata atctgaagcg ctggaggcaa gaaacactac
    29701 cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcgat cgagaatttc
    29761 cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg
    29821 agctatcgca ttaacattaa tgttgtgttt agcccattcg tttgccatca atcgcgtcac
    29881 acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga
    29941 gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa agtgtttcgc
    30001 cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc
    30061 tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat
    30121 atcaatatga ccaaattcag ctaccgcgcg atccagcagt ccaggaatgc catcaatctt
    30181 tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gctcgatggt
    30241 ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa
    30301 cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt taccttggag
    30361 agaaaatgca cttaaaatca taacaacacc ttattcgttt atgcccacaa ctagcgcaaa
    30421 tctttaacgg ccacatggtc catatcatta aagacctggt tttcaccgac catgccccag
    30481 ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctcgg ggagatcacc
    30541 gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa
    30601 acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgggtg
    30661 tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat
    30721 tgacaggttt ccagtacatc cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg
    30781 aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtgggatac
    30841 gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtggcggt atcaatactg
    30901 gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg accgatttca
    30961 tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg
    31021 cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc
    31081 gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg
    31141 gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag cccttgggta
    31201 tccagcgttt ttgcgtgcgc accgtggatg ctctgtctta cgtccacatc aacctccgat
    31261 aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa
    31321 gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt ttcattttta
    31381 agttgaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg
    31441 agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg
    31501 gcagatattt atctgtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc
    31561 cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac
    31621 catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag
    31681 tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac
    31741 cgaaagcgcc tgtgcagcaa ccgcttcatt agcctcgata agatcgacat cagccaactg
    31801 ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc
    31861 ccaccaacgc tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgagct
    31921 ttggcttcgc tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc
    31981 gcggtcacag aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg
    32041 gcgtcagtgc gtggctgttc atcggtatca acaaccaacg tctgaccgtt actttggctc
    32101 attaccggga cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt
    32161 tgcgagctaa gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga
    32221 ttttcggcgg tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc
    32281 agactgtcaa ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca
    32341 tgtggtgcgc ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac
    32401 tgtatcgcct gagtagccag atgcagtgct ttaagaccgg aaccgcaaac gtcattaatc
    32461 gtgattgcag aaacgctgtt aggaagacca cctttaatag ccgattgcct tgccggattc
    32521 tgccctgccc ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga
    32581 acgccggtac gttctattaa cgctttcacg accagactac caagttccac ggcagaatga
    32641 cccgctaacg caccacgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg
    32701 tctttcatca caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt
    32761 tatcttaatt gtttaaaaaa agtgattttt atcacaaagg aaatatgcct gagcagtagt
    32821 cagagtcata actggcacgt aaggtttgca accactaacc caccaataga tgggtagata
    32881 gggcgttaat ctcccatact taacttggtt tatggtaaat tgccctccat tttgtttaat
    32941 ttgtagatga tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtggag
    33001 tttcgcgctc tacggcacag ccgttggtgc aggcacgcta ttccttccta ttcagttagg
    33061 ttcggcaggg gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg
    33121 gccacataaa gccttatgcc agttcatcct ctcatcaaaa acatcagcag gtgaagggat
    33181 aacgggcgcg gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta
    33241 cttcatcgcc ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac
    33301 ggaacagctg gcaaagcata tggttattga tctttgcatc cgtatgttgg tgagtctggg
    33361 tgttgtatta attctgaatc tcatttttct gatgggacgt catgccacta ttcgggtaat
    33421 gggatttttg gtattcccat tgattgccta tttcttattt ctttccattt accttgtcgg
    33481 tagttggcaa cctgatctat taacaaccca ggtagagttc aatcagaata cccttcacca
    33541 gatatggata tcgattcccg tgatggtttt cgcctttagc catacgccca ttatttctac
    33601 gtttgccata gacagacgtg aaaaatatgg cgaacacgct atggataaat gcaaaaaaat
    33661 tatgaaagtc gcttatctca tcatctgcat aagtgtactg ttctttgtct ttagctgcct
    33721 gctttctatt ccatcttcgt atattgaagc ggctaaagaa gaaggggtca ccattttatc
    33781 ggcgctttct atgctgccga acgccccagc atggttgtca atttccggga ttattgtcgc
    33841 agtagttgcg atgtcgaaat cattcctagg tacgtacttt ggcgttattg aaggtgccac
    33901 agaggtcgtc aaaacaacac tacagcaggt tggtgtaaag aaaagtcgtg catttaacca
    33961 cgcactatca attatgttgg tatcgctgat taccttcatt gtttgttgca ttaacccgaa
    34021 cgcgatttcg atgatttacg cgatcagcgg cccgctcatt gccatgatac ttttcatcat
    34081 gcctacgctg tcaacgtatc tcatcccggc gcttaaaccc tggcgttcca tcggaaatct
    34141 gattacgctg atcgtgggta tcctgtgcgt atcggtaatg ttctttagct aatatccgtt
    34201 gtggatggtt caaaa
//
LOCUS       DADLSE010000031        34180 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLSE010000031 DADLSE010000000
VERSION     DADLSE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.031"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(78..479)
                     /gene="nikR"
                     /locus_tag="JN692_001453"
     CDS             complement(78..479)
                     /gene="nikR"
                     /locus_tag="JN692_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7263798.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(485..1291)
                     /gene="nikE"
                     /locus_tag="JN692_001454"
     CDS             complement(485..1291)
                     /gene="nikE"
                     /locus_tag="JN692_001454"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7263799.1"
                     /translation="MSLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(1288..2052)
                     /gene="nikD"
                     /locus_tag="JN692_001455"
     CDS             complement(1288..2052)
                     /gene="nikD"
                     /locus_tag="JN692_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7263800.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2052..2885)
                     /gene="nikC"
                     /locus_tag="JN692_001456"
     CDS             complement(2052..2885)
                     /gene="nikC"
                     /locus_tag="JN692_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7263801.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRMSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYSRMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2882..3826)
                     /gene="nikB"
                     /locus_tag="JN692_001457"
     CDS             complement(2882..3826)
                     /gene="nikB"
                     /locus_tag="JN692_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7263802.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3826..5400)
                     /gene="nikA"
                     /locus_tag="JN692_001458"
     CDS             complement(3826..5400)
                     /gene="nikA"
                     /locus_tag="JN692_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7263803.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADN
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5511..6098)
                     /gene="acpT"
                     /locus_tag="JN692_001459"
     CDS             complement(5511..6098)
                     /gene="acpT"
                     /locus_tag="JN692_001459"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7263804.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6153..7202)
                     /locus_tag="JN692_001460"
     CDS             complement(6153..7202)
                     /locus_tag="JN692_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7263805.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RNLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7301..8551
                     /locus_tag="JN692_001461"
     CDS             7301..8551
                     /locus_tag="JN692_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263806.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPEHVPGAASSS"
     gene            complement(8555..9112)
                     /gene="dcrB"
                     /locus_tag="JN692_001462"
     CDS             complement(8555..9112)
                     /gene="dcrB"
                     /locus_tag="JN692_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7263807.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9185..9850)
                     /locus_tag="JN692_001463"
     CDS             complement(9185..9850)
                     /locus_tag="JN692_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7263808.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQRFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10071..10316
                     /gene="tusA"
                     /locus_tag="JN692_001464"
     CDS             10071..10316
                     /gene="tusA"
                     /locus_tag="JN692_001464"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7263809.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10418..12616)
                     /gene="zntA"
                     /locus_tag="JN692_001465"
     CDS             complement(10418..12616)
                     /gene="zntA"
                     /locus_tag="JN692_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7263810.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTVNALRLLRRR"
     gene            complement(12690..13316)
                     /locus_tag="JN692_001466"
     CDS             complement(12690..13316)
                     /locus_tag="JN692_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7263811.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13457..13816
                     /locus_tag="JN692_001467"
     CDS             13457..13816
                     /locus_tag="JN692_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7263812.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13819..14088)
                     /locus_tag="JN692_001468"
     CDS             complement(13819..14088)
                     /locus_tag="JN692_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7263813.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14078..14674)
                     /gene="rsmD"
                     /locus_tag="JN692_001469"
     CDS             complement(14078..14674)
                     /gene="rsmD"
                     /locus_tag="JN692_001469"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7263814.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            14824..16320
                     /gene="ftsY"
                     /locus_tag="JN692_001470"
     CDS             14824..16320
                     /gene="ftsY"
                     /locus_tag="JN692_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7263815.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16323..16991
                     /gene="ftsE"
                     /locus_tag="JN692_001471"
     CDS             16323..16991
                     /gene="ftsE"
                     /locus_tag="JN692_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7263816.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            16984..18042
                     /gene="ftsX"
                     /locus_tag="JN692_001472"
     CDS             16984..18042
                     /gene="ftsX"
                     /locus_tag="JN692_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7263817.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18287..19141
                     /gene="rpoH"
                     /locus_tag="JN692_001473"
     CDS             18287..19141
                     /gene="rpoH"
                     /locus_tag="JN692_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7263818.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19412..20501
                     /locus_tag="JN692_001474"
                     /pseudo
     CDS             19412..20501
                     /locus_tag="JN692_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
     gene            complement(20700..21083)
                     /gene="panM"
                     /locus_tag="JN692_001475"
     CDS             complement(20700..21083)
                     /gene="panM"
                     /locus_tag="JN692_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7263819.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTALQGGWEKR"
     gene            21507..22616
                     /gene="livk"
                     /locus_tag="JN692_001476"
     CDS             21507..22616
                     /gene="livk"
                     /locus_tag="JN692_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7263820.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLLNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            22664..23590
                     /gene="livH"
                     /locus_tag="JN692_001477"
     CDS             22664..23590
                     /gene="livH"
                     /locus_tag="JN692_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7263821.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            23587..24864
                     /locus_tag="JN692_001478"
     CDS             23587..24864
                     /locus_tag="JN692_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7263822.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGRDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            24861..25628
                     /gene="livG"
                     /locus_tag="JN692_001479"
     CDS             24861..25628
                     /gene="livG"
                     /locus_tag="JN692_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7263823.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            25630..26343
                     /gene="livF"
                     /locus_tag="JN692_001480"
     CDS             25630..26343
                     /gene="livF"
                     /locus_tag="JN692_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7263824.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            26740..28056
                     /gene="ugpB"
                     /locus_tag="JN692_001481"
     CDS             26740..28056
                     /gene="ugpB"
                     /locus_tag="JN692_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7263825.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNLGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            28154..29041
                     /gene="ugpA"
                     /locus_tag="JN692_001482"
     CDS             28154..29041
                     /gene="ugpA"
                     /locus_tag="JN692_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7263826.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            29038..29883
                     /gene="ugpE"
                     /locus_tag="JN692_001483"
     CDS             29038..29883
                     /gene="ugpE"
                     /locus_tag="JN692_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7263827.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            29963..30850
                     /gene="ugpC"
                     /locus_tag="JN692_001484"
     CDS             29963..30850
                     /gene="ugpC"
                     /locus_tag="JN692_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7263828.1"
                     /translation="MADGEFIVMVGPPGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMNVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            30872..31615
                     /gene="ugpQ"
                     /locus_tag="JN692_001485"
     CDS             30872..31615
                     /gene="ugpQ"
                     /locus_tag="JN692_001485"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7263829.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(31602..32042)
                     /locus_tag="JN692_001486"
     CDS             complement(31602..32042)
                     /locus_tag="JN692_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7263830.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            32162..33904
                     /gene="ggt"
                     /locus_tag="JN692_001487"
     CDS             32162..33904
                     /gene="ggt"
                     /locus_tag="JN692_001487"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7263831.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAAPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(33963..>34180)
                     /locus_tag="JN692_001488"
     CDS             complement(33963..>34180)
                     /locus_tag="JN692_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7263832.1"
                     /translation="NRGVASALMREMIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFG
                     FEIEGTGKKYALRNGEYVDAYYMARVK"
BASE COUNT         7920 a         8914 c         9545 g         7801 t
ORIGIN      
        1 atctatctat gcaactgaac ttaattttat aaattaagtt cagttaatat cttttctttc
       61 cttgttttag aaataactca atcttccttc ggcaggcact gcaaatgccc gtgccgcacg
      121 ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt cacccatgtc acctttcaac
      181 acggcgattt ccagacagtc gtcgtggttg atgtgcacat gtagcgtggc gacggagagg
      241 tcgtggtgat gatgctgggt ggaaacaata cggctggcta agtcgcgttt ttcgtgttca
      301 tacacatacg acagcaccgc gaaaccttgt gtgccgtgct gctgggtagc ttcctgcgcc
      361 agggcactac gcagaatatc gcggatagct tcggaacggt tgttataacc acgacgctgg
      421 ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt
      481 ggcgttaaac cttttctgag gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt
      541 gtagcacacg tccggcgtca gaggaaaagg ttaatttatc tcccaccgcc tgggtttcga
      601 cgatttgtcc gttgtccatc accatcactc gctggcaaaa gcgttccacc aggcgtaagt
      661 catgagtgat gaacaggcag gcagtgccaa actgttgttg tagctttttc agcaggcgaa
      721 tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagcaatt
      781 tcggttcgac cgccagcgcg cgtgccaggc agacgagctg gagctggccg ccgcttaact
      841 gcggtgggcg tttgtcgaga acgctgtcat cgagatcgac cgccttcagc atttcgctgg
      901 cgcgcgccag ttgttcggat tttttcagtg atagcaggtg gcgcatcggt tcacgcagga
      961 tctcgcgcac ggttttgcgc ggattcacgg cactgatgga gtcctgaaac accatctgaa
     1021 tatcacggcg gaaggcttta cgttgggcgc gattaagttt cgccagcgat tcgccgcgcc
     1081 agctaatatt cccttgtgag ggagactcta agcctaccag cagccgcgcg agggtgcttt
     1141 ttccgcagcc gctgcgtccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa
     1201 cgttattcag caccgcctga tgttgatgtt ttccgctaaa tccaccgtgg gcatagtgat
     1261 gggaaaggcc gcagacgtta agtaagctca tgatgccagc tccataccgt agagggcgag
     1321 atgagcggaa accaggctgc gcgtcaccgt atgtttgggg gcgttaaaca gcgtttctac
     1381 atcgccctgt tcgacaattt taccttgtga catcaccgcc acgtcatccg ccagacgcgc
     1441 cacaacgccc atatcatggg tgaccagcag cattcccggc gcttgttttt gcataatgct
     1501 ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg tcggttcatc
     1561 ggcgatgata aacggtgatt cacacagcac cgccatcgca atcatcatgc gctgcaacat
     1621 gccgccgctc atctcgaacg ggtacagctt cagcacgcgc gcggcgtttt ccagccccac
     1681 cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt ttccctaacg ccaggcaggt
     1741 ttcccgcgcg tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat
     1801 gatggtggca attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa
     1861 aatttccccc gccgtctggc gaacgccagc gggcaaaatg cccagcgtcg cggcgcaggt
     1921 taatgatttc ccgctgccgc taccgccgac taacgccagc acgcgcccgc gttgcagggt
     1981 taacgataca ccgtgtacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat
     2041 ctgttgcggc attagtgtgc gtgctccgtc accagatgag gatccagatg atcgcgcagt
     2101 gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac
     2161 atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg
     2221 gcggtcggcg cggtcacacc gaggccaagg aaagacatcc ccgcgacgtg cagcatcata
     2281 tggccgatat ccagcgttgc cagcaccagc agcgaaggga tcaccgcgcc tgccagatga
     2341 tcgacaaaca cccgcacatg gcccgcaccg gaaagccgtg acgccagcac aaactcgcgt
     2401 tggcgtagtg aaatcaccaa gctgcgcacc atgcgtgaat accacgccca gtgcgacagg
     2461 gcgatggcga taattacgtt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac
     2521 agaatcgagg tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg
     2581 cgcccgccaa tcaacccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc
     2641 aggcaggcca ttaccgaacc gagcgacatg cgggtcgctg ccatcagccg cgagaaaata
     2701 tcgcgaccta agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcacgac
     2761 ggcaaatcaa ttgcctgtgg atcgtacggc agccaccact ggctggtgag cgcaatcagc
     2821 gccagcaggg cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag
     2881 ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat
     2941 cgacaatcaa attacagacc acaaaaacca ccaccatcat cagcgtaaag cactgaatca
     3001 ccggatagtc acggttaaaa atcgccgaca ccgcatagcg cccgacgcca ggccaggcaa
     3061 agatgttttc gataatcatc gtcccgccaa tcaattcgcc aatgtgcatc cccacggcgg
     3121 tgatcatcgg cagcgaggca ttgcgcagaa tatggcggcg ttcggtctgt ttgtcattca
     3181 ggccgcgtag ccgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc
     3241 gcagcagtcg cgcgttaatc gccagcgaca taaaggcgat ggaaaccgca ggtaaaatga
     3301 tgtgctgcca gccgccgtag cccatcgcgg gtagccattg cagatacacc gaaaacgcca
     3361 tcactagcaa aaatgccagc cagaagttgg gcatcgacac gccaagaaac gcgataaagc
     3421 gcacggcgaa atccggcaaa cgatcgcggt ggcgcgccgc ccagataccg agcggtacgg
     3481 aagtgagcag aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt
     3541 tcagcatatc gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca
     3601 gcgccttcca caaccaggta ccgtactgga cgtacagcgg ctgatccagc ccgagcatgg
     3661 tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt agataaacgc agataatcga
     3721 gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca
     3781 ccatcgggat cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg
     3841 tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg taggggatat tacccagctc
     3901 cggttttgat accaccatca tcgagatgta gctgataggc agataaaccg cctcgtcatg
     3961 cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag
     4021 cacttcgccg atctctttat caatcagcgg gttgtcggct aatccttgct gtgcctggaa
     4081 gtcagcgtgc gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc
     4141 ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct
     4201 ctcttcttcg ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat
     4261 ttccgccatc gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg
     4321 ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag
     4381 caactctttc gctttctgcg gatcgtactg gcgcggtttc aggccgagat tagcgtaagg
     4441 cacagaaggg gcaaacaggg tgtcggcgac ctgctgggtg ccatataacg cgttatcaat
     4501 cagcgatttt ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg
     4561 ggctttggcg gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg
     4621 gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc
     4681 gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat
     4741 gacgttaaag gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac
     4801 gaagacatcg tactgattca gtttcgattc ctgcaaaatc cacggtccgg tgccaatcgg
     4861 cgctttaatt ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa
     4921 aggacggggc agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg
     4981 cagctctgtt ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc
     5041 gtgacgttga cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa
     5101 cggttcaccg ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc
     5161 ttctgaatga gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt
     5221 caccaatggt tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg
     5281 gtttagtggc ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg
     5341 gacgataaaa gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat
     5401 ggtaacccca atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt
     5461 catactgatt acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact
     5521 gaatcgatcc attgcacact gtcggcggtg agcgtaaagg gggtgggggt gcagatcgca
     5581 aggcttaaat tttctagctg acaatggctg actgacagcg aggagtgata ggtgctatct
     5641 acgctgacga tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc
     5701 cagatgcgcc agaacatttc cagttgctga tcaggatgca cggcgtccat ttcagcgtgt
     5761 tccccgaggc tgaacacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact
     5821 tcgatatcgc agccgacttc gccttcatcg ctcaacagca gagcgatatc gtcaccgcta
     5881 tggcttaagt tgaaccatag cggcgtttcc ggcgcgaatg caggtttgcc ttgttcgcca
     5941 tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag
     6001 cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg
     6061 cttaaggtcg aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga
     6121 aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg
     6181 cccggcccca gtaaaatcgc cagtttgctg ccacctttcg tcgtttccat ccagatttta
     6241 cacacgctgg ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccag
     6301 attaacaatg aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt
     6361 ggttctaaaa tattgccgat gaccatatgg accactaaaa acaatgcgcc gaccagaata
     6421 cattcgtaaa caccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc
     6481 gcgccgatat tgggcacgta gttgagcaaa aacgccagta ctgcccacat cagcgcaaac
     6541 tgtacgccca ttagcgccag ccccagccag acgatgacac ctgtccataa actgagtagc
     6601 gtctttaacg ccagataatg cgaaacgcct ttaagtgcgc ggtgtaatcc tgcgatgtga
     6661 atctgtgggt tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc
     6721 ataaaaacta cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc
     6781 attagcgctg tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc
     6841 ggcgacatat gcaaattaag aaaaggcaac atctcctgca atttaaaaag gttgcgcgtc
     6901 agctccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
     6961 ccgaccagcg cggttagtgc gatcagcatc accaccacta caatcgtaat ggcaacgggg
     7021 cgttgtactc cccgacgaat aaaccaggta acgagcgggt tgaggacgat ggcaaaaaac
     7081 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
     7141 accagcgagg ccaacttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
     7201 attggggctt ccttgtgact ttttgtgtta agtgtagcgg gagtcacgcc agcaatattc
     7261 tgattcttac tgctgatttt cacgtcagca catggtaaaa atgaaacact gttgtaaaaa
     7321 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
     7381 tttgcgcatt gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
     7441 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgcct tctgggcagg
     7501 attggttatc agcctgcaat atttcgccac cttgctgagc cgccctcatg ccggacgtta
     7561 cgccgatttg ctgggaccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
     7621 cggtctgggg tatctgacgg caggattaac cgccagtctg cctgtcatca gcctgttatt
     7681 actttgcctg gggcgcgtca tccttgggat tgggcaaagt tttgccggaa cgggatcgac
     7741 cctatggggc gttggagtgg ttggctcgct gcatatcggg cgggtgattt cgtggaacgg
     7801 cattgtcact tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg
     7861 gggcggcttg caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt
     7921 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
     7981 gcttgggcgc gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt
     8041 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
     8101 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
     8161 ccgtatcggt ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
     8221 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
     8281 cgggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
     8341 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
     8401 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
     8461 ggcgggactg gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
     8521 acacgtccct ggggccgcct catcatctta aaacttactg aataaccagc gtattaatga
     8581 tgttttctgc ggtggtttgc gctttttgct gatcgtcagc tggcagcgta atttgcatgg
     8641 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
     8701 cggagataat actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg
     8761 ttaccacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccaaca
     8821 ccgccagatc ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
     8881 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
     8941 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
     9001 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
     9061 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
     9121 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
     9181 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca
     9241 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
     9301 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
     9361 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
     9421 tcggtgccag ccaccagcgg cgactctgac gcaggcggtt aaaaacgtgc acgtcgagga
     9481 tttgccccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
     9541 agtgggcgag tgcgccgaat ctctgccagg aacccatata gaatagcgac gagatgacgt
     9601 aggagattaa taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc
     9661 caaaaatacg cacggtcagg tcggtagcaa gaaaaataaa cggaaagcta aacgcgcccc
     9721 aggtggtatg gaaaccaaaa atggagacgg gaagctgaac cagatagtta ctggaggtga
     9781 tcaccagaag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
     9841 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
     9901 cacgtctgtt accttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
     9961 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaaccta gtttgcttac
    10021 ggactcacag ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc
    10081 tcttttccag ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga
    10141 tgatggtgcg caaaaccgtg cgcaatatgc agcctggcga aacgttgctg attatcgccg
    10201 acgatccggc cactacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
    10261 ttgcgaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatagg
    10321 ggctgattgg cttcgatgcc gccttttcct caccccctaa ccctctcccc agaggggcga
    10381 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaacctta
    10441 acgcattcac cgtcaccagc accgtcgcac ccgtatctgc cagcactgcc agccacaacc
    10501 cggtcatccc taaaagcgtg gtgacgagga agatcccttt cagtcccagc gcaatggtga
    10561 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc
    10621 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccgctac
    10681 ccatagcaat cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga
    10741 ccatcgccag gggcgcatgt tgattcagct tggtcaccgc tttgacttta tcttccggca
    10801 ataggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
    10861 tatcgccggt gaggatcact cctttaaccc ccagcgcgtt cagttcactg atggcagttg
    10921 cagcgtcggc gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta
    10981 ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat
    11041 cagcgggatg tttcccggca gcgcatatca acacgcgctc accattaacc tgcgcttcaa
    11101 tgccagaccc gaccagtgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct
    11161 gtgcttcgcg tacgatggct tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
    11221 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    11281 gtttaccgac ggtcagcgta ccggttttat caaacgccac ttgggtaaca cgacctagtt
    11341 gttccagcgc cgcaccgcct ttaattaacg ccccacgacg cgctgctgct gccagcccgg
    11401 aggttatcgc cgcaggcgtt gagataacta acgcacacgg gcaaccaatc agcagcagcg
    11461 tcagaccttt ataaatccac tcctgccagc tggcggcaaa cagcagcggc ggcactagcg
    11521 ttaccagcag ggcgacggcc ataatcgctg gcgtataaat acggctgaaa cggtcgataa
    11581 accgctcaat gggggcgcgg cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    11641 tggcgctggc acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg
    11701 ctcctgcagg aactttatcg cccgtcgcgc gctccaccgg aatggattct ccggtcaggg
    11761 cgctttcatc aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgcccac
    11821 ctgcggcgac ttcaatcaca tcgccaggcc gcaggctgtt aatcgccacc tcttcccgct
    11881 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctaacgccct
    11941 gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca
    12001 ccatcgcagc ttcagccgtt gcgccaataa acagtgcacc aatagcggct acgctcatta
    12061 aggtttcaat ggcgaagtag ctgccggatt tgatcagccg taatgcctga cgagcaatcg
    12121 ggtacagccc aactagcgtg gtcgcgataa acgccagttg cccgaacgga tgattgaact
    12181 gctccagacc ccagctgatt gccatcatca cgataagcgt aatcagcggc agattctctt
    12241 tcaggcgcga tgcttgtggc tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt
    12301 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatcaacc accagttttt
    12361 cggtggcgaa caacacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta
    12421 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    12481 agacgttttc agagagcgtt ggcgagctgg agcatgcgcc gtcgcagcaa cagtcgttgg
    12541 cgttctgtac cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
    12601 tgtcaggtgt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    12661 tctggagtcg actccagagt caagttttat cagagataca gtgagcggac gatgaggaag
    12721 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
    12781 tggctcccca gccagacaaa gttactgata aacagcaacg aagcgccaac aaacgcagag
    12841 agcgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    12901 gtcatgccga taaacgtgca gataggccaa cggtactctt ccagccgcgt ccagataatc
    12961 gccagtagca gcgcacccag aaccagcaac accagtggca gaggccagaa gaaagagagc
    13021 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    13081 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    13141 agtcccgcca gcaccagata gctaatggcg tcgaacatcg gtgcttgcca ggccagtaac
    13201 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
    13261 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    13321 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    13381 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt
    13441 ccgatgaggg ataaagatga gtaaaccacc tcttttcttt attgttatca ttggcttaat
    13501 tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
    13561 ggctccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
    13621 tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
    13681 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca
    13741 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgttagctt
    13801 tgttggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    13861 aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt
    13921 cggtaaagtg gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag
    13981 cgccacgtta acgaaaatat ttagcgggcg cgggaaggga tgcaccagat tgaggattaa
    14041 aaatccccaa acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    14101 gtgcttcgtg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    14161 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    14221 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    14281 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg
    14341 acattgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    14401 ttaactgctg agaaaccacg cgatccatct caatcaacgt tgcccccgca gcgtagcgcg
    14461 ataacgcttc cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa
    14521 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    14581 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    14641 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    14701 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    14761 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
    14821 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    14881 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    14941 caggcgcaag agcctgtgaa ggcttctgaa caagccgttg aagagcagcc gcaggcgcat
    15001 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgtt
    15061 gaaagtgaaa aagcgcagcc tgaagcggaa gttgttgcac agccggaacc ggtcgtagaa
    15121 gaaacgccgg agccagtggc tatcgaacgt gaagagctgc cgttgccgga agacgtcaac
    15181 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    15241 gcggcagaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    15301 ctggctgccg aagcggcaga agaggcggtg atggtagttc ctccggcaga agaagagcag
    15361 ccggttgaag aaattgccca ggagcaagaa aaaccgacca aagaaggctt cttcgcgcgc
    15421 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
    15481 cgcggtaaaa aaatcgacga tgatctgttt gaagagctgg aagaacagct gttgatcgcc
    15541 gatgtgggcg tggaaaccac acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc
    15601 aagcagcttc gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt
    15661 ctggcgaaag tcgatgagcc gctgaatgtt gaagataaaa cgccgttcgt gatcctgatg
    15721 gtgggcgtca acggtgtggg taaaaccacg acgattggta agctggcgcg tcagtttgag
    15781 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc tgcggttgaa
    15841 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
    15901 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
    15961 ctgattgccg ataccgccgg acgtctgcag aacaaatcgc atctgatgga agagttgaag
    16021 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
    16081 attgatgcca gcaccgggca gaacgcggta agccaggcca aactgttcca cgaagctgtt
    16141 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
    16201 tcggtggctg accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag
    16261 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
    16321 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
    16381 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
    16441 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
    16501 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgtcgcc
    16561 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
    16621 tggcgatccc gctgattatc gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
    16681 cggcactgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttccg
    16741 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc
    16801 tggcggacga accgaccggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
    16861 ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc aacgcacgac attaacctga
    16921 tttcacggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    16981 gccatgaata agcgcgatgc aatcaatcat attcggcagt ttggtgggcg tctggatcgc
    17041 ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta acgcaccgaa acgcgcgaaa
    17101 tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc
    17161 ttccacggcg cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg
    17221 gttatcgcca tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaat
    17281 caggcggcga cgcagtatta tccgtcaccg caaatcactg tttacttgca aaaaacgctg
    17341 gacgatgacg ccgctgcggg cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa
    17401 gtgaactatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    17461 ggtgcgctgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    17521 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    17581 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    17641 ctggttgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    17701 atcggcaaca gcgtacgtct gagcatcttt gctcgccgtg actccatcaa cgtgcagaaa
    17761 ctgattggtg cgacggatgg tttcatcctt cgcccgttcc tgtacggcgg tgcgctgctg
    17821 ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa ttctggtact gcgtttgtcg
    17881 tcagcggtgg cggaagtggc acaggttttc ggaacgaagt ttgatatcaa tggtttatcg
    17941 ttcgatgaat gcctgctatt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    18001 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    18061 tttccctgca atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc
    18121 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    18181 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    18241 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    18301 gcaaagttta gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc
    18361 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    18421 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    18481 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    18541 cctgatgaaa gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    18601 cgtccactgg atcaaagcag agatccacga atacgttctg cggaactggc gtatcgtcaa
    18661 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    18721 tctgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    18781 caaagacgta cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    18841 ttccgacgac gattccgaca gccagccgat ggcaccggtg ctctatctgc aggataaatc
    18901 atctaacttt gccgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct
    18961 gaccgacgcg atgcatggtc tggacgaacg cagccaggac atcatccgtg cgcgctggct
    19021 ggacgaagac aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga
    19081 acgtgtgcgc caactggaaa agaacgcgat gaaaaaactt cgcgccgcta ttgaagcgta
    19141 atttccgcta ttaagcagag aaccctggat gagagtccgg ggtttttgtt ttttgggcct
    19201 ctacaataat caattccccc tccggcaaaa cgccaatccc cacgcagatt gttaataaac
    19261 tgtcaaaata gctattccaa tatcataaaa atcgggtatg ttttagcaga gtatgctgct
    19321 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aacaacaaca tcacaacaca
    19381 cgtaataacc agaagaatgg ggattctcag gatgaacata aagggtaaag cgttactggc
    19441 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    19501 cgtgggtgca atgtccggtc cggttgcgca gtacggtgac caggagttta ccggcgcaga
    19561 gcaggcggtt gcggatatca acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt
    19621 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    19681 cgacggcatt aaatatgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    19741 catctacgaa gacgaaggca ttttaatgat caccccagcg gcaaccgcgc cggagctgac
    19801 cgcccgtggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    19861 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    19921 acagcaatac ggcgaaggtc tggcacgagc ggtgcaggac ggcctgaaga aaggcaatgc
    19981 taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa gatttctcaa cgctggtggc
    20041 gcgtctgaaa aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg
    20101 gcaaatcctg cgtcaggcac gcgcggcagg gctgaaaact ggaaggtgtg gctaacgttt
    20161 cgctgtctaa cattgcgggc gaatcagcgg aagggctgct ggtgaccaag ccgaagaact
    20221 acgatcaggt tccggcgaac aaacccattg ttgatgcgat caaagcgaaa aaacaggacc
    20281 caagcggcgc attcgtctgg accacctacg ccgcgctgca atctttgcag gcgggcctga
    20341 atcagtctga cgatccggct gaaatcgcca aatacctaaa agcgaactcc gtggataccg
    20401 tgatggggcc actgacctgg gatgagaaag gcgatctaaa gggctttgag ttcggcgtat
    20461 ttgactggca cgccaacggc acggcgaccg atgcgaagta attgttaatc ggcaactttg
    20521 ggttgccgcc aaattgctaa tatcgaatac gttgcttcat gccggatgcg gcgtaaacgc
    20581 cttatccggc ctacaagatc caaagaaatc agtaaattgc agcacacatt ataggcctga
    20641 taagcgtagc gcatcaggca atttctcgtt ggtcatcact tttgaaatca acttgacgat
    20701 taacgcttct cccagccgcc ctgcagtgcc gtaaatccca gtgcctgcat aaacgccgtc
    20761 atcacaccgc gatcttccac gcctgcatcc gccatccacc agcatgaaac gccaggattg
    20821 ttacgcaaaa cctcttccag cagatattgc cccacaccgc gacggcgggt gacttcccgc
    20881 acgcgtagag aatccagtgc tccctcggtg ccgcttaagg ttacccgcac ggccgcaagt
    20941 agacgctcgt taaaacgcgc ggcgtagata cggtggttat cgtcaacctg taaagaggaa
    21001 ggggaatact ccggccagat cttttgcagg tcaatccggt cctggtcgct aaagttttct
    21061 aatcgaatga tggtcagctt catgggtaac ccgtgtaaat cacaaaagtg gaaccagtgt
    21121 agcgaaataa tttaatcgga ggctttctct tttttatttc ttttggcagg tgattaattt
    21181 tttaacagca ataattacaa agttaaaaca ttagagaatg aaaaatgtcc agcataatcc
    21241 cctgaatgat agtgaattat tccgcccttt gtgccgttat tttatgctga caaaggcact
    21301 tttttctgtt tatctatcaa taaattcaga atattatctg ttcttaatcg actgaaaaat
    21361 agggatttta atcgctatca tcacaaaata ctgcgctaac cccttaatca gacaggcaaa
    21421 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
    21481 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
    21541 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
    21601 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
    21661 cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat
    21721 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaacgacg
    21781 gcattaaata cgttattggt catctgtgtt cttcctctac ccagccagcg tcggatatct
    21841 acgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
    21901 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg cctacggcgg
    21961 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
    22021 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
    22081 tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc
    22141 tgaaaaaaga aaatatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
    22201 tgctgcgcca ggcccgttcc gttggcctga aaacccagtt tatggggccg gaaggtgtcg
    22261 gtaacgcatc gctgttgaac attgccggtg atgccgccga aggcatgttg gtcactatgc
    22321 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
    22381 agaaagatcc gtccgggcct tatgtctgga tcacttacgc ggcagtgcaa tctctggcaa
    22441 ctgcccttga gcgtaccggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
    22501 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
    22561 ttgattttgg tgtcttccag tggcacgccg acggttcatc cacggcagcc aagtgatcat
    22621 cccaccgccc gtaaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
    22681 gtatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
    22741 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
    22801 ttatatgatt ggcagctacg tctcatttat gatcatcgcc gcgctgatga tgatgggcat
    22861 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca tcgccagcgc
    22921 ctacggctgg agtatcgaac gggtagctta ccgcccggtg cgtaactcta agcgcctgat
    22981 tgcgcttatt tccgcaatcg gtatgtccat cttcctgcaa aactacgtca gcctgaccga
    23041 aggttcgcgc gacgtggcgc tgccgagcct gtttaacggt cagtgggtgg tgggccatag
    23101 cgaaaacttc tctgcctcta ttaccaccat gcaggcggtg atctggattg tcaccttcct
    23161 cgccatgctg gcgctgacga ttttcattcg ctattcccgt atggggcgcg cctgtcgtgc
    23221 ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc
    23281 tttgaccttt gttattggcg cggcgatggc ggcagtggct ggcgtactgc tcggtcagtt
    23341 ctactgcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
    23401 ggtgctcggt gggattggca gcattccagg ggcgatgatt ggcgggctga tcctggggat
    23461 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc
    23521 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
    23581 gaaagtatga aaccgatgca tattgcaatg gcactgctct ctgccgcgat gttctttgtc
    23641 ctggcgggcg tctttatggg cgtacaactg gagctggatg gcaccaaact ggtggtcgac
    23701 acggcttcag acatccgttg gcagtggatg tttatcggca cggcggtggt ctttttcttc
    23761 cagcttttgc gaccggcttt ccagaaaggg ttgaaaagcg tttccgggcc gaagtttatt
    23821 ctgcccgcga ttgatgggtc cacggtgaag caaaagctgt tcctcgtggc gctgctggtg
    23881 cttgtggtgg cgtggccgtt tatggtgtca cgtgggacgg tggatatcgc cactctgacc
    23941 atgatctaca ttatcctcgg tctcgggctg aatgtggttg ttggtctttc tggtctgctg
    24001 gtgctggggt acggcggttt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
    24061 tattacggtc tggggttctg gacctgtttg ccgattgccg gactgatggc agcggcggcg
    24121 ggtttcctgc tcggttttcc ggtgctgcgt ctgcgcggtg actatctggc gatcgttacc
    24181 cttggtttcg gcgaaattgt gcgcatattg ctgctcaata acactgaaat caccggcggc
    24241 ccgaacggaa tcagtcagat cccgaaaccg acactcttcg gactcgagtt cagccgtacc
    24301 gctcgtgaag gcggcaggga cacgttcagt aatttctttg gcctgaaata cgatccgtcc
    24361 gatcgcgtta tcttcctcta cctggtggcg ttgctgctgg tggtgttaag cctgtttgtc
    24421 attaaccgcc tgctgcggat gccgctgggc cgcgcgtggg aagcgttgcg tgaagacgag
    24481 atcgcctgtc gttcgctggg cttaagcccg cgccgtatca agctgaccgc ttttaccatc
    24541 agcgccgcat ttgccggttt tgccggaacg ctgtttgcgg cgcgtcaggg ctttgtcagc
    24601 ccggaatcct tcacctttgc tgaatcggcc tttgtgctgg cgatagtggt gctcggcggt
    24661 atgggctcgc aatttgcagt gattctggcg gctgttctgc tggtggtgtc gcgcgaattg
    24721 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
    24781 atctggcgtc cgcaggggtt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
    24841 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgttaacggt ctgatgatgc
    24901 gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggaaatcg
    24961 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgc ctgaccggtt
    25021 tctacaaacc caccggcggc accattttac tgcgcgatca gcatcttgaa ggtttaccgg
    25081 ggcagcaaat tgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgttccgtg
    25141 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca gcaactgaaa accgggctgt
    25201 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg
    25261 ccgccacctg gcttgagcgc attggcttgc tggaacacgc caaccgtcag gcgagtaacc
    25321 tggcctatgg tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
    25381 ttttaatgct cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg
    25441 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
    25501 tgaagctggt gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg
    25561 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
    25621 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
    25681 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt cactctcatt
    25741 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgcc
    25801 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
    25861 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
    25921 gaagagaacc tggcgatggg cggcttcttt gccgaacgtg accagtttca ggagcgcata
    25981 aagtgggtgt atgaactgtt tccacgtctg catgagcgcc gtgttcagcg ggcgggcacc
    26041 atgtccggcg gtgaacagca gatgctggcg attggtcgcg cgctgatgag caatccgcgt
    26101 ttgttgttac ttgatgagcc atcgctcggt cttgcgccga ttatcatcca gcaaattttc
    26161 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
    26221 aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc
    26281 tccgataccg gcgacgcgct gctggcgaac gaagcagtga gaagtgcgta tttaggcggg
    26341 taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa
    26401 aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg
    26461 cacgatcttg taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgagtaat
    26521 ctgtagagtt tgattcagac cttctatttt tccgcttatc cgtgccccat ctcccatttt
    26581 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttactatc ttacaaatgt
    26641 aacaaaaaag ttatttttct gtaattcgag catgtcatgt taccccgcga gcataaaacg
    26701 cgtgaattcg cgcattcggt acaacaagag agataaacga tgaaaccgtt acattataca
    26761 gcttcagcac tggcgctcgg actggcgtta atggggaatg cacaggcagt gacgaccatt
    26821 ccgttctggc attctatgga aggggaactg ggtaaagagg tggattctct ggcccaacgt
    26881 tttaacgccg aaaatccgga ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag
    26941 aatttaagcg cggggattgc cgcatttcgt accggcaacg ctccggctat tttgcaggtt
    27001 tatgaagttg gcaccgccac catgatggcg tcgaaagcca ttaaaccggt gtatgacgtg
    27061 tttaaagagg cagggattca gttcgatgag tcgcagtttg tgccgacggt ttccggttac
    27121 tactccgaca gcaaaaccgg ccacttactc tcccagccgt tcaacagctc gactcccgtt
    27181 ctctattaca acaaagacgc cttcaagaaa gcggggttag acccggaaca gccgccgaaa
    27241 acctggcagg atctggcgga ctattccgca aaactgaaag cctccggtat taagtgcggc
    27301 tacgccagcg gctggcaggg ctggatccaa ctggaaaact ttagcgcctg gaacggtctg
    27361 ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg cggtgctgga gttcaacaag
    27421 ccggagcagg tgaaacacat cgccatgctc gaggagatga acaagaaggg cgacttcagc
    27481 tacgtcggtc gtaaggatga atccaccgag aagttctata acggtgattg cgcgatgacc
    27541 accgcctctt ccggttctct tgccaacatt cgcgagtacg ccaaatttaa ctacggcgta
    27601 ggtatgatgc cttacgacgc cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga
    27661 gccagcctgt gggtgatgca gggcaaagat aaagaaacgt ataccggcgt ggcgaagttt
    27721 ctcgacttcc tcgcgaagcc agaaaacgct gctgagtggc atcagaaaac cggttatctg
    27781 ccaattacca aagcggcgta tgacctgacc cgtgagcagg gcttttacga gaaaaaccta
    27841 ggtgcggata ccgcgacgcg tcagatgctg aacaagccgc cgttgccgtt caccaaaggg
    27901 cttcgtctgg gcaacatgcc gcagatccgc gtgattgtgg atgaagagct tgagagcgtg
    27961 tggaccggta agaagacgcc acagcaggcg ctggataccg cagttgagcg tgggaaccag
    28021 ttactgcgcc gctttgagaa atcgacgaag tcttaatcag tgtaatgccg gatgcgtttc
    28081 gcttatctga cctggcatcg cgtgtaggcc ggataagcga agcgcatccg gcacagttca
    28141 ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc tcgcgctggc tgccttatct
    28201 gctggtcgcg ccgcagctca tcatcaccgt tatctttttt atctggcctg cgggcgaagc
    28261 gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc tccagccagt ttgtcggtct
    28321 ggataacttc gtcacgctgt ttcatgacag ctattatctc gactccttct ggacgacgat
    28381 aaaattcagc acctttgtca ccgtcagcgg tttgctggta tcgctgttct ttgcggcgct
    28441 ggtggagtac atcgtgcgcg gcagtcgttt ctaccaaacc ttaatgttgc tgccgtatgc
    28501 cgtggctccc gccgttgccg ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct
    28561 gatcacccat tttctcgctg agttcggcta cgactggaac cacgcgcaaa atagcggtca
    28621 ggcgatgttt ctggtggtgt ttgcctcggt gtggaagcaa atcagctaca actttctgtt
    28681 cttctatgcc gcgctgcaat ccattccccg ttcgttgatc gaagccgcag ccatcgacgg
    28741 tgcagggccg attcgccgct tctttaagat tgcgctgccg cttatcgccc cggtgagttt
    28801 cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac accttcccgg tgatcgacgc
    28861 cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg atttataaga tctaccgcga
    28921 aggttttacc ggactggatc tggcttcgtc tgccgcacag tcggtggtgt tgatgttcct
    28981 cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg
    29041 attgagaacc gtccgtggct gacgatattc agccatacca tgctgatcct cgggatcgcg
    29101 gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga cgctggataa acaggccgtc
    29161 tacgccgcgc cgatgacgct catccccggc acgcatctgc tggaaaacat ccacaacatc
    29221 tgggtgaacg gggtaggcac aaatagcgcg ccgttctggc ggatgttgct taacagcttt
    29281 gtgatggcgt tcagcattac gctcggcaaa attactgtct cgatgctctc ggcatttgcc
    29341 attgtctggt ttcgttttcc gctacgtaac ctcttcttct ggatgatttt tatcaccctg
    29401 atgctgccgg ttgaagtacg tatcttcccg acggtggaag tcatcgccaa cctgaagatg
    29461 ctcgacagct acgccggttt aacgctgccg ctgatggcct cggcgaccgc cacgtttctg
    29521 tttcgccagt tctttatgac gctgccggat gaactggtgg aagcggcgcg gatcgacggt
    29581 gcgtcaccaa tgcgcttctt ttgcgacatc gtttttccgc tctccaaaac taatctggcg
    29641 gcgctgtttg tgatcacctt tatttacggc tggaaccagt atttgtggcc gttgttgatt
    29701 attaccgacg tggatctcgg caccaccgtg gcagggatca aagggatgat cgccactggc
    29761 gaaggcacca cggaatggaa ctcagtgatg gcggcgatgt tgttaaccct gatcccacca
    29821 gtagtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa
    29881 taagatggca ggactgaaat tacaggcagt aaccaaaagc tgggatggca aaacctaggc
    29941 cattaaaccg ctgacccttg atgtggcgga tggcgaattt atcgtgatgg tcgggccgcc
    30001 tggctgcggg aaatcgacgc tgctgcgtat ggttgccggg ctggagcggg tgaccgaagg
    30061 cgatatctgg atcaacgacc agcgcgtgac cgaaatggag ccgaaagatc gcgggattgc
    30121 gatggtgttc cagaattacg cgctttatcc gcatatgaac gtcgaagaaa acatggcgtg
    30181 ggggctgaaa attcgcggca tgggcaagca gcaaatagcc gagcgcgtta aagaagcggc
    30241 gcgcattctg gagctggacg gtctgctcaa acgtcgcccg cgcgagcttt ccggcggtca
    30301 gcgccagcgt gtggcgatgg gacgcgcgat tgtgcgcgat ccggcggtgt tcttgtttga
    30361 tgagccactc tctaacctcg atgccaagct gcgcgtacag atgcgtcttg aactgcaaca
    30421 gctgcaccgt cgcctgaaaa cgacttcact ctacgttact cacgatcagg ttgaggcgat
    30481 gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt gccgaacaga ttggcacgcc
    30541 ggtggaagtc tacgaaaagc ccgccagcct gtttgtggcg agttttatcg gcagcccggc
    30601 gatgaacctg ctgacaggcc gcatgaataa tgaaggcacg catttcgaac tggacggcgg
    30661 tatcgtgcta ccgctaaacg gtggctaccg tcagtatgcc gggcgtaaaa tgaccctcgg
    30721 cattcgcccg gaacatattg cgctaagctc gcaagcagaa gctggtggtg cgactggcgc
    30781 atcaggagcg cccgacggca ggcagcacgc tgtggctgca tctggcggaa aatcagctgc
    30841 atctttttga tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt
    30901 cgctcatcgt ggcggcggta agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg
    30961 ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat
    31021 cttcctgctc catgacgaca atctcgaacg caccagcaac ggctggggcg tcgcgggtga
    31081 actgaactgg caggatttac tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa
    31141 aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat
    31201 ggcgaatatc gaaatcaaac ccaccactgg cactggacca ttaacgggca aaatggtggc
    31261 gctggcggca cgccaactgt gggccggtat gacgccgccg ctgctgtcat cgtttgagat
    31321 tgatgcttta gaagctgcac aacaggcggc accggaactg ccgcgtggtt tattgcttga
    31381 tgagtggcgc gacgactggc gcgaactgac cgcgcggctg ggctgcgtct ctattcatct
    31441 caatcataag ttactcgata aagcgcgagt gatgcagttg aaagacgccg gattacggat
    31501 tctggtttat accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga
    31561 ttgcatctgt accgatgcga ttgacgtgat tggtccgaac tttacggccc aatagttttc
    31621 aacggaatgt caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt tctcctctcc
    31681 ggcagcatat gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg
    31741 ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc
    31801 aacacccgct gagaattatt gtttatctgg ttttctaaat gctgctgttg caactgagtt
    31861 tgcgttttca gttgctgatt cagcatccct ttttgctgga tttgctgagt ctgcatctgg
    31921 gtttgcatcc gctgctggct gggtatctga taccccggct ggttagggtt gttcagagta
    31981 ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagtcgtttc
    32041 atcgcgtatc ctcctctgaa gatatccttt aagtttactc gcttcccgac aaaacgatga
    32101 ttaattcaga gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac
    32161 gatgataaaa ccgacgtttt tacgccgggt ggccattgct gctctgctct caggaagttg
    32221 ttttagcgcc gccgccgcgc ctcctgcggc gcccgtctcg tatggtgtgg aggaagatgt
    32281 cttccacccg gtacgcgcga aacagggaat ggtagcgtct gtggacgcca ctgccactca
    32341 ggtgggggtg gatatcctca aggagggcgg gaatgccgtt gatgccgccg tggcggtggg
    32401 ctacgcgctg gcggtaacgc atccgcaggc agggaatctg ggcggtggtg gttttatgtt
    32461 aatccgctcg aaaaatggca ataccacggc tatcgatttc cgcgaaatgg cacccgccaa
    32521 agcgacccgc gatatgttcc tcgatgatca gggcaacccg gacagcaaaa aatcactcac
    32581 ttcgcatctg gcttccggca caccgggtac ggtagcaggt ttctcgctgg cgctggataa
    32641 atacggcacc atgccgctga acaaagtcgt gcagcccgcg tttaaactgg cacgcgatgg
    32701 ttttatcgtt aacgacgcgc tggctgacga tctcaaaacc tacggtagcg aagtgttgcc
    32761 gaatcacgaa aacagtaaag ctatcttctg gaaagagggc gagccgctga aaaagggcga
    32821 cacgctggtg caggcgaacc tggcaaagag cctggagatg attgctgaaa acggcccgga
    32881 cgaattctat aaaggcacga ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg
    32941 cttgatcact aaagaagatt tagcggccta taaagcggtc gaacgcactc cgataagcgg
    33001 cgattatcgc gggtatcagg tttactccat gccaccgcca tcctccggcg ggatccatat
    33061 cgtacaaatc ctcaatattc tggaaaactt cgatatgaag aaatacggct ttggcagcgc
    33121 cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc tacgccgacc gctcggaata
    33181 tcttggcgac ccggattttg tcaaagtccc gtggcaggca ctgaccaata aagcctatgc
    33241 caaatccatt gccgatcaaa ttgatatcaa caaagcgaag ccgtccagtg agattcgccc
    33301 tggcaagctt gcgccttatg agagtaatca aactacccat tactctgtag tggataaaga
    33361 cggtaacgcg gtggcggtga cctatacgct gaacaccacc ttcggtacgg gtattgtcgc
    33421 gggcgagagc ggtattctgc ttaataacca gatggatgat ttctccgcca aaccgggcgt
    33481 accgaacgtt tacgggctgg tgggcggtga tgccaacgcc gtcgggccga acaaacgccc
    33541 gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt aaaacctggc tggttaccgg
    33601 tagcccaggc ggtagccgga ttatcaccac ggtgctgcaa atggtggtga atagcatcga
    33661 ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc
    33721 ggacgagctg cgtgtcgaaa aagggtttag cccggatacg ctcaagctgc tggaagcaaa
    33781 aggtcagaaa gtggcgctga aagaggcgat gggcagcaca caaagcatta tggttgggcc
    33841 ggacggtgag ttgtacggcg catccgaccc gcgctcggtg gatgatttaa cggcggggta
    33901 ttaatatctt aagccctccc ggtaggtgag ggcttaaatg acaaacttag attgcaaatt
    33961 ctctatttca cccgcgccat ataatacgca tcgacatatt caccattacg caatgcgtac
    34021 ttcttaccgg tcccttcaat ctcaaagccg aattttttat agaccttaat tgctggcgcg
    34081 ttatcgacaa acacggttag ttcaatgcga tctacccgta accagttgtc gcacatttca
    34141 atcatctctc gcatcagggc gctggcgacg ccgcggttct
//
LOCUS       DADLSE010000032        33852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLSE010000032 DADLSE010000000
VERSION     DADLSE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.032"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JN692_001489"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="JN692_001489"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7263833.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHEIDELI
                     LPPLAEA"
     gene            973..1347
                     /locus_tag="JN692_001490"
     CDS             973..1347
                     /locus_tag="JN692_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7263834.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQMDKTVPAEGN"
     gene            1380..2103
                     /locus_tag="JN692_001491"
                     /pseudo
     CDS             1380..2103
                     /locus_tag="JN692_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(2307..3218)
                     /gene="cysM"
                     /locus_tag="JN692_001492"
     CDS             complement(2307..3218)
                     /gene="cysM"
                     /locus_tag="JN692_001492"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7263835.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMLELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(3352..4449)
                     /gene="cysA"
                     /locus_tag="JN692_001493"
     CDS             complement(3352..4449)
                     /gene="cysA"
                     /locus_tag="JN692_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7263836.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(4439..5314)
                     /gene="cysW"
                     /locus_tag="JN692_001494"
     CDS             complement(4439..5314)
                     /gene="cysW"
                     /locus_tag="JN692_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7263837.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(5314..6147)
                     /gene="cysT"
                     /locus_tag="JN692_001495"
     CDS             complement(5314..6147)
                     /gene="cysT"
                     /locus_tag="JN692_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7263838.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYIWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGLEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(6147..7163)
                     /locus_tag="JN692_001496"
     CDS             complement(6147..7163)
                     /locus_tag="JN692_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7263839.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(7467..8258)
                     /gene="ucpA"
                     /locus_tag="JN692_001497"
     CDS             complement(7467..8258)
                     /gene="ucpA"
                     /locus_tag="JN692_001497"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7263840.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(8387..9244)
                     /gene="murR"
                     /locus_tag="JN692_001498"
     CDS             complement(8387..9244)
                     /gene="murR"
                     /locus_tag="JN692_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7263841.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            9408..10304
                     /gene="murQ"
                     /locus_tag="JN692_001499"
     CDS             9408..10304
                     /gene="murQ"
                     /locus_tag="JN692_001499"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7263842.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTELMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            10308..11732
                     /gene="murP"
                     /locus_tag="JN692_001500"
     CDS             10308..11732
                     /gene="murP"
                     /locus_tag="JN692_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7263843.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(11912..12811)
                     /gene="yfeX"
                     /locus_tag="JN692_001501"
     CDS             complement(11912..12811)
                     /gene="yfeX"
                     /locus_tag="JN692_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7263844.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVTVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12907..13482)
                     /locus_tag="JN692_001502"
     CDS             complement(12907..13482)
                     /locus_tag="JN692_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7263845.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13543..13992)
                     /locus_tag="JN692_001503"
     CDS             complement(13543..13992)
                     /locus_tag="JN692_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7263846.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(13979..14404)
                     /locus_tag="JN692_001504"
     CDS             complement(13979..14404)
                     /locus_tag="JN692_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7263847.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14618..15487
                     /gene="amiA"
                     /locus_tag="JN692_001505"
     CDS             14618..15487
                     /gene="amiA"
                     /locus_tag="JN692_001505"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7263848.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVINYF
                     HWFDNQKAHSKKR"
     gene            15491..16390
                     /gene="hemF"
                     /locus_tag="JN692_001506"
     CDS             15491..16390
                     /gene="hemF"
                     /locus_tag="JN692_001506"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7263849.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16396..17448)
                     /gene="eutR"
                     /locus_tag="JN692_001507"
     CDS             complement(16396..17448)
                     /gene="eutR"
                     /locus_tag="JN692_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7263850.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17494..17993)
                     /gene="eutK"
                     /locus_tag="JN692_001508"
                     /pseudo
     CDS             complement(17494..17993)
                     /gene="eutK"
                     /locus_tag="JN692_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
     gene            complement(18006..18665)
                     /gene="eutL"
                     /locus_tag="JN692_001509"
     CDS             complement(18006..18665)
                     /gene="eutL"
                     /locus_tag="JN692_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7263851.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPQHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18675..19562)
                     /locus_tag="JN692_001510"
     CDS             complement(18675..19562)
                     /locus_tag="JN692_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7263852.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19583..20944)
                     /locus_tag="JN692_001511"
     CDS             complement(19583..20944)
                     /locus_tag="JN692_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7263853.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSSVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            21132..22327
                     /locus_tag="JN692_001512"
                     /pseudo
     CDS             21132..22327
                     /locus_tag="JN692_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416937.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            22573..23160
                     /locus_tag="JN692_001513"
     CDS             22573..23160
                     /locus_tag="JN692_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263854.1"
                     /translation="MNEIKKPGKAATSSGRYEKQAKGEFLMSDMLAVEQETNNDVRQY
                     LNKINELRNKAPKNEETKHEEHTPDHYEAKQQEQAWRGNLRYLDTLNRLDEVLPRKLY
                     ERWEKEHTVNDEAVLRALCYFAGTGKNSQLGWCRVGRGTIDKRARLSKNTVKKCLDRL
                     VHHFKLVERTEGYIPGSAERECNEYQLLFKPYNMK"
     gene            24610..24801
                     /locus_tag="JN692_001514"
     CDS             24610..24801
                     /locus_tag="JN692_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7263855.1"
                     /translation="MNDSELMTVNEVAALFGVTRRTIFRWMNKIKGWPVPVSPIDSRI
                     KFIRSEILEFYKNKGARHQ"
     gene            24941..25204
                     /locus_tag="JN692_001515"
     CDS             24941..25204
                     /locus_tag="JN692_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263856.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFQEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKARLLFELSRRFGVSISTFK"
     gene            25222..25764
                     /locus_tag="JN692_001516"
     CDS             25222..25764
                     /locus_tag="JN692_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263857.1"
                     /translation="MDAFDVLTNKGDELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIEIIKPQIPQIVADAIKAAGLEKRIAGLKTNVEKMEMHKQSGAPTPDEYFSPD
                     AEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     AADPEAEQRKRVFNAIGIRY"
     gene            25861..26190
                     /locus_tag="JN692_001517"
     CDS             25861..26190
                     /locus_tag="JN692_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263858.1"
                     /translation="MFGNYISTSPEKIIMLALRIMQGIAKTLAEHVLDLKHSPLSKQA
                     MKRQTLRLWAEYSLGTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGID
                     INDGTGD"
     gene            26229..26435
                     /locus_tag="JN692_001518"
     CDS             26229..26435
                     /locus_tag="JN692_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263859.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            26894..27232
                     /locus_tag="JN692_001519"
     CDS             26894..27232
                     /locus_tag="JN692_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7263860.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            27213..27491
                     /locus_tag="JN692_001520"
     CDS             27213..27491
                     /locus_tag="JN692_001520"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263861.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDEKTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            27484..28944
                     /locus_tag="JN692_001521"
     CDS             27484..28944
                     /locus_tag="JN692_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7263862.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            28966..29787
                     /gene="murI"
                     /locus_tag="JN692_001522"
     CDS             28966..29787
                     /gene="murI"
                     /locus_tag="JN692_001522"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7263863.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(29906..30600)
                     /locus_tag="JN692_001523"
     CDS             complement(join(29906..30354,30354..30600))
                     /locus_tag="JN692_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7263864.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(31124..32527)
                     /gene="eutA"
                     /locus_tag="JN692_001524"
     CDS             complement(31124..32527)
                     /gene="eutA"
                     /locus_tag="JN692_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7263865.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGVAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(32524..33750)
                     /gene="eutH"
                     /locus_tag="JN692_001525"
     CDS             complement(32524..33750)
                     /gene="eutH"
                     /locus_tag="JN692_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HAY7263866.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMR
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
BASE COUNT         8562 a         8828 c         8298 g         8164 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgatctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgcttccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctattac aaaacaagga
      961 taaggtaatt cagtgaagaa aatcatttgt ctggccatta cgctgctaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttcccacc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggtagcttc gtcgtcatcg attaccggga agccgtatat cgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgttaattgc gcagatggat
     1321 aaaacggtgc ctgcggaagg aaattaatcc gctttggtaa ggcatttaca ggaggtaaca
     1381 tgaaaaaatg ctttatttgt cacgatgaaa aatcgaataa attttggtgg atagattacg
     1441 aaggggatag tttagctgtc aactatggca aggtaggtag tattggtaaa ttccatacaa
     1501 aagaattcga taatgaagaa cagtgtctga aagaagccag taaattgatt gccgcaaaaa
     1561 tgaaaaaagg atatcaagaa actccagact ttaacttcat ggatctctac tattttgatg
     1621 atgaagagat tgggttacat cttaaaacgt cacacccaaa cttccagtgc cattttactg
     1681 atccacttta tatgtgttgc tgggatgaag aatctccttt tggtagcgat gaaggtgctg
     1741 atgctctaaa cgaacttgaa aatagcctcc gttctggact gtgctgattt ccctcaaatg
     1801 ttaattgaaa ctatgtgggg tatgaaatac atcgctatgg acagtattct tgaagaggat
     1861 gtgcgtgcgc aattactagc cgatgaaatg agctctatcc agagcaatat gattacctac
     1921 gcgactgcat tcggtcagat caaagtcatg ggtaaaatct cccataaact taaaaagatg
     1981 ggactcaatg cactagcgcg tcatcagctt accgcaaaaa ttcttcaatg gggtgacggt
     2041 caggactcac caatacttca aaaaatgatt gatgacctta ctgcttttcc acacgaaaat
     2101 taaaaactgc atttgtcggc agcaacaact gccgacaaat cagattattg acaaagtgca
     2161 ctttgtttat gccggatgcg gcgtaaacgc ctgatccggc ataaaaatat tgctaattca
     2221 atatattgca gaaacattgt aggcctgata agcggagcgc atcaggcagt tttgcgtttg
     2281 tcatcagtct ccaatgctat taatccttaa atccctgccc cctgactaaa atgctcttcc
     2341 ccaaacactc cggtggagag gtagcgatcg ccacgatcgc agatgatcgc taccaccacc
     2401 gcaccagggt tagcttttgc cacccgcagt gctccagcaa ccgcgccgcc tgagctgacg
     2461 ccacagaata ttccttcccg caccgccagt tcgagcatgg tgttttccgc atcgcgctga
     2521 tgaatatcca gcacctcatc caccagcgaa gcgttgaaaa tccccggcag atattccgca
     2581 ggccagcggc gaatgcccgg aatactgctg ccttcttccg gttgcaggcc aacaatggtc
     2641 accggtttgg attgttcgcg cataaagcgt gaaacgccgg tgatagtgcc ggtcgtcccc
     2701 atgctggaga caaaatgagt gatgcgcccg ccggtttgct gccagatttc cggcccggtg
     2761 gtggtgtaat gcgcataagg gttatcggga ttattgaact gatcgagcag ctttccttcg
     2821 ccacgattcg ccatctccag cgccagatcg cgcgcacctt ccatgccctg ctctttggtg
     2881 acaagaatca gttccgcacc ataagcacgc atcgccgcac ggcgttcctg gctcatgttg
     2941 tcgggcatca gcaatttcat gcgatagcct ttcagcgcgg caatcattgc cagcgcaatg
     3001 ccggtgttac cactggtggc ttcgattaag acatcacccg gtttaatttc cccgcgcttt
     3061 tccgcctcga cgatcatcga aagtgccgca cgatctttca ccgaacctgc cgggttattg
     3121 ccttccagtt ttaaccacac ttcactgccg ttatccggcc ccattcgctg caacttcacc
     3181 agaggcgtat tgcctattgt ttgttctaat gtactcacga tctctatcca tacgtggtgt
     3241 tgcctgatgc gacgcttgcg cgtcttatca ggcctacagg ttacaaacct tgccattaaa
     3301 aagcccggat cgcgggaagc gcctccgggc gtttaacatt cactcaacct atcaggcgct
     3361 ttgtgcgaga gcaagttcct catcgcgggt ttcaatacgc tcgtcgccgt tatacagccg
     3421 cgcatgttgc agaccaacga ataaacgctc gccacgctgc ggggcatcgt cgccatgcat
     3481 cacgaccgtc agcggttcgt tgtaccaccc cagcggctgc accactaatt gggtgtagtg
     3541 acctttcggg ctggcttcca gtacctgtac cggcagcggc gaatcgagac tggtacggcg
     3601 gctgatatcc acttcccacg ggcgcaggaa gagatccacc ggaccctgat acgcaggtgt
     3661 gtagcccagc ggccagcgat gcgcgccaac atggaactgc ccgccgcgaa tggttccctg
     3721 caggcggttc acttcgccca taaattcgag tacaaaacgg gtcgctggtt cgcgccatac
     3781 ctgattcggc gcatcagcct gttcaatatt cccctgactc atcaccacta cacgatcggc
     3841 tacttcggtc gcctcttcct ggtcgtgggt cacaaacacg ctggtgaatt tcagctcttc
     3901 atgcagttga cgcagccagc gacgcagctc tttacgcacc tgcgcatcca gcgcgccaaa
     3961 cggttcatca agcagcagaa tttgcggttc tacagccagc gcgcgcgcca gcgccacacg
     4021 ctgtttctgg ccgccggaaa gctgcgccgg ataacgatcc gccagatgcg caagctggac
     4081 catttccagc aattttgtca ctttcgcttt gatggctgcg gcattcgggc gctcgcgacg
     4141 cggcagcacc gtcaggccaa aagtgatatt gtcgaatacc gtcatatggc ggaacagcgc
     4201 gtaatgctgg aacacgaaac cgactttacg atcgcgcgcg tgcaggcgac tcacgtcggt
     4261 gccgtggaag cgaatatgcc cgctggtttg atgctccagc ccggcgataa tccgcagcag
     4321 cgtggttttc ccggaaccgg acggccccag caacgcgacc atctgaccag aaggaatatc
     4381 cagtgagata tcgttcagca cctgggtgcg accaaaagac ttcttgatat tggcaatctc
     4441 aatgctcatg atgctcctcc tgctgcgcgc gtttttcctg attctccagg cgccactgca
     4501 acatactctt taaaaacagg gtgataatcg ccatcagcgt caacagcgcg gcggcggtaa
     4561 aggagccgac ggtgttgtag tcctgctcca acaattcaat ctgtaacggc agcgaaagtg
     4621 tttcgccgcg aatcgagccg gaaaccaccg acactgcacc aaactcgcca atcgcgcggg
     4681 cgttggtcag caccacgccg taaagcagcg cccagcgaat attcggcaat gtgacgcgac
     4741 ggaacatctg ccagccggac gcgccaagca aaatcgccgc ttcgtcttcc tggctgccct
     4801 ggcttaacat caccggcacc agctcgcgca ccacaaacgg acacgtcaca aagatggtga
     4861 ccagcaccat tccgggccag gagaacataa tttgcagatt atgttcatcc agccagccgc
     4921 cgagcgggcc gttggagccg tagaacagca gatacaccag accggcaacc accggcgata
     4981 cggcaaacgg aatgtccagt agcgtcagca gtaactggcg tcccgggaag ttaaagcgcg
     5041 tcaccagcca ggccagcaga atgccgaaca ccaggtttac cggtacggca atcagcgcga
     5101 tcatcaccgt cagccagatg gcgtgcagca tgtccggatc ggccagattc tgtaaaaccg
     5161 gcatcagccc cttgctgaat gcctgcacga agatgtaaat catcggcacc agcaggatga
     5221 acgccgaaac cagcatcccg atgccaatca gaaaccattt gccccagtta atcgggcgcg
     5281 cgtcataacg cttcaattgg gtaacttccg ccattaatga cctaccacac gccgaccaaa
     5341 gcgactttgc agagtgttaa ttgagaacag cagcagcaga gatgccgcga ggatcaccga
     5401 agcaatcgcg ctcgctgccg ggtaatcaaa ctcctgtaag cgcacaaaaa tcatcagcga
     5461 cgtcacttcc gtcttccacg cgatatttcc ggcgataaaa atcaccgcgc caaattcacc
     5521 aagactacgg gtaaacgaca gcgccacgcc cgccaccagc gccggagaaa gctccggcag
     5581 caccactttg cagaaactct gccagcgcgt tgcaccaagc gtttccgccg cttcttcata
     5641 ttccaggcct aactcttcca gcaccggctg cacggtacgc accacaaacg gaatgctggt
     5701 aaaggccatc gccaccgcaa taccaagcca gatataggtg actttgatat caaacttcgc
     5761 cagccattca ccgtaaaaac cgtttacgga aaagagcgag gccagcgtta aaccggcgac
     5821 tgccgttggc agcgcaaagg gtaaatccat cagcgcatca agcagcgtgc ggcccggaaa
     5881 gcgataacgt gttaaaatcc acgccatcag cagaccgaaa acgccgttaa aaatcgatgc
     5941 cacaaacgcc gacagcagcg tcactttgta ggccgcgact acctgcgggt tggtgatcac
     6001 ctcccagtac tgcgcccagc tcatctcaga aagctgcatc accagcgcag agagcggcag
     6061 cagcaatatc aggcacacaa acagcagact ggtgccaagg cttaaagtaa agcccggcag
     6121 cacgcgtctt gaagaaactg caaacattac ttacgccccg ccgctaacag cttgtctaac
     6181 tcgccgccgc tggtgaagtg ggttttcatc acttccggcc atgagccaaa tttgtcttcc
     6241 acgcggaaca gttcggtctg cgggaattta tctttcagtt tgtccatgac ttccgggtta
     6301 ttcacacggt agtaatagtc ggtgatgatg gtctgcgcct gtgggctgta gagccagttc
     6361 aggtaggctt ttgcggcttt ttccgtaccg ttggcctgca catttttatc gacccacgcc
     6421 accgggaatt ccgccagaat attggttttc gggatcacca cttcaaagcc ctgtgcttca
     6481 tactgtttac ggatgttgtt cacttccgat tcaaagctaa tcagcacatc gcccaagcca
     6541 cgctcggcaa aagtggtggt tgcgccacga ccgccggtat cgaacacttc aacgtttttc
     6601 aggaactggg tcataaactg ttcggttttg gctttatcgc caccgtcagc tttatccgct
     6661 gcgccccatg ccgccagata ggtataacgc gcgttacccg acgttttcgg gttcgggaaa
     6721 atcagcttca cgtcggagcg caccaggtcg ttccagtcgt ggatattctt cgggttgccc
     6781 ttacgcacca ggaagcccat ggtggagtag aacggcgagc tattattcgg caggcgcgac
     6841 tgccagtcag ccgggatcag cttgcctttg tcgtgcagga tttgtacgtc ggtcacctgg
     6901 ttataagtga caacgtctgc ttttaagccc tgcaaaatcg ccagcgcctg ttttgatgac
     6961 ccggcatgag attgttttat cgtcagtttg tcgccgccgt tatcttttgc ccattgctgt
     7021 tcaaacggcg gattcagggc ggcaaacagc tcgcgggaga cgtcataaga actgttcagc
     7081 agttccgttg cctgtacatg gcccgccagc agcagagaag cgaccagcgc gagtgagttc
     7141 tttttcagta agttaacggc cattgcgcac ccttataaat ttaatgactt tctaatagcc
     7201 atcatattta taacggacgt gaaaggagta acggttttat ataccgtttg gtgatttgga
     7261 agttaaaaag ggaataagaa cttggttcaa caggcgtaat tgttgcaggc agtttggaca
     7321 cggacagcgc ggagaaaccg gagcgtacac atagtacgtg aggatttcga gcactgcccg
     7381 gggccaaaat gacaaatgaa atagcctggt gaacttagtt caagacgaaa tcctccccac
     7441 acatgcaggg aggaaacaga ggtgaatcag ataccgacgc taaccgtctc cggcagtgtg
     7501 ctgccgccat caatcacatt ctgtgtaccg gttaaatagc tggattcatc cgatgcgagg
     7561 aaggccgcca gttcgccgac ttccagcgga tcggcgaggc gacacagcgg gattgctttt
     7621 gccatttcag tcagcaccga ttctggatct tccgggttcg actggcgggc aatgctttcc
     7681 gccattggcg tgcggacgta tcccgggcag atggcgttaa cgcgaatacc ggattgcgcg
     7741 tactccaccg ccagcgattt agtcaggcca acaatcgccg ctttcgttaa ggcatacgcc
     7801 gtttcgccag gatcggccac catatcacca gtgactgaag acatcatcac aatgcgacca
     7861 tctttgcgcg caatcatctc cggcagcacc gccttcgtga cgttccatac gcctttaata
     7921 ttgatatcaa tatggaaatc gcgatcttca tcgctcatat cgaggaaact gcccagacga
     7981 caaacgcctg cgttattcac caggatatca atgcgccctt ctttttcctt cgcgcgtttg
     8041 atagctgcgg ctaccgacgc cgggtcacgc acatcggcga caaccgccgt acagcgatga
     8101 ccacgaccac acagttcgtc cgctagcttt tcgatctcag gggagatatc cagcaagatt
     8161 aggttcgcgc catgacgtgc aaaagttctg gcaattcctt cgccaattcc ctgcaatgcg
     8221 cccgtaatca gtgctgtctt gcccgtgagt ttacccattt taatgctctc cttgttaata
     8281 cagagcgtta cactccattc cctctgaaaa tacaggataa acaaaatatt gctgcggatc
     8341 agctcactaa ttcaggaaaa tctcaatcca gtcgctgctc tatgcatcat ttcaggcgtt
     8401 gtgttagctc actgctgcgc tgaatcattt tcaatgacgc cacatcattg agttgcacaa
     8461 gaccaacaaa cagtaaatcg gtaacagaat tttgcgccgt gcgggtagac atcgacgagc
     8521 tacgccattc ggtttcaccg gaaacggtat cgagcgtgaa atgcgccagc cgccgcagcg
     8581 gagagtcagc cagagaagta atggcaatca ccgttgcccc ctgctttcgt gccgcttctg
     8641 cgcacaagac aatctctttt ttactgccgc tgtaagagat agcgatttgt acatcgcctt
     8701 ttttcaacgc ttgggaaacg gtggcttgta cgtgcgtgtc ggcttcgcag gccacgcgat
     8761 agccaatttt catcagtttg aacgacagat cgcgccccac cagcgccgat ccgcccaggc
     8821 ccgttatctg gataaacggt gctttactga tgacctcaat gattttttgc agccgcgcgt
     8881 agtcgaacaa cgcacaggtt tgctccagcg ccagctcttt ttcgcgattc agtttgcggg
     8941 caatcacttc cagcgaatca tcactggtaa tcgaactgtg caggtgcagc gccgtggcat
     9001 tcgttttctc ccggctggca ctgtattcgc caattaacgc catacgcaat tcagtaaatc
     9061 cttgcgcgcc gagcttctgg gcaaatttca caatgctcga ctggctgata ccgagctgtt
     9121 tcgccatctg ccgtgaagag acggatttca gctcgctcac tctggcccgc agaaagtcag
     9181 caattttttg ctcgttcccg gtaaattcac tttccgcatt gcgtattttc gtcaggtaca
     9241 acatcttcca ctctcctggg ctggcagcaa aaattccgct taagttctta tgacgctctt
     9301 cacactctgc gagtgtagac tcaatgattc ctttaagtct gtcaattcgg gatatatgat
     9361 tccatacatc tgcaattatt tgatcgtaaa tagtaaggtc accaccgatg caactagaaa
     9421 agatgattac tgaaggctcg aacaccgcct cggctgaaat tgaccgcgta tcgacgctgg
     9481 aaatgtgccg gattatcaac gatgaagata aaaccgtacc gcttgccgtt gagcgcgtcc
     9541 tgccggatat cgccgcggcg atcgatgtta tccacgccca ggtcagcggc ggcggtcgtc
     9601 tgatttatct cggtgcggga acatccggtc gtctggggat tctggatgcc agcgaatgtc
     9661 cgcccaccta cggcgtgaaa ccgggtctgg tggttggttt gattgctgga ggcgaatatg
     9721 ccattcagca cgcggtggaa ggcgctgaag atagccggga aggtggtgtt aacgatctga
     9781 aaaatattaa tttaacggca caggatgtgg tggttggcat tgctgccagc ggtcgcacgc
     9841 cgtatgtgat tgccggactg gaatacgcac gccagctcgg ctgccgcaca gtgggaattt
     9901 cctgtaatcc ggggagcgcc gtttcaacca ccgctgagtt tgccattaca ccgattgtag
     9961 gtgccgaagt tgttaccggt tcttcgcgga tgaaagcagg tacagcgcag aaactggtgc
    10021 tcaatatgct ttccaccgag ctgatgatta aatccggcaa agtgttcggc aacctgatgg
    10081 tcgatgtggt cgccaccaac gaaaaactgc atgtgcgcca ggtcaatatc gttaaaaacg
    10141 ccaccggatg taatgccgaa caagcggaag cggcgttaat tgcctgcgag cgcaactgta
    10201 aaacggccat tgtgatggtg ctgaaaaatc tcgatgccgc agaagctaaa aaacgcctgg
    10261 atcaacacgg cggctttatt cgtcaggttt tagacaagga ataacccatg gccaaagaga
    10321 tcagcagtga acttctgaac accattctta cccgtgtcgg cggaccggga aatatcgcca
    10381 gttgtggtaa ctgtatgacg cgcctgcgtc tgggtgtaca tgacagttca ctggttgatc
    10441 ccaatatcaa aacgctggaa ggcgtgaagg gcgtcatttt gaccagcgac caggtgcagg
    10501 tcgtttttgg gcctggtaaa gcgcatcgtg ccgcaaaagc gatgagcgag ctgctgggcg
    10561 aggcaccggt acaggatgcc gcagaaatcg ccgcccagaa caaacgtcag ttaaaagcca
    10621 aacaaacctc cggcgtgcaa caatttctcg ccaaattcgc caccatcttc acgccgctga
    10681 tccccggttt tattgccgcc ggtctgctgc tggggatagc gacgttaatt gccacggtga
    10741 tgcacgttcc ggcagatgct cagggcacgc tacccgacgc gctgaatttt atgaaggtgt
    10801 tcagcaaagg tttgttcacc ttcctggtga ttctggtggg ctataacgcc gcccaggcat
    10861 ttggcggcac gggcgtcaat ggcgcaatta ttgccgcact ctttttgctc ggttataacc
    10921 ccgctgcaac caccggttac tacgccggtt ttcacgattt ctttggtctg cccatcgatc
    10981 cgcgcggcaa tattatcggc gtgctgattg ccgcgtgggc ctgcgcccgc attgaaggca
    11041 tggtgcgccg ctttatgccg gacgatctcg acatgctgct gacctcgtta atcaccctgc
    11101 tgatcaccgc tacgcttgcg tacctgatca ttatgccgct gggtggctgg ttattcgaag
    11161 gtatgtcatg gctgtttatg cacctgaaca gtaatccgtt cggttgtgcg gttttagccg
    11221 ggctgttcct gatcgccgtg gtatttggcg tgcatcaggg ctttattcct gtttacctcg
    11281 cgttaatgga cagccaggga ttcaacagct tatttcccat cctttcaatg gcaggcgcgg
    11341 gccaggtggg cgcggcgctg gcactctact ggcgggcgca accgcacagt gcgctacgca
    11401 gtcaggtacg cggggcgatt attcccggcc tgctgggcgt tggtgaaccg ctgatttacg
    11461 gtgtcaccct gccccgcatg aagccgtttg ttaccgcctg tttaggcggc gcggcgggcg
    11521 gtttgtttat cggattgata gcctggtggg gtctgccgat gggcttaaac agcgcctttg
    11581 ggccgtctgg tctggtggcg ctgccgctga tgacttccgc acaaggcatc cttccggcaa
    11641 tggcggttta tgctggcggg attctggtgg catgggtttg cgggtttatt ttcaccacgc
    11701 tctttggctg ccgtaacgtc aatctggact gatattatga aacggacaat gctctatctt
    11761 tctctgctgg ctgttagttg tagcgtcagc gccgcaaaat accctgttct gacagaaggt
    11821 agttgatcaa gtatatgcgg cgttaaagca gtaataaaaa ggccagtcag taatgttact
    11881 gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg
    11941 gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgtttgc
    12001 catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt
    12061 aggcgcagaa gtacagaccg tgagtgccac tggcggtgcc gtacggcagg ctctggcgaa
    12121 caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtgaggtga gaggtttccg
    12181 gacgttcgtc gccgtcaatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct
    12241 gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg ctcccaacgc tgcacaaaca
    12301 catagctgcc gccggcatcc acgccgtctt tgataaccgt cacttcgcgg cgcgtctctt
    12361 cacccgccgg gttttctgta ccgtcaacaa agccgctcag gtcacgctct tccacccaac
    12421 ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct
    12481 gagcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact
    12541 gggtcgtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa
    12601 cgccgccgct cagagcgcgc caggtgttgt taccaaaggc aaccaccgca ccaagatgcg
    12661 cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac
    12721 gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt
    12781 ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataag aatgccacgc
    12841 aatccgtggc attgttgagc gctattttac gtgttttttc gcgacggcgg tttgttcaga
    12901 cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga
    12961 aggcattaac ccttcaggac cacgccattc cccgctgaac tggtagctaa tatgttgact
    13021 gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc
    13081 aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg
    13141 aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat
    13201 cgccacgttg tcgcctttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt
    13261 cattccgctg cgcaggcgat aatcgccatc aagcgcatca gcaatggctt gttcttgcag
    13321 tggtgtggac gccgttaatt cacccacgcc ctgctcgctc actttggtgg acgatccaaa
    13381 ccagttccac gggttagcgg cagaccagtt aactgacgac atcgtggaac agccggtcag
    13441 catcaacggc atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat
    13501 tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt tttcttcatt
    13561 aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac
    13621 taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca
    13681 gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agcacatgcc aggtgcgagg
    13741 aaacgaagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatac cgggaatcag
    13801 ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc
    13861 gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag
    13921 ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca taatggacag gatggaactc
    13981 agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc
    14041 ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacgttg atctgaattt
    14101 tcgggcagcc gcgagcaatc agctttttct ccagccggtt aagcaacgca ttggcaattc
    14161 cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat
    14221 aaccgcccat caccgttccg accacttcac cgtttacctc agcgaccaaa aacaaactga
    14281 cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt
    14341 cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct
    14401 ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat
    14461 tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt
    14521 gaaatgaaaa gtaaaacaat tctcaacagc aaaccgtcgt aacggattac gcgatacgat
    14581 ataacatctg gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa
    14641 aacactcact tcgcgccgcc aggtgctgaa agccggttta gctgccctga cgttgtcagg
    14701 aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc
    14761 gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcggaat
    14821 tgataccgga gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc
    14881 taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg
    14941 cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct
    15001 gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt
    15061 tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa
    15121 ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat tgcagcaagt
    15181 actgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgcttg gctcgcatat
    15241 tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt
    15301 tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa
    15361 cccggaagaa gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat
    15421 tgctgaaggc gtgatcaatt atttccactg gttcgacaac cagaaagcac attcgaaaaa
    15481 gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga
    15541 tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagttggca
    15601 gcgcgaagct ggcggcggcg ggcgcagtcg ggtgttgcgt aatggtggtg ttttcgaaca
    15661 ggcgggcgtc aacttttctc acgtccacgg tgaggcgatg cctgcttccg ccaccgctca
    15721 tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc
    15781 gcataacccg tatgttccca ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc
    15841 gggtgcagag cccgtttggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt
    15901 tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc catttggtga
    15961 agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa
    16021 cgaacagcgc ggcattggcg ggctgttctt tgatgatctg aacacgccag atttcgacca
    16081 ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt
    16141 agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg
    16201 cggtcgttat gtcgagttca atctggtctg ggatcgcggc acgctgtttg gcctgcaaac
    16261 tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga
    16321 ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga
    16381 ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca acgacggctt
    16441 ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg
    16501 catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac
    16561 ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa
    16621 cgcgttttgt agcgtgcggc gactaacatg cagttgatta cacaaatcca gcaccgtcac
    16681 cggttcggac atgttttcca gcacatattc acgggcgcgg gaaagcaacc gacggtaact
    16741 ctgatgactg atgctttccg ccgtcaccat tggttgcgct tcttccagca tcgcccccat
    16801 cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg
    16861 gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg
    16921 ctctttcact tccagcgccg actggctacg cagcatatgt aatacccgat ccggattgtg
    16981 caaaaagtta gcctgacggg tgatgacatc ttcagacagc actacgccca ggatcgtata
    17041 atcgtccggc gtacttaatt caaactcggt tccaccgggg cgcgtcgcta tttctgcgct
    17101 tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca
    17161 gaacgagttc ggccagacca ggcacgactg gcgcagcgcc agaccggtgt attcacgaaa
    17221 aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc ccggatgcag
    17281 ctgatcgtaa atctgctgcc aggcggtaat cgtcaaagca tgttcataga catccgttgt
    17341 ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa
    17401 gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc
    17461 gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact
    17521 actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg agcgcatttc tggcttttcc
    17581 agcggccagc caaagtgggc ggccacttct cctgccgtca ttccctgaca tactgatgtt
    17641 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    17701 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    17761 cgaccgatct ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    17821 tccagcgccg cacgacacgc cgtcagatcg ccttccacca ccagcgttaa gcgaccaggg
    17881 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    17941 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    18001 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    18061 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    18121 tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa cgcggcatca
    18181 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    18241 atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa ccacatgcgc caggaaggcg
    18301 gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc
    18361 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    18421 ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact
    18481 tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc gtcagaatcg
    18541 gcagaaatca gcccgaggct acgaatatgc tgcggcaatt ttagttcacg cgcaaattca
    18601 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    18661 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    18721 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    18781 ttagagatac aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    18841 tagcaggaga ggctttctga ctgccctaac cccggacgtt cccccaccag caggatcacc
    18901 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca
    18961 aagaatggcg tgccgacttt cagcccggcc tgtttcaggc ccgccatcag cggcggcagg
    19021 atctcttcgt agttgacggt gatggcatca gttgacaggc catcagaaat aacgacctgt
    19081 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    19141 cggcccatat ccgggcgagt caggtagaga tttttgtcgc taatttcaga gcgtacttcc
    19201 agcaagccct gcgctttcac ccactcttcc ggcacttctt tcagtacggt atctttcgaa
    19261 cgagagtgat cggccaggaa acgcagcagc gcctgagtac gtggacgcgg accggcacga
    19321 ccggtacaaa cgcgggccac ggtgctgcgg cgcagttctg ttaatacgtc tgcgcgatgc
    19381 gggttttcaa caccaatcca cgcttttgct tcagcggaac ctaaatccag cgcacagctt
    19441 tccgaggtca ccggtgccgc acagttggtg gtggcgcact ttgcttctga cggcgcgggg
    19501 gccgcttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    19561 atgatgtgtt atctccgcgt catcagaaga acagtgacgg atcgcccgcc cgtttggtca
    19621 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactct ggtgacgggc
    19681 gcaggttgag taactgacgc acagtggcgg tatcgtggaa tgcggtggtc tggtagttga
    19741 gcatgatgtc atcacccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    19801 ggatcatcag gttttcgttg aggttctggt cagagtcagc gtggttggtg tagcagcagt
    19861 cacagcccat agagatgccg ctcagcttgc ccataaagtg atcttctaag ccagcacgga
    19921 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga
    19981 taaacggatc gtaatgacgc gccagcccat agttacgtgc ttccatcgtc acctggtcag
    20041 cgccgaagtt agcgccagcg gatagcgcag agccttgtcc ggtttcgaag tagaggcagt
    20101 tttccccggc gatacgattg aactctgcgc ccactgcgcg cgcttcgtcg agcatcgcca
    20161 gttccacgcc aaactctttc agcccttttt cgctgccaca gatactctgg aaaatcagtc
    20221 cgccaggcgc gccgcgacga atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc
    20281 cctgagttgg gatgttgaat ttgtcgatca cgccataaat ggtatccagc acgctgctta
    20341 agttttccac gtcgtcagtt accgggttaa cgccgatcac cgcatcgccc accccgaagg
    20401 aaagcccttc atagatttgc gcagcgatac tttgcacgtc gtcacgggta tcgttcggct
    20461 gcaaacgggc gctaaaggtg cccggaatac cgatggtggt attggccttt ttgattaccg
    20521 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcga
    20581 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc
    20641 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    20701 cgttaacgtc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    20761 tattgcggat gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    20821 gtgagcttgc cgctgcaacg cccgccagca catcccccga acgcagttcg ttggctttag
    20881 ccagcacctc ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt
    20941 tcataagtcg ttccctcagg aagaagtgat gagtaacatt tcttccagca ttcgaacaat
    21001 tctatttcat cgttagccct tgtcctgtaa ggtgttaccc ttaatacgcc cgttacttat
    21061 tctgccggac acaaccgaac gcgccggata catgagcggg gttatcgagt cgttagatcg
    21121 aggtatccgg tttgagcaag acaccactga cagcaaaggc catagaggcc gcccagccgc
    21181 aggacaagcc ctacaaactc actgattcaa tgacgcctgg ccttttcttg ctggtacatc
    21241 ccaacggcag caaatactgg cgattccggt attggataga caagaaggag cggttacagg
    21301 ctattggggt gtatccgctg attagcctca aggaagcccg caaacgcgcc acagagagcc
    21361 gtttactgat agcccaggga attgacccaa tggatttggc acgcaaggag aaagccattg
    21421 atgctcttaa tgctatcgcc agttttaaag ctgtagcaga agactggatg gctaccaagg
    21481 tgagcggatg gagtgagagc tatacaaagc aggtcagatc ggcactagag aaagatgttt
    21541 accccgtgct aggtaagcgg tcgatagtgg atataacggc ccgtgacgtg ctggcgttac
    21601 tacagaaaaa ggagcgcacc gcaccggaac aagccaggaa attacgccag cgtatcgggg
    21661 agatcttcaa atttgccgtt atcactgaac tggttaaccg gaatccggtt gcagatctgg
    21721 atacggcact gaaagccaga cgcccaggcc ataacgcgtg gataccgatt aatgaaattc
    21781 cggccttcta caaagccctt gagagagctg ggagcgttca gattcagacg gcaatacttt
    21841 tgctttgagg acggcagaat tgcgcctgat gcgctgggaa tgggtagatc tgcaatcggc
    21901 aacaatcaca ttgcccgctg aagtcatgaa ggcccgccga ccgcatgttg tcccgctatc
    21961 ccggcaggcg gtcgagctat tacaggacca gtttacccgc agcggataca gtgctttcgt
    22021 ctttcctggc cgattcatgg acaagccact ttcagccagt gccatcctta aagccctgga
    22081 gcgtatcgga tacaagtcga tagccacagg ccacggctgg cggaccacct tttcaacggc
    22141 gcttaatgag tccggcaggt actcaccaga ctggatcgaa atccaactgg cccacgttcc
    22201 gaaaggcgtg cgcggtgttt ataaccaggc ggcctacctg aagcaacgac gggccatgat
    22261 gcaggattat agcgactgta ttgattcaat actggctggt gacggcaatc cgcttgagcc
    22321 ggaatgaata attaagcggg tttccgacct actccaccgg agcagactag ataaatactg
    22381 gaccccttca gaataaatat tcacctgccg gattcgtccg gcttttgttt ataaatcatc
    22441 caatcgtgaa attattagaa ttaaacttac atactagttt actagccaga ttaggctact
    22501 agtatataac atcaataaaa tcatgaggtt agtcaaaagt tgccgtcagg ggtcatgcgg
    22561 gcgtaacatt agatcaacga aataaaaaag cccggaaagg cggcaaccag ttccgggcgg
    22621 tatgagaaac aagctaaagg agaatttctc atgtctgata tgctagcagt agaacaagaa
    22681 acaaacaatg atgttcgcca atatttaaac aaaattaacg agttacggaa taaagcaccc
    22741 aaaaacgaag agactaaaca cgaagaacac acgccagatc attacgaggc aaaacagcag
    22801 gagcaagcct ggcgtggcaa cctgcgctac ctggacacat taaaccgact tgatgaggta
    22861 ttgcctcgca aactgtacga aaggtgggag aaagaacata cagtcaacga cgaggccgtg
    22921 ctacgcgccc tttgctactt tgctggtact ggcaaaaact cacagttggg atggtgcagg
    22981 gttggcaggg gaacaatcga caagcgagcc aggctatcaa aaaacacggt taaaaaatgc
    23041 ctcgatcgtc tggtccacca cttcaaactg gttgagcgta ctgaaggcta tatacccgga
    23101 tcggcagaaa gggaatgcaa cgagtaccag ctattattca agccgtacaa catgaaataa
    23161 cggaatcagg cgggcaatgt tcccgccttt tctttgcctt aattccatga attcatgggt
    23221 gggtcaaaag ttgccgttag ggtaggtcaa tttgtgcctt taggtgggtc aaaagttgcc
    23281 gagtaagaat ttcactatta agaattctag gcgacgggag ttttttagcc tcccggtcgg
    23341 tcacagagag tcgcctagga ttaaaacaga agaatttatt gccgcgcagc gctggatgaa
    23401 tgcaatcatc aataccgctt tggcgcgtca tccttcccag caagctggaa aggcccattc
    23461 actcacgcac aacctgcaaa ccagaccaac agaccatcat ggcgattcgt tcatgggtag
    23521 cgcgggtata tctcaaaggg tgatttcttc ttggtggacc ttcggaagac ttcgagcaat
    23581 tgccagctta aagacagctt agcagtgatg gttatcatca acagctttct gagctaccgc
    23641 acagacacac agcagcagta agcaaaccga agggaatgca aagcaaaagg attcgccaca
    23701 ctgcaagacc aatggttttt gtcacgtata cagaccaggc aagcaacagc agcaaagcag
    23761 cgtatagcca ggtgcagata gtaccatcac acaacgcaca gcaggctgag gtgaacactc
    23821 aagcaacttt atccccgcca ccgaccaccg caaactatcc tgaccgtgcc aaattcgaac
    23881 gtaatgccac ctggaacgcg atggagacac ataagggcta cgcttgctta gctgaaaact
    23941 tcacagcgtc acagcgctat ttagacgcat tagagtcgat gcgatttcat taaatttttt
    24001 agccatccag aaatcggcct gattctgagc cgattgccag ccggactact gaccagatcg
    24061 gaccaatcat ccaaggttgg atgaatgaaa tcacacgcag atcctgccag gctatttcat
    24121 cccaccacac agaacgctga gatctgatat taccgccgca ccattccacg ccagacgaat
    24181 caaaacaggc ttacccttcc aagtggatag ctaaaaatta aggctgtcag aatgcgttat
    24241 attgcgttta aacggcatcg aattttaatg tgcgcaatgg tgccagattg caggcaaaaa
    24301 aaatcccgcc ggagaaagcg gggaagtgaa atgaatcgtt gtagttatag atcacatttt
    24361 gagtaagggt aaccagcccc tgagtaatat tatccctgca ttacgtgaga tgcgcaatta
    24421 ttttttgcta attaaaagtt ttgtaaacat tacagaatat cccctttcac tatatggtaa
    24481 aagatgtgaa acagggacag gaggaatata atattaagta tcaacagtaa tgttggcctt
    24541 taatttcatt cagggcgcgg tgagggtttt ctccatattt tcctcaccgc tactttttga
    24601 ggtgagaaca tgaatgatag cgaattaatg acggttaacg aggtagcagc tttattcggc
    24661 gttacccgtc ggacaatatt ccggtggatg aacaagatta aaggctggcc cgtcccggtc
    24721 agcccgattg actcaagaat taagtttatc cgatctgaga ttttggaatt ctataaaaac
    24781 aaaggcgctc gccaccaatg aaaacagcta ctgagattat caaataacct attgatttaa
    24841 aaagctagct accgcaattt ctgaaggaat aggcagcatg aaaatcaaag caaacacaat
    24901 taaagaaata acatcaatca aagtaaaagg agcagatgcc atgcattcat tagacagtta
    24961 ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg aattccagga
    25021 aaaagtgatg cgttctgctg attatattgc ggacaagttc gtggaaacgg ttcggccact
    25081 tgttgatgaa gtagctgata aacttcagtc tgaaatgccg gaagacatgg aaggcactgc
    25141 aaaggcgcga ctcctttttg aattaagtcg tcgtttcggc gtgtcaataa gcacttttaa
    25201 ataatcggag gaggaaagac aatggacgca tttgatgtac tgacaaacaa aggcgatgaa
    25261 ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgtt aaataagcag
    25321 attaaagcga tccgtatggc ccagtttaaa ttaatcagca atgaagttat cgaaataatt
    25381 aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact ggagaaacgc
    25441 attgccgggc tgaaaacgaa cgtagagaaa atggagatgc acaaacagag cggtgctccg
    25501 actccggatg agtacttctc accggatgct gagtttatgg cgcaggttga ggaacgtctg
    25561 ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag ttcggcaggt
    25621 attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc cccgtctatg
    25681 ccggagccag aaccagaaat ggccgccgat cctgaagctg agcaacggaa acgcgtattc
    25741 aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac cccggctgac
    25801 ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag tctcgaaaca
    25861 atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc actacgcatc
    25921 atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca ttcgccactg
    25981 agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc gcttggaact
    26041 atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga ggaaatggag
    26101 ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca cagcgtggga
    26161 atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc gcgcgcgagg
    26221 agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc cagcgggcag
    26281 aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa gcagagttac
    26341 taaccaaata catcagtttc accaattact gcatctacga gtgttacaag gaagatgcag
    26401 aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac gtcatgttgt
    26461 tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca cttcagcatc
    26521 accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg taggaataga
    26581 ccaggttggc attgaaacac atctaccatg accataataa agtaattttt tgatatgttt
    26641 atggttttat attaattaaa atatatctaa aattgatagt tttaaaattg caaaatccag
    26701 attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga aaaataaatc
    26761 aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc tttcatttat
    26821 attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat tcagcgccat
    26881 acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt tctgtcaaac
    26941 atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa acatgattta
    27001 taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta tgctttccga
    27061 ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg gttcatctga
    27121 agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac gcgaagtaca
    27181 aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat aaattctgcc
    27241 gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg ataatgaatg
    27301 ctaaagaggt tttgaagcat gtcgatgaaa aaactattgg tgtcgttacg actttaggta
    27361 ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt aaagttaata
    27421 ttagttttta tatttacatc gtaattatat gtttctattt taaaaatatt cgagagtata
    27481 aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg gacactcgct
    27541 atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc agaatacggc
    27601 ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc tgttgccttt
    27661 gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg atggatagga
    27721 gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct acaagtcatc
    27781 ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat tgatgttgat
    27841 atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt cgtactattt
    27901 gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc gcaggtatct
    27961 aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat tcttggtttc
    28021 tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg tgatcttttc
    28081 cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct tgcttatgca
    28141 gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa aaaataccca
    28201 attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag tactttaggg
    28261 gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt ggttgcctac
    28321 gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc atttatgcta
    28381 gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac aggtgtatta
    28441 gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg tcatggaatg
    28501 gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt tacttttgtg
    28561 ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt tatgctctat
    28621 ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc tcaacctgat
    28681 acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg attcttaggg
    28741 tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac tggtagtcca
    28801 accagctatg ttctttattt gttagtatta gttgccctat ttttattaat tccaatatta
    28861 atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga accattttct
    28921 tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag gttaaatatg
    28981 tttagtgctg aatctccaat tggtgtaata gactcaggtg taggtggatt gacatcggta
    29041 aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga taatatgaat
    29101 gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa aatgctgact
    29161 tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat atcctcaact
    29221 ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat cttatccatc
    29281 attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg tattatcgct
    29341 accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt gaactcaaca
    29401 ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga aggtaatctt
    29461 aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat ttcccttcat
    29521 ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa tttttttgaa
    29581 gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat ctctattaaa
    29641 aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt gcaaataaat
    29701 actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat tacgaaacct
    29761 catacattca gtatcaggca gttttaaatt tatccggtgc accgtaaaac cccgtccttc
    29821 tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg gacaaaccca
    29881 gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata aacgctccga
    29941 tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga cccaaccgct
    30001 taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat attttgccgg
    30061 tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg ctggtcagca
    30121 tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga tcggttcggg
    30181 gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac ccgagccagt
    30241 gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag ataaacgcca
    30301 catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt ttaaagtccg
    30361 gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc cggcaccatt
    30421 aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat aagtataagt
    30481 gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt tagggttctg
    30541 accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa cgctggtcat
    30601 gagataacct caaaagcccg tattatacat cagattcaac taattagatg catcacccat
    30661 ccagcattat aaaacatact aacaataact aaattataat aatcacaact tacaatcaaa
    30721 taataaatgc ataagattaa caatgatttt ttctggttat atttatctaa aaaagcagaa
    30781 tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac taacaaactt
    30841 gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg tttttatttt
    30901 tcattttctc ttttcttctc tttccggttc tcttcttttc tggttttggt tttttccttt
    30961 ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc accacaaaag
    31021 catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga tataatccca
    31081 ctaacaccca gccaccaagc accatacctg ataactaccg tattcaggaa ggaaatgcga
    31141 gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca atgtcgatat
    31201 agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt tgtagctgtg
    31261 ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc agcaggggat
    31321 gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc agtaccgcgg
    31381 cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cgcgtcagtt ttgggatcaa
    31441 gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca tcaatcggga
    31501 tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt gtgctgccag
    31561 agagcgaaag ggtatgtgca cccgcgccaa tcaccgtggc gcgtacggtt tgcgccggaa
    31621 actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc agaagtgggc
    31681 caatatcggc aaaacagaac gggtcggcgg gctggtggcg ataacattcg cccacgcctc
    31741 cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt tgcatcaatg
    31801 cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc gccatccgcc
    31861 gggtaacctg cacaagctgc gcgccggtca gcgaacgggc gtcagtgcct gcaccgaagc
    31921 actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc tggctgtcgg
    31981 tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt ccggcatcga
    32041 acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga cacagccgtt
    32101 gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg aggtgcggcc
    32161 cggcgctggc gacgacaaaa tcgcccagcg actgagagag cgccatcacc gccgggcgag
    32221 cattgcgggt tttcgcgctt tcaccggtga tgatgatggc accagaatca acgctttccg
    32281 gcgcaacgcc cgcagcctga tattgctcta aaatcagcga tttcagttcc gcttctttta
    32341 aaccaccctg tttatcgaca ggggtaaaga acaccgggct ttgccagcta atttcgcgtt
    32401 taatgaattc gtagcgcggc acctgcgaca ccgccgcacg gttaaccagc tccaggtggg
    32461 agaaaatcac ctgggtggtg gtggtgccga tatcgatacc gacgctcaat agctggcgag
    32521 tgttcacgat tgtgcctccg cttcggtttt agtcgcggtc gcgtcttctt ttggcaccag
    32581 catcatcgcc acgccaatcg ccgttacgcc gccgatcaac ttgccgacaa tcatcgggaa
    32641 gatcatggcg ttcatgttgg cagcggcgaa gcctaagtgg tcgcccaggg cgaaagcagc
    32701 ggaaacggcg aaggcgcagt tgatgacttt gccgcgggta tccatctgcc tcatcatgcc
    32761 gaacatcggg atgttgttgg caagcgttgc caccatgccg gctgccgcga tgttgttcat
    32821 attcagcact ttaccgacgc tcatcagcgg tttttcaaac cagcgggtca gcagcagcac
    32881 cattggatac gcccctaaca gaacgcagga gatagaaccg ataacttcaa tggcgcgcat
    32941 cacttcaccg ggtttatcgc caggggccat aaagataggg tccagaccgg ggatcagttc
    33001 ccagccaagc agaaacttaa ctaccgcggc agcaagaccg agggtgatca atgcaacgag
    33061 gaatttggcg aagatctgga agccgttgat cattttttcc gggatgaatt tcagccccag
    33121 cgccaccagt accgcgacga taagcaccgg gatcatgttc atcaggatga gcgcaaaggt
    33181 gaattccacc ggctggccgt tgatctgtac accggagtac atagccacca ggccaccggc
    33241 gatacagcca attggaatgg tcacaatgcc cgccagcacg ccgagcgcca gataacgacg
    33301 atcagaaggt tcgataatgc cgagcgccac cggaatggaa aacacaatcg ttggccccat
    33361 catcgacccg agaattaacc cagagtatag ccacgctgct acgtcgccgc ccgccagctc
    33421 tttggcgagg aagaagccgc ccatatcgca cgccagcagt gttccggcga acattgacgg
    33481 gtttgcaccg agcatttcgt aaaccgggat aattaccggc ccgagtacgt gagccagtac
    33541 cggtgccagc gcggtcatac cgaccatcgc cagacccaga gcgcccattg ccataaagcc
    33601 ttcttcgaac tgaccgcctg atccttcgat acttttaccg aacttaccga ggaaacgagc
    33661 agaaccgccg aactgcgaca ggatcctgtc tacggcagct atcagcataa agaacatcat
    33721 gatgtacatg atgatttcgt taattcccat cgcctttact ccctgttagt tgttatttat
    33781 tggcggatgc ggcgtaaacg ccttatccgc cctacatgtg caatcccgaa ggccggatca
    33841 gacgcggcaa gc
//
LOCUS       DADLSE010000033        33432 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLSE010000033 DADLSE010000000
VERSION     DADLSE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.033"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN692_001526"
     CDS             1327..2106
                     /gene="ipaJ"
                     /locus_tag="JN692_001526"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7263867.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3052..3981)
                     /locus_tag="JN692_001527"
     CDS             complement(3052..3981)
                     /locus_tag="JN692_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7263868.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4198..4434)
                     /locus_tag="JN692_001528"
     CDS             complement(4198..4434)
                     /locus_tag="JN692_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7263869.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN692_001529"
     CDS             complement(4438..6339)
                     /gene="ipaA"
                     /locus_tag="JN692_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7263870.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSLGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQPDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN692_001530"
     CDS             complement(6348..7346)
                     /gene="ipaD"
                     /locus_tag="JN692_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7263871.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDTLSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKNALEELKKKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN692_001531"
     CDS             complement(7397..8488)
                     /gene="ipaC"
                     /locus_tag="JN692_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7263872.1"
                     /translation="MEIQNTKSTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIAHLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSKNSTASQIAGNIRA"
     gene            complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN692_001532"
     CDS             complement(8508..10250)
                     /gene="ipaB"
                     /locus_tag="JN692_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7263873.1"
                     /translation="MHNVSTTTTGLSLAKILTSTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KSFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN692_001533"
     CDS             complement(10256..10723)
                     /gene="ipgC"
                     /locus_tag="JN692_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7263874.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN692_001534"
     CDS             complement(10781..11407)
                     /gene="ipgB"
                     /locus_tag="JN692_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7263875.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN692_001535"
     CDS             complement(11423..11812)
                     /gene="ipgA"
                     /locus_tag="JN692_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7263876.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN692_001536"
     CDS             complement(11825..13309)
                     /gene="icsB"
                     /locus_tag="JN692_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7263877.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13623..15239
                     /locus_tag="JN692_001537"
     CDS             13623..15239
                     /locus_tag="JN692_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7263878.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNPKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTKYLNNKNWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESQHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRRELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVNVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKDIINKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHDTGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15249..15611
                     /gene="ipgE"
                     /locus_tag="JN692_001538"
     CDS             15249..15611
                     /gene="ipgE"
                     /locus_tag="JN692_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7263879.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15611..16069
                     /gene="ipgF"
                     /locus_tag="JN692_001539"
     CDS             15611..16069
                     /gene="ipgF"
                     /locus_tag="JN692_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7263880.1"
                     /translation="MSRFVFILLFFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESR
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16078..17193
                     /gene="mxiG"
                     /locus_tag="JN692_001540"
     CDS             16078..17193
                     /gene="mxiG"
                     /locus_tag="JN692_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7263881.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICEANKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17201..17452
                     /gene="mxiH"
                     /locus_tag="JN692_001541"
     CDS             17201..17452
                     /gene="mxiH"
                     /locus_tag="JN692_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7263882.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGELTLALDALAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17465..17758
                     /gene="mxiI"
                     /locus_tag="JN692_001542"
     CDS             17465..17758
                     /gene="mxiI"
                     /locus_tag="JN692_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7263883.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVGNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17764..18489
                     /locus_tag="JN692_001543"
     CDS             17764..18489
                     /locus_tag="JN692_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7263884.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVTLLVWAFKTGWFKRNKI"
     gene            18486..19013
                     /locus_tag="JN692_001544"
     CDS             18486..19013
                     /locus_tag="JN692_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7263885.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18955..19650
                     /locus_tag="JN692_001545"
     CDS             18955..19650
                     /locus_tag="JN692_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7263886.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIINTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19643..20050
                     /locus_tag="JN692_001546"
     CDS             19643..20050
                     /locus_tag="JN692_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7263887.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFDAGREADDSTW"
     gene            20034..20462
                     /gene="mxiM"
                     /locus_tag="JN692_001547"
     CDS             20034..20462
                     /gene="mxiM"
                     /locus_tag="JN692_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7263888.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20592..21224
                     /gene="mxiE"
                     /locus_tag="JN692_001548"
     CDS             20592..21224
                     /gene="mxiE"
                     /locus_tag="JN692_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7263889.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21211..22911
                     /gene="mxiD"
                     /locus_tag="JN692_001549"
     CDS             21211..22911
                     /gene="mxiD"
                     /locus_tag="JN692_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7263890.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22930..23997
                     /gene="mxiC"
                     /locus_tag="JN692_001550"
     CDS             22930..23997
                     /gene="mxiC"
                     /locus_tag="JN692_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7263891.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24010..26070
                     /gene="mxiA"
                     /locus_tag="JN692_001551"
                     /gene_synonym="virH"
     CDS             24010..26070
                     /gene="mxiA"
                     /locus_tag="JN692_001551"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7263892.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26083..26484
                     /gene="spaK"
                     /locus_tag="JN692_001552"
     CDS             26083..26484
                     /gene="spaK"
                     /locus_tag="JN692_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7263893.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26488..27780
                     /locus_tag="JN692_001553"
     CDS             26488..27780
                     /locus_tag="JN692_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7263894.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27873..28211
                     /gene="spaM"
                     /locus_tag="JN692_001554"
     CDS             27873..28211
                     /gene="spaM"
                     /locus_tag="JN692_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7263895.1"
                     /translation="MEALDKRIIYFQQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28198..29076
                     /gene="spa32"
                     /locus_tag="JN692_001555"
     CDS             28198..29076
                     /gene="spa32"
                     /locus_tag="JN692_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7263896.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29070..29951
                     /gene="spaO"
                     /locus_tag="JN692_001556"
     CDS             29070..29951
                     /gene="spaO"
                     /locus_tag="JN692_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7263897.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29956..30606
                     /gene="spaP"
                     /locus_tag="JN692_001557"
     CDS             29956..30606
                     /gene="spaP"
                     /locus_tag="JN692_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7263898.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30621..30881
                     /gene="spaQ"
                     /locus_tag="JN692_001558"
     CDS             30621..30881
                     /gene="spaQ"
                     /locus_tag="JN692_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7263899.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30881..31651
                     /gene="spaR"
                     /locus_tag="JN692_001559"
     CDS             30881..31651
                     /gene="spaR"
                     /locus_tag="JN692_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7263900.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31658..32686
                     /gene="spaS"
                     /locus_tag="JN692_001560"
     CDS             31658..32686
                     /gene="spaS"
                     /locus_tag="JN692_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7263901.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32699..32941
                     /locus_tag="JN692_001561"
     CDS             32699..32941
                     /locus_tag="JN692_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263902.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10438 a         4840 c         6688 g        11466 t
ORIGIN      
        1 ttaccccctg gatctgattt caggcgttgg gtgtggatca ctattgcacc gttcgtgaca
       61 cttgttgact gcctgtgcgg catctggtat caaggtggcg tgaagccggt tcaggttctc
      121 atgcggaacg ttggaaccta tatccccccg ataccaaagg cgaaatgtaa gtgacaaaat
      181 gcaatgaaca cgttctccgt taactgagga gcatggtgat aaataaaggt aaatgcagaa
      241 ggtgtagagt ctcgcagatt tctgacatca tagagcctaa cattagatgc ataacgctac
      301 agattgtaga atctctacag gtgaaatata cactggttgg cgtttatgtc gcaaaacatg
      361 tcattcaggt tcatctcacc aataaggata tgagtgaagt ggaggataag tgagtctgct
      421 gtcagagttt tctggtgtat gtcagtaact gcgaaccatg tctgcttgat atgggagctt
      481 atggaggctc tctgtacaag gttagggagc taacaaagca gggataaaaa gtccgcctga
      541 tgcaggcacg gttcgttaaa tcttttgcca ttggtaacaa aatggtgcga tgaatgttca
      601 ggctatcttt atcttgatgg tggttcagct gtttggtaaa gaaatcgctg taacaataga
      661 agaactgcag gcagtactgg tcccacatcg tttgctcagt caactggtga agttccgtag
      721 cgcgtaaatt aatgctttgc atgaaacgtt actggagtgg atgaaagaat acctgcatat
      781 atcattactg cgttggatta ccagtacaac agattgaatg aactggacat gttgatagac
      841 ggcattgaaa agcagcttat aagcatagtg cggcagagtt aattggagag cacttcgcct
      901 tctgagtatt aagtaagcat ggtggtacct gcggatacta tctattcact gtgctcggtc
      961 ggcagcctta ctgatcaagg agttggggga gttgataaca aagttgaaaa aacagctctc
     1021 aaccaaggtg gttattgtaa caatagcaaa taagctggtg tgacctgtat cacaataacc
     1081 gattatggac gtaaatacga caaaaatatg tcagtatcag gcggtattaa ttgctgatgc
     1141 tgatggacat taactcttaa taaaagggtg gatgctgaaa ggttgttatg gtgacggacg
     1201 tgatgacgac ggaaaagagt atgaggtttt ggatttaaag ttcgttcggg gaataaggac
     1261 ccggtcagct aattctcata gtagttcgca ttgctggatg caatactgtg ctgcaattac
     1321 tcgtcgatgt cggaacaacg gaagccttgc aaacggggat gtattcatac aggtgttatg
     1381 ttatatgggg ttttattgca gggggctata ccaagagaat atatgatatc tcatcagact
     1441 gatgtgaggg taaatgagaa tagagtaaac gaacaaggtt gttttttggc gaggaagcag
     1501 atgtatgaca attcttgtgg agctgccagt ttactctgtg ctgcaaagga attaggggtt
     1561 gataaaatac cacaatacaa agggtctatg tccgaaatga caaggaagag ttctcttgat
     1621 ttggataatc gttgtgagcg ggatttatac ttaataacta gtggtaatta taatccgaga
     1681 atacacaaag ataatatcgc ggatgccggt tattctatgc ctgataagat agtcatggct
     1741 acgaggcttc ttggattgaa tgcttatgtc gttgaagaaa gtaacatttt ttcgcaagta
     1801 attagtttta tttatcccga tgcaagggac ttattaattg gcatgggatg taatatagta
     1861 catcaacgag atgtgttatc ttctaaccaa agagttttgg aagctgttgc tgtatccttt
     1921 attggagttc ctgtcggcct ccactgggtg ttgtgtagac ctgatggaag ctatatggat
     1981 ccagctgtag gagaaaatta ttcttgtttt tcaactatgg aattaggtgc aagaagaagt
     2041 aattctaact ttataggata tactaaaata ggtatttcca tagttataac taatgaggct
     2101 ttgtaaattg tcgtttttta tctgtgaagg agcttgaata ttttggggcg gggggcataa
     2161 caaatgatat tactgagtac aaatttagct atattgggcg gattagttgt gtgggaagac
     2221 gaaatgtttc tcttgaaata agtgaactcc ctcaaggaat gattgtgtgc ttaaatattt
     2281 aagtgctggt aagtttgaga aggctgacat attttgcaga gcaaacagtg attgacttgg
     2341 atgatatttt tattttagtc actcgttgta attggagtat gctttatttt atttgaaaat
     2401 agatgtattt tatacccgca atttaaactg taagccctat gaggggtatg gatattataa
     2461 gttactcttt gactaagcat aacttttggg gttagaaaaa taaatctctt gttaataatt
     2521 aatatgattt taaaatcaaa cttgtttata attataaaat aatgtaagtc ggtcatttta
     2581 tgggaagatt aggagaataa aacagtaatt atatttgaga aatctgctaa tatattctta
     2641 aagctttttt gatactgtga acgaagtctg ttaatggtgg attttttgtt tatcaccatg
     2701 taaaaaaatt gagtagtaaa agcgttgact gctaatcctc gctgttttta ttgaaacgat
     2761 taaaaatacg ctgaatattt tcggccagtc actcatcttc acataaaatt ttgaccatca
     2821 tgccgcatcc cttagttgca caattcattt tcaatgatat gaatattact aatttcacca
     2881 gataatgttc tcgtggtcag cagggtatga gttttcaccg taattttaag gaaacacagt
     2941 gcgtggggat tgaataacag atagccgacg gaattacccc accggctgaa agtcaagttc
     3001 aacgaagatt tttattatct gaattgggca gtttacatca gtgttcgatg tttatgaaga
     3061 cgatagatgg cgagaaatta tatcccgaat agcttcatct atttctctct gaactgtttt
     3121 gtttatttta gataatgtaa atattagagt tctgtttgtt ttattctctt ttctttttat
     3181 gtaagtccgt ttgtctttag aaataaataa cgagtcaact tttagcgaag ggttttgctt
     3241 tctgtttttg cttttcttta tgatgtttaa aatttctttt ttatatatgt ccgggggcaa
     3301 atttgtatca aggtctttta tttcttcctt taatattggt agtgtagaac taagagattc
     3361 attagccttt tcaagtcctt tataataatt gaataatatc ttgtagtcat taaagttaag
     3421 ttctgacgcg attggaaata gagatattat ttcctgtgga acgcttgctg cctgaaaggc
     3481 acgagtgact ttcgcgcgag acagattctc ttttttggct atgtctttat aggacatccc
     3541 tgatactttc agaaaattaa gaccaatacc aagttctcgg atgctatgct cttttgctgt
     3601 ttgtatatcg tttgctagtt ttctggcatc aagagtagat atatattctt ttgaatatag
     3661 aacttcaaga tctgctcctg catatattgc agatgctctt ctacgagtgc catccagaat
     3721 ttcaattcta ccatcaatct cccttcctat tacagggaag aattgttgta gctttatagt
     3781 ttttatgata tcggctaatg attcttctgt tagggattct tgatcgcgta cattcgtttt
     3841 ttggttgacg aaggttaaat cttggatcct tttatgagga atcttggctt tgataaagga
     3901 aactttatta ccagaatgga gtgtaaactc tttcttttgg ccttccttta tacttaacaa
     3961 gtcgttgcac aaatccacca tatcacaccc tgtttattca tattgattta tattgacaaa
     4021 agttaaatgc agtgtgagca agattgtatg taactgttga tgatagtcaa cggtagtctt
     4081 aaaaattact attttaatag ctggattgat gcaatttatc tttattggat aaaagaataa
     4141 tattcttgtg gagctctgat gtgagagctc catagatgaa aaaatgctta tcaattacta
     4201 tattgatttt gtactatttt tgactacaga aataagatct ccaatagtaa taatgtgttc
     4261 taatgcagat tcatctatac gtagattgaa ctcaaaacta atcatatcca gcatttcaat
     4321 cagagaaaga gaatcggcag aaagatcttc aataaattta gtttcttcag atagtttgct
     4381 gtacgctata ccatagcaaa aggccactat tgataatatt ttttctttta tcatatttta
     4441 atccttattg atattcttta atacttttga tagggaagtg gttacatctt ttgctgcttt
     4501 atatatatta ttgttttcag ctgtaatatc attgttattt tttaaatcag atatatcatc
     4561 tgtaagtaat tctgcagagg tatcgtcaat ttttgacaat accttgctga gggcgctaga
     4621 tacttctttt gccttttcat agatcgcatg atttttatca attgtatctg cgttggtttc
     4681 tgttacgtag tttattgtat ttgcatctgt aacttttaat ttgttgttta ttctgtgttc
     4741 tgttgtggat tttgtaatgt cctttgattt ttcctgtaac tctcgttctt gtgttcctga
     4801 tttagttcca atgagagata taagattact tagggagttt gttacttttt ttgaagcttc
     4861 aaatatcgtt tcccgtgtta aatatgtgtc tccatttctt gataaaaaat caggctgaag
     4921 tacttttgaa tcagtgtttg agttgaattt attgatttgc gaagctactc tatgagaaag
     4981 agaagcgcta cttttagttg tatctgatga gttatcatta ctgcttgtca ctccttccgg
     5041 gtggagaggg ggggtggttg aagagttctg tatgttcaga ggcctgtgtc ccagagaaag
     5101 agagttattt gcagatcttt cttgactact cgtagttaac aggtctgaac ctattccatc
     5161 cgaattatct ggagtatggt tgaaaacctt ttctaaagct gaattatttg caaatgtctg
     5221 gacagtatct gatatagagt gtggtagaac ggaagttact ttttgaacta gtgctctttg
     5281 tggatttagt aatgagtttc cgttacggga taatagatcg tttgtctgag aagtaggaga
     5341 ctgtgcatca ttttctgggt tctcgtgata gctattgtca tatactctgt tgtcaaaaac
     5401 tctattatcg taagttctat tatcattatt tatatgatag tgaattacag gttgggatgt
     5461 tttctcattg gctccggggg ttggtccgcc atctggcata tcttcgggag gtgtagggag
     5521 gccatcttgg ataggatgcg aatcttttat atcttctaaa atatcattaa ggacttttct
     5581 caacagctca ggaaacacgg ctactgtatt taagtctctt gacagtttac ctataccaaa
     5641 tcctttacta tcaatagtat ttttacgttg attgcgcaaa ctatcaaagg caagttttgc
     5701 ctgtgcgtca actaaatcat ctagtttgag agatgaaaca gtttcattac ttaactttaa
     5761 ttcagcaaga atattattct tgataaatga tgatacttca gtattgagga gtttttgtcc
     5821 caatgataaa gcttcaccag atgtgtgttg ttgtgctaat agtgcaagtc tgtgctcaat
     5881 gttttgtcgt aattgatgga gtttcttatt ctttgccgac catggtccaa aggcagaatc
     5941 ttgggataat ttatctttta taattagttc cgaatactgt gcgacaattt tttgaagtcc
     6001 cttttcgttt tcaaaattaa gaccattttg atgtactaag gagtacaaaa catttgaaaa
     6061 ttcgagaatc tcgcttttgg catacgcatt cattgagttg gaataaatgc ttcttaacac
     6121 atcttctttc cctgaggaag aaaataaaaa atcaagacat ttctgattaa aagacgttgc
     6181 aaagttttct ttttcagaca ctgatgctgg tgtttttaga gaagtttgcg aactatggat
     6241 atcggatatt ttgcttttta actcgctgta ttctgttgat gatggtgaag ttgccttata
     6301 taagaatgtt ggcgcttgag tattatttac attatgcatg gcgcacctca gaaatggaga
     6361 aaaagtttat ctgtatctgt acatgagctt attgtactac tcaaaacctt tactaaatta
     6421 tcaaaaatac tattggcatt actgtatttt tgaactaaag tttgaagatt atttttcatt
     6481 gtttcatctt cggcagagaa tccggcattc catgcctgat attttgcatt atctagcaca
     6541 acctcgccat ttccacctag attatctaag ctttttaaca tattgtctat tggggtcatg
     6601 tttatattga caacatatcc cccgtttttt tcagatacct tgccgattgt tccacctaat
     6661 tctgtaagcc atttatttgc ttgttcctga ctaacagtat tatttgctgg atatagcggt
     6721 ttatctttat attttttctt gagttcttcc aatgcgtttt taagcgagtt gacttgtaat
     6781 ttcacggagt ttccgtcgtt acctccggga gagatccagc cggcaagact ggaaagaaca
     6841 gcgctaaaat cttgatacat ttgagtatat gaactaacgg catgttcata tactttcaga
     6901 tactgttcat taatatcatt gatggagttt gcaattttag cccacagttc tctatgagat
     6961 atcatttgat ctccatcaag ctcggcttct ttcggggctg aatgtaataa ttctcttgcg
     7021 tctttattaa ttggatattc gttcctggaa agagtatcca atagttgagc ggatttattt
     7081 acatccatgc taatctgaga tgaatgtaat gctatttctt ctagcgatgt tttagtcaac
     7141 gttttttgtg aaagctcttt ctttaatgcc tgatttgttg ttctgatatt atgaagggtg
     7201 tcgttgagca tagtaaggga acttacagga tgagaactgg tcgttgtttt tatatcagaa
     7261 ttaactgttt cggttgatga accgttggta ttgtttggac tgaatgatga ggtggaaata
     7321 ctattagtca gagttgttat attcatggtt atttccttac aggaagagcc atatattatt
     7381 atggctcttg gatatattaa gctcgaatgt taccagcaat ctgactggct gtcgaattct
     7441 ttgattgggt gatgctgtca attatattca ataatttttg tattaattga tttgtcgctt
     7501 gggatgcttc tttagatact tgggatgctt cctctgcttg tttactgctg gcctgactga
     7561 ttagttgttc ttcttcttca agagcagatg cataacgccc tcctgatgtg gatatattac
     7621 cagcaactgc tgatgtgtcc attgttgcac ggccaatgtt ttgtttttgc tgcgcagaaa
     7681 gggtattgag ctcgtaagtt cttctctggg tgtcaatttt atcctgcaaa gaaatatcgg
     7741 gagaactaag agaagtttta tgttcagttg acagggatat attatctggc gccagtttat
     7801 ttttacctaa aaattttgtg ctagagttag tttgtggtga ggtgatattt gtatctattt
     7861 gtttatttaa ccctaattta gaacctgcaa gctctttttc aagagattga gcagtggcaa
     7921 ggttctttct taaggctcct ttttggtcgc taatccctga atgcgttttt ttggcaccga
     7981 tacccgttat acctacttgt gtgactgctc cagtaatgct tgatgatagg gctgccaggc
     8041 cttgccgaac aatgttctct gcagctgatt ttgtagcatc gaacgcaatc aatgacaatt
     8101 gagagcccaa tttagtttct gcagtgcgga gagcagaaag tagaacggct acactaataa
     8161 ttaaagaaac agcattagaa gaaagactgg aaatatccag agtgttctct ggagtaaggg
     8221 tgtgactcat ctctgaaaga tctttcatct ttttgtcagt aaggtgagca atgatttctg
     8281 aatcatgctg aacctgctct gataacttta aaagttgatc atcatttaat gtggttgtta
     8341 atacgggatt tttaccgaca ttaagtggaa tatgcgctgc aatctgctga taattttgtg
     8401 atttttgtgt ttcggaagaa ctttgagttt gttttgtaga tatatctgta tataaaatct
     8461 gggttgattt tgtgttttga atttccataa cattctcctt atttgtatca agcagtagtt
     8521 tgttgcaaaa ttgcttttgc aacatcagtt ctattagcct gagagttgga cattgaggct
     8581 aatagatctg caattacttc ctgcaattgg ccgaattttt ctattgcttc actgatatat
     8641 tttgacagtt gttcaacttg atatttcgat aatgtcaggt ctgctagatt tgtcgacgcg
     8701 ctgttctgga aaacagcaga agcgacactt cctcccgctt gtgtggcaga gttaacactt
     8761 tctcctaata aaactgcttg gtttaaatgg gtggaaataa tttgtttgga tattacttca
     8821 tcacccgctg caccaagaaa tttatttaat ctggcaacag cattagtgat taaatcgtcc
     8881 agttgagaag aaaaactctt gagaaacttt ggtataaggt ctgtgagggt tttacctatt
     8941 attttgccaa tattctctgc aagttttgct gctgcctgtt taccaacagt ggctacgaga
     9001 acgactgctg caactaggac aagagcgcct gcgattgccc ccagaataga gccaatcatt
     9061 ttggcttttt tcgagtcgac gcccaagcct tcgagcattt ttgtaaatgc atctgaaagg
     9121 agtttgatta agggttcaat gactgctttc atgatcggat tcagggcttg ttccatgaag
     9181 gaattgccgg tcgctgcttg tactatagca tccgtaacca taagagctaa accaacagct
     9241 gccagtgcta gagaggctcc tccagaaaaa gctgctgcaa caacactaac gatagttaaa
     9301 agtgccccaa gtattttccc aacacaaccc attactctgt tgagttcttc tgctttacgt
     9361 acttcagcag catactcatc agattttctc tccatttcag tttttcttga ttcttggaga
     9421 gactggaata gagccagatc attttttaaa gattcttcat tattttttcc aactagttga
     9481 ataaaggttg ccatcaattg agtaacactg gcaagtccgg ttaatgattt ctgctgggtt
     9541 gatagctgtt cagcagatgc tgtgtttgaa aatgcagaaa aagagtctat ttctttttcg
     9601 agttgcattg atttatctgt aagtttgcta tgaattgaca gggttttctg ctcaatcaat
     9661 gtcctgtctt taactgctgc gtcttttttg atagtgagtt gtatttcttc ccggcttaat
     9721 tttttctttt ctggtgaatc tggggcgagt tcggataatc ttgtttgaat ttggttaatt
     9781 ttattttcta ggtcttttat tttagaatct gcgtttttta gtttattaat ttgtttttca
     9841 tagtctctgg ttagtccttc agtttcagat agaagagtat taattttatc ggagaattct
     9901 aggttttttt gctgtcttgc ctgttgctga gacttccaag cagtaatttt atttgttaat
     9961 gcagttaaag atttttcacc gagtatttga ataaggtttc caattaaaag cgttagttgg
    10021 gaacttgcat ttaatgactt tggtgcttta agttcaggga ttaatatatt tgaagttgag
    10081 tgtgcattag ttgtattaat atttttgtta gcagtaagat cagcaattgt aagagaaaat
    10141 aatttgttag ctgcatcatt tgcagcctgg atagtattgt ctccaagctc agtggaagtc
    10201 aatattttgg caagagacaa accagtggtt gtggtactta cattatgcat aataattact
    10261 ccttgatatc ctgaattgcg tccaagtatg attgtgcttt tatttttaat ttttcatcat
    10321 tgctgtgttg aattacgagt tcgaagcact ctttggcttt caagggggct ttcaaccgaa
    10381 gctggcattg tccagtatgg aataccggtg tatagtcatt ttttcctaat gcaaaagcga
    10441 cagcataaag gtctgctgct tgttggaact gttcttttat ctgataaata gctgcgagtc
    10501 ccataatgta gtctacattg taaaagtcgt atatacataa aaatctgaag aaaacttcag
    10561 cttcctctat tcttcctttg ttgtaaaagt cataagcata tgaataaatg tcatccatca
    10621 tatcatcagg aattgcatta atatctttca gtgtagcgcc agagttaatt gcatcaatta
    10681 ctgcagtaga gatgctttca ttttcagtga tatttaaaga cataaggtct cctttttgtc
    10741 tgctttactt taaaaagtat aagatttaat ataaacagat ttaatttgta ttgctttgac
    10801 ggtatacggc ctttagaacc tctgcgctta tttctatttg taaaactttt gttgattcat
    10861 taaatatttt atcgtagacg gaactggata ctagatttgt aagatccttc atttgtgtgt
    10921 taaacaaatc catgtcactg ctatagcgtt ttaaaatctt tgctctgagg ccagatgagg
    10981 caatctcgtt tagtgctgta tgactgatag atgtttgtgc tgatttttca tcaagtttta
    11041 tatccgggaa ttttttttca atagctgcaa atatgttttt tttgttatct gaggtcaaaa
    11101 caactccatt gtgtatggcc atttcatcta tacttctgtt tatcattgaa gagacataag
    11161 aggttcgctc ttggctcatc caacaaaata gttgattccc cgaattacta tcttttattt
    11221 tgtctatgac gttgtccctg attttattta tattcaccgg gcatccaaaa ttcttctgag
    11281 gaaagcgttg ttttaagtta aaaacagata atattttctt tacaagctta ttgtaactta
    11341 acgaattaaa tgatgggcga ggaatcgcga attcaacctg tggaagtatt ttgtttagaa
    11401 tttgcatgat accccctata tgttagttca cttctgaagt gatgtttgca atggttagtt
    11461 taatacaaaa taaaaattca tacaaagatt caatgagttc atcaggtgaa agatattttt
    11521 tgtagtgttt cttggtcaaa acacattgat gagtatcagg gagaagacag atagaagaac
    11581 cataatctct actcaaagag tagttaagga tgagtatgtg ttctggatta ataatatttt
    11641 ctgtaaaaag ttcatgttca ttaagaagtc cggtcaaaaa gatgtattca tcattatctg
    11701 ttatacaaac aggtatttgt tcttcgagca atataaaggc ttgattttta tcgttaaaat
    11761 caagctttgc acctgttatt tcataaagtt tatcatatag tttgcgacac atttctatgg
    11821 ccttctatat attagaatga gagttattca ataaaagctt attgatctgt aataagtcgt
    11881 gaatgtaaag tttggttcca agatctggcg atttaagaga attgtaataa tcaatagttt
    11941 ttattgactt ttcgataatt tcttcttttt tatctgttgc tccggttttt aattcgttta
    12001 atacacgaga catattgctg ggtgctgatt ttactaagta tgagtggcaa gcgttgatga
    12061 ccgcatcaaa gtcttctgaa gatacatttt cagtaggatg tttgtaaagt gaaattgata
    12121 aaggcttgaa tgtttttggg gtgtggataa ggttaatttt attgaaactt ttaagatagc
    12181 tacgtcgtaa ttgagtcaat ctggaaagat caaaatcaga ttcaatatac ttcattatct
    12241 cgttataaga ctgattgaga ctttcaatgc gttgcctaac tttatttgca tacgcgtata
    12301 cattgttagg tgtagcaacc aatttaacgt cagggagtgg aaagtatatt tcagaatttc
    12361 ctgctttaag aacattaagt gccataccag cacagttttc agatttactg attaatttat
    12421 aatttgccaa ttgtctgaaa gcatctgctt tcttttggca tataaatttt gacatgtttg
    12481 tttcatcaag gccaaaaagg ttatagctga ttttaccatc ctttgtttta ttatcacctg
    12541 ataatggaat gtatacttta tcagcactga ccccccaata accatagggg tctttttttt
    12601 gattagctct gggaaaaaat atttttttgt tgttattttg atcttgtttt gaatttaata
    12661 agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta
    12721 gtttgtcttg tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta
    12781 tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt
    12841 cctgtagtaa attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat
    12901 ttccccagca acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa
    12961 aacgctctgc ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg
    13021 aatttttgac tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg
    13081 tcttgaggaa aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat
    13141 ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct
    13201 ttaggttgaa tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag
    13261 gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gagaatcata ctttattaac
    13321 tctccattac gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt
    13381 aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata
    13441 tgtacctcgt gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc
    13501 tgatatatca ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt
    13561 tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgacg gaggataatt
    13621 aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata
    13681 aaggcgcaga agaaaccgga aacccaaaag gtgtaagcgt gatttcatat caaagagtta
    13741 aaaatggaga gagaaataag ggggtcgaag cgcttaatcg attatattta cagaaccaaa
    13801 caagcttaac agggaaaggc cttttgtttg cgcgtgacag agcagccgtt ttttacgagg
    13861 caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa caattagata
    13921 cctataaact tggtgaagtt gataaaagaa atattaatga gcttaataaa gtaataagtg
    13981 aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaatcg gaaataaaac
    14041 aaatatttac taaatattta aataataaga attgggaacc gatcaacaaa aatattaatt
    14101 atcatgggaa aaattatggt tttcaattaa cacccgcctc tcatatgaag atagggaata
    14161 aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga
    14221 gtcagcatat tgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt
    14281 tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa
    14341 gagctgttgc tgctcgtaat aaagctgagg agttagcaag cgcagcatta tatagcagac
    14401 gagaattatt atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt
    14461 ctcttctgac gccgaccagt ttaactgggg gagaggagag tatgttaaaa gatcaggtaa
    14521 aggcattaaa agggttaaac tctaaaaggg gggaaccaac gaaattatta attcggaata
    14581 gtgatggtct tctgcaagaa gtaaacgtta atctaaaagt ggtgacattt aactttggtg
    14641 taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatggtg
    14701 aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg
    14761 ctgcagaagc tatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc
    14821 agataaaaga tattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat
    14881 tgtcacaaag aatggctcta ttggcttata ctataggtgc tgtaccttgc tggaattgta
    14941 agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa
    15001 aacatgatac agggcagttt tctcaattaa atagtaaatt atcctcagaa gagaaaagat
    15061 tattttctac tattctaatg aatagtggta atatggaaat ccaagagatg aatacgggtg
    15121 tgcctggaaa taaagttatg aaaaaattac cgctatcttc gttagagctg tcttatgctg
    15181 aaagaatagg ggaccccaaa atatggaata tggtgaaagg gtattcgtca tttgtataag
    15241 aggaatatat ggaagattta gcagatgtta tttgccgagc cttgggtatc ccttcaattg
    15301 ataccgatga tcaagcaatc atgcttgatg atgatgtgtt aatatatatt gaaaaagaag
    15361 gagattcaat aaatttatta tgcccttttt gtgcattgcc tgaaaatata aatgatctta
    15421 tatatgcgtt gagtctaaat tactcagaaa agatatgttt agctactgat gatgaaggtg
    15481 gaagtctaat tgcgcgatta gatcttaccg ggataaatga attcgaggat atatatgtta
    15541 atacagaata ttatatttcg cgtgtgcgtt ggctgaagga tgaatttgcg cgaagaatga
    15601 aggggtatta atgtcccgtt tcgtatttat tttgctgttt tttatcccat atttagggag
    15661 agctgattgt tgggataagg ctggtgaaag gtacaacatt ccatcgagtt tgttaaaagc
    15721 gattgcggaa aaagaatcca gatttaataa atctgcagtc aatgttaata acaatggaag
    15781 taaagattat ggtataatgc aaataaatga ctttcattct aaaagactta gagaaatggg
    15841 atatactgag gaaatgctaa ttagtcatcc atgtctttct gtacattatg cagcaaagtt
    15901 attaaatgag tttatgctga tgtatggaag agggtgggag gctgttggtg cctataatgc
    15961 aggaacatct ccgaaaaaga aaaaagaaag attaaagtac gcagaggata tctataaacg
    16021 ctatttgcgg atagcagcag agagcaagca gaataatcga aggatataag aggatttatg
    16081 tctgaggcaa agaactcaaa tcttgcacca ttcaggttat tagtgaaact cacaaacggt
    16141 gtcggggatg agtttccgtt atattatggg aataacttaa ttgttctcgg acggacgata
    16201 gaaacacttg agtttggaaa cgataatttc ccagaaaata ttattccagt aacagatagt
    16261 aaatcggatg gtataattta tttggccata agcaaagata atatctgtca atttagtgat
    16321 gaaaaaggtg aacaaattga tatcaattca caatttaact catttgaata tgacggagtt
    16381 tcttttcaac ttaaaaatat gatggaggat aaatcgagag ttcatatact aaacggaatg
    16441 tataataaac cctcggtgtt ttttttcttt gctgttattg ttgtgttaat tataattttt
    16501 tccctctctt taaagaaaga tgaagtaaaa gaaattgctg aaattataga tgataagaga
    16561 tatggaatag tcaacacagg acagtgcaat tatattcttg ccgaaacaca aaatgatgcg
    16621 gtatgggcat ctgtagcctt aaataaaacc ggatttacca aatgcagata tattctggtg
    16681 tccaacaaag aaataaatag aattcaacaa tatatcaatc aacgattccc atatatcaat
    16741 ttgtatgtgt tgaatttagt gagtgataaa gcggaattac tggtattttt aagtaaagaa
    16801 agaaatagtt caaaagatac cgaacttgag aaattgaaaa atgctctgat tgttgaattt
    16861 ccgtatatca aaaatattaa attcaactat cttagcgatc ataatgcaag ggaggatgct
    16921 aaaggtattt tcacaaaggt aaatgtccag tataaagaga tttgtgaagc taataaagtt
    16981 acatattctg tcagagaaga actgacagat gaaaagcttg agctcattaa tagactgatt
    17041 tctgatcata aaaatgtata tggagatcag tatattgagt tttctgtact tttgatagat
    17101 gatgatttta aaggtaaatc atatcttaac agcaaagaca gttatgtaat gttgaatgat
    17161 aaacactggt tttttttaga taaaaataag tgaggataaa atgagtgtta cagtaccgga
    17221 taaagattgg actctgagtt cattatctga aacttttgat gatggaactc aaacattaca
    17281 aggtgaacta acattggcac tagatgcatt agctaaaaat ccttcgaatc cacagttgct
    17341 ggctgaatac caaagtaaat tatctgaata tacattatat aggaacgcgc aatctaatac
    17401 tgtgaaagtg attaaggatg ttgatgctgc aattattcaa aacttcagat aatagggagc
    17461 attcatgaat tacatttatc cagtcaatca ggttgatatt atcaaagcca gtgattttca
    17521 atctcaagag atatcaagtc tggaagacgt cgtgtcggct aaatatagtg atattaagat
    17581 ggatacagat attcaagtat cacaaataat ggagatggta ggcaatccag aatcattaaa
    17641 tccagaatct ttggccaagt tacagacgac gctctcaaat tattcaatag gagtatcatt
    17701 agctggcacg ttagcaagaa aaacagtttc ggctgttgaa actttattaa agtcttaatt
    17761 tatatgatta ggtataaagg ttttatttta ttcttgttgc tgatgttgat tggatgtgag
    17821 caacgtgaag agttaatttc taatttatct caaagacagg caaatgaaat aatatctgtg
    17881 ctagaacgcc ataatattac tgctagaaaa gttgatggag gtaaacaggg gatctcggta
    17941 caagtcgaaa aggggacatt tgcatcggca gttgatttga tgcgcatgta cgatttgcca
    18001 aatccggaga gagttgatat ctcacaaatg tttcctacag attcattagt gtcttctcca
    18061 agagctgaaa aggcccgttt atatagtgct attgagcaac ggctggaaca gtctttagtt
    18121 tctattggtg gtgttatttc ggcaaaaata catgttagct atgatcttga agaaaaaaat
    18181 atatcttcaa aaccgatgca tatatcagta atcgctatat atgactcacc gaaagagtct
    18241 gaactattag ttagtaatat taagcgattt ttgaaaaaca ccttttctga tgttaagtat
    18301 gaaaatatat ctgtcatatt aactccgaaa gaagaatatg tttatacaaa tgtacaacct
    18361 gttaaggaaa ttaaatcgga atttttaaca aatgaagtaa tatatgtatt tctcgggatg
    18421 gctgtactag ttgtcactct tttggtatgg gcattcaaaa cagggtggtt caagagaaac
    18481 aaaatatgat aagaatggat ggaatttata aaaaatatct ttcaataatt tttgatccag
    18541 cgttctatat aaatagaaat cggttgaatt tgccttctga actgttagaa aatggcgtaa
    18601 tcagaagtga gattaataat ctcataatta ataaatatga tctaaattgc gatattgaac
    18661 ctttaagcgg ggtaaccgct atgtttgttg ccaactggaa tttacttcca gctgttgctt
    18721 attttatcgg tagtcaagag tctcggttaa ttaatcatag tgagatggtt attagttatt
    18781 atgggggcaa aatcagtaag caaggggagg ccgctattcg aagtggtttt tggcatctta
    18841 ttgcgtggaa ggaaaatata tctgttggta tatatgaaag aataaactta ttatttaatc
    18901 caattgcttt ggagggaaat tatacgcctg tagaaagaaa tttgtctcga ttaaatgaag
    18961 gtatgcaata tgcaaaaagg cactttaccg gtatccagac atcatgccta tgatggtgtt
    19021 gttataaaga gaattgaaaa ggaattatgt aaaaccataa aagatagaga cactgagagt
    19081 aagaagaaag caatttgtgt tataaaggat gctacgaaaa aagctgaatc actgcgtata
    19141 gatgctgtat gtgatggtta tcaaattggt atacagactg cttttgagca tattattgac
    19201 tatatttgtg aatggaagtt aaagcaaaat gaaaacagaa gaaatattga ggactatata
    19261 actagtttgc tttcagaaaa tttacatgat gagaggatca ttaacacttt attggagcag
    19321 tggcttagtt cgttgcgtaa tacagtaaca gaattaaaag tggttttgcc taagtgtaat
    19381 ttagcactga ggaaaaagtt agaacttgat ctccataaat atcgctcaga tgtcaagatt
    19441 attttaaaat attctgaagg gaataattat atattctgta gtggtaatca agtagtggag
    19501 tttagtcctc aggatgtaat atcaggtgtt aaaatagaac ttgcggaaaa attaacaaaa
    19561 aatgataaaa aatatttcaa agagttagca cataagaaac taagacaaat tgcagaagac
    19621 ttgttaaaag agaatcctgt taatgattaa tcaaataaat gcaagcaatg ctctgcagca
    19681 aagattaaat agtgaagaat ttgttaattt aaatgagaga ttatcatctt cgcaatcatt
    19741 tgatgaggat ataatctatg aaataatgca atatttctca cagagtgaat taaattccat
    19801 agataatgat gaactgcaca ataagattga acaattattc aattcacgtt ttccttatct
    19861 tacagctgca caaaaaagtt cacttcttaa taaactcata gatgcaaatc aatatgtcga
    19921 tttgcatgag ggattttatg catcattgtc catatataac aatattgatt tctatattaa
    19981 gactacaacc ttcgattcat taattagtgt ctttgacgca gggagagagg cagatgattc
    20041 gacatggtag taataagttg aaaatattta ttttaagtat attgctatta acactgagtg
    20101 ggtgtgcttt aaagtcatca tctaattctg aaaaagaatg gcatattgtt cctgtaagta
    20161 aggattattt ttctattcca aatgatttat tatggtcgtt taatacaacc aataaaagta
    20221 taaatgttta ctctaaatgt attagtggta aggcggttta tagttttaat gcaggtaaat
    20281 tcatgggcaa ctttaatgtt aaggaagtag atgggtgctt catggatgca caaaagatag
    20341 ctatagataa actattttct atgctgaaag acggggttgt tttaaaaggt aataagataa
    20401 atgataccat ccttatagag aaggatgggg aagttaaatt aaaattaatt cgagggatat
    20461 aattgtattg tgagtaaata taaaggtcta aatacaagta atatgtttta catttactct
    20521 agtggacatg aaccagttaa cgttgagctt gtaaaagata aagaacgtaa cataattgag
    20581 ctggctccag catggaaggg tttttttttg tccgaaatca aaatataaaa ttcagtgata
    20641 atgtgaacta tcattatcgc tttaacatta atagttgtgc aaaatttctt gctttttggg
    20701 actatttcag tggtgctcta gtagagcata gccatgctga aaaatgtatt catttctatc
    20761 atgagaatga tcttcgggat agttgtaata ccgaatcgat gcttgataag ttaatgttaa
    20821 gattcatctt ttcatctgac cagaatgtaa gtaatgcact ggctatgata cgaatgactg
    20881 agtcatatca tttggttctt tatcttctga gaactattga gaaagaaaag gaggtaagaa
    20941 taaagagtct gacagagcat tatggtgtgt cggaggctta ttttagatct ttatgtagaa
    21001 aagctttagg ggcaaaagtc aaagaacaac tgaacacttg gcggttagta aatggtttat
    21061 tagatgtatt tttgcacaat caaacaatca cttctgctgc catgaataat gggtatgcat
    21121 caacatccca tttttcaaat gagataaaaa cacgactagg cttttctgca agagaattat
    21181 cgaatattac ttttttagtg aaaaaaataa atgaaaaaat ttaatattaa atctttgact
    21241 ctcttgattg tattgttacc attgatagtt aatgctaata atattgactc tcatttatta
    21301 gaacaaaatg acatagcgaa atatgttgct caaagtgata cggtaggatc tttttttgaa
    21361 cgtttttctg cgttattaaa ttatcctatt gttgttagca aacaagctgc aaaaaagaga
    21421 atatctgggg agtttgattt atctaatcca gaagaaatgc ttgaaaaact gacattactt
    21481 gtgggactca tatggtataa agatggaaat gcattataca tatatgactc tggagaatta
    21541 ataagcaaag ttattttgct ggagaatata tcacttaatt atcttattca gtatctgaag
    21601 gatgctaacc tttatgatca cagatatcct ataagaggaa acattagtga taaaacattc
    21661 tatatatctg gtcctccggc attggttgaa ctggtagcta atacagcaac tttgcttgat
    21721 aagcaggttt cttctattgg gacagataaa gttaattttg gggttataaa attaaaaaat
    21781 acatttgtta gtgacagaac ttataatatg agaggggagg atattgtcat cccaggggtg
    21841 gctactgttg tagaacgttt attaaataat ggtaaggctc tgagtaacag acaagccaaa
    21901 aatgatccga tgccaccctt taatattaca cagaaagtat ctgaggatag taatgacttt
    21961 tcatttagtt cagttaccaa tagttctata ttagaggatg tatcacttat tgcatatcca
    22021 gaaaccaatt ctatattagt caaaggtaat gatcagcaaa tacagattat tcgcgatatt
    22081 atcactcagt tagatgtagc caaacgacat atagaattat cattatggat aatcgatatt
    22141 gataaatctg aattaaataa ccttggcgtt aattggcaag gaacggcttc atttggtgat
    22201 tcgtttggtg cgtcatttaa tatgagctca tcagcatcga taagcacttt ggacggcaat
    22261 aagtttattg cttcagttat ggcattaaat cagaaaaaga aagcaaatgt agtttcaagg
    22321 ccggtgattt tgacacaaga gaatattccg gctatatttg ataataacag gactttttat
    22381 gtgtcattag ttggtgagcg caattcttcg ttggagcatg ttacgtatgg aacattgata
    22441 aatgttatac ctcgcttttc atctcgggga caaattgaga tgtctttaac gatagaggat
    22501 ggaacaggaa atagtcagtc aaattacaat tataataatg aaaatacatc tgtccttcca
    22561 gaagttggaa ggaccaaaat cagtacaata gcgagagttc cacaaggcaa aagtttgttg
    22621 ataggtggtt atacgcatga aacgaactct aacgaaatag tatctattcc ttttctctcc
    22681 tctattcctg taatcggtaa tgtgttcaag tataaaacaa gtaatatcag taatattgta
    22741 agggtttttc tgattcaacc ccgtgagata aaggagtcaa gttattataa tactgcagaa
    22801 tataagagcc tcatatctga aagagaaata caaaagacta cacaaataat cccatctgaa
    22861 actactttgc tggaagacga aaaatcattg gtttcatact taaattacta actataaagt
    22921 aggtgatgta tgcttgatgt taaaaataca ggagttttta gctctgcatt cattgataag
    22981 ttgaatgcaa tgacaaattc agatgatgga gatgagactg ctgatgcaga gcttgattct
    23041 ggcttggcta atagcaagta tattgactca tctgatgaga tggcttccgc tctttcgtca
    23101 tttataaaca gaagagacct tgagaaactg aaaggaacaa atagtgatag tcaggaacgt
    23161 attttagatg gggaagaaga tgaaattaat cacaagattt ttgatttaaa gagaacttta
    23221 aaagataacc ttcctctaga tcaggatttt atagacagac taaagagata ttttaaagat
    23281 ccaagtgatc aagtcttagc attaagggaa cttttgaatg aaaaagatct tactgctgaa
    23341 caagtcgaat cattaactaa aattattaat gagataatat caggtagtga aaaaagtgtt
    23401 aatgctggaa taaattcagc tatacaggct aaattatttg gcaacaaaat gaaacttgaa
    23461 ccacagcttt tgcgtgcatg ttatcgtggt tttatcatgg ggaacatatc aacaacagat
    23521 cagtatatag aatggcttgg taattttggt tttaatcaca gacatacaat tgtgaatttt
    23581 gtagagcagt cactgattgt agacatggat tctgagaaac cgagctgtaa tgcttatgag
    23641 tttggttttg tgttatctaa attaattgca attaagatga ttagaacttc agatgtaatt
    23701 tttatgaaga aactggaatc ctcaagcttg ctaaaagatg gcagtttaag tgcagagcag
    23761 ctattgctaa ctttattata tatttttcaa tatccaagtg aaagtgagca aattcttact
    23821 tctgttatag acgtatcacg agccagtcat gaggattctg tagtgtatca aacatatcta
    23881 tcttctgtta atgaaagtcc tcatgatata tttaaaagtg aaagtgaaag agaaattgcg
    23941 atcaatattc tacgagagct tgtcacaagt gcatacaaga aagagctttc tagataacag
    24001 gagatgaaag tgatccagtc ttttcttaag caagtaagta ctaagcctga attaattata
    24061 ttagtgctta tggttatgat cattgctatg ttgatcatac cattacctac ataccttgtt
    24121 gattttttaa tagggcttaa tattgtactt gctattctcg tttttatggg atcattttat
    24181 attgaaagaa tattgagttt ttctacattc ccttcggttt tgttgataac gacattattt
    24241 cgtctcgctt tatcaataag tactagtcga ctaattttag ttgatgcgga tgcgggtaaa
    24301 attattacta catttggcca gtttgtcatt ggtgatagtc tggctgtagg ttttgttatt
    24361 ttttcaatag taactgttgt gcagttcatt gttataacaa aaggatctga aagggttgcg
    24421 gaggtagctg ctcgcttttc tcttgacggt atgccgggga aacaaatgag cattgatgcg
    24481 gatttaaagg ctggaattat tgatgccgca ggagctaaag aaagacgcag tattctggaa
    24541 cgtgagagtc aattgtatgg ttcatttgat ggagcaatga agttcattaa aggtgatgca
    24601 attgctggca tcattattat ttttgtcaat ttaataggag gtatttctgt tggtatgagt
    24661 caacatggaa tgtctctatc tggtgcatta tctacttata ccatactaac tattggtgac
    24721 ggattggtct ctcagattcc tgctctgtta atttctataa gtgcaggatt tatcgttaca
    24781 agagttaatg gtgatagcga taatatggga cgtaacataa tgtcccagat atttgggaat
    24841 ccttttgttc tcattgttac atcagctctt gccttggcga tagggatgtt gccaggtttt
    24901 ccgttttttg tttttttcct gatagcagtt actttgacgg ctttattcta ttataaaaag
    24961 gtcgtagaaa aagaaaaaag tctgtctgag tctgattcta gtggctatac tggtacattt
    25021 gatattgata atagccatga ctcatctttg gcaatgatag aaaatctgga tgctattagt
    25081 tcagaaaccg ttcctttaat tttattattt gccgaaaata agataagtgc aaatgatatg
    25141 gaaggtctta ttgaaaggat aagaagtcag ttctttattg attatggtgt caggcttcca
    25201 actattttat atagaacaag caatgagctt aaggtcgatg atattgtttt gttaataaat
    25261 gaggtgcgtg ccgatagttt taatatatac tttgacaaag tgtgtattac agatgaaaat
    25321 ggagatatag atgctctagg tattcctgtt gtttcaactt catataatga acgtgttatt
    25381 tcttgggtag acgtttcata tacagaaaat ctaactaata ttgatgctaa aattaaaagt
    25441 gctcaagatg aattttatca ccagttgtca caagctttat taaacaacat aaatgagatt
    25501 tttggtatac aagaaacaaa aaatatgtta gatcagtttg aaaatcggta tcctgatcta
    25561 ttaaaggaag tcttccgaca tgtgactata cagagaattt ctgaggtatt acaaagattg
    25621 cttggagaaa atatttctgt tcgcaattta aaacttatta tggagtcttt ggcgctttgg
    25681 gctcccagag aaaaagatgt tataacatta gttgaacatg tccgtgcatc actttctagg
    25741 tatatttgta gtaaaatagc tgtttctggt gagattaaag ttgtgatgct ttccggatat
    25801 attgaggatg caataagaaa agggataagg caaacctctg gtggctcttt cttgaatatg
    25861 gatatagagg tttcggatga ggtaatggaa actttagcac atgctttgag agaattgaga
    25921 aatgcaaaaa aaaatttcgt tcttttggta tcagtagata tacgtaggtt tgttaaaaga
    25981 cttatagata acagatttaa gagtatactc gttatatcgt atgctgagat tgatgaagca
    26041 tataccatta atgtattaaa gactatttag tgaggtttaa atatgagtaa cattaattta
    26101 gttcaattag ttagagatag tcttttcacg attggttgtc ctccatcaat tattacagat
    26161 ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat taatatagct
    26221 cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga tgtaaaactt
    26281 cagtcatctg cttataacat attaaattta atgttaatga actttagtta tagcattaat
    26341 gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt tattaaagat
    26401 gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta tcagaggatg
    26461 gaaatcttaa atgaggtctt ataatcaatg agctatataa aattgctcac tcaattatct
    26521 tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt ttcgattggt
    26581 gagatttgta acattcaggc tggaattgaa agtaatgaaa ttattgcaag agctcaggtt
    26641 gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg tggactttca
    26701 cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg ccgtggatta
    26761 ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt tacggataac
    26821 agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga aagggctgca
    26881 attgagaagc cttttttaac aggtgttaag gttattgatt ctttactcac gtgtggtgaa
    26941 ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct catgaatatg
    27001 ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga gcgaggtcga
    27061 gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaagcag gtgtgtttta
    27121 gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata tatagccact
    27181 gctatagccg aattttttag gactgaagga cataaagtag cgctttttat tgattcatta
    27241 acaaggtatg ccagagcatt acgtgatgtg gccttagcag ctggagaatc acctgccaga
    27301 agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag gccaggaaag
    27361 ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga tgatgatttt
    27421 gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata tttgagcaga
    27481 aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat aagcagggta
    27541 tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg ggagctactt
    27601 tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc gggggagaat
    27661 gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct gaagcaagat
    27721 tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac aattagataa
    27781 ggttttaaaa atcaaagata aatatcaacg gtctgttaaa ttaattgaag cacacatatt
    27841 aacactaaaa aaaaaactct ttgtatcgag atgtggaggc attagataaa aggattatat
    27901 acttccagca actggagaat gacttggaac ctgtaggcgc tcagagtgtt agtcagcttt
    27961 tcaatacaag acgtaagatc gctatagtaa aaaagcatat tatccaatat cagtctgaac
    28021 gaattcttct aaagggaaga attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt
    28081 ctaaaagaaa actgcttcat aaagagagta aaatatgtaa acggattggc ttgattaaaa
    28141 gaaataactt tgccaaacag ttgatacttg atgagttatc acaagaggat atgaagtatg
    28201 gcattagata atataaacct aaattttagt agtgacaaac agatagaaaa atgtgagaaa
    28261 ctatcttcaa tagataatat tgatagtctc gttttgaaga agaagaggaa ggttgaaatt
    28321 cctgagtact ctttaattgc atctaactat tttactattg ataagcactt tgaacataag
    28381 catgataaag gagaaattta tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga
    28441 gcgacatatt ctgatattcc agaatcaatg gccattaaag aaaatatttt gataccagat
    28501 caagatatca aagcaaggga aaaaataaat atcggcgata tgagggggat cttttcatat
    28561 aataagagtg gaaatgcaga caagaacttc gaaagaagtc atacttcttc tgtaaaccct
    28621 gataatctgc tagaatctga taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg
    28681 tctattgata agaatattgc tgacatcatt tctttactaa atggaagtgt tgctaaatca
    28741 tttgagctgc ctgtaatgaa taaaaatact gcagacataa ccccatccat gtcattgcaa
    28801 gaaaaatcaa tagttgaaaa tgataaaaat gtttttcaaa aaaatagtga aatgacttac
    28861 cactttaaac agtggggggc tggacattct gttagtattt cagtggagtc tggttctttt
    28921 gttctaaaac cgtcagatca atttgtagga aataaacttg acttaatttt gaaacaagat
    28981 gctgagggta attacagatt tgatagcagt caacataata aggggaataa aaataatagt
    29041 acaggatata atgaacagag tgaagaagaa tgctaagaat taaacatttt gacgctaacg
    29101 aaaaactaca gattttatat gcaaagcaac tctgcgagcg tttttcgatt cagacattca
    29161 aaaataaatt tacaggcagt gaaagtttag tcactcttac ttccgtgtgt ggggattggg
    29221 taattcgtat tgatacatta tcttttttga aaaaaaaata cgaggtattt tcaggatttt
    29281 ctacacaaga atctttactg catttatcaa aatgtgtctt tatagagtcg tcatctgtat
    29341 tttcgattcc agaactgtct gataagatta ctttccggat cacgaatgaa atccagtatg
    29401 caactactgg gagtcattta tgctgttttt cctcttcttt aggtattatt tattttgaca
    29461 agatgccggt attacgtaat caagtttctc ttgacttatt gcatcatctt ttagagtttt
    29521 gcttaggttc atctaatgta aggctggcta ctttaaaaag aattcgtact ggtgatataa
    29581 tcatagttca gaaactttat aatttattat tgtgtaatca agttattatt ggggattata
    29641 ttgtgaatga taataatgag gcaaaaatta atctgtcaga aagtaatggt gagtcagatc
    29701 acacagaagt ttctttggca ttattcaatt atgatgatat caatgtaaaa gtggacttta
    29761 tccttttaga aaaaaagatg acaatcaatg aactaaaaat gtatgtagaa aacgaattat
    29821 tcaagtttcc cgatgacata gttaaacatg taaatattaa agtaaatggt tctttggttg
    29881 ggcatgggga acttgtttct attgaggatg gttatggtat cgagattagt tcttggatgg
    29941 taaaggagta atctcatgct gagtgatatg tccctcatcg ctacactttc tttttttacg
    30001 ctattaccat ttttagttgc tgctggtact tgttatataa agttttctat tgtttttgta
    30061 atggtacgaa atgcacttgg tttgcagcag gttccatcaa atatgactct caatggtatt
    30121 gcattgatta tggctttgtt cgtgatgaaa cccattattg aagctggtta tgaaaattat
    30181 ctaaatggcc cgcaaaaatt tgatacaata tcagatattg tccgtttttc tgattctggc
    30241 ttgattgaat acaagcaata cttgaaaaag catacagatc ttgagttggc acgttttttc
    30301 caaaggtctg aagaagaaaa tgctgattta aagagtgcag agaataatga ttattcattg
    30361 ttttccctct tgcctgcata tgcgctaagt gaaattaaag atgcgttcaa aataggtttt
    30421 tatttatatt tgccatttat tgttgttgat cttgttattt caagcatttt acttgcactt
    30481 ggtatgatga tgatgagtcc aatcacaata tcagtaccaa taaaacttgt attatttgtt
    30541 gcgcttgatg ggtgggggat cctgtctaag gccttgattg aacaatatat caatgttcct
    30601 gcttagatga tggaggtaat gtgtctgata tagtttatat gggtaataag gctctttatt
    30661 taatccttat cttttcctta tggccagtag gtatagctac ggttattgga ttaactattg
    30721 gtttattaca gacagtgact caacttcaag agcagacact tccttttggt ataaagctta
    30781 taggtgtctc aatatctttg ctacttcttt ctggatggta tggtgaggtt ttattgtctt
    30841 tttgtcatga aataatgttt ttaattaaga gtggggtttg atggacattt caagctggtt
    30901 cgaaagtatt catgtgtttt taatactcct gaacggcgtt ttttttagat tggctccatt
    30961 atttttcttt cttccatttt taaataacgg tataatttct ccatctatta gaatacctgt
    31021 gatttttctt gttgcatcag gattaattac ttctggtaag gtagacatag gttcttctgt
    31081 ttttgaacat gtttatttcc ttatgttcaa ggaaataatt gttggcctcc ttctctcttt
    31141 ttgcttgtct cttccctttt ggatatttca tgctgttggt agcattattg acaaccagcg
    31201 tggggcaacg cttagtagtt caattgatcc tgccaatggt gttgatacgt ctgagttggc
    31261 aaaatttttc aatctttttt ctgcagttgt atttctatac agtggtggaa tggtctttat
    31321 tttagaatcc atacaattgt cttataatat atgcccgtta ttttctcaat gttctttccg
    31381 tgtctcaaat atcttaacat ttctgacttt attggcaagt caggctgtta ttttagccag
    31441 tcctgttatg atagtattgt tactatcaga agtattactt ggtgtattat cgagatttgc
    31501 tccgcagatg aatgcttttt ccgtatcatt aactattaaa agtttacttg caatatttat
    31561 tattttcatc tgttcttcta ctatttactt ttctaaagtt caatttttcc tcggtgaaca
    31621 taaatttttc acaaatctat ttgttagata aaatattatg gcaaataaaa cagaaaagcc
    31681 gacacctaaa aaactaaagg atgccgcaaa aaaaggacag tcatttaaat ttaaggattt
    31741 aacgactgtt gttattattc tggtagggac atttactata atatcattct tttccttaag
    31801 tgatgtaatg ctcttataca gatatgtaat aattaatgac ttcgaaatta atgagggtaa
    31861 atactttttt gcagtggtta ttgtcttttt taagataatt ggcttcccac tttttttctg
    31921 tgttctttcg gctgtgttgc caacattggt tcaaacaaag tttgttcttg cgactaaagc
    31981 tatcaagatt gatttttcag tattaaaccc tgttaaaggg ttaaaaaaaa tatttagtat
    32041 aaagacaata aaagaatttt tcaaaagcat tctgcttctt attattctag cattaacaac
    32101 ctatttcttt tggattaatg accgaaaaat aattttttct caggtgtttt ctagtgttga
    32161 tggcttatat cttatttggg gggggctgtt taaggatata atattatttt tcttggcatt
    32221 ttctattttt gttattattc ttgactttgt gattgagttc attttataca tgaaagatat
    32281 gatgatggat aaacaggaga taaaaagaga atatatagag caagagggac actttgagac
    32341 aaagtcgaga aggcgtgagt tgcatatcga gattctttca gagcagacta aatctgatat
    32401 acgtaattca aaattagtgg taatgaaccc gactcatatt gcaattggta tttattttaa
    32461 tccagaaata gcgcctgcac cttttatttc tctcattgaa actaaccagt gtgccttggc
    32521 tgtcagaaaa tatgcaaatg aagttggtat accaactgtg cgtgatgtga aattagctag
    32581 aaaactatat aaaacacata caaaatatag ttttgttgat tttgaacact tggatgaagt
    32641 cctacgtctt attgtttggc ttgagcaggt tgaaaacact cattaataag taaaggagat
    32701 gtgttatatg ggagttaatt tctgtaataa aataggtatt gatcagtctg aatttgaaat
    32761 agaatcttcc atcattaact ccattgctaa tgaggtattg aacccaatat ctttccttag
    32821 caacaaggat ataataaatg ttttactcag aaagatttca tctgaatgtg acctagttag
    32881 aaaagacatt tatcgttgtg ctctggagtt ggtcgttgaa aaaacacctg atgatttata
    32941 atcattcata ataactcttt tgctgatata aatatttttg ttgaagatat ttatgtagtc
    33001 acaggatcaa ttgtagaaaa ttgttatgtt tttgttagga actgcactaa ataatatatg
    33061 ataagacagc aaaaacgact gaccataatc cttcttttat taggagttga taagagagac
    33121 tattcatcct gtaatgttaa aactctgtta tatagtattc gcgattatgc aaagtctgta
    33181 aatgatcatg agatactaac cgagagtaat cgtttattat ctcattgcat ttcagatagt
    33241 aacggagctt tctttaaaag tagtaaatac gttcccctta agtacttacg taaaaggaga
    33301 atagcacgaa agataccaaa tgattagcac tttgctagta agcttgctgg taaactatat
    33361 tgaccgttgg gtttgttttg agactgtaat ttaaattgtg taattgcctg tttttgatat
    33421 gtttactcca ac
//
LOCUS       DADLSE010000034        32298 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLSE010000034 DADLSE010000000
VERSION     DADLSE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.034"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN692_001562"
     CDS             <1..848
                     /locus_tag="JN692_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7263903.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLAPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASADLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVAWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
     gene            821..2357
                     /locus_tag="JN692_001563"
                     /pseudo
     CDS             821..2357
                     /locus_tag="JN692_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2357..3010
                     /locus_tag="JN692_001564"
     CDS             2357..3010
                     /locus_tag="JN692_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7263904.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            3077..4384
                     /locus_tag="JN692_001565"
     CDS             3077..4384
                     /locus_tag="JN692_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7263905.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVENEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(4393..5013)
                     /locus_tag="JN692_001566"
                     /pseudo
     CDS             complement(4393..5013)
                     /locus_tag="JN692_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            5100..5507
                     /gene="nudG"
                     /locus_tag="JN692_001567"
     CDS             5100..5507
                     /gene="nudG"
                     /locus_tag="JN692_001567"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7263906.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(5473..5745)
                     /locus_tag="JN692_001568"
     CDS             complement(5473..5745)
                     /locus_tag="JN692_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7263907.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            5981..7324
                     /gene="gdhA"
                     /locus_tag="JN692_001569"
     CDS             5981..7324
                     /gene="gdhA"
                     /locus_tag="JN692_001569"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7263908.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(7441..8481)
                     /locus_tag="JN692_001570"
     CDS             complement(7441..8481)
                     /locus_tag="JN692_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7263909.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSATLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(8609..10570)
                     /locus_tag="JN692_001571"
     CDS             complement(8609..10570)
                     /locus_tag="JN692_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7263910.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN692_001572"
     CDS             complement(10575..11618)
                     /gene="selD"
                     /locus_tag="JN692_001572"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7263911.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(11735..12286)
                     /locus_tag="JN692_001573"
     CDS             complement(11735..12286)
                     /locus_tag="JN692_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7263912.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGGERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            12447..14303
                     /gene="sppA"
                     /locus_tag="JN692_001574"
     CDS             12447..14303
                     /gene="sppA"
                     /locus_tag="JN692_001574"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7263913.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSPHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            14470..15486
                     /gene="ansA"
                     /locus_tag="JN692_001575"
     CDS             14470..15486
                     /gene="ansA"
                     /locus_tag="JN692_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7263914.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNASQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            15497..16138
                     /gene="pncA"
                     /locus_tag="JN692_001576"
     CDS             15497..16138
                     /gene="pncA"
                     /locus_tag="JN692_001576"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7263915.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16231..17589)
                     /locus_tag="JN692_001577"
     CDS             complement(16231..17589)
                     /locus_tag="JN692_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263916.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17707..18465)
                     /locus_tag="JN692_001578"
     CDS             complement(17707..18465)
                     /locus_tag="JN692_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7263917.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(18602..19582)
                     /gene="ydjG"
                     /locus_tag="JN692_001579"
     CDS             complement(18602..19582)
                     /gene="ydjG"
                     /locus_tag="JN692_001579"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7263918.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKLIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(19592..20539)
                     /locus_tag="JN692_001580"
     CDS             complement(19592..20539)
                     /locus_tag="JN692_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7263919.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(20544..21380)
                     /locus_tag="JN692_001581"
     CDS             complement(20544..21380)
                     /locus_tag="JN692_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7263920.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            complement(21401..22444)
                     /locus_tag="JN692_001582"
     CDS             complement(21401..22444)
                     /locus_tag="JN692_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HAY7263921.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLAMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(22461..23840)
                     /locus_tag="JN692_001583"
     CDS             complement(22461..23840)
                     /locus_tag="JN692_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7263922.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLLAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(23867..24943)
                     /locus_tag="JN692_001584"
     CDS             complement(23867..24943)
                     /locus_tag="JN692_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7263923.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSSANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(25313..25585)
                     /locus_tag="JN692_001585"
     CDS             complement(25313..25585)
                     /locus_tag="JN692_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7263924.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(25627..26040)
                     /gene="msrB"
                     /locus_tag="JN692_001586"
     CDS             complement(25627..26040)
                     /gene="msrB"
                     /locus_tag="JN692_001586"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7263925.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            26382..27377
                     /gene="gapA"
                     /locus_tag="JN692_001587"
     CDS             26382..27377
                     /gene="gapA"
                     /locus_tag="JN692_001587"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7263926.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            27461..28345
                     /locus_tag="JN692_001588"
     CDS             27461..28345
                     /locus_tag="JN692_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7263927.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(28396..29157)
                     /locus_tag="JN692_001589"
     CDS             complement(28396..29157)
                     /locus_tag="JN692_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7263928.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(29247..29993)
                     /locus_tag="JN692_001590"
     CDS             complement(29247..29993)
                     /locus_tag="JN692_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7263929.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            30429..>32298
                     /gene="yeaG"
                     /locus_tag="JN692_001591"
     CDS             30429..>32298
                     /gene="yeaG"
                     /locus_tag="JN692_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7263930.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEK"
BASE COUNT         8107 a         8368 c         7754 g         8069 t
ORIGIN      
        1 taaaagaggc caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg
       61 tcgacacaca gctgccggtg cgggaagatt tttcagtgcc gacacaaggt gcggaatcgc
      121 cctggggcgg cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac
      181 aaacgccgca agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc
      241 gcccaccgga ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctggcgccag
      301 atcccgccgc attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct
      361 tgtggcaata tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc
      421 cttcgcccgc gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct
      481 ttaaccccgc gcatgcgcag caaaaaatcg ccagcgccga tttgccggca agcagttaca
      541 gttttggctt tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg
      601 cgaatgccag tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac
      661 tgcgtaaagc agatcccgtt gcctggggcg atccttctgt tctcgatccg caaaaactgc
      721 ctgacgggca gcgcgaaata ttgcaatcaa gaatgccgca ggatctgccg ccggtactgg
      781 ctgaaccgca cgcaggatgg gtgaatgcac tggaacaaga atggttacgc cgttacggta
      841 cgcattaatc tttttgctgt gggcgatggt ggcggtgatt tatgcaccgc tgatcccggc
      901 agctctcacg ctgatatcgc ctgcgttgtc gttgacacgc tggcaggcgt tatttgccga
      961 tccacagtta ccgcaggcat tactggcaac gctggtgtcg acaaccatcg cggcggtcgg
     1021 ggcattgttg attgccctgc tggtgattgt ggcgctatgg cctgggccga aatggcagcg
     1081 tatgtgcgcc cgtctgccgt ggctgctcgc cattccccat gtggcttttg ccaccagcgc
     1141 cctgctgctc tttgctgacg gagggctgct atatgactat ttcccgtatt tcactccgcc
     1201 aatggaccga tttggcatcg gtctgggcct cacccttgca gtgaaagaaa gcgcatttct
     1261 gctgtggatc ttagcggcag tattgagcga aaaacggctg ttgcagcagg tcattgtgct
     1321 ggattcgctg ggctacagcc gctggcaatg cctgaactgg ctgctgttgc cctccgtcgc
     1381 gccttcgctg gcaatggcga tgctggcgat tgttgcctgg tcgctttcgg tcgtggatgt
     1441 ggcgattatt cttgggccag gtaatccacc gacgctggca gtaattagct ggcagtggtt
     1501 aactcagggc gacgcggatc aacaaacgaa aggcgcactc gccagtctgc tgctgatgct
     1561 gttactcgcc gcctacgttt tgctgagcta tctgctatgg cgcagctggc ggcgcactat
     1621 tccccgcgta gatggcattc gcaatgcctg ccacaccttt attaccaggc actacgctgg
     1681 cgagtttttt acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc
     1741 agtcgacgat caatagtgaa gcactcatca acagtctgac aatggggctg gtagcaacat
     1801 tcatcgcttt gctcctgtta ctgctgtggc tggaatgggg ggcacagcgt cgccagttgt
     1861 ggctatggtt gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg
     1921 cgctatggct gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg
     1981 taatgccgtg gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa
     2041 ttttgattgc gcaaacactg ggctggtcgc gggccaaaat cttcttttac gtgaaatgcc
     2101 cactcatgtt gcgccctgtg ctgattgcct tcgcggtggg attttcagtc agtattgcgc
     2161 agtatatgcc gacgctgtgg ctgggcgcgg ggcgttttcc gacgctcacc actgaggcgg
     2221 tggcattaag cagcggcggc agcaacggta ttctcgccgc ccaggcttta tggcaactgt
     2281 tgttaccgct tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca
     2341 gacaaggaat ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg
     2401 cttgctgaca aacgttaact ttacggttga taaaggtgac atcgtcacgt taatggggcc
     2461 gtctggctgt ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ccggacagtt
     2521 ttcttgtaca ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca
     2581 gcgtcagatt ggcattcttt ttcaggatgc actgttattt gaccagttca gcgtcgggca
     2641 aaatttactg ctggcgctac cggcgacact taaggggaat gcccgacgta atgccgtgaa
     2701 tgatgcactt gagcgttcag gccttgacgg agctttccat caggatcctg ccactttgtc
     2761 tggcggtcag cgagcgcgcg ttgctctact acgcgccctt ctcgcccagc caaaagcgtt
     2821 actcctggat gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg
     2881 ggtgttcagc gaagttcgcg ccctggcgat ccccgtcgtt caggtaacgc acgatctcca
     2941 ggatgttcct gctgatagtt ctgttctgga tatggcgcag tggtcagaaa attacaacaa
     3001 actgcgataa cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac
     3061 aatgtcggga ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg
     3121 gctggcttgc gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccaact
     3181 tcagcaacaa aatggcaaag cgatagatac tcgccccagc gcgttttata acggctggcc
     3241 acaaacctta aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgctagctg
     3301 gctcgacaaa atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc
     3361 cgatgccccg gtggcgctgt acggtaatgg caaagatgtc gacgccgtca aaacgcgact
     3421 gcaaaaagca ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg
     3481 tctacaaaaa ctgccgcact ttgagcagct ggtttatccg caatggctgc atgacctgca
     3541 acaaggtaaa gaggttacgg cgaaacctgt cggtgactgg aaagtcattg aagcggcctg
     3601 gggcgctcct aagctttacc ttatcagcca tattcctggc gctgactaca tcgataccaa
     3661 cgaagtggaa aacgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct
     3721 ggcaaaacac ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc
     3781 tggagcgcgt gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga
     3841 tggcggctgg caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa
     3901 agtgaaagcg gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga
     3961 tatggaacag gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc
     4021 gtggccagaa tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat
     4081 agccggagca cgttggggac acgctggtag cgactcgacg catatggaag atttccataa
     4141 cccggatggc accatgcgta gcgccgatga tattaccgct atgtggaaag catggaatat
     4201 caaaccagat cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt
     4261 tatgtacgca cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga
     4321 atggagcagc gatccaaaaa atccggttgc aaccggtgaa cgcggcccgg gcagtagcaa
     4381 ataacattgc aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg
     4441 cgtaaaggtc gtcatccagc acaacgcacc gaagattcac gcaaaccacg caaaccatgc
     4501 agggaataaa catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaatccccc
     4561 cagatagtaa aacgacttat gtgcataacc ggggttatca atctgatgtt ttgctgccag
     4621 tgcagcaaag gcgagaaaac tgctgcccgt gccgataaac gcaaacaaca accagccgcc
     4681 tgccagcgca ttttgctccg gtgcagcaag aataaagcca aacggcacca gcgcgtaaaa
     4741 gagaaaatcg agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc
     4801 cagcgcgccg tccagaccat caagcagcct gttcaacaaa atgacgacta gcgccgccag
     4861 ataccagccc agcgccagaa acggcagagc cagcacgccg atggcaaatc ccactaatgt
     4921 taaaccatct ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg
     4981 tttaatccgg ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc
     5041 tgattgagca acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta
     5101 tgaaaatgat tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc
     5161 aacgccccgc ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc
     5221 tggatgaaag ccagcagcag gcgctggtgc gtgagttaaa cgaagaattg ggcatcgaag
     5281 caactgtggg tgagtatgtt gccagccatc agcgagaagt ttcgggccgg attatccatc
     5341 ttcatgcctg gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc
     5401 tggtctggtg ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat
     5461 tattagaggc gtttatggct ttacgcgccg ccagagcagc ggattagtgc taagggtttt
     5521 gtcatcacgc tggcattgca gcagtattcc ttcggcttta attaccgccc cttcagaata
     5581 attttgatcc tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact
     5641 gaacacttct ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc
     5701 aattaatgga aatgctaata ctacggcgaa caatgctcga ctcacaggga actccttaac
     5761 gttattgtct ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat
     5821 cgcatttggt tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt
     5881 gcttaccgtc acattcttga tggtatagtc gaaaactgca aaagcacatg acataaacaa
     5941 cataagcaca atcgtattaa tatataaggt ttttatatct atggatcaga catattctct
     6001 ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc
     6061 cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca
     6121 gatgtcatta ttggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg
     6181 ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc
     6241 catcggcccg tacaaaggcg gtatgcgctt ccatccgtca gttaaccttt ccattctcaa
     6301 attcctcggc tttgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg
     6361 taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa ggtaaagtga tgcgtttttg
     6421 ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg
     6481 tgatatcggc gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc
     6541 caacaatacc gcctgcgtct tcaccggtaa gggcctttca tttggcggta gtcttattcg
     6601 cccggaagct accggctacg gtctggttta tttcacagaa gcaatgttaa aacgccacgg
     6661 tatgggtttt gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc
     6721 tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac
     6781 tgtagttgat gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc
     6841 cagccgcgat ggtcgagtgg cagattacgc caaagaattt ggtctggtct atctcgaagg
     6901 ccaacagccg tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact
     6961 ggatgttgac gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ctgaaggggc
     7021 aaatatgccg accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc
     7081 accgggtaaa gcggctaatg ctggcggtgt agcaacgtcg ggcctggaaa tggcacaaaa
     7141 cgctgcgcgc ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct
     7201 ggatatccac catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca
     7261 gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat
     7321 ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc tacagatggg cacaattcat
     7381 tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt
     7441 ttatgagtat ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt
     7501 cttactgtag tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc
     7561 cgttaacttt ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt
     7621 ttgataatct tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata
     7681 atgccaggcc agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat
     7741 catccatgat aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt
     7801 agcgcaatca gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac
     7861 ctgctctggg gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag
     7921 aaaatgctcc attaaggcac aataactttc gctatcttcg ataccccatt gattctctaa
     7981 agactcgcgt cttttactga tgatatcgat cgagtcaaaa ggaagcacat gatattggaa
     8041 ggtatctttg ccaggttcag gctttcgcgg ccagaactct agcgttgcag accattgctt
     8101 atgatagaat cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg
     8161 ctcaatacct ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg
     8221 aacaataagc cccatcataa ttaatagata aagaaaagag catcccgcgg taatcaggcc
     8281 tctttcattc aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca
     8341 tgttaaataa aacccccact tacaaagcag catggcctta ttttctttaa tcacccgttc
     8401 aaaattacta ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt
     8461 ttgtaataaa acttttttca tcacattccc tgttattaca tactttagag gcgataattt
     8521 tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg
     8581 actcgaatag cacgaaagat tcgctcgctt acgctatcgc cccgcttccg gcttcatctg
     8641 ctggcggact ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc
     8701 caccactgcc cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat
     8761 caataagctg atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct
     8821 tttcgctgat ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca
     8881 tttctggcag cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt
     8941 ttttggtcag aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg
     9001 tccccagacc atcggtcgca cgaaggacct ttttcagatc tttgtcctgc acaaagcgcg
     9061 cgatcccggt catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt
     9121 gccgctctac cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca
     9181 atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc taacagcgtg cgccagcctg
     9241 cttcagcaag aaaacgggct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa
     9301 cacacttgcg gaacaccgca tccgggcaga actgcatcag gtattgacgg gcaatcaggt
     9361 tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga actccgtgcg gtcgggatga
     9421 tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta
     9481 ccactggctg cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc
     9541 gtccggcaaa atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct
     9601 tgtgcgtttc gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc
     9661 gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt
     9721 cccgtttatc gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat
     9781 gctccgccag tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt
     9841 cgctcggttg ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgtgctttga
     9901 cttcaaagaa gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc
     9961 cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac
    10021 cgagaatggt atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg
    10081 ccagcgcaga aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc
    10141 gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt
    10201 ccggtgccag ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg
    10261 ggtccccggc gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt
    10321 gtttggtcac ggagggtcgt ggctgtaatt gccacttttc cgggacaatc ggcaaatccg
    10381 caagattcca gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt
    10441 gaccgataca ccaagtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc
    10501 ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa
    10561 acaaccgcat tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt
    10621 tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct
    10681 gcttccggca tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg
    10741 cgcacttcac gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg
    10801 ccgccaggta ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct
    10861 tcatagtcga cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc
    10921 agaccaaagc ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg
    10981 cctgcgatgt tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac
    11041 agtgattttt tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt
    11101 ttgcatccgg cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt
    11161 accgccagac caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata
    11221 cccgcctgac ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa
    11281 agtttgttaa tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg
    11341 aagatatcgc tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg
    11401 atcggcataa agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc
    11461 gccgcatcgt cgcgggtttc attacccaca agcaaattcg gatcaacaaa cttcgcctgc
    11521 tcactgtgca ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg
    11581 tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat
    11641 ccgggtatgg taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc
    11701 tttgctcaca atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg
    11761 ggtccgggac gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac
    11821 cgacaatttt atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta
    11881 atgcgccact gcgccagatg ccgccaaatc cctgggcaac ggctgccatt tgcatcgcca
    11941 tgaccgcgca tccggcagac atttcctgtt cccatcgcgg gactttatga ttttctttgc
    12001 atttcgccac caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga
    12061 tagctttatc atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct
    12121 cgcccccttc cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg
    12181 cacgcatacc cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca
    12241 ggcgggaggc gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg
    12301 tcgtgatatt tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa
    12361 ttcctgctga caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca
    12421 ggtgtgacct taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt
    12481 ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta
    12541 ttttcctcgt actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag
    12601 aaacggccag tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaaccag
    12661 acagttctca gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc
    12721 tgcaggaaaa ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca
    12781 atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc
    12841 agtacatcgg caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg
    12901 gcgagaacta cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt
    12961 caccgcaagg cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt
    13021 tgctggataa gctgaaagtt tccccccatg tgttccgcgt gggtacgtat aaatctgccg
    13081 ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga
    13141 ttggtgagct gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc
    13201 agcaggtatt ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata
    13261 ccgcgaaata tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg
    13321 aaaaagctct gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca
    13381 gttattacga ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct
    13441 tcgctaatgg cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca
    13501 ctgcggcaca aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg
    13561 ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag
    13621 cccgggcagc gggtaagtct gtggttgtat cgatgggcgg catggcggca tctggtggtt
    13681 actggatttc cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta
    13741 tcggtatctt cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata
    13801 ctgatggtgt ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg
    13861 aagcgcagca gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg
    13921 ttgctgatgc gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct
    13981 ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg
    14041 cggttgccaa agcagcagag ctcgcaaaag tgaaacagtg gcatctggaa tactacgttg
    14101 atgaaccgac cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt
    14161 tgccagatgc gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta
    14221 aaagtgaaag cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc
    14281 tgacctgcgc caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat
    14341 tattttttac ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat
    14401 agtatcaggt gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat
    14461 atcaatatca tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg
    14521 cagcgttccg agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg
    14581 atgccggaat tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg
    14641 atggattctt ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg
    14701 cactatgacg actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc
    14761 gcctctgcgc tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca
    14821 caaatcccgc tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac
    14881 gttgcggcga attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc
    14941 ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt
    15001 cctccgttac tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac
    15061 ggtgaagggg aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt
    15121 tatccaggga tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg
    15181 attctgcgct cctatggcgt gggtaatgcg tcacaaaaca aagccttcct gcaggaatta
    15241 caagaagcca gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa
    15301 gtgaatatga gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt
    15361 gcagatatga ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg
    15421 gatactgaaa ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat
    15481 gattaaggag actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt
    15541 ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt acggtggagg ttgctaaccg
    15601 ccttattgac tggtgccagt cgcgcggaga agcggttatc gccagccagg actggcatcc
    15661 ggcgaatcac ggcagctttg ccagccagca cggcgtagag ccttatacgc caggccaact
    15721 cgacggcctg cggcaaactt tctggcctga acactgtgtg cagaacagtg aaggcgcaca
    15781 attacatccg ttactgaatc aaaaagagat cgcagcggtg ttccataaag gcgaaaatcc
    15841 tttagttgac agttacagtg ccttttttga taacggacgt cggcagaaaa ccgcgcttga
    15901 tgactggttg cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta
    15961 ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac
    16021 cgatggctgt cgtggcgtga atatccagcc ccaggacagt gcacacgcgt ttatggagat
    16081 gtcagcagct ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaatt
    16141 ttacgctggc ctacaattct gtactggcat tgtaggccaa ataaaacacg tcagtggtac
    16201 atctggcaat tgatgccatc aacgaaagat ttaattcacc tcagaaatct cttccagcga
    16261 caccttccgc gtttcgatgc caaaaatgga gagaaccagc gcacagagca ctaacatgac
    16321 accaagcacc ataaacaccg tgatcgaccc ataatgtgtt aataatgccg caacgccata
    16381 gggcgtgaaa actgcgacga tccgtccgac ggcattaacg aaacccgaac cgcgcaggcg
    16441 taaatgcgtt ggccaaagct ccgggatata aaccgccgac gcgaagcaaa catacatgta
    16501 taaaaagaag atcatcacca gaccatagat taaaatcgcc cactctgtag tctgacttga
    16561 atagatatag cctaacacgg caataataat aagtaaggcg gagccaaata atcgacgagg
    16621 aaaatgatca ataattaatg ccgcaataaa tattcctacc ggagcaccaa tcataataac
    16681 agcggtcatt aatattgatt tatcgacatc aatgccggag ttaacaaata tggtcggtat
    16741 ccatacggta atggtataaa gcgaaatgtt cattgcaatt aaaacagtta tcgcaactaa
    16801 tgtacgtcgt aacatttccc ctttaaacag gagccagaag gtacccttga ttaccttaac
    16861 cttgctgttg ctctggttca aagtaagctg gggtaaacga atactcttct ccctttcaat
    16921 ttgctgctct acttcacgaa gttggctttc tgcaccggcg atttgccctt tccctgccag
    16981 ccatcgtggc gactcgataa agtatttacc tgagagaaac caggctaaca gtatgccaat
    17041 accacccaga agaaacatta ttcgccaact aaaaaaagcg ataaccacca cgcctatcgc
    17101 cgcagacagc atgggcgacc agttaccaac aaatgagagc cgcgcggacc attttccacg
    17161 caccgtcgcg gggataaact ccgtaaatga ggcatagcca accataatca gcgcccccat
    17221 tcctgttccc atcaggaagc gaaagaaaat gagccagtac atatcaggga caaaagcggc
    17281 ccctgttgca gcaataccga cgatgagaag atttatgcga aacgccctgc gccgcccaaa
    17341 gtagtcacca ataaacccac cagtaagtga gccgatgaaa taaccaaaca ttaatgccga
    17401 ggtaaaggcg gcattgagga aattatttga ccatccattg cttaccagct ttgctaagac
    17461 gacattacct gagtaactca aaaaccccgt taataacaga ctaaagctta ttataccaaa
    17521 tatacgataa tgaaaccggg ccaaaggcaa gcggtccagt cttgcgccaa tttgatcata
    17581 ttgttccatt gggtgcctcg gatttgctaa aaaaggtagc aattgcggca aattcacttg
    17641 ccgcatcttt atcatcattt tatttccagg cgcaggaaca aataatattg acaacgtgtg
    17701 cttaaattac cacaccagct ggatattatt gtctttgcaa aatgctatcc actctgcacc
    17761 cggtgattta tcagttatta tataattaat atgactaaag tcagctaact ggacaaaagc
    17821 tttacgatca aacttagagt gatcaaccaa taacgcaact tctgtcgcct gacggatcat
    17881 tgtctttttg atttcagctt ccgcttcatt agagtccagc gcgccgctgt taatatcaag
    17941 acctttacaa ctcattacca tgatatcgac atggtagcgg ctgatgatct ctttagtaat
    18001 tcttccttgc agggaaagtg tatttttgtt tagttcccca cccgttgaaa cgactttaat
    18061 ttctgactga gccagaacat gaatcgcttc cgccgagttg gttagcaacg tcaggtcact
    18121 acggtcctgt aacaatttga gcaattccat tacggtacta ctggaatcgg ctgccatggt
    18181 ggttttattg tcgataaagg gtagtgcctt gcgtgcaata agctgcttct cttcataaaa
    18241 cgatgaagcg cgcttataaa aatggatatt ctccgtcaac atcgctgtat ttaaaacagc
    18301 accaccatag gttctggtca aaaagccttc atcttccagc ttctcaagat cgcggcgaat
    18361 ggtttcttcg gttacctgaa aaatcccact caaatttgag actgtcacct ttttatcgtt
    18421 ggcaaccatt tgcttaattg cctgaatcct gtcttttgcc gccacgatta cacccctgta
    18481 tctttttaca tcacattagc gcgattatcg cataaccgat gtttactttc aaaataacct
    18541 atttgaatca cagattttca tcacagtttt cacagaaaca gaggtgaatc gtgttgagta
    18601 tttaacgctc cagggcctct gccatttccc tcatcaatgt tgcgtctgca tccgataagt
    18661 tgatattcag tgccgcgaca ttttcgcgta cctgttccgg tgcagtagcc ccactaagaa
    18721 tggagattaa atcactctgt tttaatatcc acgccagtgc cagagtggga attgtgcact
    18781 gataacgagc acaaagtggc tgccactgtt caagcatatc aatcaatttc agcatgtttt
    18841 cacgctggaa ccagacttta tttgcccgag cgccgcccgg aacgtaatca cgagtgatgg
    18901 tgccggtcaa caatccctgc tctagcgggg aataaacctg aaccacaatg ccattatcac
    18961 gacatagcgg cagcagttcg ttttccattg cccggtcgag aatactgtat ttcgcctgaa
    19021 taatatccag ttcaccatat tgcagatact cgcggatatg gtcagcatcg acgttagcag
    19081 cgcctatagc gcgaattttc ccttcagcct ttaactcatt aagcactgcg acagtttcag
    19141 ctatcggcgt aaaaaatggc ggcaccgact gccagtgcgt catatagata tcgatgtaat
    19201 caatacccag acgttgcaag ctggcttcta cctcttcgcg gatagattcc ggggaaaggt
    19261 ttttatacaa ctgccgatcg ccaactttgt tgaataaact tccttttcgt tcccagacaa
    19321 tgccgcattt ggtttctact acaacctgtt cacggggcag tttttttaac gcctgaccga
    19381 cgataacttc actattgcca aagttatatc ctggcgcagt atcaatcaga ttaatgccac
    19441 aacgatgggc ttcaagaatc gtatcaatac atatttgccg atcgagatcg ccattccatg
    19501 caggaccgcc gccaatggcc catgtcccca accccattcg cgaaagcgtt atatccgttg
    19561 tgcctaaagg tatctttttc atttgccttc attatccttc gtattcttcc agcaattgtt
    19621 cgaccagctt tctgtttttt acgccggtgg tggcaccgac gcttagaacc gagatagccg
    19681 ccgttgcatt ggcaaagcgt gcgcattcac gcagattttt gccttctaac agtgccgcaa
    19741 taaaacctga agcaaagtta tcgcccgcgc caatggtgtc gatggcggtt attcctgcga
    19801 ccgccggcac cttcatcgtc atgtcaccac gcttgataaa gcagccgtct ttacccgttt
    19861 taatcaccac cgtttttacg ccgcacgcaa gaaagcagtc agcaatttca tccagtgtct
    19921 ctttccctgt gagtaatttt gcctcggcaa aatttggaaa cagataatcg acataactca
    19981 atgcttcgca aatatcatcc agcgtttcat tcaaccgcgg tttgatcata tcggcacaaa
    20041 tgatcatctg ccgggctttc gcttgcgtaa aaatttctgt tagcgcttta ccatccaata
    20101 gaggactgtt gaaaatactg gccagtgata ataatttcgc ctgaggaaac cgcgcaaaat
    20161 caacatcgtc aatatttaat ttccacaaac tgccgttacg gttcgtgaca aacgtccgct
    20221 cgccatcctc cgtcaccagt ccaacgttaa tagatgtatc tatgctgacg tcctgtttca
    20281 ggctttgaat atcaatattc tctttgcgac aatggtcgag aataaattgc cctgaggcat
    20341 ctttaccaat gcggctcatt aatgcggtac gatgccccag acgagaaata attgttgctt
    20401 catttattgc atcgcccccg gtgctcattg cgattctttc aagaggataa gaatctacat
    20461 caaagatatt tttactgacc ggttgtaatg gaatatcaac aatagcggca cctatacaaa
    20521 taacgtcgag attatccata tcattattcc gctttgccat cagaaccgaa cagttcaatt
    20581 ttttccaatg cccgttcttt tacagcttta cgtacttcac gttctaaatg caggaaaggc
    20641 tgatcctggt tttctttcac tgctaccatt gcggcctgac agagctccgt atgaatgttg
    20701 attttcgcaa tacccaacga aattgcagtt ttaatgtcgg catcactgat ccccgatgcg
    20761 ccatgcaaaa ccagcggaac agaaacagca tcgcgtacgc gtttgacgac ctcaaagttc
    20821 aattgcggct ctgacgtata aaccccatgc tggttgccga tggcgacagc caatgagtca
    20881 cagcccgtac gttcaacaaa ttcagccgcc tggtcaggat cggtatagtg atagcccgcc
    20941 agcgcttcct cataaacggt ttcattaccg acatgcccta attccgcctc taccggaata
    21001 cccagcggat gaaagaaatc aacagcctct ttggttaaac gaatattttc ttcgaaatca
    21061 aatgcagaag catcacgcat taatgaattc ataccatgag tccaggcgtt atgaataatc
    21121 tccatactcc gaccatgatc ccaatgagtt attaccggca ccgttgcttt ttgtgccatt
    21181 gataccatca tgtgagagaa atcttcaaat gaggtgttac cgacaaaacc tgtaccaaaa
    21241 gaaataataa ccggggattt cgcttcttcg gctgcgtctt taacgcccat caacatttct
    21301 gcattccata cgttaaaatg ggcaattgca taatgtttat ttgtggcatc gttttcccaa
    21361 tatctgatat ctgcgagcat gttctgttct cccgtattaa ttaatcgcta attttaataa
    21421 cgcctttaat aatgtcgcgt ttgttgttaa ctgactcttc aaatgcctgt tgtacatccc
    21481 gataatcgta aatatgcgtc accatcgatt tcacatcgaa tcgccctgaa gaaatagctt
    21541 caatcgtgac cggataacga ttggcatagc ggaataccgt ctggatagtg acttcgcgat
    21601 tgattttgag gaaattgatt gccgaatcgc cgggtacagt accaacaatc ataattttac
    21661 cgccgcgcat taccagataa ggtgcctgtt taacggtgac cgcagaaccc gctgtttcga
    21721 aaacaatatc tgcgcccatg tcttcggtaa attgctgaca gcgtgcaata gtgtcctctt
    21781 ttgcgccatt aataaccact gtcgcaccaa gctgttccgc cattgccaga cgtttttcca
    21841 gtacatcaac gacggcaatt tccgttgcgc ccaggcattt gcacgcttgc aacgtcatca
    21901 aaccaataca acctgctccc agaataatta tcttcttacc cggtttaaca tctgccagca
    21961 tcgcggcatg catcccgact gcggcaggct ccaccagcgc cccttccatc gtgtccatat
    22021 tgtcgggcag tttgtaagta aagctctccg gatgacacag atagtgcgtt aatgcgccgc
    22081 ggtagttggg ttgtgtcgcc ataaaatcaa cgtccgggca gatgttatat ttgccttcca
    22141 gacagtaacg acagtgaccg caaggaacgc caggttcgat atttacccga tcccccggtt
    22201 taaatttgcg cacgcggctt cccacagcca caaccgtccc ggcgcattca tgacccaggc
    22261 caatttcttg atttgggtct ttaggcggaa taaacgggcc tgattcaaaa ccatgtacat
    22321 ctgaaccaca aataccgaca tattctactt taatcaaaac ttcatcttct ttaggcactg
    22381 gtatttctgc tgaaataatt ttcattgtgc ccggcacctg caatattgct tttgaatttt
    22441 tcattttgaa tccacccgta ttatttattg gctactgcat caatacttaa actttcaacg
    22501 gagacacctt tggtttcaat tccaatggtg gcgatagcaa ttgcgacaat aattgaaaca
    22561 gcccccagaa gaataaagac tcccgttaca ccataactac tgagcagcac tgcaacggca
    22621 taaggtgcgg caataccact gattcgccct accgcattcg ccagaccgga gccacggagt
    22681 ttggcctctg tcggccagat ttcagggaca tacactgccg aggcatagca aacgtacata
    22741 tagacgaaag taatcaggaa gaaaccaatc agcgttatga gcaacatact ggtttgcagt
    22801 gaatagatat atccgagcac cgcaatcagg attaatagcc ccacacccat tgttttacgc
    22861 ggaattttat ccatcaccag catggcaata aaaataccaa atggcgcgcc aaacatactc
    22921 atggtattta aaacaatcga gtcttttaaa ttaatcccct gggtcatgaa tattgttggc
    22981 aaccagttaa ttagtgtgta ctgcacaacg ttcatggtaa tcagcacaca agagcctaat
    23041 atcacgcgtt tcagtaatac tcctgtcagt aaggctgaat acggcaccgc ttgtggcgct
    23101 ttaccgtcat cagcaataac cacaggcggc aaaggtttac ccgtctggcg tatgacgcct
    23161 tcttctatac tccgcatgac tttctcggct tcctgatacc gtccgcgcga ttccagccag
    23221 cgcggggatt caggaaagta gcgccaggcc agcgccgtag cgataagcga caatattgca
    23281 ggaataagca gttgtacccg ccagttccac tctgcactaa tcagcggcgt gagtcccatc
    23341 gcaatcagtg agcagagcgg atatgaccag ttgccaataa aggaaacacg actggaccac
    23401 gttccgcgat ttctaccggg catatattcg gtgaaaccag caagcagtgt aaccagtaaa
    23461 gctcccagcc caacgcccat cacaaaacgg caggcaataa gaaaatccat attcggtgag
    23521 aaagcaccga caaccatcga ggcaatatga atggcctcat agaggatgaa cgcatttctg
    23581 cgaccggttt tgtcgccaat gatgccgccc accagcgcac cgagaaacat tccggcggtc
    23641 gtgattgctg agaatgtggc tgtggtggaa ttatctgtcc agcccaacgc tttcagctgc
    23701 gcgaggatca agccaccaac ggcattactc cagcagacaa gcaagccaaa cgcgacgatg
    23761 gcaaacattg acgaatgcca gcggcaatcc ggtaagcgat ccagccgtgc accacaatgc
    23821 ggttttgtta tctgttccat gcgataacgt tccttcagga gagtagttat tcatcaaagt
    23881 cgtaagtcat gatcactttg attgcggttt tatcgaccat cgcatcaaac ccttcgcgcc
    23941 attgcgacag gccgatacga tgcgtgatca tcggtttgac tttgatagcg ccgctggcta
    24001 atagccggat agcgttacgc catgaggtgg agtcataggc catatgccca atgatgcttt
    24061 tattccaggc ggtaatgtca ttaatcgaga aatcaagagg tttgaagccc attccaacgc
    24121 gtaccacttc cccattcggg cggagcattt cgatggcttg tttcagtgcg atattggcac
    24181 tggagcattc aatcaccagt cccagattat ctttgccaca aatttgctgg cagcgcgcca
    24241 ccacatcttc ggtagaacca tttactactg ccgtcgcccc cagttctttt gcaaccggga
    24301 agcggaccgc cacatcttct tgcagaccaa cgacgacgat atttaccgcc cccataatcc
    24361 gcgccatttg tacggaaaac agcccgagtg ggccagtgcc aatgacgaca acatcctgac
    24421 caggaaggaa tttcgattgc tgcgcgatgg atttataggc attacagata gggtcaagta
    24481 cggctgcgtc ctcataatca acaccatcag ggatttccca caacgcatga cgatgaattt
    24541 tgagaatttc accaggaacc agacaatatt tggaaaaacc accgccccag gtattgttat
    24601 ccagaccaag gtttactttt tctgtacaac acagaaaatc accctgttca caggccggac
    24661 aaacgccgca aacgtgaccg ctgttatccg acacgacgcg ttgccccact ttccagtctt
    24721 tgactttttc accaacctgc gcaatacaac ctgcgaactc atggccgcgg atagagttaa
    24781 actcatcaga accgctatcg acattgtagt gcttcatgtc tgcgccgcag attgccgcgg
    24841 ctttaatttc aatcactacg tcttccgggc cacaaatggg ttgtgggaca tgaatcatct
    24901 tgtagccgcc aaaggccttg ccaaaccgag ccagtgcttt cattgcgtca tcctctgtgt
    24961 tttgttttgc ttagatggcc atcatactta cccacatgat ttttgaggtc aacattaaat
    25021 ctgcataaca cagataatga cctgcgcttt acaacttaga tcacaattac gcagaaaatg
    25081 gctgggtctg ttacaggttg gtggaaggcg ggacgcaaaa agagcaaatt cgagagggat
    25141 taccggccaa ataggtctga gacttaagtt caggagaagt cattcaggcg tgaacgcgac
    25201 tgcaagagat cggactggga cttagccagg tcgggtcgag ataattgaga cgagtcgtgg
    25261 ggtcattgtt ggccatcccc ttcatagccg cggcaaaaag gggatgaacc acttacttaa
    25321 acatggcaat aggctttgct gagataccaa aatcttcttt taactgctgt ttgctcttca
    25381 tgaccatctg accatttgtg tcaattgtca tatgctgtgc ttcggtatta tggcgggctt
    25441 gccacagcat caccagttgc aggcaattct ctttttgttc ctcggtcaac gcaacgccat
    25501 caggccattt ccccagttca acggcggtcg acaaacgctg gtatacctca ggcgtcatgc
    25561 tgttgataat gtcatcaaga ttcataattt ttccgctcct gtggaataag ttgctgaatc
    25621 gttttttcaa ccgttgattt cttcgccgtt ttcgccatca gtgaagcgta aagaagcaga
    25681 gttaacacaa taacgttcgc ctgttggttg cggcccgtcg ggaaagacat gccccagatg
    25741 ggcatcacag ttaccgcaac gaatttctat gcgctgcatt ccatgtgaca agtctttgat
    25801 ataacgaatg gattcttcac tcaccggttc gtagaaactg ggccagccac agccggaatc
    25861 atacttggtt tcggaatgaa acagcggggc atcgcagatc aaacagtgat atacgccgtc
    25921 acgcttgtta tgcagtaaac gacccgtaaa tggcggttct gtcccatgat tctgcgtcac
    25981 gtaaaactgc atctcggaca aatttttttt cagttcttct gccgaaggtt tattagccat
    26041 ttactcacat ctcactttaa tcgtgctcac attacgtgac tgattctaac aaaacattaa
    26101 caccaactgg caaaattttg tcctaaactt gatctcgacg aaatggctgc acctaaatcg
    26161 tgatgaaaat cacattttta tcgtaattgc cctttaaaat tcggggcgcc gaccccatgt
    26221 ggtctcaagc ccaaaggaag agtgaggcga gtcagtcgcg taatgcttag gcacaggatt
    26281 gatttgtcgc aatgattgac acgattccgc ttgacgctgc gtaaggtttt tgtaatttta
    26341 caggcaacct tttattcact aacaaatagc tggtggaata tatgactatc aaagtaggta
    26401 tcaacggttt tggccgtatc ggtcgcattg ttttccgtgc tgctcagaaa cgttctgaca
    26461 tcgagatcgt tgcaatcaac gacctgttag acgctgatta catggcatac atgctgaaat
    26521 atgactccac tcacggccgt ttcgacggta ccgttgaagt gaaagacggt catctgatcg
    26581 ttaacggtaa aaaaatccgt gttaccgctg aacgtgatcc ggctaacctg aaatgggacg
    26641 aagtgggtgt tgacgttgtc gctgaagcaa ctggtctgtt cctgactgac gaaactgctc
    26701 gtaaacacat caccgctggt gcgaagaaag tggttatgac tggtccgtct aaagacaaca
    26761 ctccgatgtt cgttaaaggc gctaacttcg acaaatatgc tggccaggac atcgtttcca
    26821 acgcttcctg caccaccaac tgcctggctc cgctggctaa agttatcaac gataacttcg
    26881 gcatcatcga aggtctgatg accaccgttc acgctactac cgctactcag aaaaccgttg
    26941 atggcccgtc tcacaaagac tggcgcggcg gccgcggcgc ttcccagaac atcatcccgt
    27001 cctctaccgg tgctgctaaa gctgtaggta aagtactgcc agaactgaat ggcaaactga
    27061 ctggtatggc gttccgcgtt ccgaccccga acgtatccgt agttgacctg accgttcgtc
    27121 tggaaaaagc tgcaacttac gagcagatca aagctgccgt taaagctgct gctgaaggcg
    27181 aaatgaaagg cgttctgggc tacaccgaag atgacgtagt atctaccgat ttcaacggcg
    27241 aagtttgcac ttccgtgttc gatgctaaag ctggtatcgc tctgaacgac aacttcgtga
    27301 aactggtatc ctggtacgac aacgaaaccg gttactccaa caaagttctg gacctgatcg
    27361 ctcacatctc caaataagtt gagatgacac tgtgatctaa aaagagcgac ttcggtcgct
    27421 ctttttttta cctgataaaa tgaagttaaa ggactgcatc atgattaaga aaatttttgc
    27481 ccttccggtc atcgaacaaa tctcccctgt cctctcccgt cgtaaactgg atgaactgga
    27541 cctcattgtg gtcgatcatc cccaggtaaa agcctctttt gcattacagg gcgcacacct
    27601 tctctcgtgg aaacctgcgg gtgaagaaga agttctgtgg ttgagcaaca acacaccgtt
    27661 caaaaatggc gtcgctattc gcggtggcgt accggtttgc tggccgtggt ttggtccggc
    27721 ggcacaacaa ggtctgcctg cgcacggttt tgcccgcaac ctgccgtgga cgctgaaatc
    27781 acatcatgaa gatgctgatg gcgtagcgct gacttttgaa ttgacacaaa gcgaagagac
    27841 gaaaaaattc tggccgcacg actttacgct gttagcgcat ttccgcgtgg gtaaaacctg
    27901 tgaaatcgat cttgaatcac atggcgaatt tgaaaccacc tctgccctgc atacctactt
    27961 taacgtgggt gatatcgcta aggtaagcgt cagtgggctg ggcgatcgct tcattgataa
    28021 agtgaatgac gcgaaagaag atgtactgac cgacggtatt cagaccttcc ctgaccgtac
    28081 cgatcgcgtg tatctgaatc cgcaagattg cagcgtgatt aatgatgaag cgctgaatcg
    28141 tattatcgcc gtaggccacc agcatcatct gaacgttgtc ggctggaacc cgggaccggc
    28201 gctttcagtt agcatgggcg acatgccgga cgatggctac aagacctttg tttgtgtaga
    28261 aacggcttac gcttcagaaa cgcaaaaagt caccaaagag aaacctgcac atctggcgca
    28321 atccattcgc gttgccaaac gttaatttgc gttaatgttg tgtgccgggt gcgatgcatc
    28381 cggcacgtat caacatcaca ccatatccag cgcagttttt ccttttggtg ccggatatgc
    28441 cttatccagc atagctaatt ccgctgacga aagttcgacc tcaagcgcag ctgcattttg
    28501 ttggacatgt gcaatttcgt tgactaccgc gttttttaac agtccattgc gcaaccgccc
    28561 ggcctgggct aatggactgt aagccatcac cggcatctgc tgttgctggc accaggggag
    28621 tagatcgtac tcaatccctc gtgaaccgag atggtaaagc acctgattag tggcacactg
    28681 atttcccccc ggcagctgcc agagttcctg catatcggca taaccaaggt tagaaacgcc
    28741 ccagcgacgg atttttccct gggcgatcaa tttttccatc gcagcaacag tctcttcaaa
    28801 agcgaaactg ccagaccagt gtaataagta gagatcgaga taatcagtat tgagacggcg
    28861 taaactggct tcgcatgcat ttatcgcttt ttgcccgcca gcattccacg gatagacttt
    28921 agagacgaga aagaccttct ctcgcagacc ggttaatgct tccccaacca ccttttcggc
    28981 accgccatcg gcatacattt cggcggtatc aatgagggtt aaaccgagtt caatgcctgc
    29041 gcgtagtgca gcaacttctg ttttgcgctg actggcatct tcgcccatat accatgttcc
    29101 ctgccctatg gctggcagtg agacatcgcc actaaattga atcatttttt gttgcattgt
    29161 ttcctcccag gtattgcacc accgtaatgc aaaacagggc gtgacgccct gtttttatgc
    29221 acaaaatgcc ctggaaagat gcattatcag aatttgtagg tgatcccggt agaaatcagg
    29281 ccagtccagg atttatccac catcgggctg tcagtaactt catcagacag acgggtgtag
    29341 cgcgcggtac cgtaaacact ccagtcgccg aggaagttgt agctggcgct cagctcaagg
    29401 taagggctcc agctgtcatt cgggttatag ccacgcagac cgctgcgagc ggactctttg
    29461 cgcgatacgc cataatagta ttcgttctgg ttttcgctgt tccactgcac accaataccc
    29521 ggagtcacgg tcaggccacc gttggtgtaa cgatacaacc aggccatatc ccagacaatg
    29581 ccgttgctgt tatccagggt atcgccagcc agggtggtac gcaggtaacc gtactgggta
    29641 aagtgagcat aagacagacc agccatcatg gtgctcttac ggtcatccag gtgacgcatt
    29701 tggtgatcgc cactgtcttt cgctttgaag taaagcggcg accagtaagc ggtaattgaa
    29761 agtttatccg ttgcgtcatt ccacaggtag taaccaccac ctaagccacg gaaccagaag
    29821 ttatcgcctt catagttgat taccggtact gggtaaacat cggtatcgta atctttatat
    29881 gggtgctcaa cgacacctac gcctgcgccc agggaaaatt taccttcagc gtgcgctacg
    29941 cctgcagacg ttgcgataag cactccaagt gccagaagtt tgagtttggt cacaattaat
    30001 cattccttaa acaaatgttt agcgggcgac aaagtttacc cgtcaataca tcgaaaccca
    30061 acctttttac gttttcattt tttaaagtaa ctgtttaatt ttcctgccgc ggatgacacc
    30121 gcgcttacag ccaaatgaat ttagcgttac tggcgagcct ggtctttaca ttaattatgc
    30181 aaaatttatg gatgagttgt tgatatgcca ttgaaattaa gaaagccgtg caggcaagtt
    30241 ttccatttgc catctacgct taattttgaa ggtgtatcac cgggcacgtt gttctcatcg
    30301 tcgataaaat ggcatgagag ttgctgtgtt ttagcaagag acgtcgttca gtttacctct
    30361 tccgggagcc tctactattc atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac
    30421 ggcataccat gaatatattc gatcactatc gccagcgata tgaagctgcc aaggacgaag
    30481 agttcacact gcaggagttt cttaccactt gtcggcaaga tcgcagtgct tatgccaacg
    30541 cggctgagcg gctattgatg gctatcggtg agcctgtcat ggtcgataca gcccaggaac
    30601 ccagactttc tcgactcttt tctaaccggg tcattgcacg ttatccggca tttgaagagt
    30661 tttacggcat ggaagacgcg attgaacaga ttgtctctta tctgaaacac gcggctcagg
    30721 ggctggaaga gaagaaacaa atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat
    30781 cgcttgctga gcgactgaaa tcattaatgc agctcgtacc gatttatgta ttgagcgcca
    30841 acggtgagcg tagcccggtc aacgatcatc cgttctgtct tttcaatccg caggaagatg
    30901 ctcagattct ggaaaaagag tatggcattc ctcgccgtta tctcggcacc atcatgtcgc
    30961 catgggcggc aaaacgcctg catgaatttg gtggcgatat cactaagttc cgggtagtga
    31021 aggtctggcc gtcaattctg caacaaattg ctatcgccaa aacggaaccc ggtgatgaga
    31081 acaaccagga catctccgcg ctggttggga aagtcgatat tcgtaaactc gaacactacg
    31141 cgcagaatga cccggacgcc tacggctatt ccggtgcgct gtgccgcgcc aatcagggga
    31201 tcatggaatt cgttgagatg tttaaagcac cgataaaagt gctgcatccc ttgttaaccg
    31261 ccactcagga aggtaactac aacgggacgg aaggtatctc cgccctgccg ttcaacggga
    31321 ttattctcgc ccactcgaac gagtccgaat gggtcacttt ccgtaataac aaaaacaacg
    31381 aagccttcct cgaccgtgtt tacatcgtga aggtgccgta ttgcttgcgc atttccgaag
    31441 agatcaaaat ctacgagaaa ttgcttaatc acagtgaatt gactcacgcc ccatgcgccc
    31501 ctggcacgct cgaaacactg tcacgttttt ccattctttc gcgcctgaaa gagccagaaa
    31561 actccagcat ttattcaaag atgcgggttt atgatggcga aagtctgaaa gacaccgatc
    31621 cgaaagccaa gtcgtatcag gaatatcgtg actacgccgg tgtcgatgaa gggatgaacg
    31681 gtctgtcgac gcgttttgcg tttaagatcc tctcccgcgt gttcaacttc gatcatgtag
    31741 aagtggcggc aaacccggtc catctgttct acgtcctgga acagcagatc gagcgcgagc
    31801 agttcccgca agagcaggca gaacgctatc tggagttcct gaaaggttat ctgatcccga
    31861 aatatgccga gtttatcggc aaagagatcc agacggccta ccttgaatcc tattccgaat
    31921 atgggcaaaa cattttcgac cgttatgtta cctacgcgga tttctggatt caggatcagg
    31981 agtatcgcga tccggatacc gggcagctgt ttgaccgcga gtctcttaac gccgagctgg
    32041 agaaaatcga gaaaccggcg gggatcagta atccaaaaga tttccgcaac gagattgtta
    32101 acttcgtact gcgcgccaga gcgaataaca gcggacgcaa tccgaactgg accagctatg
    32161 aaaaactgcg cacggttatc gagaagaaaa tgttctccaa taccgaggag ctgttgccgg
    32221 ttatctcgtt taacgccaaa acgtcaaccg acgagcagaa gaaacacgac gactttgtcg
    32281 accgtatgat ggaaaaag
//
LOCUS       DADLSE010000035        31553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLSE010000035 DADLSE010000000
VERSION     DADLSE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.035"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..170)
                     /locus_tag="JN692_001592"
     CDS             complement(<1..170)
                     /locus_tag="JN692_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7263931.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTV"
     gene            383..1846
                     /gene="bepA"
                     /locus_tag="JN692_001593"
     CDS             383..1846
                     /gene="bepA"
                     /locus_tag="JN692_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7263932.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKSYTKM"
     gene            1867..2226
                     /gene="arsC"
                     /locus_tag="JN692_001594"
     CDS             1867..2226
                     /gene="arsC"
                     /locus_tag="JN692_001594"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7263933.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2359..3105)
                     /gene="hda"
                     /locus_tag="JN692_001595"
     CDS             complement(2359..3105)
                     /gene="hda"
                     /locus_tag="JN692_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7263934.1"
                     /translation="MVNFSRFCEILVEVSLNTSAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3155..4444)
                     /gene="uraA"
                     /locus_tag="JN692_001596"
     CDS             complement(3155..4444)
                     /gene="uraA"
                     /locus_tag="JN692_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7263935.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(4530..5156)
                     /gene="upp"
                     /locus_tag="JN692_001597"
     CDS             complement(4530..5156)
                     /gene="upp"
                     /locus_tag="JN692_001597"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7263936.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5480..6517
                     /gene="purM"
                     /locus_tag="JN692_001598"
     CDS             5480..6517
                     /gene="purM"
                     /locus_tag="JN692_001598"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7263937.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKCHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6517..7155
                     /gene="purN"
                     /locus_tag="JN692_001599"
     CDS             6517..7155
                     /gene="purN"
                     /locus_tag="JN692_001599"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7263938.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7327..9393
                     /gene="ppk1"
                     /locus_tag="JN692_001600"
     CDS             7327..9393
                     /gene="ppk1"
                     /locus_tag="JN692_001600"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7263939.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9398..10939
                     /gene="ppx"
                     /locus_tag="JN692_001601"
     CDS             9398..10939
                     /gene="ppx"
                     /locus_tag="JN692_001601"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7263940.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEDAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10978..13221)
                     /gene="pdeF"
                     /locus_tag="JN692_001602"
     CDS             complement(10978..13221)
                     /gene="pdeF"
                     /locus_tag="JN692_001602"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7263941.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSLIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(13403..13555)
                     /locus_tag="JN692_001603"
     CDS             complement(13403..13555)
                     /locus_tag="JN692_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263942.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            13573..13764
                     /locus_tag="JN692_001604"
     CDS             13573..13764
                     /locus_tag="JN692_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7263943.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            14075..14593
                     /locus_tag="JN692_001605"
     CDS             14075..14593
                     /locus_tag="JN692_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7263944.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            14609..15148
                     /locus_tag="JN692_001606"
     CDS             14609..15148
                     /locus_tag="JN692_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263945.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15243..16820)
                     /gene="guaA"
                     /locus_tag="JN692_001607"
     CDS             complement(15243..16820)
                     /gene="guaA"
                     /locus_tag="JN692_001607"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7263946.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16889..18355)
                     /gene="guaB"
                     /locus_tag="JN692_001608"
     CDS             complement(16889..18355)
                     /gene="guaB"
                     /locus_tag="JN692_001608"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7263947.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            18517..19887
                     /gene="xseA"
                     /locus_tag="JN692_001609"
     CDS             18517..19887
                     /gene="xseA"
                     /locus_tag="JN692_001609"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7263948.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(19884..20099)
                     /locus_tag="JN692_001610"
     CDS             complement(19884..20099)
                     /locus_tag="JN692_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7263949.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(20169..21641)
                     /gene="der"
                     /locus_tag="JN692_001611"
     CDS             complement(20169..21641)
                     /gene="der"
                     /locus_tag="JN692_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7263950.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(21759..22937)
                     /gene="bamB"
                     /locus_tag="JN692_001612"
     CDS             complement(21759..22937)
                     /gene="bamB"
                     /locus_tag="JN692_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7263951.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(22948..23568)
                     /locus_tag="JN692_001613"
     CDS             complement(22948..23568)
                     /locus_tag="JN692_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7263952.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(23586..24860)
                     /gene="hisS"
                     /locus_tag="JN692_001614"
     CDS             complement(23586..24860)
                     /gene="hisS"
                     /locus_tag="JN692_001614"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7263953.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKQLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(24971..26089)
                     /gene="ispG"
                     /locus_tag="JN692_001615"
                     /gene_synonym="gcpE"
     CDS             complement(24971..26089)
                     /gene="ispG"
                     /locus_tag="JN692_001615"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7263954.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(26116..27129)
                     /gene="rodZ"
                     /locus_tag="JN692_001616"
     CDS             complement(26116..27129)
                     /gene="rodZ"
                     /locus_tag="JN692_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7263955.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(27414..28568)
                     /gene="trmG/rlmN"
                     /locus_tag="JN692_001617"
     CDS             complement(27414..28568)
                     /gene="trmG/rlmN"
                     /locus_tag="JN692_001617"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7263956.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(28718..29149)
                     /gene="ndk"
                     /locus_tag="JN692_001618"
     CDS             complement(28718..29149)
                     /gene="ndk"
                     /locus_tag="JN692_001618"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7263957.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(29298..31421)
                     /gene="pbpC"
                     /locus_tag="JN692_001619"
                     /pseudo
     CDS             complement(29298..31421)
                     /gene="pbpC"
                     /locus_tag="JN692_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            complement(31423..>31553)
                     /locus_tag="JN692_001620"
     CDS             complement(31423..>31553)
                     /locus_tag="JN692_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7263958.1"
                     /translation="YYTRKMSQGKRHNQVLIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         7950 a         8383 c         7800 g         7420 t
ORIGIN      
        1 acggttggcc attccagcaa ataagccagc acaatagcca ccagcaacgg agcaagcagg
       61 ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa ggcaatcgct
      121 tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat aaagaccttc
      181 cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac aaaaaagact
      241 tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta atcttcgccg
      301 ttacactcaa aggcggcgag gtgggaacga tatttcacag tatcggtcaa atgactatct
      361 tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg ttgcaaccct
      421 cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg cagacacctt
      481 gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa tgcagatggg
      541 cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc cgctgttaac
      601 gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg ttaagacacc
      661 gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct ttggcggcaa
      721 cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac tggcttcagt
      781 tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga tggaagatca
      841 acaacgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac tggcgatggc
      901 cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc gccaggggat
      961 gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc aggtgctgca
     1021 acgctcggga ttcgatccac aggcgatgcc gaccttcctg gaaaaattgc tcgatcaggc
     1081 gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg aaagtcgtct
     1141 ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt cgtcggaaga
     1201 tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta accagctcac
     1261 cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag cggcgcaata
     1321 tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta aaacgctgca
     1381 accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta ccgatatcga
     1441 tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc gcgatttacg
     1501 caccaatccg gtgttgcagc tcaacctggc gaacgcttat ctgcaaggcg gtcaaccaca
     1561 agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca gcaacggctg
     1621 ggatttgctg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc tggctgcgcg
     1681 agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt tgagtagcgc
     1741 cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc gcatcgacca
     1801 gttgcgccag ctgcaggaac gctttaagtc ttataccaag atgtaattcg cattaaagga
     1861 agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa gagccgggaa
     1921 acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtgctcta ccttgagaca
     1981 cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa tagcgcccgt
     2041 gaattgatgc gccagaaaga ggatctttat aaagaactaa acctggctga cagttcgctt
     2101 agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga gcgcccgatt
     2161 gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact ggaaatcgtg
     2221 ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc ttgccacgtc
     2281 ttatccggcc tacgaatact atggattaac ttcgcgtagg tcaggcgttc gcgccgcatc
     2341 cgacaataaa caccttatct acaacttcag aatttctttc acaaacggaa tggtcagctt
     2401 acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac gtcataaata gcgtgcgcat
     2461 ttctctgtcg agccgcttca gcaagaaacg ccccacatct tccggcagtt caaaaccacg
     2521 caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca tcagaaagtg gctgcaattt
     2581 gtagatctgc ccccagtcga gacgcgacgc gagatccggt aatcccagat tcaactgccg
     2641 cggtggacga tcgccggtga tcaacaaccg tgttttgccc gattccagaa ttcgattgta
     2701 gagatcgaaa atcgccatct cccacaactc atcgcctgca atacactcaa tgttgtcgat
     2761 acagaccagc gacaaatgct ccataccgtc gagcacttcc ggaacaaacc aggtgcgttt
     2821 atccagcggg acatagccca ccgcatcgcc acgctgcgac aattccgcgc aagccgcgtg
     2881 cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga tagatgtaac cgctatgttc
     2941 ctgacgcagc acgttttgca gcgcggccag taaagaggag ttatcccccg gccagaaact
     3001 tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag agctgtgccg atgtgttcag
     3061 agatacctca accaggattt cacaaaatcg cgagaagttt accacagaat cccatgatgt
     3121 ttgaaccggg cagcaacact gcccggtcgg tacattattt gtctgttata tccgcgtctt
     3181 ctgcgtccag caccacttct tcagggcgca gcacactgat cagtttgaaa atcaaactta
     3241 agccgatacc gacgatggtc gccagcgcca taccttttaa ctccgccgcg ccgatgttta
     3301 ccttcgcgcc actgacgccg atgatcaaaa tcacggaagt caggatcagg ttctgtgctt
     3361 tgttgtaatc cactttcgat tcgatcaaaa cacgaatacc ggaagcacca atgacaccat
     3421 aaagcagcag cgaaacgccg cccatcaccg gcaatgggat catctggata gcggcagcca
     3481 gtttaccgac gcaggaaagc aggatagcga aaatcgccgc cccgccgata acccaggtac
     3541 tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc gtaagtggta tttggcgtag
     3601 agccaaagaa gccggagatc acggtcgaca ggccgttagc aaacatcgaa cggtgcagac
     3661 ctggatcgcg cagcagatct tttttgacga tattagccgt tactaccagg tgccctacgt
     3721 gttcggcaat aaccactaac gccgctggca gaatagtcag aatggcaaac cactcgaagc
     3781 gcggcgtata gagggttggc agtgcaaacc agtgagcatt aataatcggc gtggtatcga
     3841 caattcccat tgcgaaagag agcgcgtacc ccaccagcac gccaattaaa atcgggataa
     3901 ttgccaggaa accacgaaac agcacggaac ctaaaaccgt gaccgccagg gtggtaatag
     3961 agatgatgat ggttttggag tttggcgttt gcccttcagc cgggagtaaa cccgccatac
     4021 cggcagctac gcccgccagc tccagaccga tgacggcaac gattgcgccc attgccgcag
     4081 gtggaaacag cacgtccaac cagccggtcc ccgctttctt cacgataaaa gaaaccaggc
     4141 agaacagcac gccgcacata ataaagccgc ccagcgcgac ttcataccct aacggcaaca
     4201 gtaacaatac cggtgaaata aaggcaaagc tggaaccaag ataagccgga attttccctt
     4261 tacagatgaa gagatacagc agcgttccaa taccgttaaa taacagtaca gtcgccgggt
     4321 taatatgaaa taagacgggc accaggacgg ttgcaccaaa catggcgaac aaatgttgca
     4381 aactaagcgg gattgtctgt aaaagtggcg gtctttcact caccccgata gcacggcgcg
     4441 tcatagtatt atcctctgta ttatgtgtta taggcgcttt actcaaaaaa aagccgactc
     4501 ttaaagtcgg ctttaattat ttttattctt tatttcgtac caaagatttt gtcaccggca
     4561 tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc cctggtcaat cgatgcagta
     4621 tacagttcga cgtccgggtg cgctttttcc agcgcagcga taccttctgg cgcagcgacc
     4681 agcaccagaa ctttgatgct gctgcagccc gcttttttca gcaggtcgat ggtcgcgata
     4741 acggaaccac cggttgccag cattgggtca acgatcagcg ccatacgctc atcgatgtta
     4801 gaaaccagtt tctggaagta cggtaccggc tccagcgttt cttcattacg gtacataccg
     4861 acaacgctga tgcgcgcgct cggaacgttt tccagcacac cgtccatcat accaagaccc
     4921 gcacgcagaa ttggcacaac ggtaattttc ttacctttga tctggtcgat ttctaccggg
     4981 ccgttccagc cttcgatagt tactttttcc gtttcgaggt cggcggtcgc ttcgtaagtc
     5041 agcaggctac ccacttcgga agcgagttcg cgaaagcgct tggtgctgat atcttgctca
     5101 cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga cttccacgat cttcatactc
     5161 tttctccttt gaggggcagc cacaaaaaaa tcgacggatt atacctcctt tcttcaaggc
     5221 ggcaatatta ttttcgttga ctttagtcaa aatgataaca gtttgagata aagttatttt
     5281 atattcaaat ggttatgaaa gaagattatt ccatccgaaa actaaccttt gccctggcac
     5341 aagtcttctt tcgccgcgcg cccggggaaa agacgtgcaa aaaggttgtg taaaacagtc
     5401 tcgcaaacgt ttgctttccc tgttagaatt gcgccgaatt ttatttttct accgcaagta
     5461 acgcgtgggg acccaagcag tgaccgataa aacctctctt agctacaaag atgccggtgt
     5521 tgatattgac gcgggtaatg ctctggttgg aagaatcaaa ggcgtagtga agaaaacgcg
     5581 tcgtccggaa gtgatgggcg gtctgggcgg cttcggtgca ctgtgtgcat tgccgcaaaa
     5641 atatcgtgaa cccgtgctgg tttccggcac tgacggcgta ggtaccaagc tgcgtctggc
     5701 aatggactta aaatgtcacg acaccattgg tattgatctg gtcgccatgt gcgttaatga
     5761 cctggtggtg caaggtgcgg aaccgctgtt tttcctcgac tattacgcaa ccggaaaact
     5821 ggatgttgat accgcttcag cggtgatcag cggcatcgcg gaaggttgtc tgcaatcggg
     5881 ctgttctctg gtgggtggcg aaacggcaga aatgccgggg atgtatcacg gtgaagatta
     5941 cgatgtcgcg ggtttctgcg tgggcgtggt agaaaaatca gaaatcatcg acggctctaa
     6001 agtcagcgac ggcgatgtgc tgattgcact cggttccagc ggtccgcact cgaacggcta
     6061 ttcgctggtg cgcaaaattc ttgaagtcag cggttgtgat ccgcaaacca ccgaacttga
     6121 tggtaagcca ttagccgatc atctgctggc accgacccgc atttacgtga agtcagtgct
     6181 ggagttgatt gaaaaggtcg atgtgcatgc cattgcgcac ctgaccggcg gcggcttctg
     6241 ggaaaacatt ccgcgcgtat tgccagataa tacccaggca gtgattgatg aatcttcctg
     6301 gcagtggccg gaagtgttca actggctgca aacggcaggt aacgttgagc gccatgaaat
     6361 gtatcgcacc ttcaactgcg gcgtcgggat gattatcgcc ctgcctgctc cggaagtgga
     6421 caaagccctc gccctgctaa atgccaacgg tgaaaacgcg tggaaaatcg gtatcatcaa
     6481 agcctctgat tccgaacaac gcgtggttat cgaataatga atattgtggt gcttatttcc
     6541 ggcaacggaa gtaatttaca ggcaattatt gacgcctgta aaaccaacaa aattaaaggc
     6601 accgtacggg cagttttcag caataaggcc gacgcgttcg gccttgaacg cgcccgccag
     6661 gcgggtattg caacgcatac gctcatcgcc agcgcgtttg acagtcgtga agcctatgac
     6721 cgggagttga ttcatgaaat cgacatgtac gcacccgatg tggtcgtgct ggctggtttt
     6781 atgcgcattc tcagcccggc gtttgtctcc cactatgccg ggcgtttgct gaacattcac
     6841 ccttctctgc tgccgaaata tcccggatta cacacccatc gtcaggcgct ggaaaatggc
     6901 gatgaagagc acggtacatc ggtgcatttc gtcaccgatg aactggacgg tggcccggtt
     6961 attttacagg cgaaagtccc ggtatttgct ggtgatacgg aagatgacat caccgcccgc
     7021 gtgcaaaccc aggaacacgc tatttatcca ctggtgatta gctggtttgc cgatggtcgt
     7081 ctgaaaatgc acgaaaacgc cgcgtggctg gatggtcaac gtctgccgcc gcagggctac
     7141 gctgccgacg agtaatgccc ccgtagttaa agcgccagct ctgccgctgg cgtttttcaa
     7201 ttcacctgta aatcgcaagc tccagcagtt tttttccccc ttttctggca tagttggaca
     7261 tctgccaata ttgctcgcca taatatccag gcagtgtccc gtgaataaaa cggagtaaaa
     7321 gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc tcagttggtt atcgttcaat
     7381 gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc tgattgaaag gatgcgtttc
     7441 ctggggatct attccaataa ccttgatgag ttctataaag tccgcttcgc tgaactgaag
     7501 cgacgcatca ttattagcga agaacaaggc tccaacgctc attcccgcca tttactgggc
     7561 aaaattcagt cccgggtgct gaaagccgat caggaattcg acggcctcta caacgagcta
     7621 ttgctggaga tggcgcgcaa ccagatcttc ctgattaatg aacgccagct ctccgtcaat
     7681 caacaaaact ggctgcgtca ttattttaag cagtatctgc gtcagcacat tacgccgatt
     7741 ttaatcaatc ctgacactga cttagtgcag ttcctgaaag atgattacac ctatctggcg
     7801 gtggaaatta tccgtagcga tagcatccgt tacgcgctgc tggagatccc atcagataaa
     7861 gtgccgcgct ttgtgaattt accgccagaa gcgccgcgtc gacgcaagcc gatgattctt
     7921 ctggataaca ttctgcgtta ctgccttgat gatattttca aaggcttctt tgattatgac
     7981 gcgctgaatg cctattcaat gaagatgacc cgcgatgccg aatacgattt agtgcatgag
     8041 atggaagcca gcctgatgga gttgatgtct tccagtctca agcagcgttt aactgctgag
     8101 ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc tggttgaagt gttacgcgaa
     8161 aaactgacta tttcccgcta cgactccatc gtccccggcg gtcgttatca taattttaaa
     8221 gactttatta atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc actgccgcgt
     8281 ttacgccata tttggtttga taaagcccag ttccgcaatg gttttgatgc cattcgcgaa
     8341 cgcgatgtgt tgctctatta tccttatcac acctttgagc atgtgctgga actgctgcgt
     8401 caggcttcgt tcgacccgag cgtactggcg attaaaatta acatttaccg cgtggcgaaa
     8461 gattcacgca tcatcgactc gatgatccac gccgcacata acggtaagaa agtcactgtg
     8521 gtggttgagt tacaggcgcg tttcgacgaa gaagccaaca ttcactgggc gaagcgcctg
     8581 accgaagcag gcgtgcacgt tatcttctct gcgccggggc tgaaaattca cgccaaactg
     8641 ttcctgattt cacgtaaaga aaacggtgaa gtggtccgtt acgcacacat cgggaccggg
     8701 aactttaacg aaaaaaccgc gcgtctttat actgactatt cgttgctgac cgcagatgcg
     8761 cgtatcacca acgaagtacg gcgggtattt aactttattg aaaacccata ccgcccggtg
     8821 acatttgatt atttaatggt gtcaccgcaa aactctcgcc gtctgttata tgaaatggta
     8881 gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagtg gtatcaccct gaagctaaat
     8941 aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc cggcgtaccg
     9001 gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca atctggaagg cattagcgac
     9061 aacattcgtg ccatcagtat tgttgaccgt taccttgaac atgaccgggt ttatattttt
     9121 gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg actggatgac gcgcaatatt
     9181 gattatcgta ttgaagtggc gacaccgctg ctcgatccgc gcctgaagca gcgggtgctg
     9241 gacatcatcg acatattgtt cagcgatacg gtcaaagcac gttatatcga taaagaactc
     9301 agtaatcgct acgttccccg cggcaatcgc cgcaaagtac gggcgcagtt ggcgatttac
     9361 gactacatca aatcactcga acaacctgaa taaccctatg ccaatacacg ataaatcccc
     9421 tcgtccgcag gagtttgctg cggtcgatct tggctcaaac agttttcaca tggtcatagc
     9481 ccgtgtggta gatggtgcca tgcagattat tggccgcctg aaacagcggg tgcatctggc
     9541 ggacggcctg gggccagata atatgttgag tgaagatgca atgacgcgcg gtttgaactg
     9601 tctgtcgctg tttgccgaac ggctacaagg gttttctcct gccagcgtct gtatagtcgg
     9661 cacccatacg ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg cggaaaaggt
     9721 cattccctac ccgattgaaa ttatttccgg taatgaagaa gcccgtctga tttttatggg
     9781 cgtggaacat acccaaccgg aaaaaggtcg caaactggtt attgatattg gcggcggatc
     9841 tacggaactg gtgattggtg aaaatttcga acctattctc gttgaaagcc gccggatggg
     9901 ttgtgtcagc tttgcccagc tttattttcc tggcggggtc atcaataaag agaattttca
     9961 gcgcgctcgc atggcggcag cacaaaaact ggaaacttta acctggcaat tccgtattca
    10021 gggctggaac gttgcaatgg gcgcttccgg taccataaaa gccgcccatg aagtgttaat
    10081 ggaaatgggc gagaaagacg ggataattac cccggaacgt ctggaaaaac tggtaaaaga
    10141 agttttacgt caccgtaatt tcgcatcgct gagtttaccg ggtctttccg aagagcggaa
    10201 aacagtcttc gttcctggac tggcgatttt atgtggtgtg tttgatgctt tagccatccg
    10261 tgaactgcgc ctttctgacg gggcgttacg cgaaggcgta ctgtatgaaa tggaaggacg
    10321 tttccgtcat caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc agtatcacat
    10381 agacagcgaa caggcccgac gagtgctgga taccactatg caaatgtacg aacagtggcg
    10441 ggaacagcaa ccgaagctgg cgcatccgca actggaggcg ctactgcgat gggccgccat
    10501 gctgcatgag gtcgggttga atatcaacca cagcggtttg catcgccact ccgcttatat
    10561 tctgcaaaac agtgacttgc cgggttttaa tcaggaacag cagctgatga tggcgacact
    10621 ggtgcgctat caccgtaaag cgattaagct cgacgatctg ccgcgcttta ccttgtttaa
    10681 gaagaaacag ttcctgccac tgatacagct attgcgcctt ggcgtattac taaacaatca
    10741 acgtcaggca accaccacac cgccaacatt gacactgatt actgatgaca gtcactggac
    10801 actgcgtttc ccgcatgact ggtttagtca gaatgcgctg gtactgcttg atctggaaaa
    10861 ggagcaagaa tactgggaag gcgtggctgg ctggcggttg aaaattgaag aagaaagtac
    10921 cccagaaatc gccgcttaat gctttgcggg ccgacgagaa atgtcggccc gcattattca
    10981 ggcactttcg cgaatgggtt cgatttcatt cagcgtatca attaacggtt gcggcttacc
    11041 aataagataa ccctgcatat aatcgatccc caaagagagc accgcctcgc ggatctcttc
    11101 gttttcaacg tactctgcca ctacccgcat tttcttcatt cgcgccagat ggcaaatcga
    11161 cgccacaatc tgataatcca gactatttga cacaatattg cggataaagc tgccgtcaat
    11221 tttaagcaga tcggcattca catttttaag ccgtgcatag ctggcgtagc cggtgccgaa
    11281 atcatcaatc gcaatctggc agcctaattc ctgaagatgc tgcaaggtga tttgcgcctg
    11341 cttaacattg gtcagagcat tactttcggt gacttcaaaa ataagttgcc acgcttcaat
    11401 ctgatattta gccagcaact gactgacttc aacaggaaaa cgagcctgac ataccgaggt
    11461 tggagacaga ttaatagcaa aacggtgagc gggcatcttc gctctgtttt cagccataaa
    11521 ttgcagcgta tgctcaatga cccacatgtc gatactcgac gataaaccaa attcgtgcgc
    11581 gaccggcaag aagctatcgg ggccgatcag ttcatcattc tcacctttca tgcgcagaag
    11641 aatttcatgg taaacatcac cacgcatacc ggtaatcggc tgggccatca ggaaaaaatg
    11701 gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg actttatctt tcaattcgcg
    11761 ttgcaaatac attgccccgc aacgctgcat attttccggg gcgttggtca cgatggaaag
    11821 ttcggcgacc gtatttagct ctcccagcag caggtagata tgattcactg gcgagcgcac
    11881 atagcagtaa ctgacgccaa tctgcggttg catcggcatc ccatcccaaa agaaacgaaa
    11941 ttgcttgaga tgactatcca gtgcggtaat gcgctcctgg tgcgattctg tattcagtcg
    12001 cagcgcgaga tcgttacccg aaagctgata aacatcttca cccggttcca gcaagggtga
    12061 cagccagtga gaaagttttt gcttgtattg aatccgcagc atgatgccat agttcttaac
    12121 cagcatttcc atgccaggga tgcgtaaata acaaagcgca gaccaagggg catcacgcaa
    12181 cgcgcgattc agggcgcgaa catttggcag atgaaccacc gggtccacat acgcaagccg
    12241 ctgaatgcgt ctgactaccg ctcgctgacg agttgccagt acagccatat aattgacaat
    12301 caaagagaat accagataac tggaggaggt tatggtcagc tgcgtggtat agccaggata
    12361 aatgggaatg taattttgat agctgtggat gctgatcatc aacacgaccg cccagagcag
    12421 cgaaatcagc ttataaccat agcgcattgc tccccacatc atcaggggca gcaataatga
    12481 caaggtataa ttggtgctaa aaattgtgct tttttcattt aacggcatgc ataacagcaa
    12541 caataaagca cctaatgcca gtagccagag cgcgaactct tttttggtga ctttggcatc
    12601 aacctgctgt tttaattgcg aataatagct acgtaaataa aatggatttc gcactacccg
    12661 aatgatgaag tagcacagcg ggacaccgat cagattaccc accagtaagg cctgataatt
    12721 gattaaagtc ccgaggttaa aaggcatgac accgaccaga ttttctctgc tcgccagtaa
    12781 tcctacaaat gcagcaaact ggaaaagtat cagaaacagc gttgcaggaa acactatctg
    12841 ccagaatata cgctgggaaa ttaaacgggt atcaccatgt gaaacgttgt tacgccgggg
    12901 agtaaagacc ctgtaaccgc cccaacagag aatgataatg ataataaaat tagccgttaa
    12961 tgagagcgtt tcgtaaaaac ccagctctgc atatttgcgg acaaaaatcc ctaaggtgat
    13021 ccctggcaat gccgcccagc tgaaaaacat catcatgctg atcatcagtg ccaaaggcag
    13081 ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa gtattaatgt gggcgaaaat
    13141 gggcaagatt aaagaaggca ggaacagcgg aagcccccac catttatcac gtatttttat
    13201 ataagttgca ttcagtttca tagatgctca gcagaatccc ccacatcctg aaggaggtgt
    13261 attcagacag gcatcccacc tgacttcgaa tgatgattat tcatcactat agagagcatt
    13321 gattctaagt gtcatatgaa agttccaatt gatatatatc aaacaaaata accctgatta
    13381 atgaattatt acgtttatca tgttaattca tcattattac atcatcattg taaataatta
    13441 aattaacttc cataacatta aaatatgtat ccactgacgc ttttttacat aacgaagaat
    13501 tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc cacagcgtgg gacatacttc
    13561 aaggaacctt ttgtgagtca ggcaactagt atgcgaaaac gacaccgatt taacagtcgc
    13621 atgacccgta tcgtactgct catcagcttt atcttcttct ttggccgttt tatctactcg
    13681 tccgtcggtg cctggcagca ccatcagagc aaaaaagaag ctcagcaatc cacactctcc
    13741 gtcgaatcac cggtacaacg ttagcggtta ccttctccac tttcacagaa cataacggca
    13801 cttcgctgtc ggatgctttt gctctttggg attatcaaag cggcagatat tctttcatct
    13861 taaattttac gtctttgtcc tgactgatgt ttatcctgtt tggctgcgaa ataaatataa
    13921 aattaatata tatgttgtaa tgatatattt ttataaatta ttccctgcgt gaattttaat
    13981 aaatttaatc tatcccttta tacgcaatac atttactttc ctcttttgat gatcttaaat
    14041 gtcttatttt tcgtaatgtg tataacaagg aatagtgatg aaatttaaaa aatgtcttct
    14101 gcctgtggca atgttagcgt cattcactct ggcaggatgc cagtcaaatg ctgacgatca
    14161 tgccgccgat gtttatcaaa ccgatcaact gaataccaaa caagaaacta aaaccgttaa
    14221 tattatttcc attcttcccg caaaagttgc cgtagacaac tcccaaaata aacggaacgc
    14281 acaagccttc ggcgcgctta ttggcgcagt cgctggcggt gttatcggcc acaacgtcgg
    14341 ttctggcagc aattccggaa cgacggcagg ggcagttggc ggcggagctg taggcgcggc
    14401 agcgggttct atggtgaatg ataaaacctt agtggaaggt gtttctttaa catataagga
    14461 aggcaccaaa gtgtatacct ccacccaggt gggtaaagag tgccagttta cgacaggttt
    14521 agccgttgtt attaccacaa cgtataacga aacgcgtatt cagccaaata ccaaatgtcc
    14581 tgaaaagagc taataatcag gaggagtcat gaagaaagtt tttctttgcg ccatcttagc
    14641 ctccttaagc tatccggcta tagcctcatc attgcaggat caactctctg ctgtcgcaga
    14701 agcggaacag caaggtaaaa atgaagagca aaggcagcat gacgaatggg tcgcggagcg
    14761 caacagggaa atccagcaag agaagcaacg tcgcgcaaat gcccaggccg ccgcgaacaa
    14821 aagagcggca acggcagcgg caaataagaa agctcgtcag gataaactgg acgccgaagc
    14881 cactgcggac aaaaaacgcg atcaaagtta tgaagatgag ctacgcagct tagagattca
    14941 gaaacaaaaa ctggcgctgg cgaaagaaga agcccgcgtt aagcgcgaaa acgaatttat
    15001 cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg gtgcaatctg aagctgacgc
    15061 aaacagaaat atgactgaag gcggtcgcga tctgatgaaa agcgtgggca aagcagaaga
    15121 gaacaaatcg gacagctggt ttaattaatc gatgttagta acttcaagcc tatgattctt
    15181 gaagataaaa aaccctctgt agtaacggag ggttttgttc attcatagtg cagggatcaa
    15241 aatcattccc actcaatggt agctggcggc ttgccgctga tgtcatacac cacgcgggaa
    15301 ataccgttca cttcattgat aatgcggttg gaaacgcgac cgaggaaatc gtacggcaga
    15361 tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag cacgcagaga gacaacccag
    15421 tcatacttac gaccatcgcc cattacgcca acggaacgta ctggcaggaa gacggtgaac
    15481 gcctggctga ctttgtcgta caggtccgct ttacgcagtt cttcaatgaa gatggcgtca
    15541 gcacggcgca gcaggtcaca gtactctttc ttcacttcac ccagaacacg aacgccaagg
    15601 cctggtcccg ggaacggatg acggtacagc atgtcgtacg gcaggcccag ctccagacca
    15661 atcttacgca cttcgtcttt gaacagctct ttcagcggtt caaccaggcc catcttcatc
    15721 tctttcggca ggccgcccac gttgtggtga gatttgatga cgtgtgcttt accggttgca
    15781 gaagccgcag attcgataac gtcagggtag atggtgccct gcgccagcca cttcacgtct
    15841 tccagtttca gcgcttcttc atcgaatact tcaacgaaaa cgcgcccgat gattttacgt
    15901 tttgcttccg gatcgttttc gccagccagc gctgacagga agcgatcttc tgccggtacg
    15961 tgaacaatgt taagaccaaa gtgatcgcca aacatatcca gaacctgctc tgcttcgttg
    16021 agacgcagca ggccgttgtc gacgaatacg caagtcaggt ttttaccgat agcacggtgc
    16081 agcagcattg cggttacgga ggaatccaca ccaccagaga ggccgaggat gactttatcg
    16141 tcgcctacct gctcgcggat gcgagctaca gcatcgtcga taattttcgc tggcgtccac
    16201 agggcttcac actggcagat atcacgcaca aaacgctcca gcatgcgcat accctggcgg
    16261 gtgtgagtca cttccgggtg gaactgtacg ccatagaagc gtttttcttc gttagccata
    16321 atggcaaacg ggcagctttc ggtgctggct acggtgacga agtcggacgg gatagcggta
    16381 actttatcgc cgtggctcat ccagacatcg agcagcggtt taccgtctgc ggtcagcgca
    16441 tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt caacctgcgc gtagccaaat
    16501 tcacgttcgt tagaggcttc aacgtgaccg cccaactgca ttgccatggt ctgcatgcca
    16561 tagcaaacgc cgaatatcgg tacgcctgct tcaaagacat actgcggcgc acgcggactg
    16621 ttttcttcag tagtactttc cgggccgccg gaaagaataa tgccgcttgg attgaagtca
    16681 cgaatttgtg cttctgtcac atcccacgcc cacagttcgc agtaaacacc cagctcacgc
    16741 acgcggcgcg caaccagttg agtgtactga gaaccgaagt ccagaatgag gatgcgatgc
    16801 ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa gtgaaacaga taatataaat
    16861 cgcccgacat aaagtcgggc gaagagaatc aggagcccag acggtagttc ggggactctt
    16921 tagtaatggt cacgtcgtga acgtggcttt cctgaatacc cgcaccgctg atacgtacaa
    16981 actccgcttt agtacgcagt tcgtcgatag taccacagcc ggtcagaccc atacaggagc
    17041 gcaggccgcc catctgctgg tgaatgatct ctttcaggcg acctttataa gctacgcgac
    17101 cttcgatacc ttccggcacc agtttgtcgg cagcgttatc gctctggaaa taacggtcag
    17161 aggaaccttt ggacatcgcg cccagggaac ccataccacg gtaagattta taagaacggc
    17221 cctggtagag ttcgatttca cccggagatt cttcagtacc cgccagcatg gaacctacca
    17281 tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc gccggagaag cgaataccac
    17341 catctgcgat aaccggaata ccggtgcctt ccagggcttc aactgcgtca gcaacggcag
    17401 tgatctgcgg aacacctacg ccagtaacga tacgagtcgt acagatagaa ccagggccga
    17461 taccgacttt aaccgcgctg caaccagctt ctgccaaagc gcgtgcacct gcagctgttg
    17521 ccacgttgcc gccgatgatt tgcagatccg gatatttagc acgggtttca cggatacgct
    17581 gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag cagaacgtca acgccagcgg
    17641 caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc gccaactgca gcaccaacac
    17701 gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg ttccgctttc tggaagtctt
    17761 tcacggtgat catgccgatc aggtggaatt cgtcatcaac caccagcgct ttttcaacgc
    17821 gtttttcgtg catttttgcc agcaccactt cacgggcttc accttcacgc acggtgacca
    17881 gacgctcttt cggcgtcatg taaacgctaa ccggctggtt caggtcggta acaaaacgca
    17941 cgtcacgacc ggtgataata cccaccagtt cgttttcttc ggtaacgacc ggatagcccg
    18001 caaaaccgtt acgctcggtc agttctttca cttcgcgcag cgtcgtggtt ggcagaacag
    18061 tctgcggatc agtcaccaca ccagattcgt gttttttcac acggcgaact tcttctgcct
    18121 ggcgttcaat ggacatgttt ttgtggataa agccgatacc gccttcctga gccagagcaa
    18181 tagccaggcg cgcttccgtt acggtatcca ttgctgcgga aagcataggg atattcagac
    18241 gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt cggcagaacg gtagaatgag
    18301 caggaacgag gagaacgtcg tcaaacgtca gagcttcttt agcgatacgt agcatgggca
    18361 atatctcgac cagagtggtt aataaatatt gccgcggcat tatacagagc gtaaccgatt
    18421 gcatctaccc ctttttgcaa aaaatgcttg ctatccccga agggcgggtt actatcgact
    18481 gaataacctg ctgatttaga atttgatctc gctcacatgt taccttctca atcccctgca
    18541 atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc tgcttgagca tgagatggga
    18601 caggtttgga tcagcggcga aatctctaat ttcacacaac cggcttccgg tcactggtac
    18661 tttacactca aagacgacac cgcccaggta cgctgcgcga tgttccgcaa cagcaaccgc
    18721 cgggtcacct tccgtccgca gcatgggcaa caagttttag ttcgcgccaa tattacgctc
    18781 tacgagccgc gcggcgacta ccagataatc gttgagagta tgcagccggc cggtgaaggg
    18841 ctgctgcaac agaagtacga acagctcaaa gcgaagttgc aggctgaagg tttgttcgat
    18901 cagcaataca aaaaaccact tccctcccct gcgcattgcg ttggtgtgat cacctcaaaa
    18961 accggtgctg cgctacatga tattttgcat gtgttaaaac gtcgcgatcc ttctctaccg
    19021 gtgatcatct accccacctc cgttcagggc gatgacgcgc cggggcaaat tgttcgcgcc
    19081 attgaactgg cgaatcagcg caatgagtgc gacgtgttga tcgttgggcg cggcggcggt
    19141 tcgctggaag atttatggag ttttaacgac gaacgcgtag cgcgggcgat ttttgccagc
    19201 cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg tgaccattgc cgattttgtt
    19261 gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag tagtgagccg taatcagcaa
    19321 gagttactgc gccaggtgca atcggcccgt caacggctgg agatggcgat ggattattat
    19381 ctcgccaacc gtacgcgtcg ctttacgcag attcatcatc gcttgcagca gcagcatccg
    19441 cagctccggc tggcacgcca gcaaaccatg cttgaacgcc tgcaaaaacg gatgagcttt
    19501 gcgctggaaa atcagcttaa gcgtgccggg caacagcagc agcgattaac acagcggctg
    19561 aatcagcaaa atccacagcc gaagattcat cgcacgcaaa cgcgcattca gcaactggaa
    19621 tatcgtttag cagaaatcct gcgcgcacag cttagcgcca cgcgtgaacg tttcggtaat
    19681 gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc tggcgcgtgg atacagcgtt
    19741 actactgcta ctgacggcaa tgtactgaaa aaagtgaagc aagttaaagc gggtgaaatg
    19801 ctaaccacac gtctggaaga cggctggata gaaagtgaag taaaaaacat ccagccggta
    19861 aaaaaatcgc gtaaaaaggt tcattaagcc agcacgaatt caacgcgttt tttcgatatc
    19921 aagccatgcc cgtgctgaca gaagtaatct actgcaccgc aggcttttaa cacctgcaac
    19981 ggttgatggc agtctggaca aagagctttc atttcaataa attccccgca ggatgggcaa
    20041 cgcgcatggc cgttatcctg atcaagcaca tgctgacatt ttggacaatg cagttccata
    20101 tgaattcctc cggtagccat tccctctaca ttcatagagg gaatggcaga taaaatactt
    20161 acggataatt atttattttt cttgatgtgc ttcatcagac gcttacgttt acgcatctgg
    20221 gttggcgtca gggtgttacg cttgttcgca tacgggtttt ccccttcttt gaactgaata
    20281 cgaatcggcg atcccattac gtccagcgat ttgcggaagt agttcatcaa gtagcgcttg
    20341 taggaatcag gcaggtcttt cacctgatta ccgtgaatca ccacaatcgg cgggttataa
    20401 ccaccagcgt gggcatattt cagcttcaca cgacgaccgc gtaccagcgg cggttggtga
    20461 tcttcaacag ccatcgtcat gatgcgcgtc agcatagagg tccccacacg acgggtggag
    20521 ctgtcatacg cttcacgtac tgattcaaac aagttaccaa caccactgcc gtgcaaggca
    20581 gagataaagt gcacacgagc aaaatcgata aagcccagac ggaagtccag cgtttctttc
    20641 acctgctctt tcacttcctg gctcaggcca tcccacttat tcaccacaat gacaagtgag
    20701 cgcccactat tgagaataaa gcccagcagc gagagatcct gatcggaaat accttcgcgc
    20761 gcatcaatca ctaacatcac cacgttggcg tcttcaatgg cctgcaacgt tttgattacg
    20821 gagaatttct ctacagcatc ggtgattttg ccgcgtttac gtacgccagc ggtgtcaatg
    20881 agcacatact cacgtccatc gcgttccatt gggatgtaga tgctgtcacg cgtcgtgcca
    20941 ggcatgtcgt aaacaacaac gcgctcttct ccaagaatac ggttagtgag tgtagactta
    21001 cctacgttcg gacgacccac aatcgccagt ttgatcggca gactttgcgg gtcgaagtcg
    21061 tcttcctctt cttcttcgcc gttctcttcc gcttcaaatt gcgcccagta ttcagcgtct
    21121 tcgtcgactt cctcttgcgg tgcgagatct tccatccacg gcagcagcac atgctccagc
    21181 agacttaata cgccacgacc gtgagacgcg gcgatcgggt agatttcacc taaaccaagc
    21241 gagtagaaat caaccactgc ctgatcggga tccagaccgt cagttttgtt tgccaccaag
    21301 aaggtcggtt tttcacggga gcgcagatgt ttggcaatcg cttcatctgc cggcatcagg
    21361 cccgcgcgcg catccaccat aaacagtacg acgtccgctt cttcaatcgc cagcagcgac
    21421 tgttccgcca tgcgggtttc tacaccgtct tctgtgccat caatcccgcc ggtatcaata
    21481 cagataaact cacggccttc aatttccgca cgaccgtact tacggtcacg agtcagaccc
    21541 gggaaatccg caaccagcgc atctcgggtg cgagttagac ggttaaataa cgtggatttt
    21601 cctacgttag ggcgcccgac aagcgcgacc acaggtacca tgtttaaagc ctcattttta
    21661 taaatcatca gacaacgcac gctatattcg cgtcgttgtt aaaaacagga aaacggcccc
    21721 tgtccaggag ccgttttcaa agtgaacgac agagacgatt aacgtgtaat agagtacacg
    21781 gttccgtctt ttgcctggat cagcagtttg ccgtcagcgg caaccggttc agtctggaaa
    21841 ccggaactat caactttttg ctgggcaacg aaacgaccat cttcgacgtt aatccagtgc
    21901 agataacctt cactgtcacc gaccaccagg ttgccattat acagcaccgg agaagtcagc
    21961 aggcgatgca gcagatcgct ttgtgcccac agcgtaacgc cgccatcaat ggtcaacgcc
    22021 atcacccggt cattttgatc gaccagatag atgcgattgc catcgacgat gaaatcattc
    22081 accgaaccca gttcgcgttt ccacataatc tgaccactgc gcagatcaag cgccgtcagg
    22141 ttaccattat aggccagcgc gaaaacaacg ccgttaacaa cgacgggagt cgtgtcaaca
    22201 tcgctcagac ggtcaatttc ggtagaaccg gtcgcctggg aaatacgctg ctgccaaatc
    22261 atctggccct gttccatcag cactgcgctg acgcgaccat tatcgccacc cacgacggcc
    22321 gcaccaaaag ccgttgccgg cgcagactcg ccacgcaaag agagcgaagg catatcgagg
    22381 ttaactgtcc atttgacagc gccgtcagct tcgttcagcg cttgtaactg accgttactg
    22441 gtgtggatta acaccagacc gtcgctgacc accgggcgcg aaagtgcttc acccgcgact
    22501 ttagtttgcc atgccacagt accatcgctg gtattcagcg cgtaaacctg cgccttttcg
    22561 ctgccaatgt agacatgccc accagacacg gtcacaccgc cagaaagtaa tgcaggctct
    22621 ttagagaacc agccatcttt ctcggccagg ctgacagacc agatttcttt gccatcatcc
    22681 gcattcagcg cttttaccaa accagcgcgg tccgctgcat agacaacgtt gtccgccagt
    22741 gccggatgaa gattggaata gaagttgcca atgccgctac caacggaagt gctccacgcc
    22801 gtggtcggcg taaactggtt ttcaacggtt ggcaatgggg acatctttac cacatcttct
    22861 tcgctgttaa acagcgaaca gccgcttaaa agggtaacgg aaagcagtcc tggcagcagt
    22921 aatttacgca attgcatcgg gtccctctca gatggacaaa ttattaattt tcatctgcat
    22981 catttcgctc agtgccggag taacatcgct tttcacgcct gcttcccatg cactacgcgc
    23041 accttgctta tcacctttgc tcagcaatgc ttcaccacgc aggtcggcaa caatggcagc
    23101 ccacccttca cctttgatgg tatcaagggt tttcagcgcg gcatcagcct gcttgagctg
    23161 tacctgaacg cgagcaagac gcagatttat cacggctttg agattttcat cgctcgtgtc
    23221 tgccagcccc tgttgtaact gggcggcagc tttctctagt tcatttttgt caacaaattg
    23281 ctgcgcaagt tccaaagaag ccagcgcacc ataagtattt ttattttcag cagcaaattt
    23341 ttccgccgcc gggatgctat ccggtttgcc ttcgctcact gcggtaaccg cattttgata
    23401 ggcaagagaa gcggagcgtg cagaatcaac ctgatggctg ttccagtagc gccagccaat
    23461 cagtgcgcca acgcccaaaa tcaccccaac agccagtgct ttgccatttt cagcaaaaaa
    23521 gcgtttaacc gcttctacct ggtcgttttc gttctcgtaa atttccacgc tgtccttctc
    23581 cttccttaac ccagtaacgt gcgcaaatgc gcggctacgc tatcctgcgc aactgccgtt
    23641 tgctcaccag agcgcaaatc cttcactact gctgtgccgt tagccacttc agactcaccc
    23701 agcaccacag caacgcgggc accccattta tcagcacggg caaactgttt cttaaagttg
    23761 ccgccgccgt ggttggtcat caatttcacg cccagtaatt catcacgcag acgctcagct
    23821 aatgccatag ccgcagattg tgtatcagca cctgaagcca ccaggtatat atcgacaaca
    23881 ggatcggctt taaattccgg attaacggcc tgtactaaca atacaagacg ttcgaggccc
    23941 atcgcaaaac cgacagccgg tgttgcacga ccgcccagtt gttccacaag accgtcataa
    24001 cgaccgcctg cacacacggt gccctgggag ccgagactgt tagtcaccca ctcgaaaacg
    24061 gtacggttat agtaatccag accacgcacc agacgctggt ttacggtgta agcgatcccc
    24121 gcgctctcca gctgtttgca cagaccggca aaatgctcac gagattcctc gtccagataa
    24181 tcacctaatg ccggagcgtc gttgagaagc gcctgcactt ccggattttt tgaatccagc
    24241 acgcgcagcg ggttagtgta catgcggcgt ttgcagtctt cgtccagctt ttctttatgc
    24301 tgctcaagga atgccaccag cgcatcgcgg taattggcgc gtgcttccag cgaaccaata
    24361 gaattcagct caagagttac gtgttcgaag atacccagcg cacgccacca gcgggcagtg
    24421 agcataatca gttcagcgtc gatatccgga ccttgcagac cgaaaacttc gcagcccaac
    24481 tgatggaact gacgataacg ccctttctgc ggacgctcgt gacggaacat cggcccgata
    24541 taccacagac gctgttcctg attgtacaga agaccatgct cgatgccggc gcgtacacag
    24601 cccgccgtcc cttcagggcg cagagtcagg ctgtcgccat tgcgatcctc aaaggtgtac
    24661 atctcttttt caaccacgtc ggtgacttca ccaatcgcac gtttgaatag cggggtctgc
    24721 tctacaatcg gcaagcggat ttcactgtaa ccgtagctgc cgagcacgtt tttcagtgtg
    24781 ccttcaatgc gctgccagat ggccgtttcg ccaggcaggt aatcgttcat gccgcgaatg
    24841 gcttgaatgt tttttgccac gtttattctc tttctgaata taaaaatgaa ccctcaacgc
    24901 ttccctcaat gtttcgggag ccatgcgggt tcaatcatac acgggaagcg aggcgcttcc
    24961 catcacgtta ttatttttca acctgctgaa cgtcaattcg acgcgcttcg tccagctgac
    25021 tggctttcgc acgaatgcgt gcttccagct ggtcgatcat atcgttgttg tccagacggt
    25081 ctttgcgcac gccatcttca tagaggccgc ttttcttgtt gccgccggtg acgccgagtg
    25141 tagaaaccag cgcctcacct gggccattca ccacgcagcc gataatcgaa acgtccatcg
    25201 gagtgatgat atcttccagg cgttgctcca gcgcgttaac cgtaccgata acatcaaatt
    25261 cctgacgcga acaggtcggg caggcgatga agttaatccc tcgcgaacgg atacgcagcg
    25321 atttcaaaat atcgaaaccg actttgatct cttcgaccgg atcggccgcc agcgatacgc
    25381 gcagcgtgtc gccgatgcct tccgacagca gcagacctaa accaatggcg gattttactg
    25441 ccccgctgcg tgcaccaccg gcttcggtga tccccagatg cagcggctga tcgatctgtt
    25501 ttgccagcaa acgataagac tcaacagcga ggaagacgtc agacgctttc acgctgactt
    25561 tgaactgatc gaagttcagg cgatcgagat gatcaacatg acgcatggcg gattccagca
    25621 acgcctgcgg cgtcggttcg ccatactttt cttgcagatc tttttccagc gatccggcgt
    25681 taacgccaat acggatcgga atgtttttat cgcgcgcaca gtcaaccacc atgcgaatac
    25741 gctcttcatt accgatatta ccagggttaa tacgcagaca atcgacgccg tattccgcta
    25801 ctttcagcgc aatgcgatag tcgaagtgga tgtcagccac cagcggcacg ttaacccgct
    25861 gtttgatgag tttgaacgct tctgccgcgt ccatcgtcgg tacggagaca cggacgatat
    25921 cagcgccaac gcgttccagc gccttgattt gattgaccgt tgcttcgaca tctgtcgtac
    25981 gcgtattggt catggactgt acggcgatgg gagcaccatc gccaatcggc acattcccaa
    26041 cgtaaatacg tgttgatttt ctacgttgaa ttggagcctg gttatgcatg aaaaatctcc
    26101 cgcgttaccc gtctgttact gcgccggtga ttgttcggca ttgagggtca gacgcgcaac
    26161 ctggttagtt ctgataaaac gactcagatc gacaggtttc ccttgatact ggatctgtac
    26221 tgcggctggc gcaccaattt tcagtttata cggtgcctgg ccggttaaat tcaaattacc
    26281 gtctttacgc tgcatcccgc taaacaattt tttaccggta gcatcagtga cctccagcca
    26341 gcaatcggca gtaaagttca tcaccagcgc attcggatca gccgccggcg tggtcacgcc
    26401 agcctgatcg gttggcaacg gcgcagcacc atctggcgtt gttgctgccg ttggtaccgg
    26461 ggtcgcggcg gtatcaacat ttgcctgcga aggcgaaaca accgcattct gttgcggatc
    26521 aacagctggt gctggcgcag ttacggcagg tgtttgcgtg ttggttgcgg tagtatccac
    26581 agacgctggc ggcgtggacg ttgtagccgg gtctgtagtt gtcgacgtat ttaacggaac
    26641 actctgcccc tgctcgctat tactgctcag ttccgccgaa gattgatcgg ccatagtggt
    26701 gatctcttcc tgctgagctt tgtggtcttg ccaccaccag gcaccgctca ggccgataac
    26761 cacaaacaac accagccagg tgaaggtcat cagccagccg tcgcgttttt tgcggcgttt
    26821 accgagggaa aaactctgca ttggcgcaac ttttgcagcc cgaagtggag cctgcttttc
    26881 cagccctggc agcagttctt cttctggaat atgtaccaga cgcgcataag agcggatata
    26941 tccgcgcagg aatgttgaag caagatcggc gggtgcctta tcttcttcaa tgtcgcgtac
    27001 cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgc tgactaagtc ctagttgttc
    27061 gcgagcatta cgcaggcgag cgccggtagt aagtgcttca ttttggtcgt gcgtggcttc
    27121 agtattcatt cgctgcaggt acgtttaaat gagaattgag atgccggtga accatcatgc
    27181 tcacccacat cgcgaaacat ccgttaagtt aaccgttatc atacagtata agaccgtctg
    27241 tgtgctcgtg acaaagccca cattcactga ggctaactta ctgttgcatc gttacatact
    27301 gccttaaagt cagcaaaaac gcaccgttaa tattgaccag acaattgcaa cttaatactt
    27361 cattcataaa agattacgcc acggtacata aagtaaccgt ggcgtaatgg ctatcagacc
    27421 gctttaatat cgatggcttc accctgcatc cgtttacgca gagtacgttt cgtacggtcg
    27481 ataacatcgc ccgccaactg accacaggca gcatcgatat catcaccacg cgttttacgc
    27541 acaatagtgg tgaaaccgta gctcatcagc acttttgaga aacggtcgat acggctgttc
    27601 gaactgcgtc catacggcgc gcccgggaac gggttccacg ggatcaggtt gatcttacac
    27661 ggcgtatctt tcagcagttc cgccagttgg tgcgcgtgtt cagtgccgtc gttaacgtgg
    27721 tcaagcatca cgtattcaat agtgactcgg ccctgattgg cgttggattt ctccagataa
    27781 cggcgcaccg cagcaaggaa cgtttcgata ttgtactttt tgttgatcgg cacaatttcg
    27841 tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca gtgcaacgtc gatcatatcg
    27901 cccagtttat ccagcgccgg aactacaccg gaagtggaaa gcgtcacgcg acgtttagac
    27961 aggccaaaac cgaaatcatc aagcatgatt tccatcgccg gaacgacgtt gttcaggttg
    28021 agcagcggct cgcccatgcc catcatcacg acgttagtga ttgggcgctg accggtgact
    28081 tttgctgcgc cgacgatttt cgccgcacgc cacacctggc caataatttc cgatacccgc
    28141 aggttgcggt taaagccctg ctgggcggtg gaacagaatt tacactccag cgcacacccc
    28201 acctgcgaag agacgcagag cgtggcacgg tcgtcttccg ggatatacac cgtttcgacg
    28261 cgctgatcgc caacggcgat cgcccattta atggtgccgt cggatgaacg ctgttcttca
    28321 accacttccg gtgcgcggat ttccgccacc tctttcagtt tgccgcgcaa cactttgttg
    28381 atgtcggtca tctcatcaaa gttgtcgcag caatagtgat acatccactt catcacctga
    28441 tcggcgcgga aggttttttc acctaaatct ttaaaaaact cccgcatctg ctgacggttg
    28501 agatccagca ggttgatttt tccatctttc gtggtgacgt tttcaggtgt gactaattgt
    28561 tcagacatat gctattccgg cctcgttatt acacgttatg gcccctggag ggttgaaaaa
    28621 agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg cattgtacaa attctggcgc
    28681 acggatgcca cgtttgcacg cggcatttac gaaattatta acgggtgcgc gggcacactt
    28741 cgccttcgcc gaagaaataa gcgatttcgc gagcggcaga ttcgacggaa tcagaaccgt
    28801 gggtaccgtt ttcggtcagg ctgtcagcgt aatcagcgcg cagagtacca gccagtgcgt
    28861 ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg aacggcgttt tcaccttcca
    28921 gcacggaaac cacgatcggg ccagaggtca tgaattcaac cagaccatca aagaacggtt
    28981 ttccatcgtg ttcagcataa aagccacgtg cctgttcaac ggtcaggtgc agcattttgg
    29041 tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa gatattacca atgacgtttt
    29101 ttgctaccgc gttcggtttg atgatggaaa aagtacgttc aatagccatt tttacctctg
    29161 taaattgttc tgttgttgtc tgtaccagcg tacgagatgg cgcggattat aatgagcaac
    29221 agggccgttg actattgatg aaggtaacat ttttttaaaa taaaatgagt tttagcaaca
    29281 atcagggaca aaacagacta ttgcatgaca aatttcactg tcgcgatttg ccccacatca
    29341 tccatcacca gcaattgata atcaccttta tccgtcaaat gcagggtcac gttgcgcccg
    29401 cgttcagtta acggttcgcc attcagaaac caccagcgtt cacctgcccc tccacttgat
    29461 tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt taataatcgc gccatcgcga
    29521 acgcctgtca gctgcagagg gagctgggca tcgtgaccgt atggcgggca aattgtcgag
    29581 gccggtggta agcgcacagc gcgacgttct gatgcgggca gccagggttc cagcggtagc
    29641 ggccagacat ttaccatttc ttgcttcgcc tgcgggcagt cagcggcgac gcgctgccct
    29701 ttgtcatcca gccagatggg gaagcgaatg ccattaatcc cttcctgctc tgacagtaac
    29761 agcgttggcg gctggctgcc atccagcagc caggttgcca ggcggcggcg acagttaccg
    29821 tcaccttccg gcaaagactg tccgcccggc cagcagataa cgccacgagt gactgagttc
    29881 gggcgcgggt cttccggcag attcgcactg cgcgacagta agatattatt gacctgattc
    29941 aacaatggta cggcactggc aaagccaaac tgaccaacaa cgggcgtgcc gtcaggtctg
    30001 ccagtccaga tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc gtcacgatag
    30061 ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc actatccggc
    30121 aagggttgcg cttcatcagc cataatccgg cgaatgatcc acgccgcccc cgacgacatt
    30181 aaaggccgtt caagcagcgg atcgtcaggc tgtaagcgca atttgcctgc cttgccgtgg
    30241 cgagcaaacg cggtatacgc tgccgccata tcttccagtt ttgcaccagc gccgccgaga
    30301 atgagtgaaa gattcggcgc agcgccgttg ggcaaatata acagcaatcc aacattgcgt
    30361 aactttgccg caaaccgttt cggtccatag gcttccagca cctgcacagc aggtaagttc
    30421 agcgagcgca ccagcgcctc gctcatgttg atcgggccat gaaaaccgct atcaaagtta
    30481 cctagtcgat aatcaccggt gcgccggggg acgtcttgca gcagtgatgc cgggtggatc
    30541 aagccttcat ccagcgccag accataaaca aacggtttga gcaccgatcc tggcgatcgg
    30601 atcgcattga ccatatcaac atgaccaaag cgtgaatcat cgttgagatc aaccgatccc
    30661 acccagccgc gaacacgcat atcggtatga ttaaccacga tcatcgccag tgagctgcgc
    30721 ggtggcaacc gccctttcca gttttgcgcc agttcttcca gacgtcgttg aagaccggca
    30781 tccagcgtag taacgatttt gtcgcttttg cttttaccga gcatcatgcg cgaaaacagc
    30841 ggtgccagtt gcggcatttg tcggggtgcc agccagatgg gttcttccct tgactctttt
    30901 acctgctcac gggaccacac accttgcgca gccatccgtt cgagcacttt attacgcgta
    30961 ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gcagtcggct gggcgcttgc
    31021 ggtaacaccg ccagcattgc cgcctaggaa tagcttaaat tcgcaggcga ttttccgaga
    31081 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
    31141 tacaagatca gaatttcacg cttagacaaa tgccactcca gttgcaacgc gcgccagagc
    31201 tggagaattt tgccaccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
    31261 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
    31321 gccaccgaga atggattaac ccccggatgc ttccagaacc agcgatcttc ataattgatc
    31381 agcgcttcaa ggtaacgtgc ccctattaag ttgtcaagca tgttatgacc cctgcggggt
    31441 ataaaaagtc ccgtcgcgca tcatggcgaa cagaacgtcg cagcgtcgtc tcgccagggc
    31501 gataagcacc tgattgtgtc gttttccctg actcattttg cgggtgtagt aag
//
LOCUS       DADLSE010000036        30108 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLSE010000036 DADLSE010000000
VERSION     DADLSE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.036"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            269..652
                     /gene="secE"
                     /locus_tag="JN692_001621"
     CDS             269..652
                     /gene="secE"
                     /locus_tag="JN692_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7263959.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            654..1199
                     /gene="nusG"
                     /locus_tag="JN692_001622"
     CDS             654..1199
                     /gene="nusG"
                     /locus_tag="JN692_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7263960.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1358..1786
                     /gene="rplK"
                     /locus_tag="JN692_001623"
     CDS             1358..1786
                     /gene="rplK"
                     /locus_tag="JN692_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7263961.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1790..2494
                     /gene="rplA"
                     /locus_tag="JN692_001624"
     CDS             1790..2494
                     /gene="rplA"
                     /locus_tag="JN692_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7263962.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2907..3404
                     /gene="rplJ"
                     /locus_tag="JN692_001625"
     CDS             2907..3404
                     /gene="rplJ"
                     /locus_tag="JN692_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7263963.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3471..3836
                     /gene="rplL"
                     /locus_tag="JN692_001626"
     CDS             3471..3836
                     /gene="rplL"
                     /locus_tag="JN692_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7263964.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4156..8184
                     /gene="rpoB"
                     /locus_tag="JN692_001627"
     CDS             4156..8184
                     /gene="rpoB"
                     /locus_tag="JN692_001627"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7263965.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8261..12484
                     /gene="rpoC"
                     /locus_tag="JN692_001628"
     CDS             8261..12484
                     /gene="rpoC"
                     /locus_tag="JN692_001628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7263966.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12726..13859)
                     /gene="thiH"
                     /locus_tag="JN692_001629"
     CDS             complement(12726..13859)
                     /gene="thiH"
                     /locus_tag="JN692_001629"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7263967.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13856..14701)
                     /gene="thiG"
                     /locus_tag="JN692_001630"
     CDS             complement(13856..14701)
                     /gene="thiG"
                     /locus_tag="JN692_001630"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7263968.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIADKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14628..14828)
                     /gene="thiS"
                     /locus_tag="JN692_001631"
     CDS             complement(14628..14828)
                     /gene="thiS"
                     /locus_tag="JN692_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7263969.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14812..15567)
                     /gene="thiF"
                     /locus_tag="JN692_001632"
     CDS             complement(14812..15567)
                     /gene="thiF"
                     /locus_tag="JN692_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7263970.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15560..16195)
                     /gene="thiE"
                     /locus_tag="JN692_001633"
     CDS             complement(15560..16195)
                     /gene="thiE"
                     /locus_tag="JN692_001633"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7263971.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16195..18090)
                     /gene="thiC"
                     /locus_tag="JN692_001634"
     CDS             complement(16195..18090)
                     /gene="thiC"
                     /locus_tag="JN692_001634"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7263972.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18171..18271)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18324..18800)
                     /gene="rsd"
                     /locus_tag="JN692_001635"
     CDS             complement(18324..18800)
                     /gene="rsd"
                     /locus_tag="JN692_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7263973.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18895..19668
                     /gene="nudC"
                     /locus_tag="JN692_001636"
     CDS             18895..19668
                     /gene="nudC"
                     /locus_tag="JN692_001636"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7263974.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19708..20772
                     /gene="hemE"
                     /locus_tag="JN692_001637"
     CDS             19708..20772
                     /gene="hemE"
                     /locus_tag="JN692_001637"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7263975.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20929..21600
                     /gene="nfi"
                     /locus_tag="JN692_001638"
     CDS             20929..21600
                     /gene="nfi"
                     /locus_tag="JN692_001638"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7263976.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21643..22233
                     /locus_tag="JN692_001639"
     CDS             21643..22233
                     /locus_tag="JN692_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7263977.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22420..22692
                     /gene="hupA"
                     /locus_tag="JN692_001640"
     CDS             22420..22692
                     /gene="hupA"
                     /locus_tag="JN692_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7263978.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22705..23400
                     /locus_tag="JN692_001641"
     CDS             22705..23400
                     /locus_tag="JN692_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7263979.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23402..23827)
                     /gene="zraP"
                     /locus_tag="JN692_001642"
     CDS             complement(23402..23827)
                     /gene="zraP"
                     /locus_tag="JN692_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7263980.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24065..25441
                     /gene="zraS"
                     /locus_tag="JN692_001643"
     CDS             24065..25441
                     /gene="zraS"
                     /locus_tag="JN692_001643"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7263981.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25438..26763
                     /gene="zraR"
                     /locus_tag="JN692_001644"
     CDS             25438..26763
                     /gene="zraR"
                     /locus_tag="JN692_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7263982.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCATLNESLLESELFGHEKGAF
                     AGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQVMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26760..28049)
                     /gene="purD"
                     /locus_tag="JN692_001645"
     CDS             complement(26760..28049)
                     /gene="purD"
                     /locus_tag="JN692_001645"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7263983.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28061..29650)
                     /gene="purH"
                     /locus_tag="JN692_001646"
     CDS             complement(28061..29650)
                     /gene="purH"
                     /locus_tag="JN692_001646"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7263984.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7053 a         7850 c         8281 g         6924 t
ORIGIN      
        1 accgttggcg cgggcgttgt tgctaaagtt ctgagctaat tgccgataac atttgacgca
       61 atgcgcacta aaagggcatc atttgatgcc ctttttgcac gctttcgtac cagaacctgg
      121 ctcatcagtg attttctttg tcataatcat tgctgagaca ggctctgttg agggcgtata
      181 atccgaaaag ctaatacgcg tttcgatttg gtttgcctcg cgatcgcggg gtgaaaatgt
      241 ttgtagaaaa cttctgacag gttggtttat gagtgcgaat accgaagctc aaggaagcgg
      301 gcgcggcctg gaagcgatga agtgggtcgt tgtggtggca ttgctcctgg tggcgattgt
      361 cggcaactac ctttatcgcg acattatgct gccgctgcgt gcgctggccg tagtaattct
      421 gattgctgca gcgggtggtg tcgcgctgtt aacgacaaaa ggtaaagcta ccgttgcttt
      481 tgcccgtgaa gcgcgtaccg aagtccgtaa ggtcatttgg ccgactcgcc aggaaacatt
      541 gcacaccacg ctgattgtgg ctgcggttac cgcagtaatg tcactgatcc tgtggggact
      601 ggatggtatt ctggttcgcc tggtatcctt tatcactggc ctgaggttct gagatgtctg
      661 aagctcctaa aaagcgctgg tacgtcgttc aggcgttttc cggttttgaa ggccgcgtag
      721 caacgtcgct gcgtgagcat atcaaattac acaacatgga agatttgttt ggtgaagtca
      781 tggtaccaac cgaagaagtg gttgaaatcc gtggcggtca gcgtcgcaaa agcgaacgta
      841 aattcttccc tggctacgtc ctcgttcaga tggtgatgaa cgacgcgagc tggcacttgg
      901 tgcgcagcgt accgcgtgtg atgggcttca tcggcggtac ttccgatcgt cctgcgccaa
      961 tcagcgataa agaagtcgat gcgattatga accgcctgca gcaggttggt gataagccgc
     1021 gtccgaaaac gctgtttgaa ccgggtgaaa tggtccgtgt taatgatggt ccgttcgctg
     1081 acttcaacgg tgttgttgaa gaagtggatt acgagaaatc tcgtctgaaa gtgtctgttt
     1141 ctatcttcgg tcgtgcgacc ccggtagagc tggacttcag ccaggttgaa aaagcctaac
     1201 ccagcgatca aaaaagcggc gatttaatcg ttgcacaagg cgtgagattg gaatacaatt
     1261 tcgcgccttt tgtttttatg ggccttgccc gtaaaacgat tttttatatc acggggagcc
     1321 tctcagaggc gttattaccc aacttgagga atttataatg gctaagaaag tacaagccta
     1381 tgtcaagctg caggttgcag ctggtatggc taacccgagt ccgccagtag gtccggctct
     1441 gggtcagcag ggcgtaaaca tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc
     1501 catcgaaaaa ggtctgccga ttccggtagt aatcaccgtt tacgctgacc gttctttcac
     1561 tttcgttacc aagaccccgc cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc
     1621 tggttccggt aagccgaaca aagacaaagt gggtaaaatt tcccgcgctc agctgcagga
     1681 aatcgcgcag accaaagctg ccgacatgac tggtgccgac attgaagcaa tgactcgctc
     1741 catcgaaggt actgcacgtt ccatgggcct ggtagtggag gactaagaaa tggctaaact
     1801 gaccaagcgc atgcgtgtta tccgcgagaa agttgatgca accaaacagt acgacatcaa
     1861 cgaagctatc gcactgctga aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt
     1921 agctgttaac ctcggcatcg acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt
     1981 actgccgcac ggtactggcc gttccgttcg cgtagccgta tttacccaag gtgcaaacgc
     2041 tgaagctgct aaagctgcag gcgcagaact ggtaggtatg gaagatctgg ctgaccagat
     2101 caagaaaggc gaaatgaact ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt
     2161 tggccagctg ggccaggttc tgggtccgcg cggcctgatg ccaaacccga aagtgggtac
     2221 tgtaacaccg aacgttgctg aagcggttaa aaacgctaaa gctggccagg ttcgttaccg
     2281 taacgacaaa aacggcatca tccacaccac catcggtaaa gtggactttg acgctgacaa
     2341 actgaaagaa aacctggaag ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc
     2401 gaaaggcgtg tacatcaaga aagttagcat ctccaccacc atgggtgcag gtgttgcagt
     2461 tgaccaggct ggcctgagcg cttctgtaaa ctaatgcctt tacgtgggag gtgattttgt
     2521 ctacaatctt acccccacgt ataatgctta atgcagacgt atatccgaga tattcgggtt
     2581 gtggcaaggc ggcaactgag tgagtcgcca gtcacatagc taactatgtg actggtgcga
     2641 atgaaggaag ccaacgccgc cacaagttga atagcgacgg atagaaaaga tttgttcgtt
     2701 ggagcctggc ctatccaggc ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc
     2761 ccctgcgtag acggtgacag aacgctaaga ttattctttt atattctggc ttgtttctgc
     2821 tcaccgtaat taagacgctc tctccgtttg gaggagtgaa gtgagttcca gagattttct
     2881 ctggcaaaca tccaggagca aagctaatgg ctttaaatct tcaagacaaa caagcgattg
     2941 ttgctgaagt cagcgaagta gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg
     3001 gcgtaactgt agataaaatg actgaactgc gtaaagcagg tcgcgaagct ggcgtataca
     3061 tgcgtgttgt tcgtaacacc ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc
     3121 tgaaagacgc gtttgttggt ccgaccctga ttgcatactc tatggaacac ccgggcgctg
     3181 ctgctcgtct gttcaaagag ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg
     3241 cctttgaagg tgagctgatc ccggcgtctc agatcgaccg cctggcaact ctgccgacct
     3301 acgaagaagc aattgcacgc ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg
     3361 ttcgtactct ggctgctgta cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt
     3421 aacgcattcg cttacgtata aacttattct gatattcagg aacaatttaa atgtctatca
     3481 ctaaagatca aatcattgaa gcagttgcag ctatgtctgt aatggacgtt gtagaactga
     3541 tctctgcaat ggaagaaaaa ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg
     3601 gcccggttga agctgctgaa gaaaaaactg aattcgacgt aattctgaaa gctgctggcg
     3661 ctaacaaagt tgctgttatc aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag
     3721 ctaaagacct ggtagaatct gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg
     3781 cagaagcact gaaaaaagct ctggaagaag ctggcgctga agttgaagtt aaataagcca
     3841 acccttccgg ttgcagcctg agaaatcagg ctgatggctg gtgacttttt agtcaccagc
     3901 ctttttgcgc tgtaaggcgc cagtagcgtt tcacactgtt tgactactgc tgtgcctttc
     3961 aatgcttgtt tctatcgacg acttaatata ctgcgacagg acgtccgttc tgtgtaaatc
     4021 gcaatgaaat ggtttaagcg tgatagcaac aggcattgcg gaaagtgttc cattttccgg
     4081 tcaacaaaat agtgttgcac aaactgtccg ctcaatggac agatgggtcg acttgtcagc
     4141 gagctgagga accctatggt ttactcctat accgagaaaa aacgtattcg taaggatttt
     4201 ggtaaacgtc cacaagttct ggatgtacct tatctccttt ctatccagct tgactcgttt
     4261 cagaaattta tcgagcaaga tcctgaaggg cagtatggtc tggaagctgc tttccgttcc
     4321 gtattcccga ttcagagcta cagcggtaat tccgagctgc aatacgtcag ctaccgcctt
     4381 ggcgaaccgg tgtttgacgt ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg
     4441 ctgcgcgtta aactgcgtct ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa
     4501 gacattaaag aacaagaagt ctacatgggc gaaattccgc tcatgacaga caacggtacc
     4561 tttgttatca acggtactga gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc
     4621 ttctttgact ccgacaaagg taaaacccac tcttcgggta aagtgctgta taacgcgcgc
     4681 atcatccctt accgtggttc ctggctggac ttcgaattcg atccgaagga caacctgttc
     4741 gtacgtatcg accgtcgccg taaactgcct gcgaccatca ttctgcgcgc cctgaactac
     4801 accacagagc agatcctcga cctgttcttt gaaaaagtta tctttgaaat ccgtgataac
     4861 aagctgcaga tggaactggt gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc
     4921 gaagctaacg gtaaagtgta cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc
     4981 cagctggaaa aagacgacgt caaactgatc gaagtcccgg ttgagtacat cgcaggtaaa
     5041 gtggttgcta aagactatat tgatgagtct accggcgagc tgatctgcgc agcgaacatg
     5101 gagctgagcc tggatctgct ggctaagctg agccagtctg gtcacaagcg tatcgaaacg
     5161 ctgttcacca acgatctgga tcacggccca tatatctctg aaaccttacg tgtcgaccca
     5221 actaacgacc gtctgagcgc actggtagaa atctaccgca tgatgcgccc tggcgagccg
     5281 ccgactcgtg aagcagctga aagcctgttc gagaacctgt tcttctccga agaccgttat
     5341 gacttgtctg cggttggtcg tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa
     5401 ggttccggta ttctgagcaa agacgacatc attgatgtta tgaaaaagct catcgatatc
     5461 cgtaacggta aaggcgaagt cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc
     5521 gttggcgaaa tggcggaaaa ccagttccgc gttggcctgg tacgtgtaga gcgtgcggtg
     5581 aaagagcgtc tgtctctggg cgatctggat accctgatgc ctcaggatat gatcaacgcc
     5641 aagccgattt ccgcagcagt gaaagagttc ttcggttcca gccagctgtc tcagtttatg
     5701 gaccagaaca acccgctgtc tgagattacg cacaaacgtc gtatctccgc actcggccca
     5761 ggcggtctga cccgtgaacg tgcaggcttc gaagttcgag acgtacaccc gactcactac
     5821 ggtcgcgtat gtccaatcga aacccctgaa ggtccgaaca tcggtctgat caactctctg
     5881 tccgtgtacg cacagactaa cgaatacggc ttccttgaga ctccgtatcg taaagtgacc
     5941 gacggtgttg taactgacga aattcactac ctgtctgcta tcgaagaagg caactacgtt
     6001 atcgcccagg cgaactccaa cctggatgaa gaaggccact tcgtagaaga cctggtaact
     6061 tgccgtagca aaggcgaatc cagcttgttc agccgcgacc aggttgacta catggacgta
     6121 tccacccagc aggtggtatc cgtcggtgcg tccctgatcc cgttcctgga acacgatgac
     6181 gccaaccgtg cattgatggg tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct
     6241 gataagccgc tggttggtac tggtatggaa cgtgctgttg ccgttgactc cggtgtaact
     6301 gcggtagcta aacgtggtgg tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa
     6361 gttaacgaag acgagatgta tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa
     6421 tacacccgtt ctaaccagaa cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa
     6481 ccggttgaac gtggcgacgt gctggcagac ggtccgtcca ccgacctcgg tgaactggcg
     6541 cttggtcaga acatgcgcgt agcgttcatg ccgtggaatg gttacaactt cgaagactcc
     6601 atcctcgtat ccgagcgtgt tgttcaggaa gaccgtttca ccaccatcca cattcaggaa
     6661 ctggcgtgtg tgtcccgtga caccaagctg ggtccggaag agatcaccgc tgacatcccg
     6721 aacgtgggtg aagctgcgct ctccaaactg gatgaatccg gtatcgttta cattggtgcg
     6781 gaagtgaccg gtggcgacat tctggttggt aaggtaacgc cgaaaggtga aactcagctg
     6841 accccagaag aaaaactgct gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac
     6901 tcttctctgc gcgtaccaaa cggtgtatcc ggtacggtta tcgacgttca ggtctttact
     6961 cgcgatggcg tagaaaaaga caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag
     7021 gcgaagaaag acctgtctga agaactgcag atcctcgaag cgggtctgtt cagccgtatc
     7081 cgtgctgtgc tggtagccgg tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat
     7141 cgctggctgg agctgggcct gacagacgaa gagaaacaaa atcagctgga acagctggct
     7201 gagcagtatg acgaactgaa acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa
     7261 atcacccagg gcgacgatct ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg
     7321 gttaaacgcc gtatccagcc tggtgacaag atggcaggtc gtcacggtaa caagggtgta
     7381 atttctaaga tcaacccgat cgaagatatg ccttacgatg aaaacggtac gccggtagac
     7441 atcgtactga acccgctggg cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc
     7501 cacctgggta tggctgcgaa aggtatcggc gacaagatca acgccatgct gaaacagcag
     7561 caagaagtcg cgaaactgcg cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt
     7621 cgtcagaaag ttgacctgag taccttcagc gatgaagaag ttatgcgtct ggctgaaaac
     7681 ctgcgcaaag gtatgccaat cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt
     7741 aaagagctgc tgaaacttgg cgacctgccg acttccggtc agatccgcct gtacgatggt
     7801 cgcactggtg aacagttcga gcgtccggta accgttggtt acatgtacat gctgaaactg
     7861 aaccacctgg tcgacgacaa gatgcacgcg cgttccaccg gttcttacag cctggttact
     7921 cagcagccgc tgggtggtaa ggcacagttc ggtggtcagc gtttcgggga gatggaagtg
     7981 tgggcgctgg aagcatacgg cgcagcatac accctgcagg aaatgctcac tgttaagtct
     8041 gatgacgtga acggtcgtac caagatgtat aaaaacatcg tggacggcaa ccatcagatg
     8101 gagccgggca tgccagaatc cttcaacgta ttgttgaaag agattcgttc gctgggtatc
     8161 aacatcgaac tggaagacga gtaattctcg ctcaaacagg tcactgctgt cggggtaaaa
     8221 cccggcagcg gattgtgcta actccgacgg gagcaaatcc gtgaaagatt tattaaagtt
     8281 tctgaaagcg cagactaaaa ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc
     8341 agacatgatc cgttcatggt ctttcggtga agttaaaaag ccggaaacca tcaactaccg
     8401 tacgttcaaa ccagaacgtg acggcctttt ctgcgcccgt atctttgggc cggtaaaaga
     8461 ttacgagtgc ctgtgcggta agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa
     8521 gtgcggcgtt gaagtgaccc agactaaagt acgccgtgag cgtatgggcc acatcgaact
     8581 ggcttccccg actgcgcaca tctggttcct gaaatcgctg ccgtcccgta tcggtctgct
     8641 gctcgatatg ccgctgcgcg atatcgaacg cgtactgtac tttgaatcct atgtggttat
     8701 cgaaggcggt atgaccaacc tggaacgtca gcagatcctg actgaagagc agtatctgga
     8761 cgcgctggaa gagttcggtg acgaattcga cgcgaagatg ggtgcggaag caatccaggc
     8821 tctgctgaag agcatggatc tggagcaaga gtgcgaacag ctgcgtgaag agctgaacga
     8881 aaccaactcc gaaaccaagc gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt
     8941 cgttcagtct ggtaacaaac cagagtggat gatcctgacc gttctgccgg tactgccgcc
     9001 agatctgcgt ccgctggttc cgctggatgg tggtcgtttc gcgacttctg acctgaacga
     9061 tctgtatcgt cgcgtcatta accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc
     9121 gccggacatc atcgtacgta acgaaaaacg tatgctgcag gaagcggtag acgccctgct
     9181 ggataacggt cgtcgcggtc gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt
     9241 ggccgacatg atcaaaggta aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt
     9301 tgactactcc ggtcgttctg taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg
     9361 tctgccgaag aaaatggcgc tggagctgtt caaaccgttc atctacggca agctggaact
     9421 gcgtggtctt gctaccacca ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt
     9481 cgtttgggat atcctggacg aagttatccg cgaacacccg gtactgctga accgtgcacc
     9541 gactctgcac cgtctgggta tccaggcatt tgaaccggta ctgatcgaag gtaaagctat
     9601 ccagctgcac ccgctggttt gtgcggcata taacgccgac ttcgatggtg accagatggc
     9661 tgttcacgta ccgctgacgc tggaagccca gctggaagcg cgtgcgctga tgatgtctac
     9721 caacaacatc ctgtccccgg cgaacggcga accaatcatc gttccgtctc aggacgttgt
     9781 actgggtctg tactacatga cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct
     9841 gactggcccg aaagaagcag aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg
     9901 cgttaaagtg cgtatcaccg agtatgaaaa agatgctaac ggtgaattag tagcgaaaac
     9961 cagcctgaaa gacacgactg ttggccgtgc cattctgtgg atgattgtac cgaaaggtct
    10021 gccttactcc atcgtcaacc aggcgctggg taaaaaagca atctccaaaa tgctgaacac
    10081 ctgctaccgc attctcggtc tgaaaccgac cgttattttt gcggaccaga tcatgtacac
    10141 cggcttcgcc tatgcagcgc gttctggtgc atctgttggc atcgatgaca tggtcatccc
    10201 ggagaagaaa cacgaaatca tctccgaggc agaagcagaa gttgctgaaa ttcaggagca
    10261 gttccagtct ggtctggtaa ctgcgggcga acgctacaac aaagttatcg atatctgggc
    10321 tgcggcgaac gatcgtgtat ccaaagcgat gatggataac ctgcaaactg aaaccgttat
    10381 taaccgtgac ggtcaggaag agaagcaggt ttccttcaac agcatctaca tgatggccga
    10441 ctccggtgcg cgtggttctg cggcacagat tcgtcagctt gctggtatgc gtggtctgat
    10501 ggcgaagccg gatggctcca tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct
    10561 gaacgtactc cagtacttca tctccaccca cggtgctcgt aaaggtctgg cggataccgc
    10621 actgaaaact gcgaactccg gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct
    10681 agtggttacc gaagacgatt gtggtaccca tgaaggtatc atgatgactc cggttatcga
    10741 gggtggtgac gttaaagagc cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga
    10801 cgttctgaag ccgggtactg ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca
    10861 gtggtgtgac ctgctggaag agaactctgt cgacgcggta aaagtacgtt ctgttgtatc
    10921 ttgtgacacc gactttggtg tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca
    10981 catcatcaac aaaggtgaag caatcggtgt tatcgcggca cagtccatcg gtgaaccggg
    11041 tacacagctg accatgcgta cgttccacat cggtggtgcg gcatctcgtg cggctgctga
    11101 atccagcatc caggtgaaaa acaaaggtag catcaagctc agcaacgtga agtcggttgt
    11161 gaactccagc ggtaaactgg ttatcacttc ccgtaacacc gaactgaaac tgatcgacga
    11221 attcggtcgt accaaagaaa gctacaaagt gccttacggt gcggtactgg cgaaaggcga
    11281 tggcgaacag gttgctggcg gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt
    11341 tatcaccgaa gtaagcggtt ttgtacgctt tactgacatg atcgacggcc agaccattac
    11401 tcgtcagacc gacgaactga ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg
    11461 taccgcaggt ggtaaagatc tgcgtccggc actgaaaatc gttgatgctc agggtaacga
    11521 cgttctgatc ccaggtaccg atatgcctgc gcagtacttc ctgccgggta aagcgattgt
    11581 tcagctggaa gatggcgtac agatcagctc tggtgacacc ctggcgcgta ttccgcagga
    11641 atccggcggt accaaggaca tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc
    11701 tcgtcgtccg aaagagccgg caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa
    11761 agaaaccaaa ggtaaacgtc gtctggttat caccccggta gacggtagcg atccgtacga
    11821 agagatgatt ccgaaatggc gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg
    11881 tgacgtaatt tccgacggtc cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca
    11941 tgctgtgact cgttacatcg ttaacgaagt acaggacgta taccgtctgc agggcgttaa
    12001 gattaacgat aaacacatcg aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt
    12061 taacgcgggc agctccgact tcctggaagg cgaacaggtt gaatactctc gcgtcaagat
    12121 cgcaaaccgc gaactggaag cgaacggcaa agtgggtgca acttactccc gcgatctgct
    12181 gggtatcacc aaagcgtctc tggcaaccga gtccttcatc tccgcggcat cgttccagga
    12241 gaccactcgc gtgctgaccg aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct
    12301 gaaagagaac gttatcgtgg gtcgtctgat cccggcaggt accggttacg cgtaccacca
    12361 ggatcgtatg cgtcgccgtg ctgcgggtga agctccggct gcaccgcagg tgactgcaga
    12421 agacgcatct gccagcctgg cagaactgct gaacgcaggt ctgggcggtt ctgataacga
    12481 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggtgtt
    12541 attcagaggg caaattcttc acggaaaata ttaagaaagg cagggcgaaa cgaggaagaa
    12601 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    12661 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    12721 ccgtctcata gtctttgcga ggcgcgtccc agatagctgt cccagtcttt ccataccggc
    12781 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    12841 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    12901 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    12961 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13021 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13081 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    13141 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    13201 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    13261 tgttttttgc ctttcaaatg atggcgggta taagtcgcct cgtgatatgt ctcctgataa
    13321 accatcaccc catccagacc aagttgcttt aactcggcgt attccgtctc cgccagcggt
    13381 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    13441 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    13501 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    13561 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    13621 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    13681 tgttgggcca gttgttccag atagccactg gcggcaggcg ataacagcgc catcatgtcg
    13741 tcgcgggtga gttgcgaggc atttagcgcc cgctctacgt cagcagccgt tttgccgttg
    13801 atacgcaggc ggatgtcgtc ccagtccagt tgtcgccagc gattgctgaa ggttttcatg
    13861 ccgatgcctc cagaaatccg gtcagcgggc tggtggcatg agcaaaataa ctgcggctgc
    13921 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    13981 tgttgacggg atcgtccgcg acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14041 tttccagcgc ctgcgcggca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    14101 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    14161 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    14221 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcgtttcgg
    14281 cggctttcag ggtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcca
    14341 attttagcca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    14401 ccgctgtttt cgccccggaa gtattcggca gcagggccac acccgccgcg ataagcggtt
    14461 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    14521 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    14581 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    14641 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    14701 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    14761 tccagtagtt cgtgaacagt ttgcccggcg gcgcactgca tcggttgatc gttaaacagg
    14821 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    14881 ccactggctc gacttaccgt cgaacagtcg gagttctccc gcaggcgtct ctataccgct
    14941 taataactta atggcttcca gtgcctgcaa agtgcccatc accccgacca ccgggccaac
    15001 cacgcccgcc gtgcggcagt tgcgttctgg ctgctggtta tctggccaca ggcagcggta
    15061 acacccctgc tcccagggcg gtgtcagtac catcaactga ccgccaaatc cgaccgcgct
    15121 ggcggtgata agcggcgtgt tgagtgccac gcaggcggca ttaatctcct ggcgagtcgc
    15181 catattgtcg gtacagtcga gcaccacatc ggcttgtgca accgcatctt tcaacgcctc
    15241 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    15301 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga ggatttgtcg
    15361 ttgcagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    15421 ccctgccagg tatagcgcag caggtgtacc cagcccgcca agaccaataa tcagcacctg
    15481 gctgtcgagc agtttttgct gcccgtcaag agcgatatcg tcgagcagaa tttggcggct
    15541 ataacgcata aagtcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    15601 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgac gacggcgata ctgccgacac
    15661 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    15721 aatccgccag gcgctcaaca tgccgtgcca actgttccag cccctgcggt gcagaaggca
    15781 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    15841 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacgcc cagccgcagg cctgccgcgc
    15901 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    15961 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16021 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16081 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    16141 ccaccgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    16201 ttcctcctta tgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    16261 cattcccact tcaatggttt gcgcggcggc gtaatcgcgt acttcctggc tgattttcat
    16321 cgagcagaat tttggcccac acatggagca aaaatgggcg actttgccgg actcttgggg
    16381 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    16441 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    16501 atgccctttt gccaggtcag cagcgtgggc ggcaatctta taagtgataa gcccctgctt
    16561 aacatcttct ttattgggca gacccagatg ctcttttggc gttacgtaac agagcatcgc
    16621 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    16681 tgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    16741 ctcctcggtc atattgcggc ggatcatctg catcggcaca tggcctgggc cttcaatcat
    16801 cacctgtacg tcatattccc aggcgatttt ggtcagttcg cccagcgtat gcaattcggc
    16861 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    16921 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    16981 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17041 accggtcagg cgtttcgcgg tcatcggcac atagcgcagc aggacgcccg catggatagt
    17101 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    17161 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    17221 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    17281 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    17341 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    17401 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    17461 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gatgtgcgcc
    17521 aaagctcatt cccggatgct ggtggcgtaa aacctcgcta cggatgcgct cgcggcccat
    17581 attctcgcgg atggcgatga attccatttc cggcgtgatg atgccctggc gggcgtagtg
    17641 cagttgggtg acacggcgtc ctgttttggc gcgttttggc gttaataagc cgctaaaacg
    17701 tagctcgtcg agaccatcat ctgccagccg cgttctggtg taatcggaac tgcgcacggt
    17761 aagttcttcg gtatcggcgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    17821 ctgcacgtta atggcgatct gcggatcacc atacgggccg gaggtgtcgt agaccggaat
    17881 cgcttcgttt tcttcgtact gcggctgttc tttgctaccg ccaattagcg tcgggctaag
    17941 ctggatctca cgcatcggca cgcgcacgcc ggggtgtgtg ccagtgatat aaatgcgttt
    18001 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18061 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    18121 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    18181 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    18241 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    18301 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    18361 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    18421 aaaacctgtt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    18481 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    18541 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    18601 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    18661 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    18721 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    18781 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    18841 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    18901 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    18961 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19021 gcactacaaa tcggcgaatg gcagggagaa cctgtttggt taatacaaca gcagcggcgt
    19081 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgggctgtt tcaactggcc
    19141 ggacgtggcg tgcaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    19201 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    19261 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    19321 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    19381 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    19441 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    19501 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    19561 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    19621 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    19681 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    19741 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    19801 tatctaccgg aatataaagc cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    19861 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    19921 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    19981 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataaa
    20041 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    20101 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    20161 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    20221 tatgccgatc cgcaggcgct gcacgctctg ctcgataaac tggcgaaaag cgtcactttg
    20281 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    20341 ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    20401 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag tcacgctgtt taccaaaggc
    20461 ggcggacagt ggctggaagc catggctgaa accggttgcg atgcgctggg cctcgactgg
    20521 acaacggaca tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    20581 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    20641 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    20701 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacag
    20761 tatcaccgct aagagcctga tccatcaggc tattttattt gccattttga ggccgggcag
    20821 tgctcaaaat cctcacgtac tacgtgtacg ctccggtttt tccgcgctgt ccggcctcaa
    20881 actgccttca acaattacgc ctgttgaacc aggctctaag gagcgattat ggatctcgcg
    20941 tcattacgcg ctcaacaaat cgaactggct tcttctgtga tctgcgagga tcgactcgat
    21001 aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg
    21061 acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga gtataaagtt
    21121 gcccgcatcg ccaccaccat gccttacatt ccaggttttc tttccttccg cgaatatcct
    21181 gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt
    21241 catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggctt attggtggat
    21301 gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta aattcgaacc gctctccagc
    21361 gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg ggtctggcgc
    21421 agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag cgtggacagc
    21481 gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg
    21541 gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa
    21601 ttcaggtaaa ctgcggataa tttccgtatt tgagaactca acatgttaca aaacccaatt
    21661 catctgcgtc tggagcgcct ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc
    21721 gaacgcatgt accccaatta cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa
    21781 atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta
    21841 aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga cgatttcgat
    21901 ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg
    21961 cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc catcacgacc
    22021 gttgcgatgc tggaaatgac tcaggctggt cgcgaaatga gcgatgaaga gcttaaagaa
    22081 aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact tttagctgag
    22141 tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg tgagtcgggt
    22201 gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt taacgcctga
    22261 tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa
    22321 aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc cgtcgcactc
    22381 gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac tcaactgatt
    22441 gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc tctggagtcc
    22501 actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc
    22561 ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa
    22621 gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc actgaaagac
    22681 gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt ttcacccctt
    22741 ttgtctttct ggcgtcgatc attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc
    22801 cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca gggcgcggta
    22861 cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg
    22921 cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct cacgctcatc
    22981 aatatcaatg tttacagcaa accgccggtg aacattcgcg cgcgttttga cgatcgcggc
    23041 gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc taacgaccaa
    23101 atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc cttacgtcag
    23161 gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt gaccacctgc
    23221 gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat cgagcgccgc
    23281 cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa
    23341 ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa aacgttctga
    23401 tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtag cccatgccca
    23461 ttccggctcc gcgcggaata cccgcttcag ccatcgcgat atctcgtttc acccgtaact
    23521 catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt ttgctgctat
    23581 ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc agttgctgtt
    23641 gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct gtctgttgtt
    23701 cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga gcaaatgcag
    23761 atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc
    23821 gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt ccgtgccaac
    23881 gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat gactcgcctg
    23941 ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta cagcgcaggg
    24001 taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc aggcaaagaa
    24061 gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa gcgcgatcct
    24121 ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc gcgattatgg
    24181 gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc
    24241 tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc agcagcaggc
    24301 cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca cggatgaaca
    24361 aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt attcccctca
    24421 ggaaatgcag cagttacatc cgggagatga agaagcgtgg cggcggatcg atagcgcaga
    24481 cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg ctggaatgca
    24541 ccggatgcgc catatgcagc agtatgccgc gacaccacaa gcaattttca tcgctttcga
    24601 cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga ttattctctt
    24661 cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc gccgctatct
    24721 gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg tggcgctggg
    24781 gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga ttaaaggact
    24841 ggcgaaatac tttgccgagc gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt
    24901 gatggcgaaa gaggccgacc gtttaaaccg cgtggtaagc gagttgctgg aactggttaa
    24961 gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc actcattaca
    25021 gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg ccaacgacac
    25081 attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga atctctatct
    25141 caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggcca gcgaaagcgg
    25201 cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag atcagcttga
    25261 agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg ggctggcggt
    25321 cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc aggagggaaa
    25381 aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc cacaaggatg
    25441 acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg cactattttg
    25501 caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg
    25561 ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg aatggcggag
    25621 atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat tccggtgctg
    25681 attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac tggggcgctg
    25741 gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga aaaggcgctc
    25801 gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca gttcggtatg
    25861 gtcggtaaaa gcccggcgat gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg
    25921 gaagccacgg tgctgatcca cggcgattcc ggcaccggta aagaactggt cgctagggcg
    25981 attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg tgcgacactc
    26041 aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt tgctggagcc
    26101 gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa
    26161 attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa
    26221 gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat tgcggcgacc
    26281 catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct ctactatcgc
    26341 ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga tatccctctg
    26401 ctggctggcc attttcttca gcgctttgcc gagcgtaatc gcaaggcggt aaaaggcttt
    26461 acgccacagg tgatggattt gctgatccac tacgactggc cgggaaatat tcgtgaactg
    26521 gaaaacgcgg tggaacgggc ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag
    26581 ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga tattcagccg
    26641 ctggtggaag tggaaaaaga ggtgattctg gcggcgctgg agaaaacggg cggcaacaaa
    26701 accgaagccg cccgtcagtt agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt
    26761 tagttctgct cgcgttcgat ggcgcgccaa ccgatatctt tccggcagaa gcaatcatcc
    26821 cagtggatat cggtcattaa ggcgtaggcg cgtttctgcg cttctgccac ggtatgaccc
    26881 agcgcggtga cgcacagtac gcgcccgccg ctggttacca cctgctcgtc atccgccagt
    26941 tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag gccgtggatc
    27001 acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac gctgagagaa
    27061 gcacgttcat cccactctga cgttttctcg tccagtttgc cttcacaggc cgccaggcag
    27121 agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg atcgccaaag
    27181 cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag gcccgcatag
    27241 agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg ccagatgata
    27301 cgttccatgg tgcgctgatg aacttcatcg gttactaccg gcgcggggga gtaagcgccc
    27361 atcccgccgg tgtttggtcc gttatcttta tcgcctacgc gtttgtgatc ctggctggta
    27421 gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc ttcgccatcg
    27481 aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata
    27541 tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc tttcccggca
    27601 gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata caccagcgca
    27661 ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg ggccaggaaa
    27721 tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg gccgaagatt
    27781 ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc ttccgggccg
    27841 acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc
    27901 acgccgatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt acccggagca
    27961 acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc
    28021 ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg aagtggcgca
    28081 tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac
    28141 ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg gcatcaatac
    28201 cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact tccaggcctt
    28261 catcggcagc tttaataccg gcgattttcg cggagtacac gcggctcatc tggcccgcgc
    28321 caataccgat ggtcatattg tttttggcat agacgatagc attggatttc acgaatttcg
    28381 ccactttcca gcagaacagc gcatcacgca gttcctgttc ggtcggctga cgttgggtga
    28441 cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc
    28501 cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg caggtcagaa
    28561 cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa
    28621 taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca tccagctcgc
    28681 ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt
    28741 aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc ttcacaatca
    28801 cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta tcggcgatgt
    28861 tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag gcttctttca
    28921 cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct
    28981 gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accggcggct tctttgcttt
    29041 caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg tcgtaggcgg
    29101 cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt
    29161 cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc acgattgcga
    29221 catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata tcgatgttct
    29281 caaccgcatc ttccagcgag cagccttcac gagcaacagt ctgggcgaac ggatacaggt
    29341 taacaaccac catatcgata ggctggatct gatgttcttc cataatggca tcgtcctggc
    29401 cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg cgtccatcca
    29461 tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct ttttctgcta
    29521 acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa agtgcctggg
    29581 cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg actggacgac
    29641 gttgttgcat ggtaaatccc ctgggtttga ctattacaga gagcgttagc tgaatttttc
    29701 gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg cattgtaacg
    29761 aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca attttgacag
    29821 tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact ctgtgcgtaa
    29881 aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt ctgcaatttt
    29941 tctattgcgg cctgcggaga actccctata atgtgccttc atcgacacgg cggatgtgaa
    30001 tcacttcaca caaacagccg gttcggttga agagaaaaat cctgaaattc agggttgact
    30061 ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gcgctaaa
//
LOCUS       DADLSE010000037        29653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLSE010000037 DADLSE010000000
VERSION     DADLSE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.037"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN692_001647"
     CDS             complement(93..1064)
                     /gene="secF"
                     /locus_tag="JN692_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7263985.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFCKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN692_001648"
     CDS             complement(1075..2922)
                     /gene="secD"
                     /locus_tag="JN692_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7263986.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN692_001649"
     CDS             complement(2950..3282)
                     /gene="yajC"
                     /locus_tag="JN692_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7263987.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN692_001650"
     CDS             complement(3305..4432)
                     /gene="tgt"
                     /locus_tag="JN692_001650"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7263988.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN692_001651"
     CDS             complement(4487..5557)
                     /gene="queA"
                     /locus_tag="JN692_001651"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7263989.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIGRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            5650..6231
                     /gene="acpH"
                     /locus_tag="JN692_001652"
     CDS             5650..6231
                     /gene="acpH"
                     /locus_tag="JN692_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HAY7263990.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN692_001653"
     CDS             complement(6236..8053)
                     /gene="malZ"
                     /locus_tag="JN692_001653"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7263991.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGVLLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNAVQWQCKEGHGQLTDRILALPAISATVWMN"
     gene            complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN692_001654"
     CDS             complement(8209..9582)
                     /gene="proY"
                     /locus_tag="JN692_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7263992.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGVGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN692_001655"
     CDS             complement(9658..10977)
                     /gene="brnQ"
                     /locus_tag="JN692_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7263993.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN692_001656"
     CDS             complement(11384..12679)
                     /gene="phoR"
                     /locus_tag="JN692_001656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7263994.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN692_001657"
     CDS             complement(12737..13426)
                     /gene="phoB"
                     /locus_tag="JN692_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7263995.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            13616..14818
                     /gene="sbcD"
                     /locus_tag="JN692_001658"
     CDS             13616..14818
                     /gene="sbcD"
                     /locus_tag="JN692_001658"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7263996.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            14815..17958
                     /gene="sbcC"
                     /locus_tag="JN692_001659"
     CDS             14815..17958
                     /gene="sbcC"
                     /locus_tag="JN692_001659"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7263997.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            18084..19268
                     /gene="araJ"
                     /locus_tag="JN692_001660"
     CDS             18084..19268
                     /gene="araJ"
                     /locus_tag="JN692_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7263998.1"
                     /translation="MKKVILSLALGTFGLVMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN692_001661"
     CDS             complement(19411..20319)
                     /gene="mak"
                     /locus_tag="JN692_001661"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7263999.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            20444..21355
                     /gene="rdgC"
                     /locus_tag="JN692_001662"
     CDS             20444..21355
                     /gene="rdgC"
                     /locus_tag="JN692_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7264000.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(21433..21888)
                     /locus_tag="JN692_001663"
     CDS             complement(21433..21888)
                     /locus_tag="JN692_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264001.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRLIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(21885..22220)
                     /locus_tag="JN692_001664"
                     /pseudo
     CDS             complement(21885..22220)
                     /locus_tag="JN692_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN692_001665"
     CDS             complement(22375..22659)
                     /gene="ppnP"
                     /locus_tag="JN692_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7264002.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(22731..23408)
                     /locus_tag="JN692_001666"
     CDS             complement(22731..23408)
                     /locus_tag="JN692_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7264003.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIYAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN692_001667"
     CDS             complement(23666..23857)
                     /gene="yaiA"
                     /locus_tag="JN692_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7264004.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRVDHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN692_001668"
     CDS             complement(23907..24431)
                     /gene="aroL"
                     /locus_tag="JN692_001668"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7264005.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(24614..25072)
                     /locus_tag="JN692_001669"
     CDS             complement(24614..25072)
                     /locus_tag="JN692_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7264006.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            25192..26001
                     /gene="proC"
                     /locus_tag="JN692_001670"
     CDS             25192..26001
                     /gene="proC"
                     /locus_tag="JN692_001670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7264007.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN692_001671"
                     /gene_synonym="dgcC"
     CDS             complement(26018..27133)
                     /gene="adrA"
                     /locus_tag="JN692_001671"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7264008.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN692_001672"
                     /pseudo
     CDS             complement(27235..27555)
                     /gene="psiF"
                     /locus_tag="JN692_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
     gene            complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN692_001673"
                     /pseudo
     CDS             complement(27674..29089)
                     /gene="phoA"
                     /locus_tag="JN692_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN692_001674"
     CDS             complement(29190..29450)
                     /gene="iraP"
                     /locus_tag="JN692_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7264009.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGVLYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7417 a         8003 c         7309 g         6924 t
ORIGIN      
        1 gtttcccaag atgcggggat tttcgcaatc tccatacatc aaaaaaaatc ccgatcttct
       61 gaccgggatt ttcaacatca acgggaactt gattaaggca gaattgacgg ctgatccgcc
      121 ccttcttttt ccactttctg ctgcaacatg tgttcgcgct tcatacccag tttcagagcc
      181 aacgcagatg ctacgtagat ggaagatgca gtgccgatgg aaacaccgat aagcatggtc
      241 agcgagaagc cttccagtac cggaccaccg aagagataca gcatcaggat aaccatcaag
      301 gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg tcaaggacac gttaaagatt
      361 tcgtaaggcg taccgcgacg gatcttgcag aagttttcac gaattcggtc cgataccacg
      421 atactgtcgt taagcgagta accgataacc gacatcaacg atgccacaat ggtcaggtca
      481 atctcgatat ggaataacga caaaataccc agcgtaatga taacgtcgtg cgccagcgca
      541 ataaccaccc ctgccgccag tcgccactca aagcggaaac ctacgtacac gaggatagac
      601 agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa ggtctgcccc cacgctcgga
      661 ccgacgaact caatacgctt cactgctgca ttctgattgg tggattcgtt aatcaccttc
      721 agaacttggc tgcccagcac ctgaccgccg gtttcgcctt cagcaggcgg catacggacc
      781 atgatgtcgt ggctgctacc aaagttttgc agcattggct cttcaaaacc ggctttttgc
      841 aatgcatcac gcattacgtc aatttcagcc ggtttttcga gcgtaatttc gataaccgta
      901 ccaccggtga aatccagccc ccagttaaag ccgcgcacgc ccataataac gatagcagcg
      961 attaacagca gaccagagat gccgaaagcc cagtagtccc agcgcataaa gtcatagact
     1021 ttacggccgt ggtttagttg ttcaacagta tattcctgtg ccacatcgca ctcctcagat
     1081 tgacagcttc ttgacgcgct tgccgccata tagcaggttt acgatggcac gcgtaccgac
     1141 aatcgcggta aacatcgacg tcgccacacc gataccggta gtaatcgcga accctttaat
     1201 tgccccggta cccactgcgt acaggatgat gactttaatc agcgtggtga tgttcgcatc
     1261 gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt gcctgttgaa cagtacgtcc
     1321 gttgctcaac tcttctttaa tacgttcgtt gatcagtacg ttcgcatcga ccgctaccgc
     1381 aagggttaag acgatacccg cgatacctgg catactcagt gttgcgcctg gcaacagcga
     1441 cataataccg acgatcaaga tcaagttggc aatcagagca ctggtcgcaa tcagaccaaa
     1501 cttcttatag aagatgatca tgaacagaat agaaaccagc agaccggcaa ggcaagcctc
     1561 cagcccctgc tcaatgttct gcatacccag ggtcgggcca attgtacgtt cttcaacaat
     1621 ctgaatcggc gcgatcaacg caccggcacg cagcagcagt gacagctgac gggcttcgtt
     1681 cgggttgttg atgccggtga tacggaagct gttacccaga cgagactgga tgttggcgat
     1741 gttaatcacc tcttcctgtt tcaccagaac cgcacgacca tttgcatctt tcttaccgct
     1801 gtctttgtac tccacaaaca gggttgccat cggtttgccg atgttgtcct tggtgaagtt
     1861 agacataatg ttgccaccag cgctatcgag cgagatgtta acctgcggct ggttgtattc
     1921 gtcctggctg gaagtggagt cggtgatatg gtcaccagtc agaattacgc gtttgtacag
     1981 cacaaccggc tgaccttcgc gggtctgttt cacttcagag tcgcccggta cgcgaccgga
     2041 tgccgcagcg gcctgatcaa cattggtgtt taccagacgg aattccagcg ttgccgtcgc
     2101 gcccagaatc tctttcgcac gcgcagtgtc ctgaatacca ggcagttcaa caacgatacg
     2161 gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca agttggttta cacgattacg
     2221 caggatatta atgttctgct gcaccgcata ttcacgcgct tcactcagac gagcatcgct
     2281 cattaccgca cgcagctggt tgctgccctg gctgctaatc accaggtccg gatggcgctt
     2341 gctcagatac gcaatggctt catcacgagc tttagcatcg cggaaagtga tgctcaggcc
     2401 gtagttgttt tctttacgaa cagtggtata cgggatgccc ttttcgcgca ggtcactgcg
     2461 taggctatcg atattttgtt cctggagttt gccaagcgcg gtgtccatat ccacttccat
     2521 caggaagtga acgccgccac gcaggtcaag gccgagcttc atcggctcag catgaatagc
     2581 tgccagccag cgcggcgttg ccggggcaag gttaagcgcc acgacgtatt tgtcacccat
     2641 aacgcccatt aatgcttcac gagcgcgcaa ctgggtgtca gtggagtcga agcgcgcaag
     2701 aatagcgccc tcttccagtg ccacagactt agcagttatt ttttcttctt gtaacgtttt
     2761 ctggacctgg atcagcgttt gctcactggc ggcgactccg cgcgcaccag tgatctgaac
     2821 agccggatcc tcaccaaaca ggttgggaag cgcatacagc agaccgatga caatcaccac
     2881 gatcagcatg acgtacttcc acaaaggata acggtttaac acggcaattc ccttagggaa
     2941 aaattttaat tacagcgcct tcatggtgcc tttcggcagg acggcagcta cgaagtcacg
     3001 tttaataact acttcagtgg tgtcattcag cgcgatagca atgtagccgt tttccgctac
     3061 tttggttacg cgaccaacca ggccaccgtt cgtcagaact tcatcacctt tggcaatgga
     3121 gtccatcagc tttttgtgtt ctttggtgcg cttctgctgt ggacgcagga tcatgaaata
     3181 gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac atcgggctac cttgcgccgg
     3241 tgcacccgtt gccgctaccg catcagaaat aaaaaagctc attaaatttc cctcattatt
     3301 aatattaatc aacgttcaaa ggtggtactt ctcgcccctg acgctggtaa aaatcagtta
     3361 cgaagctctc taatttaccc tcttcaatag ccttgcgtaa acccgccatc aaacgctggt
     3421 agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa tatttcgttg caacggtcga
     3481 gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta gcaatcacac tcaggatcga
     3541 gtgggccagt atcgctctta tatttcgcat tgcggatttt caccacgcca tcggtcacga
     3601 acaaatgacc atttcgggcg ttgcgggttg gcattacgca gtcaaacata tcgataccgc
     3661 gacgtacgcc ttcaaccagg tcttccggtt taccaacgcc catcaggtaa cgcggtttgt
     3721 ctgccggaat ttgcggacac acatgctcca gaatacggtg catatctgct ttcggctcac
     3781 ccacagccag accgccgaca gcgtagccat caaagccgat atctaccaga cctttaacag
     3841 atatatcacg taaatcttcg taaatactgc cctgaattat accaaataac gcatttttgt
     3901 taccaagact gtcaaaacgc tcacggctac gcttcgccca acgcagagac atctccatag
     3961 agcgttttgc gtaatcccag tcagcaggat acggcgtaca ctcatcaaag atcatgacga
     4021 tatccgaacc aagatcgtac tgaatctcca tcgacttttc cgggtcgagg aaaatcgggt
     4081 cgccgttgat cgggttacgg aagtgaacgc cctgttcggt gattttacga atatcaccaa
     4141 ggctgaagac ctggaagccg ccggagtcgg taagaatcgg tcctttccac tgcataaaat
     4201 cgtgcagatc gccgtgcagt ttcatgattt cctggcccgg gcgcagccac aggtggaagg
     4261 tgttgccgag gataatttgc gcgccagtgg cttcaacttc ttccggcgtc atccctttta
     4321 cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc cactacgcca cgatcaaaga
     4381 ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag ttcaaatttc attttttctc
     4441 cactacgtca gaaaaacagt ccaacgttta aaccagcgcc gcggaattac tccccgacgc
     4501 gctcattaat tgcctgcgga ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc
     4561 gatatttctc ttctaccgcc gctttatagg cattcatggt gtgttgataa ccggcaaagg
     4621 ccgaaaccag cataatcagc gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga
     4681 ccactttgta ctggaagccc ggatagataa agatttgggt atcgtcaaag aacggttcga
     4741 taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga acgtactgaa gtggtgccaa
     4801 ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg
     4861 gtacttcagc gtattccgag tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct
     4921 ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat ctccacgcct ttggcgcgta
     4981 atttttccag caaaggctcg tcaaaatgca gaccagcggt cggggctgca accgcgcccg
     5041 gtttttcgct ataaacggtt tgataaagtt cgcggtcagc gtcttcgtcc ggacgaccga
     5101 tatacggcgg cagcggcata tggccgatgc tgttgaggat atccagtacc gagcgttcat
     5161 cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt cattgttgcg ttgatacttt
     5221 cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg
     5281 caagaatgcg tttgtcgtcg agcatccgct caaccagcac ttcaatcttg ccgccgctgg
     5341 ctttacgccc aaacagacgc gccgggatca cgcgggtatt attaaaaacc agaagatcgc
     5401 cggggttgag cttatcgagt aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc
     5461 cgtccagcga cagtaaacga cagctactgc gttcaggcat gggatagtgg gcaatcaggg
     5521 attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac actgactcag actaaaataa
     5581 gagtcgggta gtctagtgcc ggggcgctct ccctgcaacc tttacccccc tttggataga
     5641 atataatcga tgaatttttt agctcacctg catttagccc atctcgcgga aagctcgctt
     5701 tctggcaatt tactggctga tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac
     5761 gtcgtggctg gcattcatat gcatcgacgt atcgacgtat tgactgacaa tctgccggaa
     5821 gtccgcgaag cacgggagtg gtttcgtagt gaaacgcgcc gcgttgcgcc tattacgctg
     5881 gatgtcatgt gggatcactt tctttcccgc cactggtcgc agctgtcgcc ggactttccg
     5941 ctacaggaat ttgtctgtta tgcccgcgag caagtgatga cgattttgcc ggactcaccg
     6001 ccacgtttta tcaatctgaa caattacttg tggtcagagc agtggctggt gcgctatcgc
     6061 gatatggatt tcatccagaa cgtgttaaac ggcatggcaa gccgccgccc acgtctggat
     6121 gccctgcgtg actcctggta cgatttagac gctcattatg acgccctcga aacccgcttc
     6181 tggcagtttt atccgcggat gatggcgcag gcgtcacaca aggcgttata aaacgttagt
     6241 tcatccatac cgtagccgaa atggcaggca aagccagaat cctgtcagtc agttgcccat
     6301 gcccttcttt gcattgccat tgcacggcat tgagaaacgg tgacgcgggt agcaccactt
     6361 cacaggcctc gccacggttg attgccacca gtacacgttg ctgattcagc acgcggacaa
     6421 ataccactac gttatcttcc gcatacagca cctgacagcc gccacgacgc agcgcctgac
     6481 ttttcttacg cagcacaatc attcgccggt acagcgcgaa taacgccgta tcctgctttt
     6541 ccacctgcca ggggaacggt ttacggcaaa acggatcgtt tttaccatcc agtcccactt
     6601 catcaccgta ataaatgcac ggtacaccag gccaggtgaa cagccagacc accgccagcg
     6661 gcaggcgcgc aatatcccga ccgagcagcg ttttaaatcg cgcagtatcg tggctatcga
     6721 gctgattaaa catacgtaat tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc
     6781 aggccataca ggtttgggcg tcaatttgct gcggatcata agagatgtcg gtattggcaa
     6841 gaaatcccca caacgggaat gtgaagccac gatagttcat ggcagcatct tccacatcgg
     6901 cctgtaacca ttgccgtgca tcgccaaaat gttcgccgac aatatacgcg tccggctggg
     6961 tttctttcgc cgcttcggtg atcccggcaa cgtgctgcat attattgcgc gccccacccg
     7021 cctcccccag catatgcacc acatccagcc gccagccgtc catattccac ggcgctttca
     7081 gccagtggcg gacaatactg tcttccccgc gataaatttc attcaccaga ctttccgact
     7141 gataatccag cttcggcaag ctggcatagc caagccagtc gagcgccgtg ccatcatcac
     7201 taaacgagta ccagtcgcgc cagggcgatt cagagttgtg acaagctccc cccgtgccaa
     7261 gattgtgcct gtcaaaccag gcatgggaat cgccactgtg gttaaacacg ccgtccagca
     7321 ccagccgcat tcccagctgc tgcgtattgt gtcgcaaacg cagcaacacc ccatcaccgc
     7381 caaactgcgg atcgacatgg cgataatcct cggtatcgta tttatgtacg ctgggagctt
     7441 taaacaccgg attgagatac agcgctgtca cgccaagctt tttcagatac ggcagttttt
     7501 cgcttatccc gtccagatcg ccgccataga acgttgatcc gcccgcctgc gccgtgaccg
     7561 gttcatccca gtcacgcaag atgatctctt gtccggctgc atgatggtaa tagacatgat
     7621 cctgttcagc ttcacgagga agactacgcg caaaacgatc agggaagatc tgataaaaaa
     7681 tctgatccgc agcccattgt gggccgatat ccggtacatc gacggcaaac tgctccagtc
     7741 gtgccggcgg cattcggctg aagccctgcg gtgtaaacca acgctggcga tcgtgccaca
     7801 gcagtttgaa actgtaacgc cgccggggtt gtccgctgga gagatcaatc gccgcacgcc
     7861 atgcggtgac gcctggctgc ggctgactgc gctgcttatg cattggtact gacatttctt
     7921 cgttatcgtg ttctgtacgc agcataatgc gctgcggtgg gtcttcgccc gtcagccaca
     7981 gtgtaatgag cagttgatct ttgctttgtt taacaaatgg gggcaccggc aggtgccatg
     8041 catttaacat cataattccc ctgtgatgaa aatctgatca ccttgccata gcctaatgca
     8101 ggatcactca tcatctgaag gatatttggg ggaggaggac gggggcggat aactttcgga
     8161 atagccggat caggatttcc tcctgaaccg gcttacagaa cgcaagtatt attggttttc
     8221 agccagctga cgatcgtggc ggcgtttaaa catccagcca atcaacagca caacaatcca
     8281 cgcgaaaccg acatacagcg agatacgcgt atccgggtga taaccaatca acccgataat
     8341 aaagagcagg aaaatcagcc cgccgatggt cgttgctacc ccacccggca ctttaaattt
     8401 cagcgcctta acttcttctg gcggcaaacg gcgacggaag gcgatttgcg acagcaggat
     8461 cataatccac acccataccg tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt
     8521 ttccggcatg atgtagttca gatacaccgc aaacagcagc gcggtagtca tcaccagcac
     8581 cgtaacccac ggaataccgc gacgcgacgt tttgctgaaa attttcggtg cgctgccctg
     8641 ctctgccata ccgtggagca tacggcctac gccaaatacg tcactgttaa ttgccgacag
     8701 cgaagcggtc agcacaacaa agttaagaat gctggcggca aaggtaatgc ccatatgctg
     8761 gaacgtcagc acgaacgggc taccggcagt gccaacctga ttccacgggt agatagacat
     8821 aatgacgaac agcgtaccga cgtagaacac cagaatacgc atcggcacgg agttaatcgc
     8881 acgcggaatc gatttctcag gatctttcgc ttcaccggcg gtaatcccga taatttcgat
     8941 cccaccgtaa gcaaacatca ccatttgcaa cgacattacc atgccaagcc agccgttact
     9001 gaagaagccg ccgttgctcc acaggttatg aataccggtc ggttgcccgc cgttgccaac
     9061 cccccagatg atgatgccga aaccggcgac aatcatgatg atgatggtgg cgactttaaa
     9121 gaacgagaac cagaattcca gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca
     9181 aatgatcagc accacgctca gtacccaaat ccagtgcggc accgtcggga acaagacacc
     9241 catatagata ccaaaagcgg tcacatcggc gatggcgaca ataaggattt caaagcagta
     9301 ggtccatccg gtaatgtaac ctgccagcgg gccaaggttt tcctgcgcat aacgcgagaa
     9361 agagctggcg gccgggttat gtaccgacat ttcccccagc gcacgcataa tgatatacgc
     9421 cgcgatacca ccgataatat aggccaacaa cacgctcgga ccggccattt tgatggcgtc
     9481 tgccgaaccg taaaacagcc cggtgccaat tgctgaaccc agtgccataa agcgtatgtg
     9541 gcgggtactt agcccacgct ttagcttatt cttactttcc atcaattcct aacccatcaa
     9601 cacaataaaa aaccacgggg catcgcagcc ccgtggttaa aacaaatgtt cagtgattta
     9661 gtgagcgctg gaggtcacct gacgacctgc cgcgcgatcc cagataatgg ccagaaccac
     9721 catcaccact gttggcatta accacgccag accttgttcg gccagcggta aacgctgcgc
     9781 ccaggacggt aagatatcgc tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag
     9841 caggctgata aacatcggcg gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt
     9901 aaaacttaat acaaccagtg cgatacacgg cggataaata gcggtcagca ccggtacgga
     9961 gatctggatc agctggctta agccgaggtt agacaccacc atcgagaagc cgccgaggat
    10021 aaacaccagc gtacgataag agagcggtac gtactgggca aagaattctg cacaagcaca
    10081 ggtcaggcca accgccgtga ccagacaggc gatgaagatt aacgccgcca gcaggaagct
    10141 accgccgccg ccaaaggtgt gctgaacgta agcatgcaga atagcagcgc cgtttgcaga
    10201 ctgatcgacc agcgacgcgc tgtctgaacc cagacagaac agcgccaggt acagcaaagt
    10261 cagaccaaca cccgccatca ggccagccca gacggtataa cgggtcagca gacgcgcttc
    10321 ggtaacgcca cgagaacgcg ccgcgttaac aataacgata ccaaacacca ttgcgcccag
    10381 cgtatccatg gtcagatagc cgttaacgaa gccgttagaa aacgcagcgt tttgataagc
    10441 ctcagtcgcc gtgctgatag aacccgccgg ccagacaatc gcggcaacag acaggatgac
    10501 cagcgcgata attttcagcg gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg
    10561 atagagcgaa accagaataa cgatagcgaa atagaccagg ctgtagataa acagcggcag
    10621 cgcggaatca cccgtcagcg gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg
    10681 cgtagcgaaa agcggcccca ccgccaggta acaaaccgtt gccagcagta cgccagcgac
    10741 tttaccgatt ggcgtgctga ggctgtcaac accgccgcca acttttgcca gcgccactac
    10801 cgttaatacc ggcaggccaa cggcagtaat gaggaagccg aatgccgcag tccagacgtg
    10861 ttcgcctgcc tgtaagccga ccattggagg gaaaataatg ttacctgcgc cgacgaacaa
    10921 cgcaaatgtc ataaagccca gagcgatgat atcgcgcgat cttaattgat gggtcataaa
    10981 tcatactgcc tgtggatgtg gtgttgtaac gttaaatttt tgtgccaatc cctggggatg
    11041 atacagcggt atatttaaac aattttagcg ggaaatactc ccaaccaggc gcggcgaaga
    11101 atagtttttc gatttaccgc gttaatgcag tcagtatgac agcacctgaa gcgcaattta
    11161 aacgcttata acgttttaaa gcaaggaggg atctaaaaac cagaggtata tagcgataag
    11221 aaatcaccaa ccagtataaa acactatttc cgtgaaaaag acatggctaa tcatgcgaac
    11281 aaaaaatcac caaccgttgt aaaatctaac gcccgtattt tgactgcaaa gaaaataagc
    11341 cagcatcggc tggcttatgg caataaaaga tgacaaaggc ggattaatcg ctgtttttgg
    11401 caattaaacg ttccgggata acaaaactga aacgtgttcc ttttcctact gtactctcaa
    11461 tattcaggcg actttcgtga tgattcaccg catgtttcac gatcgctaac cctaatccgc
    11521 taccgccggt ttgccgggaa cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg
    11581 gaatatgctc cggtgcaatg cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg
    11641 gcactcgctg ccagcgtacg gtgatatgcg tgccttccgg cgtatgattc acggcgttat
    11701 agaccaggtt cgaaatcgca ctgcgtagct gatcttcgtt gccagacacc ttgaggccgt
    11761 tatctatctc aaaggtaaat gtctgttttt tctgactcag agtctgagcc tcgcgctcaa
    11821 caacgcgcag catcatcggc acatcaacct tttcattgag caattgcgtc ggcgcggctt
    11881 caattttcga cagcgttagc aactgcttca ccagcccttc catccgctgg gtctgctcgc
    11941 gcatggtgtg caacgctttt tcgcgtaccg cgccttccag cggctgctca tccatcatct
    12001 ccaggtaacc ctgtaacacg gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa
    12061 agttacgccg cgccccttcc agttgatgca tttgcgtgac atcacgcgcc accatcagca
    12121 actgtttgtg ggtataaggc atgacgcgaa tttccagatg ccgcccggtg ttgagcacca
    12181 gattgagcgg gcgagaaaaa tcacgcgttt tcagatattg cgtaaactcc gggtaacgca
    12241 gtaggttaag gatgttctgc ccgttatctt ccggccagcg caaaccaaga atttgttgcg
    12301 ccagaccgtt acaccagaaa ataccgccct cttccgtggt cagcaccacc gcgtcgggca
    12361 gcgactccgc gccgctacga aagcgtttaa tcagattgcc cagttcacgg cggcgttttt
    12421 tatttcgcag ctgcatctgg tgtaagccgt atagtagcgg ttcccagcta ccacgccccg
    12481 gtggcggggt catactgcga tccacccaca gccaccatga aaggcgcaat aaattccaga
    12541 aatgccagat aagcagtcct gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa
    12601 atgcacccag gatgaaagcc gggaggcagc aaagtagcag ctccagcacc agccttttcc
    12661 acgacagccg ttccagcacg taagatactc cagttaagaa atcataagcc ctgctctgcg
    12721 tccgatgagc aaggcgttaa aagcgggttg aaaaacgata tcctgtaccg cgcacggtct
    12781 gcaccatgcg gtcatgcccg ccgggctcca gtgctttacg caggcgacga atgtggacat
    12841 cgaccgtgcg gtcttccaca tacacgttag ttccccagac gtggtttaac agctgctcgc
    12901 ggctgtacac gcgctcagga tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc
    12961 ccatctccag cggctcttcg cccgccatca ctcggtgaga tgtcgggtcg agacttaatc
    13021 cctgcatctc aatcacctct tccaccgcca ttggcgaaat acggcgcatt accgctttga
    13081 ttcgcgccac cagctccttc ggcgaaaatg gcttggtgat atagtcatcc gcgccggttt
    13141 caaggccgcg cacgcgatct tcttcttccc ctctggcggt caacatcacc actggaatat
    13201 cccgggtcat cgactcgcgc ttgaggtgtt tgatgaactg gataccggag ccgccaggta
    13261 acatccagtc aaggagaatt aaatccggcc agggttcatt cagttgattc acagcactgt
    13321 cataatcttc cgcttcgacc ggctgaaagc cattttgttc gagcacgaag cagaccattt
    13381 cgcgaattgg agcttcatct tctacgacca gaatacgtct cgccatgatt tgccctgttg
    13441 taataaatag gttgcgatca ttaatgcgac gtcattatgc gtcagattta tgacagattt
    13501 atgaaaagct cgtcgcacat atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa
    13561 atggcacatc tgtttgggta taatcgcgac catgcttttt cgccagggaa ccgttatgcg
    13621 catccttcac acctcagact ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc
    13681 tgaacatcag gcttttcttg actggctgct ggagacagca caaacccatc aggtggatgc
    13741 gattattgtt gctggtgatg ttttcgatac cggctcgccg cccagttacg cccgcacgtt
    13801 atacaaccgt tttgttgtca atttacagca aactggctgt catctggtgg tactggcagg
    13861 aaaccatgac tcggtcgcca cgctgaatga atcgcgcgat atcatggcgt tcctcaatac
    13921 aaccgtggtc gccagcgccg gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc
    13981 aggcgcagtg ctgtgcccca ttccgttttt acgcccgcgt gacattattt ccagccaggc
    14041 ggggcttaac ggtattgaaa aacagcagca tttactggca gcgattaccg attattacca
    14101 acaacactat gccgatgcct gcaaactgcg cggcgatcag cctctgccca tcatcgccac
    14161 gggacattta acgaccgtgg gtgccagtaa aagtgacgcc gtgcgtgaca tttatattgg
    14221 cacgctggac gcgtttccgg cacaaaactt tccaccagcc gactacatcg cgctcgggca
    14281 tattcaccgc gcacagatta ttggcggcat ggaacatgtt cgctattgcg gttcccccat
    14341 tccactgagt tttgatgaat gcggtaagag taaatatgtc catctggtga cattttcaaa
    14401 cggcaaatta gagagcgtgg aaaacctgaa cgtaccggta actcaaccca tggcggtgct
    14461 gaaaggcgat ctggcgtcga ttaccgcaca gctggaacag tggcgcgatg tatcgcagga
    14521 gccacctgtc tggctggata tcgaaatcac tactgatgag tatctgcatg atattcagcg
    14581 caaaatccag gcattaaccg aatcattgcc tgtcgaagta ttgctggtac gtcggagtcg
    14641 tgaacagcgc gagcgtgtgt tagccagcca acagcgtgaa accctcagcg aactcagcgt
    14701 cgaagaggtg ttcaatcgcc gtctggcact ggaagaactg gatgaatcgc agcagcaacg
    14761 tctgcagcat cttttcacca cgacgttgca taccctcgcc ggagaacacg aagcatgaaa
    14821 attctcagcc tgcgcctgaa aaacctgaac tcattaaaag gcgaatggaa gattgatttc
    14881 acccgcgagc cgttcgccag caacgggctg tttgccatta ccggcccaac cggtgcgggg
    14941 aaaaccaccc tgctggacgc catttgtctg gcgctgtatc acgaaactcc gcgtctctct
    15001 aacgtttcac aatcgcaaaa tgatctcatg acccgcgata ccgctgaatg tctggcggag
    15061 gtggagtttg aagtgaaagg tgaagcgtat cgcgcgttct ggagccagaa tcgggcgcgt
    15121 aaccagccgg acggtaattt gcaggtgcct cgcgtagagc tggcgcgctg cgccgacggc
    15181 aaaattctcg ccgacaaagt gaaagataag ctggaactga cagcgacgtt aaccgggctg
    15241 gattacgggc gcttcacccg ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg
    15301 aatgccaaac ccaaagaacg cgcggaattg ctcgaggagt taaccggcac tgaaatctac
    15361 gggcaaatct cggcgatggt ttttgagcag cacaaatcgg cccgcactga gctggagaag
    15421 ctgcaagcgc aggccagcgg cgtcacgttg ctcacgccgg aacaagtgca atcgctgaca
    15481 gcgagtttgc aggtacttac tgacgaagaa aaacagttaa ttaccgcgca gcagcaagaa
    15541 caacaatcgc taaactggtt aacgcgtcag gacgaattgc agcaagaagc cagccgccgt
    15601 cagcaggcct tgcaacaggc gttagccgaa gaagaaaaag cgcaacctca actggcggcg
    15661 cttagtctgg cacaaccggc acgaaatctt cgtccacact gggaacgcat cgcagaacac
    15721 agcgcggcgc tggcgcatat tcgccagcag attgaagaag taaatactcg cttacagagc
    15781 acaatggcgc ttcgcgcgag cattcgccac cacgcggcga agcagtcagc agaattacag
    15841 cagcagcaac aaagcctgaa tacctggtta caggaacacg accgcttccg tcagtggaac
    15901 aacgaactgg cgggttggcg tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg
    15961 cggcaatggc agcaacagtt aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc
    16021 acgttgacgt taaccgccga tgaagttgct accgccctgg cgcaacatgc cgagcaacgc
    16081 ccactgcgtc agcgcctggt cgcgctgcat gggcagattg ttccccaaca aaaacgtctg
    16141 gcgcagttaa tggtcactat ccagaatgtc actctggaac agacgcaacg taatgtcgca
    16201 cttaacgaaa tgcgccagcg ttataaagaa aagacgcagc aacttgccga tgtgaaaacc
    16261 atttgcgagc aggaagcgcg catcaaaacg ctggaagccc agcgtgcaca gttacaggcg
    16321 ggtcagcctt gtccactttg tggttccacc agccacccgg cggtcgaggc gtatcaggcg
    16381 ctggagcctg gtgttaatca gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc
    16441 ggcgaagaag gtgcggcgct gcgcgggcaa ctggatgcat taacgaagca gcttcagcgc
    16501 gatgaaaacg aagcgcaaag cctccgacaa gatgagcaag cacttactca acaatggcaa
    16561 gccgtcacgg ccagcctcaa tatcaccttg cagccacagg acgatattca accgtggctg
    16621 gatgcacaag atgagcacga acgccagctg cggttactca gccaacgaca tgaattacaa
    16681 gggcagattg ccgcgcataa tcagcaaatt atccagtatc aacagcaaat tgaacaacgt
    16741 cagcagcagc tcttaacggc attggcgggt tatgcactga cattgccaca ggaagatgaa
    16801 gaagagagct ggttggcgac acgtcagcaa gaagcgcaga gctggcagca acgccagaac
    16861 gaattaacca cgctgcaaaa ccgtattcag cagctgacgc cgattctgga aacgttgccg
    16921 caaagtgatg atctcccgca cagcgaagaa actgtggcgc tggataactg gcggcaggtt
    16981 catgaacaat gtctcgcatt acacagccag cagcagacgt tacagcaaca ggatgttctg
    17041 gcggcgcaaa gtctgcaaaa agcccaagcg cagtttgaca ccgcgctaca ggccagcgtc
    17101 tttgacgatc agcaggcgtt ccttgcggcg ctaatggatg aacaaacact aacgcagctg
    17161 gaacagctca agcagaatct ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag
    17221 acagcagaaa cgctggcaca gcatcaacaa caccgacctg acgggttggc tctcactgtg
    17281 acggtggagc agattcagca agagttagcg caaactcacc aaaagttgcg tgaaaacacc
    17341 acgagtcaag gcgagattcg ccagcagctg aagcaggatg cagataaccg tcagcaacaa
    17401 caaaccttaa tgcagcaaat tgctcaaatg acgcagcagg ttgaggactg gggatatctg
    17461 aattcgctaa taggttccaa agagggcgat aaattccgca agtttgccca ggggctgacg
    17521 ctggataatt tagtccatct cgctaatcag caacttaccc ggctgcacgg gcgctatctg
    17581 ttacagcgca aagccagcga ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat
    17641 gcggtacgcg atacccgtac cctttccggc ggcgaaagtt tcctcgttag tctggcgctg
    17701 gcgctggcgc tttcggatct ggtcagccat aaaacacgta ttgactcgct gttccttgat
    17761 gaaggttttg gcacgctgga tagcgaaacg ctggataccg cccttgatgc gctggatgcc
    17821 ctgaacgcca gtggcaaaac catcggtgtg attagccacg tagaagcgat gaaagagcgt
    17881 attccggtgc agatcaaagt gaaaaagatc aacggcctgg gctacagcaa actggaaagt
    17941 acgtttgcag tgaaataact attcagcagg ataatgaata cagaggggcg aattatctct
    18001 tagccttgct ggtcgttatc ctgcaagcta tcactttatt ggctacggtg attggtagcc
    18061 gttctggtgg ttgtgatggt ggtatgaaaa aagtcatttt atctttggct ctgggcacgt
    18121 ttggtttggt gatggccgaa tttggcatta tgggcgtgct cacggagctg gcgcataacg
    18181 taggaatttc gattcctgct gccgggcata tgatctcgta ttatgcactg ggggtggtgg
    18241 tcggtgcgcc aatcatcgca ctcttttcca gccgctactc actcaaacat atcttgttgt
    18301 ttctggtggc gttgtgcgtc attggcaacg ccatgttcac gctctcttcg tcttacctga
    18361 tgctcgccat tggtcggctg gtatccggct ttccgcatgg cgcatttttt ggcgtcggag
    18421 cgatcgtgtt atcaaaaatt atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg
    18481 tttccgggat gacagtcgcc aatttgctgg gcattccgct gggaacgtat ttaagtcagg
    18541 aatttagctg gcgttacacc tttttattga tcgctgtttt taatattgcg gtgatggcat
    18601 cggtctattt ttgggtgcca gatattcgcg acgaggcgaa aggaaatctg cgcgaacaat
    18661 ttcacttttt gcgcagcccg gccccgtggt taattttcgc cgccacgatg tttggcaacg
    18721 caggtgtgtt tgcctggttc agctacgtaa agccatacat gatgtttatt tccggttttt
    18781 cggaaacggc gatgaccttt attatgatgt tagttgggct agggatggtg ctgggaaata
    18841 tgctaagtgg caggatttca ggacgttatt caccactgcg cattgcagca gtgactgact
    18901 ttatcattgt actggcactg ctgatgctct ttttctgcgg cggcatgaaa acaacgtcgc
    18961 ttatttttgc ttttatttgt tgcgcgggat tatttgccct ttcagcacct ctgcaaatat
    19021 tgttactgca aaatgccaaa ggcggagagt tattaggtgc cgcaggtggg caaatagcgt
    19081 ttaacctcgg tagcgccgtc ggcgcatatt gcggaggtat gatgctgacg ctggggctgg
    19141 catataatta cgtggcgctg cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc
    19201 tgctgtatgg tcgctataag cgccagcaag cggcggatag tccggtgctg gcgaaaccac
    19261 tggggtagat tactgtcacg gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta
    19321 tccgccctac gcggttctgg cacattttgt aggcctgata agacgcggca agcgtcgcat
    19381 caggcatgat gcgccaattg cctacgtttt ttactcttgt ggccataacc acgcagcgcc
    19441 gcgtacgccg ctggaatcac cgtgcttcgc cttacgcacc ggcgtttcac attcgccgcc
    19501 gaagacaaat tgtttaatca actgcccaac cgtttgatat aaacggtcta cattgctcat
    19561 cccgcccccc aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt
    19621 tgccagccgc agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac
    19681 caggcggata atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccgtcgc
    19741 gaatcccgtg cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac
    19801 ttcctcgcga taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc
    19861 acctgccgtg ccattgccgc cgatatgcgt ccgcccattg aatgccacgc ccgcgccgca
    19921 tcccgtgccg ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc
    19981 ttctgaaacc gccagacagt tggcatcatt tgccagccgc acttcccgct gcaacctcgc
    20041 gcttaagtct ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac
    20101 accggtgtag ggcgaaatcg aacctggaat gcccatacct accgttccgc gctgccctgt
    20161 cgcctgctcc gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc
    20221 acgcggcgtg ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac
    20281 ttcagttttg gtgccgccta aatcgatacc tatacgcact gtactctcct tatttttttc
    20341 aatatcaata gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt
    20401 tatcatgccc gctaaattta acgacaaggc cgtggaaatt atcatgctgt ggttcaaaaa
    20461 tttaatggtt taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca
    20521 gctagcctcg atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt
    20581 tcctccgatg ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat
    20641 ctgcgcgcgc aaagaagaaa aaatcctccc gtctccggtg attaaacagg cgctggaagc
    20701 gaaaatcgcc aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc
    20761 gctgaaagac gaagtgctgc actctctgct gccgcgtgct ttcagccgtt ttagccagac
    20821 aatgatgtgg ctcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa
    20881 agcggaagat acgctggcat tactgcgtaa aagcctgggg tcgttaccgg ttgtaccatt
    20941 gagcatggaa aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagtgcggc
    21001 acagggcttc cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt
    21061 gatccgcgcg aagaaacaag atctgaccag cgaagagatc accaatcaca ttgaagccgg
    21121 aaaagtggtg actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga
    21181 cgatggttcg ctcaagcgtc ttaagttctg cgacgagctg cgcgatcaaa acgaagatat
    21241 cgaccgtgaa gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact
    21301 ggcagcgtta attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct
    21361 gattttcctt taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca
    21421 ccaggcatat aattattcgc tacggcgagc aataattttt agcgcagcaa tattatgcgt
    21481 tttacgccgt aactgatcta atcgcctcat ccctattagc ctgtcagcag cacgcagaat
    21541 attcactaac ccccatttat cgctggtaaa ttgttttgtt aacagctccg cttttggggt
    21601 atcaatatat tcacgcaata aatcagaaat tgaacaaata tccgtcaggt aaggatacca
    21661 gcaagtttca ccgtgaaaat tacgcactcc agcgttacct gcaataaagt ctttcgggta
    21721 atcccagatc acgtttttat ccaacgtgat ccaataacgc ggtaaatcgg tacttccacc
    21781 attttggctg cgcatgggat aacgggtaca gtgaatttgc aggttgatcg ttggcgtcag
    21841 taagtcatag atctcccgct gtaacttgct ccatggacgt tgtgtcattg tttttcctca
    21901 agccagtgac gcgcttcact gcgaattttc cgcgttgccc cggttttcac taaatcacgt
    21961 aactgcgaaa gatcgggttg ttcgacaaat aagagtaatg atggttcctc ttttaaccac
    22021 gccgttttca catcggcagc cagctgtgga ttattaatag ccagcgaccg atcctgaggc
    22081 tcagtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc cgtttggcgc
    22141 aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga tttatctttt
    22201 atcgtagcca gttccgcaaa aaatacctgg agatcttcac tgatatttat atcaaccttg
    22261 atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat tattatgggt
    22321 taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga ggaattacag
    22381 atagcggcac agataagagg tgggttcggc aacttgaaga tgaaactcac tgtgaccggg
    22441 aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag gcagtaacac
    22501 attcagcgcg ccactgatta ccgtcatctc ttccggctca gcggtgctga aggtgtattc
    22561 gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa agccgatcga
    22621 tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct gtctggtagt
    22681 tgaacatcta cttcactata ggggccagag gcgctggctg tcacgcaaaa ttacaccagt
    22741 aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac atcgagctgt
    22801 ttttgcagta aatcacgatg acgttggtga aatcctaaac aatccagcat gatgacatca
    22861 gccccttttg ccagtaattc tttcccagca taaatgattt tttgttctga atcatgaatg
    22921 gggttaccca atgaaaatac cggcgatttc tgcaaaattt gccatttttg cgcctgaacg
    22981 ggcagcatct cctcaaccgg aacgataacc cccacctgat gatcttcaac aatagaggaa
    23041 accagtggag gcaatattcg cgacggctca agaaagatcg tattacgcgc agtcatacta
    23101 ctaatgtttg ctgtactcat taataaaatg acgtcataac cctgattatc gagcacttca
    23161 accacacctt gcagatctcg ctccacttta cgacgcgaaa catgggccag ctggttgtca
    23221 tttaataatg tgagaatggt gtcttcgcct gcttctggcg cgtactccgc catcacttct
    23281 tcacgactta acttccccag caggctatga tgggaaatat tatcttcgtc aatgtattcc
    23341 gtcaggagcg gcaaaacctc ctgcataggt acaatgccga tggtcaggat cgccaacgac
    23401 gcactcattg ttcatccacc ttacttttct ttcgtcgtta attaccgggc aagtgtgaag
    23461 caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc agacgtgcac
    23521 ggcataatgt aaagattcca aatgattcca gtaatggaat gttattgttt aatattctaa
    23581 ttattagaaa aacatgaatt atgaaaaatg tgacgcagat cccttgtagt ggcactcaga
    23641 atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag cgttttttcg
    23701 catccatcgc ttcctgagcc gtcggatgtt cactgatcaa cgagtctggt ttaggatgat
    23761 cgactctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca atcgtcacta
    23821 tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat ttcacgggat
    23881 gaacgttaag tataggcgct ctaaaatcaa caattgatcg tctgtgccag ggcgctacga
    23941 atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg cgcaacttcg
    24001 cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag cggttttccc
    24061 gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac caggactgat
    24121 actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg attaaattcc
    24181 gtcagaataa tgccgccgcc tgtagcgata acggtggatg gcgcagttac cgcttccagc
    24241 gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat ctccgcgacc
    24301 gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg gttaagcgaa
    24361 tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat cagaaaaaga
    24421 ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc ggtgtcatga
    24481 gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa attccactta
    24541 aagtgaaaat ctcaatacat cccttactat accaataaat attcaagaat gaagtgtaaa
    24601 taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc tccagctccg
    24661 cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc tggatcccac
    24721 tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg gtcgctggcg
    24781 tgtaacgttc gccgcgcgga ttaagcgccg ctgcgccttt ctcgatggct tcagcagcca
    24841 aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca atttcgttat
    24901 cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat ggcggcacgc
    24961 gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc gcgcgataca
    25021 aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc ataaagtcat
    25081 cctttgttgg gtaatcctct attgtgtcgc gcttttgcct tccggcatag ttctgtttat
    25141 gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc aatggaaaag
    25201 aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg tctgattgcc
    25261 agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga taaagtcgcc
    25321 gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt ggcgcaaatc
    25381 gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct tagcgaaatc
    25441 acctccagcc tgaataaaga ctctctggtc gtttctattg ctgcaggtgt cacgctcgac
    25501 cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc gaacactccc
    25561 gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac cccagaagat
    25621 accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat tgctgagccg
    25681 atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtatt tatgtttatc
    25741 gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc gtataaattt
    25801 gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga acatccgggg
    25861 gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt acgcgtactg
    25921 gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat ggaaaaatca
    25981 gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact tcggtgcggt
    26041 tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac cactcacgat
    26101 agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaaa gttacctgtg
    26161 gcgtattcgg caagcgtaac gtgtttagcc cttcacgcac ccgtaacatg gcggtaatgg
    26221 cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca aaccgaccaa
    26281 tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat
    26341 cgcccacatc atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca
    26401 gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt
    26461 cccaatgacg tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc
    26521 tgcgtttatg ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag
    26581 gataaacgac aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca
    26641 cggtaatgcc cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg
    26701 cgacaaacag acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag
    26761 cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat
    26821 cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc
    26881 aagctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg
    26941 gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac
    27001 ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag
    27061 ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta
    27121 tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag
    27181 tatgaaattt cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc
    27241 ggctttcttg aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg
    27301 cgttgcttga ttattacatt cgcgcatctt ttgctactgt ggcgtcaaac ttttttcacc
    27361 aggcgcagac ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt
    27421 caacgcctgc gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa
    27481 ggttctctcg gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag
    27541 taatgttatt ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt
    27601 gatctgccat taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact
    27661 gccgggcgcg gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct
    27721 ggtcggtcag tccaacaaca ttggcggcat gcgggccata cgccgcaata cgcaactgac
    27781 tgccggtatg ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc
    27841 catctttagt atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct
    27901 ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga
    27961 attccagcgc ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag
    28021 gattcgcagc gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt
    28081 tctcattttt actcaacaat tcaatggctt tgtcggtcat ctgtgccagg gttggtacac
    28141 tgtcattacg ttgcggatta ggcgtatagg tgactgcggg cttatcgata ttgccgtggt
    28201 acgttgcttt cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca
    28261 ggggtttttg ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact
    28321 gataaccacg cgcctgtgcc tattcacgca gcgtttttcc ctgccattca ccagcggttg
    28381 ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca
    28441 gctgttcggt aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac
    28501 tggtcgcgct cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg
    28561 tggcatcctg caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca
    28621 tttccagaat cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg
    28681 ttttgacacc ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt
    28741 tgccagtttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga
    28801 tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat
    28861 cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat
    28921 cacgcagagc ggcggtctga tcacccgtta agcggcgagc accgccgggt gcagtaatat
    28981 cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag
    29041 gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc
    29101 catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact
    29161 ttatgacagc tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt
    29221 acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg
    29281 tacagcaccc cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga
    29341 agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct
    29401 tcttcttttt gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt
    29461 ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt
    29521 attattttta tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa
    29581 acgtttctct tatttaaata attacccaca caaaatatag gcttttaatt atcaaccttt
    29641 attataaata aaa
//
LOCUS       DADLSE010000038        29573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLSE010000038 DADLSE010000000
VERSION     DADLSE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.038"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..427
                     /locus_tag="JN692_001675"
     CDS             <1..427
                     /locus_tag="JN692_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7264010.1"
                     /translation="VNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(459..1295)
                     /locus_tag="JN692_001676"
     CDS             complement(459..1295)
                     /locus_tag="JN692_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY7264011.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            1553..2221
                     /gene="folE"
                     /locus_tag="JN692_001677"
     CDS             1553..2221
                     /gene="folE"
                     /locus_tag="JN692_001677"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7264012.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            2238..3395
                     /locus_tag="JN692_001678"
     CDS             2238..3395
                     /locus_tag="JN692_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7264013.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            3537..4577
                     /gene="galS"
                     /locus_tag="JN692_001679"
     CDS             3537..4577
                     /gene="galS"
                     /locus_tag="JN692_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7264014.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            4857..5855
                     /gene="mglB"
                     /locus_tag="JN692_001680"
                     /pseudo
     CDS             4857..5855
                     /gene="mglB"
                     /locus_tag="JN692_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
     gene            5916..7436
                     /gene="mglA"
                     /locus_tag="JN692_001681"
     CDS             5916..7436
                     /gene="mglA"
                     /locus_tag="JN692_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7264015.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            7452..8462
                     /gene="mglC"
                     /locus_tag="JN692_001682"
     CDS             7452..8462
                     /gene="mglC"
                     /locus_tag="JN692_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7264016.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN692_001683"
     CDS             complement(8533..9768)
                     /gene="preA"
                     /locus_tag="JN692_001683"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7264017.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(9762..11000)
                     /locus_tag="JN692_001684"
     CDS             complement(9762..11000)
                     /locus_tag="JN692_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7264018.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(11194..11433)
                     /locus_tag="JN692_001685"
     CDS             complement(11194..11433)
                     /locus_tag="JN692_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7264019.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN692_001686"
     CDS             complement(11436..12155)
                     /gene="sanA"
                     /locus_tag="JN692_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7264020.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN692_001687"
     CDS             complement(12305..13189)
                     /gene="cdd"
                     /locus_tag="JN692_001687"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7264021.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(13319..14014)
                     /locus_tag="JN692_001688"
     CDS             complement(13319..14014)
                     /locus_tag="JN692_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7264022.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(14011..14409)
                     /locus_tag="JN692_001689"
     CDS             complement(14011..14409)
                     /locus_tag="JN692_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7264023.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYILIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            14648..15595
                     /gene="dusC"
                     /locus_tag="JN692_001690"
                     /pseudo
     CDS             14648..15595
                     /gene="dusC"
                     /locus_tag="JN692_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN692_001691"
     CDS             complement(15966..16049)
                     /gene="yohP"
                     /locus_tag="JN692_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HAY7264024.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN692_001692"
     CDS             16183..17709
                     /gene="mdtQ"
                     /locus_tag="JN692_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7264025.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            17762..18523
                     /locus_tag="JN692_001693"
                     /pseudo
     CDS             17762..18523
                     /locus_tag="JN692_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(18640..19218)
                     /locus_tag="JN692_001694"
     CDS             complement(18640..19218)
                     /locus_tag="JN692_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7264026.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            19388..19975
                     /locus_tag="JN692_001695"
     CDS             19388..19975
                     /locus_tag="JN692_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7264027.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVQEVLLALTVILWGYGYRLF"
     gene            20149..21081
                     /gene="pbpG"
                     /locus_tag="JN692_001696"
     CDS             20149..21081
                     /gene="pbpG"
                     /locus_tag="JN692_001696"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7264028.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN692_001697"
     CDS             complement(21119..22834)
                     /gene="dld"
                     /locus_tag="JN692_001697"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7264029.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            23030..25327
                     /gene="bglX"
                     /locus_tag="JN692_001698"
     CDS             23030..25327
                     /gene="bglX"
                     /locus_tag="JN692_001698"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7264030.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            25579..26496
                     /gene="osmF"
                     /locus_tag="JN692_001699"
     CDS             25579..26496
                     /gene="osmF"
                     /locus_tag="JN692_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HAY7264031.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            26503..27660
                     /gene="yehY"
                     /locus_tag="JN692_001700"
     CDS             26503..27660
                     /gene="yehY"
                     /locus_tag="JN692_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HAY7264032.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            27653..28579
                     /gene="yehX"
                     /locus_tag="JN692_001701"
     CDS             27653..28579
                     /gene="yehX"
                     /locus_tag="JN692_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HAY7264033.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            28584..29315
                     /gene="yehW"
                     /locus_tag="JN692_001702"
     CDS             28584..29315
                     /gene="yehW"
                     /locus_tag="JN692_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7264034.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQATLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(29296..29403)
                     /locus_tag="JN692_001703"
     CDS             complement(29296..29403)
                     /locus_tag="JN692_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7264035.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
BASE COUNT         7119 a         7384 c         7941 g         7129 t
ORIGIN      
        1 cgttaacaaa gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga
       61 atggaaactg tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa
      121 caaaccgcta tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct
      181 ggcgctggaa gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga
      241 cagcgcgacg gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca
      301 ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag
      361 tcgtgacgac tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg
      421 cttctgatga gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgtgca
      481 tggaagcgca ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc
      541 ggctgaatac gcagcgtcat cggccacgct ttctggcgcg cggcttctgc caataccgca
      601 ggttgcaact ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc
      661 gcatcctgcg cgttactggc atacatcagc gcgcaactat cccattccag ccatgcattt
      721 ttgtcctcac ctaaataggt gctaaacgct ttgattcccc acgggacgct gcacggattc
      781 acaatgggcg caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc
      841 agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat
      901 tgcgactgaa ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc
      961 gcccacggcg gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca
     1021 tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc
     1081 agttccgccg ctacccgctg ggcaccagcc ttggtggtga agttctcgtc attgcaggtt
     1141 aatccggaaa gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag
     1201 atactgaacg tcatcgggca gtttaaggta ctggagtcgt gtctccagcg ttgctgccag
     1261 ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg
     1321 caatttcttc atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg
     1381 cataacgatg ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg
     1441 tgaatactgg attatgtgcg ccgcctcacg cacaataatc aagctgtaaa tcagcttaat
     1501 aactttgccc ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc
     1561 actcagtaaa gaagcggccc tggttcatga agcgttagtt gcgagaggac tggaaacacc
     1621 gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca
     1681 tatgaccgaa atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac
     1741 gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa
     1801 tttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg
     1861 cgatatcact ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac
     1921 ggtggcttat atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca
     1981 gttctttgcc cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct
     2041 acaaacgctg ctgggcacca ataacgtggc tgtctcgatc gacgcggtgc attactgcgt
     2101 gaaggcgcgt ggcatccgcg atgcaaccag tgccacgaca acgacctctc ttggtggatt
     2161 gttcaaatcc agtcagaata cgcgccacga gtttctgcgc gctgtgcgcc atcacaactg
     2221 attaaaaggc aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca
     2281 ttctggggat cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca
     2341 atcccgcctg gtacgatgct attacgccgc aggatgcatg gacctgggca tttctcgatc
     2401 tcatcgggca ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa
     2461 tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgttgg
     2521 gctttattca cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg
     2581 gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg
     2641 gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca
     2701 gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga
     2761 agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac
     2821 tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc
     2881 tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt
     2941 ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc
     3001 tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc
     3061 cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct
     3121 ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc
     3181 aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat tttgaccgcc
     3241 tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct
     3301 ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg
     3361 ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa
     3421 aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg
     3481 acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga
     3541 tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc
     3601 tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg
     3661 agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca
     3721 ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg
     3781 atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg
     3841 aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc
     3901 actcaaaagc attgagtgac gatgaactgg cacaatttat ggataacatt cccggtatgg
     3961 tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca
     4021 gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc
     4081 tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt
     4141 tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc
     4201 cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctgtat
     4261 ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg
     4321 cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg
     4381 acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac
     4441 tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga
     4501 cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa
     4561 caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ctaacaatgt
     4621 gatagctatg attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt
     4681 tcaccgaggt aatagcgtca taacaataat taaagccgtt ttctggagcg ttaccgggca
     4741 tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact
     4801 gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga
     4861 ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac
     4921 acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg
     4981 tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga
     5041 atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag
     5101 gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga
     5161 aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc
     5221 tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc
     5281 agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg
     5341 gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta
     5401 ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag
     5461 ataccgcaat gtaggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc
     5521 cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg
     5581 tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc
     5641 cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta
     5701 acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg
     5761 ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag
     5821 ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact
     5881 gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt
     5941 ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac
     6001 ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg
     6061 gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca
     6121 ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg
     6181 gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca
     6241 tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg
     6301 aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca
     6361 cattatccgt ttcgcaaatg cagatgattg aaatcgccaa agcgttttcc tataacgcga
     6421 aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt
     6481 tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa
     6541 tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg
     6601 ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt
     6661 ctcttaatca gcgtttccct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc
     6721 gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag
     6781 gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt
     6841 tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata
     6901 accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct
     6961 caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca
     7021 actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg
     7081 tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt tcgctctccg
     7141 gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa
     7201 tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa
     7261 ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt
     7321 tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg
     7381 atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt
     7441 aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg
     7501 gtatttatgt cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt
     7561 taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc
     7621 tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt cgtcaggtag
     7681 ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc aacaaagtgt
     7741 tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg
     7801 cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg ccgttcatta
     7861 ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg
     7921 gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg
     7981 cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg
     8041 tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc
     8101 cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt
     8161 tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca
     8221 ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg
     8281 tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta
     8341 ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag tacatcatca
     8401 aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaagt
     8461 gatgtgattg cggatattgc cggatgcgct gcatccggca atcggtgcca aatgccggat
     8521 gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg cctttcttaa acttcacttc
     8581 cccgagctca atacaaccca ccgggcagac gtgaccacaa agcagacaac ccacacattt
     8641 ctcggtatta caatgcggtg tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc
     8701 gtcgtaacag gaaatataac agcgtccaca gccaacacat ttgtcaagat tgatacgggg
     8761 atagacaata taactgcggt ctaaatcttc cgccgggaca atattgttat tcgccagacc
     8821 taccatctcc tgcaacgaat caaatccctg atcggcgaga taatgcgata acccgctcgc
     8881 catatcttct acgatgcgat agccgtactg cataatgccg gtggtgacct gtaacgttgc
     8941 tgcgccgagc aataaaaact ccgccgcatc ttcccaggtt tcaatgccgc cgataccgct
     9001 gattgggaaa tcgcgcagtt ctggatgggt gcgcatttgc tgaatgaagc gcagggcgat
     9061 cggttttacc gctttaccgg aatatccgga aatactcgat ttaccgttaa cgatcggcat
     9121 accgattttc tgattaagat cgatattggt gatggattta acggtgttaa ttgccgcaat
     9181 gccatctgcg ccgccgcgct tcgccgccag cgccacttcg cacatatcac cgatattcgg
     9241 cgtcatcttc gccagcattg gcagcgtgga accccgtttc actgcccgac aatatttttc
     9301 taccagctcc gggctttgcc cgacatcgct acccatcgca tgagaagtca tttgcggaca
     9361 ggagaagtta cactcgatca tatccgcgcc agcttcttgc accaggcgcg ccagctcctc
     9421 ccattgctgc tcattttccc ccatgatcga agcgatcaat actttgtccg ggtaatcttc
     9481 cttcagccga cgcagggcgg tcagattctc ttccaacgga tgttcagcaa tctgctccat
     9541 atttttgaag ccgataaaac cggtatcttc tttcaccaga tgatcaaaac gcggcgagac
     9601 ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta
     9661 ggctttggca cacatctcat agcagttgcc taccggcgaa gaagagaggc agaacgggtt
     9721 gggaaagttc acgccgcaaa aagtaatcga aagatctttc gttaacatga gcaagctccc
     9781 tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc atagaccacg
     9841 gttttgtcac cctcaacaat atcgccagca gcaaagactt gcggatcgcg ggtctgataa
     9901 ttttgtgttt tgatggtgtt acgctgctgc tctaactccg caaaggcatc cagtctggca
     9961 tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc tgataaccgt
    10021 acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc atcgatgatc
    10081 gaaacgccca gctcccgggc gctggcaaac tctttttcgc ttgccggaaa ctcatctaac
    10141 tcttcacgtg ctacgcaagt taccgcctga cagccgagaa ctttcagcgt gctggctacg
    10201 tccatcgcga catcaccgcc gccgataatt aatgcgcttt gtggaatgct gatatcgcct
    10261 tgcgcctgtc gtacacgttg caggaagtcg acggcaatct caacgtcgct atgctcaaac
    10321 agcggtagcc cggaaccgct tgataacccc acggtgacca gtaccgcgcg gttttccgct
    10381 ttcagttgct ccagggtgag tgttttaccg atttcgttat tgcacttaat ggtcacgccc
    10441 attttttcaa tacgggcgat ctctgcatcc agcactgatt gcggtaaccg gaattgcgga
    10501 ataccgttac gcaaccagcc accggggtgc gcttctttct cataaatcgt gacgtcataa
    10561 ccctggtttg tcagtgtcac actggcctgc aatccggcag gacctgcgcc aataatcgcg
    10621 actttgccga gcgttttagt accgggctga taaatttcca tcccggtttg ttgttcaaaa
    10681 tcagtaacaa aacgctgtaa gcggccgata tcaatgggag catcaacacc ggcacgggta
    10741 caaccgcttt ggcataattt ctccgtcggg cacactctgg cacaaacggc accgagggca
    10801 ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat tgagcgaata
    10861 aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc gtgacataat
    10921 aaacagcgag aggcttcttt aatcgccagt aacggcgtaa aagccggagt gagttcatcc
    10981 agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattat ggcgaacgct
    11041 aaataaatat tgcgtgctga tattaaggct atgcgcgtgg ggaaatttaa actgtgaaag
    11101 gattcacaaa tagctgcggt tcagaatgtt atatccgcag cttactgagt gaagtaataa
    11161 ttattctcaa taattaacag aaaagagaaa atattaacga tagaaaataa gataaacaat
    11221 catgccgata gataaaattc cgaagccaat atatgccacc atataagcga agaagagtcc
    11281 gggatttttt gctcgccaga catacaccgg ttccggtgca tttttaaccc gtttatcaat
    11341 cgcgcctgcg cgccagcctt tccatgcttc gcggaaacaa aatatcggga taaggaaaaa
    11401 aacggcaacg acaaaaaact gctgtacatc cataactact ttccttgttt cttttgtaat
    11461 tcaagtaact gttcgggtgt aacggcggga tacccctgcg catcttccgg cacctggtgc
    11521 atagccggaa tagggaccag cggccctaaa aaacgcggtt cacgtttaaa aatataaagg
    11581 tcagccagcg caccgaaacg ggcggcaaat tcgcgaatac gtactgacag catatctttc
    11641 ggtgacggta cggcataaca ctgagcctga atccccatat gcagcgcaat aaataatgct
    11701 cgctcacagt ggaaacgttg ggtgataata atgaaatcat tagtatcgaa aactttgcgt
    11761 gtacgcacga tggaatccag cgtacgaaag cctgcgtaat cgagaacaat atctgatggg
    11821 tcgacaccag cagcgattaa atctttgcgc atggtcatcg gctcgttata actttgcaat
    11881 gcgttatcgc cgctcagtaa tagataattt accttaccgc tgttataggc attaatcgct
    11941 ccttgaatgc ggtagcgata atactgatta ataacgccgg tacgataata ttttgctgtg
    12001 ccgagcacca cgccgacctg gcggtagggg agatcctgca attcgtcgta gatataaggc
    12061 gcggttttcc agctcatcca gcgatcgagg ccgagcacag tcaacagcag caagccgatc
    12121 aggactaaca ggctgaggaa cacgcgcttt aacatgcagg agactcgagc taaggaaaat
    12181 ttccatcagg ctactttacc cgctggtttt gcgcaagaaa cggcaaactg tttgccggga
    12241 atttcgacac caggagaggg ctggcaagcc ggacaaggcg ttcacgccgc atccggcacc
    12301 aggcttaagc gagaagcacg cggtcgatac tgtgacagcc gagagctttc agcgttgcgg
    12361 aggtggcatc ccactgaatc aacggcgcat cggctttttc tgccagaacc gcgcgctgga
    12421 tatccgggta atcataaccc ttgagattca acagaattaa cgccccctgc aacggcggca
    12481 gagtcgggtt gaatgcggcg ttttcagcgt agctgccgct gaaaatacga ccgtctttac
    12541 attccagcgc gacaccgctt ggcgacttac tgtaaggcat gtgcgaacgg tttgccgctg
    12601 caatcgccgc ctgagaaagc gcatcaccag tcagcgcata gccgtgatcc tgctcgtcca
    12661 tcagcagcgt tttaatctcc agatctttcg gcccaaaggc atccggcaga tagtcacgca
    12721 gcgcgtgtgc ctcgcggccc ggcaaatgaa tacgcagatc cagaccgctg ttcagttcat
    12781 tcataaactg acggcagtga ccacaaggcg tatagttaac ggtgatggct gcaagcgctt
    12841 tttcaccact caaccaggcg tggctgatcg cgctttgttc ggcatgaacg gtttgctgca
    12901 ttgttgcacc aataaattcc atattggcac caaaatacca ggttccgctc acaccgcgcg
    12961 caattgcgcc aacattaaaa ttcgacaatg gcgtacgcgc acaggccgcc gccagcggaa
    13021 gtagtgcgaa tgccagcgcg tcttcgtcca gccccgttgc gctcttcagc gatgagactt
    13081 gctccccggt caacaaagcg gggaagtact tgtctgccag aataggttcc agtgcagatt
    13141 gcaagttatc cgcaagttgg gcaaaagcgg tttgaaaacg tggatgcatg gcgttgcctc
    13201 ataatcatgt aatgggataa cagtttacga acccgttatg gctttatatg tgatctgaat
    13261 ctcattaatt atgcaacacg tgaaacttac atcaaaaatg cgcgacgtat cgcaaatttt
    13321 agcccattac tgccagaata atcgggaaaa ggaacggtgc gatcagcgaa gtaattatcc
    13381 cgcataacac cagcgctagc gaactaaatg caccttcctg ataatccagc tcggcgcagc
    13441 gcgccgtacc gagggcgtgc gaggcagtcc ccattgccag accgcgcgca gctttggtac
    13501 gaatacgcat cgcattaagc aatgtatggc caaataccgc gccgaggatg ccgacgaaaa
    13561 tcacgcaaac tgcgctgatt gccggaatac cgccaatact gccgccaacc gccattgcaa
    13621 taggcgtggt gactgatttc ggcaggattg acgcggcgat ttccggtgaa gcgcccatca
    13681 acaatgccac ggaagtcccc gtcaccatcg caaccacgct gccgataaaa cagatggtga
    13741 tgatcgattt ccagcgcgcg cggatctggt gtagctgctc atataaagga taggccagcg
    13801 cgacgaccgc cggttgcagc agatcgttta gcacttcgct acctttaaag taactgtcgt
    13861 aagagatgcc ggtcagcatt aaaaaaggaa tgatcaccac cattgctacc agcaacgggt
    13921 taagcagggg aaatttatac cgtgccgcca gtttgcgggc ggcaaaaaag acaatcaacg
    13981 tcagcggtaa tgaccaccag atattcgcca tcattcttct gatcctttct gacctacgac
    14041 tttacgttca ccgtgtacca gttgtgaact ccagctcacc accagaaaaa ccaccagcgt
    14101 actgacagca caggaaacca ctaccgggcc aaactgcgcg cggagcaaat caaaatattg
    14161 catgacgcct acgccaatcg gcacaaacaa tagcgccata tagcgaatca gtatgtagca
    14221 ccccggattg acccatttcg ccggaagaat ttgcaaggcc agcaggacaa acaggatcag
    14281 catcccgatg atgctgcccg gaatggttac cggtagcagg gaagcaatga aaatgcctgc
    14341 atacaggcag gcataaatca ggacgaaagc gcgtaaatat tgccagataa tgttcagtgt
    14401 cttactcata atcaacaccc cagatgagat ggattcatca tacaattaat tcgtaaaacg
    14461 tgctaccgat cacattaaga aatcagcgct tattttttcg ctacccggta ttacgcaatg
    14521 agcagcgaac tcaggcatac atcaagcata gagagggatt gcagtttgca gcaatccctc
    14581 tttgcagacc tttaatccgc tgaccatagg cgctaacata gcgcctcatt tttttgcggg
    14641 tgatgatatg cgtgtgttac tggcaccgat ggagggagtg cttgactctc tggtgcgtga
    14701 attgctgacc gaagttaacg actacgatct gtgcatcacc gagtttgtcc gcgtggtgga
    14761 tcaactgctg ccggtaaaag tctttcatcg catttgccct gagctacaaa acgccagccg
    14821 gacaccatct ggtacgctgg tgcgcgtgca gttgttaggt cagttcccac aatggctggc
    14881 agagaacgcc gcccgtgcgg tggagttagg ttcctggggc gtggatctca attgcggctg
    14941 cccgtcgaaa acggttaacg gtagcggcgg cggggcgacg ttactcaaag atcctgaact
    15001 catctactag ggggcaaaag cgatgcgtga agctgtaccg gcgcatttgc ctgtcagcgt
    15061 gaaagtgcgt ctgggctggg acagcagtga gaagaaattt gaaatcgccg atgcggttca
    15121 acaggctggc gctacggagc tggtggtgca tgggcggacg aaagagcagg gttaccgcgc
    15181 ggagcatatt gactggcagg cgattggcga gattcgccag cggctgaata ttccggtgat
    15241 tgccaacggt gaaatctggg actggcagag cgcgcaacaa tgcatggcga tcagcggctg
    15301 cgacgcagtg atgattggtc gcggggcgct caatattccc aacctgagcc gggtggtaaa
    15361 atataacgaa ccgcgaatgc cgtggccgga ggtggttgct ttgctgcaaa aatatacccg
    15421 tctggaaaag cagggcgata ccgggttata tcacgttgcg cggattaaac agtggttgag
    15481 ttatttgcgt aaagaatacg atgaagcaac ggaattattt cagcatgttc gggtgttgaa
    15541 taactcccct gatattgcaa gggctattca ggaaattgat atcgagaaac tctaaaaacc
    15601 atagtgatgt ttttgacaat gtgttagctc tttatcaata ggccgcacgg ttgtttggtg
    15661 gcgtcaaaat cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga
    15721 gggcgcgttt tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagccg
    15781 agggcaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcaggg ggcggcggcg
    15841 attggtcgcc ccctgcgcgc tccttgcgcc agtgattata tgaagcaatg cacatgacag
    15901 gagcgcaaca ggatgagaga ctgaaatatt ctcgttattt tcggatctga tgacattaat
    15961 taattttaaa atatcatctt aaatacgcca gtcaccacca gtagcccaat caggaaaata
    16021 atcaatacag cccagagtat aattttcatt ggcgttcctt tatttgtgag tgctgaataa
    16081 gtataggaaa aaacgaagcc ctttgccgtt atccataaac gattaagata aatcggaaga
    16141 tatcatttac ttagtctata tttttctggg tatgtcgttt tattgctact gatgtattgc
    16201 cacgcgaaac taaaaaatat atcgcaacac acggtaatct ccgcgcaccg tttcttacct
    16261 gattattccc ccatgaatcg tgattccttt tatccagcca tcgcctgttt tccgctgtta
    16321 ctgatgctgg ccgggtgtgc gcctatgcat gaaacccgcc aggcgttaag ccagcaaacg
    16381 cccgctgcac aagttgacac cgcattaccc acggcgctga aaaatggttg gccagacagc
    16441 caatggtggc tggagtatca cgataatcag ctcacttcat taattaacaa tgcactacag
    16501 aacgcaccgg atatgcaggt ggctgaacag cgcattcagc tagctgaagc ccaggcgaag
    16561 gcagttgcca ctcaggatgg tccgcagatc gacttttcgg cggatatgga gcggcaaaaa
    16621 atgtcggcag aagggctaat ggggccgttt gctctgaacg atccggccgc aggtacgacc
    16681 ggtccgtggt acaccaacgg aacttttggc ttaacggcgg gctggcatct cgatatctgg
    16741 ggaaagaatc gggcggaggt tactgcccgc ctgggtacgg ttaaagcacg ggcggcggaa
    16801 cgcgagcaaa cccgccaatt gctggctggc agcgtagccc gcctgtactg ggagtggcaa
    16861 acccaggcgg cgttaaacac ggtcttgcag caaatagaaa aagagcagaa caccattatc
    16921 gcgaccgatc gccagctata tcagaacggg attacttctt cagttgacgg tgtggaaacc
    16981 gatattaatg ccagcaaaac ccggcagcag ctcaacgatg tcgcggggaa aatgaaaatt
    17041 attgaggcac ggttaaatgc actcacaaat caccagacta agtcattgaa gcttaaaccg
    17101 gtcgcgttgc cgaaagtggc aagccagctt cctgatgaac tggggtactc cttactggcc
    17161 cggcgggcag atttgcaggc ggcgcactgg tacgttgagt catcgttaag cactattgat
    17221 gcggcaaaag cggcatttta tcctgacatc aacctgatgg ccttcctgca acaggatgcg
    17281 ttgcacttaa gcgatctgtt ccgtcattcc gcgcagcaaa tgggcgttac ggcaggcctg
    17341 acgctaccta ttttcgatag tggtcgtctt aacgccaatc tcgatatcgc aaaagccgaa
    17401 agcaacttgt ctatcgccag ctacaacaaa gcggtggttg aagcagtgaa tgacgtggcg
    17461 cgggcagcca gtcaggttca gacactggcg gagaaaaacc agcatcaggc gcaaattgag
    17521 cgcgatgcct tgcgtgtggt aggtcttgcg caggcccgct ttaacgcggg catcattgct
    17581 ggttcccgcg tcagcgaagc cagaatcccg gcgctgcgtg agcgggccaa tggcctgtta
    17641 ttgcaagggc agtggctgga tgcctccatt caactcaccg gtgcgctggg cgggggttac
    17701 aaacgctgat gatatgcgcc ttctatactt aacgtttatt cagcgttaag tggagaactc
    17761 gatggcatag gtcgcgataa ttaccgcttc cgattcgggg atcggcaaag agtgcgcgtt
    17821 attactggcg cagcaggggt ttgatattgg cattacctgg cactcagatg aagaaggggc
    17881 aaaagatacc gcgcgtgagg tggttaccca cggcgtacgt gcggagatcg tgcaactgga
    17941 tctcggcaat ctgccagaag gtgcgcaggc gctggagaca ctcatccagc gatttgggcg
    18001 tattgatgtg ctggtgaata atgcgggtgc aatgaccaaa gcgccgtttc ttgatatggc
    18061 ttttgatgag tggcgcaaga tttttaccgt tgatgttgat ggtgcatttt tatgctcgca
    18121 aattgcggct cgtcagatgg tgaaataagg gcagggcggt cgcatcatca acattacgtc
    18181 ggtacatgaa catacgccgc tgccggatgc cagcgcctac acagccgcta aacatgcgct
    18241 cggcgggtta accaaaacga tggcgctgga gttggtcagg cataagattt tggtgaacgc
    18301 agtcgcgtct ggggcgatag ccacgccaat gaacggcatg gatgatagcg acgtgaagcc
    18361 cgacgcggag ccttcgattc ctctacggcg ttttggcgca acgcatgaga ttgccagctt
    18421 ggtggtgtgg ctttgttcgg agggcgcaaa ttacacgaca gggcagtcgt tgatagtgga
    18481 tggcggcttt atgttggcga atccgcagtt caacccagaa tagcgcagtg aggtgcgtgg
    18541 atatttcaat gggttaggcc agataagaca ccagcgtcgc atcaggcatg gaacacccgg
    18601 tgccggatgt ggagtgaatg cttatctgtg gcaactgctt tacgcctgat tatccggctt
    18661 tttcttcccg cgccgtttca gccaccagcg cacgcccacc accagaacca cgaccagaat
    18721 caaccagacc cagtgcttca aatgctggtc gagattgtgc aaccacggcg caatcacctg
    18781 accaccagcg taaccaatag tggtaaaaat caacgcccag gcaaatgcgc cgagaatatt
    18841 cagcggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg ggccaatcac
    18901 ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc gttggataag
    18961 tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca gcttgccgcc
    19021 aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccaccaa gcgccacaga
    19081 aagtaccacc agtgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt
    19141 cacggtttca ccttccgcca ggctaccgat aaccagcgcg gcataaccat attgtgagat
    19201 gagtgtattg agatccatag gccagtctgt ctccctttgg ttgcctgtaa gcatacaccc
    19261 tgtgaatcat tcacgctgaa aaaggacata catcagacgg tgcaaattac atggatgaac
    19321 aaaaaataat cacaagctgg cttatacttg aactgtcagg ttcatgaact cacaacaagg
    19381 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa tgcaggtgat
    19441 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
    19501 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
    19561 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
    19621 tatgctggct ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gcaattaccc
    19681 gcagcgtccg tctcttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
    19741 tttaagcggt ctggtggcgc tttatccact ggtctggttg tgtgcgctgg tcggcacggt
    19801 ggcgctgttt tacaccggct acctgcttta ccttggtatt ccgagcttcc tgaatatcaa
    19861 caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc tggtacagga
    19921 agtgctgctc gccctaaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt
    19981 tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttactc acgattctcc
    20041 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttact
    20101 ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat gccgaaattt
    20161 cgagtttctt tatttagcct ggccctgatg ctggctgtgc cttttgcacc gcaggccgtt
    20221 gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg tagcgcgatg
    20281 attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct ggtgcgtccg
    20341 attgcgtcta tcagcaaatt aatgaccgcg atggttgtgc tggatgcacg actgccgctg
    20401 gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt ctattcgcgc
    20461 gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct gatgtcttca
    20521 gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa agcctttatt
    20581 aaggcaatga atgcgaaagc gaaatcgctc ggaatgaaca acacgcgctt tgttgaacct
    20641 accggattgt cggtgcataa cgtttcaact gcccgtgact taaccaaact gctcattgcc
    20701 agcaaacaat atccgttgat cgggcagtta agtactaccc gggaagatat ggcaaccttc
    20761 tctaacccga cgtatacgct gccgttccgc aatactaatc atctggtgta tcgcgataac
    20821 tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg tctggtgatg
    20881 cgtacggtta tcaataataa accggtggcg ctggtagtga tggacgcgtt tggcaaatat
    20941 acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa agtgatgcct
    21001 gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc ggcggcgggg
    21061 cagacggcac agaacgatta agtgaattcg gatggcgata ctctgccatc cgtaattttt
    21121 actccacttc ctgccagttt ttccgtttac tggttttacc gatccccgga ttcatgctat
    21181 tggtcggatc gttctcgcga tagaacttct gcaacgtctc cggtgcttta tacaaatgac
    21241 cgacgttatg ctcggcaggg tactgcgcgc cgcgctgctg tagcagttcc agcatctgtt
    21301 cttttaacgc atgcacatcc acgccttttt tcactatgta atcctgatgg aagacatagc
    21361 acataaaatg gccgtaatag agcttgtgca ccagctggct gtcgatctcc ggcggtaaat
    21421 gctcatacca ctcggtgtcg ttacgccgca gagcgatatc cagtgccaga atgtcttcga
    21481 cttcatcgga atgcaccgcc tgataacgaa ttgctgcgcc cgcagcggcg aaacggtgta
    21541 aaaacgcttt gctaccttcc tccggcgtac agacaaagaa atcgccctcg gcctgtttga
    21601 aataatccac cagccacgat ttggcttcac cgacgccatc gcctgccatt tttaacagca
    21661 gatgatgctc gtatttatcg cgccagtttt tcatgcgcgg cggtaaatgg ctggggaaca
    21721 ggtgaccgaa tttttgcatt gcacggtcag taaaatgcgg acggaagaat ttcactttct
    21781 ccagcatcgc atcggtgcgt cccttgagat taaagaagaa cggcatcttg tcggtgccta
    21841 acttatcaat catcaggaag gtgtctttgc cgtatttttc cgcaatatcg tagatatccc
    21901 ggtgcatata ttccccggca accggcagat tttcgaagtt agccagaata tgacggcgga
    21961 tttcggtcag cacttccggc tggttggtgc cgatataaaa cacctgctga tttttttccg
    22021 cttcgaaggt atcaagacgt accgcaaaga cggccagctt cccggcgcaa ccagaagatt
    22081 caaataaccg atccggatcg gcgttataac gtgcgggcgt gtcggcttca atatcacgaa
    22141 cgcggtggac atagtcataa tcgtgggcgt gacgaccatc gtgacgcaca tcgtcatctt
    22201 tgatgcgatc gtcatccagc ttgctaagga tctgctccgg cgtttcgccc agatcaatcc
    22261 ccagatggtt caccagcgtc agtttgccat cttcatctat acgcgcgaat aacgacattt
    22321 cggtatacgc cgggccacgt tgcaccagcg agccgccgga gttgttacaa ataccgccga
    22381 tgaccgatgc gcctatacac gatgatccaa tcaccgaatg cggttcgcgt cccagcggtt
    22441 tgagggcttt ttccagcgaa tagagcgtgg tgcccggata ggccagcacc tgttcgccct
    22501 tgccgagaac gtgcagcttg tcgagacgca gggtgctgat gatcacgata tcgcgatcat
    22561 agtcgttacc gtttggcgtc gatccttcgg tcaggcctgt attggcagcc tgcatcagaa
    22621 taattttgtc ggcggtgacg caggctttca gcacccgcca caattccagt agtgagccag
    22681 ggaaaacgac agccagcgcg tcgccctgac cagaacggaa gcccttgcga tagcgggccg
    22741 tttttgcggg atcggtgagc aggtgtgaat gaccgaccag acgggcaagt tcattcaaaa
    22801 aggctttatt atcagttgtt gtcatggaag acattttcca ctccttgtgg tggcgaaaaa
    22861 atatcacaaa ctagaatagc gccttgtatg acaattcagc gagtgaatat tttgaaaatt
    22921 cagacaataa actgagcatt ctttttctct ctccatcatg cttatggcaa actacggttt
    22981 ttgcaccatt ttcgctgcca cgtcgggcaa caaaggaaga aaaatccata tgaaatggct
    23041 atgttcagta ggaatcgcgg tgagtctggt cctgcagcca gcactggcgg atgatttatt
    23101 cggtaaccat ccattaacgc ctgaagcgcg ggatgcgttc gtcaccgatc tgctcaagaa
    23161 aatgacggtt gatgagaaaa ttggtcagct gcgcttaatc agcgtaggcc cggataatcc
    23221 gaaagaggcg atccgcgaga tgatcaaaga cggtcaggtt ggggcgattt tcaacaccgt
    23281 aacccgtcag gatatccgcg ccatgcagga tcaggtgatg gaattaagcc gcctgaaaat
    23341 tcctcttttc tttgcttacg acgtgctgca cggtcagcgc acggtgttcc cgattagcct
    23401 cggtctggcc tcgtctttta acctcgatgc ggtgaaaacg gtcgggcgtg tctctgctta
    23461 tgaagcggca gatgatggcc tgaatatgac ctgggcaccg atggtcgatg tctcgcgcga
    23521 tccgcgctgg ggacgtgctt ccgaaggttt tggcgaagat acgtatctca cctcaataat
    23581 gggtaaaacc atggtggaag cgatgcaggg taaaagcccg gcagatcgtt actcggtgat
    23641 gaccagcgtc aaacactttg ccgcatacgg cgcggtagaa ggcggtaaag agtacaacac
    23701 cgtcgatatg agtccgcagc gcctgtttaa tgattatatg ccgccgtaca aagcggggct
    23761 ggacgcaggc agcggcgcgg tgatggtggc gctgaactcg ctgaacggca cgccagccac
    23821 ctccgattcc tggctgctga aagatgttct gcgcgaccag tggggcttta aaggcatcac
    23881 cgtttccgat cacggtgcaa tcaaagagct gattaaacat ggcacggcgg cagacccgga
    23941 agatgcggtg cgcgtggcgc tgaaatccgg aatcaacatg agtatgagcg acgagtacta
    24001 ctcgaagtat ctgcctgggt tgattaaatc cggcaaagtg accatggcag agctggacga
    24061 tgctgcccgc catgtactga acgttaaata tgatatgggg ttgtttaacg acccatacag
    24121 ccatttgggg ccgaaagagt ctgacccggt ggataccaat gccgaaagcc gcctgcaccg
    24181 taaagaagcg cgtgaagtgg cgcgcgaaag cctggtgttg ctgaaaaacc gtctcgaaac
    24241 gttaccgctg aaaaaatcgg ccaccattgc ggtggttggg ccactggcgg acagtaaacg
    24301 tgacgtgatg ggcagctggt ccgcagccgg tgttgccgat caatccgtga ccgtactgac
    24361 cgggattaaa aattccgtcg gtgaaaacgg taaagtgctg tatgccaaag gggcgaacgt
    24421 taccagtgac aaaggcatta tcgatttcct gaatcagtat gaagaagcgg tcaaagtcga
    24481 tccgcgttcg ccgcaagaga tgattgatga agcggtgcag acggcgaaac aatctgatgt
    24541 ggtggtggct gtagtcggtg aagcacaggg gatggcgcac gaagcctcca gccggaccga
    24601 tatcactatt ccgcaaagcc aacgtgactt gattgcggcg ctgaaagcca ccggtaaacc
    24661 gctggtgctg gtgctgatga acgggcgtcc gctggcgctg gtgaaagaag atcagcaggc
    24721 tgatgcgatt ctggaaactt ggtttgcggg gactgaaggc ggtaatgcaa ttgccgatgt
    24781 attgtttggc gattacaacc cgtccggcaa gctgccaatg tccttcccgc gttctgtcgg
    24841 gcagatcccg gtgtactaca gccatctgaa caccggtcgt ccgtataatg ccgacaagcc
    24901 gaacaaatac acttcgcgtt attttgatga agctaacggg gcgctttatc cgttcggcta
    24961 tggtctgagc tataccactt tcaccgtctc tgatgtgaaa ctttctgcgc cgaccatgaa
    25021 gcgtgacggc aaagtgacgg ccagcgtgca ggtgacgaac accggtaagc gcgagggggc
    25081 cacggtagtg cagatgtact tgcaggatgt gacggcttcc atgagtcgcc ctgtgaaaca
    25141 gctgaaaggc tttgagaaaa tcaccctgaa accgggcgaa actcagactg tcagcttccc
    25201 gatcgatatt gaggcgctga agttctggaa tcaacagatg aaatatgacg ccgagcctgg
    25261 caagttcaat gtatttatcg gcactgattc cgcacgcgtt aagaaaggcg agtttgagtt
    25321 gctgtaatgc tttagtaagg gcgcgacgtt tgcgcccttt gcttgatgtt catatcttaa
    25381 cttgctgaat tttcgccctt tgtaggccgg ataaggcgct cgcgccgcat ccggcaggaa
    25441 acgccggacc gaatatgcct cttaagtatt tatctgattc tgcaactgaa tccttccgct
    25501 caggctaact ctgtcattat caactatgct tttctcttaa ttcgctgaaa acaacagtaa
    25561 aagaatgagg aaagcatcat gccactctta aagctctggg caggttcact ggttatgttg
    25621 gcagccgtga gcctgccgct acaggcggct tcccccgtta aagtcggttc aaaaatcgat
    25681 accgaaggcg cgctgctcgg caacatcatc ttgcaggtac tcgaaagcca cggcgtaccg
    25741 acggtgaata aagtgcaact cggaacgact cctgtggtgc gcggggcgat tacttccggt
    25801 gaactggata tctatccgga atataccggc aatggcgctt tcttctttaa agatgaaaac
    25861 gatgcagcgt ggaaaaatgc cgggcagggc tacgagaaag tcaaaaagct cgatgcggag
    25921 cacaacaagt taatctggct gacgcccgcg cctgcaaata acacctggac tatcgccgtg
    25981 cgtcaggatg tggcagagaa aaacaaactc acttcgcttg ccgacctgag tcgttatctg
    26041 caagagggcg gcaccttcaa actggcagcc tcggcagagt ttatcgaacg cgccgatgcg
    26101 ttacccgcgt ttgaaaaagc ctatggcttt aagctcggtc aggatcagtt gctgtcactg
    26161 gctggtggcg acacggcggt gacgattaaa gccgctgccc agcaaacctc tggcgttaat
    26221 gccgcaatgg cttacggcac tgacggtccg gtcgcggcgc tggggctgca aaccttaagc
    26281 gatccgcaag gcgtgcaacc tatctacgcg cctgcaccag tggtgcgtga atcggtgttg
    26341 aaggagtatc cgcaaatggc acagtggcta cagccagtct tcgccagcct cgatgcaaaa
    26401 acattgcagc aactgaatgc cagcattgca gtggaaggac tggatgccaa aaaagtggct
    26461 gccgactacc tgaaacaaaa agggtgggcg aagtaatttc ccgtgactta tctacgtatt
    26521 aatcctgttc tggcgctgct gttgttgctg acggcaatcg cagcggcgct gccgtttatc
    26581 agttacgcgc ctaatcgttt agtctcgggt gaagggcgtc atctctggca actgtggccg
    26641 caaacgatct ggatgctggt gggcgttggt tgcgcctggc tgacagcctg ttttgtcccc
    26701 gctaaaaaag gcagcatttt tgcactcatt ctggcgcaat tcgtcttcgt attgttggtg
    26761 tggggagctg gaaaggcggc gacccaactg gcgcaaaatg gcagtgcgct ggcgcgtacc
    26821 agcctcggca gtggtttctg gctggctgcg gcgctggcat tgctggcctg tagcgatgcc
    26881 atccgccgaa tctccacgca tccgctgtgg cgctggttgt tgcatatgca gattgccatt
    26941 attccgctgt ggttgctgta ctccggcacg cttaacgatc tctcactaat gaaagaatac
    27001 gccaaccgtc aggatgtgtt tgacgacgcg ctggcacaac atctgacgtt gctgtttggt
    27061 gcggtgctgc ctgcgttagt gattggtgtg ccgttgggca tctggtgcta cttttccact
    27121 gcgcggcagg gggcaatttt ttctctgctc aatgtcattc agaccgtgcc ttcggtggcg
    27181 ctctttggcc tgttgattgc gccgcttgcc gcgctggtta cggcctttcc gtggctgggg
    27241 aagctcggca tagcaggaac cggaatgaca cccgcactga ttgcgctggt gctctatgcc
    27301 ttgctgccgc tggtgcgcgg cgtggtagtc ggtttgaacc agatcccgcg cgatgtgctg
    27361 gagagcgcca gagcgatggg gatgagcggg gcgcagcgtt tcctgcatgt tcagttaccg
    27421 ctggcgttac cggtatttct gcgcagcctg cgggtggtga tggtgcaaac tgtaggtatg
    27481 gcggtgattg cggcgttaat cggcgcaggc ggttttggtg cgctggtttt ccaggggctg
    27541 ctaagcagcg ccattgattt agtgttgctg ggggtgatcc cggtaattgt tctggcggtg
    27601 cttaccgacg cgctgttcga tttgcttatc gcactgctga aggtgaaacg taatgattga
    27661 atttagccat gtcagcaaac tgttcggcgc acaaaaagcc gttaacgatc tcaatctcaa
    27721 ttttcaggaa gggagttttt cggtgctgat tggcacatct ggctccggca aatccaccac
    27781 cctgaaaatg attaaccgcc tggtggagca tgacagcgga gtgatccgct ttgccggaga
    27841 agaaattcgc tcgctgccag tactggagtt gcgccgccgg atgggctatg ccattcaatc
    27901 tattggcctg ttcccacact ggagcgtggc gcaaaacatc gctaccgtgc cgcaattaca
    27961 aaaatggtcg cgggcgcgga ttgacgatcg tatcgacgaa ttaatggcgc tactggggct
    28021 ggagtcaaat ttgcgtgagc gttatccgca tcagctttcc ggtggtcagc agcaacgtgt
    28081 gggagtggcg cgtgcactgg ctgccgatcc gcaagtctta ctgatggatg aaccttttgg
    28141 cgcactggac ccggtaacgc gcggcgcgtt gcaacaagag atgacgcgca ttcaccgttt
    28201 gctggggcgc actattgtgc tggtcactca tgatattgat gaggcgctac ggctggcaga
    28261 acatctggta ttgatggatc acggtgaagt ggtgcagcag gggaatccgc tgacgatgct
    28321 gactcgtccg gcgaatgatt ttgtccgcca gttttttgga cgtagtgaac tgggggtgcg
    28381 cctgctttcg ttacgtagcg tggcggatta cgtgcgtcgc gaagaacggg cagaaggtga
    28441 ggcactggca gaagagatga cgctacgcga tgcgctctcc ctgtttgtcg cgcggggatg
    28501 cgaggtgctg ccggtggtga acacgcaggg ccagccttgc ggcacgctgc attttcagga
    28561 tctgctggtg gaggcgtaag cgtatgaaga tgttgcgcga tccgctgttc tggctcattg
    28621 ctctttttgt ggcgctgatt ttctggctgc cttacagcca gccgctgttt gctgccttgt
    28681 tcccacaact gccacgaccc gtttatcagc aagaaagttt tgcagctctg gcactggctc
    28741 atttctggct ggtgggaatt tcgagtttgt ttgcggtgat cattggcaca ggtgccggaa
    28801 ttgctgttac tcgcccgtgg ggcgcggaat ttcgcccact ggtggaaact attgccgccg
    28861 ttggacagac ttttccgcct gtcgcagtgc tggcgattgc cgttccggtg atcggctttg
    28921 gtctgcaacc agcgattatc gccttgatcc tttacggtgt gctgcccatc ctgcaggcga
    28981 cacttgccgg gctgggagcg attgatccca gcgtgacaga agttgcgaaa ggtatgggaa
    29041 tgagtcgagg tcagcgactg cgtaaggtcg agctaccgct ggcggctccg gtgattctgg
    29101 cgggcgtgcg aacttcggtg attatcaaca ttggtacggc gacgatcgcc tcaacggtag
    29161 gggccagtac gctgggtacg cccatcatca tcgggcttag cggatttaat accgcgtatg
    29221 tgatccaggg ggcgttactg gtggcactgg cggcgatcat cgcagaccgc ctgtttgaaa
    29281 ggctggtgca ggcgcttagc cagcacgcaa aataaaggta taacctgcga gcatgacgcc
    29341 accaattccg cctaacgcca taaacaggaa cagggcgatg accccaattt tagctatgcg
    29401 cataatgcac tccttatgtt aacgaaagga ttgtacagta aagcgcattt gttaacgaat
    29461 cattaaatac cgagtgggaa aatatcatgg ccttgttctt gccaactggt gagttgctgc
    29521 tgttgggcgg agtgaaccgc cccgggtttc ctggagagtg ttttatctgt gaa
//
LOCUS       DADLSE010000039        29129 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLSE010000039 DADLSE010000000
VERSION     DADLSE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.039"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..846)
                     /gene="gsiB"
                     /locus_tag="JN692_001704"
                     /pseudo
     CDS             complement(1..846)
                     /gene="gsiB"
                     /locus_tag="JN692_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
     gene            complement(866..2737)
                     /gene="gsiA"
                     /locus_tag="JN692_001705"
     CDS             complement(866..2737)
                     /gene="gsiA"
                     /locus_tag="JN692_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7264036.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQDPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEYAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCEVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(2724..3689)
                     /gene="iaaA"
                     /locus_tag="JN692_001706"
     CDS             complement(2724..3689)
                     /gene="iaaA"
                     /locus_tag="JN692_001706"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7264037.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            3893..5128
                     /gene="moeA"
                     /locus_tag="JN692_001707"
     CDS             3893..5128
                     /gene="moeA"
                     /locus_tag="JN692_001707"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7264038.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            5128..5877
                     /gene="moeB"
                     /locus_tag="JN692_001708"
     CDS             5128..5877
                     /gene="moeB"
                     /locus_tag="JN692_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7264039.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(6056..6718)
                     /gene="fsa"
                     /locus_tag="JN692_001709"
     CDS             complement(6056..6718)
                     /gene="fsa"
                     /locus_tag="JN692_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7264040.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            6849..7748
                     /locus_tag="JN692_001710"
     CDS             6849..7748
                     /locus_tag="JN692_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7264041.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLPAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            7754..10186
                     /locus_tag="JN692_001711"
     CDS             7754..10186
                     /locus_tag="JN692_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7264042.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            10332..11147
                     /gene="ybiV"
                     /locus_tag="JN692_001712"
     CDS             10332..11147
                     /gene="ybiV"
                     /locus_tag="JN692_001712"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7264043.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            11299..12564
                     /locus_tag="JN692_001713"
     CDS             11299..12564
                     /locus_tag="JN692_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7264044.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPNRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(12805..14397)
                     /locus_tag="JN692_001714"
     CDS             complement(12805..14397)
                     /locus_tag="JN692_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7264045.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            14616..15536
                     /gene="ldtB"
                     /locus_tag="JN692_001715"
     CDS             14616..15536
                     /gene="ldtB"
                     /locus_tag="JN692_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7264046.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDLVVLDEAIKNRSGMPVRLN"
     gene            complement(15594..16712)
                     /locus_tag="JN692_001716"
     CDS             complement(15594..16712)
                     /locus_tag="JN692_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7264047.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAVGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(16709..17176)
                     /locus_tag="JN692_001717"
     CDS             complement(16709..17176)
                     /locus_tag="JN692_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7264048.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            17362..17490
                     /gene="mntS"
                     /locus_tag="JN692_001718"
     CDS             17362..17490
                     /gene="mntS"
                     /locus_tag="JN692_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7264049.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            17762..19348
                     /locus_tag="JN692_001719"
     CDS             17762..19348
                     /locus_tag="JN692_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7264050.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETSFVQSKETSCYL
                     YTMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQV
                     PFMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITN
                     FQLQKVDYNHLGTDIFDPKP"
     gene            complement(19396..19911)
                     /gene="ompX"
                     /locus_tag="JN692_001720"
     CDS             complement(19396..19911)
                     /gene="ompX"
                     /locus_tag="JN692_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7264051.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(19964..20029)
                     /locus_tag="JN692_001721"
     CDS             complement(19964..20029)
                     /locus_tag="JN692_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264052.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            20264..21151
                     /gene="rhtA"
                     /locus_tag="JN692_001722"
     CDS             20264..21151
                     /gene="rhtA"
                     /locus_tag="JN692_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7264053.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            21450..21953
                     /gene="dps"
                     /locus_tag="JN692_001723"
                     /gene_synonym="pexB"
     CDS             21450..21953
                     /gene="dps"
                     /locus_tag="JN692_001723"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7264054.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            22357..23103
                     /gene="glnH"
                     /locus_tag="JN692_001724"
     CDS             22357..23103
                     /gene="glnH"
                     /locus_tag="JN692_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7264055.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            23242..23901
                     /gene="glnP"
                     /locus_tag="JN692_001725"
     CDS             23242..23901
                     /gene="glnP"
                     /locus_tag="JN692_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7264056.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            23898..24620
                     /gene="glnQ"
                     /locus_tag="JN692_001726"
     CDS             23898..24620
                     /gene="glnQ"
                     /locus_tag="JN692_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7264057.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVIQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            24881..27106
                     /gene="ybiO"
                     /locus_tag="JN692_001727"
     CDS             24881..27106
                     /gene="ybiO"
                     /locus_tag="JN692_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7264058.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRVPVQTYQVLPAPGAPPAEPLPPGEPT
                     L"
     gene            complement(27103..28029)
                     /gene="rlmF"
                     /locus_tag="JN692_001728"
     CDS             complement(27103..28029)
                     /gene="rlmF"
                     /locus_tag="JN692_001728"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7264059.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            28305..28565
                     /locus_tag="JN692_001729"
     CDS             28305..28565
                     /locus_tag="JN692_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7264060.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            28830..>29129
                     /locus_tag="JN692_001730"
     CDS             28830..>29129
                     /locus_tag="JN692_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7264061.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         7018 a         7767 c         7305 g         7039 t
ORIGIN      
        1 ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
       61 gccatcaact cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg
      121 aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
      181 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
      241 ttcaccttca caaaatcggt ctgattccag gtatccagtt catactggtc cggttcccac
      301 cggatgaaaa ccaatctcct tgccatattt ttccagcgct gtcggtgaaa tcatcgcggt
      361 cgccggatgg gcaagaatat taataaacgc tgagaacggc tgtttgaggg taatctttac
      421 cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat agcgtttaag
      481 atgattcgcc ggatcgctgg cccggtccag attcgctttt accgccactg cgttgaaatc
      541 ggtgccatcc tggaatttaa ttccttcccg caatttcacg gtgtaagtaa tgccgtcatc
      601 ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat ccagaccgaa
      661 cagcccctag taaaacgatt tcgctacggc ctgagataac gtgtcattcg cgtcatacgg
      721 atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag cgaatgcaca
      781 agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt gtacagctct
      841 tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct gattgtggtt
      901 gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc tcttcgccac
      961 gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt cgttgtcggg
     1021 acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc tgcgggtttt
     1081 cgaagacctc gcaccgtgaa ccaatttcaa caatttgccc gagatacatc accgccacac
     1141 gatgactaat ccgctctacc acagccatat cgtgggagat aaacagatac gcaatgccga
     1201 aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa acatccagcg
     1261 ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg cgagcaatgc
     1321 agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca tattcaggta
     1381 acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta cctggcaata
     1441 aaccgtgtac acgcagcggt tcgataatcg aatcaccgat ggtctgacgt gggtccagcg
     1501 aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga agtttgccag
     1561 gtgacaaggt atcgattcgc tgaccgttaa agataatttc gccgccctgc gattcgacca
     1621 ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc accagcgata
     1681 gcgtttcgcc aggccagaga tcaaaactga ctttctcaac ggcatgcact tcccgcgtta
     1741 cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc acccgtaaaa
     1801 caggttcgcc atccaccacc gttttctgct cgatgggggg gtcctgtttc gctggatgtt
     1861 caagcgatat caatgggaaa cgtcggggat aatctaaccc tttcatcgca ccaagttgcg
     1921 gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa atctgttcga
     1981 cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg gcaatctctg
     2041 ccaccacgcc catatcgtga gtgataaaga taacgcccat cgacatctct ttttgcaata
     2101 ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg gttggctcat
     2161 cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact cgctggcgca
     2221 tcccgccaga gagttgatgc gaataacgtg aaagaatggt ttgtgcctcc ggaatgcgta
     2281 cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg ttctgatgca
     2341 gacgaattga ttcggcaatc tgttcaccca cagtaaatac tgggttcagc gatgtcatcg
     2401 gctcctgaaa tatcatcgcc atatccgcac cgcgcacatg gcgcatttgt gcagcgctct
     2461 gctcgctaag ttcaatcaca tcacgactgc gccgccgcaa cagcatttta tcgcactgta
     2521 ctaaaccgcc cgcctgttcc aacagacgca tcaatgccag cgcagtcact gacttaccgg
     2581 agccggattc gcctacaatt gccagcgtct caccgcgttg cagactaaaa gagagattgc
     2641 ggaccgcagc tattttctgc tggtcctgca taaaggcaat attaagattt tcaaccgcca
     2701 gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt ttcacggtag
     2761 ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc ttcggtgtta
     2821 aacggtagcg cgacattccc ttcatggtcg atagcgatta agccaccgct accgccaagc
     2881 gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa tccgccgtaa
     2941 tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc gcctgtgccg
     3001 gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac taagggacta
     3061 tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc tgccgccaga
     3121 ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt ttcatccagt
     3181 ggcgcaccgc tatggtcgag gactgttgcc ccttcctcgc gcgctgccag tagttgttca
     3241 taacgcaaag gcgtggagaa aatctccggt gagacgcgct ccatgccatg agcaaacgca
     3301 aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat caccagccgg
     3361 gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc tttcagggtg
     3421 ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa gacagcgcca
     3481 attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt caccacatcc
     3541 agcgcacttt cgccggcttc cagcattttc tgcccggttt caacaatggc agacaacgcc
     3601 tcgatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc acctgcgcca
     3661 ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg gctcgccact
     3721 tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat atagaaagat
     3781 ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac agtctatggc
     3841 gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt tcatggaatt
     3901 taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc gcgttacccc
     3961 actgaccacc caggaaacgc tgccactggt tcagtgtttt ggtcgtattc tggcgagcga
     4021 tgtcgtttcg ccactggatg tcccggggtt tgataactcc gctatggacg gctacgcggt
     4081 gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt gccggtaaat cctttgccgg
     4141 tcagccatac catggtgaat ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc
     4201 ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa cggacaatgg
     4261 cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat
     4321 ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag agctgccagt
     4381 aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt
     4441 ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggcgacggcc aaatctacga
     4501 caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg taatcaactt
     4561 agggattatc cgcgacgatc cacatgccct gcgcgccgca tttattgaag ccgacagcca
     4621 ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac
     4681 gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac caggtaaacc
     4741 gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca acccggtttc
     4801 agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa gcggcaacac
     4861 cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc
     4921 aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt
     4981 gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg gcaactgctt
     5041 tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg aagtagaacc
     5101 gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga gatgctgcgc
     5161 tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc gctaaaagat
     5221 tctcgtgtgc tggtagtcgg cctcggtgga ctcggctgcg cagcttcgca gtatctggca
     5281 agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct ctcgaatctg
     5341 caacgccaga cactgcacag tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt
     5401 gacgccctga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc actgctagat
     5461 gacgcagaac ttgcagcatt gattgctgaa cacgatctgg tgctcgactg tacggataac
     5521 gttgcggtac gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc
     5581 ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga cggcgaaccg
     5641 tgctatcgct gccttagccg tttgtttggc gagaacgcgt taacctgcgt agaagcaggc
     5701 gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc gatcaaactg
     5761 ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc gatgacctgt
     5821 cagtttcgcg aaatgaaact gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg
     5881 ccacctccag gcctgataag acgcgacaag cgtcgcatca ggcatttgct ccagacgcca
     5941 gatgcggcgt gaacgcttta tctggcctac ggttcgggca cagatttcca agcgcggcag
     6001 gcatcggtgc actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa
     6061 tcgacgttct gccaaacgct ccctgccagt cctgctcaaa cttcgccaca gcggcttcca
     6121 ccgccggata gctaatcatc tgttgtgcca catccagtgg cagagtaatt gattcacatc
     6181 ctgccagtaa gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg
     6241 cctgcggcgc atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac
     6301 cgccctgagc atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg
     6361 acagcagccc ttgtgccgcg ccatataccg cggttcccag cgtcggaatc ccttccgctt
     6421 ttaacatctt aatcgctgcc agcccctcgg ctgtcaccgg aactttcacc acgatatccg
     6481 caataataga acgcagctta agcgcgtcat tcaccatccc ttcagcagtg gtagccatta
     6541 cctgggcaaa cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat
     6601 ccagcggttt tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa
     6661 aaatacgtga cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa
     6721 ccatcctcaa aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca
     6781 gttgacgcac atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cgaaacagag
     6841 aggcaactat gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca
     6901 cggtcgtatt tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc
     6961 gcgcccgtac gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt
     7021 gcgctaaggc cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg
     7081 aaaagttaac cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcgttaa
     7141 ccgtgtgtgg tgaagtgaaa agcgttgagg agatcatggc gaccgttctg cgcgataaac
     7201 cgttttacga tcgcagcggc ggcggcttaa cgctttcggg tggtgaaccc ttcatgcagc
     7261 cggaaatggc gacggcgctg ctgcaagcct gccacgaggc aggcattcat actgcggtag
     7321 aaacctgtct gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt
     7381 ttcttgccga tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg
     7441 ccgccagggt gctggataac ctgaaaaaac tccccgcagc gggcaaaaaa atcattatcc
     7501 gcgtgccgct gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt
     7561 ttgccgccga cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca
     7621 tcaacaaata tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc
     7681 cagagctgct cgattttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac
     7741 gaggataaca accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca
     7801 caaaaatgcg ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta
     7861 taccgagatg tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact
     7921 ggcgcatcac ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa
     7981 ccaggcaagc gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga
     8041 aaaagagatt gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa
     8101 caaacgtgtt ctgcatgaag tgtgcccgtg gtggcgcggt cagacggtac aggatcgctg
     8161 ctatggcatg tttaccgatg agcaaaaagg tctgctggcg accggcatca ttaaagcgga
     8221 aggcaatatg acctccggcg atgcgcacct ggcggtcaat ttcccgctgc tgctggaaaa
     8281 agggcttgat ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt
     8341 gctggaagat ttgcacggcg aacagttcct gaaagcgatt gatatcgtgc tggtggcagt
     8401 cagtgaacac attgaacgtt tcgctgccct ggcgcgtgca atggccgcga ccgaaacccg
     8461 cgaaagccgt cgcgatgaac tgctggcgat ggcagaaaac tgcgatctta tcgcccacca
     8521 gccgccgcag actttctggc aggcgctgca actgtgttac ttcatccagt tgattttgca
     8581 gattgaatct aacggtcact cggtgtcgtt tggtcgtatg gatcagtatc tctacccgta
     8641 ctatcgccgc gacgttgaac tgaaccagac gctggatcgc gaacacgcca tcgaattgct
     8701 gcatagctgc tggttgaaat tgctggaagt gaacaaaatc cgctccggct cccactcaaa
     8761 agcctctgcg ggaagtccgc tgtatcagaa cgtcaccatt ggcggacaaa atctggttga
     8821 tggtcaacca atggacgcgg tgaatccact ctcttacgcg atcctcgaat cctgcggtcg
     8881 cctgcgttcc actcagccta acctcagcgt gcgttaccat gcaggaatga gtaacgattt
     8941 cctcgacgcc tgcgtacagg tgatccgctg cggcttcggg atgccggcat tcaacaacga
     9001 cgaaatcgtg atcccggaat ttattaaact cggtattgaa ccgcaggacg cttatgacta
     9061 cgcagcgatt ggttgtatag aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg
     9121 catgagcttt atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc
     9181 caccagcggc aaagtgttcc tgccacaaga aaaagcgttg tcggcaggta acttcaacaa
     9241 cttcgatgaa gtgatggacg cgtgggatac gcaaatccgt tactacacgc gtaaatcaat
     9301 cgaaatcgaa tatgtcgtcg acaccatgct ggaagagaac gtgcacgata ttctctgctc
     9361 ggcgctggtg gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata
     9421 tgactgggtt tctggcctgc aggtcggtat tgccaacctc ggcaacagcc tggcggcagt
     9481 gaagaaactg gtgtttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc
     9541 ggatgacttc gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc
     9601 gaagtacggc aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta
     9661 tatcgacgaa ctgaaacagt accataaccc acgctacggt cgtggtccgg ttggcggcaa
     9721 ctattacgcg ggtacgtcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc
     9781 aacgccggac gggcgtaaag cccacacccc gctggcagaa ggcgctagtc cggcctctgg
     9841 tactgaccat ctcggcccta ctgcggtcat tggctcagtg ggtaaactgc ctacggcagc
     9901 gattctcggc ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc
     9961 tgacaagcag aaactgatga tcctgctgcg taccttcttt gaagtgcata aaggctggca
    10021 tattcagtac aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca
    10081 gtaccgcgat ctggtagtac gtgtcgcggg ttattccgcc ttcttcaccg cgctctctcc
    10141 agacgctcag gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ttcggcccgg
    10201 ctgcggcgtc gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa
    10261 atataatttg tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca
    10321 ggagcacacc tatgagcgta aaagttatcg tcacagatat ggacggtact tttcttaacg
    10381 acgccaaaac gtacaaccaa ccacgtttta tggcgcaata tcaggaactg aaaaagcgcg
    10441 gcattaagtt cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg
    10501 agctaaagga tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca
    10561 agcaactgtt ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc
    10621 taaaagataa gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa
    10681 atgcccccga agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaacctgtaa
    10741 aagattatca ggagattgac gacgtactgt tcaagttttc gctcaacctg ccggatgaac
    10801 aaatcccgtt agtgatcgac aaactacaca tagcgctgga tggcattatg aaacccgtca
    10861 ccagtggttt tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt
    10921 cgcggttact gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg
    10981 gtaacgatgc ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg
    11041 aaaacattaa acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga
    11101 atgtgattca ggctgtgctg gataacacat ccccttttaa cagctgacct ctctaccgga
    11161 gccatgcgct ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac
    11221 ttgcattaac ttatttataa cttgtattgt cacatgcaaa tcagcatagt ttcgaatgaa
    11281 agttaatgcg aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac
    11341 aaagcagcta tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga tgtccagcag
    11401 atctttaatc agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa
    11461 gcacagggcg atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt
    11521 actgcagagc agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc
    11581 ccccgcgaac aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc
    11641 tttgacgagg tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt
    11701 cccgagattt accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa
    11761 atggcgaatg cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa
    11821 tggtttaacc cggatgtgag cgtcatctac cctaaccgta tccgccgcct tccgcccgga
    11881 acgacctcca aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt
    11941 ccagcatatc agcacgtttt cgccaacgtc tttaatggca atctggcgaa atacgatccc
    12001 tggcatgcgg cacatcgtac ggaagttgaa gagtacacgg tggacaacac taccaaatgt
    12061 tccgtgtttc ggacattcca gggctggact gcgctctcgg atatgctgcc tggtcagggg
    12121 ttgctgcacg tcgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt
    12181 gatgatgtgc cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca
    12241 gagcaatggc atccactgtt aattgaggcg ttaaccagca ttccaaaact cgaggccgga
    12301 gactccgtct ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag
    12361 ggttggggca acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac
    12421 gcgcacaagg tgaaggccgc actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag
    12481 gactatgaaa caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag
    12541 cgagcgttgg gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa
    12601 atcgattcga cgttactcag attgctgaca agtgcgcttt cttaatgcct gatgcggcgt
    12661 taacgcctta tccggcctac aaagtcctgc agattcaata tattacaggt actgcgtagg
    12721 cctgataagc gtagcgcatc aggctatttt gcatttgtca tcagccgtcg tcaccccgac
    12781 agtatcgggg tgacggataa tcacttactc gatcccttta ctacgcaggt aatcttcgta
    12841 attaccgcta aagtcgatca cgcgttccgg ggtgatttcc agaatgcggg tcgccaggga
    12901 gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca gttccagcgc
    12961 catgttcagt gactcgatgg attccatatc caggtggttg gtcggttcgt ccatgatcag
    13021 aatgttcggc ttctgcatca ttaacttacc aaacagcatc cgcccttttt ccccaccgga
    13081 aagcacttta gctggctttt tgatgtcgtc ctggctgaac agcaaacgac cgagaatgct
    13141 gcgtaccgcc tgctcgtcat cgccttcctg cttccactga ctcatccatt cgaacacggt
    13201 cagatcattt tcaaactcat attcgtggtc ctgggcgtag taaccaatgc gcgcgttttc
    13261 agaccatttt acggtgccgc tgtccggttg cagatcgccc accagcgttt tcagcagcgt
    13321 tgatttaccg acgccgttgg tacccagtac cgcaagtttt tcaccgacct ccagcagcag
    13381 gttgagattt ttaaacagtg gaccgttatc aaaccctttg gtcagacctt ccacttccag
    13441 cgcgttacgg aacagtttct tatcctgttc aaaacggatg aacgggttct gacggctgga
    13501 ggctttcacc tcttccagtt tgattttatc aatctggcgc gcgcgcgaag ttgcctggcg
    13561 agatttcgag gcgttggcgc taaagcggct aacgaaagat tgcaactcag caatctgcgc
    13621 tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc tgggtcgccg ccgtcatgta
    13681 ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa tccagatccg ccatgtgggt
    13741 acagaccatg ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc tgtcacgctc
    13801 gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg tcggttcgtc
    13861 gagcaggaga atatccggct cagcaaacag cgcctgcgcc agaagcacac gcagcttcca
    13921 gccaggagca acttcactca tcgggccgta gtgctgttcc actggaattc ccacgccaag
    13981 caacagttca ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt atttcacttc
    14041 cagatcggcc actttatagc cgtcttcttc actcatttcc ggcaaagcat agatgcggtc
    14101 gcgctcctgc ttcacttccc acaactcttt atgccccatg atcaccgtat ccagcacagt
    14161 gaactcttca aaggcaaact gatcctgacg cagcttacca atgcgctcgt tgggatcgag
    14221 ggaaacgtta cccagcgtcg gctcaaggtc gccgccgaga atcttcataa aggtggattt
    14281 accactaccg ttcgcgccaa tcaggccgta acggttgccg ccgccgaatt tgacggaaat
    14341 gttttcaaat aacggcttac tgccgaactg catggtgacg ttactggaaa ctaacacagg
    14401 cgtatcctga aaagagatat gacaaaccgc gcattatgcc ataagtctga aagaagatcc
    14461 tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt cgtacacatc
    14521 tgatttcact gtgagctgga atgaacttat aatgcgcttc caatactcta atattctcaa
    14581 cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg aaaacattat
    14641 tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta acttatcctc
    14701 tgccaaccga cgggagtcgc ctggttggtc agaatcaggt gatcaccatt cctgaaggta
    14761 acactcagcc gctggagtat tttgccgcgg agtaccagat ggggctttcc aatatgatgg
    14821 aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg tactgtactg aacattccgc
    14881 agcagctgat cctgccggat accgttcatg aaggcatcgt cattaacagt gcagagatgc
    14941 gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc ggcattggtc
    15001 agttaggcaa agatacgcct atcaactgga ccaccaaagt tgagcataag aaagcaggcc
    15061 cgacctggac gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc gaaccgctac
    15121 cggctgtcgt tccggcaggt ccggataacc cgatgggtct gtatgcactc tacatcggtc
    15181 gcctgtatgc tatccatggc accaacgcca acttcggtat cggcctgcgt gtaagtcatg
    15241 gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt cgagaaagta ccggtcggta
    15301 cccgcgtaca gtttattgat gagccggtaa aagcgaccac cgagccagac ggcagccgtt
    15361 atattgaagt ccataacccg ctgtctacca ccgaagccca gtttgaaggt caggaaatcg
    15421 tgccaattac cctgacgaag agcgtgcaga cagtgaccgg tcagccagat gttgacctgg
    15481 ttgttcttga tgaagcgatc aaaaatcgtt ccgggatgcc ggttcgcctg aattaatcgt
    15541 caaaacgtaa aacaaaggcg gactcataat ccgccttttt tatttgccag accttaccag
    15601 gccgggatca taactaacaa aacgtatccc accagcgccg cccacaacag catcggtatt
    15661 gaatagagat ggaaacgcca ccagatgcgg cgatcgttcg ccatacgtag cgcaatcaaa
    15721 tttgccagcg atcctggtaa taaaccaaag ccccccacgt ttaccgccca tgccagtaat
    15781 aaagacggag gtacataatt gagcagcaaa atggtactcg gcacgttact gatcacctgc
    15841 gataaaccga ctgccgttaa ccataacccg ggttcagata aatgactcac gttacccaac
    15901 acgccttgca acgctggaag ctgggtcagt aaatggacgt cgataaacat cgccataaac
    15961 accagcagca acgtccagtc cacactcaac accacgcgac gcgccagcag cacaaagccc
    16021 accgccacaa tcaccagtcc ccacagctct tgtttgaact ccagcgccgt cagaaagacg
    16081 atatacagcc ccagacaatt ccacaccagc cgcggtttcc actccggtgt ttgcaccccc
    16141 gtatggtatt gcagcgcctt tccagggaaa caacaccagc acaacagcag gagtgtcagc
    16201 atcattgcgc cagccagcgg tgccatttgg gcaataaatc cggcaaacga aagaccagaa
    16261 cgtccccaga taagaatatt ttgcgggttg ccaattggcg tcagtagcga accagcgttg
    16321 actgccagcg cctcaaaaat aatcagccga ttaaccggga tctcacacag tctttttagc
    16381 gtgatagtca gcggaacaac aataaacagc gcgacatcgt tggtcagaaa ggtagaaagc
    16441 agcgccgccg ccagcaccat aaacatcgcc agccgacgct ccgtagcaaa gcggcgcacc
    16501 attttgcgcc ccagcacatc aaaataaccg cttaactcca cacctttggt cagcagcatc
    16561 aggccgctta aggtgatgat ggtgtgccag tcgatagcag caggccagga tttcggtgca
    16621 aagggcacga aaaagcttaa tccgatacca acaagaatta ataactgaaa aaaacgatcg
    16681 ccttgcagcg tgcgtaaaaa aggcaggctc atttggcacc gtgtttctgg gtaaacaaac
    16741 gaaaagcgtc gagcgtctct tcactaacat ggtgctccat gccttccgcg tcgcgacggg
    16801 cgatttccgg actgacgccc aacaccaaca agaaattttc gactatctga tgtcgctcgc
    16861 ggctttcctg cgccagcttc tctccttctg ccgttaaaaa cacgccacgc caggggatca
    16921 tttcaatcag ccccatggtt gccagccgct taagcatttt agccaccgtc ggttgcgaaa
    16981 ctcccagacg agcagccatg tccacctgac gagcttcccc cacttccctg atcaagtcag
    17041 aaatcagctc aacgtaatca tcaataagct cgcgccgatg cgcctctctg acctggcgga
    17101 acccttcaac gtgctcttcc acgttcacta actgcgtcac tttttttgct gttggcgtac
    17161 ctgcgcgacg actcattgtg cttcctcatt atggtgacgc atacatagcg ttctcatttc
    17221 gtgcgcacat tgtaaaccag agttgcgaag gtacaaaaaa ttaacgtttt agcaatagct
    17281 atataatata gcctgtgcta tatctgtatg taatgcaatc atccctcaag gatcgacggg
    17341 attagcaagt caggaggtct tatgaatgag ttcaagaggt gtatgcgcgt gtttagtcat
    17401 tctcccttta aagtacggtt aatgctgctc tctatgttgt gcgatatggt caacaacaaa
    17461 ccgcagcaag ataaaccttc agataaatag cggcgtcgcg gtacgccgct tcactcctgc
    17521 ttttatgcag gcataacgcg ttttggtctg aaaaaaccca ctttttgtcg gatttgcaat
    17581 ccccttcgca aaagatttgt tcgtcagtag ttgacctgaa cggcggctcg ctctatcttc
    17641 ttgcagccct gcgtatattg cggctcgcgg atgcggaccc ctttcccctc ttcacgcact
    17701 cttgcaggta ttgacccttg acgccagggt aagcacatgg cgtttgttac gatagtggca
    17761 tatgaattta accctcaaag aatcgcttgt tacccgtagc cgggtattta gcccgtggac
    17821 tgcgttctac tttttacagt cgctattaat taacctcggc ttaggttacc ccttcagttt
    17881 gctctacacc gctgcgttta cggctatttt gcttttgcta tggcgaacat tgcctcgcgt
    17941 acaaaaagtt ctggtcggtg tcagttcgct ggtggcggct tgttatttcc cttttgctca
    18001 ggcctacggc gcgcctaatt tcaatacatt gctggcattg cactccacca atatggaaga
    18061 gtcgaccgaa atcctgacga tttttccgtg gtacagctac ctggtcggct tatttatttt
    18121 tgcgctcggc gtaatagcaa tcaggcgaaa aaaagagaat gaaaaagcgc gctggaatac
    18181 cttcgacagc ctgtgtctgg tattcagtgt ggcgacattt tttgttgctc ccgtgcaaaa
    18241 cctggcctgg ggtggcgtat ttaaactgaa agatactggc tatccggtat ttcgttttgc
    18301 taaggatgtc atcgtcaata ataacgaggt gattgaagag caagaacgga tggcaaaact
    18361 ttccggaatg aaagatactt ggacggtcac tgccgttaag ccgaagtatc agacctatgt
    18421 ggtagtgatc ggtgaaagcg cgcgtcgcga tgccctcggt gcctttggcg gtcactggga
    18481 caataccccg tttgccagca gcgttaacgg tttgatattt gctgactaca ttgccgccag
    18541 tggctccacg cagaaatcgc ttggcttaac gctcaatcgc gtagtcgatg gcaaaccaca
    18601 gtttcaggat aactttgtca ccctggcaaa tcgcgcgggc ttccagacct ggtggttttc
    18661 caaccagggc caaatcggcg aatacgatac tgctatcgcc agcatcgcca aacgtgcaga
    18721 tgaagtgtac ttcctgaaag aaggtaattt tgaagcagat aaaaacacga aagacgaagc
    18781 gttactggat atgaccgctc aagtgctggc gcaagagcac tcgcaaccgc agctgattgt
    18841 tctgcatctg atgggctcgc atccgcaggc ctgcgacagg acacaaggca aatacgaaac
    18901 ctcctttgtg caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga
    18961 tttactgcgc aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta
    19021 cttttctgac cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca
    19081 tgatgataaa tatcagcaaa atttccaggt gccttttatg gtcatttcca gcggcgataa
    19141 agcgcatcgg gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca
    19201 gtggacggga attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa
    19261 agccgggccg atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg
    19321 aaccgatatt ttcgacccga aaccttaaaa acaaaaatcc gccccgagag gcggattttt
    19381 atatcaccaa agtgattaga agcggtaacc aacaccggca atccaggtgc ctacgtcaac
    19441 gctacgaata cggctctgct cgtaagagaa gtccagagca acgttttcca tcgggttgaa
    19501 ctgcagacca gcaccgtagg agaaaccgta gtcgctggtg tcgtgtttgt aggtcgggta
    19561 ttcagtggtc tggaatttac cataacccac acccactaca ccgtagatgc ttgcccagtc
    19621 gttaatgcgg taagccggac cagcagtgat gccatagtac tggtttttgt tgtagtcacc
    19681 agagcttgca gtacggcttt tctcggtgta agtgaaagaa ccgatcacac ccagcgggct
    19741 gttgtcttct tcatagcggt atttcaggtt gaaaccgccc attttgttca tttggccctg
    19801 agcgtcgctc tgtgcgtaac cgccagttac ggtagaagtc gcagctacgg aagtacctgc
    19861 ggtgaaagcc agaactgcgg ccagtgctga aagacatgca atttttttca taaccacctc
    19921 aaatgtgatt caaataagtc ctaagtttta aatatatcaa aaattaatgg gaaactcttc
    19981 gcgatttgtg atgtctaacg ggccatttca tgtaacagaa cgtttccata caccgctatc
    20041 catctaaatt taaatcactt tttcagagaa ctgcgtaagt attacgcatg ttttccctgt
    20101 cattcatcca gattattcct aatcaccaga ctaatgattc catcaatcct ggcgcatttt
    20161 agtcaaaacg ggggaaaatt ttttcaacaa atgctcaacc agcattgggt atatccagta
    20221 cactccacgc tttacttaag tctagatatt tgtgggagaa aggatgcctg gttcattacg
    20281 taaaatgccg gtctggttac caatagtcat attgctcgtt gccatggcgt ctattcaggg
    20341 tggagcctcg ttagctaagt cactttttcc tctggtgggc gcaccgggtg tcactgcgct
    20401 gcgtctggca ttaggaacgc tgatcctcat cgcgttcttt aagccatggc gactgcgctt
    20461 tgccaaagag caacggttac cgctgttgtt ttacggcgtt tcgctgggtg ggatgaatta
    20521 tcttttttat ctttctattc agacagtacc gctgggtatt gcggtggcgc tggagttcac
    20581 cggaccactg gcggtggcgc tgttctcttc tcgtcgcccg gtagatttcg tctgggttgt
    20641 gctggcggtt cttggtctgt ggttcctgct accgctgggg caagacgttt cccatgtcga
    20701 tttaaccggc tgtgcgctgg cactgggggc cggggcttgt tgggctattt acattttaag
    20761 tgggcaacgc gcaggagcgg aacatggccc tgcgacggtg gcaattggtt cgttgattgc
    20821 agcgttaatt ttcgtgccaa ttggagcgct tcaggctggt gaagcactct ggcactggtc
    20881 ggttattcca ttgggtctgg ctgtcgctat tctctcgacc gctctgcctt attcgctgga
    20941 aatgattgcc ctcacccgtt tgccaacacg gacatttggt acgctgatga gcatggaacc
    21001 ggcgctggct gccgtttccg ggatgatttt cctcggagaa acactgacac ccatacagct
    21061 gctggcgctc ggcgctatca tcgccgcttc aatggggtct acgctgacag tacgcaaaga
    21121 gagcaaaata aaagaattag acattaatta aatttacatt tctgtatggt tatgcataac
    21181 catgcagaat ttctcgctac ttttcctcta caccgtcttt atatatcgaa ttatgcaaaa
    21241 gcatatttat tccgaaaatt cctggcgagc agataaataa gaattgttct tatcaatata
    21301 tctaactcat tgaatcttta ttagttttgt ttttcacgct tgttaccact attagtgtga
    21361 taggaacagc cagaatagcg gaacacatag ccggtgctat acttagtctc gttaattact
    21421 gggacataac atcaagagga tatgaaatta tgagtaccgc taaattagtt aaatcaaaag
    21481 cgaccaatct gctttatacc cgcaacgatg tctccgacag cgagaaaaaa gcaacagtag
    21541 agttgctgaa tcgccaagtt atccagttta ttgatctttc tttgattacc aaacaagcgc
    21601 actggaacat gcgcggcgct aacttcattg ccgtacatga aatgctggat ggcttccgca
    21661 ccgcactgat cgatcatctg gataccatgg cagaacgtgc cgtgcagctg ggcggtgtag
    21721 ctctggggac cactcaagtt atcaacagca aaaccccgct gaaaagttac ccgctggaca
    21781 tccacaacgt tcaggatcac ctgaaagaac tggctgaccg ttacgcaatc gtcgctaatg
    21841 acgtacgcaa agcgattggc gaagcgaaag atgacgacac cgcagatatc ctgaccgccg
    21901 cgtctcgcga cctggataaa ttcctgtggt ttatcgagtc taacatcgaa taaatccatc
    21961 gctgatggtg cagaacttta gtacccgata aaagcggctt cctgacagga ggccgttttg
    22021 ttttgcagcc cacctcaacg cacttattta gtgcatccat ctgctatctc cagctgatta
    22081 agtaaatttt ttgtatccac atcatcacac aatcgttaca taaagattgt tttttcatca
    22141 ggttttacgc taaataatca ctgtgttgag tgcacaattt tagcgcacca gattggtgcc
    22201 ccagaatggt gcatcttcag ggtattgccc tataaatcgt gcatcacgtt tttgccgcat
    22261 ctcgaaaaat caatgagttg caaaactggc acgatttttt catatatgtg aatgtcacgc
    22321 aggggatcgt cccgtggata gaaaaaagga aatgctatga agtctgtatt aaaagtttca
    22381 ctggctgcac tgaccctggc ttttgcggtt tcttctcatg ccgcggataa aaaattagtt
    22441 gtcgcgacgg ataccgcctt cgttccgttt gaatttaaac agggcgataa atatgtgggc
    22501 tttgacgttg atctgtgggc tgccatcgct aaagagctga agctggatta cgaactgaag
    22561 ccgatggatt tcagtgggat cattccggca ctgcaaacca aaaacgtcga tctggcgctg
    22621 gcgggcatta ccatcaccga cgagcgtaaa aaagcgatcg atttctctga cggctactac
    22681 aaaagcggcc tgttagtgat ggtgaaagct aacaataacg atgtgaaaag cgtgaaagat
    22741 ctcgacggga aagtggttgc tgtgaagagc ggtactggct ccgttgatta cgcgaaagca
    22801 aacatcaaaa ctaaagatct gcgtcagttc ccgaacatcg ataacgccta tatggaactg
    22861 ggcaccaacc gcgcagacgc cgttctgcac gatacgccaa acattctgta cttcatcaaa
    22921 accgccggta acggtcagtt caaagcggta ggtgactctc tggaagcgca gcaatacggt
    22981 attgcgttcc cgaaaggtag cgacgagctg cgtgacaaag tcaacggcgc gttgaaaacc
    23041 ctgcgcgaga acggaactta caacgaaatc tacaaaaaat ggttcggtac tgaaccgaaa
    23101 taataacgct acacctgtaa agcgcactgg cagttccctc tcccccctgg ggagaggatt
    23161 agggtgaggg gcgcaaaccc gctccggggc cattaattac cctgaatttg attatttaca
    23221 ccacggtaac aggaacaaca tatgcagttt gactggagtg ccatctggcc tgccattccg
    23281 cttctgattg aaggtgccaa aatgaccctg tggatttcgg tcctcggtct ggctggcggt
    23341 ctggtaatcg gattgctggc aggttttgca cgcaccttcg gtggttggat agccaaccac
    23401 gtcgcgctgg tctttattga agtgatccgc ggcacaccta tcgtcgtcca ggtgatgttt
    23461 atctatttcg ccctgccgat ggcgtttaac gacttacgca tcgacccatt tactgcggcg
    23521 gtggtcacca tcatgatcaa ctccggcgcg tacattgcgg aaatcacgcg tggtgcggtg
    23581 ctgtctatcc acaaaggttt tcgtgaagca ggactggcgc tcggtctttc acgttgggaa
    23641 accattcgct acgtcatttt accgctggca ctgcgtcgta tgctgccgcc gctgggtaac
    23701 cagtggatca tcagcattaa agacacctcg ctgtttattg ttatcggcgt ggcggaactg
    23761 acccgtcagg ggcaagaaat tattgccggt aacttccgcg cccttgagat ctggagcgcc
    23821 gtggcggtgt tctatctgat tattaccctg gtgctgagct ttattctgcg tcgtctggaa
    23881 agaaggatga aaatcctgtg attgaattta aaaacgtctc caagcacttt ggcccaaccc
    23941 aggtgctgca caatatcgat ttgaacattg cccagggcga agtcgtggtg attatcgggc
    24001 cgtccggttc cggtaaatcg accctgctgc gctgcatcaa caaactggaa gaaatcacct
    24061 ccggcgatct gattgtcgat ggcctgaagg ttaacgatcc gaaagttgac gagcgcctga
    24121 ttcgccagga agcaggtatg gtgttccagc agttttacct cttcccgcat ctgacggcgc
    24181 tggaaaacgt catgtttggt ccgctacgcg tgcgtggcgc gaacaaagaa gaggcggaaa
    24241 aactggcgcg tgagctgctg gcgaaagtcg gtctggcaga acgtgcacat cactaccctt
    24301 ccgaactttc tggtggtcaa cagcagcgtg tggcgattgc ccgcgcgctg gcggtgaagc
    24361 cgaaaatgat gctgtttgat gaaccgactt ccgctcttga cccggaactg cgccatgaag
    24421 tgctgaaggt tatacaggat ctggctgaag aagggatgac gatggtgatc gtgacccacg
    24481 aaatcggttt tgccgagaaa gtagcttcgc ggctgatctt tatcgacaaa ggccggattg
    24541 cggaagatgg caatccgcag gtgttgatca agaacccgcc gagccagcgc ttgcaggaat
    24601 ttttgcagca cgtctcttaa taagacacat tgcctgatgc gctacgctta tcaggcctac
    24661 aggatatctg gcaacttatt aaaattgcat gaacttgtag gacggataag gcgttcacgc
    24721 cgcatccggc aaaaaagccc gcacgttgtc agcaacctgc ttaatatccc ttcctccctt
    24781 tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc ccccagcatc
    24841 tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga tcctgttcat
    24901 cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg ttacaaccac
    24961 aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc
    25021 ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag agttgatcga
    25081 ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga tcgtgccgcc
    25141 gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg
    25201 tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct ccccgcataa
    25261 gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt tagcggtatt
    25321 agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg
    25381 tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat
    25441 tattggggcg tttattatcg acctgctgtt actggccctg acattatttg tcggccaagt
    25501 attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa gtttgtttct
    25561 caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt tttgcccaaa
    25621 cgtggcggag ctgcgcccgt tcacgattca ggacgagagc gcccgttact ggagtcgtcg
    25681 cctgagctgg ttaagcagcc tgattggcta tggcctgatt gtggccgtgc cgattatctc
    25741 taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac
    25801 cgtctgggcg ttgtacctga tcttccgaaa taaaaaagag attacccagc atttgctcaa
    25861 ctttgcggag cattcgctgg cttttttcag cctgtttatc cgcgcctttg cgctggtgtg
    25921 gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt tcgatccggg
    25981 caacagtctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc
    26041 agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc tctcgccaca
    26101 tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt cggcggcgct
    26161 gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga gcgcatgggg
    26221 attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct
    26281 gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc tcgccagttt
    26341 gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg cccgtacgcg
    26401 taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca tcaccatcat
    26461 gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt
    26521 aggtctggct atctcgtttg gttcgcaaac gctggtaaaa gatattatca ccggggtatt
    26581 tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc cgttgaccgg
    26641 cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg gggcgtatca
    26701 catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta ttggttcggt
    26761 agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc aggcactgaa
    26821 agatgcggta acggaattga tggaaaacga agaaattcgc gggctgatta ttggtgaacc
    26881 gaactttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg tttcgttcac
    26941 cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg tgaaaaaaca
    27001 tttcgacctg gcgggcgttc gcgtgccagt gcagacttat caggtgctgc ctgctccggg
    27061 cgctcccccg gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt ggcgattaac
    27121 aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac tctgcttttg
    27181 cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca gagcacgata
    27241 caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca cctgcttcgc
    27301 gaagcctttg ctctcttcaa tcatcttttt gataaagttg acttcaccgc cttcacacca
    27361 caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc ccaggttacg
    27421 gcgtttacgc tcactacctg cccgcgccgc agcggcggaa tcgtggaatg gcgggttaca
    27481 caaggtcgcg tcgtattgct cgtttttgtg gatgatgccg ttaaaaatcg ccccactctc
    27541 tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga taatcgcctg
    27601 cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc catattcatg
    27661 tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa tgctggcatt
    27721 cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa tataatccgc
    27781 ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg ctacggcgta
    27841 aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg caaagtctac
    27901 gctttgctcc cctgcgggtg taagcgtgag gaattgcctg agttcaggat tgacctgaca
    27961 aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg
    28021 ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac gcgacagggt
    28081 aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt ttatggcaat
    28141 tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta aaaatatatc
    28201 gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca tttgcatcgc gcaggcgacc
    28261 tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa aagtgcctca
    28321 cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc gcgcaaccga
    28381 tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca actggcgcat
    28441 caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgaacaa ggcgcgaaag
    28501 gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca accatctaca
    28561 aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct gttttgtaat
    28621 caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg cgcaatgggt
    28681 gatagaaaat cgcgtcaagt gataatgctt atcaaaatta ttatcacttg tgcgaacact
    28741 atcacgggat taacagtggc atcgcatccg cagagatgct ttctcgtggc agtgaaaatt
    28801 tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc cctgccagac
    28861 aatttcattc gctcacgttc tttgccggtc tttgtattgg tattacgcct gtggcacagg
    28921 cactcgccgc cgaagggcaa gctaacgcgg atgacacgct ggttgtcgaa gcatcaacgc
    28981 cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg gtagcggata
    29041 ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc gcaaccaacc
    29101 ttaccgacgc gctcaaaaac gtccccggc
//
LOCUS       DADLSE010000040        28471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLSE010000040 DADLSE010000000
VERSION     DADLSE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.040"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN692_001731"
     CDS             complement(<1..251)
                     /locus_tag="JN692_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7264062.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPVTLFVPLIMG"
     gene            561..2333
                     /gene="aspS"
                     /locus_tag="JN692_001732"
     CDS             561..2333
                     /gene="aspS"
                     /locus_tag="JN692_001732"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7264063.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2451..2903
                     /gene="nudB"
                     /locus_tag="JN692_001733"
     CDS             2451..2903
                     /gene="nudB"
                     /locus_tag="JN692_001733"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7264064.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAQGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2932..3672
                     /locus_tag="JN692_001734"
     CDS             2932..3672
                     /locus_tag="JN692_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7264065.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3707..4228
                     /gene="ruvC"
                     /locus_tag="JN692_001735"
     CDS             3707..4228
                     /gene="ruvC"
                     /locus_tag="JN692_001735"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7264066.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQAGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4230..4832)
                     /locus_tag="JN692_001736"
     CDS             complement(4230..4832)
                     /locus_tag="JN692_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HAY7264067.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSLQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4903..4968
                     /locus_tag="JN692_001737"
     CDS             4903..4968
                     /locus_tag="JN692_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264068.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5107..5718
                     /gene="ruvA"
                     /locus_tag="JN692_001738"
     CDS             5107..5718
                     /gene="ruvA"
                     /locus_tag="JN692_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7264069.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            5727..6737
                     /gene="ruvB"
                     /locus_tag="JN692_001739"
     CDS             5727..6737
                     /gene="ruvB"
                     /locus_tag="JN692_001739"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7264070.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6884..7669)
                     /gene="znuB"
                     /locus_tag="JN692_001740"
     CDS             complement(6884..7669)
                     /gene="znuB"
                     /locus_tag="JN692_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7264071.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7666..8421)
                     /gene="znuC"
                     /locus_tag="JN692_001741"
     CDS             complement(7666..8421)
                     /gene="znuC"
                     /locus_tag="JN692_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7264072.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8500..9432
                     /gene="znuA"
                     /locus_tag="JN692_001742"
     CDS             8500..9432
                     /gene="znuA"
                     /locus_tag="JN692_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7264073.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9448..10770
                     /gene="mepM"
                     /locus_tag="JN692_001743"
     CDS             9448..10770
                     /gene="mepM"
                     /locus_tag="JN692_001743"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7264074.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10890..11861
                     /gene="lpxM"
                     /locus_tag="JN692_001744"
                     /gene_synonym="msbB"
     CDS             10890..11861
                     /gene="lpxM"
                     /locus_tag="JN692_001744"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7264075.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11992..13434)
                     /gene="pyk"
                     /locus_tag="JN692_001745"
     CDS             complement(11992..13434)
                     /gene="pyk"
                     /locus_tag="JN692_001745"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7264076.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13562..14431)
                     /locus_tag="JN692_001746"
     CDS             complement(13562..14431)
                     /locus_tag="JN692_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7264077.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14768..16243
                     /gene="zwf"
                     /locus_tag="JN692_001747"
     CDS             14768..16243
                     /gene="zwf"
                     /locus_tag="JN692_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7264078.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16478..18289
                     /gene="edd"
                     /locus_tag="JN692_001748"
     CDS             16478..18289
                     /gene="edd"
                     /locus_tag="JN692_001748"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7264079.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18326..18967
                     /locus_tag="JN692_001749"
     CDS             18326..18967
                     /locus_tag="JN692_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7264080.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(19023..20201)
                     /gene="purT"
                     /locus_tag="JN692_001750"
     CDS             complement(19023..20201)
                     /gene="purT"
                     /locus_tag="JN692_001750"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7264081.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20336..20626
                     /gene="yebG"
                     /locus_tag="JN692_001751"
     CDS             20336..20626
                     /gene="yebG"
                     /locus_tag="JN692_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HAY7264082.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20656..21048
                     /gene="yebF"
                     /locus_tag="JN692_001752"
     CDS             20656..21048
                     /gene="yebF"
                     /locus_tag="JN692_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7264083.1"
                     /translation="MLSPESLCYGEKHEKRGAFLGRLLVSACASVFAANNETSKSVTF
                     PKCEGLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVP
                     LTVRGKSADIHYQVSVDCKAGMAEYQRR"
     gene            21375..22034
                     /locus_tag="JN692_001753"
     CDS             21375..22034
                     /locus_tag="JN692_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7264084.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQVIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22243..24303
                     /gene="ptrB"
                     /locus_tag="JN692_001754"
                     /pseudo
     CDS             22243..24303
                     /gene="ptrB"
                     /locus_tag="JN692_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(24300..24962)
                     /gene="exoX"
                     /locus_tag="JN692_001755"
     CDS             complement(24300..24962)
                     /gene="exoX"
                     /locus_tag="JN692_001755"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7264085.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24986..25642)
                     /locus_tag="JN692_001756"
     CDS             complement(24986..25642)
                     /locus_tag="JN692_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7264086.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIPGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(25744..25974)
                     /locus_tag="JN692_001757"
     CDS             complement(25744..25974)
                     /locus_tag="JN692_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7264087.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26113..26487
                     /gene="yobA"
                     /locus_tag="JN692_001758"
     CDS             26113..26487
                     /gene="yobA"
                     /locus_tag="JN692_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7264088.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26491..27363
                     /gene="copD"
                     /locus_tag="JN692_001759"
     CDS             26491..27363
                     /gene="copD"
                     /locus_tag="JN692_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HAY7264089.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLMIRRLLTKRSL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            27376..27717
                     /locus_tag="JN692_001760"
     CDS             27376..27717
                     /locus_tag="JN692_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7264090.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28110..>28471)
                     /locus_tag="JN692_001761"
     CDS             complement(28110..>28471)
                     /locus_tag="JN692_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7264091.1"
                     /translation="IAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGIRKNSPDG
                     SIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQRLLGHTSE
                     KTTKMYLDEREKTYLLL"
BASE COUNT         7203 a         7023 c         7392 g         6853 t
ORIGIN      
        1 caccccatta taaggggtac gaataaagta acgggggcat caaccggctg ttttaatgct
       61 tcggcgtaat cggcagacca gccaactcgc accagaaaca cgggctgacc gctggcgcga
      121 aatttcgccg ccagcttccc ggcgcgatta actacctcat cggcagtatg tggaccaccg
      181 gcaaaaggta agatgccttc ttgtaaatcg atcaccacca acgcagtggt tttagcatta
      241 agttcaagca tgctgactcc tgaaaaaaca aaatcgtgcc tcacacctta atgcgattag
      301 ccgatggcgg aacgacaaat tttgttaatt tttatgagtt cgggcaacac ctttatagca
      361 aactggcgta gagtctgatt tcaggcccga tataaagtgg taacgaggcg ttccagcgcg
      421 ggccaggctc ttacggtgcg gcagaatttc cagtataata gccgcctttt ttcattcagt
      481 tgtgacatac agctaacgct gcgacttggt cacctgcgca gcagggggca tccgccggca
      541 gctaagttaa gggatatctc atgcgtacag aatattgtgg acagctccgt ttgtcccacg
      601 tggggcagca ggtgactctg tgtggttggg tcaaccgtcg tcgtgatctt ggtagcctga
      661 tcttcatcga tatgcgcgac cgcgaaggta tcgtgcaggt atttttcgat ccggatcgtg
      721 cggacgcgtt aaagctggcc tctgaactgc gtaatgagtt ctgcattcag gttacgggca
      781 ccgtacgtgc gcgtgacgaa aaaaatatta accgcgatat ggcgaccggc gaaattgaag
      841 tgctggcgtc ctcgctgact atcatcaacc gcgcagatgt tctgccgctt gactctaacc
      901 acgtcaacac cgaagaagcg cgtctgaaat accgctacct cgacctgcgt cgcccggaaa
      961 tggcgcagcg cctgaaaacc cgcgctaaaa tcaccagcct ggtgcgccgt tttatggatg
     1021 accacggctt cctcgacatc gaaactccga tgctgaccaa agccacgccg gaaggcgcgc
     1081 gtgactacct ggtgccttct cgtgtgcaca aaggtaaatt ctacgcactg ccgcaatccc
     1141 cacagttgtt caaacagctg ctgatgatgt ccggttttga ccgttactat cagatcgtta
     1201 aatgcttccg tgacgaagac ctgcgcgctg atcgtcagcc tgaatttact cagatcgatg
     1261 tggaaacttc tttcatgacc gcgccgcaag tgcgtgaagt gatggaagcg ctggtgcgtc
     1321 atttgtggct ggaagtgaag ggcgtggatc tgggcgattt cccggtaatg acctttgcgg
     1381 aagcagaacg ccgttatggt tctgataaac cggatctgcg taacccgatg gaactgaccg
     1441 acgttgccga tctgctgaaa tctgttgagt ttgcagtatt tgcggggccg gcgaacgatc
     1501 cgaaaggccg tgttgccgct ctacgtgttc cgggtggcgc atcgctgacc cgtaagcaga
     1561 tcgacgaata cggtaacttc gttaaaatct acggcgcgaa aggtctggct tacatcaaag
     1621 ttaacgaacg cgcgaaaggt ctggaaggta tcaacagccc ggtagcgaag ttccttaatg
     1681 cagaaatcat cgaagccatc ctggatcgta ctgccgcgca agatggcgat atgattttct
     1741 tcggtgccga caacaagaaa attgttgccg acgggatggg cgcactgcgc ctgaaagtgg
     1801 gtaaagacct tggtctgacc gacgaaagca aatgggcacc gctgtgggtt atcgacttcc
     1861 cgatgtttga agacgacggt gaaggcggcc tgacggcaat gcaccatccg ttcacctcac
     1921 cgaaagacat gactgctgca gaactgaaag ctgcaccgga aaatgcggtg gcgaacgctt
     1981 acgatatggt catcaatggt tacgaagtgg gcggtggttc agtacgtatc cataatggtg
     2041 atatgcagca gacggtgttt ggtattctgg gtatcaacga agaggaacag cgcgagaaat
     2101 tcggcttcct gctcgacgcc ctgaaatacg gtactccgcc gcacgcaggt ctggcattcg
     2161 gtcttgaccg tctgaccatg ctgctgactg gcaccgacaa tatccgtgac gttatcgcct
     2221 tcccgaaaac cacggcggca gcgtgtctga tgactgaagc accgagcttt gctaacccga
     2281 ctgcactggc tgagctgagc attcaggttg tgaagaaggc tgagaataac tgatatgact
     2341 caaatacacg aaatcattcg cgttgcatca aggcggcaac tgagtgaact cccatgagca
     2401 tagataacta tgtgaatggg atgagcgaag gcagtcaacg aagaggcagc gtgaaggata
     2461 aagtgtataa gcgtcccgtt tcgatcttag tggtcatcta tgcacaagat acgaaacggg
     2521 tgctgatgtt gcagcggcgt gacgatcccg atttctggca gtcggtaacc ggcagcgtgg
     2581 aagagggtga aaccgcgccg caagctgcca tgcgcgaagt aaaggaagag gtcaccattg
     2641 atgttgtcgc tgaacaactg accttaattg actgtcagcg cacggtagag tttgaaattt
     2701 tttcacattt acgtcatcgc tatgcgcagg gcgtgacgcg taatacggaa tcatggttct
     2761 gtcttgcgct tccgcacgag cggcagatcg ttttcactga acatctggct tacaagtggc
     2821 ttgatgcgcc tgcggcggcg gcgctcacta agtcctggag caaccggcag gcgattgaac
     2881 agtttgtaat taacgctgcc tgaacaggca gggctatctg gagatatttt tatggcaggt
     2941 catagtaaat gggccaacac cagacatcgt aaagctgcgc aggatgctaa gcgcggtaaa
     3001 atcttcacta aaatcattcg tgagctggta accgcggcta agctgggcgg tggcgatccg
     3061 gacgctaacc cgcgtctgcg tgcggcggtg gataaagcac tgtctaacaa catgacccgt
     3121 gacacactga accgcgcaat tgcacgtggt gtgggcggtg atgatgatgc aaacatggaa
     3181 accatcatct acgaaggtta cggtcctggc ggcacggcaa tcatgattga atgtctgtct
     3241 gacaaccgca accgtaccgt tgctgaagtg cgtcacgcat tcagcaaatg tggcggtaac
     3301 ctcggcactg acggttccgt agcttatctg ttcagcaaaa aaggcgtgat ctccttcgag
     3361 aaaggcgacg aagacaccat catggaagca gctctggaag caggtgctga agacgttgtg
     3421 acatatgatg acggcgcgat tgatgtctac actgcatggg aagaaatggg taaagtgcgc
     3481 gacgctctgg aagcggcagg tctgaaagca gacagcgcgg aagtttccat gatcccgtcc
     3541 accaaagctg atatggatgc agaaaccgcg ccgaaactga tgcgtctgat cgatatgctg
     3601 gaagattgcg acgacgtgca ggaagtttac cataacggtg aaatctctga tgaggttgca
     3661 gcgactctct gatgatgcct gctaaacagc aaaacggaga cgcgtgatgg ctattattct
     3721 cggcattgac ccgggttcgc gcgtgaccgg ctacggcgtc atccgccagg caggtaggca
     3781 actgtcctac ctgggtagcg gatgcatacg caccaaagtg gatgatttac cgtctcgtct
     3841 gaagctcatc tatgcgggcg tgacggaaat catcacccag ttccagcctg attatttcgc
     3901 cattgaacaa gtctttatgg cgaagaacgc tgactcagcc ctgaaactgg gccaggcgcg
     3961 cggcgtggcg attgttgcgg cggtgaatca ggaattgccg gtatttgaat acgcggcacg
     4021 tcaggtaaag caaacggtgg taggtatggg tagtgccgaa aaaagccagg tgcagcatat
     4081 ggtccgcacc ttgctgaaac tgcccgctaa tccacaggcg gatgccgccg atgcgctggc
     4141 gattgccatc acccactgcc acgttagtca gaatgcgatg cagatgagcg aatcgcggct
     4201 gaacctggcg agagggcgac tgcgttaagt tataccgcct cggtcagttc cggctgaggc
     4261 gtttcaactc cctccgcgtg aatcaacacc aaacccggat gatgccacaa actggctggc
     4321 gtgacggttt tacgctccca cggaatagaa cctaagaacc agaatttcca gaaagtgagt
     4381 tttgcattcg gattgctatt taacagttgt ccaaacgtct ctatttcacc caccggaata
     4441 catagcgcct ctttataaat atcaaagaca aattttgaac agaactggag cgaagattcg
     4501 tatttaaaac ccgtgtggta aagtttgcgt agccgggagg gaacctgttc aacaattcgt
     4561 tgtttttgtt gttctgtcag cccagcgtct aatcgcttta tagcatagcg ttgattagac
     4621 gaacgtttaa taaaacgaga tagcgtggtg atggttgaaa ggggaacccg gctttctgca
     4681 accaggaagt cttcaccgtt atgaccgata atgatcccga cgtgattact ccagcaattt
     4741 gatgcagctg atatttgacc aaataaggca gcacctatac atgtaaagac gatatctcca
     4801 atttcatatt cagcagggta attaatattc acgttgtcat tccattgaaa tagatacgaa
     4861 tatacgtaat agcataaaaa aattattttt tcaggagtcg caatgtatat ttttattacg
     4921 catttcttca ctgaatatgt aatattcaaa tatttgcttc caatataacc tgttgaataa
     4981 attatactgt gccatttttc agatcatcgg gacacctcgc aagttttctt catccttcgc
     5041 tggatatcta tccagcattt ttttatcata cagcattatc tttgattcat tacgcaggag
     5101 cgtcatgtga taggcagact cagaggcatc atcattgaaa aacaaccccc gctggtgtta
     5161 attgaagtgg gcggcgtagg ctatgaagtg catatgccga tgacctgttt ttatgaactc
     5221 cctgaagcgg gtcaggaagc gatcgttttc acccactttg tggtgcgtga agacgcgcaa
     5281 ctgctgtacg gttttaacaa taaacaagaa cgcacgttgt tcaaagagtt gatcaaaacc
     5341 aacggcgtcg gcccgaagtt ggccctggcg atcctctccg gaatgtcagc gcagcagttc
     5401 gttaatgccg ttgagcgtga agaagtgggg gcactggtga aactgccggg tattggcaaa
     5461 aaaaccgccg aacgcttgat tgttgaaatg aaagaccgat ttaaaggttt gcatggcgat
     5521 ctctttacgc cagccgccga cctggtactc acgtctcctg ccagcccggc gaccgacgat
     5581 gctgaacaag aagcagttgc cgcgctggtg gcgctaggct ataaaccaca agaagcaagc
     5641 cgcatggtga gcaaaatcgc tcgccctgac gccagcagtg aaacattaat tcgcgaagcc
     5701 ctacgcgccg cgttatgagg taaaggatga ttgaagcaga ccgtctgatt tctgccggta
     5761 ccactttgcc ggaagatgta gcagatcgcg ccattcgccc taaattactg gaagagtatg
     5821 ttggtcagcc gcaggttcgt tcacagatgg agattttcat caaagcagcg aaactgcgcg
     5881 gcgatgccct cgatcatttg ttgatttttg gtcctccggg gttgggtaaa actacgcttg
     5941 ccaacattgt cgccaatgaa atgggcgtta atttacgcac gacttctggt ccggtgctgg
     6001 aaaaggcggg cgatttggct gcgatgctca ctaaccttga accgcatgac gtgctgttta
     6061 ttgatgagat ccaccgttta tcgccagttg ttgaagaagt gctgtacccg gcaatggaag
     6121 actaccaact ggatatcatg attggtgaag gtccggcggc acgctccatt aaaattgatt
     6181 tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg ttcgctgaca tcaccgttgc
     6241 gcgaccgttt tggtattgtg caacgtctgg agttttatca ggtgccggat ctgcaatata
     6301 tcgtcagtcg cagtgcacgc tttatggggc ttgagatgag tgatgacggt gcgctggaag
     6361 tagctcgtcg cgctcgcggt acgccacgta ttgccaaccg tctgctgcgt cgagtgcgtg
     6421 atttcgccga agtgaagcac gatggcacca tctcggcaga tatcgctgct caggcgctgg
     6481 atatgttgaa tgtcgatgct gaaggtttcg attatatgga ccgcaaattg ttgctggcgg
     6541 taatcgataa gttctttggt gggccggtag gtctggataa cctggcggca gccattggcg
     6601 aagaacgtga aaccattgaa gatgtactgg aaccttattt gattcagcaa ggctttttgc
     6661 agcgtacacc gcgcgggcgt atggcgacga cgcgggcgtg gaatcacttt ggcataacgc
     6721 cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc atccgacact ggcagtgtgt
     6781 ctgatgcgac actgttcgcg tcttatcagg ccagccagag atagcgaacc gtaggtcgga
     6841 taaggcgctc gcgccgcatc cgacaaatgt gttcagcgat agattagctg gcctgctttt
     6901 tcatcatact gataataaat agcagtgccg cacatagcac caccgacgga cccgccggcg
     6961 tatcgtaaaa cgcggaaaag gttaaaccgc cagttactgc caccatcccc accaaaacag
     7021 cgacaccagc catctgttcc ggcgtacggg caaagcgacg cgcagtagct gcaggaataa
     7081 tcagcaacga agtaataatc aacgcaccga caaatttcat cgctacacca atcgtcaatg
     7141 ccgtcaccag catcaacaac aatttcactt gctgtaattt cacaccatca acaaacgcca
     7201 gatccgggct aatcgtcatc gacagcaaat tgcgccattg ccagaacaaa atagccacca
     7261 cgatgaccac gccaatcgca atagagatga gatcttctgg cgtcactgcc agtaaatcac
     7321 cgaacaggta agccatcaaa tcaacacgaa tgttagacat cagactaacg accactaggc
     7381 ccagcgacag ggcactgtgc gccataatcc ctaataacgt gtcgatcgcc agctgtggac
     7441 gcttctccag ccataccaga ccgcccgcca gcagcagcgt aacggcaatc accgcataga
     7501 atggattcac gtccagcaac aaaccaaacg cgacaccaag taatgaggca tgagccagcg
     7561 tatcaccgaa ataagacata cgacgccaga ctacaaacga acccagcgga cccgcggcac
     7621 aggcgagcat gatcccggct aaccaaccgg gaaataataa ttcaatcatg agcgatcatt
     7681 tccccgacgc aaaacaattc gtccctgtaa atcgtgacga tgattatgat gatggcgata
     7741 gatacccagt tgttcagcac cacgaggacc aaacattgaa ataaactccg gatgcaggga
     7801 aacaacttcc ggtgtgccgg aacaacaaat gtggtgattc aggcacagca cttcatcggt
     7861 ttttgccatc accagatgca gatcgtgaga aaccattaaa acgccacaat ccagttcacg
     7921 acgcaactgg tcaataaggt catataacgc cacttggcca tttacatcca cgccttgagt
     7981 gggttcatcc agcactaata attgcgggca atttaacaat gctcgcgcta atagtacacg
     8041 ctgcgtttcg ccaccagaga gcttttgcat cggtgcgtta atcaggtgcc cggcctggac
     8101 acgtttcagt gcaggcaaaa tatcttcttt atgtgtacca gggcgtaagc gtaaaaaacg
     8161 gtttacggtc agtggcaacg tggtgtcgag atacagcttc tgcggtacat agccgatgcg
     8221 cagttttccg ttgcgcttga taaccccttc atcgggtgtt accagcccga gcactacccg
     8281 taccagtgtc gacttacctg cgccatttgg cccaagtaaa gtcaaaattt ttccaggttt
     8341 aagttccagc gacacatcag agaggacgcg gcgttggcca aaagaaaccg agacattttc
     8401 cagggaaacc agacttgtca tgttaatttt agtcttgcag tagtcatgaa atgttataat
     8461 atcacacttc tcatattcat tacgatgatt ggtcgcatta tgttacataa aaaaacgctt
     8521 cttttcgcag cattatccgc cgctctctgg ggaggtgcaa cacaggccgc agatgccgcc
     8581 gttgtcgctt cgcttaaacc cgttgggttc atcgcttctg ccattgctga tggtgtaaca
     8641 gaaacggagg ttttactgcc tgacggcgct tcagaacatg attattcact gcgtccatcg
     8701 gatgtaaaac gcttacagaa cgcggactta gtcgtttggg ttggcccgga gatggaagcg
     8761 tttatgcaaa aaccggtaag caaattacca gaagcgaagc aggtaacgat tgcgcagctt
     8821 gaagatgtga aaccgctact gatgaaaagt attcacggcg atgatgatga tcacgaccac
     8881 gcggaaaaaa gtgacgaaga tcaccatcac ggcgatttca acatgcatct ttggctttcc
     8941 ccagagatag cgcgggctac agcggttgca atccatggaa aattagtgga acttatgccg
     9001 caaagtcgag ccaaacttga cgccaacctg aaggattttg aggcacaatt ggcctcaacc
     9061 gaaacgcagg ttggtaacga gctcgcgccg ctcaagggga aaggttattt cgtttttcac
     9121 gatgcttacg gctatttcga aaaacagttc ggactgacac cgcttggtca ttttaccgtt
     9181 aaccctgaga ttcaacctgg cgcgcagcgt ttacatgaaa taagaacaca gttggttgag
     9241 caaaaagcaa cctgcgtttt tgctgagcca cagttcaggc cagcggtcgt tgagagcgtc
     9301 gcacgaggga catccgttcg tatgggaacg ttggatcccc tggggacgaa tatcaaactg
     9361 ggtaaaacaa gctattcaga attcctgagt caattagcca accagtatgc gagctgcctg
     9421 aaaggagatt aatgaggaag tgattacgtg caacagatag cccgctctgt cgccctggcg
     9481 tttaataatt taccgcgacc acaccgcgtt atgttggggt cgctcaccgt tcttactctg
     9541 gccgtcgctg tctggcggcc ctatgtttat caccgtgatg ccacgccaat tgtcaaaacc
     9601 attgagctgg aacagaacga aattcgttcg ctcttacctg aagccagtga gccgattgat
     9661 caagctgcac aagaagatga agccattccc caggacgaac tggatgacaa aatcgccggt
     9721 gaagcgggcg tgcatgaata tgttgtttcc actggcgata cgctaagcag cattctcaat
     9781 cagtatggta ttgatatggg tgatatcacc caactggctg ctgccgacaa agaattgcgt
     9841 aacctgaaaa tcggtcaaca actctcctgg acattaaccg cggacggcga actgcagcgc
     9901 ctcacctggg aagtgtctcg tcgtgaaacc cgaacctatg accgtactgc cgctaacggt
     9961 tttaaaatga ccagcgaaat gcagcaagga gagtgggtta acaatctgct gaaaggtacc
    10021 gtcggaggga gctttgttgc cagcgccaga aacgccggtt taaccagcgc cgaagtgagc
    10081 gcagtgatta aagccatgca gtggcaaatg gatttccgca aactgaaaaa aggcgatgaa
    10141 tttgcggtat taatgtctcg agaaatgctt gatggtaaac gtgagcaaag ccagctgctg
    10201 ggcgtacgtt tgcgttcaga aggtaaagat tattacgcaa tccgcgctga ggatggcaaa
    10261 ttctacgatc gtaacggtac tggtctggcg aaaggattct tgcgattccc gacggcgaaa
    10321 cagttccgta tctcgtctaa ctttaacccg cgtcgaacta atccggtgac aggtcgcgtt
    10381 gcaccacaca gaggtgttga tttcgccatg ccacaaggta cgccagtgct ttcagtgggt
    10441 gacggtgaag tggtggttgc caaacgcagt ggcgcagcag gttattatgt ggctattcgt
    10501 catggtcgca gctacaccac gcgttatatg cacttgcgca agattctggt gaaaccggga
    10561 cagaaggtga aacgtggcga ccgtatcgcg ctttccggta ataccggacg ttcaactggg
    10621 ccgcatctgc actatgaagt atggataaac cagcaggccg taaacccgct gacggcaaaa
    10681 ctgccgcgta ccgaagggct gaccggctcc gatcgtcgcg aattcctggc gcaggccaaa
    10741 gagattgtgc cgcagctacg gtttgattaa ttaacatcca ttcgcagccg gtacgcagtc
    10801 agtaccggct ttttttattt ggtgcggggc aagttgcgcc gctacactat caccagattg
    10861 atttttgcct tatccgaaac tggaaaagca tggaaacgaa aaaaaataat agcgaataca
    10921 ttcctgagtt tgataaatcc tttcgccacc cgcgctactg gggagcatgg ctgggtgtag
    10981 cagcgatggc gggtatcgct ttaacgccgc caaagttccg tgatcccatt ctggcacggc
    11041 tgggacgttt tgccggacga ctgggaaaaa gctcacgccg tcgtgcgtta atcaatctgt
    11101 cgctctgctt tccagaacgt agtgaagctg aacgcgaagc gattgttgat gagatgtttg
    11161 ccaccgcgcc gcaagcgatg gcaatgatgg ctgagttggc aatacgcggg ccggagaaaa
    11221 ttcagccgcg cgttggctgg caagggctgg agatcatcga agagatgcgg cgtaataacg
    11281 agaaagttat ctttctggtg ccgcacggtt gggccgtcga tattcctgcc atgctgatgg
    11341 cctcgcaagg gcagaaaatg gcagcgatgt tccataatca gggcaacccg gtttttgatt
    11401 atgtctggaa cacggtgcgt cgtcgctttg gcggtcgtct gcatgcgaga aatgacggta
    11461 ttaaaccatt catccagtcg gtacgtcagg ggtactgggg atattattta cccgatcagg
    11521 atcatggccc agagcacagc gaatttgtgg atttctttgc cacctataaa gcgacgttgc
    11581 ccgcgattgg tcgtttgatg aaagtatgcc gtgcgcgcgt tgtaccgctg tttccgattt
    11641 atgatggcaa gacgcatcgt ctgacgattc aggtgcgccc accgatggat gatctgttag
    11701 aggcggatga tcatacgatt gagcggcgga tgaatgaaga agtcgagatt tttgttggtc
    11761 cgcgaccaga acaatacacc tggatactaa aattgctgaa aactcgcaaa ccgggcgaaa
    11821 tccagccgta taagcgcaaa gatctttatc ccatcaaata aaaaagcctc tcgcgaggag
    11881 aggccttcgc ctgatgataa gttcaagttt gcttcagaat attcgaaatc tgttgaacta
    11941 tcattgaact ataggccgga tgtggcgttt tcgccgcatc cggcaacgta cttactctac
    12001 cgttaaaata cgcgtggtat tagtagaacc cacggtactc atcacgtcgc cctgggtgac
    12061 aatcaccagg tcaccagaca tcaagtaacc tttatcgcgc agcagattaa ccgcttcgct
    12121 ggcagctgct acgccgtcat tagcgctatc aaagtgcacc ggcgtaacgc cacgatagag
    12181 agcagtcagg ttcagcgtac gttcatggcg cgacatggcg aaaattggta gaccagagct
    12241 gatacgggag gtcatcagcg cggtacgacc cgattcggtc atggtaatga tcgccgtaac
    12301 gcccttcagg tggttagctg cgtacattgc cgacatggca atagcttctt ccacattgtc
    12361 gaactgaacg tccagacggt gtttagaaac gttgatgctc gggatttttt ccgcacccag
    12421 gcaaacgcgc gccatggctg caacagtttc tgacggatac tgcccagcgg cagtttctgc
    12481 agacagcatc acagcgtcag taccatccag aacggcgttt gctacgtcca tgacttctgc
    12541 acgcgtcggc atcgggttag taatcattga ctccatcatc tgggtcgccg tgattaccgc
    12601 tcggtttagc tgacgcgcac gacggatcaa cgctttctga atgccgacca gttccgggtc
    12661 gccaatttcc acaccgaggt cgccacgtgc aaccattacc acgtcagagg cgaggatgat
    12721 gtcatccatt gcatcctggc tgcaaacggc ttccgcacgt tcaaccttgg caacaatttt
    12781 cgcatcacat cctgcatcgc gtgccaggcg acgggcatag ttcagatctt cgccacagcg
    12841 tgggaaggag acagccaggt aatctacgcc aatcaacgcc gcagtcttaa tgtctgcttt
    12901 gtctttttcg gtcagcgctt cagccgacaa accgccgcca agtttgttga tacctttatt
    12961 gttggagagg ggaccaccga cggtgacttc ggtgaacact ttcatgccct gaacttccag
    13021 tacttttaac tggacgcgac catcgtccag cagcaggatg tcaccaggca cgacgtcagc
    13081 aggcaggcct ttgtagtcga taccgacttt ttctttgtcg ccttcacctt tacctaggtt
    13141 ggcgtcgagc aggaatttat ccccaatatt gaggaaaact ttgccttctt taaaggtgga
    13201 tacacggatt ttgggcccct ggaggtcacc cagaatagcc acatgacgcc ccagttttgc
    13261 ggcaatctca cgaactttat ccgcgcgcat tttgtgatct tcaggcgagc cgtgagaaaa
    13321 gttcatacgt acaacgttgg cacccgccgc gataactttt tcaagattat tatcgcgatc
    13381 tgttgctggg cctaacgtgg taacgatttt tgttctgcga agccttttgg acatgtaata
    13441 ctccgttgac tgaaacaacc aggtgttgct tgaacatgaa atccgaatga aataacgccg
    13501 cgatggtgat aaattaatct cattcggatg tcgtataaga ttaggacagt gacagtcgct
    13561 tttagcgatc gtcacttaaa ttaagtaact gcttatcaaa acgcgattcc ttcagcgctt
    13621 ccttgacccg cttcaagtta tctctgaatt ttgcaccgcg tcgcaaagta aatcctgtcg
    13681 ccagcacatc tatcacggtc agctgtgcaa gtcgagaaac catgggcata taaatgtcag
    13741 tatcttccgg tacgtcgagg gtaattgcca gcgttgcttc ccgggcgagc ggggtacccg
    13801 cagaggtgag ggcaatcacc atggcgtcgt tttcgcgtgc cagctgcgcc agctcgacca
    13861 gattttttgt tcttcctgtg tgagaaatca gcaccaccac gtctccgtcg ctacaattca
    13921 tacaactcat gcgttgcagc acgatatcat cggagtacac caccggaaca ttaaaacgaa
    13981 agaacttatt catcgcatcg tgggcaacgg cggcagaaga gcctaatccg aaaaaggcga
    14041 ttttttttgc ctgagtgagc aagtcgacgg cgcggttgat ggcagattta tccagtgaat
    14101 gacggacatg atcgagcgtt gccattgcgg actcaaatat tttccccgtg tatgattcaa
    14161 cgctgtcatc ttcattgacg ttgcgattaa catagggagt gccattcgcc agactttgtg
    14221 ccagatgaag tttaaaatca ggaaaaccgc gcgtgtccat gctgcgacag aaacgattca
    14281 ccgtcggttc gctaacattg gcttccagcg ccagagcagc aatactcgaa tggatcgcgt
    14341 tatcgggcga agccagaatg acctcggcaa ctttgcgctc tgatttgctc aaatgttcca
    14401 gctgagactg gattttttcc agcatattca tgatgtaaag agactcacgg gtaatgacga
    14461 tttccgcact gaaagaaatc gaaatgcagt tttgtcagat attacgcctg tgtgccgtgt
    14521 taatgacaaa agcagataaa aaagttgtta tttttttcat aacatgatca gtgtcagatt
    14581 tttaccgaat ggaaaacgat gattttttta tcagttttgc cgcactttgc gcgcttttcc
    14641 cgtaatcgca cgggtggata agcgtttaca gttttcgcaa gctcgtaaaa gcagtacagt
    14701 gcaccgtaag aaaattacaa gtataccctg gcttaagtac cgggttagtt aacttaagga
    14761 gaatgacatg gcggtaacgc aaacagccca ggcctgtgac ctggtcattt tcggcgcgaa
    14821 aggcgacctt gcgcgtcgta aattgctgcc ttccctgtat caactggaaa aagccggtca
    14881 gctcaacccg gacacccgga ttatcggcgt agggcgtgct gactgggata aagcggcata
    14941 taccaaagtt gtccgcgagg cgctcgaaac tttcatgaaa gaaaccattg atgaaggttt
    15001 atgggacacc ctgagtgcac gtctggattt ttgtaatctc gatgtcaatg acactgctgc
    15061 attcaaccgt ctcggcgcga tgctggatca aaaaaatcgt atcaccatta actactttgc
    15121 catgccgccc agcacttttg gcgcaatttg caaagggctt ggcgaggcaa aactgaatgc
    15181 taaaccggca cgcgtagtca tggagaaacc gctggggacg tcgctggcga cctcgcagga
    15241 aatcaatgat caggttggcg aatacttcga ggagtgccag gtttaccgta tcgaccacta
    15301 tcttggtaaa gaaacggtgc tgaacctgtt ggcgctgcgt tttgctaact ccctgtttgt
    15361 gaataactgg gacaatcgca ccattgatca tgttgagatt accgtggcag aagaagtggg
    15421 gatcgaaggg cgctggggct attttgataa agccggtcag atgcgtgaca tgattcagaa
    15481 ccacctgcta caaattctct gcatgattgc gatgtctccg ccatctgacc tgagcgcaga
    15541 cagcatccgc gatgaaaaag tgaaagtact gaagtccctg cgccgcatcg accgctccaa
    15601 cgtacgcgaa aaaaccgtac gcgggcaata tactgcgggc tttgcccagg gcaaaaaagt
    15661 gccgggatat ctggaagaag agggcgcgaa caaaagcagc aatacagaaa ccttcgtggc
    15721 gatccgcgtc gacattgata actggcgctg ggccggtgtg ccattctacc tgcgtactgg
    15781 taaacgtctg ccgaccaaat gttctgaagt cgtggtctat ttcaaaacac ctgaactgaa
    15841 tctgtttaaa gagtcgtggc aggatctgcc gcagaataaa ctgactatcc gtctacaacc
    15901 tgatgaaggc gtggatatcc aggtactgaa taaagttcct ggccttgacc acaaacataa
    15961 cctgcaaatc accaagctgg atctgagcta ttcagaaacc tttaatcaga cgcatctggc
    16021 ggatgcctat gaacgtttgc tgctggaaac catgcgtggt attcaggcac tgtttgtacg
    16081 tcgcgacgaa gtggaagaag cctggaaatg ggtagactcc attactgagg cgtgggcgat
    16141 ggacaatgat gcgccgaaac cgtatcaggc cggaacctgg ggacccgttg cctcggtggc
    16201 gatgattacc cgtgatggtc gttcctggaa tgagtttgag taatatctgt gcttatcctt
    16261 tatggttatt ttaccggtaa catgatcttg cacagattgt agaacaattt ttacactttc
    16321 aggcctcgtg cggattcacc cacgaggctt tttttattac actgactgaa acgtttttgc
    16381 cctatgagct ccggttacag gcgtttcact cataaatcct ctgaatgaaa cgcgttgtga
    16441 atcatcctgc tctgacaact caatttcagg agcctttatg aatccacaat tgttacgcgt
    16501 aacaaatcga atcattgaac gttcgcgcga gactcgctct gcttatctcg cccggataga
    16561 acaagcgaaa acgtcgaccg ttcatcgttc gcagttggca tgcggtaacc tggcacacgg
    16621 tttcgctgcc tgccagccag aagacaaagc ctctttgaaa agcatgttgc gtaacaatat
    16681 cgccatcatc acctcctata acgacatgct ctccgcgcac cagccttatg aacactatcc
    16741 agaaatcatt cgtaaagccc tgcatgaagc gaatgcggtt ggtcaggttg cgggcggtgt
    16801 tccggcgatg tgtgatggtg tcacccaggg gcaggatgga atggaattgt cgctgctaag
    16861 ccgcgaagtg atagcgatgt ctgcggcggt ggggctgtcc cataacatgt ttgatggtgc
    16921 tctgttcctc ggtgtgtgcg acaagattgt cccgggtctg acgatggcag ccctgtcgtt
    16981 tggtcatttg cctgcggtgt ttgtgccgtc tggaccgatg gcaagcggtt tgccaaataa
    17041 agaaaaagtg cgtattcgcc agctttatgc cgaaggtaaa gtggaccgca tggccttact
    17101 ggagtcagaa gccgcgtctt accatgcgcc gggaacatgt actttctacg gtactgccaa
    17161 cactaaccag atggtggtgg aatttatggg gatgcagttg ccaggctctt cttttgttca
    17221 tccggattct ccgctgcgcg atgctttgac cgctgccgct gcgcgtcagg ttacacgcat
    17281 gaccggtaat ggtaatgaat ggatgccgat cggtaagatg atcgatgaga aagtggtggt
    17341 gaacggtatc gttgcactgc tggcgaccgg tggttccact aaccacacca tgcacctggt
    17401 ggcgatggcg cgcgcggccg gtattcagat taactgggat gacttctctg acctttctga
    17461 tgttgtaccg ctgatggcac gtctctaccc gaacggtccg gccgatatta accacttcca
    17521 ggcggcaggt ggcgtaccgg ttctggtgcg tgaactgctc aaagctggcc tgctgcatga
    17581 agatgtcaat acggtggcag gctttggtct gtctcgttat acccttgaac catggctgaa
    17641 taatggtgaa ctggactggc gggaaggggc ggaaaaatca ctcgacagca atgtgatcgc
    17701 ttccttcgaa caacctttct ctcatcatgg tgggacaaaa gtgttaagcg gtaacctggg
    17761 ccgtgcggtt atgaaaacct ctgccgtgcc ggttgagaac caggtgattg aagcgccagc
    17821 ggttgttttt gaaagccagc atgacgttat gccggccttt gaagcgggtt tgctggaccg
    17881 cgattgtgtc gttgttgtcc gtcatcaggg gccaaaagcg aacggaatgc cagaattaca
    17941 taaactcatg ccgccacttg gtgtattatt ggaccggtgt ttcaaaattg cgttagttac
    18001 cgatggacga ctctccggcg cttcaggtaa agtgccgtca gctatccacg taacaccaga
    18061 agcctacgat ggcgggctgc tggcaaaagt gcgcgacggg gacatcattc gtgtgaatgg
    18121 acagacaggc gaactgacgc tgctggtaga cgaagcggaa ctggctgctc gcgaaccgca
    18181 cattcctgac ctgagcgcgt cacgcgtggg aacaggacgt gaattattca gcgccttgcg
    18241 tgaaaaactg tccggtgccg aacagggcgc aacctgtatc actttttaag acgacaaatt
    18301 tgtaatcagg cgagagaaaa ctctgatgaa aaactggaaa acaagtgcag aatcaatcct
    18361 gaccaccggc ccggttgtac cggttatcgt ggtaaaaaaa ctggaacacg cggtgccgat
    18421 ggcaaaagcg ttggttgctg gtggggtgcg cgttctggaa gtgactctgc gtaccgagtg
    18481 tgcagttgac gctatccgtg ctatcgccaa agaagtgcct gaagcgattg tgggtgccgg
    18541 tacggtgctg aatccacagc agctggcaga agtcactgaa gcgggtgcac agttcgcaat
    18601 tagcccgggt ctgaccgagc cgctgctgaa agctgctacc gaagggacta ttcctctgat
    18661 tccggggatc agcactgttt ccgaactgat gctgggtatg gactacggtt tgaaagagtt
    18721 caaattcttc ccggctgaag ctaacggcgg cgtgaaagcc ctgcaggcga tcgcgggtcc
    18781 gttctcccag gtccgtttct gcccgacggg tggtatttct ccggctaact accgtgacta
    18841 cctggcactg aaaagcgtgc tgtgcatcgg tggttcctgg ctggttccgg cagatgcgct
    18901 ggaagcgggc gattacgacc gcattactaa gctggcgcgt gaagctgtag aaggcgctaa
    18961 gctgtaaaag tcaaaatgcc cgatctagga tcgggcattt ttgtagcgtt tttttgccgg
    19021 gtttaaccct gtacttttac ctgtccggcg gcgtgcttcg cgcgttcaat ggcgtcaaca
    19081 acactctctg aagtagccag taccaccccc agacgacggc tgccatcaat ttccggctta
    19141 ccaaataaac gaatctgcaa atctgcgcct acggcattct gcacattatc aaacgtgaca
    19201 ttctgactgg tcagttgtgg cagaataacg gcagaagctg caggaccata ctgacggatc
    19261 ccgccaacag gaagtccgag gaaggcacgt acatgcaggg caaactctga gagatcttga
    19321 gaaattaacg tcaccatccc ggtatcatgt ggacgagggg agacctcact gaaaatcacc
    19381 tcatcaccac agacaaacag ctcgacacca aacagaccat aaccgcccag tgccagcacc
    19441 actttacggg caatctcctg cgcacgttca agggcaagcg ggctcatttg ctgtggttgc
    19501 caggattcac ggtagtcgcc atcttcctgg cgatgaccta ctggtgcaca gaaatggacg
    19561 ccatccaccg cgctgacggt tagcagggta atttcgaagt caaacttaac gacgccttca
    19621 acaattacgc ggcccgctcc ggcgcgaccg ccttgctgag cgtattccca tgcctgagca
    19681 agttgctctg cagagcgaat aaacgtctgc cccttgccgg aagagctcat caccggtttt
    19741 acaatgcagg gatagccaat ggcggcaacc gcctcgcgga aaaggctttc gctatcggca
    19801 aaacgataag tggaagtggg cagctgcagc tcttctgccg ccaggcgacg gataccctcg
    19861 cgattcatcg ttaatttcgt tgcgcgagcg caggggacaa cattcagtcc ttcctcttca
    19921 agttggatca gcatatcggt ggcaatagct tcgatctccg gcacgatata atgtggtttt
    19981 tccagttcaa ccacacggcg taatgcatca ccatcaagca tattaatgac atgggagcga
    20041 tgcgcgacat gcatggctgg tgcgtcggca tagcgatcga cggcaatcac ctctacgccg
    20101 agacgctgac actcgattgc cacttcttta cccagttcac cggagcctaa taacatcacg
    20161 cgagttgctg ccggacgcag cgcagtgcct aataacgtca taacgaatat cccctgatta
    20221 attccgcgcg cagtataaac gaaaacgttt gcgtgtgtct ttattgctga tgctgatttc
    20281 tcaaccgaaa agaaatatac tgatataaaa tcacagttat tatgagaggc tgcaaatggc
    20341 ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt ttaccagcaa
    20401 aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg acacctggct
    20461 gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc tatggctggc
    20521 agaacaaaaa gatgtgctga gcaccattct gaaaaccggc aaattaccgt ccccacaggt
    20581 cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca acttgcgctc
    20641 ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa acatgaaaaa
    20701 agaggggcgt ttttagggcg gttgttggtt tctgcctgcg catcagtttt cgctgccaat
    20761 aatgaaacca gcaagtcggt cactttccca aagtgtgaag gtctggatgc tgccggaatt
    20821 gccgcgagcg taaaacgtga ttatcaacaa aatcgcgtgg cgcgctgggc agatgatcaa
    20881 aaaattgtcg gtcaggccga tcccgtggcc tgggtcagtt tgcaggacat tcagggtaaa
    20941 gatgataaat ggtcagtacc gctaaccgtg cgtggtaaaa gtgccgatat tcattaccag
    21001 gtcagcgtgg actgcaaagc gggaatggcg gaatatcagc ggcgttaatg gcttctgata
    21061 attcgattga ccgttggcgg atgcggcgcg agtgccttgt ccgccccaga acgcaccaca
    21121 atcgtaggcc tgagaagacg caacagcgtc gcatcgggca atcagcatca aacaatgcca
    21181 ctcaaaacga caaactactt tcacctaaaa tacccttatc acctgatata cagtaaatac
    21241 cttacctggc gcgaatttct ttgaataacg gcctaccgcc ccgcttattt gcgcatcttt
    21301 tgtctcgata tacattttct gacatcctca tccgctatgc tacaggcact aaaaatggtt
    21361 attgaggagc aaatatggct aactggttaa atcaactgca atccctgctt gggcaaagca
    21421 gttcttctac ctcctcgtct gcggatcagg gattgggcaa actgttagtg ccaggcgcat
    21481 taggcggact ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt cttacgaaat
    21541 atggcaccaa cgcgttactg gttggcggcg gagcggtagc gggtacggtg ctgtggaata
    21601 aatacaaaga taaaattcgc gcggcgcatc aggacgaacc gcagtttggc gcgcaaagta
    21661 cgccgctgga tgagcgtacg gaacgtttga tccttgcgct ggtctttgcc gctaaaagtg
    21721 atggtcatat tgatgccaat gaacgtgcgg caatcgacca gcaattgcgt gaagccggcg
    21781 tggaagagca ggggcgtgta ctcattgagc aggtaatcga acaaccgctg gatccacaac
    21841 gcctggctac cggggtccgc aatgaagaag aggcgctgga aatctatttc ctgagctgcg
    21901 cggctattga tattgaccat tttatggaac gcagttacct gaatgcactg ggtgacgcgc
    21961 tgaaaatccc ccaggatgtt cgtgaaggca ttgaacgcga tctcgaacag caaaaacgca
    22021 ctttggcgga ataattccgc tctctggata gcctgaagtt gactatttcg ggctatccag
    22081 aaccgcacag ccatttgaac aggctgattt tctctgtctg cataacattc gatatccgcg
    22141 ccattacctg tcatcatcta agcaatgacg accctgtttc gcttgcatcc ccggtgagtt
    22201 ttgccaccct tataagatgt ttcaaccaga aagaacaata acatgctacc aaaagccgcc
    22261 cgcattcccc acgccatgac gcttcatggc gatacgcgca tcgataatta ctactggctg
    22321 cgggacgata cgcgttctca gccagaagtc ctggactacc tgcaacaaga aaatagttac
    22381 ggtcatcggg tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa ggaaatcatc
    22441 gaccgcattc cgcaaagaga agtttctgcg ccctacataa aaaatggcta ccgctatcgg
    22501 catatttatg aaccaggctg tgaatatgct atctaccagc gtcaatcggc attcagcgaa
    22561 gagtgggatg agtgggaaac attgctcgat gccaacaagc gcgcagctca tagtgagttt
    22621 tattcgatgg gcggaatggc gattacgccc gataacacca ttatggcgct ggcagaagat
    22681 tttctttccc gacgccagta cggcattcgt tttcgtaatc tggaaacagg taactggtac
    22741 ccggaactgc tggataacgt tgaacccagc tttgtctggg caaatgactc ctggactttc
    22801 tactatgttc gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg tcacgccatc
    22861 ggtacgccag catcgcaaga tacactgatc tacgaagaaa aagacgatac ctattatgtc
    22921 agcctgcata aaacgacgtc gaagcactat gtcgtcattc atctggccag cgccaccacc
    22981 agtgaagttc gcctgctgga cgcggaaatg gccgatgccg agccgtttgt ttttctgccg
    23041 cgccgcaaag atcacgaata cagccttgat cactaccagc atcgttttta tctgcgttcc
    23101 aatcgccacg gcaaaaactt tggcttatac cgtacccgta tgcgtgatga gcaacagtgg
    23161 gaagagttaa ttccgccacg cgaaaacatc atgctggaag ggtttacgct gtttaccgac
    23221 tggctggtgg ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat taaccgcaag
    23281 acccgggaag tcattggcat tgcctttgat gatccggcct atgtgacctg gattgcctac
    23341 aatccagaac ctgaaaccgc gcgattgcgt tatggttatt cttccatgac cacaccagac
    23401 actttgtttg aactggatat ggataccggt gagcgtcgtg tattaaaaca aacagaagtg
    23461 cccggttttg atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc ccgtgatggc
    23521 gtcgaagttc cggtttcgct ggtctatcat cgcaaacatt ttcgcaaagg acacaacccg
    23581 ttgctggtgt atggctatgg ttcttacggc gcaagtattg atgccgattt cagttttagc
    23641 cgcttgagtt tgttagatcg tggctttgtc tacgccattg tccatgttcg cggcggtggt
    23701 gagctggggc aacaatggta cgaagacggt aaatttctga agaagaaaaa tacgtttaat
    23761 gattatcttg atgcctgcga tgcattgtta aaactgggct atggctctcc ttcgctctgt
    23821 tatgcgatgg gcggaagtgc ggggggcatg ttgatgggcg ttgcaattaa ttaacgcccg
    23881 gaattattcc acggcgttat cgcccaggta ccgtttgttg atgttgtaac aacgatgctt
    23941 gatgaatcaa ttcctcttac cactggtgag tttgaagagt gggggaatcc gcagggtccg
    24001 caatattacg agtacatgaa aagctacagc ccgtatgaca acgtcaccgc acaggcttat
    24061 ccgcatttac tggtaacgac cggtttacac gattctcagg tgcaatattg ggaaccggca
    24121 aaatgggtcg ctaaattgcg cgagctgaaa accgatgacc atcttttatt gctctgtacc
    24181 gacatggact caggccatgg cggtaaatct ggccgcttta aatcgtacga aggcgtagcg
    24241 atggaatatg cttttctggt cgcgctggcg cagggaacat tacccgctac gcctgcggat
    24301 taagtatttt ccagataatg tttcagtgtt aaacgcagct ccgggctcat gctgtccagg
    24361 ttattaaata accagcgcag atagcccgga tcgcgttcgg caacgtcgga aaccgctttg
    24421 ccacggtatt tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt gatatcggcc
    24481 atctgttctg ccgtccagcc ggaggtgttc ataatatcga taagcaacgc ggcggtgata
    24541 taacagtcat acaacgcgcg gtgatgatgc aggcccggcg gtgtctgtac attgagcttg
    24601 cgtgttttat ataacgccat attgctgtac ttgagccctg gccacaaacg acgggccagt
    24661 ttcatagtgc aaatccactc accgggcatc tcaggcagta cgcggcggtc aaagctggcg
    24721 ttgtgcgcga cataccattc actaccgtaa tagtgtggga tcacatcttc aatccacggt
    24781 ttatcggcga ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg actaatagga
    24841 cgatcggggc gcaccaggag gctcatgggg ttgacgattt ttccgtcaat gacatcaaca
    24901 gaggcaatct caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat aatgcgcaac
    24961 atggtatgct ccaggccgaa aaagcctagc gtaatggaat gatatcgcct tgccaaccct
    25021 gtgatgaacg ctgaccgact aacaataatg aaccacgatc ggcgcgaacg atgagacgac
    25081 catattcatc ccatagtgca ctgttaccac gggcatttgc catcagcacg gcaatcgagt
    25141 actgatgtga aaaaaattgt aagcgacggg ccgacgccag caggtcgggt tctcccagac
    25201 attgccctgt cgtaaacagt gaacaggtcg ggtccatatc cataccttgc ggttgttcat
    25261 cgaccaccgt gatggttctg gaacgacggc ccagacatgc accatgactc tgatggtaga
    25321 tcccaggcgt ttttcgccac ggtgcgaaca ccgcaattcc aggaataaag cgatcgttat
    25381 attcaacggg aaggccggca atgatggtca ttcgccaggt cgttgcggca taacaaagcg
    25441 ggtctaacag tgaaagatcg ggtggggcag gaagggcacg tcgcgaataa tcacacccca
    25501 gtaaagaaag cgatggaaaa actaacagct gacattgttg cctggcggcg gccctcacaa
    25561 actccagatg atgagctacc tgttcggtga gtgaggtttt acgaggctca tattgcgctg
    25621 ctgcaacttt ccagaacgac ataatgactt ccttttcaaa atgactgcaa attatggggg
    25681 cggtttagaa aatactcata atttagcagg cgttatgtaa gaaagtgtaa ctctgtataa
    25741 gttttattta agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag ccaaacggtg
    25801 ggcaataagc cgctcgcgaa accagctgcg caaatgttct ggctgttcac gttcaaccgc
    25861 ttcagcgatc accggcatat tgtagcgttc tttaaaagcc accccggccg ccgccaaatc
    25921 gacattcact ttatccattt ctgtttgatc cagtttagcc agattcttca gcattcttaa
    25981 tctcctcatc attcgcgggc aaaagttact aagagtgagc ctggatggca agccggaatt
    26041 gatgatcctc gactcagaca ttagacaagg ttatgctctg taaaaaagtt attacaaaaa
    26101 ggaatagata atatggcttc aactgcacgc tcccttcgct acgcgctggc aatcctgacc
    26161 acttcgttgg tgacaccttc cgtctgggca catgcgcatt taacgcatca gtatcctgcg
    26221 gcaaacgcgc aagtgacagc tgccccgcag gcaatcactt taaacttctc ggaaggcgtt
    26281 gaaacaggat tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat taaaacatta
    26341 ccagcgaagc ggaatgagca ggaccaaaag caactgattg ttccactggc cgattcgctg
    26401 aaacccggaa cctacaccgt tgactggcat gttgtttcgg tggacgggca taaaacgaaa
    26461 ggacactaca cctttagcgt gaaataaatc atgctggcgt ttacctggat cgcgctgcga
    26521 tttatccatt tcacctcgtt gatgctggtc tttggctttg caatgtatgg tgcctggctg
    26581 gcccccttaa tgattcgtcg cttgctgacg aagcgttctc tgcgtttaca acagcacgct
    26641 gccgtctgga gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga
    26701 acgggatgga cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt
    26761 ggtggcgtct ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg
    26821 caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg
    26881 gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacag cgaaaatcca tcagactaat
    26941 cacgcgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg
    27001 tgtatgcaac tcatcaaagg ccgttggcga caccaggcta ttcaggcgct gatgcgtttt
    27061 tcctggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct taatgcattg
    27121 ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa
    27181 gccatcttgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg
    27241 cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg tgtggatgac taaactcgaa
    27301 tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt
    27361 tgatggatta cgaaaatgat gaaaaaaagt attctggcgt ttctgttact caccagttct
    27421 gcagcggcgc tggcggctcc acaggtgata accgtgagtc gttttgaagt gggtaaagac
    27481 aaatgggcgt ttaatcgcga agaggtaatg ctgacttgcc gaccgggtaa tgctttgtat
    27541 gtcatcaacc caagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt
    27601 gccagtggga agaccaatgc ccaacccatt tcggtgattc agattgatga tcctaacaat
    27661 cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta
    27721 cctaaaatag ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg
    27781 cggtttttaa catttaagct gatgaccacc gcgcttttta ttgaccattt tgcacgcaaa
    27841 ctggaaaacc tggcgtcgtc atctattctt aaagggcaag gcaactaagc ctgcattaat
    27901 gccaactttt agcgcacggc tctctcccaa gagccatttc cctggaccga atacaggaat
    27961 cgtattcggt ctttttttaa ttgcatttga aatcaattag ttgcaaatgt ctcccacgaa
    28021 attccccgaa atttactcga atttctgtat tccggtcttt ttggttatat cacaaccaaa
    28081 atacatttaa caatccattt acgttaaaat cagagcagta agtacgtttt ttctctctca
    28141 tcaagataca tttttgttgt cttctccgat gtgtggccaa gtagacgctg agcaaattct
    28201 tctccacatg tttctttgta caatcgtcca gccaggcttc tgatctcgtg aaaagttggt
    28261 gggttttcac tgaactggat acctgtgaat tttctggctg cgacaaattt ttttgtcagg
    28321 ccatccgggt gaatgctgcc gtcagggctg ttttttctaa tcccggcact gattagataa
    28381 tctccccggc ttaccaggcg gcatcgttca actactgtac caagccgtag accagcgaca
    28441 ggaaggctga gtgacagggg gatagcaatc a
//
LOCUS       DADLSE010000041        27699 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLSE010000041 DADLSE010000000
VERSION     DADLSE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.041"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..613
                     /locus_tag="JN692_001762"
     CDS             <1..613
                     /locus_tag="JN692_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7264092.1"
                     /translation="MDIHSYHCEGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQ
                     ELTEVAELITSPEAIKNNLAVAFLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHAS
                     LQQRWSVADMAATIPCSEAWLRRLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGE
                     VADTLNFFDSFHFSKAFKHKFGYAPSAVLKNTDQHPTDASPHN"
     gene            complement(610..1424)
                     /locus_tag="JN692_001763"
                     /pseudo
     CDS             complement(610..1424)
                     /locus_tag="JN692_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(1424..3040)
                     /locus_tag="JN692_001764"
     CDS             complement(1424..3040)
                     /locus_tag="JN692_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAY7264093.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3335..4009
                     /locus_tag="JN692_001765"
     CDS             3335..4009
                     /locus_tag="JN692_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7264094.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(4048..5709)
                     /locus_tag="JN692_001766"
     CDS             complement(4048..5709)
                     /locus_tag="JN692_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7264095.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQQVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN692_001767"
     CDS             complement(5905..6333)
                     /gene="ibpB"
                     /locus_tag="JN692_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7264096.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN692_001768"
     CDS             complement(6445..6858)
                     /gene="ibpA"
                     /locus_tag="JN692_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7264097.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            7163..7495
                     /locus_tag="JN692_001769"
     CDS             7163..7495
                     /locus_tag="JN692_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7264098.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7497..8747)
                     /locus_tag="JN692_001770"
     CDS             complement(7497..8747)
                     /locus_tag="JN692_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7264099.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            8812..9912
                     /gene="cbrA"
                     /locus_tag="JN692_001771"
     CDS             8812..9912
                     /gene="cbrA"
                     /locus_tag="JN692_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HAY7264100.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSDAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(9873..11210)
                     /locus_tag="JN692_001772"
     CDS             complement(9873..11210)
                     /locus_tag="JN692_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264101.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN692_001773"
     CDS             complement(11285..12433)
                     /gene="dgoD"
                     /locus_tag="JN692_001773"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAY7264102.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPFGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(12430..13047)
                     /locus_tag="JN692_001774"
     CDS             complement(12430..13047)
                     /locus_tag="JN692_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7264103.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13031..13909)
                     /locus_tag="JN692_001775"
     CDS             complement(13031..13909)
                     /locus_tag="JN692_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7264104.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN692_001776"
     CDS             complement(13906..14595)
                     /gene="dgoR"
                     /locus_tag="JN692_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7264105.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14873..15529
                     /locus_tag="JN692_001777"
     CDS             14873..15529
                     /locus_tag="JN692_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264106.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN692_001778"
                     /pseudo
     CDS             complement(15575..16388)
                     /gene="yidA"
                     /locus_tag="JN692_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
     gene            complement(16502..16909)
                     /locus_tag="JN692_001779"
     CDS             complement(16502..16909)
                     /locus_tag="JN692_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7264107.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN692_001780"
     CDS             complement(17130..19544)
                     /gene="gyrB"
                     /locus_tag="JN692_001780"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7264108.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN692_001781"
     CDS             complement(19573..20646)
                     /gene="recF"
                     /locus_tag="JN692_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7264109.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPEFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN692_001782"
     CDS             complement(20646..21746)
                     /gene="dnaN"
                     /locus_tag="JN692_001782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7264110.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN692_001783"
     CDS             complement(21751..23154)
                     /gene="dnaA"
                     /locus_tag="JN692_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7264111.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23451..23531
                     /locus_tag="JN692_001784"
                     /pseudo
     CDS             23451..23531
                     /locus_tag="JN692_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23761..23901
                     /gene="rpmH"
                     /locus_tag="JN692_001785"
     CDS             23761..23901
                     /gene="rpmH"
                     /locus_tag="JN692_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7264112.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23918..24277
                     /gene="rnpA"
                     /locus_tag="JN692_001786"
     CDS             23918..24277
                     /gene="rnpA"
                     /locus_tag="JN692_001786"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7264113.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24241..24498
                     /gene="yidD"
                     /locus_tag="JN692_001787"
     CDS             24241..24498
                     /gene="yidD"
                     /locus_tag="JN692_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7264114.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24501..26147
                     /gene="yidC"
                     /locus_tag="JN692_001788"
     CDS             24501..26147
                     /gene="yidC"
                     /locus_tag="JN692_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7264115.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26253..27617
                     /gene="mnmE"
                     /locus_tag="JN692_001789"
     CDS             26253..27617
                     /gene="mnmE"
                     /locus_tag="JN692_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7264116.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEACQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6699 a         7465 c         7050 g         6485 t
ORIGIN      
        1 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
       61 tacccccacc agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga
      121 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
      181 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta
      241 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
      301 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
      361 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
      421 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
      481 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
      541 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
      601 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
      661 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
      721 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
      781 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
      841 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
      901 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgag
      961 gcttctgcga caattgctgc cgggttggag tagttgagca tccaggcatt tggtgaatat
     1021 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
     1081 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
     1141 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
     1201 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
     1261 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
     1321 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
     1381 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
     1441 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
     1501 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
     1561 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
     1621 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
     1681 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
     1741 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
     1801 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
     1861 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
     1921 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
     1981 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
     2041 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
     2101 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
     2161 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
     2221 ccgggaacaa cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
     2281 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
     2341 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
     2401 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
     2461 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
     2521 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
     2581 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
     2641 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
     2701 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
     2761 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
     2821 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
     2881 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
     2941 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
     3001 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
     3061 attctgtaag taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat
     3121 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
     3181 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
     3241 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
     3301 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
     3361 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
     3421 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
     3481 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
     3541 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
     3601 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
     3661 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
     3721 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
     3781 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
     3841 tggcggtaat tatgaaagcc tgacgccggt gctcgccgat agactgctgg cgcggcaaat
     3901 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
     3961 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
     4021 tttacggcga ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa
     4081 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
     4141 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
     4201 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
     4261 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
     4321 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
     4381 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
     4441 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
     4501 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
     4561 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
     4621 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
     4681 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
     4741 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
     4801 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
     4861 agacagcgag gtatcgacct cctgaccaat cacctgttgc gcattatgta aatccgctgg
     4921 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
     4981 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
     5041 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
     5101 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
     5161 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
     5221 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
     5281 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
     5341 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
     5401 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
     5461 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
     5521 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
     5581 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
     5641 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
     5701 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
     5761 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
     5821 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
     5881 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
     5941 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
     6001 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
     6061 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
     6121 ttttacgctc aggcgcgtac cttccagttg aatctctaaa tcttcctgac ggaaacctgc
     6181 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
     6241 gctctgactt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
     6301 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
     6361 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
     6421 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
     6481 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
     6541 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
     6601 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
     6661 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
     6721 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
     6781 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
     6841 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
     6901 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
     6961 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
     7021 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
     7081 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
     7141 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
     7201 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
     7261 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
     7321 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtgatggaca cgcttttact
     7381 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
     7441 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
     7501 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
     7561 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
     7621 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
     7681 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
     7741 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
     7801 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
     7861 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
     7921 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
     7981 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
     8041 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
     8101 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
     8161 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
     8221 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
     8281 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
     8341 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
     8401 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
     8461 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
     8521 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
     8581 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
     8641 tggctgtcgg gcgtccaggt attggtattg gtgagcaggt gattacgggg agcaaaggtt
     8701 atctgtttca tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac
     8761 ttcactttca gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat
     8821 ttcgacgtgg cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc
     8881 ggcaaaatgc aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc
     8941 aaaccttgcg gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg
     9001 acgcttcctg tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc
     9061 gccgcatcgc tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc
     9121 gacttgtgga tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc
     9181 cgaaaaatct ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag
     9241 caacatatta ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga
     9301 catctctacc cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag
     9361 aaacatccgg tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca
     9421 tggagtatca gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt
     9481 cagacgcgtt tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa
     9541 gcggtgaaaa gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt
     9601 ggtaaggaca acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg
     9661 gaggggatta gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag
     9721 ccggagaaga gcgacgccgc ttactggcgc gccacacgca aactgcgttt aaaactcttc
     9781 ggcaagatag taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc
     9841 agtggtgtgg cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc
     9901 agcaggatgt aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc
     9961 gcgaaaccgt aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga
    10021 ccaccggcga agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa
    10081 gagaccagcg accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg
    10141 cacataatca tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc
    10201 aagccgcaga taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc
    10261 agatccgcga cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg
    10321 gtcataaagc ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac
    10381 caggttaaga aaaaccacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc
    10441 agtttacgat ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc
    10501 ttcaccggcg catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct
    10561 ttgctgatac ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag
    10621 ataatgccga taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc
    10681 cagatcagca gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca
    10741 acggcggaag cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg
    10801 aaagcgggcg cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc
    10861 gtggcaaagc cctggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc
    10921 acgcgagaac ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc
    10981 caagcgaagg ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga
    11041 atatgggcgg aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata
    11101 aagatcatca ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata
    11161 tccatagcga gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat
    11221 ttacagaggg ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca
    11281 ggaattacca ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg
    11341 gggcattttt actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt
    11401 tcgttaacgg tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt
    11461 cgagtaactc cgcgcctttg ttgtaatgaa tgcccatact ttgttcctga agtaccgcgt
    11521 tataggaaac aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt
    11581 gcggcgcaag ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa
    11641 taccgcctgc atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc
    11701 gtttaaaatc gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg
    11761 ccgccaattt cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg
    11821 ggcgatacgg ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt
    11881 ggaaatcaag accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt
    11941 tgactgccgc atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca
    12001 gtttgaaggt atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg
    12061 gacgatcgcc gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca
    12121 gttgccagac tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc
    12181 cggcgatggc gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt
    12241 gccataagtc gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa
    12301 ctgccgcttc caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc
    12361 cttcatcggt ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt
    12421 tggtgatttt cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc
    12481 ggtgcgttct acggactgcc cggcgcgata gagatcgctg cctaagcccg ccccaacaca
    12541 acctgcgtct atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc
    12601 gatgtccggt ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga
    12661 cggaaatatt ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt
    12721 cgcgcagccg gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg
    12781 gatattgggc gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg
    12841 tttcagtacc gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg
    12901 aatgctctgc tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc
    12961 aatcaccgcg ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat
    13021 cagcgggagt ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg
    13081 tgtcgcccgc caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg
    13141 cggtcagcga tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca
    13201 tactggcaac ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg
    13261 gaagtgttcc cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg
    13321 cattaaggcc gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca
    13381 aacctgcgcc aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg
    13441 tgcgaaaatc gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca
    13501 taacataaag agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc
    13561 gcatcacatt gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt
    13621 cgccaacaga cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg
    13681 gagcaacttt ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt
    13741 cttcacgcca gtcggtcgtg acttctgcta acaccgcagc cggagatttt ccgttcaggc
    13801 gcgtgacgcc tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg
    13861 cgcgcagatt ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt
    13921 taaccttcgt gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc
    13981 ctgatgccgt atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc
    14041 ctcatcgccc atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct
    14101 aagttgctgt aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc
    14161 cgcttcgtta aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc
    14221 aatctgcgcc agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc
    14281 caccagattt cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag
    14341 cacccattgc agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc
    14401 cccgcgataa cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg
    14461 gatgatgttg cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga
    14521 gccaggtacg tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt
    14581 tttattgaga gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat
    14641 cgatgcataa cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgtgtgt
    14701 gtaatgtggt acaaacttat gttgttgtac tacaatttag atcaaaaaaa gaacaatgcg
    14761 taaaaaaatg acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag
    14821 ggaaagaaga gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt
    14881 gaattttaag ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg
    14941 ctgtatctcg tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc
    15001 gcagtcagac accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta
    15061 tgtttttgta ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa
    15121 gatgctcaat gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt
    15181 tgttttaaat gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa
    15241 taacgaagaa gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg
    15301 taccaggtca ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta
    15361 ctcaaaggtg atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc
    15421 cagaggcatt ccggatggtg tttccgcagc attactgcca gtgactgtta cgctggacat
    15481 cattactgca ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg
    15541 gcaaacgcgt ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag
    15601 caaacgccac gccatcttca atggttggat tttgtgacaa agttcgccac ttctttcacc
    15661 gaaggaatag cgttatccat cgccacaccg acgcctgcat attcaatcat tgcgatgtcg
    15721 ttttcctgat cgccaatcgc catgatttct tccggtttaa tacctaacac gtcggccagt
    15781 gatttcacac ccgtaccttt gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca
    15841 cttttcagca cggtatattt ctctttcact tcctgcggaa tacgcgcgat agcctggtcg
    15901 aggatggcgg gttcatcaat catcatcact ttcaggaact gggtattggg gtccattttc
    15961 tccgcttcgc agaacaccag cggaattgtg gcaacgaagg attcatgcac cgtgtagtag
    16021 ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca gggcgtggaa atgagaaccg
    16081 acttcgcgag agagtttttc caggaaacga tagtcgtcat agctgagagc ggtttgcgcc
    16141 acggtgctac catcagcggc cttctgtacc agcgcgccgt tataagtaat gcagtagtcg
    16201 cccggctgtt ccatatgcag ctctttcagg tagttgtgca cacctgcata cggacgaccc
    16261 gtcgttagca cgacattcac gccacgggcg cgagctgcgg caatcgcatt tttaacggcg
    16321 ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca tatcgatagc aatgagtttg
    16381 atagccatga gttcccaagg tagattagtt cctgacccat gctaacgcga ttccgctcaa
    16441 aaatcagtac aacacccgag ggaaaagggg atgcaacgcg ggttgcatgc tccccatttg
    16501 cttagcggaa gagtttccct ttcagcagtt ccatgcctgc ggaaagcaga tcgttattgg
    16561 cttgtgctga cacttcacct tgcggtgaga gcgcatcaat aatcttcggc aattgctctg
    16621 ccagtaaact ggaagccgta ctggtatcca cgccaagttt ttgcccgaga tcggacaccg
    16681 catttgtgcc gagcgccgat tccagttgct cgccgctaac tgattgattt ctctgttgat
    16741 tactcaacca ggttgagaga atggccccta agccgccact ttgcagtttt tccagcagca
    16801 cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc ctgatatttc cccgcatcgc
    16861 ctttcagaaa ggcaccgaca acttcatcaa aaagccccat gataatcacc tgtaaggcgt
    16921 tacgtgttga cccaaaaagt atagatctgc gggatgataa ttgcggattg cagaaataaa
    16981 aagggcggag atgatctccg cccttttctt atagcttctt gccggatgcg gcgtgaacgc
    17041 cttatccggc ctacaaaatc aatatattgc aggagtctcg taggactaat aagcgtagcg
    17101 catcaggcac gctcgcatgg ttaacgccat taaatatcaa tattcgccgc tttcagggcg
    17161 ttctcttcaa taaacgcacg acgcggttct acggcgtcgc ccatcagcgt ggtgaacaac
    17221 tggtcagcgg caatcgcatc cttaacggta acgcgcagca tacgacggct ttccgggtcc
    17281 atggtggttt cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg
    17341 gagaggccac gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc
    17401 ggctgacgac gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc
    17461 tcacccagcg tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga
    17521 tagtcagtat ccacaccgtg ggtacgcacg cgaacaatcg gctcgaacag gttttgttcg
    17581 gcattggtgt ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg
    17641 ctgaccagcg cgttcaccca gcgggtaacg gtctgctcat cagaaaggtc agcttccgtc
    17701 aacgtcggct gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg
    17761 ttgatcattt tctgcgtcgc gttgtactca gataccagtt tctctaacgc ttcgccagcc
    17821 aatgccggtg cactggcgtt ggtgtgcagc gttgcgccgt ccagcgcgat agagatctgg
    17881 tactgatcca tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg
    17941 tacagcggcg gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga
    18001 tagaagaagg tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg
    18061 atgatgatgc tgtgataacg tagtttgtcc gggttgtact cgtcacgacc gataccacag
    18121 ccaagcgcgg tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct
    18181 ttctcgacgt taaggatttt acccttcagc ggcagaatcg cttggttctt gcggttacgc
    18241 ccctgcttcg cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc
    18301 ggatcgcgtt cctggcagtc tgccagtttg cccggcaggc cagctaagtc gagcgcacct
    18361 ttacggcggg tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata
    18421 attttgccaa ccacgatttt cgcatcagtt gggttttcca gcaggtattc cgccagcagt
    18481 tcgttcatct gctgttcaac cgccgatttc acctcagaag aaaccagttt gtctttggtc
    18541 tgggaggaga atttcgggtc cggcactttt acggaaacga ccgcaatcag gccttcacgc
    18601 gcatcgtcac cggtggcgct gactttggct tttttgctgt agccttcttt gtccatgtag
    18661 gcattcaggg tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc
    18721 tgcggaatgt tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcagc
    18781 gccacttcaa cgccaatacc gtctttttcg gtggagaagt agaagatatt tgggtggatc
    18841 ggcgttttgt tcttgttcag atattcaacg aacgctttga tgccgccttc atagtggaag
    18901 tggtcttctt tgccgtcacg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac
    18961 gacaactcac gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag
    19021 gtttcgaggc tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc
    19081 gccagcggag cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc
    19141 tgaataacca gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc
    19201 agaccgccgg acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc
    19261 atgatcactt ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc
    19321 ccgtcatcct gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag
    19381 tgacccgcga gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga
    19441 ccggtgccgt catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc
    19501 cctttcagga ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca
    19561 tttatacttg ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc
    19621 gtccgacatg tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt
    19681 cgcttttaag cggctggcaa gcaacccgcg acgctcatca tcaagctcag aggcaaaatc
    19741 atctatcagg tagagacacc gccgcccgct ttcacgagtg aggaactctc cttgcgccag
    19801 acgtaaggcg cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc
    19861 accgtcggcg cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg
    19921 gcgatcgcgt tcaaaattac gttccagcac ctcagcgtac tctgtctctt tctcccagcc
    19981 gcgctggaaa gagaaagtca gagaaaactc agggagaaat tgcttacagg tatcagccat
    20041 atcggccgcg ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg
    20101 gatcagttct ttatcccacg ggcgtagctg ttcgtaacga gtcacctggc gcagcgccgc
    20161 attgcgctgc ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatt caggttcgtt
    20221 gtgaaagcat ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt
    20281 aaacccttct ggcgttatca actgcatcgg catcaggtgc gccagttccg cgactttatg
    20341 cccgtccgta ccgtcaatac ggactttgct gtcgccctgt ttgtctttgg ttaagccaat
    20401 cgccgtctcg cgctcttcgc cctgtaatcg cccgtggaga acaaatgcct cctgctcatg
    20461 gcgaatgacg cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat
    20521 ggcttccagc acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg
    20581 agataaagcg agatccgcgg tttcaatatt gcggaaatcg cggatcaaca agcgggtgag
    20641 ggacattaca gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca
    20701 atctgcacgc tggaaaccga atcggtcagc atcatgcgga cgttttcgca tttcagcgcg
    20761 ttcagaacat ccagcacata gctgacgttg aagccgattt ccatctccgc accgctatag
    20821 gtaacgtcga ggatctcttc cgcttcttcc tgttccgggt tgttggcagt aattttcagc
    20881 tggttttcgc tgacatacag gcgcacaccg cggaatttct cgttagagag aatcgccgcg
    20941 cgagcaaacg cctgcttgag cagatcgcag cctgcttcca gatgtttgtc cgggttcttc
    21001 ggcagaacgc gacggtaatc cgggaagcga ccatccacca gtttcgaggt gaaaataaag
    21061 tcgccaacgt gggcacgaat gttattgctg ccaatctgca cacgcagcag attgtcgccg
    21121 ccgtcgagca tacgcatcag ttcaatcacg cctttacgcg gtacgatcac cgaatggctt
    21181 ggcaaagatt gaccaattgg cattgaacag accgccagac ggtggccgtc ggttgccacg
    21241 gtgcgcagtt cttcaccttc ggtttcaaac agcataccgt ttaagtaata gcgaacatcc
    21301 tgatgcgcca tcgaaaactg ggttgcttca atcagacgct tcatcgtcgc ctgcggcagg
    21361 gtaaattcga cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta
    21421 gacagcgaaa aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgcacg
    21481 gcaatttccg cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg
    21541 gtcgtcgctc ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg
    21601 agatcagtac cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga
    21661 atcggtagcg taggacgacc acctaacgga ccgctcacct gttgtagcgg ttttaataaa
    21721 tgctcacgtt ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga
    21781 aaaatcttct ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg
    21841 aagcaccgtc gtgtggtcac gcccaccaaa cgcatcgcca atctctggca gactgtggtt
    21901 agtcagctct ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg
    21961 acgcttggaa aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat
    22021 attgtcgatg gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagcg cctcacgcac
    22081 gaagtcgatg gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc
    22141 cccttccagc tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcacc
    22201 cggcaaacga atgtcatttt cgtcggcctt tttcatcagg atcgccacac gggtttccag
    22261 ctctggcggt tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc
    22321 aacgccgttg atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc
    22381 cagcagggcg ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa
    22441 ctgaatatcg tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat
    22501 cgcgttgttt tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac
    22561 cactttggca ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt
    22621 tttacccaga cccgtgccgc cataaaggaa caacgggtta taggcaccgc cagggttatc
    22681 cgccacctgg cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc
    22741 aaacgtgtgt ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt
    22801 atcccaacct gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc
    22861 aggggccgcg acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc
    22921 gacttcaaaa cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat
    22981 attattaagg tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc
    23041 cagcgtgtta tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc
    23101 tggtaactca tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga
    23161 ctccactcga acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac
    23221 gtcgaaaaga ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat
    23281 caagatcctg caaaacgctc gggaccgcgg atcatagcct aaactgcgca agagatcttc
    23341 tgtttctcac agattttctc tatttatcca caggactttc cagaactcgc taagtgtaaa
    23401 cgatcctgcc gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag
    23461 acaaaaattg gcttaattga tctaataaag atccaggacg atccttgcgc tttacccatc
    23521 agcccgtata atcctccacc cggcgcgcca tgctggtttc cactggtgtg aggtcgtaca
    23581 ttttccctgc gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccggt
    23641 gtgtacaatt attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc
    23701 atttttcggc ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc
    23761 atgaaacgca cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct
    23821 cgtatggcta ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct
    23881 cgtctgaccg tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag
    23941 ggagttacgc ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc
    24001 tggcacgccg caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg
    24061 tcttacagtc gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct
    24121 gacgcgtgaa agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt
    24181 ggcgaaaaaa ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt
    24241 atggcgccgc cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa
    24301 cgcctgatta gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac
    24361 ggaattgagg cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc
    24421 gtattaaaat gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt
    24481 gataccagag aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt
    24541 tcgtgtcttt catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc
    24601 aacagaccac gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg
    24661 ccagtggcca ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca
    24721 cccgtggtgg tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta
    24781 cccagccgtt ccagctgttg gaaacttcac cgcagtttat ttatcaggca cagagcggtc
    24841 tgaccggtcg tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg
    24901 aaaaagacgc ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata
    24961 ccgacgcggc aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg
    25021 tcaacgtcaa ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tcctcgtttg
    25081 gtcagttgaa gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg
    25141 cactgcacac cttccgtggc gcggcgtact ccacgcctga cgagaagtat gagaaataca
    25201 agttcgatac cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg
    25261 cgatgctgca acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact
    25321 tctataccgc taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac
    25381 tggttcagcc tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc
    25441 aggacaaaat ggcagctgtt gctccgcacc tagatctgac cgttgattac ggttggttgt
    25501 ggttcatctc tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact
    25561 ggggcttctc cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca
    25621 aagcgcagta cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc
    25681 gtgagcgtct gggcgatgac aaacagcgta tcagtcagga aatgatggcg ctgtacaaag
    25741 ctgagaaggt taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc
    25801 tggcgttgta ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt
    25861 ggatccacga cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa
    25921 cgatgttctt cattcagaag atgtcgccga ccactgtgac cgacccgatg cagcagaaga
    25981 tcatgacctt tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg
    26041 tactgtacta tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg
    26101 gtctggaaaa acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt
    26161 cgctaaaata agggcggtca gttgaccgcc ttttttcttt tcgtagggcg gataagcacc
    26221 gcgcatccgc cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc
    26281 aggccacgcc tccgggacgt ggcggcgttg gcatcctgcg catctccggc ttcaaagccc
    26341 gtgaagttgc cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc
    26401 cgtttaaaga cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc
    26461 cgaactcgtt caccggcgaa gatgtgctgg aactgcaagg tcatggcggt ccggtgatcc
    26521 tcgacctgct gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg
    26581 agttttccga acgcgcgttt cttaacgata aacttgactt agcccaggcc gaggcgattg
    26641 ccgatcttat cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag
    26701 gcgcattctc cgcacgggtt aatcatctgg tagaagccct cacccacttg cgcatttacg
    26761 tcgaagcggc aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg
    26821 aagcccagct taatgacgtg atggccgatc tcgatgcagt gcgtgctgaa gcatgtcagg
    26881 gtagtttgtt gcgcgaaggg atgaaagtag tgattgccgg acgtcctaat gccggtaaat
    26941 cgagcctgtt aaacgcgctg gcgggccgtg aagcggcaat cgtaaccgat atcgccggaa
    27001 ccacgcgtga cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg
    27061 acactgccgg gctacgtgag gccagtgacg aagtggaacg tattggtatc gagcgcgcgt
    27121 ggcaggaaat tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acgacagacg
    27181 ccgttgatcc ggcagagatc tggccggaat ttatcgcccg tctgcccgcg aaactgccga
    27241 tcaccgtggt acgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga
    27301 acggtcacgc gttaattcgt ctctcggcaa ggactggtga aggcgtggac gtgctgcgta
    27361 accatctcaa acagagcatg ggctttgaca ccaatatgga aggcggcttc ctggcgcgcc
    27421 gtcgccactt acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac
    27481 tgttgggagc ctgggcaggt gaactgctgg cggaagagtt gcgcctggca cagcagaact
    27541 taagcgaaat caccggggaa tttacttcag acgacctgct ggggcggatt ttctccagct
    27601 tctgtattgg taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg
    27661 cttcattgtt agcactcctg ttatgcctca acccttttt
//
LOCUS       DADLSE010000042        26913 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLSE010000042 DADLSE010000000
VERSION     DADLSE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.042"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            126..449
                     /gene="iraM"
                     /locus_tag="JN692_001790"
     CDS             126..449
                     /gene="iraM"
                     /locus_tag="JN692_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7264117.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIQIC
                     PYGLK"
     gene            complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN692_001791"
     CDS             complement(552..1802)
                     /gene="icd"
                     /locus_tag="JN692_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7264118.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1974..2627
                     /gene="rluE"
                     /locus_tag="JN692_001792"
     CDS             1974..2627
                     /gene="rluE"
                     /locus_tag="JN692_001792"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7264119.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTKRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGTELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2637..3098
                     /gene="nudJ"
                     /locus_tag="JN692_001793"
     CDS             2637..3098
                     /gene="nudJ"
                     /locus_tag="JN692_001793"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7264120.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3152..4258
                     /gene="mnmA"
                     /locus_tag="JN692_001794"
     CDS             3152..4258
                     /gene="mnmA"
                     /locus_tag="JN692_001794"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7264121.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4294..4935
                     /gene="hflD"
                     /locus_tag="JN692_001795"
     CDS             4294..4935
                     /gene="hflD"
                     /locus_tag="JN692_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7264122.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4939..6309
                     /gene="purB"
                     /locus_tag="JN692_001796"
     CDS             4939..6309
                     /gene="purB"
                     /locus_tag="JN692_001796"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7264123.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKNIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6478..7149
                     /gene="phoP"
                     /locus_tag="JN692_001797"
     CDS             6478..7149
                     /gene="phoP"
                     /locus_tag="JN692_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7264124.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7149..8609
                     /gene="phoQ"
                     /locus_tag="JN692_001798"
     CDS             7149..8609
                     /gene="phoQ"
                     /locus_tag="JN692_001798"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7264125.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAEESMLGGARMEVIFGRQHSAPKDE"
     gene            8685..9806
                     /locus_tag="JN692_001799"
     CDS             8685..9806
                     /locus_tag="JN692_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7264126.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN692_001800"
     CDS             complement(9855..11081)
                     /gene="pepT"
                     /locus_tag="JN692_001800"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7264127.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEALSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11331..12467
                     /gene="potA"
                     /locus_tag="JN692_001801"
     CDS             11331..12467
                     /gene="potA"
                     /locus_tag="JN692_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7264128.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12451..13308
                     /gene="potB"
                     /locus_tag="JN692_001802"
     CDS             12451..13308
                     /gene="potB"
                     /locus_tag="JN692_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7264129.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13305..14099
                     /gene="potC"
                     /locus_tag="JN692_001803"
     CDS             13305..14099
                     /gene="potC"
                     /locus_tag="JN692_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7264130.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14096..15142
                     /gene="potD"
                     /locus_tag="JN692_001804"
     CDS             14096..15142
                     /gene="potD"
                     /locus_tag="JN692_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7264131.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN692_001805"
     CDS             complement(15298..16119)
                     /gene="cobB"
                     /locus_tag="JN692_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7264132.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN692_001806"
     CDS             complement(16135..17046)
                     /gene="nagK"
                     /locus_tag="JN692_001806"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7264133.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN692_001807"
     CDS             complement(17075..18319)
                     /gene="lolE"
                     /locus_tag="JN692_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7264134.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN692_001808"
     CDS             complement(18319..19020)
                     /gene="lolD"
                     /locus_tag="JN692_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7264135.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN692_001809"
     CDS             complement(19013..20212)
                     /gene="lolC"
                     /locus_tag="JN692_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7264136.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPVQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20474..21547
                     /locus_tag="JN692_001810"
     CDS             20474..21547
                     /locus_tag="JN692_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7264137.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21675..25121
                     /gene="mfd"
                     /locus_tag="JN692_001811"
     CDS             21675..25121
                     /gene="mfd"
                     /locus_tag="JN692_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7264138.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25264..26226
                     /gene="ldtC"
                     /locus_tag="JN692_001812"
     CDS             25264..26226
                     /gene="ldtC"
                     /locus_tag="JN692_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HAY7264139.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPIGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN692_001813"
     CDS             complement(26309..26566)
                     /gene="bhsA"
                     /locus_tag="JN692_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7264140.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26808..>26913
                     /locus_tag="JN692_001814"
     CDS             26808..>26913
                     /locus_tag="JN692_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7264141.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQH"
BASE COUNT         6621 a         6946 c         6793 g         6553 t
ORIGIN      
        1 tttgttttat ataagcaatc gttttaatta atttattttt tgaaggggta atatactcat
       61 atgcaaaata aagaaatgaa catccaaatg aactatatta aataccgtgg gataagacat
      121 aacaaatgaa gtgggtagta attgacacgg taattcaacc tacatgcggc atatcttttt
      181 cagccatatg gggtgatatg aaaatgatca tctggtatca atctactata tttcttcctc
      241 ctggaaggat atttacaccg gttaagcttg gtattatcct taaggaaaaa gaatatccta
      301 ttactattta taacattgca ccattcaaca agaatttatg gagtttactc aaaagcagtc
      361 aagagtgtcc tccaggagaa agcgaaataa caaataaatg tttgcataag agttgtatta
      421 tacaaatatg cccatatggg ctcaagtaat aggttttata taaatatgct cgatatgcta
      481 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgttta aatatttaac
      541 acatgtagtg attacatatt cttgatgatc gcatcaccaa actctgaaca tttcagcagt
      601 ttagcgcctt ccatcagacg ctcgaagtca taggttacgg tcttggcgtt gatcgcgcct
      661 tccatacctt taacaatcag gtctgcggct tcagtccagc ccatatggcg taacatcatc
      721 tctgcggaga gaataataga gccagggttt actttgtcct gaccggcgta tttcggcgca
      781 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggggcgata
      841 ccgataccgc caacctgcgc tgccagagcg tcagaaatgt agtcaccgtt caggttcata
      901 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      961 atcacgtctt taatgacgat ctctttgccg gtattcgggt ttttaacttt cagccacggg
     1021 ccgccgtcga tcagttcacc gccaaattct tcacgcgcca gctggtagcc ccagtcttta
     1081 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagggtcac agagtcacga
     1141 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttcagaa
     1201 cacggcttaa taccgatacc gcaatgttcc gggaagcgaa ttttcttcac gcccatctct
     1261 tcacgcagga atttaatcac tttctcggcg tcggcagagt cagctttcca ttcgataccc
     1321 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1381 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1441 tcctgacgca gggcaacgtt gagagaacga ataccgccac caaccggagt ggtcagcggg
     1501 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1561 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1621 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1681 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1741 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1801 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1861 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1921 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1981 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac caggttaagc
     2041 gattcagctc gcaacgttct accaagcgta aacatgaaaa ccagcccacg cgtgtgatcc
     2101 tcttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     2161 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgccttgac cgcgatagcg
     2221 aagggttgct ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggta
     2281 aacgcaccgg aaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2341 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgca ggcacggagc
     2401 tggttgacga acccgcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2461 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2521 tgaccgccca tgttggtttc cccacgctgc gactgattcg ctatgcgatg ggagattact
     2581 ctttggataa tcttgccaat ggcgaatggc gagaagtgac agattaagga tgcaaaatgt
     2641 ttaaaccgca cgttaccgtt gcttgcgtgg tgcacgcaga aggcaaattt ttagtcgttg
     2701 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2761 aaaccttagt ggaagccgcc gcccgtgaac tgtgggaaga aaccggcatc agcgcgcagc
     2821 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2881 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2941 actgctgccg ttgggtcagc gccgaagaaa ttttacaggc gtcaaatctt cgttcgccgc
     3001 tggtggcgga aagtattcgt tgttatcaaa gcgggcaacg ttatccgctg gagatgattg
     3061 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     3121 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     3181 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     3241 tcaggtcgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3301 agcggcagcg gatctggctg atgcccaggc ggtctgcgac aagctcggca ttgaactgca
     3361 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3421 taaagccggt cgcacgccga atccggatat tctgtgcaac aaggagatca aatttaaagc
     3481 cttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3541 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3601 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3661 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3721 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttctt
     3781 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3841 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3901 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3961 tctggttgtc gctcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     4021 gcagctgcac tgggtcgatc gcgaaccatt caccggcact atgcgttgca cggtaaaaac
     4081 ccgctatcgc cagaccgaca ttccgtgcac cgtcaaggcg ctggacgacg atcgcattaa
     4141 agtgattttc gatgagccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     4201 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     4261 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4321 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4381 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4441 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4501 ctcggcgtgc tcaatgccag cagttgccag ggcttaaacg ccgaattaac ccgctacaca
     4561 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4621 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4681 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4741 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgcaggcgaa agttcgcgca
     4801 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtcta
     4861 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4921 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4981 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     5041 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     5101 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     5161 agcgaagaag atgccgcacg catcaaaaac atcgagcgta ccactaacca cgacgttaaa
     5221 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     5281 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5341 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5401 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5461 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggaacgccag
     5521 caccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5581 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5641 acctcgctgg gtattcaatg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5701 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5761 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5821 tcttccacca tgccgcataa agttaacccg atcgacttcg aaaactccga agggaatctg
     5881 ggcctttcca acgcggtatt gcagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5941 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     6001 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     6061 ctggatgagc tggatcacaa ctgggaagtg ctggcagaac caatccagac agttatgcgt
     6121 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc ttactcgcgg taagcgcgtt
     6181 gacgccgaag gcatgaagca gtttatcgat ggtctggcgt tgccagaaga agagaaagcc
     6241 cgcctgaaag cgatgacgcc ggctaactat attggtcgag ctatcacgat ggttgatgag
     6301 ctgaaataag cctcaaatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt
     6361 atccactttt tcttttttca ctttacctcc cctccccgct ggtttattta atgtttaccc
     6421 ccataaacac ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg
     6481 cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag
     6541 gatgctggtc atcaggtcga tgacgcagaa gatgccaaag aagccgatta ttatctcaat
     6601 gaacatttac cggatattgc gattgtcgat ctcggattgc ctgacgagga cggtctgtca
     6661 ctgattcgcc gctggcgtag caacgatgtt tcactgccga ttctggtatt aaccgcccgt
     6721 gaaagctggc aggacaaagt cgaagtatta agtgccggtg ctgatgatta cgtgactaaa
     6781 ccgtttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt
     6841 ctggcttcac aggtcatttc gctccccccg tttcaggttg atctctctcg ccgtgaatta
     6901 tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg
     6961 atacgcaata atggcaaagt ggtcagcaaa gattcgttaa tgctacaact ctatccggat
     7021 gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt
     7081 caggcacaat atccgcaaga agtcattacc accgttcgcg gccagggcta cctgttcgaa
     7141 ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc
     7201 tgttggcaac ggcagcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga
     7261 tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc
     7321 tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata
     7381 tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg
     7441 cgcaacgtga cgtgccctgg ctgatgaaaa tgatccagcc tgactggctg aaatcgaatg
     7501 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt
     7561 cgatacagca acagttgcag gaagtgcggg aagatgatga cgacgcggag atgacccact
     7621 cggtggcggt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg
     7681 tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg
     7741 tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt
     7801 tacgccccat cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat
     7861 tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt
     7921 taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata
     7981 gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga
     8041 tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa
     8101 ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc
     8161 atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca
     8221 aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg
     8281 attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg
     8341 tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc
     8401 ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt
     8461 tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg
     8521 gtaaaatcgt cgccgaagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc
     8581 gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc
     8641 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
     8701 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
     8761 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
     8821 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
     8881 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
     8941 taaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
     9001 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
     9061 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
     9121 aaaagctgca aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg
     9181 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
     9241 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggtttt cgcgcgccaa
     9301 atacgcggga attaattagc ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
     9361 actactacag cgatccggat gttccacctc gcgctcatcc tgcggatgtt ctgccgcaag
     9421 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
     9481 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
     9541 cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc
     9601 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
     9661 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
     9721 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
     9781 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcagc atcacatgcc
     9841 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
     9901 accgcttttt ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg
     9961 ccagtgaaca ggttcgggca aggtaatccc ataaacgaca actgcgcgcc gtcggtaccg
    10021 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcatcgcctg ctgggcgata
    10081 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
    10141 accaattcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
    10201 attttacgtt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
    10261 tcggcccgtt caacggtgcc tttcatgctc gccaggtgat agaaaccttc atagccttct
    10321 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    10381 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    10441 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    10501 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    10561 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    10621 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    10681 ttaccatcgg tggtaatcaa cgtctgaccc agtagctgat gcagcaccgg gaacataacc
    10741 ggtgataaag cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca
    10801 atttgcggat taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag
    10861 ccaatcgccg ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc
    10921 tcacttaagg tcacattgat aagccccatt tcttcgagct gctctttcag cagatgcaat
    10981 aacttccatt ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc
    11041 agagacacgt agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt
    11101 gtgacaacat tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct
    11161 gcaagcggga atatttatca gcattacctt aatgaatata aagctaagcc agtaattcac
    11221 aacaaagatt ggcgattcaa gcggtttgcc gtagaattac cgccctcatt aagagtcacc
    11281 aaggtggtta accacaaacc ccgcatcggt aagccatccg ttgcgtttac atgggacaga
    11341 gtaaaaaatt gaataaacaa ccgagttcgc tttcaccgct ggtgcaattg gcgggaattc
    11401 gcaaatgctt tgatggtaaa gaggtcattc cccagctgga tctgactatc aacaatggcg
    11461 agttcctcac gctgcttggc ccttctggct gcggtaaaac aaccgttctt cgcctgattg
    11521 caggtctgga aactgttgat tccggacgca tcatgctgga taacgaggac atcacccacg
    11581 ttccggcgga aaaccgctat gtgaacactg ttttccaaag ctatgcactt ttcccccaca
    11641 tgaccgtgtt cgaaaatgtg gcctttgggt tgcgcatgca aaaaaccccc gctgctgaaa
    11701 ttacgccccg cgtgatggaa gccctgcgga tggtgcagct ggaaaccttc gctcaacgta
    11761 aaccgcatca gctctctggt ggtcaacagc aacgcgtcgc cattgctcgc gcggtggtta
    11821 acaagcctcg tctgttgttg ctggatgagt cgctctcagc gctggattac aaactgcgta
    11881 agcaaatgca gaacgagctg aaagcgttac agcgtaagct tggcattaca ttcgttttcg
    11941 tgactcacga ccaggaagaa gcactcacca tgtcggacag gattgtggtg atgcgcgatg
    12001 gtcgcattga gcaagacggc acgccgcgtg aaatctacga agagccgaaa aacctgtttg
    12061 ttgccgggtt cattggcgaa attaatatgt ttaacgccac ggtgatcgag cgactcgacg
    12121 agcagcgcgt acgcgccaac gttgaaggcc gcgaatgtaa tatctacgtt aacttcgccg
    12181 ttgaaccggg gcaaaaactg catgttctgc tgcgcccgga agacttacgt gttgaagaga
    12241 ttaacgacga caaccacgct gaagggctga ttggttacgt tcgtgagcgt aactacaaag
    12301 gcatgacgct ggagtcggtt gttgaactgg aaaatggcaa gatggtgatg gtcagcgaat
    12361 tcttcaatga agacgatcct gactttgacc actctctcga ccaaaaaatg gccattaatt
    12421 gggtagaaag ctgggaggtc gtactggctg atgaagaaca caagtaagtt ccagaatgta
    12481 gtgattgtca ctattgtcgg ttggcttgtg ttgtttgtct ttctgcccaa cctgatgatc
    12541 attggcacca gctttttgac ccgcgacgac gccagtttcg tcaaaatggt ctttacgctg
    12601 gataactaca cgcgtctgct cgatccgctc tattttgaag tgctattgca ctcgctgaat
    12661 atggcgctga tcgccaccct cgcctgcctg gtgctgggct atccatttgc ctggtttctg
    12721 gcgaagctgc cacacaaggt gcgtccgctg ctgctgtttc tgctgattgt tccgttctgg
    12781 accaactcat tgattcgtat ctacgggctg aaaattttcc tcagcaccaa aggctatctc
    12841 aacgagtttt tgctctggct gggcgttatc gacacaccaa tccgtatcat gttcacgccc
    12901 agtgcggtga ttatcggtct ggtttacatt ctgctgccgt ttatggtgat gccgctgtac
    12961 tccagtatcg aaaaactgga taagccgttg cttgaggcgg cgcgcgatct cggtgccagc
    13021 aagttacaga cttttatccg catcattatt ccactgacga tgccggggat tattgccgga
    13081 tgtctgctgg tgatgctgcc agcgatgggc ctgttctatg tgtccgacct gatgggcggt
    13141 gcgaaaaacc tgctgatcgg taacgtcatc aaggtacagt tcctcaatat tcgtgactgg
    13201 ccgtttggtg cagccaccag cattacgctg actatcgtaa tgggcctgat gttgctggtt
    13261 tactggcgcg cttctcgttt gctgaataag aaggtggaac tcgaatgatc ggtcgactgc
    13321 ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc attattttga
    13381 ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagggtttt accaccaaat
    13441 ggtatagcct gctgatgaac aacgacagcc tgttacaggc agcgcagcat tcactaacaa
    13501 tggcggtgtt ttcggcgacg tttgctacgc ttatcggttc actgacggca gttgcgctgt
    13561 accgttatcg ctttcgcggt aagccgttcg ttagcggaat gctgtttgtg gtaatgatgt
    13621 caccagatat cgtgatggca atttcgctgc tggtgctgtt tatgctgctg ggtattcagc
    13681 ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccattt gtggtggtga
    13741 cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct ggaagcggcg aaagatctcg
    13801 gtgccagcga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg ccagcggtgg
    13861 cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtagtt tcttcgttcg
    13921 tcaccggacc aagttatgaa attctgccgt taaaaattta ttcgatggtc aaagtcggcg
    13981 tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg gtgatggtta
    14041 ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac gttaaatgaa
    14101 aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga gcgccgctca
    14161 cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc cgccaggact
    14221 gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt acgagtcgaa
    14281 cgaaaccatg tacgccaagc tgaaaacgta caaagacggt gcctatgacc tagtggttcc
    14341 ttcaacctat tacgtcgata aaatgcgtaa agaggggatg atccagaaga tcgacaagtc
    14401 gaagttaacg aacttcagca atctcgatcc agacatgctc aacaagcctt ttgacccgaa
    14461 taacgactac tccattccgt atatctgggg tgcgacggcg attggtgtta acggtgatgc
    14521 ggtggatccg aaatctgtca ccagctgggc cgatctgtgg aagccagagt acaaaggcag
    14581 cctgctgttg accgacgatg cccgtgaagt gttccagatg gcgctgcgta agctgggcta
    14641 ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc tgaaaaaact
    14701 gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca tggaaggcga
    14761 agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg cgggtacgcc
    14821 tattgacgtg gtgtggccga aagaaggcgg catattctgg atggacagcc tggcgatccc
    14881 ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc tgcgcccgga
    14941 tgtggcaaaa caggttgctg aaactatcgg ttatccaacg ccaaaccttg cggcgcgtaa
    15001 gctgttaagt ccagaagtgg caaacgataa gacgctctac ccggatgctg aaaccattaa
    15061 aaatggcgaa tggcagaatg acgttggcgc agccagcagc atttatgaag agtattatca
    15121 gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg gtattaccgc ccgtttttta
    15181 gttagcaagg atgtggctaa tgagtcagga cagtaaagtt ttatcccgct ctttcgtcgg
    15241 tattgtggtc cgcttgtagg cctgataagc gtagcgcatc aggcaatgct tcctgcttta
    15301 taagcccttc agcaactttt caacaaattc tggcaccacc tggcttgccg ggccgtaata
    15361 tttctcggca aattcattac caacctgact cggttcaaga ttcagttcaa cggtgtgagc
    15421 accatgtagt ttggcttcgt gtacaaaccc agccgccgga tagacatgcc cggaagtacc
    15481 aatggcaata aagacatccg ccatcgacaa cgccatataa atttcatcca tgccgagtgg
    15541 catttcgcca aaccaaacga cgtgcgggcg caagggtgcc gggaactggc aacaatggca
    15601 tttatcttct ggcgtaacgt ctccggtcca gtcgagaacc tgaccacttt gcgaacaacg
    15661 aactttcagc aattccccat gcatatgaat gacattggta ttacctgcgc gttcatgcag
    15721 gttgtctata ttctgcgtta ccagcaaaaa gcgatcgccg agagcgtctt gcagtttagc
    15781 cagcgcaaga tgcgcggcgt taggctgaat ttctggctgc tgcagctgtc gacgacgggc
    15841 gttataaaac gcttgcacca gttcaggatc gcgatcgaaa ccttccggcg ttgccacatc
    15901 ttcaacccga tgttcttccc acaggccatc tgcggcgcga aaggtacgaa tacctgattc
    15961 cgcagaaatt cctgcccctg tcagtacgag tactcttggt ttttccattg cttccggcac
    16021 cactttatct ctgaaaaaaa tacgctgacg caaacgctcg cgcaggcggc gtttattttt
    16081 acgaaaacga cttaaccgat gaccccgacg cgacagcata gcaacctctg ttgtttaatc
    16141 ggttagatgt aggaaggccg caccacgcat tcctcccgca tcaccgtggc gcgcgcgttc
    16201 aatgcgcgga acacgagcta caggtaagag atgacaaggc aacctgtccg ccagttgcgt
    16261 tgtgattgcc gggaaattcg ataagccacc accaatgacg accaggtcag ggtcaacaat
    16321 ggtcaggata tttcccagag aaaccgctaa taaatccaga taacgctcaa cgtgcgccct
    16381 tgcctgctca tcgccttgat cataaagcgc aataatttcg ggagcctgca acggttgatg
    16441 ataatagtgt tgatacagcc acgcaaaacc gcgaccagac agataatttt caatgcagcc
    16501 atgctgacca cagccgcagc ggcgtaacgg gaaatccaac cccatcatgg ttaacgcatc
    16561 aaccggcaga cgcatatggc caaactcgcc ggtaatgtag cttttgccag taatcggttt
    16621 gccgttgaaa atcagcccgc cgccaacgcc ggtgccgaga attaacccca tcaccagtgg
    16681 atattgcgta aattcatcat cccaggcttc tgaaagggca aaacagttgg catcgttatc
    16741 aaggcgtaca tcgcgatcaa gacgtgcgct caggtcggca cgcagcggtt tgccgctggc
    16801 agcggggaca ttagcggcat acagcttacc atcttctgtt tccggcattc ccggaatacc
    16861 gatgccgaca gaacctttac agccaaaacg ttggtcagct tcagctacca gctcacacac
    16921 tgcatctaaa aatgcgtcat agctgtcacg cggtgtcggc acccgctttt cccactgcaa
    16981 ctgccgaccg ctatcaaaca cgccaagcgc aatttttgtt ccaccaatat caaacccgta
    17041 atacattgcc gctccttgtt ttaatgtact gcctttactg gccgctaagg actcgcgcag
    17101 ggtcaatatt actggcgcgc cgcgccggat accaacttgc caaaagactc agcaacaatg
    17161 ctgtgaccag tacgtagaag acgtccagcc agtgcaattc cgatggcagg aagtcaataa
    17221 aatagatatc gctggagagg aactgatgac cgatcagctt ttcaatccac tcaataatcg
    17281 gggtaagttg cagtgaaaca actacgccga taaccacccc acacaggctg ccgaacagcc
    17341 ctgccagcaa tccataccag acaaagatgg cgcgaattaa accatctttc gcccccagcg
    17401 ttcttaatac tgcgatatcg ccactcttgt ctttcaccgc catcactaag gtggagacga
    17461 tgttgaaaca ggccacgcca atcaccagta ccatcgccag atacataatg gcgcggatca
    17521 tctggatatc gcgatacata tagccgtaag taccaatcca acttttaata taaacatagc
    17581 tgttggtcac ttcacccgca tcgcgtacca gcttattggc gttgaaaaca tccgtcattt
    17641 taagggcaat acctgacacg ctggaaccca tatcaagata ttgttgggca tccgccagcg
    17701 ggatcatggc aaaactgtga tcgagttgac cactcaactg caaaataccg gcaatgtgca
    17761 aacgcacacg ttttggctgc atcagtttat gctcgggatt cgagttgggg atcatgatcg
    17821 acacccaatc gccctgcttc actttcagcg catccgccac gcctttgccg atgataattt
    17881 gctgttcgcc cgctttaaaa ttgcgccacg catccccctg aacaaacgag ggtaatgcgc
    17941 tcagacgctg ttcctgttgt gggttaacgc ccttcacctg gattgcgcgc agattcgctc
    18001 cactttccac cagaccggtg aaattgatat acggcgcagc ggcggcaata cccggcactt
    18061 tctgcacgtt atccagtgct tcctgccagt tagtccatgg ctgatttacc gcttcgattt
    18121 cgccatgcgg caccaccgcc agaatgcggt tattcagttc gcgttcaaag ccgttcatcg
    18181 cgcttaagcc gacgatcaat accgccacgc cgagggcaat gccaatggta gaaatgacag
    18241 agattagcga caccatgccg ccgcgtcgcc gtccgcgact aaaacgcagg ccaatcaata
    18301 acgataaagg catcgccatt actccgcccc catcaggctc agttccgccg tcagacgtcc
    18361 atcacgcatt tccagttggc ggctcatacg tttcgccagt tgcaggtcgt gagtgaccac
    18421 caggaaagcg gtgccctgca agcgattcaa ctccccaagc aactggaaga tgctgtcggc
    18481 gttacgcgca tcgaggttac cggtaggttc atccgccagt accagacgcg gattattgac
    18541 cagcgcacgg gcaatcgcta cacgctggcg ttcgccgcca gaaagttcag atgggcggtg
    18601 attcgcacga tgctccagcc ccaccgcttt taacatctcc agtgcacggc tgttgatttc
    18661 agcgggcttt ttcttgccaa tcagcagtgg catagccacg ttttccaggg cagtaaaatc
    18721 cggcagcagg tggtgaaact gataaataaa gcccagcttc tggttgcgca gttctgcttt
    18781 cgccgctgaa gacagtttgc tcatcggctg accgttaaag atcacatcgc cggaggttgg
    18841 cgtatccagc ccgcctaaca ggtgcagcaa ggtactttta ccggaaccag agctaccgac
    18901 aattgccatc atttcacctt cgcccacgct gaaactgaca ttgtgcagca catcggtttg
    18961 cacactgcct tcctgatagc gtttgcacag gttgtcgcat tgcaacagga tcttattcat
    19021 aacgtaaagc ctcagcgggt tgtgtggcgg cagcgcgcca tgaagggtaa agcgtagaca
    19081 gcagcgcgat agccatcgcc accagcgcaa taacaatgac ctgtaaaggt tcgatagcca
    19141 ccggcagcgc cgcgccatca agcaggacgc cgattatcgg catcagatta tttaactggc
    19201 tggcaagcag tgcgccaagc gccgctccga ggattgcacc gataatcccg gcgctggccc
    19261 cttgcaccat aaagaccatc atgatttgtc gcggagttaa gccttgcgtt tgcaggatcg
    19321 cgacttcgcc ctgcttctcc atcaccatca gccccagcga ggtaataatg ttaaacgccg
    19381 caacagcgac aatcaggctc agcagtaagc ccatcatatt tttttccatg cgtacggcct
    19441 ggaacagctc gcctttacgg tcacgccagt cctgccattt gctgccttca ggcagttttt
    19501 gctgacttaa agagtcaact ttcagcggct catccagcca caaacgccag ccggtaatat
    19561 tggctgccgg ataacgcatc agacgcgagg catcctcaat attcaccagc atttcatagc
    19621 catcgacttc actgttggcg gcgaaagtgc caatcacatt gaacaggcgc tgacttggaa
    19681 tacgccccat cggcgtgaac tggctggcag atggcaccat cacgcggatt tgatcaccgc
    19741 gattgacgcc caactgtgag gcaagttgtt cgccgaggat gacattatat ttccccggct
    19801 cgaggtcagt ttgtttcaca ttgaccagat acggcgttag tggatctttt tgcaccgggt
    19861 cgataccgag catcaccccg accgccacgc tgcgcgcgct ttgcagtacc acatcaccgg
    19921 tagtaatagg tgcgacgcga ttaacgccgt ccagtttgac tgccgtttcc gggagttgct
    19981 gcgggttaag agagccatgc tcagaagaga gaattgcctg tggcatcagg ccaaggatgt
    20041 tgttttgcag ctcgcgctca aagccgttca tcactgacaa tactgtgacc agcgccatca
    20101 ccccgagggt aatgccgatg gtagaaagcc aggagacgaa acgaccgaag cgatccgctg
    20161 cacgcccacg catgtaacgc aggccaataa atagagcgac aggttggtac atgaaatccg
    20221 tctggttgct gtagcaaaga cacgagtata taagcgaaag caagatgggt aaacgtctga
    20281 actgtaagtg atcgtcaggg ctaatctgaa aagcccccgt aaatcaatcg gctaaattgc
    20341 catgttttat catcaatctt gccgcctgat cttaatctga aaaatcatcc aatacagaca
    20401 gatacccatt acacctcggg tcataaccag caggatcaac cgcatgtaaa ttaaaaaaga
    20461 gcgtgttgtc ctgatgaaac aaaaagagct atggattaac cagatcaaag ggttatgtat
    20521 ttgtctggtg gtgatttatc actcggtcat taccttttat ccgcatatga ccacttttca
    20581 gcatccgtta tcggaagtcc tgagcaaatg ctggatctat ttcaatcttt accttgcccc
    20641 ctttcgtatg ccggtttttt tctttatatc cggctatttg attcgccgct atatcgacag
    20701 cgtaccgtgg ggaaattgtc tcgataaacg gatctggaac atcttctggg tgctggcgct
    20761 ttggggcgtg gtgcagtggc tggcgttaag tgcgctgaac cagtggctgg cacctgagcg
    20821 cgatttaagc aatgcctcca atgccgctta tgccgattcc accggtgagt tcctgcacgg
    20881 gatgatcacc gccagcacca gcttatggta tctgtatgcg ttaattgtct atttcgtggt
    20941 atgtaaaatt tttagccgtc tggcgctgcc actattcgcc ttgtttgtac tgctgagtgt
    21001 ggcggttaat ttcgttccca cgccgtggtg gggaatgaac agtgtgatac gcaatttgcc
    21061 ttattacagc cttggcgcat ggtttggcgc aacaataatg acctgtgtta aagaggtgcc
    21121 gttgcgccgc catctgctga tggcttcttt gcttaccgtt ctggcggtcg gtgcctggtt
    21181 gtttactatc tcgctgctgt tgtcgctggt atcgattgtg gtgatcatga agctgtttta
    21241 tcagtacgag caacgattcg gtatgcgctc caccagcctg ctgaatgtga ttggctccaa
    21301 caccattgct atctacacca cccatcgcat tctggttgaa atattcagct taactctgct
    21361 tgcgcaaatg aacgcagcac gctggtcgcc gcaagtcgaa ctgacactcc tgctggttta
    21421 tccctttgtc agtttgttca tctgtactgt tgcgggcttg ctggtaagaa aactttcaca
    21481 gcgtgcattc agcgatctgt tgttctcccc gccttcactg cccgctgccg tcagttactc
    21541 tcgttaaacc atcgccccag ccggggcgat tcaatttgct aaaccatgtc gattacggat
    21601 aatgaacagc attgcttatc agagatatgc ccccatatgt tgaggcatat cctaacgaga
    21661 atctgacaac cgttatgcct gaacaatatc gttatacgct gcccgtcaaa gcgggtgagc
    21721 agcgtctgct gggcgagtta accggcgcag cctgtgcaac gctggtagcg gaaattgccg
    21781 aacgtcacgc cggtccggtg gtactcattg caccagatat gcaaaatgct ctgcgtttgc
    21841 atgatgaaat cagccagttc accgatcaga tggtgatgaa tctggcggac tgggaaactc
    21901 ttccctacga cagtttttcg cctcatcagg acattatctc ctcgcgcctt tccacccttt
    21961 accagctacc gacgatgcag cgtggcgtac tgattgttcc ggtgaatacg cttatgcagc
    22021 gcgtttgccc acacagtttt ctccacggtc atgcgctggt gatgaaaaaa ggtcagcgcc
    22081 tgtcacgaga tgcattacga acccaactgg acagcgccgg ttatcgccat gttgaccagg
    22141 tgatggagca cggcgaatac gccacgcgcg gcgcgttgct ggatctcttc ccgatgggga
    22201 gtgagctgcc ttatcgtctt gatttctttg atgatgaaat cgacagcctg cgggtgtttg
    22261 acgtcgacag ccagcgcacg ctggaggaag tagaagcgat caatctgctg cccgcgcacg
    22321 aatttccgac cgataaagcg gcaattgaac tgttccgcag ccagtggcgc gataccttcg
    22381 aagtgaagcg cgatccggaa catatttacc agcaagtgag taaaggcaca ttacctgccg
    22441 ggatcgagta ctggcagcca ttgttcttca gcgaaccact gccgccgctg ttcagttatt
    22501 tccctgccaa taccttgctc gtgaatactg gcgatctgga aaccagtgcc gaacgtttcc
    22561 aggctgacac gctggcgcgt tttgagaatc gcggcgtcga tccgatgcgc ccgctgttgc
    22621 caccacaatc gctctggctg cgggtggacg agctcttctc agagctgaaa aactggcccc
    22681 gagtgcagct aaaaactgaa catttaccga caaaagccgc gaatgccaat ttaggtttcc
    22741 agaaactgcc agacctggcc gttcaggcgc aacaaaaagc gccgctggat gcgctgcgta
    22801 agttcctcga gactttcgac ggtccggtgg tgttctcggt agaaagtgaa ggtcgccgtg
    22861 aagcgctggg tgaactgctc gcacgaatta aaattgctcc gcaacgcatt atgcgtcttg
    22921 atgaagccag cgaccgtggg cgttatctga tgattggcgc tgccgaacat ggttttgtcg
    22981 ataaggtgcg taatctggcg ctgatctgcg aaagcgatct gctcggtgaa cgtgttgccc
    23041 gccgtcgtca ggattctcgc cgcaccatca accccgatac actgatccgt aaccttgcgg
    23101 agctgcatat tggtcagccg gtggtccatc tggagcacgg cgtcggtcgc tatgccggaa
    23161 tgaccacgct ggaagctggc ggcattactg gcgagtacct gatgctcacc tatgccaacg
    23221 acgccaaact gtatgttccg gtgtcgtcac tgcatctgat tagccgttac gcaggtggcg
    23281 cggaagaaaa cgccccgctg cataaacttg gcggcgatgc gtggtcacgc gcgcggcaga
    23341 aagcggcgga aaaagtgcgt gatgtggcgg cggaattgct ggatatctac gcgcaacgcg
    23401 ccgccaaaga gggcttcgcg tttaaacacg atcgtgagca gtatcagttg ttctgcgaca
    23461 gcttcccgtt tgaaaccacg ccggatcagg cgcaggccat taatgcggta cttagcgaca
    23521 tgtgtcagcc gctggcaatg gatcgtctgg tgtgcggcga tgttggcttt ggtaaaacag
    23581 aagtggcgat gcgcgccgct ttcctggcag tagataacca caagcaggta gcggtgttgg
    23641 tgcctaccac ccttcttgcg cagcagcatt acgacaactt ccgcgaccgt ttcgccaact
    23701 ggccggtacg tatcgaaatg atctcccgtt tccgcagcgc caaagagcag acgcaaatcc
    23761 ttgcggaagt ggcggaaggg aaaatcgata ttctgatcgg tacgcacaaa ctgctgcaaa
    23821 gtgacgtcaa gtttaaagat ttaggcctgc tgattgtcga tgaagaacac cgcttcgggg
    23881 tgcgtcataa agagcgcatt aaagcgatgc gcgcgaacgt ggatattctg acgcttactg
    23941 caacaccgat cccacgtacg ctgaatatgg caatgagcgg aatgcgtgac ctgtcgatta
    24001 tcgccacgcc gcccgcccgt cgtctggcag ttaaaacctt tgtccgtgag tatgacagcc
    24061 tggtggtccg ggaggcgatc ctgcgtgaaa ttttgcgcgg aggacaggtt tattatctct
    24121 acaatgatgt ggaaaacatt cagaaagccg ccgaacggct ggcagaactg gtgcctgaag
    24181 cacggattgc catcggtcac gggcagatgc gcgagcgcga actggaacgg gtgatgaatg
    24241 atttccatca tcaacgtttc aacgtgctgg tttgtaccac cattatcgaa accgggatcg
    24301 acatcccgac agccaacacc attatcattg aacgtgcgga tcacttcggt ctggcgcagc
    24361 tgcaccagtt acgcggtcgc gtcggacgtt cacatcatca ggcatatgca tggctgctga
    24421 cgccgcatcc aaaagcgatg actaccgatg cacaaaaacg tcttgaagcg attgcctcgc
    24481 tggaagatct cggggcaggt tttgcgctgg caacgcacga tctggagatt cgcggcgcgg
    24541 gtgaactgct tggcgaagaa caaagcggct caatggaaac catcggtttc tcgctgtata
    24601 tggagttgct ggaaaacgcc gtcgatgcac tgaaagccgg acgcgagccg tcgctggaag
    24661 atctcaccag ccagcaaaca gaagtcgagc tgcggatgcc gtcgctattg ccagatgatt
    24721 tcatccctga cgtgaacacg cgtctgtcgt tctacaaacg tattgccagc gccaaaacgg
    24781 aaaacgaact ggaagagatc aaagtcgagc ttatcgatcg cttcggcctg ctgccagatc
    24841 cggcgcgtac cctgctggat attgcccgtc tgcgccagca agcgcagaaa ctggggatca
    24901 ggaagctgga aggtaatgag aaaggcgggg tgatcgaatt tgccgagaag aatcacgtta
    24961 atccggcctg gttgattggt ttgctgcaaa aacagccgca gcattaccgc cttgatggtc
    25021 cgacacgcct gaaatttatt caggatttga gtgagcggaa aacgcgtatc gaatgggtac
    25081 gccagtttat gcgtgaactg gaagagaacg cgatcgctta atgtaaatcg ggggcgtttc
    25141 tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt
    25201 caccatccat aattcaggct tatctgttta ttacaataac cttatattta ttatggattt
    25261 ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc
    25321 gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc gagcagtcgt
    25381 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    25441 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    25501 tacgtaccgc gcgcgggcag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
    25561 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    25621 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    25681 ccgacaatgg tgaccacagt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    25741 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    25801 ccggataacc caataggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    25861 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    25921 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    25981 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    26041 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    26101 agcgcaatgc aatcatttaa agatgcagca caaactgacg ctgaagtgat gcaacatgtg
    26161 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    26221 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    26281 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    26341 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    26401 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg atagtaccga
    26461 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagttg gcaaatgaca
    26521 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    26581 gcagatgaat gacgtcgcaa gaaattactt aacgatcgat aactaaatcg atagatgtga
    26641 tctggatcac atacaagata ttttttataa caatcattaa ttaatttaaa aatctttaaa
    26701 tttcagttaa atagaaagca attattttta tgggatctgg actggtggac agaggtgatt
    26761 atttttataa taacctttca ttcacagtca ccaggtacaa cggcatcatg gcaactgact
    26821 caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc
    26881 ttgataaggc catgaaattg ttctggcaac acg
//
LOCUS       DADLSE010000043        26624 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLSE010000043 DADLSE010000000
VERSION     DADLSE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.043"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..375)
                     /locus_tag="JN692_001815"
     CDS             complement(<1..375)
                     /locus_tag="JN692_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264142.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINKFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQ"
     gene            899..2827
                     /gene="thrS"
                     /locus_tag="JN692_001816"
     CDS             899..2827
                     /gene="thrS"
                     /locus_tag="JN692_001816"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7264143.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2831..3373
                     /gene="infC"
                     /locus_tag="JN692_001817"
     CDS             2831..3373
                     /gene="infC"
                     /locus_tag="JN692_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7264144.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3470..3667
                     /gene="rpmI"
                     /locus_tag="JN692_001818"
     CDS             3470..3667
                     /gene="rpmI"
                     /locus_tag="JN692_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7264145.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3720..4076
                     /gene="rplT"
                     /locus_tag="JN692_001819"
     CDS             3720..4076
                     /gene="rplT"
                     /locus_tag="JN692_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7264146.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            4199..4243
                     /locus_tag="JN692_001820"
     CDS             4199..4243
                     /locus_tag="JN692_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7264147.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4526..5509
                     /gene="pheS"
                     /locus_tag="JN692_001821"
     CDS             4526..5509
                     /gene="pheS"
                     /locus_tag="JN692_001821"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7264148.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5524..7911
                     /gene="pheT"
                     /locus_tag="JN692_001822"
     CDS             5524..7911
                     /gene="pheT"
                     /locus_tag="JN692_001822"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7264149.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQTSLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7916..8215
                     /gene="ihfA"
                     /locus_tag="JN692_001823"
     CDS             7916..8215
                     /gene="ihfA"
                     /locus_tag="JN692_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7264150.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8316..9296
                     /gene="btuC"
                     /locus_tag="JN692_001824"
     CDS             8316..9296
                     /gene="btuC"
                     /locus_tag="JN692_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7264151.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9359..9910
                     /gene="btuE"
                     /locus_tag="JN692_001825"
     CDS             9359..9910
                     /gene="btuE"
                     /locus_tag="JN692_001825"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7264152.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9910..10659
                     /gene="btuD"
                     /locus_tag="JN692_001826"
     CDS             9910..10659
                     /gene="btuD"
                     /locus_tag="JN692_001826"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7264153.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSVLDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10737..11201
                     /locus_tag="JN692_001827"
     CDS             10737..11201
                     /locus_tag="JN692_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7264154.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11448..12161
                     /gene="ydiV"
                     /locus_tag="JN692_001828"
     CDS             11448..12161
                     /gene="ydiV"
                     /locus_tag="JN692_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HAY7264155.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12225..13661
                     /locus_tag="JN692_001829"
     CDS             12225..13661
                     /locus_tag="JN692_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7264156.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN692_001830"
     CDS             complement(13665..13856)
                     /gene="hemP"
                     /locus_tag="JN692_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7264157.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN692_001831"
     CDS             complement(13988..15034)
                     /gene="aroH"
                     /locus_tag="JN692_001831"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7264158.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN692_001832"
     CDS             complement(15191..16024)
                     /gene="ppsR"
                     /locus_tag="JN692_001832"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7264159.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="JN692_001833"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="JN692_001833"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7264160.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN692_001834"
                     /pseudo
     CDS             complement(18792..20492)
                     /gene="fadK"
                     /locus_tag="JN692_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20495..20788)
                     /locus_tag="JN692_001835"
     CDS             complement(20495..20788)
                     /locus_tag="JN692_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7264161.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="JN692_001836"
     CDS             complement(20785..22074)
                     /locus_tag="JN692_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7264162.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="JN692_001837"
     CDS             complement(22130..23068)
                     /locus_tag="JN692_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7264163.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYDPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="JN692_001838"
     CDS             complement(23088..23852)
                     /locus_tag="JN692_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7264164.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24168..25079
                     /locus_tag="JN692_001839"
     CDS             24168..25079
                     /locus_tag="JN692_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7264165.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLINTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(25122..26273)
                     /locus_tag="JN692_001840"
     CDS             complement(25122..26273)
                     /locus_tag="JN692_001840"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7264166.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYSRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(26287..>26624)
                     /locus_tag="JN692_001841"
     CDS             complement(26287..>26624)
                     /locus_tag="JN692_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264167.1"
                     /translation="GSLKTEITDGKLNIVQEGRVKKFIRELPEITFSGKIALERGLDV
                     RYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPELKLMDERLFIDAAM
                     GFVLPEAAH"
BASE COUNT         6580 a         6438 c         6932 g         6674 t
ORIGIN      
        1 ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg tggtttccag
       61 taataaggaa atgatttctt ttttatagtg taatgtaatg agtatatttt ctgcatggat
      121 gggtgaagtg gcaaagttaa tggtaaaaag agcagtggaa taacaatttt gtgatatttt
      181 tcgacagttt gcggtaaatt cctgagtaac acagtatgac agcatcgaac ttaagtcaga
      241 ggtatcaagt agggtgatga gctggcgcgc aaatttatta ataggctctg atgaaggtgt
      301 tggtatgtcg ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat
      361 atcgttttga ctcataatct attcgcctta tatatatctg gaggttttga ggatgatgat
      421 taattattat cgccagaacg aaatgtgcac gaaaaattgt tataagaatt gtgtcacttt
      481 tatcattgtg atatgaccac ttcgtcatta atctcatgag cgtttaaaat actaccgcca
      541 aatcacgcaa cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa
      601 cctttcagac gcaccgtgat gttatcagtg gttctttaag cgttttgctg gtgtactcac
      661 tacaaacgaa ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta
      721 gtgttaacat cgttcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct
      781 gattgcgaac caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac
      841 aaatttttct ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat
      901 gcctgttata actcttcctg atggcagcca acgccattac gaccacgctg taagccccat
      961 ggatgttgcg ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa
     1021 tggcgaactg gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac
     1081 cgccaaagac gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca
     1141 tgcgattaaa caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg
     1201 tttttattac gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga
     1261 gaagcggatg catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg
     1321 gcacgaagcg cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga
     1381 cgaaaacatc gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat
     1441 gtgccgcggt ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac
     1501 ggcaggggct tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac
     1561 ggcgtgggca gacaaaaaag cacttaacgc ttacctgcag cgcctggaag aagccgcgaa
     1621 acgcgaccac cgtaaaatcg gtaaacagct cgacctgtac catatgcagg aagaagcgcc
     1681 gggtatggta ttctggcaca acgacggctg gaccatcttc cgtgaactgg aagtgtttgt
     1741 tcgttctaaa ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg
     1801 tgtcctgtgg gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacatc
     1861 ttctgagaac cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt
     1921 caaccagggg ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg
     1981 ccaccgtaac gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca
     2041 ggatgacgcg catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat
     2101 ccgtttagtc tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc
     2161 cactcgtcct gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct
     2221 ggcggttgcg ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt
     2281 ctacggtccg aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg
     2341 tacagtacag ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggcgaaga
     2401 caacgagcgt aaggtaccgg taatgattca ccgcgcaatt ctggggtcga tggagcgttt
     2461 catcggtatc ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccagttca
     2521 ggttgttatc atgaatatta ccgattcaca gtctgaatac gttaacgaat tgacgcaaaa
     2581 actatcaaat gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa
     2641 aatccgcgag cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt
     2701 ggaatcaggc aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt
     2761 aaatgaagtg atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga
     2821 ggaataaggt attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg
     2881 cgaaattcgc gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt
     2941 gagtctgaga gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag
     3001 ccctaacgcc gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa
     3061 gagcaagtct tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa
     3121 attccgtcct ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt
     3181 tctcgaagag ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca
     3241 ccagcaaatc ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcagt
     3301 agtcgaatcc ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa
     3361 gaagaaacag taaggccttc aagtagcact gtctgtggag cctttgggct tcacaggcat
     3421 tgttcgcctt tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat
     3481 taagaccgta cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca
     3541 caagcacgct aacctgcgtc acattctgac taaaaaagcg accaaacgta aacgtcacct
     3601 gcgtccgaaa gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta
     3661 cgcataagcc gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata
     3721 tggctcgcgt aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag
     3781 ctaaaggcta ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca
     3841 aagctggtca gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt
     3901 ggattgcgcg tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca
     3961 atggcctgaa aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat
     4021 tcgacaaagt agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca
     4081 gttgaaagag ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt
     4141 atcgccgtag ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat
     4201 gaatgctgct attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc
     4261 gtgagaagag aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg
     4321 cgcgttttga aaaataaaaa gccagtctat caggcgtaat tgtcgcagcc agtctggaca
     4381 cggccagtgc ggagaaaccg gagcgtactt aagtacgtga ggatttcgag cactgcccgg
     4441 gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt
     4501 caccactgac acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa
     4561 ggcggccatt agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt
     4621 gggtaaaaaa gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga
     4681 gcgtccggca gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa
     4741 tgcgcgtaaa gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat
     4801 tgatgtctct ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac
     4861 catcgaccgt atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga
     4921 aatcgaagac gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg
     4981 cgctgaccac gacactttct ggtttgacgc tacccgcctg ctgcgtaccc agacctctgg
     5041 cgtacagatc cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg
     5101 tgtttatcgt aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct
     5161 gattgttgat accaacatca gctttaccaa cctgaaaggc acgctgcacg acttcctgcg
     5221 taacttcttt gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgtttaccga
     5281 gccttctgca gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggctg
     5341 cgggatggtg catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg
     5401 tttcgccttc gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct
     5461 gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag
     5521 attatgaaat tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat
     5581 acgctggcga atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc
     5641 ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct
     5701 gacaaactgc gtgtgacaaa agtaaatgtc ggcggcgatc gcctgctgga tatcgtctgc
     5761 ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg
     5821 ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggcg aaccgtctga agggatgctg
     5881 tgctcctttt ctgagctggg aatttctgac gatcacagtg gcattatcga actgcctgcg
     5941 gatgcgccga ttggcaccga catccgcgaa tacctgaaac tcgatgataa caccatcgaa
     6001 atcagcgtga caccaaaccg tgccgactgc ttaggtatca ttggtgttgc gcgtgacgtt
     6061 gccgtgctga atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc
     6121 gacgacacgt tgccgattac agtcgaagcg ccggaagcct gcccgcgcta tcttggccgt
     6181 gtggtaaaag gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt
     6241 cgttgcggga tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa
     6301 ctgggccagc cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg
     6361 atggcaaaag agggcgaaac gctggtgctg cttgatggca ctgaagcgaa gctgaatgct
     6421 gacactctgg tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc
     6481 gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc
     6541 ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt
     6601 tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg
     6661 ctgatcgaca tctgcggtgg tgaggctggc ccggtaattg atatcaccaa cgaagcaacg
     6721 ctgccgaagc gtgcaaccat cactctacgt cgtagcaaac tggatcgcct gataggccat
     6781 catattgctg atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa
     6841 ggcaaagacg aatggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa
     6901 gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta
     6961 cagacaagcc tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa
     7021 acgctgctca acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa
     7081 gtgcagcaga tgatccatcc aggcgttgaa gccttactgc tggcaagccc gatctctgtt
     7141 gaaatgtcag caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac
     7201 cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat
     7261 actcaggcac cgttgggtat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac
     7321 cgttacgaag agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc
     7381 gaccttgaat ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa
     7441 gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt
     7501 ggttttgttg gagttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact
     7561 ctggtgttcg aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag
     7621 atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc
     7681 gcagcggata ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac
     7741 ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc
     7801 ctgatcctgc aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc
     7861 aaatgtgtag aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc
     7921 gcttacaaaa gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga
     7981 tgccaaagaa ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga
     8041 acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg
     8101 acgtaacccg aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag
     8161 acccgggcag aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga
     8221 tctaactaaa aaggccgcta tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa
     8281 aatcaacgcc atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac
     8341 agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct
     8401 taagcctttg cgccggtgaa caatggattt cgccaggtga ctggtttact cctcgtggcg
     8461 aactgttcgt ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg
     8521 cgctggctat atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg
     8581 gactacttgg cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc
     8641 aagggcaact ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca
     8701 ctttaatact cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg
     8761 gcgttgcatt agggattatc tgcagcgcac taatgacgtg ggctatctac ttttccacct
     8821 ctgttgattt acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc
     8881 ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca
     8941 ggccgatgaa tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt
     9001 ggttctggcg caatgtgctg gtggcagcga ccggctggat ggttggcgtc agtgtggcgc
     9061 tggcgggtgc tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt
     9121 taaccgatca tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc
     9181 tggccgatat tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca
     9241 ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt aaaagcagga cgttagccgc
     9301 aaaaagacgg tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat
     9361 gcaagattcc attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga
     9421 gaagtacgcc ggtaatgtgc tgttaattgt caatgtcgcc tcaaagtgtg gcttaacgcc
     9481 gcaatatgag cagttggaga atattcaaaa agcctgggcc gatcgaggtt ttgtggtgct
     9541 gggattcccg tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac
     9601 ttactgtacc accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg
     9661 cgaaggacgc catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc
     9721 ggaagagagc ggattctatg cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga
     9781 tgatatttta tggaattttg aaaaattcct ggttggcagg gacggaaaag tcatccagcg
     9841 tttttccccg gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt
     9901 ggcaaaataa tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg
     9961 ccgctttcag gcgaggttcg ggctggggaa atcctgcacc tggtggggcc gaatggcgcg
    10021 ggtaagagta ccttactggc gcgaatggcc ggaatgacca gcggtaaggg aagcattcag
    10081 ttcgcggggc aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat
    10141 ctttcacaac agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat
    10201 cagcacgata aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat
    10261 gacaaactcg gtcgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt
    10321 gctgcggtgg tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt
    10381 gatgagccga tgaacagtct tgatgttgcg caacaaagtg tgttagacaa aattctgagc
    10441 gcgctgtgtc agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacgttg
    10501 cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga
    10561 gaagaggtgc tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg
    10621 gatatcgaag gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat
    10681 cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc
    10741 gtttctgcct tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac
    10801 cgccgccaaa tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc
    10861 tacaaagctg gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact
    10921 gttcgggatt tgtggttgtg acgatgcgcg atcgtttcga tttgcatctg ccccgtgaaa
    10981 ccaaacaaca agcctctatt ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc
    11041 tggtcttttt caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca
    11101 acaaccaatt tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg
    11161 tctattggca gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaattgacgg
    11221 cgatgataca tcgccgtcaa tttactcctg aatacagtag tattttgcag acaaaaattc
    11281 cgtaaatgga ttattcgttt ataatcagga taatctaaaa aaaggccccc tgaaaaagtg
    11341 gttaacatgg aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc
    11401 ccttttagcc ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt
    11461 tggagaatct ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc
    11521 ttgtcggcgt tgagttaatt acccatttct ccagtgaaga tggcacggtt cgtatcccga
    11581 cgagtcgggt tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt
    11641 tggaattact aaaatcatgc caacactttt ttattcaaca taaactcttt gcctggctta
    11701 atttaacgcc acaagtggct acattattat tagacaggga taattacgca ggcgaactat
    11761 taaaatatcc gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa
    11821 aagataatcg cgatcttttg tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg
    11881 gagcaggtaa tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtaatgctgg
    11941 agaaaggttt tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga
    12001 tccaggcgca aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgccag
    12061 aaatattggc acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg
    12121 ccgttcctat aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt
    12181 ttaagacgag agtaaccgtc tacactatca aacagatagg atctatgacc ctgtctttta
    12241 ttacccgctg gcgcgatgaa ttgccagaaa cctatacagc actttcccct acgcctttaa
    12301 ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt attccatcgt
    12361 cgctgtttaa aaatgccgca ggtgtctggg gcggcgaaac cctactgcct ggtatgtcac
    12421 cactggcaca ggtttacagt ggtcatcagt tcggcgtctg ggcgggccaa ctgggtgatg
    12481 ggcgcggcat tttacttggc gaacaactgc ttgctgatgg cactacaatg gactggcatc
    12541 tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg gttttacgtt
    12601 cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt ccgacgaccc
    12661 gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg gagccaggcg
    12721 cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa catttttact
    12781 atcgccgcga gccggaaaag gttcgtcagt tggctgactt tgccattcgt cattactggt
    12841 cacatcttga agacgatgag gacaaatacc gtctctggtt taacgatgtt gtcgcacgta
    12901 ccgcgtcgtt aattgcccaa tggcagacgg tcggctttgc acatggggtg atgaataccg
    12961 acaacatgtc gctgctggga ctgacgcttg attacgggcc gtttggtttt cttgatgatt
    13021 acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttatagc tttgataatc
    13081 aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca ccatttgttg
    13141 ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg acgcattatg
    13201 gacaacggat gcggcagaaa ctgggcttca tgacggagca aaaagaggat aacgcgctac
    13261 tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc acattccgca
    13321 tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag tttattgatc
    13381 gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa gacgaggtta
    13441 gcgatagtga gcgtcagcaa ctgatgcaaa gcgttaaccc agctctggta ttgcgcaact
    13501 ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgatggaa ttgcaccgcc
    13561 tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc agtcgtccac
    13621 ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt agttaacagc
    13681 agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc gataatcagt
    13741 ttgccatccg gacctaacag tgtctggctg gtaattcgcc ggatgggctg cggggaagcg
    13801 gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata acgcataaaa
    13861 attaataata attgatataa caatgattct tattatcaat attatggcag gccagagcaa
    13921 gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat caggaatggg
    13981 cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg cgttcggtat
    14041 cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc tgaccgccga
    14101 cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca atctccgtag
    14161 agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga cggtgctgct
    14221 tctggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca aactcgtgca
    14281 gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg ccgccacgca
    14341 taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga ccatttttgt
    14401 ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccacg gcaatccgcg
    14461 tgttgccatc ggtaccattt ttaaaaccta ccggacagga gagtgctgaa gccatttcgc
    14521 ggtggatctg actttcggta gtacgagcgc caatcgcgcc ccaactgatt aaatcagcaa
    14581 taaactgacc ggtcaccata tcgaggaact cagtcgcggt agggacaccc agctcattta
    14641 cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag ctgccgttta
    14701 aatccggatc ggagattagc cctttccagc cgacaacagt tcgtggtttt tcaaaatagg
    14761 tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc agacgggtgg
    14821 cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg accaacagtc
    14881 gcttatcttc accattcagt attttttcaa ttctgcggcg ggagtcggtg acatgggtgg
    14941 cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc aggctctcaa
    15001 tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat ttgttgcttc
    15061 tacggcagga ctaccggaaa gtgatgcgca tcacgataaa ccaatcgcag ctgatttcaa
    15121 gtgcgaggtg tgtcacacct cgccgggagg ttagcatgat aacaaaaaaa caaaccaatg
    15181 cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc ttggtggcaa
    15241 tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta cggtacaagg
    15301 cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga ctgttctccc
    15361 gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg aacaatttat
    15421 gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg gcaataaagg
    15481 ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg ggggttttac
    15541 cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc aacgaaatgc
    15601 cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat ttattgagat
    15661 tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt ttcatctctt
    15721 gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaagccttca ctttgcaaga
    15781 tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc acgcctgtct
    15841 ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca ttttcgacaa
    15901 acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg tgtcctaata
    15961 cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca acagcattat
    16021 ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt aaaaacgcac
    16081 agaagcgtag gacgtaatgt ctggtttata aaatgaacct tcaattttat tttttatgaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggcgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag atatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttacct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccaccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata tattcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aatcatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gctaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatggataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaatgccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 cggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ccgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggatcata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atggtttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcggggtttt
    23941 cgacggagca agctgttttc cttgccaaca atcttgcaaa aaaaatgcga tggctaaaac
    24001 aaaagtgcaa tttgcggcaa tatagttatt ccatgaccgt acaatcttgc attcagtgta
    24061 aataatcttg tttagctctc gctaaacgcg gttgtataac cgcaatacac gccgtatttt
    24121 atccggcata tattgttttt gcttgctcat ggaataggga ggttgtcatg tatcaacgct
    24181 gttttgataa tgccagtgaa acgctgtttg tcgccggtaa aacgccacgg ctttcacgtt
    24241 ttgcatttag cgatgatcca aaatgggagt ctggacatca cgttcatgac aatgaaaccg
    24301 agctgattta cgtcaagaaa ggggttgcaa ggtttaccat cgattcttcg ttatatgtcg
    24361 cacatgcgga tgacattgtg gtgatagaac gcggcaggct gcatgcggtg gcctctgacg
    24421 ttaacgatcc ggcaacgacg tgtacctgtg cgctgtacgg ctttcagttt cagggggctg
    24481 aggaaaatca gttactgcaa ccgcattctt gtccggtaat tgccgcgggg cagggaaaag
    24541 aagtcataaa aaccttattt aatgagctaa gtgtgatttt gccgcaaagt aaaaatagcc
    24601 aaacatcttc gttatgggat gcatttgcct atacattagc aattctttac tacgaaaact
    24661 ttaaaaatgc ttatcgttcg gagcaggggt atattaaaaa agatgttctg ataaaagata
    24721 ttcttttcta tctgaataat aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa
    24781 aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc
    24841 ctattaacta tgttattcaa cggcgtatga cggaagcaaa atggtcactc attaatactg
    24901 aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttgcca
    24961 aactgttttt gcgccatgtc ggttgttcgc ccagcgatta ccgtaggcaa tttaaaaatt
    25021 gttttgcgga acaagaaatc ttatctgaat ttcctcaacc ggtaagtctt gccggataaa
    25081 tctggcggcg cactttgtta aaaagctgcg ccgcctgcag attatttgtt ctgatagtct
    25141 ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt
    25201 tcacaacgga catcacgcca gaagcgggag acgcgcgcct catcggtata gcccagaccg
    25261 cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcaca gtacagtttt
    25321 gccagcgccg cgctggtgcg cagtgattga tgctgatcgg cttgccatgc cactttcagc
    25381 accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc
    25441 tggttatgcc caatgggttt accaaaagcg atacgctggt tggcatagcg agcggcatct
    25501 tcaaaggcac actcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca
    25561 aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca
    25621 acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc
    25681 agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt
    25741 ttgggatctt tcggttgcgg atcgcgcgcc aacaccagca tatacgggta ctctttcgca
    25801 ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg
    25861 gtagtggcac tgttgttgtc tgagcctgcg cctggctccg tcaacgccag ggcataggcg
    25921 ggatcacccg tttccagtgt gctttctgcc gttttacgta gctgctctgc agaaccgaaa
    25981 cggcgcatac tgtgaataca ttgaccgttg gtaatcaaaa atgccggagc accgcatttt
    26041 gacacttcca tcagcgccag catctgggta acgtaatccg cagggatacc gccgaactct
    26101 tccggcacac caagcatgga aataccgtta tccgccagtg cccgcataaa ctcacgcggg
    26161 tatgttccgt tttgatcgca ggtgcgggaa tactcttccg gaaagttagt cgtaatcaac
    26221 tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc tatcgtgata
    26281 ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct
    26341 ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat
    26401 atctttttgt aaatcgacgc caggagcgat ttcgattaaa tgcaggccgt cttctttcag
    26461 cgtgaataca gcacgttcgg tgatgtaacg aacatccagt ccccgttcga gcgcgatttt
    26521 tccgctgaaa gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac
    26581 gatatttaat ttgccgtcgg taatttctgt ttttaaactg cccg
//
LOCUS       DADLSE010000044        26311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLSE010000044 DADLSE010000000
VERSION     DADLSE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.044"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1371
                     /locus_tag="JN692_001842"
     CDS             <1..1371
                     /locus_tag="JN692_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7264168.1"
                     /translation="DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQ
                     EARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTEL
                     AKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNE
                     AELLGYGQGERLRECMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYL
                     SERLKDQETFYAESDAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNI
                     TEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIRTAANSMSGAGGFIGSIGSWLGG
                     AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLG
                     VRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRD
                     GGMLSG"
     gene            1364..1705
                     /locus_tag="JN692_001843"
     CDS             1364..1705
                     /locus_tag="JN692_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264169.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            1705..2403
                     /locus_tag="JN692_001844"
     CDS             1705..2403
                     /locus_tag="JN692_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7264170.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQSGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYMGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            2414..3157
                     /locus_tag="JN692_001845"
     CDS             2414..3157
                     /locus_tag="JN692_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264171.1"
                     /translation="MTQTESAILAHTRRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMVVT
                     GFYRVPLSSAQPGDILLCCFGASVANHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWNTSAFTGIYNDLAAASACM"
     gene            3055..3735
                     /locus_tag="JN692_001846"
     CDS             3055..3735
                     /locus_tag="JN692_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HAY7264172.1"
                     /translation="MATTNAFCLASPPLEHICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRLQMNEGWYQIRIAGEDTAPEVVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSAMAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            3976..7444
                     /locus_tag="JN692_001847"
                     /pseudo
     CDS             3976..7444
                     /locus_tag="JN692_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            7513..8136
                     /locus_tag="JN692_001848"
     CDS             7513..8136
                     /locus_tag="JN692_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7264173.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTVHLGGIVALSYASVKKSDTMKTGENTFHYESLHG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVEMVVIDLAYEGSGSGDWRTDGFIVGIGYKF"
     gene            8201..11014
                     /locus_tag="JN692_001849"
     CDS             8201..11014
                     /locus_tag="JN692_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264174.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSASEAATRATDAADSARAANTSAG
                     QAATSAQEAFSSAGTASAKASEASKSAAAAESSKSAAATSAGAAKTSETNAAASVTAA
                     EKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETRAESSKTAAAS
                     SASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAA
                     TRAETAAKRAEDIASAVALEDANTTKKGIVQLSSATNSTSETLAATPKAVKAANDNAN
                     GRVPSNRKVNGKALTADITLTPKDIGTLNSVTISFSGGAGWFKLATVTMPQASSIVYI
                     ALIGGAGYNVVSPQQAGISELVLRAGNGNPKGITGALWKRTAVGLTNFAWINTSGDTY
                     DIYVEIGNYATSVNIHWDCTTNASVSIYTSPTYSASKPSSVTDGVVYTMYSSHQKPTP
                     SDIGALPTTGGTISGPLSVTDGITGALKGNADTATKLAAAPKINGVKFDGSADINLTP
                     ENIGAFARRSTGAYADSDGAVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKA
                     HYRNGGLFYRSSRDGYGFEEDRAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQG
                     QTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASST
                     DLGTKTTSSFDYGTKSSNATGDHNHSRGTMEITGTLGYFRSDSGNFYTASEAFTLGGS
                     APAHGFTGSQFTYGVPVNFNASRTWSGATSTTGNHAHSVPIGAHAHSVAIGSHGHTIT
                     VNAAGNAENTVKNIAFNYIVRLA"
     gene            11014..11598
                     /locus_tag="JN692_001850"
     CDS             11014..11598
                     /locus_tag="JN692_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7264175.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPLDIPAGFVAVFNSDESSWHLFEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIRDAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(11714..11962)
                     /locus_tag="JN692_001851"
     CDS             complement(11714..11962)
                     /locus_tag="JN692_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7264176.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADAWFVH"
     gene            complement(12024..13121)
                     /locus_tag="JN692_001852"
                     /pseudo
     CDS             complement(12024..13121)
                     /locus_tag="JN692_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13210..14247
                     /gene="dusA"
                     /locus_tag="JN692_001853"
     CDS             13210..14247
                     /gene="dusA"
                     /locus_tag="JN692_001853"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7264177.1"
                     /translation="MHDNHETQKINQTSVMPEKTGVYWNSRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFLGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            14381..14623
                     /gene="pspG"
                     /locus_tag="JN692_001854"
     CDS             14381..14623
                     /gene="pspG"
                     /locus_tag="JN692_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7264178.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(14789..15772)
                     /locus_tag="JN692_001855"
     CDS             complement(14789..15772)
                     /locus_tag="JN692_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7264179.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            15855..17270
                     /locus_tag="JN692_001856"
     CDS             15855..17270
                     /locus_tag="JN692_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7264180.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            17323..18402
                     /gene="alr"
                     /locus_tag="JN692_001857"
     CDS             17323..18402
                     /gene="alr"
                     /locus_tag="JN692_001857"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7264181.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRGAMKYVD"
     gene            18655..19848
                     /gene="tyrB"
                     /locus_tag="JN692_001858"
     CDS             18655..19848
                     /gene="tyrB"
                     /locus_tag="JN692_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7264182.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(20071..20613)
                     /locus_tag="JN692_001859"
     CDS             complement(20071..20613)
                     /locus_tag="JN692_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264183.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPREEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            20976..21689
                     /locus_tag="JN692_001860"
     CDS             20976..21689
                     /locus_tag="JN692_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7264184.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            21800..22216
                     /locus_tag="JN692_001861"
     CDS             21800..22216
                     /locus_tag="JN692_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7264185.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            22220..22576
                     /locus_tag="JN692_001862"
     CDS             22220..22576
                     /locus_tag="JN692_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7264186.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN692_001863"
     CDS             complement(22611..25433)
                     /gene="uvrA"
                     /locus_tag="JN692_001863"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7264187.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTSGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            25687..26223
                     /gene="ssb1"
                     /locus_tag="JN692_001864"
     CDS             25687..26223
                     /gene="ssb1"
                     /locus_tag="JN692_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7264188.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
BASE COUNT         6381 a         6467 c         7282 g         6181 t
ORIGIN      
        1 gataaacagc agagtgccct gacaaaaaca gagcgtgagc tggccctgtc cagactgaaa
       61 ggggaagaaa aagagcgtgt ccgactgggg tatgcggcgg atgacctcgg ttttgtgggt
      121 gatccgtatc aggaggcgag acaacgttat atcagtaatg ccctggaagc ctggcgcaat
      181 aacgaggcga ataaacccaa atcccggggt ggaaaatcag agacggaaaa agcggaagac
      241 agtttttccc ggctgctgaa gcagcagaaa gagcaattgg cacttgcggg gcagaataca
      301 gagctggcga agctgaaata ccagactgcg cagggcgaac tgaaaaccct gacggagatg
      361 cagaagcagg aactgctgcg caatgcggcc ctgattgacc agcaaaaaat ccgggagcag
      421 ttgcggtccc gggaagagac cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa
      481 gcggaactgc tggggtacgg gcagggagag cggctccgtg aatgcatgcg ggagttgcag
      541 cagatccgcg acagtttccg ccagaaggat gcggaccttc agtctcagta tcagaccggg
      601 gatatcagtg aggattttta cagacaggct ctggcacaga acgcgcagta tctgagtgaa
      661 cgtcttaaag accaggaaac tttttatgcc gaatcggatg cgcagcgtgc ggactggcag
      721 aaagggctgc aggaggggtt cagtaactgg gtggataatg cgtccgatta cgcctcacag
      781 gcagcacagc ttgcgacgga gggtatctca gggatggtga ataacatcac ggagatgctg
      841 aacggaaata aagtggaatg gcgcagctgg gcctcatcag tgctgcagga aatatcaaaa
      901 gttcttatga atgccgcgat tgtcaacggg atcaggacgg cggcaaacag tatgtccgga
      961 gcgggaggat ttatcggcag tattggtagc tggctgggcg gtgcggtggc caatgcaaaa
     1021 ggcggcgtgt atacctcggc aaacctgagt gcgtacagca acagtattgt ggatacgccc
     1081 acgtactttg cctttgcaaa aggggcggga ctgatggggg aggccggtcc tgaagccatt
     1141 atgcccctga cccgggcggc ggatggctcg ctgggtgtgc gagcggtggg cagtatgaac
     1201 ggcagtgcag gtctggtgta ttccccggtc taccatatcg ccattcagaa tgacgggact
     1261 aatggccaga tagggccgga agctgcgggc agccttgtgc aactgattga ccagcgggtg
     1321 caggcggtga tgctgtccat gcgacgtgac ggaggaatgc tgagtggctg agataaaaac
     1381 gctgcatctg gtcccgcgtg aagggatgca ggtgagtgag aaaccgtcgg tggcgagggt
     1441 acggtttggt gacggttatg aacagcgccg cccgacggga cttaatgccc gactgaagac
     1501 gtttcaggcg gtgttccggg tgacggatga ggcgacccgg cgatggctgg aagagttttt
     1561 atcgtggcat ggtggttacc gtgccttttt gtggcgaccg ccgaaacata accggacggt
     1621 gagggtggta tgccgggagt ggagcgtcac agataacgcc aggtacagtg atttcagttg
     1681 tacgattgag caggtggtga actgatgcag gatattcacg aagaaagtct gaacgagtcg
     1741 gttaaatcag agcagtcacc gcgggtggta ctctgggaaa tcgacctgac ggtacagagc
     1801 ggtgagcggt attttttctg taatgagctg aatgaaaaag gggagccggt cacctggcag
     1861 gggcgtaagt atgaggcgta cccgattgat ggcagcggct ttgaaatgaa cgggaaaggc
     1921 agcagtgccc gcccgtcgct gacggtgtcc aatctgtttg gccttgtcac cgggatggcg
     1981 gaggatttgc agagtctggt gggtgccacg gtggtccgtc gccgggtgta tgcgcgtttt
     2041 ctggatgcgg tgaattttgt ggcgggcaat ccggaagcgg acccggaaca ggaactgagc
     2101 gaccgctggg tggtggagca gttatcagag ctgacggcca tgacggcctc gtttgtgctg
     2161 gcgacaccga cggagacgga cggggcgctg tttcccggtc gcatcatgct ggcgaacacc
     2221 tgtatgtgga cctaccgctc tgatgagtgt ggttacatgg gcggggctgt ggcggatgag
     2281 ttcgataaac ccaccacgga tatccgtaag gacagatgca gcaagtgcat gcgcgggtgt
     2341 gaactgcgca ggaatgtcgg caattttggc ggtttccttt ccattaataa actttcgcag
     2401 taaatcccgg tttatgacac agactgaatc agcgattctg gcacacaccc ggcgatgtgc
     2461 gcctgcggag tcttgcggct tcgtgataag cacgccggag ggggagcggt atctgccgtg
     2521 tgtgaatatc tccgcagagc cggaggcgta ttttcgtatc gcaccggaag actggctgcg
     2581 ggcagagatg cagggggaga ttgtggcact ggtccacagt catcccggtg agctgccctg
     2641 gctgagcgag gctgaccggc ggctgcagat aaaaagcgca ctgccctggt ggctggtctg
     2701 ccggggggaa attcataaat tccgctgtgt gccacatctg acaggacggc gctttgagca
     2761 cggggtgacg gactgttaca cgctgttccg ggatgcatac catctggcgg gaattgatat
     2821 gccggatttt catcgcgagg atgactggtg gcgcaacggc cagaacctgt acctggacaa
     2881 tatggtggtc accggctttt accgggtgcc cctgtcctct gcacagccgg gcgatatcct
     2941 gctgtgctgc ttcggcgcat cggtggctaa tcatgccgcc atttactgcg gcaacggtga
     3001 actgcttcac catattcctg aacaactgag taaacgggag aggtattccg aaaaatggca
     3061 acgacgaacg cattctgtct ggcgtcaccg ccactggaac acatctgcct tcacggggat
     3121 ttacaacgat ttggccgccg cctcagcctg tatgtgaaca cggcagcgga agccattcgc
     3181 gccctgtcga tgcagatgcc gggctttcgc cttcagatga acgaaggctg gtaccagata
     3241 cgtattgccg gtgaagacac ggcaccggag gtggtgtacg cccgccttca cgaacagctg
     3301 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
     3361 cagattgtgt tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcaatg
     3421 gcgttatggg gttcagccat ggcagccggt ggtttttctg ccaccacgat gctgttttca
     3481 cttggagcca gcatgattct gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca
     3541 ccggattacc gcgcaacgga taacggcaga cagaacacgt acttttcctc gctggataac
     3601 atgattgccc aggggaaccc gatgccggtg ccttacgggg aaatgctggt tggctcccgc
     3661 cgtatatccc aggacatcag cacccgtgat gaaggcgggg gcggaaaggt cgtggttatc
     3721 gggcggcagg gataaaacat aaaaaaatcc cgcagtgatc gcggagctgc ggggacagac
     3781 aaatgaagat caatgttaag gagttgtttt tgttactcgg gcaaaaaaac actaacgcag
     3841 cgaaattata agcgccacag tcagtgtgtg aaaatgtgaa gatattcaga aattttattc
     3901 cgtcatgacg caggcacccg gtgaggtgcc tgttgttttt gtgagtgaac aattatcacg
     3961 gtaagaggtg atgtaatggg caaaggtggc ggcagggcgc acacaccgcg tgaggcgaaa
     4021 gacaatctca aatccacgca gatgatgagc gtgattgatg cgattggtga gggaccgata
     4081 gaaggcccgg tgaaaggcct gcagagtatt ctggtgaaca aaaccccgct gacggacacg
     4141 gacggtaatc ccgtgataca cggtgtgacc gcggtctggc gtgccgggga gcaggagcag
     4201 acaccaccgg aaggctttga gtcctctggc tctgaaactg tactgggtgt cgaagtgacc
     4261 agggcaaaac cggtaacacg caccattacg tcagcgaaca ttgaccgcct gcgggtgacc
     4321 ttcggggtgc agtcactggt ggagaccacg tcaaagggtg accgtaatcc gtcctctgtc
     4381 cgtctgctga ttcagttaca gcgtaacggt aactgggtga cggaaaagga tgtcaccatt
     4441 aacggcaaga ccacctcaca gttccttgcg tcggtgattc tggataatct gcctccccgc
     4501 ccctttaaca tccggatggt cagggagacg gcggacagca ccacggacca gctgcagaac
     4561 agaacgctgt ggtcgtcata caccgaaatc atcgatgtga aacagtgcta cccgaacacg
     4621 gccattgtgg ggatgcaggt ggatgcggag cagtttggtg gtcagcagat gacggtgaac
     4681 taccatatcc gcggtcgcat catccaggtg ccgtcaaact atgacccgga aaaacgcacg
     4741 tacagtggta tctgggacgg cagtctgaaa ccggcataca gcaacaatcc ggcctggtgt
     4801 ctgtgggaca tgctgactca cccgcgctac ggcatgggaa aacgtctggg ggcggcggat
     4861 gtggacaagt gggcgctgta tgccatcggg cagtactgcg accagatggt gccggatggc
     4921 ttcgggggca ccgagccgcg gatgaccttc aatgcgtacc tggcacaaca gcgtaaggcg
     4981 tgggatgttc tcagtgattt ctgctcggcg atgcgatgta tgccggtatg gaacggtcag
     5041 acgctgacgt tcgttcagga ccgcccgtcg gatgtggtgt ggccgtacac cagcagcgat
     5101 gtggtggtgg atgataacgg cgtggggttc cgctacagct tcagtgccct gaaggaccgg
     5161 cacacggcgg tggaggtgaa ttacaccgac ccgcagaacg gctggcagac ctccacggaa
     5221 ctggtggaag acccggaagc catactgcgc tacgggcgca acctgctgaa gatggatgcg
     5281 ttcggctgca ccagtcgcgg tcaggcccac cgtgccggac tgtgggtgat aaagaccgga
     5341 ctgctggaaa cgcagacggt ggatttcacg ctcgggtcac aggggctgcg tcacacaccc
     5401 ggtgacatta ttgaaatctg tgataacgac tatgccggga ccatgaccgg cggacgtgtc
     5461 ctgtccatcg atgccgccac ccgcaccctg acgctggacc gtgaggtgac actgccggag
     5521 accggtgccg ccacggtgaa cctgattaac ggcagcggta agccggtgag tgtggacatc
     5581 accgcacacc ccgcgccgga ccggatacag gtcagtaccc tgccggatgg cgtggagaca
     5641 tacggtgtgt ggggactctc cctgccgtca ttgcgccgtc gcctgttccg ctgtgtctcc
     5701 gtccgggaaa acacggacgg cacctttgcc atcacggcag tgcagcatgt gccggagaaa
     5761 gaagccatcg tggataacgg ggcgcacttt gacggcgacc agagcgagcg tcatccctcc
     5821 ggcagtgcag cacctgacgg tggaggtgag cgcagctgac ggccagtatc tggcgcaggt
     5881 gaaatgggac acgccgcggg tggtgaaggg cgtgcgcttc agtctgcgac tgaccagcgg
     5941 aagcggagaa ggcagccatc tggtgaccac cgccatcact gcggatacag agcatcgttt
     6001 cagtggtctg ccgctcgggg aatacaccct gacagtcagg gcaattaaca gttatggcca
     6061 gcagggcgaa ccggccacca ccaccttccg gattaacgcg ccagcaaaac ccgccaccat
     6121 tgagctgacg ccggggtatt ttcagataac ggcggtccca cgtcttgcgg tgtatgaccc
     6181 gacggtacag tttgaattct ggttctcaga aaaacgcatc acgaacacag cacaggtgga
     6241 aaaatctgcc cgttatctgg ggaccggcag tcagtggact gtctccggcc cgcacattaa
     6301 gcccgggaag gatttctggt tttatgtacg cagcgttaac ctagtgggga aatctgcttt
     6361 tgttgaagcc agtggacggg ccagcaatga tgcagaaggg tatctggggc tgtttcggga
     6421 aaaaatagga aaactgcatc tggctcaggg gctgtgggag ctgatagaca acagccagct
     6481 tgcggatgag atggcggaga tgaagaccac catcaccgaa acccgcaatg aaatcacaca
     6541 gacggtcagt aaaacgctgg aagaccagag cgccaccatt cagcagatac agcgcgtgca
     6601 gaaggacaca aatgatgacc tggctgcgct gtacatgctg aaggttcaaa aaacgaaaga
     6661 cggcattccc tatgtggccg ggattggtgc agggattgag gatactgatg gccagccact
     6721 gagcaacata ctgctgctgg ctgaccgtat cgcgatgata aatccggaga acggcaacag
     6781 cacgccgtta tttgtggcgc aggggaatca gctgttcatg aacgacgtgt tcctgaagcg
     6841 actgtttgcg gtgagtatca cgtcatccgg caaccccccg acgttttccc tgacgccgga
     6901 agggaagctg acagccagga acgcggatat cagcggagca attaccgcga ataccggcac
     6961 gctcaataat gtcaccatta acgagaactg tgtcatcaga gggaaactgt ctgcaaacca
     7021 gattgaaggc gacctggtga agacggtggg gaaagccttt ccccggaata acagttatgc
     7081 cagcgggacg gtaactgtca cagtttacga tgaccaggga ttcgaccggc agattatcat
     7141 tcccccggtg ctgtttcgcg gggcgaaaca ccagaatttc aacagcccga atcagcagtc
     7201 gtactggtat tccacctgta agctgcaggt gctgaagaac ggggttgaga ttttccatga
     7261 accggcaacg gatgtcagcc gggtgttctc atcggtgata gatatgccgg cagggcaggg
     7321 tcatgtcacc ctgacgttta atgtgtcgtc ggccggtgcg aacaactgga cgccgacaac
     7381 gtacatcagt gatttactgg ttgtggtcat gaaaaaatcc acggcaggga tcagtatcag
     7441 ctgacggttt attaacccgg acgggcacct caggaggtgc cttttttatt gactgaaaac
     7501 aaagaggtaa tcatgcggca tttatacgca acgatattat tgtttactac cctgctggca
     7561 ggaattgcct ttcctgcaca ggctgaaagc ggacacggtg cattttccgt gggatatgct
     7621 caggttcacc cgggcggcgt accggcattg tccggtaccg gtgcgcgtgc aggtgattta
     7681 aaagggatta atgtgaaata ccgttatgag ttcacggttc acctgggcgg cattgtcgcg
     7741 ctgagttatg catcggtgaa gaaaagtgac acgatgaaga cgggtgaaaa taccttccat
     7801 tatgaaagcc tgcacggtcg ttatgtcagt ctgatggccg gccctgtctg gcagctcagt
     7861 gagcgggtca gtctctatgg catggccggg atggcgtaca cccgctggtc tgacagtgtt
     7921 caggattacc ggcgtgatga agtaaaaccg gggtatgtga aggagaccac caccgccagt
     7981 gatggtcata ctgcgcgtca tctgtcgccg gcctggaatg ccgggattca gttcagtccc
     8041 gtagagatgg tggttattga ccttgcttat gaaggttccg gcagtggcga ctggcgcact
     8101 gacggtttca tcgtgggtat cggttataaa ttctgattag ccaggtaaca cagtgttatg
     8161 acagcccgcc ggttcaggcg ggcttttttg tggggtgaat atggcagtaa agatttcagg
     8221 tgtactgaaa gacggcacag gaaaaccggt acagaactgc acaatccagc tgaaagcaaa
     8281 acgtaacagc accacggtgg tggtgaacac gctggcatct gaaaatccgg atgaagccgg
     8341 gcgttacagc atggacgttg agtacggtca gtacagcgtt attctgttgg tggaagggtt
     8401 cccgccgtca catgccggga ccatcaccgt gtatgaagat tctcaacccg gtacgctgaa
     8461 tgattttctt ggtgccatga ctgaggatga tgtccgtccg gaggcactgc gccgttttga
     8521 actgatggtg gaagaggtgg cgcgtaacgc gtccgcagta gcacagaaca cggcagccgc
     8581 gaagaagtca gccggcgatg ccggcacatc tgccagtgag gcggcaaccc gtgcgactga
     8641 tgctgcagat tcagcacgtg cagccaacac gtcagccgga caggccgcga cgtcggctca
     8701 ggaggctttt tccagcgcag gaacggcatc agcaaaagcc tctgaggcat cgaaaagtgc
     8761 tgctgctgca gagtcctcaa aaagcgcggc ggccaccagt gccggtgcag cgaaaacgtc
     8821 agaaacgaat gcggcagcca gcgttaccgc cgctgaaaaa tcggcagaaa gcgccgcatc
     8881 gtccgcttca acagccacaa cgaaggctgg cgaagccact gaacaggcca gcgcagcagc
     8941 gaggtctgct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc
     9001 agaatcctca aaaacggctg ccgcatcatc cgccagttcg gcggcgtcat cggcatcatc
     9061 tgcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg gcagcgccac
     9121 gacggcatcc acaaaggcga cagaggctgc tggcagtgcg acagcggcag cacagagcaa
     9181 aagtacggca gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg cagaggatat
     9241 tgcatccgcc gtggcgcttg aggatgcgaa cacgacgaaa aaggggatag tccagctcag
     9301 tagtgcaacc aacagcacat ctgaaacact tgccgcgaca ccgaaggcag ttaaagcagc
     9361 gaatgacaat gcgaatggca gggtaccatc taaccgaaaa gttaacggga aagcactgac
     9421 tgcggatata acattaacac cgaaagatat tggcacttta aattcagtaa cgatctcttt
     9481 ctctggcggg gcagggtggt tcaaactggc aacggttacc atgccacaag cgagttccat
     9541 cgtttacatc gcattgattg gtggcgctgg ttacaacgtc gtctccccac agcaggcagg
     9601 catttcagaa ctggttctac gagcaggcaa tggaaacccc aaagggatta ccggagcttt
     9661 gtggaagcgt acagccgtcg gattaacgaa tttcgcctgg atcaacacat ccggcgatac
     9721 atatgatatt tacgttgaga ttggcaatta tgcgacgagt gtaaatatcc attgggattg
     9781 tactacaaat gcgtcagttt ctatttatac ctcgccaaca tattcagcga gtaagccttc
     9841 cagcgttacc gatggtgttg tttatacgat gtatagctca catcagaaac ctacaccatc
     9901 agatattgga gcgctgccaa cgactggagg gactatttca ggtccgttgt ctgttactga
     9961 tgggatcacc ggggcactga aggggaacgc cgataccgcg acgaaacttg cggcagcccc
    10021 aaaaattaac ggtgttaagt ttgatggctc ggcggatatt aacctcacgc cggaaaatat
    10081 tggtgcattt gcccgacgtt cgacgggggc ttatgcggat tcggatggag ccgttccctg
    10141 gaatgccgaa tcaggcgctt acaatgtcac ccgctctggc gacagctata ttctggttaa
    10201 cttctatacc ggagtcggaa gttgccggac cttgcagatg aaggcgcatt acagaaatgg
    10261 tggtctgttc taccgttctt caagagacgg ttatggtttt gaggaagaca gggcagaagt
    10321 ttatacctcg aaaaatcttc caccagaaag ctacccagtc ggcgcaccaa tcccgtggcc
    10381 atcagatacc gttccgtctg gttatgccct gatgcagggg cagacttttg acaaatctgc
    10441 ctacccgaaa cttgcagccg cttatccgtc aggcgtgatc cctgatatgc gtggctggac
    10501 gattaagggc aaacctgcca gtggtcgtgc cgtattgtct caggaacagg acggcattaa
    10561 atcgcacacc cacagcgcca gtgcatccag tacggatttg gggacgaaaa ccacgtcgtc
    10621 gtttgattat ggtactaagt cttcaaatgc cactggtgac cataaccaca gccgaggcac
    10681 tatggaaatt accggtactc tcggttactt cagaagtgat agcggtaatt tctatacagc
    10741 aagtgaagca tttacactgg gtggctctgc gccagcccat ggatttacgg gctctcaatt
    10801 tacttatggt gttcctgtaa actttaacgc ttccagaacc tggtctggtg ccacaagcac
    10861 tacaggtaac catgcgcact ctgttccaat tggtgctcat gcgcactccg ttgcgattgg
    10921 ttcacatgga cacaccatca ccgttaacgc tgctggtaac gcggaaaaca ccgtcaaaaa
    10981 catcgcattt aactatattg tgaggcttgc ataatggcat tcagaatgag tgaacaacca
    11041 cggaccataa aaatttataa tctgctggcc ggaactaatg aatttattgg tgaaggtgac
    11101 gcatatattc cgcctcatac aggtctgcct gcaaacagta ccgatattgc accgttagat
    11161 attccggcag gcttcgtggc tgttttcaac agtgatgagt catcgtggca tctctttgaa
    11221 gaccatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt tatttctgaa
    11281 ctcggtccgt taccggaaaa tgttacctgg ttatcgccgg aaggggagtt tcagaagtgg
    11341 aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat ccgggatgcg
    11401 gaagaaacaa aaaacaacct gatgcaggta gccagtgagc atattgcgcc gcttcaggat
    11461 gctgcagatc tggaaattgc aacggaggaa gaaacctcat tgctggaagc ctggaaaaag
    11521 tatcgggtgt tgctgaaccg tgttgataca tcaactgcac ctgatattga gtggcctacg
    11581 aaccctgtca gggagtaatc attgggatta tgccgcagca cgtcttaagc aagaacgtgc
    11641 tgcggttgga tggtgaactt tcgatagtgc gagtattgaa tgatttccag ccgttaccga
    11701 ttttacgtgt taattagtga acaaaccacg cgtcagcaga ctcccaggta tctttcagag
    11761 tttcctgaac aaaagtttta gctgaatctt tatcggcggt gcgcgtaaca gaaaggccat
    11821 cgttgctggt agcttttacg atcacctcta catcgtcata acgtttactg atgcgtcggg
    11881 ttaattcttc ctttaacgca tccacagcac cggttggcat tttagtcatt ttttctttgg
    11941 ctatgcagat ttcaatacgc ataaaagtcc ctctatactg tgtttgtata cagtattatt
    12001 tttaactgta tggataaaca gtgtcaagag atcttatttc tgctcctttg gagctcttca
    12061 aaacgattat gtaaagattt cggatacagt tcggtatata cctgccatag cacgtttaat
    12121 gaacgatgcc ctgtaacctg ggctacttcc tcaatactaa aaccagcctc aaataagcga
    12181 cttgcccctt ctctacgcaa atcatggtat cgcagatcct taatacctaa tttgcttctt
    12241 accctctgaa atcccgcagt aacagaagtg ctgttatatg gaaaaatgaa ttccgatttt
    12301 tggggctgtc gttggacgat atcccaggct tccccaagca aggctacttt catgtggttg
    12361 ccttcctttt tgcgtggatc tttcctgtct cttacgagta tagatttttg ttcctggtcg
    12421 agatcttctc atcgtaaccg gcatacttct ccgattcgca tacaggacca cacagaaaat
    12481 ttgaggatat caacgaacgg aatttttgag catttatgag tagatcgttg ttgaaggcct
    12541 tcaatgagca tgtccagttc atcagatgct ggtctacgat tacgacggtt tgatttacca
    12601 atcaaaccaa gtttaagtag gtatggacga gcacttttcg ccgggtttga tgtgtaatta
    12661 attccgtata caggtttggc cgcatccaga acactgccaa gataactaac atcgtggctg
    12721 actgttgctg gacttgcgcc agcgttgttt cttagcctgc aatgttcaat tacgtcattt
    12781 tctgtcagtt cagatagttt gatcgcggag atgtcactat ccataagcag ttccagcaca
    12841 tatcttttag tacggcctgc tttacctccg gcatttgggt catttaaata tttgtgtagt
    12901 aagtcacgga ctgtaagtcc gtcaactgca tttgatgatg gaattccata tagatctaat
    12961 tccatcactt tctgtgtgcc ccatgttttg gcatgagcat gtttagggaa tgttttgctt
    13021 tccctgtaag tgataacacc tttttctttg ataatcacat tacagcgata gcgtggtgtg
    13081 ccatcggatt ttagtcgttt ctctatgtta tagtacgcca ttacacgacc tcgttatttc
    13141 gggttcccat aaaacgtggg aacctgtgcg ggaacctgac gcgagaaaaa tagcctgaaa
    13201 tgttcaaaaa tgcacgataa tcatgaaaca caaaaaatta atcaaaccag cgtgatgcct
    13261 gaaaaaactg gtgtttactg gaattctcgg tttagcgttg caccaatgct cgactggacg
    13321 gacagacatt gccgctattt cttgcgtctg ctttcccgca atacgttgct ttataccgaa
    13381 atggtgacca caggggcgat tattcacggt aaaggtgatt atctggcgta cagtgaagaa
    13441 gaacatccgg tagcgttgca actgggcggt agcgatccgg cggcgctggc gcagtgtgca
    13501 aagctggcag aagcgcgcgg atatgatgag atcaacctga atgtcggctg cccgtctgac
    13561 cgggtgcaga acggcatgtt tggtgcgtgt ctgatgggta atgcgcagct ggttgccgac
    13621 tgcgtgaaag cgatgcgcga tgtggtgtcg attccggtga cggtgaaaac gcgtattggc
    13681 atcgacgacc aggacagcta tgaatttctc tgcgatttca tcaataccgt ttccggcaaa
    13741 ggcgagtgtg agatgttcat catccacgca cgtaaagcct ggctttcggg gttaagcccg
    13801 aaagaaaacc gtgaaatccc gccgctcgat tatccgcgtg tgtatcaact gaagcgtgac
    13861 tttccgcatc tgacaatgtc gattaacggt ggtatcaagt cgctggaaga agctaaagcg
    13921 catttgcaac atatggatgg cgtgatggtc gggcgcgagg cgtatcagaa tccgggtatt
    13981 ctggcggcgg tagaccggga gatctttggt tcctcggata ccgatgccga tccggtggca
    14041 gtagtgcgtg ccatgtatcc gtacattgag catgaactca gccaggggac gtatctcggc
    14101 catattatcc ggcatatgct gggcttgttc ctgggtattc ctggcgcgcg gcagtggcgg
    14161 cgttatttaa gtgaaaatgc ccataaagcg ggtgctgaca ttaacgtgct ggaacacgcg
    14221 ctcaaactgg tggcggataa gcgttaactt ttcaccaaaa agtagtcaaa ttcaccacgc
    14281 cctgcgcacc gtcgcggggc gttttgctgt taaatcaata gattattttt ggcatgattc
    14341 ttgtaatgcc agcaagagat ttcatatttg ggagagcatc atgctggaac tactttttgt
    14401 gattggcttt tttgtcatgc tgatggtcac cggcgtttcg ttgctgggca ttatcgccgc
    14461 gctggttgtg gcgacggcca ttatgttcct cggcggtatg ctggcattga tgattaagtt
    14521 gctgccgtgg ttactactgg cgattgcggt ggtgtgggtt attaaggcga ttaaagcacc
    14581 aaaaatgccg aaatatcagc gttatgaccg ctggcgttac taagggattg tgcggatgat
    14641 cacaacctaa ggttttatcc ttagaacaaa ataggaattg ataatcaaat ctgtcactat
    14701 tgcgcctcta acagattcat cgtgctgtac cctacataca gccgaactat aaaaagaaag
    14761 ggcttcccag gtggaagccc tatttctttt atggaatcag caggctggaa ccttgcgtcg
    14821 cccggctttc cagaatctca tgcgcacgct gcgcatcctt cagcggatat ttctgctgct
    14881 cggcgacatc gaccttaatc acaccgctgg caatcaaaga gaacagttca ttactggcct
    14941 cggttaattc ctcccgcgtg gtgatatagc cttgcaggga agggcgtgtc acatacaacg
    15001 agcctttttg attgagaatg cctaagttca caccggtaac cgcacctgat gagttgccaa
    15061 aactgaccat taagccgcgg cgttgcaggc aatccagcga ccgttcccag gtgtctctgc
    15121 ccacggaatc gtacactacg cgcactttct taccgccggt gatctctttc aatcgctcga
    15181 ccagattctc ttcacgatag ttaataacct gccacgcgcc ggcttttagc gcgctctgcg
    15241 ctttttgcgc ggttcctacg gtgccgatga gtttcgcgcc cagggctttt gcccactggc
    15301 aggcaattaa gccaacgccg ccagccgctg cgtggaacag gaactgttca tcgggtttaa
    15361 tttcataggt tttgcgcagc agataataaa ccgttaagcc tttcaggaag gatgccgcag
    15421 cttgctcaaa agaaattgcc gcaggcagaa tcgccgcttt atccgcatta atgttatgca
    15481 cagagctgta agcgcctaac gccgactgcg catagactac atgatcgcca gccttaatat
    15541 gctttacacc actgccgact ttactcacga tgcctgccgc ttcggtacct aatccgctgg
    15601 gtagcgatgg cggcgggtaa aggccgctgc ggatatacgt gtcgataaaa ttgatgccga
    15661 tggctttatt ttcgacctgg atttcattct ccgccggatc ggcaggagtg aactctacgg
    15721 cttgaagtac ttccgggcca ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc
    15781 cagaacgtat cgtcagggtc tgcttcatat ggtaaagttt cgacccattc tttatctcgg
    15841 taactccatt cactatggca ggaaataaac ccttcaacaa acagcaggct gaaccccgcg
    15901 aacgcgatcc acaagttgcc gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt
    15961 cggtgttggg cggtttaatg ctggataacg aacgctggga tgatgtagcc gagcgtgtgg
    16021 tagcagacga tttttacacc cgcccacacc gtcatatctt tactgaaatg gcgcgtttgc
    16081 aggaaagcgg tagtcctatc gatctgatta cccttgcgga atcgttggaa cgccaggggc
    16141 aactcgatag cgtcggtggt tttgcttatc tggcagagct gtcaaaaaat acgccaagtg
    16201 cggcgaacat cagtgcttat gcggacatcg tgcgtgaacg tgccgttgtc cgtgagatga
    16261 tctcggttgc gaatgagatt gctgaagccg gttttgatcc gcaggggcgt accagcgaag
    16321 atctgctgga ccttgctgaa tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag
    16381 acgaagggcc gaagaacatc gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt
    16441 tgtttcagca gccacacgat ggcgttaccg gggtaaacac cggttatgac gatctcaaca
    16501 aaaaaaccgc tggcttgcag ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg
    16561 gtaaaacaac atttgcgatg aacctcgtcg aaaacgcggc aatgttgcag gataaaccag
    16621 tacttatctt ctcgctggag atgccatcag aacagatcat gatgcgttct ctggcgtcgc
    16681 tgtcgcgcgt tgaccagact aaaatccgta ccgggcagct cgatgatgaa gactgggcgc
    16741 gcatttccgg caccatgggt attttgctcg aaaaacgcaa tatctatatc gatgactctt
    16801 ccggcctgac gccaacggaa gtgcgttccc gcgcacgccg tattgcccgt gaacacggcg
    16861 gcatcgggct tatcatgatc gactacctgc aactgatgcg cgtaccggcg ctttccgata
    16921 accgtacgct ggaaattgca gaaatctccc gctcgctgaa agcactggcg aaagaactga
    16981 acgtgccggt ggtggcgctg tcccagttga accgttctct ggaacaacgt gccgacaaac
    17041 gcccggtcaa ctccgacctg cgtgaatctg gctctatcga gcaggatgcg gacttgatca
    17101 tgtttatcta tcgtgatgag gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa
    17161 ttattatcgg taaacaacgt aacggcccaa tcgggacggt acgcctgacc tttaacggtc
    17221 aatggtcgcg cttcgacaac tatgcggggc cgcagtacga cgacgaataa taattatttt
    17281 atgaattagg taattaaagc aaacacttat caaggaacac aaatgcaagc ggcaactgtt
    17341 gtgattaacc gccgcgctct gcgacacaac ctgcaacgtc ttcgtgaact ggcacctgcc
    17401 agtaaaatgg ttgcggtggt gaaagcgaac gcttatggtc acggtcttct tgagaccgcg
    17461 cgaacgctcc ccgatgctga cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg
    17521 cgtgcggggg gaatcaccaa acctgtactg ttactcgaag gcttttttga tgccagagat
    17581 ctgccgacga tttctgcgca acattttcat accgccgtgc ataacgaaga acagctggct
    17641 gcgctggaag aggctagcct ggacgagccg gttaccgtct ggatgaaact cgataccggt
    17701 atgcaccgtc tgggcgtaag gccggaacag gctgaggcgt tttatcatcg cctgacccag
    17761 tgcaaaaacg ttcgtcagcc ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca
    17821 aaatgtggcg caaccgagaa acaactcgct atctttaata ccttttgcga aggcaaacct
    17881 ggtcaacgtt ccattgccgc gtcgggtggc attctgctgt ggccacagtc gcattttgac
    17941 tgggtgcgcc cgggcatcat tctttatggc gtctcgccgc tggaagatcg ctccaccggt
    18001 gccgattttg gctgtcagcc agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag
    18061 cataaagccg gagagcctgt tggttatggt ggaacctggg taagcgaacg tgatacccgt
    18121 cttggcgtag tcgcgatggg ctatggcgat ggttatccgc gcgccgcgcc gtccggtacg
    18181 ccagtgctgg tgaacggtcg cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc
    18241 tgcgtagact taggtccaca ggcgcaggac aaagccgggg atccggtcat tttatggggc
    18301 gaaggtttgc ccgtagaacg tatcgctgaa atgacgaaag taagcgctta cgaacttatt
    18361 acgcgcctga cttcaagggg cgcgatgaaa tacgtggatt aatcgttctg taatatttga
    18421 ttgtctgtgc cggatgcggc gtgaatgcct tatccggcca ataaaatcct aaaaattcaa
    18481 taagttgatg ttctttcatg ctcttataaa ggtcgtgcct ctggcggatg tacgtttatc
    18541 attagtctca ctctgttgct aattgccgtt cgctcctgaa catccactcg atcttcgcct
    18601 tcttccggtt tattgtgttt taaccacctg cccgtaaacc tggagaacca tcgcgtgttt
    18661 caaaaagttg acgcctacgc tggcgacccg attcttacgc ttatggagcg ttttaaagaa
    18721 gaccctcgca gcgacaaagt gaatttaagt atcggtctgt actacaacga agacggaatt
    18781 attccacaat tgaaagccgt ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc
    18841 gcttcgcttt atttaccgat ggaagggctt aacagctatc gccatgccat tgcgccgctg
    18901 ctgtttggtg cggaccatcc ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt
    18961 ggcggctcag gggcattgaa agtgggcgcg gatttcctga aacgctactt cccggaatca
    19021 ggcgtctggg tcagcgatcc tacttgggaa aaccacgtag caatattcgc cggggctgga
    19081 ttcgaagtga gtacttaccc ctggtatgac gaagtgacta acagcgtgcg ctttaatgac
    19141 ctgttggcga cgctgaaaac attacctgcc cgcagtattg tgttgctgca tccatgttgc
    19201 cacaacccaa cgggtgccga tctcactaat gatcagtggg atgcggtgat tgaaattctc
    19261 aaagcccgcg agcttatccc attcctcgat attgcctatc aaggatttag tgccggtatg
    19321 gaagaggatg cctacgctat tcgcgccatt gccagcgctg gattacccgc tctggtgagc
    19381 aattcgttct cgaaaatttt ctccctttac ggcgagcgcg tcggcggact ttctgttctg
    19441 tgtgaagatg ccgaagccgc tggccgcgta ctggggcaat tgaaagcaac agttcgccgc
    19501 aactactcca gcccgccgaa ttttggtgcg caggtggtgg ctgcagtgct gaatgacgag
    19561 gcattgaaag ccagctggct ggcggaagta gaagagatgc gtactcgcat tctggcaatg
    19621 cgtcaggaat tggtgaaggt attaagcaca gagatgccag aacgcaattt cgattatctg
    19681 cttaatcagc gcggcatgtt cagttatacc ggtttaagtg ccgctcaggt tgaccgacta
    19741 cgtgaagaat ttggtgtcta tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat
    19801 acggcaaatg tacaacgtgt ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca
    19861 ggctggagtt acccagcctg cagtgaaatt aaactgtcgt cgctttcact atttctttgt
    19921 agatgatttt tttgatgccg ttgttctacg tgagagataa taaaacgttg tcagttcttt
    19981 tattgttaag tttatcccaa ttatctggaa ttcctttccc tattttttcg tgtggagtaa
    20041 taatttctgc acatctaacc attattaaat ttaataataa gtatcgggtt tgttacgata
    20101 gaatatttct atatcctcaa gaatatgctt tgccttattg cctgtgactt tctgagattc
    20161 aattatagta tcgttaaatt ctaatgttaa agagaactct ttttttccgc tttgtgtaag
    20221 tttaacctta tattgattac caatatcaaa taaaaaggtc ctgtaatgag aataatcacc
    20281 tttaacctcc tcggcaaaac agcaaagcgt gcaaatatac ataaagtctt tcttacccct
    20341 gattttgtta actaaatcaa taaatgcgtg aaaaacttta cttgcaatag aacttgatac
    20401 ttcttctcta ggtctacaaa aatcttttgc aatttttaac tgtgtttcta tgtataaaaa
    20461 gaaatctttg gcataagaga aatctttggc ataagagata tctttattat ttagtgcatc
    20521 tagctcaaca ggtaaagatg tcgttttgtt gctattcaca tatgatatat tcatatcatc
    20581 atctccatcg cgagtaataa aattaatcac cattgtaggg tagggggctg gtcaatcaga
    20641 aatcatcttt ataaacttcg attgtttttg taatgctgta tcattaagtt cattaaatcg
    20701 tacagcagat aaatgttcta tcaaatttcg ctcatttgcc gaggattcat cataataaac
    20761 gtaaaattaa tgtatcctta catcgagtaa taaacatttt ttatacaaaa aaagacagga
    20821 acgtatttac tgggttaaat ataatcatcc tgcttttcat cacaaaaacc gcagataatc
    20881 cttcctttcc ccggcagctg gcgttatggt cagatggttt ttgcaacaaa tctcacaata
    20941 aaaagtttca acatactgac tatttaggga aaaatatgcg caagatcaca caggcaatca
    21001 gtgccgtttg cttattgttc gctctaaaca gttccgctgt tgccctggcc tcatctcctt
    21061 caccgcttaa ccctgggact aacgttgcca ggcttgctga acaggcacca attcattggg
    21121 tttcggtcgc acaaattgaa aatagcctcg cagggcgtcc gccaatggcg gtggggtttg
    21181 atatcgatga cacggtactt ttttccagtc cggggttctg gcgcggcaaa aaaaccttct
    21241 cgccagaaag cgaagattat ctgaaaaatc ctgtgttctg ggaaaaaatg aataatggct
    21301 gggatgaatt cagcattcca aaagaggtcg ctcgccagct gattgatatg catgtacgcc
    21361 gcggtgacgc gatcttcttt gtgactggtc gtagcccgac gaaaacagaa acggtttcaa
    21421 aaacgctggc ggataatttt catattcctg ccaccaacat gaatccggta atctttgcgg
    21481 gcgataaacc agggcaaaat acaaaatcgc aatggctgca ggataaaaat atccgaattt
    21541 tttatggcga ttccgataac gatattaccg ccgcacgcga tgtcggcgct cgtggtatcc
    21601 gcattctgcg cgcctccaac tctacctaca aacccttgcc acaagcgggt gcgtttggtg
    21661 aagaggtgat cgtcaattca gaatactgac agagcgggag agagtgatgc tctcccgaat
    21721 gctgtttttt taatcacacc tttatccttt cgctgtcttg ctgcaaactg attaagagag
    21781 ttttatcaag gagcagcaca tgtggtatca aaagacgctc acgcttagcg ccaaatctcg
    21841 tgggtttcat ctggtaacgg atgaaattct gaatcagctg gctgatatgc cgcgcgttaa
    21901 catcggctta ctgcatctgt tgctgcaaca tacctccgcc tctctgacac ttaatgagaa
    21961 ctgcgatccc accgtacgcc acgacatgga gtgttttttc ctccgcaccg ttcccgacaa
    22021 cggaaattat gagcatgact atgagggagc agacgatatg ccttctcata tcaaatcctc
    22081 aatgctggga acatcgcttg tattgccggt acataaaggg cgtattcaga ccggcacttg
    22141 gcaaggcatc tggctgggag aacatcgcat ccacggcgga tcgcgtcgca tcatcgcgac
    22201 actacaaggg gagtaaaaaa tgaccatttc ggagttgcta caatattgca tggcaaaacc
    22261 aggcgcagaa cagagcgtgc ataacgactg gaaagcgacg cagatcaaag tggaagatgt
    22321 actgtttgcg atggtgaaag aagtagaaaa tcgcccagct gtttcgctga aaaccagccc
    22381 ggagctggcg gagctgctac gtcagcagca cagcgatgtg cgtcctagcc gccatctgaa
    22441 taaagcgcac tggagcaccg tgtatctcga cggttcgctg ccggattcgc aaatctatta
    22501 tctggtggat gcgtcttatc agcaggcagt gaatttacta ccggaagaaa agcgtaaatt
    22561 gctggtacaa ctctgaaagg aaaaggccgc tcagaaagcg gccttaacga ttacagcatc
    22621 ggcttgagga agcgtgccgt atgcgaagct tcgcactccg cgacggtttc tggcgtaccg
    22681 gagacgagga tttcgccgcc gccactgccg ccttctggtc ccaggtcgac aatccagtca
    22741 gcggttttga tcacgtcgag attgtgctca atcaccacaa tggtattgcc ctgatcgcgc
    22801 agtttatgca gcacgtcgag gagttgctga atatcggcga agtgcagacc gctggtcggc
    22861 tcgtcgagaa tatacagcgt ctgcccggtg ccgcgttttg acagctcacg cgccagcttc
    22921 acgcgctggg cttcaccacc agaaagtgtg gttgcggact gccccaggcg aatgtacgtc
    22981 agaccaacgt ccatcaacgt ttgcagctta cgcgccaggg ctggcaccgc atcaaagaac
    23041 tcacgcgcct cttcgatggt catatccagc acttcgtgga tggttttgcc tttgtactta
    23101 atttccagcg tttcacggtt atagcgttta cctttgcact gatcgcacgg cacgtagata
    23161 tccggcagga agtgcatctc cactttgatc acaccgtcgc cctgacaggc ttcgcagcgc
    23221 ccgccacgga cgttaaagct gaaacgtcct ggcgtataac cacgcgcacg ggattccggt
    23281 acgcccgcaa acagttcgcg cacaggcgta aacacgccgg tataggtcgc cgggttagaa
    23341 cgtggagtac gaccaattgg gctttggtcg atatcgatca ctttatcgaa atgctccagt
    23401 ccctgaatat cgcgatacgg tgccggttcg gcgatggtcg caccattcaa ctggcgttgg
    23461 gcaatcggga acagtgtgtc gttaatcagc gtcgatttac cggaacctga aacccctgtg
    23521 atgcaggtaa acagaccgac tggcagcgtg agcgtcacgt ccttcaggtt gttgccgcgt
    23581 gcgcctgtca gcttcagcac tttttctgga ttcgccggaa cgcgtttctt cggcacttca
    23641 atcttgcgtt taccgctcat gtactgcccg gtcaacgatt caggcaccgc cataatcgct
    23701 tccagcggac cttctgcgac cacttcaccg ccgtgaacac ccgcaccagg accgatatcg
    23761 atcacatggt cagcggcgcg aattgcgtct tcgtcatgct ccaccacaat cacggtatta
    23821 ccgagatcgc gcagatggat aagcgtaccc aacaggcgct cgttatcgcg ctggtgcagg
    23881 ccgatagacg gctcgtccag cacgtacata acgccaacca ggcccgcacc aatctggctc
    23941 gccagacgga tacgctgggc ttcaccgccg gaaagtgttt ctgccgagcg ggaaagtgtc
    24001 aggtaattca ggccgacgtt tacgaggaat ttcaggcgat cgccgatctc tttaagaatt
    24061 ttttccgcaa tcttcgcccg ctgaccagcg agtttgagat tgttgaagaa ttccatcgca
    24121 tgaccgatgc tcatgtcgga gatagcaggc agcggcgtat tctcgacata aacgtggcgc
    24181 gcttcccgac gcagacgcgt cccttcgcag ctggcgcatg ggcgattgct gataaacttg
    24241 gctaattctt cacgtaccgc actggattct gtctctttat aacggcgctc catattgtgc
    24301 agcacgcctt cgaacggatg acgacggatg gaggtatcgc cacgatcgtt catgtatttg
    24361 aattcaatgt tttctttgcc agaaccgtac aacaccactt tatgcacgtt cgcgctcagg
    24421 ctgccccacg gcgcttcgac gtcgaactta tagtgatctg ccagcgattt cagcatctgg
    24481 aagtaataga agttgcggcg atcccagcca cggatcgcac cgccagccag tgacagctcg
    24541 gggttttgga tcacgcggtc aggatcgaaa tattgctgta cgccaaggcc gtcacaggtc
    24601 gggcaggcac ctgccgggtt gttaaacgaa aacagtcgcg gctccagttc acgcatactg
    24661 tagccgcaaa ttgggcaggc gaagttggcg gagaacagca gctcttccgc tttcgggtcg
    24721 tccatatccg ccactaccgc tgtaccaccg gaaagttcca gcgcggtttc aaacgactcc
    24781 gcaagacgtt gggtaagatc gtcacgcacc ttgaagcgat caaccaccac ttcaatggta
    24841 tgtttctttt gcagttccag tttcggcgga tcggaaagat cgcagacttc gccatcaata
    24901 cgagcacgga tgtaaccctg gcttgccagg ttctccagcg ttttggtgtg ttcgcctttg
    24961 cgctctttaa tgattggcgc aagcagcatc agacgcttgc cttccggctg cgacagcacg
    25021 ttatccacca tctggctgac ggtttgcgcc gccagcggga cgtcgtggtc cggacagcgc
    25081 ggttcgccga cgcgggcgaa caacaaacgc aaatagtcgt ggatttcggt gattgtcccc
    25141 accgtagaac gcgggttatg agacgtcgat ttctgctcaa ttgagatggc aggagaaagc
    25201 ccctcaatat gatcgacgtc cggcttttcc atcagtgaca gaaactgccg cgcgtaggcg
    25261 gaaagggatt caacgtaacg gcgctgccct tcggcatata aggtgtcgaa agcgagcgag
    25321 gatttgccag aacccgaaag cccggtcacg acaatgagct tgtcgcgggg aataacgagg
    25381 ttgatgtttt tgagattatg ggtgcgggcg ccccgaactt cgatcttatc cattcacctt
    25441 tcccggatta aacgcttttt tgcccggtgg catggtgcta ccggcgatca caaacggtta
    25501 attatgacac aaattgacct gaatgaatat acagtattgg aatgcattat ccggagtgtt
    25561 gtgtaacaat gtctggccag gtttgtttcc cggaaccgag gtcacaacat agtaaaagcg
    25621 ctattggtaa tggtacaatc gcgcgtttac acttattcag aacgattttt ttcaggagac
    25681 acgaacatgg ccagcagagg cgtaaacaag gttattctcg ttggtaatct gggtcaggac
    25741 ccggaagtac gctacatgcc aaatggtggc gcagttgcca acattacgct ggctacttcc
    25801 gaatcctggc gtgataaagc gaccggcgag atgaaagaac agactgaatg gcaccgcgtt
    25861 gtgctgttcg gcaaactggc agaagtggcg agcgaatatc tgcgtaaagg ttctcaggtt
    25921 tatatcgaag gtcagctgcg tacccgtaaa tggaccgatc aatccggtca ggatcgctac
    25981 accacagaag tcgtggtgaa cgttggcggc accatgcaga tgctgggtgg tcgtcagggt
    26041 ggtggcgctc cggcaggtgg caatatcggt ggtggtcagc cgcagggcgg ttggggtcag
    26101 cctcagcagc cgcagggtgg caatcagttc agcggcggcg cgcagtctcg cccgcagcag
    26161 tccgctccgg cagcgccgtc taacgagccg ccgatggact ttgatgatga cattccgttc
    26221 tgatttgtca ttaaaacaat aggttatatt gttttaaggt ggatgattaa agcatctgcc
    26281 agccataaaa aagaagcctc cgttatggag g
//
LOCUS       DADLSE010000045        26160 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLSE010000045 DADLSE010000000
VERSION     DADLSE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.045"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            223..417
                     /gene="bfd"
                     /locus_tag="JN692_001865"
     CDS             223..417
                     /gene="bfd"
                     /locus_tag="JN692_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7264189.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            490..966
                     /gene="bfr"
                     /locus_tag="JN692_001866"
     CDS             490..966
                     /gene="bfr"
                     /locus_tag="JN692_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7264190.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(963..1430)
                     /locus_tag="JN692_001867"
     CDS             complement(963..1430)
                     /locus_tag="JN692_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7264191.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1809..2120
                     /gene="rpsJ"
                     /locus_tag="JN692_001868"
     CDS             1809..2120
                     /gene="rpsJ"
                     /locus_tag="JN692_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7264192.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2153..2782
                     /gene="rplC"
                     /locus_tag="JN692_001869"
     CDS             2153..2782
                     /gene="rplC"
                     /locus_tag="JN692_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7264193.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2793..3398
                     /gene="rplD"
                     /locus_tag="JN692_001870"
     CDS             2793..3398
                     /gene="rplD"
                     /locus_tag="JN692_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7264194.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3395..3697
                     /gene="rplW"
                     /locus_tag="JN692_001871"
     CDS             3395..3697
                     /gene="rplW"
                     /locus_tag="JN692_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7264195.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3715..4536
                     /gene="rplB"
                     /locus_tag="JN692_001872"
     CDS             3715..4536
                     /gene="rplB"
                     /locus_tag="JN692_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7264196.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKLGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4553..4831
                     /gene="rpsS"
                     /locus_tag="JN692_001873"
     CDS             4553..4831
                     /gene="rpsS"
                     /locus_tag="JN692_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7264197.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4846..5178
                     /gene="rplV"
                     /locus_tag="JN692_001874"
     CDS             4846..5178
                     /gene="rplV"
                     /locus_tag="JN692_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7264198.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5196..5897
                     /gene="rpsC"
                     /locus_tag="JN692_001875"
     CDS             5196..5897
                     /gene="rpsC"
                     /locus_tag="JN692_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7264199.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5910..6320
                     /gene="rplP"
                     /locus_tag="JN692_001876"
     CDS             5910..6320
                     /gene="rplP"
                     /locus_tag="JN692_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7264200.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6320..6511
                     /gene="rpmC"
                     /locus_tag="JN692_001877"
     CDS             6320..6511
                     /gene="rpmC"
                     /locus_tag="JN692_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7264201.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6511..6765
                     /gene="rpsQ"
                     /locus_tag="JN692_001878"
     CDS             6511..6765
                     /gene="rpsQ"
                     /locus_tag="JN692_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7264202.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6930..7301
                     /gene="rplN"
                     /locus_tag="JN692_001879"
     CDS             6930..7301
                     /gene="rplN"
                     /locus_tag="JN692_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7264203.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7312..7626
                     /gene="rplX"
                     /locus_tag="JN692_001880"
     CDS             7312..7626
                     /gene="rplX"
                     /locus_tag="JN692_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7264204.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7641..8180
                     /gene="rplE"
                     /locus_tag="JN692_001881"
     CDS             7641..8180
                     /gene="rplE"
                     /locus_tag="JN692_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7264205.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8195..8500
                     /gene="rpsN"
                     /locus_tag="JN692_001882"
     CDS             8195..8500
                     /gene="rpsN"
                     /locus_tag="JN692_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7264206.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8534..8926
                     /gene="rpsH"
                     /locus_tag="JN692_001883"
     CDS             8534..8926
                     /gene="rpsH"
                     /locus_tag="JN692_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7264207.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8939..9472
                     /gene="rplF"
                     /locus_tag="JN692_001884"
     CDS             8939..9472
                     /gene="rplF"
                     /locus_tag="JN692_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7264208.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9482..9835
                     /gene="rplR"
                     /locus_tag="JN692_001885"
     CDS             9482..9835
                     /gene="rplR"
                     /locus_tag="JN692_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7264209.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9850..10353
                     /gene="rpsE"
                     /locus_tag="JN692_001886"
     CDS             9850..10353
                     /gene="rpsE"
                     /locus_tag="JN692_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7264210.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10357..10536
                     /gene="rpmD"
                     /locus_tag="JN692_001887"
     CDS             10357..10536
                     /gene="rpmD"
                     /locus_tag="JN692_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7264211.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10540..10974
                     /gene="rplO"
                     /locus_tag="JN692_001888"
     CDS             10540..10974
                     /gene="rplO"
                     /locus_tag="JN692_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7264212.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10982..12313
                     /gene="secY"
                     /locus_tag="JN692_001889"
     CDS             10982..12313
                     /gene="secY"
                     /locus_tag="JN692_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7264213.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12345..12461
                     /gene="rpmJ"
                     /locus_tag="JN692_001890"
     CDS             12345..12461
                     /gene="rpmJ"
                     /locus_tag="JN692_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7264214.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12608..12964
                     /gene="rpsM"
                     /locus_tag="JN692_001891"
     CDS             12608..12964
                     /gene="rpsM"
                     /locus_tag="JN692_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7264215.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12981..13370
                     /gene="rpsK"
                     /locus_tag="JN692_001892"
     CDS             12981..13370
                     /gene="rpsK"
                     /locus_tag="JN692_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7264216.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13404..14024
                     /gene="rpsD"
                     /locus_tag="JN692_001893"
     CDS             13404..14024
                     /gene="rpsD"
                     /locus_tag="JN692_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7264217.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14050..15039
                     /gene="rpoA"
                     /locus_tag="JN692_001894"
     CDS             14050..15039
                     /gene="rpoA"
                     /locus_tag="JN692_001894"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7264218.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15080..15463
                     /gene="rplQ"
                     /locus_tag="JN692_001895"
     CDS             15080..15463
                     /gene="rplQ"
                     /locus_tag="JN692_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7264219.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15570..15938
                     /locus_tag="JN692_001896"
     CDS             15570..15938
                     /locus_tag="JN692_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HAY7264220.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15949..16374
                     /gene="zntR"
                     /locus_tag="JN692_001897"
     CDS             15949..16374
                     /gene="zntR"
                     /locus_tag="JN692_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HAY7264221.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16430..16648
                     /locus_tag="JN692_001898"
     CDS             16430..16648
                     /locus_tag="JN692_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HAY7264222.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16645..17055)
                     /gene="mscL"
                     /locus_tag="JN692_001899"
     CDS             complement(16645..17055)
                     /gene="mscL"
                     /locus_tag="JN692_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAY7264223.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGGIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17185..18561)
                     /gene="trkA"
                     /locus_tag="JN692_001900"
     CDS             complement(17185..18561)
                     /gene="trkA"
                     /locus_tag="JN692_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7264224.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18583..19872)
                     /gene="rsmB"
                     /locus_tag="JN692_001901"
     CDS             complement(18583..19872)
                     /gene="rsmB"
                     /locus_tag="JN692_001901"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7264225.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(19918..20865)
                     /gene="fmt"
                     /locus_tag="JN692_001902"
     CDS             complement(19918..20865)
                     /gene="fmt"
                     /locus_tag="JN692_001902"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7264226.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20880..21389)
                     /gene="def"
                     /locus_tag="JN692_001903"
     CDS             complement(20880..21389)
                     /gene="def"
                     /locus_tag="JN692_001903"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7264227.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21520..22644
                     /gene="dprA"
                     /locus_tag="JN692_001904"
     CDS             21520..22644
                     /gene="dprA"
                     /locus_tag="JN692_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7264228.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGLLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22616..23089
                     /locus_tag="JN692_001905"
     CDS             22616..23089
                     /locus_tag="JN692_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7264229.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23118..23660
                     /locus_tag="JN692_001906"
     CDS             23118..23660
                     /locus_tag="JN692_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7264230.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23665..24237
                     /gene="tsaC"
                     /locus_tag="JN692_001907"
     CDS             23665..24237
                     /gene="tsaC"
                     /locus_tag="JN692_001907"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7264231.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24242..25060
                     /gene="aroE"
                     /locus_tag="JN692_001908"
     CDS             24242..25060
                     /gene="aroE"
                     /locus_tag="JN692_001908"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7264232.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEEVAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25057..25314
                     /locus_tag="JN692_001909"
     CDS             25057..25314
                     /locus_tag="JN692_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7264233.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25290..25844)
                     /locus_tag="JN692_001910"
     CDS             complement(25290..25844)
                     /locus_tag="JN692_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7264234.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6721 a         6196 c         6786 g         6457 t
ORIGIN      
        1 taccgttggc gcgggcgttg ttgctaaagt tctgggctaa ttacacgtta attagttttg
       61 aattgaaaag ggcgcttcgg cgcccttttt gctgcccgta atttgagctc gaaatattta
      121 gtcgtaattt tgctcgctga actatggcgc aaataaaact gtgctattgt aatcataacc
      181 attctcattt acactttgcg cagaatttga acgggagcaa tcatgtacgt ttgtttgtgt
      241 aatggtgtaa gtgacaaaaa aattcgccag gcagtccgtc agtttcatcc gcaatctttc
      301 caacaattgc gtaagtttat tcctgtggga aatcaatgcg gtaagtgcat tcgcgccgcg
      361 cgcgaagtga tgcaggacga gttgatgcag atgccagaat ttaaagagat tgcctgaagc
      421 ccacgcttat tttttgacat cccgctagcc ccatctacgc tctaatagtg gaagcggagg
      481 gactataaaa tgaaaggtga tgttaaaatc ataaattatc tcaataaact attgggaaat
      541 gagcttgtcg caatcaatca gtattttctc catgcccgga tgtttaaaaa ctggggcctg
      601 atgcgtctca atgacgtcga ataccatgaa tccatcgatg agatgaaaca cgctgataaa
      661 tatatcgagc gtattttgtt tttagaagga atccccaact tacaggattt aggcaaactg
      721 gggattggtg aagatgtcga ggagatgctg caatctgacc tgcgattaga actggaaggc
      781 gcgaaggatc tgcgtgaggc tatcgcatat gccgacagcg ttcatgacta tgttagccgc
      841 gatatgatga tcgagatcct tgctgatgaa gaaggtcata tcgactggct cgaaactgaa
      901 ctggatctca tcggtaaaat cggcctacaa aattacctgc aatcacaaat taaagttaaa
      961 gattaacggc ctatcagcaa actatcccag cctataacga aacccgcagc cgccagaaat
     1021 ggcccaaaag gcagcgggtt ttttaatgat tcttttcctc tcatgagcag accaataact
     1081 acggctccgc aagcgaatga ggcagcaagg aaaaccagtc gtggtaagaa ggcccatgaa
     1141 tgccaggcgc caagggcggc gagaaacttc acatcaccat agcctaatcc ttctttatgt
     1201 cgcagtatgc gataccccca gtaaatgaca gcgaatgtgc cgtaaccaac aattgccccc
     1261 cataacgcat cggctaaacc atcaggatga caaacttgat agaacaaaag acctgaccag
     1321 agtaacgggc acgtaaatct gtcaggtaac aagccatgct ttgcatccca gaaaaaaagt
     1381 agggctgata aacaagcgta caaaattaga aagggaaggg tagtgtccat aaaacatcct
     1441 ctgtaattgg agtatcacca tcgaataact ccggatgctc agcaacagag agagcgctga
     1501 tttgttaacc tgattccggt gaaaaacgct atcttcacaa ccttacaaat ttcgtggaat
     1561 gcagctcgca atctgcgcgt tgcgattaat ttctacccga tgcttgcgtc aggctcagat
     1621 ttgcgtataa tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa cctcagttag
     1681 ccgaatttgg ctacctaaca atgctcccaa tcggggagct acgtaagaac ggttacactc
     1741 tcccatcaat cgtaatgggt ctgaggagta atcattttcg tttataaaat aattggagct
     1801 ctggtctcat gcagaaccaa agaatccgta tccgcctgaa agcgtttgat catcgtctga
     1861 tcgatcaagc aaccgcggaa atcgtcgaga ctgccaagcg cactggtgcg caggtccgtg
     1921 gtccgatccc gctgccgaca cgcaaagagc gcttcactgt tctgatctcc ccgcacgtca
     1981 acaaagacgc gcgcgatcag tacgaaatcc gtactcactt gcgtctggtt gacatcgttg
     2041 agccaaccga gaaaaccgtt gatgctctga tgcgtctgga tctggctgcc ggtgtagacg
     2101 tgcagatcag cctgggttaa tcaggtcatt gagcgattga gaggttgaaa caatgattgg
     2161 tttagtcggt aaaaaagtgg gtatgacccg tatcttcaca gaagacggcg tttctatccc
     2221 agtaaccgta atcgaagttg aagcaaaccg cgttactcag gttaaagacc tggctaacga
     2281 tggctaccgt gctattcagg tgaccaccgg tgctaaaaaa gctaaccgtg tgaccaagcc
     2341 tgaagctggc cacttcgcta aagctggcgt agaagctggc cgtggtctgt gggaattccg
     2401 cctggctgaa ggcgaagagt tcactgtagg tcagagcatt agcgttgaac tgtttgctga
     2461 cgttaaaaaa gttgacgtaa ctggcacctc taaaggtaaa ggtttcgcag gtaccgttaa
     2521 gcgctggaac ttccgtaccc aggacgctac tcacggtaac tccttgtctc accgcgttcc
     2581 gggttctatc ggtcagaacc agactccggg caaagtgttc aaaggcaaga aaatggcagg
     2641 tcagatgggt aacgaacgtg taaccgttca gagccttgac gtagtacgcg ttgacgctga
     2701 gcgcaacctg ctgctggtta aaggtgctgt cccgggtgca accggtagcg acctgatcgt
     2761 taaaccagct gtgaaggcgt aaggagatag caatggaatt agtattgaaa gacgcgcaga
     2821 gcgcgctgac tgtttccgaa actaccttcg gtcgtgattt caacgaagcg ctggttcacc
     2881 aggttgttgt tgcttatgca gctggtgctc gtcagggtac tcgtgctcag aagactcgtg
     2941 ctgaagtaac tggttccggt aaaaaaccgt ggcgccagaa aggcaccggc cgtgcgcgtt
     3001 ctggttctat caagagcccg atctggcgtt ctggtggcgt gacctttgct gctcgtccgc
     3061 aggaccacag tcaaaaagtt aacaagaaga tgtaccgcgg cgcgctgaaa agcatcctgt
     3121 ccgaactggt acgtcaggat cgtctgatcg ttgtcgagaa gttctctgta gaagcgccga
     3181 aaactaagct gctggcacag aaactgaaag acatggctct ggaagatgtg ctgatcatca
     3241 ccggtgagct ggacgaaaac ctgttcctgg ctgcgcgcaa cctgcacaag gttgacgtac
     3301 gcgatgcaac tggtatcgac ccggttagcc tgatcgcctt cgacaaagtc gtaatgactg
     3361 ctgatgctgt taagcaagtt gaggagatgc tggcatgatt cgtgaagaac gtctgctgaa
     3421 ggtgctgcgt gcaccgcacg tttctgaaaa agcgtctact gcgatggaaa aatccaacac
     3481 catcgtactc aaagttgcca aagacgcgac caaagcagaa atcaaagctg ctgtgcagaa
     3541 actgtttgaa gtcgaagtcg aagtcgttaa caccctggta gttaaaggga aagttaaacg
     3601 tcacggacag cgtatcggtc gtcgtagcga ctggaaaaaa gcttacgtca ccctgaaaga
     3661 aggccagaat ctggacttcg ttggcggcgc tgagtaagtc ggaggagtaa tacaatggca
     3721 gttgttaaat gtaaaccgac atctccgggt cgtcgccacg tagttaaagt ggttaaccct
     3781 gagctgcaca agggcaaacc ttttgctccg ttgctggaaa aaaacagcaa atccggtggt
     3841 cgtaacaaca atggccgtat caccactcgt catatcggtg gtggccacaa gcaggcttac
     3901 cgtattgttg acttcaaacg caacaaagac ggtatcccgg cagttgttga acgtcttgag
     3961 tacgatccga accgttccgc gaacatcgcg ctggttctgt acaaagacgg tgaacgccgt
     4021 tacatcctgg cccctaaagg cctgaaagct ggcgaccaga ttcagtctgg cgttgatgct
     4081 gcaatcaaac taggtaacac cctgccgatg cgcaacatcc cggttggttc tactgttcat
     4141 aacgtagaaa tgaaaccagg taaaggcggt cagctggcac gttccgctgg tacttacgtt
     4201 cagatcgttg ctcgtgatgg tgcttatgtc accctgcgtc tgcgttctgg tgaaatgcgt
     4261 aaagtagaag cagactgccg tgcaactctg ggcgaagttg gcaatgctga gcatatgctg
     4321 cgcgttctgg gtaaagcagg tgctgcacgc tggcgtggtg ttcgtccgac cgttcgcggt
     4381 accgcgatga acccggtaga ccacccacat ggtggtggtg aaggtcgtaa ctttggtaag
     4441 cacccggtaa ctccgtgggg cgttcagacc aaaggtaaga agacccgcag caacaagcgt
     4501 actgataaat tcatcgtacg tcgccgtagc aaataatttt agaggataag ccatgccacg
     4561 ttctctcaag aaaggtcctt ttattgacct gcacttgctg aagaaggtag agaaagcggt
     4621 ggaaagcgga gacaagaagc ccctgcgcac ttggtcccgt cgttcaacga tctttcctaa
     4681 catgatcggt ttgaccatcg ctgtccataa tggtcgtcag cacgttccgg tatttgtaac
     4741 cgacgaaatg gtcggtcaca aactgggtga attcgcaccg actcgtactt atcgcggcca
     4801 cgctgctgat aaaaaagcga agaagaaata aggtaggagg aagagatgga aactatcgct
     4861 aaacatcgcc atgctcgttc ttctgctcag aaggttcgcc ttgttgctga cctgattcgc
     4921 ggtaagaaag tgtcgcaggc tctggatatt ttgacctaca ccaacaagaa agcggctgta
     4981 ctggtcaaga aagttctgga atctgccatt gctaacgctg aacacaacga tggcgctgac
     5041 attgacgatc tgaaagttac gaaaattttc gtagacgaag gcccgagcat gaagcgcatt
     5101 atgccgcgtg caaaaggtcg tgcagatcgc atcctgaagc gcaccagcca catcactgtg
     5161 gttgtgtccg atcgctgaga ctctggagac tagcaatggg tcagaaagta catcctaatg
     5221 gtattcgctt gggtattgta aaaccatgga actctacctg gtttgcgaac accaaagaat
     5281 tcgctgacaa cctggacagc gattttaaag tacgtcagta cctgactaag gaactggcta
     5341 aagcgtccgt atctcgtatc gttatcgagc gtccggctaa gagcatccgt gtaaccattc
     5401 acactgctcg cccgggtatc gttatcggta aaaaaggtga agacgtagaa aaactgcgta
     5461 aggtcgtagc ggacatcgct ggcgttcctg cacagatcaa catcgccgaa gttcgtaagc
     5521 ctgaactgga cgcaaaactg gttgctgaca gcatcacttc tcagctggaa cgtcgcgtta
     5581 tgttccgtcg tgctatgaag cgtgctgtac agaacgcaat gcgtctgggc gctaaaggta
     5641 ttaaagttga agttagcggc cgtctgggcg gcgcggaaat cgcacgtacc gaatggtacc
     5701 gcgaaggtcg cgtaccgctg cacactctgc gtgctgacat cgactacaac acctctgaag
     5761 cgcacaccac ttacggtgta atcggcgtta aagtgtggat cttcaaaggc gagatcctgg
     5821 gtggtatggc tgctgttgaa caaccggaaa aaccggctgc tcagcctaaa aagcagcagc
     5881 gtaaaggccg taaataagga gcgtcgctga tgttacaacc aaagcgtaca aaattccgta
     5941 aaatgcacaa aggccgtaac cgcggtctgg cgcagggtac ggatgttagc ttcggcagct
     6001 tcggtctgaa agctgttggc cgtggtcgtc tgactgcccg tcagatcgaa gcagcacgtc
     6061 gtgctatgac ccgtgcagtt aagcgtcaag gtaagatctg gatccgtgtg ttcccggaca
     6121 aaccgatcac tgaaaagccg ctggcagtgc gtatgggtaa aggtaaaggt aacgtggagt
     6181 attgggttgc cttgattcag ccgggtaaag tcctgtatga aatggacggt gttccggaag
     6241 agctggcccg tgaagcattc aagctggcag cagcgaaact gccgattaaa accacctttg
     6301 taactaagac ggtgatgtaa tgaaagcaaa agagctgcgt gagaagagca ttgaagagct
     6361 gaacaccgag ctgctgaacc tgctgcgtga gcagttcaac ctgcgtatgc aggctgcaag
     6421 tggccagctg caacagtctc acctgttgaa gcaagtgcgt cgcgatgtcg cacgcgttaa
     6481 gactttactg aacgagaagg cgggtgcgta atgaccgata aaatccgtac tctgcaaggt
     6541 cgcgttgtta gcgacaaaat ggagaaatcc attgttgttg ctatcgaacg ttttgtgaaa
     6601 cacccgatct acggtaaatt catcaagcgt acgaccaaac tgcacgtaca tgacgagaac
     6661 aacgaatgcg gtatcggtga cgtggttgaa atccgcgaat gccgtccgct gtccaagact
     6721 aaatcctgga cgctggttcg cgttgtagag aaagctgttc tgtaatacag tacactctct
     6781 cgatacgaat aaacggctca gaaatgagcc gtttattttt tctacccata tccttgaagc
     6841 ggtgttataa tgccgcgccc tcgatatggg gatttttaac gacctgattt tcgggtctca
     6901 gtagtagttg acattagcgg agcactaaaa tgatccaaga acagactatg ctgaacgtcg
     6961 ccgacaactc cggtgcacgt cgcgtaatgt gtatcaaggt tctgggtggc tcgcaccgtc
     7021 gctacgcagg cgtaggcgac atcatcaaga tcaccatcaa agaagcaatt ccgcgtggta
     7081 aggtcaaaaa aggtgatgtg ctgaaggcgg tagtggtgcg caccaagaag ggtgttcgtc
     7141 gcccggacgg ttctgtcatt cgcttcgatg gtaatgcttg tgttcttctg aacaacaaca
     7201 gcgagcagcc tatcggtacg cgtatttttg ggccggtaac tcgtgagctt cgtagtgaga
     7261 agttcatgaa aattatctct ctggcaccag aagtactcta aggagcgaat catggcagcg
     7321 aaaatccgtc gtgatgacga agttatcgtg ttaaccggta aagataaagg taaacgcggt
     7381 aaagttaaga atgtcctgtc ttccggcaag gtcattgttg aaggtatcaa cctggttaag
     7441 aaacatcaga agccggttcc ggccctgaac caaccgggtg gcatcgttga aaaagaagcc
     7501 gctattcagg tttccaacgt agcaatcttc aatgcggcaa ccggcaaggc tgaccgtgta
     7561 ggctttagat tcgaagacgg caaaaaagtc cgtttcttca agtctaacag cgaaactatc
     7621 aagtaatttg gagtagtacg atggcgaaac tgcatgatta ctacaaagac gaagtagtta
     7681 aaaaactcat gactgagttt aactacaatt ctgtcatgca agtccctcgg gtcgagaaga
     7741 tcaccctgaa catgggtgtt ggtgaagcga tcgctgacaa aaaactgctg gataacgcag
     7801 cagcagacct ggcagcaatc tccggtcaaa aaccgctgat caccaaagca cgcaaatctg
     7861 ttgcaggctt caaaatccgt cagggctatc cgatcggctg taaagtaact ctgcgtggcg
     7921 aacgcatgtg ggagttcttt gagcgcctga tcactattgc tgtacctcgt atccgtgact
     7981 tccgtggcct gtccgctaag tctttcgacg gtcgtggtaa ctacagcatg ggtgtccgtg
     8041 agcagatcat cttcccagaa atcgactacg ataaagtcga ccgcgttcgt ggtttggata
     8101 ttaccattac cactactgcg aaatctgacg aagaaggccg cgctctgctg gctgcctttg
     8161 acttcccgtt ccgcaagtaa ggtagggtta ctaaatggct aagcaatcaa tgaaagcacg
     8221 cgaagtaaaa cgcgtagctt tagctgataa atacttcgcg aaacgcgctg aactgaaagc
     8281 gatcatctct gatgtgaacg cttccgacga agatcgttgg aacgctgttc tcaagctgca
     8341 gactctgccg cgtgattcca gcccgtctcg tcagcgtaac cgctgccgtc aaacaggtcg
     8401 tccgcatggt ttcctgcgga agttcgggtt gagccgtatt aaggtccgtg aagccgctat
     8461 gcgcggtgaa atcccgggtc tgaaaaaggc tagctggtaa ttgtcaccaa ttgaatcacg
     8521 ggagtaaaga cagatgagca tgcaagatcc gatcgcggat atgctgaccc gtatccgtaa
     8581 cggtcaggcc gcgaacaaag ctgcggtcac catgccttcc tccaagctga aagtggcaat
     8641 cgccaacgtg ctgaaggaag aaggttttat tgaagatttt aaagttgaag gcgacaccaa
     8701 gcctgaactg gaacttactc tgaagtattt ccagggcaaa gctgttgtag aaagcattca
     8761 gcgtgtcagc cgcccaggtc tgcgcatcta taaacgtaaa gatgagctgc cgaaagttat
     8821 ggcgggtctg ggtatcgcag ttgtttctac ctctaaaggt gttatgactg atcgtgcagc
     8881 gcgccaggct ggtcttggtg gcgaaattat ctgctacgta gcctaatcgg aggaaaaaat
     8941 gtctcgtgtt gctaaagcac cggtcgttgt tcctgccggc gttgacgtaa aaatcaacgg
     9001 tcaggttatt acgatcaaag gtaaaaacgg cgagctgact cgtactctca acgatgctgt
     9061 tgaagttaaa catgcagata ataccctgac cttcggtccg cgtgatggtt acgcagacgg
     9121 ttgggcacag gctggtaccg cgcgtgccct gctgaactca atggttatcg gtgttaccga
     9181 aggcttcact aagaagctgc agctggttgg tgtaggttac cgtgcagcgg ttaaaggcaa
     9241 tgtgattaac ctgtctctgg gtttctctca tcctgttgac catcagctgc ctgcgggtat
     9301 cactgctgaa tgtccgactc agactgaaat cgtgctgaaa ggcgctgata agcaggtgat
     9361 cggccaggtt gcagcggatc tgcgcgccta ccgtcgtcct gagccttaca aaggcaaggg
     9421 tgttcgttac gccgacgaag tcgtgcgtac caaagaggct aagaagaagt aaggtaacac
     9481 tatggataag aaatctgctc gtatccgtcg tgcgacccgc gcacgccgca agctccagga
     9541 gctgggcgca actcgcctgg tggtacatcg taccccgcgt cacatttacg cacaggtaat
     9601 tgcaccgaac ggttctgaag ttctggtagc tgcttctact gtagaaaaag ctatcgctga
     9661 acaactgaag tacaccggta acaaagacgc ggctgcagct gtgggtaaag ctgtcgctga
     9721 acgcgctctg gaaaaaggca tcaaagatgt atcctttgac cgttccgggt tccaatatca
     9781 tggtcgtgtc caggcactgg cagatgctgc ccgtgaagct ggccttcagt tctaaggtag
     9841 aggtgtaaga tggctcacat cgaaaaacaa gctggcgaac tgcaggaaaa gctgatcgcg
     9901 gtaaaccgcg tatctaaaac cgttaaaggt ggtcgtattt tctccttcac agctctgact
     9961 gtagttggcg atggtaacgg tcgcgttggt tttggttacg gtaaagcgcg tgaagttcca
    10021 gcagcgatcc agaaagcgat ggaaaaagcc cgtcgcaata tgattaacgt cgcgctgaat
    10081 aacggcactc tacaacaccc tgttaaaggt gttcacacgg gttctcgcgt attcatgcag
    10141 ccggcttccg aaggtaccgg tatcatcgcc ggtggtgcaa tgcgcgccgt tctggaagtc
    10201 gctggggttc ataacgttct ggctaaagca tatggttcca ccaacccgat caacgtggtt
    10261 cgtgcaacta ttgatggcct ggaaaatatg aattctccag aaatggtcgc tgccaagcgt
    10321 ggtaaatccg ttgaagaaat tctggggaaa taaaccatgg caaagactat taaaattact
    10381 caaacccgca gtgcaatcgg tcgtctgccg aaacacaagg caacgctgct tggcctgggt
    10441 ctgcgtcgta ttggtcacac cgtagagcgc gaggatactc ctgctattcg cggtatgatc
    10501 aacgcggttt ccttcatggt taaagttgag gagtaagaga tgcgtttaaa tactctgtct
    10561 ccggccgaag gctccaaaaa ggcgggtaaa cgcctgggtc gtggtatcgg ttctggcctc
    10621 ggtaaaaccg gtggtcgtgg tcacaaaggt cagaagtctc gttctggcgg tggcgtacgt
    10681 cgcggtttcg agggtggtca gatgcctctg taccgtcgtc tgccgaaatt cggcttcact
    10741 tctcgtaaag cagcgattac agccgaagtt cgtctgtctg acctggctaa agtagaaggc
    10801 ggtgttgtag acctgaacac gctgaaagcg gctaacatta tcggtatcca gatcgagttc
    10861 gcgaaagtga tcctggctgg cgaagtaacg actccggtaa ctgttcgtgg cctgcgtgtt
    10921 actaaaggcg ctcgtgctgc tatcgaagct gctggcggta aaatcgagga ataagtagca
    10981 gatggctaaa caaccgggat tagattttca aagtgccaaa ggtggcttag gcgagctgaa
    11041 acgcagactg ctgtttgtta tcggtgcgct gattgtgttc cgtattggct cttttattcc
    11101 gatccctggt attgatgccg ctgtacttgc caaactgctt gagcaacagc gaggcaccat
    11161 cattgagatg tttaacatgt tctctggtgg tgctctcagc cgtgcttcta tctttgctct
    11221 ggggatcatg ccgtatattt cggcgtcgat cattatccag ctgctgacgg tggttcaccc
    11281 aacgttggca gaaattaaga aagaagggga gtctggtcgt cgtaagatca gccagtacac
    11341 ccgttacggt actctggtgc tggcaatatt ccagtcgatc ggtattgcta ccggtctgcc
    11401 gaatatgcct ggtatgcaag gcctggtgat taacccgggc tttgcattct acttcaccgc
    11461 tgttgtaagt ctggtcacag gaacgatgtt cctgatgtgg ttgggcgaac agatcactga
    11521 acgaggtatc ggcaacggta tttcaatcat tatcttcgcc ggtattgtcg cgggactccc
    11581 gccagccatt gcccatacta tcgagcaagc gcgtcaaggc gacctgcact tcctcgtgtt
    11641 gctgttggtt gcagtattag tatttgcagt gacgttcttt gttgtgtttg ttgagcgtgg
    11701 tcaacgccgc attgtggtaa actacgcgaa acgtcagcaa ggtcgtcgtg tctatgctgc
    11761 acagagcaca catttaccgc tgaaagtgaa tatggcgggg gtaatcccgg caatcttcgc
    11821 ttccagtatt attctgttcc cggcgaccat cgcgtcatgg ttcgggggcg gtactggttg
    11881 gaactggctg acaacaattt cgctgtattt gcagcctggg caaccgcttt atgtgttact
    11941 ctatgcgtct gcaatcatct tcttctgttt cttctacacg gcgttggttt tcaacccgcg
    12001 tgaaacagca gataacctga agaagtccgg tgcatttgta ccaggaattc gtccgggaga
    12061 gcaaacggcg aagtatatcg ataaagtaat gacccgcctg accctggttg gtgcgctgta
    12121 tattaccttt atctgcctga tcccggagtt catgcgtgat gcaatgaaag taccgttcta
    12181 cttcggtggg acctcactgc ttatcgttgt tgtcgtgatt atggacttta tggctcaagt
    12241 gcaaactctg atgatgtcca gtcagtatga gtctgcattg aagaaggcga acctgaaagg
    12301 ctacggccga taattggtcg cccgagaagt tacggagagt aaaaatgaaa gttcgtgctt
    12361 ccgtcaagaa attatgccgt aactgcaaaa tcgttaagcg tgatggtgtc atccgtgtga
    12421 tttgcagtgc cgagccgaag cataaacagc gccaaggctg attttttcgc atatttttct
    12481 tgcaaagttg ggttgagctg gctagattag ccagccaatc ttttgtatgt ctgtgcgttt
    12541 ccatttgagt atcctgaaaa cgggcttttc agcatggaac gtacatatta aatagtagga
    12601 gtgcatagtg gcccgtatag caggcattaa cattcctgat cataagcatg ccgtaatcgc
    12661 attaacttcg atttatggcg tcggcaagac ccgttctaaa gccatcctgg ctgcagcggg
    12721 tatcgctgaa gatgttaaga tcagtgagct gtctgaagga caaatcgaca cgctgcgtga
    12781 cgaagttgcc aaatttgtcg ttgaaggtga tctgcgccgt gaaatcagca tgagcatcaa
    12841 gcgcctgatg gatcttggtt gctatcgcgg tttgcgtcat cgtcgtggtc tcccggttcg
    12901 cggtcagcgt accaagacca acgcacgtac ccgtaagggt ccgcgcaaac cgatcaagaa
    12961 ataatcgggg tgattgaata atggcaaagg caccaattcg tgcacgtaaa cgtgtaagaa
    13021 aacaagtctc tgacggcgtg gctcatatcc atgcttcttt caacaacacc atcgtgacta
    13081 tcactgatcg tcagggtaac gcgttgggtt gggcaacagc cggtggttcc ggtttccgtg
    13141 gttctcgcaa atccactccg tttgcagctc aggttgcagc agagcgttgc gctgacgccg
    13201 tgaaagaata cggcatcaag aatctggaag ttatggttaa aggtccgggt ccaggccgcg
    13261 aatctactat tcgtgctctg aacgccgcag gtttccgcat cactaacatt actgatgtga
    13321 ctccgatccc tcataacggt tgtcgtccgc cgaaaaaacg tcgcgtataa cgcctcgttt
    13381 tccaggtttg ttggagaaag aaaatggcaa gatatttggg tcctaagctc aagctgagcc
    13441 gtcgtgaggg caccgactta ttccttaagt ctggcgttcg cgcgatcgat accaagtgta
    13501 aaattgaaca agctcctggc cagcacggtg cgcgtaaacc gcgtctgtct gactatggtg
    13561 tgcagttgcg tgaaaagcaa aaagttcgcc gtatctatgg tgtgctggag cgtcagttcc
    13621 gtaactacta caaagaagca gcacgtctga aaggcaacac cggtgaaaac ctgttggctc
    13681 tgctggaagg tcgtctggac aacgttgtat accgtatggg cttcggtgcc actcgtgcag
    13741 aagcacgtca gctggttagc cataaagcaa ttatggtaaa cggtcgtgtt gttaacatcg
    13801 cttcttatca ggttagtccg aatgacgttg taagcattcg tgagaaagcg aagaagcagt
    13861 ctcgcgtgaa agccgctctg gagctggctg agcagcgtga aaagccaact tggctggaag
    13921 ttgatgctgg caagatggaa ggtacgttta agcgtaagcc ggagcgttct gatctgtctg
    13981 cggacattaa cgaacacctg atcgtcgagc tttactccaa gtaaagctta gtaccaaaga
    14041 gaggacacaa tgcagggttc tgtgacagag tttctaaaac cgcgcctggt tgatatcgag
    14101 caagtgagtt cgacgcacgc caaggtgacc cttgagcctt tagagcgtgg ctttggccat
    14161 actctgggta acgcactgcg ccgtattctg ctctcatcga tgccgggttg cgcggtgacc
    14221 gaggttgaga ttgatggtgt actacatgag tacagcacca aagaaggcgt tcaggaagat
    14281 atcctggaaa tcctgctcaa cctgaaaggg ctggcggtga gagttcaggg caaagatgaa
    14341 gttattctta ccttgaataa atctggcatt ggccctgtga ctgcagccga tatcacccac
    14401 gacggtgatg tcgaaatcgt caagccgcag cacgtgatct gccacctgac cgatgagaac
    14461 gcgtctatta gcatgcgtat caaagttcag cgcggtcgtg gttatgtgcc ggcttctacc
    14521 cgaattcatt cggaagaaga tgagcgccca atcggccgtc tgctggtcga cgcatgctac
    14581 agccctgtgg agcgtattgc ctacaatgtt gaagcagcgc gtgtagaaca gcgtaccgac
    14641 ctagacaagc tggtcatcga aatggaaacc aacggcacaa tcgatcctga agaggcgatt
    14701 cgtcgtgcgg caaccattct ggctgaacaa ctggaagctt tcgttgactt acgtgatgta
    14761 cgtcagcctg aagtgaaaga agagaaacca gagttcgatc cgatcctgct gcgccctgtt
    14821 gacgatctgg aattgactgt ccgctctgct aactgcctta aagcagaagc tatccactat
    14881 atcggtgatc tggtacagcg taccgaggtt gagctcctta aaacgcctaa ccttggtaaa
    14941 aaatctctta ctgagattaa agacgtgctg gcttcccgtg gactgtctct gggcatgcgc
    15001 ctggaaaact ggccaccggc aagcatcgct gacgagtaac cggatcacag gttaaggttt
    15061 tactgagaag gataaggtca tgcgccatcg taagagtggt cgtcaactga accgcaacag
    15121 cagccatcgc caggctatgt tccgcaatat ggcaggttca ctggttcgtc atgaaatcat
    15181 caagacgact ctgcctaaag cgaaagagct gcgccgcgta gttgagccgc tgattactct
    15241 tgccaagact gatagcgttg ctaatcgtcg tctggcattc gcccgtactc gtgataacga
    15301 gatcgtggca aaactgttta acgaactggg cccgcgtttc gcgagccgtg ccggtggtta
    15361 cactcgtatt ctgaagtgtg gcttccgtgc aggcgacaac gcgccgatgg cttacatcga
    15421 gctggttgat cgttcagaga aagcagaagc tgctgcagag taatctgaag caacgtaaaa
    15481 aaacccgccc cggcgggttt ttttataccc gtagtatccc cacttatcta caatagctgt
    15541 actctttttg ttcatcccct ggagtattta tgtggttact tgaccagtgg gcagagcgcc
    15601 atatagcaga agcgcaagcg aaaggtgagt ttgataacct ggcaggtagc ggcgaaccat
    15661 tgatactgga tgatgattct cacgtgccac cggaattacg tgcggggtat cgcttgctga
    15721 agaatgccgg ttgcttaccg ccagaacttg agcaacggag agaagcaatt cagcttctgg
    15781 atattctcaa aggtatccgt cacgatgatc cgcaatatca agaggttagc cgtcgattgt
    15841 cattactgga attgaagctg cgacaagctg gattgagtac cgatttttta cgcggcgatt
    15901 atgctgacaa gttgttggac aaaatcaacg ataactagtg gagtatgtat gtatcgcatt
    15961 ggtgagctgg caaaaatggc ggaagtaaca cctgacacga ttcgttatta cgaaaaacag
    16021 cagatgatgg agcatgaagt gcgtactgaa ggcgggtttc gcctgtatac cgaaagcgat
    16081 ctccagcgat tgaaatttat ccgccatgcc agacaactag gtttcagtct ggagtcgatc
    16141 cgcgagttgc tgtcgatccg catcgatcct gaacaccata cctgtcagga gtcaaaaggc
    16201 attgtgcagg aaagattgca ggaagtcgaa gcacggatag ccgagttgca gagtatgcag
    16261 cgttccttgc aacgccttaa cgatgcctgt tgtgggaccg cccatagcag tgtttattgc
    16321 tcgattcttg aagctcttga acaaggggcg agtggcgtta agagtggctg ttgatttttt
    16381 gcactgacag gattacactc gcgccgttaa ataaccaact ggagttttta tgagccgata
    16441 tcagcatact aaagggcaga taaaggataa tgcgatagaa gcattactac atgatccctt
    16501 attccgacag cgagtagaga aaaataagaa agggaaaggc agttacatgc gaaaaggaaa
    16561 acatggcaat cgggggaact gggaggccag tggcaagaaa gtaaatcact tttttaccac
    16621 tggtcttctg ctttcaggcg cttgttaaga gcggttattc tgctctttca gcaaatcacg
    16681 aatttctgcc agtaatactt cttctttagt tggtgcaggt gcggcagctg gttcttcttt
    16741 tttccgattc agtttgttga ttagcttaat cgccataaag atggcaaagg ccacaatcag
    16801 aaaatcaaag acgttttgaa tgaagacacc gtaatgcatc acaacagcag ggataccccc
    16861 ctgcgcatcg cgtagcgtga cagcaaactg tttaaagtcg atcccgccaa ttaataagcc
    16921 cagaggtggc atgatgatat cggcaaccag tgaagataca atcttcccga atgccgcacc
    16981 gataatgaca cccaccgcca aatccaccac gttcccgcgc attgcaaatt cgcgaaattc
    17041 tttaataatg ctcatgttat tctccctatg aagccgacaa ccataagtct aacaaatgtt
    17101 aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag ggaaataata
    17161 aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga agagtctttc
    17221 gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc cttgctcaat
    17281 gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc caataatcgt
    17341 tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc
    17401 accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac
    17461 aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat
    17521 tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct ggatcaatac
    17581 catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat tggcctcgtc
    17641 atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg ccagcagttc
    17701 ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg ccagttcggc
    17761 agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca gacgacgcgc
    17821 cagccctgca ccgatattac cgccaccaac cagcatgatc cgcttatacg gtttttccag
    17881 tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa agaacacttc
    17941 atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt ggcggaaaat
    18001 tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa gtgcattacc
    18061 aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac cctcagcgaa
    18121 gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat cgataaccaa
    18181 ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata gcttatcggc
    18241 atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt tgaaaagtga
    18301 gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag caaccagcat
    18361 atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat gcccctgcac
    18421 gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg tatcgacaac
    18481 agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa cctggccggc
    18541 acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt tgattagctt
    18601 agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac ccggttgctc
    18661 tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg
    18721 caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc caccggattt
    18781 taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt ccgggatatc
    18841 gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg ttgctgaaca
    18901 aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg aagggtaacg
    18961 gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt cgtaaacgcg
    19021 agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg ccacctcaag
    19081 gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt caccgttttg
    19141 tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc
    19201 ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag catcagggta
    19261 atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc agctgtcgcg
    19321 ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat tggcttcgac
    19381 gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg
    19441 cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc gacgctggaa
    19501 ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg cgatagcgcc
    19561 ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca gttgatacaa
    19621 accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac gggccattaa
    19681 cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc acaactcttg
    19741 aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa
    19801 tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac gtaaattacg
    19861 ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag tggactatca
    19921 gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt cttgcgcgct
    19981 catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat caccagtcgc
    20041 aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt
    20101 atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca gccagctcat
    20161 tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa gtgaccagtc
    20221 aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac
    20281 ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc cttgtgggcc
    20341 aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcagga
    20401 gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa tggttacacc
    20461 agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg
    20521 cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca cagctttagg
    20581 cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg cgaccagttg
    20641 ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt cttcctcagc
    20701 cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg
    20761 ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc
    20821 aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag ttcttatcct
    20881 taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg ttgttgtttc
    20941 agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc atgctgaata
    21001 cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg gtcaagggcg
    21061 cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat cgacaggcaa
    21121 ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc
    21181 cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat atcaacctgg
    21241 gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata
    21301 cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc
    21361 ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa
    21421 agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac tgaccaatcg
    21481 caaagattgc taaggcttgc ttatggcagg gagataagga tggtcgatac agaaatttgg
    21541 ctgcgtttaa tgagcatcag cagcttgtac ggcgatgata tggtccgtat cgctcactgg
    21601 ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaacagggct tacattgcgg
    21661 caggcacaac gctttctttc atttccacga aagagtatcg aaagctcact ttgttggttg
    21721 gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc tcaacttcag
    21781 gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc gctgcattca
    21841 tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg atgggggcga
    21901 ttattttgcg aaactctggc gacgtgtgga gtgacaatta cgagtggact ggcgcgtgga
    21961 atcgatggtg tggtgcataa agcggcctta caggtaaatg gcgtcagcat tgctgtattg
    22021 gggaatggac ttaataccat tcatccgcgt cgccatgccc gactggctgc cagtctgttt
    22081 gaacagggtg gcgctctcgt ctcggaattt cccctcgatg ttccacccct tgcttacaat
    22141 ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt ggtggaagcg
    22201 gctttgcgca gtggtttgct ggtgacagca cgttgtgcgc ttgagcaggg gcgtgaagtt
    22261 tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg gttaataaaa
    22321 caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca atttggattg
    22381 cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca agacgtggca
    22441 ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt tgacgtcgtc
    22501 gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga actggagtta
    22561 gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc atgccatgtt
    22621 cgacgtacta atgtatttgt ttgaaaccta tattcacact gaagctgagt tgcgtgtgga
    22681 tcaagacaaa cttgaacagg atcttaccga cgcaggtttt gagcgagaag atatctacaa
    22741 tgccctgcta tggctggaaa aacttgctga ttatcaggaa gggttggcag aaccgatgca
    22801 actggcctcg gatcctctct ccatgcgtat ttatacaccg gaagagtgtg aacgactgga
    22861 tgccagctgc cgtgggtttc tgcttttcct tgagcagatt caggtgctca accttgaaac
    22921 tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg aactggatga
    22981 tctgaaatgg gtgatcctga tggtgttgtt caacatcccg ggctgcgaaa atgcgtacca
    23041 gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat catttcttta
    23101 attcagcata agttgttatg gcaaaatcag cactgttcac ggtgcgtaat aatgagtcct
    23161 gcccaaagtg cggggctgaa ctggttattc gatccggaaa acacggtccg tttcttggat
    23221 gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg gatggacata
    23281 ttgtcaaagt tctggagggg caggtttgcc ccgtatgtgg cgcaaatctg gtattacgcc
    23341 agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa cataccgaac
    23401 ttatcgataa accggacgaa acagcaatta catgccccca atgtcggacg ggccatctgg
    23461 tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac ccggagtgtc
    23521 aatttgccat taacttcaaa ccaatagctg gagaatgccc tgagtgtcat tatccgctac
    23581 tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa caatgtggaa
    23641 agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc tatcgcagct
    23701 gcgatagatg tcctcaatga aaaacgtgtc atcgcctatc caacggaagc cgttttcggt
    23761 gtcgggtgcg atcctgatag cgaaacagca gtgatgagac tgttggagtt aaaacagcgt
    23821 ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa accctatatt
    23881 gatgacacca tgttgactga cgtgcagcgt gaaaccattt tttcctgctg gccaggtcct
    23941 gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg ccgctttgat
    24001 tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca ggcttatggt
    24061 aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg aacagtagac
    24121 gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac gggggggcgt
    24181 ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca ggggtaacat
    24241 aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc cattcattca
    24301 tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt tggcacccat
    24361 caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag gtgcgaatgt
    24421 gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg aacgggcagc
    24481 gttggctggt gcagttaata ccctcaagcg gttagaagat ggacgcctgc tgggtgacaa
    24541 taccgatggt gtaggcttgt taagcgatct ggaacgtctg tcttttatcc gccctggttt
    24601 acgtattctg cttatcggcg ctggtggagc atctcgcggc gtactactgc cacttctttc
    24661 cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag aggtggctaa
    24721 attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg aaggtcatga
    24781 gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc cggcgatccc
    24841 gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga aaggaaaaac
    24901 tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg gtttaggaat
    24961 gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc ctgacgtaga
    25021 accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat ccagtttccg
    25081 gacagggaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct cgtgaatggt
    25141 atgcaactga catgcgcgat ctctggcgag agtctggcgt atcgctttac tggagatacg
    25201 ccagaacagt ggttagcgag ttttcgtcag catcgctggg acctggaaga agaagcggaa
    25261 aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc ctgatccaga
    25321 tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc cttctcttca
    25381 tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc gctctccagc
    25441 cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc tactattgcg
    25501 ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt gcagccgtgg
    25561 agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt gccatctggg
    25621 ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt ggtgcgtgct
    25681 ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat ccccacatca
    25741 tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac gcgctgaccg
    25801 atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac ctcagcaata
    25861 aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt tagtatgcaa
    25921 aaaagcaccg ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca aacagaaaaa
    25981 aagatcaaaa aaatacttgt gcaaaatatt gggatcccta taatgcgcct ccgttgagac
    26041 gacaacgtga aacacttcac aggatggtcg gaacaacgaa gagaaaaaat cctgaaattc
    26101 agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt gagctgaaag
//
LOCUS       DADLSE010000046        25651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLSE010000046 DADLSE010000000
VERSION     DADLSE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.046"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..602)
                     /locus_tag="JN692_001911"
     CDS             complement(<1..602)
                     /locus_tag="JN692_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7264235.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALVVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1050..2021
                     /locus_tag="JN692_001912"
     CDS             1050..2021
                     /locus_tag="JN692_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7264236.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="JN692_001913"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="JN692_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7264237.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="JN692_001914"
     CDS             3494..4816
                     /locus_tag="JN692_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7264238.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="JN692_001915"
     CDS             complement(4998..7058)
                     /locus_tag="JN692_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7264239.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLTPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN692_001916"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="JN692_001916"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7264240.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQLLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7908..9056
                     /locus_tag="JN692_001917"
     CDS             7908..9056
                     /locus_tag="JN692_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7264241.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKGADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="JN692_001918"
     CDS             complement(9152..10648)
                     /locus_tag="JN692_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7264242.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADSLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /locus_tag="JN692_001919"
     CDS             complement(10869..12155)
                     /locus_tag="JN692_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7264243.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVEVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN692_001920"
     CDS             complement(12339..14327)
                     /gene="hmsP"
                     /locus_tag="JN692_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7264244.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVNAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN692_001921"
                     /pseudo
     CDS             complement(14409..17882)
                     /gene="bcsC"
                     /locus_tag="JN692_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN692_001922"
     CDS             complement(17864..18970)
                     /gene="bcsZ"
                     /locus_tag="JN692_001922"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7264245.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTGPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQDWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN692_001923"
     CDS             complement(18977..21316)
                     /gene="bcsB"
                     /locus_tag="JN692_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7264246.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNKFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLSTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN692_001924"
                     /pseudo
     CDS             complement(21327..23929)
                     /gene="bcsA"
                     /locus_tag="JN692_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN692_001925"
     CDS             complement(23926..24678)
                     /gene="bcsQ"
                     /locus_tag="JN692_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7264247.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKANGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(24690..24878)
                     /locus_tag="JN692_001926"
     CDS             complement(24690..24878)
                     /locus_tag="JN692_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264248.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25151..>25651
                     /locus_tag="JN692_001927"
     CDS             25151..>25651
                     /locus_tag="JN692_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7264249.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRS"
BASE COUNT         6015 a         7100 c         6460 g         6076 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccaccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgt cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagatcgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg agttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcgaatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc tgtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa cctctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtcttgtaag gttatggggg tacctgcggc cttaaaataa cacccagaca acatcacaga
    12241 aatgtacctg gatcataatt gaaacaaaaa ggttaaaaag tttgagctgg ctcgcacaaa
    12301 ccagcacttt ttaaagtttt gtaatcagtt tggggtagct acttttcttc caggtaactc
    12361 tcttcgaaga tttcaatagg gagtgggcga gcaaaaagga agccctgggc aataccaacg
    12421 cccgcttttg ccagccagtc tcgttgtgct tcagtctcca cgccttcggc aatcatttgt
    12481 aagttcaggc tctgcgccag catgataatt gcagcaatca tgctgctatc ttccggcaag
    12541 ccttcaacaa acattttgtc gattttcagt acgtcgattg gcaacgattt catatgctgc
    12601 agctgacgca gccctgcgta gcccatgccg aaatcatcca gcgccacccg aactccggca
    12661 ttgcgcagcg gacggaggat tgccaccgca gcatgagggt cgtcaatacg tcggctttct
    12721 gtcacttcca gaatcagtgt tcccggctga atgcgatagc gggttaacag ttccagcata
    12781 tccgccacca tattcgggtg catcagttgc agcgcagaga ggtttaccga caagggcagc
    12841 ataatgccgc gctcttgcca ggctgcaagc aatcgacagg actcttccag cacccagtga
    12901 ccgacggtaa ccatcagccc acagcactca atgcgatcga ttaagccatc cggcaggtcc
    12961 caactgccat ccggttgctg gatacgcagt aacacttccg cattgaccag tttaccgctg
    13021 gtcatctcga cctgtggctg taaccaaatt gcaaactgat gattttccag tgcattaagg
    13081 atatcgctct cttccgtcaa ccgcttctgg gctgcttcca tctgctgcgg atcaaagaac
    13141 tgaatctgat tcttgccttt atggcgagcg gtaaatgccg cagaaatagc gcgactgtaa
    13201 agctgttcgg cggtgagatc gccgtagaac atcgccacgc caatgctaca gtgcggacgg
    13261 agttgaatac gttcaatcgg caggcgctcg ctcatgatag tgagtacttg ctgacccaaa
    13321 gtgattgcgt gccacggttc ctgtacaccg ttggcaatga cagcaaagtc ataaccgcta
    13381 atctgcgcga ggatcatacg tggcgacagt accgatttga gtttttccac cagcgtcagc
    13441 agcagaattt ctcgttgcgc ctctttcagc acgcccgcag tatcacgcag ggtttcacag
    13501 gtgatgatca tcagcgcggt ggtttgttta cgcgcaacaa cctgctccag catctccatc
    13561 agcaaggctt tgttcggcaa atccgacacc gggaagcgca tcgcattttc gttctgttct
    13621 tcataatggc gctgcagcaa ttgctggttg aggttgtaac tgcgcaccaa cataccgatt
    13681 tcatcgtcct gatgcagacg cggtaatgcc agttggtgac caacaagctc ctgggctggg
    13741 atggcgttaa gttcgcgagc aatattgcgt aacggatgca aaatcaggcg gttaatgcac
    13801 cagctgatgg cgacggtcag gataagcgac aaaagtaagt aaatggtcac taacgttgag
    13861 agagtgctca tcacgaactt atacatacgg aaggaatccg cctgtagcac cagataggct
    13921 attggctgcg gatttgccgg acgttcgagc gagtagacgc ccagcgagat ttgaaccggt
    13981 agctcaaaca ggcgagtaac cattaccggc accggacgct ctggaataaa acttttacgc
    14041 agcgcctgga actggttagg cagcactaca tcggcacggc tgaccacgcc tgccggttta
    14101 atgctggcaa gaatagcttc cgcttcggga atatcgcctt tcaaaatagc tgaagataag
    14161 ggttcgcgga cagagcgagc aatgctttcc agttgcgtag ccgtgttata gcgattctgc
    14221 tggaccagat ggaacagcaa aacggtgcaa aaaataaaaa cgaacaccag gacaacggct
    14281 gccaccattg ccatctgctt gattgttaac gagcgactta cgcgcaaatt gtctcccccc
    14341 ggaaatccca gattgccgcc cgagtatacc cgatcgcagc ggcattaaga gaggcgctat
    14401 ctgaaaactt accagtcggc gtaaggtatc agcggctgcg gcggtaaatc catgtcaccc
    14461 tgccatccgg cggcggaata acgtacgtag agcagggaat ggctgggtgc gtaatctttc
    14521 gcctgctgga tatcaattgc cgtgccaaca aaccagttgg aagtaacacg tcgttcaagt
    14581 aatgcccgcg ccgtgtagcc gaagccctga ctgctgccgc catcgttgga ttgccgcgca
    14641 gcttcttcct gccagtcggt cgggatcaga ttcatcagcg gataacgcgg catggttttg
    14701 gtgcgtgaat gcgaccacga gccagacgcc cccagctccc acgaccagtt ttccgtgcgc
    14761 tcccgccaca tcaccggtat ggcaaacgac aggtattcct gcggactgta gtaaccgccc
    14821 tgaccgagtg agtagccact cagatctttg tcgtaatgcc agatcatgtt gttcaggccg
    14881 attgtgacgc ggcgattgtt ctggttaatg accttatagt aatagcccgt catccagcgc
    14941 acgcgccagt tatcttcgac atttttaccg gttaactggt cgccactaag cgatgcccag
    15001 acgccgtttg cttcaccttt atcgtagctc agacttagcc ccacaccgtc ggcacgtacg
    15061 ccaccccatt ttttcccggt attgctcggg gagtcttttt gcccaccaaa ggccagcaaa
    15121 gaactggaga tgggccgacg gtgggcgtta acggtgtaac ccagcggccc gatatcatcg
    15181 ctgtaactga tgccgccgac cacatccacc acgttgaagc ccatcggcgt ggtaccgata
    15241 tcccagctcc agacgtcatt tcgccagccg accgccacgc tggcaccgga atccgactgg
    15301 ctgcggttgc cgctacagtc ctgtaatgta caggtgcccc agttgtcatc ccatttgcca
    15361 tcggcattag tggagaaact gccgacgttc atattgacga aatcactgcg aaagaacatc
    15421 cgcccgtcag aatacggcgc atccacctgc aacatggtag tgtgcgcttt cagatcggag
    15481 taaccaccgg tgccgctcga accccagtaa tcgtgctcaa gggtgacgtt aagatcctgc
    15541 tggcgataga ggtccgccgc atcgctgcgc acgccgcgtt tcagccagtc atctttctcg
    15601 tcgttacggg tcagtcgggt aaaggtgtcg ttatcctgcg gacgcgtcgt agtcacaccg
    15661 gatgcgacca tggcgtcttt gtaggtttcc agcgcctgct ttggatcgcc cgcctgcgct
    15721 tcaaacttcg caccgtcgcg cagcaccatc gcgctttcca tcgacggtgg ctgagatttt
    15781 gcctgcggga tcaacttatt aaacgtctgc tgcgccgctg cggtatctcc aagctgcgcc
    15841 tgtgccagcg ccacgcgccg ctgagtgttc agcgaggtgt tatcggtggc aggcagtttc
    15901 gccagctggc tacgtgccgc cgctttgtca ccggcagcaa tatccacttc cgtcagacca
    15961 agaatggcat cggcgttagt tggctcccgc gtcaggacat tctgatatgc agtgcgggcg
    16021 gcggtgtaat cacgtcgttg ttacgcccag tcagccagcg tgaggtcaat acgcgtggaa
    16081 ggtggttgct gacgcagcat cgcttccgct tcagcctctt tgccgctttc tcgcaggcga
    16141 ttagcggttt ccagcacctg atcgctttgc agtcgattaa ccagctcctg aatattgctg
    16201 ttccactgcg cgcgcggcag gctgttgata tgcgccagcg ccgctctgtc ctggttgtga
    16261 cctgagaggt acagcccgta agcgtaaacc tgctccgggt cgttcggctt ctgctgcgcc
    16321 agattgcgca ttaacgtatc ggcctggctg cgttgtccgg cctgccagag atcctgcgaa
    16381 agtcggtaag taatccatac gctgccgggg tccagcgcca gtcgttgccg ctgaagtgct
    16441 gccgcctgcg cccatttgcc ctggttttcc agtacctctg cctgctgtgc cagacggtcg
    16501 ttttgcaggc tgcgttcgat atcatcaatg ctgcgccgct ggctggcaga gagcgaggcg
    16561 ataaacgctt cagctttttc tggcgactgc tggcggtaaa tattcgccag cccgcgcacg
    16621 gcattagtgt tgccgctgtc catccgcagg gtctgctgat aatagcgttc agcggcgggg
    16681 taatctttgc gcgccatcgc cacatcgccc agccccagca ccgcatagct gtcggtgtta
    16741 tcgacattac gtgcctgctg gaacaaccgt tctgcccggt caggattatt ggctttcagc
    16801 gcagcatcac cctgctggat cgccagccag tagcggttta ctttcagcag actgttccat
    16861 ttgtcgttgt tgctgctgtg cgggtccagt gcgagggctt tttccagatt cgccactgca
    16921 ttggcgcgat cgcctttctg agaatacgcc tggcccagcg cccccagagc ttcactgtct
    16981 ttcgggttcg cccgcaccgc ctgttgtagt tcgggaatgg ctttacccgc cataccagag
    17041 tccaccgccg ctaaaccttg cgcacgagcg cggaaagcag gatcggccag ctgtttttgc
    17101 tgttctgcca gttgcgattg cgcagccgcc acgctatcgc catcactaaa gatcgagaga
    17161 tattttttca gcgccgacac actggcatca ctgacgggca tgtctttaat ctgcccgtac
    17221 cagattttag aggccccttc gcgcccggcg ttcgatttcg ccatctgttc caggacggca
    17281 aaaccttcgt cacggcgatc gctactaaac agcaatagcg ccagattgtt ttgcaggccc
    17341 gtattgcccg gtgcatccgc attgatgcgt tttaactgat taatcgcttc gccacggcga
    17401 gccggaattt tcgccaccgt actccagtac tcgacagcaa tgtcaccttc cggcggcgca
    17461 ccgttgaaca gtttgttgta actcgccaca gcttcttccg catgaccggt agtcgcctgc
    17521 aatcttgcct gttgcagtgc ctgacgacca tccggcgtgg aaagtagcat cgtagtccgc
    17581 gacgatttat acgcatttga actcggcgct aactgcgaca gccgatcgag ctgtttttgc
    17641 gcgccatcaa tatcgccctg acgtaacaaa gaacggaaac gggcggcaac gacgtccggg
    17701 ttattcggat caataagttc cagccgatat aacgactgtt gcaccagatc ttcacgatgg
    17761 gtcgcttcgc ctaaccgaac ttgctccagc aactgttgct gagcggttgg tgctgcctcg
    17821 accatcggca tgacggccag accgagggaa agcgtgaata tgtttagtgt gaatttgcgc
    17881 attcctggcc ccagtcaggt aataactcac cttttgtcga gaagcggaaa cgatgttgat
    17941 cccagtcttg tccaaacagg gtcagcacat agttgtaata ggtatcgctg ccgggaaagt
    18001 tatcggccac gcgctggcgc tgaactgcct gcgcatcgcg gttttgtaaa aaaggcagca
    18061 tggcggcaga aaaaccgacc ggtcctttac cctgcgcttt ccccgtagcc acatccactt
    18121 tttccggcgg ataaccgttt ttctcagtga atgtcgccat cggtttaaac cggttgagca
    18181 tccgcgcttt ttgcggatcg ctgtcaggca tcatgcctac ccacatgtaa acgcggatag
    18241 cgtcgtagct gctgatcaat gttttttcgg cttttagctg ccagcctttg tctttctcat
    18301 agcgcaccca gtctggcgaa aagcctttcg gggcggtttc cagcaataaa cgttgattgg
    18361 tttcgcgcag cgtggtccac ggcgcgccaa agcgggtgaa atactgcgcc agcgtcggcg
    18421 gcatgtagct ggggttaaaa cgccagctgt tatcctcagc aaaacccact ttgcccggta
    18481 acaacatgga acccagccca ggcaccgtca ccacttcctc ccgcgcgata cgttttagca
    18541 acgcgctgcc gatgtcggta taacgctgct ctttccacaa acgccccgcc tccagcaacg
    18601 accaggccat ccagacatca ccatcggagg ccgaattgct gtccagcact tcccacttac
    18661 tgttctcttt cttgccccac agccaggcgg gcaaacgttc ttttaaagaa ccctgagcga
    18721 gattgttctg cgtccagtcg agaatattat cgaaagctgc acggtcgtta gccgccaggg
    18781 caaagaacat accgtaactt tgcccttcgg aggtggtgat tttgcgcgcg tcgctggggt
    18841 cgatgacgcg cccttcctga ctgatgtaat cctttttaaa ctgctcccag gcaggcccgg
    18901 tacaggctgc ctgaacacta aaggcagcca acaacagcat cgtcacgatt ccactacgca
    18961 acacattcat cttcaattac tcgttatccg ggttaagacg acgacgacta ataattcgca
    19021 gcagacgcca cagtacccat gccagcaata tcacactgat agccgccagc accgccagca
    19081 gaatcggatg gtttgccagc gcataccaca agcgctcgaa ccacggcaga tgacctacgt
    19141 aataaacgtc gccaacacgt aggctgttga taccggactc gcggatcacc gcgaccgaac
    19201 cgaacatggt ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt tcataaccgc
    19261 gtgggctatc tgccaacagc gcaatcacgc tgcgctggtc gttatacggc gactggaagc
    19321 caatcaccgc cgccatcgca ccggaagagg tcagcgttga ctgggtttct gccgcgcgat
    19381 cgctctcgtc cggcacaatg ccggggaacg gggtctggcg catcggtgtt ttcacccagc
    19441 tttcggtcgc ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg tccgggatac
    19501 caccgatgat catgatgtcg gcatctttgc cctgaatggt gctgccatca tcggtcaccg
    19561 tcagattaat cgccgggaag cccgtctgtg cgccgataaa accaacagta ctcagcaacg
    19621 tttccatctg tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc gacagatccg
    19681 ccatccggct gaatgggaag cccgcgttag caaaggcgcg tagatccggc atcgggatga
    19741 agtggtaata cttcgagaag tcgatggtgg aatcgtcacc aatcaccaca tgattctgca
    19801 ccggctggaa ggtaatacag ttatctaccg aaccgcctgg catcgggttc atatactcaa
    19861 agtcgaagcg cagctggttg gtcgcgccca gtttcagcgc cggaatagag acatctgttt
    19921 tgccatccag caaaccttgt aataccggaa tacgcagcag cagacggttc gcctcctgtt
    19981 tgctgctcag gttgaaggat tgcaggaact tgttattcag gctgatatcc atccgcgaac
    20041 tgtctttcac cggcggcatg gtgtagcggt aattaatatc catatcaatg ccggtactgc
    20101 gcatcaggta gagatccggc ggcaggttta gcgaaacgtt aatcgctgct ggctcaaggc
    20161 cgctggattg taactgttct tcataggttt tcagttcgcc aaatgtgacc ggacgatcgg
    20221 tacgtaccca gttcggcgca tcgtacggct tacgcggtag cagcggtttc acttcattca
    20281 ctaccacgct ttcaccacgg aacagaatgt taccctgagc gatacctttc gctgcctgca
    20341 acaggtcttt gtcgtcacga ccaaacacca ccagcagttt gacgtaagga ttctgcggat
    20401 ggttaatcat ctcaatcacc ggggctttta ccgccggatg atcgcgcagg aagtccggcc
    20461 gtttgtcgtt ggttgcaaag acaatggcat tgcgatccgg caattggtta tagagcaccg
    20521 ggaagttctg cccacgccag ccagaacgcg aaccaaacca cgaggcgaca atggcagatg
    20581 cttgttgcag cccaacatcc ggcgcacccg caaagaccat cggcaaggtg ttagtacggt
    20641 tatcgcgcgg gtcaaagaat ggcaccggga agtgtgacag gtcattcttc acattcaggg
    20701 tctgataggt cagatccagt ccactgctcc gcccaacatc cagccaaagc gtggtgctgg
    20761 ccgggttttc gcacacgtcc tgataatggc cgacaaactc cagccgcaca cggttgaagt
    20821 cggtaataaa cagtggatta atgggcattt gcgccagcgt ttttttaccc aactgttctt
    20881 tggtcactgg cagcacgccc atcagctcat cattgagata aacctttaac tgcgactgga
    20941 caggcagtaa cgatggcgat ggcgtgtatt cgaggttgag catcgccttc gtcaccactt
    21001 catcgctgcg cataccaaac tcaatgctgc cgttcgggtt aataccacgt agcaccatgc
    21061 tgcccggcgg cggcgcgatt tgcgcaaagg tcagcttcac atcacgcgaa ggaccgttct
    21121 gcgccacgat gggggcatca gctccctgct cgcctgacat cacttgcccc acctgcggat
    21181 tttgttccgt ctgggcggct acagctggct cagcattgat cagtggttgc gttgctggcg
    21241 tcgcctgcgt catgaaagag gggaacgcac tcatccccat agccactgca caaatccaga
    21301 atagttttct tttcatcgcg ttatcatcat tgttgagcca aagcctgatc cgatggttgt
    21361 gccgtttcgc tccgctccgg gcggcgcgga ataaacgata caacccagga aaccagagaa
    21421 gtcagcacac ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag atggcggtag
    21481 ccacggaagc cgagcttcag aatatccagc agactttcca gcggcttatc ttccgggtag
    21541 ctgtcctgcc agagcgccca tgtatccgca cgggcaaacg tacactgcac aaaatcgata
    21601 tgttgctggg tggtgagcgg cattaatttc agcccaactt cattacccat cacgcgcgcc
    21661 acctgggtcg ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt cactttctgc
    21721 ccttccagaa tctgcgcctg accgttgatc ttgatcccca aaccaccgtc ggagaaatcc
    21781 tgaacggtac acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg catcgtcatc
    21841 tccacgcggt gcgatcggcg tacctgtttg ctttctaccg ataccgcaac tgcgccgcca
    21901 agaacaatca ggttgtagaa cacccacacc atactgacga ccacggtgag catctcggtt
    21961 ggcgggccat agaagtagcg ccagatgcct accgcaacgc ccaccaggtt gagtagaaca
    22021 aggaagatgt agggccgcga gatcacccag tcgacgtact cttcttccac cagtccacct
    22081 ttggcggtga cgttaaattt gcctttgtgc gggttaatca gcgccaccag cgtcggtggt
    22141 gcgatatacc acgccagcac cgtttcgtag atttcactcc agaaagagtg gcgatggatc
    22201 ttcgagttgg tcaggctggc atggatcata tgcggcagca cgaatagggc gatcatcaac
    22261 gctggcgcat agatgatgta ggcatgaagc agcaggaacg ccagcggcgc agtcaggaag
    22321 atcagccgtg gaatgcccga caagaagtgg aacatggcgt tgacgtagca cagccgctga
    22381 gcaaacttca gtcctttacc ggtgagcggg ttatcgagac ggaagatttg taccatcccg
    22441 cgcgcccagc gaatacgctg accgatatgc gccgacagac tttcggtcgc cagccccgcc
    22501 gcctgcggaa tacgcatata cgcggaggta tagccacgac ggtgcaaacg cagagaagta
    22561 tgcgcatctt cagtcacggt ttcgacagca atgccgccaa tttcatccag cggcttacga
    22621 cgaatcaccg cacaggaacc gcagaagaaa gtggcgtccc acatatcgtt gccatcctgc
    22681 accagaccat agaacagcgt gccttcgttc ggcgttttac gaaaacgtcc caggttgcgt
    22741 tcaaacgggt ccggcgagaa gaagtggtgt ggagtctgca tcatcgccag ctgtttctct
    22801 ttcaggaacc agcccatggt catttgcagg aacgatcgcg ttggtacgtg gtcgcagtcg
    22861 aaaatcgaca cgaactcgcc tttggcatat ttcagcgcat tgttgatgtt gcccgctttc
    22921 gcatgttcat gagtggtgcg ggcgatatat ttcaccccca cgttttgcgc aaactggcga
    22981 aactcttccc tgccgccgtc atcgaggatc cagatgttca gtttgtcttt cggccagtcg
    23041 atacccagcg aggcgtaaat ggtatttttc accacattga gatcttcgtt gtaagtcggg
    23101 acaaagatat ccactgacgg ccacagcgac atatccttcg gcaatggcac cggctgacga
    23161 ttcagcggcc atactacctg gaagtagccg agcaccagca caatccacgc gtacgtttca
    23221 gcgaagagca gaataagccc gcacaccagg ctgaccggat cgtcccagtt cagcgtagag
    23281 gtgtaacgcc accagatata acggcaagaa acggtcagcg acagcacaat caacattagc
    23341 gccgagaagc gccccggcat ccgccgtacg atcagcgcta ccccccacag cagcatcagg
    23401 aagataaact gcgccagcgg gttaaacggc tgagtaacgc agattaacgc cagaatcagc
    23461 gagaaggtga cgatgatacc gaggatcaac cgccgcgccc cggcactcaa atgaccgagc
    23521 tcttttttct catccagatg ctgtgtttta tggctaacgc gctcaggcag ctcgttcatc
    23581 cattgatggt aacgtccacg aatattttgc agacctgaaa atgcccgctg cgcggtttcg
    23641 gcgtttcttt gcgcgatgca ccgatcaata accagcatgt ttgaatgaga taacggaccg
    23701 ggtccagcgg acgcgaggcg ttgatatgcg tatacaggtt tttatgttct gcgcgaatac
    23761 gctgccagcg cgggtgctcc agcggaataa aaatccaggc caggatcatc cagaaacagc
    23821 cgagcgtcgc gctgaaagcc gacgcaccgt gacgacgata atcgcgataa cgcccgataa
    23881 gccgcgcgtt gaccggcggg ataagcaacc accgggtcag gatactcatg atgcactccc
    23941 gactggcgtt ttcagcccgg agtagttcaa caggcaccag ttcgccagcg tcagtatctc
    24001 ttcagccgcc agcgcatcac tgcgatattc acctactggt tgcttagccg ccaggcattc
    24061 agccatcgct tcatcacgat gaatgagcat cggcagtaat cggcgctggc tttgcaacca
    24121 aagctggtaa atatcgtcct gaacctgact gccaatacgg aagtcattaa tcaaaatatg
    24181 tgcgccatcc ggcagcgctt gctgatgcag tcggatatgg cagttggcat ccacattgac
    24241 gattgccagc gagtgctcgc acaaactcag cagctggtgg gttatctgcg aggcatcacg
    24301 cggtaagtcg attaaaatcc actggtaacg cccgttggct tttagttgct gtaagccgga
    24361 gcaaatatcg ctcagccggg tttgccagtg ctgtggattt tcttgttctt caatggataa
    24421 ctgaccaaaa ggcagcaaat cgagctgcga ggtgtagcgc aacccagcgt cacgccagtc
    24481 ctggccatcc agcatcgctc tggcccagcc ctgacggtgg gtaaaatcaa cattaaatga
    24541 caggcgcaac aagttgtccg ggcaggcatc gaccaccagg acattttctc ccaacatttg
    24601 taatgaccag gctaatgcgg cggtgatggt tgttgtcccc acgcctcccc gcaccccctg
    24661 caatcccagt acggccattc accaatcccc tacttttgtt gcgcaaactc tgccagcaac
    24721 ggccagcgtt ttaatgccgc ggccaactgt tcgcgttggg aaatatcggc ataatcaata
    24781 tcaggcagtg aaaatgcctg ctttaacgcc acaatatcat tctggaagat atagcctatc
    24841 gcgggatcag gcagagtatc tggttcattg ttattcattt tgcttgatcc ctatgaaata
    24901 accgtaccgt tcatgagaca attctaaccg tgagcatttc gccggtttat tgtttacggc
    24961 gagacgggaa tttgctcact ttttgacttt ccgttacgca acagaaaaag ttaaaaacga
    25021 aaagactaaa atcacacgtc ttgtgacctt ctaaattgat tcacatgcta aatctgataa
    25081 gttttaattt caatggtagg tttatttctt agctttcgct agtaaactga taatcagaca
    25141 aaatggtgac ataagggaca ttgtggaccc tgtattctct atcggtatct catcattatg
    25201 ggatgagctg cgacatatgc cagcaggcgg cgtctggtgg tttaacgtcg atcgccatga
    25261 agatgctatc agtctggcga atcaaacaat tgcatcccag gctgaaaccg cacacgtcgc
    25321 ggtcattagc atggacagcg atccggcgaa aatctttcaa ttagatgatt ctcaagggcc
    25381 ggaaaaaata aaattatttt caatgctaaa tcatgaaaaa ggtctatact atttggcccg
    25441 tgatttgcag tgttctattg atccccataa ttaccttttt attcttgttt gcgcaaataa
    25501 cgcatggcaa aacattcctg ccgagcggct tcgctcatgg ttggataaaa tgaataaatg
    25561 gagcaggtta aaccattgtt cgcttttggt aattaatccc ggaaataata acgataaaca
    25621 attttcattg ttgcttgagg aataccgttc a
//
LOCUS       DADLSE010000047        24961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLSE010000047 DADLSE010000000
VERSION     DADLSE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.047"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN692_001928"
     CDS             complement(572..1348)
                     /gene="sseB"
                     /locus_tag="JN692_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7264250.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGAAAQGE
                     AVVEDSVLDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSGQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN692_001929"
     CDS             complement(1490..2773)
                     /gene="pepB"
                     /locus_tag="JN692_001929"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7264251.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN692_001930"
     CDS             complement(2832..3032)
                     /gene="iscX"
                     /locus_tag="JN692_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7264252.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN692_001931"
     CDS             complement(3044..3379)
                     /gene="fdx"
                     /locus_tag="JN692_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7264253.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN692_001932"
     CDS             complement(3381..5231)
                     /gene="hscA"
                     /locus_tag="JN692_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7264254.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKTLAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN692_001933"
     CDS             complement(5248..5763)
                     /gene="hscB"
                     /locus_tag="JN692_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7264255.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN692_001934"
     CDS             complement(5859..6182)
                     /gene="iscA"
                     /locus_tag="JN692_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7264256.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN692_001935"
     CDS             complement(6199..6585)
                     /gene="iscU"
                     /locus_tag="JN692_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7264257.1"
                     /translation="MAYSEKVIDHCENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN692_001936"
     CDS             complement(6613..7827)
                     /gene="iscS"
                     /locus_tag="JN692_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7264258.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN692_001937"
     CDS             complement(7939..8427)
                     /gene="iscR"
                     /locus_tag="JN692_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7264259.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN692_001938"
     CDS             complement(8697..9437)
                     /gene="trmJ"
                     /locus_tag="JN692_001938"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7264260.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9556..10359
                     /gene="suhB"
                     /locus_tag="JN692_001939"
     CDS             9556..10359
                     /gene="suhB"
                     /locus_tag="JN692_001939"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7264261.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10504..11358
                     /locus_tag="JN692_001940"
     CDS             10504..11358
                     /locus_tag="JN692_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7264262.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     APWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11549..12829
                     /gene="csiE"
                     /locus_tag="JN692_001941"
     CDS             11549..12829
                     /gene="csiE"
                     /locus_tag="JN692_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7264263.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12821..13960)
                     /locus_tag="JN692_001942"
     CDS             complement(12821..13960)
                     /locus_tag="JN692_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7264264.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGCEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYPYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN692_001943"
     CDS             complement(14120..15010)
                     /gene="hcaR"
                     /locus_tag="JN692_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7264265.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSTEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15146..16507
                     /locus_tag="JN692_001944"
     CDS             15146..16507
                     /locus_tag="JN692_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7264266.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWVLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16504..17022
                     /locus_tag="JN692_001945"
     CDS             16504..17022
                     /locus_tag="JN692_001945"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7264267.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17022..17342
                     /locus_tag="JN692_001946"
     CDS             17022..17342
                     /locus_tag="JN692_001946"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7264268.1"
                     /translation="MNRIYACPVEDVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17339..18151
                     /gene="hcaB"
                     /locus_tag="JN692_001947"
     CDS             17339..18151
                     /gene="hcaB"
                     /locus_tag="JN692_001947"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7264269.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTCSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALEQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18161..19363
                     /locus_tag="JN692_001948"
     CDS             18161..19363
                     /locus_tag="JN692_001948"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HAY7264270.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIAIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            19460..19882
                     /locus_tag="JN692_001949"
     CDS             19460..19882
                     /locus_tag="JN692_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7264271.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19930..20802)
                     /locus_tag="JN692_001950"
     CDS             complement(19930..20802)
                     /locus_tag="JN692_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7264272.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGNKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20814..21896)
                     /locus_tag="JN692_001951"
     CDS             complement(20814..21896)
                     /locus_tag="JN692_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7264273.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAKGRGAKRIIGVDMLPER
                     LAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTADWGR
                     VVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAITHRF
                     SLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21929..22927)
                     /locus_tag="JN692_001952"
     CDS             complement(21929..22927)
                     /locus_tag="JN692_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7264274.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVLID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIK
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22952..24463)
                     /locus_tag="JN692_001953"
     CDS             complement(22952..24463)
                     /locus_tag="JN692_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7264275.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     KGASKSTLIRKLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24486..>24961)
                     /locus_tag="JN692_001954"
     CDS             complement(24486..>24961)
                     /locus_tag="JN692_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7264276.1"
                     /translation="AFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKL
                     IISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAV
                     VDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5881 a         6980 c         6229 g         5871 t
ORIGIN      
        1 tcagacaaat ataaacaaat cctgctcaaa attaaaaatt ctaaccggta aaagatatta
       61 cttaaacatg taaatttact ttcctttaaa aaacaaaaaa ccgccaaaat caggcggtta
      121 tttgttgctg gtccggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
      181 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
      241 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
      301 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
      361 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc acatggaccg cacaatgaag
      421 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
      481 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
      541 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
      601 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
      661 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
      721 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
      781 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
      841 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
      901 gctctgcaac ttccgataat atcagcgatt caccgccttc caggatttcc tggccgctca
      961 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
     1021 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     1081 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
     1141 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcacac
     1201 tatcttcaac cacagcctcg ccctgcgccg ccgcaccagg cacccagacg gtggattcca
     1261 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     1321 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
     1381 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
     1441 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
     1501 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
     1561 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
     1621 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
     1681 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
     1741 gacgccagaa tggttcgttc tcttgtgagg cactcgccag taaacgaccg gcaagcgcat
     1801 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
     1861 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
     1921 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
     1981 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
     2041 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
     2101 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
     2161 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac accggtgctt
     2221 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
     2281 gcccgacagt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
     2341 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
     2401 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
     2461 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
     2521 gcgtgccttt cgggcctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
     2581 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct
     2641 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
     2701 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
     2761 tcgcttctgt cattttagtt atccttattc gtttaacagc aaaacgggct acccccaggc
     2821 agcccgttca ttcactcggc ttcgtccagc cagactaaca aaatcgcttc gaggattttc
     2881 tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata
     2941 tcggtgaatc gaaccgtttt cggatcaaga tcgggatacg catcgtacag tgcttcgcca
     3001 atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc tcacgcgcat
     3061 ggttgatagt gtaacgcggg atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc
     3121 aacttaaacg gctttccggc tccagtcccc aggctttgtc cagcatgtcg tcttcctgct
     3181 ctgagctttc cggcagtgag tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag
     3241 cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa
     3301 tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc tgatgaggca
     3361 aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag cgcacgacga
     3421 accgactggt ccatgcggcg agcggcgaaa tcctgggttt gtttgtctac gtttttaatc
     3481 gcttgttcga tggcgtcaac atcatcgccc tgcgccactt cactcaggtg agcggcagca
     3541 ttgtcaatga cctgacgctc tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg
     3601 tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat acgggctttt
     3661 acgtcctgct cggcatagct cattgagtct ttgatcatcg aagcgatttc gctatcggtc
     3721 agaccgtaag acggtttgac ctgaatagac gcctcaacgc cggtggattt ctccatcgcc
     3781 gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca
     3841 gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc
     3901 agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc tttaaaggtg
     3961 gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac tttctccacc
     4021 aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag cagcatttcg
     4081 ctgtctggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat agcgacgact
     4141 ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc
     4201 accagcggca cgcgagtaga accgcccacc atcaccactt ccagcacttc gtcagcttct
     4261 acacccgcgt ctttcagcgc gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg
     4321 atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac gttaacggtc
     4381 acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag cagttcacgc
     4441 tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta atccgccagc
     4501 agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag cacttcaaac
     4561 acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag gtcataaaca
     4621 gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg
     4681 ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt gccctgacgc
     4741 tgggcatcgt caaagtacgc cggaacggtg ataactacac catccagctc gcctgccagg
     4801 gcttcagttg cccgcgccgc cagtgttttg aggatgtccg cagaaacgcg caccgggttc
     4861 agtagccccg ccgccgtttc aatcatcggc aggccgtttt cgctggcctg gaattgataa
     4921 ggcagatgcg gatagcgttg ctggatatca gccagcgagc gccccatcag gcgtttaaca
     4981 gaactaatgg tgttggcggt gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa
     5041 tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc atggtcggct
     5101 aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg agttggttgt gcccaggtca
     5161 ataccagccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc actaatttgt
     5221 aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt cgagttgttc
     5281 ggcactgctc cgcagtttat cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc
     5341 ccacgcctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa acatcttttt
     5401 cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga tctcgtccag
     5461 ctcttcgcgc agctccaact gttccatcag gaacgcggtg tcgcgcacag tatgctgctc
     5521 gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc gcgcgcatta acggatgacg
     5581 cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga gttgttccgc
     5641 ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct gaaaacgcag
     5701 gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc
     5761 cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa aacaaacccc
     5821 acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg
     5881 caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt caggccttct
     5941 tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc gaccacgact
     6001 ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac aaattccagt
     6061 acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc aaaccctttg
     6121 ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag tgtaatcgac
     6181 ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg caatggcggc
     6241 tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca gttcaagttc
     6301 ttcagcaata tcggtgtttt tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca
     6361 ttcggtgacc agggagctgg aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc
     6421 gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcattacgt cgccacaggc
     6481 cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt
     6541 acgcggattc tcgcaatggt cgataacttt ttcgctgtaa gccattataa attctcctga
     6601 ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc cctgcttgta
     6661 catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa ctaactcgat
     6721 ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga tagagctatg
     6781 tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga ggcttgctga
     6841 cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca gcgactcacc
     6901 ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt
     6961 caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca ggccgcgcag
     7021 acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcttcgc ccattccgac
     7081 gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat
     7141 ttgcgcttcg atgcgtacac gcggtttacg acgcacatac agcgcaccga tacctttcgg
     7201 gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc tcaggtcgat
     7261 tggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac gagcacggca
     7321 catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta cgtgcatgat
     7381 ggaaacaagg atggtgtcgt cacgcatcgc cgcttcgagt tctttcaggt cgataatacc
     7441 gttacgctgc ggtgccaggt aggtgacttc gaaaccttcg cgctccagct gacggcaagt
     7501 atccagtacc gctttgtgtt cggttttgct ggtgatgatg tgcttgcctt ttttctgata
     7561 aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag aggtaaagac
     7621 gatttcacgc ggatcagcgc cgaccagatc ggcaatctga ttacgggcga tatctaccgc
     7681 ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa aggttccgtc
     7741 catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg tggttgcgga
     7801 gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca ctcaatgcaa
     7861 ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc actccggcct
     7921 gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt
     7981 gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct ggttattaac
     8041 cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca aatcacgcca
     8101 cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct gacaacgggt
     8161 ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc tgctggcatc
     8221 tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac catttttacg
     8281 cagacgggaa aacagttgtt ccagataaga aagggaaatt ccctgacgtt cggaaatatc
     8341 agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg cggtcacggc
     8401 atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg
     8461 ggttttttat tgtaaaagtg ggggtattgc atagcagggt caagtctgac attcccgagt
     8521 aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca gtgccatttt
     8581 tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc cgacctacga
     8641 ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa ccactgttat
     8701 tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc
     8761 aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt catcacctgc
     8821 cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag atgaccataa
     8881 aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca
     8941 ttttcctgag ttgccagcca ggccatacgc acttcatagg caataacctg aaccgccatt
     9001 gccaggttca gcgaactgta ttccgggttt gccgcaatcg cgacatgata atggcatttc
     9061 tgcaactctt cattggtcaa gccgacgcgc tcacgaccaa acaccagcgc caccggggta
     9121 tttgccgctt cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc
     9181 agcgtgcggg agcgtgcgct ggtgcccacc accaggctac aaccggctaa cgcttcgtcg
     9241 agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc
     9301 gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa tcccattgtt
     9361 ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt
     9421 cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa
     9481 acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc
     9541 cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg
     9601 ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc
     9661 agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt
     9721 cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact
     9781 gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt tatcaaacgt
     9841 ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg
     9901 gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg
     9961 aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat tctggcgacc
    10021 ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaagctg
    10081 ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc
    10141 gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga tttcgcggca
    10201 ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg tggtcataac
    10261 tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc catactggcg
    10321 aacatgcgtg acgagttaag cgacgctctg aagcgttaat gactcaggcg ggtgatatca
    10381 ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct
    10441 ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag
    10501 gttatggcac tgccagtgaa caaacgcgtt cccaaaattc tgtttattct ctttgttgtt
    10561 gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta tcctgacgat
    10621 aaaatttacg gtcccgcccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt
    10681 actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca
    10741 accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt
    10801 tggttacctg agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca
    10861 aaaggcacgc cgtcccaggc cggattgctg gacgatacgc aaagcgccat caatgtggtg
    10921 cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg
    10981 gcgaatattc ttgctgttat tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc
    11041 gactccacat ttgcctctta tgcaaccatc gccaaccaga tgatccccgg cagtggctac
    11101 ttacttgatg agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt
    11161 ttactcattc acggtaaagc cgatcacgtt atcccatggc agcacagcga aaagttgtat
    11221 agcctggcaa aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct
    11281 ttttccgatc gtcacggcaa tgtttatcgc gaacagatgg tggactttat ccttagcgcg
    11341 ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa aaccacctta
    11401 agacatgcta atccactggt cagaacagtt taaaatgaga aaaattctgt gacgcttgcc
    11461 aacatttctg atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt
    11521 atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct
    11581 ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc
    11641 caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat
    11701 cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga ctggttatga
    11761 cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct
    11821 acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc ccgccctgaa
    11881 gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc
    11941 gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaatcgcgcg atattcattt
    12001 cctgtgtctg tatctgcaat attgtttgct gcagcaccac gctggaatta cgcctcagtt
    12061 taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat
    12121 tggacgccac tggcagcgtc gggcgctcca gcatgtaccg cctgatgagc cactgtttat
    12181 ggcattactt ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga
    12241 cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt
    12301 tcgtttttat gatgaacagg ggttatgtga tcagctttat atccacctcg cccaggcgtt
    12361 aaatcgcagt ttgtttgcca tcggtattga taataccctg ccggaagagt tcgccagact
    12421 gtatccacgc ctggtacgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg
    12481 cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg cctggctaat
    12541 gcaggaaaac gatctgcatg aaaaacagat tattctactg accgggaatg atagcgagcg
    12601 agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca acattaagca
    12661 tatgtcggta aaggcatttt tgcagacagg cgctccgcgt ggcgcggcac tgattattgc
    12721 gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgct
    12781 gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt
    12841 tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac acgccgtggc
    12901 ccagatatgg atacaggaaa ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg
    12961 cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgca accctggcga gcagcaatat
    13021 aacgcatggc ggccaggtgg cagaccgtga aggtgccgca atgcagaatt tgcaccacta
    13081 tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg
    13141 ccgagatcaa cagcatatcg cgagcactac aacggcggaa aagtttatta ctcagcgcaa
    13201 agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt
    13261 agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca
    13321 ataaacaaac gcaggccaga aagcgccagt tctggcgaac cagcgccaac cacgcagacc
    13381 aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa
    13441 agccgagcag catggatgcc acgcccaacg tcaacagcac gaggatcacc cgataatcaa
    13501 acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca
    13561 gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg
    13621 tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg
    13681 ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tggcagcagt gccagcacgc
    13741 gcaaggcgga aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga
    13801 gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc gttaaaccaa
    13861 tccctttaag ccagacgctc cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga
    13921 aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg
    13981 cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg
    14041 cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc gcgacgcatg
    14101 ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc acaatggcga
    14161 tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt aaagcaatgg
    14221 aaggcacatt tccggcaatg ggacgaaaaa caacctgccc ggtattaaaa ttattcatat
    14281 aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc accagaatat
    14341 tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaaccac gctttaacga
    14401 tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg ccatccagtt
    14461 gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac accaccacta
    14521 atggttcgtc aaaaagctcc agataatcaa tctccgtgct ataaacagga tggcgcatta
    14581 agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg attaaactca
    14641 ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact tttggcaata
    14701 aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc tgaacaattt
    14761 tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg tcctggagaa
    14821 aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt aacggaacac
    14881 cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag gtatgcagtt
    14941 tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc
    15001 gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag aaattaacta
    15061 tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga aggtcaatac
    15121 atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca tttaccaact
    15181 gattgacacc caaaacggtc gggtcactcc gcgtatttat accgacccgg acatttacca
    15241 actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg aaagccagat
    15301 cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcagttg tcgtggtgcg
    15361 ccagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg ccatgcgtgt
    15421 gagttatgca gattgtggca acactcgcgc ctttacctgc ccgtatcacg gctggtctta
    15481 tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc cacaagggtt
    15541 gtgtaaatcc cactgggtgc taaacgaagt tccttgtgtg gagagttata aaggactgat
    15601 ttttggcaac tgggatacca gcgcaccggg cctgcatgat tacctgggtg acattgcctg
    15661 gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca
    15721 aaagtgggtg atcaactgta actggaaatt cccggcagag cagttcgcca gtgaccagta
    15781 tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag atgatggcag
    15841 cgataagcgc ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc
    15901 gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa aaccggatgc
    15961 taatgtctgg gtcgatggcg cagtttcaag ctattaccgc gaaacctatg ccgaagcaga
    16021 acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata tattccccac
    16081 gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg gccctgatca
    16141 agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag ttaaagccgc
    16201 ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc aggatgactc
    16261 ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca acagcaaact
    16321 gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc ctggcattac
    16381 taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg ccgatcttct
    16441 gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc aggaggtgat
    16501 gaaatgagtg cgcaagtttc actggagtta catcaccgca ttagccagtt tctctttcac
    16561 gaagccagtc tgctggatga ctggaaattt cgtgactggt tggcacagct cgacgaagag
    16621 attcgttaca ccatgcgcac cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc
    16681 cagccgccga cgacctggat tttcaatgac accaaagacc agctggagcg gcgaatcgcc
    16741 cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcactcg tcacttaatc
    16801 agcaactgcc aggtaagcga aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat
    16861 ctactttatc gggcacaaaa agagcgcgat gaaacattct atgttggaac gcgtttcgac
    16921 aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat cgtcctagat
    16981 caagcggtga tcacttccca taacctgagt gtactgttct gatgaatcga atttatgcgt
    17041 gtcccgttga ggatgtgccg gagggtgagg ctctccggat agatacctcg cccgtcatcg
    17101 ccttgttcaa cgtgggcggc gagttttatg ccattaacga tcgttgcagc catggtaatg
    17161 cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg ctacacgccg
    17221 ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca
    17281 cttatccagt acacgttgaa ggtggtgaca ttttcatcga cttaccggag gcgcagccat
    17341 gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat tagggctggc
    17401 gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac tgtcggcggc
    17461 aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt
    17521 gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgacttgtt ccggcaagct
    17581 ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg ttaatactcc
    17641 cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac tcggttacct
    17701 gctgggcgca aaagcctgcg ctccggcgtt aatcgccagt gaaggcagca tgattttcac
    17761 actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg ccagtaaaca
    17821 tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa
    17881 tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc tcgagcaaag
    17941 tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt taccgctgca
    18001 atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat cgcggcgcaa
    18061 taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat
    18121 tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa aaacgatcat
    18181 tattgtcggt ggcgggcaag cggcagcaat ggctgcggcc tcgctacgcc agcaagggtt
    18241 caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac gacctccact
    18301 ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac ccgctaactg
    18361 gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga
    18421 cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc tttttatagc
    18481 aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac gctgctttac
    18541 cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg aacggtcagt
    18601 cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca cgcagcgcag
    18661 atgtaaggtg acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc
    18721 cgtgcaacgc tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa
    18781 tgccattgaa catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac
    18841 gcttcaggct gatgtggtga tttacggtat tggtatcagc gccaacgagc aactggctcg
    18901 cgaggccaac cttgatactg ccaatggcat tgccattgat gaggcttgcc gcacctgcga
    18961 tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca
    19021 ccgctgcgaa agctgggaaa acgccaataa ccaggcgcaa attgccgctg ccgcaatgtt
    19081 agggctaccg ctaccgctac tgccgccgcc gtggttctgg agcgatcagt acagtgataa
    19141 cttacagttt attggcgata tgcgtggcga tgactggctt tgtcgtggca acccggaaac
    19201 tcagaaggcg atttggttta atctgcaaaa cggcgtgctt atcggtgcgg tcacgctgaa
    19261 tcaggggcgt gagattcgcc caattcgcaa atggatccag agcggcaaaa cgtttgatgc
    19321 gaaactgctg atagatgaga acatcgcgct taaatcactg taaccaggat aattagcgaa
    19381 tatctcaatg cctggggcgt ggcgaggtgc aagagtgtgt attacgttta aatcacatta
    19441 tcttgcaaag ggattggtta tgaacacact acgttatttt gattttggag ctgcccgccc
    19501 cgttttgtta ttaattgccc gtatcgccgt ggtcttaatt ttcattattt ttggttttcc
    19561 caaaatgatg ggctttgacg gtacggtcca atatatggcc tcgttgggcg cgccaatgcc
    19621 gatgctggca gcgattattg cggtagttat ggaagtgccc gccgcgatat taatcgtgct
    19681 tggctttttc acccgtccgc tggcggtgct gtttattttc tacacgctgg gtacggcggt
    19741 gattggtcac cattactggg atatgaccgg cgatgcggtt gggccaaata tgattaattt
    19801 ctggaagaat gtcagtatcg ctggcgcgtt cttgctattg gcaattaccg ggccgggggc
    19861 aatttctctc gatcggcgtt aggaaagatg ccggatgcgg cgtgaacgcc ttatccggca
    19921 ttttataaat tacagccact ctacccgcaa cgacatctct gaggttgttg attcccctgg
    19981 tgctagcgca atgagatcac ccccttccgg gcgatgatga tcatccggcg catgactcat
    20041 cggttccaga cagaagaaat caaacgcata tcctttatca aacgcagggt cggaaacaaa
    20101 gatgaaataa cacggtgcag gtggcgtcgt ttccatgatg atggcataac cttcctgcgg
    20161 ctgctcgatg cgggcctgac cattccatcc ggcaaaacca ttgttcaccc actgacgcgg
    20221 caacggcgcg agctggttaa aatctagttc ctgcggtagc tgctcgcaaa actcacccgc
    20281 cagccactgc tcccgttcca gccagtaacc gctcgcctgc gcctgaatcc gcgtttgtgg
    20341 tgacaacgga aaataaggat gccagccggt accaaatggc agcgtctctg ccccttgatt
    20401 ggtgacagag agcgtcaccg tcagcgtatc cgccgttaaa tgaaacgcct gacttacccg
    20461 atagtgatag acaccgctgc gatgttcata caccagacac aaactatcat cgctgtgcga
    20521 gacacattgc cactcgccca gccagccatc gccgtgtaga tagtgcgcat cccactcaac
    20581 attcggttgc agttgatact cacgcccctg ccagacaaaa cggttgccgc tcacccggtt
    20641 tgcaaatggc accagcggaa aacatgaggc atcggttgcc acaccgcttt tgttaccagg
    20701 acgtaagagc ggcgtcgtat cgcgccagaa gccttcgatt acgccgccct ggtcagaaac
    20761 gtccagcttt agcgacccgt gggataaggt atagatggtc atccgctaac tccttaatcc
    20821 gggaagttaa tcacaacttt cccgcatttg ccgctcgcca tcagcacata ggcatcacct
    20881 gcctgttcta gcgagaagcg atgggtaatg gcgttacgcg gccacagctt ccagtcagtc
    20941 aaatcgtggg cgcatttttc catatggaac agactggtca cccaggagcc gataatccgc
    21001 cgttgatggt gcatcagatc agcgctgacc tcgaattcca cttttccggt ttcaccaatg
    21061 taaaccaccc gcccccagtc agcagtggat tgcagtgcca gcaagcgacc tgcggcatta
    21121 ccggaacaat cgagcgcaac atccgcgcca ccgtgggtaa gttcagcaat tatctgcggc
    21181 agaccttcgg tggttgctaa atagccgtga tccatcaccc ctaactgttt tgccatcgcc
    21241 agacgttccg gcagcatatc aacgccgatg atccgttttg caccgcgacc tttcgccatc
    21301 atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac ttcgccgcgc
    21361 aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc ttcgtagctc
    21421 agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc ggcatgaccg
    21481 ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat aggaaagccg
    21541 cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac gcggtcgccc
    21601 tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg ttcatgaccg
    21661 ttgataaatc cctggtataa cggtttatcg ggtgccgccg ccgtggcacg gtgttgatga
    21721 tagatatagt ggacatcgct tccgcaaatc ccggaggatt tcattttgat cagtacctgg
    21781 ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggttgaatt tcctggtaaa
    21841 taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat tttcatccct
    21901 gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgctt cgctgggtca
    21961 gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgtaccacc tgctggaaaa
    22021 aggagttaat accgagcagc accagaccgt taccgattag cgtaatcacc agcacaccaa
    22081 gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg accacgaccg
    22141 cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccggaa ccgaggcgtg
    22201 ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag ataagaatgc
    22261 gcactcgacg aactttaata ccgcacaact gcgccgccgt ggcattaccg ccaacggcaa
    22321 aaaccgagcg cccgaaggcg gtttttcggc taatgaacac aaacagcgca aacaacacca
    22381 tcatgatcag cgcggatact ggcacaccga gaaattgtcc gcccagccag tccagcacct
    22441 cgttttcgtc aatcagcact ggcagtgcgt tggtcataaa cagccccatt ccgcgcaggg
    22501 cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg cgcagcaccc
    22561 cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca atcgccaacg
    22621 gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc atcggcccaa
    22681 cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg gcaatcccaa
    22741 tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag ccaggcgcgt
    22801 tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc tcattaatat
    22861 ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt aatgacgaag
    22921 cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg gcggacatca
    22981 gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtgccgtgc tgtaataaca
    23041 ggatgcggtc acacaccagc ggcaactcct ccacttcact ggagataaac accacgcttt
    23101 ttccttcggc agccagctca cggacaatac ggtaaatctg ctgtttggct tcgatatcga
    23161 cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc caacgaccga
    23221 tcaccacttt ttgctgattg ccaccagaaa gcgtgccgat gggtgtttcg ctactggcag
    23281 ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg gaccattgca
    23341 gcacaccgtt ggtgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca
    23401 gccagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca
    23461 gcatgtcgcc gtaatcgggg cgcatgattt tctcgccgtt gataacaatt tcgccctgtt
    23521 catacgtctc cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcaggc
    23581 cagcaatgcc aagcacttcg ccacgacgta gcgtaaagct gatatcctcc agcttgggct
    23641 tatggcgtaa cgcacggact tccagtacgg cctgatccac aatttcctga ggggcaaccg
    23701 gcgcaatatc aacgtgatca cgccctagca ttagcgacac aatatgatgc gtggaggtgt
    23761 tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa
    23821 tgcggcggat ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca
    23881 tctttttcac cgcactgatc accagttcaa cttctgcact ggcaagcgag ctggtgggtt
    23941 catcaagaat gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc accagttgct
    24001 tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg cccagcgcct
    24061 gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg ttgcggcgag
    24121 gccattgacc aaggcagagg ttttctgcca ccgtcagccc ttccaccaga cttaattcct
    24181 gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct tcgtcacctt
    24241 ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg gcgttcgctg ccggtaagct
    24301 ttcgaatgag agtcgatttg ctcgcgcctt ttttacctaa cagcgcacga acttcgcctt
    24361 tattgagcgt gaagttaacg ttatccagcg caacgacgcc ggggtaacgc ttatttcctg
    24421 ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc tctgcgaatc
    24481 agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccatc ttccgttttg
    24541 gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt attgataacc
    24601 ttcaatgttt gcgcgaaaac agcattgccc attttcttac cggaaatatc cactaccgct
    24661 ttcagcacct gattgttttc cagctcctga gcaatttcgg ttgtcatatc cgaaccgaaa
    24721 acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc gagtgtcgca
    24781 ccgcccgact cccccataat ggcgttgaga tccggcgtgg agataatcag tttttcacca
    24841 acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg cgcgccggga
    24901 acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac ttcaaaggct
    24961 t
//
LOCUS       DADLSE010000048        24752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLSE010000048 DADLSE010000000
VERSION     DADLSE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.048"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(47..4663)
                     /locus_tag="JN692_001955"
                     /pseudo
     CDS             complement(47..4663)
                     /locus_tag="JN692_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4756..5403)
                     /gene="rnt"
                     /locus_tag="JN692_001956"
     CDS             complement(4756..5403)
                     /gene="rnt"
                     /locus_tag="JN692_001956"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7264277.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5506..5913)
                     /gene="gloA"
                     /locus_tag="JN692_001957"
     CDS             complement(5506..5913)
                     /gene="gloA"
                     /locus_tag="JN692_001957"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7264278.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5994..7079)
                     /gene="nemA"
                     /locus_tag="JN692_001958"
     CDS             complement(5994..7079)
                     /gene="nemA"
                     /locus_tag="JN692_001958"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7264279.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISDSAQTKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQLWHTG
                     RISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGIVNDF
                     RQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGDSVENRARLVLEVVDAG
                     IEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGE
                     PYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQ
                     LKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7116..7715)
                     /locus_tag="JN692_001959"
     CDS             complement(7116..7715)
                     /locus_tag="JN692_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7264280.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSSEPLENALAHVKNIIATPAV"
     gene            7818..8057
                     /locus_tag="JN692_001960"
     CDS             7818..8057
                     /locus_tag="JN692_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7264281.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8106..9002
                     /locus_tag="JN692_001961"
     CDS             8106..9002
                     /locus_tag="JN692_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7264282.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9083..9604
                     /gene="sodC"
                     /locus_tag="JN692_001962"
     CDS             9083..9604
                     /gene="sodC"
                     /locus_tag="JN692_001962"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HAY7264283.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9605..11617)
                     /locus_tag="JN692_001963"
                     /pseudo
     CDS             complement(9605..11617)
                     /locus_tag="JN692_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11617..12474)
                     /locus_tag="JN692_001964"
     CDS             complement(11617..12474)
                     /locus_tag="JN692_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7264284.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNVAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12477..12713)
                     /locus_tag="JN692_001965"
     CDS             complement(12477..12713)
                     /locus_tag="JN692_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7264285.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            12914..13348
                     /gene="slyA"
                     /locus_tag="JN692_001966"
     CDS             12914..13348
                     /gene="slyA"
                     /locus_tag="JN692_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7264286.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13395..13862)
                     /gene="slyB"
                     /locus_tag="JN692_001967"
     CDS             complement(13395..13862)
                     /gene="slyB"
                     /locus_tag="JN692_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7264287.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14136..15245
                     /gene="anmK"
                     /locus_tag="JN692_001968"
     CDS             14136..15245
                     /gene="anmK"
                     /locus_tag="JN692_001968"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7264288.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15343..15672
                     /gene="mliC"
                     /locus_tag="JN692_001969"
     CDS             15343..15672
                     /gene="mliC"
                     /locus_tag="JN692_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7264289.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15731..16387
                     /gene="pdxH"
                     /locus_tag="JN692_001970"
     CDS             15731..16387
                     /gene="pdxH"
                     /locus_tag="JN692_001970"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7264290.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16516..17790
                     /gene="tyrS"
                     /locus_tag="JN692_001971"
     CDS             16516..17790
                     /gene="tyrS"
                     /locus_tag="JN692_001971"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7264291.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17852..18712
                     /gene="pdxY"
                     /locus_tag="JN692_001972"
     CDS             17852..18712
                     /gene="pdxY"
                     /locus_tag="JN692_001972"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7264292.1"
                     /translation="MKNILAIHSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(18756..19361)
                     /gene="gstA"
                     /locus_tag="JN692_001973"
     CDS             complement(18756..19361)
                     /gene="gstA"
                     /locus_tag="JN692_001973"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7264293.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19467..20969)
                     /gene="dtpA"
                     /locus_tag="JN692_001974"
     CDS             complement(19467..20969)
                     /gene="dtpA"
                     /locus_tag="JN692_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7264294.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21580..22215)
                     /gene="nth"
                     /locus_tag="JN692_001975"
     CDS             complement(21580..22215)
                     /gene="nth"
                     /locus_tag="JN692_001975"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7264295.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22215..22910)
                     /locus_tag="JN692_001976"
     CDS             complement(22215..22910)
                     /locus_tag="JN692_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7264296.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(22914..23534)
                     /gene="rsxG"
                     /locus_tag="JN692_001977"
     CDS             complement(22914..23534)
                     /gene="rsxG"
                     /locus_tag="JN692_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7264297.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23538..24596)
                     /gene="rsxD"
                     /locus_tag="JN692_001978"
     CDS             complement(23538..24596)
                     /gene="rsxD"
                     /locus_tag="JN692_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7264298.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         6126 a         6405 c         6448 g         5773 t
ORIGIN      
        1 tgaacgtagc agggaaccac gtccttcagg gcgtggagga tgtcaactat ccccaatcca
       61 gcccttgtgg cgaacttgaa aatcccgcct cacgcagcag cgtaaacatc ggcgtttgcc
      121 ggaccggaag atcattcact tctgttagcg taaagcgcag ccttggttcg cgacgcagtg
      181 cggtggtcag cgcctggaaa atctctgggg cgagtaattc ctccttttcc tgccagacca
      241 gcattttttt gccgccttgc gccagataga gtaacaattt gccgccagaa acgaccacca
      301 gcgcacccgc ccgacgcgtt ggtaccagcg ttgccggatg tgcaggccaa ggcagaagat
      361 ttccccacac attagccgga tcgttggcgg agagcgccac tggtgtatag tggcgtgttt
      421 gcgtggcttg tgtcgccaga tcgcgcaatc ggtcaatagt cagacgttca gcgaattgcg
      481 cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct tccatacttc
      541 gacaaagtgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc accgcctgac
      601 gactgatgat gccgtagcgg tcgagcatat tttccgccag cgctagcatc ctttcggtat
      661 cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt gtgttgtaag
      721 atacccgcgg cgagacgggt tgtgcataga caggacgccc acgacgagcc cggtgactgc
      781 ggcgagttga ggtgcgtgcg ttggaactgc tgcgggtgag ggcgcgtaac ggtgcccaaa
      841 tatcgctggt aatgacgcct tgccagacca gcgcccataa cgcctcttgc agtgccgagg
      901 aatcaaccga ttcgccgatt ttatcgcgta tccgttggct gatttgttgt gcaaaccagg
      961 ctcctccgtc agccagaaca gcgattatcg cttgctgcag cgccgaacga ttcgcctgat
     1021 ccgcttcggc gggagtgaac gattctgcgg catatttctg tagatgcagt gccaccaggc
     1081 cgtcatcttc acccagcttt ttttgtcccg accagataac cgctccggtt gccagtaatt
     1141 catcgagcat ttctggcgaa tagtcgcgta cgcgggcagg caggatctgg ctttcccaga
     1201 gtgaggcggg taaaccgact ccggcaagct gttcgatcac ccgcatcacg ccatctacgc
     1261 cctcataaac gcctggctat gttgaggcaa agagcgccgg gctaccatcg gtggcgggta
     1321 atacgccctg acgttccaac agcaatcgcg cataggtcgt ggctgcaacg ggacgcgtcg
     1381 cttctctggc ggcttgcagc gagcgcaaac gcagacgacg aaatacttca tcgctcaccc
     1441 agatgtcttg ttgcagattc atcaccagac cctgttcacg cagttgctga agctgctctt
     1501 cggcaatggc aataccgaga ctaaactcat gagccagttg ttcagccgtg accagagcat
     1561 gagcccggag atagcgcaga aagaggtcgc gaagcgggta actgactcta tgtaaataaa
     1621 tctctggcaa tgactctggt agtcgtacgc cgagggcatc acgcagcctg gcggcatcat
     1681 ccatacaggc aagacgctcc tgtccgctaa tcatcgctgg gaagattcgt tttactgcaa
     1741 gaagattttc cagatacgac gcaacctctt cactgctgcc tgtatgccgt tgcgccaggt
     1801 cttcaacggt cattggcccc agttcgcgca gcaggtcgaa caatccttct tcacctttcg
     1861 ctcttctgcc aggagccagt cgttgcaact cttcttccac ctggcgaatg acctgcgggt
     1921 cgagtaattc ccccggatcg acctgtccga gtagattgcg cagtaactcg ctgtccagcg
     1981 acagtacgga tgcccggcgc tctgccagcg gggcgtcgct ctggtacata aattccgcga
     2041 catagccgaa caataaactt gtggcgaaag gcgagggcgt agtggtcgtt acatcggata
     2101 tttgaatttc gccaccgttc agacgacgca tcaaacgttc cagtgcggga agatcataaa
     2161 catcttgcag acattcgcgt agggtttcga gaatgaccgg aaaatccgga tatccctgag
     2221 cgatttccag caactgactg gcgcgcagcc gttgttgcca aagcggggtg cgatggcccg
     2281 gcgtgcggcc cggcattaat aatgcccgcg cggcgcattc gcgaaaacgg gcggcgaaaa
     2341 gtgccgagct gcctaccgcc tcgcggacaa tttgcagcaa cttttctggt tcaaacaaaa
     2401 aaatcgcggc gtcgggcaat ttaccatcag tgtcaggaat gcgcgcaaca atgccgtcat
     2461 cactggcgac cacagacgcg tcagcgcccc atagggcatg tattcgcccg gcaatcgcca
     2521 gcgcccaggg ttcatgcacc cgtcttccat agggagagtg caaaataata cgccagtcgc
     2581 caatttcatc acggcaccgt tcgaggacca gatggcgact ccccggaacg atgcccgtcg
     2641 cgttgcgttg ctcgtcaatc aacccctgaa tattggcgtt cgtattttct tctgccagcc
     2701 acgggggaat agtcccggaa aagaacgcgc catccgccag taaatgaaga aaatcgccga
     2761 tcatctcgcc taattcagcc ggacgtccgt taccttcacc acgccagaag gggagccggg
     2821 cagaacgacc cggagcagga gtcacaatca cctgatcgcg ggtgatttgc tggatccgcc
     2881 atgaggtagc gccgagagtg ataatgtcgt tcacccgcga ctcatatacc atctcctcat
     2941 ccagttcacc cacccgccgc gaaccggcct tttcttcacc ttcgggtaat aacacgctat
     3001 acatgccacg atccggaatg gtgccgccgc tggtaaccgc caacaattga gcgccaggtc
     3061 gggcggtcaa tatcccggtc tccctgttcc agaccagttt ggggcgaaaa gcagaaaaat
     3121 cgccagaggg atagcgcccg gaaagcatat ccagcgtggc gtcaaaaaca cgtcttggca
     3181 gatctttcca cggtgcggca cggcgtacgc gggagtacca ttcgtctacc tgtaatgcat
     3241 ccatcgccgc ggcggcaacg gtttgctgcg caaggacgtc gagaggatta tgcggtggtg
     3301 tcaggttttc cagcctgcct gcgaacatac actctacaat gactgcggaa tcgactaaat
     3361 cacgccgggt acgggggaaa aacagccctt tagatacacc gccaacctga tgtccggcgc
     3421 gaccaatgcg ttgtaaccca ctggcaacag aaagcggcgt tgccacctga atcaccagat
     3481 ccaccgcgcc catatcaatc cccagttcaa gactggaggt tgcgaccacg cagcgtaatt
     3541 cacccgattt cagcgcctgt tcggtgattg ctcgttgttc tttagagacg gagccgtggt
     3601 gtgagcgggc aataaaaacg tcgctacttt gtacacggtt agaggttgcg ccggaggtcg
     3661 actcgaaatg ggccacatca acggcgatag acggggaacg ctgtaagcgt gcggcgtaaa
     3721 gctcatttaa tcgtgccgtc agtttttccg ccagcccgcg tgaattagta aagacaatgg
     3781 tcgagcgatg gcgcaacact tcatcaagga tacccgtttc aatatatggc cagatggagc
     3841 cttcccggcc ggcatggctg tcttcgccgg tgccgctggc gaccgatgag acatcatcca
     3901 tattggcgac cggtacgaca attcgtatct gcggatggcg cattgcgggc gggttgacta
     3961 ccgtaaccgg gcgatcgcca ccaagaaatg ctgccacatc gctggctgag cgcacagtgg
     4021 cagaaaggcc aattcgctgt gctgaggtgt ggagcagcgc atcgagccgc tccagactta
     4081 acgccagatg cgcaccacgt ttactgcccg ctaccgcgtg gacttcatca ataattaccg
     4141 tttcgacgcc gcgtagcgtt tcgcgcgcgc gggaggtcag catcagatag agtgattcgg
     4201 gtgtggtgat cagaatatcc ggcggattac gggtgagttt gctgcgttcc tgtgcaggcg
     4261 tatcgccagt acggatccct acgcgaagat tgacttccgt ttcgccgcgc cgccgccgtt
     4321 catcggcaat acccttcaac gggatctgca agttgcgctg aacgtcggtg cccagggctt
     4381 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4441 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4501 agccagtcgg tgcaatcacc agcgcatgtt cgcctcgcgc cgccacatgc caggtttgcg
     4561 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4621 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4681 aatatcatgc ttgatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     4741 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     4801 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     4861 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     4921 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     4981 gttacgtttc agcgaggcgc gttctgcggc ggccatcata aagctgtgat caaaattggc
     5041 gttgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     5101 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ccgcgatcgg gatcgttcgg
     5161 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     5221 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     5281 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5341 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5401 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5461 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5521 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccacaaa
     5581 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5641 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     5701 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     5761 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     5821 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     5881 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     5941 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     6001 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     6061 ttaagctcag ctttgagctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     6121 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     6181 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     6241 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6301 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatct
     6361 gtgttctgga aagtaccgat tggcgaaacg cgaatgccaa tgcgatcggc accccattct
     6421 tcaatcccgg catcgactac ttccagtacc aaacgtgcgc gattttccac gctgtcgccg
     6481 tactgatcgg tacgatggtt tgaagaaggg gaaaggaact gatgcagcaa ataaccgtga
     6541 gcagagtgga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6601 aaatcattga cgatacccgg aatctcttcc agttcaagcg cacgcggcat ggatgtttca
     6661 acgcggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     6721 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     6781 tgccacagct gcaaggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     6841 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttgtctgg
     6901 gcagaatcac taataatcaa accggcactg gcacgttggc gatagtattc cgccatcaac
     6961 ggggtaggaa tgtcacccgg ttcaatactg cgcagacgcg tcagcggtgc cataaaaata
     7021 cggtttgccg ccgtgatcgc gcccactttc agtggggaat acagtttttc agatgacata
     7081 aaggctcccg gtcgtctggt gataaaaaat gcctgctaaa cggcaggcgt cgcaataatg
     7141 ttttttacat gggccagcgc gttttccagt ggctcggaac tgcgcgaaat tttggcctgc
     7201 agattcgcgc caagccatag tgcgtaaagc acttgtgcct gttgtagcgg ttcgccacaa
     7261 aaggttaaac aatggttctc acggccattt tccagagcct gcgagagcag ggcgatcacg
     7321 ccgcgagcac ctttatccat cgcgctgcgc atatcttctg acagatcgca cacttcggca
     7381 gagagtttta ctgtcaggca accactgatg gttccatgtt ggcaaaactg gttcagtgtt
     7441 tgctggtaat aagccagtat gcggtcgcgg tagttacctt cgccggattg cagcaactca
     7501 gtcagtcgct ggtgatatgc ggcgtaatga cgctcaagca tggcaacgcc aaacgcttct
     7561 ttagagcgaa agtagtgata gaaggaccct ttcggcactt cagcggtttt tagtaattcg
     7621 cttagcccca tcccggtgaa tccacgttgc aggcaaagtt gctcgcccgt cgccaagaga
     7681 tgttcgcgag tatcatgttc ggtgtgtttg ttcatgcgtt ggagtgtagt agaccagtcg
     7741 gtctagtgca agcaaagttg ctactttgga cattaacagc ttcaataatg gtaataatct
     7801 tatctgagga gggcgaggtg gcggagcaat tagagttctt tcccgtccag agcccgtgcc
     7861 ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg ctgttttcgt agtcgggatg
     7921 agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca agaggtgctg cgcctttgtc
     7981 ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc gtcatcagac gaaccggagc
     8041 aaccatcact cttttgaacc taatcttgcg tatactcctc tcatatctga taagaggaag
     8101 cgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc cgttttgtga
     8161 tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc agttttattg
     8221 aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat ggtggctatc
     8281 agtgcgaagc ggcgtttggc gaggcactga aactggcacc tcacctgcgt gaacggatgg
     8341 aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt
     8401 acatcactga ccgcaatcac atcattaaga gcgccgaaca gtcgctaatt aatctcgcga
     8461 ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat gccgatgaag
     8521 tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt ggcgtatcga
     8581 actttacgcc tgcgcaattt gccctgttgc aatcacgtct gccgtttacc cttgccacta
     8641 atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggcacg ctcgaccaac
     8701 tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta
     8761 atgatgatta tttccagccg ctgcgtgatg aactggctgt ggtggcggag gagttaaacg
     8821 cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccgtcg cagccgctgc
     8881 caattatcgg ctcaggtaaa attgagcgcg tacgggcagc tgtcgaagca gaaacactga
     8941 aaatgacccg tcaacaatgg tttcgtatcc gtaaagctgc actggggtat gacgtaccgt
     9001 aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac ttattcagga
     9061 atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gccattctgg cgctggttgt
     9121 tgcgaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca cgtcgcaagg
     9181 ggttgggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc tggagttttc
     9241 acccgatctg aaagcattac cccccggtga acatggcttc catattcatg ccaaaggaag
     9301 ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag gcgggcatct
     9361 tgatccacaa aataccggta aacatgaagg gccagaaggt accgggcatt taggcgatct
     9421 gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg cgcctcgtct
     9481 gaaatcactg gatgaaatca aagacaaggc gctgatggtc cacgttggcg gcgataatat
     9541 gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg gtgtaattaa
     9601 gtaattacga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa agcgagcagt
     9661 acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga cgggcaaggc
     9721 tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc tgaacgccac
     9781 gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgc gataacggat
     9841 cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc agcagcacta
     9901 caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc aactgactga
     9961 tgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg cggcttagct
    10021 gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg gcttttacga gcatccgatc
    10081 ctggacgcag aatggcgaac gctagccaag ccagcgcaac gccaacgatt tttgccagat
    10141 tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg ataaacgaac
    10201 ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc aatttaagaa
    10261 gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga tcgctaatct
    10321 ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc agcgtgcgca
    10381 tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg cagctaattg
    10441 ctgccagcgt taatgcattg gcaccggcat cccattgcga tgcaatactc catgcgccaa
    10501 tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat agcttcggcg ttgtcggtat
    10561 gacgagccag agcggatgtc cgtgcgagtc tggcatgatc atctacaccg ctttgcagac
    10621 gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt cgctgccaga
    10681 aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata atacgtgcga
    10741 cggtgtaagc atctgtttgc gaactggcga gcgccgtcag caattgttcg agaatttctc
    10801 gcgtggcact tggcggtgag ggccagttga gcaacatacg tcgcaggctg gagatgacac
    10861 tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc tggcgaaaac
    10921 gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc tgcccaatca
    10981 ccccctcatg tgctgcacga atggcatcgt ttgtttcagg ctgccagagt aaactggcat
    11041 gttccagtaa tcgggcgtgc atgtttttca atgcagttaa aagggcagta gcatcggaac
    11101 tgctaggcag gatcatcatc atcatgccgc cgcacaaaat accgactatc acctcgcaaa
    11161 cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg
    11221 cgatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt
    11281 gcgcacaggc ctaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg
    11341 gctcattgag cgtatgtccg gcaagaagca gtgccgcaat ggctcccagt aaactgccag
    11401 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accactgcag
    11461 ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca
    11521 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccctgaacc
    11581 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
    11641 ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa agcggacggg
    11701 aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga
    11761 gtcactttca acgctctgat cataaatcgc ccgaccgata ctggaaacgt gaccctgtaa
    11821 cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa
    11881 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag
    11941 cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccatccact
    12001 caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtcgcctg
    12061 tgcgacatta acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
    12121 ggcaaaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
    12181 gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg
    12241 cgttttatcg atcgtgagca gaagttcccc cgcgttaacc agttggttgt cttttatatt
    12301 cagctcaaca atacgtcccg acacctgtgg ggtgatagaa acctgttctg cgcgtatttt
    12361 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
    12421 cgcaactatc gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac
    12481 cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt
    12541 aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca
    12601 agccagatga cgaatccaaa cgcgaatgct ttgaaaaacg gaggaaaata gacggacgca
    12661 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
    12721 attcctttac gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat
    12781 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
    12841 tagcaataca attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
    12901 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
    12961 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
    13021 gttacacaat atccatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
    13081 tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat
    13141 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
    13201 agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaatattaca
    13261 tggcatctcc gcagaggaac tggagcaact gattacgctc atcgcaaaac ttgagcataa
    13321 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
    13381 acacgcaagc ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg
    13441 acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta
    13501 ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg
    13561 ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt
    13621 ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat tgcaccgata
    13681 acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat ggtgccatag
    13741 ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg
    13801 ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc
    13861 attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat
    13921 atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ctaatgaaag
    13981 taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt
    14041 tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag
    14101 tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
    14161 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaatt gatgaacacc
    14221 gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa caggccgtac
    14281 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
    14341 tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga
    14401 gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg
    14461 caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg
    14521 ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgttggtac
    14581 ctgcgttcca tcatgccttg ctggctcacc caaccgagcg acgaatggtg ctcaatattg
    14641 gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg
    14701 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggcaggtaaa ccttacgata
    14761 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
    14821 gcgacccgta tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg
    14881 gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca
    14941 cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
    15001 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
    15061 cattactgct aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
    15121 tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta ccaggaaatc
    15181 tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc cccgccaact
    15241 cgtgatatca gaattaactg aattttttct tatcgtgtct gccgttacac ttggaagatt
    15301 aaggagggta tttgccctcc ggactaggaa agtcttcggg atatgactat gaaaaaactg
    15361 ttaattatca ttttgccggt ccttctctcc ggttgtagcg cctttaatca actggttgag
    15421 cgaatgcaaa ccgatacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg
    15481 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
    15541 ggcctttcag cctctggcgc gcgttactcc gacgggatct atgttttctg gtcgaaaggt
    15601 gaggaagcga ctgtctataa acgcgaccgc atcgtcttga ataactgtca gttacaaaat
    15661 ccacagcgtt gagatttttc caagggcggc gcacaatagc gtcacccact gacaatccgt
    15721 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
    15781 caccaaaggc gggttacgcc gccgcgatct tcccgccgat ccattaaccc tttttgaacg
    15841 ttggctctct caggcttgtg aagccaaact ggcggaccct accgcgatgg tggtcgctac
    15901 cgtggatgaa catggtcagc cttatcagcg catcgtttta ctcaaacatt acgacgaaaa
    15961 aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc
    16021 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
    16081 agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
    16141 ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
    16201 aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccgagctt
    16261 ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
    16321 cctgcatgac cgctttttgt accagcgtga aaatgatgcg tggaagattg atcgtcttgc
    16381 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
    16441 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
    16501 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
    16561 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
    16621 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
    16681 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
    16741 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
    16801 actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac
    16861 tgcggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
    16921 ctgaccttcc tgcgcgatat aggcaaacac ttctccgtta accagatgat caacaaagaa
    16981 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
    17041 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacgatgt ggtgctgcaa
    17101 attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
    17161 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ccaaagcaga cggcaccaaa
    17221 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
    17281 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
    17341 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
    17401 gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa
    17461 ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg
    17521 agtgaagcgg acttcgaaca gttggcgcag gacggcgtac cgatggttga gatggaaaag
    17581 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
    17641 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
    17701 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
    17761 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
    17821 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccactctca
    17881 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt ccgatgcgcc gcctgggcgc
    17941 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac
    18001 tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
    18061 taaattacac acctgtgatg ccgtcttaag tggctatctg ggatcggcgg agcagggtga
    18121 acatatcctc ggtatcgtcc gtcaggtgaa agcagcgaat ccgcaggcga aatatttttg
    18181 cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    18241 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg ttgagctgga
    18301 aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    18361 cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg gctacagccg
    18421 tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca gccgtccact
    18481 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    18541 ggtgaaactg cttcaggggg caacgctgca ggaggccctg gaacatgtga ccgctgcagt
    18601 ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag tggtggctgc
    18661 tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct gaaatatctc
    18721 atttcggccc acaacgacct gtgggccgat ggctattact ttaagccttc cgctgacagc
    18781 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgccgcaat gtgctctaac
    18841 ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag ataggcatca
    18901 gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgcctc gttcacatat
    18961 tgcagcttct tatccagctg cgcgcgaact gtcggtttgt actcttccgg tgtatctggg
    19021 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    19081 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    19141 gcaagatact gcataatcgc aacgccttcc gtcagcaaag taccatcgtc cagcagcaat
    19201 gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    19261 ttcattaaat ccacactgac gagcgtaaaa tcctttccgc tctcacgcag ggtgatatgg
    19321 gaagcgagag agcaggcgcc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    19381 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    19441 ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc tttgtctgca
    19501 gcgtcatcct gcgtcatgcg gtgcagttta ggcgcggtca gcagcatcag tactgcaata
    19561 acggcagtag cgacaccaat ctgcaagaat acgcgaccat agacttccag tgacatcagc
    19621 gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt
    19681 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    19741 agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat gctctgcagg
    19801 ccatagcttg cgaccagcca gctgacagac acgatacctg cgtcagacgc gaatttcgca
    19861 cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc aaacttggtt
    19921 ggcatcggca gggtatcacc catcttgtta tagatagcgg ccagaatcgg actaccgatg
    19981 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag acccagaatg
    20041 gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc
    20101 acgaagaaga tgattgcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    20161 gcacctttca tcgcgaaagc ttctttaccg aagataacca caataccgaa ggcaacaacg
    20221 cccagcgcca tacgcgcaac ttcctgattg tgcagtagcc aggtggcaac agcgatcagc
    20281 gccacaacac caataatggt cagcaacagg ttacggtagt tgactggttc aaagtctggt
    20341 tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc
    20401 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggg
    20461 gtggctatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    20521 ccgtccagac gtgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    20581 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacaat accggcgtcg
    20641 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    20701 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    20761 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    20821 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    20881 tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt
    20941 tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac
    21001 gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata gtgggagggg
    21061 aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt
    21121 acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt
    21181 tcaaaaaacg taacggcaga ttttttcact gcattgagag ggggatgatg gcttgacggc
    21241 gatatgttct gctatttagc gtttttccta gtgagatagt ccagtttctg aaaaatagcc
    21301 agtgtaatgt tttgcaggtc aatatttgtt gcgtgtgtaa atttaaatca ttttaacctg
    21361 attcttgcaa tgtttttaca ttgccacagc atcgtgatct tgatcacgaa tgaatagaaa
    21421 atttcgctat taaaaaaaca attaacctgc atttttggtt atccgcaaac ggattacctg
    21481 atgataacgc taagcaatgg catcattgac gttaaaaaga tgttttaaaa gaggataaag
    21541 aaaggttatc aatggggtaa tcggtgttac cccttttctt cagatgtcaa ctttctcttt
    21601 gtattcacaa agatcttcaa taatacaaga gccacagcgg ggcttgcggg caatgcaggt
    21661 ataacgcccg tgcaggatca accaatggtg gcagtcgact ttaaactctg ccggaaccac
    21721 tttcagtagc ttttcttcta cctgttcgac gtttttcccc ggcgcaaatt gagtacgatt
    21781 acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc cagccgaatg cagtgtttaa
    21841 tacgacgttg gctgttttac gacctacgcc gggcagggct tcaagcgcag cacgatcttc
    21901 cggaacctcg ccattatgtt gttccagtaa tatacggcag gttttgatga tattttctgc
    21961 tttgctgtta taaaggccaa tcgttttgat ataggttttc accccttcaa cgcccagttc
    22021 aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc gccgtcgcct tattaacact
    22081 gacatcggtc gcctgagcgg aaagcagtac ggcaatcagc aattcaaaag gcgaactgaa
    22141 attaagctcg gtggtgggat gaggattgtt ctcacgcagg cgagtgagga tctccagacg
    22201 ttttgctttg ttcatcagac attccctgtt tcaccgtttg gcaatgcacg ttcggcagct
    22261 gcttcagcac ggcgcttttt cattctttca tcaatcaggt attttcctgc cagcatcagt
    22321 cccaagccaa taaatgcacc tggtggtagc atcgccagca ggaaagggga gtcggtgtgg
    22381 aaaatctcca cgcgtaatac ttttgcccag ctacctaaca gcgcatctgc accgtcaaac
    22441 aacgtgccat tgccgataat ttcgcgtagt gaacccagca cgaacatggc gcaggttgcg
    22501 cccgtaccaa ttgaaaagcc gtccagtgcc gaaagcgccg gacctttttt ggcggcgaag
    22561 gcttcagcgc ggcccacaac gatacagtta gtgacaatca gcggaataaa aatccctaat
    22621 gattgataca ggccaaaggc gtaggcgttg atcagcatct gtacagcgct gaccaccgag
    22681 gcgatgatca tcacgtaaat gggaatgcgg atctcggctg gcgtccagtg acgcagcgtc
    22741 gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg caagtcctaa acccagagcg
    22801 ttagtggcag tggacgtgac cgccaatagc ggacaaaggc cgagcagttg gaccagtgca
    22861 gagttgtttt tccacaaccc ctgaacaata acgtctttaa tttcgctcac gctttattct
    22921 ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct gagcgtacaa tccggcgcgt
    22981 tttaccgcat taaccaccgc gcggggagta atcgtcgcgc cggtgaactg gtcgaaatca
    23041 ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac cactgatttt ttttcccgca
    23101 aaatgggtga tccagtcaga aaggcgcaat tcgattttat cgccaagccc tggcgtttcg
    23161 tggtgctctg tcacgcgcgt gccaagtacc gtgccattaa aatcggctcc caccagcagc
    23221 tgaatcgcac cggaatagcc atctggcgcg gttgcttcca gaacggcggc taccggtttg
    23281 tcatcctgtt tggcgatgta aacccgatgc tcacctttac ctaactctgg cgcagttacc
    23341 agatagcaac tctgtgcgag cgcattgtta tagcgttcgg ctggcagcac ctgatcaaat
    23401 aacgcctttt gttgcagact ggcctgttca gcaattgtcg ttttggtcat ctggttgatg
    23461 gccgcagtta accctgtaga acccgctgca aatagtgcca gtgtaatgcc gtgttttcgg
    23521 atagttttca gcatggttta ccctttgcga tggccgtaga cgcgcggacg cgtgtagtaa
    23581 tcgatcagag gaaccgtgat gttcgccagc aggacggcaa aagccacgcc gtcaggatag
    23641 ccgccgaaac tgcggatcaa ccagactaat aagcccgcca gcgcgccgaa catcagccga
    23701 ccacgattgg tcgtagaagc agtaactggg tcagtcaaaa taaagaatgc gccgagcata
    23761 gtcgcaccag acagcagatg aatttgcggt gctgccagtg tttctggtga gaacaaccag
    23821 cccaacgttg cgcataacgc cagcgttact aagaagctga ggggaatatg ccagcgaatc
    23881 gctttctgcc atagcaacca cacgccacca gccagccagg cgagatttac ccattgccaa
    23941 ccagcgcccg ccagaatacc gctgtagatc ggatattgca taatctgttc aaccgaatga
    24001 cctgcgcgga cagaggtttt gaatgtatcc agcggtgtcg cctgactaat gccatcaata
    24061 cctaagcgta gcgtattcat atcaccacca ctggcggtat gaccgctgaa aataacctgt
    24121 atggcgtcga taaaaccagg gatgttgacc gcaatttcat gtggcggtaa ccagctggtc
    24181 atctgcaccg ggaaggagat cagtaagacc acataaccaa tcattgccgg attaaacggg
    24241 ttttgcccca gaccgccata caactgttta gcgatgatca ccgcaaacac cgtacccagt
    24301 acgaccatcc accatggcgc taggggggga atacttaccg ccagcaataa gcctgtcagc
    24361 aatgctgagt tatctttcaa cgttgcggct accgactgct tgcgtagttt gagtacgaga
    24421 gcttcggcta acagagcact gaccgacgcc aacaggatct gaacgagagt accccaacca
    24481 aaaaaccaca gttgcgctgc gattcctggc acggctgcga gcaacaccaa cagcataatg
    24541 cgcgatgtct ggcgctggtt atgggtataa ggggagctag ctattctgaa taccatttag
    24601 tcctcgttta caaccttttg ctgggcggct tttttggcct gaacgcgggc aatagccgcg
    24661 gcaactgcgg ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt
    24721 tccagcttgc gtgctttggc gcgggcaata gc
//
LOCUS       DADLSE010000049        24589 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLSE010000049 DADLSE010000000
VERSION     DADLSE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.049"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN692_001979"
     CDS             complement(58..1083)
                     /gene="tdh"
                     /locus_tag="JN692_001979"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7264299.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN692_001980"
     CDS             complement(1093..2289)
                     /gene="kbl"
                     /locus_tag="JN692_001980"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7264300.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN692_001981"
     CDS             complement(2564..3421)
                     /gene="yibB"
                     /locus_tag="JN692_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7264301.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3725..4657
                     /gene="rfaD"
                     /locus_tag="JN692_001982"
     CDS             3725..4657
                     /gene="rfaD"
                     /locus_tag="JN692_001982"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7264302.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4667..5713
                     /gene="rfaF"
                     /locus_tag="JN692_001983"
     CDS             4667..5713
                     /gene="rfaF"
                     /locus_tag="JN692_001983"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7264303.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5717..6709
                     /gene="rfaC"
                     /locus_tag="JN692_001984"
     CDS             5717..6709
                     /gene="rfaC"
                     /locus_tag="JN692_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7264304.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGVAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPRLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6706..7914
                     /gene="rfaL"
                     /locus_tag="JN692_001985"
     CDS             6706..7914
                     /gene="rfaL"
                     /locus_tag="JN692_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7264305.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7950..9092)
                     /locus_tag="JN692_001986"
     CDS             complement(7950..9092)
                     /locus_tag="JN692_001986"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7264306.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9101..10114)
                     /locus_tag="JN692_001987"
     CDS             complement(9101..10114)
                     /locus_tag="JN692_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAY7264307.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKELPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDEAIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN692_001988"
     CDS             complement(10139..10846)
                     /gene="rfaY"
                     /locus_tag="JN692_001988"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7264308.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAAKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN692_001989"
                     /gene_synonym="rfaI"
     CDS             complement(10872..11879)
                     /gene="waaO"
                     /locus_tag="JN692_001989"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7264309.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNLRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN692_001990"
     CDS             complement(11922..12719)
                     /gene="rfaP"
                     /locus_tag="JN692_001990"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7264310.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12712..13836)
                     /locus_tag="JN692_001991"
     CDS             complement(12712..13836)
                     /locus_tag="JN692_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7264311.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQSKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN692_001992"
     CDS             complement(13833..14891)
                     /gene="rfaQ"
                     /locus_tag="JN692_001992"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7264312.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYLDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQDGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            15304..16581
                     /gene="waaA"
                     /locus_tag="JN692_001993"
     CDS             15304..16581
                     /gene="waaA"
                     /locus_tag="JN692_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7264313.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            16589..17068
                     /gene="coaD"
                     /locus_tag="JN692_001994"
     CDS             16589..17068
                     /gene="coaD"
                     /locus_tag="JN692_001994"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7264314.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN692_001995"
     CDS             complement(17107..17916)
                     /gene="mutM"
                     /locus_tag="JN692_001995"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7264315.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLLLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN692_001996"
     CDS             complement(18014..18181)
                     /gene="rpmG"
                     /locus_tag="JN692_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7264316.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN692_001997"
     CDS             complement(18202..18438)
                     /gene="rpmB"
                     /locus_tag="JN692_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7264317.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(18655..19323)
                     /locus_tag="JN692_001998"
     CDS             complement(18655..19323)
                     /locus_tag="JN692_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAY7264318.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            19495..20715
                     /gene="coaBC"
                     /locus_tag="JN692_001999"
     CDS             19495..20715
                     /gene="coaBC"
                     /locus_tag="JN692_001999"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7264319.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            20693..21151
                     /gene="dut"
                     /locus_tag="JN692_002000"
     CDS             20693..21151
                     /gene="dut"
                     /locus_tag="JN692_002000"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7264320.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     QGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            21258..21854
                     /gene="slmA"
                     /locus_tag="JN692_002001"
     CDS             21258..21854
                     /gene="slmA"
                     /locus_tag="JN692_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7264321.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN692_002002"
     CDS             complement(21891..22532)
                     /gene="pyrE"
                     /locus_tag="JN692_002002"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7264322.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN692_002003"
     CDS             complement(22598..23314)
                     /gene="rph"
                     /locus_tag="JN692_002003"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7264323.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            23441..24304
                     /locus_tag="JN692_002004"
     CDS             23441..24304
                     /locus_tag="JN692_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7264324.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEIMQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
BASE COUNT         6556 a         5963 c         5461 g         6609 t
ORIGIN      
        1 acataattaa cagattatcc tcagataaaa gggctggaat accagccctt gttcgtgtta
       61 atcccagctc agaataactt tcccggactg gcccgaacgc atagcgtcaa agcccttctg
      121 gaaatcatcg atagagaaac gatgggtaat gatcggcgag agatcgaggc cagactgaat
      181 cagcgccgcc atcttgtacc aggtttcaaa catctcacga ccgtaaatgc ctttaatgaa
      241 caagccttta aagatcactt tggtccagtc gatagacata tcagacggtg gaatacccag
      301 catcgcaata cgaccgccgt ggttcatggt atcgagcatg gtacgaaacg ctggcggcgc
      361 gccggacatt tccagaccga catcaaagcc ttcggtcatg cccagttcag ccatcacatc
      421 attaaggttt tctttcgcga cgttaaccgc acgggtgata cccattttgc gcgccagctc
      481 aaggcggtat tcgttaacat cggtgatcac cacattgcgt gcaccaacgt gtttcgccac
      541 cgccgcagcc ataataccta tcggacctgc accagaaacc agcacatctt cgcccaccag
      601 atcgaacgac agcgcggtat gcacggcgtt accgaaggga tcaaaaatgg aagccaggtc
      661 gtcggagata ttgtcgggga ttttgaaagc gttgaacgcc ggaatcacca gatattcggc
      721 gaagcagccc ggacggttaa caccgacacc aatggtgttg cggcacagat gggtacgacc
      781 gccacgacag ttgcgacagt gaccgcaggt gatatggcct tcgccagaaa cgcgatcgcc
      841 gattttgaag cctttcactt cctgaccaat acctaccact tcaccgacat attcatggcc
      901 cacgaccatc ggcaccggga tggttttttg cgaccactca tcccagttat agatgtgaac
      961 gtcagtcccg cagatggctg ttttacggat tttaatcagc agatcgttat gcccgagttc
     1021 cggtacaggg acgtcggtca tccagatgcc ctcttccgct ttcagtttgg ataacgcttt
     1081 catctcacat cctcaggcga taacgcccag ttgtttacca atacgcgtga atgcttctac
     1141 tgcacgcgta atttgctcag gggtatgcgc cgcagacatc tgggtacgaa tacgcgcctg
     1201 acctttcgga acgaccggat agaagaaacc ggtgacgtaa atgccctctt tttgcagctc
     1261 tcgggcaaat ttctgcgcca ctaccgcatc accaagcatg accggaataa tggcgtgatc
     1321 ggctcccgcc agggtaaagc ccgccgccga catttgctca cggaactgac gcgcgttcgc
     1381 ccacagacgg tcacgcaact cgctacccgc ttcaaccatc tccagcactt tgatggaagc
     1441 ggcgacaatc gctggtgcca gcgagttgga gaacaggtac ggacgagaac gctggcgcag
     1501 ccactcaacc acttctttgc gcgccgcggt ataaccacca gaagccccgc ccagcgcttt
     1561 accgagcgtg ccggtgatga tgtcgacacg ccccatcaca tcgcagtatt catgggaacc
     1621 acgaccattt tcaccgacaa aaccgaccgc gtgggagtcg tctaccatca ccagggcatc
     1681 gtatttatct gccagatcgc aaacgccctt cagattggca atcacgccgt ccatcgagaa
     1741 cacaccatcg gtggcaatca gcacatgacg cgcaccggct tcacgcgctt ctttcagacg
     1801 agcttcaagc tcctgcatat cgttgttggc atagcgatag cgtttagctt tacacagacg
     1861 cacaccatca ataatagacg cgtggttcag tgcgtcggag ataatggcgt cttccgcgcc
     1921 cagcagcgtt tcaaacaggc caccgttagc atcaaagcag gaagagtaga gaatcgcatc
     1981 ttccatcccc aggaatgccg ccagtttttg ttcaagctct ttatggctgt cctgagtgcc
     2041 gcaaataaaa cgcaccgaag ccatgccgaa accgtgagaa tccattcccg cctttgctgc
     2101 cgcaatcaga tccggatgat tcgccagccc gagatagttg ttggcacaaa agttaatgac
     2161 gtggcttcca tcagccacgg tgatatctgc ttgctgcgca gacgtaataa tgcgctcttc
     2221 tttaaacaac ccttccgccc gtgcggtttc cagatcgttg gttaactgct gataaaattc
     2281 tccacgcatt gcgattctcc agacagggca aattccagca catattaccc aaacttatag
     2341 gtcgcgacga gatgatgcgt taacacttct gcaaaattca ggataaataa cgtgtatctt
     2401 gatgtctctc aacacctaca gatagtagaa aaactgtaca gttttggctg agcataatga
     2461 tgagtgattt tactgcattg atataaaaag aaaaaatgat gccaaagcat catttttcct
     2521 atctcagttg gcatcaaaac gctacccact aatggaggga aacctatttt cttgaaagga
     2581 aaattctcag cgcctgcatt tttgccccta aagtgttact cctgaagcag cgaaacaaat
     2641 cgaaccattt tcctctcccc agatagttta ggttgaagag atcggaacgt ttgtaatagc
     2701 acatgacaaa aatgccctgg tcatcgtcca caatgttatt gtttagtgtt attttttgac
     2761 tttccagaac taatttatag aattctttcc atttatgctg tgaaccaaca attgcgcccc
     2821 caataatata tacatgattg ccaatcatga aatcaaaagc ctgttgttga gatgtaacag
     2881 taagcccttt ttttatagtg aaaagatgca tcttattttc atcaaatggg aaatcccaaa
     2941 ttttcaatcc tcgagttaca tttggcttac ggcaatagcc aaaatctatc cacgcaacaa
     3001 gaggcgtttt cactaaaccc atgttgatag ctttatttac aaaataggcc ttaaggttac
     3061 ataccaaaac atactctggt gaccagtact ctgggttttt taattgtcga ggctcaagtc
     3121 tatttgtaaa cgactcatct ttttgaattt tttcgatacg acttctgata tatctaaatt
     3181 tcttttttat atcgataaca ataaccgttg ttggttttcc gttgcgaatc gcttcaatcc
     3241 ttggtttcaa atcgggagaa gtgaaaataa tcatctcatt ttcaagtgcg gccagacgct
     3301 caaagtaact aaaataaaca tcaactgaac gagctagttt ttcacggaac cctttattag
     3361 cagtccaatc ccctctacca atgtcaaaat atgcagtgat aattgtggtg gatgatttca
     3421 ttactaataa gaccattaat ataaaaaaag aattatatca taaatattaa cagaatgtat
     3481 actgtatatt atccattcaa atgattccgt tatttatcat acatcggaat attgatacta
     3541 aagcactatt cacatgcaaa accaacatcc gccatgaagg actagctaaa acccaaacta
     3601 gtttgttgca attagcatcc ttgcacctct atgtaaaggg ctgaagggat tcggatgtga
     3661 tggtatgatt acagacattc gtgtctgaga ttgtctctga ctccataatt cgaaggttac
     3721 agttatgatc atcgttaccg gcggcgcggg ctttatcggc agcaacatcg ttaaggcctt
     3781 gaatgataaa ggcatcaccg atattctggt ggtggacaac ctgaaagacg gcaccaagtt
     3841 tgtgaacctg gtggatctgg atatcgcgga ctatatggat aaggaagact tcctgatcca
     3901 gattatggct ggcgaagagt tcggtgatgt cgaagcgatt ttccacgaag gcgcatgctc
     3961 ttccaccacc gagtgggatg gcaagtatat gatggataac aactatcaat actccaaaga
     4021 gctgctgcac tactgtctgg agcgcgaaat cccgttcctg tatgcctctt ccgcagccac
     4081 ctacggcgga cgcacctccg actttattga atcccgcgag tacgaaaaac cgttgaatgt
     4141 ctacggttac tcaaaattcc tgtttgatga atatgttcgt caaatcctgc cagaagcgaa
     4201 ctcgcagatt gttggcttcc gctatttcaa cgtttatgga ccgcgtgaag gccataaagg
     4261 cagcatggcg agcgtcgctt tccatctcaa cacccagctt aataacggtg aatcaccgaa
     4321 gctgttcgaa ggtagcgaga acttcaaacg tgacttcgtc tatgtgggcg acgtggcaga
     4381 tgtaaacctg tggttcctgg aaaatggcgt ttccggtatc ttcaacctcg gcaccggtcg
     4441 tgcagaatcc ttccaggcag tagcagatgc tacgctggct tatcacaaga aaggccagat
     4501 cgaatacatt ccgttcccgg ataagctgaa aggccgctac caggcgttca ctcaggcaga
     4561 tctgacaaat ctgcgcgcgg cgggttacga caaaccgttc aaaaccgttg ctgaaggtgt
     4621 aacggaatac atggcctggc tgaatcgcga cgcataagag ctctgcatga aaatactggt
     4681 gatcggcccg tcttgggttg gcgacatgat gatgtcgcaa agtctctatc gcacgctcca
     4741 ggcgcgctat ccccaggcga taatcgatgt gatggcaccg gcatggtgcc gtccattatt
     4801 atcgcggatg ccggaagtta acgaagctat ccctatgcct ctcggtcacg gagcgctgga
     4861 aatcggcgaa cgccgcaaac tgggtcatag cctgcgtgaa aagcgctacg accgcgccta
     4921 cgtcttacca aactccttca aatctgcatt agtgcctttc ttcgcgggta ttcctcatcg
     4981 caccggctgg cgcggcgaga tgcgctacgg tttactcaac gatgtacgcg tgctcgataa
     5041 agaagcctgg ccgctaatgg tggaacgcta tgtcgcgctg gcctatgaca aaggcattat
     5101 gcgtaccgca caagatctgc cgcagccatt gttatggccg cagttgcagg tgagcgaagg
     5161 tgaaaaatca tatacctgta atcaattttc gctttcatca gaacgtccga tgattggctt
     5221 ttgcccgggt gcggagtttg gtccggcaaa acgctggcca cactaccact atgcggaact
     5281 ggcaaagcag ctgattgatg aaggttatca ggtggttctg tttggctctg cgaaagatca
     5341 tgaagcgggc aatgagattc ttgccgcttt gaataccgag cagcaggcat ggtgtcggaa
     5401 cctggcgggg gaaacacagc ttgatcaagc ggttatcctg attgcagcct gtaaagccat
     5461 tgtcactaac gattctggcc tgatgcacgt tgcggcggcg ctcaatcgtc cgctggttgc
     5521 cctgtatggt ccaagtagcc cggacttcac accgccgcta tcccataaag cgcgcgtgat
     5581 ccgtctgatt accggctatc acaaagtgcg taaaggtgac gctgcggagg gttatcacca
     5641 gagcttgatc gacattactc cccagcgcgt actggaagaa ctcaacgcgc tattgttaca
     5701 agaggaagcc tgacggatgc gggttttgat cgttaaaaca tcgtcgatgg gcgatgttct
     5761 ccatacgttg cccgcactca ctgatgccca gcaggcaatc ccagggatta agtttgactg
     5821 ggtggtggaa gaaggggtcg cacagattcc ttcctggcac gctgccgttg agcgagttat
     5881 tcctgtggca atacgtcgct ggcgtaaagc ctggttctcg gcccccataa aagctgaacg
     5941 caaagcgttt cgtgaagcgc tacaagcaga gaactatgac gcagttatcg acgctcaggg
     6001 gctggtaaaa agcgcggcac tggtgacgcg tctggcgcat ggcgtaaagc atggattgga
     6061 ctggcaaacc gctcgcgaac ctttagccag cctgttttac aattgtaagc atcatattgc
     6121 aaaacagcag cacgccgtag aacgcacccg cgaactgttt gccaaaagtt tgggctatag
     6181 caaaccgcaa acccagggcg attatgctat cgcacagcat tttctgacga acctgcctac
     6241 agatgctggc gaatatgccg tatttcttca tgcgacgacc cgtgatgata aacactggcc
     6301 ggaagaacac tggcgagaat tgattggttt actggctgat tcaggaatac ggattaaact
     6361 tccgtggggc gcgccgcatg aggaagaacg ggcgaaacga ctggcggaag gatttgctta
     6421 tgttgaagta ttgccgaaga tgagtctgga aggcgttgcc cgcgtgctgg ccggggctaa
     6481 atttgtagtg tcggtggata cggggttaag ccatttaacg gcggcactgg atagacccaa
     6541 tatcacggtt tatggaccaa ccgatccgag attaattggt gggtatggga agaatcaagt
     6601 agagtgtcgt tctacaagta tgtctcttgc agatttgcca gctcaaaccg tttttcaaaa
     6661 tttaaatctt gaaataataa ccaataagtt gacatcggag ataagatgac ctcaacatta
     6721 tttttctctc tcgagaaaaa aaactggata gcgtactgga acagagctct cgtattctta
     6781 ttcattacca cctatttttt gggtgggatt acaaggtata aacatcttat tgttattctt
     6841 atgacaataa cgacaatcgt ctatctctgc aaacggccaa aacactatct ctcactattt
     6901 aaaacatttc tttttggtag tgttgccata ttaactattg ctgcattgct gtcacttctt
     6961 caatcccctg atgcaggtgc tagcatgaag gaaattttca aagctattat tgagaatact
     7021 ttactatgca caatagcaat accggtcata ttgagagacg agaaaagaga agatgtcgaa
     7081 aaaatcgttt tcttctcatt tattagtgcg ttgggcttac gctgtttttc tgaattgatt
     7141 acctattata aggactatca acaagggata atgccattcg cagattatag acaccgtagc
     7201 atttctgact cgatggtctt tttattccct gcattgttaa atctctggct tatcaaatca
     7261 gcaaaatacc gcatttcttt tgtggttcta agcgttattt ttatttttct gatattagga
     7321 actttatcca gaggggcctg gctttccgtg ttagtcattg gattaatatg gattctgatg
     7381 tttaaacaat ggaagttact attagtagga gtaatggttt ccatcattgc attgtcggtt
     7441 attttcacac ataaggagat gaccgcaaag ctaacgtata aacttcaaca aactaatagt
     7501 tcttatcgct atgcaaatgg tactcaaggc agcgcactcg atctaatatt agaaaatcct
     7561 gttattggtt atggttacgg taacgttgca tataaagatg tctataataa acgtgtcatt
     7621 gattatccag aatggacctt tagacaatca atagggccac ataattttgc gctattcatc
     7681 tggtttggca ctggtttatt agggctggta agtcttatga tgctatactg tgcaatattg
     7741 aaagagtgta taaaaaatgg cgtcaagaat aaatatcgct caccatataa tgcatattat
     7801 ataatcttac tatcttttat aggttatttt gttatccgtg gaaacgtaga acaaattgaa
     7861 ccaaatttat taggcgttta cgccggctta ttattagcga tgaaaaacaa gtaaaaaata
     7921 aaaaaggctg cataatgcgg cctttttatt tattcaaacc aattatgaat aacctcttcg
     7981 aatctttgag ttacgccatc ccaactgtag tgatcaaaca caaagtcctg accttgcttt
     8041 gcaactgctg ttaattcagg attcgctaac gtttttaaaa tatcgctgct tatggagtct
     8101 gcagtcatag gttcttttaa atggaatccc gtagtatttt ctttgacaaa ttccgtcatc
     8161 cccccacgcg tactcactaa aacaggctta ccagcaccca tagcttcaat tgccaccatg
     8221 caaaatggtt cctggaactg ggaaggaatg acgactaaat ctgccagtgg gtaataacaa
     8281 tgcatctttt caggcggtat gcttcctaac ataatgcaac gatccttcaa acgttttgca
     8341 atttccctga cattgcgttg atatgcacct ttgtcacttt tactcatttc tgtataatca
     8401 ccaatcacaa ctaattttaa atttttgtga gcagcggcca atttttcgaa tgattgcatc
     8461 aacagcaata tgcctttgtc agggacgata cgcccagcat aaaaaataat tttctcttca
     8521 ggggagatat taagttcgct tctagtgatc ggctgaaaat tagattgata ggtttctaaa
     8581 tcaattccat ttggtacaat ctcaatatct gcatttgcta aataagattg gtaatacttt
     8641 ttcaagtata aactagggac tatcatttta acattttgct caagcaattt tggttcaaat
     8701 gcattatgca tatgtataac cactcttgcc tgaggagctc gcttacgaat ttgagtgtat
     8761 agcttcatgc tattatgaac tacaataaca ctttctttcg ttataggaaa atcatgtgca
     8821 atattcaata ttctctgtga atatggaaga ggatcaagtc ttgtccattt ttgaaataac
     8881 cgcttatata tgcgactgaa tccaacccta tgaatactac aattatcatt aacaaaggtg
     8941 taatttgagt atcctggatt tttgatacaa acaatacgat ttggaaaatt ggtacgctga
     9001 gcaacctgat acatccacgt ttcaacagca gccgctccgc gaggaggaat tgaaaaaata
     9061 ggagtaaccg taaatattat tttatcaacc atctcgtata ttaacctttc ataacattat
     9121 atttaattgc tgtaaaaaca ccatcgagaa attttttctg gtaaagcaag tgtttatatt
     9181 tttctttgtg ctcatgtttt ttaacagctt ttttataagg tatatttctc catggtgata
     9241 tattataaat attacgaaaa taatctgcag aggcgtagtt cgcccaatca tgccatggct
     9301 tagttatacc agtataatgt atgaagacag tgtcatcatt aataaaccgg gtgtaatatt
     9361 cactattttt ttcttcaaat tctgacttta tagtataaat acaattatat tttctcggta
     9421 atattttagc atgatgtaga aacattaagt ttaaaatatc ttgatccggg tactttaatt
     9481 tttttataat tgcctcatct gataatagat caaagaattt ttccgttagt ctttgtttta
     9541 accattcccg taaattaata tacattacac cagagttaaa atagctaccg ttaaaatcct
     9601 cattacacaa acgctcagcg cttttacttt gcatagcatc tacatcaatt accactgccc
     9661 catattcatc tttaaactct aatgctatta actcgttcaa tgaaccctta cagacgatat
     9721 cagcatcaag atataataat gaatccaatc gcgcagaaaa ataatcaaat gaaagcaaac
     9781 gataataaat agatactggc cagatatttg attgaggtag ctccttcaat ggctcagaat
     9841 caatatgata taatttaatt actgtttgat acttcgttgc taattgtgat aataattcaa
     9901 tatactcttt gtctaagtaa tcagcgatta tgtgaaaggt aaatttgttt tgtttattat
     9961 tttcaagaat tgaagcaatt gaaatcgcag ccccaacctg gtagttctca tcaatacccc
    10021 atgagacatt aaacgtttcc cgttcatcaa gtttaacggg gcgcttgtcc agttcaatta
    10081 tatctttaaa ttgagtaaga tgtttaaaat ccaatttaat tacccttcat cataatcatt
    10141 atttgctctt caatcctagt ttgactttta catccctgat tactttttta attttctttt
    10201 tgaatataag atatgtatag ccaaagtctt tcaattcatt ttttatatta tagtgccgtt
    10261 caagatcaat gcgatctttt gcttttaaaa cagcggttgt ttttttacct gaaaggtcaa
    10321 ttagtcttaa acctttttcg gatactataa aatttccttt atgaggatcg ccagacacca
    10381 ttccatgctg atgcaactct tttatcgatt ctgataattg atccttcaga tcttcagaaa
    10441 tttccaaata ctcatttaac ccgacacctt caatatactc aatgagcata atataataat
    10501 gagcgaaatt taatgtttta cgttcagcta gcaaaaaata gtcattaatt gattggatgc
    10561 cttcaccacg tacccgatcg gtttgataaa ttaaattttc ataataatct ttttttatgc
    10621 aagattttaa gaaacgttcg gtcattttat gctttgccgc ataaacttta agtacaagag
    10681 gtccacgagc agtgtcaata agaatgactt tagtatcatc aatagaacga aaaactttta
    10741 atgttttaac cctgcaagtt aaaaagtcat ttagaacttg ctcataaaag ggatcattat
    10801 cttttatgaa aactttcaac ccatttatcg ttttattata tatcataatg aattatttta
    10861 accttaaatc tttagaagca tttttcttta taatacttta aataattaaa aatgcctgcc
    10921 atataatgct tttgtttaaa tttatgtttt gcacaatacc gatactgatt gctatttaca
    10981 ggtttaagta aatcttcttt actccacgga gaagctgctt tggcgatcaa aaaactacgt
    11041 gaaactggat aatcagccca ctcatgccag ggttttgtag gtcccacata gtgtataaac
    11101 acggtgtcat cattgactgg attatcaact ttgtctttta attcatagtt aatactatat
    11161 cgggtattat atttctccga aataaacttc actttaccat tcaataatac attcagtaca
    11221 tcttgatcaa ggtgggtgat tttacttacc caatctgggt cacgcaacat ttcaatagct
    11281 tttgacgaaa tgttatttag attccactca tcaatattta tcagtaaaaa accagcattg
    11341 aaatatccag aagctaactg tggtgtagtt aataccgagg ctcgattttg ccaccattca
    11401 atatctcttt cagctacaac tgcggcaatt tcattagtag aaaattgata atctaagagt
    11461 tctttaatac tacccttgca agcaatatct gcatcaagat atagtatttt ttcatgttta
    11521 tgatagaaat aatctgcaat tataaatcga aaatatgttg cgtaagtcca gtttttcgtg
    11581 cttggtaatg acttcagctt atcacaattg ataacataaa tattaattct taaattatat
    11641 tgttttgcta aatcagaaaa atataatttg tctttatcac tgatataatc tgtgaaaaca
    11701 tgaaattcac aagagatttc tctattgttt aatagaatag atgcgatggc tacaccacat
    11761 ccaaaaagaa aatttctgtc aataccaaat gctatatcaa gagtattttt agatcttata
    11821 ttttgataat gaaaattata ctcaaaaagg atgatgtcac tatcattgag ttgagacata
    11881 tttattctgt atccaaaatt ttggcaaaaa taagttacgg gttacaacga ttttcgaatc
    11941 gttctttccc tgatttttgt ggcttttgct tctgcttgcg acagcagtcc ttgttcctgc
    12001 ttgagaatgt ctttaagcgg ggcggcaaaa tacaccttca taaagcgcca gatatcccgc
    12061 tgagtcaggc caatattcat tgaagaaaaa taaagcccta taagatcttt atcgcgccaa
    12121 cgacgtggaa cgcgcgtgcg aagctgcgcc cggtgtaggt caattaccga aatttttaac
    12181 tcctcttcct taccggaaaa aggcaagtgc agcaggaaat gacagatata acagtcacga
    12241 tggttaatgc ccgcagcatg catatcgcgc accatcgtcg cgacacgctt aataagcata
    12301 cgctttacgc gaacatctgg tgggttggtc gcccagtcag cacagtaatc ttccagactg
    12361 atggttggtg tcagatcttc ggtaataata aacgaggttc tggtcagcgg attcatgcct
    12421 ttttcgccga atgccacccc atacatagta tcaacgccga catcccgcag tcgatgaatc
    12481 gcattccatt cgcggtctgc gcctaatact ggcatccgca atgagagtaa atttttgatt
    12541 atctctttca gggtcgtgcc gcgatgccat ttgagaaaat agctttttcc cgccatttca
    12601 aagcgcagag tacggcgagt ttccagttca cgaaatacct caccctgcaa ggttttaact
    12661 tcctcaaaag gatctttgcc gcgccataac gtggcaaacg gctctttaag ttcaaccatc
    12721 cagaccaccc gttatgatat ccgccgcttt ctctggcaga ctgtataaat cttgtgtatc
    12781 agcataatgt cgcgcatttt ccgcccaagc ctggcgcaat gaagattgcg ttaacgcttt
    12841 gcgtaaaatc tcattcaatg tttcctggcg gaatggctca gcaatagcct cgccgcaatt
    12901 agcgtcgaca atataatgcg catagccaca aacggcagtt gttagtaccg gtaatcctgc
    12961 agtaatcgct tccagcagca caattcccgc cgcttcctgg taggcaggat gcagtaataa
    13021 atccgccgcc gccattaatt ccgagacatc gttgcgcccc gagaagaagt gaacattact
    13081 gcgcacgccg cgtttttctg ccagtgcctc aaattttcgc ggtttatcct gcccaacaac
    13141 atatagcaat gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt caatggaacg
    13201 atcgacacct ttacgcgtga agtctgaacc gacctgcaac aataaatatt gttgttcggt
    13261 tattccattc ttcttacgga agatttcacg gctattggct ggctgctggc tatatttacg
    13321 atcaggataa atccccggtg gcagaatatg aaaacgctcc gcttcagtct gataatgttt
    13381 ctggaaatcg gcgatttgct tatctgtcag catcagcagc tgtgtcggct tgctctgttc
    13441 gaaggttgcc cgctcaaagg cggcataatg gcgataacgt gacgtcaggc gatagaaaaa
    13501 gcctttttcc tgcgctactt tctcggcata acaaacatca gcggcataat aaacgtccag
    13561 ccccggcatt ttgttgaatc caacgacttt atcgacggga tgttcgcgta aatgtttttg
    13621 cacccaggca aaatactccg cattgcgccc gtgattggta tgcgatttaa ccggcacttt
    13681 gatcagttca aatacatcag ggcattcgcc ttcccacgac tgggtataaa cccgaacatg
    13741 atgacctcgg gcggcgactg tctgagcaat acgcataaaa tcgcgctgca aaccgccaaa
    13801 gggaaaatat ttatataaac aaaaagcaac gatcataacg acgtacctgt cgtggaggag
    13861 ggcagtaatt tatcgacagc agcaatgaca tctgccgccg gaataaccga aagatacatc
    13921 tcatttcggt cacgctgatc gcgcgttggc atttcccggt aatctcccgc ccagaattga
    13981 atcatgttat ttgaccaggg acgccagaaa atatgatctg ttgcgccaaa cagcgatatc
    14041 agcggcgtat taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc aataaacagc
    14101 tgcgcatgat cgattaacgc accaagttcc gggaaggtca cctttccagc cagcgccgtt
    14161 actggtggcg tctggcatcc ctgcgcaatt tcattgacgc aggccagatc gtctttatct
    14221 gggccggacg tcagaacgac ttcataacca cgagcatgta aggcatcaat cacagcggaa
    14281 aacttggcgt tgtcccagca tttgaagatt tgccgcgccg taggttggat aaccacataa
    14341 ttttgtccaa caccagcgtg atcaagttcg cgacgcatac gtttccagct tgcaggcggg
    14401 taactcatgg ttgtctgctt caccaacgaa tcaagtccca atggtgtcag cacggataag
    14461 ttactttcca ccacatttcc atcctgcaac ggcaccaaat gggtgaaact tttacgccaa
    14521 aaagcagact gccgatgatg ataatcctgg gaaattttca cacgagcatt taataagcga
    14581 accagtatag caaccatcca ttgatcggta agattgacga taaggtcata cttattggca
    14641 cgtaatacct tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt tgctttttta
    14701 ttttttatgc cgtagagcgc gttaatctct ggattttcag acaggatcgg gatggtgtcc
    14761 tgataaagca gcacatcgat ttttgcgtca aggtaatttt ttttcagcga actaatgacg
    14821 ggagtagtta ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat tcttcgaaat
    14881 ggcttatcca caattaactc tttttgttga gattatccga ttaaggtgaa gcagaaaaag
    14941 cacgctaccg ccccaggctc aacagctacc tgaatactga taccgcactg atattgacgc
    15001 gctagtgtat cacttctttc gcggcaatcc gacccgaatg atttttaagc acattgttga
    15061 ttttcaataa agaaatcaat caaataaaat cattagcaac catattcttt cactgttgct
    15121 ctgcgattcc ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa tacaaaaaaa
    15181 gtatagatgt tcagcaagaa ttggcgggta aattgctatt taagtcaaaa acgggaaaag
    15241 taatggtaaa gccacagcta aatacataga atccccagca catccataag tcagctattt
    15301 actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata
    15361 cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac
    15421 ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt
    15481 gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg
    15541 attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag
    15601 gatgttcagc acgtttatct gccgtatgac ctgcccgatg cgctcaaccg tttcctgaat
    15661 aaagtcgacc ccaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg
    15721 gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg
    15781 gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg
    15841 attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac
    15901 caggtaaccg ttaccggtag cctgaaattc gatatttctg taactccgca gttggctgct
    15961 aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc
    16021 actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc
    16081 ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac
    16141 cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt cccctccacc
    16201 agcacgcagg ttgtggttgg cgatacgatg ggcgagttga tgttactata cggcattgcc
    16261 gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct
    16321 gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc
    16381 gcgcggctgg agcaggcaag cgggcttatt accgttaccg atgccactac gcttgcaaaa
    16441 gaggtttcct ctttactcac cgacgccgtt taccgtagtt tctatggccg tcatgccgtt
    16501 gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac
    16561 ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt
    16621 cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaga tgttcgatca
    16681 cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg
    16741 tgtggcactg gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag
    16801 tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg
    16861 tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc
    16921 ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt
    16981 ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca
    17041 tcaggcgctg atggcaaaat tagcgtagcg tttatgccgg atggtatgcc atccggctcg
    17101 catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc
    17161 cacaatcggc gtaccgcaca cccgacacgg ctcacctttt cgcccgtaaa cctgcaattc
    17221 ctgcgcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc
    17281 ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc
    17341 cagcaacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct
    17401 ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagccacg gtttaatcgc
    17461 cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg
    17521 ctccggtcca agatgggcca gcacattatg cccttccagc tctttggtcc acagccaggc
    17581 accaaagcgg cgcggatcgg tgtagcgcag cactttaccg ttgctcatca ccaaatccac
    17641 atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc
    17701 caaatgaatg ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg
    17761 cacgctaagc actggttgat cgcttaatcg gtagatctct tctgaaaccg gccagcgcaa
    17821 gcgtccgttg cgcaccaccg catgaagaat ggttgcacca acgagatgcg gttctatgcc
    17881 gcgacggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg
    17941 caatcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt ttttcgttac
    18001 atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctga cgaacaactg
    18061 gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat
    18121 agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca
    18181 tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac
    18241 tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac
    18301 aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg
    18361 tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac
    18421 ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg
    18481 gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat
    18541 tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag
    18601 cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat
    18661 ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac
    18721 gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt
    18781 actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat
    18841 ttttatcgct tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga
    18901 aaaaagacgg ctatgcgtta taacccggtg ttgtgagtcg agaaagatca ccataaagat
    18961 ctcccgctcc tcacccgtga gctggctttg taaaaattcc cgcgtcatct ccgggctgag
    19021 taaagggctt tcttcacgca tccgcacgtt gtagtaacgt cgcgccagtt cagcaatccc
    19081 ctttaactgg gcaaatttcg ctacgccaat tccatgaaca ccactaaatt gttcatattc
    19141 agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa
    19201 tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa
    19261 ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg cattgttttt
    19321 caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgttccgtta aatcgacggc
    19381 cagttttcaa tcctggaaag agcctcgcaa agtgaatctc aggtgaaggc acgacaacaa
    19441 aagtattgtg ataaaatcgc caacttctgg tgtcacccaa caggaaaaat catcatgagc
    19501 ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct
    19561 gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg
    19621 gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac
    19681 agtctgcttg acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct
    19741 gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtgttgc tgccggaatg
    19801 gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc
    19861 gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct
    19921 tcccgtggtt tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt
    19981 cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc
    20041 gtcaacgacc tgaaacatct gaacattatg attaccgccg gtccgacgcg tgaaccgctc
    20101 gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc
    20161 gctgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccagtttc actaccgacg
    20221 ccaccgtttg ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagc cgccgtgaat
    20281 gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca
    20341 gctaccgtgg ccccagaaaa aatcaaaaag caggccacgc agggtgatga attaacaata
    20401 aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc
    20461 tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt
    20521 atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt
    20581 aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt
    20641 gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa
    20701 aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta
    20761 tgccacctct ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact
    20821 ggctcagggt gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc
    20881 actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg
    20941 taacctggta ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa
    21001 ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt
    21061 tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga
    21121 aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca
    21181 tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt
    21241 caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa
    21301 tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg
    21361 caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta
    21421 agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca
    21481 acctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc
    21541 ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa
    21601 tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc
    21661 agctgcgcca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg
    21721 aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc
    21781 gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgccg
    21841 cgcagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca
    21901 aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc
    21961 agataggcaa tcagatcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc
    22021 tcaacttcct gaatcgccga aatctcgccg cgcccgcgtt cctgacgatc gagcgaaatc
    22081 aacacgccag caagcgtcgc gccattggcc tgaataatct ccatcgactc gcgaatcgcc
    22141 gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc
    22201 agattgccgc cttcaccgtg gtcttttgct tctttgcggt taaagcagta cggcaggtcc
    22261 aagtcgtgat gctccgccag tgccacagcg gttgtggtgg caatcgggat ccctttgtaa
    22321 gcagggccaa acagcagatc gaactcaatg ccggaatcca ccaacgcttc agcgtaaaaa
    22381 cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg
    22441 cttttgcgcc cggatttcag cgtaaactcg ccaaacttta acacctgctt gctaagcgca
    22501 aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac
    22561 agacaaaaaa aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct
    22621 gcgtcgctac aatggattcg attccccctc gggccagcgc caacaaggtg agtagctctt
    22681 catgggtgaa cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg
    22741 tcatcactac gttcatgtcg gtctctgcgg ctgagtcttc aacgtattcc agatcgcaaa
    22801 ttgcttcacc gtttacaata ccgacagaaa ctgcggctac catccctttc atcgggttgg
    22861 ttttcagctt gccgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac
    22921 ccgtaatcga cgcggtacga gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa
    22981 tggtgaactc acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg gcgatcagac
    23041 gctggatttc cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg
    23101 tgtgggtaga acgtggcagc atgccgtact ctgcggtgat ccagccctgg ccctgacctt
    23161 tcaggaagcg cggcacgcct tcttcaatag aggcggtaca caatactttg gtatcgccaa
    23221 attcgaccag caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac
    23281 gcacctgatt attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat
    23341 gtggctgcgc attatacgga cttccggcgg ttattcctat cctgacaagg catcgatggc
    23401 tataatcctt ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc
    23461 ctacgcccgg cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt
    23521 aaaccagcgt tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc
    23581 tgtcgttcgc gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg
    23641 ctatgagcca gatgttagcg cacaaggtga gctgatcctc aatgaaaaac tggctaaaca
    23701 gctggtaact gccgcgaact gggtaaaaat gcagagtgac gaaggggaaa tcaacccggt
    23761 tgatattcta cgctggccgg gcgtgatggc agcccaggag caggatctcg acgcgattgc
    23821 cgctgaaatt ctcgcggcgt tggatggtac gctggacgac tttatcgtcg cgcgcgaaac
    23881 cgaaggtcag gcactgaaag cattgatcga gcagcgtctg gaaggcgtca ccgccgaagt
    23941 ggtcaaagtc cgcgcccata tgccggaaat tatgcaatgg cagcgtgagc gtctggtcgc
    24001 gaagctggaa gatgctcagg ttcagttgga aaacaaccgt ctggagcagg agttggttct
    24061 gctggcacaa cgaattgacg ttgccgaaga gctggaccgc ctcgaagcgc atgttaaaga
    24121 gacgtacaac attctgaaga aaaaagaagc ggttggtcgt cgtctggact ttatgatgca
    24181 ggagttcaac cgtgagtcga acactcttgc gtcgaaatct atcaatgccg aagtgacaaa
    24241 ctccgccatc gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatattga
    24301 gtaactcgta acaccaattc tgacttttca gccttcacaa atgccgccca ctcaaacaga
    24361 gcggcatttt tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc agattttgcg
    24421 atgttttttt gcccagcaca ctgagaacgt gagatactca caactgtata taaatacagt
    24481 tacagattta ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc
    24541 aaccttttga agagataaaa ctgattaatg caaactgaac cgccccggg
//
LOCUS       DADLSE010000050        23859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLSE010000050 DADLSE010000000
VERSION     DADLSE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.050"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1095
                     /locus_tag="JN692_002005"
     CDS             <1..1095
                     /locus_tag="JN692_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7264325.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLNVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            1053..1448
                     /locus_tag="JN692_002006"
     CDS             1053..1448
                     /locus_tag="JN692_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7264326.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            1468..2058
                     /gene="diaA"
                     /locus_tag="JN692_002007"
     CDS             1468..2058
                     /gene="diaA"
                     /locus_tag="JN692_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7264327.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            2068..2643
                     /gene="yraP"
                     /locus_tag="JN692_002008"
     CDS             2068..2643
                     /gene="yraP"
                     /locus_tag="JN692_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7264328.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(2757..3797)
                     /locus_tag="JN692_002009"
     CDS             complement(2757..3797)
                     /locus_tag="JN692_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7264329.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVTMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(3870..4505)
                     /locus_tag="JN692_002010"
     CDS             complement(3870..4505)
                     /locus_tag="JN692_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7264330.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            4633..5151
                     /gene="yhbO"
                     /locus_tag="JN692_002011"
     CDS             4633..5151
                     /gene="yhbO"
                     /locus_tag="JN692_002011"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7264331.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLATSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(5131..5563)
                     /locus_tag="JN692_002012"
                     /pseudo
     CDS             complement(5131..5563)
                     /locus_tag="JN692_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
     gene            5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN692_002013"
     CDS             5614..5916
                     /gene="yhbQ"
                     /locus_tag="JN692_002013"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7264332.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(5903..6406)
                     /locus_tag="JN692_002014"
     CDS             complement(5903..6406)
                     /locus_tag="JN692_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7264333.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(6400..6924)
                     /locus_tag="JN692_002015"
     CDS             complement(6400..6924)
                     /locus_tag="JN692_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7264334.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            7133..8128
                     /locus_tag="JN692_002016"
     CDS             7133..8128
                     /locus_tag="JN692_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7264335.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDDYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            8137..9015
                     /locus_tag="JN692_002017"
     CDS             8137..9015
                     /locus_tag="JN692_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7264336.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            9096..10103
                     /locus_tag="JN692_002018"
     CDS             9096..10103
                     /locus_tag="JN692_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HAY7264337.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN692_002019"
     CDS             complement(10221..11465)
                     /gene="mtr"
                     /locus_tag="JN692_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7264338.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(11619..13508)
                     /locus_tag="JN692_002020"
     CDS             complement(11619..13508)
                     /locus_tag="JN692_002020"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7264339.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGECREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN692_002021"
     CDS             complement(13501..13581)
                     /gene="yrbN"
                     /locus_tag="JN692_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7264340.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN692_002022"
     CDS             complement(13688..14572)
                     /gene="nlpI"
                     /locus_tag="JN692_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7264341.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN692_002023"
     CDS             complement(14681..16816)
                     /gene="pnp"
                     /locus_tag="JN692_002023"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7264342.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGCV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN692_002024"
     CDS             complement(17063..17332)
                     /gene="rpsO"
                     /locus_tag="JN692_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7264343.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN692_002025"
     CDS             complement(17481..18425)
                     /gene="truB"
                     /locus_tag="JN692_002025"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7264344.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDSLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LCELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN692_002026"
     CDS             complement(18425..18826)
                     /gene="rbfA"
                     /locus_tag="JN692_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7264345.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN692_002027"
     CDS             complement(18990..21662)
                     /gene="infB"
                     /locus_tag="JN692_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7264346.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNILGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN692_002028"
     CDS             complement(21687..23174)
                     /gene="nusA"
                     /locus_tag="JN692_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7264347.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN692_002029"
     CDS             complement(23202..23654)
                     /gene="rimP"
                     /locus_tag="JN692_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7264348.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         5628 a         6661 c         6001 g         5569 t
ORIGIN      
        1 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag
       61 ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca
      121 tccgcagagc tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
      181 gttcagcagg atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag
      241 ttgctgaaga gcaacacccc gctgaacgta ctggcactga accagccgga gaatatcgaa
      301 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
      361 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagtgcattg
      421 ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga aactgggcgg cggcaccgtt
      481 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
      541 attgctttaa cgggtagccc gattactccc agagcgacaa ccgactccgg catgacgacc
      601 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
      661 gtcgatgcgg tctacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc
      721 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagttcccg cagcgcacaa
      781 ggcaccgcag gcccggattt ccgactggag atggaaggct tgcagtacag cgaaatcccg
      841 atgctggcag gcggtaatct gccgttaatg cagcaggcgc tcagcgcggt gaacaacgat
      901 tattcgctgg ctcgcatgta tgcgatgggc gttgatgcct ggtcgttggc aaatcatttc
      961 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag tctgacggct
     1021 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     1081 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagcacaa gcgcgtcgct
     1141 ggctggaagg caaaggactg cggtttatcg ccgctaacgt gaacgagcgt ggcggcgaga
     1201 tcgatctgat aatgcgtgaa ggcctgacca ccgtttttgt cgaggtacgc tatcgccgct
     1261 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     1321 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     1381 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     1441 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     1501 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc
     1561 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     1621 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     1681 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     1741 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     1801 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc
     1861 gccgttacgc gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca
     1921 ggtttgttag ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     1981 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     2041 cctcaccagg atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt
     2101 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt
     2161 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     2221 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat
     2281 gtcacggcct atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg
     2341 gctcgcgcca aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt
     2401 cgtcagggcc agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa
     2461 gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     2521 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     2581 gatattgcca gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa
     2641 tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt cttatcatgc ctacaacgat
     2701 gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag
     2761 aacaacagcg ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc
     2821 cataaggctt tcgccgggaa cgctttgcgc agcataatca gtgacggcaa actcactgcc
     2881 gggagcgtca tcaacaatgc cagcgccgga gcggttccca tacctgccag catcatcgtt
     2941 tgtacaatcg gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc
     3001 gtcaccaccc acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg
     3061 cgtgcggcac ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagt
     3121 gtccatagcg ccctgcccca gcggctaaaa aaaccgctct gtgcttccgg tatgtcaatt
     3181 tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc
     3241 agcaacacca tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca
     3301 aaccccataa acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc
     3361 gcaccgccca tcgatacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag
     3421 gtacacatca tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat
     3481 tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg
     3541 atcaccccca gcaccgccgc tttccatact gcgaggaaat agatcatcgc gtaatccaac
     3601 gccgcttgcc acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt
     3661 tcggctgcag taaacgcttt gccgtagtaa ggttgccatt tcacatacca gagaccggca
     3721 atgacaacga gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat
     3781 gaagactgac cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata
     3841 ttacgctcac tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag
     3901 cttaaaatcg tcacgccttc atgctcaggc cgcatcgcct ctgccagcat gacccgcgcc
     3961 acgtctctcg catcaatgga tttccagtta cctggtaaca aacggaacag cggcgcaaaa
     4021 agcgtttcgt tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg
     4081 gtcaatttcg gccagttctg ggcaattaat gcttcttcca tctccccttt gacgcggtta
     4141 tagaaaaacg gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg
     4201 cccagtcgcc gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac
     4261 gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg
     4321 ggttcggtga cctgcgccaa cgcgtcactc agttgcggat catggggatt aaacaccccg
     4381 ggcatatcgc ccaacggacg tcgcgtcggc gcagcaatgg cgttaacttt cggttcatta
     4441 atcaacatcc gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga
     4501 ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc cccggctcat gcaccagtct
     4561 taatagacca tgaggtaatt atccccgatt gtggaaaatt cgcctcatcc aatgcaacaa
     4621 cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaagattca
     4681 gaatttactt cacccgcaga cgagttccgt aaagccggac acgaagtgat tactattgaa
     4741 aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa
     4801 tccatcgatg aagtgacgcc agcggagttt gatgccctgt tgctaccggg tggtcattca
     4861 ccggattatc tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaatagc
     4921 ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt
     4981 cgggggcgca aactgaccgc agttaaaccg atcattattg atgttaaaaa tgcgggcgtg
     5041 gaattttacg atcaggaagt cgtggttgat aaagatcagc tggctaccag ccggacaccg
     5101 gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agtcgcgtag
     5161 ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg
     5221 gatttcccac accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt
     5281 gcgcgcgagg tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacacc
     5341 gcgaattaac gctaccgttt tcggctgacc gtttaccgtt agcctgcggc ccactcatct
     5401 gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc gcatcaaaaa
     5461 gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag gtgacaacat
     5521 gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt ctcttttata
     5581 ctgtgggcct gaccttaaca tgcgatgcag acgatgacac cctggtttct ttacttgatc
     5641 cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg ccgctatcag
     5701 cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact gacgctggcg
     5761 ttttctgcgc cagtgggcga ccgttcgctg gcactacggg cggaatatcg cgttaagcaa
     5821 ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc agaactgttg
     5881 agcagcctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac tcaaccagac
     5941 cggttacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg ctttcagtgc
     6001 ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa cgactgtaca
     6061 gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc gaatcgagtc
     6121 cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca accgccaacg
     6181 gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta aatgccacat
     6241 agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga aagccatctt
     6301 cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga cgcagcaggg
     6361 catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt cacctaccga
     6421 tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg ccagttgcag
     6481 cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca tcaggttttt
     6541 cacatacacc cccagctccg tatcgccttc aatcaccagc cgacgctgga agaagagcgt
     6601 atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg cactaaaact
     6661 cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg aggtaaacca
     6721 ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa actccagctc
     6781 gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa cctggcgttt
     6841 tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca aatgcacaat
     6901 acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc atcctatttt
     6961 gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa attgctgcta
     7021 ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt taactaaaat
     7081 cccagttcgt taacattttt acgttttgat agcgcaacct tcaggaaaaa ttatggagct
     7141 gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg gcgcagatgc
     7201 tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc ttaactttac
     7261 cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca aacttcacat
     7321 cgcgattaac acttttgcgc atccggacga ttacgcccgt tggcagcgtg ccgtggatat
     7381 ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc tggagtacgc
     7441 cgccgagcgt tacccgcata ttgaacgtca tgtgtcagtg caggcttcgg cgaccaatga
     7501 agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc tgccgcgcgt
     7561 gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc tggaagtgtt
     7621 tgctttcggc agcctgtgca ttatgtcgga aggccgttgc tatctgtcgt cgtatctgac
     7681 gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc gctggcaaca
     7741 aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt atcaggacgg
     7801 cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg gcgagcgcta
     7861 tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg agttaatggc
     7921 tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt atgtcagcca
     7981 ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc aaaacttcgt
     8041 accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc aaaccaccct
     8101 tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt agggccagtg
     8161 ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaggccgc cgccagcagc
     8221 gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gccgggcaac caaagttggc
     8281 gactgtctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt gctctccacg
     8341 ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt tgagaacggt
     8401 gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga acgcaaactg
     8461 ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa aatattgctc
     8521 aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg gctggtgaat
     8581 ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga agtcctgagc
     8641 tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc ggaagaccgc
     8701 ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa cgtgctgtcg
     8761 caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg ctacgtttac
     8821 aacctcggta acgagctggc atccatgcag ggattggttg atgtagtccg cctgtcaccg
     8881 cagggcactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa tggcgctgtg
     8941 ccactgccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct ggcaggactg
     9001 gagctgcaag cctaagtaaa tagctcactt tgttaacaac tttaactatt ctttaatgca
     9061 gtattaaaga ttaatcggta acaaagtgag ctgttatgac tgataaaacc attgcgtttt
     9121 cgctactcga tctggccccc atacccgaag gctcttcagc gcgggaagcg ttctcccact
     9181 ctctcgatct cgcccgtctg gctgaaaagc gcggctatca tcgctactgg ctggcagaac
     9241 accacaatat gaccggcatt gccagtgctg caacatcggt attgattggc tatctggcgg
     9301 cgaataccac cacgctgcat ctggggtctg gcggcgtgat gttgcctaac cactcaccgt
     9361 tggtcattgc cgaacagttc ggcacactca atacgctcta tccggggcga atcgatttgg
     9421 ggctgggtcg tgctccgggt agtgaccaac ggacaatgat ggcgctacgt cgtcatatga
     9481 gcggcgatat tgataatttc ccccgcgatg tcgcggagct ggtggactgg tttgacgccc
     9541 gcgatcccaa tccgcatgtg cgcccggtac caggctatgg cgagaaaatc cccgtgtggt
     9601 tgttaggctc cagcctttac agtgcacaac tggcggcgca gcttggtctg ccatttgcgt
     9661 ttgcctcaca cttcgcgccg gatatgttgt tccaggcgct gcatctttat cgcagtaact
     9721 tcaaaccgtc ggctcggctg gaaaaaccat acgcgatggt gtgcatcaat attatcgccg
     9781 ccgacagcaa ccgcgacgct gaatttctgt ttacctcaat gcagcaagcc tttgtgaagc
     9841 tgcgccgtgg cgaaaccggg caactgccgc cgccgattca aaatatggat cagttctggt
     9901 caccgtctga gcagtatggc gtgcagcagg cgctgagtat gtcgttggta ggcgataaag
     9961 cgaaagtgcg tcatggtttg cagtcgatcc tgcgcgaaac cgacgctgat gagattatgg
    10021 ttaacgggca gattttcgac caccaggcgc gcctgcattc gtttgagctg gcgatggatg
    10081 ttaaggaaga gttgttggga tagtgtgtct taacgcggga agccttatcc gagctggcaa
    10141 cgctgtccta cgtaggcctg ataagcgaag cgcatcaggc attgtgtagg cagcagaaat
    10201 gtcggataag gcaccgctga ttactgatac accggcagta aattaaagct cgataaaata
    10261 tgcaccagtg cgttgccgac gccaaacacc agaatcagcg caatcatcgg cttgccaccc
    10321 cagacgcgga atttcgggct gccaaagcgt ttacgcgatg cgcgggccag cagtgccgga
    10381 acaattgccg cccagatggt agccgctaaa ccagcataac caatggcata caggaatccg
    10441 ttcgggaaca gcagaccacc cacaacaggc ggggcaaagg tcagcaatgc cgttttcaag
    10501 cggcccacag ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa caaacccagc
    10561 gttacgccaa ggaacgaact cgctaccgca aagtttgaga acacgaccag cagaaggtcc
    10621 aggctacggc tgttcagcac gccgcttaat gcttgtacca gcacatcaat attaccgccc
    10681 ttctcggcga tgccgataaa ctccggacga gggatgttgc ccattgtcgc cagcaaccag
    10741 atggtataca gcgccagtgc catcagcgta ccgtacacca ggcatttcac gatggttttc
    10801 ggatctttgc cgtaatactt catcaggctt ggcacgttac cgtgataacc aaacgacgcc
    10861 aggcagaacg gcagggtcat cagcaaatac ggcgcataag atgcattgct ttcggcaacg
    10921 ttgaacaatg tcgcaggctg cacatgcccc agcaggctac cgaaggtgag gaagaaggta
    10981 atgactttcg cccccagcac aatcgccgtc atgcggctga cggctttagt actcaaccac
    11041 accacaaacg ctaccagcag tgcaaaacca aaacccgccg cccgtgccgg gacgtttagc
    11101 gacatctctg cgaaggtgtg atgcagaatc gaaccactgg cagaaatata ggcataggtc
    11161 aggatataga gcacaaaggc aatggaaatg ccgttgacca cgttccagcc tttgcccagc
    11221 aaatctttgg tgatggtgtc aaaactcgaa ccgattcggt aattcaggtt agcttccaga
    11281 atcatcaagc cggaatgcag catacagaac caggtaaaga tcagcgctgc cattgaccag
    11341 aaaaaccacg ccccggacat gaccactggc agagaaaaca tccctgcgcc aataatggtg
    11401 ccgccgataa tcaccacgcc gccaagcagc gacggtgacg tttgggtggt ggttagtgtt
    11461 gccatgaggg cttctctcca gtgaaaaata gtgcgactgc gttgttatgc attgcactgt
    11521 accagtacat gagtacaaaa gacagaaaaa aagccccgat ggttaaaatc ggggctgtat
    11581 atattatttt acagattgtg ttcgctgttc agcgatgatt acgcatcacc accgaaacga
    11641 cgacgaccgg tagagtcatc acgacgcggt gcgcggcctt cacgacgttc gccgctaaaa
    11701 cgacgaccat caccacgacc accttcacgg cattcaccgc tgaagttacg accgccttca
    11761 cgacgctcgc caccgaaacc acgaccaccg ccacgacgct caccgccagt atgcggctgt
    11821 gcatcgccca gtaactgcat gttcatcggc ttgttgagaa tgcgagtgcg cgtaaagtgt
    11881 tgcagcactt cacccggcat acctttcggc agttcgatgg tggagtgaga agcaaacagc
    11941 ttgatgttac cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc gccaacgata
    12001 tgacgaactt caacaccatc atcgcggccc acttcaatgc ggtacagctg catatcgcca
    12061 acatcacgac gttcacgacg cggacgatct tcacggtcgc cacgcgggcc acggtcgtta
    12121 cgatcgcgcg gaccacggtc atcacggtca cggaattcac gtttcggacg catcggcgca
    12181 tctggcggaa caatcagagt acgttcaccc tgtgccattt tcagcagtgc cgcagccaga
    12241 gtttcgagat ccagctcttc accttctgca gtcggctgaa ttttgctcag cagtgcgcgg
    12301 tattgatcca gatcgctgct ttccagctgc tgctgtactt tagcggcgaa tttttccaga
    12361 cggcgtttgc ccagcagttc tgcgttcggc agttctactt ccggaatagt cagcttcata
    12421 gtacgttcaa tgttgcgcag cagacgacgc tcgcggttct caacgaacag cagcgcgcgg
    12481 ccagcacgac ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc agaatccatc
    12541 gggatatcgt agttaaccac caggctgata cgctcaacgt ccaggccacg ggccgcaacg
    12601 tcggtcgcaa tcaggatgtc cagacgaccg tctttaaggc gttccagtgt ctgctcacgc
    12661 agcgcctggt tcatgtcacc gttcagcgcg gcgctattgt agccgttacg ctcaagagct
    12721 tcagccactt ccagagtcgc gtttttggta cgaacgaaga taatcgccgc atcaaaatct
    12781 tccgcttcca ggaaacgtac cagtgcttcg tttttacgca taccccacac agtccagtag
    12841 ctctggctga tgtcaggacg ggtagtcacg ctggactgaa tacgcacttc ctgcggctct
    12901 ttcataaagc ggcgggtaat gcgacgaatc gcttccggca tggttgcaga gaacagagcg
    12961 gtctgatgac cttccgggat ctgcgccata atggtttcaa cgtcttcgat gaagcccatg
    13021 cgcagcattt cgtcagcttc atccagaacc agaccgctca gtttagagag gtccagagtg
    13081 ccacgtttca ggtggtccag cagacggccc ggagtaccga caacgatctg cggcccctga
    13141 cgcagggcgc gtaattgcac gtcataacgc tggccgccgt acagggcaac cacatttacg
    13201 ccgcgcatgt gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc cagttcgcgg
    13261 gtcggtgcca gcaccagaat ctgtggtgct ttcagctcag gatcaagatt ctgcagcaga
    13321 ggtaaagaga atgctgcagt ttttccgctc cccgtctggg ccatacccag aacgtcgcgg
    13381 ccattcagca gatgtggaat acactctgcc tgaattggag atggtttttc gtaacccaga
    13441 tcgttaaggg cttcaaggat aggagccttc aggcccagat ctgcaaaagt ggtttcgaat
    13501 tcagccatgt agtacgtgtg cctcaaaatt aatggcggcc agtctacata actcatcatg
    13561 aaattgatca gcaattttca ttgaaaagtg tgaaccggct caaagtaggt gtattaacga
    13621 acaacaacgc cctcacccgt taaggtgatg gcaatcaaaa aagattacgg gctgatgtgt
    13681 acgtcagcta ttgctggtcc gattctgcca ggtcatcttg gtcctggccc aggagcgata
    13741 attccaacaa tgcgtatcgg tgctcaacaa agttatgaac gttgttggca accgccagtt
    13801 tgaacagtgc cgtggcgctg tccaaatccc ccagacttag gtagtactta cctaaataga
    13861 agttggtttc actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc gccttgagcc
    13921 tttccattaa cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg ttccatcccc
    13981 actgttcctt atccgatttt tcgaagtgct gtttcaacac ttctttagcc tgcttctcat
    14041 cgagcttctg ctcggcgaga taaagccaca gactacggaa aggatcattg ggatcgtctt
    14101 gataaaacgc cagcagatca tcttgcgcta acttgtcacg accgccgtaa tataatgcga
    14161 tcccgcgatt caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca gaatcaaacg
    14221 cttcataggc agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag taattgaata
    14281 cttcaggcat atccggtcgg attgccagcg cttgcgaaaa atcgttacgc gctaatgccc
    14341 tcagaccgag actatcatac aacactccgc gctcatataa aagctgtgcg cgttcgtcat
    14401 cggttaaagc ccgactggca aggatttgtt ccatacgtgc cagaatcact tcctgctgta
    14461 aagtcggttg caatggtacc gcgaggactt cacttttacg ccaggaagta ttactgcatc
    14521 ctgcaagcgt aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc atttcccact
    14581 cccgaagacc acggttgaat gaacgtcctg ttcccggttg ctaacaaggc gtcctgcccg
    14641 gttaaaagcc ccccgccgca gcggagggca aatggcaacc ttactcgccc tgttcagcag
    14701 ccggagcttc cggtgctgca gcaggttgag actgctcagt cgcttcttta atgctcagac
    14761 ggatacggcc ctggcgatca acttccagaa ctttcaccgg tacttcctga cccatctgca
    14821 ggtaatcggt cactttctca acgcgtttgt cagcgatttg agagatgtgt accagacctt
    14881 ctttaccgcc gccgatggca acaaatgcgc caaagtcaac gatacgggtc actttaccag
    14941 tgtagacgca gcccacttcg atttctgcag tgatctcttc gatacgacga atagcatgtt
    15001 tcgctttctc gccgtcggtc gctgcaatct tcacagtacc gtcatcttcg atttcgatgg
    15061 tggtgccagt ttcttcggtc agggcacgga taacagagcc gcctttaccg ataacgtctt
    15121 tgatcttgtc cgggttgatc ttgatggtat ggatacgcgg tgcgaactca gagatatcgc
    15181 cacgcggcgc gttgatcgcc tgttccatta cgcccaggat atgcagacgc gcacctttag
    15241 cctggttcag cgcaacctgc atgatctctt tggtgatacc ttcaattttg atatccatct
    15301 gcaatgcaga gataccctcg cgggaacccg caactttgaa gtccatatcg cccaggtgat
    15361 cttcgtcgcc caaaatgtca gacagtacaa cgtagttgtc gccttctttc accagaccca
    15421 ttgcgatacc cgcaacggca gctttgatcg gcacacctgc gtccatcagc gccagagacg
    15481 cgccgcacac ggaagccata gaagaggaac cgttggattc agtgatttca gacacaacac
    15541 gtacggtgta cgggaatttg tccatatccg gcatgactgc cagcacgccg cgcttcgcca
    15601 gacgaccgtg accaatttca cgacgcttcg gagaaccgac catgccggtt tcgcctacgg
    15661 agtacggagg gaagttgtag tggaacagga aggtatcggt acgttcgccc atcagttcat
    15721 caagaacctg cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc tgcgtttcac
    15781 cacgggtgaa cagcgcagaa ccgtgagtac gcggcagcac gccagtacgc acatccagac
    15841 cacggatcat atctttttca cgaccgtcga tacgcggttc gcctgccagt acgcggctac
    15901 gaacaacgtt tttctcgatc gcgtgcagaa tttcacccag ttcgttttcg tccagggttt
    15961 cgtcttcagc aagcagcgtc gcgatggttt cagatttgat gacatcaacc tgcgcataac
    16021 gctcttgttt gtcggtgatg cggtaagcat cgctcaggcg agcttcagcc agtgcagcaa
    16081 cgcgcgcgtt cagcgcttcg tttaccggct ccggctgcca gtcccaacgt ggtttaccgg
    16141 cttctttcac cagttcattg atgttctgaa taacaacctg ctgttgttca tgaccgaaca
    16201 ctactgcgcc cagcatctgg tcttcgctca gcagttcagc ttcagattca accatcagta
    16261 cagctgcttc agtaccggca acaaccagat ccagtttgct ctctttcagc tcgtcctgag
    16321 tcgggttcag tacgtactgg tcattgatgt aacctacgcg agcagcacca atcggaccat
    16381 tgaacggaat accagacaga gacagtgctg cggaagcacc aatcatcgcg acgatatccg
    16441 ggttaacttg cgggttaaca gaaaccacgg tggcgataac ctgaacttcg ttgacgaagc
    16501 cttccgggaa cagcgggcga atcgggcggt caatcagacg cgcgatcagg gtttcgcctt
    16561 cgcttgggcg gccttcacga cggaagaagc tacccgggat acgaccagca gcgtaggtac
    16621 gctcctgata gttaacggtc agtgggaaga agtcctgacc tggtttggct tttttctggc
    16681 caacaacggt aacaaatacc gcggtgtcat ccatgctaac cataacagcg gcagtagcct
    16741 gacgagccat catgccggtt tccagagtca cggtgtgttg gccgtactgg aatttacgaa
    16801 cgatcggatt aagcaatgta atatcctttc tctttcttag acagtacctt acggcactgg
    16861 tgttaatacc cgatcttctg cgcatcctcg cgactaatga caaccctaac ccagctctat
    16921 gtgggtaaag cctctcatta gccgcgcgaa cctctgcaac ggaagatcat tcatagcaac
    16981 aatacattag tttccagtga attgctgccg tcagcttgaa aaaaggggcc actcaggccc
    17041 ccttttctga aactcgcaag aattagcgac gcagacccag gcgctcgatg agctgggtgt
    17101 aacgtgctac gtctttacgt ttcaggtagt cgagcagttt acgacgctga gaaaccatgc
    17161 gcagcagacc acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg ccctgcaggt
    17221 ggttgatctg tgcagtcagc agtgctacct gaacttcagt agaaccggtg tcgtttgcgt
    17281 cacgaccaaa ctcagaaacg attttagctg ttgcttcagt acttagagac attttaaaac
    17341 tccaaagtat atagaatgaa aggacgccga tctctaattc agcgatccca gtgtacgtta
    17401 cgcaaagtgt taaacaattt acgcgacgtt aagcggcagt attctactcg tagcgacctg
    17461 ttatcgcaag acggttaaca ttacgccggg tattcaacca ccaggcgacg aggcgcaacg
    17521 cggccttcat cgtcaatttc gcccataccg ataaatttgc cgttctcacc ttctgtaacg
    17581 cgaaccagtc cttccagcgg cgcaccggat gtacgaactg ggttaccatt tttgaagtaa
    17641 acagaagacg ttaacggaag attcaccacc gggtagtccg aagctggact gtccattggc
    17701 atcagtaatg gatcaagtaa ctccgcagcc ggaatatcct gctgttcggc ttgttcgacc
    17761 agttcacaca gatgttccag ggtcaccatc cgttcaaccg gatatttact taccgccaga
    17821 cgacgcaggt aaataacatg cgcgccacaa ccgagttttt caccaaggtc atcaatgatg
    17881 gtgcggatat aagtgccttt tgagcagtga atttccagct ccagctcatt gccttcgtgg
    17941 cgaataaaca gcaattcata aacggtaatc gggcgcgctt cacgcggtac ttcaatgccc
    18001 tgacgcgcat attcgtacag ttttttaccc tgatatttca gtgctgaata catcgaaggg
    18061 atctgttcga tatcgccacg gaaagtatcc agcgccgccg ccagttgctc tgcgctaaag
    18121 gttaccggac gttcttcaac gatttgtccg tcggcatcag aagtatcggt acgctgtcca
    18181 agacgcgcaa tgacccgata gcgtttgtcg gagtccagca gatactggga aaacttcgtc
    18241 gcttccccga ggcaaattgg caacatgcca gttgccagcg agtccagcgc accggtatgc
    18301 ccggcacggt tggcgttata aatccgtttc actttttgca gcgcatcatt gctggacata
    18361 ccctgcggtt tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg acgaggacga
    18421 ctcattagtc ctccttgctg tcgtccgggt taacacgacg ttcttcgtca tgtttgacca
    18481 cgctggtcac caggtttgac atgcgcatcc cttcaaccag agagttgtcg tagaagaagg
    18541 tcagttccgg cacgatacgc aggcgcatcg ctttccccag caggctgcgg atgaaaccag
    18601 aagcttcttg caacgctttg atgcccgctt taaccgcgtc ttcatctttg tcgttgagga
    18661 acgtcacata tactttggca tacgccaggt cgcgagacat ttcgacaccg gaaacggtgg
    18721 tcatcatgcc caggcgagga tctttaattt cacgctgcag gatgagagcg atctcttttt
    18781 gcatttcctg cgctacgcgc tgcgggcgac caaattcttt cgccataata aattctcctg
    18841 acaaaaaagg ggctgttagc ccctttttaa aattaatttc aggtggaagg gctgttcacg
    18901 ttggcctgat aagacgcgcc agcgtcacat caggcaatcc atgccggatg cggcgtaaac
    18961 gccttatccc gcatggaacc ctaaaaacct taagcgatgg tacgttggat ctcgatgatt
    19021 tcgaatactt cgatcacatc gccagtgcgg acgtcgttgt agttcttaac gccgatacca
    19081 cattccatac cgttacggac ttcgttaacg tcatctttga agcggcgcag ggactccagc
    19141 tcgccttcgt agataaccac gttgtcacgc agaacgcgga tcgggttgtg acgtttaacc
    19201 acaccttcgg taaccataca gcctgcgatg gcaccaaatt tcggcgattt gaacacgtca
    19261 cgaacttccg ccagaccgat aatctgctgt ttcagttccg gagacagcat accgctcatc
    19321 gccgctttca cttcgtcaat caggttatag atgacggagt agtaacgcag atccaggctt
    19381 tccgcttcaa tcactttacg tgcagaggca tcagcacgta cgttgaagcc aaccaggatg
    19441 gcgttggacg ccgcagccag ggtggcgtcg gtttcggtga taccacctac gccagaaccg
    19501 atgatcttca ctttaacttc gtcagtagac agtttcagca aggagtcgga gatcgcttcc
    19561 acagaaccct gtacgtctgc cttcaggacg atgttcactt cgtgaacttc gccttcggtc
    19621 atgttggcga acatgttctc gagtttagat ttctgctgac gcgccagttt aacttcgcgg
    19681 aatttaccct gacgatacag tgcaacttca cgcgctttct tctcgtcacg tacaacggta
    19741 acttcatcac ccgcagccgg tacgccggac aggccgagga tttccaccgg aatggacgga
    19801 cccgcttcca gcacttcctg acccagttcg ttacgcatcg cacgaacacg accgtattcg
    19861 aagccacaca gaacgatatc gcccttgtgc agagtacctt cacgtaccag aacggtagca
    19921 accggaccac gacctttatc gaggaaggat tcgataaccg caccgctcgc cataccttta
    19981 cgtaccgctt tcagctccag aacttccgcc tgcagcagga tagcgtccag cagttcatcg
    20041 ataccagtac ccgctttcgc agacacatgt acgaactggc tttcaccgcc ccactcttcc
    20101 ggcaggatgc cgtactggga gagttcgttc ttaacgcgat ccggatcggc ttctggttta
    20161 tcgatcttgt tcactgcaac gaccaccgga acctgcgccg ctttcgcgtg ctggattgct
    20221 tcgatcgtct gcggcattac gccgtcgtcg gcagcaacaa ccaggactac gatgtccgtt
    20281 gcctgcgcac cacgagcacg cattgaagta aacgcggcgt gccccggggt gtccaggaag
    20341 gtgatcatgc cgttttcagt ttcaacgtgg tatgcaccaa tgtgctgggt aatgccgccc
    20401 gcttcgccag aggccacttt cgttgaacga atgtagtcca gcagagaggt tttaccgtgg
    20461 tcaacgtgac ccatgatggt cacaaccggc gcgcgcggtt cagccgcagc acccgtgtca
    20521 cggtcgctca ttaccgcctc ttccagctcg ttttcacgac gcaggataac tttatggccc
    20581 atctcttcag caaccagctg tgcggtttcc tgatcgataa cctggttgat ggttgccatt
    20641 gcgcccagtt tcatcatcgc tttgatgacc tgagagcctt taaccgccat cttgttcgcc
    20701 agttcgccaa cggtgatagt ttcgccgatc acaacgtcac ggttaacggc ctgagcaggc
    20761 ttctggaagc cttgctgcag cgaagaacct ttacgttttc cgcctttacc gccacgtact
    20821 gctgcgcgtg cttcttcacg atcagctttt gattcagcgt gtttgttgcc tttcttcgga
    20881 cgcgctgctt tcgcgttacg accacggcca cggccgcctt cgacttcacg atcgctttcg
    20941 tcttctgcct ggcgagcatg ttgagaagta gtgacgtgat aatcgctgga atcttcagtc
    21001 ggttccgcgt tatcagtcca tttgttttct tccgccatac gacgtgcttc ttcagcaacg
    21061 cgacgtgctt cttcttcgag tttacgacgc gcttcttctt cagctttacg cttgagctct
    21121 gcagcttcct gctcacggcg ggctttttca gcctgggcgt ttttagtcat atcgtcttgt
    21181 tgattgctca ctttgtcttt ttccgcagct tcacgtttcg cttgttcagc agcttcacgc
    21241 ttagcttgtt ctgcggcctc acgttcagct ttttgttgcg cctcgcgttt agccgattct
    21301 tctgcctcac gacgggcttg ctcttccgct tcacgctgcg cttgctcttc cgctgcaagg
    21361 cgttcagcct cttgcggatc gcgtttcaca aaggtgcgtt tcttgcggac ttcgatttgt
    21421 accgatttgc tttttccacc ggtaccaaga atgttaaggg tgctacgtgt tttacgttgc
    21481 agcgtcaatt tgtccgggcc tgaatttttc tgattcaggt ggtcaatcaa agtctgtttc
    21541 tcttgtgcag acacagagtc gtcagcagac ttccggatac ctgcatcagc aaattgctgt
    21601 accaggcgtt ccacggaggt ctgtcgctct gcggccagcg ttttaatcgt tacatctgtc
    21661 atgctgttcc ttcctgctac agtttattac gcttcgtcac cgaaccagca aatattacgg
    21721 gcagccataa tcagtgctcc ggctttttcg tcggtcaacc cttcgatatc agccagatca
    21781 tcaatgccct gttcggcgag atcttccagc gtacaaacgc cacgggcggc cagtttgaat
    21841 gccaaatcac gatctacccc ttcaaggttc agcagatcgt cagccggttt gttatcaccg
    21901 aggctttctt cctgggcctg tgcaatggtg gccagtgcat ttttagcacg ctcgcgcagt
    21961 gcttcaacgg tcggctcatc aaggccttcg atttccaaca gctctttcat cggcacatag
    22021 gccagttctt ccagcgtcga gaaaccttct tctaccagaa cagtcgcgaa gtcttcgtcg
    22081 atgtcgagat atttggtgaa ggtgtcgatc gctgcgtgcg cttccgcctg atgcttagcc
    22141 tgcaggtcgt caacggtcat tacgttgagt tcccaaccgc tcagctgcga agccagacgc
    22201 acgttctgac cgttacggcc aatcgcctgc gccaggttac cggcttcaac ggcgatatcc
    22261 atggtgtgtt tatcttcatc caccacgata gaagcaacgt ctgccggtgc cattgcgtta
    22321 atcacaaact gcgccgggtt atcatcccac agaacgatat cgatacgctc gccgcccagt
    22381 tcagtagaaa ccgcctgaac acgcgcgcca cgcataccta cgcaagcacc taccggatcg
    22441 atacgtttat cgttggtttt caccgcgatt ttcgcacgag aacccgggtc gcgagccgct
    22501 gctttaattt caatcacttc ttcgccgatt tccggcactt caatacggaa cagttcgatc
    22561 agcatttccg gcttggaacg agtgacgaac agttgcgcgc cacgcgcttc cgggcgaacg
    22621 gaataaagca cgccacgaac gcggtcgcca gggcggaagt tttcacgcgg cagcatatct
    22681 tcgcgcagga tcacggcttc agcgttgttg cccagatcca gagagatgtt gtcgcggttt
    22741 acttttttca ccacgccggt gatgatttca ccttcgtgtt cacggaactg atcaaccacc
    22801 atcgcacgtt cggcttcacg cactttctgc acgataacct gttttgccgt ctgggtggtg
    22861 atacggtcaa aggtaacaga ctcaatctga tcttcaacgt aatcgcccag gttcaggctt
    22921 tcatcttcat aacgtgcggc ttcaagggtg atttccttgg tcggctgggt gacttcatca
    22981 acaactaacc agcgacggaa ggtgtcaaaa tcaccgcttt tgcgatcgat ctgtacacgg
    23041 acgtcgatct cttgctcata ttttttcttt gttgctgtcg ccagcgcgct ttccaatgct
    23101 tcgaaaatct tctcgcgagg tagcgccttt tcattggata cggcttcaac tacagccaaa
    23161 atttctttgt tcatcgcggg cttttcacct catccagact attaaaagtg gggaaccagg
    23221 ttcgccttct ggatattact cagcgcgaac acttcatctt taccttcgac ggtaactgtg
    23281 atcatttcac cgtctaccgc tttgataacg ccctgccatt tacgacggtt ttgtaccgcc
    23341 atacggagaa ccagagtcac ctcttctccg acaaaacggg cgtagtgttc agccgtgaac
    23401 agtgggcgat cgagacccgg tgaggagact tccaggttat aagcaacggt gatgggatct
    23461 tcaacatcca gcacagcact tacctggtgg ctcacatcag cacaatcatc aacattgatg
    23521 ccatcttcac tatcaatata gatgcgcagt gtggatgtgc gaccgcgaat aaattcgatg
    23581 cctaccagtt caaaacccag ggcctcaact ggcgcagtaa tcatctctgt taatttttgc
    23641 tctaatgtgg acaagcccac ccccaagaca taaaaaaagg gcctaaagcc cagttattct
    23701 gtagtcagat aacaaaaaac cccgataaat cggggcttta tataactgaa ccctataacc
    23761 gcaactgcgg tctggagcac tttccagaag gattttttca aatcccacta cgaaggccga
    23821 agtcttcaca gtatatttga aaaaggactc taagggaaa
//
LOCUS       DADLSE010000051        23137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLSE010000051 DADLSE010000000
VERSION     DADLSE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.051"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN692_002030"
     CDS             complement(260..877)
                     /gene="tdk"
                     /locus_tag="JN692_002030"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7264349.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1482..1895
                     /gene="hns"
                     /locus_tag="JN692_002031"
     CDS             1482..1895
                     /gene="hns"
                     /locus_tag="JN692_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7264350.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN692_002032"
     CDS             complement(2039..2947)
                     /gene="galU"
                     /locus_tag="JN692_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7264351.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN692_002033"
     CDS             complement(3149..4162)
                     /gene="rssB"
                     /locus_tag="JN692_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7264352.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN692_002034"
     CDS             complement(4254..5159)
                     /gene="rssA"
                     /locus_tag="JN692_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7264353.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5272..5730
                     /locus_tag="JN692_002035"
     CDS             5272..5730
                     /locus_tag="JN692_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7264354.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5780..6622
                     /gene="purU"
                     /locus_tag="JN692_002036"
     CDS             5780..6622
                     /gene="purU"
                     /locus_tag="JN692_002036"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7264355.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6782..6866
                     /locus_tag="JN692_002037"
     tRNA            6782..6866
                     /locus_tag="JN692_002037"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6816..6818,aa:Tyr,seq:gta)
     gene            6913..7044
                     /locus_tag="JN692_002038"
     ncRNA           6913..7044
                     /ncRNA_class="other"
                     /locus_tag="JN692_002038"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN692_002039"
     CDS             complement(7228..7905)
                     /gene="narI"
                     /locus_tag="JN692_002039"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7264356.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN692_002040"
     CDS             complement(7905..8615)
                     /gene="narJ"
                     /locus_tag="JN692_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7264357.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDVVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN692_002041"
     CDS             complement(8612..10150)
                     /gene="narH"
                     /locus_tag="JN692_002041"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7264358.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10147..13890)
                     /locus_tag="JN692_002042"
     CDS             complement(10147..13890)
                     /locus_tag="JN692_002042"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7264359.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPIADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNSVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYVH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN692_002043"
     CDS             complement(14283..15674)
                     /gene="narK"
                     /locus_tag="JN692_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7264360.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16013..17809
                     /gene="narX"
                     /locus_tag="JN692_002044"
     CDS             16013..17809
                     /gene="narX"
                     /locus_tag="JN692_002044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7264361.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17802..18452
                     /gene="narL"
                     /locus_tag="JN692_002045"
     CDS             17802..18452
                     /gene="narL"
                     /locus_tag="JN692_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7264362.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN692_002046"
     CDS             complement(18453..19847)
                     /gene="ychO"
                     /locus_tag="JN692_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7264363.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20032..20385
                     /gene="ychN"
                     /locus_tag="JN692_002047"
     CDS             20032..20385
                     /gene="ychN"
                     /locus_tag="JN692_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7264364.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20429..21124)
                     /locus_tag="JN692_002048"
     CDS             complement(20429..21124)
                     /locus_tag="JN692_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7264365.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN692_002049"
     CDS             complement(21282..21512)
                     /gene="chaB"
                     /locus_tag="JN692_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7264366.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21782..22882
                     /gene="chaA"
                     /locus_tag="JN692_002050"
     CDS             21782..22882
                     /gene="chaA"
                     /locus_tag="JN692_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7264367.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYSVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5797 a         6037 c         5566 g         5737 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaacccga cagcattagc cttcatcata
       61 tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa
      121 cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt
      181 atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct
      241 gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc
      301 cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac
      361 caattaccac ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca
      421 tgcttgcttt acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg
      481 ccagtaagta ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac
      541 aaagtacggg tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg
      601 ttaaaaactg gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac
      661 gaatctcatc aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta
      721 tacgcgaact gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag
      781 tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa caatgctgta gacttacccg
      841 cattcattgc ggaatagtag aaatatagct gtgccatcag ccacaggccc tcaatgatat
      901 gaaataaagt tggctggagt ttatcataat tcgtcagttt ttcagctcaa cagtatgcac
      961 agaagtgggt atatgcgttc tcccttacga agccttgcat aatccttctg aactatcatt
     1021 acaactgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc acaatcttta
     1081 acctgttgcg caagtaatag ccctctgttg acctccaaga gatagtgcaa tactaagtcc
     1141 atgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat tctcgtaaac
     1201 acaactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca taaaatgtga
     1261 catgaatcag gaagttttaa cctcacgtgc tgcgaaatca tcggtgtaaa tagggctata
     1321 tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac aaaataaaga
     1381 acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg ctctattatt
     1441 acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa gcacttaaaa
     1501 ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt gaaacgctgg
     1561 aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa gaaagcgcgg
     1621 ctgctgctga agttgaagag cgcactcgta agctgcagca atatcgcgaa atgctgatcg
     1681 ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa tctggcacca
     1741 aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac ggcgaaacta
     1801 aaacctggac tggccaaggc cgtactccag ctgtaatcaa aaaagcaatg gatgagcaag
     1861 gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgc acttgcttaa
     1921 aatcccgcca gcggcgggat tttttattgt ccggtttaag acaatttaat aagaataagc
     1981 tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga tacggatgtt
     2041 acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca agggtgttat
     2101 gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg cagtcatggc
     2161 tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata tcaattgcgt
     2221 cggtgagctg aatttcatca ccagctcccg gaggggtttt tgccagcaac ggccaaatat
     2281 ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca tccgctttcg
     2341 gtttttctac cacaccaacc atcggtacgc tttcacccgg cgctaattca acgcctttgc
     2401 aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc tggctatgac
     2461 ccgtttcatc aaagcggcgg atcatctctg ctaggttatc ctgtgacaaa tcggattcat
     2521 attcatccag aataacatca ggcaaaataa cagctaccgg ttcatcaccc actaccgggt
     2581 gagcacacaa taccgcgtgt cccaggcctt tcgccagacc ctgacgaact tgcataatag
     2641 tcacgtgcgg tggacaaata gactgcactt catcaagcag ttgacgtttt acacgttttt
     2701 ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag tttttagatg
     2761 agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc acgacgtatt
     2821 gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc gtcgccggca
     2881 acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc gtattaatgg
     2941 cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt tctgtatcgc
     3001 atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga gatagcataa
     3061 ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat tagcaggtaa
     3121 tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag cgcagtcgac
     3181 caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat tgattcaaat
     3241 aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg tgttcgccgg
     3301 tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt tcgcgatgat
     3361 aatagccaac taatagcgga aactgccccg gcagattggc ctgacgaagt aaatggttta
     3421 cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca agctgttcct
     3481 gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca ttatgtccag
     3541 ctcgggtgac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg gcaatatcaa
     3601 gcaccaggcc gggtttgtcc gcggcaacca attgacggta attaacccgg caatgggaaa
     3661 tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg gcagggttat
     3721 caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc gaattaaaca
     3781 tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct ttaactggtt
     3841 tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc atattttcag
     3901 tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc tccagcagtt
     3961 taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga gtgaaacctc
     4021 ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct cccaatgagg
     4081 aaaaccatga atccagaagc gagcgaaata cctgctcatc ttcaacaatg agaatctgtt
     4141 ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa tagtggcatg
     4201 ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc tggtcaaatg
     4261 ttggtgcgta ccaacggcaa aagttcgtcc attttccttt ccactgccag ctgtccggcc
     4321 gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca aacaggttga
     4381 atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt ctccaacacc
     4441 tggatagacg tggtcataat ctctgttgcc gttggcgctg tcacagcccg acgtgtcgtt
     4501 atgctgccta acctttcttt cagacgcgca tgccacggta gagaatcacc attctcgcta
     4561 ttttcttcac tgacattaaa ggagagcaga tcttgttgca tcaaatgagc atcgtgctgc
     4621 aggtcgaccg ctatcacaat atcagccccc aatgcacgcg taagagaaat aggaattggg
     4681 ttaactactg ccccatcaac cagccagtag ccgttatgtg caacgggtgc catgagtcct
     4741 ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa ccataattca
     4801 cgccccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt ttcgatctct
     4861 gtttccggca ttatttcgcg atattgattg aagacacgct cgccgcgcag taacccaccg
     4921 cgctgccagg agaggtccat caggcgtaaa acgtcccaat aactgaaaga ggttacccaa
     4981 tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga accaattgaa
     5041 catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat aacgccaata
     5101 tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat ctttctcatt
     5161 attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct cggttaacat
     5221 agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt tgtgtctcag
     5281 ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta tgtgtctggt
     5341 gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc ttttgtgatg
     5401 caagacgctg attatttaat aaagacctgg catccctctt gtggagcagc ggcgttacgt
     5461 gccgaattga tgaccggatt tgcgcatact gaatggttgg gattgaccgt atttgaacat
     5521 tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt taccgaaggt
     5581 ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg tcaatggtac
     5641 tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg tggttcaggt
     5701 aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag caaacactct
     5761 caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta ctatttgtcc
     5821 ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg agttaaatat
     5881 cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc gcacggaact
     5941 ggaagggatt tttaatgatt ccaccctgct ggcggatctc gatagcgcat tgccagaagg
     6001 ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg tcactaaaga
     6061 agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggtctgg atgtcgaaat
     6121 cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt ttgatattcc
     6181 gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa agatggcgga
     6241 cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc gggtattaac
     6301 gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt cattcctgcc
     6361 tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga agattattgg
     6421 cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca tgcaggacgt
     6481 tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc gtgacgtcga
     6541 gaaaaacgtc ttaagtcgtg cgctatacaa agtactggcg caacgcgtct ttgtttacgg
     6601 taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt aatccgttac
     6661 ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag cggcgcgtca
     6721 tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag cattaccccg
     6781 tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca tcgacttcga
     6841 aggttcgaat ccttccccca ccaccatcac tttcaaaagc cccggaattc tcaaacgaat
     6901 ctgcaatcaa atattctgcc caagcgggga aggatgagaa gcttcgacca aggttcgact
     6961 tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag cgcgcagcgc
     7021 tgagtaatcc ttcccccacc accatcgctt ccttgaataa tcaaaacctg ttactccgag
     7081 ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc tgtaggtcgg
     7141 ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa ccccgccgaa
     7201 gcagggtcta tgtgaactaa aattcgttta gtgacgagcg cgcaccagct ggtacttacg
     7261 tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg ggaacagcag
     7321 gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac catcgaggtg
     7381 ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca gtttcatcat
     7441 ctcgctaccg tccatatgct gagcggagaa cggaatggtc agcaggccca gcgcgcactg
     7501 gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac gcacgcgtgg
     7561 gctgaacata cgacgtttca gcaaaagcac gccgccaatc agacacagca cgccgctggc
     7621 accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg cttcatacat
     7681 ccagtgcggc gtcaacatac cgaagaagtg accgacaaaa atccccagaa taccgatatg
     7741 gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg acgccgcgcg
     7801 ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga ccgccccggc
     7861 tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg ctgtcctccg
     7921 gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg atgagcagtg
     7981 attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc ttcccagaca
     8041 acatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc cgccactttg
     8101 tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac ggcataacgg
     8161 ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat atctttcaaa
     8221 ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag cggcagatgg
     8281 tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc cagcaggtcc
     8341 accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag caacagtgac
     8401 gtggcgcggc cacggtcgaa cagttcgctg tactgggcct gggcatcgag cggatccatc
     8461 gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga cagatttttc
     8521 gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc atccggatat
     8581 tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt ttgctggtca
     8641 catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct gagccgtggc
     8701 agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc cgtgccagtt
     8761 cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc agataacggt
     8821 acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg gtatcaactt
     8881 taccgtcaac agtttcagca cgtttgtaat gacgcatcgc cagcatacgt ttcagcgcac
     8941 gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt accggaatac
     9001 gcaagctttc gacgtctggc agaattccgt tactgcccag ctcgcccgcg tctgctgcag
     9061 actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga tattctggat
     9121 gcagcggcag cgccagtttc cattccatcg ccattttata aaccggcgac tgctgtgcag
     9181 cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact ttcggatcgt
     9241 tcggatcgag gaacacgtcc agctgacgct ggtagagatc tttctcgttc tcggtgctgg
     9301 ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg atacgaccga
     9361 cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag cagaagatgc
     9421 acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg catccggtga
     9481 tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca tcttcttcac
     9541 gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg cagtgttcgc
     9601 acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc gccttctgga
     9661 tgttgtcgaa gttcttgtct ttcgccagtt tatcaaactc accgcccagg tcatcttccc
     9721 agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa cgcggacggg
     9781 caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgataa tcaaaatcga
     9841 acggctcgta ataatcgtcg atccccggca gatgcgggtt agcaaagatt ttacccagca
     9901 gcatggctcg gttacccatg cgcggttgca gtttgccatt gattttacgg atccagccgc
     9961 ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctggcccggc ttggtttcca
    10021 cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt ttacaggtaa
    10081 ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg acttgtgaac
    10141 gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta accagtcaat
    10201 gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg tgccgtagta
    10261 gttaaagccg tatgccagat ggacatagcc gccgatcata tgcgtcggtt tcggcgtgat
    10321 acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac caggcaggtt
    10381 aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac gctggctgac
    10441 aaccgcacgg gcagtcagtg caccgttgct gttgaagact tcaatccagt cgttatcggc
    10501 gatacccaga tctttggcat cggcttcact caaccagacc accggaccac cgcgacctaa
    10561 agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct ggtgcggcgt
    10621 caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca cttctttcac
    10681 cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat cacgcatcca
    10741 ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga tcagctcgtg
    10801 aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag accaggtcgg
    10861 gctggagata attttgcgcg gctgtgcctg aatatcgcgg aagcggatct tctcgtcttc
    10921 tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg cagcccaggc
    10981 tttcacggct acctgaccgt tggtttccgg tgccagcgtc aggatcatct ctgccgcatc
    11041 aattgcggtg ttcagcatcg gctggccttt tgccggacct tccgctttgg tgtagttgag
    11101 cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac cgccgttacc
    11161 gattttctcc atcagcgggc cgatagaggt aaagcgttcg taagttgccg gataatcgcg
    11221 ctctacgacc ataatgtgcg gtgcagtttt gcctgggatc aggtcgcatt cgcctttttt
    11281 ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcgtgct ggataggcag
    11341 cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga atttcttcgc
    11401 gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga ccgccgcaga
    11461 cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt cgtaccaggt
    11521 cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga agtccagcgt
    11581 aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt cttccggctt
    11641 cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc ccagcaggta
    11701 cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac gccagatgaa
    11761 caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac ggatggaacc
    11821 ctctttcagg gatttcaccg tatagtccac cgaattcatc ccggcttttt ccgcttcgcc
    11881 agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc ccatgcgttc
    11941 cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg ctatcggtga
    12001 cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt tatagaaata
    12061 agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg ccagcggctg
    12121 ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc cgcccccgct
    12181 ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt tcatatcgag
    12241 gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt gcgttttatc
    12301 agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa cgccggtaat
    12361 ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg cacagttaac
    12421 gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa cggtggtcac
    12481 cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt gcagcagcac
    12541 gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt acgggaagcc
    12601 tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg tttcttcgcc
    12661 agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc ggaagccaat
    12721 agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt tccattccgg
    12781 attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac gacctgcagc
    12841 gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt agcgacgcac
    12901 atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca ttacgtggcc
    12961 catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca gatcgcacag
    13021 tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt tgtaacgcac
    13081 ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac cccaggcgat
    13141 gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct cgccccaggt
    13201 ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc aagtaccgcc
    13261 aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg ccggaattgg
    13321 cgagaaacca gcaacacgat ccgggccgta gtttttgatg gtgtaaacgt tagatgcggc
    13381 gatcagttcg ttcacctcct gccaagaaga acgaacaaat ccaccgcgtc cacgcgcctg
    13441 cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct caaccggatc
    13501 gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca tcatcgggta
    13561 tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg ggcagccgcg
    13621 aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct gggtttccca
    13681 ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc agtttacccc
    13741 gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat cctcccagtc
    13801 acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct tctgtttgaa
    13861 gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc ctgtcggccc
    13921 tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa cagcgtggga
    13981 attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa gatacccctt
    14041 taggataatt aacagttaca gcataacatg ctgtaaatta agatgtttta tacctgtagt
    14101 tatttaaact attaagattt ttatgcaaat gggtattaag gagtattccc catgggtaac
    14161 atattaatgg ctcttaatga aatccggcga aataagccat ataacactaa tatgttgtaa
    14221 ctaaatgacg cataaaaaaa gcgcggcatc atgccgcgca aaggataatc aaatattaag
    14281 atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca ggcgatatag
    14341 aaaatcaaaa atactttcat tgcacctact ggcgaacccg ttaatgccag cgagctacca
    14401 aacgctttcg ggataaagaa gccaccaatc gcgccaatcg cagagatgaa acccagcgcc
    14461 gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc ttctgctttc
    14521 acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt ggaaccacta
    14581 cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat gaagcttccg
    14641 ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc catcagaata
    14701 aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc acctgcagaa
    14761 cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg aacatccggg
    14821 aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgatgaagga gccgaaggtt
    14881 gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg caactgctcc
    14941 ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc aatggtgaag
    15001 atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc agtcccatcc
    15061 ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac caccagcgga
    15121 gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag acccagcgcg
    15181 ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga tgcgaagttc
    15241 gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg cgtggaggta
    15301 tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt gctgaacgcc
    15361 gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg taataacgcg
    15421 ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa attaaaaccg
    15481 actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa cgccagcagc
    15541 agacagggaa cggaaatcca caggttgcgg ctggcaatac gttgaccgcg ttgttgccag
    15601 aacgcaggat cttccggtcg ccaatctgtt atgacagctc cagtagccct ttcggaggca
    15661 gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct tagtgtctgg
    15721 agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc attgatattt
    15781 atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt cacggtgctg
    15841 tagaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa ggagtaactc
    15901 tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc agaatatatc
    15961 gtcacattca ttaaggttat tgctcattta aagcctgaag gaaggggttt acatgcttaa
    16021 acgttgtctc tctccgctca ccttggttaa tcaggttgcg cttattgtgt tgctttctac
    16081 tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg ttcagggcag
    16141 cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc tgttggcggc
    16201 agtgccatta agcgagaaag acaagccctt aattaaagag atggaacaaa cggcatttag
    16261 cgccgagttg actcgagcag cagaacgaga cggacaactg gcgcaattac agggtttaca
    16321 agattactgg cgtaatgaac tgatccctgc gctgatgcgt gcacaaaacc gagaaacggt
    16381 gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg gttttgaccg
    16441 caccacggaa atgcgtattg agacagtggt actggtccat cgggtaatgg cggtatttat
    16501 ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac aaccgtggcg
    16561 gcaactgctg gcaatggcga gtgccgtcag ccatcgcgat tttacccaac gcgcaaacat
    16621 cagcgggcgc aacgaaatgg cgatgcttgg aactgcgttg aacaatatgt ctgcagaact
    16681 ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg ggctggagca
    16741 taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt cccgcgcccc
    16801 gctgtgtgaa cgcctgtcac cggtactcaa cggcttacag aatttaaccc tgctacgtga
    16861 tatcgaactg cgggtgtatg acactgatga tgaagagaat catcaggagt ttacctgcca
    16921 gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg tattacccgt
    16981 tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc agtacggtat
    17041 tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac aactggtgga
    17101 taccctggtt gaacaactta ccgccacgct ggcgctggat cgccatcagg aacgtcagca
    17161 acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg attctattgc
    17221 ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg gcgatgcgct
    17281 gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg catcctgggc
    17341 gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg gattacgtcc
    17401 ggcgctggaa gcgagttgcg aagagtacag cgccaaattt ggcttcccgg tgaagctgga
    17461 ttatcaattg ccgcctcgtc tggtgccttc acatcaggca atccacttgt tgcaaattgc
    17521 ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaggtcg tggtgacggt
    17581 ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg gcgtgcctga
    17641 aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg cgcaaagttt
    17701 acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg tggtcacctt
    17761 tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat caggaaccgg
    17821 ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa cagcttatca
    17881 gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag ggtattgaac
    17941 tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc ggcatgaacg
    18001 gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt gtggtattca
    18061 gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg gatggctatc
    18121 tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca gctgctggcg
    18181 aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc gctaaccgtg
    18241 ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt ctcaagctga
    18301 ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc gaaagcacag
    18361 taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc gtggaagcag
    18421 cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag ttcgtcgttt
    18481 gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga gacacgctgc
    18541 ccctggttat cttccaccac cacagacaat cgccagtgat tgcttgcccc ttccccgttc
    18601 tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc gcccggcgtc
    18661 aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta acgactgcgg
    18721 atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg tgtcgccaga
    18781 aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt tcgctgcgga
    18841 ttgtcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc agaaagttgt
    18901 tttttgagcg gtacaccaaa gcggtaatta agattcagcc cgaggttatt ttgattttcg
    18961 ccactttccc cctgtttatg ctgggccgtc acagtgacta atggcacagg ggtgtaattt
    19021 aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa caaatcaaca
    19081 cgatcaccaa aatactgttc taggctgaca ctggtattga ggtgttgata gaacggcatg
    19141 cgcatccgag ctgtcaggtc gtacccgcgc gccatccgtt gttcctgcgt ggctgtctgt
    19201 tcatgccatg cagcaaacgg ctgataaaag tttgccgata atcgcaaata ttcgccccac
    19261 gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc ataaaaagtg
    19321 ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac attgctcacc
    19381 aacccattat cctgctgagt aagaccaagc tggctccagg tgagataacg atcattatct
    19441 tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt atccactttg
    19501 acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg attaacctgt
    19561 tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc gccagtatcc
    19621 agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc aaagtgtttt
    19681 tccccatcat gattttcggg agccatgcct aaatccggca gaccatcatt gttattatca
    19741 aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc gcctgctaca
    19801 agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac gtcggtaact
    19861 tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa cttttgtcgt
    19921 attttctcgc gctttcagga aaatccttaa aaatgtgacc gattggtcag acgctgcaga
    19981 agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa
    20041 atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg
    20101 ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt cctgatgtct
    20161 gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa cattcagcaa
    20221 atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac
    20281 gggcgcggga ttagcacact tcctctgatt gatggggtgg aaatcggtac tcttgtggaa
    20341 ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt aagggggcgt
    20401 ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccttaac acaccatccg
    20461 gataattctc cgccagtagt ttttttaccg ataccagcaa atcattcagc ccatcgtcct
    20521 gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgca ttggttccca
    20581 gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg tcagattcat
    20641 attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt ccatcatcaa
    20701 gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc ttccacaaca
    20761 gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct gtggtgcgtc
    20821 cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc ccggcggtca
    20881 ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcatgat tcggtaaact
    20941 ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt ccctcatcag
    21001 gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac cataagagtg
    21061 attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa tcacgcgtta
    21121 tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg tcttccggac
    21181 atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg gaccacttgc
    21241 aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt tttatgccat
    21301 ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac tttatgcgcg
    21361 gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg atcccatgcg
    21421 ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg ctttacgctt
    21481 tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa agttgaacat
    21541 taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg tcagactgtt
    21601 cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat gccccaatga
    21661 gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca gacactttct
    21721 gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag ggataaccat
    21781 aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc ttattttccc
    21841 ggttctggcg ctggtagtgc tgttcctgtg gggaagtagc cagacactac cagtggtcat
    21901 tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg ttgtccgtca
    21961 tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc ttagcctttc
    22021 agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg acgccgcgcc
    22081 aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg ggctggttgg
    22141 cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat
    22201 caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat ttccaatggc
    22261 tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa tttctgcggc
    22321 aatgtatagc gtatttttgc tgatccagac caaaacgcat caaagtttgt ttgtctacga
    22381 gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg cccatagcag
    22441 cctgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag tcaccaaaat
    22501 gaacgccagc tcgctggaga cattgctcga cagcatgaat gcccctgttg cctttactgg
    22561 cttcctggtg gcactgttga ttctgtcgcc ggaaggttta ggtgcgttaa aagcagtgtt
    22621 gaacaaccag gttcagcgcg cgatgaatct gttctttggt tcagtgttag caacaatttc
    22681 gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat tgcagtttgc
    22741 acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc atatctcctt
    22801 ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta
    22861 tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat cgggagagat
    22921 aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt taatcagaat
    22981 tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa agccggaatg
    23041 tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg tagtatccac
    23101 accaagccgg aattatccgg tgaggcgcaa tgttgcg
//
LOCUS       DADLSE010000052        22409 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLSE010000052 DADLSE010000000
VERSION     DADLSE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.052"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            72..1025
                     /locus_tag="JN692_002051"
                     /pseudo
     CDS             72..1025
                     /locus_tag="JN692_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328142.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            1039..1848
                     /locus_tag="JN692_002052"
     CDS             1039..1848
                     /locus_tag="JN692_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7264368.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQLIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARHHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTRQMIFAEDGDMMLAFFKKYG"
     gene            1942..2199
                     /locus_tag="JN692_002053"
     CDS             1942..2199
                     /locus_tag="JN692_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264369.1"
                     /translation="MQGEKQQPYFFNPGMTVEQLEDWLEQQKLHLSRYNRLVKEKAEL
                     EERLSDISVEIERVSAGGFNGKLSFPWESSSLLRNHQQGSV"
     gene            2279..2665
                     /locus_tag="JN692_002054"
     CDS             2279..2665
                     /locus_tag="JN692_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264370.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLAHLTGYSDKPLDAIGAVIVSALTVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            2652..2933
                     /locus_tag="JN692_002055"
     CDS             2652..2933
                     /locus_tag="JN692_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAY7264371.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            2933..3550
                     /locus_tag="JN692_002056"
     CDS             2933..3550
                     /locus_tag="JN692_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HAY7264372.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAVNDQAMFIA
                     QVGHESAGFTSLVESFNYSVDGLKKTFSKRLTPYQCEMLGRVDGKQVAHQPQIANLVY
                     GDRMGNNSQGDGWKYRGRGLLQITGRENYAKCGAALKLDLISTPELLTQEKHAARSAA
                     WYFTLRGCLMYSGDVVRVTQIINGGQNGLADRNSRYNKARAALLV"
     gene            3547..4077
                     /locus_tag="JN692_002057"
     CDS             3547..4077
                     /locus_tag="JN692_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HAY7264373.1"
                     /translation="MTAVFAFVQARWKTIIVLLMLAGAFLAGNIWSERGWQKKWADHN
                     SMESSQEANAQTAARWIEQGRIIARDEAVKDAQAQAAKSAATAAGLSATVSQLRTEAT
                     KLAARLDAAKHTADLAAAVRSKTTDAGMFADMLGDIAAEAKRYAGIADERYTAGMTCE
                     RIYDSLRESNNAIPHN"
     gene            4144..4419
                     /locus_tag="JN692_002058"
     CDS             4144..4419
                     /locus_tag="JN692_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264374.1"
                     /translation="MSKQGYAVSGHSDGLADILSIVIVLAVEHWIIASLIVGLIMYLV
                     GASINENAQTNAGAIASGIIGVCGQFIFGFGVLASIIGIVKFIWTHT"
     gene            4484..4681
                     /locus_tag="JN692_002059"
     CDS             4484..4681
                     /locus_tag="JN692_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264375.1"
                     /translation="MTVVLTAKQIEDLAAFAKEDGQPQYTITTGTIPEFEADDGEIIP
                     EYTGLIAYSESLEHGVLQLDD"
     gene            4745..5095
                     /locus_tag="JN692_002060"
     CDS             4745..5095
                     /locus_tag="JN692_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7264376.1"
                     /translation="MPPRTPKACRVRGCRNTTTDPSGYCENHKGEGWKSYKPGQSRQQ
                     RGYGTKWEVIRERILKRDKGLCQNHLRQAIVKQASCVDHIKAKAHGGTDEDSNLESLC
                     WSCHAAKTARERLK"
     gene            5222..5716
                     /locus_tag="JN692_002061"
     CDS             5222..5716
                     /locus_tag="JN692_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7264377.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            5713..7446
                     /locus_tag="JN692_002062"
     CDS             5713..7446
                     /locus_tag="JN692_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7264378.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDAVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            7458..7640
                     /locus_tag="JN692_002063"
     CDS             7458..7640
                     /locus_tag="JN692_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264379.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPVGFVVAGALCLFWSWL
                     VARYLDRTQQSVGGGK"
     gene            7640..8881
                     /locus_tag="JN692_002064"
     CDS             7640..8881
                     /locus_tag="JN692_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7264380.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLTESVGMLPCNLYHLNGGLKQRSTGERLHKLISSHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLT
                     QEDIWHVRTLTLDGLVGLNPVAYAREAILLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGPDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKLGIYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            8859..9509
                     /locus_tag="JN692_002065"
     CDS             8859..9509
                     /locus_tag="JN692_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7264381.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSVLNALKNLNF"
     gene            9524..10729
                     /locus_tag="JN692_002066"
     CDS             9524..10729
                     /locus_tag="JN692_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7264382.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDTFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATTVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTCILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            10780..10980
                     /locus_tag="JN692_002067"
     CDS             10780..10980
                     /locus_tag="JN692_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264383.1"
                     /translation="MILKQDLKWSPDGMRVEVIQAGEYDDGALPARVQEIALQAGLAE
                     RGISAKSSKAAKEKKATTSKEG"
     gene            10983..11306
                     /locus_tag="JN692_002068"
     CDS             10983..11306
                     /locus_tag="JN692_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7264384.1"
                     /translation="MLLTMEEIKAQLRLDEDFDTDDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            11303..11713
                     /locus_tag="JN692_002069"
                     /pseudo
     CDS             11303..11713
                     /locus_tag="JN692_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
     gene            11688..12194
                     /locus_tag="JN692_002070"
     CDS             11688..12194
                     /locus_tag="JN692_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264385.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            12191..12751
                     /locus_tag="JN692_002071"
     CDS             12191..12751
                     /locus_tag="JN692_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264386.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            12760..12930
                     /locus_tag="JN692_002072"
     CDS             12760..12930
                     /locus_tag="JN692_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7264387.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            12914..14410
                     /locus_tag="JN692_002073"
     CDS             12914..14410
                     /locus_tag="JN692_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264388.1"
                     /translation="MTISFNTIPSNTLVPLFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATV
                     TLTVNGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPACPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLFHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            14410..14766
                     /locus_tag="JN692_002074"
     CDS             14410..14766
                     /locus_tag="JN692_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264389.1"
                     /translation="MARIGGTCYFKMDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            14766..15035
                     /locus_tag="JN692_002075"
     CDS             14766..15035
                     /locus_tag="JN692_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7264390.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            15177..17012
                     /locus_tag="JN692_002076"
     CDS             15177..17012
                     /locus_tag="JN692_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7264391.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGSPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILILGLMGVGSAISIVSRIMSMTPIGMIATAIAMAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINHWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPVVDLAQSNNASGAIGPNPFMIN
                     KATAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            17073..18401
                     /locus_tag="JN692_002077"
     CDS             17073..18401
                     /locus_tag="JN692_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7264392.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSDVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTCWPSATHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAETWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            18398..19477
                     /locus_tag="JN692_002078"
     CDS             18398..19477
                     /locus_tag="JN692_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HAY7264393.1"
                     /translation="MNDNVTLRVNGREWNGWTLVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATTVRYDARSVITGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            19477..20025
                     /locus_tag="JN692_002079"
     CDS             19477..20025
                     /locus_tag="JN692_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HAY7264394.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     IEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMGA"
     gene            20025..20450
                     /locus_tag="JN692_002080"
     CDS             20025..20450
                     /locus_tag="JN692_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7264395.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            20437..21495
                     /locus_tag="JN692_002081"
     CDS             20437..21495
                     /locus_tag="JN692_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7264396.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFVRLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRLEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYLQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            21486..22070
                     /locus_tag="JN692_002082"
     CDS             21486..22070
                     /locus_tag="JN692_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY7264397.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAQSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            22074..>22409
                     /locus_tag="JN692_002083"
     CDS             22074..>22409
                     /locus_tag="JN692_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264398.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTLATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGAE"
BASE COUNT         5258 a         5507 c         6675 g         4969 t
ORIGIN      
        1 gttaaagctg ctcgataccg ttcattgagg tgaccgtgag agcattactg acccctgaaa
       61 ttgccccgcg tatggggatc gtattgttca gacccggttc agagctgatg cccctgttta
      121 tgcaggggcg tgtcctgctg gagcctgagc cggaacgtta ttcatctttt gccagtggtg
      181 ccgttccggc ggcatcacaa ccgctggcgg atgatcctgc tgttcgggcc gtgttccgca
      241 atgaggcagt gatccgtcgt gctggtggcg tggaatgtct tgaaagctgg ttacttcgtg
      301 aaaaaggctg ctagtggcct cattccgact ggcacagcga gaacatgaca acaatgcgac
      361 acgctccggg cgcaatccgt ctgtgctggc actgcgataa ccagctgcgc gatcagttca
      421 cggaacggct ggaatcaatg gcaacggata actgtgcccg ctgggtgttg tctgttgtgc
      481 gtcgggatct cggttttgat gacagtcacg ttgtgacaat gccggaactg tgctggtggc
      541 tggttcgtaa tgatctggcg gatgccttac cggaaagcgc agcccgtaag gcactgagat
      601 taccgaatcc tgttgtgccg tctgtcaccc gggaaagtga ccttgtgcct tcggttcctg
      661 ccaccagcat catccaggat aaggcgaaaa aggtgctggc gctgaaagtg gatccggagt
      721 cgccggagtc ttttatgtta cgcccaaaac gtcgccgctg ggttaatgaa aagtacacgc
      781 gctgggttaa gacacagccg tgcgcatgtt gtggaaagcc agccgacgat cctcatcacc
      841 tgattggtca tggtcagggt ggaatgggta caaaagcgca tgacctcttt gtgttgcctt
      901 tgtgcagaaa gcatcacgac gagctgcatg cggataccgt ggcatttgaa gagaagtatg
      961 gctccctgct ggagctgata tttcgtttta tcgatcgtgc gctggcaata ggcgtactgg
     1021 cgtaagtgga gaacgagcat gaaccttgaa gccttaccaa aatattactc cccaaaatct
     1081 ccaaaactga gcgatgacgc accggcgaca ggctcgggtg gtttaacgat tacggatgtg
     1141 atggctgcgc aggggatggt gcagtcgaaa gcaccgcttg gatttgcctt attcctggca
     1201 aaagttggtg ttcaggatcc tcagtttgcg attgaaggtc tgctcaatta cgcgatggca
     1261 ctggataatc cgacattgaa taaattgagt gaagaaaccc ggttacagct catcccttac
     1321 cttgtgaatt ttgcctttgc tgattattcc aggtctgcgg caagtaaggc tcgctgtgag
     1381 cattgtgctg gtactggatt tcataatgta ttgcgcgaag tggtgaaaca ctccagaagc
     1441 ggtgaatctg ttatcaagga ggagtgggtg aaggaactat gtcagcattg tcatggtaag
     1501 ggagaagtca gcacagcgtg cagagggtgt aagggtaaag gtattgtcct ggatgaaaaa
     1561 aggacccggc ttcatggcac gcctgtttat aagatttgtg ggcgttgcaa tggaaaccgg
     1621 tttagccgtt tgccaaccac actggcgcgg catcatgtcc agaagctggt accggacctg
     1681 acggattatc agtggtacaa aggatatgca gatgtcattg ataaactggt tacaaagtgc
     1741 tggcaggaag aagcatatgc agagacacaa ttgagaaaag tgacaagaca aatgattttc
     1801 gccgaagatg gcgacatgat gcttgcattt ttcaaaaaat atggttagga ttctcctaac
     1861 gatgggcttt gtatgtctgc cgttaacgaa atcataacaa acctcgcttc ggcggggttt
     1921 ttgcttttct ggaggtcaat aatgcagggc gaaaagcagc agccatattt ttttaaccct
     1981 ggtatgactg ttgaacagct tgaagactgg ctggagcagc aaaagcttca tctaagccgc
     2041 tataaccgtc tggtaaaaga aaaagcagag cttgaagaac ggctcagtga tatttctgtg
     2101 gaaattgaac gagtgtctgc tggtggtttt aacggaaagt tgagtttccc ctgggagtca
     2161 agttcgcttc tgagaaatca tcaacagggt agtgtttgac tgaaataata aatagactgt
     2221 cattaagatc ccttcccctc atatctgaga ggaccaacag caattaagag ggggctaaat
     2281 gtccgatccg atttccggta ctgggctggc tggtggtgcc ctgacgggtg ccagtgttta
     2341 tggactgctg accggaactg attacggcgt tgtatttggc gcatttgcag gggctgtatt
     2401 ctacatagca acagcagcag atctgagtgc atcgcgccga ctggcatatt ttatcgtgtc
     2461 atatattgcc gggatccttt gctctgggtt ggttggctcc aagctggcgc acttgaccgg
     2521 atacagtgat aaacctctgg atgctattgg tgccgtaatc gtctctgctt taaccgttaa
     2581 aatcctgacg ttcctgaata atcaggatat cggctcgctg gtggcgctca taacgcgccg
     2641 gggaggttca ggtggagcta aatgacccga cagcaactat aaatgcgctg ttatgtgctt
     2701 gtgttgttat tactctgatg ttttatcgtc gtggtgattc gcggcatcgt ccttgggttt
     2761 cacgtttagc ctggctgatt actgttacat acagtgctgt tccgttggcc tatctctgtg
     2821 ggatttatcc ccattcctca tggcccatta tcgtggcgaa cactattttt ctttccgtgc
     2881 tggtggccgt cagaggcaac gttgcacgtc tggttgatca tctgaggcac taatgaacca
     2941 acaattattt caaaaggcgg ctggtattag cgccgggctg gctgcgcgct ggtttccgca
     3001 cattgatgct gcaatgaaag agtttggaat cacagcagtt aacgatcagg ccatgttcat
     3061 tgcacaagtt gggcatgaat ctgctggttt tacctcgctg gtggaaagct tcaactactc
     3121 ggtagacggg ctgaagaaaa cctttagtaa acgcctgacg ccttatcagt gtgaaatgct
     3181 ggggcgtgtc gatggtaagc aggtagccca ccagccgcaa atagccaatc tggtttatgg
     3241 tgaccgcatg gggaataaca gccagggtga cggctggaaa tatcgcggtc gtggcctgct
     3301 tcaaatcacc ggccgcgaga actacgccaa atgcggtgcg gcgctgaagt tggatctgat
     3361 cagcacacca gagttgctga cacaggagaa acatgcagcc cgttctgctg catggtattt
     3421 cacattacgt ggttgtctga tgtattcagg tgatgttgtc cgtgtaacgc agatcatcaa
     3481 cggtggccag aatggactgg ctgacagaaa tagtcgttat aacaaagcgc gggcggcgtt
     3541 gctggtatga cagcggtctt tgcttttgtt caggcgcggt ggaaaacaat cattgttttg
     3601 ctgatgttgg ctggtgcatt tcttgccggg aacatctgga gtgagcgggg ctggcaaaag
     3661 aagtgggctg accacaatag catggaatct tcacaggaag cgaacgcgca gactgccgca
     3721 cgctggattg aacaagggcg cataattgcc cgtgatgagg ctgtaaaaga tgcacaagca
     3781 caagccgcta aatctgctgc cactgctgct ggcctgtctg ccactgttag ccagttgcgt
     3841 accgaagcaa caaagcttgc cgcacgcctg gacgccgcaa agcacaccgc tgatcttgcc
     3901 gctgccgtcc gaagcaaaac aaccgacgcc ggaatgttcg ccgacatgct cggagatatt
     3961 gcagcagaag ctaaacgtta tgctggaatc gctgacgaac gctacaccgc cgggatgacg
     4021 tgtgaacgta tttacgactc gttgagagag tcaaataacg ctatccccca caattgattt
     4081 tttatcatat tcgaacgact atgaagggtg taaacaatta taatccagtg atgaattttt
     4141 caaatgtcaa agcaaggata tgcagtgagt ggtcatagtg atggtttagc agatattttg
     4201 agcattgtaa ttgtattagc agtggaacat tggattatcg catctctcat agttggtttg
     4261 attatgtatc tagtgggggc gagcataaac gaaaatgctc agacaaatgc cggtgccatt
     4321 gcatcgggaa tcataggggt atgtggtcaa tttatttttg gttttggcgt tctggctagc
     4381 attattggga ttgttaaatt catttggacc catacctgac agcatcactt tagtcaatca
     4441 tagcctcgca atagcggggc tttttaacaa ctgaggtatg agcatgacag tagttcttac
     4501 agctaagcag attgaagacc tagcagcatt cgcgaaagaa gatggtcagc cccaatacac
     4561 catcaccact ggtacaatcc cggaattcga agcggatgat ggcgagatta tccctgaata
     4621 cacagggctg atcgcttact cagagtcact tgagcacggt gtattgcaac tcgacgatta
     4681 gtggcattca gcaggcattc attaagtgtc tgcgataatg ctatacgttt tttggggagt
     4741 gaatatgcca ccgcgcacac caaaggcttg ccgtgttcgc ggctgtcgca acaccacaac
     4801 agacccatct ggctactgtg aaaatcacaa aggtgaaggc tggaagtcct acaagcctgg
     4861 tcaatcacgg cagcagcgag gatacggaac aaagtgggaa gtcatccggg agcggatact
     4921 taagcgcgac aaagggctgt gtcagaacca tctacggcag gcgatagtga agcaggcgtc
     4981 ctgcgtggac cacatcaagg cgaaggccca cggcggtact gatgaagaca gcaaccttga
     5041 aagcctgtgc tggtcgtgtc acgcagcgaa gaccgcgcgt gagcggctca agtgataatt
     5101 aatgtcatca tcagactggg gagggggagg tcaaatctct gtgaccgcgc gccttccgga
     5161 ctgcccgccc catcgttttt ttatacccgc gaaaaatgaa atttaaccag gagtgccgca
     5221 tatggctgga acggcggggc gttccgggcg tcgccccaag ccaacggcgc gcaaggcgct
     5281 ggccggaaac cccggcaagc gagccctgaa taaagatgaa cctgttttta cgcccatcaa
     5341 aggtgttgag ccaccggagt ggttcgctga agaagatctc cctctcgcta cgatcatgtg
     5401 gcaactgaca actaaagaac tctgcggtca gggcctgctg tgcgtgactg acctcgcggt
     5461 gcttgagcgg tggtgcgtgg cctacgagtt ctggcgacgt gccgtgaaaa atattgccag
     5521 acagggcaac accatcaccg gtgcaatggg cggtatggtc aaaaatccgg agctgaccgc
     5581 caaaaaagaa caggagtccg agatgagcag tacgggggca atgctcggac tcgaccccag
     5641 cagccgccag cgtctgattg gcctggcggg gcagaagaaa gccactaacc cgtttctgaa
     5701 aatcatcgaa tcatgagccg gaaatcttac cccaacgtaa atgctgccaa tcagtatgcc
     5761 cgtgatgtcg tgcgcggaaa gattgtggcc tgccagtttg tgattcaggc ctgccagcgc
     5821 catcttgatg acctgatggc ggaaaaaagt aagtcgtttc gttaccgctt cgacaaggac
     5881 ctggctgaac gggccgcgaa atttattcag ctgttgccac acaccaaggg tgagtgggca
     5941 ttcaaacgga tgcccatcac gctggagccg tggcagctct ttgtgatctg ctgcgcgttt
     6001 ggctgggtca ataaaggctc ccggctgcgc cgcttccgtg aggtgtatac cgaaatcccc
     6061 cgtaagaacg gcaaatcggc aatctctgcc ggtgtcgccc tgtattgttt tgcctgtgat
     6121 aacgagttcg gcgcggaagt gtattccggt gccacgacgg agaagcaggc atgggaagtc
     6181 tttcgtccgg cacgactgat gtgtaaacgc acccccatgc tgacggaagc gttcgggatt
     6241 gaggttaacg cctcaaacat gaatcgtccg gaggatggcg cgcggtttga accgctgatc
     6301 ggtaaccccg gtgatggttc atcaccccac tgtgcggtgg tggatgaata tcacgagcac
     6361 gccaccgatg cgctttacac cacgatgctt accgggatgg gggcgcgacg tcagccactg
     6421 atgtgggcta tcaccaccgc cgggtacaac attgaggggc cgtgctacga caagcggcgg
     6481 gaagtcatcg agatgctcaa cggctcggtg cctaacgatg aactgttcgg gatcatctat
     6541 accgttgatg aaggtgacga ctggaccgac ccgcaggtgc tggaaaaagc caatccaaat
     6601 attggcgtgt cggtttatcg cgaatttttg ttaagtcagc agcagcgtgc gaaaaataac
     6661 gcccgtctgg caaacgtctt taaaacaaaa cacctcaata tctgggtgtc ggcgcgttcg
     6721 gcgtatttca acctggtgag ctggcagagc tgcgaggata aatcactgac ccttgagcag
     6781 ttcgaggggc agccgtgcat tctggccttt gacctggcgc gtaagctgga tatgaacagc
     6841 atggcgcgac tttatacccg cgagattgac ggtaaaacgc attactacag tgtggccccg
     6901 cgtttctggg taccgtatga cgcggtgtat agcgtcgaga aaaatgaaga tcgccggaca
     6961 gccgaacgct ttcagaaatg ggtggaaatg ggcgtcctga ccgttaccga tggtgcagag
     7021 gtggattatc gctacatcct cgaagaggcc aaagcggcga acaaaatcag cccggtcagc
     7081 gagtcaccca tcgacccctt cggggcgacc gggctgtcac atgaccttgc tgatgaagac
     7141 ctgaaccccg tcaccatcat tcagaactac accaacatgt ccgacccgat gaaagagctg
     7201 gaagcggcaa ttgaatcggg gcgctttcat catgatggca atcccatcat gacctggtgt
     7261 atcggcaacg tggtcggaaa aaccattccg ggtaacgatg atgtggtgaa gcccgtcaaa
     7321 gagcaggcgg aaaacaaaat cgatggtgca gttgcgctga ttatggcggt tggcagagcc
     7381 atgctgtacg agaaagaaga cacgctgtct gaccacattg agtcctacgg gatccgctcg
     7441 ctttaactga ggtaattatg atcatgctga ttctcgcgcc tctggtaggc gtgctggggg
     7501 cgcttttgct ggcgtatggt gcctggctga tttatccccc ggtgggtttt gttgttgccg
     7561 gggcgttgtg cctgttctgg tcgtggctgg tggcgcgata tctcgaccgt acacagcagt
     7621 ctgtcggcgg aggtaaatag tgttcttttc gggattattt caacgaaaaa gtgacgcacc
     7681 ggtgaccacg ccagcagagc tggcggatgc tatcgggttg tcctacgaca cctataccgg
     7741 aaagcagatc agcagtcagc gggccatgcg actgacggcg gttttttcct gtgttagggt
     7801 gctgacggag tcggtcggga tgttgccctg caacctgtat cacctgaatg gcggtctgaa
     7861 gcagagatcc actggcgaac gtctgcataa gctgatctcc tcgcatccta atggctatat
     7921 gacgccgcag gagttctggg agctggtggt cacctgtctg tgcctgcggg gcaactttta
     7981 cgcctacaaa gtgaaagcat ttggtgaagt ggctgaactg ctgcccgtcg atcccggctg
     8041 tgtggtaccg aagcttaaca gtagctggga gccggtctat caggtcacat tcccggacgg
     8101 ctcaacggat gtgctgaccc aggaggatat ctggcatgtg cgcacgctga cgctggacgg
     8161 tctggtggga ctgaatcccg tcgcctatgc ccgcgaggca atattgctgg cagcagcgac
     8221 tgaagaacac ggtgccagac tgttcagcaa tggcgcggtg acgtccggtg tgttgcgtac
     8281 agaacagacg ctgtcggatc aggcttatga gcgcctgaag aaagattttg aggagcgtca
     8341 caccgggctt ggcaatgctc accgcccgat gatccttgag atggggccgg actggaagtc
     8401 gatggcattg aacgccgagg acagccagtt cctggaaacc cgcaagtttc agcttgaaga
     8461 aatctgtcgt ctgttccggg tgccattgca tatggtgcag aacaccgatc gcgccacctt
     8521 caacaatatc gaagagctgg ggctgggatt tatcaactat tcactggtgc cgtatctgac
     8581 ccgcattgag cagcggatca acaccggact ggtacgaaaa agtaagctgg gcatttatta
     8641 cgccaaattt aacgccgggg cgttactgcg cggggatatg aagtcccgtt ttgaagccta
     8701 cgccaccggg atcaactggg gaatttactc tcccaatgac tgccgcgacc tggaagatat
     8761 gaatccgcgt cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc
     8821 cgatggcagt aaagccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg
     8881 atgtaccgct gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg
     8941 tctttggtgt aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc
     9001 tgcgggcgtg gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg
     9061 aacccatcgg tgtttatacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac
     9121 ggttgctcat tgatgatgat cccctcgcaa aacgcgcaca tgcacacatg aaggccggtt
     9181 cgttaaccgg cctttctatt gggtacgtcc tgaaagactg ggaatacgac cggagtaaag
     9241 aagcctttct gctgaaagaa atcgacctct gggaagtcag tctggtgacg ttcccgtcta
     9301 acgacgaggc gcggatcagc gacgtcaaga acgcgctggc ccgcggtgaa atccccgaac
     9361 agaaaaaaat cgaaagagtc ctgcgtgatg tcggactctc ccgtacccag gccaaagcat
     9421 tcatggccgg gggctatggc gcactgtccc tgcgcgacgc tgaggatgtg ggctctgtac
     9481 tgaatgcact gaaaaatctg aacttctaat caggagaaat acgatggcgg ttgatattaa
     9541 agatgtggaa caggtcgcgc aggagctgca gcagaagttt gacgacttca aagcaaagaa
     9601 cgacaagcgc gtggatgcga ttgagcagga aaaaggcaaa cttgccgggc aggtggaaac
     9661 cctgaacggg aaactcagcg agctggaaaa cctcaaaagc gatcttgaaa aagagctgct
     9721 tgagctgaaa cgtccggcag gtggtgcgca aaataaactg gccaccgagc ataaagaagc
     9781 gtttgtgggc ttcctgcgta aaggccgtga agatggtctg cgcgatctgg agcgcaaggc
     9841 attacaggtg ggcaccgatg aagacggcgg ctatgccgtg ccggaagcac tggatcgcaa
     9901 cattctcacc ctgctgaaag atgaagtggt gatgcgccag gaagccacgg tgatcaccgt
     9961 tggcggttcc gactacaaaa aactggtgaa tctgggcggc acggcttccg gatgggttgg
    10021 cgagactgac gcgcgctccc agactgccac ctcaaaactg ggactgattg aacctttcat
    10081 gggggaaatc tacggtaacc cgcaggccac tcagaaaatg ctggatgata ccttcttcaa
    10141 cgtggaggcc tggatcaaca gcgagctggc gaccgaattt gccgaacagg aagaaattgc
    10201 ctttaccacc ggtgatggca ccaagaagcc aaaagggttc ctggcgtatg aatccaccga
    10261 tgaaaccgat aaggttcgtg cgttcggtaa acttcagcat attgtatccg gtgaagcgac
    10321 gacggtgacc gcagacgcca ttatcaaact gatttacacg ctgcgtaagg cacaccgcac
    10381 tggcgcgaag ttcatgatga acaacaacag cctgtttgcc atccgtctgc tgaaagacac
    10441 cgagggtaac tatctgtggc gtccggggct ggagctgggg caaccgtcct ctctggcggg
    10501 ttacggtatc gctgaaaacg aacagatgcc ggatatcgcc gctgatgcga aagctattgc
    10561 atttggtaac ttcaagcggg gttacaccat cgttgaccgt atcggtacct gcattctgcg
    10621 tgatccgtac accaataaac cgtttgtcgg tttttataca accaagcgca ccggcggcat
    10681 gctggtcgat tcgcaggcca tcaaactgct gaagattgca gcggcgtaat cactcagggg
    10741 cgcggaaccg cgccccctgt tctgacaggt gaaagaatca tgatcctgaa acaagatctg
    10801 aaatggtcac cggacggtat gcgtgttgag gtcattcagg ccggtgagta tgacgacggg
    10861 gcgcttcctg cccgggtgca ggagattgca cttcaggccg ggttagcaga gcgcggaatc
    10921 agtgcaaaaa gcagtaaagc ggcaaaagag aaaaaagcca cgaccagtaa agagggctga
    10981 gtatgcttct gacaatggaa gagattaaag cccaactccg gctggatgag gatttcgata
    11041 ctgatgaccg ccatctgcaa ctgctggcat gtgcggcaca aaagcggacg gaaacgtatc
    11101 tgaaccggaa gctctatgca ccggatgaaa ccattccgga cagcgatccg gacgggctgc
    11161 acctgccgga tgatattcgt ctggggatgc tgatgcttat cagccatttt tacgaaaacc
    11221 gctcgtcggt tacggaagtg gagaaactcg acatgccgca gagttttggc tggcttgtcg
    11281 gcccgtacag gtactttccg caatgaaaat tcgtcaggcg cagaccagcg caacctacat
    11341 tctgccggac cccggtgaac tgaataaacg cgtcctgatc cgcctgcggg tggatatgcc
    11401 cgcggataac tttggcgtgg agcctcaata cccggttacg ttccggacat gggcgaaggt
    11461 tatccagacc agtgccacca cctggcagta aaccgcgcag accggggacg ccatcaccca
    11521 ttacatcacc attcgttacc gccgggggat caccgctgat tatgaggtgg tctgcggtga
    11581 cagtgtgtac cgggtgaaac gtcagcgcga tctgaacggg gcgcggcgct ttctgctgct
    11641 ggagtgtacg gagctgggcg aatgtaggca gagtcacgga ggcaacaatg acgacttcct
    11701 ttttgcacgt tgattttcag cagcccgcgg agatgcgctt taaccgcgcc cgtgtccggc
    11761 gggcgtttgt cacgattggt cagcgtcata tgcgtgatgc ccgtcggctg gtgatgcgcc
    11821 gtgcgcggtc ggcaccgggt gaaaaccccg gttatcagac cggacgcctg gctcgttcga
    11881 ttggttacat ggtacccaga gccagtaaac atcgccctgg ttttatggca cgtatagccc
    11941 ctaaccagcg taatggagag ggaaaccgcc gtatcaccgg tgatttttat ccggcttttt
    12001 tgttctatgg cgtgaggcga ggggcaaagc gtcgtcgcag ccatcatcgt ggtgcatccg
    12061 gtggcagcgg ctggcgactg gctccacgta ataacttcat ggtggaaacg cttgaaaaga
    12121 accgcagctg gacacgctat tttctggcgc gggaattgcg taaatcactg aagccggagc
    12181 gacgacacag atgaaactga cgcctgttat tgctgcactg cgtgcccgct gtccgtattt
    12241 tgaaaaccgg gttgcaggcg cggcccagtt caaaaatctg ccggaggtcg gaaagctgaa
    12301 actcccggcg gcatatgttg taccgggtga tgattctccg ggagaaaaca aaagccagac
    12361 cgactactgg caggagctga aagagggttt ctccgtggtt gtcatactga gtaacgggcg
    12421 tgatgagcgc ggtcagtttg cctcgtatga tgtggtggac gatgtccggc agatgctctt
    12481 taaggctctg ctgggctgga acccggaggc gtgcggtaac ccgattacct atgacggcgg
    12541 cacgctgctg gatctgaatc gtcatgagct gatttatcag ttcgattttt cggtcatcag
    12601 cgagctgact gaagacgata cccgccagca ggatgatctg aacagtctgg atgaactgca
    12661 aacgctggcg attgatgttg attatctcga gcccggtaac gggcctgacg gcgatatcga
    12721 acatcacacc gaaataaccc ttccttcctg aggatcctca tgtttgtcaa acctgttaaa
    12781 gggcggtcag ttcctgaccc tgcccgcggc gaccttttgc ccgccgaagg gcgaaatgtt
    12841 gacgagaaca actactggct gcgccgtgaa gcagcgggtg atatccggcg cgtgaataaa
    12901 aaggtgaata ccgatgacga taagctttaa caccattccg tcgaatacgc tggttccgtt
    12961 gttttatgcg gaaatggata actcggcggc gaatactgca caggacagcg gagcatcgct
    13021 gctgattggt catgccaata acggtgcaga gattgttgcc aacagtctgg tactaatgcc
    13081 gtcggcagac tatgcacgcc agatttgtgg tgcgggaagt cagctggcgc gtatggtcga
    13141 ggcttatcgc cagaccgacc cgtttggtga gctgtatgtg attgccgttc cggaagccac
    13201 aggcgcggcg gcaacggtta cgctgacggt gaacggggcg gcaaccgaaa ccggcacggt
    13261 gaatgtttat gtgggacgta cccgcgtgca ggcaccggtg accaacggcg ataacgtcac
    13321 gacgattgcc agcagtatcc aggatgccat caatgccgtt ccggccctgc cgtttacggc
    13381 ctcatcttcg gcaggcgtgg tcacactgac cgcgcgtcat aaggggcttt gcgggaatga
    13441 aattcctgtc agcctcaatt actacggctt tggtgggggc gaagtgctgc cagcgggcgt
    13501 acagattgcc gtggcgacgg ggaccgccgg aacgggcgct ccggttctca ccggtgcggt
    13561 ggctgcaatg gcggatgagc cgtttgatta tatcggtctg ccgttcaacg acacggcctc
    13621 cgttaacacg ctggtgaccg agatgaacga taccagcggt cgctggagct atgcgcgtca
    13681 gctgtatggt catgtgtata cggcaaagat cggcacgctg tcagaactgg tgaccgcagg
    13741 tgaccagttt aaccagcagc acatcaccct ggcggggtac gaaaaagaga cccagacgcc
    13801 tgccgacgag ctggcggcaa gccgtaccgc ccgcgcagcg gtgtttatcc gcaacgatcc
    13861 ggcatgtccc acgcagaccg gtgagctggt gggtatgctg cctgcgccga aggggaaacg
    13921 gttcacgatg accgaacagc agaccctgct gtttcatggc gtggcaacgg cgtatgtcga
    13981 aagcggggtg ctgcgcattc agcgtgatgt caccacgtac aggaaaaatg cttacggggt
    14041 tgcggataac agctacctcg acagcgagac gctgcatacc agtgcgtatg ttctgcgcaa
    14101 actgaaatcc gtcattacca gtaagtacgg gcgtcacaag cttgccagcg acggtacccg
    14161 ctttggtccc ggtcaggcga ttgtcacccc ggcggtgatc aaaggggaac tgctggcaac
    14221 ctaccgtcag cttgagcgtg cggggatcgt ggaaaactac gaactgttta agcagtacct
    14281 ggttgtggag cgtgatgcca gcgatccgaa ccgcctgaac acgctgttcc cgcctgacta
    14341 tgttaaccag ttgcgtgtct ttgccgtggt taaccagttc cgtcttcagt attcagagga
    14401 gtctgcataa tggcccgtat cgggggaacc tgttatttca aaatggacgg tcagcagcta
    14461 tcgctgaccg gcggcattga ggtgcccatg aacaggacgg tcaatgatga catcatcggc
    14521 ctggacggtt cagtggaccg caaggaaact caccgtgcgc cttatgtcaa agggaccttc
    14581 aaggtgccga agaattttcc ggtgagcaaa atcacctcgt ctgatgagat gaccatcact
    14641 gccgagctgg cgaacggtca ggtctatgta ctgtcgtctg cctggctgca cggcgaagcg
    14701 aaccataatg ccgaagaagg cacggtcgat cttgagttcc acggtgaaga aggggattac
    14761 cagtaatgaa agagcttgag ttaaagaaac cgattaccgc tcatggcgag acactctccg
    14821 tactggagtt tgatgagccc accgggaagg atgtccgcga gctggggtat ccctaccaga
    14881 tgaatcagga tgagtccgtc agacttctgg cgcatgtggt gtcgaaatat attgtgcggc
    14941 tggcgaaagt gccgcaaagc tctgtcgacc agatgtctcc ggcagacctg aatgcagcgg
    15001 cgtggcttgt ggctggtttt ttcctccagg cctgacggct gaatacctta ctgatcgctt
    15061 ctttgattgc gccagctact ggcgcattaa tcctttcgaa ttgctgaata tgccgatcag
    15121 tgaaattccc ttactggtca gtcaggcaaa caggatagag caggagaaac gcacacatgg
    15181 ctgaatttga gcttaaggcg ttgatcaccg gtgtcgacag gctttctccc gcgctgtcga
    15241 aaatgcaaaa gaaaatccgg ggatttaaac gccaggcgga agaagcgtca cagggtgggc
    15301 tggcgcttgg tggcggactg gcagcgggtc tgacgctttc cctgaaatct tatgccgatc
    15361 aggaaaacgc cgccaccggg ctgaaagtcg ccatgatgga tgcgaacggc gaggttggaa
    15421 agagctttca ggacatcaat aaactggcta ttggcctggg taaccagcta cccggtacaa
    15481 cggctgattt ccagaacatg atgcagatgc tggtgcgtca ggggagcccg gcagaaaaca
    15541 ttcttggcgg tgtgggtaaa gcgacagctt atcttgcggt acaactgaaa aaaacaccgg
    15601 aagcggctgc tgagtttgct gcaaagatgc aggatgctac cggaacggcc tcagaagaca
    15661 tgatggggct gttcgacaca attcagaagg cgttttatct gggcgttgac gacaccaaca
    15721 tgctgtcatt cttcactaaa accagctctg ttctgaagat ggtgaataag gacggtcttc
    15781 aggctgcaca gagtcttgcc cccatcagtg tcatgatgga tcagatgggg atgaacgggg
    15841 agtcggcagg taatgccctg cgaaaagtta tccagtccgg attaagcgtt aagaaaatca
    15901 gggacgtcaa taaaatcatg gcccgccaga aactcggggt acagctcgat tttactgatg
    15961 gcaaagggag ttttggcggc cttgataaca tgttcaggca actggcaaag ctgcgaaaac
    16021 tgaccgacgt taagcgaacc ggtgtactta aggcaatatt tggtgatgat gccgaaaccc
    16081 ttcaggtggt caatgcgctg atcgataaag gaaaggatgg ctacgatcag atccagcaga
    16141 agatgaataa acaggccagc ctgaataaac gtgttcaggc tcagcttggt acgctgtcca
    16201 acctgtggga ggcaatgacg gggaccgcaa ctaacggcct tgcggctatt ggcggcgcat
    16261 tttctggtga cgctaagaac atcacgcagt ggctggggga gttgggggag aaattcacga
    16321 agtttgcgga tgaaaatccc cgggttattc gcggcgtcgt cgggcttgct gccggtcttg
    16381 cgattctgat actgggattg atgggcgtgg gcagtgccat cagcattgtc agcaggatca
    16441 tgtcgatgac gccgattggc atgattgcga cggcgatagc catggctgcg ggattaatta
    16501 tcactaactg ggatgttgtc ggaccttatt tcaagaagct ctgggaaacc attggtcctt
    16561 attttgaggc tggctgggaa ctccttaaga aagtttttgc ctggtcgccg ctggggatgg
    16621 tgatcaatca ctgggggccg gttgttaagt ggtttcagga tatgtgggac aagctgaagc
    16681 caattattga gtggtttacc gacagttccg gagacacggt cgatgccatt aactctgcgc
    16741 agtggggcgc gggtgcttat gatgcttatg ggacgggaat accggcacgg ggatacacac
    16801 cttatccggt ggtggatctg gctcagtcaa acaacgcctc cggtgccata ggcccgaatc
    16861 ccttcatgat taataaagct actgcgccaa aagttgatgg tgagatcaag gtctcttttg
    16921 tgaattcgcc tccgggtatg cgggttatgg aaacgcgatc cagcggtttt gatgtcagcc
    16981 atgatgttgg ctatacgcgc tttggcaggt aatgaaaaat taatctgtta atgagtccca
    17041 caccggtggg attttttatg tacggagttt atatgacgtg gaaagacaga cttcaggacg
    17101 cgtcatttcg cggtgtgccg tttaaggttg aagaagaaag tgcgggaacc ggtcgtcgtg
    17161 tggaaacgca cgaatacccg aaccgcgaca aaccctatac cgaagacctg gggaaaatca
    17221 ctttccgccc gtccatcacg gcttatgtgg tgggcgatga ctgctttgac cagcgcgatc
    17281 gcctgattga cgcgctgaat aaacccggtc ccggcacgct tgtccatccg acatacggcg
    17341 agctgaaagt ctgtgttgac ggagaagttc gggtcagcac atcgaagagt gaagggcgta
    17401 ttgtccgctt tgacctgaag tttgtcgaag cgggagaact ctcttacccc acatcaggtg
    17461 cggcgacggc gcagacgctg atgtcatcct gttctgcact ggatgactgc atcagtgaca
    17521 gcttcagcgg tttcagtatc gatggtgtgg cggatttcgt gcagaacgac gttatcggta
    17581 atgccagcat aatgctgggg tatgtttctg atgcgatgaa agtggtggat tctgccgtat
    17641 cggatgccgc caggctgttg cagggggata tctcggtact tctgccgccg ccatcgtcag
    17701 gcaaaaattt cgttgagcag gtgcagaaaa tgtggcgtac cgggaaacgc ctttatggta
    17761 acgccagcga cctggtcacc atgatcaaaa cgctttccga tgtcagcctc ggcagcgatc
    17821 tgcaaccgcg cggcgtctgg aaaacggaca gtaaaaccac cgccacggcg acgcagcagc
    17881 gtaacgtggt tgccagcacc cttcgtacga ccgcaatcag cgaagcggcg tatgccgtta
    17941 cccgattgcc tgcgccaaca acttccgcgg tgatgcagaa tgccgcagtg gggcaggcaa
    18001 caacacccgc gcagagcact tgctggcctt ccgccacgca tccggcactg aacaatgcac
    18061 cggcggtgaa aaacacggtt gacctgccga cgtgggaaga actgactgac attcgcgaca
    18121 cactgaatac ggcaattgat aaggagttgt cccgtacaac cagcgatgcg ctgtttctgg
    18181 cgctgcgccg ggtgaaagca gatctgaatg cggatatcaa cacgcgcctt gaacagtctg
    18241 cacggatcat tcagcgcaca ccggatgagg ttttacccgc gctggtgctg gcggagacct
    18301 ggtttgataa cgcggcgcgt gacgcggaca ttatccggcg taatgccatt acgcatcccg
    18361 gctttgtgcc ggtgatccct ctgaaggtgc cagtgcaatg aacgacaatg tcacgctacg
    18421 ggtaaatggc cgggagtgga atggctggac attggtgcgc atcggtgccg gtattgaacg
    18481 gctggcgcgg gatttcagtg tggagatcac ccgccagtgg ccgggagatg agggtattac
    18541 cacgcttcag ccgcgcatta aaaacggttc aaaagtggag gtgctgattg gtgatgagct
    18601 ggtgatcacc ggctgggtgg aggcgacgac cgttcgttac gatgcccgtt cggtcatcac
    18661 cggtattgcc ggacgcagtc tgaccgctga cctgattgac tgtgcagccg aaccgacaca
    18721 gtttaacgga cgatcgctgg tacagattgc gcaggcgctt gctgcgcctt tcggcattga
    18781 ggtggtgaac agcggtgcgc cgtcgggtgt tattcctgat gttcagcctg atcacggtga
    18841 aacggtgatt gaggtaatca acaaaatact cggtcagcag caggcactgg cttacgacga
    18901 cccgcacggc aggctggtga ttggcggtat tggctcaacg cgggcacata ctgcgctggt
    18961 actcggggaa aacatccttt cctgcgatac ggagaagagt atccgggagc gattttctgt
    19021 ttaccaggtg gcggggcagc gtgccggaaa tgacgatgat ttcggtgagg ccaccaccac
    19081 cgcgctgcgg gcccgtacag aggacgcatt tattgcccgt taccgtccga tgtatatcag
    19141 gcagacaggg caggctacgg gggcaggctg tattgcgcgt gctgactttg aagcccggca
    19201 acgggcggcg cggacggatg aaaccaccta tgtggtgcag ggctggcgac agggtaacgg
    19261 tacgctgtgg cagcccaacc agcgggtgat tgtctttgat ccggtctgtg gtttcgacaa
    19321 taccgaactg cttgtttcgg aagtcacgtt tactcaggac cagaacggca ccctgacgga
    19381 aatccgtgtc ggcccgcctg atgcttatct gcctgaaccc gaagcccccg gcgcgcggaa
    19441 aaagaaaaaa gccagagtac aggaggaccc gttctgatga ggacgattga agccatgcag
    19501 cgacaactcc tcggcctgat tgggcgggca gtggtgaaaa gcatcagcgc cgccacgaaa
    19561 tgtcagaccg tggatgtgtc cctgattgcc ggtgaaccca aagccgggat tgaacatctt
    19621 gaaccctacg gttttaccgc aagggcaaac agcggtgcgg aagcggtggt gttgtttccg
    19681 gatggcgacc gttctcatgc ggtggttgtt acggtgtcgg accggcgcta ccgcctgaaa
    19741 gggctgcaga cgggtgaggt ggctgtctat gacgatcagg ggcagtctgt gacgctgacc
    19801 cgggagggga tcgtggtgga cggtgcaggt aaaacgatca cgtttcgcaa tgcgcctaag
    19861 gcacgttttg aaatggacct ggaagtgaca ggacaggtga aagacctgtg cgactccggc
    19921 ggcaccacca tgtcagcgat gcggcttgcc tataacgggc atcgtcacag agagaacggt
    19981 cagggcagta acaccgacaa acctgataaa gcgatggggg catgatggaa ctgtggctga
    20041 cggtgaacgg taaacgcacc tgcgccagcg caccgctgga tccgctgacc cgcgccgtgg
    20101 tgatttccct gtttacctgg cggcgggcgg agcctgatga caacgccgac gtcccgatgg
    20161 gatggtgggg ggatacctgg cctgcggtac agaatgaccg ttacggctcc cgactgtggc
    20221 tgcttcagcg cagcaaactg accaatcagc tggtgcagac ggtaaggggg tatatccgcg
    20281 aatgcctgca atggatgatt gatgacggcg tggtgtcccg tattgatctg gatatccgcc
    20341 gcaccgggat taatgaactg ggtaacagta tcactctctg gcgtcgtgac ggaccggtaa
    20401 tgatttcttt tgatgatctg tggagtgcga taacgcatgg cggacagtga atttcagcgc
    20461 ccgacgctgg cagaaaatat cagtatgctc cgtaacgatt tattcgtcag gctggacgtc
    20521 agcgacacgc tccggcgcat ggatgaagac gtgcgggcaa aggtgtatgc ggcggcgctg
    20581 catacggtct acgggtacat cgattatctg gcaatgaata tgctgcctga cctgtgcgat
    20641 gagtcctggc tggcgcgaca tgctgcgatg aaacggtgtc cgcgcaaggg ggccacggct
    20701 gccagcgggt atatgcgctt ggaaggtgtc agcgatggcc tgaaggtgac tgccgggagc
    20761 gtgattcagc gcgatgacct ggttcagtac acggcaactg ccgatgcaac cagctccggt
    20821 ggtgtcctgc gtgtgccgat cacttgctca actacaggcg cggtcggtaa cgctgacgac
    20881 ggtacggcat taatcctggt cacgccggtg aatggtctgc cgtcttccgg tgttgcagat
    20941 accctgactg gcggattcga tactgaagat ctggaaacgt ggcgcgcccg ggtcattgag
    21001 cggtattact ggacgccgca gggcggggct gacggggact atgtcgtctg ggctaaagaa
    21061 gtgcccggca ttacccgtgc atggacatac cgtcactgga tgggaacggg aactgttggt
    21121 gtgatgattg ccagcagtga cctgattaac cccattccgg aagaatcaac ggaaacggcg
    21181 gcaagacaac atatcgggcc actggccccg gtggcaggct ctgatttgta tgtgttcagg
    21241 ccggtggcac atacggtgga ttttcatatt cgcgtgacgc cggacacacc ggaaatacgg
    21301 gctgccatca ccgcggagtt gcgttcgttc ctgctgcgtg atggttatct gcagggagaa
    21361 ctgaaggtat cgcgtatcag tgaggcgatt tccggtgcga acggggaata cagccatcag
    21421 ttgcttgcac cggcagacaa tatctccatt gcaaaaaatg aactggcggt tctggggacg
    21481 atttcatgga cgtgacaaac gatgattaca tccgtctgtt gtcggcactg ctgccgcccg
    21541 gtccggcgtg gtcagccagc gatccggcga ttgccggtgc ggcacagtca ttaacccgcg
    21601 ttcatcagcg tgcggatgcc ctgatgcggg agctggatcc gcgcaccacc accgaactga
    21661 taaatcgctg ggagcgtctg tgcggcctgc cggatgaatg tattcccgca ggaacacaga
    21721 cccttcgcca gcgtcagcaa cgactggatg cgaaggttaa cttggcgggc ggcatcaatg
    21781 aggattttta ccttgcacag cttgctgccc tgggcagaca agacgccacc atcacgcgat
    21841 acgacaaaag cacgttcacc tgctcatcgg cctgtactga cgcggtgaat gcgccggaat
    21901 ggcggtatta ctggcaggtc aacatgccag ccgccaccaa cagcacctgg atgacatgtg
    21961 gcgatccctg tgattccgcg ctgcgtatct ggggcgacac cgtcgtcgaa tgtgtgctta
    22021 acaaactctg cccttcgcat acctacgtaa tttttaaata tccggagtaa tccatgcatc
    22081 gtatagacac gaaaaccgcg cagaaggata agttcggcgc gggtaagaac ggttttaccc
    22141 gtggtaaccc ccagactggc acgcttgcca ccgatctgga tgatgactac tttgacatgt
    22201 tgcaggagga gctttgcagc gtggtggagg catccggtgc cagcctggag aaggggcggc
    22261 acgatcagtt gcttaccgca cttcgcgcgc tgctgttaag ccgcaagaat ccgtttggcg
    22321 atatcaaatc ggatggcacg gtgaaaacgg ctctcgaaaa ccttggtttg ggagaagcag
    22381 ctaaaaggaa tgtaggtaca ggggccgaa
//
LOCUS       DADLSE010000053        22345 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLSE010000053 DADLSE010000000
VERSION     DADLSE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.053"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(2..94)
                     /locus_tag="JN692_002084"
     tRNA            complement(2..94)
                     /locus_tag="JN692_002084"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN692_002085"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="JN692_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7264399.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN692_002086"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="JN692_002086"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7264400.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKQEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLGRTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVTEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN692_002087"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="JN692_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7264401.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN692_002088"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="JN692_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7264402.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN692_002089"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="JN692_002089"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7264403.1"
                     /translation="MTDSELMQLSEQVGQTLKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN692_002090"
     CDS             complement(6113..7198)
                     /gene="mltB"
                     /locus_tag="JN692_002090"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7264404.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7454..8017
                     /gene="srlA"
                     /locus_tag="JN692_002091"
                     /gene_synonym="gutA"
     CDS             7454..8017
                     /gene="srlA"
                     /locus_tag="JN692_002091"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7264405.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="JN692_002092"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="JN692_002092"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7264406.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGIKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="JN692_002093"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="JN692_002093"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7264407.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="JN692_002094"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="JN692_002094"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7264408.1"
                     /translation="MNQVAVAIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="JN692_002095"
                     /pseudo
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="JN692_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="JN692_002096"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="JN692_002096"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7264409.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="JN692_002097"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="JN692_002097"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7264410.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JN692_002098"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="JN692_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7264411.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEMVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="JN692_002099"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="JN692_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7264412.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLNLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELTSEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="JN692_002100"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="JN692_002100"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7264413.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JN692_002101"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="JN692_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7264414.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWLAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JN692_002102"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="JN692_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7264415.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20889)
                     /locus_tag="JN692_002103"
     CDS             complement(19876..20889)
                     /locus_tag="JN692_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7264416.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            21146..>22345
                     /gene="ascF"
                     /locus_tag="JN692_002104"
     CDS             21146..>22345
                     /gene="ascF"
                     /locus_tag="JN692_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7264417.1"
                     /translation="MAKNYAALARSVIAALGGVNNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISCFICG"
BASE COUNT         5242 a         6200 c         5687 g         5216 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggtt accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt cccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tcctgtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttaccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtata
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcactgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgtctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc tcttgtaaat agctactgta
     6121 ctcgcgccag cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt
     6181 ggttgtaacg ggtgatggtg tagaagttcg gcagaccgta ccagtactgg tagccggtgc
     6241 caacatccag acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta
     6301 aacctgcggc ggcaagctgc gaaatgctgt acttagtttt gaaaccattt ggcaagcctg
     6361 gagcctgacc gtttgccatt atcgcgacct gatcgccttt cacccagccg tgcgctttga
     6421 aatagttcgc cacgctaccg atcgcatcaa ccggatccca caggttgata tgcccgtcgc
     6481 cgctgaaatc taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg
     6541 ccccggcaaa ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca
     6601 ggaaggtttc cagctcgcca gagaaatact ccgcgcggcg tgggtagtta aatgacagcg
     6661 ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc
     6721 cgataatccc gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg
     6781 catcttcata ctgattccag aaaaccacac cgttctgcac gttgtccggc gtaataaatt
     6841 ttttgcgata acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg
     6901 gtgcctggtt atccatcagc cgcagtaccg aatccagacg cttcgcctgg gagagaattt
     6961 cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg
     7021 cattcgggtt attagcgaaa tcgccgccca tctgcatcac attgtgctgc ggctcaagca
     7081 ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc
     7141 tacaggcggc aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga
     7201 ggggtccatt taacagattc aaccaggggc aagtatggta aagcatcacg ccccgcacaa
     7261 ggaagcggta gtcactgccc gatacggact ttacataact caactcattc ccctcgctat
     7321 acttttattc aaactttcaa attaaaatat ttatctttca ttttgcgatc aaaataacac
     7381 ttttaaacct ttcaatctga ttagattagg ttgccgtttg gtaataaaac aataaatcct
     7441 gaaggagaga acaatgatag aaaccattac tcatggtgca gagtggttta tcgggctgtt
     7501 ccaaaagggc ggagaggtgt ttaccgggat ggtgaccggc attcttccgc tgttgattag
     7561 cctgctggtt atcatgaacg cactgattaa ttttatcggt cagcatcgta ttgaacgttt
     7621 tgctcaacgt tgcgccggta accctgtttc ccgttaccta ctgttaccgt gcattggcac
     7681 gtttgtcttt tgcaatccga tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa
     7741 acccagctac tacgcggcgg cctcttatag ctgccactca atgaatggcc tcttccccca
     7801 tatcaaccct ggcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcataa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctggcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcctgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgcca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcc gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgccta gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtggg ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagatggttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacgcggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc accatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcggtggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcaacctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactga catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccgg tgccagccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacat gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg
    21001 aacaattaac tctcttgctt atttagtgac aactattcat gattttgtga aaccggtttc
    21061 ttaattccgt ttcagcatag gcatttttcc gtcacgtcga ctgataacaa ctacatctac
    21121 cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt
    21181 gatagcggca ctgggcggcg ttaataacat ctcggcagtc acgcactgta tgacgcgttt
    21241 gcgctttgtt atcaaagatg accagcttat cgacagcccg acgttaaaaa ccatccccgg
    21301 cgtgctcggc gtggtacgta gtgacaacca gtgtcaggta attatcggca atacggtttc
    21361 gcaggccttt caggaagtcg tcagcctgct gccgggagat atgcagcccg cacagcccgt
    21421 gggtaaaccc aaactcacgc tgcgtcgcat tggtgcgggg atcctcgatg cgctgatcgg
    21481 caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat
    21541 gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca ttctgaatgt
    21601 gattggtgac ggtgctttct tcttcctgcc gctaatggtc gcggcatctg ccgccatcaa
    21661 atttaaaacc aatatgtcgc tggcgattgc catcgcgggt gtgctggtac atccgagttt
    21721 tattgaatta atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgatcccggt
    21781 caccgcggtg aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc
    21841 atatatcgaa cgctgggtgg atcgcattac accggcggtg accaaaaact tcctcaagcc
    21901 aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat
    21961 ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct
    22021 ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgcgt
    22081 ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggaa tggtcatgcc
    22141 atcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgc cgtggaaaac
    22201 gaaaaacccg gaactgcgcc agacggcgct ggctgcggcg gcatcagcca ttatggcggg
    22261 gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc ttatcgccag
    22321 tcttatcagc tgttttattt gcggc
//
LOCUS       DADLSE010000054        22176 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLSE010000054 DADLSE010000000
VERSION     DADLSE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.054"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            139..1320
                     /gene="nimT"
                     /locus_tag="JN692_002105"
     CDS             139..1320
                     /gene="nimT"
                     /locus_tag="JN692_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HAY7264418.1"
                     /translation="MTCSTSLSGKNRIVLIAGILIIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            1375..1722
                     /locus_tag="JN692_002106"
     CDS             1375..1722
                     /locus_tag="JN692_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7264419.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKNQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN692_002107"
     CDS             complement(1744..1998)
                     /gene="yoaF"
                     /locus_tag="JN692_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7264420.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            2181..2738
                     /gene="dgcP"
                     /locus_tag="JN692_002108"
                     /pseudo
     CDS             2181..2738
                     /gene="dgcP"
                     /locus_tag="JN692_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
     gene            2782..3747
                     /gene="yeaX"
                     /locus_tag="JN692_002109"
     CDS             2782..3747
                     /gene="yeaX"
                     /locus_tag="JN692_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7264421.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN692_002110"
     CDS             complement(3801..4916)
                     /gene="rnd"
                     /locus_tag="JN692_002110"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7264422.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN692_002111"
     CDS             complement(4998..6683)
                     /gene="fadD"
                     /locus_tag="JN692_002111"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7264423.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEVKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN692_002112"
     CDS             complement(6888..7469)
                     /gene="yeaY"
                     /locus_tag="JN692_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7264424.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTLQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN692_002113"
     CDS             complement(7509..8204)
                     /gene="tsaB"
                     /locus_tag="JN692_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7264425.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8262..10172)
                     /locus_tag="JN692_002114"
     CDS             complement(8262..10172)
                     /locus_tag="JN692_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7264426.1"
                     /translation="MTDDFAPDGQLAKAIPGFKSREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQRALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10304..10648
                     /locus_tag="JN692_002115"
     CDS             10304..10648
                     /locus_tag="JN692_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7264427.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11010..11369
                     /locus_tag="JN692_002116"
     CDS             11010..11369
                     /locus_tag="JN692_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7264428.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11489..11668)
                     /locus_tag="JN692_002117"
     CDS             complement(11489..11668)
                     /locus_tag="JN692_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7264429.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            11742..13103
                     /gene="pabB"
                     /locus_tag="JN692_002118"
     CDS             11742..13103
                     /gene="pabB"
                     /locus_tag="JN692_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7264430.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            13107..13685
                     /locus_tag="JN692_002119"
     CDS             13107..13685
                     /locus_tag="JN692_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7264431.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            13869..15233
                     /gene="sdaA"
                     /locus_tag="JN692_002120"
     CDS             13869..15233
                     /gene="sdaA"
                     /locus_tag="JN692_002120"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7264432.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            15364..16962
                     /locus_tag="JN692_002121"
     CDS             15364..16962
                     /locus_tag="JN692_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7264433.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN692_002122"
     CDS             complement(16966..18516)
                     /gene="yoaE"
                     /locus_tag="JN692_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7264434.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            18979..19950
                     /gene="manX"
                     /locus_tag="JN692_002123"
     CDS             18979..19950
                     /gene="manX"
                     /locus_tag="JN692_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7264435.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20013..20813
                     /locus_tag="JN692_002124"
     CDS             20013..20813
                     /locus_tag="JN692_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7264436.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            20826..21677
                     /locus_tag="JN692_002125"
     CDS             20826..21677
                     /locus_tag="JN692_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7264437.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            21732..>22176
                     /locus_tag="JN692_002126"
     CDS             21732..>22176
                     /locus_tag="JN692_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7264438.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         5352 a         5757 c         5572 g         5495 t
ORIGIN      
        1 tcggtcgggt tcatagccat tgagattcaa cctgtgcatc attttgtccg aacttagcga
       61 taatttgtca ttttagcttg attcaacata acaataaaaa cggtaaggta cagcctcgtt
      121 tgtaacaatg agaagcatat gacctgttca acttcattaa gcggcaaaaa caggattgtc
      181 cttatcgctg gcattctgat aattgccaca acattacgcg tcacctttac cggcgcagcg
      241 ccgttactgg atacgattcg ttccgcttac tcgctgacga cagcgcaaac cggcttatta
      301 accaccctgc cattattggc ctttgcgcta atctcacctc tggctgcccc ggtggcgcga
      361 cgttttggta tggaacgtag cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca
      421 attcgttctc tcccttcgcc ttacttatta tttggcggta cagcggtcat tggcggtggg
      481 attgcattag gcaatgtctt actgccagga ttaattaaac gcgatttccc tcattccgtc
      541 gccagactta ccggcgcata ttccctgaca atgggagctg cagcggcact gggatcggct
      601 atggtcgtgc cgctggcttt gaacggtttt ggctggcaag gcgcgttgtt catgctgatg
      661 tgttttcctc tgctggctct ttttttatgg ctgccacagt ggcgaagtca gcaacatgca
      721 aatttgagta cctcacgcgc cttacatact cggggtatct ggcgttcgcc acttgcctgg
      781 caggtaacat tgtttcttgg gatcaactca ctggtctatt acgtgattat tggctggctt
      841 ccggcgatcc tcatcagtca cggctatagc gaagcacagg cgggttcact gcatggtttg
      901 ctgcaactag ccacagcagc tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa
      961 gaccagcgtg gtattgcagc gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc
     1021 tgctttatgc cagcgcacgc gatcacctgg actctgcttt tcggttttgg ttccggcgca
     1081 acaatgatac tggggttgac gttcattggt cttcgggcaa gttctgcgca tcaggcggcg
     1141 gcactctcgg ggatggcaca atccgtcggg tatttgctgg cagcctgtgg gccgccgcta
     1201 atgggtaaaa tacacgatgc taacggtaac tggtccgtac cactgatggg tgttgcaata
     1261 ctttcactac tgatggcgat tttcggactt tgcgccggga gagacaaaga aattcgctaa
     1321 tatccggtgc tatagtgacg tgacaaatct tgcgtgaaag ggagaacaaa cacgatgaat
     1381 attcagtgca aacgcgttta tgatccggct gaacagagcg atggttatcg cgtactggtc
     1441 gaccgcctct ggccgcgcgg tatcaaaaaa accgatttag cccttgatga gtgggataaa
     1501 gaaatcacgc cgtcaacgga actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca
     1561 acctttcgcg agcaatatcg cgcagaactg gcgcaacacg agcaagaagg aaagcggctg
     1621 gcggacatcg ccaaaaacca gccgctgacc ctgctctacg cagcaaaaaa caccacgcag
     1681 aaccatgcgc tggtgctggc cgactggcta cgtagcttgt gattttagta cggcatccgg
     1741 cggttatttt tcaccagccg gatggtcccg tcgccacaat gcccattcat caatcgtttc
     1801 accgcccggt aatttgcaat tgttgctgac cccttgcgct gtctgcactg gaatgagcgt
     1861 cccgcccttc tgctggcaat agaccgacgc cggatttgcc ataccaatct gcggcggttt
     1921 aggtgcttct ggctgagaag gggttgaaca accagccagg accagcaagc aaggcagaac
     1981 aaaactgata attttcattt attgatctca catatttatc caagcttaga gtatcgcggt
     2041 atcgttttgt tttgcagcac tatttttatt acattcactc aaaacatatt acgtcttgtt
     2101 tcatctttgt tgatgatgtt ttatcatgcc tgcaaagatt aaataatcag cacttacccg
     2161 ccgtatcctg gagttgttcc gtgtcagatc agattatcgc ccgcgtctcg caatcccttg
     2221 ccaaagaaca gtcactggaa agtctggtcc gacagcttct ggagatgctg gaaatggtca
     2281 ctgatatgga atcaacctac ctgaccaaag tggatgtcga agcgcgcctg cagcatatta
     2341 tgtttgcccg taacagccag aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt
     2401 actcgttatg caaacgcgcc attgatgaaa actgcttttt cagcgatgaa gtccctgacc
     2461 gttggggcga ctgtattgcg gcacgcaatc ttggcatcac cacatttctg agcacgccaa
     2521 ttcacttacc ggatggatca ttctatggca cgctttgcgc cgccagcagt gagaagcgcc
     2581 agtggagtga acgcgcggaa caggttttac agttattcgc cggactgatt gcacaatata
     2641 ttcaaaaaga ggcactggtt gaacagctgc acgaagccaa tgctgcgctg attgcgcaat
     2701 cgtataccga ctcgttaacc gggctaccga atcggcggcg gtattttccg ccgctgggct
     2761 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagccag
     2821 gttgaacccc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
     2881 ctgcctgcgt tcaccggagg aagtcacatc ctagtgcaga tgagcgatgg tgataaccag
     2941 tacagcaatg cgtattcact actgagttca ccgcataaca cctctagtta tcagattgcc
     3001 gttcgactgg aagaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
     3061 ggcgatcggt taacgatctc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
     3121 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
     3181 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
     3241 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
     3301 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
     3361 gtttatacct gtggccccga ggcgctaatt gaagcggtaa gaagtgaagc tgcgcgtctg
     3421 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
     3481 gcatttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
     3541 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
     3601 gtatgcggaa cctgcgaaac ggcaatactg gaaggtgaag ctgaccatcg ggatcagtat
     3661 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
     3721 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
     3781 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
     3841 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
     3901 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
     3961 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
     4021 taaccaggca tgtccatcag gttaagcatc ggctgcggta aggcatcttc cggcaatgtc
     4081 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
     4141 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
     4201 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc
     4261 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaactgcca ggcattggtg
     4321 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
     4381 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
     4441 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
     4501 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
     4561 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
     4621 gtgtcaatca agggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
     4681 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
     4741 cagtcggtga tcccgagagg atcaattagc gccagatgct cgccatcgaa aagttgaatc
     4801 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc
     4861 ggaaaggcac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
     4921 taattcaaat tgttttctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
     4981 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
     5041 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
     5101 ttcggtactt tgtatcccgt gagctgacgg cggcaaaaag tcaccagtga ctcttcggta
     5161 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
     5221 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
     5281 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
     5341 aggaatcctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
     5401 atctcatctg tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca
     5461 caaagctcac ccggttgacc cggtggtact tcgttgtcgt cgtcatccac cagtttgact
     5521 tccgtcgatg gcaccggcaa accgatgcta ccgctatgat aatcaatatc atacgggtta
     5581 acgctcacca gcggcgcgca ctcggtcagg ccataacctt ccagcagata ctgtccggtc
     5641 agtttcaccc agcgttctgc caccacttgc tgaacaggca tcccaccgcc tgcggaaaga
     5701 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
     5761 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
     5821 atatcacgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
     5881 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
     5941 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
     6001 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
     6061 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
     6121 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
     6181 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
     6241 attacgtgct gaacggcggt tttatcaacc actttttcca gagtgtgggc aaagttagac
     6301 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
     6361 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa tagcgccacc
     6421 ggatattgca ataagttagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
     6481 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatc
     6541 acctccccca tattcacaaa cgcaggctga tcggcgtagc gcgcgaccga ctgctcaaac
     6601 atatctacca gagattgata acggtcaggg ttgatctccg tcggaacgtc cgcgggataa
     6661 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
     6721 accccaaatg atatacatgc tgttaacata atattaactc atcataccag cttgataatt
     6781 acccaacgaa aaggttgcga agcgcgtcac tatttatttt tatctttacc gtaagaatgc
     6841 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caagcaatta ttcggttaca
     6901 actgtttgta ctctcgcggg gccgggatta taccagcccc atccgccgta gccatagggc
     6961 cagccacgac cgccataaaa ccatggatca atcggctgag gcggcataat cacctgcagg
     7021 gttaaatgcc aacgtttgta acccgttact tgcatcacca taaatttata gggcgtattg
     7081 ccgattttgc cgtcaaccgc accggtgatt ggcccgacta ccgtaaccag ttgtccacgg
     7141 aaatccaccg ggtccagaaa accgttcaca tcggcataaa tgcgaccgcg agaaggttct
     7201 cccagcgtcg gcctggctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
     7261 ccttgctggt tttgtaccgc aaccactttg ccaccaaagc gtgcctcctg accaacgtac
     7321 agctgcggcg cactcatcac ccgaactaaa tcttgttgcg gcgtgggact gctgccttta
     7381 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
     7441 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
     7501 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg caaataaacc
     7561 ggctcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca agcaatcggc
     7621 agcatatctt ctgcagcggg gagtaacact tcgccatcgc gcaaaaccag cccgctctct
     7681 ttaccgagat ccggccaggc ttgccagccc gtccctaccg tcacccattc gccggaaagc
     7741 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgtgc
     7801 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
     7861 atggctgaca gcacgcgtgt tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt
     7921 agcgtggaga cgccaatcat cggtaattcc gcgccaagcg ccagcccttg cgcgatgcca
     7981 ataccaatgc gcacgccggt aaagctaccg gggccgcgcc cgtaagccag agcgttaata
     8041 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
     8101 gtatgttcac gagggcaaag ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg
     8161 acagagcagg cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
     8221 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
     8281 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
     8341 agaaacgtcg cgccgtaagg acgcatcacc agccgatagt cacaaatcac cagcacgcca
     8401 cgatcgtcgg cgtcgcgaat cagtcgacct accccctgtt tgagagtaat gacggcatct
     8461 ggtagttgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
     8521 tttaacagtg gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
     8581 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcatta
     8641 ccggcactga caaactgctg caacagttgg cctttactgg tttccccctg caacaaaacg
     8701 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc gtgcgaggta
     8761 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgctaac
     8821 tggcgagcag aacctggctg gttggtttgc ggcagattgc gcggcacaca gagtaacgcc
     8881 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
     8941 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
     9001 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
     9061 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
     9121 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
     9181 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccaa ggtgtcatcg
     9241 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
     9301 taacctggct caccgagttg cagacgaaaa tcctgcgcac tctgggcaag acgatcagcg
     9361 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
     9421 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
     9481 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
     9541 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
     9601 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
     9661 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
     9721 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
     9781 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcccg ctgttcgaga
     9841 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
     9901 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
     9961 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    10021 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    10081 acggctaccg ccatctgtcg ctgtggttct cgcgacttaa agcctggtat cgctttcgcc
    10141 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    10201 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    10261 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    10321 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    10381 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    10441 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    10501 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    10561 gggtcatgcg ccggtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    10621 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    10681 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    10741 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    10801 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    10861 tcttagctga acaatctggt tatagcgtgc gggctgaccg ttaattttct ccatcttact
    10921 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    10981 ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat agccttatcg
    11041 ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa gccctggatt
    11101 tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa agcacggcga
    11161 agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct gatattacgg
    11221 cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt ggatgggcaa
    11281 aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt tcaacatata
    11341 tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa atgggtcagg
    11401 acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat catcggcttc
    11461 tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa cgcgccacga
    11521 ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt gcctggccgc
    11581 tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc tgctgttcat
    11641 gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt ggcgctaatt
    11701 atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg ttatctcccg
    11761 ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc cgcttaagcc
    11821 acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat agccgctttg
    11881 atattgtggt cgccgatccg atttgcactt taaccacttt aggtaaagaa accgttgtta
    11941 gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc cagcaggtgc
    12001 tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag ggcggcgcac
    12061 tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa attgcggaac
    12121 aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc attgtcgacc
    12181 accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt cgggcctggc
    12241 tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac tggcaatcca
    12301 atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat ctgcacagcg
    12361 gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct ggcgatgaat
    12421 ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct tttttacgtc
    12481 ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt gataatagtg
    12541 aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct caggaagata
    12601 gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat ctgatgattg
    12661 tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta aaagtaccag
    12721 agctcttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact ataacggcgc
    12781 gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct ggtggctcaa
    12841 taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa ccgcagcgac
    12901 gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg gataccagca
    12961 ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg ggcggtggaa
    13021 ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa gttaataaga
    13081 tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt gatgattttt
    13141 tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat catcgtcagg
    13201 ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg actcagcgtt
    13261 caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca gtcgatgaca
    13321 cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc gctataccgc
    13381 cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact ggctatcagg
    13441 taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt gaagatgaag
    13501 tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt tatcaccctt
    13561 tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac gaacagtatt
    13621 ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt ggtgtgaacc
    13681 cctgactata cttatcttta catctacaaa acactacttg agacaatcat cgcaatatta
    13741 gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag tcgccgcgtt
    13801 ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac cttgtcagga
    13861 gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc tcatcttccc
    13921 ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc gaaaaaggct
    13981 tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg ctgacgggta
    14041 aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa cctgccaccg
    14101 tggatatcga cagtattccc ggttttattc gcgacgtaaa agagcgcgaa cgtctgctgc
    14161 tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt tttcataacg
    14221 gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc gatgaagtcg
    14281 tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa gaacactttg
    14341 gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc accgaactgc
    14401 tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag aacgaactgg
    14461 cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa accatgcagg
    14521 catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg cgcgtgccac
    14581 gtcgtgcgtc tgccctgcgc cggatgttag tttccagcga taaactgtct aacgatccga
    14641 tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa aacgccgccg
    14701 gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg gcagtgctgg
    14761 cttactatga ccactttatt gaatcggtca gcccagacat ctatacccgt tactttatgg
    14821 cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt gcggaagttg
    14881 gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt gcagaactgc
    14941 tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa cacaaccttg
    15001 gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag cgtaatgcca
    15061 ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc agtgcaccgc
    15121 gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac atgaacgcca
    15181 aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac taatacttct
    15241 tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct gctgtaatat
    15301 tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta cagggtggcc
    15361 cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat gattgtttgt
    15421 acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat ttcacagcgt
    15481 aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga attagataaa
    15541 gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg tctgccctgc
    15601 tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt gcgatccatt
    15661 ggcctggtgc aagacggcac actttattgc tccagcattt ttggttatcg caatgtgccc
    15721 gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct gacgatcgac
    15781 cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc gggcagtagc
    15841 aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct ccttgagcca
    15901 caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt gctctatggt
    15961 aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca ggtttcttcg
    16021 caaagctttc cttttaccat taacgtaaat ggtccggggg ctacggcgct ggcatggcac
    16081 tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg ctatatcgcc
    16141 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa ttaatctggg cctggcgcaa
    16201 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
    16261 gtagagattt tgctgcgctg gaataatccg cgtcagggct ggatttcacc ggatgtgttt
    16321 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
    16381 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
    16441 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
    16501 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
    16561 gattatcgga ttgcccgcga gctgcatcgt aaaaacgtca aactggcgat tgatgacttc
    16621 ggcaccggca acagctcgtt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
    16681 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
    16741 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
    16801 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
    16861 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
    16921 cggcataatg gacatatcac gcccattatg tcgttacgtt aacgcttact catcttcatc
    16981 gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat tggcttcaag
    17041 gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa tgtgaccatt
    17101 tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac
    17161 atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc agccgttacc
    17221 atcggtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa tggcttccag
    17281 cacatccagc ggcgtgacca agccttgtac cacaccaaac tcgttggtca cgataacaaa
    17341 gctcccgcga gcgcgacgca gcacgcccag caggttgatc ggatcgaggg tttccgggac
    17401 gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt ccagcgccac
    17461 cagcagttct ttagcacgta cgataccgat gatttcatcc agttcaccgc gacataccgg
    17521 gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc cgagattagc
    17581 gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac gcgacgccag
    17641 cgtcagtacg ccgttaatca tgtaccgttc ttcttcggca aatgcacctt ccgggatcgg
    17701 catcgggtta tcggcatcgt gctgaacatt ggcctgacgt ttcccgccca tcaaacgcag
    17761 gatggcatcg gcagtacgcg ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg
    17821 acgcgcaatc tggttaaaca cttcgataat gatcgagaag ccaatcgcgg catacaggta
    17881 acctttcgga atgtggaaac cgaaaccttc tgccaccaga ctcagaccaa tcattaacag
    17941 gaagctcaga cagagcacca ccaccgtggg gtgctggtta acgaatcgcg tcagcggttt
    18001 cgatgccagc aacataaccg ccatcgcaat cactaccgcc gccatcatca ccggcagatg
    18061 gttaaccatc cctactgcag taattaccgc atccaacgag aagacggcgt caaggatgac
    18121 aatctgtgtg acgaccaccc agaaactggc gtagccttta ccgtggccgg aatcatgatc
    18181 gcggttttcc agccgttcat gcagttcggt tgttgctttg aacagcaaga atatcccccc
    18241 gaacaacata atcagatcgc gtccggagaa ggagaaatcc atgacggtaa atagcggttt
    18301 ggtcagcgtg accatccatg aaatcagcga cagcagcccc agacgcataa tcagcgccag
    18361 tgataacccc agcaaacgcg ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat
    18421 ggcgatgaag accaggttat cgatacccag cacaatttcg agaacaacaa gcgtgagtag
    18481 ccccgcccaa attgaggggt ccattaagaa ttccatgaca agctcctgct taaggaatag
    18541 ctattcgacg ccagaaataa tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat
    18601 agagtgccag aaaggcaggc gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc
    18661 atactgcggg ctactgccct atactccatg gttgttaaac gggagttaaa catatcagag
    18721 acgcctctga tttggcaaag atttaccttc ctttgcaaac gaatgtgaca aggatatttt
    18781 acctttcgaa atttctgata atcgaaagtt aaattacgga tcttcatcac ataaaataat
    18841 tttttcgata tctaaaataa atcgcgaaac gcaggggttt ttggttgtag cccttatctg
    18901 aatcgattcg attgtggacg acgattcaaa aatacatctg gcacgttgag gtgttaacga
    18961 taataaagga ggtagcaagt gaccattgct attgttatag gcacacatgg ttgggctgca
    19021 gagcagttgc ttaaaacggc agaaatgctg ttaggcgagc aggaaaacgt cggctggatc
    19081 gatttcgttc caggtgaaaa tgccgaaacg ctgattgaaa agtacaacgc tcagttggca
    19141 aaactcgaca ccactaaagg cgtgctgttt ctcgttgata catggggagg cagcccgttc
    19201 aatgctgcca gccgcattgt cgtcgacaaa gagcattatg aagtcattgc aggcgttaac
    19261 attccaatgc tcgtggaaac gttaatggcc cgtgatgatg acccgagctt tgatgaactg
    19321 gttgcgctgg cagtagaaac aggccgtgaa ggcgtgaaag cactgaaagc caaaccggtt
    19381 gaaaaagccg cgccagcacc cgctgccgca gcaccaaaag cggctccaac tccggcaaaa
    19441 ccaatggggc caaacgacta catggttatt ggccttgcgc gtatcgacga ccgtctgatt
    19501 cacggtcagg tcgccacccg ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt
    19561 agtgatgaag tggctgcgga taccgttcgt aagacactgc tcacccaggt tgcacctccg
    19621 ggcgtaacag cacacgtagt tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa
    19681 tatgctggcg aacgcgtaat gctgttattt accaacccaa cagatgtaga gcgtctcgtt
    19741 gaaggcggcg tgaaaatcac ctctgttaac gtcggtggta tggcattccg tcagggtaaa
    19801 acccaggtga ataacgcggt ttcggttgat gaaaaagata tcgaggcgtt caaaaaactg
    19861 aatgcacgcg gtattgagct ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg
    19921 atggatctga tcagcaaaat cgataagtaa cgtattgtgt tgattatcat tcagttttca
    19981 cacttaagtc ttacgtaaac aggagaagta caatggagat taccactctt caaattgtgc
    20041 tggtatttat cgtagcctgt atcgcaggta tgggatcaat cctcgatgaa tttcagtttc
    20101 accgtccgct aatcgcgtgt accctggtgg gtatcgttct tggggatatg aaaaccggta
    20161 ttattatcgg tggtacgctg gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag
    20221 ttgcgcctga cgccgctctg gcttctatca tttctaccat tctggttatc gcaggtcatc
    20281 agagcattgg tgcaggtatc gcactggcaa tccctctggc cgctgcgggc caggtactga
    20341 ccatcatcgt tcgtactatt accgttgctt tccagcacgc tgcggataag gctgctgata
    20401 acggcaacct gacagcaatt tcctggatcc acgtttcttc tctgttcctg caagcaatgc
    20461 gtgtagctat tccggccgtc atcgttgcgc tgtctgttgg taccagcgaa gtacagaaca
    20521 tgctgaatgc gattccggaa gtggtgacca atggtctgaa tatcgccggg ggcatgatcg
    20581 tggtggttgg ttatgcgatg gttatcaaca tgatgcgtgc tggctacctg atgccgttct
    20641 tctacctcgg cttcgtaacc gcagcattca ccaactttaa cctggttgct ctgggtgtga
    20701 ttggtactgt tatggcagtg ctctacatcc aacttagccc gaaatacaac cgcgtagccg
    20761 gtgcgcctgc tcaggcagct ggtaacaacg atctcgataa cgaactggac taacaggtga
    20821 gcgaaatggt tgatacaact caaactacca ccgagaaaaa actcactcaa agtgatattc
    20881 gtggcgtatt cctgcgttct aacctcttcc agggttcatg gaacttcgaa cgtatgcagg
    20941 cactgggttt ctgcttctct atggtaccgg caattcgtcg cctctaccct gagaacaacg
    21001 aagctcgtaa acaagctatt cgccgtcacc tggagttctt taacacccag ccgttcgtgg
    21061 ctgcaccgat tctcggcgta accctggcgc tggaagaaca gcgtgctaat ggcgcagaga
    21121 tcgatgacgg tgctatcaac ggtatcaaag tcggtttgat ggggccgctg gctggtgtag
    21181 gcgacccgat cttctgggga accgtacgtc cggtatttgc agcactgggt gccggtatcg
    21241 cgatgagcgg cagcctgtta ggtccgctgc tgttcttcat cctgtttaac ctggtgcgtc
    21301 tggcaacccg ttactacggc gtagcgtatg gttactccaa aggtatcgat atcgttaaag
    21361 atatgggtgg tggcttcctg caaaaactga cggaaggggc gtctatcctc ggcctgtttg
    21421 tcatgggggc attggttaac aagtggacac atgtcaacat cccgctggtg gtttcgacca
    21481 tcaccgggca ggatggacag acccgtgtca ccaccgtgca aactattctt gaccagttaa
    21541 tgccaggcct tgtaccactg ctgctgacat ttgcttgtat gtggctgctg cgcaaaaaag
    21601 ttaacccgct gtggatcatc gttggcttct tcgtcatcgg tatcgctggt tacgcttgcg
    21661 gcctgctggg actgtaagac tgttgtacac taccggggcc ttttggcccc gtttttttat
    21721 ctggaggatt aatgacaatc acggacctgg tactgattct tttcatcgcc gcactcctgg
    21781 ccttcgcgat ctacgatcag ttcatcatgc cccgccgtaa cggccccacc ctgctggcaa
    21841 ttcctttgct ccggcgtggt cgcatcgata gcgttatctt cgtcggattg attgtcattc
    21901 ttatctataa caacgtcacg aatcatggcg cgctaataac aacatggtta ttaagtgcac
    21961 tggctctgat gggtttttat atattctgga tccgcgttcc gaagatcatc tttaaacaaa
    22021 aaggtttttt cttcgccaat gtctggattg aatatagccg aatcaaagcg atgaacttgt
    22081 cggaagatgg cgtgttggtg atgcaattag aacagcgtcg gctgttaatc cgcgttcgaa
    22141 atatcgacga tctggaaaaa atttataagc ttctcg
//
LOCUS       DADLSE010000055        21738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLSE010000055 DADLSE010000000
VERSION     DADLSE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.055"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..798)
                     /locus_tag="JN692_002127"
     CDS             complement(<1..798)
                     /locus_tag="JN692_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7264439.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHG
                     AQLLDRLLEPGDIVGFSWGRAVSALVENLPQAGQSQQLICVPIIGGPSGKLESRYHVN
                     TLTYSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSP
                     AIRDGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEK"
     gene            1146..2018
                     /gene="rluF"
                     /locus_tag="JN692_002128"
     CDS             1146..2018
                     /gene="rluF"
                     /locus_tag="JN692_002128"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7264440.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2151..2423)
                     /locus_tag="JN692_002129"
     CDS             complement(2151..2423)
                     /locus_tag="JN692_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7264441.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLNNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN692_002130"
     CDS             complement(2676..4025)
                     /gene="lysC"
                     /locus_tag="JN692_002130"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7264442.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQAVQKLHSNLFE"
     gene            4550..6199
                     /gene="pgi"
                     /locus_tag="JN692_002131"
     CDS             4550..6199
                     /gene="pgi"
                     /locus_tag="JN692_002131"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7264443.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQGLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            6661..6903
                     /locus_tag="JN692_002132"
     CDS             6661..6903
                     /locus_tag="JN692_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264444.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            7017..7655
                     /locus_tag="JN692_002133"
     CDS             7017..7655
                     /locus_tag="JN692_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7264445.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            7652..8389
                     /locus_tag="JN692_002134"
     CDS             7652..8389
                     /locus_tag="JN692_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7264446.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRVPQ"
     gene            8389..10485
                     /locus_tag="JN692_002135"
                     /pseudo
     CDS             8389..10485
                     /locus_tag="JN692_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
     gene            complement(10532..10810)
                     /locus_tag="JN692_002136"
     CDS             complement(10532..10810)
                     /locus_tag="JN692_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264447.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            10811..10954
                     /locus_tag="JN692_002137"
     CDS             10811..10954
                     /locus_tag="JN692_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264448.1"
                     /translation="MFQRIIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            11080..11490
                     /gene="psiE"
                     /locus_tag="JN692_002138"
     CDS             11080..11490
                     /gene="psiE"
                     /locus_tag="JN692_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HAY7264449.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN692_002139"
     CDS             complement(11534..13009)
                     /gene="xylE"
                     /locus_tag="JN692_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HAY7264450.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN692_002140"
     CDS             complement(13381..14271)
                     /gene="malG"
                     /locus_tag="JN692_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7264451.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN692_002141"
     CDS             complement(14286..15830)
                     /gene="malF"
                     /locus_tag="JN692_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY7264452.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN692_002142"
     CDS             complement(15984..17174)
                     /gene="malE"
                     /locus_tag="JN692_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7264453.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            17539..18654
                     /gene="malK"
                     /locus_tag="JN692_002143"
     CDS             17539..18654
                     /gene="malK"
                     /locus_tag="JN692_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7264454.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            18726..20066
                     /gene="lamB"
                     /locus_tag="JN692_002144"
     CDS             18726..20066
                     /gene="lamB"
                     /locus_tag="JN692_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7264455.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            20218..21138
                     /gene="malM"
                     /locus_tag="JN692_002145"
     CDS             20218..21138
                     /gene="malM"
                     /locus_tag="JN692_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7264456.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPSLGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            21367..>21738
                     /locus_tag="JN692_002146"
     CDS             21367..>21738
                     /locus_tag="JN692_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264457.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMLREVSISNDTISVKFYRNEKIECACNFLMDKDAQG
                     YIDLSDFDLTNCHFKGDVISKV"
BASE COUNT         5455 a         5539 c         5587 g         5157 t
ORIGIN      
        1 tttttcgctc atattcgttt caaccattgc gccgtgaata tcaaaaaagc gcgagcaaat
       61 atcgccagca acctggcggg cattcaggtc gtcactctct tcaccaccat aaaacgcatg
      121 ccagttagcg ccgtcgcgaa tggccggtga gccaattccc accagggcga catccagatt
      181 atcccagtag gcagagatgg ttttaaagtg ctgagactgc atgatcccat tacgaattaa
      241 tgggttatcc agcagagccg gaaaatccgc gagatgcgat tcccctttca gcttcgctgc
      301 cgcgctgtag gttaatgtgt ttacatgata gcggctttcg agtttaccgg acggtccgcc
      361 aataattggt acgcagatta actgctgcga ttgccccgcc tgcggcaagt tttcaaccag
      421 tgcgctcacc gcgcgacccc aggaaaaacc gacaatatcg ccaggctcca gcaagcgatc
      481 cagcagttgc gcgccgtgta accccatcat cgccagttga gtctcttcat cctcatcatt
      541 tcccgacacc accacaacgt ctttcaggcc aaacttttgc ttcagttgct gctccagcca
      601 gagattttcg ttgtagtcat agtagtcata gttgatggcg atggtgacca ttccctgatc
      661 gcggcctcgt ttaagcaagc ggctgatggt ggtgcggtaa atacccagtt cgcgcgcgat
      721 ttgcgcctgc gtcatatcct gttcgtaata gagttgggca atcttcacaa tcaaacggat
      781 atcgtcactg ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct
      841 caccgcttga tctgatataa ccccatcatg agggcctttc aaagcgattt ttcactcttt
      901 gcatggggag aaatagcgct ttctcttgca catttgtttc tctgcgtagt agtcatcagc
      961 acaggtgtgc agcgattcgt aatatgttga ttatccttat tttgccaggc aaatttgcat
     1021 cgtacttccg gagacacttt tgtgataaaa ggcgtggcag atcacattta atttcgacgt
     1081 tgccgcataa ccctgttttt ccccgctaag aggtacggta tattccgcgt tttacaggag
     1141 aatttatgct gcccgactca tcagtccgtt taaataaata catcagcgaa agcggaattt
     1201 gctcacgccg cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc
     1261 gagccaccat tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac ggtcagttga
     1321 ttgagcctcg ggaagccgaa gatttggtac ttatcgccct gaacaagccc gtcggtattg
     1381 tcagcaccac cgaagatggc gagcgcgata acattgtcga tttcgttaac cacagcaaac
     1441 gcgtgttccc gattggccgc ctggataaag actcccaggg gctgattttc ctcaccaatc
     1501 acggcgatct ggtgaataag atcctccgtg cgggtaacga tcatgagaaa gagtatctgg
     1561 tgacggtcga taaaccgatt accgatgaat ttattcgtgg tatgggggcg ggggtgccga
     1621 tcctcgggac ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt gtcttccgca
     1681 ttaccctagt gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat ttcggctatg
     1741 aagtgaaaaa gctggaacgt acgcgcatta tgaacgtcag cttaagcggc attccgctgg
     1801 gggagtggcg cgatttaacc gacgatgagt taatcgacct ctttaagctc atcgaaaatt
     1861 cctcttccga ggcaaaacct aaagcgaagg ccaaaccgaa aacagcgggc atcaaacgtc
     1921 cagtcgttaa gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc aacggtaagc
     1981 gttttacctc gccggggcgt aaaaagaagg gacgctgatt aacgcctcag actgatgaaa
     2041 acgtccaaaa agagctcgga cagtcccctc gccccttcgg ggagagggtt agggtgaggg
     2101 gaagattgtg tccacacaga cgagtgaaaa tctcaggcgt tgattgttca ttagcgcctg
     2161 ccgcgcgtcg acgtatttgc tgaccagcta aatgacgctt caggatccgg tttataagcc
     2221 tgctttttct tcaattggcg ggcttttttc gcttcagcct cacgctcgac cattaattta
     2281 tcgatgtact ctttcttaat gctgttggtt tcggagttgt tcagtacgcg accatgcgca
     2341 atccgcgccc gatccagcag tgttttaagt tcacgctgct cgcgttcagt catctctttt
     2401 tgggtaatac gggggagtgc catgatggtg ccctcagtga gccagagagt tcagtgtacg
     2461 ggaattcgtt tgcgagcaga actgataata ttcattttgc cttctcttaa ctatttatct
     2521 ctttcgataa ttaattagag atgcagataa aaaaattaaa ggcaattatt ctccgataaa
     2581 gacgttggtt aacagagtac aggctcgttt attgatgagc atagtgacaa gaaaatcaat
     2641 acggcccgaa atatagcttc caggccatac agtatttact caaacaaatt actatgcagt
     2701 ttttgcaccg cctgctcggc atcttcgccg ggcaccagga agcacaggtt atggctggat
     2761 gcgccgtaac aaatcatgcg aatgttgaac ggttccagta cgccgaatac ctctttgcca
     2821 acgccgcagg cttttgacag gtcattgcca atcaacgcga ccagcgccag accttcttcc
     2881 acctccaccc ggcacagtgc ggaaagctcc atcagcagag attgcgtcag caacgtatcg
     2941 ccagtggagg ttgaaccggt ggtatcaagg gttaatgcca cgctcacttc tgacgtggtg
     3001 attaagtcta ccgaaatatt atgccgcgcg aggatgccga aaacttccgc gaggaagccg
     3061 cgagaatgca gcatattcag gctgtgcaaa gtgagcagag tctgattgcg acgaagcgcc
     3121 agcgcgcgga acagcggcgg attttcagtt ttattgcaca ccagcgtacc acctgcgcgt
     3181 gggtctttgc tggagccgac aaagaccggg atatcgctgc gtactgcggg tagcaacgtt
     3241 gccggatgca gtacttttgc accaaaagtt gccatctctg ccgcttcggc aaacgcgatt
     3301 tcatcaatgc gttttgctgc ggaaactacg cgtggatcgg tggtgtagat gcccgggaca
     3361 tcggtccaga tatcaacacg agatgcgtgt aaagcctccg ccagcaaggc tgccgtataa
     3421 tcgctgcctc cacggccaag cgtcgttgta cgacctttat tttcgctacc gataaatccc
     3481 tgggtgatca ctaagccttc attgaggcgt gggagcagct gcagcgcggc cagttccgcc
     3541 agcgcggcta catctggctc tgcacgacca aatcggtcgt tggtacgcat cactttacgt
     3601 acatcaaacc actgtgcctg aacatcgcgt tcgcgcagga tttcgacaaa cagcagggtc
     3661 gacatcagct cgccgtggct gaccagctca tctgtcagcg ccggagacgt tgccagcgcc
     3721 gccgcttctg ccagaacagt aatgttctcc agcagacgtt caatctcttc acggataacg
     3781 ttcgggtaac gcagacgttc cagaatggca aactggatgt tgcgaatagc gtcgagtttt
     3841 tcgaatcgct cgccaggttc cagcccttcc gctaaagcga ccagcagatt agtgatacca
     3901 gcagaagccg agaggactac taaacgcacg ttggcatcag aaagcacaat atcagcgctg
     3961 cggttcatgg cgtcaaaatc agctacgctg gtaccgccaa atttggagac aacaatttca
     4021 gacataacta cctcgtgtca ggggatccat tttcagcctt ggcacaaggg aagagcggaa
     4081 gacgggtggg cgcagagcga tacttcgcta ctattttcac ccagaagtgc tccaccactt
     4141 gcgaaacgcc cgactgcgaa cgcttctggt gacaacccag gggattcagc ccctgtagcc
     4201 gatgatgaac gtggccagcc gttcaatcac ctcggcgatg caccccctca ggtgttatca
     4261 caggactggc tcctccaaca ccgttacttg ggcaacgcgc ctcttctggc ctgcgctagc
     4321 gcaggtagta catttataaa taaagggtga gcggggcggt tgtcaacgac ggggtcatgc
     4381 ggatttttca tccactcctg gcggtcagta gttcagctaa taaatgcttc actgcgctaa
     4441 gggtttacac tcaacattac actaacggca ctaaaaccat cacatttttc tgtgactggc
     4501 gctacaatct tccaaagtca caattctcaa aatcagaaga gtattgctaa tgaaaaacat
     4561 caatccaacg cagaccgctg cctggcaggc actacagaaa cacttcgatg aaatgaaaga
     4621 cgttacgatc gccgatcttt ttgctaaaga cggcgatcgt ttttctaagt tctccgcaac
     4681 cttcgacgat cagatgctgg tggattactc caaaaaccgc atcactgaag agacgctggc
     4741 gaaattacag ggtctggcga aagagtgcga tctggcgggc gcgattaagt cgatgttctc
     4801 tggcgagaag atcaaccgca ctgaaaaccg cgccgtgctg cacgtagcgc tgcgtaatcg
     4861 tagcaatacc ccgattctgg ttgatggcaa agatgttatg ccagaagtca acgcggtgct
     4921 ggagaagatg aaaaccttct cggaagcgat tatctccggt gagtggaagg gttataccgg
     4981 caaagcaatc actgacgtag tgaacatcgg gatcggcggt tctgacctcg gcccgtacat
     5041 ggtgaccgaa gcactgcgtc cgtacaaaaa ccacctgaac atgcactttg tttctaacgt
     5101 cgatgggact cacatcgcgg aagtgctgaa aaaagtaaac ccggaaacca cgctgttcct
     5161 ggtagcatct aaaactttca ccactcagga aaccatgacc aacgcccata gcgcgcgtga
     5221 ctggttcctg aaagcggcag gtgatgaaaa acacgttgcg aaacattttg cggcgctctc
     5281 aaccaatgcc aaagccgttg gcgagtttgg tattgatact gccaacatgt tcgagttctg
     5341 ggactgggtc ggcggccgtt actctttgtg gtcagcgatt ggcctgtcga ttgttctctc
     5401 catcggcttt gataacttcg ttgaactgct ttctggcgca cacgcaatgg acaagcattt
     5461 ctccaccacg cctgccgaga aaaacctgcc tgtcctgctg gcgctgattg gcatctggta
     5521 caacaatttc tttggtgcgg aaactgaagc gattctgccg tatgaccagt atatgcaccg
     5581 tttcgcggcg tacttccagc agggcaatat ggagtccaac ggtaagtatg ttgaccgtaa
     5641 cggtaacgtt gtggattacc agactggccc gattatctgg ggtgaaccag gcactaacgg
     5701 tcagcacgcg ttctaccagc tgatccacca gggaaccaaa atggtaccgt gcgatttcat
     5761 cgccccggct atcacccata acccgctctc tgatcaccac cagaaactgc tgtctaactt
     5821 cttcgcccag accgaagcgc tggcgtttgg taaatcccgc gaagtggttg agcaggaata
     5881 tcgcgatcag ggtaaagatc cggcaacgct tgactacgtg gtgccgttca aagtgttcga
     5941 aggtaaccgc ccgaccaact ccatcctgct gcgcgaaatc actccgttca gcctgggtgc
     6001 gttgattgcg ctgtatgagc acaaaatctt tactcagggc gtgatcctga acatcttcac
     6061 cttcgaccag tggggcgtgg agctgggtaa acaactggca aaccgtattc tgccagagct
     6121 gaaagatgat aaagaaatca gcagccacga tagctcgacc aatggtctga ttaaccgcta
     6181 taaagcgtgg cgcggttaat catcatgcgt gcctgatgcc gatatgtagg ccggataagg
     6241 cgtttacgcc gcatccggca accggagcct gatgccaata tcgacattaa tgcatagtat
     6301 gaggagttaa ataaccttaa aaacaatgcc gacaataagt cggcattgtt ttatcagata
     6361 aatccccttg tctgtgattt aacggaaatc ataccgtgag gttaatccta aaatagattt
     6421 ttaatcgttg tttatttcgg agaatacgca gattaattac ttttgttttt attttaagtt
     6481 tatgattttt attgttattt aaatataagt tgaaacttat atttgatatt cattccaatt
     6541 atcctaaaac tccatcgcta attccccgcg ccgtaattcg catgctttag ttgtgtatac
     6601 tcgatcccgc ccgaaatgtt tttgggtaaa tctccattca ttcaatgaag ggaaattgtt
     6661 atgaaaaaag ttctgtatgg catttttgcc atatctgcgc ttgcggcgac ttctgcgtgg
     6721 gctgcacctg tacaggtggg cgaagcggca gagtcggcag caacgtcggt ttcggcgggg
     6781 agttcctccg cgaccagcgt cagcaccgta agctcggcgg tgggtgtcgc gctcgcggca
     6841 accggtggcg gtgatggttc taataccggg accaccacaa ccacgaccac cagtacccag
     6901 taataaagta tgtatcccca aaataatttg agtcattgca tctgtggctt gaagtatgaa
     6961 gggatttacc ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga
     7021 agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc acgactaaag
     7081 agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc aggcgtacag ctgacggacg
     7141 atgatattca aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcaat
     7201 tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcaag
     7261 ccactcttgt cacacaacat ggtcgtctgg tgaagacttt gcttggcggc gacaacctga
     7321 ttgaagtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatt gttgatggtg
     7381 caacatggac gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcac
     7441 gctcagtctt caaatgggat ggcaccgata ccgtcaaggt cggcagcgat gaaaccctgg
     7501 ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac cgctactgga
     7561 tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat tatttcccgg
     7621 tgaaaaccac tctcatcaag gcggcaaaac aatgattaaa caaactattg tcgcgttgct
     7681 tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt tcagcaatgg
     7741 cagcagtgag gccaaaacgc taacgggcgc agagcattta atcgatctgg tagggcaacc
     7801 tcgactggca aacagctggt ggcctggtgc ggtgattagc gaagagctgg caacggcggc
     7861 agcattgcgt cagcagcagg cgttgctgac acggctggca gaacagggcg cagattccag
     7921 caccgacgat gccgccgcca ttaacgcctt acgccagcaa atccaggcat tgaaagtgac
     7981 aggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgccgaac gcggtaaccc
     8041 gccgttgcag ggcaactaca cgctgtgggt cggaccaccg ccgtccacgg tcacgttgtt
     8101 cgggcttatc agccatcctg gcaatcagcc attcactcca ggtcgcgacg tggcgagcta
     8161 tctctctgac cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg tcgtttaccc
     8221 ggacggacgc acgcaaaaag cgccggtggc ttactggaac aagcgtcacg tagagccgat
     8281 gcccggcagc attatttatg ttggcctcgc agactccgtc tggagtgaga cgcctgatgc
     8341 ccttaacgcc gacattcttc agactctgac gcagcgggta ccccaataat gaaaaaaaga
     8401 catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga aacatatcct
     8461 gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca aactcccacc
     8521 gcgcgtatgg cacgggaagg ggagttgagt ctgaactatc gcgataacga tcagtaccgt
     8581 tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg ctacaccgac
     8641 gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat
     8701 aaagccttcg atctcaaact gcgtttgtgg gaagagagtt actggctgcc gcaagtggcg
     8761 gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc
     8821 aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatatct gggcaccagc
     8881 ggtaatgtaa aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc
     8941 tacaaacagg cgggatctat cgacggtagc cagatgttcc acggtcctgc ctcactgttt
     9001 ggcggcgtgg aataccagac gccttggcaa ccgctgcgtc tgaaactgga gtatgaaggt
     9061 aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg
     9121 gcgatttatc gcgttatcga ttgggccgac gttaacctta gctatgaacg tggcaacacc
     9181 tttatgtttg gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc ctacatcgat
     9241 aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca ttcggtggtg
     9301 gcgaatcagt taacgttgca gaaatacaac gccggacttg ccgatccgca gatccaggcg
     9361 aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc gcgcgaaggg
     9421 atcatccgtg ctaatcggat cgtgatgaac gatctgccgg atgggatcaa aacgatccgc
     9481 attacggaaa atcgccttaa catgccgcaa gtgacgacgg aaaccgatgt cgccagcctg
     9541 aaaaatcatc tcgccggaga gccgttgggc cacgaaacga cgctggcgca caaacgcgtc
     9601 gagccagtgg ttccgaagtc caccgagcag ggctggtata tcgacaaatc acgctttgat
     9661 ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat
     9721 cagctgggcg tgatgggaac ggcagatttg tggctgacgg accatctact gaccaccggc
     9781 agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa tccaccgcag
     9841 gactcgcact taccgcgcgt tcgtacccat gtgcgcgagt atgtgcagaa cgatgtctat
     9901 gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc
     9961 tatggaggtt atctcgaaac catgtttggc ggtgcggggg ccgaagtgtt gtatcgtccg
    10021 ctggacagta actgggcgtt tggtctggat gccaactacg ttaaatagcg cgactggcgt
    10081 agtgcaaaag atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcc
    10141 tactggacgc catctttcgc ccaggacgtg ttggtgaaag ccagcgtcgg gcagtatctg
    10201 gcaggggata aaggcggcac gctggagatc gctaaacgct ttgacagcgg cgtggtggtg
    10261 ggtggctatg ccacgatcac caacgtttcg aaagaggagt acggcgaagg ggacttcacc
    10321 aaaggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac acgcagccgt
    10381 gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc
    10441 cagttgtatg acatgaccag cgaccgtagc gtaaatttcc gctaagtcat gggaaaggtg
    10501 ccagttttcg cactcactgg caccctcatt cttaatgacc tgttgagcca tcaggaacgc
    10561 agttcacccc cggtttacga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg
    10621 tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg ctggagacat
    10681 tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa
    10741 cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt
    10801 tacgtttcat ttgttccaga ggatcattgt cgatttttcg cgcattgctg gtggctggga
    10861 atcacctgaa tgggtgattt ttgaattacc ggctttggtg cggtttgtct tgccggatgc
    10921 gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg tggaggcgcg
    10981 ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc acatttttgc
    11041 gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact ctctcgtccg
    11101 cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct gctgtgcctc
    11161 ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggctga tgtgctgttc
    11221 gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta ctttctctat
    11281 ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt cccgctacgt
    11341 tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga tcataaatcg
    11401 ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac cctgtggctg
    11461 tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaaatg gcgcggcgtg
    11521 ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc ggttcccaga
    11581 gcgcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc cacataaaca
    11641 gtgctgccag aacgcccata caaccgtaaa tccagtagga gaaaccgttg tggaaatggg
    11701 ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag tagttcgcca
    11761 gccactgggc cgccaccgcg attgccagcg ctttaccacg aatagcattc gggaagattt
    11821 ccgacagcag tacccagcat accggacccc aggacatggc aaaggcggca acatagaaca
    11881 gcatcgacag tagcgccaca atacccgatg cctgagtgta aaacgcggta ccgaggctaa
    11941 acataccgat tgccattccg agtgcgccga taatttgcag tggcttacga ccaaatttat
    12001 ccacagtcat aattgccagt acggtaaagg tgaggttgat aactccgaca ataatggtct
    12061 gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc gcgtagtaca
    12121 gcaccacatt gatgccgaca aattgctgga agatggagag cataacgccg attacaatca
    12181 cgcccacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc agggagtgtt
    12241 taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc aggatacttt
    12301 ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc acggtataca
    12361 gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa cgccagccgt
    12421 cagtattcag ccagctggca tcaccggaac gggcaataaa atagtttacg cagtaaacta
    12481 aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagttttccg cgaatatgag
    12541 ctggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca acgccaatac
    12601 cgccaataat acgataaata acaaattccg ggacataacc tgccagataa ataggtactg
    12661 tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct acaccagaaa
    12721 taaaaaacag gacagcagaa atcttaagtg aatcacgacg gccgaagcgg ttactgcaat
    12781 aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggctacg caaaatccta
    12841 acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg gtattgagtg
    12901 actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca cctaatgtag
    12961 cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt cagacctgcc
    13021 ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc agttaaggca
    13081 attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa ttgtgataat
    13141 tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa gagaattatc
    13201 gtaagtggga aaacaaataa cgtaaaaata ataagttcta tgatgggaat attaaccggc
    13261 gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc gaataatagt
    13321 cacaagttgg gataacgtaa gttgagggtg cagcggcata acattggcag aacaacatct
    13381 ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag ccagcaagaa
    13441 gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac cccacaggta
    13501 gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt ttacgtcacg
    13561 cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca ggatgaatac
    13621 caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct gccacggtgt
    13681 cgcaccatcc agcgcagccg cttcttccag cgaactgtcg atggtttcga aatagccttt
    13741 gatggtccag acatgcagcg caatcccacc cagatacgcg aaaattacgc cgccgtgagt
    13801 attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat acaacgcgac
    13861 cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca gcgtcgcttt
    13921 gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg ccacaatgcc
    13981 aatcgcggaa atcccagcga cctttaccga gttccacagc cacagcagta ccgggaatgg
    14041 cggtggcgta atgcgaccat cagcctgttc aacgctaaaa cctaacgcca gtttccagtg
    14101 atcccaggag atttgctccg ggatcaggct accggtcgcg aagtttccct gacgcagcga
    14161 gatagcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta gcagtagcag
    14221 gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca tttttgttat
    14281 ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag cgcacccacc
    14341 agcaggaaga tcagcgtggc aattgctgcc gccagaccga agtcctgacc cccgccgcct
    14401 tcaaaagcga tacggtaggt gtagttaaca agcaggtcgg tataaccggc tggcgtggtc
    14461 gtgccaagac gatccgggcc gccgttggtt aacagctgaa tcagcacgaa gttgttaaag
    14521 ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag cggcagcgta
    14581 atcttaaaga agttctggaa cggacctgcg ccatccattg ctgaggcttc atacaaatcg
    14641 tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata acccagccag
    14701 gtattgacga taatcagcat cgtgcgagcg gtggtcggat cgctgaacca ggcgggcttc
    14761 acgccaaaca gcgcgctcaa catcatgttg atttcaccga agctctggtt aaacaatcct
    14821 ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag caggacgcga
    14881 tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac atgccagaac catgccgacc
    14941 gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa aatggcgagg
    15001 aacggtttct gaatgccttc gtcggtaaag acgcgggtaa agtttttcca gccggtggtc
    15061 acggtgtaac cggggcttag cttttcatcc ccccagttgc cgtcggcggt aatggactgg
    15121 taaaagccaa tttggttatt cggacgatat ttcacgccac tctgattatt cgtcaacgtg
    15181 ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag ggagctcatc
    15241 atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg acgattctgg
    15301 gtaatcacgc gcagattcgc gcgttcgcct tcgggctggg cggtcgtttc tttcagttgc
    15361 agtttttgct cgccgccaaa tttaaacgcg tcggagaggt aatttttgcc ggtttcgccg
    15421 tcgctgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt ataggttttg
    15481 cctgcttgcc aggagcgatc taacaacact tcctgcgcac gttcaaaagt cagctggtta
    15541 gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa gaggacgaat
    15601 aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt acgattggcg
    15661 aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag gtattcccct
    15721 tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc tagcactgac
    15781 catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat ggggttcttc
    15841 ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc tgatgcgaca
    15901 ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt aggccggaca aggcgttcac
    15961 gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc tttcagggct
    16021 tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgtac ggcataccag
    16081 aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt ttccatggtg
    16141 gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc tacggcaccc
    16201 agcggtttgt ctttattaac cgcttccaga ccttcatcag tcagcagata gttttcgagg
    16261 aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct cagcacgcca
    16321 acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc ataattcact
    16381 ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc tgtttcgcct
    16441 ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg tttgttttta
    16501 atcaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac gcccacgtct
    16561 ttaatgtcgt acttgccgtt ttcatactta aacgcataac ccccgtcagc agcaatcagc
    16621 ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc tttcgctttc
    16681 agttctttat ccagcgccgg gatctcttcc caggtttttg gcgggttcgg cagcagatct
    16741 ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag cttgccgttg
    16801 taacgtacgg catcccatgt aaacggatac agcttgtcct ggaacgcttt gtccggggtg
    16861 atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgcgc ccagaagata
    16921 atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag tttatccgga
    16981 tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac ttcagcgaga
    17041 ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc gattttggcg
    17101 agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat gcgtgcacct
    17161 gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa cacctaaacg
    17221 gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac gccccaccgt
    17281 tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gggatgcgca cataaaatcg
    17341 ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt agttcacaga
    17401 agccgcgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt ggaggattta
    17461 agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga tgacaggttg
    17521 ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa agcctggggc
    17581 gaggtcgtgg tatcgaaaga tatcaatctc gatatccatg aaggtgaatt cgtggtgttt
    17641 gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg gcttgagacg
    17701 atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc gccagcagaa
    17761 cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc agtagcagaa
    17821 aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa ccaacgcgtc
    17881 aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc gaaagcgctc
    17941 tccggtggtc agcgtcagcg cgtggcgatt ggccgtacgc tggtggccga gccaagcgta
    18001 tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca aatgcgtatc
    18061 gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac ccacgatcag
    18121 gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg cgtggcacag
    18181 gttgggaaac cgctggaact gtaccactat ccggcagacc gttttgtcgc cggatttatc
    18241 ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat cgatcaagtg
    18301 caggtggagc tgccgatgcc aaatcgtcaa caagtctggc tgcctgttga aagccgtgat
    18361 gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact gccgagtgat
    18421 atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg caacgaaacc
    18481 caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca gaacgacgtg
    18541 gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg ttgccatctg
    18601 ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt ttaagcaccc
    18661 cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat gactcaggag
    18721 atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca gcgggcgtaa
    18781 tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt attggctgga
    18841 caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt aaataccgtc
    18901 ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg tggaaagagg
    18961 gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa cagaatgact
    19021 gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggaaaaaac ctgatcgaat
    19081 ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat gacgttcata
    19141 tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa aacatcgatg
    19201 ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt ggttcttcct
    19261 cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac gttttcgatg
    19321 tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt gtcgactacg
    19381 gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa gacggctggt
    19441 tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt gttgttcagt
    19501 acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct ggcgtcgcgt
    19561 ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg cgtatcctcg
    19621 accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt atgtaccagg
    19681 atatcaactg ggataacgac aacggcacca agtggtggac cgttggtatt cgcccgatgt
    19741 acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac gtcgaatccc
    19801 agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa tggcaggctg
    19861 gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc aagtgggatg
    19921 agaaatgggg ttacgactac aacggcgata gcaaggttaa cccgaactac ggcaaagccg
    19981 ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag tggaccttcg
    20041 gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg cgtttacgcc
    20101 gcatccggca taaaaacagg ttgtcattat ctgaaagggg cgaaagcccc tctgattatc
    20161 gggtttagcg cgctattgcc tggctaccgc tgagctccag attttgaggt gaaaacaatg
    20221 aaaatgaata aaagtctcat cgccctctgt ttatcagcag ggttactggc aagcgcgcct
    20281 ggaattagcc ttgccgatgt taactacgta ccgcaaaaca ccagcgacgc gccagccatt
    20341 ccatctgccg cgctgcaaca actcacctgg acaccggtcg atcaatctaa aacccagacc
    20401 acccaactgg cgaccggcgg ccaacaactg aacgttcccg gcatcagtgg tccggttgct
    20461 gcgtacagcg tcccggcaaa cattggcgaa ctgaccctga cgctgaccag cgaagtgaac
    20521 aaacaaacca gcgtttttgc gccgaacgtg ctgattcttg atcagaacat gaccccatca
    20581 gccttcttcc ccagcagtta tttcacctac caggaaccag gcgtgatgag tgcagatcgg
    20641 ctggaaggcg ttatgcgcct gacaccgtcg ttggggcagc aaaaacttta tgttctggtc
    20701 tttaccacgg aaaaagatct ccagcagacg acccaactgc tcgacccggc taaagcctat
    20761 gccaaaggcg tcggtaactc gatcccggat attcccgatc cggttgctcg tcataccagc
    20821 gatggcttac tgaaactgaa agtcaaaacg aactccagct ccagcgtgtt ggtaggacca
    20881 ctatttggtt cttccgctcc agctccggtt acggtaggca acacggcggc accagctgtg
    20941 gctgcacctg ctccggtgcc ggtgaagaaa agcgagccga tgctcaacga cacggaaagt
    21001 tattttaata ccgcgatcaa aaacgctgtc gcgaaaggtg atgttgataa ggcgttaaaa
    21061 ctgcttgatg aagctgaacg cctgggatcg acatctgccc gttccacctt tatcagcagt
    21121 gtaaaaggca aggggtaatt acgccccaca gtgctgattt tgcaacaact ggtgcgtctc
    21181 ctggcgcacc tttttttatg cttccttcct gggatatgag cgatttttta tagtacttca
    21241 cttcttcttc actaagaatg tccattattt caatgcatta tcagagattc ttttcctttc
    21301 gccggtagtg tctggacgct caggctactt ttccaggtta ttttatttct gtattacaga
    21361 ggttttatga taggtcatat cacaaattct ggcggcaata actctttgat gaaacatgat
    21421 gtggtgcaag gaaataatat agtagatctt gatttactac gtaatttcaa tggggtgaca
    21481 ggtttaaaca gagataactt tatttatatc agtgatatat ttttaaatat aaaacaacgg
    21541 aacgaaaaaa atcatgcaat aaatatgtta cgtgaagttt caattagtaa tgatactata
    21601 agtgtaaaat tctacagaaa tgaaaaaata gaatgcgctt gtaattttct gatggataaa
    21661 gatgcgcagg ggtatatcga cctgtctgat tttgatttaa caaattgtca ttttaaaggt
    21721 gacgttattt ctaaggtg
//
LOCUS       DADLSE010000056        21686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLSE010000056 DADLSE010000000
VERSION     DADLSE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.056"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN692_002147"
     CDS             complement(15..1010)
                     /gene="yjfF"
                     /locus_tag="JN692_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7264458.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(997..2019)
                     /locus_tag="JN692_002148"
     CDS             complement(997..2019)
                     /locus_tag="JN692_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7264459.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2033..3535)
                     /locus_tag="JN692_002149"
     CDS             complement(2033..3535)
                     /locus_tag="JN692_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7264460.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQFGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN692_002150"
     CDS             complement(3675..4631)
                     /gene="ytfQ"
                     /locus_tag="JN692_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HAY7264461.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            4941..5471
                     /gene="ppa"
                     /locus_tag="JN692_002151"
     CDS             4941..5471
                     /gene="ppa"
                     /locus_tag="JN692_002151"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7264462.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5851..6192)
                     /locus_tag="JN692_002152"
     CDS             complement(5851..6192)
                     /locus_tag="JN692_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7264463.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN692_002153"
     CDS             complement(6195..9974)
                     /gene="tamB"
                     /locus_tag="JN692_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7264464.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN692_002154"
     CDS             complement(9971..11704)
                     /gene="tamA"
                     /locus_tag="JN692_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7264465.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11910..12539
                     /gene="msrA"
                     /locus_tag="JN692_002155"
     CDS             11910..12539
                     /gene="msrA"
                     /locus_tag="JN692_002155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7264466.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGVCLPPEA"
     gene            12862..14205
                     /locus_tag="JN692_002156"
     CDS             12862..14205
                     /locus_tag="JN692_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7264467.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14267..14473)
                     /locus_tag="JN692_002157"
     CDS             complement(14267..14473)
                     /locus_tag="JN692_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7264468.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14798..15352
                     /locus_tag="JN692_002158"
     CDS             14798..15352
                     /locus_tag="JN692_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7264469.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15415..16353)
                     /locus_tag="JN692_002159"
     CDS             complement(15415..16353)
                     /locus_tag="JN692_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264470.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN692_002160"
     CDS             complement(16565..17305)
                     /gene="cysQ"
                     /locus_tag="JN692_002160"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7264471.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17495..19433
                     /gene="cpdB"
                     /locus_tag="JN692_002161"
                     /pseudo
     CDS             17495..19433
                     /gene="cpdB"
                     /locus_tag="JN692_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
     gene            complement(19559..19939)
                     /locus_tag="JN692_002162"
     CDS             complement(19559..19939)
                     /locus_tag="JN692_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7264472.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYSVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20028..20888
                     /gene="qorB"
                     /locus_tag="JN692_002163"
     CDS             20028..20888
                     /gene="qorB"
                     /locus_tag="JN692_002163"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7264473.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            20997..>21686
                     /locus_tag="JN692_002164"
     CDS             20997..>21686
                     /locus_tag="JN692_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7264474.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDCARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRP"
BASE COUNT         5224 a         5812 c         5340 g         5310 t
ORIGIN      
        1 acggagaggc atttttatcg ctgcgcaatg ttgactcttg tcactggcga actctgacga
       61 ttctcccaca gcaccgttaa cccacgctgt aatgctataa aaataaacaa caaaataccg
      121 atggcgattt tcgtccacca ggaactcagc gtgccgtcaa agttgatgta agtctggatc
      181 agtccctgaa ttgccacgcc aaaaagcgtc ccaagcaccg tcccgacgcc accacttaac
      241 aacgtaccgc caatcaccac cgacgcaatg gcgtccagtt ccacacctac gcccgccagt
      301 gcatatccgg cttgggtata aatcgagaag acaatccccg ccagtgttgc cagtccggtg
      361 gagagcatat aaatgcgaat agtggtgctg cgagtagaaa tccccatcag gttcgccgac
      421 gtcgcgttgc cgccaatagc gtacacctga ttgccaaaac gggtacgatg cgcaaggaaa
      481 ataccgataa ccaccaccgc cagcatcaac agccccatcg cactaaggcg accgccgccg
      541 gggattttcc acgcaaggct tgagagcgtg tcataaatgg gatggtttat cggaatcgac
      601 tcttccgaaa cgagatagct gacgccgcgc aaaaagaaca tccccgcaag cgtaatgata
      661 aatgccggga tcttcagggc gtcgatcaga agccccataa atgcgccgaa ggcacagccc
      721 atcaccagca ctagcggaaa cgccagcagc ggcgagaggc cgaaatcgcc aaccactttt
      781 gccagaaaca cgccagtaaa ggcgatcacc gaaccgacgg agagatcgat accgccagag
      841 aggatcacaa aggtcatgcc aacggcgatt atcccaagaa aggcgttatc ggtcaggata
      901 ttgcagatca cccttgtgga agcaaaaccg ggaaactggg tcaggcagta gagatagccc
      961 agcacaaaga cgccgatggt gatcatcagc ggcaagttac gttttatcat ggctacgtac
     1021 tcctttaatc agactgataa agcgttgcga ctgaacaatc agcacacaaa gcaccaccac
     1081 cgctttcacc acctggttca tctccggcgg aaagcccgaa agcaaaattc cggtgttcat
     1141 cccctgaata atcagcgccc ccactaccga gagcagcagg ttaaagcgcc cgcccatcag
     1201 cgatccgccg ccaatcacca ccgcgagaat ggcgtccagc tccagccata atccggcgtt
     1261 gttggcatcg gcaccgcgaa tatccgccgc cacgataatg cccgcaatcg ccgcacatag
     1321 cccgctcaac acgtaggtga gcatgacgat gattcgcgtg tttaccccgg cattttttgc
     1381 cgcccgaatg ttgataccaa cggcttcgat aaacatcccc agcgccgttt tgcgggtcaa
     1441 cagccagaac aggagaagcg tcagcaccgc gataatgacc ggcgttggca ggaacaacag
     1501 cgatccgctg ccaaaccatg agagatccgg cgagttaaac gtgacgatct gcccggcggt
     1561 gatcagttgc gccacgccgc gcccggcgac catcaggatc agggtggcga caaacggctg
     1621 aattttgagg atcgctacca gtatgccgtt ccacaatccc gccaggatgc cggtgcccag
     1681 ggcgcttaac aaaacaatcg gcaggctgaa tcccgcgacc gtcatcgcag ccgttgtggc
     1741 tccggcgata gccatcaccg cccccacaga gagatcgata ccaccggtgg cgatcaccag
     1801 cgtcatacca atcgccagta acgcaacggg agctgcacgg ttaagaatgt cgatagggct
     1861 accgaacaaa cgcccatcct gaagcaccac ctgccagaaa tgcggggcta ccaggctatc
     1921 gaccagcagc accagcaaca gtgccgccag ctgcggcatc cccgtcggcc agtgaaaacg
     1981 cctttttggc ggcgtagtgt cagggagaga ttgaggcatc acactgttct ccttacgccg
     2041 caatggcgtt catgatcgcc ggaacggaaa gctgtgccag cgggatctcc gccacctgtt
     2101 tgcgatcgcg catgatgatc acccggtcgg catagcccac cagctcttcc agttcggagg
     2161 agatcaccag cagcgccaga ccgtcagcac acagcgtttc aatcaggcgg atgatctcgg
     2221 cgtgggcacc aacatcaatg ccacgggttg gctcatcaag gatcagaaat tgcggtcggg
     2281 tcagtagcca acgtgaaagc agcacttttt gctgattgcc accggagaga aattcaatcg
     2341 gttgttcagt tgaaggtgtg cgaatgccaa actggcggat aaagcgttcg gcaatctctt
     2401 gctgctcttt gcgggaaatg ggtcgtagcc agccgcgctg ggcctggaga gcgaggatga
     2461 tattttcccg caccgaggcg gcggcgatga tgccatcggt tttcctgtct tccgggcaga
     2521 atccgatgcc cagtactgaa gcctgatgtg gcgatcgcag ggtttgcggt ttgcctttga
     2581 tcaacgccgt gccgctgtca gcaggtttga taccgaagat cacttcggcg gtttcggtac
     2641 gtccggatcc cagcaagcca gccagaccga cgatctcgcc ggggcgcact tcgagatcaa
     2701 acggtgctat cgttcctttt ttgccgtaat ttttgaacgc ggcaacgggt ttgtcgctca
     2761 acaatgttcg cccggcacgc tgtagcgcgt gggtatccag ctcgcgcccc agcatcattt
     2821 ttaccagttc gatctgcggt agctcgcgcg tttcccgaca gcctacgaaa ctgccgttgc
     2881 gtaagacggt gatccgatcg ctgacctgat agacctgatc gagaaagtga gtgacaaaga
     2941 tcaggctgac gccgcgatcg cgcaactgac gcatcaggcc aaataatagc tccacttcct
     3001 gggtgtcgag gctggcggtg ggttcatcga ggatcagcac tttggcggag agatcgatag
     3061 cccggcaaat ggcgacgatt tgctgcatcg ccaccgaaaa acggttaagc ggttcgcgca
     3121 cgtcgaggga gaaaccgtaa gatgccatca gttcggtggc gcgcttttcc atctctttac
     3181 ggcgtagaag gccgaaacgt ttgggttcgc gacctatgaa tagattatca gcaaccgaca
     3241 tattgggtag caggttgact tcctgataga cggtgccgat accgagttgt tgtgcatggg
     3301 cggtattttt cggtgagata gcctggcctt ccagccagat ggtgccgcga tcggcgtggt
     3361 atacaccagt taatgctttg attagcgttg attttcccgc cccgttttca ccaagcagcg
     3421 ccatgatttc gccacggcgc aggctgaaat caacgttgtc taacgctttg acgccgggga
     3481 aaaatttact taatccttcg gtgcggagga tctcctggtg ttggtcggtc gtcatggttt
     3541 cccccggtgt aaagcgtgct cccagtaggc atgataagac gcggtaagcg tcgcatcagg
     3601 cttcagtgca ccatcgccgg atgcggcgcg agcgccttat ccggcctacg aatcggcatc
     3661 aggcatagca accctcaata ccccatattt ttcttcttct ctaactcttc ttttgcggta
     3721 tcaggcaggt aaagtgtgga tttggttaac gtcagctttt caggcatggt gccgtctttt
     3781 ttgtatttct ccagcgcatc gaaagcgggt cccgccatgt tcggcgtcag ttcaacactg
     3841 gcgttcgctt cgccatccat catcgctttg tagatgtccg gtacaccgtc aatggaaccc
     3901 gtgaggatat ctttgcccgg tttcaggccc gcttctttaa ttgcctgaat tgcaccaatc
     3961 accatgtcgt cgttatgggc gtaaaccatg cagatgtttt tgccgttgtt ttccgctttg
     4021 ataaagctct ccatgacttc tttgccttta ctgcgggtga agtcacctga ctgcgagcgg
     4081 ataattttga tatttggcgc attcttaatg gcttcggcaa agcctttctt acggtcaatg
     4141 gcgacgctgg ccccaacggt gccctgcagc tccaccacgt tgcatggttt gccattcact
     4201 tcttttacca gccagtcacc gatcaactta ccttcgagga tgttgtcggc agtaacggtg
     4261 gtcatataga gagatttgtc tttcacatcg atggaacggt cgagcaagaa gaccgggatt
     4321 tcggcatctt tcgcctcttt taataccggt tcccaacccg tcgcaaccac tggagcgata
     4381 aagatcgcat ccaccccttg tgcaacgaag gaacgtaccg ctttaatctg gttttcctgc
     4441 ttttgctgac catcggcaat tttcaacgta attccgcgtt tttcggcctc acttttcgcc
     4501 acattggttt ctgcggcgcg ccagccggat tccgatccga cctgcgaaaa tcctacggtt
     4561 aatggagcgg ccaacgccat agacgacatg gctgccgaga ctgcagagac tacaagtaag
     4621 cgtttccaca tagggttgtc ctcgtcgggg taattattat tggtgaaaag atgttcgcga
     4681 aaaaactata gacaattcgt tatgtaacgg attgcgttac atcacacttc aaaaaagtaa
     4741 ataaaacgtt aatcacaagt ttgtaatcgc tttcatctca ctatgaaaaa tgcggctacg
     4801 gttatggatt ttcctgctct gtataccgtc ttaaaactgg cgaaaaagga aaatgaagac
     4861 gaaaacaagc aaagacattc ggcgcgagtt ggctataata ctcggcactt gtttgccaca
     4921 tatttttaaa ggaaacagac atgagcttac tcaacgtccc tgcgggtaaa gatctgccgg
     4981 aagacatcta cgttgttatt gagatcccgg ctaacgcaga tccgatcaaa tacgaaatcg
     5041 acaaagagag cggcgcactg ttcgttgacc gcttcatgtc caccgcgatg ttctatccgt
     5101 gcaactacgg ttacatcaac cacaccctgt ctctggacgg tgacccggtt gacgtactgg
     5161 tcccgactcc gtacccgctg cagcctggtt ctgtgatccg ttgccgtccg gttggcgttc
     5221 tgaaaatgac cgacgaagcc ggtgaagatg cgaaactggt tgcggttccg cacagcaagc
     5281 tgagcaaaga atacgatcac attaaagacg taaacgatct gcctgaactg ctgaaagcgc
     5341 aaatcgctca cttcttcgag cactacaaag acctcgaaaa aggcaagtgg gtgaaagttg
     5401 aaggttggga aaacgcagaa gccgctaaag ctgaaatcgt tgcttccttc gagcgcgcaa
     5461 agaataaata agttcttcta gcgcaataac cctgaacgcc gggcttcggt tagtaagggt
     5521 ttttttatgc ccgcgataaa taaactctct attccaccat cattattctc agcggttgca
     5581 aggcttgaac ggtaagaaca agcaaacccg accaccattt tgctgttcat agccacttgc
     5641 tggaagttag ccgacctcac tcatactcac cgttcaaagc gtgcccccgt tttacccctg
     5701 cacatttgtg aacgccagca gagtttttta tgcgattgca gatatggaaa gcggctcgga
     5761 gagaaaagca aaagatggaa aagttacaaa aaaaaatcgc gagatacatc gcataaaaca
     5821 acgccacccg aaggtggcgt gcttttatta ttacttatcc ctgtctaacc agtcgccgct
     5881 ttcaattagc tttaatccat cgacgggtcg ttgataaacg tacatccatg cactcccata
     5941 cggcgtctga atcaactggc gcgcgtattc accgcccctg gtgcgcaagg catcaagttc
     6001 ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt accgttccgt tccccggaac
     6061 tgcgcctgga tagtggccca ggctatacaa ctggtagtta tcgatactga aatcgcccag
     6121 taactgggca ttggtcatcc agtgactgtt gccttgtttg tggcgtaaac tgccgtagac
     6181 aaatattcgc attgctaaaa ctcgaactga tagagcaaat ccagtgcctg gtctacacca
     6241 gacacggctt ccagatatag cttaggcatc aggcgataac gtaacgtgag tgttgctata
     6301 gagtcaaata tacccacgcc gtatttcact tgcagacctg gcaatacata gccgctgacc
     6361 actacctggg aggagtcgcc tactcccagg gtgtcgagcg ctaaattgct tacgccaaac
     6421 gtctcgccga ttttacccac aatctggcca ctttgcgcaa cccccagacc aatcagcatc
     6481 gaggtcattg ccgcactgtc gctctgatcg ctctccagcc cttgtccacg tagcaaataa
     6541 gacaatgcag cttgttgcga catcgccggg tcagagaaga tctccgcttt cggttcgtcc
     6601 gccagaccag tgacgcgaac tccggcgatt acatcgtctt ctgtagcatc cgggttacga
     6661 atagcttcaa tattaagata cggttgatcc ggcggaccag agaacagtaa ctcaccctta
     6721 cgcacaatca gatcctgacc ataggcatgg aagcgccctt cagggatgtt gatctgcccg
     6781 ttcaggccca gcccttgttt gtcctgtacg acgttaagat cgcccgtcag ccgcgctttc
     6841 aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga caatcaggtt actgttaatc
     6901 ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat cgttaagcat caccacatcg
     6961 ctggagacgc ctactgcgct ttccggcaga tcgtgcacca cgatgcgcgc ccacgggaca
     7021 tccacgcgac catcgagggt aaacaggttt ggtgtagcct cgaatacaac atctggcgat
     7081 acatccattc gtaccatcgg cggcacggtg atccgcactt tactgccttt cgccgttact
     7141 cgtgcccgcc agttttcaat ttggctccag tcggcatcac cgttcaggta gatttcaccc
     7201 tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac cgttaaagtt gaccgcaagc
     7261 tggctcggct gcatatcaaa cggcataaag ttgccgtcga tatccacacc cgtaacctga
     7321 agctgaccaa acaactgcgg gctttgcaca tcaccaccca gacgcaagtt ggcactcacc
     7381 atccccgctg ctttttcccc acgggtaaag atggggttta tcatcgcaag gttgaagtta
     7441 cggatattga cgttgccacc aagattacgg cggccttgcg gatcggtcac ctgcacctgt
     7501 ccatcaaact ggccgttatt ggtcaggcgg atggtccagc ccaattcggc acggttgtta
     7561 cgcaattccg ccgtcagatt cagtgtctga aacgccaccg gcagcgccgc atcgttgacg
     7621 gtttgcgtta cctgcacgtt acgccccgaa agggtgatac tgccctgcgg cagcccctct
     7681 ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga taccgctggc ctgagtggtt
     7741 tctggcataa acggtttcag catggcgagg tcgaagcggt tgagattcac caccgcacgc
     7801 ccttcggccc ccgcatcgat agtttgcggc acgcacagtt ccgcattcgg gttaagccag
     7861 cagtgtggcc cgatgctgat tttttgctcc ttattgcggt aatccagcgc aatatcgcgg
     7921 gtcagcgacc acgggccgac cggcgtctgg aagcgggtat tgctaagagt tcccttccag
     7981 cgttcttctt tgcgatcaaa acttcctgcc agattaagct gcccggagac aggctcgccc
     8041 tgaatccgca actgtagctc gtgctgcttt tcgctgcctt tggcattcag ggtgacgagg
     8101 ttgatattta catccggctg cgaaatttgc tcaacgcgta cgtcgagttt cccggcgatc
     8161 tgatcggtgg atttaatgtc gccttccacg cgaacctgcg ccacggaaag ttcctgccag
     8221 cgcaggccgc gcgcggtgat atctgccagt agttgtggcg cttccaccgt gccgcgtact
     8281 ttcaccagcc ctttcgctgt accgccaagc cccggcagcg cgttatccag ccccggcgcg
     8341 ttgatggtgg catcaagatt gagatctttt acccccagct cgcctttcac ttcggcactg
     8401 tttggcccga gttccagatg aagcccaggg atcatccact gcatataact gttgcctttc
     8461 agcgtgccgt caacgttcac tttgttctgt ttgacgttac cggtcagctt cagttctggc
     8521 acctccatct gccaggtgcc gccgtacagg ctaccgcggg ttttaatcaa gccattgagt
     8581 ttcgacggcc actccgggaa ctctttggcg gtgttaatgc cattaagcgt tagctcaccg
     8641 cgccaactaa tggcctgctg ccagtcgagc aacgccttga gttcagtttt cccttccagc
     8701 gccgcgacgg tgagtttgtc gagattgacc tgctgttcat tacctttggc atcaagggta
     8761 atggtggctg gcgggatctc ctgtcccttc actgccgtac gcatagagag cgtgtaatcg
     8821 gtcattttgc cggtaagttt cagtttcaga tcatccgcct gatactgctt ctcaccagtg
     8881 aacggccagt aaagctgttt gctgttcact tccacgttga gtggcaatcc ggcttccgcc
     8941 agtcgcgtct gggcgcgtaa atccatatcc accggaccgg aaaggttaac gcctatctcc
     9001 agctgttcgc gcagcgcgcc gcccactttc agcttcacct tttcaccttt caacggctcc
     9061 acgttcagtg tgctgttgag ggtgatatcc accggccagt tgtctgacag ctgcgccgtg
     9121 ccgctggcgt tgacgatccc ctggttggaa tcgatatcca gggcgtccag tttagtattg
     9181 ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg tgatgtccgt gtcgcccgtc
     9241 acgcgcagct gctcgccctt aaactcttca atgttcaggt taagcggcaa atgcacgtcg
     9301 gtcatttccg gcaataccgg gcgagaaaag agatctttca gcgtttcgcc gagcggcttt
     9361 tcctccggct gcggattttc aattttcggt tcgaccactt cttcctgcgc cacttccacc
     9421 actttcggca gagcaatcag caggcctttc agcgacgtcg gtttcagggt cagggttttc
     9481 tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca ccgtggtgtc atcaatcttg
     9541 atgttgacgt tgtccagcgc tacccgtgtc agggtgatgg gatacggcgt ggagagatcc
     9601 agcggaccgc tatcttcctc ttcttcaacc tgttcagaag gaggcatttt tttactgtcg
     9661 atgttgacct gaatgtcttt cagcgccagg tcattaatac aaacgctact gttccacagg
     9721 cactcaagac cgaccgccag atgcagatta cccgctttta ccgccacgcc tggctgctca
     9781 taacgaacgt cagacaaggt gagatcgcgc cagccgccgg tgaccttgcc aatatccagt
     9841 cctggcaccc agcgatccgc cgctttaaat acaagatgca ggccgctggt ggtgcccacc
     9901 agaaacgcca ccgatcccag caacagtaag ataacgataa ccacgccgag gctgattttt
     9961 ttccataaac tcataattct ggccccagac cgatgtaaaa ctgtaacccg tgttcgtctt
    10021 tatccgcgac cggtacggca aaatcgagtt tgattggccc gaccggcgat tcccagcgta
    10081 cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat atcgcttacc gcttcgccac
    10141 tatcgacaaa caccgcgccc caccattttc cggtcacgtt gtactggtat tccagcgatc
    10201 cggttatcaa cttcgaggcc cctttcaggt caccgttggc gtatttcggg gcgatagatt
    10261 tgtatttgta gccgcgaata ctgcgatcgc ccccggcgaa gaaacgcaga tccggcggta
    10321 ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc gcgtgtcaca aagcgatggc
    10381 gatcgtacag tgtgcggatc cagacgttct gcgcctggaa aacggagaaa tcgacatctg
    10441 aaccccaggc cgtgttggag tagtcgatag agtagcgttg cgagtcgccc caggttggca
    10501 tcaggccacc acgagaacgc gtgcggctaa tcatcacccc aggataaaac agcatcgtgg
    10561 tgttggtaat ttcaccctgg gtaaagtggt cgaggctcca gcgcaggtta atggcacgct
    10621 gccagccgct ggagagatcc cagtagcgag aagccaccag cgtagtggag tcagattcgg
    10681 tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa ataatattgt tccagtggat
    10741 tcttcagcag cggcatttta tagctgaagt cgagggtctg ttccggcgcg gaaatactgg
    10801 tactggtggt caaactgtga ccgtatgagt tcatccacgg ctttttccac gtcgctttca
    10861 cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc gatggtgttt tctgttcgcg
    10921 gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg cgctttatca aattgtggag
    10981 ccaccaccac cgagttaaac cagccggtag cagaaagtcg gcggttcagt tccgccagat
    11041 ctttcgattc atactcatcg ccctctttaa acggcaccag attttgcagg tattcatcgc
    11101 ggatttgtga tccttcaaag gtcacatgcc caaagcggta acgttcgcca ctgttataat
    11161 caatatccca gaaggcttta tgcaggccga gcgcaatgcc cagctgcgct ttggtaaatt
    11221 cgctatcgaa ataaccttta cgcaacgcaa tactggttaa ggactttttg aaattttcat
    11281 aatcgccctg gttcagtacc gtaccaatag ccgggcgagt atcgagcaat ttcaaatagt
    11341 ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt gccgccaatt aacaccggca
    11401 cacctggcgt gactttggcg atcaatacct gccgcccttt ctttggcggt ggacggagat
    11461 caaattcaat ggtcggctgg taataaccca gcgctttcag accttcacgg atggcatcat
    11521 cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc actttcaatc gtagaaagct
    11581 gcgcacgaac gttcttttcc agctgtcccg ataacccctc gacctgtaga cggacgttcg
    11641 cggcgacggc agatccgctt aagcagagta agcttacaca gcataactgt cggatatagc
    11701 gcacattttc tcctgaatat ccttttttcc tgcccctgga acgccgttaa acggcttaac
    11761 aaaaatccag taatatggat taaaaagcag actaaacccc aaatatttct tatgttttac
    11821 tttagaccta ttcacggtgg ttattgtgtg caaatacgcc tcttgttaca accttaaccc
    11881 caatgaccga ttttcgggag agcgacacca tgagtttatt tgataaaaag catctggttt
    11941 cccccgccga tgccctgcct ggacgtaaca ccccgatgcc cgtagccacg ctgcatgcgg
    12001 tcaacggtca ctcaatgacc aatgtacctg acggaatgga gattgccatt tttgcgatgg
    12061 gttgtttctg gggtgtggag cgtctgttct ggcagttacc cggcgtttac agcaccgccg
    12121 caggctatac cggcggctat acgccaaatc cgacttatcg ggaagtgtgc tccggtgata
    12181 cgggtcatgc cgaagcggta cgcattgttt acgatccttc cgtcatcagc tatgagcagt
    12241 tgctacaggt attttgggag aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc
    12301 acggcacgca gtatcgttca gcgatttatc cgctgacccc agaacaggat gccgcagctc
    12361 gcgccagtct ggaacgtttt caggcggcga tgcttgccgc cgatgacgat cgtcacatca
    12421 ccacggaaat cgctaacgcc acaccgtttt attatgccga agatgaccac cagcaatatc
    12481 tgcataaaaa cccatatggt tactgtggaa ttggcgtctg tctgccgccg gaagcatagc
    12541 gttacgggta caaatgtagt ttgttgataa agtgctcttt gtctatgcct gatgcggctt
    12601 aaacgactta ccaggcctac aaaatcgtcc aaattcaata tattgcaagg actgcgtagg
    12661 cctgatcggc atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct
    12721 ctggcgactt tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt
    12781 cgaatcggct ttcaatgtgt atttcacaca aattaatcaa ctttcccttc cgaggatctg
    12841 gcctgaaagg tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct
    12901 gtaagtgcgt tcttctcgat gtccgagatc tcgcttgccg cctcacgcaa aatcaaactt
    12961 aaactgctgg ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat
    13021 cccggcatgt tctttaccgt ggtccaaatc ggtctgaacg cagtggcgat tctcggcggt
    13081 atcgtcggtg atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg
    13141 gcagagctct ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt
    13201 atcctgtttg cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct
    13261 ttgcgtatca tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc
    13321 ttcaacggcc tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat
    13381 gacatcactt ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta
    13441 cgtaaacagg aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg
    13501 tcttcaatga caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc
    13561 ctgaagaata aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt
    13621 gaccacatca tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa
    13681 agcctggcac tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta
    13741 accctttcag aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg
    13801 aacgagtacg cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg
    13861 ggcgatctgg tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg
    13921 ctgattgacg gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc
    13981 ccgcagtcgg gcaactacga aaccatcggc ggctttatga tgtttatgct gcgtaagatc
    14041 ccgaaacgca ccgattcggt gaaattcgcc ggctataaat ttgaagtggt ggatatcgat
    14101 aactaccgca tcgaccagct gctggtgacc cggatcgaca gcaaggccac cgccctttcg
    14161 ccaaaactgc ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca
    14221 acggctcctg aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg
    14281 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    14341 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    14401 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    14461 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    14521 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    14581 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    14641 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    14701 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    14761 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    14821 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa tcggtcagcg
    14881 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    14941 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    15001 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    15061 gttaccgcac gatcgttacc agaccaccac cattgttaac accgacgacg cgattccggg
    15121 ttccggcatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    15181 gtttattgtc gatagcaatg gtgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    15241 cgctgtagtg gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgctcac
    15301 accggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaata
    15361 acataaattc tggaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    15421 ctctgagttc ttacaccgaa ctaatactgg taacgctagt tgctcttttt taaacgcctc
    15481 aatggtcgat ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    15541 attcgcactt actatcgcat ccacttgttc ggctaattcg acttgttggc aacctgcatt
    15601 aaccagaaaa ttataaataa gagagtaatc ttcatcctcc aaaacaacag aggaaccatt
    15661 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    15721 ggcaaaatta tcatcaatta ttttttcgta aatataccat ccatcgcatt ctttaattcc
    15781 accgcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta catcctccaa
    15841 taaggcataa ccgaaagata cgtcaccaaa cgcctggcga atataacctt taatcctttt
    15901 tggtatgtca gactgttttt tctgcaaaat atcacaggca tacaatcttc tgttttgttc
    15961 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    16021 aatttcttcg tattcaaacc cactgcctcg caatgtcatt gtatattcag caccgttatc
    16081 tttaaggctg aaactgataa gaaaattatc catggcatta acagtttcat tttttgatga
    16141 aagcacttga taactatttt tatcaatctg tgttgcgcaa cgaaaggact cccgcaaagt
    16201 taacagacta tcataattcg aaacccaatc ttttttctta aatgcatgct gaatattatt
    16261 aaaaaaattg tttgaatgtg tttttgtatg cgatgacttt acaaataact ccaccgcacc
    16321 aataaaatta ttgtgtctga tcctgggtaa catatggcta ccttatttta tttggttatt
    16381 ttatgtcgcc attaaatatc tcattataga agtaaactat caaaaaacgc tcattattaa
    16441 aatgtaactt attagcaatt gaatttttgc actttaaata caatctcttt ttatttataa
    16501 ctttttaggt tgggataaat caggatggca gaatcaggaa atacactgtt tctgccatct
    16561 gaatttagta aatggacact ctgaaccccg gattcaggaa cgactcgcgc ggagtgtaat
    16621 ccagcggttt accctgccag tcgtgaacgt gcgctccggc agctgcagcc acagcatgtc
    16681 cagcggcggt gtcccaaata ttcgttggtc cgaagcgcgg gtacagctgc gcctgtcctt
    16741 ccgccaccag gcagaatttc agcgaagagc cgatggacgt ggtctgatgt tcgccaagct
    16801 gttgcagata ctctttcagc tccgcatctg catgggaacg gctgataacc accagcggcg
    16861 ggcgcgcatc gcggacctga atcagcttgc gcacaccgca ctcttctttc caggccttgc
    16921 cttctgccgc gctgtacatt acgttcatta ccggcgcata caccacacct aaaatcggtt
    16981 tgccatggtc aatgagcgca atgttaacgg tgaattcgcc attacgttta ataaactctt
    17041 tagtaccatc cagcgggtct accagccagt aacgctgcca gtgctgacgg acttcccaac
    17101 cgggaggatc ttcttcagaa aggaccggaa tatccggtgc cagcgtacgt aaaccgtcca
    17161 tgataacggt gtgagcggca atatccgctg ccgttaccgg agaattgtcc gctttgctga
    17221 cgacgtccat cggtttcgtc ccgtcgtaga cctgcataat ggcatcgcct gcattccgtg
    17281 caagctggca tacttgatct aacatttctc cacctcgtct ctgtgagcgg tgttaactta
    17341 ttgttttact tataccctat cgttaatgaa tgcgccaact gtgatagtgt catcattttc
    17401 aaagcgtaaa attgtggcat tcttcactgt tctataagta aggcgtttat tctccctttt
    17461 ctttcgtatt cccgatgata aaaggatgtc cctgatgatt aagtttagcg caacgctcct
    17521 ggccacgctg attgccgcca gtgtgaatgc agcgacggtc gatctgcgta tcatggaaac
    17581 cactgatctg catagcaaca tgatggattt cgattattac aaagacaccg ccacggaaaa
    17641 attcggactg gtacgtacgg caagcctgat taacgatgcc cgcaatgaag tgaaaaacag
    17701 cgtactggtc gataacggcg atttgattca ggggagtccg ctggccgatt acatgtcggc
    17761 gaaaggatta aaagcaggtg atgttcatcc ggtttataag gcgctgaata cgctggatta
    17821 tacggtcggt acactcggca atcatgaatt taactacggt ctggattacc tgaaaaatgc
    17881 gctggcggga gcgaaattcc cttatgtaaa tgccaacgtc attgacgcca gaaccaaaca
    17941 gccaatgttt acaccgtatt taattaaaga taccgaagtg gtcgataaag acggaaaaaa
    18001 acagacgctg aagattggct atattggcgt cgtgccgccg caaatcatgg gctgggataa
    18061 agctaattta tccggaaaag tgacggtgaa tgatattacc gaaaccgtgc gcaaatacgt
    18121 gcctgaaatg cgcgagaaag gtgccgatgt cgttgtcgtt ctggcgcatt ccgggctgtc
    18181 tgccgaataa agtaatggcg gaaaactcag tttattacct cagtgaaatt ccgggcgtta
    18241 acgccattat gtttggccat gctcacgccg ttttcccggg taaagatttt gctgatatcg
    18301 aaggggctga tatcaccaaa ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt
    18361 ggggcgatca tcttggggtg gtcgacttac aactcagtaa tgacagcggt aaatggcagg
    18421 tgacgcaggc gaaagcggaa gcacggccga tttacgacat cgccaataaa aaatccctcg
    18481 cggcggaaga cagcaagctg gtagaaacac tcaaagccga tcacgatgcc acacgccagt
    18541 tcgtcagcaa gccaatcggt aaatctgccg acaatatgta tagctatctg gcgctggtgc
    18601 aggacgatcc gaccgtgcaa gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca
    18661 ttcagggcga tccggatctg gcaaaactgc cggtgctttc agctgccgca ccgtttaaag
    18721 ttggtggtcg caaaaatgac ccggcaagct atgtggaggt ggaaaaaggc cagttgacct
    18781 tccgtaatgc cgccgatctt tatctctacc ccaatacgct gattgtagtg aaagccagcg
    18841 gtaaagaggt gaaagagtgg ctggagtgct ccgccggaca gtttaaccag attgctccta
    18901 acagcacgaa accacagtca ctcatcaact gggatggttt ccgcacttat aactttgatg
    18961 ttattgatgg tgtgaattat cagattgatg ttacccaacc cgcccgctat gacggcgagt
    19021 gccagatgat taatgccaat gcagagcgga ttaagaacct gacctttaat ggcaagccga
    19081 ttgatccgaa cgctatgttc ctggttgcca ccaataacta tcgcgcttac ggcggcaaat
    19141 ttgccggtac gggcgacagc catatcgctt ttgcttcacc ggatgagaac cgctcggtgc
    19201 tggcagcgtg gattgctgat gagtcgaaac gtgcggggga aattcatccg gcggcagata
    19261 acaactggcg tttagcaccg atagctgccg ataagaaact ggatatccgt ttcgaaactt
    19321 ccccgtcaga taaagccgca gcgtttatta aagagaaagg gcagtatccg atgaataaag
    19381 tcgcgaccga tgatatcggg tttgcgattt atcaggtgga tttgagtaag taaaacactt
    19441 ctttttcggc ctataaatca accgcatccg gcatttattg gcggatgcga tgctgccgta
    19501 tcttatccac cctacaagcc atgaaccgta gaccggataa gcgcagcgca tccggcagtt
    19561 atgccgcacg ttcatcccgc accgccaaca cctcgggcaa attcaactca atccagtccg
    19621 ccagtgcagc aaccttttcg ctcacctgct cgcccagcgg cgtgaggcta tattccacat
    19681 gcggcggcac caccgaatac gcgatacggt taagaaaacc atcctgttct aacgcctgta
    19741 acgactgcgc aagcatcttt tcactcaccc caccgatttt gcgccgcagg tcgctaaagc
    19801 gatgagtacc ttcgcgtagc gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt
    19861 tcaacacctc gcgcgacggg cactgttccg caaagagatt accctctttc agttgttgtg
    19921 acaggctaac ctgactcatt tcatacttac ctttttgtat gtacttacta aaagtaagtt
    19981 taggtgttag cgtatttaaa cacaagacaa aacgatggag acttcccatg atcgctatta
    20041 ctggtgccac tggccaactt ggtcactata ttattgaatc cttgatgaaa acggttcctg
    20101 ccagccaaat agtggctatc gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag
    20161 gcattaccgt gcgtcaggct gactacagcg atgaagccgc actgacatct gcacttcagg
    20221 gagtggaaaa actactgctg atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc
    20281 gtaatgttat taatgccgca aaggcggctg gcgtgaaatt tatcgcttat accagcctgc
    20341 tacatgcaga tacctccccg ctcggcctcg ccgatgagca catcgagacg gagaaaatgc
    20401 tggctgattc tggcatcgtt tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc
    20461 tcgccagcgc cccggcagca ctggaacacg gcgtatttat cggtgcggcg ggcgatggca
    20521 aaatcgcctc agcaacgcgg gcagattatg cgacagctgc ggcacgcgtg attagcgaag
    20581 ccggtcacga aggcaaggtt tacgaactgg cgggcgatag tgcctggacg ttgacacagt
    20641 tagcggcaga gctgaccaaa cagagcggca aacaggttac ctatcaaaat ctgagcgaag
    20701 ccgatttcgc cgcggcactg aaaagcgtcg gactgcccga cggactggcg gatatgctgg
    20761 cggattctga cgttggcgca tcgaaaggtg gtctgtttga taacagcaaa acgcttagca
    20821 aattgattgg ccgcccaacg acaacgttag ccgaaagcgt aagccatctt tttaatgtta
    20881 ataactagtt aattaaagtg gcatcctccc gcatcctctc tgataatgac gggatgccgg
    20941 gagcaatcat gtctgcttcc tgaacgtttc ttctgacaga ccaatggatg ccagtaatga
    21001 ttagcggcgt gctgtacgcc ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc
    21061 cgttgatcgt gccggaatac ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag
    21121 ggttaattgc gctacccatt gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc
    21181 gggactggct taccgccttg atgctcacta tgatgggcaa cctcatttat tacttctgcc
    21241 ttgccagtgc cattcaacgt actggcgcgc ctgtttccac gatgattatc ggcaccctgc
    21301 cggtggtgat tcctgtcttt gccaatctgc tttatagcca gcgcgacggc aaactcgcgt
    21361 ggggaaaact cgccccggca ctgatttgta ttggcatcgg cctggcgagt gtgaatattg
    21421 ctgagttaaa ccacggactc cccgattttg actgtgcacg ttatacctct ggcatcgtgc
    21481 tagcgttagt ttccgtggtc tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc
    21541 gggaaaatcc cgacaaacat ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc
    21601 cagtttctct catcggctat ctcgtcgcct gttactggct gaatatacaa acgccggact
    21661 tctccttacc ttttggcccc cgtccg
//
LOCUS       DADLSE010000057        21640 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLSE010000057 DADLSE010000000
VERSION     DADLSE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.057"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN692_002165"
     CDS             complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN692_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7264475.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRLAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSG"
     gene            complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN692_002166"
                     /pseudo
     CDS             complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN692_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN692_002167"
     CDS             complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN692_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7264476.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            2985..3086
                     /locus_tag="JN692_002168"
     CDS             2985..3086
                     /locus_tag="JN692_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264477.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN692_002169"
     CDS             complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN692_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7264478.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(3430..4167)
                     /locus_tag="JN692_002170"
     CDS             complement(3430..4167)
                     /locus_tag="JN692_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7264479.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4291..5475)
                     /locus_tag="JN692_002171"
     CDS             complement(4291..5475)
                     /locus_tag="JN692_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264480.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCMTFANFSILWTSMAFLLAAPPFNYSDGDIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGATGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(5667..6659)
                     /gene="proX"
                     /locus_tag="JN692_002172"
     CDS             complement(5667..6659)
                     /gene="proX"
                     /locus_tag="JN692_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7264481.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KSGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(6717..7781)
                     /gene="proW"
                     /locus_tag="JN692_002173"
     CDS             complement(6717..7781)
                     /gene="proW"
                     /locus_tag="JN692_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7264482.1"
                     /translation="MADQNNPWDTTPAAGSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(7774..8976)
                     /locus_tag="JN692_002174"
     CDS             complement(7774..8976)
                     /locus_tag="JN692_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7264483.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(9331..10290)
                     /gene="nrdF"
                     /locus_tag="JN692_002175"
     CDS             complement(9331..10290)
                     /gene="nrdF"
                     /locus_tag="JN692_002175"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7264484.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(10300..12444)
                     /gene="nrdE"
                     /locus_tag="JN692_002176"
     CDS             complement(10300..12444)
                     /gene="nrdE"
                     /locus_tag="JN692_002176"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7264485.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(12417..12827)
                     /gene="nrdI"
                     /locus_tag="JN692_002177"
     CDS             complement(12417..12827)
                     /gene="nrdI"
                     /locus_tag="JN692_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7264486.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(12824..13069)
                     /gene="nrdH"
                     /locus_tag="JN692_002178"
     CDS             complement(12824..13069)
                     /gene="nrdH"
                     /locus_tag="JN692_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7264487.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPQAASA"
     gene            complement(13317..13646)
                     /locus_tag="JN692_002179"
     CDS             complement(13317..13646)
                     /locus_tag="JN692_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7264488.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            13798..14142
                     /locus_tag="JN692_002180"
     CDS             13798..14142
                     /locus_tag="JN692_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7264489.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(14179..14628)
                     /gene="alaE"
                     /locus_tag="JN692_002181"
     CDS             complement(14179..14628)
                     /gene="alaE"
                     /locus_tag="JN692_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7264490.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            15296..15700
                     /gene="stpA"
                     /locus_tag="JN692_002182"
     CDS             15296..15700
                     /gene="stpA"
                     /locus_tag="JN692_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7264491.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKFLDDFLI"
     gene            complement(15747..16271)
                     /gene="ygaP"
                     /locus_tag="JN692_002183"
     CDS             complement(15747..16271)
                     /gene="ygaP"
                     /locus_tag="JN692_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7264492.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(16281..16580)
                     /locus_tag="JN692_002184"
     CDS             complement(16281..16580)
                     /locus_tag="JN692_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7264493.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            16763..16921
                     /locus_tag="JN692_002185"
     CDS             16763..16921
                     /locus_tag="JN692_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7264494.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            17005..17454
                     /gene="kbp"
                     /locus_tag="JN692_002186"
     CDS             17005..17454
                     /gene="kbp"
                     /locus_tag="JN692_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7264495.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVLEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(17455..18117)
                     /gene="csiR"
                     /locus_tag="JN692_002187"
     CDS             complement(17455..18117)
                     /gene="csiR"
                     /locus_tag="JN692_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HAY7264496.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(18138..19478)
                     /gene="gabP"
                     /locus_tag="JN692_002188"
     CDS             complement(18138..19478)
                     /gene="gabP"
                     /locus_tag="JN692_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HAY7264497.1"
                     /translation="MLSIAGVIGASLFVGSSVAIAEAGPAVLLAYLFAGLLVVMIMRM
                     LAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFWVLVIPLEANIAAMILHSWVP
                     GIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKVIAILAFIFLGAVAISGIYPY
                     AEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAEIVTIAAAESDTPEKHIVRAT
                     NSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSVLELLNIPHAKLIMDCVILLS
                     VTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYY
                     APAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIG
                     FITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            complement(19811..21091)
                     /gene="gabT"
                     /locus_tag="JN692_002189"
     CDS             complement(19811..21091)
                     /gene="gabT"
                     /locus_tag="JN692_002189"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HAY7264498.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTRHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIMIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFDEAKQ"
     gene            complement(21105..>21640)
                     /locus_tag="JN692_002190"
     CDS             complement(21105..>21640)
                     /locus_tag="JN692_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7264499.1"
                     /translation="AEKLQQAVSKLHIGDGLEKGVTIGPLIDEKAVAKVEEYIADALE
                     KGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLAPLFRFKDEADVIAQA
                     NDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVAPFGGIKASGLGREGS
                     KYGIEDYLEIKYMCIGL"
BASE COUNT         5441 a         5615 c         5487 g         5097 t
ORIGIN      
        1 aaccagacag cgtaatggtg gtcagcggca taaagaagca ggccaccgca aacccctgga
       61 taaactgcgg ccaggccgac gcgccaaaat ccatacctgg ttcaaaggta taggcacgcc
      121 agtagaagca gacggcatac ataataaagc tgaaggttac cagccgccgc atatccagtt
      181 tatgcgcgag gcggccgata atcggcgaca gaatcaccgg aataatccct accggcgcag
      241 aggccaaacc tgcccaggtc gccgtgtaac cgtagacctc ctgcaacaac tgcggcagca
      301 gaacaatagc gccgaagtag agcatatacg cgaggctgat acacaagcag ccgatggtga
      361 agttgcgcga cttaaacaac gacagatcga ctatcgggtt atcgtcggtc agctcccaga
      421 caatcaggaa gcagatagcc accaccgcca ccacggtaag aatgataatt tcctgtgatg
      481 aaaaacagtc cagctcttta ccgcggtcga gcataatctg caggctgccg ataccaataa
      541 ccagcagtgc cagccccacg gcatcaatcc gccgccgttc ggtgcgggtt tcacgtccgc
      601 gcagagtttg cagtgtcatc aacaccaccg ccacgccaat cggcacgttg atgaagaata
      661 tccagcccca gtggtaatta tcgctgatat aaccgccgag gatcgggccg caaattggcg
      721 cgacaatcac cgtcatcgac cacaacgcca gcgcgatcga gcgtttggct ggcgggtagt
      781 tattcagcaa tagactttgc gaaagcggga tcaacggccc ggcgacaatc ccctgaatca
      841 cgcggaagaa gatcagcata ttcaggctgc tggagacacc acacgcccac gacgcaatag
      901 caaaggcgat ggtggaccaa aggaacagtt tcacttcccc gacgcgcttt gccagccagc
      961 cggtaagcgg gatcgagatg gcattcgcca ccccgaaaga agtgattacc cacgttccct
     1021 ggctgagcga tgagcccaga ttcccggcga tagtggggat cgccacgtta gcaatggtgg
     1081 agtccagcac ctgcatgaat gtcgccagtg acagcgcaat cgtcataatg accagttgcg
     1141 cgccttccag cggtttttgc tgttgcatca cacgcacctc tggattagcc agcgttagct
     1201 tttacgatat cgtcgatcag tttattgaca ggtgccaggc tgatttcacg cgcggtactt
     1261 accgcgaccg gtgttgaacg tactttattt gccagtacct gaccgtcacg gttagtggta
     1321 ttgacgctca ccagcgtgga caaaccaatg cgcagcgggt attgctccag ctgtttctgg
     1381 tccagttcga tacgcacagg tagacgctga acgactttga tccagttacc ggtcgcattt
     1441 tgcgccggaa gcagtgagaa cgcgctacct gtgcccatat ccagaccaac cactttaccg
     1501 gtgtatttca catcatcgcc gtaaatatcg gtggtgatgg tgaccggctg accgatgcgc
     1561 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
     1621 accgccatca gcggcgtcgt tgggctaatt tgcgcacccg gttgcacggc acgacgggaa
     1681 acataaccgg tcatcggact gacaatacgg gtacgctcca gcgccagcta ggcgttacgt
     1741 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
     1801 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
     1861 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
     1921 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tttgcacctc aatattcgcc
     1981 tgcaactgtt tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
     2041 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
     2101 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
     2161 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
     2221 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
     2281 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
     2341 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
     2401 gcttgccaaa aaggccagca tgttgcgatg ctggccggtc attttttctt ttataaatct
     2461 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     2521 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     2581 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     2641 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     2701 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     2761 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     2821 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     2881 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     2941 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     3001 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtga
     3061 caaataaagt aaatgtattg ttttagaaaa atgattcttg tgggttatat aatcgccatc
     3121 actttccagg cgagcccata ggccagcgca ctaagcagtg ttgggatgat aatgctgcgt
     3181 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     3241 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     3301 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     3361 cgcaggcgca gcggcaaata gcggaagcaa taattcgccg cgccaaccag taacccaagc
     3421 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     3481 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     3541 aagggcacca actaaagctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     3601 caggaaactc ataaagagtg ccggaagcat aaaacctaat gcagcttcaa cggcgggata
     3661 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     3721 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     3781 cagttttgcg gtggcggcgg caaaaacctc atccgtcagg ccaaacgccc atagggcggt
     3841 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaaaacatg
     3901 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     3961 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     4021 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     4081 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccagg
     4141 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     4201 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     4261 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     4321 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     4381 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggtggcac cgccaataaa
     4441 gtagctggtc atgtaacctg cggtcaggcg attacgcgca tcaggatgta ttcgataaat
     4501 taccgtctgg ttagtgatat gcacgccctg tacggtgagg tccagcacca gaatgccgat
     4561 aatcaacgcc agtacggaag tgtgaccaaa ccagattgcc agccatgaaa gtaacagcag
     4621 aagcagaccg aaagttgtgg tgtggtgcga tttgcccttg tcggcaaaac cgcccgccgg
     4681 acgagcgccc aacgctccgg cagctcccgc aagcccaaac agaccaatgt caccatcgct
     4741 gtagttaaaa ggtggagcgg caagcaaaaa ggccattgag gtccagagaa tgctgaagtt
     4801 ggcaaaggtc atgcagccca gcaacgcgcg ggtgcgcagg atcttatcgc tgatgaacat
     4861 gctgaaaacg gaacccaaca actgtgggta gttgaggtgg gtttctgatt tcatttgtgg
     4921 cagaccacgc cataatgcca gcgccatcag tgccattaac accgaagcaa cccaaaagac
     4981 ggtgcgccag ccgccgaggt ttgccagtaa cccggcaacg gttcgtgcca gcaatatccc
     5041 caacagcaga ccgctcataa tggtgccaac cactttgccg cgtttatccg gtgaagccag
     5101 cgtcgctgcc agcggaacca gaatttgtgc cacgactgag aataaaccgg ttaatgccgt
     5161 accgaggatc atcatcgcca gtgactgact gctggcagtg attaacatac cgcccgctgc
     5221 cagtaaggtc atcgagacaa tcaggcggcg gcgttcaaac atatcaccga ggggaacaag
     5281 aaacagtaga cctgcggcat agcccaactg cgcggcggta acaataaagc ctgccgaact
     5341 ggcggaaagg gaaaagttac gcgcgatggt gtcgagcaat ggctgggcgt aatagttact
     5401 ggctaccgcc agaccggtgg cgatagacat cagcacgatc agcgccgggc taagctcatg
     5461 attaggttta gtcattattt ttgttcatca ggtgatgatt aagctggaaa tcaataatag
     5521 cggatgttga tgaatatggg atgaaagata gcttgttgaa atgtgcgatg tggtttgtag
     5581 gcatgatagg acgcgtcagc gtcgcatcag gcattgtgta cagagcggcg gatgaggcga
     5641 aaacgcctta tccgcccgaa taaaaattac ttctgcgctg ccagcgcctc attcacccag
     5701 ccatcgaact gctgctggtg ggctttgatc cagccatcaa cgtggccctg aatatcgcct
     5761 tctgaggctt tgccgtcatg cataatggcg ttctgggcgt taatatcggc cactggcaac
     5821 tgcataatgg caaacagttt ggctgccgcc ggatttttct cggtccaggc tttgttggca
     5881 acgatatgca tggtgctgac cgggaagccg taattcgcgc cattcggcag tttggtatcg
     5941 gcatttttat cgcccggcag tgcggagaac ggcacctgca accagaccac atctttccct
     6001 gacttcagct cattactcac ccagtacggc gtccaggtgt aataaaacac cggtttgccc
     6061 tctttgtagc gactgatggt gtcggccatc atcgctgcgt agttcccctg attatgcgtc
     6121 acggtgttgg tcagttcata cgcggcaagc tggtggttga tcgcaccttc gcagccccag
     6181 ccagggttac aaccggttaa atccgcttta ccgtcgccgt tggtatcgaa cagtttggcg
     6241 atcttcggat ctttcagttg tgcgatgttg gtgattttgt attggtcggc ggttttctta
     6301 tcgatcaggt aaccctgtgc tgcgccgtta acaaataccc cttcacgata aaatttctta
     6361 tcgccaccgg cagcttcgta catgttgtca tggagtggcg tccagttcac ggcggtgaag
     6421 gttgcatcgc cggaagcaag cgaggtgtag ccaacgttgt aatccacttc gctgggtttg
     6481 ttgacggtat aacctaattt ctccagcgca cgactgacca gcagcgtctg gaaggtttct
     6541 tcagtgatgg tgctctgaac tggattaaca gtaatgcctt tgcccggcag atcggcagca
     6601 aaagtttgtg tagagataag cgtggcaaac gctgtcgcaa aaagtacgct atgtcgcatt
     6661 gttattcctt tttttggcag ggtgataatg cccggcgtca ccgggcaagt gcagagttac
     6721 ttaatgaatg ggcgggtcag cagaccaaca gggccagtgg tgtaccagcg acggttgccg
     6781 cgactgcgtg agtcgcgccc aacggcctgc gtcaggcgat cgaggataat ggcgaggatc
     6841 acaatcccga cgccgccaac ggtggcaagc cccatatcca gacgaccgat accgcgaagt
     6901 accatctgac ccagcccgcc gacggcaatc atcgaggcaa tgaccaccat agaaagggcc
     6961 agcatcagcg tctggttaac gcccgccata atggtcggca tcgccagcgg taactgaact
     7021 ttgaacagca tctggcgcgg gctggcaccg aatgagcgcg aggcttcaat cagatccgcc
     7081 ggaacctggt taatccccag aatggtcaga cggataatcg gcggcagcgc aaagatgatc
     7141 gtcaccacca cgcccggcac gttaccgata ccaaatagca tgacgattgg caccagataa
     7201 acaaacgctg gcgtggtctg catggcatca agcagtggac gaataatttt cgccgctcgc
     7261 ggacttctcg ccagccatat ccccaacggc aaaccgatga cgatacagaa cagcagggca
     7321 gttaacacca gcgccagagt caccattgcc tgcgaccagg caccgattgc gccgatggca
     7381 atcagcgaga ccagcgtcgc cacgcccatt ccgaccccgg aaatctgcca ggcgatgaga
     7441 gcgaaaacga taatcgccac tggcgctggc atacccagca gcaattgctg gaaaccgttg
     7501 aggatataat caaccggaac gcgcacgccc tggaagacgg gacggaaatg ggtaacaacc
     7561 cagtcgatcc cttcagtgac ccaactgtcg agcgggatca gcgttttatg gaacggatcg
     7621 agaatattaa aatgctcgac gtttggcgca ggcgtactgg tcagccagtc agcaccaccg
     7681 ccgtcagccg gtgcagtcgt cggtgtaccc caggcgtctg cggattgcgc ggcactgccc
     7741 gccgctggcg tggtatccca cggattattt tgatcagcca ttatttaccc cctcacgatc
     7801 taaagcgcgc agcagcattc ctttcgaaat gatgccgaca tactgttggt cctcgtcgac
     7861 cacgggcacc gcacagggtg cctgtccgac atgagagagc aactcgctaa gaggcgtttg
     7921 tgcatcgact gctaacggcg catcaatcag cgccgcatca agaccttgct gctgcgttaa
     7981 cgcggtttta agcgaatcga tggagactgc gccgacaaac ttattaccgc gttcgataac
     8041 gtagccatat tcgcgatctt catcctgcaa taatttcagt gccgaacgtg ggccgaagcc
     8101 aggagtttta cgaattaagc cattcggtgt ccggcgggca atatctttcg cactgaacac
     8161 ctgactaata tcaacgccac ggaagaaggt acggacataa tcattcgccg gattattgag
     8221 aatttcatcc ggtgtgccga cctgtaccac ttcaccattt tgcataatgg caattcggtc
     8281 gccaatacgc atggcttcat caagatcgtg ggaaataaag acaatggtgc gctgatgttt
     8341 cgcctgtaat tttaccagct catcctgcat ctcggtgcga attaatggat cgagcgccga
     8401 gaaggcttcg tccattaata atatatccgg attaatcgct aacgcgcggg ctaatcccac
     8461 acgttgacgc atcccgccag agagttcatc cgggtagctg tgggcataat tttccagccc
     8521 gacctgacgc agtgcatcaa gggctttttc ccggcgttct tcggcattaa ttccggccaa
     8581 ttccataccg aacgcagtat tgtccagcac ggtcatatgc ggcattaagg caaaggactg
     8641 gaagaccatc gcaatctttt ttctgcgcac ctcacggagt tcggcgtcgg atattttggc
     8701 aatatccaca ccatcaatca gcacttgccc gcgggtgggt tcaatcaggc gattgagaag
     8761 gcgtaccatt gtggatttac ccgagccgga taatcccatg atgacaaata tctcgccttc
     8821 ttcaatggcc agactggcgt cttttacgcc aagtgatagc ccagtttttt ccagaatttg
     8881 ttcttttgaa agtccttgtt cgatatattt gaacgctcgc tgtggatgct caccaaatat
     8941 tttataaaga tttttaattt ctaatttaat ggccatgcaa tagaatgatt cctttatttg
     9001 tttatgtcga tatattaccc aatggaaata gtcacttttt tctaccctaa catactgaga
     9061 atctgaggca acccctgagg gcaaatgtgg catgaatatt gatgcgagca aatcgccgga
     9121 attttccgtg atataagggc tgagagcaaa tcgagaaaag ttgatatttt ttgtcataga
     9181 tagcgaaaaa acgcctgaat attgtagttt caggcgaata atgcaacaga gtatcagtgt
     9241 agatcactac agaatatttg cgtgataaaa agctatttgg cgggaattat ttccctgctg
     9301 cgggtagtga tatttttgaa aataacaccc tcagaaattc cagtcttcat cttctgtttc
     9361 aaccgctttc cccatcacat aagaggagcc tgaaccggaa aagaaatcgt gattttcatc
     9421 ggcattcggc gaaagcgcgg cgaggattgc cggattcact tccgccattt ctgcgggaaa
     9481 taacggttcg tagcccagat tcatcaaagc cttattggtg ttgtaacaga gaaacgcttt
     9541 cacatcgtca gcccacgggg tttcggcgta cagctcatcg gtgtattgca actcgttgtc
     9601 gtagagttcc agcaacaaat cgaaggcgaa actcttcaac tcttcacgtt gtcccagaga
     9661 tatcttttcc atgtttttct gatatttata gcctatgtag taaccgtgga ctgcttcatc
     9721 gcggataatc agacggatca ggtccgcggt attggtcagc tttccgcggc tggaaaaata
     9781 catcggcagc cagaaaccgg aatagaacaa aaaagattca agaaacacac tggcgatttt
     9841 ctttttcagc ggatcatcac cgcgataatg ttgctgaata atctgagctt ttcgctgcaa
     9901 cggtgcgttt tcttcactcc aggcgtaggc ggcatcgaca tctttggtct ggcacagcgt
     9961 cgagaaaatc gaactgtaag agcgggcatg aaccgcttcc ataaagctga tattcgataa
    10021 taccgcctct tcatgaggcg tgagcgcatc gggcatcagc gaaggcgcgc cgataacatt
    10081 ttgcagcgtg tcgagcagcg tcaggccagt aaaaacgcgc atcgtcagtt gttgttctac
    10141 gacagttaat gtctgccagg cgggaatatc gttcgacagc ggcacctttt ctggtagcca
    10201 gaaattgctg gtcaggcgat tccacacctc cagatcttta tcgtcagata tcttgttcca
    10261 gttgatggcg ctgatacgtg agagtttcat agatattcct taaagtgcac aggagacgca
    10321 gccttcaatt tcagtgcctt ccagcgccat ctgacgcagg cggatgtaat agagcgtttt
    10381 gatacctttg cgccaggcgt aaatctgcgc tttgttgata tcgcgagtgg tggcggtatc
    10441 ggggaaaaac agcgtcagcg acagcccctg atcgacatgg cgagtcgctt ccgcgtaggt
    10501 gtcgatgatc ttttctgcgc caatttcgta agcgtcctga tacagcgcca gattttcgtt
    10561 agtcataaac ggggcagggt agtaaacgcg tcctgttttg ccctctttgc gtatctctac
    10621 tttcgccaca atcggatgaa tactcgacgt agcatggttg atataagaga tagacccggt
    10681 tggcggcacc gcctggagat tctggttgta tatgccgtag cgcatcacgt cgtcgcgcag
    10741 ttgcgcccac atctcacggg taggtaacgt aataccgctg cgggcaaaca gttcgccaac
    10801 tttcgccgtt ttcggctgcc agttaccttg cagatattgg ctgaaatatt caccactggc
    10861 atagcgtgac tgtttgaacc cggcgaaggt ttcaccgcgt tcgcgcgcca gcaacatcga
    10921 ggtacgcagt gcgtgccagg tgatggtata gaaatagaga ttggtgaaat ccagggcttc
    10981 cggcgaacca taagcgatgc cttccctcgc cagataacca tgtaaattca tctgccccag
    11041 tccgatggcg tgcgaggcgg catttccggc ttcgatggac ggcacgctgc ggatatgact
    11101 catatctgac accgccgtta aaccgcgcac ggcagtctct accgtgcggg aaaaatcggg
    11161 ggaatccatg gtgtgcgcaa tattcaacga acctaaattg caggaaatat catggcctgt
    11221 gcgggcatag tcgagattct cgtcatactc tgaggcgctg ttaacctgca aaatttctga
    11281 gcagagatta ctcatattta tgcgcccggc gatagggtta gcacggttta ctgtgtcttc
    11341 atacatgatg tagggatagc cggactcaaa ctggatttct gccagtcgct ggaagaaatc
    11401 acgggcgttg aggtattttt tgcggatgcg ttcatcggca accagttcgt catagtgttc
    11461 gctaatggcg atatcggcaa acggcttgcc ataaactcgc tcaacgtcat agggcgaaaa
    11521 cagcgccatc tgcgcattct cttttgccag atggaaagtg atatccggga tcaccacgcc
    11581 cagcgacagt gttttaatgc ggattttttc gtcggcattt tcccgtttcg tgtcgagaaa
    11641 acgcagaata tcgggatgat gagcatgtaa atagactgca cctgccccct gacgcgcgcc
    11701 gagttggttg gcataggaaa atgcgtcttc caacattttc atcaccggaa ttacgccaga
    11761 agattgattt tcaatacgtt taattggcgc gcccgcttcc cgcagattcg acagcaaaaa
    11821 tgctacgccg ccgccgcgtt tcgacagctg cagtgcggaa tttaccgccc gaccaatcga
    11881 ctccatattg tcttcaatac gcagcaaaaa acaggaaacc agttcgccgc gctgctgctt
    11941 accgcagttg aggaatgttg gcgtggctgg ctgaaagcgt cctgacagca tttcatcggt
    12001 cagttgcaac gccagcgtct catcgccttg tgccagcgtc agcgccacca tcgtgactcg
    12061 atcggcaaaa tcttccagat aacgtttacc gtcgaatgtc ttcaacgtat agctggtgta
    12121 aaacttccat gccccgagga atgtctggaa acgaaaaccg ctggtgtgtg cgtgggcaaa
    12181 cagcgtaatg acaaagtcgc gagagtagcg attaagaacg ctttcatcat agtaaccttc
    12241 gttgaccagc cagttcaggc gctgctgctg gctactgaag gtcacactgt tcggacgcac
    12301 atgcgtcgca ataaaggcgt caacggcctg gcggtcttta tcgaactgaa tgcgacctgc
    12361 gctatcgtaa agattaagca tcgcattcag cgcgtggtaa tccatcgttt cctgcgtcag
    12421 gcattctgcg gttgtcgttg ccaaaattcg gttactcctt tacgaacgtt ttcgatatcg
    12481 ctttgcgtac ccatgagttc aaaacggtac agccacggca cgccgcattt ccgggcaatc
    12541 acatctccgg cgcggccata cgcctcacca aagttgcgat taccagaagc aataacgccg
    12601 cgaagcaacg cccggttgtg ctcgtcgttt aaaaagcgaa ttacctgtcg tggcaccgcg
    12661 ccagccgtac cgccgccgcc gtaagagggc acgatcagga tgtaaggctc gtctacctga
    12721 atccgttccc gctcattgag cgggatgcgc accgcgggca gacctaaacg ttcgataaaa
    12781 cgctgcgtgt tttcggagct gctggagaag tagacgagct ggctcatgca ctggccgctt
    12841 gtggcgctgg atgcagacgg ttaatcatgt ccggacggaa accagaccag ctaagatcgc
    12901 cagcaatcac taccggcaac tgacgaaagc cctgagcacg caacgcttcc gctgcttcag
    12961 gaacgcgatc gacattaatc atttcaaaat caaagccccg gttttccatc gcccgtttgg
    13021 tggcgtggca ctgaacgcaa tcgttacgag tataaatagt tatgcgcatg attcgtattt
    13081 ccgtttaaaa tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct gttgatacag
    13141 agatactaga tgtagttgaa aaaagattca accacacaat atatagcaaa tagtgatcga
    13201 aattacccta gatatgagcg tgacggggga gggggatttt tgtgattcac caggcaaaaa
    13261 gaaaccccga agacaggctt cggggtcaaa gacgcgtatt tattatcatt tttgcactac
    13321 gatttgcgca tgcttaacag tgcgccgata aaaataccta ccgcagctgc tgtacccacg
    13381 ctacaccagg gtctttcacg aacaaaagaa tccgcgcagc caacggcatc gcgcgcggct
    13441 tgctggacgc gagtacgacc atgcattcgc gccctggttt ctttcagcaa cgcctgagct
    13501 ttactgcgtg cggcttcagc ttcccctttg gcgtcgctgc cccaggactt caatacagat
    13561 tcgaggctgt cagctaattg attgacatca ttctggatat cctgcacgcc atcatcaacg
    13621 tcgttgcggt tcggtcggtt aaacatatga tcccccatta gaattcgatg taattttcag
    13681 tgtagaccat aattttctta actgaggttc atcgcggctt tatggacttt tctgaaagcg
    13741 ttgtgaatgc gcaatcaggt aaggtaggcc cgcggttgaa aataacgagg atcaaatatg
    13801 tatttacgac cagacgaggt ggcgcgcgta cttgaaaaag tcggttttac tgtcgatgtg
    13861 gtaacccaga aagcgtatgg ttaccgccgt ggggaaaatt atgtctatgt taatcgcgaa
    13921 gcgcgaatgg gacgtaccgc tctggtaatt catccaacat taaaagaacg cagttcaaca
    13981 ctggctgaac ccgcttccga tattaaaact tgcgatcatt accagcaatt tccgctctat
    14041 ttagcaggcg agcgacacga gcattacggt atcccgcatg gctttagttc gcgtgttgcg
    14101 cttgaacgtt atttgaatgg tttatttggc gaagccagtt aaagacgcga ctggcgatgc
    14161 cagtcgcgaa aagaagagtc aggcttttac ctgctggtaa cggctgactt taaacagtcg
    14221 gcggcaataa tcgaggaagt agccataaac cgcccccatc aacatcgaaa caacgatgtt
    14281 tgaactgacc gccgccataa tctgatgcca gtctgcgccc accactaaca agatcgccac
    14341 atacaccggt gactggaacg tcacataagc caggatatca gccagatttt ttatccagcc
    14401 cgacgggcta actttgcgtg ccgcgcgcat aaacagatcg cggtacatac cgtatggcca
    14461 tgcaattaag atgttcaccg gaatcgctac caatctggaa taaaaagact gttcgaagct
    14521 cattccggag aggaaaactt caatacacat gttcacgaca gaacagtaaa caaccatcgc
    14581 gaacgtatct gcaactgcat gacgcaagcg tgactgcggt gagaacatgc taatgctcct
    14641 taataaaagc gtaatgggat gttaatggag atgaaagcag tggatctcat tgcaatctgc
    14701 attttcgcat agcgtatatc gctggctatg actaatcaat tagtgaaaaa tttttattta
    14761 gtcagttttt gtagataaaa tattccgtaa cggctcgttt tttgtacatt gtggattttt
    14821 acatgctgat atgatttatt ttcaattaaa tcaattggat atggccttgc ctttaatttc
    14881 actaatgaaa gtttgggggt gaggtaacgc tataagcgta gagaattcgg tgtttgtcgt
    14941 gaccgggtca tttacagcat ttttgatgcc agaaaatgta cattaacagc agaaagcacc
    15001 agtgaggggt gttttctttt attctgttat attgcatttt tattcacttc ggttttgtca
    15061 ggcttgcgga gttagcgagc agagagcgcc actctgttca ccaataacct aaattactca
    15121 tcaaaatggc taatatataa tcttgaaatt attctgagag tttcagaaaa taacagctga
    15181 aataatctcg cgcaggactg taaatagatt aaattttgtg gaaatataat aagtgatcgc
    15241 ttacactacg cgacgaaata cttttttgtt ttggcgttaa aaggttttct ttattatgtc
    15301 cgtaatgtta caaagtttaa ataacattcg caccctccgt gcgatggctc gcgaattctc
    15361 cattgacgtt cttgaagaaa tgctcgaaaa attcagggtt gtcactaaag aaagacgtga
    15421 agaagaagaa cagcagcagc gtgaactggc tgagcgtcag gaaaaaatta gcacctggct
    15481 ggagctgatg aaagctgacg gaattaaccc ggaagagtta ttgggtaata gctctgctgc
    15541 tgcaccacgt gctggtaaaa aacgccagcc gcgtccggcg aaatataaat tcactgatgt
    15601 taacggtgaa actaaaacct ggaccggtca gggccgtaca ccgaagccaa ttgctcaggc
    15661 gctggcagaa ggtaaatttc tcgacgattt cctgatctaa ttactgaggc cggatgtcgc
    15721 tgctagggcg cgtccggcgt cgcttctcaa gctcgttggt tccacggcat cttatctaac
    15781 aaccttgcca ttccacaaaa accgctgatt cctgcaaaca gtaacccggc accaacaaag
    15841 ccacttaata agaagaaacc gctatttacg gtataaccca gtacaacgcc gattaatatt
    15901 aaaccacccg cagcgatctg cacctggcgc attaacggca agggttgaga tttatttacc
    15961 gctactggca atccagctct tttccagcca tcaatcccat cttcgagtaa aaatatttct
    16021 gcgggggcgg caatcgctgc taatttatcg gcgttattac tggtgcgttt acctgcctgg
    16081 cagtggaata taatttgctc gcgacgtaat ttaggtggaa gacctgactg ttccagcacg
    16141 gataatggag ccagatctgc ttcaggaata tgttcacgaa gatattcatc agcgtcacga
    16201 atatcgatta actttgcgcc gcgtgcgatt aattcttgtg catcatgcgg cgaaatggtt
    16261 gtcaaagcca tagtgactcc ttacggacaa tagacatttt tcagggtggc gataatggca
    16321 tttaccgcct catttttaat ggaatataga atgcgttggg catcccgttg gctgttgata
    16381 agcccttcgt cccgcatacg agcgagatgc tgtgatgtcg cagaggcgct cagtccggta
    16441 atgcgcgtca gctctcccgc gctggtgccg ggggaaccgc taagcatgca cagaatcagc
    16501 aaccgtttag ggtggctcat tgctttcaat aaggccgctg cctgttcggt actggcctgt
    16561 aattgcgcga gttcagtcat atttatttaa gtattttcta aattaagtaa actctaaact
    16621 aaaaatgcaa catataccag cctcagcagc gtaaatgaga gtaaaagcgt aagctgaaac
    16681 tggcaggctc cgctaaaatt actacgctta agagataaaa tctcttttta aacaatgagt
    16741 aattttctta tagggagtac atatgggttt ctggagaatc gtcatcacca tcattctgcc
    16801 gccgctcggc gtgctgctcg gtaaagggtt cggttgggcg ttcattatta atattctgct
    16861 gacactgctt gggtatatcc ccggtttgat tcacgcgttc tgggtgcaaa cccgcgatta
    16921 aacctgtgct cacccccgcg gcgttaacgc gccgcgttcc tctgctacac tttctgagtg
    16981 tttccgttaa cgaatgaggt agctatgggt ctgttcaatt ttgtgaaaga tgccggagaa
    17041 aaactctggg acgcggttac aggtcagcac gataaagacg atcaggcgaa gaaggtgctg
    17101 gagcatctga gcaaaaccgg tatacctgat gccgataaag tgaacattca aattgccgac
    17161 ggcaaagcga cggtcactgg tgacggcctg agtcaggagg cgaaggagaa aatccttgtt
    17221 gcggtgggga atatttccgg tattgccagt gttgatgatc aggtgaaaac ggcgacacca
    17281 gccactgcca gccagtttta taccgttaag tctggcgaca ctctgagtgc catttccaaa
    17341 caggtctacg gtaacgctaa tctgtacaat aaaatcttcg aagcgaataa accgatgcta
    17401 aaaagcccgg ataaaattta tccggggcaa gtgttgcgta ttccggaaga gtagttaatt
    17461 gccagccatc gcctgctgga taatgggaat tggcgtcagt aaatgctggc gcattaactc
    17521 actggcgcgc gctgtatctc gcgccagtac cgccgcagtc agggtctggt gctgatcgtg
    17581 tttatcctcc agcatttcca ccgaaagcac cgttcgccgc agccagataa atcgataacg
    17641 cgccgccaga tcaaacaacc gttcacgcat ttgcagcaaa tagtgagaac cacagcccgc
    17701 cacaattgct gtatgaaacg cctgatgacg cagatcccac tcatcaagca ttttctcgct
    17761 ggcgtcacag gcttccagct tactgagcag atgcgctttt gcgagaacgt ctgcctccca
    17821 ttcatcgcca ccgcgggcaa tcgccagact gaccagcatc gcttccatat tggcgcgggc
    17881 gtcgaaaata tcgagcagct cctgctctga catagacgcc acccgatacc ctttttgatt
    17941 caccaccgtg accagccgtt ccgccaccag ttgagaaagt gcttcccgca acggcccaac
    18001 gccaagtgca taacgcgatg tcagtaaact catccgtaat ttttcatccg gttgaaaatt
    18061 accgcgaata atatcgttct tcagccagcg atagccatcc agagacgtaa tggtcatggt
    18121 gtgactcctg atcattatca gcgcgtatta tgaacgggtg ttttttgcca caataccagc
    18181 tttttccagc gagccataat tggcacggta cagataatcc ctatcgctaa taagccggtg
    18241 gagatcactt ctaactgttg cgccggacgg aatagcatca ctaccaacac aaaggtaata
    18301 aagccgatga ccagccaggt gagccaggga taaagccaca ttcgcaagcg aatttcgctt
    18361 ccttctgctc gcagaatttt gcgcatccgc aactgtgaaa cggcgatgac taaataaacc
    18421 agcagggcga tagcgccgga gctgtcgatc agaaatttaa ataccttcgc aggcgcgtaa
    18481 tagttcacca ccaccgttaa gaatgccgcg ccggtggaga gtaacaccgc cacgtacggg
    18541 gttttactgc ggttgatttt acccattacc gcgggagcat caccgcgacg gcttaaggag
    18601 tagagcatcc ttgaggcggt atacagggcc gagttcaggc aactggttac ggaaagtaat
    18661 atcacacagt ccatgattaa tttcgcatgg ggaatattga gcaattccag aaccgagcga
    18721 taagatccaa cggctttcag ccccggcata ttccacggta ttaatgccac gacgacaaaa
    18781 atagagcaca aatagaagat agaaatacgc cagataaccg agttggtggc gcggacaata
    18841 tgtttttccg gcgtgtcgga ttccgcggcg gcaatggtga caatttctgc gcccataaac
    18901 gagaacatgg tgatcaacat cgcgcttaat accgcaccga aaccgttggg cataaagccg
    18961 ccgctatccc acaatcttga gatcccgctc acttcggcat aagggtaaat accgctaatt
    19021 gcgactgcac caaggaaaat aaaggccagg atagcgatga ctttgcacag cgccagccag
    19081 aactcaaatt cgccgtagtt tttaacgctt aataaattac tgccagttaa ggcgagggta
    19141 atgacgaggg aaaataacca gatgggaatg cctggaaccc acgagtgcag gatcatggcg
    19201 gcgatgttgg cttccagtgg gataaccagt acccaaaacc accagtacaa ccagccgatg
    19261 gtatagcccg cccagcggcc aatggcttta tcggcatagg tggaaaacga accggtatcg
    19321 ggcgtggcaa ctgccatttc cgccagcatc cgcataatca taaccaccag taatccggcg
    19381 aacagatagg ccagtaatac cgccgggccc gcttcggcga tggcgacgct ggaaccgaca
    19441 aacagacttg cgccgataac accggcaata gacaacatgg tgacgtgacg tgacgtgacg
    19501 tgacgtgacg tgacgtgacg tgacgtgatt tcagcccgcc gcctaactcg tgtggttgcg
    19561 atgattgccc catcctgaat cctctcgaaa gtgtgtcact tgggagagcg ccccgacatt
    19621 tcccgcgtcg ggtcacgcgt ttaattgtta ttgattcttt ttgccggaga gcggctccgc
    19681 cttatccggc cgacatagat ttattcctgt tcacgtacag attgtcggat gcggcgtaaa
    19741 cgccttatcc gacttggatt ctcgtaggcc ggacaagacg cgcagcgtcg cctcctgcat
    19801 gggggcggtg ctactgcttc gcctcatcaa aacactgacc gataatctcc agaccctgac
    19861 ggatctgagc gtcttcaatg gtgagcggta caaggatgcg cagcacgttg taatacgggc
    19921 cgcaggagag aagaatcagg cccttatcgc gggcgcgtgc cacgatctcg gcggtgagtt
    19981 ttgcgtccgg tttgctgtga tcgccatctt caaacagctc gatggcaatc atcgctccca
    20041 gcccgcgtac gtcgccgatc tccgggtgtt tttcggctat cgccaacaat ccgtctttta
    20101 gcttctgccc cagatcgttg gctttttgca gcagattttc ctgctcgaac accttcaaca
    20161 cttccagcgc agccacgcag gcaatcgggt taccggcata ggtgccgccc agaccgcccg
    20221 gtgcaacagc gtccatcact tccgcgcgcc cggtgacgcc cgccagcggg aaaccgcctg
    20281 cgatcgattt cgcaaaggtg gtaagatccg gcgccacgcc catctgctcc atcgcaaaca
    20341 gcgtgccggt acgccccgcg ccgctctgca cttcatcggc aatcagcatg atcccgtgct
    20401 cgtcacacag agcgcgtaaa cgctgcataa aggctggcga cgcggcgtag aaaccgcctt
    20461 cgccctgaac cggctcaatc atgatggcgg cgatatcttc cggcgcggca tcatttttga
    20521 agatccggtg gatgctggcg atagcgtcat cctcgcttat gccgtgcagc gggcaaggat
    20581 aaagcgcgcg ataaacatga cccggcatca gccccatgcc cgcagagtac ggattcacct
    20641 tgccggtcag cgccagcgtg taatgcgtgc gcccgtgata cgcgccgcta aaagcgatgg
    20701 tgccgctacg tttggtggcg gcgcgggcga tttttaccgc gttttccacc gcttcggaac
    20761 cggtcgtaac cagcagcgtt ttcttggcga aatcgcccgg caccttctga ttcataatct
    20821 cgcacagctc cagatacggc tcgtaagcca gcacctggaa gcaggtgtgc gacagttttt
    20881 tcaactgcgc ttccaccgcg gccaccacct tcggatgcag gtgcctggta ttgagcaccg
    20941 caatcccgcc cgcgaaatca agatactcac ggccttcaac gtcccacacc cggcagtttt
    21001 ccgcgcggtc agcgaaaatc gggtgaattt gcccaacgcc acggggaatc gcctgactgc
    21061 ggcgctgcat taactcttta ttgctgttca ttcgcattct ccagttaaag accgatgcac
    21121 atatatttga tttctaagta atcttcgatg ccatacttcg aaccttcacg acccagaccc
    21181 gaggctttga tgccgccgaa tggggccact tcattggaaa taatgccggt attgatgccg
    21241 acgatgccgt actccagcgc ctcgccgacg cggaagacgc ggcttaaatc acgggcgtag
    21301 aaataggcgg caagaccaaa ctcggtgtca ttggcttgtg caatcacatc ggcttcatct
    21361 ttaaaacgaa acagtggggc gagggggccg aacgtctctt ctttcgacac tttggcgttg
    21421 gccggaacgt ccaccagaat ggttggctgg aagaagttgc cgccgcgttc gtgcgcttta
    21481 ccgccgcaaa ccacacgcgc acctttctcc agcgcatcgg caatatactc ttccactttt
    21541 gctaccgctt tttcatcgat cagcggcccg atggtgacgc ctttctccag cccgtcgccg
    21601 atgtgcagtt tgctcaccgc ctgctgtaat ttttcggcaa
//
LOCUS       DADLSE010000058        21395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLSE010000058 DADLSE010000000
VERSION     DADLSE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.058"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            59..931
                     /locus_tag="JN692_002191"
     CDS             59..931
                     /locus_tag="JN692_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7264500.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN692_002192"
     CDS             complement(991..2289)
                     /gene="eno"
                     /locus_tag="JN692_002192"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7264501.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN692_002193"
     CDS             complement(2377..4014)
                     /gene="pyrG"
                     /locus_tag="JN692_002193"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7264502.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN692_002194"
                     /pseudo
     CDS             complement(4242..5033)
                     /gene="mazG"
                     /locus_tag="JN692_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(5129..7363)
                     /locus_tag="JN692_002195"
     CDS             complement(5129..7363)
                     /locus_tag="JN692_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAY7264503.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN692_002196"
     CDS             complement(7411..8712)
                     /gene="rlmD"
                     /locus_tag="JN692_002196"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7264504.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLGALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLQEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8769..11525
                     /gene="barA"
                     /locus_tag="JN692_002197"
     CDS             8769..11525
                     /gene="barA"
                     /locus_tag="JN692_002197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7264505.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN692_002198"
     CDS             complement(11757..13097)
                     /gene="gudD"
                     /locus_tag="JN692_002198"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7264506.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13118..14458)
                     /locus_tag="JN692_002199"
     CDS             complement(13118..14458)
                     /locus_tag="JN692_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7264507.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14460..15812)
                     /locus_tag="JN692_002200"
     CDS             complement(14460..15812)
                     /locus_tag="JN692_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264508.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKSVAGQ
                     "
     gene            complement(16246..16695)
                     /locus_tag="JN692_002201"
     CDS             complement(16246..16695)
                     /locus_tag="JN692_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7264509.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN692_002202"
     CDS             complement(16713..17495)
                     /gene="truC"
                     /locus_tag="JN692_002202"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7264510.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17495..17824)
                     /locus_tag="JN692_002203"
     CDS             complement(17495..17824)
                     /locus_tag="JN692_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7264511.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN692_002204"
     CDS             complement(18446..18991)
                     /gene="syd"
                     /locus_tag="JN692_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7264512.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19059..19907
                     /gene="queF"
                     /locus_tag="JN692_002205"
     CDS             19059..19907
                     /gene="queF"
                     /locus_tag="JN692_002205"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7264513.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20019..21383
                     /gene="ppnN"
                     /locus_tag="JN692_002206"
     CDS             20019..21383
                     /gene="ppnN"
                     /locus_tag="JN692_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7264514.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5257 a         5701 c         5104 g         5333 t
ORIGIN      
        1 tctaaaatta ccgatgccgc cgtcaattta tttatccaga tttaatgaga aatttataat
       61 gcgctatttc atactaatgt tcactttcgt atgttccttt gttgcagccc aaccaactat
      121 tgtcccacaa ttacagcaac aagttacggg tcttacgagt agcttaaact cacaagaaaa
      181 gaaagaactg acgcacaagt tagaatctat tttcaataat acccaagtgc aaattgctgt
      241 attaattgtt tccacaacca aagatgagac aattgaacaa tatgctacaa gagtttttga
      301 caattggcgt ttaggagatg ccaaacgtaa tgatgggata ctgatcgttg ttgcctggtc
      361 ggatcgcact gtccgcatcc aggtaggtta tgggctggaa gaaaaggtaa ccgatgctct
      421 ggctggagat atcatccgta gcaacatgat acccgcattt aaacaacaaa aattagctaa
      481 gggattagag ttagctataa tcgctttgaa taaccaactc acttcacaac accaatatcc
      541 ggctaatcct tcagaaagtg aatcaacgtc ttccactgat cattattact ttgctatttt
      601 ttgggtattt gcagtgatgt tcttcccttt ctggtttttt catcaaggca gtaatttttg
      661 tcgcgcatgt aaaagtggag tctgtatttc agcaatccat cttttagatt tattcctgtt
      721 ctccgataaa actttttcca ttgctgtatt ttccttcttt tttactttca ccatatttat
      781 ggtctttacc tgtttatgtg tacttcagaa aagagcatct ggtagaagtt atcattcaga
      841 caacagcggt tccgctggag gctcaagtgg cggcggattt agcggcgggg gcggttcttc
      901 tggcggtggc ggcgcatccg gccgctggta actccaccat aaaaaatgcc agcccgaagg
      961 ctggcattat caaatcagat aaagtcagac ttatgcctgg cctttgatct ctttacgacc
     1021 gttgtacggt gctttttcgc ccagagcttc ttcgatacga atcagctggt tgtatttagc
     1081 aacacggtca gaacggctca tagaaccagt tttgatctgg cctgcagcag taccaacagc
     1141 cagatcagcg atggtagcat cttcagtttc accagaacgg tgagagataa ctgcagtgta
     1201 gccagcatct ttcgccatct tgattgcagc cagagtttcg gtcagagaac cgatctggtt
     1261 gaatttgatc aggatggagt tagcgatacc tttttcgata ccttctttca ggatcttggt
     1321 gttggttacg aacaggtcgt caccaaccag ctggattttg tcgcccagaa ctttggtctg
     1381 gtatgcgaaa ccgtcccagt cagattcgtc cagaccgtct tcgatagaaa cgatcgggta
     1441 ctgtttggtc agttcttcca ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc
     1501 ttcgccagcc agaacatatt taccatcttt gtagaattca gaagctgcgc agtccatcgc
     1561 cagagtgatg tctttgccca gttcataacc agcagcttta acagcttcag cgataacagc
     1621 cagagcttca gcgttggaac ccaggttcgg cgcatagcca ccttcgtcac caacagcagt
     1681 gttcatgcct ttcgctttca gaacttttgc caggtgatgg aaaacttcag aacccatgcg
     1741 gatggcttct ttcacagttt tcgcgccaac cggctgaatc atgaattcct ggatatcaac
     1801 gttgttgtca gcgtgctcac caccgttgat gatgttcatc atcggaaccg gcatggagta
     1861 tttgcccgga gtgccgttca gttcagcgat gtgctcgtac agcggcatac ctttagcagc
     1921 tgcagcagct ttggcgttag ccagagatac agccaggatt gcgttcgcgc cgaatttgga
     1981 tttgttttcg gtgccgtcca ggtcgatcat gatcttgtca atgccagcct gatctttagc
     2041 atctttgcca atcagcgcct gagcgatcgg gccgtttacc gcagcaacag ctttggttac
     2101 gcctttaccc aggaaacggg atttgtcgcc atcgcgcagt tccagagctt cacgggaacc
     2161 agtagaagca cctgacggag cagctgccat accgacgaaa ccaccctcca gatgtacttc
     2221 ggcttcaaca gtcgggttac cacgggagtc gatgatttca cgaccgatga tttttacgat
     2281 tttggacatt aggttttcct caagtcacta gttaaactga aactccagac aaacaacgcg
     2341 tacccagggt acgcgttgcc gttctaactt ttttacttac ttcgcctgac gtttctggaa
     2401 ctcgctggcg gctttcacaa agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt
     2461 aaactccgga tggaactggc aagccacgaa ccacgggtga ttcggaactt cgatgatctc
     2521 gaccaactga tcatccccgg aacggcccgc aacgcgcaga cctgcatctt caatctgttt
     2581 caacagcatg ttgttgactt cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt
     2641 gtatagctgg cgaaccaggc tatcgtcaac caactggcac tgctgtgcgc cgagacgcat
     2701 ggtaccgccg agatcgctct tctcgctacg aacttcaacg ttgccgtttt catcgcgcca
     2761 ctcggtaatc agcgccacaa ccgggtactt acagtctggc acaaattctg tagagttggc
     2821 gttctccatg ttggcaacat ggcgagcgta atcaattaac gccacctgca tacccaggca
     2881 aatgcccaga taaggaatat tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc
     2941 tacgccacga tagccgaaac cgccaggtac gaggattgcg tccagacctt taaggatttc
     3001 aacgccgcgc gtttcaacat cttgtgaatc gatcagtttg atgttgacgc tgacacgatt
     3061 cttcagccca ccgtgtttca gtgcttcgat cactgattta taagcatccg gcagttcaat
     3121 gtacttgccg accataccga tggtgacttc acttaccgga ttcgcttctt cgaagataac
     3181 ctgttcccat tcggacagat ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata
     3241 atcgtccagc ccctgagatt tcaacaggcc cggaatttta tagatggaat cgacgtcttt
     3301 cagagaaata accgcttttt ccggaacatt acagaacaat gcaatcttcg cacgttcgtt
     3361 cgccggtaca gcgcgatctg aacgacaaat caggatgtca ggctggatac cgatggagag
     3421 cagctctttt acagagtgct gagtcggttt ggttttgact tcaccagacg ctgccatgta
     3481 cggcaccagc gtcaggtgca taaacagagt gtgctcacgg ccaatttcaa cagccatctg
     3541 gcgaatagct tcgaggaacg gcaaggattc gatatcacct actgtaccgc cgatttctac
     3601 cagtactacg tcatgacctt cgccaccttc cagcacgcgc tctttgattg cgttagtgat
     3661 gtgcggaata acctgcacgg ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac
     3721 gtcagagtag atacgacccg tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa
     3781 acgctcgtag tgccccaggt ccaggtcggt ttcagcgccg tcttcagtaa cgaacacttc
     3841 cccgtgttgg attgggctca tagtacctgg atcgacgttg atgtaaggat ccagtttcat
     3901 gatggtcaca ttgaggccac gggcttcaag aatggctgcg agggaggctg cggcaatgcc
     3961 tttacccaga gaggatacga ccccgccggt cacaaaaata tagttcgttg tcatgctgaa
     4021 cctgagaagt taggttgaaa agacgatgga ataaccaaga cgggaaagca gtatacccga
     4081 acatgacctg tgccacaaac tttcattatc cctcctcttc gccagcgcac tattgaaatc
     4141 aggagtgaga aaatagcccc tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca
     4201 tttaaaaaat gacacaaatg gcgcttgacc gcgtaattcc cttagagatc aatttcctgc
     4261 cgttttacct gttgccagac ttcttccatt gtttcgaggt caacacctgt catttccagt
     4321 ccacgcgcgg caacaatacg ctccacttcg cgaaaacgac gctcgaattt ttcgttcgct
     4381 ttttgcaatg cgatttctgc tttcgtccct aaatggcgag ccagattaac cgtggcaaac
     4441 agcaggtccc ccatttcctc ctccagttta gcctggtcga caacagcctg ccgcgcctcg
     4501 tacatcacct cgtcgatctc ttcgtagact ttatcaacca ccgggccaag cgtcgtccag
     4561 tcgaagccaa cgttggcgca acgtttctgg attttttgcg cccgcattaa agccggtaaa
     4621 ctacgaggaa tatcgtccag cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg
     4681 atttgctccc aacgggcaag cacttcacta ctgttttcgg cagaactatc agcaaaaaca
     4741 tgcggatggc gacgctctaa tttatcgcta atagcagcgc aaatatcatt aaagtcaaag
     4801 cgcccttctt cctgagccat ttgcgcgtaa aacaccacct agaatagcag atcgcccagt
     4861 tcaccgcgaa gatcgtcaaa atcttcacgg gcgatggcgt ccagcacttc gtaggtttct
     4921 tcaagggtgt aaggcgcaat ggtggcaaat gtctgctctt tatcccacgg gcagccgttt
     4981 tccggatcgc gcaggcgctg cataatagtg agcaaacggt cgatttgatt cattgaattg
     5041 tcctgaaaat tgcgggtctg tcaggtgtaa taccttcgag taaaagatat cgctggatac
     5101 ggctgacgcc atatccagct atgtaagact aactcccgtg caaccgacgc gcgtcgataa
     5161 catccggcac ctggttgagt ttacccagca cgcgccccag cacttgcagg ttgtaaatct
     5221 caatggtcat gtcgatggtc gccagttgct gtttggtgtc gctacggctg gcaacgccaa
     5281 gcacgttcac cttctcgttg gcgagaatgg tcgtgatatc acgtaacaac ccactacgat
     5341 cattagccac cacgcggacc accagcgaat atccggcgga gtagctctca ccccataccg
     5401 cgtcaacaat gcgttctggc gcatgggagc gcagttccgc cagttgttcg caatcggcgc
     5461 ggtgtactga aataccgcgt ccctgggtaa tgaagccgac aatctcatct ccaggaatcg
     5521 gctggcagca gcgcgcgatg tggtgcatca ggttaccaac accttcaacc accacacgac
     5581 cgttatcttt actgcggttt tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg
     5641 cggcgtcctg ctcttcggca ctcggcttat taaattgcga ttgcaggaag ttcaccatct
     5701 gattgagacg gatatccccg ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt
     5761 tgtaacgcgg cagcagatgt ttttctgctt ctttcaggct gatccccaga tgttccagct
     5821 cgtcgtcaag gatttgccgc ccagccagaa tgtttttgtc acggtcctgt ttacggaacc
     5881 aggcgtgaat tttcgaacgc ccacggctgg ttgtgacgta accgaggttt gggtttaacc
     5941 agtcacggct ggggttcggc tgtttctggg tgataatttc aatctggtcg cccatttgca
     6001 gctggtaggt gaacggcaca atgcgcccgc caatttttgc cccgatgcag cggtgtccga
     6061 catcactgtg gatgtggtaa gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga
     6121 catcaccttt cggcgtaaag acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt
     6181 cgagcatttc gccggaatca gccatctctt cctgccacgc aatcagttta cgcagccagg
     6241 caatccggtc ttcatgtccc gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc
     6301 agtgcgcagc aacacccaac tccgcatctt catgcatctg tttggtgcgg atttggatct
     6361 caacggtttt tccacccggc cccagaacca cggtatgaat agactgataa ccgtttggtt
     6421 tcgggttagc gacgtaatcg tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta
     6481 tccccagtgc ggcataacaa tcctgtaaac gctcggcgac aatacgtacc gcacgcacat
     6541 caaacagctc atcgaaggcg aggttctttt tctgcatttt gcgccagatg ctgtagatgt
     6601 gtttcggacg accatacact tcagctttaa cgccttcagc tttcatctca gcgcgcagat
     6661 ggccgacaaa ctcttcgata tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg
     6721 cgatgcgttt gtattcggtc gggtggagat aacggaagca gtaatcttcc agctcccatt
     6781 tcagttgccc aatcccaaga cggttcgcca acggcgcgta gatattggtg cactcttttg
     6841 ccgccagtac acgttcatct tccggcgcat cttttacttc gcgcagatga gcaatacgct
     6901 ccgccagttt gatgactacg cagcgaaaat catcgaccat cgccaataac atccggcgaa
     6961 cgttatcgac ctgttcggag gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg
     7021 ccgccatatc acgcacgccg tgaataaggt taacaaccga cttaccgacg ctctcacgca
     7081 gcacatcttc gctgactacg ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg
     7141 tgtcaatgtc catacttaat gtcgagagga tctccaccat ctcaacacca cgccacaata
     7201 acagactggc atccggatgc ccctgcgtct gttgcagaca atacgcccag gtttcggcta
     7261 agcactcaca cgacttctgg ctggtaatac ccagacttgc gatccatttt tccggatcaa
     7321 attcaccagc cttattgata tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg
     7381 accagacctg ccgaaatcgg caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga
     7441 ttccagatgt cccgtgtgtg ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc
     7501 tgcttttaat aacgcttcgc tatcccgagc cagcgttgca gggttacagg atacataaac
     7561 tatacgaata ggttccagtt ttataatttg ctgcataaca cctgcggcac ctgctcgcgc
     7621 cgggtccagc aacactttat cgaagccgtt tttcgcccac ggctgctttg tgacatcttc
     7681 ttgaagattt tcgtgataaa acgtcacatt ctgtaagcca ttaagacgcg cattctgctg
     7741 gcctttttcc accagcgccg gaacaccttc tacaccgacc acactggcag cttgtgtcgc
     7801 caatggcagt gtaaagttgc ccataccgca gaacagatcc agtacgcgat cttcaggttg
     7861 cacatccagc cattccaacg cacgcgctac cattttttgg ttcacacccg cattgacctg
     7921 aataaaatcg cgcgggctaa aagttaagcg caacccgttt gagtcatacc agggcatctc
     7981 accagagacg gtttcgagta tctcactatc gggggcgaga tacagatcca ggccttcaga
     8041 atgcgaaaag cgttccagtt tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa
     8101 aatcatcagc gtgccgctgg ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc
     8161 ttgtaagctg cccagacatg ccctgacttt gggcagcaat gctccaagtt ggggcgctaa
     8221 aatggggcat tgtttgacgt cgacaatgtc actggagccc gctttgcgaa accccatctg
     8281 aagttgctgt gttttcggta agtagttcag acttaaacgc gcgcggcggc gatagcccca
     8341 gggaacatcg gcgatcactt cagagacatc gtgtttcatt aatcgggcga gtgccgcact
     8401 tttgcttcgc tgctgtaaat ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc
     8461 aaaatgagga cagcgtggcg tttcgcgttc cgggctatcg cttaaccggc gtacgacttt
     8521 agcgcgggca tactgttttt tatcttcagt aacagcaact tccgcgtttt cctgcggcaa
     8581 taatccgggg ataaataacg ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc
     8641 gaggtcgttg actgaaacgg ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta
     8701 gaattgcgcc attggcgaga ctttctcaat ttaacagtgt gaccttaatt gtcccataac
     8761 ggaactccat gaccaactac agcctgcgcg cacgcatgat gattctgatc ctggcaccga
     8821 ccgtccttat tggtttattg ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc
     8881 agcgtcaact ggaagatgcc ggtgctagca ttattgagcc gcttgcagtt tctactgaat
     8941 atggcatgag cctgcaaaat cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc
     9001 gccattccga tattgttcgc gcgatttcgg tttatgatga aaataaccga ctctttgtca
     9061 cctccaattt tcatcttgat ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc
     9121 gccagctcac tgtcactcgt gacggcgata ttatgatcct ccgcacgccg attatttctg
     9181 agagttactc ccccgacgaa tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg
     9241 gatatattgc gcttgagctg gatcttaaat cggttcgctt gcagcaatat aaagagatct
     9301 ttatttcctg cgtgatgatg ctgttttgta tcggtattgc gcttattttt ggctggcgct
     9361 taatgcgcga tgtaaccggt ccgattcgca acatggtgaa taccgtcgac cgcatccgtc
     9421 gcgggcaact cgacagccgg gtggaaggat ttatgctcgg cgagctggat atgctgaaaa
     9481 acggtatcaa ctcgatggca atgtcgctgg ctgcttatca cgaagagatg cagcacaata
     9541 tcgaccaagc gacgtccgat ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg
     9601 agttggatct ggcgaaaaag cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg
     9661 caaatatgtc acacgagctg cgtacaccac tgaatggtgt tattggcttt acccgcctga
     9721 cgctgaaaac agaattaaca ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg
     9781 caaataattt gctggcaatt attaatgatg ttctcgactt ctcgaaactg gaagcaggta
     9841 agctgattct ggaaagtatt ccattcccac tacgcagcac gctggatgaa gtcgttactc
     9901 tgctggcaca ttcttctcac gataaagggt tggaactgac gctcaatatt aaaagcgacg
     9961 tgcctgataa cgtgatcggc gacccactgc gattacagca aatcatcact aacctagtgg
    10021 ggaatgcaat taaattcacc gagaatggca acattgatat tctggtagaa aaacgtgcgc
    10081 tgagtaatac caaagtgcag attgaagtgc agattcggga taccggcatt ggtattcctg
    10141 aacgcgatca atcgcgctta ttccaggcct tccgacaggc tgatgccagt atttcccgcc
    10201 gtcatggtgg caccgggctg gggctggtga ttacacaaaa actggttaat gaaatgggcg
    10261 gcgatatttc gttccatagc cagccgaatc gcggttcaac tttctggttc cacattaatc
    10321 tcgatctgaa cccgaacatt attatcgaag ggccatccat ccagtgcctc gcaggtaagc
    10381 gcctggccta tgtcgaacca aactccgcag cagcgcaatg cacgctggat attttaagtg
    10441 aaacgccgct ggaagtggtt tatagcccaa cgttctccgc gctgcctccc gcgcattacg
    10501 acatgatgtt gttaggcatc gcggtgacct tccgcgagcc gctaacaatg caacatgagc
    10561 gattagcgaa agcggtatcg atgaccgatt tcctgatgct ggcacttcct tgccatgcac
    10621 aagtcaatgc tgaaaaactc aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa
    10681 cacctacgcg cctgttgcct gccctgacgg aattttgtca tcacaaacaa aacacgcttt
    10741 tgcctgtaac cgatgaaagt aagctggcaa tgacagtcat ggcggttgat gacaaccccg
    10801 ctaacctgaa acttatcggc gcattgctgg aagatatggt gcaacatgtg gaactttgcg
    10861 atagcgggca tcaggcagtt gaacgggcga aacagatgcc gttcgatttg atcttaatgg
    10921 atattcaaat gccggacatg gatggcattc gggcctgcga actcatccac caactcccgc
    10981 atcagcgaca aacgccggtt atcgcggtaa cggcgcatgc aatggccggg caaaaagaga
    11041 agctgcttgg cgcagggatg agcgattatc tggcgaaacc gattgaagaa gagcgattgc
    11101 ataatttgtt gttgcgctac aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg
    11161 aagtcaacga aattgtggtg aacccgaatg cgaccctcga ctggcaactg gcactacgcc
    11221 aggcagcagg aaaaaccgat ttagcgcgcg atatgctgca aatgttactc gatttcctgc
    11281 ctgaagttcg caacaaagtt gaggaacagc tggttggaga aaacccggaa ggcctggttg
    11341 atttgattca taaactgcat ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc
    11401 tctgccagct gatcgaacaa cagctacgta gcggtactaa agaagaagat ttggagccgg
    11461 agctgctgga actgttggac gagatggata atgtcgcgcg cgaagccagc aaaattctcg
    11521 ggtaacggac gtcttgtcga gtagcaatcg gctttttatg acgctggttt cagattggtg
    11581 acaaagtgcc tgtgtttatg ccggatgcgg cgttaacgcc ttatcctgcc tacaaaacca
    11641 attaaattca atgaattaaa aaatgatgta gacctgatac atagcacaac aggctatttt
    11701 gcgtttagca tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa
    11761 cgcaccatgc acgggcgctt gttatcgaac gtccagccag gaatcagata ctgcattccc
    11821 atcgcatcgt cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc tttcatcact
    11881 tgatccatat cgatttctac acccagcccc ggtttttctg gcacctgtac cagcccgcct
    11941 ttgatctcaa acggttcttt ggtcaggcgc tgattacctt cctgccagat ccagtgcgta
    12001 tcaatagcag taattttacc cggtgcagcg gcggcaacat gggtaaacat cgccagggaa
    12061 atatcgaagt ggttgtttga gtgtgaaccc caggtcaggc caaattcatg gcacatttgc
    12121 gccacacgta ccgaaccttg catagtccag aaatgcggat ccgccagcgg gatatcaacg
    12181 gattgtaacg agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgca
    12241 gtcggcaggc cagtcgcgcg acggaactca gccatcactt cacgcccgga gaaaccttgc
    12301 tccgcaccac acggatcttc tgcataagcc agcgaacctt tcaggtattt accaatttta
    12361 attgcttcgt tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag
    12421 cgttgcgcca gtgccacaat agactcggct tcttcttccc cggccagtac accgcctttc
    12481 agtttgaaat cgttgaagcc atatttttca tacgccgctt ccgccaggcg caccaccgca
    12541 tccggcgtca tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg
    12601 ctttgatacg gcagaggtgt ggctttgcga ttaccgacga agaacagata accgagcatt
    12661 tcgacttcgc tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc
    12721 tgccccagca gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc
    12781 aaatcaaatg tctgcaaacc gcgcccacta gcatcacgat cggcaaaagt attacgcacc
    12841 agcgtcagaa cgtttttgta ttcacccagc gttttaccta ccaccagcgg aatcgcatct
    12901 tccagcgttt tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa
    12961 ttatctttga taatcacaat attacgcgtg aagaacggtg cgtgtgcacc actcagattc
    13021 atcagcatac tgtcatgacc cgccaccggg atgacctgca tttcagtaac aacaggcgtc
    13081 gtaaattgag aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac
    13141 gtttacggtc aaaggtccag ccggggatca ggtactgcat cggacctgcg tcgttacgcg
    13201 caccgcaagg cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt
    13261 ccacgcccag accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat
    13321 ttttggtcag gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat
    13381 tacccggtgc cgccgcgccc acatgggtaa acatcgccag agagatatcg aaatggttgt
    13441 tggaatggca gccccaggtc agcccccagt cgtcgcaaag ctgcgccacg cggactgcac
    13501 cggaaagcgt ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg
    13561 catgacccat ttcgcgccag ttggtggcga tcatgttagt cgcgacgggc aagccggtcg
    13621 cccgtcgaaa ctccgccatc acttcacgtc cggagaagcc ctgttctgcg ccgcatggat
    13681 cttcggcata ggtaagaaca tcattcagcc ctttgcataa agaaatggct tcatcaagca
    13741 gccatgcacc gttgggatca acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac
    13801 gaacagtgtc gatttcttgc tcgccaggta acaccccgcc cttaagtttg aaatctttaa
    13861 agccatagcg atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt
    13921 tctgatggcg caactgatac cactcatggt tgcccggcgt attttccaga taaggaagat
    13981 cggttttggt ccgatcaccg atataaaaca gataaccgag gacggtaata gtctcgcgtt
    14041 gcttgcctgg ccccaacagt tcgcagaccg gaacattcag cgccttacct agcaggtcaa
    14101 gcaaggcggc ttccagcgcc gccacggcat taacgcgcaa ttcaaaagtc caggcacctt
    14161 tgccgaaggt atcaaaatcg gctgcctgat tacctttatg cacctgctga accactttat
    14221 tcaggcgcgc aacttcctgg cccagaacca tcggaatagc atcgaccagt gtttgataaa
    14281 tcacctctcc gcccggcgct tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca
    14341 cgatattgcg ggtgaagtat gcgttatgcg caccaccaat attgagcaac atgctgtcat
    14401 gtccggcgac cggaatgact ttcatatcag taataacagg actggattgt gtcgccatct
    14461 tattgccccg cgacagattt cagctcgata cgcttgatat cgcccaccag caccaggtag
    14521 ctcagtaccg cgattagggc atgaacacca acataaatca gcgccccatt aaacgagcca
    14581 gtcgtgccaa cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccgaac
    14641 atgttgaaca ggccaccgga aagaccgctg atctcttttg gcgcggtatc tgccattact
    14701 gcccaaccca gcgccccgat gcccttaccg aagaaggcca gcgccataaa gccgatgatc
    14761 atccactcaa cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatt
    14821 acgatcggtg ttttacgcgc aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa
    14881 ataattccac ccagcacacc gccgataaaa ccgcaaaccg ccggaacgga agccacaaag
    14941 cccgctttca gaatcgacat cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg
    15001 aaaaagtaag tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt
    15061 gacccgagca gctgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg
    15121 ttttgctgat ccatattgat cagcgcgcca cccgcggcga tatactccag ctctttctga
    15181 tttacccccg gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca
    15241 atgcccagac cgcccataaa gaagaagacg tgtgaccagc ccacttcatg cgtcagccag
    15301 cccataatcg gcgcgaagat caccgttgcg aagtattgag cggagttaaa aatcgacacc
    15361 gccgttcccc tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa
    15421 gatggcgctt cagcaagccc gaccaggaag cgcagagtaa acagggcaac gataatgccg
    15481 aatccactaa agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc
    15541 gaccagaagt agacgcgttt tgaaccaaaa cgatccagca accaaccacc agggatctgc
    15601 ccgataacat aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg
    15661 ccgatatctt tggccatttc cgaaccggca atagaaagcg tagctcggtc gccgtagttg
    15721 aaggatgtga cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc
    15781 acactgctcg cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc
    15841 cacgctcact ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgttttttg
    15901 ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa tagcagtata
    15961 aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc
    16021 cgattttggc attagcccta gacggtggaa tccacttcac ggaaatgaaa acaagaacaa
    16081 gaaaggaagg gtaaaaacga agaaataaag aagagtatga aatggatcgc ttgactctag
    16141 gcaaacgcca gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg cctgaaaaga
    16201 ttcgattcag caccgctaaa acgacattta ccgctcgctg acatatcagg acaacagcgt
    16261 gccccactgt tcgacccacg gatttgattc cgtttccggt tccgggttct cactggcgtc
    16321 aatcaacagc atttcgccaa cccgctgagc gctctgttcc tgcaacaagg catcgaactg
    16381 tttgccgcca ttgcaaaaat ttacataact gctgtcgccg agcgcaatca cgccataacg
    16441 cagattcggc tggaagccca gactatcttt gattccctga aagagcggca caatgctatc
    16501 aggaaggtcg ccctgcccgg tcgtggacgt aaccaccaga acatacttat cctgataggg
    16561 cagccagtcg cttaattcag gatcttcaaa taccgttgct ttgtggccct gtgcggtcag
    16621 aattgcttct gcttcttccg ccactaacag cgaatttccg tacatggtgc cgacaaaaat
    16681 accaatttcc gccataccgt tttctccctg gattaggaac ttatctctcc atcctgaccc
    16741 gatggcgcac tgaactcaac cctttcattt tcaggaagca gaccgcgcca gccaaattgt
    16801 gataacgcct gcatccaggt gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta
    16861 aaaggatgcg tcaatgacaa ctgactggca tgtagcatta accgctggag gccaaaatgc
    16921 tcagcaccgc tgcgattctg gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga
    16981 cgcaaatggg caagatgtcg gcgaagctga tgtttgcgtc cggttttcgg ctccagttcc
    17041 accaggccgt agcgcgtggt cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc
    17101 agaccgcgat aatgcgtcac tgctggctgc gggcctttat cttcgcgggc aaatttatca
    17161 gcgattttgt ccagttcttc caccagtgga taatccaaca ccgcttcttc catcaaccag
    17221 ccgcgcacaa tcgcatggta acgtttctgg atttggtgct gttcaaactg ttgtgccagc
    17281 agccgtccgg cctcgctgga taatcccatc aacaacacac cagaagtggg tcggtccaga
    17341 cgatgagcag taaaaacatg ctgtcctatc tggtcacgca cggtttgcat gaccactact
    17401 ttctcgtcgc gatccagcca gctacggtga accagccagc cggagggttt atttaccgca
    17461 accagccatt catcctgata gagtatttcc agcattagct cgcatcatcc gcaaaaagag
    17521 catccagttt ttccaactca gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg
    17581 ccatttcata atagggtgca acagcaaaag cgcccggtaa cggctggttg ttatttaaca
    17641 aatcgtgcat tcgcgggatc agcccccact gcaaccactc cagtggttcc atggtgtcca
    17701 taaagaacgg ttgggtacta ttaaattgat gcggctgggg ttcatcgttt cgccagtgct
    17761 gatgttcacg cagtaacgct tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg
    17821 tcataaagca acctcaataa gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga
    17881 gcactgtatt cacagtgctc ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc
    17941 ctgctcatcc ttgacaactt ttcctctggc cttgcggcca atcgttcatc ctgaactatt
    18001 gcttcctgct cacaccaccc cgatgtgata cttcatcctg aagtgtccct ggccttcctg
    18061 acccaccgaa tcatcctgac cggttctcat tctccatcct ggaggtgtcc tttaacgcgt
    18121 cctgcgtcat cctcttcgct tcatccagaa gcctttccct gaaacaccat cctggtgtgt
    18181 cctgcagaag tgtcatcatc ctgatgttca cttcgttgtc tgactccctg tcgacgaaga
    18241 tagaatcgtc tttttcgcga agtcttacaa ggcgcttaca gacaatgcct taaggaaaat
    18301 ttcatatgaa agttaagaat taacaatcaa cattttgatt ttaaatgaaa ataacgattc
    18361 ttatcttttt aagtattcgc ttatttcctg gcgatctcct acagagtctg taagagatct
    18421 ctcacaacca gatggggtat ttggattaca gaagaggctt aagttgatta aggaattccg
    18481 caagatttga ggctaaatgc gtccgtttac gtgttcccag cgtctctttg cacacttcac
    18541 cggagagatt acacaccgag atgacctcta actcttcttc cagcgtcgcg ataaatagcg
    18601 tgggcggcag ttttagcctt ttctgagtca ccagatgacc aatcagattt tcctgcaccc
    18661 gacggaagtc gtcttcactc caggtttgca gcaatgtcag tttgatatcg ccaaactgcg
    18721 cgtgcatatc cccggcaaac tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa
    18781 tatcaaaagc gcgttcaacc gcgtttacat tttgttcccc ggtaaacggc tggggttgcc
    18841 agtatacggc atcttcggta gttgaaataa tgcacggtga aggaacaccg tacagttcct
    18901 cacttaacgg ccagctttta tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat
    18961 ctttcagggc ttgtgcggtc aaatcgtcca ctaatttctc tcttcactta aaccagatac
    19021 acttgtcgtt tagtttatct ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc
    19081 aggcacttgc gggcctgact cttggaaaat caaccgatta ccgggatacc tatgacgcca
    19141 gcctactgca aggcgttcca cgcagcctga atcgcgaccc gctgggtctg aaagcggata
    19201 acctgccttt tcacggtacg gatatctgga cgctgtatga actttcctgg ctgaatgcga
    19261 aaggtttgcc gcaggtcgct gtcggtcatg ttgaacttga ttacaccagc gtaaatctga
    19321 ttgagtcgaa gagttttaag ctctatctca acagttttaa ccagacgcgt tttaataact
    19381 gggatgaggt gcgccagacg ctggagcgcg acttaagcac ttgcgctcag ggtaagatta
    19441 gcgtggcgtt atatcgtctt gatgaactgg aaggccagcc gataggtcat ttcaatggca
    19501 cttgcattga tgaccaggat atcactatcg ataactatga attcactact gactatctgg
    19561 agaatgccac cagcggtgaa aaagtagtgg aagagacgct cgtcagccac ctgctgaaat
    19621 caaactgcct gatcacccat caaccagatt ggggttcgat ccaaattcag tatcgtggac
    19681 gccaaattga cagagaaaaa ctgctgcgtt acctggtctc attccgtcat cacaacgagt
    19741 tccacgaaca gtgcgtggaa cgcatcttta atgacctgtt acgcttctgc cagccagaga
    19801 aattgagcgt ttacgcacgt tatacccgtc gtggcggtct ggacattaac ccgtggcgca
    19861 gtaatagcga ttttgtccca tcgaccacaa gactggttcg gcaataaatt ttttctcaat
    19921 tttgcgtgct ggattcacgc agaaggttgt gaaaggtcat caggcagggc tattgtaatc
    19981 aaagggaatg acgatattcg tcccataagg agtttttctt gattacacat attagcccgc
    20041 ttggctccat ggatatgttg tcgcagctgg aagtggatat gcttaaacgc accgccagca
    20101 gcgacctcta tcaactgttt cgcaactgtt cacttgccgt actgaactcc ggtagtttga
    20161 ccgataacag caaagaattg ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc
    20221 gtgaacgcgg cgtaaagctg gaactgatta atcccccgga agaggctttt gtcgatgggc
    20281 gaattattcg cgctttgcag gccaacttgt tcgcggttct gcgagacatt ctcttcgttt
    20341 acgggcaaat ccataatacc gttcgttttc ccaacctgaa tctcgacaac tccgtccaca
    20401 tcactaacct ggtcttttcc atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc
    20461 caaatatggt ggtctgctgg ggcggtcact caattaacga aaacgagtat ttgtatgccc
    20521 gtcgcgtcgg gaaccagctg ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag
    20581 gagcgatgga agcgccgatg aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag
    20641 acagtcgttt tattggtatg acagagccgt cgattatcgc cgctgaaccg cctaacccgc
    20701 tggtcaacga attgatcatc atgccggata tcgaaaaacg tctggaagcg tttgtccgta
    20761 tcgctcacgg catcattatc ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt
    20821 tgctgggaat tttaatgaac ccggccaaca aagatcaggt tttaccattg atcctcaccg
    20881 gcccgaaaga gagcgccgac tacttccgcg tactggacga gtttgtcgta catacgctgg
    20941 gcgaaaacgc gcgccgccat taccgcatca tcattgatga cgccgctgaa gtcgctcgtc
    21001 agatgaaaaa atcgatgccg ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca
    21061 gctttaactg gtcaatgcgc attgcgccag atttgcaaat gccgtttgag ccgtctcacg
    21121 agaatatggc taatctgaag ctttacccgg atcaacctgt tgaagtgctg gctgccgacc
    21181 tgcgccgtgc gttctccggt attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca
    21241 ttgaagagtt tggtccttac aaaatcaacg gcgataaaga gattatgcgt cgtatggacg
    21301 acctgctaca gggttttgtt gcccagcatc gtatgaagtt gccaggctca gcctacatcc
    21361 cttgctacga aatctgcacg taatctcccc tcccc
//
LOCUS       DADLSE010000059        21264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLSE010000059 DADLSE010000000
VERSION     DADLSE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.059"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            14..922
                     /locus_tag="JN692_002207"
     CDS             14..922
                     /locus_tag="JN692_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264515.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1161..1220
                     /locus_tag="JN692_002208"
     CDS             1161..1220
                     /locus_tag="JN692_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7264516.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1537..1596
                     /gene="ibsE"
                     /locus_tag="JN692_002209"
     CDS             1537..1596
                     /gene="ibsE"
                     /locus_tag="JN692_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7264517.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN692_002210"
     CDS             complement(1716..3149)
                     /gene="hldE"
                     /locus_tag="JN692_002210"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7264518.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN692_002211"
     CDS             complement(3197..6037)
                     /gene="glnE"
                     /locus_tag="JN692_002211"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7264519.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDVAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SRLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVSARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6060..7361)
                     /locus_tag="JN692_002212"
     CDS             complement(6060..7361)
                     /locus_tag="JN692_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7264520.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVMAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7603..8223
                     /locus_tag="JN692_002213"
     CDS             7603..8223
                     /locus_tag="JN692_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7264521.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8287..9525
                     /gene="cca"
                     /locus_tag="JN692_002214"
     CDS             8287..9525
                     /gene="cca"
                     /locus_tag="JN692_002214"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7264522.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN692_002215"
     CDS             complement(9688..10509)
                     /gene="bacA"
                     /locus_tag="JN692_002215"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7264523.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN692_002216"
     CDS             complement(10600..10968)
                     /gene="folB"
                     /locus_tag="JN692_002216"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7264524.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11073..11690
                     /gene="plsY"
                     /locus_tag="JN692_002217"
     CDS             11073..11690
                     /gene="plsY"
                     /locus_tag="JN692_002217"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7264525.1"
                     /translation="MSAIALGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN692_002218"
                     /gene_synonym="dan"
     CDS             complement(11703..12635)
                     /gene="ttdR"
                     /locus_tag="JN692_002218"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HAY7264526.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            12842..13685
                     /gene="ttdA"
                     /locus_tag="JN692_002219"
                     /pseudo
     CDS             12842..13685
                     /gene="ttdA"
                     /locus_tag="JN692_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13682..14287
                     /gene="ttdB"
                     /locus_tag="JN692_002220"
     CDS             13682..14287
                     /gene="ttdB"
                     /locus_tag="JN692_002220"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7264527.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14335..15798
                     /locus_tag="JN692_002221"
     CDS             14335..15798
                     /locus_tag="JN692_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7264528.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLLIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN692_002222"
     CDS             complement(15841..16854)
                     /gene="tsaD"
                     /locus_tag="JN692_002222"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7264529.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17092..17307
                     /gene="rpsU"
                     /locus_tag="JN692_002223"
     CDS             17092..17307
                     /gene="rpsU"
                     /locus_tag="JN692_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7264530.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17418..19163
                     /gene="dnaG"
                     /locus_tag="JN692_002224"
     CDS             17418..19163
                     /gene="dnaG"
                     /locus_tag="JN692_002224"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7264531.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19358..21199
                     /gene="rpoD"
                     /locus_tag="JN692_002225"
     CDS             19358..21199
                     /gene="rpoD"
                     /locus_tag="JN692_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7264532.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5118 a         5881 c         5465 g         4800 t
ORIGIN      
        1 acaagaagcg tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca
       61 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
      121 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
      181 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga
      241 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
      301 agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac
      361 cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
      421 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
      481 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
      541 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
      601 cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt
      661 tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
      721 cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctag cggaacaagc
      781 attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga
      841 aattggcgtt tcaggcggtt cactggcggc attgacttca cccttaacct caacaactcc
      901 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctagcaca aaaagccggg
      961 gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg
     1021 cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct
     1081 catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg
     1141 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg
     1201 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     1261 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     1321 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
     1381 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
     1441 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     1501 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     1561 gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat
     1621 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc
     1681 ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     1741 ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg
     1801 ccgttggccc agacttcttt acttccggcg atctcttctg gtttatagtc gccccccttc
     1861 accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac
     1921 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     1981 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     2041 aagcggtcac ccagtttgcg ggcatttgcc agataggaga cgtgtccggc gtgcaggatg
     2101 tcaaagacgc cattggtcat caccactttt tcaccgcgtt tacgcgctgc cgctacggcc
     2161 agcttcagtt cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca
     2221 ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc
     2281 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     2341 aggacgccaa tcaccgtgtc gcctgcaccg gtaacgtcat acacttcctg cgcctgggtt
     2401 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     2461 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     2521 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg tgtcagcagc
     2581 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc
     2641 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
     2701 gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc
     2761 ggatcaacac cttcgaaacc ttcttcaaaa tccagacgga tcagctgttg gttgcgggaa
     2821 agtacccgta atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg
     2881 ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg
     2941 accaggcgtg cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc
     3001 ggacgttctt cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacga
     3061 ctggtggggc cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     3121 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     3181 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat
     3241 cacgctctgc ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat
     3301 gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt
     3361 ccataatgtc gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta
     3421 acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg
     3481 taattccgcc ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag
     3541 cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tctcgcggca
     3601 gtgtcataat ctcacgacgc accgcgtcaa agtgcgcagt gagctgcgga tcgccgtaca
     3661 ctacacgcgc actcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat
     3721 cggcaaatgc ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgag
     3781 catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt
     3841 gcgccagacg cagataaaac tgccgcccgt cgatttcccg ctcgccgtca gtcatcgcat
     3901 ccattgggca atcatggagg aagataaggt caagatcgga actgtagcct aactcccagc
     3961 cgcccagctt gccgtagccg accaccgcaa aaccgcgccc ttcacgttcg ttgaggtgat
     4021 ttggcttacc gtagcgggcc accatttgac cccacgcctg ctgaacgacg gcatctatca
     4081 tagcttctgc cagccaggtt aagtgatcgc tcactttcat taccggtagc gtaccggcga
     4141 tatccgctgc cgcaatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
     4201 gttgttcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcagttca tcacggtagg
     4261 catcggtcgc cgtcggctgg taaagggtat ttggatcgag caattcatcc agcaataatg
     4321 gataacgcgc tagccggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt
     4381 taagcgccgc agggaattca ctgagcaatt ctaaataggt tgtgcgggta acaatcccca
     4441 ccagcaaggc ggtaatgcgc gacagcgtga cagcaacgtc ttcacgcgca cagacatcac
     4501 ttagcagatg tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct
     4561 tatccagctc tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc
     4621 taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac
     4681 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
     4741 taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt
     4801 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt
     4861 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg
     4921 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atggcgctga
     4981 gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga
     5041 acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg
     5101 tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa
     5161 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt
     5221 tagcatagac gccttcgcta tcgcccataa tccgcgcttt gaccatcgcg taacgctccc
     5281 agtcgcgtcc ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc
     5341 cactttcgcc aaacggacgc agtcgcatat ccacgcgata gacgaagcca tcctgcgttg
     5401 gttgatccag cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
     5461 gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat
     5521 cagaggagaa attcagttcc ccgccgccca gcttacccat gcctaagatc agcagcggtt
     5581 gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaggcg tcatacagcc
     5641 agtcacgcgc cgcaacaatc agcgtctccg ccagataact gagctgctgc aatatgctct
     5701 cttcagtaac cagtgccagc gtttgcgccc atgcgatgcg caccataatg cgccgccgga
     5761 atagccgcag ctcgcgcatt aacccggctt cgtcgctcac attactgagc gcctcctgca
     5821 accacacgct gtaatgttgc cattcgtcag cctgtggcgg ttggctttcc agttccgtca
     5881 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
     5941 tcgcctgtgc actaagtgat tcctcggcta aaagctctgg cagccgctca acaacggtct
     6001 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
     6061 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
     6121 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
     6181 caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca
     6241 cgcatcaggc gtggcaactg gtcacggtag cgatcgccta acggctggca gaaaacgctt
     6301 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggagtcgcta
     6361 attttgccct gcgctttggc atctaaaaac ggctgccatg ctttgcttac cagccattcg
     6421 gtcaacgcca gcttcgccat tgccgtttcg gtagagtaga ccgccatcac ggcagaaacc
     6481 gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgcttta
     6541 cgcggcacaa tgccaccgaa cagcatcagt gtatgacgga ctaggctaat ggctgccagc
     6601 acctgctctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
     6661 gctaacacca gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca
     6721 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
     6781 cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
     6841 agtttcagca ccgcgcgcgt gtcgccgcta agcagctcca gttccagctc gcagataggc
     6901 tcagcaaatt cacccgcttt cacttccccc tggtcgagag cgatttcaat tcggctgcca
     6961 tcgatctcta ctagccattt ttcgcgataa aaatcggtgc taaacagcgg ctgcacgcgg
     7021 gaagcgagat cggcaggcaa ttcgccgttc ggccagattt ccgtcggtaa ctgcgccagg
     7081 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
     7141 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag
     7201 cccatatcgt gcctacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc
     7261 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca
     7321 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta
     7381 tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta
     7441 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
     7501 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
     7561 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
     7621 atcggattaa ctttactcgc acttagcgcg actgccgtct cacacgctga agaaacgcgc
     7681 tatgtttctg acgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
     7741 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
     7801 tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc
     7861 actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc
     7921 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
     7981 gtggcgcaga gcgacagcgt gatcaacggg ttaaaagaag aaaatcagaa actgaaaaac
     8041 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag
     8101 cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
     8161 ctcggtctgg tactgccgca cctgatccca agccgcaaac gcaaagatcg ctggatgaac
     8221 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
     8281 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctatcg
     8341 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
     8401 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
     8461 gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc
     8521 gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
     8581 gcccaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
     8641 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt actgcgcgtg
     8701 gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg
     8761 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
     8821 aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc
     8881 gattgcggcg cacttcgcgt tttattcccg gaaattgacg cactgtttgg cgttccggca
     8941 cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
     9001 gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt
     9061 aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt
     9121 aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
     9181 agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc
     9241 atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg
     9301 gcgctgacca gcgaggctga cgtgcgcggc agaaccggtt ttgaatcagc ggactacccg
     9361 caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc
     9421 gttgaagcgg gatttaaagg cgtggagatt cgcgaggagc tgacccgacg gcggattgcg
     9481 gcggtagcca gctggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg
     9541 ttagcacgaa ccgtcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca
     9601 gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac
     9661 ccggcctgct cccgaacctt ttctcaatca aaagaacacg acatacaccg cagccgccac
     9721 aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt
     9781 tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat
     9841 atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat
     9901 catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag
     9961 catcccacct gaaatggtcg ccccggaacg ggagaaaccc ggccacagcg ccagacactg
    10021 gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg
    10081 ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagcgcata
    10141 catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaataata gccccagtac
    10201 caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttcacc
    10261 ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa
    10321 catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt
    10381 gtcgccctca aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg
    10441 cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat
    10501 atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgaa ctactgcagt
    10561 atgaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt ctttcagatt
    10621 attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc ctggcttgct
    10681 gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg ccacctcttc
    10741 agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca ccgtttctgc
    10801 aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt tacggttatc
    10861 ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc agtcgtaaac
    10921 accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca cgtcctgcct
    10981 gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    11041 gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgctt ggaatgatcc
    11101 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ttgtgtgggc
    11161 tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg ttacgtatcg
    11221 gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcctg
    11281 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    11341 cctgtcttgg acacatctgg cccgttttct tcggttttaa aggaggaaaa ggcgttgcta
    11401 ccgcttttgg agcaatcgca ccaattggct gggatctcac cggagtaatg gcgggaacct
    11461 ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    11521 ttgctccgtt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    11581 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    11641 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    11701 atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca ccatgccgcc
    11761 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    11821 cagatattgg cgctttgtgc atactccggc aatacttgca ccagtttgcc actctccaga
    11881 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    11941 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    12001 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    12061 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    12121 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    12181 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    12241 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    12301 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    12361 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    12421 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    12481 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtt
    12541 tgcccaagcg tcgctgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    12601 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    12661 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    12721 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    12781 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    12841 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    12901 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    12961 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    13021 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    13081 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    13141 cgtggaatag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    13201 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    13261 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    13321 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    13381 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    13441 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgcc cgattggcag
    13501 ccgccatccc aatccaaaag cggcagaact ggagctacgc ctggaagaag gactcaaccg
    13561 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg catcgtcgcg
    13621 gcacgctgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt
    13681 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    13741 cgatgtgatc tacctgaccg gtacgctggt gacctgccgc gacgtttgtc accgccgttt
    13801 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    13861 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtc tccgtcggcc cgacaaccag
    13921 tatgcgtatg gaaagttttg aacgtgaatt tattgagcag accggcgtaa aactggtggt
    13981 tggcaaaggt ggtatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca
    14041 tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtagaag agattgaaga
    14101 agtgcactgg acagaactcg ggatgccgga gtcactgtgg gtctgccggg tcaaagagtt
    14161 cggcccgctg attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa
    14221 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat
    14281 caaataaccc tcccggagag gctcacccct ctcctttttc gcaggcataa cacgatgaaa
    14341 ccttccactg aatggtggcg atatcttgcg ccgctggcgg tcatcgccat tattgctcta
    14401 cttccggttc ccgcagggct ggagaaccat acctggctct actttgccgt ttttactggc
    14461 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    14521 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    14581 ggctttaagt tcaccgccaa atccctctcc tgggcagttt ctggtttttc taattcggtt
    14641 atctggctca ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggcttgga
    14701 cgccgcattg cgctgattct ggtgaaaaag atggggcatc gcacgctgtt tctcggctat
    14761 gcggtgatgt tctccgagct gatcctggca cctgtaacac cgtccaactc ggcgcgtggt
    14821 gcgggtatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    14881 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    14941 gtgaccagcg ccattttcct gacggcgatg gcacctaact tgctgttaat tggactgatg
    15001 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    15061 agcattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccggtactg
    15121 aagtctggtg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    15181 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    15241 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    15301 ctgctcatta tcagttggga cgacattgtc agtaataaag cggcatggaa cgttttcttc
    15361 tggctggcct cgcttatcac cctcgcgacc ggactcaaca ataccggttt tattagctgg
    15421 tttggcaaac tgttagcagg cagcttaagt ggttattcgc cgacgatggt gatggtgacg
    15481 ttgattgtgg tgttttatct actgcgctac tttttcgcca gcgccacggc gtatacctcc
    15541 gccctcgcac cgatgatgat cgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    15601 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    15661 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggcggatta ctggcgactg
    15721 ggggcgattt ttgggctgat attcctcgtg ttgctggtga ttaccggctt actgtggatg
    15781 cccgtggtgt tgctttaagt gagggcggtg ccggaaattg ctctccggca cccaatagtt
    15841 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acgctaacgc cgagatccgc
    15901 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    15961 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttcagccag
    16021 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    16081 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    16141 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    16201 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    16261 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    16321 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    16381 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    16441 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    16501 cgctaacaga tgcccttcca tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc
    16561 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    16621 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    16681 caacggtacg gttttacgca catgatcgcg ggaggccagt tcaggcacga cgccgccgta
    16741 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    16801 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    16861 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    16921 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    16981 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    17041 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    17101 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    17161 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    17221 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    17281 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    17341 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    17401 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    17461 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    17521 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    17581 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    17641 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    17701 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    17761 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    17821 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    17881 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    17941 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    18001 gcagttacga tcgtttccgc gaacgggtga tgttccccat tcgcgataaa cgcggtcggg
    18061 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    18121 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    18181 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    18241 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    18301 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    18361 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    18421 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    18481 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    18541 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    18601 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    18661 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    18721 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    18781 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    18841 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    18901 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    18961 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    19021 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    19081 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    19141 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    19201 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    19261 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    19321 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    19381 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    19441 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    19501 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    19561 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    19621 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    19681 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    19741 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    19801 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    19861 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    19921 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    19981 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    20041 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    20101 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    20161 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    20221 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    20281 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    20341 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg caaaaactgc agcagattga
    20401 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    20461 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    20521 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    20581 taacatcggc ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    20641 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    20701 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    20761 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    20821 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    20881 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    20941 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    21001 ggctggcctg accgcgcgtg aagcaaaagt tctgcgtatg cgtttcggta tcgatatgaa
    21061 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    21121 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    21181 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    21241 tctctctgca caaacgccac cttt
//
LOCUS       DADLSE010000060        21035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLSE010000060 DADLSE010000000
VERSION     DADLSE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.060"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN692_002226"
     CDS             complement(<1..584)
                     /locus_tag="JN692_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7264533.1"
                     /translation="MNTTNGYNLALQRQLVDSKINDISDLKQKLEFSKIGSASDGMSV
                     TSTVEECEKHGKYTSYEKYLTISGKRITSSKSECPQCLEEKIRKKEIECEQAEQRARQ
                     SKIEYLLNSLNIPERFKNCTLQNYEPVNDDAKRVLKVCQAYASKWPERLQKGGGLVMC
                     GKPGTGKNHLALAIARHAIIEHQSSVIFTTALKIA"
     gene            complement(587..1453)
                     /locus_tag="JN692_002227"
     CDS             complement(587..1453)
                     /locus_tag="JN692_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264534.1"
                     /translation="MARSRNIKPGFFTNDELAECSPYARLLFAGLWTIADKEGRLDDR
                     PKKIKAMVLPFDDVDCNDLLQQLHSHKFINRYQVNGGSYIQINNWKKHQNPHCKEAAS
                     EIPEPVENNNSTMQVQCKDDKDEEKEDVESLQFIENNEAPEKHHASTVQEPVENSLNP
                     ADSLNLIPDSLSNTQAADATCEGGGADVHKISSRYAFEGNIVRLNHKDYEAWKRLYPN
                     IDLNYELERLDIEFSHEKPKNWFITASQKLSYQNKQALSRPVRKVANGLKAENFAAKD
                     YGQTEMPSWAQE"
     gene            complement(1465..2265)
                     /locus_tag="JN692_002228"
     CDS             complement(1465..2265)
                     /locus_tag="JN692_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7264535.1"
                     /translation="MAKKPTPDQIKKIRSGITKKIRFEVFKRDGFKCQYCGSSAPDVI
                     LHVDHINPVSKGGDNDMMNLITSCDSCNGGKSDKLLSDNSIMEKQRQQLQELNTKREQ
                     LEMMIKWRDGLKSLKDDVVDIVATKIDDCIAPFTVNDNGRKSIKRWLRIYKLEEILDA
                     IEIAADKKLTQEITHELTGEFFEYIPRIAATKRKPPKEQRILYIRGILKNRIYINQNH
                     VMSYLKAWLSYDLDLDELTEFAKTVPNWTTFKEWVSERIREAQKELPY"
     gene            complement(2287..2586)
                     /locus_tag="JN692_002229"
     CDS             complement(2287..2586)
                     /locus_tag="JN692_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264536.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(2725..2949)
                     /locus_tag="JN692_002230"
     CDS             complement(2725..2949)
                     /locus_tag="JN692_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7264537.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGQVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3094..3768
                     /locus_tag="JN692_002231"
     CDS             3094..3768
                     /locus_tag="JN692_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7264538.1"
                     /translation="MRSAGVSQSQLADMVGISQPAIQKMSSGKTNGSRKMVELANALK
                     VRPEWLSSGIGEMRDGTHEEPSNIRESSLKAVVWEDIKKNDDEFVALPLLNVSLSAGS
                     GSCEIEESSEFSLVFRKYYLKKMGVTESSAKLVRVTGQSMEPTLHDGDVVGVNTQDTT
                     IRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEFHKTVRIIGRVF
                     WSSHSW"
     gene            3806..4147
                     /locus_tag="JN692_002232"
     CDS             3806..4147
                     /locus_tag="JN692_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HAY7264539.1"
                     /translation="MAFTDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRADPDCCFFG"
     gene            4679..4870
                     /locus_tag="JN692_002233"
     CDS             4679..4870
                     /locus_tag="JN692_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264540.1"
                     /translation="MAPKKKAVADVQVGVKTFELPIPLRGDLIVTIGNLPRDLTKDEA
                     KRISLIVESFAMIDGGTKE"
     gene            5675..6058
                     /locus_tag="JN692_002234"
     CDS             5675..6058
                     /locus_tag="JN692_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7264541.1"
                     /translation="MTRRTQFKGNSRSRRRERLKAKALANGVLTREEAISSEVLHRPT
                     LSRAQIQAKGTHETPERIEDAKPIKFMAQDVIWQQKEYRRNLERAAIVYANEFGHKQP
                     ETGVCLPNVALYAAGYRKSKQLTAR"
     gene            6120..6743
                     /locus_tag="JN692_002235"
     CDS             6120..6743
                     /locus_tag="JN692_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264542.1"
                     /translation="MSSIRLTTRIKEVIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLSSRLMAISKELKKLGCSFYSCDVLSTSIYLAVSGRSVGWYSYG
                     KDSNGEDIWLPTPTRDKCMFDAGHEITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
     gene            6849..6989
                     /locus_tag="JN692_002236"
     CDS             6849..6989
                     /locus_tag="JN692_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY7264543.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            6982..7095
                     /locus_tag="JN692_002237"
     CDS             6982..7095
                     /locus_tag="JN692_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY7264544.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYKKWRSK"
     gene            7092..7280
                     /locus_tag="JN692_002238"
     CDS             7092..7280
                     /locus_tag="JN692_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264545.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            7289..7945
                     /locus_tag="JN692_002239"
     CDS             7289..7945
                     /locus_tag="JN692_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7264546.1"
                     /translation="MGTATLILGESGTGKSTSLRNLPPEKTLIIQCINKPLPFPSSDW
                     VKISKENNNGNIYRTDNTENIVRALINAPHEIVVIDDYQAVMVNELMGKCNERGYDKF
                     TSIGKNAWDVFRAAGEGADTRRVYILAHTQTDDFGYTRMKTVGKLVDSTLVPEGYFTI
                     VLRTHVQNGKYSFSTQTNGSDCCKSPVGMFDSQFIDNDLYEVDKKICSYYGFEKNETS
                     "
     gene            7932..8537
                     /locus_tag="JN692_002240"
     CDS             7932..8537
                     /locus_tag="JN692_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264547.1"
                     /translation="MRRLEVSGERFGNLVVIGDAGMRGTQRLVNCVCDCGVVCVKQLG
                     NLRSGHTRSCGCSRSSTTKARKNKHGMYGTPTYKSWSSMLTRCFNERNSKYKHYGGRG
                     ITVCERWLDFKLFFADMGKRPDGTTLGRIDNNGNYEPSNCEWQKSIEQARNKSNTVLF
                     KFNGVLATISEHCEKTGLNPSTVRSRIYTYHWSVDKALSKG"
     gene            8542..9129
                     /locus_tag="JN692_002241"
     CDS             8542..9129
                     /locus_tag="JN692_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAY7264548.1"
                     /translation="MSNVIFTYNEEAALTAGQGGFINETGAHIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCVGIKQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKSEGKQPETI
                     ANMVASLKDKDNRAKNVSQNHADDYGYSQNDYPPF"
     gene            9153..9449
                     /locus_tag="JN692_002242"
     CDS             9153..9449
                     /locus_tag="JN692_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7264549.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLENIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISND"
     gene            9460..9624
                     /locus_tag="JN692_002243"
     CDS             9460..9624
                     /locus_tag="JN692_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7264550.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFGSVNDNKY
                     LNAMWRSGKK"
     gene            9645..9974
                     /locus_tag="JN692_002244"
     CDS             9645..9974
                     /locus_tag="JN692_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001476141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HAY7264551.1"
                     /translation="MDGFLKGKCIPRDLKVNETNAEYLVRKFDEVRAEARNEGINYTA
                     SRLAAAFNHGFINKSLREVFDVTRMILSAKEELANEQHPIDGLSGGYAEKSLEEWAEQ
                     IRKGGNQ"
     gene            9971..10474
                     /locus_tag="JN692_002245"
     CDS             9971..10474
                     /locus_tag="JN692_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7264552.1"
                     /translation="MSKIDYQALREAAEQATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQEN
                     EEIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVT
                     VKEVGDA"
     gene            10547..11161
                     /locus_tag="JN692_002246"
                     /pseudo
     CDS             10547..11161
                     /locus_tag="JN692_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            11286..12068
                     /locus_tag="JN692_002247"
     CDS             11286..12068
                     /locus_tag="JN692_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7264553.1"
                     /translation="MNTQISVETLSPITYKQTNVITSELLAQLYGTDVVRIRQNHNEN
                     KSRFVEGVHFYKVVGDELRALKHKVALNYFVKIAPNARHLILWTERGAARHAKMLETD
                     QAWDVFEKLEDGYFNQREKVANHPQPVPQPESSLDRARAMEIALNVADRLVNMFPNLS
                     SESKQAAVAGVFNPIVGRDVVPLPAISEHYYSATEVGQRYGISANKVGRIANTYMLKT
                     EKYGKWFIDKSAHNDKQVETFRYNEAGVHKIEELIEGGRKAA"
     gene            12394..13050
                     /locus_tag="JN692_002248"
     CDS             12394..13050
                     /locus_tag="JN692_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7264554.1"
                     /translation="MATQISVESLSPITYNHIPVITTELLARLYGTETNNIKVNYTRN
                     AGRFVCGKHYFKLEGGELREFKNKVTHSNLVAPRAKHLILWTERGAARHAKMLETDQA
                     WEVFEKLEDCYFSPGKAEQHPQIQQQFTDEEIILLCYMQVQMENALDICKRLYPILKE
                     LNSSYTSKLYDIAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF
                     "
     gene            13135..13353
                     /locus_tag="JN692_002249"
     CDS             13135..13353
                     /locus_tag="JN692_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7264555.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            13331..14401
                     /locus_tag="JN692_002250"
     CDS             13331..14401
                     /locus_tag="JN692_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7264556.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            14536..15819
                     /locus_tag="JN692_002251"
     CDS             14536..15819
                     /locus_tag="JN692_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7264557.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15960..18140)
                     /locus_tag="JN692_002252"
     CDS             complement(15960..18140)
                     /locus_tag="JN692_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7264558.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(18323..19756)
                     /locus_tag="JN692_002253"
                     /pseudo
     CDS             complement(18323..19756)
                     /locus_tag="JN692_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            complement(19832..20884)
                     /locus_tag="JN692_002254"
     CDS             complement(19832..20884)
                     /locus_tag="JN692_002254"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7264559.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         6029 a         4801 c         4930 g         5275 t
ORIGIN      
        1 gcaattttca acgcagtggt aaatatcaca gagctttgat gctcgataat ggcgtgccta
       61 gcgatagcca gtgcaagatg gttctttcca gttccaggct taccacacat gaccagtccg
      121 ccaccttttt gaaggcgttc aggccactta ctggcatatg cctggcatac cttaagaact
      181 cgctttgcat catcgttaac aggctcataa ttctgaagag tgcaattttt gaacctctct
      241 ggaatattga gagaattcaa caagtattca atttttgatt gtcttgctct ttgttctgcc
      301 tgctcacatt caatctcttt cttacgaatt ttctcctcaa ggcactgtgg gcactcagat
      361 ttacttgaag taattctttt tcctgagatg gtcagatatt tctcatagga agtatattta
      421 ccgtgtttct cgcactcttc cactgtgctg gtaactgaca ttccatctga tgctgatcca
      481 attttgctaa actcaagttt ctgtttcaga tcagaaatgt cattgatttt tgagtctaca
      541 agttgccttt gcagtgccag attgtaacca tttgtcgtat tcatattcac tcctgcgccc
      601 atgaaggcat ttcagtttgt ccgtaatctt tggcggcaaa gttttcagcc ttaagtccat
      661 tagcaacctt gcgtaccggt ctggataacg cctgcttgtt ctggtagctg agtttctggc
      721 tggcagtgat aaaccagttc tttggcttct catggctaaa ttcgatatcc agcctttcca
      781 gttcgtaatt caggtcaatg ttcggataca ggcgcttcca tgcttcgtag tccttgtggt
      841 ttaaccgaac gatatttcct tcgaaggcat agcgacttga aattttatgg acatcagccc
      901 cacccccttc acaagtcgcg tcagcggctt gggtgttaga aagggaatca ggaatcaggt
      961 taagggaatc agcaggattt aaactgttct ctactggttc ttgcactgta cttgcatggt
     1021 gcttttctgg tgcttcattg ttttcaatga attgcagtga ttctacatcc tccttctctt
     1081 catctttatc gtccttgcac tgtacttgca tggtgctgtt attgttctct actggttctg
     1141 gtatctcact ggcagcttct ttgcagtgag ggttctggtg ctttttccag ttattgattt
     1201 ggatatatga gccgccattt acctgatatc ggttgatgaa tttgtgactg tgaagttgct
     1261 gaagcagatc attgcagtcc acatcatcga aaggcagtac cattgcttta attttctttg
     1321 ggcgatcatc caaccgccct tctttgtcag caatggtcca taacccggca aaaaggagac
     1381 gggcatatgg agaacactca gctaattcat cattggtgaa aaagcctggt ttgatatttc
     1441 ttgaacgtgc cattttgtcc acctttagta cgggagttct ttctgagctt cacgaatgcg
     1501 ttcagatacc cactccttga acgttgtcca gttaggtact gttttcgcaa actcagtaag
     1561 ttcatcgaga tctaagtcgt atgaaagcca tgccttgaga taactcatta catggttttg
     1621 attgatatat atgcggtttt taaggattcc tctgatgtat agaatccttt gttcttttgg
     1681 cggttttctt tttgtcgctg ctatgcgagg gatgtactca aagaattcac cagtaagctc
     1741 atgggttatt tcttgggtga gttttttgtc tgcggcaatc tcaatcgcat cgaggatttc
     1801 ttcaagctta tatattcgaa gccatctctt gatagatttt cgcccattgt cattaacagt
     1861 aaatggcgca atgcagtcgt caattttggt agcgacaata tcaacaacat cgtctttcag
     1921 gctcttaaga ccatctcgcc acttgatcat catctccagt tgctctcgct tggtattaag
     1981 ttcctggagt tgctgccttt gcttttccat tattgagtta tcgctgagca gtttgtcgct
     2041 tttcccgcca ttacaactat cgcaagaagt aataaggttc atcatgtcgt tatcgccacc
     2101 cttgctaact gggttgatat ggtcgacatg aaggatgacg tctggcgcag aactcccaca
     2161 gtactggcat ttgaagccat cgcgcttgaa aacctcaaac cgtattttct tggtaatgcc
     2221 ggaacgaatc ttttttatct ggtctggtgt aggttttttt gccataatta actccgtaga
     2281 aagcagttaa aattccattt gtatttgttc agaacgctcg gtcttgcaca ccgggcgttt
     2341 tttattggtg agtccatcaa gcgcatactt aaaagccctg ctaatcggac tgatgtctga
     2401 tgccattccg aaagcacaca agaccgaagc aataaaccgc cagtcggtac gactgatctt
     2461 cgattcatga cagccaatca tctttgccag accgcgctgg gtaagcgttg acaggttgat
     2521 gagtaaatct gtttctgcac gatcaacgtc gcgctgtgat agtttgctgt aacttgtttg
     2581 ttccatttct taagatttcc aatagtgaat agttagttga aagctatgcg tggaaacgca
     2641 tatggcctta gttggtcaga tatcttgggg ctcgctttgt cagcgacgta ggacgaatgc
     2701 ccattgtgaa aatagcggtg ttacttatgc tgcctgattc ggttttggaa acaggtgtgg
     2761 taaatctggg cgaatttcgt aagccttgat ctgccctcca gtggcgttaa cgatggcggt
     2821 aactttctct ggagaaacct gcccgccttt cagccatttg tgtactgctg gctgcgttac
     2881 accacacttg tcggcaagcc gcttttgact accgacaatc ttcaaggctc gttgaattac
     2941 taaattcatg agcatacctc tttcagccat tacttataac caaggataac ccaagttata
     3001 aaagatagca ataaccttta ttattttact ttggataacc gtagttatag atttgtggat
     3061 atgaaaacat tcgcagaaag actaaatgca gccatgagat cggcaggggt gtcgcaatca
     3121 cagcttgcgg acatggttgg aatatctcag ccagccatac agaagatgtc atccggtaaa
     3181 acaaacggat ctcgcaagat ggttgagtta gctaatgctt taaaagtacg ccctgaatgg
     3241 cttagttctg gtattggtga aatgagggat ggtacacatg aagaaccatc caatatccgt
     3301 gagtcatctt taaaagctgt ggtatgggaa gacattaaaa aaaacgatga cgagtttgtc
     3361 gcattgcctc ttcttaacgt ttcgctttcg gctggaagcg gtagctgcga gatagaagaa
     3421 tcatcagagt tctctttggt tttcagaaag tactatctga aaaagatggg ggtaactgaa
     3481 agttctgcca agctggtaag ggtaacaggt caaagcatgg aaccaactct tcacgatggc
     3541 gatgttgttg gtgttaacac acaagatacc accatcagag atggtaaaac ctacgctatt
     3601 tgccagtctg atttgttgcg agtaaaaaca ttaatcgcca ctcctacatc ggtgataatc
     3661 agatcaataa atcgcgaaga gtacccggat gaagtaatgg atagagatga atttcataaa
     3721 accgtaagga ttattggcag agtattctgg tcatcgcata gctggtaacc agtggcctga
     3781 agaaacgttt gggtgaggag gatagatggc gttcactgac cttgaatatc aagcggtcaa
     3841 aaaagaagtt caccaattca ttgaaagcat aaggccgcct gaacatatcc gcaatgaact
     3901 ggatattgtt tatagcatca atgaccaaac gatagatatc ggcgaacagc gccccgtgtg
     3961 gcagggcaac ccaggtgaaa caaacatcct gccatcagca agaatcaagt acatacgttc
     4021 tctggataga tggaaaatct attggatgcg gaaggatatg aaatggcatc agtacagtac
     4081 tgaactttcg ctgactgatg cgcttgagct tgtgcgtgct gacccggatt gctgcttctt
     4141 cggatgagtg aagagacatt ttggtgatgg atagtcgcag aagtgcggcc tttaatgtaa
     4201 tcgaagaaaa gtaatccttg taaaacttga tttgactgta ttgtcacaaa gaaagaaatc
     4261 aacgatcgtt tcttgggtct tgatttgttt tgacgatcaa attagtattg cctcatcaac
     4321 tgtatgaggc ttttttatgc aaaccaaaag ctacaccatc aaagcatttc aggacttctt
     4381 acagatgctt cctgagcttg gtatcgtcaa tgatgctaca gcccgcaatc tccgtgactc
     4441 ttccctgcgc cttcttactg ttcttgggtc ggatttcacc gatgccgata tccgtgatta
     4501 tagcgttgcc aacttagcaa gttcgtacgc agactcagca gaaagcaaac cctctgaatc
     4561 atctttgcag gcatacaaaa gccggatgca aagtgctatt gataaattta tctcctacca
     4621 gaatggtgag attaatgtgt cagttggtga tgtaaataaa aaaaaggagc gtaaaaagat
     4681 ggcacctaag aagaaggcgg tagctgacgt acaggttggt gtaaaaacat ttgaactgcc
     4741 gattccattg cgcggagatc ttatcgtcac cattggcaac ctgcctcgcg acctgacaaa
     4801 agatgaagcc aagcgtatca gtctgattgt ggaatctttt gcgatgattg atggcggaac
     4861 aaaagaataa agccccgagg gaacggggct ttttgggtcg gaaatatatc cagtatttcc
     4921 gaagtgtatg gaggattacc taccgctaca cccttggcaa ccgcaagcca aggatagcgg
     4981 ttctcccaac aaactacaac ctgttcgggt aaacaggctg tatacaaaaa caggagaaaa
     5041 gctatggctc acgatgttaa tcgcgatgac ttcatctacg aacacgacaa gccggtttat
     5101 gtgcgcagtt actgccgcat acgtttcggt gagctcgaac atgttcgtca gcacttccgc
     5161 tcttatccga gctgctgaaa ataactaaat ctcaaacccg gcttccgcgc cgggttttct
     5221 ttgcctcgcg atcgtccccc atatttaaca gccgcaaatg cggtaaacca cgaaccaaca
     5281 cgccagcaat acagcgcccc ccttccaata ccgaccaatc cgcaacattt acaaaaataa
     5341 aataccttta ttatccatca cttataactt atttatcaga atccataaat taagttattg
     5401 accaccgcta taacctaggt tatctttaag ccatcagcag gacgcactac tcaccagggc
     5461 ggtgaatata caacgattcg aatatgaatc tacggcgctg acaaagcgca ataaccaaag
     5521 tgaactttgg ggtgtggtga agggttcatg gacgggaata tgtcgcacgt aaagcggcga
     5581 ggcctgcggg actattgccg aattgaagta ggccgaaaca ggtcaaaatg ggtctcccac
     5641 ctaccacacc accaaagttc atcaggaggt ctatatgaca cgcagaactc agttcaaagg
     5701 caattcacgt tctcgtcgtc gtgagcgttt aaaggcaaag gcattagcta acggcgtgct
     5761 gacccgcgaa gaagcaataa gttcagaagt attacaccgc cctactctaa gcagagcaca
     5821 gattcaggct aaaggtactc acgaaacgcc tgagcgcata gaagacgcta agccaattaa
     5881 gttcatggca caggacgtga tctggcaaca gaaagaatac agacgcaatc tggagcgagc
     5941 ggccattgtg tacgcgaatg agtttggaca taagcaacca gaaactggtg tatgtcttcc
     6001 aaacgtagct ctttacgcgg caggctacag gaaatccaaa cagctgacag caagatgact
     6061 tgtgttggtc gccagaaaat gaaatcaggc agcaaaccac ttatttgaga ggaattaata
     6121 tgtcatcaat ccgcttaaca acgagaataa aagaagtaat agcacgcaac gctttaatta
     6181 agtccggggt gttcactgaa cttgaagaag taacaaagct aaagaaccag cttgcacttg
     6241 atgccagagt tattgcgttt ggcgggaaga agaaaactga ggaagtggat cagttatcat
     6301 ccaggttaat ggctataagt aaagaactta aaaaactggg atgttcattt tactcatgcg
     6361 atgttctttc tacttcaatt tatctggccg tatctggcag aagtgttggc tggtattcat
     6421 acggaaaaga cagcaacggc gaagatatat ggctccctac tccgaccaga gataaatgca
     6481 tgtttgacgc agggcacgaa ataacaaaaa ggtttgatga aatctgcgca ttgcaacaaa
     6541 aacttgaagc caggaaaaag gatatcgaat caaatgtatg ggctgcttta aactcagtca
     6601 caacagttaa gcgacttatt gaagtttggc ctgaaagcaa agaattgcta ccaaaagaag
     6661 cagataaagc aagtacagca cttcctgctt tacgggtaga agatttgaat aagatgattg
     6721 gacttccttc cgaggccgca tagtcggcct ttatttttag cataaacaac agaataaaca
     6781 ctgcactgtg tattcattcc aaggagtgaa tacacggagc aatttaaagc gtaaataagg
     6841 agcacaccat gcaatatgcc attgcagggt ggcctgttgc tggctgccct tccgaatctt
     6901 tacttgaacg aatcacccgt aaattacgtg acggatggaa acgactcatc gacgtactta
     6961 atcagccagg agttcccaaa aatggataaa acacttatgg ctatccagac taaattcact
     7021 atcgccactt ttattggcga tgaaaagatg tttcgtgaag ccgtcgaagc ctataaaaaa
     7081 tggaggtcaa aatgattccg gtagagctgg cgaaaactcc agagttaagt cgattaaaaa
     7141 gagaatatca cattgctgag gctcgttact ggcgtaaagc gggagataaa tcaaagaaac
     7201 aactttgttt atggcaggca caaagagagc gcatgaatga gcgcgagttt ctttcctccc
     7261 catccgaatt accattctga ggcaaattat gggaactgcg acattaatac tcggtgaatc
     7321 tggtaccgga aaatcaacca gtttaagaaa cttaccacca gaaaaaacat taatcataca
     7381 gtgcataaac aaaccgctac cattcccatc aagtgattgg gttaaaataa gcaaggaaaa
     7441 caataacggg aacatataca gaacagataa tactgaaaat attgtaaggg cactaattaa
     7501 tgctccgcat gaaattgtag ttattgatga ttatcaggct gtgatggtta acgaactaat
     7561 gggtaaatgc aatgagagag gatatgacaa atttacctcg atagggaaaa acgcatggga
     7621 tgtgttcagg gctgctggtg aaggggcaga cactcggaga gtttatattc tggcacacac
     7681 gcaaactgat gattttggct atacacgaat gaaaaccgtc ggaaaactag tcgattcgac
     7741 acttgttcct gaaggttatt tcactatcgt gttaagaact cacgtacaga acggaaaata
     7801 ctcattttcc acccagacaa acgggagtga ttgttgtaaa agtccggttg ggatgtttga
     7861 tagtcagttt attgataatg atttgtacga agtagataaa aaaatatgct cttactatgg
     7921 gtttgaaaaa aatgagacgt cttgaggtat ccggcgagag atttggcaat ttagttgtta
     7981 ttggtgatgc aggaatgcgt ggtactcaaa ggttggtaaa ctgtgtgtgt gactgcggag
     8041 tagtttgtgt taaacagctt ggcaacctcc gttcagggca cacaagaagt tgtggttgct
     8101 cacgctcatc tacaacaaaa gcaagaaaaa acaaacatgg catgtatgga acgccaacat
     8161 acaagtcatg gtcttcaatg ttaacaagat gcttcaatga aagaaatagt aaatacaaac
     8221 actatggagg aagaggaata actgtttgtg aacggtggct ggactttaaa ttatttttcg
     8281 ctgatatggg aaaaagacca gacggaacta cattgggaag aattgataac aatggtaatt
     8341 acgaaccatc aaattgcgaa tggcagaaaa gtatcgagca agcaagaaat aagagtaata
     8401 ccgtattatt taaattcaat ggagtgctgg caacaatttc agagcactgc gaaaaaactg
     8461 gattaaaccc aagcacggta agaagcagga tttacacata tcattggtct gtagacaagg
     8521 ccttatcaaa aggataaaag catgagcaac gtgattttta cttataacga agaagcagca
     8581 ctgaccgcag ggcaaggtgg ttttattaac gaaactggcg ctcatatcat taccattact
     8641 gaagcagaac taaagcaatc agagaaagga gccaaattta ttgagttttc tggcgaatcc
     8701 gacgacggac gtaaaatcca atatcttagc gtttgtgttc agaaaaatga cggaacggaa
     8761 aacaagtttg gtgcaaacgt cgttcatgcc atgatggggt gtgttgggat taagcagtta
     8821 acgcaacata tggtttccgc cagtaaattt gttgctcctg aatttcatgg aaagaaaatc
     8881 gggttagtgc tccagaaagt attaaccaca aacaaaaaga ctggcgcaga cagctaccag
     8941 atggaaatac gcatcccgtt tattgcacaa acaggtcaaa cccttaaaga aaagtcggaa
     9001 ggcaagcaac cagaaactat cgccaacatg gttgccagcc tcaaagataa agacaatcgc
     9061 gctaaaaacg taagccagaa tcatgcagat gattatggtt acagccagaa cgattaccct
     9121 cctttctgat tactgaaaat aaggctccca ttatgccagc gcctctgtat ggtgcggatg
     9181 acccgcgcaa ctgctccggt agctccaagg cggaggtgct ggaaaatatc aaaaacaatt
     9241 tcgacgcgtt tcttgctctg acaccagaaa caaaagcaga acggatgtac cgacgcgata
     9301 tacaactcgc gctaaaacag gagaaggacc gaacaaacga aacagcaatg agaccgttgc
     9361 gaaaagcgac aatagacaaa ttccctgaat atatcgaccc gcgcctgcgt aattaccgct
     9421 cacgctatgg cgctatcagt aatgactgag gaatttacca tgagaggact tgcatacaat
     9481 cccggcattc ttccggcaga aatgattatt cgccaacgcg taaagccaat gccatcgaga
     9541 gaggaattgc ttaagcgaaa tagtttcggt tctgttaatg acaacaaata tctgaatgcg
     9601 atgtggcgga gtgggaagaa atgaaacaaa tgtcactaat tgagatggat ggatttctga
     9661 aaggtaaatg catcccacga gatttaaagg ttaacgaaac aaacgctgaa tatctggtgc
     9721 gtaaattcga tgaagtacgt gctgaggctc gcaacgaggg tattaactat accgcaagcc
     9781 gtcttgctgc tgcgttcaat cacggattta tcaataagtc tttgcgtgaa gttttcgacg
     9841 ttacacgcat gattttgtca gcgaaagaag agttggctaa tgaacagcat ccgattgatg
     9901 gcctgtctgg tggatatgcg gagaaatccc tagaagaatg ggcggaacag attcgcaaag
     9961 gaggcaacca gtgagcaaga ttgactatca ggcactgcgt gaggcggcag agcaggcaac
    10021 taaagggagc tacatcgtag ggcatacatc tgttaatcgg catggcaatt taacaggagt
    10081 ttttgtttgt caaaaatgga aaggagaacc cggtggtgtg attgcggaat gtcatgttaa
    10141 ctgcctggtt gaaacagatg ctcaggctta tgcaaacgct gaatttattg ctgcttttaa
    10201 tccaaatgtt gcgctggcac tactggatga acgggaaaga aacctgcaat acatcaaaag
    10261 ccgcgatcag gagaacgagg aaattgcgct aacggtaggg aagttgcgtg ttgagctgga
    10321 agcagcagag aacaacctta ttgatagtga atgccatgtt gctgaactgg aagaagcgct
    10381 acgcgataag caggcgttac ttgaagcctc agaaaagcgc atagcagaac tggaggcaca
    10441 gacagttacc gttaaagagg ttggagatgc ttgatagttt tgcgctcgtt gcactggttg
    10501 gcattgtagc gttgatttgg gtgacatccg ggaatcggag gcataagtga acgacaaaga
    10561 actgattaaa gaaatcaaag agcgtatcag cagtttggat gtgcgagaca atgttgaacg
    10621 ccgtgcttat gaaattgctc tggcctcgct gacagcagag ccgataggtg ctttccacat
    10681 tgcagaacag caggtcgatg gcacaagtga ttacatcaag gatggggatt ggcctattga
    10741 taatgggata attgaagtat acgccgcccc tccagcgcca atagtaccgg aagaaaaacc
    10801 aatgcctaat cctcttagca tgtacgcggt tgatgctgtt gccgctattg cagaggtgag
    10861 aggctggaac gcctgtcgtg ccgctatgct tcagagttaa gataattgca ttaaggatgc
    10921 ctggataagc tgtagtgagc gaatgccaga aatgggagag cgacaatgct atgtgttagc
    10981 agctgacttt aaaaacaact acccaccaaa catccccaac actcaggtcg gcgtatatgg
    11041 cgactggttt aatgatggca atcccacttg ggatgacggt gatggcgaag acctgtatct
    11101 caaagaggta acccactgga tgcagctacc agaaccgccg caggaggtta accgtggcta
    11161 acctgcaact tgccgtaaaa ggtgaataac aatcctcgca ttcgcggaga tttcttttat
    11221 ctgaactcgc tacggcgggt tcaccgtcaa gctttcttca atgcaaaagc caatgtgagg
    11281 acgctatgaa tactcaaatt tctgtcgaaa cactttcacc aatcacctac aaacaaacca
    11341 acgtcatcac cagcgaacta ttggcgcagt tgtacggaac ggatgttgtg cgcatccggc
    11401 aaaaccataa tgaaaacaag tcgcgctttg ttgagggggt acacttctac aaggtcgttg
    11461 gtgacgagct gcgcgcgcta aaacacaaag tagctttaaa ctactttgtg aaaattgccc
    11521 caaatgcacg ccacctcatc ctctggacag agcgcggagc cgcccgtcac gcgaagatgc
    11581 tcgaaacgga tcaggcatgg gacgtgttcg aaaaactgga ggacggctac ttcaatcagc
    11641 gcgaaaaggt tgccaaccat cctcaaccag taccgcaacc agaaagtagc ctagaccgtg
    11701 cgcgagccat ggaaatagct ttgaacgtgg cggatcgact ggtgaatatg ttcccgaatc
    11761 tgtcctcaga atcaaagcag gcagcagtag ctggagtgtt caacccgatt gttggtcgcg
    11821 atgttgtacc tctgccagct atctcagagc actactactc tgctacggaa gtcggccagc
    11881 gctacggtat ttcagccaac aaggtaggac gcatagccaa cacctacatg ctcaaaacag
    11941 aaaagtacgg gaagtggttc atcgacaaat cggcgcacaa tgacaagcag gttgaaacat
    12001 ttcggtacaa cgaggctgga gtgcacaaaa tagaggaact catcgaagga gggcgaaagg
    12061 ctgcatgatt ttgacaagat agttttcccc aaatgtgggg aaaagcccga atggcgcggc
    12121 ttacagcaag ataaggccta catgatttga caacaccgca ttaacggggc tatattccgc
    12181 ttcatggtgc tcaaaacacc tttgcgatag cggaaaccgc cccgtcagtt atgcggattt
    12241 ttttatgtct acgacattga gaattcaatg gtcgggcgtg cggctaataa aacacccgca
    12301 agggaaatat gcccgccgtc tatcgccggt tttgagcgcc cgaccaccct ctcaaaaggg
    12361 gtaaattcaa aatatgcgat aggacataaa accatggcta ctcaaatttc tgttgaatcc
    12421 ctttccccaa ttacctataa ccacatcccc gtcatcacta ccgaactact ggcacgactt
    12481 tacggcactg agaccaacaa cattaaagtg aactatacgc gcaatgccgg gcgttttgtt
    12541 tgtggtaagc actactttaa actggagggt ggtgaattgc gggaatttaa gaacaaggtt
    12601 actcacagta acttagttgc accgcgagca aagcacctca tcctctggac agaacgaggc
    12661 gcagcccgcc acgcaaaaat gctcgaaacc gatcaggcgt gggaagtgtt cgaaaaactg
    12721 gaagactgct atttcagccc gggcaaagcg gaacagcacc cgcagatcca gcagcaattc
    12781 acagacgaag aaatcatcct cctttgctat atgcaggtac agatggagaa tgcgctggac
    12841 atctgcaaac gtctgtaccc gatattgaag gaactgaact catcatacac gagcaagctg
    12901 tatgacatcg cgtttgaaac tttttacacg gtgacgaaaa acagagatgt actgctaagg
    12961 gaggcgacac gacttgacca agcaagcgcc cttttcgaac gggcaaaacc aatgctgaaa
    13021 agccttcggg cgagacaatt cgaattttaa tcatcaaagg agcttcggct ccttttttgt
    13081 tggagaaaat aaaccaatac tcgctccctt gcgagtaatt gcggagactt tgcgatgtac
    13141 ttgacacttc aggagtggaa cgcacgccag cgacgcccaa ggagccttga aacagttcgt
    13201 cgatgggtac gcgagtgcag gatattccct cctccggtta aggatggaag agagtatttg
    13261 ttccacgaat cagcggtaaa ggttgactta aatcgaccag taacaggtag ccttttgaag
    13321 aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta
    13381 tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag agtttggatt
    13441 aggccgagac aggcgaatcg caatcactga agctatacag gccaacattg agttattttc
    13501 aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattctg ttacgttaca
    13561 ttcatggctt gatcgctacg aaaaaatcct cgccagcaga ggaatcaagc agaagacact
    13621 cataaattac atgagcaaaa ttaaagcaat aaggaggggg ctgcctgatg ctccacttga
    13681 agacatcacc acaaaagaaa ttgcggcaat gctcaatgga tacatagacg agggcaaggc
    13741 ggcatcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg caatagctga
    13801 aggccatata acaacaaacc cggtcgctgc cactcgcgca gcaaaatcag aggtaaggag
    13861 atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat catcaccatg
    13921 ttggctcaga cttgcaatgg aactggctgt tgttaccggg caacgagttg gtgatttatg
    13981 cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa gcaaaacagg
    14041 cgtaaaaatt gccatcccta caacattgca tgttgatgct ctcgggatat caatgaagga
    14101 aacacttgat aaatgcaaag agattcttgg cggagaaacc ataattgcat ctactcgtcg
    14161 tgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa aagcatcagg
    14221 tctttccttc gaaggggatc cacctacctt tcacgagttg cgcagtttgt ctgcaagact
    14281 ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcaca agtcggacac
    14341 catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg aaatcaaata
    14401 atgattttat tttgactgat aatgacctgt tcgttgcaac aaattgataa gcaatgcttt
    14461 tttataatgc caacttagta taaaaaagca ggcttcaacg gattcatttt tctatttcat
    14521 agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc attggctttt
    14581 ggcgtgacgc tgaccgcctg tagctcaaca ccacccgatc aacgtccttc tgatcaaact
    14641 gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgctgaa tttcgatgcg
    14701 caacactatt ttgcatccct gacaccaggt gctgcagcgt ggaatccgtc cccgataacc
    14761 ctgcctgcgc aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt aacgcatacc
    14821 acgattcagg cggcggtaga tgcggcaatt atcaagcgca ccaacaagcg ccagtatatt
    14881 gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc ctgctgctcc aggtggaatc
    14941 actctgtacg gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc ccttgatggg
    15001 ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata tatgccaggt
    15061 aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag tatcggtgtt
    15121 ctgtgttctg cggtcttctg gtcacaaaac aatggactgc aactgcaaaa tctgaccatc
    15181 gaaaacacgc tgggcgatag cgtagatgca ggtaaccatc cggcggtagc actgcgtact
    15241 gacggtgacc aggtacagat taacaacgtt aacattctcg gtcgtcagaa caccttcttt
    15301 gtcaccaaca gcggtgtgca gaaccgtctg gaaaccaacc gtcagccgcg tacgctggtg
    15361 accaacagct atattgaagg ggatgtggat atcgtttctg gtcgcggcgc agtggtgttc
    15421 gataacaccg aattccgcgt ggtgaactca cgtacccagc aagaagcata tgtgtttgca
    15481 ccggctacgc tgtctaacat ctattacggt ttcctcgccg taaacagccg tttcaatgct
    15541 tccggtgatg gcgtggcgca actgggccgc tcgctggatg ttgatgctaa taccaacggt
    15601 caggtggtga tccgtgatag cgccatcaac gaaggtttta acacggctaa accgtgggcc
    15661 gatgcggtga tctctaatcg tccgtttgcg ggtaataccg gcagcgtaga tgataacgac
    15721 gaaatacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata caataaccgc
    15781 ggcgtgggca gcaaagtggt tgcagaggcg aagaagtaag agctattaac tatttgccgg
    15841 atgcggcgta aacgccttat ccggcctacg gttcgatgcg atttgtaggt cggataagat
    15901 gcgccagcat cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt
    15961 tacatctgac ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct
    16021 tcagccgtca ggctttccat gtaaagcgta atttccgtta ccggcgcatc ttcatggatc
    16081 acataacctt taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa
    16141 atgtaatccc ccacttcaaa ggttggcact tccgccatct gcagcggtaa catcccccag
    16201 ttgatgacgt tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg
    16261 ccaatcacac gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc
    16321 gcatagacca tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc
    16381 tgcttaatgc gcgcaaacac ctctgtcagc tcgctgacat tccccgccag accaatcgga
    16441 tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt ggtgacttcg
    16501 tcgaggatct tcgagcacac tttcaggacg atattgtcag tcagcgcacc caattccggc
    16561 cagtctttaa tgttcggtcc gtaaatcagc ggttgctgag ttgccccttt cacaaagccc
    16621 tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac gttgtcccag
    16681 caatcaagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa
    16741 cgagcgtcca tcaacgccac cgctgacatc tgcccattgg ctggcttaga gccttcgcgg
    16801 ttcgggaagt tacgcgtggt gtggcgaata ctcaaaccgt tgttgattgg cgtatcgccc
    16861 gcgccaaagc atgggccaca gaatgcggtt ctgatgattg cgcctgcgcc aatcaaatct
    16921 gctaccacac cttttttggc gagatccata aacaccggct gtgatgacgg gtaaaccgcc
    16981 agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc cgcgatgacg
    17041 ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt caggcgacca
    17101 ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg ttcggactca
    17161 atttcaatct cacgcagaat gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg
    17221 ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg
    17281 cagccatcgt agtacgccat cggttgaggg ttaagctggc agtaatcctg gccgcgaccg
    17341 tgcagcgcca gccagttatg cacttcttca tcagtttgcc agacggagct taaacaggtg
    17401 gtttccgtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc gctaacgccc
    17461 ggtccaacga actccatgac tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg
    17521 atagccagcg ccacatcctg tggccccaca tacggcgcag gttttccggt cagatgcacc
    17581 gcaaccacgc ccggatagtc gatatcccag gtgtcattaa gcagctgttt taccaactca
    17641 ccgccaccct caccgactgc cattgtccct aatgcaccgt aacgggtgtg gctgtctgac
    17701 ccgaggatca ttttgccccc gcctgccatc atctcacgca tatattgatg gatgaccgca
    17761 atatgcggag gcacaaaaat accgccataa cgctgagctg ccgataaacc aaaaacatgg
    17821 tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt ggtcagcaca
    17881 tacggcagcg ggaaacgttc cattccggac gctttagcgg tctgtacaat accaacaaag
    17941 gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat atttccggac
    18001 gtattatgag aagagagaat agaccaggca atagtgcctt tttttgcttc ttctttttta
    18061 atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag aaacacgcct
    18121 ttttcagata acttgatcat caaatgctcc agatatctgc gcaccgtgaa gtgcgcagaa
    18181 agataataaa tggatatact ctaaataatt cgagttgcag gaaggcgaca agtgagtgaa
    18241 tccccaagag cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca
    18301 acttgaagta tgacgagtat atttacagca tgttccagcc gatcagcata ttccaccacc
    18361 acacgccgag ggtgatatgc accaggaagg ttaatatcgt cagtaccgca ccgaccaacc
    18421 atcaggattt aatatcgttg taacccacgc caaagatgac cggacctgcc gcgccgccat
    18481 agtgagtaac catgccgcca taggagttgg agaacaacag tgccagcgcg gttaacatta
    18541 acggtgcgcc ggagacgttc gccagcatgg caaatacagg taacatagca acgatatagg
    18601 cactaccgga agcgaagaaa taacgcacga tgatgctgag gaaaataata acgaagaaag
    18661 caacgttacc gtgaccatca aatgccaggt tatttttaaa gacttcagct aaccattcga
    18721 aaaatttaac tttcgataat aaggagctta agccgataat accgccgtac cagattaagg
    18781 tattccagcc gcctttattt ttaaccacgt cttcccaggt aacgataccc agcagcagca
    18841 tggtcgccat aacaacgatt gccacagtgg attcatcaac ccccagagac ttactgaaaa
    18901 tccagcccag tagcgccagc acaaagacac cgagcagcat tttttcgcgg attttcatcg
    18961 gtcccagttc tgcaaggccc gctttagcga tggttttgtt atccaccttt ttaatttctg
    19021 gtggatacat ggtgtaaatt accagcgggg tgaccagcag cataatgatg cccggcaatc
    19081 cggcggccag cgcccatcca ccccagctaa tttgcaggtg cagaatgtcg ttgatcattt
    19141 tcagcgccag aatgttcccc tccattgcgg taaagaacat atagctggtg gttttggtga
    19201 ccatgtaaat ggacatcatc aggtaatgtc cgacacgacg cggacttttt tccggttcgg
    19261 accccaaagc caccgccacg ctgttgatga tcggtaacac aatgccgccc gcacgcgcgg
    19321 tgttagacgg tgttgccgga gccagtacca gatcgaggaa taccgtaacg taacccagac
    19381 ccagcgtggt gttaccgatt ttaccaatca gcagataggc aatacgttta cctaaaccgg
    19441 tggtcacaaa tgcggcgctt aaggtaaacg ccgagaacac cagccaggta gtacctgaag
    19501 agtaaccgct taatacggcg gtggtcttaa acgcaccgtc ggataagtta ccgaccacca
    19561 ccattgaggc agcaacggca attaacagta cgacaggttc cgggaaaggc ttgataacca
    19621 gacccacaat ggccgccagg taaataccaa aaagcaccca cgccagttcg cttaatcctg
    19681 ccggagctgg cataaaacca ataatacatg ggatcgctaa tatcagaatt agcttccata
    19741 acgatttctt attcatttaa aatcctgttc tttctgtatg actcatacag aagtccttta
    19801 ttgattgtcc ttatcctgac aacgaatttt ttcagggaag ataaacttca ccggaaaata
    19861 tttttctggt tgtccgaata acagatgcgc gtaacgtcgt ggcatcctga ccttcattac
    19921 ttaaatgaac gtcgagcgca ccactggggt gttcaatatt gatattgcca tatcctacag
    19981 aagggacgat ttgtcgggtg acggtgcctt ccaatgcaca actactggaa atagcaatag
    20041 caccggttat cgccagcgcg cgatggcaag aatgcggcat aaaataacgc acattaattg
    20101 ccccgccttt ctgcgctgga gaaataagca caggtttagg gataaccata ttactgacat
    20161 cacctaagcc cattgcttta cccgcttgta gccggataga ttcaatgcgg gctaataatg
    20221 ctttgtcggc atccagttcc gccggtaatt cataacctgt ttttcccaga tattcagccg
    20281 gaataatgac gactggcatc gccatatcga tacaggtcac cgggacatcg tcaaaataat
    20341 caatctgatt atcagtcggg aaaacttttc cggttttcgt tccagcggca ttcaggaaag
    20401 tgagcgcaac cggtgcggca gtacccggta cgccgtcaat tctggcgcta ccctcgtact
    20461 cgacaacacc atttggcgtt tgcacatcag cttcgatgaa cgtacccgta ttgacgttgc
    20521 ggatacgtac acgggtaact ggcgaagtcg ctgcaatcaa accattttca atggcgaatg
    20581 cccctacgcc agacagcata ttgccgcagt taggcgtggt atcgacacgt tgctcatgga
    20641 cgataacttg agcaaacaga taatcgacat cagcacgcgg atcgctggaa cggctaataa
    20701 tggcgacttt actggtcagt ggattaccgc cgccaatacc gtcaatttcc agatcgttac
    20761 cggaacccat aattgccatc aatattttat cgcgctgcgt ttgatcttcg ggtaaatgtt
    20821 ccgctaacag gaacgcgccc ctcgaggttc cacctcgcat catcacgcag ggtatttttt
    20881 tcataataat gccctcaatt taaaaacata acttattgcg atatggttac attaaggaca
    20941 aagcatctct aatgacttta gctcaacgat taataacatt ttttcattaa tttaagttgg
    21001 ctaaatgcag ttaaaatttt taacgaccag ccacc
//
LOCUS       DADLSE010000061        20991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLSE010000061 DADLSE010000000
VERSION     DADLSE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.061"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..333)
                     /locus_tag="JN692_002255"
     CDS             complement(<1..333)
                     /locus_tag="JN692_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7264560.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRV"
     gene            complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN692_002256"
     CDS             complement(511..1650)
                     /gene="actP"
                     /locus_tag="JN692_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7264561.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALSLGLGLM
                     FGTAGLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYK
                     DAAGHLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLY
                     ANVFKKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPI
                     ILLSMYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSI
                     SVAFLGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1647..1961)
                     /locus_tag="JN692_002257"
     CDS             complement(1647..1961)
                     /locus_tag="JN692_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7264562.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN692_002258"
     CDS             complement(2161..4119)
                     /gene="acs"
                     /locus_tag="JN692_002258"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7264563.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4512..5948
                     /gene="nrfA"
                     /locus_tag="JN692_002259"
     CDS             4512..5948
                     /gene="nrfA"
                     /locus_tag="JN692_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7264564.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5993..6559
                     /gene="nrfB"
                     /locus_tag="JN692_002260"
     CDS             5993..6559
                     /gene="nrfB"
                     /locus_tag="JN692_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7264565.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6556..7227
                     /gene="nrfC"
                     /locus_tag="JN692_002261"
     CDS             6556..7227
                     /gene="nrfC"
                     /locus_tag="JN692_002261"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7264566.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7224..8180
                     /gene="nrfD"
                     /locus_tag="JN692_002262"
     CDS             7224..8180
                     /gene="nrfD"
                     /locus_tag="JN692_002262"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7264567.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8200..9918
                     /locus_tag="JN692_002263"
     CDS             8200..9918
                     /locus_tag="JN692_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7264568.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9911..10294
                     /gene="nrfF"
                     /locus_tag="JN692_002264"
     CDS             9911..10294
                     /gene="nrfF"
                     /locus_tag="JN692_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7264569.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10291..10887
                     /gene="nrfG"
                     /locus_tag="JN692_002265"
     CDS             10291..10887
                     /gene="nrfG"
                     /locus_tag="JN692_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7264570.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11229..12542
                     /gene="gltP"
                     /locus_tag="JN692_002266"
     CDS             11229..12542
                     /gene="gltP"
                     /locus_tag="JN692_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7264571.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12620..13309)
                     /locus_tag="JN692_002267"
     CDS             complement(12620..13309)
                     /locus_tag="JN692_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7264572.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN692_002268"
                     /pseudo
     CDS             complement(13403..15550)
                     /gene="fdhF"
                     /locus_tag="JN692_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN692_002269"
     CDS             complement(15748..17214)
                     /gene="mdtP"
                     /locus_tag="JN692_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7264573.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTHLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN692_002270"
     CDS             complement(17211..19262)
                     /gene="mdtO"
                     /locus_tag="JN692_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HAY7264574.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN692_002271"
     CDS             complement(19262..20284)
                     /gene="mdtN"
                     /locus_tag="JN692_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7264575.1"
                     /translation="MESTPKKAPRNKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGRIIS
                     LKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEG
                     KVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIIVDKPDPEMFRIGASAV
                     ANLEPQ"
     gene            complement(20303..20578)
                     /locus_tag="JN692_002272"
     CDS             complement(20303..20578)
                     /locus_tag="JN692_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264576.1"
                     /translation="MPTVLSRMAMHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20787..>20991)
                     /locus_tag="JN692_002273"
     CDS             complement(20787..>20991)
                     /locus_tag="JN692_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HAY7264577.1"
                     /translation="RKTLQPQVDASFYISREDLHAVLTGQAKMADLVKAKRAKIIGNG
                     AKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         4719 a         5476 c         5698 g         5098 t
ORIGIN      
        1 aactcttctc gtaattctat cgtcacattt attatttgca tataacgcta tatctataaa
       61 atatttatgt attttcctgt ccgtttcttt cgtgattttt cctgatggta aactatcata
      121 aattcttttc attttctgaa tcaagcctat cactttttca tgatcatcta ttttgaaaag
      181 agagagggct ttattgtcaa ttaacgtgtt tttatgatgt ttgctgtaat tatctgattt
      241 tgttttcttt gtatgtgatg aacttgtgat agagtggtta tcgttaatct ggcaaagagg
      301 gaaaaacata ttatttaaac cattatagcg cataaaatat ccttataatt aacaagaaag
      361 aaaaggcata ttctctgcat tatcattttc tacgactgtc aaaaatcgct cattttttaa
      421 tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat gttggagagg aagagtattc
      481 cccggtcagg cgaccgggga aggggagaga ttaatgtgcg cggccttgct caacgccaaa
      541 gccggtctgg gagcggataa actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc
      601 tgagttatcg gttgccgaga agaaccagat gccgaggaat gccacgctga tagagaacag
      661 cgccgggtat tcatacggga agatggcttt ttcgtgaccg aggatctgta cccaaatagt
      721 cgggccgagg atcatcagca ccactgcggt aatcagcccc agccagccgc ccagcatcgc
      781 gccacgggta gtcagtttcg accagtacat ggaaagcaga atgatcggga agttacagct
      841 cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg ttctgattct caaacagcac
      901 gccgaggata atcgcaatca cgccgaggat cagtacggtg atttttgata cccgcagctc
      961 ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac aagtcatgcg aaaccgccga
     1021 tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata gtggcgaaag caaccgctga
     1081 aataaaaccg aggaataggt tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt
     1141 gttaccacca atcagatggc ccgccgcgtc tttatattcc ggattcgcac caacaagcat
     1201 gatcgcgccg aagccgataa taaaggtcag aatatagaag tagcccataa atccggtggc
     1261 gtagaacacg ctcttacgtg cttcgcgggc atcgctgact gtaaagaagc gcatcagaat
     1321 gtgcggcaag cccgccgtac caaacatcag tcccagcccc agagagagcg cggagatccc
     1381 caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt
     1441 gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta
     1501 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1561 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1621 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta
     1681 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag
     1741 atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc
     1801 ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1861 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     1921 tgcgcattgt cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta
     1981 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2041 ttgccgaatg taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg
     2101 acgctgtcgc gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg
     2161 ttacgatggc atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc
     2221 ggcaagcgtc gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat
     2281 acggcgcata attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc
     2341 tggcgtcgcc agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc
     2401 tggtgacggt tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat
     2461 attgtgcgga atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga
     2521 ctctatctct gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc
     2581 ggtgatccag taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt
     2641 tttgaaggtg gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc
     2701 ctgaccaggc caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt
     2761 accttcgtta tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc
     2821 tttcagctcg gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca
     2881 ccaggtatcg accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc
     2941 ttccgggtta attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc
     3001 ttcaatcgct ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag
     3061 aatattgacc tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg
     3121 tacgccttca aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact
     3181 gtgtccggta acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc
     3241 aaagacatat ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac
     3301 gccttttggt tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt
     3361 catctcttcc gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc
     3421 gcgcccttcc tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac
     3481 gctggtgacg ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg
     3541 cccggcgcgc acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg
     3601 cccggcaacg gcttccggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg
     3661 ggcgcaggcc agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac
     3721 atcacctttt ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg
     3781 cagctcttta tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc
     3841 ggtgcgatcg ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt
     3901 gccgtcctcg taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt
     3961 ctggtacggt ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac
     4021 gttaatagat tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc
     4081 gatgttggca ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga
     4141 tgttaataat atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt
     4201 tgggcgacag atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag
     4261 gtcaccaaca aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa
     4321 agaggaagat actgactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag
     4381 acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca tgacagtgta
     4441 ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg
     4501 atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc
     4561 cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa
     4621 ctgtggaagc gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga
     4681 aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca cggctggtga
     4741 tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg
     4801 ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc
     4861 cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag
     4921 acggcgaaga tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca
     4981 acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg
     5041 agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg
     5101 agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt
     5161 acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa
     5221 atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa
     5281 cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta
     5341 aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac
     5401 tctacaccga ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact
     5461 gccataccca ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta
     5521 acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg
     5581 cgctggatgc aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg
     5641 cccagtggcg ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag
     5701 aaggtctacg gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg
     5761 cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca
     5821 aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg
     5881 acttcatcaa aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa
     5941 gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt
     6001 attacgttcg ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg
     6061 aattaccgct acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg
     6121 taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa
     6181 acatgcttcc gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg
     6241 ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat
     6301 gtacaaggtc ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca
     6361 aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc
     6421 cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg
     6481 cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt
     6541 gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg
     6601 gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg
     6661 cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct
     6721 tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag
     6781 ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc
     6841 gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc
     6901 gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt
     6961 ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt
     7021 cgtaagacca atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag
     7081 gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag
     7141 aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg
     7201 tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg
     7261 tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga
     7321 cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc
     7381 tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct
     7441 tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg
     7501 tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg
     7561 cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga
     7621 tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa acgctgatgc
     7681 tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt
     7741 atccgttcct caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg
     7801 gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg
     7861 aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg
     7921 tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg
     7981 cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga
     8041 ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg
     8101 cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg
     8161 gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa
     8221 gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc
     8281 tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg
     8341 gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc
     8401 gatttctcgg tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa
     8461 ctggcggcgg tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc
     8521 gcctggagcg cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg
     8581 acgctagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc
     8641 gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg
     8701 caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg
     8761 gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc
     8821 cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc
     8881 ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg
     8941 gaaaacgcct ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg
     9001 acacgccagc gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg
     9061 ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc
     9121 gcactggata acgtccgcgc tgtgccgttg ttcagcctgt ttgcactgat tagccttgcg
     9181 tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg
     9241 ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc
     9301 gtgctggtgg gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc
     9361 gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg
     9421 attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat
     9481 gctggcgtgc tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc
     9541 agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag
     9601 cgactcgatc tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt
     9661 gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt
     9721 cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg
     9781 ggtgaaaaaa ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc
     9841 tggatctggg ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg
     9901 aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg
     9961 cgctcaggtt gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa
    10021 tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc
    10081 gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga
    10141 agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc
    10201 gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc
    10261 actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg
    10321 ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca
    10381 aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc
    10441 agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca
    10501 caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc
    10561 aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat
    10621 gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc
    10681 gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg
    10741 gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg
    10801 atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca
    10861 aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg
    10921 cataaaaaat aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga
    10981 ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg
    11041 cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc
    11101 gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg
    11161 ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag
    11221 ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc
    11281 tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg
    11341 tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc
    11401 cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg
    11461 ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt
    11521 tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg
    11581 cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg
    11641 gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag
    11701 gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc
    11761 ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta
    11821 aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga
    11881 cggtggctaa ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc
    11941 atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt
    12001 taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg tactccactg
    12061 ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg
    12121 cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc
    12181 tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct
    12241 ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg
    12301 ctggcgtgtc gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag
    12361 gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg
    12421 tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca
    12481 aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat
    12541 aattgaagat tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc
    12601 gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat
    12661 caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga
    12721 taaaaccttc ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc
    12781 gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc
    12841 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct
    12901 ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg
    12961 cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
    13021 ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt
    13081 tgatctgcgc caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc
    13141 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
    13201 cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg
    13261 cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac
    13321 gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa
    13381 ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact
    13441 tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc
    13501 gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca
    13561 gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac
    13621 gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg
    13681 cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt
    13741 catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac
    13801 caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg
    13861 ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa
    13921 acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc
    13981 ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact
    14041 catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt
    14101 cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac
    14161 gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg
    14221 gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca
    14281 gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat
    14341 cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcaactcgc
    14401 tgatgcgata gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct
    14461 cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca
    14521 tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct
    14581 taccgaggtt accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct
    14641 ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca
    14701 tccgtgccgc ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg
    14761 gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga
    14821 acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga
    14881 tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc
    14941 gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca
    15001 cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa
    15061 tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg
    15121 atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg
    15181 caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct
    15241 ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca
    15301 gtgcctcatc tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg
    15361 ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct
    15421 tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc
    15481 cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga
    15541 cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt
    15601 atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg gcactattta
    15661 ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg
    15721 cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata
    15781 cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat
    15841 cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct
    15901 ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac
    15961 gtgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agatgcgtgc cgttgacggc
    16021 aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc
    16081 ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag
    16141 acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca ggtggatggc
    16201 atcaaggccg aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc
    16261 cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc
    16321 gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg
    16381 tttgatctcc ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt
    16441 ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg
    16501 cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt
    16561 caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc
    16621 ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt
    16681 ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc
    16741 ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc
    16801 gtccatcccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac
    16861 acgttggcgg ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa
    16921 atcggcctgc gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc
    16981 acttagcgtc cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg
    17041 cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg
    17101 tttgagctgt tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt
    17161 cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg
    17221 gctccttgtg cctgttctgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg
    17281 gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag
    17341 gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca
    17401 gcgtatttct ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc
    17461 agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga
    17521 ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc
    17581 agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga
    17641 atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta
    17701 cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc
    17761 cagacaaagg tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga
    17821 tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg
    17881 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt agcaatccat
    17941 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18001 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18061 cgcagcacca tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc
    18121 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa
    18181 caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg
    18241 accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt
    18301 tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt
    18361 tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg
    18421 ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag
    18481 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18541 caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt
    18601 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc
    18661 aggtggctaa tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca
    18721 cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca
    18781 acgatacacc acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag
    18841 gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    18901 cgcatcaaaa acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    18961 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19021 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19081 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19141 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19201 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19261 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19321 gtttatcgac tatgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac
    19381 gagacacttt tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19441 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19501 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19561 agtgacgagt gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19621 aggttttgag ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat
    19681 gtagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19741 cgccgctggc ggctgactgc gcctgtaaca atacggcatt aagatccgct tctgcggcgc
    19801 gctgcgccgt tcttgcacgg tcaacgtctt ctgctgagac aaaaccctct ttcagtaatg
    19861 gctcggtgcg gcgtaatgta tctgtggcct gtttcgcggc ggcacgggct ttttctaccg
    19921 tggcattaac cgagtcggca ccaaactgtt gggcgtcaac gctacgctgg gtgagcataa
    19981 tttgcttatc cagcgccgcg agggaggctt cggctttcgc caggttggct tcgtacgggc
    20041 gcgggtcgat gcggaacagc aaatcgccct gtttcaccgc ctggttgtcg gtgaccgcca
    20101 gttctacaat gcgaccgctg acttccggca ccacatcaat ggtatctgct gacgcgtaag
    20161 cgtcattagt tgatggcgca ctgtctacgc gccagataac gaaaacaagg gcaaccagcg
    20221 ccaacgcaac cactaacaga gcagggaatt tattgcgagg agcttttttc ggcgtacttt
    20281 ccatgagcgc gtctcaaata gattagaaaa atgccagcca gaacagcatg gcgtagagag
    20341 caaaaagggc ggtataaata attccgacaa atgccagatt gatatttgcc cgctgaataa
    20401 ctcgcctcgt gatgagcgtc aaaataaggc tggcaatggc gcagaaaaac cagctgggat
    20461 aataagcgcc gatcaccggg attgccggag ataatgagca tcccgaaacc atgaagacgg
    20521 gaattatcca ggctgttttt ttgaggtgca tcgccatgcg agagagaact gttggcatat
    20581 taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa tatcaatatc cattgaaggc
    20641 gtcagtatgg ctattttctc gatgaataaa aatagcttta tttagccacc gcattcataa
    20701 cttatatatt ttatttatta attggctgta tatattttta aaataaatgc ttttgggggc
    20761 ttcagcgccc ccaaaagcga gtgacattaa tttggggtta cgatattcac ccacaaatcg
    20821 aaattatcca gacaggcgat aatttcttcc agtttcgcgc cattgccaat aattttggct
    20881 ctcttcgctt tcaccagatc cgccattttg gcctgtccgg tcagcacggc gtgcagatct
    20941 tcacggctga tgtagaaaga ggcgtcgact tgcggttgca gtgttttacg g
//
LOCUS       DADLSE010000062        20851 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLSE010000062 DADLSE010000000
VERSION     DADLSE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.062"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..882
                     /locus_tag="JN692_002274"
     CDS             <1..882
                     /locus_tag="JN692_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264578.1"
                     /translation="RNYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFST
                     FLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVV
                     TQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGI
                     AAGLEIPMMLIAGYYMKRIDKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIF
                     LGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLA
                     IGMLGIAMICLLFIKDI"
     gene            993..1916
                     /locus_tag="JN692_002275"
     CDS             993..1916
                     /locus_tag="JN692_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7264579.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN692_002276"
     CDS             complement(1920..2738)
                     /gene="nlpA"
                     /locus_tag="JN692_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7264580.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAQEDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2960..3252
                     /locus_tag="JN692_002277"
                     /pseudo
     CDS             2960..3252
                     /locus_tag="JN692_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN692_002278"
     CDS             complement(3293..4483)
                     /gene="nepI"
                     /locus_tag="JN692_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7264581.1"
                     /translation="MSEFIAENCGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNIFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIGSFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4694..5146)
                     /locus_tag="JN692_002279"
     CDS             complement(4694..5146)
                     /locus_tag="JN692_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7264582.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5199..6533)
                     /locus_tag="JN692_002280"
     CDS             complement(5199..6533)
                     /locus_tag="JN692_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7264583.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6708..8460
                     /gene="adeD"
                     /locus_tag="JN692_002281"
                     /pseudo
     CDS             6708..8460
                     /gene="adeD"
                     /locus_tag="JN692_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
     gene            complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN692_002282"
     CDS             complement(8506..9897)
                     /gene="uhpT"
                     /locus_tag="JN692_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7264584.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN692_002283"
     CDS             complement(10035..11354)
                     /gene="uhpC"
                     /locus_tag="JN692_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7264585.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN692_002284"
     CDS             complement(11364..12866)
                     /gene="uhpB"
                     /locus_tag="JN692_002284"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7264586.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN692_002285"
     CDS             complement(12866..13456)
                     /gene="uhpA"
                     /locus_tag="JN692_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7264587.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13531..13821)
                     /locus_tag="JN692_002286"
     CDS             complement(13531..13821)
                     /locus_tag="JN692_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7264588.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFSRRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN692_002287"
     CDS             complement(13825..15513)
                     /gene="ilvB"
                     /locus_tag="JN692_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7264589.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN692_002288"
     CDS             complement(15619..15717)
                     /gene="ivbL"
                     /locus_tag="JN692_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7264590.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            16282..16371
                     /gene="tisB"
                     /locus_tag="JN692_002289"
     CDS             16282..16371
                     /gene="tisB"
                     /locus_tag="JN692_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7264591.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16651..17835
                     /gene="emrD"
                     /locus_tag="JN692_002290"
     CDS             16651..17835
                     /gene="emrD"
                     /locus_tag="JN692_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7264592.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17843..18340)
                     /locus_tag="JN692_002291"
     CDS             complement(17843..18340)
                     /locus_tag="JN692_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7264593.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(18337..18699)
                     /locus_tag="JN692_002292"
     CDS             complement(18337..18699)
                     /locus_tag="JN692_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7264594.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18689..19036)
                     /locus_tag="JN692_002293"
     CDS             complement(18689..19036)
                     /locus_tag="JN692_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7264595.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            19145..19558
                     /locus_tag="JN692_002294"
     CDS             19145..19558
                     /locus_tag="JN692_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264596.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19605..>20851)
                     /locus_tag="JN692_002295"
     CDS             complement(19605..>20851)
                     /locus_tag="JN692_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7264597.1"
                     /translation="MGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWDADYWFDGA
                     NYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQPVRAEEPFL
                     MVVSYDEPHHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQAMPSPVGDD
                     GLYHHPLYFACNDFVDDQIGRVINALTAEQRENTWVIYTSDHGEMMGAHKLISKGAAM
                     YDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGENILAVKEPR
                     GVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPNEMHNLIDD
                     IRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPRPQDGYSPV
                     VRDYDTGLPTQGVKVEEKKQKF"
BASE COUNT         5194 a         5610 c         5202 g         4845 t
ORIGIN      
        1 cgcaactact tcattctcct ctctacgggc gtacttctga gtagttttgc ttctaccgca
       61 aacccgcaaa tgttcgccct cgcccgtgaa cacgccgaca gaacaggccg tgagacggtc
      121 atgttcagta catttttacg tgctcaaatc tcgcttgcct gggttatcgg gccaccgctc
      181 gcttatgaac tggcaatggg gtttagtttt aaagtgatgt atctcaccgc tgccatcgca
      241 tttgttgttt gcgggctgat agtctggttg tttttgccat caatacaaag aaatattcct
      301 gtcgttaccc aacccgtaga aattttaccc tccacccata ggaagcggga tacgcggcta
      361 ctttttgtgg tctgttcaat gatgtgggcg gcgaataatc tctacatgat aaatatgccg
      421 ctatttatta ttgatgaact gcatctaacc gataaactgg ctggagaaat gattggtatc
      481 gctgccggtc tggaaattcc gatgatgtta atcgcaggct attacatgaa acgtattgac
      541 aagcgactat taatgctcat tgctatcgtg agtggtatgt gtttttacgc cagcgtactc
      601 atggcgacga ctccggcggt tgagctggaa ttgcaaattc taaatgccat cttccttggt
      661 attctctgtg gtatcggcat gctttatttt caggacttga tgcctgaaaa aataggctct
      721 gcgacaacgt tatatgcaaa tacttcacgc gtcggctgga ttatcgccgg ctctgttgac
      781 ggaattatgg ttgaaatctg gagctaccat gcgttgttct ggctggcgat agggatgttg
      841 ggtattgcga tgatttgcct gctgtttatt aaagatattt agctcaccag tctgggctaa
      901 gaaaaatcgg cgtggcgcaa acagagccat cctcgccatt tttgctatca tgcctgcata
      961 cataaacgac aaaacagtat gcagagggaa aaatgggttc caccagaaag gggatgctga
     1021 acgttctgat tgccgccgtg ttgtggggaa gttcaggggt ctgtgcgcaa tacatcatgg
     1081 agcaaagcca gatgtcgtcg cagtttttga ctatgacgcg tttgatattc gccggtttga
     1141 ttctgctgac gctgtcattt gttcatggcg ataaaatctt ttctattatt aacaatcaca
     1201 aagatgccat tagtctgctg attttttccg tggttggcgc gctaaccgta cagctcactt
     1261 ttttgctaac catcgaaaaa tcgaacgcag ccacggcaac ggtgctgcaa ttcctctcac
     1321 cgacgattat cgtcgcctgg ttctcactgg tacgtaaatc gcgcccgggc attctggttt
     1381 tctgcgctat tttgacatcg ctggtcggga cttttttatt ggtgacacac ggtaatccga
     1441 cgtcattatc gatctctcct gccgcgttat tctggggcat tgcctcggca tttgctgctg
     1501 cattctatac cacctatccc tcaacgctga ttgcccgcta tggcacgttg cctgtcgtcg
     1561 gctggagtat gctgatcggc ggtctgattc tgttgccttt ttatgccaga caagggacaa
     1621 actttgtcgt taacggcagt ttgattttgg cgttttttta tttgatcgtc attggtacgt
     1681 ccctgacatt tagtctgtac ctgaaaggag cacaattaat tggcggtcca aaagccagca
     1741 ttttgagctg tgcagaacca ttaagtagcg cgctactctc tttgctgttg ctggggatca
     1801 cgttcacatt accggactgg ctgggaacgc tgctgattct gtcatcggta attttgattt
     1861 caatggattc ccgtcgccgc gccagaaaaa taaatcgtcc ggcgcgggat gagtgagaat
     1921 taccagccag gcaccgcgcc accgttaaag atggtttctg ccgctttagc gacttcggat
     1981 gattgataag attgcagaaa ttccttcacg ttttctgcat tcttattatc ttcctgtgcc
     2041 accaaaatat tcacatacgg cgaattttta tcttcaataa atacgctgtc gtgcaccgga
     2101 gaaagcccgg tctgctgaat gtaagtggtg ctgataatcg ccacatcaac tttgggatca
     2161 tccagtacgc gcgggagctg cgccccctcg agttccataa tctgcaaatg acgaggatta
     2221 tcggttatat ccagcgcggt aggtaataac ccttttccct ctttcagagt aattagcttc
     2281 tctttttgca acagtaaaag cgcacgacca aggttggtcg ggtcgttggg gatcgccact
     2341 gtcgcgcctt cttttatttg cgccacggtt ttgatttttt tggaataacc cgccatgggg
     2401 aagacaaacg tatttcccac cgctaccagt ttataaccat gcgcctgatt atcttgttca
     2461 agaaagggtc gatgctggaa gacgttcgca tcaagttcgc catgatttgt cgcatcgttc
     2521 ggtagcaacg aaccgctaaa tcctaccagt tgaacatcga ggccatattt ctcttttgcc
     2581 acctttttag cgacttccgc gacatcttgt tctgcgccat ttataacgcc aactttaatg
     2641 tgttttgcat cgctgctact ctggtcgcaa cctgccagca gaattccggc cagcaataat
     2701 gcggcccctg tccgtagatg atgtgttgtc agtttcattt ttttatcctt ttattaatga
     2761 ttacctgact atagcggtaa ggtcgctgcg gtttaaaaaa cgaaacgcta tcgataagaa
     2821 taaaaaggaa taaaagtgga atataaagtc tggcacttct tactgaccac gcaagcgcgt
     2881 ttcgtacagc atgatgagag cgatgaaagc aaactacact tatgcttcat tcgatacaca
     2941 tttgtaaaag gttgaatcca tgaaaccaac gacactactg cttattttca cttttttcgc
     3001 tatgccaggc atcgtatacg ccgagtcgcc ttttagctca ctgcagtccg caaaagagaa
     3061 aaccacggta ttacaagatt tacgtaaaat ttgcacgcca caggcgtcat tatcagatga
     3121 agcgtgggaa aagttaatgt tgtcagatga aaacaataaa caacatatac gcgaggccat
     3181 cgtggcgatg gaacgtaata atcagagcaa ttattgggaa gcgttgggta ggtagaatgc
     3241 ccggatatgt aagaacagca acaactactg accaacgctt ctttacctga tatcaggatt
     3301 tcttcatttt cacctttgca gtaaccaaca atgcagtcag caacatcaat gtgccggaca
     3361 acatcaacgg cgaagtcaga ccaatattat ccagcgcata accgccgatt gccgcgccac
     3421 aggtattagc aagctgaata accgccacct gaatagaccc ggctttttct gcctgatcgg
     3481 ccagcgagcg ggtgatccac gttgaccagc cgacgggaac caatgcaaaa gttagccccc
     3541 agataatcgc cacgccggta gcaacgattt tatcgcttcc ccacaacgtc agtaccaacg
     3601 cactcacagc cagtattaac ggcgcgcctg ctaaggccag ttttaccgaa cgtttaagaa
     3661 tgaacgacga aagcgacgta ccaataaagc tgccgatacc aaaactcaac agcaccagcg
     3721 ttaagccatc cacgccgaat cccgctaggt tcatatacac cgggcgaata tacgtgaaga
     3781 aagcaaactg cccggcgaaa gacatgaaga tggcgatcat ccctgccatc acacccggac
     3841 gttgtaataa gcggaaaata ttttgtttct gatgcgaggg ttcgcctggc agtgaaggca
     3901 atgatttgat aatccagaaa atacacagca cgcccatcac cgccgccgca ttaaagacat
     3961 tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa
     4021 tagaaaccgc gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac
     4081 gcatggtcag cgacgccgac atcgcccaga acccgcccag cgccagcccc agacaggcac
     4141 gaccgattaa aagcaaactg aatgagttag caaaggaaac cagcaagcag gagagcgtca
     4201 gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt ggcctgaatt gtctgggtaa
     4261 taaacaaact ggcaaacatt gccacaaagg cggtcacggt caccgattgc ccggcaaccc
     4321 cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa actgacgggc aaaaactcaa
     4381 cgataatcag acaggcgaca caaaacgcca ccgagaaaac ggctgaccag ttcggtcggg
     4441 tgatggcatc cgcgccgcag ttttcggcaa taaattcact catggtgtta cccgtggcat
     4501 gtttcagggg aaaagccgac cactttaaca ttgaaatgtg tgacgcattt aacgtttttg
     4561 caacttttac ggtgttgctt gagctggagt taagatcgaa acggaacagg gcaaagatta
     4621 ccggatgcgg cgtgaacgtc ttatccaccc gacaggtcag gcgcggtctg taggctgata
     4681 agacgaaacg gtttcaatgc aaccagtgaa cgccctcggc aggctgaaag aacgcgttat
     4741 agcgcatctg aaaagcatta atatcctgca tttcaggttc ggtttcacgc agaaaaccca
     4801 gcgccagcct gacctgagca tcggtaatcg gcgcggaaag gcgggctttt tgtaaaatcc
     4861 ggtgccattg cagcaggttc tgttcgctat catcgacaat gacaatttgc cagatgagca
     4921 acacctgcgc ggcgttttgc aggtgcgact catccgggcg atgcaaataa cgtaaaaatt
     4981 cgtcaccggc tgactttccc atttgatcga ccatcgactc cacgggtacg acatcgatat
     5041 tcagccgatc gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc acccgaaaac
     5101 caaccacaaa cactaccgcc agcgtggcga gcattatcca gatcatgagt tctcctgcgt
     5161 aatcagaact catgatatct ggaaaccctc gccagttact aatccaccag aataatcttc
     5221 aatgcgaaca gagctgcgac caccaccaca cacaggttca gatcgcgcca gcgcccggtg
     5281 cacactttca tgatgcagta cgacataaag ccgagtgcaa tcccttcggt gatcgagaaa
     5341 gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg attcggtaaa atcatcccag
     5401 ttaacgcgcg ccaggctcga agtcatcagc acgccaacaa agattaacgc tccagcggtt
     5461 gcgtaaggag gaaccatcgc caccagcggt gagaagaaca taactaacag gaacataacg
     5521 ccgaccacga cagccgttag ccccgtgcga ccaccgactg ccacaccaga agtactttca
     5581 atataggcag taacagacga ggtaccgata aatgcgcctg ccaccgaact gacgctatca
     5641 acatacagcg ccttattcat attggggaat ttaccgttgc tatctattaa gcccgcttta
     5701 tcagttacac caattaatgt tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa
     5761 atgataccgg cgagttcaag tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg
     5821 ctaatatcag gcggaatgga atagacgccg ctaaaatgaa catcaccgaa aaataatcca
     5881 cagcaagacg tcaccacaat agaaaccagc accgcggcat gaaaatgacg tgatgacaac
     5941 acggttataa taaaaaaccc taaaatacct aacaacacgc cgtgagaact taaatcgcca
     6001 atcatcacca gcgtgtcttt attggcgaca ataacaccag tgttttttaa tcccattaag
     6061 gcaataaata atccgattcc actggtgata ccaatacgta aacttaatgg aatgttggag
     6121 atcatccagt accggatacg aaacagcgtg agcaaaaata gcccaattgc gccccagaat
     6181 atcgcgccca tcccggtctg ccaggagatc cccatcgccc ccacgaccac gaacgcaaag
     6241 aaggcgttca gccccattgc cggagccagc gccacgggta agttagcaaa tatccccatc
     6301 gcaatactgc cgataccggc aatcaaacag gtggtaacaa acaccacttt cgggtccatt
     6361 tgtgccgcgc cgaggatttg cgggttcaca aaaacaatgt acaccatggt taaaaaagtg
     6421 gtcatccccg caatcaattc tgtgcggacg gtggtcccat gctgaggtag tttaaataat
     6481 cgcgaaagcg tccctgattc attactcacg taatcggtat tgtcattatt catttttttg
     6541 tccattggag gagatttaat cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg
     6601 ataattatca catttcaatt gcacattaat agatattctt taataatctc gcgacgtttc
     6661 tttatgataa ataataatca aattgataaa atcaaaatga gaaaaatatg aataattcca
     6721 ttaaccataa atttcatcac attagccggg ctgaatacca ggaattgtta gccgtttccc
     6781 gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac ctgatcaatg
     6841 ccggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt gttggcgcag
     6901 aatacgctga tgctccggct ttgcagcgga ttgatgctcg cggcgcaacg gcggtgccag
     6961 ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc acttttgaaa
     7021 ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg
     7081 tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc
     7141 agtacttaca ggtcagctct tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca
     7201 gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc ggccttgcag
     7261 aaatgatgga ctaccctggc gtaattaacg ggcagaatgc gctgctcgat aaactcgatg
     7321 catttcgcta cctgacgctg gacggtcact gtccgggttt gggtggcaaa gaacttaacg
     7381 cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc
     7441 ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc cgcaatctca
     7501 acgcactggc accgttgatc aacgaattta acagcccgca atgcatgctc tgtaccgatg
     7561 accgtaaccc gtgggagatc gcccatgaag ggcatatcga tgccttaatt cgccgcctga
     7621 tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc
     7681 gccactttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc gatatcgtcc
     7741 tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg
     7801 atgcgcaaac cttacaggcg gaagagtcgg cgagactggc gctgtctgcc ccgccatatg
     7861 gcaataccat tgcccgccag cgactttgcc ctgcaattca cgcccggaaa acgctatcgg
     7921 gtcattgacg tcatccataa cgaattgatt acgcactccc gctccagcgt ctacagcgaa
     7981 aatggttttg atcgcgatga tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg
     8041 ctggctccgg cctgtggttt gctcggcggc tttggcctga atgaaggcgc gctggctgcg
     8101 acagtcagcc atgacagcca taatattgtg gtgatcggtc gcagtgccga agagatggcg
     8161 ctggcggtca atcaggtgat tcaggatggc ggcgggctgt gcgtggtacg taacggccag
     8221 gtacaaagtc atctgccgtt acccattgcc gggctgatga gcaccgacac ggcgcagtcg
     8281 ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat
     8341 gagccgttta ttcagatggc gtttctttct ctgccagtga tccccgcgct aaaactaacc
     8401 agtcaggggc tatttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa
     8461 taaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa
     8521 ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc attaccgcca ctatcgccat
     8581 caggcagata caaccaatcg cggcgatatc cagcgcggcg aaggtgcctg cccagccggt
     8641 aaggccgaat accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc
     8701 accaatcagg taagcaaagg tgcctttaat accatcggca gcgccaatcg cttttttagg
     8761 tacaaagcca acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa
     8821 cgcaaagaga gaagccagat agatatattc gttactggca tgttgataca caccgagcgt
     8881 ggcgataatc agcgccagcg cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc
     8941 agagagccag ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc
     9001 ctgaatcgcc accgctttag agagtttcag ttcctggaac gcgtatacgg ttgaccactg
     9061 gtcgatacca atacgtacca catagaggaa aatgttggcg aagcacagca gccagatcac
     9121 tttgtttttc agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt
     9181 ctctttgtcc tcttcgctga tctcctcgcc gaacagttct tcagctttgc cgaggccata
     9241 agattccggg gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat
     9301 cgacgggaag ataaacatgc cgatgacatg gccatcgaac aggtaatttg ccccgaacag
     9361 cgccacacct gctgcgcctg caccgccaag attgtgagaa atattccaga aaccgaggaa
     9421 tgtcccgcgt ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc
     9481 ggtactctgg aaaaagccgc ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga
     9541 gccgctgccc atactggcac tgaagcccag catacaaatg gcagagagga taagcatgaa
     9601 cggcaggaat tgtttggtgt ttttgccgtc ggcgtagtat gaaaccaacg ttttacccac
     9661 gccataagtg atggagaaac ccaggccgat catccccagc tgcgtcatgc tcaacccgta
     9721 ggtcgaaatc atatcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag
     9781 gtagccgata aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg
     9841 cacttcgagc ggaaggtcca gggtcggctt gcgaacctgg tttaggaaag ccagcatggg
     9901 ttactcctga aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg
     9961 cctgagatag cgtccaagaa aaacctggaa aatttcttag ttttgccgga tatgaagtga
    10021 aaaggtgaga tgcatcacgc ttcacgcggt gtctgggcgt tcaaaaaggg cagtaacagc
    10081 agtgcggaaa tcccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta
    10141 tcgagcactt tcgccagcgg ccaaccagca agcgacgccc ccagataagc aaacaagccg
    10201 acaaaccccg tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccatacca
    10261 attaacatct gcgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc
    10321 acgtagctgg caaatggcat cagccacagc gagccgactg aaagcaaaat tccggcggcg
    10381 aaaatcaaat tcatcggccc acggttgccg ttaaacaatt tgtccgagcc ccaaccggct
    10441 accagcgcac cgataaatcc gcccagttca aacatcgtca ctgccgtatt cgccgtgacc
    10501 agatcgacgc ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccgg
    10561 accacataga ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca
    10621 tatttggtga ggatctcttt acgcgtcaac cctgcccctt cttgttgttg agcaatttcc
    10681 agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgt
    10741 agccgccagc agagaaaaat ccccacgact atcgccatac aaccagcaat catcatcccg
    10801 gcacgccagc cgtaatgcag cgcgctcgct gccatcacaa tgggaatgag tgcgccgccg
    10861 acgttatgcg ccgtgttcca taatgcccac caaccgccgc gctcggtacg tgaataccag
    10921 gccgttaaca gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc
    10981 cagagtacgg caaacgccca tagcgacgtc gagaagccaa acagaatgtt catgatgccc
    11041 gtggcaataa gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg
    11101 gagacaaact tcgacacgcc gtaagtaatg taaaacaggg tcgctaacag gccgatatcg
    11161 ctacggctga gcacgccgtt agcaaggatt tctggtaccg cggcgttaaa acttttccgc
    11221 gtgaagtaaa acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag
    11281 cgataacggg catcaatttc atgtttatca gtcattaatg gcgcatcggc aggcgctttc
    11341 agaaacggca acatcatcga accttagaca taacgttgag gtagagaaac gctgacacgc
    11401 gtgccgtgca gacaggaaat gtgtaatgtg ccgcccagcg ccgttacgcg ctcgcgcatt
    11461 ccggtgaggc caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca
    11521 atcaccagca tcaaccgttc atcctgctgc cagccttgca gcgtaaccgc gctggcatcg
    11581 gcatgtttca caatgttgtt cagcccttcc tggcagacac gaaacagcgt cacgcgctgg
    11641 ttttcgctta atgctgattc atcgatacgc cattcgagat ggctgacaat gccgcgccct
    11701 tccagctcca tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccagc
    11761 tggcgcggac gtaaccgtcc taacaaacgg cgcaccgcgt cgtaaacgcc cagcgacagt
    11821 tgttcgatga gctgcccgct ctgcttcaca ctggcgttat ctgccgccag ccgctgaaca
    11881 atgcccgcct gagtacgaat agcagtgatg gtctgaccga tatcgtcgtg cagctcacgc
    11941 gccacgtcgc ggcgcacgct ctcttcggtt tctaacaaac gttcagccag atgctgattg
    12001 cgcgccagtt ccttttgtag cgactggtta agttcacgca accgctggat gcctgcgccc
    12061 agtaacaacc ctgtcagact ttgcaccagc aacgagagta ataaatccac cggatgatcg
    12121 cgccaggttt gactggcgat cagcgcgatg gcgttcatca acgtcgcaat cagcgctcct
    12181 tgccagccat agtgccaggc cagcgcgata atcggcagcg ccagacagaa tggcgtaaag
    12241 cgcgacagtt cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag
    12301 taccagacca gatgccgccc gcgccagttg atgggctgag aaaccagtga cgggccgagc
    12361 ggcagccagg tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc
    12421 agaccgccag ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag
    12481 ggcagcgact gcaacaacgc cgccgccgtt aacgccgcac cctgtaacag caaggtgcgc
    12541 cagtcacgct gatggcgata gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta
    12601 ccgatcatca ataacggaaa atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc
    12661 agccactccg cacccagcaa tacgggccag tatccgcgcg ggcattgcag cattagcccc
    12721 agacgcagac caaacggaaa taacagcacc gccatatcag ggcgctcaac cagatgcagg
    12781 ctgatactcc ataggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta
    12841 attaagcggg agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagctcta
    12901 cgtcgttact gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg
    12961 gtgacaagcc cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttccg
    13021 ccacctggcg ttcacgtttg gtcagcgggt cctgacgacc ggatgccagt ttaatggcaa
    13081 tatccggcgt cagataacag ccgcccgtgg caaccgtatg caccgcagca atgagttcat
    13141 ccgggctaca gcgtttggaa agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca
    13201 gcgcaggact gtcgtgaacg gagagcataa tcgtcgccat acctttcggc agctggctta
    13261 gcagctccag accggagata tcgggcatgg agatatcgca aatacacacc tgcacaccgc
    13321 gccccggcag ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat
    13381 caggttccag ccccagcagc tgcgcaaagc cggagcggac gatgaggtga tcgtctataa
    13441 gggcaacggt gatcatggtc ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga
    13501 taagcgcgat gttgttcaag ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa
    13561 catcgtcgga tcggactgat tacgctgcac tttcacgaca tcttccagct tatcgatttg
    13621 gcttatcatc tgctccagac gctggtcgtc attgaccagt agccagatat ggcttttgtc
    13681 gctgtcctga atcggcagac aaagaatgcc ttcaacgtta aaagcgcggc gggaaaaaag
    13741 gccacaaacg tgggtcatta cgcccggatg gttgcgaacg gtgagctcca gaattacgtt
    13801 gttatgagtt gtgttttgca tggcttattc ccccaccatt tcagtattcg ccgcacctgg
    13861 cggcaccatc gggtaaactt tttcttcggc atcaatgcgc acatggatca gcgccgggcc
    13921 agggcgattg atgatttcct gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca
    13981 ggtttcgagg ccgaatccgg cggcaatctg cataaagttg attttgcccg gataggtggc
    14041 ggcaaaaacg ccttgctcgt agaacagact ctgttgctga tgcaccagcc ccagcgcttc
    14101 gttgttcatc agaatgattt tgacatccag ctgattttca ctggcggtcg ccatctcctg
    14161 aatattcatc atcaggctgc cgtcgccgga gaaacacaac actttgcgat ccgggttcgc
    14221 cagcgccgcg ccaatcgccg caggcaggcc aaaacccatc gtgcccagcc caccggaggt
    14281 cagccactgg cgtgggcgat tgagcggata agcttgcgcg gtccacatct gatgctgacc
    14341 cacatcggtg gtgataatcg cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc
    14401 gtaatggctt aatggatcgc acgctttcgg gattggacac gggaattcgc gctgcaaatc
    14461 cgctaccaac tggtgccact ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc
    14521 cagcacgtca tcaacatccg cctgaatcgc cacatgcggc tgcttgattt tacccagctc
    14581 tgcgcgatcg atatcgacat gaataatttt ggcattcgga cagaactgct cggttttgcc
    14641 aatcgcccgg tcatcaaaac gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat
    14701 atagttggta ctgcgcacgc cgtgcatccc cagcataccc agcgacaacg gatgcgcttt
    14761 tggcagcatg cccagcgcca ttaaagtcat ggtggtaggc agttgcgctt tctccgccag
    14821 ttcacgcacc cgcgcaggcg cattgatcac accaccaccc agataaagca ccgggcgttt
    14881 ggcagcgtta atcattgtag ctgcgtcacg aatgctttct tcgctaaagg cgggtgcagc
    14941 ggctttttct gccaccgcgg gctgagcttc aatctcaaaa accgccgttt gtacatcctt
    15001 aggaatgtct atccacaccg ggccagggcg gcctgattgc gcaatgcgga aggcatcgct
    15061 catgacctgt gggagctctt cgatatgtct gaccagatag ttgtgtttgg tgatggggat
    15121 agagatgccg taggtgtcca cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac
    15181 ctgaccagtg atgcaaatca gcgggatgga gtccagccgc gcatcagcaa tggcggtcac
    15241 caggttagtc gcacccggcc cgctacaggc catacagacc gccggtttac catcggtgcg
    15301 cgccattccc tgagcgataa accccgcgcc ctgttcatgg cgagccagaa tatggcggat
    15361 ttgcgtgctt tggcttaagg cgtcgtaaac aggcaggata gaaccgcccg gaatgcccgt
    15421 cacaatctta atgccctgct gttccaggaa atgaacgata aattctgcgc cggtaaagcg
    15481 cttacgcgtc gatgttgtgc ccgaacttgc catgctccag tccttttctt ctgggccgac
    15541 tttccgggag gtgcttaaac gaaaaacccc gcccggtttg cgccggcggg gttttggaat
    15601 cgtgtgttgt tccagtccct acggcgcatt gccgacgacc accaccacac gcacgacgac
    15661 cactgcggcg gatggcgcag ttggtagtag ttttgcgttg aacatggaag tagtcattgg
    15721 ggaccttgtt ggttttgtgt ttaacaatat ttatacaagc acagctttac aggggagaca
    15781 atggaaaatt tttcagcaag ggaaaattga ggggttgatc acgttttgta ctgaattgca
    15841 gataacaaaa aaccccgccg gagagaggtt tcgtcagccg cctgcggctg gtaaccgcaa
    15901 agcacactgt attatgtcaa cactgaaagt atacgtgttc cgcgcagaac gcgcaatttc
    15961 ggcacgaatt ttgacgtatt tagtgcatag ttgagtatcg atcacagttt gcgttttgtc
    16021 caaatattac tgtttattta tacagtaaac ttctataata tcactgtacg caatgtgtta
    16081 tgcgggggcc gcatcgttac ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca
    16141 gcgccttaac cccgcgtgag cacactgtgt tatgtcaaca agcacaacgt ttctccttga
    16201 gataccgcgt gcacaacagc tggcaacagg cagcggaaag gtacgtcagc tggcagtgct
    16261 cctgaaccac aggagacgcg tatgagcctg gtggatatcg ccattcttat cctcaaactc
    16321 attgttgcag cactgcaact gcttgatgct gttctgaaat acctgaagta attcagattc
    16381 aagtcgcacc aaaggggagc gggtaaccgc tcccctttta tatttagcgt gcgggttgga
    16441 gtcggatgcg atgctgacgc atcttatccg ccctaccatc cctcccggca acatttattg
    16501 ccgcttttgt ttacatattc tgccgctaaa caattcccca ttcctggcgt atatctggct
    16561 aacattcatc aatgtgatag attcctctcc cgcatttatg ggaatgcgta gtgacttatt
    16621 ctaattattt ttataaaagc atccgtgata atgaaaaggc aaagaaacgt caatttgtta
    16681 ttgatgttgg tattactcgt ggccgtcggt cagatggcgc aaaccattta tattccagct
    16741 attgccgata tggcgcgcga tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc
    16801 gcttatctgc tgacttacgg tgtctcacag ctgttttatg gcccgatatc cgaccgtgtc
    16861 ggtcgccgac cagtgatcct cgtcggaatg tccattttta tgctggcaac gctggtcgcg
    16921 gtcacgacct ccagtttgac ggtattgatt gccgccagcg cgatgcaggg gatgggcacc
    16981 ggcgttggcg gcgtaatggc gcgtactttg ccgcgtgatt tatatgaacg gacacagttg
    17041 cgccatgcta acagcctgtt aaacatgggg attctcgtca gtccgttgct cgcaccgcta
    17101 atcggcggtc tgctggatac gatgtggaac tggcgcgcct gttatctctt tttgttggtt
    17161 ctttgtgctg gtgtgacctt cagtatggcc cgctggatgc cggaaacgcg tccggtcgat
    17221 gcaccgcgca cgcgcctgct taccagttat aaaacgcttt tcggtaacag cggttttaac
    17281 tgttatttgc tgatgctgat tggcggtctg gccgggattg ctgcctttga agcctgctcc
    17341 ggcgtgctga tgggcgcggt gttagggctg agcagtatga cggtcagtat tttgtttatt
    17401 ctgccgattc cggcggcatt ttttggggca tggtttgccg ggcgtcctaa taaacgcttc
    17461 tcaacgttga tgtggcagtc ggttatctgc tgcctgctgg ctggcttgct gatgtggatc
    17521 cccgactggt ttggcgtgat gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt
    17581 ttcggtgccg ggatgctgtt tccgctggcg accagcggcg cgatggagcc gttccccttc
    17641 ctggcgggca cggctggtgc gctggtcggc ggtctgcaaa acattggttc cggcgtgctg
    17701 gcgtcgctct ctgcgatgtt gccgcaaacc ggtcagggca gcctggggtt gttgatgacc
    17761 ttaatgggat tgttgatcgt gctgtgctgg ctgccgctgg cgacgcggat gtcgcatcag
    17821 gggcagcccg tttaagcgca cgtcaccgca gcatcgtcat cagctccatg ggagaacgat
    17881 gctgctttat cagatcacgc atcacccgca tatgcggtgc ggagtaagaa taaaacgcct
    17941 gatagcccgc acaaagcacg ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat
    18001 gccagtcgca cgcctggcag tcacggcgcg gttgcgggtt ttcgctgcca caccattttt
    18061 gtaaggtggc gtcaaaaagc tcaatcgaga tacgactgat atctgcccgc acccaacact
    18121 caaacagggc aattaaaaat ttcccccaca gctctgctgt caccgaagcg gccgtcagat
    18181 cgccccggtc atcaaattca agatggggaa tgagctgtac ggagcggatg ccccgctgtt
    18241 tgaaattgtg ataaaaatcg gcaggttgca gagcatcctt gtactctaca accagcttat
    18301 gcctgtcctc ttcagcatca atgacctgac ttcctgtcat gcgactctct caataaatac
    18361 gataagttga tgtatatgcg ttacagcaaa cagtattgcg agagataaca ccgcgaggga
    18421 gatcaaaaat ttgtcacgga ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat
    18481 taaattgcga ttacgggtgt agtgccagag gatcagcgcc acgatggcga ggatgccaat
    18541 cgaaatccag aataacatgc ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa
    18601 cgcgccgtag cccagcatgg tacgaaacca cgccagtgat gtgcgctccg gctgcagccc
    18661 tgggtcggca atgcgtctgg ctttacggct atccggcata caacaccagt cccataacga
    18721 tcaccgccac aaccatcaat attaagctga tgattaataa gctgttggtg tagggcaggt
    18781 cctctttcag acgcatcgct ttttcattgc gtaaccagcg caggtagcca tacattgcta
    18841 acccgccgga aaacagacac aacaacaacg ccagcagttc acgaatgacg ggcgtggcga
    18901 aatctggcgc aagttgatca agcccaaccc ccgccgccag aaatcctagt gcggtacgga
    18961 tccacgccag aaaagtacgc tcattagcca gcgagaagcg gtaatcgggc gcttctccaa
    19021 ggcgggaaat tttcattgtt gctcctttgc aatttcacta acgctctatc tttagcggtt
    19081 ttttttaaga atttcgtccg cccgtttcct gatatgcttt tgccatccgc tacaaggagg
    19141 tcagatgggt atcatagcgc aaaataaaat cagttcattg ggcatgctgt tcggtgccat
    19201 cgctttaatg atggggatta tccacttttc attcggccct ttttccgcac caccgccaac
    19261 gcttgaaagc atcgtggcgg acaaaacggc tgaaattaaa agaggtcttc tcgccggaat
    19321 aaaaggcgaa aaaattacca ctgtggagaa aaaagaggac atggatatcg ataaaatcct
    19381 cgatcaaagc ggtatcgcac tggccattgc tgcgctactt tgcgcattta ttggcggtat
    19441 gcgtaaggaa aatcgctggg gcattcgcgg cgcgctggtt tttggtatcg gcatcgtgtg
    19501 cagcattttg ctgatattcc tgattttttc cttcctgacc ggcggttcgc tggtttaagc
    19561 agcctgaata atgcccgccg ggtgatcatc agcgggcatt atcgtcagaa tttctgtttt
    19621 ttctcctcca ccttcactcc ttgcgtcggt aggccggtgt catagtcgcg taccactggc
    19681 gaatagccat cttgtgggcg tgggcgaaac gcccccatcc agcgcggtcg tgcatcttta
    19741 cgccacggac gcagactcca ttggtaactg cggaacggat cgcgaatttt gtccatgtaa
    19801 tccaataagg cgtcatgcat tttgctgcga acgtctgcaa aacggatatc atcgatcagg
    19861 ttatgcattt catttgggtc attacggcga tcgtaaagtt catcactggt gaagaggttg
    19921 agcaccagtt taaagtcatc cgtcacccag caacgcaccg gaataaaacc gccaaagctg
    19981 tcatgctcaa tctcgtagcg gttaaattcc accatcacgc cgcgtggctc tttcacggca
    20041 aggatatttt cccccggcag aatctctggt ttttcaatat ctgccagcgc catcattgtc
    20101 ggcagtaaat caatatgact gactggcgta tcgacctgtc gccgctcccc ttgcggcgaa
    20161 cggatgatca gcggaatacg ggtgatgtcg tcatacatcg ccgctccttt actgatcagc
    20221 ttatgtgcgc ccatcatttc accgtgatcg gaggtataaa tgacccacgt attttcacgt
    20281 tgctctgccg ttaaggcatt gatgacacgt ccgatttggt catcaacaaa gtcattgcag
    20341 gcaaaataga gcggatggtg ataaagcccg tcatcaccga ctggcgatgg catcgcctgc
    20401 gcccataagc ggtgatgttc cggtttgttc gccaggtcat cctgtgattt ctcgccaagt
    20461 tcgtagtaaa aatcagcgta tttctctaaa tactccaccg gacaggtgaa cgggtgatgc
    20521 ggctcatcat acgaaaccac catcaggaag ggttcctcgg cgcgcacggg ctgttgcaga
    20581 aaatctaccg ctcgattgct gatgcggtgc gcccaggtga aggtttcgtc aatatggttc
    20641 gcctgtaaat cttcgacgct gtttaggcca ttgcgccaca ggctgatctc tttttccgtc
    20701 agttcgctaa gatagttcgc cccatcaaac cagtaatcag catcccactc cggcggacac
    20761 tcgccagtgc cgaaatagtc atgaccgtcg agatgccatt tgccgatgta acaggtgtga
    20821 tagccggcat ccttaaagta gcgccccata g
//
LOCUS       DADLSE010000063        20848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLSE010000063 DADLSE010000000
VERSION     DADLSE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.063"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(48..782)
                     /gene="araH"
                     /locus_tag="JN692_002296"
                     /pseudo
     CDS             complement(48..782)
                     /gene="araH"
                     /locus_tag="JN692_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
     gene            complement(794..2308)
                     /locus_tag="JN692_002297"
     CDS             complement(794..2308)
                     /locus_tag="JN692_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HAY7264598.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2378..3367)
                     /locus_tag="JN692_002298"
     CDS             complement(2378..3367)
                     /locus_tag="JN692_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7264599.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4164..4667
                     /locus_tag="JN692_002299"
     CDS             4164..4667
                     /locus_tag="JN692_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7264600.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4747..4998)
                     /locus_tag="JN692_002300"
     CDS             complement(4747..4998)
                     /locus_tag="JN692_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7264601.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN692_002301"
     CDS             complement(5113..5199)
                     /gene="azuC"
                     /locus_tag="JN692_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7264602.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5462..5785
                     /locus_tag="JN692_002302"
     CDS             5462..5785
                     /locus_tag="JN692_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264603.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5956..6453
                     /gene="ftnA"
                     /locus_tag="JN692_002303"
     CDS             5956..6453
                     /gene="ftnA"
                     /locus_tag="JN692_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7264604.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6490..6729)
                     /locus_tag="JN692_002304"
     CDS             complement(6490..6729)
                     /locus_tag="JN692_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7264605.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            6919..8130
                     /gene="tyrP"
                     /locus_tag="JN692_002305"
     CDS             6919..8130
                     /gene="tyrP"
                     /locus_tag="JN692_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7264606.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLATAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN692_002306"
     CDS             complement(8192..8857)
                     /gene="yecA"
                     /locus_tag="JN692_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7264607.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(9053..9139)
                     /locus_tag="JN692_002307"
     tRNA            complement(9053..9139)
                     /locus_tag="JN692_002307"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9103..9105),aa:Leu,seq:taa)
     gene            complement(9152..9225)
                     /locus_tag="JN692_002308"
     tRNA            complement(9152..9225)
                     /locus_tag="JN692_002308"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9191..9193),aa:Cys,seq:gca)
     gene            complement(9280..9355)
                     /locus_tag="JN692_002309"
     tRNA            complement(9280..9355)
                     /locus_tag="JN692_002309"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9320..9322),aa:Gly,seq:gcc)
     gene            complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN692_002310"
     CDS             complement(9507..10055)
                     /gene="pgsA"
                     /locus_tag="JN692_002310"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7264608.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN692_002311"
     CDS             complement(10112..11944)
                     /gene="uvrC"
                     /locus_tag="JN692_002311"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7264609.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN692_002312"
     CDS             complement(11941..12597)
                     /gene="uvrY"
                     /locus_tag="JN692_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7264610.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12893..13069
                     /locus_tag="JN692_002313"
     CDS             12893..13069
                     /locus_tag="JN692_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264611.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            13056..13280
                     /locus_tag="JN692_002314"
     CDS             13056..13280
                     /locus_tag="JN692_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7264612.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN692_002315"
                     /pseudo
     CDS             complement(13313..13933)
                     /gene="sdiA"
                     /locus_tag="JN692_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN692_002316"
     CDS             complement(14163..14915)
                     /gene="yecC"
                     /locus_tag="JN692_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7264613.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(14912..15580)
                     /gene="tcyL"
                     /locus_tag="JN692_002317"
     CDS             complement(14912..15580)
                     /gene="tcyL"
                     /locus_tag="JN692_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7264614.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15595..16581)
                     /gene="dcyD"
                     /locus_tag="JN692_002318"
     CDS             complement(15595..16581)
                     /gene="dcyD"
                     /locus_tag="JN692_002318"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7264615.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(16686..17486)
                     /gene="tcyJ"
                     /locus_tag="JN692_002319"
     CDS             complement(16686..17486)
                     /gene="tcyJ"
                     /locus_tag="JN692_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7264616.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(17574..18125)
                     /gene="fliZ"
                     /locus_tag="JN692_002320"
     CDS             complement(17574..18125)
                     /gene="fliZ"
                     /locus_tag="JN692_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7264617.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(18171..18890)
                     /locus_tag="JN692_002321"
     CDS             complement(18171..18890)
                     /locus_tag="JN692_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7264618.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNEREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(19055..20707)
                     /locus_tag="JN692_002322"
                     /pseudo
     CDS             complement(19055..20707)
                     /locus_tag="JN692_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
BASE COUNT         5212 a         5366 c         4829 g         5441 t
ORIGIN      
        1 gcaaacatgg actggcaaaa gtgtagcggt attaagcgca gctatttagt catacgtgaa
       61 gccagaataa ttccggctat cgcagatacc aggccagaga gaacaaagat aataattttg
      121 gtgcgaacaa ccggtacacc cgccagacgc gcagcctctt cgttcccgcc aatcgccagg
      181 gtattacgac caaaggtggt tttattcagc agcaaaccaa agataatcag acacgcgacg
      241 gtgagccaga ttggcgcagg cagaccgaac cagttagcgt aaccaagggc aaagaagctt
      301 tcatcttcga taccgaccgc tttaccgtct gaaatgatgt acgccagacc tcgaacaatc
      361 tgcatcgttg ccagtgtcgt gatcagagca tttattttca gtttggcgat aacaaagcca
      421 ttgaccaggc cacagagaac gcccagcgac aaacccgctg ccacgccaat ccacaggctt
      481 tcagtcaggt tgataaccac cgccgtggtg acacccgcac aggcaattac ggaggcgaca
      541 gaaaggtcaa agtcaccgga agcaaggcag aacaacatgc cacacgccac catccccgac
      601 atggaaattg ccaggcccaa ccctttcata ttaatgaagg tggcaaaatt tgggacaaaa
      661 atggcacagg cgataaagag caccgcaaac accaccagca tgccgtactg atcccagata
      721 cgcccgaagc tgaatgacga cttaggtgcg ccagaccccg atgtagaaac agaagacatc
      781 atactctcct tactcaggca actgcctggc tgactttagg catcgcaagg ctcagtgcct
      841 gacgctcatc tgcctgctcg tgtaacaatt caccggcgat ttcgccttcc cgcatcacca
      901 caatccggtc ggcaacgccg aggacttcag gtaagtcgct ggaggcaaac agcaccgcca
      961 cgccctgcgc cgccagcgca taaattacgt tatatatttc gtgcttagcg ccaacatcaa
     1021 tgccgcgcgt aggttcatcc agcaaaatga ccttcatctc ttccgataac cagcggccca
     1081 gaatggcttt ttgctgattt ccgcctgaga gattcatgat cagttgctcc gcgcccggcg
     1141 ttttgatgtt gagcgaacga atgtgctgat cggcattgtt ttcttcccaa ctgttgttga
     1201 ttacacaacc gccgagcaca tgtttgcgtc tggcactgat gttgatattg tcgcgaacgg
     1261 agtgcacggg aataatgcct tccgctttgc gagcttccgg gcagagcatc atgcctgcgg
     1321 caatggcgtg gctcggttta cgaatatcga tcggctgttg gtcgatataa acctgaccgg
     1381 cggtgatttg cgtcccgcca aacaagcctt tcattaattc gctacgcccc gcccctacca
     1441 gaccaaacag cccaacaatt tcaccactgc gaaccgccag acttattggc gtacgcacgc
     1501 ctggtgcttt cacagcatca agacgcaggc gttcctcgcc ataactacga ggttgccagc
     1561 cgtagatatc gccaatgtcg cgcccgacca tcgcctgcac cagcgcgtcg tggtcaacct
     1621 gctgcatatc ggtaaaggtt ttgacataac gtccatcttt aaagacagtg atggcatcgc
     1681 tgagggcaaa tatttcttcc atacggtgag aaacgtataa gatcacccgc ccctcttttc
     1741 gcagttcacg aataacgcgg aaaagattgc cgatttcgcg ggcagaaagg gagctggttg
     1801 gctcatcaaa ggcgataatt ttagcgttac gcgccagcgc tttggcgatt tcaaccatct
     1861 gccactgacc aatggagaga tatttcagcg gcgtgtccgg gtcaatatcc ataccaagat
     1921 gtttaagttg taaacccgcc tcataattca gcaatgagcg attcacaatg ccgcctttat
     1981 gcggcagctg gccgagatag atgttttccg cgacggtcat ttccggcacg agatgcagtt
     2041 cctggtaaat aatcgccaca cccgcgttaa gtgctgcggt cgtgtcggaa aaggacattt
     2101 cctgcccatt aatcactaca gaacccgtgg ttggcgcata gttgccgctg aggattttta
     2161 agagagttga ttttcctgcg ccattttcac ccatcaacgc atgaacctga ccggcatagc
     2221 agtcaaaact aatatccgtc agcgccttaa cgccgggaaa cgttttaccg atgccgcgaa
     2281 atgagagata cggggtagac tgttgcataa cgtctccgtg aatcactggt cgttcacacc
     2341 ccctctgcat catgcagagg ggaatttttc cggcaaatta cttaccgcct aaaccttttt
     2401 tctccagttc ttctttaaag ttgtcacgcg tgatcagtac cacgtctgta acttcggtaa
     2461 attttggcgt ttcaacgtct tttgctaccc agttgtaaag catttcgctg gatttatagc
     2521 catgtacgtc cgggcttggc agcagggaac cgtagaagcc ggttgcctgt gctttagaca
     2581 gttcgcttac tgcatccaca ccgttaatac caataccgat gatatcggct gctttaaagc
     2641 cctgaccttc ggttgcacgt acgccgccaa gtacggtgct gtcgttcata ccgacgatca
     2701 gccaatgttt aacttccgga tgttgaacca gcattgagtt ggcagcgtca aatgcccccg
     2761 ggatgtcgtt agatttggta ggtacctgat aaatttgttt ttccgggaat cctgcagctt
     2821 tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt atccagttcg ttggcggtaa
     2881 tcgccatcac tgcgctttct ttgacatccc agccacgttt ctgcatctct ttatacagtt
     2941 cctggccctg acgttcgcca attttagtcg ccgccatcat caccagagga acggtatcca
     3001 ttggcttacc tttggcgtta acaaactggt catccactgc aatgactttc atatcgtagc
     3061 cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc cggagtacaa ataacgaaac
     3121 cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa tgttttttcg ccatccggca
     3181 cggcaatctt aataacctca aaccctaaat ccttcccggc tttatcggca aacttccatt
     3241 cggtctggaa ccacggctct tccggttgct tcaccagaaa accgagcttc aggttctccg
     3301 ccatagcgga ttgtgacata acggctgcca gaccaatggc tgccagggct ttagtaaatt
     3361 tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag ggcaaaaacg aatgacattc
     3421 gttaaattaa tcggaaaaca aagcattacc ttttatctaa aagataagtg accgtgttga
     3481 catagtttta gcgagaaatt aattctccat aggagagcaa tatcacatcg cagaattaca
     3541 gtgagaacgt gcataaattt agcgggaaaa gacataagga aaagccaatt tgtcagacaa
     3601 attgttgaat gcacagcaga ttaatccata agattagcct ggaaatcctt gttgtctttg
     3661 gtacccatgc gggatgtctt ctttttaacc agtcaatagg ccgcattacc tggcgttgag
     3721 tttttgaaat ggtgtaataa ccacaactca aagatgtgga aaatgcgcgt cattcatttc
     3781 gccattaatt atcactgtgc tcattaatta acagaacacg tataatgaga gccatctcgc
     3841 aaaaatgaaa aaacgtttta taaaatcatc acttcatcat gaattcaaat tcatcgatta
     3901 atatcaacaa gatacaaaaa gcactatcat taaaattcat tgcatttaca ttgatttcac
     3961 caatgaaatg taaaaatata taaacttgat gatttaagca ttttcttata accgttcaga
     4021 cgttattctt atttcagatc atcgtcagaa ttgactccac gatcacattt cggaccggca
     4081 gaaaggaatt attctgcaaa cagtaattat ggtgttttga tttatcttgc acctctccac
     4141 ttctggatat aaggatatta ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc
     4201 aaatgaaccg cgagttttac gcatccaatc tctaccttca cctgagtaac tggtgttctg
     4261 aacagagtct gaacggcacc gccactttcc ttcgcgccca ggcacagagt aatgtgaccc
     4321 aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc tacccccatc gttaaagcca
     4381 ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact gttccaaaaa acgatggaag
     4441 aatacgagca acgttcaagc acgctggcgc agttagccga tgaagcgaaa gaactgaatg
     4501 atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga acagcagcat gatggcctgt
     4561 tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc
     4621 agaccgacca acatgttctg aatgtcgtgt cacaccagct gcattgatca tacatcggcg
     4681 ctaatacatt gcgccgatga agtttttgag aaaccgctgc ctcatctgtt tgaagcagcg
     4741 gtttttttaa tgggattcac cctgtggggt aaacttgagt tcaatcagcg cgatggcttt
     4801 ttggattgcc cgcatggtga ccgggtctgc ggcagcggga tgatgagtaa agtcgatatt
     4861 cttcagctga ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc
     4921 cagaatttgt ttgataacca actggcaagc taccacatca gaaaccagtt cctgatcggc
     4981 attcagcggc tgggacatcg taaactcctg atagcatttt gaaagccgtt atagtagcgg
     5041 cttcacatct tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga
     5101 gcaggttttt tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata
     5161 gttattgaac atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt
     5221 tatacaggag ttattgtctt tgcgcttata atatgacaac catcacaaaa atcaatcttt
     5281 atgtgataca aatcacataa ataccccttt aatgttataa aaatgataat caaaaaacag
     5341 cccccctatt tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg
     5401 gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc tcttactctt ctggctggac
     5461 catgagactt ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg
     5521 ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca
     5581 ggcttttttg cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc
     5641 atgccagcag gaaggctata cccacgcgat cccctttggt cagccagtag gcaactgtag
     5701 cctttttgcg ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca
     5761 ttccgccttc cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa
     5821 agaaatagcg gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt
     5881 taccttttgc aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct
     5941 ttgtggagca ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac
     6001 ctggaactgt actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc
     6061 ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg aagagatgac gcatatgcag
     6121 cgtctgtttg attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct
     6181 ccgtttgctg aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa
     6241 ttaatcaccc agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca
     6301 acatttaatt tcctgcagtg gtatgtttct gagcagcatg aagaagagaa actgttcaaa
     6361 tcgattattg ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac
     6421 aaagaactct ctaccctcga cacacaaaac taatgctctc cggcggcagg tttctgccgc
     6481 cgtttcaaat cagtgacggc aaatcttact ctgatcggtc gaaaaacctt cttctgaagg
     6541 tataaatttg ccttttgctg ccagaaacgc taccagctct cccgctgtca tcccttctgc
     6601 cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct ttaatcgcag cttccagact
     6661 ggcatgcgta tattgctcgc ctgattcaat catcatattt aacacttcat gaccgtgaat
     6721 agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg attacctctt atgaagactt
     6781 tgcgccagcg caggactgaa tgcttttatt gtacatttat atttacacaa tatgtaacgt
     6841 cggtttgacg aagcagccgt tatgccttaa cctgcgccgc agatatcact cataaagatc
     6901 gtcaggacag aagaaagcgt gaaaaacaga accctgggaa gtgtttttat cgtggcggga
     6961 accacaattg gcgcaggcat gctggcaatg ccgctggcta cggccggtgt tggttttagc
     7021 gttacgttaa tcttgttgat tgggctttgg gcgttgatgt gctacacggc gctattactg
     7081 ctggaggtgt accagcatgt tccggcagat accggtctgg gcacgctggc aaaacgctat
     7141 ctgggacgct acggtcaatg gctgacgggc ttcagtatga tgttcttaat gtatgctctg
     7201 actgcggcat acatcagcgg tgccggtgaa ttgttggcct ccagcatcag cgactggaca
     7261 ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca cttttgttgc cggtggcgtg
     7321 gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt ttctgttcag cgccaaaatt
     7381 atttttctgg tggtgatgct ggtattgctg ctaccgcata ttcacaaagt gaatctttta
     7441 accctgccgt tgcaacaggg gctggcactg tctgcaatcc cggtgatttt tacgtcgttt
     7501 ggttttcacg gtagcgtgcc gagtattgtc agctatatgg atggcaacat tcgtaagcta
     7561 cgctgggtgt ttataatcgg tagtgccatc cccctggtgg catatatttt ctggcaggtg
     7621 gcgacgcttg gcagcattga ttcaacaacc tttatgggat tgctggctaa tcatgctgga
     7681 ttaaacgggc tgttacaggc gttacgcgaa atggtggcct ctccgcatgt tgagctggcg
     7741 gtacatttat ttgctgattt agccctcgcc acgtcatttc tcggcgttgc gttaggctta
     7801 tttgattatc tggctgattt gtttcagcgt tcaaataccg ttggtggacg attgcaaact
     7861 ggtgcaatta cctttctgcc gccgttggcg tttgcactgt tttatccacg aggatttgtg
     7921 atggcgctgg gttacgccgg tgtggcgctg gcggtactgg cattgattat cccttcacta
     7981 ttgacctggc aaagcagaaa gcacaatcct caggcgggtt accgggtcaa aggtggtcgt
     8041 ccggcgctgg tggtggtgtt tctctgtggt attgctgtga ttggcgtgca atttttgatt
     8101 gcggcagggt tgttaccaga agtggggtga tcagatagcc tcaaattcct tattgggtgc
     8161 cagaattaac gctgacaccc aatgtgacct cttaatgcag gcagcactgc ttaaatttct
     8221 taccactgcc gcacggacaa ggatcgttac gccccggttt ctcttctgct ttgatcggtt
     8281 gctgaacagc tttttcctgc ggatgcgcca tccagtatgc atgtaaatca agcgccgcca
     8341 gtcgaatggc atctacgctc tcttcaaacg cttctggcga cattttctct acccgctcga
     8401 agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaaa gaatcgggca
     8461 acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac tcctcaacaa
     8521 tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac tgctccggga
     8581 attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc ataaagcgcg
     8641 tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac acggcaacca
     8701 gccactgttc tggttcaatc tcttgcggag aactcaacac cgccgtcaat aaaccgtcca
     8761 gctccgccac atcaaggatg gcgtggtcag tgttgtattt ggtcagaata tcgtccagcc
     8821 attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg tggatctcaa
     8881 ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga gtggctttgc
     8941 cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa aaccacccga
     9001 aggtggtttc acgacactgc ttattacttt gattttattc ttatctttcc catggtaccc
     9061 ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc cttgtgtcta
     9121 ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt cgaaccggac
     9181 tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt cttcaggcct
     9241 ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa acgagactcg
     9301 aactcgagac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcattca
     9361 tcaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc cgccgttcga
     9421 tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc acttttgatc
     9481 gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg cgcagcgctc
     9541 aaatattgca acattgacca cagagtcagt accgcagcca caaagaaaag tgcaataccg
     9601 gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac catctgggca
     9661 gtggttttca ctttcccaat ccaggagacg gccacgctac tgcgtttacc caactccgcc
     9721 atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc cgccggtaat
     9781 gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc cacgagaact
     9841 ttatctgcca cagggtcaag gaaagcacca aaccgggtac tctggttcca gcggcgtgcc
     9901 agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc ggcggcaaac
     9961 ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag gatgacacgg
    10021 aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta tctgttgtca
    10081 gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag aagatctttt
    10141 ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg gcgttacgta
    10201 aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc ccgacgcctt
    10261 caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg tgcccgccaa
    10321 tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg ggtgaatctg
    10381 gcggcaaact aaatccctca ccttccggct caaagaacag cgtttccagt ccagccttac
    10441 gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat gagacatcca
    10501 gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg ataaggatca
    10561 catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc acctgattca
    10621 tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga tactccgcac
    10681 gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg cccatggtat
    10741 ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg ctggcaagcg
    10801 cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag gccgtcgccg
    10861 cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg gtttgaacat
    10921 taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta tcgctaagat
    10981 taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt aaatagaact
    11041 gacctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact ttcgggaaat
    11101 agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca caagccatgc
    11161 ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc ggtattggaa acaaactgtt
    11221 tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acatgccgct tcttcaaact
    11281 ccagattctg gctggcagtt tccatacggc taatgagttg cgtaagcacc tgatcatctt
    11341 tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct tcactcacca
    11401 gtccttcaac gcacggtccc agacagcgcc ctatctggta ttgcagacac ggacgcgagc
    11461 gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc agtagcgcca
    11521 gtgtttcacg tacggcatag ccattcggga acgggccgaa atattcacct ttggcatgct
    11581 ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag ataaaaggat
    11641 aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg atgtagttgt
    11701 gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt tacatcaatt tgctggatct
    11761 gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct gcggaaatag ctggaaagcc
    11821 gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca tcgtacatgc
    11881 gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca aactgatcac
    11941 tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca ggtgagtcag
    12001 ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc tgttcaccgt
    12061 tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct tagtgatcat
    12121 cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg ttttttctgg
    12181 ttcgatctgg cttaacgcca tttgttgagc gatatcagaa gcaatgtaac gctgcccaga
    12241 atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc tgaggtagcc
    12301 agcagcacct gcctgcatga ctttcgctgg taaagggttt tctgtatgga ccgtaagcat
    12361 gatgatttta acatcagctg tggaacgcgc gattttacgc gtcgcctcaa gaccgccaat
    12421 gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc accacttaac
    12481 ggcgtcttca ccgcacgatg cctctccgac gacttttata ccctttatat cttccagaat
    12541 gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt tgatcaaagg
    12601 aatatctcca gaaataggga taacgctact gatagttagt cattcgtata ttagcggttt
    12661 ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc gtttttgtca
    12721 attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga tcgtttcaca
    12781 gtaagcccta cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat gaaaatcagc
    12841 ccgctgaata tgtaacatta attaacccga acaatacggg caaaaaacat tcatgataaa
    12901 aatatttatt ggtcattata ttaacgtatt ttatagcact gccgatatca cgctcaaaaa
    12961 acaaccactg ctatttttag caaagcttat ggtatactcc gccgccttaa catttttcac
    13021 cgcaaatttt cattgcaaca tgacgaggaa aataaatgag tacgcctgat ttttctactg
    13081 ccgagaataa tcaagaactg gcaaatgaag tctcctgcct gaaagcgatg ctgacgctga
    13141 tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat gttaaagatg gaaaaacagc
    13201 ttgcgctgat cgaagacgaa acccaggctg cagtattttc caaaacggtt aagcaaatta
    13261 aacaagccta ccgtcagtaa tataaaaccg gctgagagcg tgcctttcag ccggttattg
    13321 acaaaatcat cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt
    13381 tttcgcgctt gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca
    13441 gagccatcag actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa
    13501 tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca
    13561 gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg
    13621 gctgtgcttc gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag
    13681 ggttcagcac aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact
    13741 cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac
    13801 ataacgaata gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct
    13861 cttctgcggc ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat
    13921 ccgtatcctg catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat
    13981 tcaaactatc acataaataa gatttatata taacttatat tattcaggca atgaattact
    14041 tttgcaatcc atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt
    14101 ttatcacgct tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct
    14161 tattattgca gcagaagctt ctcgaggaac tggcgggtgc gaggctgctg gggatcggca
    14221 aataacgctt ttgctgcccc ctgctcgact atccgcccct ggtccataaa gatcgcccgg
    14281 tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa tcaccatcgt gcgcttttcc
    14341 tgcgccagct ggcggatggt gttcaggact tcgcccacca gctctggatc cagcgccgac
    14401 gttggctcgt caaacaaaat cacctcagga cgcattgcca gtgcacgcgc aatcgcaaca
    14461 cgctgctgtt gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca
    14521 actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg
    14581 atcaccggcc cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc
    14641 tggaagacaa acccgacgtg ctgacgcaac tggcgaatca gcgatttttg ctgacttaat
    14701 gaacgtgcag tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc
    14761 agcagattta tgctacgtag caatgtggtt ttgccggaac cactcggacc gataattgcc
    14821 accacttcgc caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtggaat
    14881 tttttcacca ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa
    14941 gttgattctc aaaatggttc tgcaacgtcg ataacacagt cgccatgatc cagtagatga
    15001 gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct
    15061 gacggaacag ctccggcacc tggattgtcg cggccagcga cgtatctttt accagactga
    15121 taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac
    15181 gcatggtctg ccacggcgtc ataccaatac tggcagcggc ttcccactga cctttatcaa
    15241 tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc ggcagtgttc agcgacagac
    15301 caatcattgc tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga
    15361 tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat agagatataa aaacgcgcca
    15421 gccagcgcac gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagtagta
    15481 agccaaaaaa catgccgccg atactgagtt gcagggtata cccagcccct ttcaacagga
    15541 acggcaagga atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac
    15601 gtggggatga taggcgaaca gcgcaggcgc gccgccggta tgaataaaca gaatcggccc
    15661 ttcatctttg aagcgtttct gactgatacc gtcaatcagc cccgccatcg cttttccggt
    15721 atacacagga tcaagcagaa tgccttccag acgcgccagc aatttcactg cttccatgcc
    15781 ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca tcccagagta aaatttccgc
    15841 tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa
    15901 ttgatcggca acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg
    15961 ttccagccca acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga
    16021 cgaaatatta accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc
    16081 tagcgcgcca agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc
    16141 ttcgactcgc gtcgccagct cttccagttg ggcattggga tcggtcagtg cgtcgcacat
    16201 ttcaatctgg gtattgaaca gatccagcaa caaacgattg ccgttggtta aatagttttc
    16261 tgcggttgtg ccaataggat tttccagcag cgccacgcag tgcagaccga gtttcgccgc
    16321 gactgcggca gtctggcgca catggttaga ctggatcgcg ccggcagtaa tcagcgtatc
    16381 ggcaccttcg cgcagggcat ctgccgcgag aaattccagc ttacgtaatt tattgccgcc
    16441 cattgccatg ggggtgacgt catcccgttt gatgaaaatt tcccgtccaa gataatcaga
    16501 aaagcgcggc agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg
    16561 ggttaaatta tgcagtggca taacagcctc cgatgtgtgt tgttgtgatt ttcttattat
    16621 gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa gcgccctttt ttgtcattat
    16681 gctgattatt tggtcacatc agcaccaaac catttttcgg aaagagcttg cagagtgccg
    16741 tctttttgca tttccgcaat tgcatcattc accgctttca gcaggtcttc atttccttta
    16801 cgcagcgcca cgccagactc ctgacgggag aatgcttctc cggttactgc cagcgtatcg
    16861 ttggttttct tcaccagatc cagcgccgcc agacgatcaa cgaggatcgc atcgatacgc
    16921 cctacgcgca gatcctgata tttggtcggg tcatcatcat aggtacgcac atcgacaccc
    16981 tgaacattct gccgcagcca ctcttcatag ttggtgccca gaccgacacc cacttttttg
    17041 cctttcagat cagcagccgt tttaatggtg ccttcgttac cttttttcac cagcgcctga
    17101 ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt tcttgcgctc gtcagaaatg
    17161 gtgacctgat taatcaccac atcaatgcgt ttagagtcca gcgacgccag cataccgtcc
    17221 catttggtcg gttttagtga cgcctcaacg ccaagatgtt ttgccagctg ttgggcaaat
    17281 tccacttcaa aaccggttaa tttgccgtca tccccctgaa aactgaacgg cggataagtg
    17341 ccttccagcc ctaccagcag cgtgccgcgc tctttgactt tattaagcag accttcatct
    17401 gcaaaacttt taacgctcat gcccgcaacc agcgccacgg ccatcacacc catcaatgcc
    17461 tgacgtccca gatgtgctaa tttcatattc accccgaatg ttgttatgtc tgtttgcagt
    17521 gtagagccat tgtttgtaaa cacaaaaaca actccgctac atcttattct tatttaatat
    17581 atatcagaag caggcaggct ggaggatttc tgcacaagtc cggtacaggt ttggcgctga
    17641 tagtgttgat acttccgtaa tgcgatgcgg taattgttgg tgctggtggt gggcagccac
    17701 ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat attttgctca
    17761 tgtagatgat tacccaaacg gcgcagacga acgacatatt cacgcaccgt acctgggctc
    17821 atttcagttt gctcaaaaag aaatttctta aagccgataa tatcgaaaaa atcactctgc
    17881 gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg cccatgattg ttgttccgtt
    17941 tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt
    18001 ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc
    18061 tggctgtgtt taaagtcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc
    18121 atcattaaga actcctggta gtcaaagtta aagtgcggca tttactgacg ttataactta
    18181 cccagtttag tgcgtaaccg tttaatggcc tggctgtgta actgactgac ccgcgattcc
    18241 ccgacctcca gcaccgcgcc aatctctttg agattcagct cttcctggta atagagggtt
    18301 aataccagtt tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga
    18361 ttactgtcca gcagttgttg tagcgggttt tctcgctgat gatcatcagt aaccagttcg
    18421 atgctatcgc cgtgctcttc acgccactca tcgtaggaga agagctggct gttattggtg
    18481 tcgagcagca tttgacgata atcggcaata tcgatcccta aacgttccgc tacctcagtt
    18541 tccgtggcgt tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc
    18601 tcgttgcgtc gcacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg
    18661 ccacggatac gctgcactgc gtaagttgta aatgccgttc cttgtagggc gtcatagcgt
    18721 tcgacagcat taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc
    18781 gcgggcagtc gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc
    18841 cacagcgagt gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc
    18901 cctgcgttat atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga
    18961 ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa aagccgaatg taaaaaaccc
    19021 cgccggtggc ggggtttgag aggcattcag acgattaacc ctgcagcaga gacagaacct
    19081 gctgcggtac ctggttggct tttgccagca cggagttacc ggcctgctgg ataatctgcg
    19141 ctttcgacat gttggacact tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt
    19201 cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc ggaatccaaa cggttttgca
    19261 ccgcacccag ggaggagcgg aatttgtcga tggagctgat ggcttcatcc agagctttca
    19321 ggggatcggc tgtcgtagct gcttttgttt cagcatctgt cgtcaattta ccatcagcat
    19381 ctttataaac tgccttaccg ctgccgttag tcactgaacc atcatcctgt aggtaaaaat
    19441 ctacagtatc agatgtggtc aacgccccgt tgttatcaac ataaaccgaa tcaacacctt
    19501 ttgttacagc gccagttgtg ccgttgacgc tataacctgc agtagtgaaa tcaacattgt
    19561 ttcccgaggt tgatacagct ttatcgaaag ctgcctaatc aacttttgca cctgttactg
    19621 aataagtaat agtgtccggc gtagtactat taccggtaaa ggagatagag gtaccgccaa
    19681 tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt ggacagacta gcctcagtca
    19741 aagaagcacc agagccgttt ttcgttaagc gcccagttgt atcaatgtag agtttatctc
    19801 cattgcttgc cgtaatttta ccatcactgc tgatctgaac atcctgtgca gaaccatcga
    19861 tagtaatagt gcccttagcg gtatcaccaa ctcccggggt caaatatttc tgaacatcgg
    19921 cagctgaagc cgttgtggta tcaaaagagt atgacttact tgcactgtca tacttgtaat
    19981 tcgttgcact agccgccgtt ccgaagccgt tattaatggt tgcctgaaca gtatcaccat
    20041 cactaactcc agccagcaaa tcagacgctt tagcagcatc gtaacccgca ctcacagtat
    20101 atttgttgcc gaccactgtt gcattagcag ctaccaagtc agctttagat gcagcagtat
    20161 tagccacagc cccgccacca ttcacattaa acccattcag ccccagcgta tcagagtcaa
    20221 ttttcttcag atcaatagtg atagtctggc cgtcattcgc accaacctga attttcatcg
    20281 aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt ctgaccggat acgcggtcaa
    20341 tttcgtcgag acgggatttg atttcgtcct gaatggagtc cagatccgaa tcagagttag
    20401 tcccggtaga agcctgaacc gtcagctcac ggatacgctg taagttgttg ttgatttcgg
    20461 acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc gttggcgtta cgtgcagcct
    20521 gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat cgcctgaccc gcggcgtcat
    20581 ccttcgcgct gttaatacgc aagccagaag acagacgctc gatagaactc gacagcgcag
    20641 actggttctt gttgatatta ttttgagtga tcagcgagag gctgttggta ttaatgactt
    20701 gtgccatgat tcgttatcct atattgcaag tcgttgatta cgttttgggt ttccacccgt
    20761 cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa acaaccttta gaattttttt
    20821 cacaaacagc cattttttgt tagtcgac
//
LOCUS       DADLSE010000064        20681 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLSE010000064 DADLSE010000000
VERSION     DADLSE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.064"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..66)
                     /locus_tag="JN692_002323"
     CDS             complement(<1..66)
                     /locus_tag="JN692_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7264619.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMR"
     gene            74..574
                     /gene="tnpA"
                     /locus_tag="JN692_002324"
     CDS             74..574
                     /gene="tnpA"
                     /locus_tag="JN692_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7264620.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(613..960)
                     /gene="grxD"
                     /locus_tag="JN692_002325"
     CDS             complement(613..960)
                     /gene="grxD"
                     /locus_tag="JN692_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7264621.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1295..2122
                     /locus_tag="JN692_002326"
     CDS             1295..2122
                     /locus_tag="JN692_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7264622.1"
                     /translation="MARINRISITLCALLFTTLHLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEIYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2250..2831
                     /gene="sodB"
                     /locus_tag="JN692_002327"
     CDS             2250..2831
                     /gene="sodB"
                     /locus_tag="JN692_002327"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7264623.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2977..4146)
                     /locus_tag="JN692_002328"
     CDS             complement(2977..4146)
                     /locus_tag="JN692_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264624.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGSPLMTLLLSHRARRSALIFLMAIFTPGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFLGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4312..4401)
                     /gene="ynhF"
                     /locus_tag="JN692_002329"
     CDS             complement(4312..4401)
                     /gene="ynhF"
                     /locus_tag="JN692_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7264625.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4700..5725
                     /gene="purR"
                     /locus_tag="JN692_002330"
     CDS             4700..5725
                     /gene="purR"
                     /locus_tag="JN692_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7264626.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5722..6654)
                     /locus_tag="JN692_002331"
     CDS             complement(5722..6654)
                     /locus_tag="JN692_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7264627.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6767..7978
                     /locus_tag="JN692_002332"
     CDS             6767..7978
                     /locus_tag="JN692_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7264628.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8269..9417
                     /gene="cfa"
                     /locus_tag="JN692_002333"
     CDS             8269..9417
                     /gene="cfa"
                     /locus_tag="JN692_002333"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7264629.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9457..10098)
                     /gene="ribE"
                     /locus_tag="JN692_002334"
     CDS             complement(9457..10098)
                     /gene="ribE"
                     /locus_tag="JN692_002334"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7264630.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10313..11686
                     /gene="mdtK"
                     /locus_tag="JN692_002335"
     CDS             10313..11686
                     /gene="mdtK"
                     /locus_tag="JN692_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7264631.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTATRTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11727..12983)
                     /locus_tag="JN692_002336"
     CDS             complement(11727..12983)
                     /locus_tag="JN692_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264632.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13291..13367
                     /locus_tag="JN692_002337"
     tRNA            13291..13367
                     /locus_tag="JN692_002337"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13325..13327,aa:Val,seq:gac)
     gene            13372..13448
                     /locus_tag="JN692_002338"
     tRNA            13372..13448
                     /locus_tag="JN692_002338"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13406..13408,aa:Val,seq:gac)
     gene            13556..13861
                     /locus_tag="JN692_002339"
     CDS             13556..13861
                     /locus_tag="JN692_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7264633.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13987..15522
                     /locus_tag="JN692_002340"
     CDS             13987..15522
                     /locus_tag="JN692_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7264634.1"
                     /translation="MKKIAIVGAGPTGIYTLFSMLANIASIEIPPINCTYLEWLQKQE
                     ASHLQRYGVKKETLHDRQFLPRILLGEYFRDQFLRLVDQARQQKFAVAVYESCQVTDL
                     QITNAGVMLATNQDLPSETFDLAVIATGHVWPDEEEATRTYFPSPWSGLIEAKVDACN
                     VGIMGTSLSGLDAAMAVAVAVAVAVAVAIQHGSFIEDDKQYVVFHRDNASEKLNITLM
                     SRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKGEEGLLDRVFRLIVEEIKFADPDW
                     SQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNLQEVERNKREKHTVPWRYVILRLH
                     EAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPSESIRRLLALREAGIIHILALGED
                     YEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKVKDIPFPGLREQLQKTGDEIPDVG
                     EDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTACAEIGEAMARAVVKPASRARRRL
                     SFD"
     gene            complement(15534..16346)
                     /locus_tag="JN692_002341"
     CDS             complement(15534..16346)
                     /locus_tag="JN692_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264635.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16350..17135)
                     /locus_tag="JN692_002342"
     CDS             complement(16350..17135)
                     /locus_tag="JN692_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HAY7264636.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17132..17800)
                     /locus_tag="JN692_002343"
     CDS             complement(17132..17800)
                     /locus_tag="JN692_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7264637.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAYIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17864..18502)
                     /locus_tag="JN692_002344"
     CDS             complement(17864..18502)
                     /locus_tag="JN692_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HAY7264638.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18515..20617)
                     /locus_tag="JN692_002345"
     CDS             complement(18515..20617)
                     /locus_tag="JN692_002345"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HAY7264639.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPLGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLIPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGKEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKPMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
BASE COUNT         5134 a         5375 c         4854 g         5318 t
ORIGIN      
        1 cctcatttca cgctcctgtt gaccaccggg tcttaactgg aatttaaatg cctgtaatcg
       61 tttcacaggt caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag
      121 gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accaaatacc gacgccagat
      181 ttttgaccat gacacgacag aaaaactacg cacttacttt tcaaatgtat gtgctgattt
      241 tgcagctgaa ctggttgaaa tggatggcga accagatcac gtccatttgt taattaacta
      301 tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcatat cgaataggtt
      361 actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag
      421 tcctggctat tttgccagta gctgcgaagg tgcgccaata tccgtcatcc gccaatacat
      481 tgaacaacag caaacacctg gtcaggtgga aaaccgcgcc ttatatcccc gccctgaagg
      541 acgaggtttt acggcgcacc ggataaagga ctgacggatg cccgttcgca tccgtcagta
      601 ttgcaggacg gattattccg cgtccggctc ttcagacttg tatttagcgg cagtttcttt
      661 gatcagctgc tgcagttcgc cacgctgata catttcgatc acgatatcac aaccgccgac
      721 cagctcgccg tcaacccaca gttgcgggaa ggtcggccag ttagcatatt tcggcagttc
      781 cgcacgaatg tccggattct gcagaatatc aacataggca aaacgttcgc cacatgcggc
      841 aagcgcctgg actgcctggg cagagaaacc gcagctcggc agtttcggtg aacctttcat
      901 gtacagcagg atcgggtttt cagcaatctg gcgttggatt ttttcgatag tggtgctcat
      961 tgtcttgctt cctcaaactt ctttacggca gtaatctgac attgtagcgg gtcagtgcgg
     1021 catcggaaaa taacattttc atcacgcttt tgctatttta tccctttgct ctatttgttg
     1081 catttcaaat attcgtttct tctctgatgc gcggcaaact ggcgtattta taaccatttt
     1141 tagctttcac tgctgcattt ttttgcgctc gccaacgaaa cgtatttttt aacaataaaa
     1201 gctattaact ttctcttctt ctatgcatta gaatcatcaa gttttgtaaa tcagacgcag
     1261 gcatgataga cctgccttaa cagagggacg ctcagtggcg cggataaacc gtatttcgat
     1321 cacgctctgt gctttacttt ttaccaccct gcatttaacg cctatggccc atgcttcaaa
     1381 gcaagccagg gagagttctg ctaccactca tatcaccaaa aaagcagata aaaagaaaag
     1441 cacggcaacc accaaaaaga cccagaaagc cagccagaaa acagcgaaaa aagccgccag
     1501 taaaagtacg accaaaagca aaaccgcttc ttccgttaaa aaatcttcca ttaccgcttc
     1561 taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa acggcctcag ccagctttac
     1621 cgaaaagtgt accaaacgta agggctataa atcgcattgt gtgaaagtca aaaatgccgc
     1681 gtcaggaacc cttgccgacg cgcacaaagc gaaggtgcaa aaagccacga aagtggcaat
     1741 gaataaactg atgcagcaaa ttggtaagcc atatcgttgg ggcggcagct caccgcgtac
     1801 cggttttgat tgcagcggcc tggtttatta cgcttataaa gatttggtga aaattcgtat
     1861 tccgcgtacg gcgaatgaaa tatatcacct gcgtgatgca ggacctatcg aacgtagtga
     1921 gctgaaaaac ggcgacctgg tctttttccg tactcaggga cgcggtacag ccgatcatgt
     1981 cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg cgcacaggtc aggaaattca
     2041 aatcacttct ctcagtgaag actactggca gcgccactat gttggcgctc gtcgggtaat
     2101 gaccccaaaa acacttcgct aaaactttac cctgttgtta cggcaacagg gtaagttcat
     2161 cttttgtctc accttttaat ttgctaccct atccatacgc acaataaggc tattgtacgt
     2221 atgcaaatta ataataaagg agagtagcaa tgtcattcga attacctgca ctaccatatg
     2281 ctaaagatgc tctggcaccg cacatttctg cggaaactat cgagtatcac tacggcaagc
     2341 atcatcagac ttatgtcact aacctgaaca acctgattaa aggtaccgcg tttgaaggta
     2401 aatcactgga agagattatt cgcagctctg aaggtggcgt attcaacaac gcagctcagg
     2461 tctggaacca tactttctac tggaactgcc tggcaccgaa cgccggtggc gaaccgactg
     2521 gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt tgccgatttc aaagcgcagt
     2581 ttactgatgc agcgatcaaa aactttggtt ctggctggac ctggctggtg aaaaacagcg
     2641 atggcaaact ggctatcgtt tcaacctcta acgcgggtac tccgctgacc accgatgcga
     2701 ctccgctgct gaccgttgat gtctgggaac acgcttatta catcgactat cgcaatgcac
     2761 gtcctggcta tctggagcac ttctgggcgc tggtgaactg ggaattcgta gcgaaaaatc
     2821 tcgctgcata ataactgatg gcaaatgcag cattgcctga tgcgcttcgc ttaccatgcc
     2881 aacgcggatg aggcgttaac agcgcattcg gcaagataaa tcgcacgttg tcagcaactg
     2941 taacgcagaa ggttatcctt ctgcgttttt gtttaattag ctgttagcaa cgcaaactgt
     3001 ttcaggttgt tttctggctg acataaacac cagcaataat gccagtcccg cgacaatcgc
     3061 ccccgtcacc ggcacaaagc tgtatcccag cccagcggaa attaccgcac caccagcagc
     3121 tgctcccagc gcatttccaa gattaaaggc accaatattg actgatgaag acagacctgg
     3181 cgcttcactg gcgacatgca tcacgcgcat ctgtaacgga ggtacaaccg caaaggttgc
     3241 tgcgccccac accaccatgc taatagctgc gccgaactca ttgcgggcca ggaacgggat
     3301 tgccagcata atcaccatca gcagcaacaa aaagcctttc aacgtgccgt taactgaacg
     3361 atctgccagt ttgccgccga gatagttacc gatagagaat ccgacaccaa tcagcaccag
     3421 cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt tgcagtaccg gagagatata
     3481 ggtgtagagg gtaaacattg caccagctcc cagtaccgtc gtcagcaatg cagacagcac
     3541 ctgcggacgc attaataccg ccagctcttt tttcacttca ggtcgtgccc ctgtaccacc
     3601 tttaggtaat gagaagaaca gacttaccat tgaaatcact cccagccccg ccgttgccag
     3661 aaatgacatc cgccagccga tggtttcacc caaccaggtc gccgccggca cgccaccgat
     3721 atttgccagg gttaacccca taaacatagt ggcaactgcg ctggcctgtt tatgttttgg
     3781 caccacgctt gcggccacga ctgaacccaa acccaaaaat gctccgtgat tcaggctggt
     3841 caaaatacgt gaaagcatca gggtcatata atccggcgcg atggcggaaa gaacgttgcc
     3901 tggcgtgaaa attgccatca ggaaaatcaa cgcactgcgg cgggcacgat gagaaagtag
     3961 aagcgtcatc agcggcgagc ccaccattac gccaactgca taggcactga ttaacattcc
     4021 ggcagcggga atcgagacat ccacaccgcg cgcaatgacg ggcaacaagc ccattggcga
     4081 gaactccgtt gtcccgatac caaacgcgcc aatcgccagc gccagcaacg gatagttaat
     4141 tttcatgcct tatctccacc tcttcgcgtc attacgcgat attcattaaa gtggcgaaag
     4201 catgacagca atcacaaaaa aatgaaaata acaaaaagag aaaacacttt tgccattttg
     4261 ctaacaaaca ggaaggagat gcgagggaga acgcgctccc tcgagaggaa atcagtgcag
     4321 cgcggcagtc aaacccacgg ctacgatcaa accgaggacg ataatcgttg ttaccagtga
     4381 aaatttaagg tcggtgctca tcaagttttc tcctttttta ttaccacaca aaaagtgata
     4441 ttacgcattt ttacacactg tgatgaaaaa atctcccgtc atttataatg ataagtgttt
     4501 ttaccacttc cccttttcgt caagatcggc caaaattcca cgcttacact atttgcgtac
     4561 tggccattga ccccttcctg acgctccgtg tcgtttttcc ggcgtaccgc aacacttttg
     4621 ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga ttttgcagga gctgaagtta
     4681 gggtctggag tgaaatggaa tggcaacaat aaaagatgta gcgaaacgag caaacgtttc
     4741 cactacaact gtgtcacacg tgatcaacaa aacacgtttc gtcgctgaag aaacgcgcaa
     4801 cgccgtgtgg gcagcgatta aagaattaca ctactcccct agcgcggtgg cgcgtagcct
     4861 gaaggttaac cacaccaagt ctatcggttt gttggcgacc agcagcgaag cggcctattt
     4921 tgccgagatc attgaagcag ttgaaaaaaa ttgcttccag aaaggttaca ccctgattct
     4981 gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat ctgtcgatga tggcgcaaaa
     5041 acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca gagccgttgc tggcgatgct
     5101 ggaagagtat cgccatatcc caatggtggt gatggactgg ggtgaagcaa aagctgactt
     5161 caccgatgcg gtcattgata acgcgttcga aggcggctac atggccgggc gttatctgat
     5221 tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg ctggaacgta acaccggcgc
     5281 aggccgcctt gccggtttta tgaaggcgat ggaagaagcg atgatcaagg tgccggaaag
     5341 ctggattgtg cagggtgact ttgaacctga atccggttat cgcgccatgc agcaaatcct
     5401 gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc gatatcatgg caatgggcgc
     5461 actttgtgct gctgatgaaa tgggcctgcg cgtcccgcag gatgtttcgc tgatcggtta
     5521 tgataacgtg cgcaacgcgc gctattttac gccggcgctg accacgatcc atcagccaaa
     5581 agattcgctg ggtgaaacag cgttcaacat gctgttggat cgtatcgtca acaaacgtga
     5641 agaaccgcag tccattgaag tgcatccgcg tttgattgaa cgccgctccg tggctgacgg
     5701 cccgttccgc gactatcgtc gttaatcacc cgttgcggga gtctcttccg gctcccgcag
     5761 ccactcctta ttcagcgtct cactatcgcc gagatactca agcagccagg ttaacgcagg
     5821 cgacatatca ttttgctgcc atgtcagaca acatgccgaa tccgggaagg ggttttccag
     5881 ttcaagtgct acccacttcc cctcattaag ccacggtttg gcgaaatgtg ttggcaccat
     5941 ccctatgcat aatcctgccg agatacaggt tgccgatgat tcccagtcag gtacgacaag
     6001 tcttttttgg ttatccagca accaggtaat acgtttaggt agcgttcgcg aggtgtcttc
     6061 gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg aacgggccat ccatcaacgc
     6121 cagcgggtgg tgactggcaa caacgcaact ccagcttagc atccccatat cccggaaggc
     6181 ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc gccagttcca cgcgcccgtc
     6241 ggaaagcgca tcccagacac cgttgaacac ttcctgaaag acaagaagtt cgacatcatc
     6301 aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt tctggcctga caatattatc
     6361 cactgcgata gctaactgac cgcgccagcc gttcgctatc tgctgacatt gctggcgggt
     6421 gatctgcatt tttttgacaa cagagcgccc ttctttgaga aaccacgctc cagcagcggt
     6481 cagctccaca tcacggtgcc gtcgttcaaa gagcggtacc gccagccact cttccagctg
     6541 acgcacggta tagctgaccg cagaaggaac gcgatgcagc tcctgtgccg cagcgctaaa
     6601 actaccatta cgcgctaccg catcaacaac ttcgagtgaa tattctgacc acatagtctg
     6661 cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt tcacaacact aatttcactc
     6721 cctacacttt gcggcggtgt ttaattgaga gatttagaga atatacatgc aacctgggaa
     6781 aagattttta gtctggctgg cgggtttgag cgtactcggt tttctggcaa ccgatatgta
     6841 tctgcctgct ttcgccgcca tacaggccga cctgcaaacg cctgcgtctg ctgtcagtgc
     6901 cagccttagt ctgttccttg ccggttttgc cgcagcccag cttctgtggg ggccactctc
     6961 cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg acaatttttg cgttaggtag
     7021 tctggggatg ctgtgggtag aaaacgccgc cacgctgctg gtattgcgtt ttgtacaggc
     7081 tgtgggggtc tgcgccgcgg cggttatctg gcaagcatta gtgacagatt attatccttc
     7141 acagaaagtc aaccgtattt ttgcgaccat catgccgctg gtgggtctgt ctccggcatt
     7201 ggctcccctg ttaggaagct ggctgctggt ccatttttcc tggcaggcga ttttcgccac
     7261 cctgtttgcc attaccgtgg tgctgattct gcctattttc tggctcaaac ccacgacgaa
     7321 ggcccgtaac aatagtcagg atggtctgac ctttaccgac ctgctacgtt ctaaaaccta
     7381 tcgcggcaac gtgctgatat acgcagcctg ttcagccagt ttttttgcat ggctgaccgg
     7441 ttcaccgttc atccttagtg aaatgggcta cagcccggca gttattggtt taagttatgt
     7501 cccgcaaact atcgcgtttc tgattggtgg ttatggctgt cgcgccgcac tgcagaaatg
     7561 gcaaggcaag cagttattac cgtggttgct ggtgctgttt gctgtcagcg tcattgcgac
     7621 ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa atcctgatcc cattctgtgt
     7681 gatggcgatt gccaatggcg cgatctaccc tattgttgtc gcccaggcgc tgcgtccctt
     7741 cccacatgca accggtcgcg ccgcagcgtt gcagaacact ctgcaactgg gtctgtgctt
     7801 cctcgcaagt ctggtagttt cctggcttat tagtatcagc acgccattgc tcaccaccac
     7861 cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt tacatgatgc aacgttgtga
     7921 agaagttgac tgcccgaatc atggcaatgc cgaagtcgct catagcgaat cacactgatc
     7981 tatatcgata tacttatact taggctgcta acaaaatttt gttgtatgat tgaaattagc
     8041 ggcctatact aatttcgagt tgttaaagct acgataaata ttatgttttt acggggacag
     8101 gatcgttccc gactcactat ggatggtcat ttcggcaagg gttcctcctt tccctctgtt
     8161 ctacgtcgga ttatagactc gcggtttttt ctgcgagatt tctcacaaag cccaaaaagc
     8221 gtctacgctg ttttaaggtt ctgatcaccg accagtgatg gagaaactat gagttcatcg
     8281 tgtatagaag aagtcagtgt accggatgac aactggtacc gtatcgccaa cgaattactt
     8341 agccgtgccg gtatagccat taacggttct gccccggcgg atattcgtgt gaaaaacccc
     8401 gattttttta aacgcgttct gcaggaaggc tctttggggt taggcgaaag ttatatggat
     8461 ggctggtggg aatgtgaccg actggatatg ttttttagca aagtcttacg cgcaggtctc
     8521 gagaaccaac tcccccatca tttcaaagac acgctgcgta ttgccggcgc tcgtctcttc
     8581 aatctgcaga gtaaaaaacg tgcctggata gtcggcaagg agcattacga tttgggtaat
     8641 gacttgttca gccgcatgct tgatcccttc atgcaatatt cctgcgctta ctggaaagat
     8701 gccgataatc tggaatctgc ccagcaggcg aagctcaaaa tgatttgtga aaaattgcag
     8761 ttaaaaccag ggatgcgcgt actggatatt ggctgcggct ggggcggact ggcgcactac
     8821 atggcatcta actatgacgt aagcgtggtc ggcgtcacca tttctgccga acagcaaaaa
     8881 atggctcagg aacgctgtga aggcctggat gtcaccattt tgctgcaaga ttatcgtgac
     8941 ctgaacgacc agtttgatcg tattgtttct gtggggatgt tcgagcacgt cggaccgaaa
     9001 aattacgata cctattttgc ggtggtggat cgtaatttga aaccggaagg tatattcctg
     9061 ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg atccctggat taataaatat
     9121 atttttccga acggttgcct gccctctgta cgccagattg ctcaatccag cgaaccccac
     9181 tttgtgatgg aagactggca taacttcggt gctgattacg atactacgtt gatggcgtgg
     9241 tatgaacgat tcctcgccgc atggccagaa attgcggata actacagtga acgctttaaa
     9301 cggatgttta cctattatct gaatgcctgc gcaggtgctt tccgcgcccg tgatattcag
     9361 ctctggcagg tggtgttctc acgcggtgtt gaaaacggcc ttcgagtcgc tcgctaaagg
     9421 ctattctatc gccccctctt cgggggcgag ttcagatcag gcttctgtac ctggttgatt
     9481 catggcattt tctcgtgccg ccagcacacg ttctaccgta tctaccactg cctgagtttg
     9541 tggatcgatt tcaatgttga cgcgtgcgcc aagttttttc ttcccaagag tcgtgcgttc
     9601 cagtgtttcc ggaattaaat ggacgcaaaa acgcgttggc gtgacttcgc cgacggtcag
     9661 gctaataccg tcgatgccaa taaatccttt gtacaggata tatttcatca actgactatc
     9721 ctggacttta aaccagatct ggcgattatt ttccgaggtt aatattttcg ccacttcagc
     9781 ggtggtcata atatgacctg acattaagtg tccgccaatt tcatcactga atttcgccgc
     9841 acgctcaacg tttacccaat cccccacttt taaatcgcca agattggtaa tgcgtaacgt
     9901 ttctttcatc aggtcaaaac tgacatggtt gccgttaatt tccgtcacgg tcaggcagca
     9961 accgttatgc gccacggaag caccggtttc caggccgtcc agcatgtggt cgggtaactc
    10021 caccacatgc gtacgaaaat ttggtttctc gtcaatcgac accagttttg cggtgccctg
    10081 tacaatcccc gtaaacatac ttacaactcc tgaaatcagt taagacattc tgttcagcac
    10141 aatagcaggt ggaaaacgcc cttaccagtg aaggggtaag aatggctatt ttttcactgg
    10201 agaattaata aatcctcgct acaatagact gaatttcccc tgcttcttct ttttgctgcc
    10261 cattcaggcg gctttttagt ctctcatata actacaaata aaaggtgttc acgtgcagaa
    10321 gtatatcagt gaagcgcgtc tgttattagc attagcaatc ccggtgattc tcgcgcaaat
    10381 cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg ggcggctata gtgccaccga
    10441 catggcggcg gtcgctatcg gtacttctat ctggcttccg gcgatcctct ttggtcacgg
    10501 actgctgctg gcattaacgc cggttatcgc gcaattaaat ggttccggtc gacgtgagcg
    10561 cattgcgcat caggtgcgac aaggtttctg gctggcaggt tttgtttccg ttctcattat
    10621 gctggtgctg tggaatgcag gttacattat ccgctccatg gaaaacatcg atccggctct
    10681 ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg ggcgcgccgg gatatctgtt
    10741 cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa accaagccgg gtatggtaat
    10801 gggctttatc ggcctgctgg tgaacatccc ggtgaactat atctttattt atggtcattt
    10861 cggtatgcct gagctcggtg gcgttggttg tggcgtggct actgcggcgg tgtattgggt
    10921 catgttcctt gccatggttt cttacattaa acgcgcccgc tccatgcgcg atattcgtaa
    10981 cgaaaaaggc accgctaagc ccgatcctgc ggttatgaaa cgactgattc aactcggttt
    11041 gccgattgcg ctggcactgt tctttgaagt gacactgttt gccgtcgtgg ctctgttagt
    11101 gtctccgctc ggtattgttg atgtcgcagg acaccagatt gccctgaact ttagttcact
    11161 gatgtttgtg cttccaatgt cgctggcggc agcggtaact atccgcgtag gttatcgtct
    11221 gggtcagggc tcaacgctgg atgcgcaaac cgctacgcgg accgggctta tggtgggtgt
    11281 ctgtatggca accctgacgg ccattttcac ggtttcactg cgggagcaaa tcgccctgtt
    11341 gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat ttgatgttgc tggcggcggt
    11401 atatcagatt tctgactcaa tccaggtgat tggcagtggg attttgcgtg gttataaaga
    11461 tacgcgttcc attttctata tcacctttac agcttactgg gtgctgggct tgccaagcgg
    11521 ctatattctg gcactgaccg atctggtcgt tgaacctatg gggccagcag gcttctggat
    11581 aggctttatt atcggcctga catcggcagc cattatgatg atgctgcgta tgcggttcct
    11641 gcaacgtatg ccgtcagcca tcattctgca acgagcgtcc cgctaataaa gacaaggcgc
    11701 aaccttcacg ggttgcgcct gtgtttttac gcaggctgga gcgttgcgcc aatcccgtct
    11761 tcgtctggct gtaatttcag agcgttacac agagtttgcc gactggcatc tgttatctca
    11821 acgcgtatat ccccgttaac ttcccctttc acattgatga agtcaggtga aatttctttc
    11881 gcactggttt gcaggcaaat cgctccctct ccgcgcaggc gtttcacttt cagttgcaca
    11941 tgcgttgccg caccagtgat cagccacagt tttccgccat tcagattgat gttaccaaca
    12001 atgaaagtga cgctgtcacc ttttaccgta ctgccgtcga gaatcaacac accgccctgc
    12061 tgtacagtaa tcgttgattg ttgcagctct gcgccataac caataatcat tgaagcgccg
    12121 tccagtaatg caacactccc ggtcagttca accggtgcgc gatcttcctc tacgacaaaa
    12181 ttttcgtccg cgctggtggg tgacaacagg atcactgttc caccttgtat taccgcattt
    12241 tccagcgtac cggcatgcac ttcagcccga ccccccacat tcaccgacag gttttgcgtc
    12301 ttaccggaac tgtagagctg aaggccatcc gtccccagac gataaatgga tccattctca
    12361 acaatagtgt cagagtcttc accaccgaga ttattcatgg tgccaccagc aagcaacgta
    12421 tcactggctt tcccggccag cataaaaacg ccgccctgat taatctcaca attgctggct
    12481 tctccacttg gatacaccaa caattcacca ccttcatcta ccacgaccgc tgaggttgtc
    12541 ccattaacca acaggccgcc ctcttgatcg agaatgattt tttccgcgcg atgtccttcc
    12601 agtacacgca ggttaccgcc attttccagc aacaaaccgc aggcataacc ctgatcaacg
    12661 ctgaattcac cttcgggatg gcggccatta accgtagcga gcgtagagag actaattgcg
    12721 cctccctcgg actgaacaat atctgtggca atggcaccgt cgctgaccga tagcgtgcca
    12781 ttgttaccgt actgtgtgca tttggcgatc ccaccggttg ccactttcaa ccagccagcc
    12841 cccgtgacca cggtattttc ggctcttccg ccagcttcaa caatcaactc gccttcagta
    12901 agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt gcacattccc gtcgctcatc
    12961 aagtttttcg catcagatcc catgatttat tcctttgctg catctgtgtg cctttattgc
    13021 tacctaagtg taaaggctac ggaggattta tccacgacag atttgagatg gtggcaaaca
    13081 actctgttta aactctgata cacgaattat tgggttgtat cagatgtaaa tgcgatcctg
    13141 aataaaaatc gcccttgcaa atcaacaaaa tatagccaat ttgcttaaaa gctctgcaaa
    13201 cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc acctttccac tctgccgcta
    13261 atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag ctcagttggt tagagcacca
    13321 ccttgacatg gtgggggtcg ttggttcgag tccaattgaa cgcaccatcc tgcgtccgta
    13381 gctcagttgg ttagagcacc accttgacat ggtgggggtc ggtggttcga gtccactcgg
    13441 acgcaccaga ttttcttaat ctggtctttt cctttttccc tctgtttctt ctctgtatcc
    13501 aatacgttaa aagatttaca ctgtcttcgt atgcgttatc agaaggagaa tcgctatggc
    13561 aactttgtta caacttcatt ttgcttttaa tggcccattt ggcgacgcaa tggctgagca
    13621 gcttaaacca cttgctgagt cgattaatca ggaacctggt tttctgtgga aggtatggac
    13681 agaaagtgaa aagaaccacg aagccggtgg gatctacctt ttcactgatg aaaaaagcgc
    13741 ccttgcctat ctggaaaaac atactgcccg actgaaaaat ctcggcgttg aggaagttgt
    13801 cgccaaagtt ttcgatgtca atgagccact tagtcaaatc aatcaggcaa aactcgcctg
    13861 acagaattta atcaagggcg gttagcgccc ttttcatccc tgtctgaaat ttctcaaatt
    13921 ctaaaaatct caaccaaact tatctgataa cactaaattc gaaagaatgc gtacaggtaa
    13981 gtaacaatga aaaaaattgc tattgtgggt gccgggccta cggggatcta caccttattc
    14041 tcgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa
    14101 tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg
    14161 cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta
    14221 cggttagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag
    14281 gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc
    14341 agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa
    14401 gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga tagaagcaaa ggtcgatgcg
    14461 tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggca
    14521 gtggcagtgg cagtggcagt ggcagtggct attcagcatg gttcgttcat tgaagatgat
    14581 aaacaatacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
    14641 tcgcgcacgg ggattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta
    14701 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
    14761 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
    14821 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    14881 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    14941 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    15001 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
    15061 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta
    15121 cgtgaagcgg ggatcattca tattctcgcc ctcggtgaag actacgaaat ggaaattaat
    15181 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat
    15241 gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa
    15301 ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc
    15361 gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggttgatgca cgaccagcct
    15421 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
    15481 aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
    15541 tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca
    15601 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt
    15661 ccccctggcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
    15721 ccgctagcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag
    15781 ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa
    15841 acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc
    15901 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
    15961 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
    16021 gcggttgcga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
    16081 gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa
    16141 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
    16201 ctctccgccc agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta
    16261 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
    16321 cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
    16381 ttaaaggttt cgtgtggcgt acgtcccgtg gtgcaaagat aaagatgacc gaagataaaa
    16441 aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga
    16501 aacacatctc ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc
    16561 agcaatccat acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt
    16621 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
    16681 tgttttgccg ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc
    16741 ccaacggcat tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact
    16801 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc
    16861 agcccacttg ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc
    16921 gctttgctgt aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga
    16981 gcacgaagca aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca
    17041 atgatcaacc acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg
    17101 ctctgaaact gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt
    17161 gaccgaaccg gcgatacaga tgcggttttc cggcaccagg tagctgatat tgatagtatt
    17221 tattatcctg caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt
    17281 gttccggaca ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc
    17341 agaaatcaca tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac
    17401 aagcgccaat gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct
    17461 gctcatcacg ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt
    17521 gctgacatga ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa
    17581 tgtatgctat cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc
    17641 gggcgcaaat attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt
    17701 ccttttcttg cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg
    17761 ttcccatccc cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag
    17821 tggctgcaac tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact
    17881 gctctacctc gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc
    17941 taaacaagta caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg
    18001 caacatccat cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt
    18061 gcatcagcat ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt
    18121 gtgcaatggc gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt
    18181 cactcatggc ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat
    18241 ccgcgtagtc tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa
    18301 ccgaataggg tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct
    18361 ggcgaatgaa ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt
    18421 gacgcatccc ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg
    18481 gcaattcatc ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg
    18541 ccagatcggc ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac
    18601 caagctgtgg gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt
    18661 cacgatccat tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa
    18721 cccaggaaca gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg
    18781 tgtaggcacg atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca
    18841 tctcttcgcc ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat
    18901 tacggctttt cagcggcgaa acggtcattg gccagaccca gttgcacagg gtgacggcgt
    18961 tatgcaaaca gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg
    19021 gcgtgtagtt tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt
    19081 cacgttgcag tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc
    19141 agtcacggtt gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat
    19201 ggtgaaccgg atagccaaac ggcgaccaga gtttattttt cgcatagccc cagtactctt
    19261 tacccaaatt ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac
    19321 ccacacgatg cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca
    19381 gttgatccca gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt
    19441 tgctgtaaca ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt
    19501 catcgaacaa attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa
    19561 aatctttcgg cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac
    19621 cacctgtgct gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac
    19681 ggatcgggca agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta
    19741 agtcaaaaac ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt
    19801 cacccgtttc aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc
    19861 agcgtgactt gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt
    19921 tgttattcgc accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac
    19981 gatcggcaat gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac
    20041 ccattattgc gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga
    20101 gaggaataag gttttcccca gcctgaccaa tagccgccac acaggtttcc ggactggtca
    20161 atcgacaaat ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt
    20221 ccaggctaac tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga
    20281 taataatgac gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg
    20341 catcgacgac taaatttcct ttggtaaaag tagaaagtga ggtgatattt acgcgagaac
    20401 tacagggggc accagatcca gttaatgggc cggtagcaaa gactaattta ttcgcttcat
    20461 cgaaaggttt cgtgcctagc ggtacttcgt catacataat tttgtagccg aagcccatgc
    20521 caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct gtcgtgagat
    20581 tgactcttaa tatattacct gtccaaccgt tagccatgat ttttttcttt gcaagattca
    20641 cacagtaata tctttccact cgataatttt taacgcccct g
//
LOCUS       DADLSE010000065        20647 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLSE010000065 DADLSE010000000
VERSION     DADLSE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.065"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(370..1065)
                     /gene="aqpZ"
                     /locus_tag="JN692_002346"
     CDS             complement(370..1065)
                     /gene="aqpZ"
                     /locus_tag="JN692_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7264640.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLINIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1490..3148
                     /locus_tag="JN692_002347"
     CDS             1490..3148
                     /locus_tag="JN692_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7264641.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(3145..4101)
                     /locus_tag="JN692_002348"
     CDS             complement(3145..4101)
                     /locus_tag="JN692_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7264642.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4252..5367
                     /gene="macA"
                     /locus_tag="JN692_002349"
     CDS             4252..5367
                     /gene="macA"
                     /locus_tag="JN692_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7264643.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5364..7311
                     /gene="macB"
                     /locus_tag="JN692_002350"
                     /pseudo
     CDS             5364..7311
                     /gene="macB"
                     /locus_tag="JN692_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(7384..7608)
                     /gene="cspD"
                     /locus_tag="JN692_002351"
     CDS             complement(7384..7608)
                     /gene="cspD"
                     /locus_tag="JN692_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7264644.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7931..8251
                     /gene="clpS"
                     /locus_tag="JN692_002352"
     CDS             7931..8251
                     /gene="clpS"
                     /locus_tag="JN692_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7264645.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8282..10558
                     /gene="clpA"
                     /locus_tag="JN692_002353"
     CDS             8282..10558
                     /gene="clpA"
                     /locus_tag="JN692_002353"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7264646.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTAALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10961..11048)
                     /locus_tag="JN692_002354"
     tRNA            complement(10961..11048)
                     /locus_tag="JN692_002354"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11012..11014),aa:Ser,seq:gga)
     gene            complement(11302..11520)
                     /gene="infA"
                     /locus_tag="JN692_002355"
     CDS             complement(11302..11520)
                     /gene="infA"
                     /locus_tag="JN692_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7264647.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11805..12509)
                     /gene="aat"
                     /locus_tag="JN692_002356"
     CDS             complement(11805..12509)
                     /gene="aat"
                     /locus_tag="JN692_002356"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7264648.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12551..14272)
                     /gene="cydC"
                     /locus_tag="JN692_002357"
     CDS             complement(12551..14272)
                     /gene="cydC"
                     /locus_tag="JN692_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7264649.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14273..16039)
                     /gene="cydD"
                     /locus_tag="JN692_002358"
     CDS             complement(14273..16039)
                     /gene="cydD"
                     /locus_tag="JN692_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7264650.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(16162..17127)
                     /gene="trxB"
                     /locus_tag="JN692_002359"
     CDS             complement(16162..17127)
                     /gene="trxB"
                     /locus_tag="JN692_002359"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7264651.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17672..18166
                     /gene="lrp"
                     /locus_tag="JN692_002360"
     CDS             17672..18166
                     /gene="lrp"
                     /locus_tag="JN692_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7264652.1"
                     /translation="MVDSKKRSGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18301..>20647
                     /gene="ftsK"
                     /locus_tag="JN692_002361"
     CDS             18301..>20647
                     /gene="ftsK"
                     /locus_tag="JN692_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7264653.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIALFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQPQYQQPQYQQPQQPVAPQQQ"
BASE COUNT         5226 a         5145 c         5480 g         4796 t
ORIGIN      
        1 gtgctaaggc atcccggaag aaattctgac atactttatg aacaggcata gcagtgatct
       61 cattgaattg ttggcacaaa tcagtagatc acataatgct atgtctgtct cgttttctgg
      121 ggattcctca gcccctgcac ccccgggctc tggcgaacaa aatcgccgct gcgcggtacc
      181 ttcagcttat ccccttcggc aaccgggtcg ggcgcgaggt aacgtcctgt aagacgcgcc
      241 ctcagcccac atccatgtgg gctgccccgg ccttcgggga acgcttcagc gattttgacg
      301 ccaccaatac ccgtgcggtt tgtaatgtcg aagataaaac aaccagggca gataaaatgc
      361 caatgctaat taatcacgct tttccagcag agtccggtaa atcagaccac cgataatgcc
      421 gccgacaatt ggcaccaccc agaagaacca cagttgttct aatgcccagc cgccctggaa
      481 gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg ttagtcaccg gaatattgat
      541 taagtgaatc agggttaagg ccagaccaat agcgatcggc gcaaaacctg ccggagcgaa
      601 tttgtcggtt gcaccgtgga tcactaacag gaagcccgca ctcaatacca gttcaactac
      661 cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg ccataaccgt tagaagcaaa
      721 accgctggct gccgcgtcaa aacccgtttt accactggca attaaataca gcagcgccgc
      781 tgcaacaata ccgccgacaa cctgggcaat tacgtagcca acgacttctt ttgccggaaa
      841 acgtccgcca gcccataaac caatagtgac cgccgggtta aaatgaccac cagaaatatg
      901 accaacagcg aaggccatcg tcagaacggt caaaccaaat gccagcgcca cgccggcaaa
      961 acctatgcct aattccggga agcctgcggc cagtacagca ctaccacagc caccaaaaac
     1021 aagccagaaa gtaccaaaac attcagctgc taattttctg aacatatcca cctcaattaa
     1081 aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg tcgtaaaaaa tgacgacgga
     1141 aataatgcgc ggctatttta aaaacgaagg cgagtcattc accagataaa taaatccagt
     1201 aaatttgatt tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt
     1261 agtgcaagca aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta
     1321 tttgattttg ttgtttttgc gggcttatca gcaagaggga gtataacgcg attattcgct
     1381 catttttcag acaattgcca tgcttaaatg tgatgtcatc acgtattagc aaggcctttc
     1441 ccgttatact gccagcgtaa aggataagtc acatatttct ggaggggata tgattcttga
     1501 gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc tcgaacaaaa
     1561 caacgtcctg attggggaga acgcgtgggg taaatccagc ttgctggacg ccttaaccct
     1621 gctgctatcg ccagaatcag atctctacca ttttgagcgc gacgatttct ggttcccgcc
     1681 gggagatatc aacgggcgag aacatcatct gcatattatt ttgaccttcc gcgaatcgct
     1741 gccaggccga catcgggttc gccgttatcg gccgctggaa gcgtgctgga cgccatgcac
     1801 cgatggctat caccgtattt tttatcgtct ggaaggggag agtgcggaag acggcagcgt
     1861 gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgttg aggatattaa
     1921 cgatcaggca cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt
     1981 tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg
     2041 ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg caaaatctct ctgatggaca
     2101 gattcgtcag ggactttccg caatggtgca gttgcttgag cattatttct ctgagcaggg
     2161 ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg
     2221 gcgctatctg gacatcatca accggatgat tgaccgacct ggagggcgct cgtatcgggt
     2281 tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattgc gactggataa
     2341 agacgcgcgt ccgttattgc taattgaaga tccagaaacc cgcctgcatc cgattatgct
     2401 ttccgttgcc tggcatctgt tgaatctttt gcctttacag cgtattgcta ccaccaactc
     2461 tggcgagctg ctttcgttaa ctccagtgga acatgtttgc cgcctggtgc gtgaatcttc
     2521 tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc accgaagaca gccgccgcat
     2581 ctcttttcac attcgtttta atcgtccgtc atcgctgttt gctcgctgct ggttgctggt
     2641 ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgtcagtgcg gacatcattt
     2701 tgatgccgaa gggatcaagg tcattgagtt tgcccagtcc gggctaaagc cactggttaa
     2761 atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg aagcagggaa
     2821 gaaatatgcc gctacggtac gtagcctgtt gaataacgat cgggaagccg aacgagaaca
     2881 tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg gattttccga
     2941 tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctac gcaaaattat
     3001 ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg caatggaggc
     3061 aggacgtcgt ggtgtggact ccgtaccaac gctgctgaaa aaaatgttct cacgcgtgct
     3121 gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt gtggctgaat
     3181 agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg cgatttcggc
     3241 aatctctttt cgtgctatct gtgctggaag gcggtaatgg cgttcagcat cacatacgcc
     3301 gccaaccgac tcccagaacg cgttgtaatc agcatggatc ttgccttctt tatcgcgata
     3361 acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct ctacctgcaa
     3421 acgttgggca aacagacagg ccgcttccat cacgaggcgt ttgggaaata gcccgtggca
     3481 ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc cttgcagtcc
     3541 gccaataaac atcgttcttt tcccctgata ttcacacagg gtaaacgtga tctctgccag
     3601 aggaatacct tcgctgttgc ggaacaggat tgtgctgtca ccttctttat ccattgagat
     3661 catcatggtc agctcaagcg tgaactgctc accgtttttg ccttccagtt tagccagttg
     3721 cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacacc cgcggagtaa
     3781 cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta
     3841 agggcgatgc agacgcacag gcagtcgcgg ctggcgcgtt aacaacacat tgagattagg
     3901 ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggcataatca acgagcgcag
     3961 caaaaatttc tggcgaaaac tacagcgatg ccagaattta cccggccgac actgtccacg
     4021 tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa aggtccgttc
     4081 agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt tcaccattgc
     4141 gtatcttaac caaacatcaa tagtgtgatt actaacgtaa attttagggt tttgttgata
     4201 tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt tatgaaaaag
     4261 cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc cggactgatt
     4321 acgttatgga gaattcttaa cgcacccgtg ccgacttatc agacactgat tgtgcgcccc
     4381 ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg taaggttgac
     4441 gtgggcgcgc aggtcagcgg tcagttgaaa acgctgtcgg tggcgattgg cgataaagta
     4501 aaaaaagacc agcttttagg ggtcattgat cctgaacagg ctgaaaacca gatcaaggag
     4561 gtcgaagcaa cgctgatgga gctacgcgcg cagcggcagc aggcggaagc ggagctgaaa
     4621 ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac aaacgcaagc ggtttcactg
     4681 caggatctcg ataccgccgc gacggagatg gctgtgaaac aggcgcaaat tggcaccatt
     4741 gacgcgcaaa tcaagcgcaa tcaggcctct ctcgatacgg ctaaaaccaa tctcgattac
     4801 acccgtatcg ttgccccgat ggccggggaa gtgacgcaaa tcaccactct gcaaggccag
     4861 acggtgattg ccgcacagca agcaccgaac attctgacgc tggcagatat gagcactatg
     4921 ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc
     4981 tggtttacgg tgcttggcga tccactgacg cgctacgagg ggcaaatcaa ggatgtgcta
     5041 ccgacgccgg aaaaggtaaa cgacgctatt ttctattacg cccgttttga agtacccaac
     5101 cccaatggtt tgctgcggct ggatatgacc gcgcaagtgc atattcagct caccgatgtg
     5161 aaaaatgtgt tgacgatccc tctgtcggcg ttaggcgatc cggttggcga taatcgttat
     5221 aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt
     5281 aacgataccg atgttgagat tgtcaaaggg ctggaagcgg gcgatgaagt ggtgattggt
     5341 gaggccaaac caggagctgc acaatgacgc ctttgctcga attaaaggat attcgtcgca
     5401 gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcagcctc aatatttatg
     5461 cgggtgagat ggtcgcgatt gttggcgctt cgggttccgg taaatcgacc ctgatgaata
     5521 ttctcggctg cctggataag gccaccagcg gcacctatcg cgtagccggt caggatattg
     5581 ccacgctgga cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc tttattttcc
     5641 agcgttacca tttgctttcg catttaaccg ccgagcagaa cgttgaagta cccgccgtct
     5701 atgccggtct tgagcggaaa cagcgactgc ttcgtgccca ggagttgctg caacggctgg
     5761 ggctggaaga ccgtacagag tattatccgg cacagctttc gggtggtcag caacagcgcg
     5821 tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat tcttgccgat gaaccgaccg
     5881 gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc
     5941 ccgggcatac ggtgattatc gtcacccacg atccgcaggt cgctgctcag gccgagcggg
     6001 tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg
     6061 ttgctggcgg gacggagcct gttgtcaata cagtgtctgg ctggcggcag tttgtcagcg
     6121 gttttaacga ggcgctgacg atggcatggc ggacgctggc agcgaataaa atgcgtactt
     6181 tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg
     6241 gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tattggtacg aatactattg
     6301 atgtctatcc cgggaaagat tttggcgatg acgatccgca atatcagcag gcgctgaagt
     6361 acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct
     6421 cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc aatggcgtga
     6481 gcggcgatta ttttaatgtc tatggcatga cacttcagtg aaggaaacac ctttaatcag
     6541 gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca gaaatactcg ccgccagctt
     6601 ttcccccata aagcagatgt ggttggcgag gtgattctgg tcggcaatat gcccgccaga
     6661 gtcattggtg tggcggaaga aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc
     6721 tggctacctt acagcacgat gtccgggcga gttatgggcc agtcgtggct taactccatt
     6781 actgtcaggg tgaaagaagg atttgacagc gccgaggcgg aacagcaact cacgcgttta
     6841 ctttcactgc gccacggtaa gaaggatttc tttacctgga acatggacgg cgtcttgaaa
     6901 actgttgaaa agaccacacg tactttacaa ctgtttctga cgctggtggc ggtgatttcg
     6961 ctggtggtgg gcggtattgg tgtaatgaat attatgctgg tgtcagtgac cgagcggacg
     7021 cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttc
     7081 ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt tgggaataac actgtcactg
     7141 ttaattgctt tcaccttgca gcttttctta cccggctggg agattggttt ttcaccgttg
     7201 gcgctgctgc tggcgtttct ctgctcgacg gccaccggga ttttatttgg ctggttaccc
     7261 gcacgaaatg cggcacgact ggatccagta gatgctctgg cacgagagta atttttgaga
     7321 taaaaatgcc agccgatcgg gctggcattt tgcctttagg atgtacacaa tgagacagaa
     7381 gagctatgcg actgccgctt ctacttcgac gggcacaata acactggcgt gattgccttt
     7441 tggcccctgg tggacatcaa actgaacgga ttgtccagct tttagcgttc tgtaaccatc
     7501 catctgaatg gtggaataat gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa
     7561 accaaaccct ttggcattgt tgaaccactt aacagtaccc ttttccatgc ttcgacatcc
     7621 ttcgcaaatc ttatacaagt aagatggaat aaaccggggt cagagagggg gctgttcaaa
     7681 acctcgccaa ctctagaaat acaatttaga gaattagggc gagccgtcaa gcatttgaca
     7741 ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt gacaggaatg
     7801 tgacagatgt cgctgatgcc aacgatagat gatagttatc tatcatgtgg agtagattgg
     7861 tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc gataaccgta accgaagatg
     7921 ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg cggaagaaaa
     7981 agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca atgatgatta
     8041 cactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg
     8101 tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgcg gagtctttac
     8161 cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg agaatgagca
     8221 tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc
     8281 tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag cgcgcgagca
     8341 ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta acccatctgc
     8401 ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg ctccgtcagg aactggaagc
     8461 ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc
     8521 gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc catgtccagt cctccggtcg
     8581 caatgaggtt accggtgcaa acgttctggt cgctatcttc agcgaacagg agtcgcaggc
     8641 ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat gtggtgaact ttatctctca
     8701 tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc caaacagcga
     8761 agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga accagcttgc
     8821 gcgtgtgggc ggaatcgacc cactaattgg tcgtgagaaa gagctggagc gtgctattca
     8881 ggttctctgc cgtcgccgta aaaacaaccc gctgctggtg ggggaatctg gtgtcggtaa
     8941 aaccgcgatt gcagaaggtc ttgcctggcg aattgttcag ggtgatgtgc cggaagtgat
     9001 ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca caaaatatcg
     9061 cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg acactaacag
     9121 catcctgttt attgatgaga tccacaccat tatcggtgcg ggtgcagcgt ctggtggtca
     9181 ggtcgatgcg gctaacctaa tcaaaccgtt gctctccagc ggtaaaattc gcgtaattgg
     9241 ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg
     9301 cttccagaaa attgatatta ctgaaccgtc gatcgaagaa actgttcaaa tcatcaatgg
     9361 cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc
     9421 ggcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata aagccattga
     9481 tgttatcgac gaagcgggcg ctcgcgcacg tctgatgccg gtaagcaaac gcaagaaaac
     9541 cgttaatgtg gcggatattg agtccgtggt ggcccgtatt gcacgcattc cagagaagag
     9601 tgtttctcag agtgaccgcg ataccctgaa aaacctcggc gatcgcctga aaatgctggt
     9661 cttcggccag gataaagcca ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg
     9721 tttaggtcac gaacataaac cggtcggttc gttcctgttt gccggtccta ccggggtcgg
     9781 gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagcttc tgcgctttga
     9841 tatgtccgag tatatggaac gccataccgt cagccgtctg attggtgcgc ctccgggata
     9901 cgttggtttt gatcagggcg gtttgctgac cgatgcggta atcaagcatc cacatgcggt
     9961 gctgctgctg gacgaaatcg agaaagcgca tccggacgtg ttcaatattc tgttgcaggt
    10021 gatggacaac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt
    10081 gctggtgatg accactaacg ccggggtacg ggaaactgag cgcaaatcca ttggtcttat
    10141 ccaccaggat aacagcaccg atgcgatgga agagatcaag aagatcttta caccggaatt
    10201 ccgtaaccgt ctcgacaaca ttatctggtt cgatcatctg tcaactgacg tgatccatca
    10261 ggtggtggat aaattcatcg tcgagttgca ggttcagctg gatcagaaag gtgtttctct
    10321 ggaagtgagc caggaagcgc gtaactggct ggccgagaaa ggttacgacc gggcaatggg
    10381 cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg ccaacgaact
    10441 gctgtttggt tcgctggtgg acggcggtca ggtgacggct gcgctggata aagagaaaaa
    10501 tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag cgcattaatc
    10561 tgattgtcag gtaggttggt gaagtccgta atctcgtcag gggttacgga ctttttattt
    10621 atggggtgga acggtgaaaa ccctattttt ggaggtgaag gtaagttgtt gataattagt
    10681 gctgctggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt tttgctttat
    10741 taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaatcg
    10801 acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacaggcagc ttagaaattc
    10861 acaggtaaca tactccaccc gcccaccatg ttcactgccg tacagacaga taaaatgcga
    10921 aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcggtgag ggggggattc
    10981 gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac
    11041 acctcaccaa attgttttgc taccaaacct catgggtggc aacggggcgt tactataggg
    11101 agttggagta aaacggtcaa gaagaatttt aatgataatt attgtttgct catactgtaa
    11161 acaacttgtg cagtatatct acatcgagac aagttatgga cttatacttc caaagtactt
    11221 catacatatc acaaaataaa aaggccggtt aaaccgacct tttactcgtt ctttctcttc
    11281 gcccatcagg cggtaaaaca atcagcgact acggaagaca atgcggcctt tgctcaggtc
    11341 gtacggggtc agttcaacag tcactttgtc gcccgtcagg atgcggatgt agtttttgcg
    11401 cattttaccg gagatgtgtg cagtaaccac gtgaccgttt tctaactcta cgcggaacat
    11461 ggtattaggc aacgtttcaa gaacggtacc ttgcatttca atattgtctt ctttggccat
    11521 ctaatcctct ggggtatcac taccgtaatt tgaaccggca agataatgcc gaagttctgt
    11581 aaataagtaa agatttgcgc gctaaatcgc aacaaacagg ttcggcacat tactccgaaa
    11641 acacacggct aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg
    11701 gcgttacctg acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta accaactctg
    11761 cgaccgcaat tataacactc tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa
    11821 aacaagcacc gtggtaccca gaaattattc ggcaatcgtc cgaggcgcat ttgattgaga
    11881 taattaaggt aatcccggcg gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta
    11941 aggacctggc agtcgataag cttaccgcca tgaccgataa attcctcaca gaataccaga
    12001 agcgccgttt tagacgcatt ttccatccgg ctgaacatgg actcgccaca aaatagcgtt
    12061 ccctgggcca cgccgtacat accgccgaca agctcatctt cacgccagac ttcaatggag
    12121 tgggcatgac cgagttcgtg aaggcgatgg taggcttcga ccacgccacg cgtgatccag
    12181 gttccttctt cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc gtaattcatc
    12241 gtgacacgat agggcgagcg tttatgaaat cgcttcatac tacggctgat atgcagtgat
    12301 tctggccata gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc gcctggagaa
    12361 aaccacggaa aaataccacg ctggtaagcc attaacaggc gcgcagggct aagatcgccc
    12421 ccaagtgcca gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg gaaggctatt
    12481 gaatggcgag aaagctgaac caggcgcatg accgcaaaac tccacgcaag tcggatcgtt
    12541 caataatagc ttacaaaccc tgcttgaact ggtaataacg cccctgtctg gcaagcagtt
    12601 ctgcgtgagt accttgctca ataatttgcc cgttgtccat cactattatt tgttggaaac
    12661 gagagagtcc gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc atcatttctg
    12721 caagcaattc aaggatctgg ctttcggttg tggcatctaa gccttcggta ggttcatcca
    12781 gcaacaccag tggcgcatca tgtaacagcg cacgggcgat agccagacgg cgcagttcac
    12841 caccggagag ctggcgtccg ccttcaccta accaactgtt gagacctgca tcctcgagca
    12901 gcttctccag accaacgcga cgcaagatct ccgacagagc ctcatcacta ctgccaggcg
    12961 aggcgagtaa cagattatca cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa
    13021 cgctgatggt ctgtcgtaga gccgcttcat tcaggctggc tatggggcta tcgttaagca
    13081 aaatctcgcc ctgttgcggg tcccatgcgc gggtcagcag ttgtaacagt gttgatttgc
    13141 cgcatccggt tcgcccgaga atcgctatat gttccccggc gtttacctga agagaaatcc
    13201 ctttaagtgc ctgttgggat tgctccggat aagtgaactg aacatcccgt aatgtcagcg
    13261 aaacgcgatc ggcaacacga gtttgggtat cagggaaggt gacctccggt ttttgatccg
    13321 ttaagtcaga gatacgtacg gcagaggcaa tgacttgccc cagatgctga aatgcacccg
    13381 ttactggtgc cagtgcttca aacgcggcta acgcacaaaa gacaaacagg gcaattaatg
    13441 cgccgggttg agcattgccg ccaacgccgc cagacgccat ccacagcatc aggatcaccg
    13501 ctaacgcgcc aatgagcagc attatcgctt gcgacaatgc ggtcagttca gattgacggc
    13561 gttgcgcttc cagccattga atttctgtat tctctagttg cgtgcgataa cgatcgctgg
    13621 caccaaaaat ggtcagctca gcttgccctt gcagccaggc cgtcagttgt tggcgatact
    13681 gtccgcgaag atgagtcaga ttttgcccgg tgctttttcc cgcacgataa aacagcggtg
    13741 gcatcaggaa aagcgtcagt aacataacgc tgcccagcgt aaaggcgagg gtgaaatcaa
    13801 ggaaacttaa cccgattgtc accaccataa tcaccacaaa agcgcccacc agcggcgaga
    13861 taacgcgcag gtaaagatga tcgagcgtat caacatccgc caccacgcgg ttgagcaatt
    13921 cgccctgacg atagcgcgcc agtccggcag gggagagggg caacaatttg ctgaaggtgt
    13981 aaatgcgcag atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat
    14041 aacgcccggc agtacgggtg attgcggcac cacgcacgcc cgcagcgggt agcatatagt
    14101 tgaagctgta cagtccggca acccccgcaa ccgctgaggc cgagaggaac cagccggaaa
    14161 gtgtcaacag accgatactg gcgagcagcg tcacaattgc cagcacaata ccaagactta
    14221 acatccattt atgacgttta tacagtgcca gatagggtag caaagcgcgc atttaaatct
    14281 cctcctgacg atgggccagt aatgtggcga atgggccacc agccacactt aattccgcgt
    14341 aacgtccttg ctcaataatc cggccatcct gcataaccca aatgacatcc cagtcagcaa
    14401 gatcttctaa ctggtgggtg accattaacg ttgtctggcg cagagaggcg gcattcagcg
    14461 cctccattac gcgctgttca ctgtgagcat caaggctggc agcgggttca tccaacagta
    14521 atagcgaaca gggatttagt aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg
    14581 aaaggcgggc agcctggtcg ccaacaggtg tatcaacgcc ttgtggcagg agcggtagaa
    14641 actcgctgac ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg gcatcaggtc
    14701 gcgccagtag tacgttatcc cgcaattttg ctgccggtaa ttgtgggttt tgcccaaccc
    14761 aggagagatg tttacgccat gattctggtg ataaatcacg taattctatc ccgttgattc
    14821 gtaatgatcc ctgatatgag agaaaaccag aaagcgcgtt cagcagtgag cttttacctg
    14881 aaccgctgcg gccaaccaac actgcacgtt ggcctgctgg caaagtaaag ttcagcggtc
    14941 cggccagcgt tttaccttcc ggcgacgtga taaacagatc ctcggcctca atggtcagcg
    15001 gatcggtcaa tgctaattcc gcctcaccgc gctgcggatg ggcgagcggg gtttccataa
    15061 acgttttcag actgtcagct gcaccaacag cctgggcttt agcatgataa aacgtaccga
    15121 gatcgcgtaa tggctggaaa aactctggcg caaggatcag ggccagaaaa cctgcagcca
    15181 gcgtcacccc agtatcgtag tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt
    15241 agaccgccac cagagcaatt gacagcgagg taaaaaattc gagaatgccg gaggataaaa
    15301 acgccagtcg tagcacttcc attgtccgtt ggcggaaatc ttccgaagca gaacgaatac
    15361 tttcaatttc agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat
    15421 cgaggaaatg cccacttaag cgagcaagag cgagaaagtt acgccggtta gcatcggcag
    15481 cccccattcc aaccagcgcc ataaacaacg gaattagcgg tgctgtgccc agcagaatga
    15541 gcgccgcagc ccagttagag gggaagatcg ccaccacaat cagcaacggc accgacactg
    15601 ccagcgccat ttgcggcaga tagcgtgcat agtaatcatg catatcgtca atttgctcga
    15661 gtaccagcgt cgcccagctc cccgcaggtt taccctgaat ccacgctggc cctgcttgtt
    15721 gcagacggtc gagaacctga cggcggatgg caaagcggat atgctgcccg gcgtgataac
    15781 ccacccgttc gcgtaaccag accacccatg cgcgcaatac aaaggtcaga accagtaacg
    15841 taaagggaag cagcagggct tcacggggaa tattctccat aatcatatgt tgcagaattc
    15901 gcgccatgaa ccaggcctgg gcaatgatca atatgccgct tacaaagccc agcagacgag
    15961 aaatattcag ccaacgttgg gagatgacgc tttgctgttt taaccagcgg gttaactctt
    16021 tttgacgaga tttattcatt gcacgcttag caggtgagtt atcagaatta tttgcagagc
    16081 aatgttacaa cggggaaaaa ataaaggcga cccatagtcg caaggtgtcg ccttctttac
    16141 ttttgttact gatttgtaaa attattttgc gtcagctaaa ccatcgaggt agcgttccgc
    16201 atcaagtgct gccatgcagc cagtaccggc cgaagtaatg gcctggcgat aaatgtgatc
    16261 catcacgtcg cctgcggcaa agacgccagg aatgctggtc tgggtggcat taccatgaat
    16321 acccgactgt actttgatgt agccgttttc cagttccagc tgcccttcga aaatcgcagt
    16381 attcgggctg tgaccgatag caacaaacag accggcaacg tcgagtgact cgatgttatc
    16441 gctgttttgc gtatcgcgca gacgaacacc agtgacaccc atttgatcgc cggtcacttc
    16501 ttccagcgta cggttggtgt gcagaatgat gttgccgttc tccactttat ccatcagacg
    16561 cttaataagg attttttccg cgcggaaacc gtcacggcgg tgaatcagat gcacttccga
    16621 agcgatgtta gacagataca gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc
    16681 aactttctgg ttgcgataga agaaaccgtc gcaggttgca caagcagaaa ccccacggcc
    16741 tttaaaggct tcttcagagg gcaggccgag atagcgtgca gaagctccgg tggcaataat
    16801 cagcgcgtcg caagtgtatt cgccgttatc gccattcaga cggaacggac ggttttgcag
    16861 atccaccttg ttgatatgat caaaaatgat ctcagtttca aacttggtgg catgttcgtg
    16921 catgcgctcc attaataacg gaccggtcag atcgtttgga tcaccaggcc agttttccac
    16981 ttccgtggtg gtggtcagtt ggccgccttt ttccatgccg gtaatcagca caggttgcag
    17041 gttggcgcgc gccgcgtaga cagcagcggt gtatcccgcc gggcctgaac ccaggataag
    17101 cagtttactg tgtttggtcg tgcccatgag atccccatag ttgttggcag acaatgggca
    17161 ggattgtagg gaatttacag acgtaaaaaa agagtatgac gattttgtta acaatttgtg
    17221 caatcggcag catcgataag caggtcaaat tctcccgtca ttatcacctc tgctacttaa
    17281 atttcccgct ttataagccg attaaatgat gaataaacgc ccctgttaat gaatatctgg
    17341 catgttgtac taaaaatcga tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat
    17401 tcgaggaaga aaaaaaacag tattcttata tgcgcataac catgtatgta aataccatgt
    17461 ttaccgtgct agtgaaatct acgtatggcg tggacagacg ccattcgtga tgtcgatagc
    17521 tgccacaagg caacggtctt ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat
    17581 gatttcggtc tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg gtcagacagg
    17641 agtagggaag gaatacagag agacaataat aatggtagat agcaagaagc gctctggcaa
    17701 agatctcgac cgtatcgatc gtaacattct taatgagttg caaaaggatg ggcgtatttc
    17761 taacgtcgag ctttctaaac gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg
    17821 tcggctggaa agacaagggt ttattcaggg ctatacggcg ctgcttaacc cccattatct
    17881 ggatgcatca cttctggtat tcgttgagat tactctgaat cgtggcgcac cggatgtgtt
    17941 tgaacaattc aataccgctg tacaaaaact tgaagaaatt caggagtgtc atttagtatc
    18001 tggtgatttc gactacctgt tgaaaacacg cgtgccggat atgtcagctt accgtaagtt
    18061 gctgggggaa accctgctgc gtctgcctgg cgtcaatgac acacggacat acgttgttat
    18121 ggaagaagtc aagcagagta atcgtctggt tattaagacg cgctaacacg gaacaggtgc
    18181 aaaatcggcg tattttgatt acactcctgt taatccatac agcaacagta ctggggtaac
    18241 ctggtactgt tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg agagcctttc
    18301 ttgagccagg aatacactga agacaaagaa gtcacattga caaagttaag tagcggccgc
    18361 cgccttctgg aggcgttgct gatccttatt gccctgtttg ccgtctggtt gatggctgcc
    18421 ttactaagct ttaacccttc ggaccccagc tggtcgcaaa cggcctggca tgaacctatc
    18481 cataatttag gtgggatgcc cggtgcgtgg ttggcagata cgctgttctt tatttttggc
    18541 gtgatggctt acaccattcc cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat
    18601 cagtccagcg acgaatacat tgattatttt gccgtttcgc tacgcatcat tggcgttttg
    18661 gcgctcatcc ttacctcctg tggtctggcg gcaatcaacg ctgacgatat ctggtatttt
    18721 gcctccggtg gcgtcattgg cagcttacta agcactacgc tacaaccact gctacacagt
    18781 agcgggggaa ctattgcgct gctctgcgtt tgggcagcgg gcctgacgtt gttcaccggt
    18841 tggtcatggg tgactattgc tgaaaaactc ggcggctgga ttttaaacat tctcaccttc
    18901 gccagtaatc gtacccgtcg cgatgatacc tgggtcgatg aagatgagta tgaagacgac
    18961 gaagagtatg aagatgaaaa tcacggcaaa cagcatgaat cacgccgtgc ccgtattctt
    19021 cgtggcgcgc tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc gatggggcgg
    19081 caaacagacg ctgcgttgtt ctccggtaag cggatggatg atgacgaaga gattacctac
    19141 actgcacgcg gtgtggctgc cgacccggac gacgtcctat tttcgggcaa tcgtgcaacg
    19201 cagccagaat atgacgaata cgatccatta ttaaacggtg cgccaattac cgaacctgtc
    19261 gctgtagcag ctgctgctac cacggcgaca caaagctggg ctgcgccggt tgaacctgtg
    19321 actcagacgc cgcctgttgc ctctgttgat gttccacctt cgcaacctac agtagcctgg
    19381 cagcctgtac cgggtccaca aacgggagag ccggttattg ctcctgcacc ggaaggttac
    19441 ccacagcagt cacaatatgc gcagcctgca gtgcaatata atgagccgct gcaacaacca
    19501 gtacagccgc agcagccgta ttatgcacct gcagctgaac agcctgcgca acagccgtat
    19561 tatgcccctg cgccagaaca accggtggca ggtaacgcct ggcaagccga agagcagcaa
    19621 tccacttttg ctccacagtc tacataccag actgagcaaa cttatcagca gccagccgct
    19681 caggagccgt tgtaccaaca gccgcaaccc gttgaacagc agcctgttgt ggagcctgaa
    19741 cccgttgtag aagagacaaa acccgcgcgt ccgccgcttt actattttga agaagtggaa
    19801 gagaagcgag cccgtgaacg tgaacaactt gcggcctggt atcaaccgat tccagaaccg
    19861 gttaaagaac cagaaccgat caaatcttcg ctgaaagcac cttctgttgc agcagtacct
    19921 ccagtagaag ccgctgccgc tgtttctccg ctggcatctg gcgtgaaaaa agcgacactg
    19981 gcgacggggg ctgccgcaac cgttgccgcg ccagtcttca gtctggcaaa tagcggtgga
    20041 ccgcgtccgc aggtcaaaga ggggattggc ccgcagttgc cacgaccgaa acgtatccgc
    20101 gtgccaactc gtcgtgaatt ggcgtcttac ggtattaagc tgccctcaca gcgtgcggcg
    20161 gaagaaaaag cccgtgaagc ccagcgcaat cagtatgatt ctggcgatca gtataacgat
    20221 gatgaaatcg atgcgatgca gcaggatgaa ctggcacgtc agttcgccca gacacagcag
    20281 caacgctatg gcgaacagta tcaacatgat gtgcccgtaa acgcagaaga tgcagatgct
    20341 gcggcagagg ctgaactggc tcgtcagttt gcgcaaactc aacaacaacg ttattccggc
    20401 gaacaaccgg ctggggcgaa tccgttctcg ctggatgatt ttgaattttc gccaatgaaa
    20461 gcgttgctgg atgatggtcc acacgaaccg ctgtttacgc caattgttga acctgtacag
    20521 cagccgcaac aaccggttgc accgcagcag cagtatcagc agccacaaca gccggttgcg
    20581 ccacagccgc agtatcagca gccacaatat cagcagccgc aacaaccggt tgcgccacag
    20641 cagcaat
//
LOCUS       DADLSE010000066        20528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLSE010000066 DADLSE010000000
VERSION     DADLSE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.066"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..686)
                     /locus_tag="JN692_002362"
     CDS             complement(<1..686)
                     /locus_tag="JN692_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7264654.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARG"
     gene            860..1573
                     /locus_tag="JN692_002363"
     CDS             860..1573
                     /locus_tag="JN692_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7264655.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            1605..2363
                     /locus_tag="JN692_002364"
     CDS             1605..2363
                     /locus_tag="JN692_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264656.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            2409..3758
                     /locus_tag="JN692_002365"
     CDS             2409..3758
                     /locus_tag="JN692_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7264657.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3758..4582
                     /locus_tag="JN692_002366"
     CDS             3758..4582
                     /locus_tag="JN692_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264658.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            4594..5153
                     /locus_tag="JN692_002367"
                     /pseudo
     CDS             4594..5153
                     /locus_tag="JN692_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            5184..6254
                     /gene="lptF"
                     /locus_tag="JN692_002368"
     CDS             5184..6254
                     /gene="lptF"
                     /locus_tag="JN692_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7264659.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYNVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            6251..7242
                     /locus_tag="JN692_002369"
                     /pseudo
     CDS             6251..7242
                     /locus_tag="JN692_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            complement(7278..8450)
                     /locus_tag="JN692_002370"
     CDS             complement(7278..8450)
                     /locus_tag="JN692_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7264660.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDVIIQAFATLKQ"
     gene            complement(8450..8698)
                     /locus_tag="JN692_002371"
     CDS             complement(8450..8698)
                     /locus_tag="JN692_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7264661.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8730..9626)
                     /locus_tag="JN692_002372"
     CDS             complement(8730..9626)
                     /locus_tag="JN692_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7264662.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            complement(9641..11328)
                     /locus_tag="JN692_002373"
                     /pseudo
     CDS             complement(9641..11328)
                     /locus_tag="JN692_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            11732..12874
                     /locus_tag="JN692_002374"
     CDS             11732..12874
                     /locus_tag="JN692_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7264663.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN692_002375"
     CDS             complement(12998..13768)
                     /gene="glcC"
                     /locus_tag="JN692_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7264664.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            14019..15518
                     /gene="glcD"
                     /locus_tag="JN692_002376"
     CDS             14019..15518
                     /gene="glcD"
                     /locus_tag="JN692_002376"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7264665.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVDVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            15518..16570
                     /gene="glcE"
                     /locus_tag="JN692_002377"
     CDS             15518..16570
                     /gene="glcE"
                     /locus_tag="JN692_002377"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7264666.1"
                     /translation="MLRECDYSQALLEQVNQAISDKMPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLLGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            16581..17804
                     /gene="glcF"
                     /locus_tag="JN692_002378"
     CDS             16581..17804
                     /gene="glcF"
                     /locus_tag="JN692_002378"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7264667.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTFKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            17809..18213
                     /locus_tag="JN692_002379"
     CDS             17809..18213
                     /locus_tag="JN692_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7264668.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            18235..20406
                     /gene="glcB"
                     /locus_tag="JN692_002380"
     CDS             18235..20406
                     /gene="glcB"
                     /locus_tag="JN692_002380"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7264669.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         4967 a         5109 c         5522 g         4930 t
ORIGIN      
        1 taaccacgcg cggtcataaa atccgtaatc gctttttctg catcaaccaa cacctgttcc
       61 agtggctgat tggcatcgat atcaaccagt tgtgcaccac caaaggttaa ctgtggcgtt
      121 atggcaatct tcctcgccag cgattcccgt ttatggtcgg gtttacgtgc acaggcaact
      181 tcaaggtcaa cattgagttt gatgaccaga tcaggcttat ggctcgccat ccagtgaaac
      241 gctttacgtt cctggcttgc cagccatgag acaaaacgac caccttcaac gttaggtggg
      301 aacaccgtac catcgtaagc gccaggaatt tggtcctgag gataacggtc ggttagaaca
      361 attaacccgc gacgacgaca ggcaagcata tgacgaaagc gcagtaagcg acgggcgaca
      421 aacgctgtaa ttaccagcgc cggaactggt ccaggcaatt tttttgctgt tttcacctga
      481 tttcgttcaa ttgttttatg taaggatttt cccatcaaag gtaattttgt cactgcacga
      541 ccgacatttc cagcctgttt tcctaaatga actctttcgg cagcaccata tttttcgaca
      601 acggtaataa gatgttcaca caccgttgac ttgcctgaac catcgctacc aataacggca
      661 attaatggag gtgttattga ctgcatgaat atatccttta atttaaatat ccattaaaaa
      721 tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc acaattaacc
      781 attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat tcaatacatt
      841 atttactgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt ccacgccagt
      901 gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca cggcaagcct
      961 ggtaaatgaa ctggcagcaa gaatgccaac agaacacatt tatctcgggc aatcgtccgg
     1021 gcgaattggc gaatggattt cacagctccc tgttattggc gcaccttgtg ggtgttatct
     1081 gcgaagtaaa gcggcacatg tacacgaaaa gccctcaaca ccgcctggca atattactgc
     1141 gctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa tgttgtgtaa
     1201 aagccagcaa ggctatctgc tcatcaccga ccgctacccg caggttgaag tgccgggttt
     1261 tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga taaaaatgtt
     1321 aaggcagcgc gagttgaagc tgtaccaatg gatggcgtct tatttacccg tattgttgat
     1381 tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc aactggcagc
     1441 gttacaggaa aaaatcgccg ttacgccgca actgacattc aatggcgcca agatccttga
     1501 actggatggt cggcaaccag ccgatgaaat tatgcaagcg tcactacgcg caattcacgc
     1561 cgcgctttcc tgatcaccaa aatggatagg ggatgattta acgaatggat gcactacaaa
     1621 cacaaactgt taatagtaca accgcgccgc agcccaacta cattccgggg ctgattgcgg
     1681 tggtcgggtg tgatggcacc ggtaaatcca cgctgaccac cgacctggtg aaatcgctgc
     1741 aacaacactg gcaaaccgag cgccgctatc tggggctgct ctccggcgaa gacggcgaca
     1801 aaatcaaacg attgccgttg gttggcgtct ggctggaacg gcgactggcg gccaaatcct
     1861 cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg attatgtact
     1921 gcttctcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg caaagtggcg
     1981 ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat tatgacggac
     2041 cggggatcgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg cagcgcgaac
     2101 ggcgtttata ccaaaaaatg gcgcaatacc gtcctgaatt aatcattcgt ttggggattg
     2161 atattgatac tgccatctcc cgcaagcctg accatgagta tgccgagctg caggacaaaa
     2221 tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt gattccagag
     2281 cgccctacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt gccatcgttt
     2341 ctgaccgccg aagtttaact taaggctggc gggatatttt ttactatcaa cgataaggtc
     2401 aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg tttcgcacca
     2461 ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcga gagcgccttg ctggggctgt
     2521 tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta ctggtgattg
     2581 tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg cagctggtgg
     2641 ttcagtacgg aacgccagcc ttgaccaaca ataatccgca gcaatttcgc aatgtcgtct
     2701 cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc ggcattgcct
     2761 tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg ctggcggcgc
     2821 tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt ctgcgtgcgg
     2881 tagatcgctt tgatttgatt gctgtacagc aggcgacgaa accttttctg cgcgcagcgg
     2941 ggagcgtcgc agcctggtat tttgactttg gttttgcggg ttttgttatt gcctggtacg
     3001 tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcgcgaa ttacgccgta
     3061 gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac attaaaggcg
     3121 cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg cgtaactcgt
     3181 gcagcacagt gttggtgggg atcgtgttag gacccgctgc cgccgggtta tttaaaatcg
     3241 ccatgacatt cttcgacgcc gccggaacgc cagcgggttt gctgggtaaa agtttttacc
     3301 cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggt gtgaagtccg
     3361 gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc gtcggcaagc
     3421 cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg attcaggtga
     3481 tgctgggcgc aattgtgatt tcgatgctgg gcttcccgca ggagtcattg ctattgatgg
     3541 cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc tacatcgtat
     3601 tgctgttcat gttctgtcat ctgtttggtg tgctgggagc agcgtttgct tacttcggtg
     3661 ggcaatgtct ggacgtggcg ctctcgttaa ttccaacctt aaaggcattt tttcagcgcc
     3721 attccttgct ttataacgca gccggagaga aatcctgatg atgagaaaat atttcccgct
     3781 tgaggcgagc gagcgtctat ttgttgcgat tgaagaagat gatgtcgttg atgcacaggt
     3841 tagtttgccg ccgactattg cactaagttg taccaccgaa atcattcatg ataattacgc
     3901 gttatgcctg caattctggc tcaatggcgt tgatcgtcag gaattactgc gtttagttcg
     3961 caaacaagcc aaaggcgatg agctgactgc tgatgagcgc aaacaattta aatacatgcg
     4021 agcgcgctac aagcacctgc gttttgcgca acggctgtat ttaaagaagc atcaggccgg
     4081 atttttattc ggcaaaacga ccgtttttct ggggcgtttt caggacggct ttcgtaacgg
     4141 taagaaaaat attgtgtcgt attacggcaa cctgttacgg atttatttaa gctctccggt
     4201 ctggtcgttg gtgaactatt cattacgcca tagccagtta gaaagcgtaa gcagttttat
     4261 tgcctatcgg caaaagcaaa tgcataccct gaaagagatt attgccaaac cgcgactaac
     4321 cggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt attacgacac
     4381 cttaagatcg cttgatcctg aaaataaaga ggcgttacag atttcccgtt tcctcgccgc
     4441 cattaatggc ctgatgggag ataaacacga cgacatggtg gcggatgata tggaaaaccg
     4501 ccagtcgtat gatgcaccgt tggcgttgga tagcgatatt cgtcagcgct tagagctgct
     4561 gatttcgcgt tttccgctat agccggagca atgatgtcat ctcgtttgat tattgcgttg
     4621 attatcatgc tcttagcccc gagtgtgcag gcacacaatt tcgtcactgg caaaacggtt
     4681 acccctgttt atatccagga aggcggcgag ctgcttctga atagcgacga cgaaattcac
     4741 taccaaaagt ggaacagcac acaattagcg ggaaaagtcc gcattattca gtatatcgcc
     4801 gggcgtaagt cggcgaagaa aaagaactca ctgttaatta aagcggttga agcggcaaac
     4861 tttccgcagg atcgttttca gcccaccacc attgtgaaca ccgatgatgc catctttggt
     4921 actggctatt ttgttgttgg caaaatcgaa aaaaataaac gcgctatccg tgggcgcaat
     4981 tcgttattga cggcaatgga cagggacgcg ttgcctggcg cttgccggag cagagttcaa
     5041 ccattctggt gctgaataaa gccgggcaaa ttcagtgggc aaaggacggc agcctgacgc
     5101 cggaagaggt agatcatgtc attgcgttgg cgcagaaact gatcaatgaa taaaaaactc
     5161 aaaggattga gggcgttagt gagatgaagc tggttgaaca ctacatcatg cgtggtacgc
     5221 gccgtctggt gctgattatc gtcggttttc tgatctttat tttcgccagc tactccgcac
     5281 agcgttatct gaccgaagcg gcaaatggga cattagctct tgatgttgtg ctggatatcg
     5341 ttttctacaa cgtactgatt gcactggaga tgttgttacc tgttggtctg tatgtgtcag
     5401 ttggcgtgac gctagggcag atgtataccg actcggaaat taccgctatc tctgcggcgg
     5461 ggggcagtcc gggacgcttg tacaaagccg ttctttatct ggcgataccg ctaagtattt
     5521 ttgtcaccct tctgtcgatg tatggtcgtc cgtgggctta tgcgcagatt tatcaactgg
     5581 agcagcagtc gcagtcggag ctggatgttc gccagttgcg ggcaaagaaa tttaacacta
     5641 acgataacgg acgaatgatc ctttcgcaga cggttgatca ggataataat cgcctgactg
     5701 acgcgctgat ttatacttct actgccaatc gaacccgtat tttccgcgcc cgttcggttg
     5761 atgtggttga cccatcaccc gagaaaccga ccgttatgtt gcacaacggc actgcctatc
     5821 ttctcgatca tcaggggcgt gacgacaacg aacagatcta ccgtaatctg caattacatc
     5881 tgaatccgct ggatcaaagc ccgaacgtca aacgcaaagc gaaatcggtc acggagctgg
     5941 cgcgctccgc ctttcctgcc gatcatgccg aactgcaatg gcgacaaagc cgtggcctga
     6001 ccgcattgtt gatggcgctg ctggccattt cattaagtcg ggtaaaaccg cggcaagggc
     6061 gattttcaac gttattgcca ctgacgttgc tgtttattgc cattttttat ggcggcgacg
     6121 tctgccgtac actggtggct aacggcgcga ttcccctcat tcctggtttg tggttagtgc
     6181 ccggactcat gctaatgggc ctgctgatac tggtcgcacg tgacttctct ttgctgcaga
     6241 aattttcccg atgaatgttt tcagtcgcta tttaatccgt catctctttc tcggttttgc
     6301 cgccgctgca gggctattgc tgccgctttt taccaccttc aacctgatta acgaactgga
     6361 tggtgtcagc ccgggcggtt atcgctggac tcaggcggtg ctggtggtgc taatgacctt
     6421 accgccattc gcagcacggg gttttctatc ttccgtattg cactggtggc gctggttgcc
     6481 gggatattgt ggactgtttc gttaggcgcg atagatgagt gggtggcgtc gccattgcag
     6541 caacaggcgt tgcaaatcaa atcgaccgcc accgcgttgg gggaggacga tgacattacc
     6601 ggcaatatgc tgtgggccag gcgtggtaat gaatttgtga cggtgaaatc gctgaacgag
     6661 cagggccagc cggtgggcgt ggagatattt cattatcgcg acgatctctc gcttgaatcc
     6721 tacatttatg cacgcagtgc caccattgaa gacgacaaaa cgtgggtcct gcatggtgtg
     6781 aatcataaaa aatggctgaa tggcaaagaa acgctggaaa cattagataa tcttgcctgg
     6841 caatcggcct tcaccagtat gaatcttgaa gagttatcaa tgccggggaa tactttttct
     6901 gtccgtcagc ttaatcatta catccattat ttgcaggaaa ccgggcaacc cagcagtgaa
     6961 taccgccttg cactgtggga aaaactgggg caacctatcc tgaccctggc gatgattttg
     7021 ctggctgtgc cgtttacctt tagtgccccg cgttcgcctg ggatgggtag ccgtctcgct
     7081 gtaggcgtca tcgtgggctt actcacctgg atcagctatc aaatcatggt taatctggga
     7141 ttgctatttg cgttaagcgc acctgttacc gcgctcggtt taccggtagc gtttgtgttg
     7201 gtggcgttga gcctggtgta ttggtatgac agacaacatt aaacccgccg tggcgcaaac
     7261 accacggcgg gctcacatta ctgcttcaga gtagcaaatg cctgaatgat gacatcgatt
     7321 tgctcacgac tgtgggcagc atttacgcta cagcgcagca gcgtgatgcc tgcaggcgcg
     7381 gcaggcggca ggataagatt cacatacacg cccagcgaga taagctcgcg ccagatgcgt
     7441 aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg tgaacttaac
     7501 tcataaccca gtttttgcag accgtcatac agatggttgg cgttatccat cagcttttgc
     7561 cgcagctctg gttgtgtggc aatagttctt aacgacgagc gcacggtcgc aatgcaggac
     7621 ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac ttccatcgct
     7681 tccgaaccga cagcaaaacc gccaatcgag gccagacttt tactgaaagt accaacgata
     7741 atatccacat catcttcaac gccgacagct tcagccagtc cgcgccccgt tgcgcctaac
     7801 acgccaaacg aatgcgcttc atcgacaatc aaataaccac cgagacgacg tttgatatca
     7861 acaatttccg ccagcggcgc aacgtcaccg agcatgctgt agatgccctc aacgatgata
     7921 atcgcctctt tcgcccgttc accgaggcga accatgcgcc gttccagatc tttagcatcg
     7981 ttatggcgga aacgaataat ttccgcgcca ccgagagaac aggcgtcata aatactggcg
     8041 tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgcgct gatgacgcct
     8101 aaattagcgg tataaccggt tgaaaaaacg atagcggtag ggcgattgaa aaactcggca
     8161 atctctttct ctaacgccag gtgcggcgca taactgccgt ttgccatacg agaaccggtg
     8221 gtgccggtgc cttgggccgc taacgcggcc tgaccttcgc tgatggcatc atgattaaag
     8281 gttaaaccga gataattatt ggtcccggcc agaatcactt ttttattgcc gatccggcct
     8341 tcggtggcgg aatacacttc atcaatacag gtgccaaacg gtgttaaccc gttgtcacta
     8401 aattgcagac gctcgccagc aaggcgcgca tatttatcgt atagccccat tatttattct
     8461 ccagccatgg aagtagagtt tccattaatt gcgcaggggt tttgacatcc agcagaatat
     8521 taataggaat ggaaatatcg aatctgtctt ccagcatcat taagagatcc atcactttga
     8581 tcgactccag gccaagatcg ttgacgagat cgctgtcggg cttaatttcc acaccgtttt
     8641 cgaccaaatc ctgtaaacag gaaaggatgt aatccattac tatttcacga tttaccatag
     8701 aaacgttaac acacctttaa acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc
     8761 aggctaatcc cgggaaacca attaatatct tcagaaatac ggttattact tgccgaccag
     8821 tcggcgtggg ttaattcacg aattttcgag cgggtcagca taggttcttt acctgccagg
     8881 cgactcaacg cggtactgat atccgcaagg cctgtgagta ccggcagagg aatgtcaacc
     8941 attcgcacgg aaccacaacg ggctttggcg gcaagctgtc gtacgcgttg ccagtcatag
     9001 ccaccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc ggcgcttaac
     9061 cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagttg tgtatctggc
     9121 gcaccaagtc gtggcagcag gccgcgtagc atccagtcaa acagcggttt taactcttta
     9181 tcgccgggac catacacggc tgtcgggcga aaaacgccca gcgtaatttc gtccgccatt
     9241 gctgccagtc gttgctcggc gacgtatttg gattttgcat accaggagag ctcgggatgg
     9301 cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc ttttgctgct
     9361 tgcatcaggc gcaggctgcc gtcaacgtta cagcgggtga aaatctcttc tttgtgcccg
     9421 cgcacttgcc cggcgcaatg gaccactacg ctggctccgg caaccagctc gctaagtgaa
     9481 tgagtatctt ccagcgaacc gcgcacccag gtgagattat cattgacgtg agcgcgggca
     9541 gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcgat aatatattta
     9601 ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat tcatgccagg gattcctgca
     9661 cattaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca cgggcaggct
     9721 tgccggagga cgttcgggga atactgtggg gcggcaacag atcgatagcc gcggtcacgc
     9781 caaattcgct ttggatccgt gccgccagcg cgtggataag ctgcccgcga cgttcttcgt
     9841 cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg
     9901 catcgccaga atgaatttcc ggttcctgtt ccgctatata ttcaatatcc tgcggccaga
     9961 tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac agataaccgt
    10021 ccagcagata accgaggtcg ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg
    10081 aaacctggtc gccaaagtaa ccgctcatca gactgggacc ggagatgcaa atatggccta
    10141 ccacacgttc cgcgaccggc atacctgctt cattgcggat ttcaatacca tgttccggca
    10201 acgctttgcc gcagttgacg aaagtcgata cggcgcgtgt ctctgcaccc ggcgcgacgg
    10261 ctttaccctg atattcgagg atgtcgcgat ccacttcgtt aaccacaacc ccggaggctt
    10321 catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc atgaaagttt
    10381 tattgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt tctgcggaga
    10441 tcggctcagc accaatacca gcgacgcgcc agcaggaaag atccagttca gcgagatctt
    10501 tttcattcac gcggcgttgg cacaattcat agccaaacgg cggcgcaacg gaaacggtgc
    10561 cgcgattttt actgatcaat ttaagccatt gcaaaggacg catggcaaaa tcctgagtgc
    10621 gcaaataatc tactgaaagt tgcgtggcga cggggtcagg agaaagccga ccagtcccat
    10681 atcatggtag aaaggctgcc aggagacgca gcggtcgcta gggcgtaatt taatgccatc
    10741 gtggcttata gcacgtagat tagccatcac ttcgcgatgg gggataatga cgccacgggg
    10801 aaaacgggtg ctgccggagg tgtactggag gtaggcgata tcgttcggaa ctggacgctg
    10861 gagcgcaaca tcggcttccg gtaatgcctt aaaccaagcg tggcttaaaa catgtaattc
    10921 ggggttgtta tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag tgataatggc
    10981 tgcgggctgg caacttgcta gtaaaccctg gagtttggcg ctccaggaat cccgctgacc
    11041 gacgcccatt ggaatcgcca acgggacggc gactaagcca gcatactgac aggcaaaaaa
    11101 agcctctaca aacccgctac tcgtttcggc aatcagcgcc acgcgttcgc cttttttcag
    11161 gttcagcgat aacaaccgct tcgcaccagc ttcggcacgc gtttttaatg tctgatattc
    11221 cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc tactcagagc
    11281 ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag gtagggaatg
    11341 cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatcaaaa ataaaatgac
    11401 aataatgcgg tggattatat aggcataaaa gataaatgag atagtatttt catcggtgtg
    11461 aaaatgaaca ttgatttaaa taatgagttt aagttttttg tttttattat gaatgatatt
    11521 taatttatga atatattaag aagaagcctt gtccggtcgc cttaaatcga caaaaggcat
    11581 tgaggcgttt ctgtgtagcc tctgacctta tcataatcat taattaatac tatttattcg
    11641 tcgtttgtat tcattgagtt gattattaag atgccgctcg ttgctgttgc aacctgtttt
    11701 tcaccattaa ttatttgtta tccacagaga tatggaatgc gatctgctga tgattaaaat
    11761 tgaaaaagta attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta
    11821 tcccgacgat ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc
    11881 gaaaaatccg gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca
    11941 gatagttggg cgtattaccg cgcaaataga taccttgcac cgtgagcgtt acggtgaaga
    12001 taccggtcat ttcggcatga ttgacgccat tgatgatccg caggattttg ccgcgttgtt
    12061 tggcgcagcg gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag
    12121 cctgaatatc aatcaggaaa gcggattact gattgaaggt tttgacacac caccctgtgc
    12181 gatgatgcca cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa
    12241 aggcattgat ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct
    12301 aaaaaaactg atggatcagg tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg
    12361 gtttgccgaa gagatgcaga tcctgcgtga gattttcaac tctggctggc agcacaactg
    12421 gggatttgtg ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct
    12481 ggtgccggac gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt
    12541 cggcttgcct aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg
    12601 ctgggcaaaa ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct
    12661 gatgggcgtg cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt
    12721 gattgaagcc ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg
    12781 gatcctcgaa accaatacag gcatgaataa catgctggaa cgcattgggg cggagccgta
    12841 caagcgttac cgattgtatg aaaagcaaat ttaataccag atcctgttta gcgcaggggc
    12901 ggtcattcgg gagagggtat agatgaacag atttaacacc agatcctgtt aagtgcaggg
    12961 gcggtcccct cgccttgggg atagggtaaa actctgctca cattcccacc ttgttcatct
    13021 ccagcggcac cgccgagcga atcaaatggt ggccttccag ctcgatatcg tggagattct
    13081 ttttcacggt ccgtacatga tcgcgcgctg cgcgctgagc gacgtgcggc agccgctgca
    13141 acaccgcgtt gtagatccgc gcatgctggc gatcgatctg ctttttttgc tgtggtcgat
    13201 ggtagagatt atttacgctg gcaaacactg aattaaacat cagatcggtc aatgattgca
    13261 gcgtaaacac cagcacctga ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat
    13321 ccaactgcgc atgttcgatc agcgaaatct ctttgttgtt ctcactggcg gcgagcattt
    13381 tttcataaca gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc
    13441 ttgccgattc gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct
    13501 gcgtactgaa cagatggatc agcgggctgg tgtcctgcac ccgattaagc cgtgcgacac
    13561 gagaatcgcg accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt
    13621 cacgcagtgc ggagcgtgag aagccgagct tttcacacag tcgacgttcc gagggaagcg
    13681 gctgaccgac cttcagtacg ccgtcgataa ttaaccgttc gatactctct gcaaccactt
    13741 cgcaaatagg gcgacgttca tctttcattc ccggacctcg tgcacaggta ggaccaattt
    13801 ttctgttctt aactcgcaaa acacgcacat cacgtaagtg attatgttac atcaatttaa
    13861 cattaagtta accaagacaa ggtcacagag ctggaaaaaa aatggtctga ccggtagcta
    13921 aagagataga cgaaaacgaa aagcccgctt aataactgtt cacagaagca gcgcgcaaaa
    13981 atcagctgcc acacaacaca acaaagcgaa gcctactcat gagcatcttg tacgaagagc
    14041 gtcttgatgg cgctttaccc gatgtcgacc gcacatcggt actgatggca ctgcgtgagc
    14101 atgtccctgg acttgagatc ctgcataccg atgaggagat cattccttac gagtgtgacg
    14161 ggttgagcgc gtatcgcacg cgtccattac tggttgttct gcctaagcaa atggaacagg
    14221 tgacagcgat tctggctgtc tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag
    14281 gcaccgggct ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc
    14341 gctttaaaga gatcctcgac attaaccccg ttggtcgccg cgcgcgcgtg cagccgggcg
    14401 tacgtaacct ggcgatctcc caggccgttg cgccgcataa tctctattac gcgccggatc
    14461 cttcctcaca aatcgcctgt tcaattggcg gcaatgtggc ggaaaatgcc ggtggcgtcc
    14521 actgcctgaa atatggtctg accgtacata acctgctgaa aattgaagtg caaacgctgg
    14581 acggcgaggc gctgacgctg ggatcggacg cgctggattc acctggtttt gacctgctgg
    14641 cgctgttcac cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc
    14701 tgccgaagcc gcccgtggcg cgggttctgt tagccagctt tgactcggta gaaaaagccg
    14761 gacttgcggt tggtgacatc atcgccaatg gcattatccc cggcgggctg gagatgatgg
    14821 ataacctgtc gatccgcgcg gcggaagatt ttattcatgc cggttatccc gtcgacgccg
    14881 aagcgatttt gttatgcgaa ctggacggcg tggagtctga cgtacaggaa gactgcgagc
    14941 gggttaacga catcttgttg aaagcgggcg cgactgacgt ccgtttggca caggacgaag
    15001 cagagcgcgt acgtttctgg gccggtcgca aaaatgcgtt cccggcggta ggacgtatct
    15061 ccccggatta ctactgcatg gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg
    15121 aaggcattgc ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc tttcatgccg
    15181 gagatggcaa catgcacccg ttaatccttt tcgatgccaa cgaacctggt gaatttgccc
    15241 gcgcggaaga gctgggcggg aagatcctcg aactctgcgt tgacgttggc ggcagcatca
    15301 gtggggaaca tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg
    15361 atgaaatcac gaccttccat gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc
    15421 ctgggaaaaa cattcccacg ctacaccgct gtgctgaatt tggtgccatg catgtgcatc
    15481 acggtcattt accttttcct gaactggagc gcttctgatg ctacgcgagt gtgattacag
    15541 tcaggcgctg ctggagcagg tgaatcaggc aattagcgat aaaatgccgc tggtgattca
    15601 gggcagcaat agcaaagcct ttttaggtcg cccagtcacc gggcaaacgc tggatgtgcg
    15661 ttgtcatcgc ggcattgtta attacgaccc gaccgagctg gtgataaccg ctcgtgccgg
    15721 aacgccgctg gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc ttccctgcga
    15781 gccgccgcat tatggtgaag aagccacctg gggcgggatg gtcgcctgcg ggctggcggg
    15841 gccgcgtcgc ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc gcatcattac
    15901 cggcactgga aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg ccggatacga
    15961 tctctcacgg ttaatggccg gaagctacgg ttgtcttggc gtgctcactg aaatctcaat
    16021 gaaagtgtta ccgcgaccgc gcgcctctct gagcctgcgt cgggaaatca gcctgcaaga
    16081 agccatgagt gaaatcgccg agtggcaact ccagccatta cccattagtg gcttatgtta
    16141 cttcgacaat gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa aagcagcgcg
    16201 tgaactgctg ggtggcgaag aggttgcggg tcagttctgg cagcaattgc gtgaacaaca
    16261 actgccgttc ttctcgttac taggtacctt atggcgcatt tcattaccca gcgatgcgcc
    16321 gatgatggat ttacccggcg agcaactgat cgactggggc ggggcgttac gctggctgaa
    16381 atcgacagcc gatgacaatc aaatccatcg catcgcccgc aacgctggcg gtcatgcgac
    16441 ccgctttagt gccggagatg gtggctttgc cccgctatcg gctcctttat tccgctatca
    16501 ccagcagctt aaacagcagc tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc
    16561 ggaactttga ggagcaggct atgcaaaccc gattaaccga agagatgcgg cagaacgcgc
    16621 gcgcgctgga agccgacagc atcctgcgcg cctgtgttca ctgcggattt tgtaccgcaa
    16681 cctgcccaac ctatcagctt ctgggcgatg aactggacgg gccgcgcggg cgcatctatc
    16741 tgattaaaca ggtgctggaa ggcaacgaag tcacgtttaa aacacaggag catctcgatc
    16801 gctgcctcac ttgccgcaat tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt
    16861 tgctggatat cgggcgtgat attgtcgagc agaaagtgaa acgcccactg ccggagcgaa
    16921 tgctgcgcga aggattgcgc caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc
    16981 aggtagggct ggtgctgcga ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg
    17041 aaacggtgaa agctaaaccg cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg
    17101 aaggctgcgc ccagcctacg ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg
    17161 atcgtctggg gatcagcgtc atgtcagcta acgaagcagg ctgttgtggc gcggtggact
    17221 atcatcttaa tgcgcaggag aaagggttgg cacgggcgcg caataatatt gatgcctggt
    17281 ggcccgcgat tgaagcaggt gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt
    17341 tcgtcaaaga gtatgggcag atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc
    17401 aggtcagtga actggcggtc gatttagtcg aacttctgcg cgaggaaccg ctggaaaaac
    17461 tggcaattcg cggcgataaa aaactggcct tccactgccc gtgtacccta caacatgcgc
    17521 aaaagctgaa cggcgaagtg gaaaaagtgt tacttcgtct tggatttacc ttaacggacg
    17581 ttcccgacag ccatctgtgc tgcggttcag cgggaacata tgcgttaacg catcccgatc
    17641 tggcacgcca gctgcgggat aacaaaatga atgcgctgga aagcggcaaa ccggaaatga
    17701 tcgtcaccgc caacattggt tgccagacgc atctggcgag cgccggtcgt acctctgtgc
    17761 gtcactggat tgaaattgta gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa
    17821 gtcattctta gccagcaaat ggcgagtgca attattgccg caggtcagga agaggcgcag
    17881 aaaaataact ggtctgtttc cattgctgtt gccgatgacg gcggtcatct gctggcgtta
    17941 agtcgcatgg acgattgcgc gccgattgcg gcttacatct cccaggagaa agcgcgtacc
    18001 gccgcgctgg ggcgtcgtga aactaagggc tatgaagaga tggtgaacaa cggacgtacc
    18061 gcgttcgtga ctgcgccgtt actgacgtcg ctggaaggcg gcgtaccggt tgttgttgat
    18121 gggcaaatta ttggtgccgt gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc
    18181 gcgaaagcgg cagcagcggt gttggcgaaa taagcgaaaa cgaggagaga aacaatgagt
    18241 caaaccataa cccagggccg tttacgcatt gacgccaatt ttaaacgttt tgtggatgga
    18301 gaagttttgc cgggcgtgga actggacgct gctgcattct ggcacaatgt tgatgagatc
    18361 gttcacgatc tggcaccaga aaatcgtcag ttgctggcag aacgcgatcg cattcaggcg
    18421 gcgcttgatg agtggcatcg cagcaatccg gggccggtaa aagataaagc ggcctataaa
    18481 tctttccttc gtgaactggg ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc
    18541 acgggcattg acagcgaaat caccagtcag gcggggccac agctggtggt tccggcaatg
    18601 aacgcccgct acgcgctgaa cgcggcgaac gctcgctggg gctcactgta cgatgcgtta
    18661 tacggcagcg acatcatccc gcaggaaggg gcgatggtca gtggctacga tccgcagcgc
    18721 ggtgagcagg ttatcgcctg ggttcgacgt ttcctcgatg aatcactacc gctggaaaac
    18781 ggcagctatc aggatgtggt ggcgtttaag gtggtcgata aacaattacg catccagttg
    18841 aaaaatggta aagaaaccac gttacgtact ccagcacagt ttgtcggtta ccgtggcgat
    18901 gccgctgcgc cgacctgcat tttgctgaaa aataacggcc tgcatattga actgcaaatt
    18961 gatgccaacg ggcggattgg caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa
    19021 gctgctatca gtaccattct cgactgcgaa gattcggtcg cggcggttga tgcggaagat
    19081 aaaatcctgc tgtaccgcaa tctgctgggc ctgatgcagg ggactctgca agagaaaatg
    19141 gagaaaaacg gtcggcaaat cgtacgtaaa ctgaatgacg atcgtcagta caccgccgcc
    19201 gatggctctg aaatttctct gcacggacgc tcgttgctgt ttatccgcaa cgtgggtcat
    19261 ttgatgacca ttcctgtgat ttgggacagc gaaggcaatg aaatcccgga aggtattctt
    19321 gatggcgtca tgactggcgc gattgccctc tatgatttaa aagtgcagaa aaactcgcgc
    19381 actggcagcg tctatattgt gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc
    19441 aacaaactgt ttacccgcat tgagacaatg ctcggtatgg caccgaatac cctgaaaatg
    19501 ggcattatgg atgaagaacg ccggacctcg ctgaacttgc gtagctgtat cgctcaggcg
    19561 cgcaaccgcg tggcgttcat caataccggt ttcctcgacc gtaccggcga tgaaatgcat
    19621 tcggtgatgg aagctggccc gatgctgcgt aaaaaccaga tgaaatcgac gccgtggatc
    19681 aaagcctacg aacgtaataa cgtgctttcc ggtctgttct gtgggctgcg cggtaaagcg
    19741 caaatcggta aaggcatgtg ggcaatgccg gacctgatgg cagacatgta cagccagaag
    19801 ggcgaccaac tgcgtgccgg ggcaaacaca gcctgggttc cgtcaccaac cgctgctacg
    19861 ctccatgcgc tgcactacca ccaaaccaac gtacagagcg tgcaagccaa cattgcccag
    19921 accgagttca atgctgaatt tgaaccgctg ctggacgatc tgctgactat tccggtggct
    19981 gaaaacgcta actggtcggc gcaagagatc caacaagagc tggataacaa cgtgcagggg
    20041 attctggggt acgtggtgcg ttgggtggag caggggattg gttgttcaaa agtgccggat
    20101 attcacaacg tggcgctgat ggaagaccgc gcaacgctgc gtatctccag ccagcatatc
    20161 gccaactggt tacgtcacgg tattctgacc aaagaacagg tgcaggcgtc gctggagaat
    20221 atggcgaaag tggttgatca gcaaaacgct ggcgatccgg cttatcgtcc gatggcgggg
    20281 aatttcgcta actcgtgtgc ttttaaagct gccagcgatt taatcttcct cggcgtgaaa
    20341 cagccaaacg gttataccga accgttatta cacgcctggc gtttacgcga aaaagaaagt
    20401 cattaatagc gtcaaaaaag ccccggcaag ttttattgtc ggggctatga tagtaactgc
    20461 ggtagtacat ttatcccgga aataaccgga gcgattatca gggtttaaca atattaaata
    20521 cccgtagc
//
LOCUS       DADLSE010000067        20521 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLSE010000067 DADLSE010000000
VERSION     DADLSE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.067"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN692_002381"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN692_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7264670.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN692_002382"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN692_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7264671.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN692_002383"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN692_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7264672.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN692_002384"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN692_002384"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7264673.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN692_002385"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN692_002385"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7264674.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGREPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="JN692_002386"
     CDS             4449..4814
                     /locus_tag="JN692_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7264675.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="JN692_002387"
     CDS             4964..5482
                     /locus_tag="JN692_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7264676.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="JN692_002388"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="JN692_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="JN692_002389"
     CDS             6713..7195
                     /locus_tag="JN692_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7264677.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN692_002390"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="JN692_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7264678.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN692_002391"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="JN692_002391"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7264679.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN692_002392"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="JN692_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7264680.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN692_002393"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="JN692_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7264681.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN692_002394"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="JN692_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7264682.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10864..11730
                     /locus_tag="JN692_002395"
     CDS             10864..11730
                     /locus_tag="JN692_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7264683.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="JN692_002396"
     CDS             11751..13037
                     /locus_tag="JN692_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7264684.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN692_002397"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="JN692_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7264685.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="JN692_002398"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="JN692_002398"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7264686.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="JN692_002399"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="JN692_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7264687.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="JN692_002400"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="JN692_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7264688.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="JN692_002401"
     CDS             complement(16985..17275)
                     /locus_tag="JN692_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7264689.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN692_002402"
     CDS             complement(17265..17741)
                     /gene="ratA"
                     /locus_tag="JN692_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7264690.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="JN692_002403"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="JN692_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7264691.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="JN692_002404"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="JN692_002404"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="JN692_002405"
     CDS             19137..20378
                     /locus_tag="JN692_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7264692.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5298 a         5357 c         4820 g         5046 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcattaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgctaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacaggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggctcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaagcaatt tggtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcaaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cacccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaatcagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca ttcccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccaa ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcca
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttcgacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgcta
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gagtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagaaacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgcctgtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggatcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg cgggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 t
//
LOCUS       DADLSE010000068        20441 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLSE010000068 DADLSE010000000
VERSION     DADLSE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.068"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..214)
                     /locus_tag="JN692_002406"
     CDS             complement(<1..214)
                     /locus_tag="JN692_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HAY7264693.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKE"
     gene            complement(222..932)
                     /locus_tag="JN692_002407"
     CDS             complement(222..932)
                     /locus_tag="JN692_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7264694.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN692_002408"
     CDS             complement(1148..2971)
                     /gene="typA"
                     /locus_tag="JN692_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7264695.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            3344..4753
                     /gene="glnA"
                     /locus_tag="JN692_002409"
     CDS             3344..4753
                     /gene="glnA"
                     /locus_tag="JN692_002409"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7264696.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEAAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4926..5975
                     /gene="glnL"
                     /locus_tag="JN692_002410"
     CDS             4926..5975
                     /gene="glnL"
                     /locus_tag="JN692_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7264697.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5987..7396
                     /gene="glnG"
                     /locus_tag="JN692_002411"
     CDS             5987..7396
                     /gene="glnG"
                     /locus_tag="JN692_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7264698.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7508..7618
                     /gene="yshB"
                     /locus_tag="JN692_002412"
     CDS             7508..7618
                     /gene="yshB"
                     /locus_tag="JN692_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7264699.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN692_002413"
     CDS             complement(7847..9220)
                     /gene="hemN"
                     /locus_tag="JN692_002413"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7264700.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN692_002414"
     CDS             complement(9409..9918)
                     /gene="yihI"
                     /locus_tag="JN692_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7264701.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10500..11132
                     /locus_tag="JN692_002415"
     CDS             10500..11132
                     /locus_tag="JN692_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7264702.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN692_002416"
     CDS             complement(11514..14300)
                     /gene="polA"
                     /locus_tag="JN692_002416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7264703.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            14302..14544
                     /locus_tag="JN692_002417"
     CDS             14302..14544
                     /locus_tag="JN692_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264704.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFVFHAVKNKSIPNN"
     gene            14664..15596
                     /locus_tag="JN692_002418"
     CDS             14664..15596
                     /locus_tag="JN692_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7264705.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(15637..17067)
                     /locus_tag="JN692_002419"
     CDS             complement(15637..17067)
                     /locus_tag="JN692_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7264706.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKNRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN692_002420"
     CDS             complement(17222..17848)
                     /gene="dsbA"
                     /locus_tag="JN692_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7264707.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN692_002421"
     CDS             complement(17865..18851)
                     /gene="srkA"
                     /locus_tag="JN692_002421"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7264708.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHTGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(18928..19197)
                     /locus_tag="JN692_002422"
     CDS             complement(18928..19197)
                     /locus_tag="JN692_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7264709.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            19267..19851
                     /gene="mobA"
                     /locus_tag="JN692_002423"
     CDS             19267..19851
                     /gene="mobA"
                     /locus_tag="JN692_002423"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7264710.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            19833..20360
                     /gene="mobB"
                     /locus_tag="JN692_002424"
     CDS             19833..20360
                     /gene="mobB"
                     /locus_tag="JN692_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7264711.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         4969 a         5165 c         4951 g         5356 t
ORIGIN      
        1 gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga
       61 cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca
      121 gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt
      181 gtagaatctt tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg
      241 tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg
      301 gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag atacttgctg
      361 atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg
      421 tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga
      481 gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc
      541 cagccggtga tgcgatagag ttcagttgat ggcgtcactg caatcagtgg cgcgagcgtc
      601 tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aagaaggctc tcgcccttgc
      661 tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg
      721 gtcacgaacc accccttacg atccttacgg aagattttgg attctgcctc gaggttcaac
      781 aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa
      841 gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt
      901 gcattttcta cggtggattg attctccgcc attgtttaat ccttttgatt taaaccagat
      961 gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata
     1021 attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg
     1081 tgtgcgctca cgacaggtaa aaaaaacctg ccatcgctgg caggtttttt atgactaaaa
     1141 aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt tccgtcaggt
     1201 gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca tcatcgatga
     1261 actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaaca gcttcgtcag
     1321 taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca gtcaggtcgt
     1381 tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg tgaccgagga
     1441 acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gacagcttta ccctgaccgt
     1501 tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg tcgtcgtagt
     1561 ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac tcagaacgga
     1621 agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct ttaccgtctg
     1681 gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcatcaca gaaccctgat
     1741 gctgttcttc aacgtccagc gtcacgtttt catacggctc ttgtttacga ccgtcgattt
     1801 cacggaagat tactttcgga cgggataccg ccagttcgaa accttcacga cgcatgtttt
     1861 cgataagaac agagaggtgc agttcaccac gaccagaaac gcggaacgca tcggcgtctt
     1921 cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga cgatcgagga
     1981 tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg ttaacgcaga
     2041 agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca acgttttgcg
     2101 tgtcgcaaac ggtgtcagaa atgttcagtt cgcccaggcc cgtgatcgcc acgatatcgc
     2161 cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc actttaccga
     2221 ctttcgcgtt acgggttttg ccttcgctat cgatgatagt aacctgctgg ttcggcttca
     2281 ctttaccacg cttgatgcgg ccaatgccga taacgccaac atagctgttg taatcgagct
     2341 gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga acgtggtcaa
     2401 caatcgcctg atacagcggg gtcatgtctt ccgccatatc ttcgtggtcc agacccgcga
     2461 taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg gtcgcgtcga
     2521 ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg ccagggcggt
     2581 caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt ttggttacga
     2641 agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact gagtctacca
     2701 tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta tcaacgatgt
     2761 tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta atcccacgct
     2821 ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga gagtcgaacg
     2881 taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct acgtgcgcga
     2941 tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta gaaatagcgc
     3001 gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct ccgcaaacaa
     3061 gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta tccaaaggtc
     3121 attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc aacattcaca
     3181 tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg ggcaatttaa
     3241 aagttggcac agatttcgct ttatcttttt tacggcgaca cggccaaaat aattgcagat
     3301 ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg ctgaacacgt
     3361 actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca ccgataccaa
     3421 aggtaaagaa caacacgtca ctatccctgc tcatcaggtg aatgctgaat tcttcgaaga
     3481 aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg aatccgacat
     3541 ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg actccaccct
     3601 gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc gtgacccgcg
     3661 ctctattgcg aagcgcgccg aagattacct gcgttccact ggcattgccg acaccgtact
     3721 gttcgggcca gaacctgaat tcttcctgtt cgatgacatc cgtttcggat catctatctc
     3781 cggttcccac gttgctatcg acgatatcga agccgcatgg aactcctcca cccaatacga
     3841 aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt tacttcccgg ttccgccggt
     3901 agactctgct caggatattc gttctgaaat gtgtctggtg atggaacaga tgggtctggt
     3961 ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg ctacccgctt
     4021 caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgtcg tgcataacgt
     4081 agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg gtgataacgg
     4141 ctccggtatg cactgccaca tgtctctgtc taaaaacggc gttaacctgt tcgcaggcga
     4201 caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa tcaaacacgc
     4261 taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc tggtcccggg
     4321 ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac cgttctgcgt ctatccgtat
     4381 tccggtggtt tcttctccga aagcacgtcg tatcgaagta cgtttcccgg atccggcagc
     4441 taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta tcaagaacaa
     4501 gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag aagaagcgaa
     4561 agagatccca caggttgcag gctctctgga agaagcactg aacgaactgg atctggaccg
     4621 cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt acatcgctct
     4681 gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt ttgagctgta
     4741 ctacagcgtc taatagttga agttgtacta cccggcgcac aacgccggga tttagttgcc
     4801 gtggaaactt tcagcccatc tctgcatggg cttttttctc cgtcaattct ctgatgcttc
     4861 gcgcttttta tccgtaaaaa gctataatgc actaaaatgg tgcaacctgt tcaggagact
     4921 gctttatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca
     4981 gtattttgtt aattgatgac aatctggcga tccattacgc caaccccgcc gcgcaacaac
     5041 tgctcgccca aagctcccgc aaattgtttg gtacgccgtt accggaactg ttgagctact
     5101 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggc tttaccgata
     5161 acgaagtgac gctggtcatc gatggtcgct cgcatatcct ttctgtgacg gcccagcgta
     5221 tgccggacgg catgatcctg ctggagatgg caccgatgga taaccagcgc cgcttaagcc
     5281 aggaacaact acaacatgct caacaagtag ctgcccgtga tttagtgcgc ggcctggcac
     5341 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagttactc agcaaagcgt
     5401 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc
     5461 gaaatttggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta
     5521 ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc
     5581 ggttgattcg tgattacgat cccagcctgc cggaactggc gcacgacccg gatcaaattg
     5641 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gaaggcggtg
     5701 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc
     5761 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata
     5821 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg
     5881 cccgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccaggtcata
     5941 ccgagttctc ggtttacctg cctatcagga aataaaggtg acgtttatgc aacgagggat
     6001 agtctgggta gtcgatgacg atagttccat ccgttgggtg cttgaacgtg cgctcgctgg
     6061 agcgggttta acctgtacga catttgagaa cggcgcggaa gtactggagg cgctggcgag
     6121 caaaacgccg gatgtgctgc tttcagatat ccgtatgccg ggaatggacg ggctggcgct
     6181 gctcaagcag attaaacagc gccatccaat gcttccggtc atcattatga ccgcacattc
     6241 cgatctggat gctgccgtca gcgcctatca acaaggggcg tttgattatc tgcccaaacc
     6301 gtttgatatc gacgaagcag tggcgctggt tgagcgcgct atcagtcatt accaggaaca
     6361 gcagcagccg cgtaatattc agcttaacgg cccaacgacc gatatcatcg gcgaagcgca
     6421 agccatgcag gacgtgttcc gtattatcgg tcggctttcg cgttcttcta ttagcgtgct
     6481 gattaacggc gaatccggca ccggtaaaga actggtcgct catgccctgc atcgccacag
     6541 tccgcgcacc aaagcgccgt ttatcgcgct gaatatggca gctatcccaa aagatttgat
     6601 cgaatcagaa ctgtttggcc acgagaaagg cgcgtttact ggcgcgaata ccattcgtca
     6661 ggggcgtttt gaacaggccg atggcggcac gttatttctc gacgaaattg gtgatatgcc
     6721 gctggatgtg cagacgcgtt tgctgcgcgt gctggcagac ggtcagtttt atcgcgttgg
     6781 cggctatgcg ccggtgaaag tggatgtgcg gattatcgct gccactcacc agaatcttga
     6841 acagcgggtg caggaaggta agttccgtga ggatctgttc caccgcctga acgttatccg
     6901 cgttcatctg ccgccgctgc gcgaacgtcg ggaagatatt ccccgtctgg cgcgccattt
     6961 tttacaggtt gccgcgcgcg aactgggcgt agaagcgaag ttgctgcatc cggaaaccga
     7021 agctgctctg acgcgtctgg cgtggccagg caacgtgcgc cagctggaaa acacctgccg
     7081 ctggctaacg gtgatggccg ccgggcagga agtgttgatt caggatttgc ccggcgaact
     7141 gtttgaatca acggttgcgg agagtacttc gcaaatgcaa ccggacagtt gggcgacact
     7201 tttagcgcag tgggcagaca gagcgctgcg ttccggtcat caaaatctgc tttccgaagc
     7261 gcagccagag ctggagcgga cgttactgac gaccgcgttg cgacatacgc aggggcataa
     7321 acaggaagcg gcgcggctac tcggctgggg ccgcaacact ctgacgcgta agttaaaaga
     7381 gctggggatg gagtgattca cggcttgtgt gtaaagattg attattgagc gcaaattgct
     7441 ggtattttgc gctttactgt tccgataagt tcagtatgat cttgcccgga aaacggggag
     7501 agtcattatg ctggaatcaa taattaatct ggtatcgagt ggcgcagttg acagccacac
     7561 accgcaaact gccgttgctg ccgtgctgtg tgccgcgatg attgggctgt ttagctgaag
     7621 ctcgttgttt atgccggatg cggagaaagt cgtaggcctg ataagacgcg accagcgtcg
     7681 catcaggcat catgctcaga tgcctgatgc ggcgtaaacg ccttatccgg cctacaaaag
     7741 caataatttc aacaggtttt gttgcccagc gcatcaggca atcttgttct cagcacattg
     7801 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactctttaa atcacccgtg
     7861 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
     7921 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
     7981 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
     8041 aatcccactg tttctcaaga ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
     8101 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
     8161 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
     8221 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
     8281 tgtagccctg gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
     8341 caggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
     8401 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
     8461 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaaaacgctc agacgatcgg
     8521 ggttcagctc cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca
     8581 ggccgtaaat caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca
     8641 gtgcaaagat gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
     8701 agtcctgcac gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat
     8761 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggta ttgaaatgga
     8821 agttttcgcg cagcagcttc atcagacggc tgatttgcgc tttattcaga tacgtcggcg
     8881 ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga
     8941 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
     9001 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
     9061 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
     9121 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
     9181 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
     9241 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
     9301 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
     9361 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
     9421 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
     9481 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
     9541 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
     9601 ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
     9661 cggtttgtgc tgtttggtga ctttttcagt cactcccaat ggaatagggg ttttactgcc
     9721 aatacgtgga tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc
     9781 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttacggt cacgagcttc
     9841 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
     9901 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
     9961 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    10021 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    10081 tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    10141 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    10201 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    10261 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg
    10321 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    10381 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    10441 ccttttatta tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt
    10501 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    10561 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    10621 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    10681 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg
    10741 ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg
    10801 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    10861 cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc
    10921 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    10981 tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt
    11041 tgaagaaaca aggcgtagac aagctgcggc agaaactgga tacctggttt agcgagatgc
    11101 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    11161 gtggcgtatc cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat
    11221 agcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    11281 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    11341 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    11401 aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    11461 acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc
    11521 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    11581 cagttttcca tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact
    11641 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    11701 aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    11761 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata
    11821 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    11881 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    11941 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    12001 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt
    12061 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    12121 aatgcggtca gcaagccttt atcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    12181 gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    12241 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    12301 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    12361 atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg ccagaccacg atactccaga
    12421 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    12481 ggcgcgccac ccggcgtttt cttcagcggt ttaataccct gtttttcaaa cagaatggtt
    12541 tgcaactgct tggtggaaga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    12601 tccagctcag ccagacgcaa ggtgagctct tcagaatgat tgtgcagcac tttcggatcg
    12661 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc
    12721 tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt cagatgcaac
    12781 tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    12841 ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat ggttttgtgc
    12901 ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg
    12961 taggactcca gcatggtatc aaacgcaatc ccacgcagtt caatgccgta attcgccaga
    13021 ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc
    13081 ggttttagca actcgagtgc acgctcgtga gagatttgat cgggcgcatc aagataatca
    13141 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    13201 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    13261 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatagtgac gtagttgtca
    13321 taagaaatca ccgttgccgt cacttctggc gcttcatctg caacactggt ttcctgcggc
    13381 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    13441 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    13501 acttccagtt gttcacaggt cagctccagt tcaacatcgg ttttaatcgt cgccaactgg
    13561 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    13621 cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga
    13681 ccttgcagca atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatca
    13741 gaggagtcac ccatcagcgc caggaagtcg atgatcaatt ctggcggcac gccgtactta
    13801 ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag cgtaatattt
    13861 ggcgtcacca gctgcgccat atctttatcg ccagtgctaa tcagcaccgg acgcccggct
    13921 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    13981 agcagcggca gtcccatcgc tttaaccatc gcgtgcagag gttcgatttg tgctcgcaga
    14041 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    14101 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    14161 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    14221 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    14281 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    14341 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    14401 gctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    14461 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa
    14521 aataaatcca tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgaa
    14581 ttatttttct ccttaaggat catccgttat ttgggtcatt ttctgctaaa atctgcgcct
    14641 tgcgcttctt tagtgcactg tctatggcta accttttgaa taaattcatt atgacgagaa
    14701 tactcgctgc gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt
    14761 gttccgtccc gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt
    14821 ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg
    14881 tattactgca tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta
    14941 agaagaactg gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt
    15001 gcgtgctgtt tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct
    15061 gggtgccatt tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt
    15121 cccgcgctta tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc
    15181 gttcttgtga aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc
    15241 gtttcacgca agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa
    15301 aggcggcagg cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta
    15361 atgttacgct gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta
    15421 aactgacgcg gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg
    15481 actacatcaa tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc
    15541 aggaaaaaga ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt
    15601 tactcgcctc tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg
    15661 agacataaat tcttcttcgc tcatctcctg tccgttaaaa acaactttac ccggtgtata
    15721 acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacctaa
    15781 tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc
    15841 ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc
    15901 aaaattgagc gatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat
    15961 actgatatcc agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt
    16021 aatggtcggc tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac
    16081 ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt gcagcaagct gcttctgcat
    16141 cgcaatgtta tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat
    16201 caatgaccac tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta
    16261 ggtcagattg acattgatat aatctttgtc tttaacgcga tccagcctgt tttttgcaac
    16321 gaaagcatcg atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt
    16381 cgactcgctt tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt
    16441 aaaacttttt gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt
    16501 gccttcaata tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc
    16561 accaaaagaa aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc
    16621 accaaacgcg aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag
    16681 cggttgtgtc agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac
    16741 attaccatgc attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac
    16801 aactttctgg cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat
    16861 ttgaaaacgg gaattaaaaa agcctttgtc aaagttttta taacttaacg tcatatcgta
    16921 ggcatgttca cctttctttt tcgcatcgtt aaaatcttta ataaactttt cgataccagg
    16981 ctgaatttgc gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat
    17041 aacacctgta gctgacttac gtatcatata aatattccct gataaaacag caaaactttg
    17101 aatatccact tatgctgaaa taatgtccag cggcaggatg cattatcaga atgtatttat
    17161 atttattaaa taataaaaaa agcccgtgaa tattcacggg ctttatgtaa tttacattga
    17221 attatttttt ctcggacaga tatttcaccg tatcagcata ctgctgaaca aaaacatcca
    17281 tattgctggt atccataccc tgcggattca gctgatattt accgttaaca aacatcgccg
    17341 gaacaccacg caattgcaca tcagctgcgg ctttttcctg ctgagcgacc agagatttca
    17401 ccacgaagct gttccacgcc gcgtcgtact cttcaccttt aatacctgca ttgataaata
    17461 catcgcggat atcagatgct gaacgaatgg tctgggtttt ctgtacgcct tcaaacagcg
    17521 gaacagtcac tttgtcttcc acgcccagcg ccatcgccac agcccatgcc tgagtcagat
    17581 ctttgcccag gtcaccaccc atgaagttga cgtggtattt agtcatcttc acgccttccg
    17641 gcagtttttt cttcacatta tcagaaatat gcagaacttc ttcaaactga tagcagtgcg
    17701 ggcagaagaa agagaaaaac tccagcactt gcggcgcgcc agctaccggt ttttccaggg
    17761 tagtgtactg tttaccatct tcatactgcg ccgccgatgc gctaaacgct aaaactaaac
    17821 cagccagcgc cagccaaatc tttttcatga tctactctct ccgattaata cataggtgtt
    17881 aattgcaaag ggggttcttg tagaactttt gcctgttcta taaaagtcgc cgtctgtcgt
    17941 agccagtaat cttccccggt taaccacggg aaatttttcg ggaacgcggg atcagcccaa
    18001 tgccgcatta gccaggcaag ataataaacc aaacgcatgg cgcgtaaagg ttcaatcagt
    18061 ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag cttcaataat agtttccagt
    18121 tgcatccgct gctcggcttt atcgccattg agcaacatcc acaaatcctg aatagctgga
    18181 ccattacgtg catcatccag atcaacaaac attggaccat cgcgccagag aatattcccg
    18241 gtgtggcagt ctccatgtag ccgcagaacg gtgaaatctt cccgccagtg tgctgtaacg
    18301 gcggcgatca gctcatctgt cgctttcaga aatgccgctt tcaacccgga aggtatcagt
    18361 gtagcgtcct caaacagctt gcgaggctca atgagatatt cattcagacc gatggtcggg
    18421 cgatgaataa aaagctgttt gcgccccgtc tggtgcatac gccctaaata acgcccaacc
    18481 gcttccatct gatcgatatt atcagcttcg aactggcgac caccgacgct tggaaaaaca
    18541 gcgaaataaa atccctgatg attcaataaa gtctgaccgt taaaggccac aggtgctgcg
    18601 accggaactt catcatttac cagctgcaac gcaaattgat gttcttcgag gatttgatcg
    18661 gctgtccaac gttcagggcg ataaaatttg acgacaaaac gtcgacgctc ttcgtcctga
    18721 aattgataga cacggttttc atagctgtta agcggggtaa gaccggaatc cacccggatc
    18781 ccttgctcaa acagagcgtc catgatggta tccgggtgta gtgtctggaa agtaaaagcg
    18841 ctgttgttca tcccatcatc cggaaaacat tacgaatgat gaaggatatc atttcacgat
    18901 gattttggtg gcgccgctta caagctttta ctctttaatt acgccgcgag cgcgtaacag
    18961 cgccgttttg aagtcttctt cataatcttt ctgcaaaccc gggatcaccg catctttcgc
    19021 ggaatcacgc attttcaggt gatagatcag aatgtcatcc gtcaaatccg ccagttcgcc
    19081 gtcaaaacct gactctttcg ccagtttctg caaaaattgc aacaagttaa agtctggctc
    19141 tttttgccag gctggctgga ggagttcaat aacttcattc agacgtttac atttcatagt
    19201 aatgctcctt actcttgaga cagacacgtt agcagggtca atcccacaat aaaagaggcg
    19261 atatcggtga atctgatgac gacgataaca ggcgttgtgc tggcaggcgg taaagccaga
    19321 cgaatgggcg gcgtagataa aggattgctg gacttaaatg gcaaaccgct gtggcaacat
    19381 gtcgctgacg cgcttaagac gcagctctct cacgtcgtgg ttaatgctaa tcgtcatcag
    19441 gaaatctatc aggcaggcgg tctgaaagtg attgaagatt cactggcgga ttacccaggc
    19501 cctctggcag gaatgctttc agtaatgcag caggaagctg gtgagtggtt tttgttttgt
    19561 ccgtgcgata cgccttacat tccccatgat ttagccgccc ggcttaatca tcagcgcaaa
    19621 gatgcgcctg tcgtgtgggt ccatgacggt gaacgcgatc acccgactat tgctctggta
    19681 aaccgcgcta ttgagccttt attactggaa tatctgcaag caggagaacg ccgggtaatg
    19741 gcatttatgc gtctggctgg cggtcatgcg gttgatttca gcgatcataa agatgcattt
    19801 gttaacgtga atacgccaga ggagcttgcc cgatggcagg aaaaacgatg ataccgttac
    19861 tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct tttgaaaaaa ctgatcccgg
    19921 cattatgcgc cagagggatc cgtccggggc tgattaagca tacgcaccat gatatggatg
    19981 ttgataagcc aggcaaagat agctatgagc tgcgcaaggc tggcgcggca caaaccatcg
    20041 ttgccagcca gcaacgatgg gccttgatga cggaaacacc agacgaagaa gagctggatc
    20101 tacattttct cgcaagtcga atggatacct caaagctgga tttgattctg gtcgaagggt
    20161 ttaagcatga agagatcgca aagattgtgc tgtttcgcga tggagccgga catcgacctg
    20221 aagaattagt gatagacagg catgttattg ctgtagccag tgatgtgccg cttaatcttg
    20281 atgtcgcatt actggatatt aatgatgttg aggggctggc tgattttgtg gtggagtgga
    20341 tgcaaaagca gaacggataa gcaagagtag gctggatcag acgcagccag tgtcgcatcc
    20401 gacctttttc tgcaccagcg caaaaaggcc cgactgggcc t
//
LOCUS       DADLSE010000069        20374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLSE010000069 DADLSE010000000
VERSION     DADLSE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.069"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(178..2499)
                     /locus_tag="JN692_002425"
     CDS             complement(178..2499)
                     /locus_tag="JN692_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7264712.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     GTERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLVPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN692_002426"
     CDS             complement(2596..3108)
                     /gene="greB"
                     /locus_tag="JN692_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7264713.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            3300..4019
                     /gene="ompR"
                     /locus_tag="JN692_002427"
     CDS             3300..4019
                     /gene="ompR"
                     /locus_tag="JN692_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7264714.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4016..5368
                     /gene="envZ"
                     /locus_tag="JN692_002428"
     CDS             4016..5368
                     /gene="envZ"
                     /locus_tag="JN692_002428"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7264715.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN692_002429"
     CDS             complement(5449..7071)
                     /gene="pckA"
                     /locus_tag="JN692_002429"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7264716.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7450..9174
                     /locus_tag="JN692_002430"
     CDS             7450..9174
                     /locus_tag="JN692_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7264717.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWALWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN692_002431"
     CDS             complement(9309..10187)
                     /gene="hslO"
                     /locus_tag="JN692_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7264718.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDSKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN692_002432"
     CDS             complement(10212..10613)
                     /gene="hslR"
                     /locus_tag="JN692_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7264719.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN692_002433"
     CDS             complement(10624..11292)
                     /gene="yrfG"
                     /locus_tag="JN692_002433"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7264720.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN692_002434"
     CDS             complement(11357..13492)
                     /gene="igaA"
                     /locus_tag="JN692_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7264721.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPSPTGASAAPLSLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATGVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13812..14372
                     /gene="nudE"
                     /locus_tag="JN692_002435"
     CDS             13812..14372
                     /gene="nudE"
                     /locus_tag="JN692_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7264722.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN692_002436"
     CDS             complement(14537..17089)
                     /gene="mrcA"
                     /locus_tag="JN692_002436"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7264723.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITHKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLGQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17209..17988
                     /gene="hofM"
                     /locus_tag="JN692_002437"
     CDS             17209..17988
                     /gene="hofM"
                     /locus_tag="JN692_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7264724.1"
                     /translation="MAFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            17988..18527
                     /locus_tag="JN692_002438"
     CDS             17988..18527
                     /locus_tag="JN692_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7264725.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18511..18951
                     /gene="hofO"
                     /locus_tag="JN692_002439"
     CDS             18511..18951
                     /gene="hofO"
                     /locus_tag="JN692_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7264726.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18941..19345
                     /gene="hofP"
                     /locus_tag="JN692_002440"
     CDS             18941..19345
                     /gene="hofP"
                     /locus_tag="JN692_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7264727.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNYVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN692_002441"
     CDS             19257..>20374
                     /gene="hofQ"
                     /locus_tag="JN692_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7264728.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRDLGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQ"
BASE COUNT         4614 a         5284 c         5538 g         4938 t
ORIGIN      
        1 ttaattaagg tttcagttgt taatgaagtg ataaatatta acggagtata ttgtgagttt
       61 ggctgtggaa taaaagtgcc ggagatattc tccggcactt ttatgtcagg caaagcgttc
      121 gttgccagat gcggcgtgaa cgccttatcg cgcaggttta ggggccagtc cgggcgttta
      181 acgtttttta cccatcgctg ccgccagcgc atccatcatc gcgctattac cggcaggctg
      241 cgcttcacga ccgcgtggtt tggctgccgg gcggttgttt tgcgggcgtt cattaccgcc
      301 gccgcgacga gagttggttt cgccaggctg ctcgtccaga cgcatagtca gggcgatacg
      361 tttacgctga agatccactt ccagcacttt caccttcaca atgtcgcccg ctttcaccac
      421 ggtatgcgga tcttccacaa acttgttcga caatgaagag atgtgaacca ggccgtcctg
      481 atgcacgcca atatcgacaa acgcgccaaa gttggtgacg ttggtcactg cgccttcgag
      541 gatcataccc ggttgcaggt cgttcattgt ctcgacgcca tcggcaaact gagcggtttt
      601 aaattccgga cgcggatcgc gacccggttt ttccagctct ttgatgatgt cagttactgt
      661 cggcacaccg aatttttcat cagtaaagtc agacgctttc aggttacgca gttcgctgct
      721 gttacccatc agatctttca gtgcctgctg tgttgctgcc agaatgcgtt ccaccaccgg
      781 ataggcttcc ggatgaacgg tagaggcgtc cagcgggtta tcaccgtggt taatgcgcag
      841 gaagcccgcg cactgctcga aggctttcgg caccagacgg ctgactttca gcagttgctg
      901 acggttctgg aactggccgt tctcatcgcg ccaggccacg atgttttgcg ccatcatgcg
      961 cgtcaggccc gccacgcggg ttaatagcgg aacagaagcg gtgttgagat cgacgccaac
     1021 ggcgtttacg cagtcttcta ctactgcatc cagtttgcgg gccagttgcg tctggctgac
     1081 gtcatgctga tactgaccta cgccgataga tttcggatcg attttcacca gctccgccag
     1141 cggatcctgc aaacggcggg cgatagatac cgcaccacgc agcgaaacgt cgagatccgg
     1201 gaactcctgc gctgccagct cggaagctga gtaaaccgac gcgccagctt cgctgacgat
     1261 cactttctgt gcggtcactt tcgggaactg cttctgcacg tcgagataga aacgttcagt
     1321 tccgcgggaa gctgtaccgt tgccgatcgc caccagttca acgttatgtt tttcacacaa
     1381 ggcagcaacg gtcatcgctg cttttgcggc ttgtccggtg tgcgggtaaa tggtatcggt
     1441 cgccaccagt ttgccagtgg catcgaccac cgccactttt accccggtac gcagacccgg
     1501 atcgaggccc atcgttgcac gcagtccggc aggggccgcc atcagcagat cgtgcaggtt
     1561 acgggcaaag acgttgattg cttcatcttc cgcgcgttcg cgcacggtac ccatcagttc
     1621 ggtttccaga tgcatcagca ccttgatgcg ccaggtccag ctcactacgc ctttgcgcca
     1681 gctatccgcc ggggcattgt tcaggcgcag gccaaggtga tccatgatga tttgctcgca
     1741 atagctctct ttgggcggct catcgaactg cggatcggca ttcagcgaaa gctggagtac
     1801 gccttcgtta cgcccacgga acatcgccag cgcgcggtga gaaggcaccg tggacaacgg
     1861 ttcgtgatga tcgaaatagt cgcggaattt cgccccttcc tcttctttac cgctcaccac
     1921 cgtagaaacc aaatgcgcgt tcttccacag ataatcacgc actttcgcca gcagcgcggc
     1981 atcttcggca aaccgttcca tcaggatata gcgcgcgcca tccagcgcgg ctttggtatc
     2041 tgccacgcct ttatcggcat caacatattg tgcagcggcg acttctggcg tgtgtgacgg
     2101 atcgctccac agcaggtcag ccaacggctc aagccctgct tcaatggcga tttgcccgcg
     2161 ggtgcggcgt ttaggtttgt agggcaggta gaggtcttcg agttcggttt tgcttagggt
     2221 ggcgttgatg gccttcgcca gatcatcggt gagtttgcct tgctcggaaa tggatttgag
     2281 gatcgcctga cgtctctctt ccagctcgcg cagatagctc agacgcgttt ccagattacg
     2341 cagctgcgtg tcatccagac cgccggtgat ttccttacga taacgtgcga taaacggcac
     2401 ggtattccct tcgtcaagca ggcgaacggc agcgtcaacc tgttccgggc gcgcctgaat
     2461 ttcacccgca ataatgcggc agaacgaatc attcatcatg gtttgagttc atcttttcgg
     2521 atcaaaaatc aggggatagt tatacggact ggctggcaaa aatgccagcc atcggcagga
     2581 ggttaagact cttccttacg gtttcacgta ctcgatagca ttaacatacc agttcgcttc
     2641 cccggcaggg gtattcacca ccgccagatc gccgacttct tttttcaaca atgcgcgggc
     2701 catcggggaa tcgatagaga tgtaatcttt acggccaaaa atttcatcgt agccgacaat
     2761 acggaaacgg tgagtcacgc catcgtcgtt ttcaatctcc acccacgcgc caaaaaagac
     2821 tttgccttcc tgctgagggg agtaatcgac gattttgaga ttttccaggc atttagtgag
     2881 atagcgcacg cgacggtcga tttcacgcag acgcttttta ttatactgat agtcagcatt
     2941 ttcgctgcgg tcgcccagac ttgcggccca ggtcaccttt tttgtgacct ccgggcgttc
     3001 ttcacgccag agataattaa gctcttgttt gagtttttca tacccttccc gggtaaccag
     3061 gggcgttttc atctcgttga ttccctttgt ctgtttgata atgcgcacat tgggtataac
     3121 gtgatcatat caacagaatc aataatgttt cgccgaataa attgtatact taagctgctg
     3181 tttaatatgc tttgtaacaa tttaggctga aattcatacc agatttagct ggtgacgaac
     3241 gtgagctttt ttaagaatac acgcttacaa attgttgcga acctttggga gtacaaacaa
     3301 tgcaagagaa ctacaagatt ctggtggtcg atgacgacat gcgcctgcgt gcgctgctgg
     3361 aacgttatct caccgaacaa ggcttccagg ttcgaagcgt cgctaatgca gaacagatgg
     3421 atcgcctgct gactcgtgaa tctttccatc ttatggtact ggatttaatg ttacctggtg
     3481 aagatggctt gtcgatttgc cgacgtcttc gtagtcagag caacccgatg ccgatcatta
     3541 tggtgacggc gaaaggggaa gaagtggacc gtatcgtagg cctggagatt ggcgctgacg
     3601 actacattcc aaaaccgttt aacccgcgtg aactgctggc ccgtattcgt gcggtgctgc
     3661 gtcgtcaggc gaacgaactg ccaggcgcac cgtcacagga agaggcggta attgctttcg
     3721 gtaagttcaa acttaacctc ggtacgcgcg aaatgttccg cgaagatgag ccgatgccgc
     3781 tcaccagcgg tgagtttgcg gtactgaagg cactggtcag ccatccgcgt gagccgctct
     3841 cccgcgataa gctgatgaac cttgcccgtg gtcgtgaata ttccgcaatg gaacgctcca
     3901 tcgacgtgca gatttcgcgt ctgcgccgca tggtagaaga agatccagcg catccgcgtt
     3961 acattcagac cgtttggggc ctgggctacg tctttgtacc ggacggctct aaagcatgag
     4021 gcgattgcgc ttctcgccac gaagttcatt tgcccgtacg ttattgctca tcgtcacctt
     4081 gctgttcgcc agcctggtga cgacttatct ggtggtgctg aacttcgcga ttttgccgag
     4141 cctccagcag tttaataaag tcctcgcgta cgaagtgcgt atgttgatga ccgacaaact
     4201 gcaactggag gacggcacgc agttggttgt gcctcccgct ttccgtcggg agatctaccg
     4261 tgagctgggg atctctctct actccaacga ggctgccgaa gaggcaggtc tgcgttgggc
     4321 gcaacactat gaattcttaa gccatcagat ggcacagcaa ctgggcggcc cgacggaagt
     4381 gcgcgttgag gtcaacaaaa gttcgcctgt cgtctggctg aaaacctggc tgtcgcccaa
     4441 tatctgggta cgcgtgccgc tgaccgaaat tcatcagggc gatttctctc cgctgttccg
     4501 ctatacgctg gcgattatgc tattggcgat aggcggggcg tggctgttta ttcgtatcca
     4561 gaaccgaccg ttggtcgatc tcgaacacgc agccttgcag gttggtaaag ggattattcc
     4621 gccgccgctg cgtgaatatg gcgcttcgga ggtgcgttcc gttacccgtg cctttaacca
     4681 tatggcggct ggtgttaagc aactggcgga tgaccgcacg ctgctgatgg cgggggtaag
     4741 tcacgacttg cgcacgccgc tgacgcgtat tcgcctggcg actgagatga tgagcgagca
     4801 ggatggctat ctggcagaat cgatcaataa agatatcgaa gagtgcaacg ccatcattga
     4861 gcagtttatc gactacctgc gcaccgggca ggagatgccg atggaaatgg cggatcttaa
     4921 tgcagtgctc ggtgaggtga ttgctgccga aagtggctat gagcgggaaa ttgaaaccgc
     4981 gctttacccc ggcagcattg aagtgaaaat gcacccgctg tcgatcaaac gcgcggtggc
     5041 gaatatggtg gtcaacgccg cccgttatgg caatggctgg atcaaagtca gcagcggaac
     5101 ggagccgaat cgcgcctggt tccaggtgga agatgacggt ccgggaattg cgccggaaca
     5161 acgtaagcac ctgttccagc cgtttgtccg cggcgacagt gcgcgcacca ttagcggcac
     5221 gggattaggg ctggcaattg tgcagcgtat cgtggataac cataacggga tgctggagct
     5281 tggcaccagc gagcggggcg ggctttccat tcgcgcctgg ctgccagtgc cggtaacgcg
     5341 ggcgcagggc acgacaaaag aagggtaaat aaacgggagg cgaaggtgcc tcccgtttcg
     5401 ttttgctttc tataagatac tggatagata ttctccagct tcaaatcatt acagtttcgg
     5461 accagccgct accagcgcgg cacccgcagg ggtgtcggtg tatttatcga agttgtcgat
     5521 aaacagtttc gccagggttt cggctttttc ctgccactgt tccggagaag cgtaggtgtt
     5581 acgcggatcg agaatcttcg tgtctacgcc cggcagttcg gttgggatcg ccaggttaaa
     5641 catcggcaga gtgaaggttt ctgcattatc cagcgaaccg ttgaggatgg cgtcgataat
     5701 ggcgcgggta tctttaatcg agatacgttt gccagtgccg ttccagccag tgttaaccag
     5761 ataagcctgc gcgcccgccg cctgcatacg tttcaccagc acttctgcgt actgagtcgg
     5821 gtgcagcgac aggaacgccg cgccgaagca agcggagaag gttggcgtcg gttcggtgat
     5881 gccacgctca gtaccggcca gtttggcggt gaagccagag aggaagtgat actgggtttg
     5941 atcggcagtc aggcgagaaa ccggcggcaa cacgccgaaa gcatcagcag tcaggaagat
     6001 aaccttagtc gcgtggcccg ctttggaaac cggcttaacg atgttatcga tgtgatagat
     6061 tggataagaa acgcgggtgt tctcggtttt tgaaccatca tcaaagtcga tagtgccatc
     6121 ttcacgcacg gtgacgtttt ccagcagtgc atcacgacgg atagcgttgt agatttcagg
     6181 ttccgcttct ttcgacagct tgatagtttt tgcgtagcag ccgccttcga aattaaacac
     6241 gccgtcatcg tcccagccgt gttcgtcatc gccaatcagg cggcgtttcg ggtcggtgga
     6301 aagggtggtt ttaccggtgc cggaaaggcc gaagaacacc gcaacatcgc ctttctcgcc
     6361 aacgttggcg gagcagtgca tagaagcgat acctttcagc ggcagcaggt agttcatcat
     6421 cgagaacatc cctttcttca tttcgccgcc gtaccaggtg ccgccaatca gctgcatgcg
     6481 ctcggtcagg ttaaacgcca cgaagttttc ggagttcaga ccctgttctt tccactgcgg
     6541 gttagtgcac ttcgcgccgt tcataacgat aaagtctggt ttgaaacctg ccagctcttc
     6601 atcgctcggg cgaataaaca tgtttttgac aaaatgcgcc tgccaggcca cttcggtgat
     6661 gaaacggacg gaaagacgag tatccgggtt cgcaccacag aaagcgtcga caacgaacag
     6721 acgtttgccg gaaagctgcc tggtcaccag gcctttcaga tgctgccagg tttccggaga
     6781 gagaggtttg ttgtcgttct tacctttgcc tttgtctgcc caccagaaag tatcgcgagt
     6841 ggtatcgtca cggacgatat acttatcttt tggtgaacga ccggtgaaga tcccggtatc
     6901 gacggcaacg gcacccagat tagttaacac cccgcgctca taacctgtca ggctcggatc
     6961 gagctcttcc tgatacagca ggtcgtagct tgggttgtaa acgatatcat gtacgtcact
     7021 gataccataa gcctcgagtt cttgcggggt caaaccattg ttaacgcgca tttcactgct
     7081 ccttagccaa tatgtattgc ctgaatagta aagtcttttt gggggtgtta accgcgacaa
     7141 ggctcataga tttacgtatc tggagaaatt catatttgac aaaaaactcc gtgattcctg
     7201 tcacgaaacg gttgctatta tcgcagaaat agcattttag gtgtggaaaa tgttctcaaa
     7261 aggttaaatc ttggtttata tggcgggaat attgtgagtg gaatcgcatt cctgaatgaa
     7321 attgatgcaa attcaaccgg taaagtatcg attcaccacg ctaacttttt ttgattattt
     7381 tctgaaaaaa gcaaaatact actgcccgga tgcgataatt aacccacctt ttgattctgg
     7441 ataaccaaca tggataacgt tgaactttca ccggcaacac gctgggggat gattgctacc
     7501 ggattacttc aggggctggt ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac
     7561 agttggattg tttatggcgt gcctgcgacc gtcgcgtttt catccgtttt actcttctcc
     7621 gtgatctctt ttaaacagaa gcgcctttgg ggatggctgg cgctggtgtt tattgccacg
     7681 acagggatga gcggttggct gaagtggcaa actgatggca tgaacccctg gagagctgaa
     7741 aaggcgattt gggattttgg ctgctatctg ctgttgatgg caatgttgtt gctaccgtgg
     7801 atacaacaaa gccttcgtat ccgcaatggc agtagccgct atagctactt ttaccagtca
     7861 gtatggcata acgtacttat attactggtg atttttctct ccaatggcct gacgtggctg
     7921 gtgcttttac tgtggggtga gttgtttaaa cttgttggca tcacgttttt caatacgctc
     7981 ttttttgcaa ccgactggtt tatgtatctt acatttggtc tggttaccgc actggcggtg
     8041 atcctcgcgc gtacacagtc acgtttaatc gactctattc aaaagttgtt cacgctaatc
     8101 gccacggggt tgctgccgtt agtatcattg ctaaccctga tgtttatcat caccctgccg
     8161 tttacgggcc tgagcgcgat ttctcgccac atctccgccg ccgggttgct gttaacgctg
     8221 gcctttttgc aattgatctt aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc
     8281 tggacggggc cgttgcgttg cctgattaaa accgctttgc tggttgctcc gctgtatgtg
     8341 ttcgtcgcca cctgggcgtt atggctgcgg gtcgctcagt acggctggac tgtcgaccgc
     8401 ttgcagggcg cgctggcgga gctggtgtta ctggtgtggt cgctggggta ttttgtcagt
     8461 atcgtctggc gtaatgggca aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt
     8521 tcgttattgg tgttggtgat actggtgctt cttaattcgc cggtgctgga cagtatgcgt
     8581 attagcgtga acagtcatat ggcgcgttat cagagcggca aaaacacgcc agaccaggta
     8641 actatctaca tgctcgaaca gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa
     8701 agcgatgccg ggtttatgaa agacccgaaa cgcgcgcggg atctgctgat ggcgttagat
     8761 ggagagcaac atcttcagga acaggtatcg gaaaaagtat tagccgaaaa tgtgttaatt
     8821 gcccctggtt ctgttaaacc ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat
     8881 aacgtgatga cctgtattga aaaagacgcc tgcgtactgg tcgagcaaga tctgaatagt
     8941 gatggtcagg cggagcggat cctgtttgct tttaatgatg acagagtcat tgtctatggc
     9001 tttgactcag acagaaaaga atgggacgcg cttgatatga gtttacttcc gaacgaaata
     9061 acgaaagaaa aattactcac agctgcgaag gatggaaaac tggggacgag gccgaaagcg
     9121 tggcgcgatc tgacggtaga tggtgaaacg ctggaaataa acttaagtaa gtgacaacgc
     9181 ttttatcgga tacgacgctg tatctgacca aaataatttg cgatttattt cataggcgct
     9241 ggtaagcgca gcacatcagg ccattaagtc gtaaaatccg gcagagccct ctctgccgga
     9301 catactcatt aatgaacttg cggatctgcc ggagacgcgt tgttgcggat ttcagcaata
     9361 tccatcgcat tgaacagata gtggttaccg cagtaatcac aatgcatgtc aatttcgcca
     9421 tcttccgcca ggatgctatc aacttcttca tcaggcagcg ttttcagcgc atcggcgcaa
     9481 cgttcacgcg agcaggtgca tttgaactcc acatcctgcg gatcgtaaac cgtcacctct
     9541 tcttcgtgat acaaacgcca caacacttcg tttgccggta aggtcagcag ttcttcggtt
     9601 ttgatggttt cggttagcgt cgccaggtgg tcaaagtcgt cctgctgggt attttgcgca
     9661 ggcattacct gcaacaacat accgcctgca gccggtttgc tgtctacgtc gccggtgcga
     9721 ataaacaggc gcgtcggcag ctgttcagaa cgcataaagt aatcttccag gcaggccgcc
     9781 agggtatcac cttccagacc aactacgccc tgatagcgtt cgccttcgct cggggtaatg
     9841 gtgatcacca cgtaaccatt gccgaccagc gttttcaggt cggcattttc tggaatttcg
     9901 ccctgcacgc gcgccacacc gcgcatttgc tggttattgt taccgttaat aaccgccaga
     9961 ttcatcggac cgtcgccctg cagctgtacg gtgatatcac catcaaactt cagcgtagcg
    10021 gttaacaggc tggtcgcaac cagcagttct gccagcacgt ttttaacggg ctgcggataa
    10081 tcgtggttct caaggatctg ttgcagggtt tccgaaacgg ttaccagttc gccgcgaacg
    10141 gcaaaatttt caaacagata gcgatgtaat tggtcatgtt gcggcataat catctctctt
    10201 gcaggtgaca gttattcact gtcgccgtgt ttaaatcgta acaggtcgcg gcgctctttt
    10261 ttgtccgggc gtcggtccgg gtgcggcatg gttaaggcat taagtttacg tgccatcgcc
    10321 attttttcgc gtttctctac actttccgca gtctcttcat acagcaaggc tgcctcgctg
    10381 gcggggcgac gctgttcagt aatcgccttt acaatcaccg tgcgttcgtc atttccctgg
    10441 cgcagagtga gcgtggcatt cagctcgacg attttgctcg gcttgctgcg ctgcccgttg
    10501 taatgcacct taccgccttc aatcatttca cgggccagcg cgcgggtttt ataaaaacgg
    10561 gcagcccata gccatttatc cagtcgaacc tcaacagcag gtttctcttt catggcgtct
    10621 ccttcacatt agcgagggga tcaggcggcg gtagtcattc agtgacggat ggcgttgata
    10681 ctgtttctcg gcaatcccgg aatcaggatt agtcacgccg aggcagtaac gaataccaaa
    10741 ttgcgcggca gcatcgagaa tcgcttcgct gtcatcaata aacagcgttc tttcagcttt
    10801 cagacccgta gcttcggcca ccgcatgcca taaccgctga tcctctttcg gataaccaaa
    10861 tgtgtgggtg gaaagtaata aatcaaggtg tgcgtccaga cccgtatgct caagttttac
    10921 cgccaggttg tgcggatgag cattggtgag caaaattcgc tgcttaccgc tggctttcag
    10981 tgcctcaaga aacggaatgg tatcttcacg cagtacggcg cgcggtccca tctcggtggt
    11041 catcgcacag atatccagac ccagttgctc actccagtaa tcaagacagt accagtttag
    11101 cgtatgctgt acgtcgtgat attgctggcg catatattcc atcgcttcct gtggcgtaac
    11161 cccgtttttc gcgccccatg tttcaggcac cagcttttgc cagaaatagt tatcgaaggc
    11221 gaggtcgagc aacgtgccgt ccatatccag cagaacggta tctacgtcct gccaggcaat
    11281 gttgatatgc atgagggaaa tctccagagt gaagcaattt gcgcgacagg gtagcataac
    11341 ctgccgcgca aacgtgttat tcgataaggc tttctgaagg ggtgatcagt tgcgggttca
    11401 ggcagctttc ataataggcc tgaatctcca tcatgcgagt gcgatgacgc tggtaacgac
    11461 gccaggcctg tacgccattg taaatggcgc tacccagcat cgtcagtagc agcaatgtgg
    11521 tgctgagata gcgccacagg ccggaacgat ccgggatcgg atgaaggcca atatgctgcg
    11581 taccattggc gtcagtgaag atttttgtga cgataccttc ggcgttaaac ggcgtatgca
    11641 tcagcatttg tgccagtttc tggaaagcgt tccactgttc ttgcggcggg tagtcgtaaa
    11701 gtgatgccga aggccagggc tgatcaacaa aatcgctgcc ttcgtcgctg acaatcagga
    11761 atccgccggg ggccggactg tttagtgatt gtgccgctcg cgccgtttca tgcgtgataa
    11821 acggcgcggt ggaggttgcc accaggttat ccagcgattc cgcactcacc gggcgtaata
    11881 acacattcac gccatcgagc ttcccggcgt tggcgcgttt taccagcgcg tcccagtctt
    11941 tactgttgcc gagattgacc agcgcatttt tcagtcgcac acagtcatct ttggcagaac
    12001 ataaatccgc tgtcttcagt acaatgtcgc caaaatcatc aagcaatacc atgccggatt
    12061 tttgaatcgc tgaacgtaat gaggcactga cgcgagattc atcttccggt ttagggtgca
    12121 gctggcgatt aactgcttca gtcaatgccg tcgctttgtt gaccagttca gattctggta
    12181 atggtaatga gcgggcgtca ttccagatga tctgcgagca gtcaaacggt aaaaaaggtg
    12241 aattggtttt cgcgctccag gttccggaag tgcgaatatt acacattccc gtaccgctaa
    12301 tacgcaatgt atcgcctacc cgcacgccag cgtcagccag ttgttttacg ccggtggctt
    12361 caatggtctg cgcgcctttc atccatgaga gagtgaattt cagcggcata tccagcggga
    12421 tccagaataa cagcataaac agcaccagca gcgagcccgc agcgataatc gtactgcgca
    12481 gccagtgctg taacggaaag ttttttactt catcatgcag cgaaagatat cgcccctgac
    12541 gcactacgtg gcggtcgaga tagatatcaa tatcggtttg ttgaccgaga tcctgagcaa
    12601 tgtatggctg ccagtgtgcg ggatagacca ggtcgataat accgagtgaa atattgttga
    12661 tctgttcctg atcgttttcg ccaaacaatc cccaacgacg gggtgtaccg cgcagacaat
    12721 gaatttctcg cagggaggat tttgccgggg gcgcgaatag cccccacaga cctgcgccca
    12781 gcagcagtaa tgcgccgcct gccagccacg gaacaaatac atccggggta atcaggcaga
    12841 aaaagaacat caggaaggag gcgacgatca gcaacgcttc acgcagcccg cgcggacgac
    12901 tcagcgcata ttcttcatgc gtttctttgc gaatattgag tagctcgatt tgctcactct
    12961 cttccccgcg aatcgacgcc tgcgtggacg gaacaggttg caaggcatag ccgcgcgttt
    13021 cctgcatgta ctcctgcagc gtatgaccgt tgagtgaaat aaccaacggc agcgaatcgg
    13081 tatgaatcag ttcaacggta ttctcatcgt tgatgtactg ttcccagaag gggggcaggt
    13141 ggacttctac cgaatcgagg tagtaacgcc atttattcgg atcgtcggta gagatgccgt
    13201 aacgtgtgat agcgtgtgtc agcatcatga cgttgttgct ttcggcattc agcgccagag
    13261 agagcggtgc cgcgctagct cccgttgggc taggcacctg taatacctgc gtcaggctct
    13321 caagataatt ttcaacggcg ctacgttctt caggtgtgag tttacgcgtt tgcgcatccg
    13381 cgaaggcgtt ggtccagggc agctgacgcc gtctggatcg cacttttatc agccatcccg
    13441 caagtagtga gcaggccagc aaagcagcta aaaaaatcac aatggtgctc atgctttccc
    13501 catcttactt agtctgtcag gcgtggtcag tcgcaccgga cacaatacat gattctgcgg
    13561 ttggctatcg gcaagcgtgg agtttaactt gagcatcatt aagcacatca cagtaaccgc
    13621 tgatcatagc aaaccctctg aaggcggaat atcagcaaat tcctggagtt atttcaacct
    13681 attgactttt aatttgattt gaggacgatt atttattaag ttacataaat gtaaataaca
    13741 tgcaattcac attgccgaaa gcgtgtccga tatcgcacaa taaccgctga tttcacagca
    13801 tagatcccac gatgagcaaa tcattacaaa aacccaccat tctgaatgtt gaaactgtag
    13861 cccgttcccg actgtttacc gtcgagagcg tggatctgga gttcagcaat ggcgtgcggc
    13921 gtgtttatga acgaatgcgt ccaaccaacc gggaagcagt gatgattgtg ccgattgtgg
    13981 acgatcacct gatcctgatc cgcgaatacg cagtgggaac tgaatcctac gaattaggtt
    14041 tttcgaaagg attaattgat ccgggtgaaa gcgtctacga agccgctaac cgcgagctaa
    14101 aagaagaggt tggatttggt gcgaacgatc tgactttttt gaagaagctc agcatggcac
    14161 cgtcttactt ttccagcaaa atgaatatcg tggtcgcgca agatctctac ccggaatcgc
    14221 tggaaggcga tgagccagag ccgctaccac aggtgcgctg gccgctggcg catatgatgg
    14281 atttgctgga agaccctgac ttcaacgaag cgcgtaatgt cagcgcgctg ttcctcgtgc
    14341 gcgaatggtt gaaagggcag gggcgagtgt aaatgcgtga agtttgcctg atgcacaacg
    14401 cttatcaggc ttatggggtt cctgcaatat attgattttt cacgattttg ttagccggat
    14461 aacgcgttca cccgcatccg gcataaacaa atgcactttg tcagccaact gaaaaggcgc
    14521 cgaagcgcct ttttaatcag aacaattcct gtgcctcacc attatcgata atggtcgttc
    14581 ccacctcgtg caccgcctgt tgtgtcggct gcgtaccttc gatgaaatac tcttcgcggc
    14641 tgttgccacc attagctaac tgcccggtgc tgcgatcgat attcaccgtc acaatacccg
    14701 gaggcggcgt cagcggctgc tccggcacac cttcaagaac ggctttcata taagcgtccc
    14761 atgcaggctg ggcactcttg gcaccgcctt cgtaacctga gatctgatct ttaatcgctc
    14821 cggaagccgt tgtatgaccg agattacgac ggtgatcatc aaagccaatc cagaccgagg
    14881 tcacaacgcc cggaccgtaa cccgagaacc acgcatcttt cgaactgtta gtggtaccgg
    14941 ttttcccgcc gatatcgcga cgctgcaaat cacgacctgc acgccagccg gtaccctgcc
    15001 agcctggttc accaaagata ttggtgttca aagcactctt aatcaggaat gccagcggag
    15061 tgttgatgac gtgcggtgcg tactcctgcg cgccagtctt cgccactaac gcctgatttg
    15121 cctgccccag ctgcggcatt ggtacagaaa cattctgctg ctcgcgggag atagcgacat
    15181 cttcaacatc gttattttcc agcacgttcg atttctgcgt atcaccgtaa atcactggaa
    15241 tatcgcattc cgggcaggct actttcggtt tcgcttcgaa aatcacgccg ccctgatcgt
    15301 tttcaatttt gctgataaac cacgggtcca ccaggaagcc gccgttcgcc atgaccgcgt
    15361 agccgcgcgc cacctgcatt ggggtgaagg acgctgaacc cagcgccagc gattcggtgt
    15421 ggacaatgtt ttgcgccggg aagccgaagc gttgcagata ttctgcagcg tagtcgacgc
    15481 ccatcgcccg cattgcacgt accatcacca cgtttttcga ctgacccagc ccctgacgta
    15541 agcgaattgg accagcatac tgcggtggtg agttcttcgg ctgccagtca gaaccggcac
    15601 ctgcatccca gcgagaaatt ggcacatcgt tcaacatact tgccagcgtc agacctttat
    15661 ccatcgccgc ggtgtagagg aacggtttga tgttggaacc cacctgacgc agtgcctggg
    15721 tggcgcggtt aaacttgctc tgattgaaat caaagccacc gaccagcgcc ataacggcac
    15781 cattttgcgg attgatcgac accagcgccg agttcacttc cggcacttgt gccagccacc
    15841 atgcatcgcc aacctgacga acccagattt gctgacccgt ttgcagaaca tcggtcactt
    15901 tacgcggcgt cggtccctgc tgagtatccg aacggtaagg acgcgcccag cgaacgcctt
    15961 ccatactcaa tgcgacggtc gacccgtccg ccagcatcgc cgtcgctccc tgaggattgg
    16021 cgctggtgac tgcggcaggc agcagcggac cataggtcgg cagcgccttc agcgtatcgg
    16081 taatcttgtt gttatcccac gccgactcgc ccactttcca cagcacattt gccgggccgc
    16141 gatagccgtg gcgcatgtcg tagtccagca cgttattacg taccgcctgc tgcgcggcct
    16201 gctgcacttt gtgggtgatg gtggtgtaaa tgcgataacc gtcttcatag gcactttcgc
    16261 cataacggtt atacatctcc tggcgcacca tttcgctcag gtacggcgca gagaaagcaa
    16321 tctccggcgc gtgataatta gcgttaatcg cctcagtgcg tgtctgatcg aactgttgct
    16381 gggtgatata cccttcatcc agcatccgcg acagtacgac gttacgccgc gcgacggcac
    16441 gatccattga gtagagcgga ttaaaggtgg aaggcgcttt cggcaacccg gctatcaccg
    16501 ccatttcgtt cagcgtcagt tggtcgaccg ttttaccgaa atagacttgt gccgcagcac
    16561 cgacaccata ggcgcggtaa ccaaggtaaa tcttgttcag ataaagctcg aggatttcgt
    16621 ctttcgtcag caactgttca atgcgaatcg cgaggaagac ttccttaatc ttacgcatca
    16681 gcgtgcgttc tggactgagg aagaaatttc tcgccagctg ctgggtaatg gtactcgccc
    16741 cttgtgacgc gtggccggag aacagcgcca cacttgccgc acggaagatc cccaccgggt
    16801 caacgccgtg atgctcgtag aagcggctgt cttccgtcgc gataaaggct ttcaccatct
    16861 ccggtgggat ttgatccaac gtaaccggaa tacgacgttt ctcaccgtat tgagcaatca
    16921 gctcgccatc ggcgctgtaa atctgcatcg gaatttgcag gcgaacatct tttaatgtcg
    16981 ccacatccgg cagttgtggc tcgatgtagc ggtataggcc ataaatcgag cctgctccca
    17041 gcagaatgca acagactgca aggatcaaaa aatactttac gaacttcact ggaaatttcc
    17101 catttagttt catttgggca gtttataaac aaacgcgcgg tagtataaag gcaagccagg
    17161 cgcattgata tacccgtcag agttacgggt gataaggaga tcatcacaat ggcatttaag
    17221 atctggcaaa ttggtttgca tttacaacag caagaagcgg cagcggttgc gatcgtgcgg
    17281 ggcgcaaaag aatgcttttt gcaacgctgg tggcggttgc cgctggcgca cgacattatc
    17341 aaagatgggc ggattgttga tgcgcagcag ctggctaaaa cgttgttacc gtggagtcgc
    17401 gaactgccgc agcgtcatca cattatgttg gcgtttcccg ccagtcgcac attacagcgg
    17461 tcatttccgc gcccgtcgat gtcccttggt gagcgggagc aaacggcttg gctgtcaggg
    17521 acgatggccc gcgagctgga tatggatccg gactccctgc gcttcgatta cagcgaagac
    17581 tcactcagcc ccgcttataa cgtgactgcc gcgcaaagca aagagctggc aacgctgctt
    17641 acgctggcag aaaggttgcg tgttcatgtt agtgcgatca ccccggatgc cagtgcatta
    17701 cagcgattcc tgcctttttt accttctcat cagcaatgtc tggcctggcg tgataacgaa
    17761 cagtggctgt gggcgacgcg ctatagctgg gggcgcaaac tggcggtggg gatgactagc
    17821 gcgaaggagc tggcggcagc gttatccgtt gatcccgaca gcgtcgcgat atgtggcgaa
    17881 ggcggatttg acccctggga ggccgtttct gttcgtcagc cgccgctacc gccgcctggt
    17941 ggagactttg ccatcgcgct ggggctggcg cttgggaagg cgtactgatg aacccgccaa
    18001 ttaatttttt gccctggcga cagcaacgcc ggaccgcttt tctgcgtttc tggttgctga
    18061 tgttcgttgc gcctctgctg ctggccgtcg ggataacgct aatactgcgt ctgacaggca
    18121 gcgccgaagc gcgcatagac gcagttttgc ttcaggcgga acaacaactc gcccgcagct
    18181 tacagataac gaagccacgt ttgctggagc aacagcagtt acgcgaacag cgttcgcaac
    18241 ggcagcgcca gcgacaattt acccgcgact ggcaatctgc gctggaagca ctggcggcgc
    18301 ttttacccga gcacgcctgg ctgacaacga taagctggca gcagggaacg ctggagatca
    18361 aggggctgac aacaagcatt accgcgttaa acgcactaga aacgtcactc cgccaggatg
    18421 cgtcttttca tctcaatcag cggggagcca cgcagcagga tgcgcaggga cgctggcaat
    18481 ttgagtatca gttaacaagg aaggttagcg atgaacatgt tctttgactg gtggttcgcc
    18541 acatcacccc gcctccgcca gttttgctgg gcattctggt tgctgatgtt agttacgctc
    18601 atttttctat catcgacaca ccatgaagag cgcgacgcat taattcgact acgggcaagt
    18661 catcaccagc agtgggccgc actgtatcgc ctggtagaca ccactccctt cagcgaggaa
    18721 aaaacgctgc ccttttcgcc actggatttt cagttatccg gcgcgcaact ggtttcctgg
    18781 catccatccg cgcagggagg cgagttggcg ttgaaaacgc tgtgggaagc agtgccgtcg
    18841 gcatttacac ggctggcaga gcgcaacgtc agcgtgagcc gtttttcgtt aagcgtggaa
    18901 ggtgatgatc ttttgttcac gctacaactg gagacgccgc atgagggtta aacgctggtt
    18961 gttggcaggt attgcattgt gccttttaac cggtatgcgt gaccctttta aaccgccgga
    19021 agatctatgc cggattagcg aacttagcca gtggcgctat caggggatgg tagggcgagg
    19081 cgagcgcatc atcggtgtaa taaaagacgg gcaaaagaaa tggcgacggg tgcagcaaaa
    19141 ctatgtgctg gaaaacggct ggacaatttt acagctgacg ccagacgtac taacgctggg
    19201 taccgggaca aactgcgaac cgccacaatg gttgtggcaa cggcaaggag atacaaatga
    19261 agcaatggat agccgcacta ctgttgatgc tgatacccgg cgtacaggcg gcaaagccgc
    19321 aaaaagtgac gctgatggtg gatgacgttc cggtagctca ggtgttgcag gcgctggctg
    19381 aacaggagaa gttgaacctg gtcgtgtcgc cagacgtcag cggtacggtg tcgttacatc
    19441 taacagatgt tccctggaag caggcactac aaactgtagt gaaaagcgcc ggactgataa
    19501 cgcggcagga aggcaacatt ctctcagtgc attccattgc ctggcagaat gacaatatcg
    19561 cccgccaaga ggcggagcag gcgcgggcgc aggcaaatct gccgctggaa aatcgcagta
    19621 taaccctgca atacgccgac gcgggagaac tggcgaaagc gggggagaag ctactgagtg
    19681 ccaaagggag tatgaccgtc gataaacgca ccaatcgcct tttgctacga gataacaaaa
    19741 cggcgttaag cgcgcttgaa cagtgggtag cgcaaatgga tctgccggtc gggcaggttg
    19801 agctgtcggc gcatattgtc accattaatg aaaaaagttt gcgtgattta ggcgtgaaat
    19861 ggacgctggc cgatgcgcaa cacgctggtg gcgttgggca agtcaccacg cttggtagcg
    19921 acctctccgt agcgacggcg acaacgcatg tcggttttaa cattgggcgc atcaacggac
    19981 gcttgctgga tcttgagctt tccgcgctcg aacaaaaaca gcagctggat attatcgcca
    20041 gtccgcgtct gctggcctca catcttcagc ctgccagcat taaacagggg agcgaaattc
    20101 catatcaggt ttccagcggg gaaagtggcg cgacgtcggt ggaatttaaa gaggccgtcc
    20161 tggggatgga ggtcacgccc acggtgttac aaaaaggtcg catccggctg aaattacaca
    20221 tcagccagaa cgttccgggg caggtgctac agcaggccga tggcgaagtg ctggcgattg
    20281 ataagcagga gatcgaaacg caggtcgagg taaaaagcgg agaaacgttg gcgctgggcg
    20341 gcatttttac ccgtaaaaat aaatcgggtc agga
//
LOCUS       DADLSE010000070        19970 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLSE010000070 DADLSE010000000
VERSION     DADLSE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.070"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..758
                     /locus_tag="JN692_002442"
     CDS             <1..758
                     /locus_tag="JN692_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAY7264729.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFD
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
     gene            complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN692_002443"
     CDS             complement(827..1783)
                     /gene="yjiA"
                     /locus_tag="JN692_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7264730.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQMGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(1794..1997)
                     /locus_tag="JN692_002444"
     CDS             complement(1794..1997)
                     /locus_tag="JN692_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7264731.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN692_002445"
     CDS             complement(2047..4197)
                     /gene="btsT"
                     /locus_tag="JN692_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7264732.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            complement(4590..5102)
                     /locus_tag="JN692_002446"
     CDS             complement(4590..5102)
                     /locus_tag="JN692_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7264733.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN692_002447"
     CDS             complement(5120..6682)
                     /gene="hpaB"
                     /locus_tag="JN692_002447"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HAY7264734.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            complement(6909..7765)
                     /locus_tag="JN692_002448"
                     /pseudo
     CDS             complement(6909..7765)
                     /locus_tag="JN692_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN692_002449"
     CDS             complement(7873..8763)
                     /gene="hpaA"
                     /locus_tag="JN692_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7264735.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN692_002450"
     CDS             complement(8773..10128)
                     /gene="hpaX"
                     /locus_tag="JN692_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7264736.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMAIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            complement(10150..10893)
                     /locus_tag="JN692_002451"
     CDS             complement(10150..10893)
                     /locus_tag="JN692_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7264737.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGLADLSADMGYAGNPQHPEVQAANEQLAKRYLELGALFVAVGVDTTL
                     LARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN692_002452"
     CDS             complement(10904..11707)
                     /gene="hpaH"
                     /locus_tag="JN692_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7264738.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            complement(11775..12155)
                     /locus_tag="JN692_002453"
     CDS             complement(11775..12155)
                     /locus_tag="JN692_002453"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7264739.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMEGRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN692_002454"
     CDS             complement(12165..13016)
                     /gene="hpaD"
                     /locus_tag="JN692_002454"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7264740.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN692_002455"
     CDS             complement(13018..14484)
                     /gene="hpaE"
                     /locus_tag="JN692_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7264741.1"
                     /translation="MKKVNHWINGKNVADNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTTTGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            complement(14481..15770)
                     /locus_tag="JN692_002456"
     CDS             complement(14481..15770)
                     /locus_tag="JN692_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7264742.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            16042..16488
                     /gene="hpaR"
                     /locus_tag="JN692_002457"
     CDS             16042..16488
                     /gene="hpaR"
                     /locus_tag="JN692_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7264743.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAEST
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            16607..18271
                     /gene="tsr"
                     /locus_tag="JN692_002458"
     CDS             16607..18271
                     /gene="tsr"
                     /locus_tag="JN692_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7264744.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPCQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(18320..19681)
                     /locus_tag="JN692_002459"
     CDS             complement(18320..19681)
                     /locus_tag="JN692_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264745.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSIRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREKLGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
BASE COUNT         4846 a         5087 c         5247 g         4790 t
ORIGIN      
        1 attatactaa gattttagag aatgattttg aacacccttc aatacgacga aaaatagcat
       61 cgctaagtaa tttaggggta gatgagtgga ttcctccggt tatggcattc atgaatcgaa
      121 tggctcgtac agaagatttt aatttagatg atttttctca atttattacc gcctttgaga
      181 aggtatatat gcatgggtgg ttaaaaaaac aaattaaaag ccaaagagaa atggtttgtt
      241 attcagctct ggtagcaata aataatgata tgccttttga ttctgtgata aatcagataa
      301 accaacatgc agataattca ggttttatag ctgctctgga tgaagattta tatgagcctc
      361 gtccgaatca agtgaactta ataaaagcaa ttttgcttcg cttagatatg gagcaacaag
      421 atgagagcgt gattaaaacc tatacgggga gaatcacaat tgagcatatt cttcctcaag
      481 cattagttaa tgaatactgg attaatagat tccaacctca agagcatgtc tactggctgc
      541 ataaaatagg taatttaaca ctcataagtg gttccaaaaa ttctgaagca caacactatg
      601 attttatcaa aaagaaatct atttatgaga aacttaatag taaaagctct tttgatttaa
      661 caaaagatgt ttgtaattct tcagaatggg gattggctga actgaaaatg agacatgaaa
      721 aaatgaaaac gcaattgaag aagctttggt tggtctgaca tatctaaaga gttccactcg
      781 taataatgtt gcggtggaac tctcaaaaag ttgatctttc ccgaacttac ttcctcaatc
      841 ccgcaaacgc cgcccgaatc tcttcctccg gcaactgaat cccgataaac accattgtgc
      901 tatgcggttt ttcatcgccc cacggcctgt cccagtcggc gctgtagagg cgctggacgc
      961 cctggaacag caggcggtta ggttcgccgt caatccacag catccctttg taacgcagca
     1021 gtttatccgc cgattccagc agcaggtttt ccatcacgcg ggaaacttcg ctgatatcta
     1081 ccgggtaatc cagttccacc acaattgacg aaatatcgtt ttgtttatcc gcgataaagt
     1141 ggaaacgcgg tttggtgctg acgacgttct cttccagcat aaaaccgttg gtgttgaaca
     1201 gcaggcccag gtcgatgtcg ccgtgggtga cggtgtagac cggtgcgcgg gcgttgatgc
     1261 gcgccaggcg ttcacgcagt ttctctgctt cgcctgcgac gtcggtttta gtcagcagaa
     1321 tacggtcggc gtagcccatc tgggactggg cgatggtgaa ctggttcatc tgctcatcgg
     1381 catgtaccgc atccaccagc gcaatcacgc cgtccagcag gtagcgctgg cataaaatct
     1441 catgggagaa aaaggtctga ataatcgggc cgggatcggc catgccggtg cattcaatca
     1501 ccagacgatc gaactgaata ttgcccttgt cgagattatc cagcaggtcg agtagtgcgt
     1561 cctccagctc gttggagcgc gaacagcaga tgcagccgtt ggtcagcgtt ttgatctgcg
     1621 tggcgcggtc gccaatcagt tgatcatcaa cggagacttc gccgaattcg ttttcaatca
     1681 cggcaatctt gtagccgtgt tgttcgttaa gaatatggcg cagcagggta gttttgcccg
     1741 cgcctaaaaa accggtgagt agggtaactg caatcgggtt catgctctct ccattaacaa
     1801 cagcgcatac cgcctttacc atcgccgccg tagcgcgcgt tctggcgttc gcggaagaat
     1861 tcttcatagc tcatgtacgg cttgtcggga tggttggtct tcatatgctc gacgtagttg
     1921 tcgtagtctg gaataccaat caacatcttc gccgcctggc cgagatattt ttttgcctgt
     1981 cctaagttac caaacatagt tcactctgat aagaacaaag ccccgccgaa gcggggctaa
     2041 acacggttag tggtgcgaag agatcttcac gccgccttcc gggattggaa cgtacggtgt
     2101 ttctttgtca gtacgtttgt cgctgttacg caccgcaagc caggttttga aaccgtagaa
     2161 gatgatgctg tacaccacaa tcaggaacag aatactcagg cctgcgttgg tgtagttgtt
     2221 cacaacgatg tggttcatgt tggcaatctg ctgcgccgtc aggtcagtac cgttagcaat
     2281 cttctctttg tactggcttg ccatgtagaa gaagccttcc atctgcgggt tggtgctgaa
     2341 cagtttcaga cccagcgccc aggtggtgca gataagcagc catacagccg gaacaacagt
     2401 tacccagatg tattgggtgc gcttcatctt aatcagcaca acggtgccca gtaccagcgc
     2461 tacggctgcc agcatctggt tggagatacc gaacagcggc cacaggctct taacgccgcc
     2521 cagcggatcg accacgccct gatacagcag gtagccccac agacccacac agcccgcagt
     2581 accgatgata ccggcaacca gagagtcggt ttttttcagg aacgggatga agttacccag
     2641 caggtcttgc agcataaagc ggccagaacg ggtacccgca tccagcgcgg tcaggatgaa
     2701 cagggcttcg aacagaatac cgaagtgata ccagaagccc atgtcagcca tcggcagcac
     2761 tttgtggaac acgtgagcga tacctaccgc cagcgtcggc gcaccacctg cacggttcag
     2821 gacagaaggc tcaccaatgt ctttcgcggt ttgcaggatc tgctctggcg aaatcacgaa
     2881 gccccaggag ctgacggtcg ctgccgcgtg tgcggtaacg tctttcagct gcgccatgat
     2941 gatcggcgcg ttctcgccac ccatttcatg caggttaggc atggtgatgc caaggccagc
     3001 aggcggggtg ttcatcgcga agtaaagacc cggttcgatg atggacgctg caaccagcgc
     3061 cataatcgcc acgaaggact ccatcagcat tgcgccgtag ccaatgaaac gcgcgtcggt
     3121 ttcgttagcc agcagtttcg gcgtcgtacc ggaagagatc agcgcgtgga agccagatac
     3181 cgcaccacag gcgatggtga tgaacaggaa cgggaacaga gcgcctttcc acagcgggcc
     3241 agtaccgtca atgtactggg tcatcgcagg cattttcagt tccgggttca gcaccacgat
     3301 acccagcgcc aggccgacga taacgccgat tttcaggaag gttgccagat agtcgcgcgg
     3361 tgcgaggatc agccacaccg gcagcagtgc ggaaacaaac gcatagccaa tcagcgcgaa
     3421 ggtaatggtg gtgtctttaa aggtcagtgc cggaccccag tacggatcgt gagcaatcac
     3481 gccaccgaag tagatagagg caaccagcag cacgatacca ataacagaga cttcacccac
     3541 acgccccgga cggataaagc gcatgtagat acccataaac agcgcaatcg gtacggttga
     3601 gcaaacggtg aagacacccc acggactttc ggccagggct ttaaccacaa tcagcgccag
     3661 aacggcgagg atgatgatca tgattaagaa acagccaaac agcgcgatag tccccggtac
     3721 tggccccatc tcttctttga tcatctcacc gagagatgcg ccgttacggc gagaggagat
     3781 aaacagcacc ataaagtcct gtaccgcacc ggccagcact acccccgcca gcagccacag
     3841 cgtgccaggc aggtagccca tctgcgcggc gagaaccggt cccaccagcg gaccagcacc
     3901 ggcgatagcg gcgaagtggt gaccaaacaa cacgtaacgg ttggtcggaa cgtagttcag
     3961 accgtcgttg ttaataaccg caggcgtcgc gcgcgtgggg tcgagtttca tcaccttctg
     4021 ggcgatgtac agactgtagt agcgatacgc caccagatac accgacacag aggcgaccac
     4081 gatccacagg gcgctgacgt gctccccccg acgtaatgca actaccgcga ggcagaatgc
     4141 accgatgatt ccgagaatca cccagggtat gtgcttgaat agttttttcg tatccatagt
     4201 aaaacctggc attttactcg tcgtcttttg gcttacaggt gcggcagctg cgttcgttca
     4261 cctctgacac ttacttttgt aagctcccgg ggattcactc acttgctgcc ttcctgcaaa
     4321 ccaaaatcca tagagtaatt tgttaatcat aggccgaagc cgttctaggt ttgcgttgcg
     4381 tttgaggagg taaattgacc gctatcctgc agtgatgttg ccagagatac gtgcgcgtaa
     4441 agtaaggtaa ataactgagt ggttatttta gggatgtaag cggtcaggaa tggcggtgag
     4501 cggttgggca agaattattc atcgggtaga tatgtgattg agatcacaat aaaaatgccc
     4561 cttcgacgaa tgcgtgaagg ggctggagct taaatcgcag cttccatttc cagcatcacc
     4621 ggatggaaac ggcgtttaaa gtaaataagt ccgtgacctt ctgcactgag gatgatgttt
     4681 ttaatctcca ccagatacac cagatgtgtg ccaatcgcct gcacatcgcg gatctctcct
     4741 tcaagactgg ccagcgaacc ttttagcacc ggctgcgcca gcggaccttt ttgccagcat
     4801 gagaggctaa aacgctcttc catcgccatg cctgtcatgc ccgcgaagtg gcgcgccatc
     4861 agttcctgct catggttgag gacgttgacg cacaacttgc cgttgccctg aaaaaccggg
     4921 ttcatcgcac tgttggcgtt aatgcacacc atcagcgacg gtggtgtatc cgtgaccgag
     4981 cagacagccg ttgccgtaat cccgcattgt ccggcgtcgc cctcggtggt gataatattt
     5041 accgctgccg acaggctggc catcgcgtca tgaaagcgca ggcgttgttc atctaattgc
     5101 atcttaacct cctgctgcgt tatttcagca gcttatccag catgttgata tcgtcgttgt
     5161 tgtgcaggtg cggcacagtc cagccgttct ggtcgtattc cgacaggcag cgatcaacca
     5221 tcgccatcat cttgtccata ttgccggagc tttgtgcctg gcgcagacat tgcaggcgaa
     5281 tctcatcctg gctaccggag tagttgattt catacagctc gtgacgacca ccaaattcgc
     5341 tgccaatggc gtcccacatc agtttgagga tcttgatgcg ctggacgtga tccataccgt
     5401 tcgaaccgcg cacatacttc gccagatact ggtcgatctg cggattgttc aggtcacggg
     5461 cactggaagg gaggtagatc aggccactgg taacgttgcg ttctataatg tttttgatct
     5521 tcgcgtaggc cattggtgcc agtacgcgat aggtttgcag tgcggcatga tccggtaaat
     5581 aagccccgtt gacccacggc gtcgcttctg aacacatcga gtcactcaat gcccagaagg
     5641 tgttgcgcca cgccaccact tcaccgagat cggcctgcac accacggaac tccagggtgc
     5701 cggtacattc gagtgatttt ttcagcagtg ccgtaatgaa gtcgagtttc actgccaggc
     5761 gcacacaggc ttgcagcgga tacatacggg cgaaaccgcc ttccatcgtc cagcgacggc
     5821 agcgatcaaa atcgcggtag atcagcacgt tttcccatgg gatcagtacg ttatccatca
     5881 ccagaatcgc atcgttctca tcgaagcggc tggagagcgg gtagtcgtat ggcgagccgg
     5941 tagcacccgc gaccatctca taagaggcgc gggagattaa tttcacgcca tcggcatcca
     6001 ttggcgcaac gaacatcagt gcgaagtccg ggttttcgcc catcacttgt gccgagccga
     6061 agccaatcat gttgtagtga gtcagcgccg agttggtggc aaccactttc gcaccgctga
     6121 cgataatccc ggcgtcagtc tctttttcca gcttgatgta aacgtctttc actttatcgg
     6181 tcggcaaatg acgatcgatc ggtgggttaa caatcgcgtg gttaaagtag aggccagttt
     6241 cctgaatacg ggtataccag ttacgggcgt tctgctcgaa ctgaccgtaa aagcccggat
     6301 tcgcgcccag tgcgcaaccg aaagcggctt tgtagtctgg ggtacggccc atccagccat
     6361 agctcaggcg tgaccattca gcgatggcgt cgcgttgctg gcgcaggtcg tcggcacttt
     6421 tcgccacgcg gaagaattta tgggtatagc cgccgctgcc ggtgtcggtg ttccaacaca
     6481 gagagtcctg catctccggt ttgtgcagcg cgtcgtacag ttgggcaaca gacgctgccg
     6541 cattacgaaa tgccggatga gtggtgacgt ctttcactcg ctcgccatag atatagatct
     6601 cgcgaccatc ctgcaggctt ttcagatact cttccccggt gaaaggacgt tgggtactgg
     6661 cgcggaaatc ttctggtttc atgtcgacct ctacagtatt aatcggaata cgattgttaa
     6721 ttttgtgtgt tgtttttgtt atttaattga agcgttgaac gcggttgtcg ggaagagacg
     6781 tttcaggcga acgttggtac ttttcgacag caaggtggag ttaaatagta aactgcgatc
     6841 gtgatcaact tacagtaagg caaatcataa tattccccgc taacaagggc taacggggac
     6901 ggaatttttt atggggcgaa tggaatggta atcgtcatat tttcatgacc aacgacgata
     6961 gggcctgaat agttttctgt gcttcttgct tccatgtttc atccggaacg gtgattggta
     7021 ccaggtggct aaggatgagt tttttcactt ttgcttcatt ggcaattttg cctacatcct
     7081 caatagtcgt atggtgagaa gcaatggctt ttgccagtgt tttaccattg ccaatactgt
     7141 ctgccagctt agcgacggag ggctcatgta cggcctcatg cacaagtata tcagcgcctt
     7201 ctgagaaagt tgccagtggg ggaaagtacg aggtatctcc tgagaagaca attcgcttgc
     7261 cttgtgtgtc gaagcgataa gcaaaagcct caccgtcagg gaaaggagaa tgagggacct
     7321 ttaatgcagt cacggtcacg gtatcatctt tataaactac gccttcacca acatcttgag
     7381 ctgtaacgag tttgcgagga tcgggttttc cttcttcttc catacgcagt gtgatgtccc
     7441 gatcgaagac ctgccaactg gcagcggtca tggctcgggt gcctggcgga ccgaatacat
     7501 gaatagtttt gtcctgcaga ccgctagccc atgcattcat taagactgat ggatagtcca
     7561 gaatatgatc actgtgtaaa tgggtgagta aaatattatc aatctggcgc agtggaatat
     7621 tattttttac cagttgtaag ctggccccgt aaccagcatc aattaactag gttttattgc
     7681 caataataag tgccgttgcc atcggtaaac ggtcagcttt taataaagaa gggccacctt
     7741 tcgttccaag tagtacaatt ttcatttatt gcggttcgga ggcgtaggtg aatgccactg
     7801 gcgcaatcag gcacgccaca aatagtgagc gtaaagatat tttcatgtcg agtctctcag
     7861 gttgataaat ggtcagcgac cacggaattg tgttggtgag caacccatca ttcggttgaa
     7921 aaaacgggcg aaataggcgg gatctttgta gccaagatca agggctatct ggttgatgct
     7981 ttgctcgcta tagagcagaa gccgtctggc ttcccggctc actcgttcga aaatcaatct
     8041 cttcggcgat tgattggcaa aacgtcggca cagctcggta agccgtgatt cactcatacc
     8101 gagcattgtt gcatattcag ggaccattaa atgttggcga taattttcgt caatcagccg
     8161 attaaacttc tggaataaac acatctcgcc gcgcacgctg catgttgaaa tatcatttgg
     8221 tggtgcttca cgcagtagga ttgtaaaaat actttgcgcc agtgattgca atagaatttc
     8281 ccgtcctggc ttattttgtt gaaactccag ggatattatc ggccaataat gatttaatgc
     8341 cgccagtgta tgttccattc cctccagtga cagacagata ccttgtagca ttgcggcatc
     8401 tccactacca ggccagagct tttctaccag gggccaaatt aactcctgac gtacggtcag
     8461 aacatgccca tcgctatcag gatcagtaaa gaaagtatgt ggcaccgatg gtggtgttag
     8521 tacaaaaagc ggtgcgcgca cagaatagaa gtgctcatcc agttgtaaag taatgcgtcc
     8581 agtgaccaga taatggagtt gaaaatatct atcgtgccag tgagggcgca tatcgcgtcc
     8641 aaagaaattc gctaaccggg caaatgtttc ataatgcaca tcttcaccag cgtagcgggc
     8701 atcatattcc cggaccaaat caatattttc ataaccatgt tcaaagatag cttcactgga
     8761 catcatgact ccttattgcg gttttggtgc gataagttca gatgaaagag agtgttcccg
     8821 actccccata gggattcggg tcatgactaa cgcgcccagt accagcaatc ctgctacaaa
     8881 ccacaaacct gagctaaaac tgccggttgc atctttcaaa ataccgataa gtaatggact
     8941 gactgctgaa cctacattgc cgacagcatt gatgacagcc atcgcaacgg cacgcgattg
     9001 caagcttata acttgatccg gtgttgtcca aaagatagcc atcgcggtaa atgatcccat
     9061 tgaggccata atgatgccgc tcagttgaat gaggctgtga tctgtcgcag aagcgagtaa
     9121 ccatccggca gcggcaaaca ggtagggaag aatcgtatgc tttttgcgtt ctttaagacg
     9181 gtcggaacga cgactccacc agatcatgcc aaaaattgtg cagaattggg ggatggccgc
     9241 cagcaaacca atcatgatgt tgctactgcc agtattgaaa ctctgtagga tttgcggcgt
     9301 ccagatatta atcgcactca gagtattggt caggcaaaaa taagcaagtg tatatagcat
     9361 gatggctggg gtcaggactt cacgtaaggt tgagcgtgga acagatgcat gggcaaatgc
     9421 actctcttgt tcacggagaa tcatcgtttt taaagcctgt ttttcgtttt catcaagcca
     9481 ggtcgcctgg tctggtgtgt cgttgagata aaaccaggtg acgacaccaa gaatgaccga
     9541 tggcagacct tccagcaaaa agagccattg ccagcctttt agattccaca gtccatccaa
     9601 ggccagaatg tagcctgaaa ggatcaatcc cagcatcata gtgactggca tcgctatcat
     9661 aaataaggca ttggctctgg cccgatgata tgccgggaac caccaggtca gataaaccaa
     9721 aataccaggt aaaaaacccg cctcagcaat accaaccagc atacgcagta gatagagtgt
     9781 ttcggggctt gttgcaaaca ttgtgcaggt ggaagcaatt ccccagacca ccatgatccc
     9841 agcgatccat cgacgagcgc caattttcgc taacatcata ttactgggga tcccgcacat
     9901 gacgtacgtg acataaaaga gtgtcgcggc gaggccaaac attgtggacg tcaggccaag
     9961 atccttgccc attgtcagtc cggcaaatcc tatattgatg cgatcaagaa acgaaaaaac
    10021 aaacagaata aaaagaaaaa tgattaggcg acgaaataat ttattaatga cacgatgttc
    10081 aatttcaaca gttttattat ctttatattg cagggtagag gtcgtcatga tgcgctccac
    10141 atcgtacgtt tagtagacag agttgttgtt ttgcttcgcc atcgaaatag aagaactatt
    10201 accaaaccgt gctgccagcg cttcggcggc gcgggcgagc agggtggtgt caacgccgac
    10261 ggcgacaaac agcgcgccca gttccagata gcgttttgcc agttgctcat tggcggcctg
    10321 tacttccggg tgctgcggat taccggcata acccatatcg gcgctcagat ccgccaggcc
    10381 gataaacacg ccgtcgaccc cttccacgtc cagaatctgc ggtaagttct tcattgcctc
    10441 acgcgtttcg atctgcacca gcacgcacat ttgatcgttg gctttttgca ggtaatcagg
    10501 aatgcgattc cagcgcgagg cgcgagccag cgcactgccc acaccgcgaa taccggcggg
    10561 gggataacgg gtggcgcgta ccgctttacg ggcttcgtcg gcgttttgta ccatcggcac
    10621 cagtaaggtt tgtgtgccta cgtccagcag ttgtttgatt tgcaccggat cgttccacga
    10681 tgggcgtacc actggctggc tggggtaggg cgcaatcgcc tgtagttggg tgagcacggt
    10741 ttgcacgtta ttcggcgcgt gctcaccgtc gatcaataac cagtcgaatc ctgctccggc
    10801 cagtaactcc gcgctgtagc tgctgctcag ccccagccat aatccaatct gcggacggcc
    10861 tgctttcagc gccgctttaa aactgttttc catcgttctc tccttaaaca aagcggcagc
    10921 taatggagcc catgttgccg taatcgacgt ggaaggtgtc gcccttacgc gccggaaccg
    10981 ggcgggtgaa cgaaccaccg agaatgattt gcccggcttc cagttgtacg tcatacggtg
    11041 cgagtttgtt cgccagccag gccacgccgt ttgccggatg attcagcaca ccagcggcga
    11101 cgccggtttc ttcaatcacg ccattgcgat acatcagggc ggagatccaa cgcagatcca
    11161 gttcgtctgg tttgatcgga cgacctccga ggatcactcc ggcgttggcg gcgttatcgg
    11221 aaatggtgtc gaatactttg cgcgggcgct gggtttccgg atcgatgttg tggcagcgag
    11281 cgtcgatcag ctccagcgcc gggatcacat agtccgtggc gttgtaaacg tcgaacagcg
    11341 tgcagtttgg cccacgcagc ggttttgcca gcacaaaagc cagctccact tcaatgcgcg
    11401 gcacgataaa gcgatcggtc gggatatcgc tgccatcgtg gaagaacatg tcgtccagca
    11461 gtgcaccgta atccggttcg ctgatctgcg agcttgcctg catcgctttc gaggtcagac
    11521 cgattttgtg gcctttcagc gtgcgacctt cggcgatttt caggcgaacc cattcgcgct
    11581 gcacggcgta ggcgtcttcg atggtgatct ccgggtaatc cagcgagatc gcgcggatct
    11641 gttcgcgctg tttttctgcc tgatccagac gctgggcgat cagggtgtgg gtgtgtttat
    11701 cgaacatagc gatatcctgt ggcattgccg gatgcggcag cgccttatca tccggcctgg
    11761 aagctggtgc aaatttattt aaacaacgcg tgtacgttgt tctgtttgaa attcaacgtc
    11821 gggtgcagct ctgcaatctc aaatgaaagc gctaggtaac ggccctccat cagtgaggcg
    11881 aagtgcgttt taatcagatc aaacagcatt tcgcccactt cctgacggct ttccagactg
    11941 cgtccgctgc cgatttttag cgtcatatgc acaaaggcgt aatcgtgctt gccatcagcc
    12001 atttgccagg tatcgatcca gtgcgcccgg ctacgtattc ccccaagcgg aaaaatcccc
    12061 gttgccgcca gtgcagtgtt aacactggca aacagctcag gtaagcgggc ttcttcacga
    12121 atattttcag tacattccac gataaagtgc ggcataaaac gtccttacgc gggcagcggg
    12181 aaaacagcgt taacctgacc ggtgccagag cttgggaaca gctcggtaat aaactccacc
    12241 ttgccgtcgt atttatccca gccgagcatc cccagcagca tcaccgtgtc gtgcatattg
    12301 ccttcgccgt agcagtagtc ggcgtactcc ggcagcatat tgcaaaactc tttgaactgg
    12361 ccttcgcgcc acagcttcac cacacgctcg tccatctggc ggtcgaactc gcgggtgtag
    12421 ctgttcatcc cttcttccgc acgctgatcg tcaataaagc ggtgcgataa cgaaccgctg
    12481 gcaaggaccg ccacggtgcc gtcgtactgt tcgatcgctt tcagaatcgc ttcgcccagc
    12541 ttgcggctgt cggcaaaatc gtgaaccgtg cagaaagccg aaatggagac cactttgaag
    12601 tgcttgtctt cattcatgta gcgcatcggt accagcgtgc cgtattccag tttcaggctg
    12661 ggaatattgt gcgcttttgc ccgcacgccg agctttaagg cttcctcggc aattagctgc
    12721 cccaactccg ggttgccctc gtagttgtag gtcatgtcgc gaataaaatg cggcagctcg
    12781 ttgctggtgt agacgccttc aaaatggtct gcacagttga tgtgataagc actgttgacc
    12841 agccagtgag tatcgaaaac gataatggta tcgacgccca tttcccggca acgcttgctg
    12901 atctctttat gcccgtcgat cgcaccctgg cgacaaccgt ggttttttcc tggtagttca
    12961 gagagataca tcgacggtac gtgcgtgatc ttggcggcta acgctaactt acccatatca
    13021 gactccccat ttcggaattg gatggtcgcc catggaaatg cagacgttct tcatttccgc
    13081 gaacacttcg aagctgtact caccgccttc acgcccggtg ccggaggctt ttacgccgcc
    13141 aaatggctgg cgcaggtcgc gcacgttctg ggtgttgacg aacaccatgc ctgcttcaat
    13201 gccacgcgcc aggcgtaaca ctttgctgac atcctgtgtc cagatgtacg acgcgaggcc
    13261 gtactccacg tcgtttgcca ggcgtaagcc ttcggcttcg tctttaaacg gcagcaggca
    13321 ggcgaccggc ccgaaaatct cttcctgggc aacgcgcata cggttatcta cgtccgccag
    13381 caccgttggg cgcaggaagt tgccgccttt caggtgcgca ggcaggtcgg acggtttatc
    13441 cgggccgccc gccaacaggg ttgcgccttc ttcaatgccg agacggatat agccggagac
    13501 tttttcccag tgttgctgac tgataagcgc cctaacctgg gtattcggat cgttcggatc
    13561 gcccacgcgc agacggttgg cgcgttcggc aaagcgttta acgaattctg gatagatgct
    13621 ctgttgaata aagatgcgcg aaccggcggt gcagcgttcg ccgttgattg agaagatggt
    13681 gaacagggcg gcgtccagcg cgcgctcaat atcggcatct tcaaaaatca gcaccggtga
    13741 tttaccgccc agctccatgg agtatttttt cagcccggcg tttttcatga tgttgcgccc
    13801 ggtggtggta ccgccggtga acgacacggc acgcacgtca tgatgacgga ccagcgcatc
    13861 gcctgcggtt gcgccgtagc cctgtaccac gtttagcacg cccgccggaa taccagcttc
    13921 cagcgccagc tcacccaggc ggtcggcggt cagcggggag agttccgaca tcttcagcac
    13981 cgcggtattg cccagcgcca gacacggcgc gaccttccag gtggcggtca taaacggcac
    14041 gttccacggc gacaccagcg cacaaacgcc taccggttgc accagcgtgt agttgagcat
    14101 cttgtcgtca accggatagg tcttgccgtt catctgctgg cagacttccg cgaaaaattc
    14161 aaagttgtga gaagcgcgtg ggatcaacac atttttggtc tgatggatcg gcaggcccgt
    14221 gtccgcggtt tccatcgcgg cgatctcagg cacgttctgg tcgatcagat cgcccagacg
    14281 gcgcatcagg cgcgcacgct ctttcatcgg cagatcagcc catttcggga acgcctcttt
    14341 cgctgccgct accgcctgat tgatctccgc ttcaccgcca gaggccacat ccgccagcac
    14401 ttcacccgtt gccggattgg tagtctggaa gtagtcgtta tctgcaacat ttttgccgtt
    14461 gatccaatga tttacttttt tcatttcgct gtttcctcac tcacaattcg gttcaccagg
    14521 cggcccacgc cttctacttc caccactact tcatcgccag gcaccacgtc agataagcct
    14581 tttggtgtgc cggtggcgat catgtcgccc ggatttaggg tcataaattc gcttaagtag
    14641 gcgatcagga agggcacgct gaagatcaga tcggcggtgg tgccttgctg gcgtaactcg
    14701 ccgttgacga aggtgcgaag ggtcagatta tgcgggtccg ggatcgcctc tttcggcacg
    14761 atggttgaaa gcatcggcgt cagtccgtcg cggcttttga cccgcaggtt agggcggtag
    14821 tagttttcca gatagtcgcg aatggcgtag tcgttacaca cggtgtagcc cgcgacataa
    14881 tccatggcat cggcttcgct gacgttacgc gcctgcttgc caataactac caccagctcc
    14941 gcttcgtagt gcatgtattc aatattgttc gggcgcacgg aggtctggtt gtcgccagtg
    15001 agggtattcg gcgctttcag gaacaccagc ggctcttccg gtggcttaaa ttccagttcg
    15061 ctggcgtggt cggcgtagtt caggccgagg gcaaacagcg taccgtgcgg gtgtggcagc
    15121 gttgggaagc tcttgcgcgt ggtcacttca cgttcgtcca ctaccggatt ttccagcggc
    15181 gggaaacctt ctgcgagaac acgcacgcga tcgcctggct gtatttccac gcgcgcctgt
    15241 ggcgtgccga gcagaatagc atcgcctgga ttcagcgtgg caaattcgct cagggcgctc
    15301 agcagctgtg cggcgttacg ttgtaaatcg gcggtattcc agtgatcggc aggacgcccg
    15361 ttgatctcgg tatagatagt cagattatcg acattgctga gagccacggt ttcgccaatg
    15421 gggcagaatc catcacgaca ttttgctttg attgccggac ggtaaaagct ctcttccggc
    15481 aggctgacgt cgttagccag cgcatatccg gcgatgtact ctgccgcatc ttcttcacgt
    15541 actttcgtcg ccgttttccc cacaatcaga gcaacagtcg caccgctcag tactttttca
    15601 ccctgtggaa agggaatcgg ttcaccgcaa ccaatcaccg tattgcgcgg tttaataaac
    15661 cagaccgcag ttttaggcgg ggctttgtag ggagattgct ggaacgcttc ctgccaggca
    15721 tcaagttggc tgcgatggtt caacgctacg gcgaagatag tgcctttcat taccactcct
    15781 cggattcgat catcttaatc attaatatgt taatgattac ttttattccc gattgcgtag
    15841 tttggcaaaa gtaagtgata taaatgtgat ctaattaaca aactatttac atttaaagat
    15901 aataaaatat taaatatcag taagttaata aaatatcatc aattttgata aaatgaagaa
    15961 ttgcgggggc agctcagtcc cggtaacgtg atgattaatt gtttctatat taatgatttt
    16021 gaatttttca ggggaaagat aatgcacgac tcactaacca ttgcgttgct ccaggcgcga
    16081 gaagcggcga tgagttattt tcgcccaatc gttaaacggc ataatttgac tgagcaacag
    16141 tggcgcatcg tgcgtattct ggcggaaagc acatcaatgg attttcacga tctggcgtat
    16201 cgcgcctgca ttttgcgccc aagcctgacc gggatcctca cgcggatgga acgcgacggt
    16261 ttagtgttgc gattaaagcc gatcaatgat cagcggaagc tctatatctc gctgaccaaa
    16321 gaggggcagg cgctgtataa ccgcgctcag acacagattg aagaagccta tcggcagatt
    16381 gaagctcagt ttactgccga gaaaatgcaa cagttaaccc atctgttaga ggaatttatt
    16441 gctctgggca attcccgaca agaagacatc ccgggcgata atgaataact ttttagtatc
    16501 tgataaccag cgcctggttt ttccatggat ggcgggttac accttttcat aaagtttttg
    16561 ctttccaggc cgaaaatctt gcatcggtcc acaggaaaga gaaaccatgt taaaacgtat
    16621 caagatcgtg accagcttac tgctggtttt ggccgttttt ggccttttac aactgacatc
    16681 aggcggtctg ttttttaatg ccttaaagaa tgacaaagaa aatttcactg ttttacaaac
    16741 cattcgccag cagcaatcca cgctgaatgg cagctgggtc gcgttgttgc agacgcgtaa
    16801 caccctcaac cgcgcgggta tccgctacat gatggatcag aataatattg gtagcggttc
    16861 aaccgttgct gagctgatgc agagtgccag tatttcgctg aaacaggcgg aaaaaaactg
    16921 ggcggattac gaagcgttgc cgcgtgaccc gtgtcagagc accgccgcag cggcagagat
    16981 caaacgtaat tacgatattt atcacaatgc gctggcggag ctgatccaac tgttaggtgc
    17041 aggcaaaatc aacgagttct ttgatcagcc gacccaggga tatcaggacg gtttcgagaa
    17101 gcagtatgtg gcttacatgg agcaaaacga tcggctctat gatatcgccg tcagcgataa
    17161 caatgcctcc tacagccagg cgatgtggat tctggtgggc gtgatgatcg tcgtactggc
    17221 ggtcatcttc gccgtctggt tcggtattaa agcctcgctg gtagcgccaa tgaatcgcct
    17281 gattgacagc attcgtcata ttgcaggcgg cgatctggtg aaaccgattg aggtggatgg
    17341 ctctaatgag atggggcaac tggcagagag tttgcgccat atgcagggag agctgatgcg
    17401 taccgtcggt gatgtgcgca acggggccaa tgccatctat agcggtgcca gcgaaatcgc
    17461 taccggcaat aatgatctct cttcgcgcac cgagcaacag gccgcttcgc tggaagagac
    17521 ggcagccagc atggagcaac tgaccgcaac ggtgaaacag aacgccgaga atgcgcgcca
    17581 ggccagccat ctggcattaa gtgcttctga aacggcgcaa cgcggcggca aagtggtaga
    17641 taacgtggtg cagactatgc gcgatatctc caccagttcg cagaaaatcg ccgatattat
    17701 cagcgtaatt gacggcatta ccttccagac caatattctg gctttgaacg cggcggttga
    17761 agcagcgcgt gcgggtgagc aaggacgtgg ttttgcggtg gttgcgggag aagtgcgtaa
    17821 tctggcccag cgtagcgccc aggcggctcg tgaaattaaa agcctgattg aagactcggt
    17881 ggggaaagtg gatgttggct ctacgctggt cgaaagcgcc ggggaaacaa tggcggagat
    17941 tgtcagtgct gtgacccgcg tgacggacat tatgggcgaa atagcttctg cttctgatga
    18001 gcagagccgt ggtatcgatc aggttggctt agcggttgct gagatggacc gggtaactca
    18061 acagaacgcc gcgctggtgg aagaatctgc cgctgccgcc gccgcgctgg aagagcaggc
    18121 cagtcgcctg accgaagctg tggcagtgtt ccggattcag caacagcagc aacagcagcg
    18181 tgaaacatcg gctgtggtaa aaaccgtgac gccagctacg ccgcgtaaaa tggcagtggc
    18241 agatagcggg gagaactggg aaacgtttta atcgccataa aaatgcccga taagcaaaat
    18301 gttatcgggc atagggagtt taatctttac gcgggtcgtt gatcggctgg cgaaccagga
    18361 agatgtacgc cagtgctccc gctgcggtga cgcaaccgca aatgattagt gccagacgga
    18421 atgagtgggt tgtatcaaca ataaaaccag taatgatcgg cgcgaaagag gcgcagatga
    18481 agctggcaaa gttctggata ctgcccaccg acgcagtcat gcgagaagca actgcgacgt
    18541 ggatcaagcc ccagcaggat gttccggcaa agtgaataca gaacagtgcc atgccaatca
    18601 gcagaaccgc catcatggat gttgtcgctt gtggtactat cagcgtaaag gcggcagaac
    18661 agaacatccc ggtaataatg cagatcttac ggcttttaat cggagccatt ccccctttga
    18721 ccagccagtc agtaacgtaa ccgttgacca gcatcccggc agccccaaac aggaaaggga
    18781 tagccgccat caaccctgtg ctttttaaat ctaggttata ggctgtttgc aggtaaccag
    18841 gaagccaggc cagatacagc cacgcagtgt agttgatgcc actgaatccg agcatcattc
    18901 cccacattgt gcggttacgg aacaggctgc gccattcggc aaaattgagc ggatctcggc
    18961 gggcattgac gctacctgca ttgagataag cttgttcaac ggcagtcagt tctacgtgct
    19021 cgcggttgcg atagagcata taccagccga tggcgagaaa aatccccagt acaccaatgg
    19081 taataaacat cccgcgccag cccatcacca gcatcattgc cgccagaatc ggtgggctta
    19141 cagcaacgcc aatggtagaa gctgcgttga agaagcccat cgggcgtccg cgctctttga
    19201 tgttgaacca gtcgttaatg acttttacac cgcatgggtt catcggcgct tcaccaatcc
    19261 ccataccgat acgcaccaac acgaactgcg taaagctgtg caccatgcca gacatggcct
    19321 ggaacagtga ccagaagaac atccccagtc ccagcatcag gcgtgggcct ttacgatcca
    19381 atagtgggcc gcaaggaagt tgcgcaatgc cgtaagcgag tgaaaacacg gagagcaaag
    19441 cgccgatttc ggtggcactt aatcccaatt tttcacgaat cgttaaattt gctaccgaca
    19501 gcgaactgcg gtcgagataa ttgattaccg ccgcaaaaaa taataacaac atggcggtgg
    19561 tttgaatgcg tttaattcgg atactgcgtt gaactaatcc atctggtggc acgggaatat
    19621 ctgccgacga agatggagta aatgaagaac gcggatcgag ggtgatattt tctttttcca
    19681 cgccggactc ctgtatttat aattttaatt gtttacgtaa ttgtgcatta cgcaaggttg
    19741 acattaacgg caggttaaag ttttaggact ttactaccgc tcacaaaaac atttttagtc
    19801 gactgattgt gagcataggt aagcgggaat tttccggtat ttatcttttg ataaaaccgt
    19861 gcacccgcta atagtttgct gtttttaatc cgtacttaat gagcataacg cgtgttctca
    19921 ttaatggatc gaatcattga ttgtttagct gaattcaaat ggttacggag
//
LOCUS       DADLSE010000071        19838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLSE010000071 DADLSE010000000
VERSION     DADLSE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.071"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..441
                     /locus_tag="JN692_002460"
     CDS             <1..441
                     /locus_tag="JN692_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HAY7264746.1"
                     /translation="DHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGL
                     AVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQRVC
                     ASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN692_002461"
     CDS             complement(549..2087)
                     /gene="emrY"
                     /locus_tag="JN692_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7264747.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAICYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN692_002462"
     CDS             complement(2087..3250)
                     /gene="emrK"
                     /locus_tag="JN692_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7264748.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3666..4280
                     /gene="evgA"
                     /locus_tag="JN692_002463"
     CDS             3666..4280
                     /gene="evgA"
                     /locus_tag="JN692_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7264749.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4285..7878
                     /gene="evgS"
                     /locus_tag="JN692_002464"
     CDS             4285..7878
                     /gene="evgS"
                     /locus_tag="JN692_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7264750.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNNYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPSVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFKIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN692_002465"
     CDS             complement(7934..9079)
                     /gene="yfdE"
                     /locus_tag="JN692_002465"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7264751.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN692_002466"
     CDS             complement(9153..10097)
                     /gene="yfdV"
                     /locus_tag="JN692_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7264752.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLEPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN692_002467"
     CDS             complement(10167..11861)
                     /gene="oxc"
                     /locus_tag="JN692_002467"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7264753.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN692_002468"
     CDS             complement(11915..13165)
                     /gene="frc"
                     /locus_tag="JN692_002468"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7264754.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(13678..14301)
                     /locus_tag="JN692_002469"
     CDS             complement(13678..14301)
                     /locus_tag="JN692_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7264755.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            14598..14873
                     /locus_tag="JN692_002470"
     CDS             14598..14873
                     /locus_tag="JN692_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264756.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(14950..15192)
                     /locus_tag="JN692_002471"
     CDS             complement(14950..15192)
                     /locus_tag="JN692_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7264757.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            15545..16465
                     /gene="lpxP"
                     /locus_tag="JN692_002472"
     CDS             15545..16465
                     /gene="lpxP"
                     /locus_tag="JN692_002472"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7264758.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMLGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTIVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            16820..16891
                     /gene="ypdK"
                     /locus_tag="JN692_002473"
     CDS             16820..16891
                     /gene="ypdK"
                     /locus_tag="JN692_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7264759.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN692_002474"
     CDS             complement(16956..18194)
                     /gene="alaC"
                     /locus_tag="JN692_002474"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7264760.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            18571..>19838
                     /locus_tag="JN692_002475"
     CDS             18571..>19838
                     /locus_tag="JN692_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7264761.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQ"
BASE COUNT         5628 a         4591 c         4220 g         5399 t
ORIGIN      
        1 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
       61 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
      121 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
      181 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
      241 caggaagaag gtattcgtct tgaaccttca gcactggcgg gtatggccgg acctcagcgc
      301 gtgtgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca attacgtaat
      361 gccactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
      421 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctaggtg
      481 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
      541 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
      601 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
      661 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accatagtat
      721 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
      781 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
      841 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
      901 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
      961 ccctggaaaa actgtggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     1021 gaacgccagt aatagcaaat cgcatacatc aaaaaactga atgtcactaa cacccgcatg
     1081 tctattttat tgccataacg tcctatcaaa ggtgaaatca atagtggcat gatgccgatg
     1141 ggcgcataag caagcccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     1201 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc aggtgatact cacaatacca
     1261 atggtgaaat tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     1321 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     1381 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     1441 ccgagcacta acagggtcag tcctggtaga ttcattttta ccggtgaagt ttcagtttct
     1501 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     1561 aaaaatatcc aaccccagct aaagttatcg caaatatagc cgcccaatat cggcccacat
     1621 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggt
     1681 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaatccc
     1741 tgaacgactc taaaaaatat cagcacatca agattggtcg ataaactaca cattaaggaa
     1801 gacagcgaaa aaaaagtgac tgaaagtaaa aataacctta attcgcctat tctttgtgcc
     1861 agcctgccgg taacagggat cgcaatggca tttgctacgc caaacgaggt gataacccag
     1921 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     1981 atagtggaat ccaacatttg cataaacgtc gctaatgaca atgcaatagt aacgcaccat
     2041 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     2101 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     2161 tactggtata agcaggcatg gaggtcacgg ttgaagccag atcaggcatc tcagcaatgt
     2221 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     2281 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     2341 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     2401 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     2461 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     2521 gacgtgccgg tacgacagcc attaacgatt gtccggaact cactgtttcg ccgacctgaa
     2581 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcacca
     2641 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     2701 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     2761 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     2821 tcgctaaagg cacacgacgg ttataatctt ctaaagattg ttgatactga atacgtgctg
     2881 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     2941 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     3001 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     3061 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     3121 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     3181 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     3241 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     3301 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     3361 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     3421 caattcttac gcctgtagga ttagtgagaa gacttatagt gctaacttga aactataaat
     3481 catcggtata atccctgatt ttattgttga catttcattt atgccgacta tttatatggt
     3541 atacttgtcg aattatctta aaggaagctc agattttctt atttttattg agaaaatgag
     3601 atgacgcctt atgtctgtat tactacaggg agaagggaga tgcttcattg caaagggaat
     3661 aatctatgaa cgcaataatt attgatgacc atcctcttgc tatcgcagca attcgtaatt
     3721 tattgatcaa aaacgatatt gaaatcttag cggagttaac tgaaggcgga agcgccgttc
     3781 agcgggtgga aacacttaag cctgatatcg tcatcattga tgtcgatatc cccggagtta
     3841 acggtatcca ggtgttagaa acgctgagga agcgccaata tagcggaatt attattatcg
     3901 tctccgctaa aaatgaccat ttttacggga aacattgtgc tgatgctggc gctaatggtt
     3961 tcgtgagtaa aaaagaaggc atgaacaata tcattgcggc tattgaagct gcaaaaaatg
     4021 gctactgcta tttccccttc tctctcaacc ggtttgttgg aagtttaacg tccgaccagc
     4081 aaaaactcga ctccttatcg aaacaagaaa ttagtgtcat gcggtatatt cttgatggca
     4141 aggataataa tgacattgcg gaaaaaatgt tcatcagcaa caaaactgtc agcacgtata
     4201 aaagtcgcct gatggaaaaa ttagaatgta aatcactgat ggatctttac acattcgcgc
     4261 aacgtaacaa aatcggctaa tcacatgaag tttttaccct atttttttct tctctgttgt
     4321 ggtctttggt cgaccataag tttcgcagac gaagattaca tcgaatatcg tggcatcagt
     4381 agtaacaacc gtgtcacact tgatccagta cgtctgagca acaaggaatt acgttggtta
     4441 gcgagcaaaa aaaatcttgt gattgcagta cacaagtccc aaacggctac cttgttgcat
     4501 accgattcgc agcaacaggt tcgtggtatt aatgctgatt atttaaatct tttaaaaaga
     4561 gcgttaaata tcaaattaac actccgggaa tacgcagatc atcaaaaagc gatggacgcg
     4621 cttgcagaag gtgaagtcga tatagtgtta tcacatttag ttacttcgcc gcctcttaat
     4681 aatgacattg ctgcaaccaa accattgata attacctttc cggcgctggt aaccaccctt
     4741 cacgactcaa tgcgaccgct tacctcacca aaaccagtaa atattgctcg ggtagcaaat
     4801 taccccccag acgaggtaat tcatcaatca tttccaaaag caacaattat ctcttttaca
     4861 aatttatatc aggcattagc atccgtctca gctgggcaca ataattactt tattggtagt
     4921 aacatcatta ccagcagtat gatttcccgc tatttcactc actccttaaa tgtagtgaaa
     4981 tattataact cgccgcgtca atataatttt ttcttgacca gaaaagaatc tgtcattctt
     5041 aatgaagtac tcaatagatt tgttgatgct ttaacaaatg aagttcgcta tgaagtatca
     5101 caaaattggc ttgatacagg aaacctggcc tttctgaaca aaccattaga actcactgaa
     5161 catgaaaaac agtggattaa gcagcatccc aatttaaagg tgctggaaaa tccttactcg
     5221 cccccctatt ctatgacgga tgaaaatggc tcggttcggg gcgttatggg ggacattctt
     5281 aatattatta ccttgcaaac aggtttaaat ttttctccga tcaccgtttc acacaatatc
     5341 catgctggaa cacagcttag ccccggagga tgggatataa tacctggcgc tatttatagt
     5401 gaagatcgag aaaataatgt tttatttgct gaagccttca taacaacgcc ttacgttttt
     5461 gtcatgcaaa aagcgcctga cagtgaacaa acattaaaaa aaggaatgaa agttgccatt
     5521 ccatattatt atgagctgca ttcgcaatta aaagagatgt atccggaggt tgaatggata
     5581 caggtcgata atgccagcgc tgcatttcac aaggttaagg aaggtgaact tgatgctctg
     5641 gtcgcgacac agctaaattc gcgttacatg atcgatcatt actatcctaa tgaactttat
     5701 cattttctta ttcctagcgt tccgaatgca tcgctttcgt tcgcttttcc tcgcggagaa
     5761 ccggaactta aggatattat taataaagca ctgaatgcaa ttcccccaag cgaagttctg
     5821 cgcctgacgg aaaaatggat taaaatgccc aatgtgacca ttgacacatg ggacctatat
     5881 agcgagcaat tttatattgt tacgacatta tccgttttat tagttggcag tagcctttta
     5941 tggggattct acctgttacg ctcagttcgt cgtcgtaaag tcattcaggg tgatttagaa
     6001 aaccaaatat cattccgaaa agcactctcg gattccttac cgaatccaac ttatgttgta
     6061 aactggcaag gtaatgtcat tagtcataat agtgcttttg aacattattt cactgcggat
     6121 tactacaaaa atgcaatgtt accattagaa aacagtgact caccctttaa agatgttttt
     6181 tctaatgcgc atgaagtcac agcagaaacg aaagaaaatc gaacaatata cacacaggta
     6241 tttaaaattg ataatggcat cgagaaaaga tgcattaatc actggcatac attatgcaat
     6301 cttcctgcaa gtgacaatgc agtatatatt tgtggttggc aagatattac tgaaacgcgt
     6361 gatctaatta atgcactcga ggtagaaaaa aataaagcga taaaggctac cgtagcaaaa
     6421 agtcagtttc tggcaacgat gagtcacgaa ataagaacac caataagctc tattatgggc
     6481 ttcctggaac ttctgtcggg ttctggtctt agcaaggagc aacgggtgga ggcgatttca
     6541 cttgcctacg ccaccggaca atcactcctc ggcttaattg gtgaaatcct tgatgtcgac
     6601 aaaattgaat cgggtaacta tcaacttcaa ccacaatggg tcgatatccc tactttagtc
     6661 cagaacactt gtcactcttt cggtgcgatt gctgcaagca aatcgatcgc attaagttgc
     6721 agcagtacat ttcctgatca ttacctggtt aagatcgacc ctcaggcgtt taagcaggtc
     6781 ttatcaaatt tactgagtaa tgctctcaaa tttaccaccg agggggcagt aaaaattacg
     6841 acctccctgg gtcacattga tgacaaccac gctgttatca aaatgacgat tatggattct
     6901 ggaagtggat tatcgcagga agaacaacaa caactgttta aacgctacag ccaaacaagt
     6961 gcaggtcgtc agcaaacagg ttctggttta ggcttaatga tctgcaaaga attaattaaa
     7021 aatatgcagg gcgatttatc attagaaagt catccaggca taggaacaac atttacgatc
     7081 acaatcccgg tagaaattag ccagcaagtg gcgactgtcg aggcaaaagc agaacaaccc
     7141 atcacactac ctgaaaagtt gagcatatta atcgcggatg atcatccgac caacaggcta
     7201 ttactcaaac gccagctaaa tctattagga tatgatgttg atgaagccac tgatggtgtg
     7261 caagcgctac acaaagtcag tatgcaacat tatgatctgc ttattactga cgttaatatg
     7321 ccgaatatgg atggttttga gttgactcgc aaactccgtg agcaaaattc ttccttaccc
     7381 atctgggggc ttacagccaa cgcacaggct aacgaacgtg aaaaagggtt aagttgcggc
     7441 atgaacttat gtttgttcaa accgttgacc ctggatgtac tgaaaacaca tttaagtcag
     7501 ttacaccagg ttgcgcatat tgcacctcag tatcgccacc ttgatatcga agccctgaaa
     7561 aataatacgg cgaatgatct acaactgatg caggagattc tcatgacttt ccagcatgaa
     7621 acgcataaag atctacccgc tgcgtttcat gcactagaag ctggcgataa cagaactttc
     7681 catcagtgta ttcatcgcat ccacggtgcg gctaacatcc tgaatttgca aaagttgatt
     7741 aatattagcc atcagttaga aataacacct gcttcagatg acagtaagcc tgaaattctt
     7801 cagctgctga actctgttaa agaacacatt gcagagctgg accaggagat tgctgttttc
     7861 tgtcagaaaa atgactaaat tgcggctccc acaatgttct aatgtgggag ctatttacca
     7921 gcacatctta catttatgat gagaactcct ggcgaatttg ttccccatgc tggtcgagcg
     7981 ttgccgctcc cggcataaca tgcgggtccg cgcagccgct gatttttatc ggattacccg
     8041 gcatcattat tcctccggct tcaatcaaca tatttctcgc ctgagtttgt ggcaaattaa
     8101 tggcctcagc cacacttaat aacggcgcga cgggcacacc aacttcatgt attttggcta
     8161 accaaacttc agctgcctgc gtttttaacg ttcgctcaat atattgttta agaatagcct
     8221 ggttttgtac gcgtaaaata ttgctgctaa atcggggatc attaaccagt tccgtaagct
     8281 ccagtgcctg gcataacgca gaaaaaagct tgtcattacc acaacaaatc gtaatcggct
     8341 tatcctgagt atcgaaaaca tcaaaaggtg ccatgtaggg atggcgattt cccaggcgtt
     8401 gtggtgactt ccctgtcgca atatatgcca tcagaccatg ctccagaaaa ctcagcgtgg
     8461 catcaaacat cgctatatcg acatgcgccc ctctctggct cttttcgcgg ccataaagtg
     8521 cactcactat tccgctgaat aaatagacac cgccgcatag atccgcaaga gaggtaccaa
     8581 cacgcactgg cggagcatca gggtaccctg tttccatcat tatcccactc attgcctgaa
     8641 tgatggtatc gtaggcagga gcatctttta gcggaccggt atgtccgaaa cctgacgatg
     8701 aagcatatat gaggcgcggg ttgatttctt gtaacctttc ccatgaaaac cccagttttt
     8761 ccattgtacc tgggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
     8821 atatactttt atcatgatca ttctttaaat caagaaccac actctctttg ccatgattaa
     8881 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
     8941 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
     9001 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
     9061 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
     9121 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
     9181 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
     9241 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
     9301 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
     9361 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
     9421 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
     9481 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
     9541 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
     9601 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
     9661 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
     9721 gatacggaat caccataaat aggctcgaga actgcaaacc ccaagaatcc aatggtaggt
     9781 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
     9841 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
     9901 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
     9961 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    10021 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    10081 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    10141 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    10201 gtaatatggc cactttcagt tcctgctgca gggtcgatga ccacattaat aatggtcggt
    10261 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    10321 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    10381 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    10441 ttattaaaaa taacgatcgt caccggcagg ttatatcgac aaatcgtttc aatttccatc
    10501 ccactgaaac caaaagcact atcaccttca atggcgacaa ccggagaacc agaagtcacg
    10561 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    10621 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    10681 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    10741 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    10801 tgctgcttgt ggatatttaa aatatcgcgc cataccagtg gagtcgtaaa tgtgttttgt
    10861 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    10921 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    10981 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    11041 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    11101 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    11161 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    11221 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    11281 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    11341 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    11401 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    11461 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    11521 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    11581 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    11641 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    11701 tcatgacgaa aaccaatata acgaatgcct tccgcctggg catggcgtgc catatccgtc
    11761 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    11821 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    11881 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    11941 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    12001 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    12061 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    12121 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    12181 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    12241 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    12301 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    12361 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    12421 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    12481 gcgggtattc ttccagataa cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    12541 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    12601 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    12661 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    12721 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    12781 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    12841 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    12901 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    12961 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    13021 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    13081 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    13141 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    13201 ttagcgttta tttaaggcat aacgccctct ataaataatc tgcaaaatat cttcgtagcg
    13261 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gccacttcca
    13321 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    13381 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    13441 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    13501 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    13561 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    13621 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    13681 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    13741 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    13801 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    13861 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    13921 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    13981 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    14041 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    14101 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    14161 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    14221 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    14281 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    14341 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    14401 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgct
    14461 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    14521 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    14581 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    14641 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    14701 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    14761 cacaggcgat tttgtttaag atgagagaag atgttccacc attaaacctc acagaagaaa
    14821 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgcga tagcgtctgt
    14881 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    14941 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    15001 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    15061 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    15121 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    15181 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    15241 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    15301 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    15361 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    15421 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    15481 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    15541 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    15601 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    15661 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    15721 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    15781 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    15841 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    15901 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    15961 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    16021 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgctcggcag
    16081 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    16141 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    16201 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    16261 aacgatagta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    16321 aggctacccg acagatgaaa atcaagccgc tgcctatatg aacaagatta tcgagaaaga
    16381 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    16441 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    16501 cataataacc tcatgatttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    16561 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    16621 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    16681 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacagggct tttaaggtcg
    16741 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    16801 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggttatcgt
    16861 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    16921 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    16981 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    17041 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    17101 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    17161 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    17221 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    17281 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    17341 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    17401 gcactgacca gcgttttgtt gccgaccata aagccgatac gccagcccgc catgttgtag
    17461 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    17521 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    17581 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    17641 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    17701 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    17761 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    17821 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    17881 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    17941 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    18001 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    18061 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    18121 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    18181 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    18241 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    18301 aacattttta tcaatggctt taaatgagga actccacttc aaaaacagga aatttttcta
    18361 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagt
    18421 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    18481 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    18541 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    18601 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    18661 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    18721 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    18781 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    18841 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    18901 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    18961 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    19021 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    19081 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    19141 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    19201 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    19261 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    19321 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    19381 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    19441 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    19501 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    19561 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    19621 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    19681 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    19741 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    19801 gatgagcaaa tcgatatcaa aaaagagctg tatcaaat
//
LOCUS       DADLSE010000072        19697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLSE010000072 DADLSE010000000
VERSION     DADLSE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.072"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="JN692_002476"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="JN692_002476"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7264762.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1542..2348
                     /gene="tcdA"
                     /locus_tag="JN692_002477"
     CDS             1542..2348
                     /gene="tcdA"
                     /locus_tag="JN692_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7264763.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN692_002478"
     CDS             complement(2399..2842)
                     /gene="csdE"
                     /locus_tag="JN692_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7264764.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN692_002479"
     CDS             complement(2842..4047)
                     /gene="csdA"
                     /locus_tag="JN692_002479"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7264765.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4239..4466
                     /locus_tag="JN692_002480"
     CDS             4239..4466
                     /locus_tag="JN692_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7264766.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4817..5734
                     /locus_tag="JN692_002481"
     CDS             4817..5734
                     /locus_tag="JN692_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7264767.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5753..6148
                     /locus_tag="JN692_002482"
     CDS             5753..6148
                     /locus_tag="JN692_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7264768.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6141..7241
                     /gene="rlmM"
                     /locus_tag="JN692_002483"
     CDS             6141..7241
                     /gene="rlmM"
                     /locus_tag="JN692_002483"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7264769.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN692_002484"
     CDS             complement(7285..8016)
                     /gene="fucR"
                     /locus_tag="JN692_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7264770.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN692_002485"
     CDS             complement(8074..8496)
                     /gene="fucU"
                     /locus_tag="JN692_002485"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7264771.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN692_002486"
     CDS             complement(8498..9916)
                     /gene="fucK"
                     /locus_tag="JN692_002486"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HAY7264772.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVNKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAWLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     YELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN692_002487"
                     /pseudo
     CDS             complement(10025..11800)
                     /gene="fucI"
                     /locus_tag="JN692_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN692_002488"
     CDS             complement(11833..13149)
                     /gene="fucP"
                     /locus_tag="JN692_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7264773.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13696..14343
                     /gene="fucA"
                     /locus_tag="JN692_002489"
     CDS             13696..14343
                     /gene="fucA"
                     /locus_tag="JN692_002489"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7264774.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14371..15519
                     /gene="fucO"
                     /locus_tag="JN692_002490"
     CDS             14371..15519
                     /gene="fucO"
                     /locus_tag="JN692_002490"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7264775.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN692_002491"
     CDS             complement(15572..16417)
                     /gene="xni"
                     /locus_tag="JN692_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7264776.1"
                     /translation="MRGLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN692_002492"
     CDS             complement(16438..17805)
                     /gene="sdaB"
                     /locus_tag="JN692_002492"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7264777.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17863..19152)
                     /locus_tag="JN692_002493"
     CDS             complement(17863..19152)
                     /locus_tag="JN692_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7264778.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4865 a         4826 c         5196 g         4810 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgccgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcgg gggcattggg cgataaatac atcctcgctt acagcaactc
      721 tctgatggat aacttcatta tggatgtgca gggtagtggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ttggatgtcg ggggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gggtttgggt
     1261 gctgaaaacg gctccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcacat cctgattatt gactaagtgc
     1381 gcgttgttca taccggatgc ggcgtgaacg ccttatccgg cctacaaatc atgcaaatcc
     1441 aaaatattgc aggagttatg taggcctgat aagcgtagcg catcaggcat ttattcgttt
     1501 agggtgaggg ataacctcgc cctttttaat tctgaggtgc tatgtctgtg gtaattagtg
     1561 atgcatggcg tcagcgtttt ggtggcacag cgcgtctgta tggtgaaaaa gcgttgcaac
     1621 tgtttgctga cgcgcatatt tgtgtggttg gtatcggtgg tgtcggttcc tgggcggcgg
     1681 aagcgctggc gcgcacgggg attggcgcaa tcacgcttat cgatatggat gatgtgtgcg
     1741 tcaccaatac caatcggcaa attcacgctc tgcgcgataa cgtcgggctg gcaaaagcgg
     1801 aagtcatggc ggagcgcatt cgccagatta acccggagtg ccgtgtaacg gtggtggatg
     1861 atttcgtgac gccggataac gtagcgcaat atatgagtgc cggttattcg tacgtgattg
     1921 atgccattga tagtgtacgg cccaaagcgg cgctgattgc ttattgtcgg cgcaataaaa
     1981 tcccgctggt tacgaccggt ggcgcgggtg ggcagattga cccgacgcag attcaggtta
     2041 ccgatctggc gaaaacgatt caggatccgc tggcggcgaa gttgcgcgag cgcctgaaaa
     2101 gcgattttgg cgtagtgaaa aacagtaaag gtaagctcgg cgtggattgc gtgttttcta
     2161 ctgaagcgct ggtgtacccg cagtcagacg gtacggtgtg tgcgatgaaa gccacggcag
     2221 aagggccgaa gcggatggat tgtgcatctg gatttggcgc ggcaacgatg gtgaccgcca
     2281 cctttgggtt tgttgcggtt tctcatgcgc tgaagaagat gatggcgaaa gcggcgcgtc
     2341 agggttaaac aatcaggtcg gatgagacgc gaacgtctta tccgacccgg ttctgtgctt
     2401 aaacctgctt cgtcgcagcg ataatcgcct cgcttaacgc atttaacccc tggctgcgtg
     2461 aggcgctaag ctgcgcacgt aatcccagct catcaaacaa tgccagcggc gactgtgcct
     2521 gtaattcggc ggcggttttc ccctcaacgg cagtcaacaa caccgccaac aggccgcgca
     2581 caatgcgccc ttcgctgtcg ccaaagaagt gcattttgcc gttttcagcc acggtatatc
     2641 ccagccagac gcggttttcg catccggcaa tctctttagc ctgcgctttt aactcgtctg
     2701 gcaaagccgg aagctgtttc cccagcatga tcaactggcg atatttatct tcccattgcg
     2761 acaacggtgc gaaggtattg cgtaacgttt ctgcggttac ggttgtgccg aacggatgtc
     2821 cggcgaattg cgggtttgtc attaatccac caataattcc agcgcgcggt caacggcatt
     2881 caccagcgca tccacatcac tctttgtatt atatggcgca aatgaggcgc gcagtgtgcc
     2941 ggttacgcct aattctgcca gtagcggctg agcgcaatgt tgcccggccc gcaaggcaat
     3001 accgtactcc gccagtagcg tcaccatatc gctgtgatga acgccagcaa aatcaaaggc
     3061 cagcaggctg gaatcctggc agcggaatga acgaaagcct ggacgtttcg ccagcgcttc
     3121 ttctgcaagc gttgctaagc tacggctcca gctttcggcc tggttgatat cgtaatctgc
     3181 cagccattcc agcgccgcgc ttaatcctat gacgccagcg acatttggcg ttcctgcttc
     3241 cagtttccac ggcgcagatt gagtggtgaa accgtcaaaa ctcacttcgt gaatcatttt
     3301 gccgccaccc agccagggcg acatcgcttc cagcagttct gatttgccat acagcacgcc
     3361 gatacccgtc gggccataca gtttgtgacc agagaaagca tagaaatcga tatccagttg
     3421 ctgaacatcc gcggggaaat gcaccgcccc ctgagcacca tcaaccatca ccaccatccc
     3481 ggctgaatga gcaaaggtaa tcgctcgcgc cagatccggg caaccgccag tcacgttcga
     3541 catctgaccc aacgccagaa tccgactacg gggagtaatc agttctggca acaaatcgac
     3601 atccggcagt cgctgcgcat taagcggcaa tttcaccact ttggctctag tttgttgggc
     3661 gaccatcagc caggggacga ggttagcgtg gtgttctgcc acgctgacaa taatctcatc
     3721 gcccggttgc agacgcggac gcgcatagca ttgtgccacc atgttgatgg attcagtggt
     3781 gccgcgcgtc cagacgatag ttttatcatc cggtgcattc agtaattgcg ccactttctc
     3841 tcgtgcagct tcataacgcg cggtcaggcg ttgggcttcg gcaaactggc tgcgatggac
     3901 gtttccggcg ctcaggctgt aaaactgtcg agtggcttca accacggctt caggtttaag
     3961 cgcggtcgcg gcgctgtcga gatagacgcc cgcatcctgt agtgcgggaa actgggcgcg
     4021 aaactgcgcg ggattaaaaa cgttcatggt actcctcggc ttgatgcacc gatcgtgacg
     4081 caaaattcac tttgatacaa ggagagtgct aaccatcaga attatctgga tatcctggcg
     4141 aagccgcgta agtaacatat gctgaatagt gttaggcgtt aattttagcc tgttaatcaa
     4201 tactattaag atttattaat cgctttaagg agaagatgat gaaaaagact gccgcaatta
     4261 tttctgcctg tatgctgact tttgccctga gcgcctgttc cggttcgaac tatgtgatgc
     4321 acaccaatga cggacgtacc atcgtctctg acggcaaacc acagactgat aacgataccg
     4381 gtatgatttc gtataaagac gctaatggca acaaacagca gatcaaccgt actgacgtga
     4441 aagagatggt cgaactggaa cagtaattcg cgatcgcaag gtaaaaaaaa gcaccgcaat
     4501 taggcggtgc tacattaatc actatggaca gacagggtaa atgtacagga agtgaaaaaa
     4561 ggtagctttg ctaccatggt ctgaatcgca gaccaattgc aaacgcaaca acacaacatc
     4621 acaaccgtaa gccaaaagtt caccagaata cgcattccga taaaactttt cgttccggct
     4681 caggaagtgc cgccactata ggtatttgct ggtagaagct caacggacaa tttataatgg
     4741 ctcagattaa aaaaactaat aggttacaca gtgtgatcta attgttaaat tcatttaaca
     4801 tcaaagttta aaagccatgt ctaaacgatt accaccgcta aatgccttac gagtttttga
     4861 tgccgcagca cgccatttaa gtttcactcg cgcagcagaa gagctttttg tgacccaggc
     4921 cgcagtaagt catcaaatca agtctcttga ggattttttg gggctaaaac tgttccgccg
     4981 ccgtaatcgt tcactcctgc tgaccgagga agggcaaagc tatttcctcg atatcaaaga
     5041 gatattttcg caattaaccg aagcgacgcg taaactccag gcccgtagcg ccaagggggc
     5101 gttgacggtc agtttactcc ccagtttcgc cattcattgg ttggttccgc gactttccag
     5161 ctttaattca gcttatccgg gaattgacgt tcgaatccag gcggttgatc gtcaggaaga
     5221 taagctggcg gatgatgttg atgtggcgat attttatggt cggggcaact ggccggggct
     5281 acgggtggaa aaactgtacg ccgaatattt attgccggtg tgttcgccgc tactgctgac
     5341 aggcgaaaaa cccttgaaga ccccggaaga tctggctaaa catacgttat tacatgatgc
     5401 gtcacgccgt gactggcaga catatacccg acagttgggg ttaaatcata tcaacgttca
     5461 gcaagggcca atttttagtc atagcgccat ggtgctgcaa gcggctattc acgggcaggg
     5521 agtggcgctg gcaaataacg tgatggcgca atctgaaatc gaggccggac gtcttgtttg
     5581 cccgtttaat gatgttctgg tcagtaaaaa tgctttttat ctggtttgtc atgacagcca
     5641 ggcagaactg ggtaaaatag ccgcctttcg ccaatggatc ctggcgaaag ccgctgctga
     5701 acaagaaaaa ttccgctttc gttatgaaca ataatttacg tagggtacga ccatgaccag
     5761 ccgttttatg ctgattttcg ccgccattag cggcttcatt tttgtggcac tgggcgcttt
     5821 tggtgcgcat gtgttaagta aaaccatggg ggccgttgag atgggctgga tccaaaccgg
     5881 tctcgaatac caggcgtttc atacgctggc gatcttaggt ctggcggtgg caatgcagcg
     5941 tcgcatcagt atctggtttt actggagtag cgttttcctc gcgttaggca cggtgctgtt
     6001 cagcggcagc ctttattgcc tggcgctgtc ccatctgcgt ttgtgggcgt ttgtcacacc
     6061 ggttggcggc gtgagcttcc ttgcgggctg ggcgttaatg ttagttggtg ctatccgttt
     6121 aaagcgcaag ggcgtaagtc atgaataagg ttgtattgct gtgccgtccg ggctttgaaa
     6181 aagagtgcgc cgcagaaatt accgataaag ccggccagcg ggaaattttc ggttttgccc
     6241 gcgtgaaaga gaatgcgggt tatgtcattt atgaatgtta tcaacctgat gatggcgata
     6301 agttaatccg tgagctgccg ttcagttcat taatttttgc ccgccagtgg tttgtggtgg
     6361 gggaactcct gcagcatttg ccgccagaag atcgtattac ccccattgtc ggcatgttac
     6421 agggtgtagt agagaagggc ggtgaactgc gtgttgaagt tgccgatacc aacgaaagca
     6481 aagagttgct gaaattctgc cgtaagttca ctgttccgct acgcgctgcc ttgcgcgatg
     6541 ctggggtgct ggcgaactat gaaacgccga agcgtccggt tgtgcatgta ttctttattg
     6601 caccaggatg ctgctatacc ggttactcat acagcaacaa taattcgccg ttctatatgg
     6661 gcattccgcg cctgaaattt ccggcagagg cgccgagtcg ttccacgctc aaactggaag
     6721 aggcatttca tgtgtttatt cctgcggatg agtgggatga acgcctggcg aacgggatgt
     6781 gggcggtgga tttaggcgct tgccctggcg gctggaccta ccaactggtg aagcgcaaca
     6841 tgtgggttta ttccgtcgac aacggcccga tggcgcaaag tctgatggat accgggcagg
     6901 tgacgtggct gcgggaagac ggtttcaaat tccgtccgac acgcagcaat atctcctgga
     6961 tggtatgcga tatggttgaa aaaccggcta aagttgcggc attgatggcg cagtggctgg
     7021 ttaatggctg gtgccgtgaa accattttca atctcaaact gccgatgaaa aaacgctacg
     7081 aagaagtatc acacaatctg gcgtatattc aggcacagct tgatgaacat ggcataaatg
     7141 ctcagattca ggcgcggcag ttgtatcacg atcgcgaaga agtgacggtg cacgtccgcc
     7201 gcatctgggc tgcggtgggt ggtcgtcgcg acgagcgata acaggcaagg ccggggcgtc
     7261 gtctccggcc tgctaccctt tccgtcaggc tgttaccaaa gaagttgcaa cctggcgctc
     7321 atcagaaata atgtgtgtta cctcatccag atgcccgatg cgggcttccc ctgaacgatt
     7381 aaatttactt ttatcaatca ataacaacga ttgcgcggca cgttttaata gcatcgattt
     7441 gtaatcagcg ttgatcgcat tggagtccca cagtgcgccg ctgctatcga tcccttcaca
     7501 agaaaaaata aacagatcga tttccagcga tttaagttgg gaaatcagcg agggattgac
     7561 gtaacagcca tatttgcgct caagtgtgcc gccggaactg atcagttgaa tgcgttcgcg
     7621 tttaccgagt tcatggcaaa tcggatggct attggtgaag acctgaatgt tgatgtcagg
     7681 caactggcgt gccagatacc agcaagttga actggcgtct aaggctatca ccatcccttc
     7741 ttcaatccac gcgagcgcct cgcgcgcgat atctgcttta tgcgcataat ggcttttcag
     7801 cctgatgtga aagggatcgc cactgtcttg attttgacgg tggatatatt tagcgcgtcc
     7861 atgattgcgc agaattttac cctgcgtctg taattcattg agatcgcgac gaatggtttc
     7921 tttactgacc tttagctgtt cagagagagc ttccgtggtc aggctggtat ggttcagcag
     7981 caggtcgact atcgcttgct ggcgtgccgc tttcatattt ttcaccccat cattcgataa
     8041 ccgcccgcgt catgcgggcg taccggtatg agattacggt gttacccctt tttttaaaag
     8101 aatattcccg tacttcgctc gttcgcctgt gataacgatc gcaaaggctt tttgcgcccg
     8161 ttcataaaac gcaaaacgat tgatgcggat gatgtcagga cacggggctt gtagtgaaag
     8221 cgcattacgg taacgtcgtt ctacttcagg atcgagagtg tcaccctcta ccgccgccat
     8281 catcaccagc ggcggggcat aactgtccag ctcaaataac gggataatcg cctggagcaa
     8341 gtcgctcacc aacaggccat cagcgcggat cacctgcggt cccatcgaat gggcgggaaa
     8401 gtgagcatcg gaaaaaataa tttcatctcc atgtcccatc tctgccagca ctttcaatag
     8461 ttcgggagaa attaacggcg aaattgtttt cagcatttca cacttcctct ataaattcag
     8521 gttcagtttg cgggtagaaa taacggtact gataatgaat ctgtgcacgg gcttcttccg
     8581 ggctgttaaa ttcccctacg ccataccaac cgaacagcgc agctcctgcg acggtcgttt
     8641 cggcatcgtc gagaactttt accggaatat caagcatatt ggctttaatc tgattccaca
     8701 atgtgttgcg acttcctcca ccgactaaca ataattcata ggctttaaag tgcccgattt
     8761 tttccagcat ctgtagattg cgctgtaatt gcgcagttaa cccttccagc gccgcgcgat
     8821 agaaatgccc ccgcgtggta ttaagcgtca ctccttgcca gccagcgttc tgacacgaca
     8881 ataaatcaca ctgcattttc acaccatccg cgccaggtgc gatcagacga gcttcttcaa
     8941 tcaacatttg ccagggtgtt tcagccgtcc agaacagttt tctcacccat tccagcacgc
     9001 cggatgccag ccattgcata cctgggttat acaaccatgc ctggctatct agttcgcagg
     9061 tggaaccggc gtactgactt aacagcgaag tatcaacctg ggcgctgcga accattaaaa
     9121 tttcccatgt accggaggag agcacgggtt cattttgttc ggcaccagcg ccaaaaaggg
     9181 cgaactgggt atcgtgacct gcggaaatca ccggtatgcc aacgggtaag ccgagcattg
     9241 ctgcggcgct gttctgtagc gtaccaatct gttcacccgc ttccaccaga cgagggaaga
     9301 gtcggcgtgg aataccggtg gcttgtaaaa tttgcggact gaaatcgcgt tgctggatat
     9361 ccagcatctg gctggttccg gccatcgtga tatcagtagt gaattcgccg gttaaacggt
     9421 ggttaatcag cgacgaaatg aagagccagg cgtgcgcgcg ttccagcagt tgtggatgat
     9481 tttctttcaa ccacaccaac ttatataacg tattgaaact aaaggctccg acgccagaaa
     9541 tagcctgcaa ccgctgtgcg gagattaacc gttcaatatt gtccataacc gctgctgttc
     9601 gcggacattt ccagctaatg atcggataga gcagattgcc ttgcttattt accagagcgc
     9661 catccacacc aaaggtggtg acggcgatac cgcggatgtg gcattcagtc agttcactat
     9721 tgatttgccg acagcaatca gcaaagcgtt gcaaaatggc gtctaaagac cactggtgcc
     9781 aggtgttgtt ttccatcgcg atatcgctgg cattaggcgt tgaggcgcgg gcaacaattt
     9841 ttccctgccg attaaccgcg atggccctga cattggtcgc gccacagtcg agtaccagga
     9901 taacttcttg tttcataatc gctcctgcaa tatagccgga taacattgtt tatccggcta
     9961 accactcttg cttgttcagg cccggtagcc ggagcgaccg ggcatcacat cagggagtaa
    10021 tgtattaacg cttgtacaac ggaccgtagt tctggcaagc gcggtaatcc tggccttcaa
    10081 tatccatgcc gtgcgcagcc caggcagaag gacgatacac tttggtctct tcaacgttgt
    10141 gcatacatac cgggatacgc agcatggagg caagagtgat aaagtctgcg ccaacgtggc
    10201 cgatggtcag aactccgtgg ttggcacccc agttcgccat taccgaatac acatccgtaa
    10261 acggcccttt accagtgagg cgcggtgcaa accaggtggt tggctaggtt gagttggtgc
    10321 gtttgttgag gatgtcatgc acatccttcg gcaattccac gctccagcct tccgcgattt
    10381 gcagtaccgg tcccaggcct ttgatgatgt tgacacgagt catggtgaac gggacgccgc
    10441 cttcggtaag gaagcgggaa gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc
    10501 accattcggt agcggcgagg caagcgtcag cctcttgctg agagatttcc cagtgcggct
    10561 tcatcgtcgg gttaccttcg ctgtcgcgtt gtttacagga accgtccagc gcagcagaac
    10621 ctgagttgat caaatggatg atgccgtgtt ctgccagtcc gtccagttta tgccccgtta
    10681 cacgctcaat tgcttctggt gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc
    10741 cggtgagctg gtgacccatt agcattgcca cgccgttaag actgtcgttt tcggtcgcca
    10801 cgacaaaggg ttcgcgcacg ccattccagt caaatgaact gttgaggatc gcttcggcgg
    10861 tgtcaccatt gggatattga tcggtccagt gacgttgccc ctggaagccc gcagcgatgg
    10921 cgttgtagcc aagtgattct tccacgcgac caatatcggc cagtttgctg ttgccttgca
    10981 tcatgtcgcg gatacacatc gccatcagta aactttcgcg cagaactgcg cggctttgct
    11041 cggcattgcg ttgatactgt ttgttatttt catcttcgcc atagcggaag tttttatcag
    11101 cccaggccag tgccatttcc agttcggctt cgtcgtaaat cttctgatcg atacggcgac
    11161 gcagttcggt catatccacc gcctggactt tcattcccag ccaggattca aagaagttgt
    11221 gatcaacaat agaaccggcg atacccatcg aaacgccgcc cagcgacaga tagcttttac
    11281 ctttcatgct ggcgacggcc aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat
    11341 cggcaggaat cgatgtgtca tcggcatcct gaacgtcatg accgtaaatg gagaatgccg
    11401 ggatgccttt ctggctgtga gctgccagag ccgctgccag gtaaacagcg ccggggcgtt
    11461 cagtgccgtt aaagccccaa atggctttcg ggcgggttgg atccatgtcg atggtttcac
    11521 tgccatagca ccagcaaggc gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt
    11581 tttcttcgca agcagcggct tcagccatac ccgcgataca ggtatcggaa atgacacact
    11641 cgacggcagc tccgcaggca tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg
    11701 ccatattcat tgtttgttct tcaagcgact cacgaacacc catgcgacga ccgtcaataa
    11761 ccgggcggat accaattttc ggtaagctga tttttttcat tacagattcc tcactttatt
    11821 cggaaaatat gttcagttag ttgccgtttg agaacggaaa cgggcaaaga taaagatgac
    11881 cgcgaagcag agtgcgggga tcagttcagc agtggggatg ttgcccgccg cgtcactgac
    11941 aaaacccatg accggagtga caataccgcc gccaataatg gtcataacga tgaaggacga
    12001 accgtatttg gtgtcctggc cgagattctt aatgcccagc gagaagattg ttgggtactg
    12061 aatcgacata aaggcgctgc ataaagtcag ggctattaag cccacatgac cgccagcgaa
    12121 ggctgagatc aggcacagtg ccatagcgat taatgcgtag gcggccagga ctttgtgtgg
    12181 tgcgaagcga ctgatgagcc aggtaccggt gaaacgacca ataaagaagc acaccatggt
    12241 tccggttaaa tagttagcgg caaagcctgc agtcatacct ggaatttctt ctacagcgta
    12301 gcgaatcaaa tagctccagc aggccgtttg tgcgccgaca tagcagaatt gcgctaatac
    12361 cgcccagcgc cagtgacgaa tacgcgccag acgagaaagc gatgcggaga acgatccttg
    12421 tttggcgtca ctgtgattat cactctgcaa tgccgggaat ttcgtcagca tgatcagcag
    12481 ggcgaccagt aacacgatag ccacgatgat catataaggt gtctgtaccg ataataccag
    12541 gctgtgttta tacgcactca attgctctgg agacatttta tcgagaacgt cttgcgattg
    12601 atgtggcacg ttagacaaaa taagactttg cccaaagaca accgcgataa ttgcgccaaa
    12661 cgagttaaat gtttgcgcaa gatttaagcg gaagtgacta ctgctttccg gccctaatac
    12721 cgtaacaaaa gggtttgcgg cagtttccag acaacctaat ccggctgcaa taataaatag
    12781 gccaactaaa aataaggtgt agttcattat ttctgcagcg ggccagaata atgcagcacc
    12841 caaggcatat aaaaataatc cggtaataat ccctgcttta taactgagtt ttttcatcaa
    12901 tatcccagca gggattggga taatgaaata accaaagtaa aaggccgatt ggatcaggcc
    12961 agcctggaaa tttgtcagcg taaaagcctg ctggaattga ggtaataaaa tgtcgttaag
    13021 gttattggct accgcccaaa gaaaaaacag tgagcacagc agcgcgaatg gaataatgta
    13081 acttctgctt tgccctgcat ctttatctac cgcacggtaa ctctgcgttt gtattgatgt
    13141 gtttcccata gcatcctctt aggttcagaa gcttaagcac gtaatcggtt atgttttcaa
    13201 aagtgcaaaa ccagcgagtt cgggcttcat atcggtgaat atttgcctgt ctgaaatgaa
    13261 ctcatggatt cattttcaaa taaaaaacgc tctgtaatat gacggcggtc acacttatta
    13321 gctggaaggt gactttatgt gactaccatc actttaataa tcgaaatttc atgtccgttt
    13381 cagaaaataa atcataaaaa acacaaacca gatgaccgta ttaattgttt gtgccgaaaa
    13441 cgggcatttc tggcgagggg aaatggaaaa atgaccgttt tgataaatat ttttaagagc
    13501 ggtcattttt aacggggtta taaaaagaga acttgcagtt atttactgtg attagttgag
    13561 ctaggtcaca aaatataagc ttgttaattt ctttcttgtt aatcaggaag aagatggtgg
    13621 gtaattaaac ggctaattca atagtgtgaa aggaacaaca ttattgccct gttttgaatc
    13681 agagagaggt agctaatgga acgaaataaa cttgctcgtc agattattga cacttgcctg
    13741 gaaatgaccc gcctgggact gaaccagggg acagcgggga acgtcagtgt acgttatcag
    13801 gatgggatgc tgattacgcc tacaggcatt ccatatgaaa aactgacgga gtcgcatatt
    13861 gtctttattg atggcaacgg taaacatgag gaaggaaagc tcccctcaag cgaatggcgt
    13921 ttccatatgg cagcctatca aagcagaccg gatgccaacg cggttgttca caatcatgcc
    13981 gttcattgca cggcagtttc cattcttaac cgatcgatcc ccgctattca ctacatgatt
    14041 gcggcggctg gcggtaattc tattccttgc gcgccttatg cgacctttgg aacacgcgaa
    14101 ctttctgaac atgttgcgct ggctctcaaa aatcgtaagg caactttgtt acaacatcat
    14161 gggcttatcg cttgtgaggt gaatctggaa aaagcgttat ggctggcgca tgaagttgaa
    14221 gtgctggcgc aactttacct gacgaccctg gcgattacgg acccggtgcc agtgctgagc
    14281 gatgaagaga ttgccgtagt gctggagaaa ttcaaaacct atgggttacg gattgaagag
    14341 taatttcgta aagcaacaag gagaaggatg atggctaaca gaatgattct gaacgaaacg
    14401 gcatggtttg gtcggggtgc tgttggggct ttaaccgatg aggtgaaacg ccgtggttat
    14461 cagaaggcgc tgatcgtcac cgataaaacg ctggtgcaat gcggcgtggt ggcgaaagtg
    14521 accgataaga tggatgctgc agggctggca tgggcgattt acgacggcgt agtgcccaac
    14581 ccaacaatta ctgtcgtcaa agaagggctc ggtgtattcc agaatagcgg cgcggattac
    14641 ctgatcgcta ttggtggtgg ttctccacag gatacttgta aagcaattgg cattatcagc
    14701 aacaacccgg agtttgccga tgtgcgtagc ctggaagggc tttccccgac caataaaccc
    14761 agtgtaccga ttctggcaat ccccaccaca gcaggtactg cggcagaagt gaccattaac
    14821 tacgtgatca ctgacgaaga aaaacggcgc aagtttgttt gcgttgatcc gcatgatatc
    14881 ccgcaggtgg cgtttattga tgctgacatg atggatggta tgcctccggc gctgaaagcc
    14941 gcgacgggtg tcgatgcgct cactcatgct attgaggggt atattacccg tggcgcgtgg
    15001 gcactaaccg atgcactgca cattaaagcg attgaaatca ttgctggggc gctgcgagga
    15061 tcggttgctg gtgataagga tgccggagaa gaaatggcgc tcgggcagta tgttgcgggt
    15121 atgggcttct cgaatgttgg gttagggttg gtgcatggta tggcgcatcc actgggcgcg
    15181 ttttataaca ctccacacgg tgttgcgaac gccatcctgt taccgcatgt catgcgctat
    15241 aacgctgact ttaccggtga gaagtaccgc gatatcgcgc gcgttatggg cgtgaaagtg
    15301 gaaggtatga gcctggaagg ggcgcgtaat gccgctgttg aagcggtgtt tgccctcaac
    15361 cgtgatgtcg gtattccgcc acatttgcgt gatgttggtg tacgcaagga agacatttcg
    15421 gcactggctc aggcggcact ggatgatgtt tgtaccggtg gcaacccgcg tgaagcaacg
    15481 cttgaggata ttgtagagct ttaccatacc gcctggtaaa tgcgctgatg tgataatgcc
    15541 ggatacgatt tgcggcgtat ccggctcgcc gttaccgtac caaccgcaat tgctgtaaat
    15601 tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
    15661 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
    15721 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
    15781 caacacccgg tacctttgaa ctgctgatcc ccgcaagtcc ccagtaatcg ggcaactgct
    15841 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
    15901 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
    15961 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
    16021 cttcgttgcc gctggctgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
    16081 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg atccgctttg taatctggta
    16141 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg accgcgtggg
    16201 ttggctggct gtgcataatg agctgatcga gcgcatgctg gcaggtttcg acacagggcg
    16261 aaccctgaac ggcatgaatg cgacggataa gattcagtgc atcgacaata agcaaatgaa
    16321 cagccacgat aaccccctta aagatcaggc gataagggta acattcgatg ccggaacagg
    16381 ctatggctgg ttgagaaaac aggaagaggc ctcgcaaaac gaggcctctg ggagagatta
    16441 atcgcaggca acgatcttca tcgccaggcc gccgcgagag gtttcgcggt acttggcgtt
    16501 catgtcttta cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggttc
    16561 gctggtacgg cgcagtgcca tacgcgcggc gttcaccgct ttcactgctg caatggcgtt
    16621 acgctcaatg cacggtacct gaacctgccc cgcgaccggg tcacaagtca gaccgaggtt
    16681 gtgctccatc gcgatttccg ctgcgatgca aacctgcgcc gggcttgcgc ctaacagttc
    16741 tgccagacca gctgccgcca tagagcacgc cacgccaact tcaccctggc aacccacttc
    16801 ggcaccagaa atcgacgcgt tcatcttata aagagaacca atggcgctgg ctaccagcag
    16861 gtaacgagcc agtgagttag cgttcacttc gcggataaac ttatcgtagt acgccagcac
    16921 agccgggata atcccgcacg caccgttagt cggcgcagtc accacgcgac cgccagcagc
    16981 gttctcttcg ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc
    17041 agtggtggtt ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac
    17101 gcgcagtttg ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat
    17161 gacttcccag acgttcgcca ggtgctgttc cagctcttct ttactgtgca gcgccagctc
    17221 gtttttcatc atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc
    17281 ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg ctatcctgct ggccaaaatg
    17341 ctcttcatca acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt
    17401 atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg
    17461 gaagttcatg cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag
    17521 gcgaccatga gtattcacat cctgaataaa accggggatg gaatcgatat ccacggtatc
    17581 cggcaggtta cccgctaggc ccataataat ggcgatatct gtgtggtggc ctttaccagt
    17641 cagagagagc gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcaggttacg
    17701 ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact
    17761 ggaagggcca atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg
    17821 ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta
    17881 gaagattgcg gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc
    17941 gctgtactta cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat
    18001 catcgcgatg attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt
    18061 ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat
    18121 ttcgatagac ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg
    18181 tgcgcccagg tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc
    18241 catccacgcg ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt
    18301 ctgctcttta gccgcagcca ggtctgccgg agtcaggctc agtacgcagc tgaagacgaa
    18361 gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg
    18421 ttctgccata tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga
    18481 gtggttgaac gagaacacca ttaccggaat tgccagccac agggtcatcc acagaccgtt
    18541 tccggttgca gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg
    18601 gatcaggtac agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat
    18661 cgctttaacg atcatctgct caccaaagcg aacgatggtc atcataccca cgatcaggat
    18721 cagcgacaga atcgcacgcg gcggtggcgt catacccagc tggtgagaca tgaagctttc
    18781 aacggtattg gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta
    18841 gagcagggta atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat
    18901 gtcttcgccc gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa
    18961 cgtcatcggg aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc
    19021 gttgattggc aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca
    19081 catggtgtct gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg
    19141 agtcgtttcc atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag
    19201 cccgggaagg tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt
    19261 accgccgtaa taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct
    19321 tgatcccaaa tacaataatc tacttggaag atagattatc ccaacgcttt actctgtaaa
    19381 tcaaaagtaa aagagaattt aagggcaata tttgcccatt aaacgacatc tgacttcttc
    19441 cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc
    19501 gataatttct ttttcatagc gataaaatac ggtttatgaa aatagggaaa cgtttgcgtt
    19561 gcaacataaa ttgtatgaca tattaactgt gatgatattt gtcgcgtgag ggtggtaaga
    19621 aacattcata taacaaatga aatgagttga tgagtaagtg accaaaaacg agaaaacccg
    19681 gcaacaaacg tcaccgg
//
LOCUS       DADLSE010000073        19368 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLSE010000073 DADLSE010000000
VERSION     DADLSE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.073"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JN692_002494"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="JN692_002494"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7264779.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JN692_002495"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="JN692_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7264780.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JN692_002496"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="JN692_002496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7264781.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JN692_002497"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="JN692_002497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7264782.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JN692_002498"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="JN692_002498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7264783.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JN692_002499"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="JN692_002499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7264784.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JN692_002500"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="JN692_002500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7264785.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JN692_002501"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="JN692_002501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7264786.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JN692_002502"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="JN692_002502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7264787.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JN692_002503"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="JN692_002503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7264788.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JN692_002504"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="JN692_002504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7264789.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JN692_002505"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="JN692_002505"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7264790.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JN692_002506"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="JN692_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7264791.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JN692_002507"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="JN692_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7264792.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JN692_002508"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="JN692_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7264793.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="JN692_002509"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="JN692_002509"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7264794.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JN692_002510"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="JN692_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HAY7264795.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVLERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JN692_002511"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="JN692_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7264796.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELQEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4619 a         5126 c         4857 g         4766 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg cgccttccag
      421 cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttatcctg agacagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcatcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccattatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcgag tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg gctttcgtgc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc aatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctggggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gttcaacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaagaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgtt gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaagcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 agtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tggccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 tgcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacgctaa agtcgatggt
    13201 tcgagcatca atacgcggcg gagtaccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagagagcg gaatggatgg cggatcgcca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt tgccgtcgag gaacgtccca acggtgagca ccacagcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagcgca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcagccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg acttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa cagcggttgt tctttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actgccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aaccagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gctgatcagg gcttgatggt acgcatgatc
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca taacttttat aaagatgctg
    14881 tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 ttcttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac cgttcctgcc ggatcagatt
    15841 cacttcgtac acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt
    15901 gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagttg
    15961 agtgatggtc atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggcc atgcgggcct gaacggcgat attctggtgt ggaacccggt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata acagcgccgg ttttcagcga
    16741 gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg agcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgct aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctgacc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 aacgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gccgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg
    18241 acgacggctg aaaatgccgc ccagacgaat taacgttaag gctgttttat cgctttgttg
    18301 ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg cgggtcaaca tcccttgctg
    18361 ttgccaggca tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatca accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa gcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cttgcacttt atctaaccat agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc ctgcagctcg ttggaggccg ccaccagtag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacgatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DADLSE010000074        19219 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLSE010000074 DADLSE010000000
VERSION     DADLSE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.074"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            8..2287
                     /locus_tag="JN692_002512"
     CDS             8..2287
                     /locus_tag="JN692_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7264797.1"
                     /translation="MLVETDGETVFSSRGALATGMENSLQSAVRDQVHSNTRVRFPMV
                     RKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIREAYGPASIFAGSYGWRSN
                     GVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVVGGSEVYQQQTSWPLVLEH
                     SDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKLICIDPMRSETVDFFGDKM
                     EWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYAVFASYLLGESDGIAKNAE
                     WAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGEQKHWMIVTLAAMLGQIGT
                     PGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDKIPVARIVEALENPGGAYQ
                     HNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISECFWTAAAKHADIV
                     LPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAELSERWEKGGYARF
                     TEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPENPDSERFIRFADF
                     CRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGNAEPEQLQVLSAH
                     PAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRVWNSRGQILAGAV
                     ISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLGNGCAGNTALAWL
                     EKYNGPELTLTAFEPPASS"
     gene            complement(2256..2696)
                     /locus_tag="JN692_002513"
     CDS             complement(2256..2696)
                     /locus_tag="JN692_002513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7264798.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN692_002514"
     CDS             complement(2693..3256)
                     /gene="tag"
                     /locus_tag="JN692_002514"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7264799.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKAPKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3414..4112
                     /locus_tag="JN692_002515"
     CDS             3414..4112
                     /locus_tag="JN692_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7264800.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4341..5549
                     /locus_tag="JN692_002516"
     CDS             4341..5549
                     /locus_tag="JN692_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264801.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5873..7564
                     /gene="eptB"
                     /locus_tag="JN692_002517"
     CDS             5873..7564
                     /gene="eptB"
                     /locus_tag="JN692_002517"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7264802.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDNYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7656..7732
                     /locus_tag="JN692_002518"
     tRNA            7656..7732
                     /locus_tag="JN692_002518"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7690..7692,aa:Pro,seq:cgg)
     gene            8476..10083
                     /gene="dppA"
                     /locus_tag="JN692_002519"
     CDS             8476..10083
                     /gene="dppA"
                     /locus_tag="JN692_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7264803.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVSPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10391..11410
                     /gene="dppB"
                     /locus_tag="JN692_002520"
     CDS             10391..11410
                     /gene="dppB"
                     /locus_tag="JN692_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7264804.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11420..12322
                     /gene="dppC"
                     /locus_tag="JN692_002521"
     CDS             11420..12322
                     /gene="dppC"
                     /locus_tag="JN692_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7264805.1"
                     /translation="MSQVTENKVISAPVPMAPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12333..13316
                     /gene="dppD"
                     /locus_tag="JN692_002522"
     CDS             12333..13316
                     /gene="dppD"
                     /locus_tag="JN692_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7264806.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13313..14317
                     /gene="dppF"
                     /locus_tag="JN692_002523"
     CDS             13313..14317
                     /gene="dppF"
                     /locus_tag="JN692_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7264807.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14347..15618)
                     /locus_tag="JN692_002524"
     CDS             complement(14347..15618)
                     /locus_tag="JN692_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7264808.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16094..16201
                     /locus_tag="JN692_002525"
     CDS             16094..16201
                     /locus_tag="JN692_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY7264809.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN692_002526"
     CDS             complement(16288..17967)
                     /gene="bcsG"
                     /locus_tag="JN692_002526"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7264810.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN692_002527"
     CDS             complement(17964..18155)
                     /gene="bcsF"
                     /locus_tag="JN692_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7264811.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN692_002528"
     CDS             complement(18152..>19219)
                     /gene="bcsE"
                     /locus_tag="JN692_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7264812.1"
                     /translation="FGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLV
                     QSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSL
                     QQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPL
                     SRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLP
                     AHGKGVLVALRPVPGIRVEQAQTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALN
                     HIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDG
                     RHWRRIPEPMRLLDDAVERSS"
BASE COUNT         4585 a         4818 c         5163 g         4653 t
ORIGIN      
        1 ggggcccatg ctggttgaaa ccgacggcga aaccgtgttt agctcgcgtg gcgcgttagc
       61 cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac caggttcaca gcaatacgcg
      121 ggtacgattt ccaatggtgc gcaaaggctt tctcgcgtca ccggaaaacc cgcaaggcat
      181 tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag gcgctggatc ttattcacca
      241 acaacataaa cgcattcgtg aggcttatgg tccggcatcg atttttgctg gttcctacgg
      301 ctggcgttca aacggcgtgc tgcataaggc ctcgacatta ttacaacgct atatggcgct
      361 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
      421 gccgtatgtc gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
      481 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
      541 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa
      601 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
      661 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
      721 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
      781 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc
      841 ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
      901 aaataccacc atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa
      961 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
     1021 ttttggtctt tcttaccatt ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct
     1081 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc
     1141 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     1201 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     1261 ggataccaat cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg
     1321 cttctggacg gcggcggcga aacacgcgga tatcgttctg cctgcgacta cctcttttga
     1381 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
     1441 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga
     1501 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct
     1561 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc
     1621 atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga
     1681 gcggtttatt cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc
     1741 cagcggcaag attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc
     1801 tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt
     1861 gcaggtactt tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct
     1921 gcgcgaattg tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca
     1981 ggcgcgcggc ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct
     2041 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
     2101 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
     2161 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cggcgctggc
     2221 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     2281 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     2341 ccctgcgcac ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     2401 tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc
     2461 ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     2521 agcttaccgt cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     2581 agcggaatgc agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     2641 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     2701 tatttcccgg atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     2761 taaaggagta acagattgtg gtgccgacaa acttaaaacc acgttttttc ggtgccttag
     2821 atagggcgtc ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca
     2881 cctgtggctg atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt
     2941 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga
     3001 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca
     3061 ccggatcgaa ctgatgaaag caggcgcgat agttttcgcg ttttttgagg acggtgatcc
     3121 acgataaccc agcctgctgc ccttcaaggc agatcatttc gaacagtttt ttactgtcag
     3181 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggtcc tgactcaccc
     3241 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     3301 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     3361 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     3421 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct
     3481 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     3541 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     3601 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     3661 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     3721 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     3781 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     3841 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     3901 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     3961 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     4021 atattgccgc ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     4081 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     4141 taacaacagt tgtggcgata gtgggtgcta acttatcaaa taataaattt ggtgaataat
     4201 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
     4261 gtttttcccc aggcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt
     4321 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
     4381 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacctgg agcctgttta
     4441 atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     4501 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
     4561 gcgttaaacg cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg
     4621 cgcattccaa caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     4681 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag
     4741 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     4801 tcgacacgca gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga
     4861 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     4921 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     4981 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     5041 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
     5101 atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt
     5161 ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
     5221 tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg
     5281 tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg
     5341 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
     5401 tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt
     5461 tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa
     5521 tgttgcgtga ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt
     5581 ccgtgtgcat caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag
     5641 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
     5701 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
     5761 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
     5821 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
     5881 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
     5941 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
     6001 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
     6061 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
     6121 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
     6181 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
     6241 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
     6301 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt
     6361 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
     6421 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
     6481 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
     6541 atcttccgat aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca
     6601 aaacgttgat gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
     6661 gggtattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
     6721 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
     6781 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
     6841 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
     6901 ctacagcaac acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg
     6961 taatcgtggc aagccggtag atgatatgtt gctggtagat gaaatgcagc aatcgctagg
     7021 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
     7081 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
     7141 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
     7201 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
     7261 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
     7321 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataact atctggaaaa
     7381 tccggccaat gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
     7441 ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
     7501 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
     7561 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
     7621 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
     7681 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
     7741 aagcctgctc aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg
     7801 gggcagggag gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
     7861 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
     7921 ttttcctgcc aactttgtgc cccatatctc actatccatt aacggatttg tgacagaaac
     7981 cattgtcttt ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca
     8041 ttttccgctg tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc
     8101 agattttttt cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga
     8161 ttgttctggt tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt
     8221 ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg
     8281 ttttcacatt ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact
     8341 gttacactga tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa
     8401 aaaacaaaaa aggtcaggca gcgacaaccc attgcaaagg gttaaaacaa caaacatcac
     8461 aattggagca gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc
     8521 agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca
     8581 gaaggatctc cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc
     8641 tcttccgtcc cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgagc
     8701 ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg
     8761 cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc
     8821 gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt
     8881 tctggcggca gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg
     8941 aaaaaggtgg acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc
     9001 gctgacctgg caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg
     9061 aaagccggta caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta
     9121 cagcagtacc aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc
     9181 aaaccgcaga tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg
     9241 aaattgcaga agaatgaatg ccaggtgatg ccgtacccga acccggcaga catcgctcgc
     9301 atgaagcagg ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc
     9361 tcgtataacg tgcagaaaaa accgctggat gacgtgaaag ttcgccaggc actgacctac
     9421 gcggtgaaca aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa
     9481 aacctgatcc cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat
     9541 cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac
     9601 ctgtgggcga tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg
     9661 attcaggcag actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt
     9721 gagtacctca agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc
     9781 gataacgggg acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa
     9841 ggttctaact actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt
     9901 gctaccgacg accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac
     9961 gatcaggctc cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa
    10021 gtcaaaggct atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa
    10081 taattaaaag ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat
    10141 caggcctacg aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc
    10201 gttaacgccg catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg
    10261 cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg
    10321 gcgggaattg atttgtgagc aatacagaca cgcagttcca ggctgcgggt cactatagag
    10381 aatccgggtt atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat
    10441 cggtattacc cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat
    10501 catggcaggc gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg
    10561 cttagataaa ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga
    10621 tctaggcatt tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca
    10681 ggccacgctg gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt
    10741 tggtgtgctg gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcttggc
    10801 gctgaccggc tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc
    10861 ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga
    10921 ctccaatccg ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa
    10981 ctttatcgat gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct
    11041 ggcggtcatt gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat
    11101 ccgcaccgcg cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg
    11161 taacgcgatg ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg
    11221 ggcgattctg accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc
    11281 actgcaacgc cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat
    11341 tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca
    11401 taagaagtaa ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt
    11461 gccgatggcc ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt
    11521 cgggctggtt tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc
    11581 ttataacccg gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg
    11641 cggcagtatg gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct
    11701 gatgtacggc gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat
    11761 tatgggcgtt attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat
    11821 catgcgcgtg gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt
    11881 ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc
    11941 gcactatgtg cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac
    12001 cgcctctcgc gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa
    12061 ctgccttgcg ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat
    12121 ggctgctctt ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat
    12181 gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct
    12241 ggcgatcctg ctgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct
    12301 cgatcccaaa ctgaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat
    12361 cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg
    12421 taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt
    12481 cactggcgat tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt
    12541 ttaacggcca ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg
    12601 aagtggcgat gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt
    12661 tccagattat ggaagcgatt aaggtgcatc agggcggcaa caaaagtacc cgccgtcagc
    12721 gggcgattga cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt
    12781 acccgcatca gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct
    12841 gtcggccaaa actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg
    12901 cgcaaatcat cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa
    12961 ttacccatga cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag
    13021 gtcaggtagt agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata
    13081 ctcaggcatt gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt
    13141 tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct
    13201 gcccctatgc cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg
    13261 ggcgtcagtc caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac
    13321 gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc
    13381 ggtgaagaaa ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt
    13441 taaccttgaa cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac
    13501 cctcggtcgg ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg
    13561 gcaggatctg ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat
    13621 cgtcttccag aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga
    13681 agagccgctg cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc
    13741 gatgatggca aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc
    13801 cggcggacaa cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt
    13861 gattgccgat gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct
    13921 gatgatggat ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc
    13981 ggttgtggag cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa
    14041 aggaacgaag gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc
    14101 cgcgacgcca cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact
    14161 gccaagcccg ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt
    14221 cggcccctgc acccagttgc agccgcagct aaaagactac ggcggtcaac tggtagcttg
    14281 ttttgctgtt gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc
    14341 tccggtttac tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag
    14401 aatcagccag gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa
    14461 aaacggaatg aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag
    14521 cactgataca cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc
    14581 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
    14641 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
    14701 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc
    14761 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
    14821 tgagaaggta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
    14881 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
    14941 ggcaatattc attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat
    15001 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa
    15061 attccagtgc gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac
    15121 cattggccca gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac
    15181 ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
    15241 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
    15301 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
    15361 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
    15421 gtcctgcact acccaggtgg caggataagc aatgatcgct gcggtaataa atacccaaat
    15481 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
    15541 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga
    15601 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
    15661 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag
    15721 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
    15781 taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct
    15841 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc aatcgtagaa
    15901 tcagcggcaa gccggaaagg ttccggtgag gcgcaatgtt gcgggggctt tatccctggt
    15961 ggcattggtt gctggagaga gaaaaccccc gcacgttgca ggtatgcacc tgacaacatc
    16021 acgggggcta atcttgaacc caaacaactc aagaatagcc gcgaaacgtt ggcattacaa
    16081 cacaggcggc tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc
    16141 tccggtcatt gctggcattc ttgccagtat gatcgtgaac tggctgaata agcggaagta
    16201 acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcaga
    16261 ggttaaactc tgcggccttt ttcgtgctta ctgcgggtaa ggcacccagt cgccgccgtt
    16321 caggcgaacg tacggtttat cttggtattg aataactact gcatttgagt tctcggagac
    16381 cggtgctgtt tgtggcaacc cactggtgag ttttttccag tcaacattgt cttcggtgaa
    16441 aatcttgcca tcaagaacgc gaaccaccag atcggagata gccaggaagc tgctcggttg
    16501 gtcaatgaca atcggtgccc cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac
    16561 ggggacgtct gtgatagacg ggctagggat atcacgtagg ccagatacct gcattctgtc
    16621 gcccttcagc gcgccgccgt gttccggcac cacgaccacc atcactttac gacccgattt
    16681 ctccagttca gtaaagaagg cgtccagttc atcaaagaat ttctgcgccc gcgctttgta
    16741 atccgctgtt ttgctgaccc ccggataatg gttgccgtca tgcagtggaa gcgtgttgta
    16801 gaacgtggca ctacggctgt ttttatcttt ttcggtaacg tccagccagc ggttaagcac
    16861 ggcggtatcg tcataaaccg gcgaaccatc aaagcccagc aaaataaccg gcagatttgt
    16921 ttgatccatc aattcagtct gcatgccgcc attttcgcga acttctttca aaaaaccgcc
    16981 gaactggccg ttatgtccca tcatcaggtg ctgggtaaag cccagtttcg aaaggttatc
    17041 aaacagatag cagtcgttat ttgccggttg atacagatta gtgtgcgaag tctgcccgca
    17101 gctggcgcgc agtaaacgga tcgccgccgg gccactgtag gaggtggcgg agttaaagtt
    17161 cttgaactca atatcgaaat gcgaccacag tggatgcgac atcaacccgg cggcttctat
    17221 atccgaccag gaaagcgaac agatgttaat caccagtagt tcaaatggct gagcatcagc
    17281 gggcagcgaa gacgggaagg tcgatttacg tttcgcctcc gcgttataga aattattcag
    17341 ccaggcgtta aggttcgccg ttgttggcgg tgcagtttgt gcgggcatat cacccactac
    17401 cggtgcgcca cccgtcgccg caacggttgc cgctgcgtta ccacccgtcg ttgttacagt
    17461 ggtcgtcggt tgtccggctg gccacaagga gaaacttggt cccgccaggg taagtacgtt
    17521 cagccatagc agtatggcaa ccacaaaaac ggtaatgcga atccattgtg acaggaataa
    17581 ccaggccact aataaaacaa aaatggcccc aatcatctgc cagttaataa agcgtgtgac
    17641 aaggtcgatt aaataatcgg tactgaaccc cgccacctgc gaaccctggc tcattatgct
    17701 ttccgggcca ggcaaccagg tgtcatgcca gaacaaagca aagccgatcg gcagggcaat
    17761 ccagtggcgc aagcgatgca ggctgtaacg cggaatgggc atcagcagaa acgcggcaaa
    17821 caccaaattg aggagcgggt ggaagttaag atatcccgcc cacaacaggc caaacttaac
    17881 cagaaaatag aagttccagc cggaaaggcc gcgccagtat tgccagaggg aagaaggcat
    17941 ggcggtattt tgcgtaaatt gagtcatttt ttggttgccc tggctttttc cgtgcggcgt
    18001 aacgtcccgg ccggtttaac ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga
    18061 atgcgtcgca aagagtgccg cgccagatag cccagcggga aaaatatcag tgcgcaaacg
    18121 acaataattt caatgatatc gctgatggtc atcatgatga gcgttccaca gcatcatcta
    18181 acagtcgcat gggttctggt attcgtcgcc agtggcgacc atcgtgctcg gcattgatga
    18241 ccggtttaga actttgcgtt aaaggcagcg gcattcccca ttgttctggg gcaagcaggc
    18301 gcatctgcac cagctcggca ctgatttgat catcttcaaa ccagaccata cggtttgaga
    18361 aaatgtcgcc agtaggcaat gggaaaatat gattcaacgc ggtatccaga tcgttaatcc
    18421 gacagaatga gagaaacagc accagccgat taccgccaat ggtcatgata tcgccggtac
    18481 ggttagggcg acatagcgtc tgggcttgct caacgcggat acccggtacc ggacgtaggg
    18541 caaccagaac gcctttaccg tgggcaggca agagagggtt attcatcatg ttgttgacgg
    18601 cattacagaa cacatcccac ttctggaaac cacgcagttt gagcggctgg gtcattgaca
    18661 gcaaggtagt gatatcttcc ggtacatagc gactaaactt ctgcccttgc acgctttcga
    18721 tcatcgtcag acaacgggag agtggcgcat tccacggaat aaccatattt gcaccgcagg
    18781 ccaataacaa acgttcatcg gtggcgcgca ggctagcggt attttcccgc acgaggattt
    18841 tcatcgcact accgcgctgg cgacgcaggg tatgaatgct gcgggccagt ggctcgattt
    18901 gcgcattttg ctgtaaagaa aagaccaccg tcgccgcctg agcggtacgg gcttcattga
    18961 acaggacttc gttattgttg aacagttgcc agtgttccga tagcggcggc gcaccttcca
    19021 gtacagcaac attactcaga atgcgttttt cgtcgctgcg tggttggatc tccgcctctt
    19081 cgctttgaac taatgtccag ataccatttt gttgctgaac gctaagctgc tgacgggcgc
    19141 tgaccccttt ttcgttgcac cagaaggcaa tatccagcaa atgttggtca ccctgaaaac
    19201 gcaaactggc aagaccaaa
//
LOCUS       DADLSE010000075        18940 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLSE010000075 DADLSE010000000
VERSION     DADLSE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.075"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            428..1009
                     /locus_tag="JN692_002529"
     CDS             428..1009
                     /locus_tag="JN692_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7264813.1"
                     /translation="MKQQEEHNNKIVLLEKQQAQLKSQLETIQKQQTGIINSTKNLTH
                     VIKSVKDQQNTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLL
                     STPISNIGFNATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVS
                     PNNLGYLKLAINMENIQFDNLPSAENRQKRSKK"
     gene            1053..5715
                     /locus_tag="JN692_002530"
                     /pseudo
     CDS             1053..5715
                     /locus_tag="JN692_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            6083..7738
                     /gene="lldP"
                     /locus_tag="JN692_002531"
     CDS             6083..7738
                     /gene="lldP"
                     /locus_tag="JN692_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7264814.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7738..8514
                     /gene="lldR"
                     /locus_tag="JN692_002532"
     CDS             7738..8514
                     /gene="lldR"
                     /locus_tag="JN692_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7264815.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8511..9701
                     /gene="lldD"
                     /locus_tag="JN692_002533"
     CDS             8511..9701
                     /gene="lldD"
                     /locus_tag="JN692_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7264816.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9897..10370
                     /gene="trmL"
                     /locus_tag="JN692_002534"
     CDS             9897..10370
                     /gene="trmL"
                     /locus_tag="JN692_002534"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7264817.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10423..11244)
                     /gene="cysE"
                     /locus_tag="JN692_002535"
     CDS             complement(10423..11244)
                     /gene="cysE"
                     /locus_tag="JN692_002535"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7264818.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAISIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11324..12343)
                     /gene="gpsA"
                     /locus_tag="JN692_002536"
     CDS             complement(11324..12343)
                     /gene="gpsA"
                     /locus_tag="JN692_002536"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7264819.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12343..12810)
                     /gene="secB"
                     /locus_tag="JN692_002537"
     CDS             complement(12343..12810)
                     /gene="secB"
                     /locus_tag="JN692_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7264820.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12873..13124)
                     /gene="grxC"
                     /locus_tag="JN692_002538"
     CDS             complement(12873..13124)
                     /gene="grxC"
                     /locus_tag="JN692_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7264821.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13266..13697)
                     /locus_tag="JN692_002539"
     CDS             complement(13266..13697)
                     /locus_tag="JN692_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7264822.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13942..15486
                     /gene="gpmM"
                     /locus_tag="JN692_002540"
                     /gene_synonym="pgmI"
     CDS             13942..15486
                     /gene="gpmM"
                     /locus_tag="JN692_002540"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7264823.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15496..16779
                     /gene="envC"
                     /locus_tag="JN692_002541"
     CDS             15496..16779
                     /gene="envC"
                     /locus_tag="JN692_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7264824.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16783..17742
                     /locus_tag="JN692_002542"
     CDS             16783..17742
                     /locus_tag="JN692_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7264825.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17729..18763)
                     /gene="waaH"
                     /locus_tag="JN692_002543"
     CDS             complement(17729..18763)
                     /gene="waaH"
                     /locus_tag="JN692_002543"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7264826.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPIDRLRSTGVLTGPDWLRMGLFSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
BASE COUNT         4796 a         4867 c         4874 g         4403 t
ORIGIN      
        1 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
       61 attatctgta aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      121 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      181 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      241 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgatcgct
      301 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      361 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      421 tagtttactg aaacaacaag aagaacataa caataaaatt gtgctacttg aaaaacaaca
      481 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      541 tacaaagaat ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      601 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      661 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      721 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      781 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      841 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
      901 tgtatcgccc aataacctag gatatctgaa gttagccatc aacatggaaa atattcagtt
      961 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1021 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1081 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1141 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1201 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1261 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1321 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1381 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1441 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1501 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1561 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1621 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1681 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1741 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1801 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1861 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     1921 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     1981 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2041 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2101 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2161 tagcaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac
     2221 caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg
     2281 atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta
     2341 gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta
     2401 aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa gccaacacca
     2461 ccaatatcac caacctgacg gattccgttg gtgaccttca ggctgatgcc ctgctctgga
     2521 acgaaactaa aaaggcattc agtgcagctc acgaccagga taccaccagc aaaatcacca
     2581 acgttaaaga tgccgacctg acggctgaca gcactgatgc tgttaacggc tctcagctga
     2641 aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat cgccaataac acttccaata
     2701 ttgccactaa caccaccaac atctctaacc tgactgagac ggtgactaat cttggtgagg
     2761 atgcgctgaa atgggataag gacaatggtg tattcacggc agctcatggc accgagacca
     2821 ccagcaaaat aaccaacgtt aaagatggcg acctgacgac tggcagcacc gatgccgtta
     2881 acggctctca gctgaaaacc accaacgatg ccgtggcgac gaataccacc aatatcgcca
     2941 ctaacaccac caacatctct aatctgactg agacggtgac taatcttggt gaggatgcgc
     3001 tgaaatggga taaggacaat ggtgtcttca ctgcagctca tggcaacaat accgccagca
     3061 aaatcaccaa tatcctggac ggcacagtca ctgcaaccag ttccgatgcc attaacggta
     3121 gccagcttta tgacttaagc agcaatatcg ccacctactt cggcggcaat gcttctgtga
     3181 atactgacgg tgtgtttacc ggtccaacct acaaaatcgg tgaaacaaat tattataacg
     3241 tcggcgatgc actggctgcg attaactcct catttagcac gtctctcggc gatgctctgc
     3301 tttgggatgc caccgcaggt aaattcagtg ccaaacacgg tactaatggt gacgcaagcg
     3361 tgatcactga tgtcgcagat ggtgaaattt cagactccag ttctgacgca gtaaacggct
     3421 cacaactcca cggcgtgagc agttatgttg ttgatgcgct gggggggggg tgccgaagtc
     3481 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3541 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3601 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3661 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3721 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3781 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3841 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggatgagac tgccaatggc
     3901 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     3961 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4021 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4081 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4141 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4201 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4261 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4321 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4381 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4441 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4501 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4561 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4621 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4681 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4741 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4801 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4861 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     4921 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     4981 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5041 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5101 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5161 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5221 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggcgtgcg ctacgacacc
     5281 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5341 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5401 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5461 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5521 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5581 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5641 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5701 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5761 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5821 tctgatgagg attgcccttt tctttatcag acatctcccc ccacaagaat tggccctacc
     5881 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     5941 gccaacacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6001 agcgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6061 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6121 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6181 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6241 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6301 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6361 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6421 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6481 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6541 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6601 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6661 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6721 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6781 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6841 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     6901 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     6961 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7021 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7081 gtgggtgatc aacattccgg tgccgtacct cgataaactg gttgcccgta tgccaccagt
     7141 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7201 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7261 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7321 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7381 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7441 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7501 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7561 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7621 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7681 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7741 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7801 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7861 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     7921 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     7981 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8041 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8101 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8161 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8221 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8281 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8341 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8401 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8461 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8521 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8581 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8641 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8701 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8761 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8821 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     8881 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     8941 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9001 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9061 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9121 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9181 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9241 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9301 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9361 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9421 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9481 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9541 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9601 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9661 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9721 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9781 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9841 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
     9901 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
     9961 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgata
    10021 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10081 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10141 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10201 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10261 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtagtgt
    10321 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10381 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10441 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10501 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10561 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10621 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10681 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10741 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10801 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10861 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    10921 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    10981 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11041 ttcaaccact tcacggatag aaatagcagg cataattggc gatgacagct tgttcgccag
    11101 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11161 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11221 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11281 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtgg ctgctgcgct
    11341 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11401 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11461 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11521 tttgtacatc catgccctga ccgagcatca tgccaaaacg gcggttacgc gactggttgt
    11581 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11641 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11701 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11761 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11821 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    11881 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    11941 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12001 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12061 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12121 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12181 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12241 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12301 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12361 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12421 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12481 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12541 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12601 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12661 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12721 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12781 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12841 atactgcgca atgtaatgcc aacgttcaca cgttatttca gcaggggatc cagtccacca
    12901 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    12961 tggggaaccg tggtgcgacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13021 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13081 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13141 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13201 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13261 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13321 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13381 tgcatgccag aaccgtctac cacgataacc ggtttgtctt tgtgcttctc aagctcacca
    13441 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13501 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13561 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13621 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13681 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13741 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13801 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13861 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    13921 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    13981 gatggctatg gctatcgtga agaacaacag gataacgcca tttttagtgc taaaaccccg
    14041 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14101 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14161 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14221 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14281 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14341 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14401 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14461 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14521 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14581 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14641 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14701 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14761 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14821 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    14881 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    14941 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15001 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15061 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15121 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15181 gcggttaaag cagttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15241 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15301 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15361 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15421 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15481 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15541 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15601 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15661 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15721 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15781 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15841 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    15901 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    15961 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16021 caaacgcgtg aagaagtcac catgcagcgt gctgaactgg aagagaaaca aagcgagcaa
    16081 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16141 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16201 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgcgga agccgcggcg
    16261 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16321 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16381 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16441 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16501 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16561 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16621 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16681 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16741 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16801 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16861 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    16921 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    16981 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17041 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17101 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17161 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17221 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17281 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17341 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17401 attggacatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17461 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17521 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17581 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17641 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17701 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17761 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17821 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata ccggaagtaa
    17881 atatctcggc aatcatccgt tggcgggtaa gaatatccgg ctctttgcgc accgcatggc
    17941 aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc cggataaatc gtaattttgt
    18001 cggcataatt tcgatttaat ttctccagca ggcgggtaat cttaatatag tgacgttgat
    18061 aattaaggtt tttattccct tgtctatgca accgactcac tgacgtatta tgcagataat
    18121 atttatataa tgattgctcg gtatatcgcg cacgcagcgc gttaaacatg aattctgttg
    18181 tccagacaat atcctgatga tgtaatccgg caataaattt aatgttattt ttaacaataa
    18241 catcacggcg ataaaccccc atccagacaa cgtgagtcca acggcgcgaa aaaagcccca
    18301 tccgcagcca gtccgggccg gttaatacac cggttgagcg aaggcgatcg atggggatgg
    18361 attgccaagt ttctcccgtt tcacgaaaag accagtcagc gttgcactgc gccacgtcga
    18421 ggtcgtcctc taacgccatg gtcatcagcg tttcgtacat ggtgggatag acctcatcgt
    18481 cagcatcgac aaaagcgaca tatttgcccg tcgccacctc gatcccacga ttacgcgcta
    18541 ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg cggatagttt tctgcgtaat
    18601 gctttgctat ttcaacagaa ttatccgttg aaccatcgtt aataataatg atttccagag
    18661 cagtccaggt ttgcgtaatt aaagattcca tacaagtgcg gaaatcatcg cccgcattat
    18721 ataacggaat aataacacta agtttattgg tgctgttcat cataaattac cgacgacctc
    18781 tgaagttatc ctgacgtttt acaggctttg tattaagaaa ctattaagcc tgtgagatat
    18841 tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca gctatataaa
    18901 agatcagcgc aattacgaac tctttattac actgacagga
//
LOCUS       DADLSE010000076        18564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLSE010000076 DADLSE010000000
VERSION     DADLSE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.076"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(193..287)
                     /locus_tag="JN692_002544"
     tRNA            complement(193..287)
                     /locus_tag="JN692_002544"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Sec,seq:tca)
     gene            580..1963
                     /locus_tag="JN692_002545"
                     /pseudo
     CDS             580..1963
                     /locus_tag="JN692_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            1973..4291
                     /gene="yicI"
                     /locus_tag="JN692_002546"
     CDS             1973..4291
                     /gene="yicI"
                     /locus_tag="JN692_002546"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7264827.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFCFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(4344..6053)
                     /locus_tag="JN692_002547"
     CDS             complement(4344..6053)
                     /locus_tag="JN692_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7264828.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTSLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN692_002548"
     CDS             complement(6174..7565)
                     /gene="xanP"
                     /locus_tag="JN692_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7264829.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7845..9050
                     /gene="gltS"
                     /locus_tag="JN692_002549"
     CDS             7845..9050
                     /gene="gltS"
                     /locus_tag="JN692_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7264830.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN692_002550"
     CDS             complement(9084..11165)
                     /gene="recG"
                     /locus_tag="JN692_002550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7264831.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN692_002551"
     CDS             complement(11171..11860)
                     /gene="trmH"
                     /locus_tag="JN692_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7264832.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAAQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN692_002552"
     CDS             complement(11867..13975)
                     /gene="spoT"
                     /locus_tag="JN692_002552"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7264833.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN692_002553"
     CDS             complement(13994..14269)
                     /gene="rpoZ"
                     /locus_tag="JN692_002553"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7264834.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQESAELQAVTAIAEGRR"
     gene            complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN692_002554"
     CDS             complement(14324..14947)
                     /gene="gmk"
                     /locus_tag="JN692_002554"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7264835.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15205..16887
                     /gene="ligB"
                     /locus_tag="JN692_002555"
     CDS             15205..16887
                     /gene="ligB"
                     /locus_tag="JN692_002555"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7264836.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(16884..17501)
                     /locus_tag="JN692_002556"
     CDS             complement(16884..17501)
                     /locus_tag="JN692_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7264837.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN692_002557"
     CDS             complement(17792..>18564)
                     /gene="dinD"
                     /locus_tag="JN692_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7264838.1"
                     /translation="AEQWSARQLGKLLGYSEYRHFIPVLTRAKEACENSGHTIDDHFE
                     EILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVIAAGQTYFTIQTRRQELAD
                     DEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATATDFAIFQNHGYQGLYGGLD
                     QKAIHQLKELKKSQKILDHMGSTELAANLFRATQTEEKLKRDSVNSKQQANTTHFDVG
                     SKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
BASE COUNT         4242 a         4705 c         5011 g         4606 t
ORIGIN      
        1 ccgttgttat gtatcacatt tttcctatta tgggggtgct tgtatttcaa taggaaataa
       61 caccccctaa agtaccccca aaagaaactt gatacggggt gaagtaggtt gacctcagga
      121 tacaaataaa tactgatata caagggatta gaagagattt attgacattg ggagagttca
      181 gaagaggtaa actggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc
      241 caactggatt tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc
      301 tacatggagg cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt
      361 tttttccgcc taataaacgc agcgtattat cacgaaagca ttataattca tataaaaccc
      421 atattttaca atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc
      481 ataaattaat aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt
      541 tatcggtctc agcaccccta tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc
      601 gttaaagaga aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac
      661 gtaatgttat atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc
      721 ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg
      781 ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca
      841 ctgccattcg ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacggc
      901 aaaatgatct atgcagcaat tacttacacc ctaacttacc ttactttata ccgtcgtcaa
      961 tatcccttac tgcgcattgg gtggtgtaat caccaatgac ccgactcagc gtatctcgct
     1021 gcaatcctgg cgttttgtgc tggcgaccgc gggaggcatg ctttctactg ttctgatgat
     1081 gccactggtt aatttaattg gcggtgataa taaaccactc ggtttccagg gcggtatcgt
     1141 ggtcctttcc gtggtggcat tcatgatgct ggcattttgt ttcttcacca ctaaagaacg
     1201 cgttgaagca ccacctacaa caacgtctat gcgggaagat ttacgtgata tctggcaaaa
     1261 cgaccagtgg cggattgtcg gtttactaac cattttcaat atcctagcgg tgtgcgtacg
     1321 cggtggggcg atgatgtatt acgtcacatg gattttgggc acgccggaag tgtttgtcgc
     1381 ttttctcacc acttattgcg tgggtaacct gattggttcc gcactggcaa aacctctgac
     1441 cgactggaaa tgtaaagtca ctatcttctg gtggacgaac gccctgctgg cagtgattag
     1501 cctcgcgatg ttctttgttc ccatgcaggc cagcatcact atgtttgtct tcatcttcgt
     1561 gattggtgtg ttgcatcaac tggtgacacc tatccagtgg gtaatgatgt ccgataccgt
     1621 cgactacggc gagtggtgca atggtaaacg cctgaccggg atcagttttg ctggcacgct
     1681 gtttgtgctc aaactggggt tggccttcgg cggcgctctt atcggctgga tgctggctta
     1741 tggcggatat gatgcggcag aaaaagcgca gaacagcgcc acgattagca tcattattgc
     1801 gctattcacg attgttccgg cgatctgtta tttgctgagc gcgattatcg ctaaacgcta
     1861 ctactcactc acgacgcaca atctgaaaac cgttatggaa cagctggctc agggtaaacg
     1921 ccgttgccag caacaattca cctctcaaga agtgcagaac taaggaacgg caatgaaaat
     1981 tagcgatgga aactggttga ttcaacctgg cctcaatttg attcacccgc ttcaggtatt
     2041 cgaggttgaa cagcaggata atgaaatggt ggtctatgct gccccccgtg atgtgcgtga
     2101 acgtacctgg cagcttgata cgcctttatt tacgctgcgc tttttctccc cacaggaagg
     2161 tattgtcggt gtgcggattg agcattttca gggggcgctg aataacggtc ctcattatcc
     2221 gctcaatatt ttgcaggatg tgaaggtcac aatcgaaaac acagaacgtt atgctgagtt
     2281 taaaagtggc aacttaagcg cgcgtgtcag caaaggtgag ttctggtcac tggattttct
     2341 gcgcaacggc gaacgtatta ccggtagtca ggtgaaaaat aatggctacg tgcaggacac
     2401 gaataatcaa cgcaattata tgtttgagcg gcttgatctt ggcgttggcg aaacagttta
     2461 cggtctggga gagcgcttta ctgccctggt gcgcaatggc cagacggtag agacctggaa
     2521 ccgggacggc ggcacaagta ctgaacaggc gtataaaaat atcccgttct acatgactaa
     2581 ccgtggttat ggggtactgg tcaatcatcc ccagtgtgtc tcttttgaag tgggatcgga
     2641 gaaagtctcc aaagtgcagt tcagcgttga gagtgaatat ctcgaatact ttgttatcga
     2701 cggcccgacg ccgaaagcgg tacttgatcg ttatacccgc tttactggtc gtccggcgct
     2761 gccgcccccg tggtccttcg gcctgtggct aaccacttca tttaccacca actacgacga
     2821 agcgacggta aacagcttta tcgatggtat ggcggaacgc aatctgccgc tgcatgtttt
     2881 ccactttgac tgtttctgga tgaaagcctt ccagtggtgc gattttgagt gggacccgct
     2941 gactttccct gacccggaag ggatgatccg ccgcctgaaa gcgaaagggc tgaaaatctg
     3001 cgtctggatt aacccctata tcggtcaaaa atcccccgtc tttaaagagt tacaagagaa
     3061 aggctattta ctcaaacgcc cggacggttc gctatggcag tgggataaat ggcagccagg
     3121 tctggcgatt tatgacttta ccaatccgga tgcctgcaaa tggtacgccg acaaactgaa
     3181 aggtctggtc gcgatgggcg ttgattgctt taagaccgac tttggcgaac gtatcccaac
     3241 tgatgttcag tggtttgacg gttccgatcc gcagaaaatg cataaccatt atgcgtacat
     3301 ctacaacgaa ctggtgtgga acgtgctcaa ggacaccgtt ggtgaggaag aagctgtctt
     3361 gtttgcccgc tcggcctccg tcggtgcgca gaaattcccg gtacactggg gtggcgattg
     3421 ttacgctaac tacgaatcaa tggcggaaag cctgcgcggt ggtttgtcta ttggcctttc
     3481 aggtttttgc ttctggagcc acgatatcgg cggctttgaa aataccgctc cggcgcacgt
     3541 ttacaaacgc tggtgcgcgt ttggtttgct ctccagccat agccgtttac acggtagcaa
     3601 atcttatcgt gtgccgtggg cctacgatga tgagtcctgt gatgtggtgc gcttcttcac
     3661 gcaactgaaa tgccgcatga tgccgtatct gtatcgtgaa gctgcgcgtg cgaacgcgcg
     3721 gggtacgccg atgatgcggg ccatgatgat ggagttcccg gacgatccgg cttgtgatta
     3781 ccttgaccgt caatacatgt taggcgacaa cgtgatggtt gcgccggtgt tcactgaagc
     3841 gggcgatgtg cagttctacc tgccggaagg tcgctggaca cacctgtggc acaacgatga
     3901 actcgacggt agtcgctggc ataaacagca gcacggcttc ctgagtctgc ccgtttatgt
     3961 gcgtgataac actctactgg cgctgggcaa caacgatcaa cgtcccgatt acgtgtggca
     4021 cgaaggcacg gcattccacc tctttaatct gcaagacggg catgaagccg tctgtgaagt
     4081 gcccgctgct gacggatcgg tgatctttac tttaaaagca gcacgtactg gcaacacgat
     4141 tactgtgact ggtgcgggcg aggcgaagaa ctggacgctg tgcttgcgca atgttgtgaa
     4201 agtaaatggt ctgcaaggcg gttcgcaggc tgaaagtgag caggggctgg tggtgacgcc
     4261 tcaagggaat gcgctgacaa ttacgttgta atctattgac ggaatgccgg gtacgtctga
     4321 gtcgtacccg gcgagattga tgtttaccgc accggctccg tcgatgtaac ttcaccgctg
     4381 gaaacgacgc ccgcattcat ggtttgagtc acttgctgct ttgcggcgtt cacggcatgc
     4441 aattgcccgc taaccgtagg tttcagcggg gcattggcct gaatatctcc actggcggta
     4501 agctgaatat tgccgtcgcc agtcaacggt aattcaggcc atccccattg ttgcaaaata
     4561 ttcaccggca caccgcgtcc attcaggctg atatgggtct gacgttgtgg cgtttgtgaa
     4621 acacttgcgg tggcttccag aatgcctttt tcagtaaatg cactcagttc gctgatattc
     4681 accgtgctgc tgttggcggt cagcgccagc gacgggcgac gaacatcaac acgattgaat
     4741 gtcgcggcgg cggcattcag attcgccgag ccactccaga cgccccattt atgatcggta
     4801 accagcgtca ggttggcacc gtaaccatcg agcgaggtga gctgccacgg gaagtcaggg
     4861 tcgatatcaa tgatcagatt gcggctggcg ctaaatctct tcagttgcag gctgtttaac
     4921 caaccgggtg tcgtttccat ccacaactgt tgccagtttt tcggcaaggt atattccagc
     4981 ccggcaattg ccgcatcatc aaggatcaac gttttaccgt cacgcagcca gttccctgac
     5041 gttctgacca tacccccttc ccagcggctg gtgaactggc gcagcgctac gccctgcggg
     5101 gaaaattccg cgtttataat cgggtcaaat aaatgcagcg aaccataaat gaactcgctg
     5161 gcgttcatcg acagtttgcc ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg
     5221 cgcaagctga gatcgaggtc ggtcaccgcc cagtccggac cttgcagacg agcatcgatg
     5281 acttccaagc gaccaatctg caacgacggg acagagcgta atggcgcaaa gaagtcggtt
     5341 agcgattttt cgctttgtag acggatatcc gccatgcgca gattttccac ttgccagctg
     5401 ccgtcggcgt tacgctgcgc gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg
     5461 ttagtcagcg taacgcgatc gttatcaata ctgccttcaa tcagtacatt ggtggcagga
     5521 acatcattga gcgaaagcga tccggcacta aactgaatct gcgccttcgt acccagcact
     5581 ttaccggctt ctggtgacca cggaaccacg ccgccattta cccgctgcgc gctcagtttc
     5641 cattcgctat tcgggctatt aaacgccata tcacgcagtt gcagacgatc ggctttgaac
     5701 ggtagcggcg cggtctggtc ggtgagattc agcgtcccgt tttccagcag gatggtatcg
     5761 acatggcgtg gttcggtcag ttggcgactg cttagcgcaa tgtcgacact ttttgccacc
     5821 aggggcgcgg gctgaccatc acgaccaaac gtgacattct ccagcacgat atgagatggc
     5881 gcggaaaaac ggtgatccat cgccccgaag gccagatgat agtcgctatt ctcggaaacc
     5941 catgcgctga tatgttctgc tccccagcgg gtttgcagaa gaaaatagag gccagcgatc
     6001 accactaaca gagcgatgag aatgtagaga agcagcttcc caataaattt catggtcttc
     6061 catcccgtga aatgcacata agggagttat gcacgattta cgcgcaatcc tcaaggcggg
     6121 aatggtgaaa gaggtgagac tgcggcggta attatcttac cgccgccagt gaattactgt
     6181 ttttctggtg ggaaaatcag attcagcacg atggcagtaa taccgcccgc ggcgatcccg
     6241 gaggagagca ggtttttcag ccattcaggg gcaaactgca aaatcagcgg ctgctgagac
     6301 acgcccagac caaccgccag cgacagcgcg ataatcagaa tcgcccgacg gttcagcggc
     6361 tcacgagaaa cgatacgcac accggaggcg gcgatggtgc caaacattac aagcgttgcg
     6421 ccgcccagaa ccggttctgg aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc
     6481 agcacgatca acatcagcgc gacgacaaaa ccgacatagc ggctggcaac accagtcaac
     6541 tggatcactc cgttgttttg cccgaagcag gagttcggga aggtgttaaa caccgccgaa
     6601 acaaacgagt tcaggccgtt tgccagcacg ccgcctttca ggcgtttcat gtacagcgga
     6661 ccggacactg gctgttcgga aacgtcagag gtcgccgtga tatcgccaat ggtttccagc
     6721 gaagtgatca taaagaccag catcagcggc agcagcagac tccattcaat gccaagaccg
     6781 taatagagcg gcgttggcac cataatcagt tcttgcgtca tcggttcgtt gctttctggc
     6841 aacatgccca taaaccacgc cagcgcatat ccggccgcca tcgcaattac cagtgaggcc
     6901 acgcgtaagt aagggttacg ttgacggtta agcaggataa ttaaggctaa aaccacgcct
     6961 gccagcagca gatttttcgg tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg
     7021 ccaatggagg tcagccccac ctgaattagc gacaggccga taatcatcac cacaacgcca
     7081 gaaaccagcg gcgtaataat gcggcgcgcc agatgcagaa cgcgggagat caccatctcg
     7141 gtgcaacttg ccagcatcaa cgtgccgaac aaagccgcca tcatggtagg aacatcagca
     7201 ccaccggttt tcagcgcggt accgcccata atcagcgggg caacaaagtt gaagctggtg
     7261 ccctgaatag acaacagccc ggagccaacc ggaccccagg ccttaatttg aataatcgat
     7321 gccacaccgg aggcaaacag cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa
     7381 cccagcgcct ggcagattaa tagcgctggc gtgatcaccg caacgaacat cgccagcaga
     7441 tgctgacagg cggcaaacag ggtttgagga agcggcggac gatcttcaag acggtaaatc
     7501 agttcgctgt tttgagtctg cgcaaccggt tgcgcatttt ctgactcgag ggtggaaaca
     7561 gacatcgaaa acaatcccgt ggtagcaaag cgggcatttt agctgaccga aagagaaaag
     7621 caaacgtttg ccacatcctc atctaaatat ttaaatgaga taaatgcact tttttatata
     7681 acttttggta tttttctgct taaaatccat gccatttgtt cattaacagc tgagaatttg
     7741 acccaggaaa gcataactcg atacccgtca tagttcatga agttgccact gcgcgatgaa
     7801 gtatgacgag tatgaaagag tgatgcggat acaaaggagt aactatgttt catctcgata
     7861 ctttagcaac gcttgttgcc gcaacgctga cgttgctgct cgggcgtaag ttggtccatt
     7921 ccgtctcctt tttgaagaaa tacaccatac cggaacctgt tgcgggtggt ttgttggtgg
     7981 cgctggcgct actagtactg aaaaaaagca tgggctggga agtcaacttt gatatgtccc
     8041 tgcgcgatcc gttaatgctg gctttcttcg ccaccattgg cctgaacgcc aacattgcca
     8101 gtttgcgtgc cggtgggcgt gtggttggca tcttcttgat tgtggttgtt ggtctgttgg
     8161 tgatgcaaaa tgccattggc attggtatgg ctagcctgtt agggcttgat ccgctgatgg
     8221 ggctgttggc cggttctatt acgctttccg gcggtcacgg tacgggcgct gcgtggagta
     8281 aattgttcat tgaacgttat ggcttcacca atgcgacgga agtggcgatg gcctgtgcaa
     8341 cgttcggtct ggtgctgggc ggcttgattg gcggtccggt ggcgcgctat ctggtgaaac
     8401 actccaccac gccgaacggt attccggatg accaggaagt cccgacggcg tttgaaaagc
     8461 cggatgtggg acgcatgatc acctcgttgg tgctgattga aactatcgcg ctgattgcta
     8521 tctgcctgac ggtggggaaa attgttgcgc aacttttggc tggcactgct tttgaactgc
     8581 cgaccttcgt ctgtgtactg tttgttggcg tgattctgag caacggtctg tcaataatgg
     8641 gcttttaccg cgtctttgag cgtgcggtat ccgtgctggg taacgtaagc ttgtcgttgt
     8701 tcctggcgat ggcgttgatg gggctgaaac tgtgggagct ggcttcgctg gcgctgccga
     8761 tgctggcgat tctggtggta cagaccatct tcatggcgtt gtatgccatc ttcgttacct
     8821 ggcgcatgat gggcaaaaac tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg
     8881 gcctcggtgc aacgccaacg gcaatcgcca acatgcaggc gatcactgaa cgctttggcc
     8941 cgtcgcacat ggcgtttttg gtggtgccga tggtcggtgc gttctttatc gatatcgtca
     9001 atgcgctggt gattaagctg tatttaatgt tgccgatttt tgccggttaa ccgatgaagc
     9061 ggcggtcttc tcaccgccgc cttttacgca ttcgagtaac gttctgtctc cggcatccag
     9121 cgttctatca aggcttttgc ctgttgtggg taacgttcgt gaatatgtcg cgccaggcgc
     9181 tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa ttcagcatta
     9241 cccgtctgac gcgtgcctaa caattcgcca gggccgcgaa tctccagatc tttttgcgca
     9301 atcacaaaac cgtcgttact gtcgcgcagc acttgcaggc gaatttgcgc cgttttagaa
     9361 agcggcgttt tgtagagcag cacgcagtga gaagccaccg cgccacgacc tacgcgcccg
     9421 cgcagctggt gtaactgcgc cagacccaga cgctcagggt tttcgataat catcaggctg
     9481 gcgttaggca catcaacacc gacttcaata acggtagtgg caaccagcag atgtagctca
     9541 ccttgtttaa acgacgccat caccgcctgt ttctcggctg gtttcatccg cccgtgtacc
     9601 aggccaacat tcaattctgg tagcgccagt ttcaactctt cccaggtagc ttccgccgcc
     9661 tgcgcttcca gtaactctga ctcttcaatc aacgtacaaa cccagtacgc ctgacgacct
     9721 tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt atcaggaata
     9781 gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga ggtatcgaga
     9841 tcggcatacg ccgtcattgc cagcgtgcgg gggatcggcg tggcggtcat gatcaactga
     9901 tgcggatgga agccctgctg ctggcctttc tcccacaatg ccagacgctg atgcacgcca
     9961 aaacgatgct gttcgtcgat aatcaccagc gccaggccgt taaactgcac ctgttcctgg
    10021 aagatggcgt gtgtaccgac aatcatctgc acctgaccgc tggcgatggc ttcctgctga
    10081 gacagtcgcg ctttgccttt ctgcttaccg gcgagccagc ccacttcgat accgagcggt
    10141 gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga gtaattcggt tggtgccatc
    10201 agtgccacct gtttgccgtg ggcaatcgca cgcagcgcgg cgagggcggc gaccagcgtt
    10261 ttaccggaac ctacatcgcc ctgtaccagg cgcatcatcg gcacatccag cgccatatca
    10321 tgctcgatct ccgccactac gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg
    10381 aggagtttat ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa acgctgtgct
    10441 ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag aatcagacga
    10501 cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg tggcgggcgg
    10561 tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc cggcggcagg
    10621 agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc
    10681 gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc ctgtaattct
    10741 ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc accatattta
    10801 ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct atttttcatt
    10861 gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct gatctggcag
    10921 gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc ttccaccgtg
    10981 gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc
    11041 aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc cagtttgtta
    11101 ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa caggcgacct
    11161 ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca gcttcgatct
    11221 cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact ttcgccagca
    11281 ccggatagcc gccttcaaac aacaggcgtt gttgcgctgc ttccggcaac atgctgtttt
    11341 cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga atgagggctg
    11401 aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg atgtcctgat
    11461 ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc aaaatgcagg
    11521 tcgggcgagt gtaatcaatt tcgcggaaat cgacagcatt atcagaaaga tgggttgcca
    11581 gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg cggtgtgttt
    11641 tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc atgcggctac
    11701 caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga ataatcgcag
    11761 aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc tgccgcctgg
    11821 cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa tttcggtttc
    11881 gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc gccagatgca
    11941 cacggtcacg agcggtcaga cgaataaagg cgctgtagac gcgaccatct ttctcttccg
    12001 tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg tttgccagcg
    12061 cgccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc gccgtctctt
    12121 tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg atattacggc
    12181 aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata atcgggtcgc
    12241 caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatcggca cctttaatgg
    12301 gcagatgtcc gtggctttgg gttgccggtg gaatggaggc gtccccatgt tgcagatttt
    12361 tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc tgccagcaga tcgtcaagcg
    12421 ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt tcattcagct
    12481 tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca gaatcatcac
    12541 gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc ttttgagcta acgacaaagt
    12601 tcagccacgc ggcattcggg cgagcgcccg gtgcggtaat gatttcaacg gtttgaccgc
    12661 tggtaagcgg ctgcgacagc gggtaaggct ggcggtcaac gcgtgcgccc acgcaggcat
    12721 gaccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg gcaggcagct
    12781 cgacaattcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa
    12841 cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc aggctttgca
    12901 tccagcgctg ggcgcggatt tgcgcggtgg tgctggtttc gccgtgctct ttataagccc
    12961 agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg atctggacct
    13021 caaccggcac accgtgcggg ccgatcatcg aggtgtgcaa agactgatag ccgttcgctt
    13081 ttggaatggc gatgtagtct ttcacgcgac ccggacgcgg cttgtacagg ctatgcattt
    13141 gcccgagcac gcgataacag gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt
    13201 ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc gaataaagat
    13261 gcttctcgcg accactgacg cggcagggaa ttcccgcttc ctgcaaacgc ccttcgattt
    13321 ccgagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc accacttctt
    13381 tgattacgcg ataacggttg ggatacagcg cctcaaaacc cagctcttcg agttcggttt
    13441 taatgtggtg gatacctaaa cggtgcgcca gcgggctgta aatttcgaga gtttcacggg
    13501 cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt
    13561 cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc atcttgcgaa
    13621 agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt ttcgacaccc
    13681 cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg taggtggcgg
    13741 gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag tcgagtttca
    13801 tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca ccgcttgaac
    13861 gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt
    13921 ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga tacaagggcg
    13981 acccgctttg tgattaacga cgaccttcag caatagcggt tacggcttgt aattcagcgg
    14041 attcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg ttgatcagac
    14101 cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc ggtaccagcg
    14161 gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa
    14221 aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata cttaaaaagc
    14281 tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct gccaacaatt
    14341 tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa
    14401 taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc agataatcat
    14461 attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca atgacctctt
    14521 cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac ggcggtaaaa
    14581 taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga
    14641 tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc
    14701 catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta atcatttctt
    14761 taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca ggacgcggtt
    14821 ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt tttaataaag
    14881 cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa agcgtgcctt
    14941 gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg agcttattat
    15001 acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt
    15061 ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat tctcaggaag
    15121 cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg tatatctgcc
    15181 agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat aagtatcttg
    15241 tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag agcacaggaa
    15301 gaaatttccc gcctgcaaca gcaaataaaa cagtgggacg atgactactg gaaggaagga
    15361 aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac gcagtggcaa
    15421 cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg
    15481 catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg
    15541 atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg
    15601 gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag
    15661 gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag cgggccttta
    15721 gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat ccaacaacaa
    15781 atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca ggggaatagc
    15841 gacacgctaa actcactggc tgtttttgtc tgggcatggc cggatggacc gcagttaatg
    15901 accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag gtatacccgt
    15961 gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa agcgaaatta
    16021 cccttcgtca ccgatggcgt ggttgtgcga ggggctaagg aacccgaatc ccgtcattgg
    16081 ctaccgggcc aggcggagtg gctggtggcc tggaaatatc aacctgtagc tcaggttgcc
    16141 gaagtgaagg cgattcaatt tgcggtgggt aagagcggta aaatatcggt ggttgcgtca
    16201 ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg
    16261 cgctggcagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag
    16321 ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa accgacacca
    16381 ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg tcaggaacag
    16441 ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga tggcattggt
    16501 gaggccggtt ggcgcgcgct gcatcagact catcgctttg agcatatctt ttcctggctt
    16561 ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag
    16621 ttatggcatc agtttaatct ggctcgtaac cagcctttta ctcgctgggt gatggcaatg
    16681 ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt
    16741 ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatctgg gcgcgccaga
    16801 caggttattg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc
    16861 cagcagatca caggttttga accttagtga ccttcgtggc tgtagtaaaa gaccggtaat
    16921 cccagcttaa gacgtagcgc cagtaaacga gcaaaaaagc cgaatactag ggtggagata
    16981 atcaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac ggcggaggca
    17041 aatgagacac cggcatacaa ctctttttga aagaccagcg gaatgcgttt acagaacata
    17101 tcgcgcaaaa cgccgccaaa tacaccggtg gttaccgccg cgacaacggc aataattggg
    17161 ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac cagtccgagc
    17221 gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac gatagtggtg
    17281 agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca gccgagcgga
    17341 tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc ggttgcaata
    17401 ataattacgc caaatgtatc catgcgccgt cgcccggccg ccagcgcccc cgtcatggct
    17461 tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc cctaaaaatc
    17521 tgtctggcag ggtagcgatt tgtgcgcatt gtcacgattg atatttttta aggtgagagg
    17581 aaattgataa cttttagtta tgtgaaatcg ctattttctg tagcgtagat gcattcttct
    17641 gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaactgaggg gggaaaagaa
    17701 aggtgcaatg tttagccatt gtgttcactg cacctggacc ttggcttacg catcgggtgt
    17761 ctcaatcaac aaaaagagtt tttcgtttct gctatttctt ctctgtaatt tttacactgt
    17821 tttccaactg cttgatgctg acctgcgggg tcggcaactc ctcaggcatg gttccgccaa
    17881 gttcctgaat ggtttgcctc accttgctac ccacgtcaaa gtgtgtggta ttagcttgct
    17941 gttttgaatt cacgctatcc cgcttgagtt tttcttctgt ttgggtagct cgaaagagat
    18001 tagccgccag ttctgttgaa cccatatgat ccaggatctt ctgactcttt ttcagctcct
    18061 tcagttggtg gatggctttc tgatctaatc caccatacag cccctggtaa ccatgattct
    18121 gaaatatggc gaaatcagta gctgtcgcta ccccagcttg ctgtgctgcc tcaaccaatt
    18181 gtttgttatg ttctttcaac tcatttctca gaaacagacg cttttcgtct tcacgaagtt
    18241 gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt ctggatagta aaataagtct
    18301 gccctgccgc aatgaccggt ttcgcggggt cgccgttttg tactaccagg taacaggcat
    18361 agcgggagag tacgatgtct tttaatgctc ttttggcatt tgagccaatt ttgaccatat
    18421 cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact gttctcacag gcctctttgg
    18481 cgcgcgttaa tacaggtata aagtgacgat actctgaata acccagtagt ttcccaagtt
    18541 gtcttgctga ccattgttct gctc
//
LOCUS       DADLSE010000077        18528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLSE010000077 DADLSE010000000
VERSION     DADLSE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.077"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..419)
                     /locus_tag="JN692_002558"
     CDS             complement(<1..419)
                     /locus_tag="JN692_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7264839.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLG"
     gene            526..822
                     /locus_tag="JN692_002559"
     CDS             526..822
                     /locus_tag="JN692_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7264840.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN692_002560"
     CDS             complement(956..1381)
                     /gene="arsC"
                     /locus_tag="JN692_002560"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7264841.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN692_002561"
     CDS             complement(1394..2683)
                     /gene="arsB"
                     /locus_tag="JN692_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7264842.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLSMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN692_002562"
     CDS             complement(2737..3090)
                     /gene="arsR"
                     /locus_tag="JN692_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7264843.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            3833..3916
                     /locus_tag="JN692_002563"
     CDS             3833..3916
                     /locus_tag="JN692_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7264844.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN692_002564"
     CDS             complement(3970..5322)
                     /gene="gorA"
                     /locus_tag="JN692_002564"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7264845.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5394..6236)
                     /locus_tag="JN692_002565"
     CDS             complement(5394..6236)
                     /locus_tag="JN692_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7264846.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6439..8481
                     /gene="prlC"
                     /locus_tag="JN692_002566"
     CDS             6439..8481
                     /gene="prlC"
                     /locus_tag="JN692_002566"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7264847.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN692_002567"
     CDS             8489..9241
                     /gene="rsmJ"
                     /locus_tag="JN692_002567"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7264848.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN692_002568"
     CDS             complement(9290..10759)
                     /gene="dtpB"
                     /locus_tag="JN692_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HAY7264849.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN692_002569"
     CDS             complement(11077..11511)
                     /gene="uspA"
                     /locus_tag="JN692_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7264850.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11902..12237
                     /gene="uspB"
                     /locus_tag="JN692_002570"
     CDS             11902..12237
                     /gene="uspB"
                     /locus_tag="JN692_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7264851.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN692_002571"
     CDS             complement(12308..13807)
                     /gene="pitA"
                     /locus_tag="JN692_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7264852.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14039..15238
                     /locus_tag="JN692_002572"
     CDS             14039..15238
                     /locus_tag="JN692_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7264853.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKIPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSQKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
     gene            16404..17915
                     /locus_tag="JN692_002573"
     CDS             16404..17915
                     /locus_tag="JN692_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HAY7264854.1"
                     /translation="MPLQQFSRLALTLAIGLSIAAGGAALTWYQVAETDLSRTAAAAH
                     EAVSVTERLLDEAQGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLS
                     CSSWEGADPVREFISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVA
                     DNMTLLNLRRPLSLRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPL
                     TLYFHDGALSLLLSVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDA
                     STGEIAGVEVLARWVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLLPLTGD
                     GKRWHISFNITQSHIMETGFIAECLTFIDAFTPGSITLTIELTEREPFDNSDEMRCQL
                     KRIHDAGIALALDDFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIE
                     MANTLKKEIIAEGVETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT         4680 a         4576 c         4786 g         4486 t
ORIGIN      
        1 cccagagaat ctaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
       61 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      121 aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc cggctttcgc tgacaacaat
      181 ccacgtaaca tgggtattgt cgagcaacta cagaacgggg taacggctcc caacagagca
      241 gccaggagat agcctctccc ttttctggcc cccatcatct gctggatttt atggtctggc
      301 accttttgtc ttatcaggct gacaccggca ctaatcacta taaacagcaa cgaaagctcg
      361 acggcgagaa acacaaacat ctctgcggca tcttgcagca tcgcaagcca actactcata
      421 cgtaacctca ttggataaat ctatatttcc agaataatcg aaatgaaatt cagatcaaga
      481 tatttctggt attatcgaaa tatcaaccag acagaggggt gtcatatgca gttggaagaa
      541 gtcgctaaag cgctgaaaga gctaggtcac ccgacccgtt tgttcatatt taaacatctt
      601 gtcaaagccg gggaacaagg gttgccggtc ggtgagttgc aaaagcagct tgggatacct
      661 ggctccacac tcagccacca tatatcagca ctggtatcgg taggactggt caaacaaaac
      721 cgtgagagtc ggacgctgat gtgtgtttcg caatacgcaa tgctggaagc gataattgaa
      781 ttcttacgcg aggagtgctg cgtaaatagc aaaattgcct gatgcgctac gctctacata
      841 tttcatgcca tatattgaat ttgcacggtt ttgtaggtcg gataagacgc gttagcgtcg
      901 catccgacat aaacaaagcg cactttacca actatcggca gtggatatcg ccgctttatt
      961 tcaggcgctt acccgcttca tcaaccactt tctcgccatc ttccttggag aatgcgcctt
     1021 tttgcgcatc tggcagaatt tccagcacca cttctgaagg gcggcacagg cgagttccca
     1081 gcggcgtcac cacaatcggg cgattaatca gaatcgggtg ctgaagcata aagtcgatta
     1141 accgatcgtc agtaaattta tcttccgcaa ggcccagctc ctcatacggt tcgacgtttt
     1201 tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat gagtttgacc agttcatcgc
     1261 gcgttggcgg agtttccaga taatggataa tggtcggttc tgtgccgctg ttgcggatca
     1321 tctccagcgt attacgcgac gtgccgcagg ccgggttgtg ataaatggta atgttgctca
     1381 tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc cagcgcagcc agcgtcacaa
     1441 acagcacagg cagggtcatg ataatccctg tacggaagta atatccccag ctgatagtca
     1501 tattcttctg cgaaagtacg tgcagccaga gcagcgttgc caggctacca atcggggtaa
     1561 ttttcggccc caaatcgcag ccaatcacgt tggcataaac catcgcttct ttgataacgc
     1621 cagatgccgt gctgccatca atggacaacg cgccaaccag taccgtcggc atattgttca
     1681 taatagaaga gaggaaggcg gtgaggaatc cggtgccgag cgtcgcggcc cacaggccgt
     1741 tatccgccag cacgttgagt acgccagaaa gatattccgt taatccggca ttgcgcaggc
     1801 cataaaccac cagatacatg ctgagcgaga agatgacaat ctgccagggg gcaccgcgca
     1861 ggactttacc cgtattaatc gcatgaccgc gtttagcgac gacaaataat atcagtgcgc
     1921 ccacagctgc aatggcgctc accggaatgc cgagcggttc caagacgaaa aatcccacca
     1981 gcagaagcag taaaacaacc cagccagttt tgaacgtagc aggatctttg atcgcttctg
     2041 cgggagattt cagcagcgcc atatcgtagt tctgcggaat atctttgcga aaatagagat
     2101 gtagcatcac cagcgtggca acaatcgcgg cgatatccac cggcaccatc accgaggcgt
     2161 attcgcgaaa gccgaggcca aagaaatcag cggaaacgat attcaccagg ttggagacaa
     2221 taagcggcag gctggcggta tcggcaatga atccggccgc catcacgaac gccagcgtcg
     2281 tgcctttact gaatcctaaa gccagcagca tggcgatgac aatcggcgtc aaaataagcg
     2341 ccgcgccatc attggcaaac agggccgcaa cggcggcacc gagcaggaca atccaggtaa
     2401 acagcaagcg accgcgtcca ttgccccagc gtgaaacgtg cagcgctgcc cattcaaaaa
     2461 agccggactc atccagcagc aggctgatga taatgacggc aataaacgcc gccgtcgcgt
     2521 tccagacgat attccacacc accggaatat cacccggatg aaccacgccc gtaaccaacg
     2581 ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa acctttcggc tgccagataa
     2641 ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa cataatgcct cccggataaa
     2701 acacatgtgc aaatccatat gtgtttagct aaatttttaa ctgcaaatgt tcttactgtc
     2761 tccggaacag ttttgtctgg ccaggttgcg gacaattgcc tgaatctttt cctgttcaca
     2821 tcgccaggct tcatcaataa ttttcgccgc ccatgctgga atatgcggcg ataagcggta
     2881 atgaacccac ttaccttgct tgcggtccag caatagcccg ctttcacgca gcaatgccag
     2941 atggcgggat atcttgggct gcgactgatc gagggcagtg cagagatcgc agacgcataa
     3001 ctctcccagt tcgctaagca gtaaaacgat gcccagacgg gtttcatcag caagaatttt
     3061 gaacaattgg atgggtaaca gaaatgacat attgcgctcc tgattgttgc aggtagtgtc
     3121 tctcttcgaa gcggataagt caaaaacata tatgacttaa cgaatgtgta agtgcagagg
     3181 aaggtaatag gtgtgaattt tgagttggct attcatttga aaggcggttt gaatcaggac
     3241 gtaaaaaaac cgactttgcg tcggtttttt actcttcagc ctgagttggt ggctctggct
     3301 ggagtgagaa tgcccacatc taacggttca atactattag ccagtggcta acattgcaag
     3361 cgttagcgat ttcttcagct ggagaaggga cagtttgcta tacatggtgg tgaaagagga
     3421 tttatatact ttcatcgggt cgctgccgta ccaagccttg tccgaatccg gacgtaaaaa
     3481 aaccgacttc gcgtcggttt ttttactctt cagcccagag ttggtggctc tggctggagt
     3541 gagaatgcca tcatctaacg catcaatgct aataccatac tgaaactatt gcaaggttat
     3601 gctggttatt tagcctaatt acactgtatg attatccagt tagctctgag gcattttcac
     3661 tctggcaatg cgcataaacg ctttcaaagt cctggtcaga agtacgggtg gtgccgttaa
     3721 ctgatgctct ggccggagtg agattgccca catctaacaa tgaggcatgc gccgtgacag
     3781 gcagtggatg agtaagcgga tgcattctca ctccatcgca tggagaaaac gggtgattga
     3841 taaagcaatc atcgttcttg gggcgttaat tgcgctgctg gaactgatcc gctttctgct
     3901 tcagcttctg aactgatagc ggaaacgtaa ttaagggcta agagcacact actcttagcc
     3961 ctttaacatt taacgcatgg tcacgaactc ttctgccgcc gttgggtgaa tggcgacggt
     4021 attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc accgcgaagc cctgcaacat
     4081 ttcgtccata ccaaagccaa tgccgtgaat accgacaatc ttctcttctg gcccaacgca
     4141 caccagcttc atgcggcacg gctggcggtg agtggtgacg gcggtataca tcgcggtgaa
     4201 agaggattta tacactttca cctgatcgtc gccgtactgc tcgcgcgcct gcggttccgt
     4261 taaaccaaca gtaccaatcg gcggatggct gaagaccacg gtcggaatgt tgctgtaatc
     4321 cagatgctca tccggcttgt tattaaacag gcgttcagag agacggcgac ccgctgcaac
     4381 tgccaccggt gtcagttcca ctgcacccgt gttatcgccc accgcgtaaa taccttcaat
     4441 attggtgttt tgatatttat cgacgacgat atagcctttt tcgttggttt taacgccagc
     4501 ggcttcgagg ttgatgttgt cattggcagg ctcgcgacca atcgcccaaa tcaggcaatc
     4561 caccgtttca ctgcgaccat cttccagctc cagcgtcagg ctaccatcgg catttttcac
     4621 taccgctttc gggatggcgt tggtatgcaa ctgtgggcct tcggcgttca tcacttcgac
     4681 cagcgtttca gagatcatcg ggtcgaagct gcgcagcggc gcatgtttac gcacaaacag
     4741 atgcgttttc gcgccgaggc cgttaatcac gccagccagc tcaacggcga tgtaacccgc
     4801 gccaacaacc gccacgcgct ctggcaaagc aggaagggcg aagaagccat cggaatcaat
     4861 accgtattcc acgcctggaa tatccgggtg gctcggacga ccgccggtgg cgatcagaat
     4921 atgatcggcc gtgatggttt cgccgttaac ttccagcgtt ttggcatcaa cgaagcgggc
     4981 aaagcctttg attacatcaa cgttattttt accgagcacg ttttcatagg aagtatgaat
     5041 acggtcgata taggcggtac ggctggcgat caacgtttcc cagttgaact tgttgatggt
     5101 ggtatcgaaa ccataatcag ggccgtacat atggatcgct tcacggattt gcgccgcgtg
     5161 ccacatcact tttttcggca cacagccgac gttcacgcag gtgccgccca gctctttggc
     5221 ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg ttgatggagg cgataccgcc
     5281 gctgccgccg ccgatggcga tgtaatcata gtgtttagtc atgacatatt gtccttatcg
     5341 ttgattaccg cgattgtagc gcgagacgta ataggtgcca gcaatggctg caattactcc
     5401 ggcacgatcc agcttacggt ggcgtgcccg gtacctgccg gaaccagttt gctgtgcagc
     5461 cacgacagca cgttattcat ctgctgttcc agcttccacg gcgggttaat cacaatcatg
     5521 ccggaagcgg tcatgccacg gcgatcgctg tctggcagca ccgccagttc aatttgcaga
     5581 attttgcgaa tgccggtcgc ttccaggtcg tggatcatgc gcttaatttg ctggcgcagc
     5641 accaccggat accacaatgc gtaagtgcca gtggcgaaac gtttgtaacc ttctgctatc
     5701 ccgctgacca ccgcctgata gtcggttttc atttcatacg gcgggtcgat aaggattaaa
     5761 ccacggcgag aaaccggcgg cagtttggcc ttaagttgct ggaaaccgtc ggctttttcg
     5821 acgcgtgcac ggctgtcttt ctgaaatcca gaacgcagca acgggtaatc gctcgggtgc
     5881 agttcggtca gttgcaggct gtcttgttcg cgcagtagct ggcgagcaat caacggcgaa
     5941 ccggggtagt aacgcaactg gccgctacgg ttgaagtgtt ttaccacatt gatgtacgcc
     6001 tccagttctg cgggcaaatc gtcctgctgc cagatacggg cgataccttc gagatattcg
     6061 ccggtacgct cggcatgttc gctacctaac tgataacgcc cggcccctgc gtgggtgtcg
     6121 agatagagaa acggtttatc tttctctttc agcgactcga tgatcaggct ctgaacggta
     6181 tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt ggcgataact gagcatgggt
     6241 aaaggtgttc cggtaagtaa aaattggccc gtattcgggg cgcacaaaag cctatcagga
     6301 cagtataccg aaaagaggcc gccgccgcga aagcgtaacg tttctcattg aaattcacta
     6361 cacttaaccc catgctacat acattatgta aagcgcctgt tgagcgcttc cttaacctct
     6421 ttaaccagga ctgcgctaat gacgaatccg ttactgactc cctttgaatt gcctccgttt
     6481 tctaaaattc tcccggaaca tgtcgttcca gccgtgacta aggcattgaa cgactgccgc
     6541 gaaaatgtgg agcgcgtagt agcgcaaggg gcaccgtaca cctgggaaaa tctctgccag
     6601 ccgttggcgg aagtggacga tgttttgggg cgtatcttct ccccggtcag ccacctgaac
     6661 tcggtgaaaa atagcccgga actgcgtgaa gcgtacgaac aaaccctgcc gctgctgtcg
     6721 gaatacagca cctgggtagg gcaacatgaa gggctgtata aagcgtaccg cgacctgcgc
     6781 gatggcgatc attacgccac gctgaacacg gcgcagaaga aagcggttga taacgccctg
     6841 cgtgattttg aactctctgg cataggtctg ccgaaagaga aacagcagcg ttacggcgaa
     6901 attgctaccc gtctttctga actgggcaac cagtacagca acaacgtcct cgatgcgacg
     6961 atgggctgga ccaaactcgt taccgacgaa gcggggctgg cggggatgcc agaaagcgcg
     7021 ctggctgcgg caaaagccca ggccgaagcg aaagagctgg aaggctacct gctgacgctg
     7081 gatatcccaa gctacctgcc ggtaatgacc tactgcgaca accaggctct gcgcgaagag
     7141 atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc cgaacgccgg taagtgggat
     7201 aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg aactggcgca actgctgggc
     7261 tttgaaaact acgcctttaa atcccttgcc actaaaatgg cagaaaaccc gcagcaggtg
     7321 ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc aaggcgaaaa agagctggag
     7381 caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg agttgcagcc gtgggatatc
     7441 gcttactaca gcgaaaaaca gaaacagcac ctctacagca tcagcgacga gcagctgcgt
     7501 ccgtacttcc cggaaaacaa agcggttaac ggcctgtttg aagtggtgaa acgtatttac
     7561 ggcatcaccg ctaaagagcg taaagatgtt gatgtctggc atccggatgt acgtttcttc
     7621 gaactgtatg acgaaaataa cgaactgcgc ggcagcttct acctcgatct gtatgcccgt
     7681 gaaaacaagc gcggcggggc gtggatggat ggctgcgtag gccagatgcg taaagctgat
     7741 ggttctctgc aaaaaccggt cgcgtatttg acttgtaact tcaaccgccc ggtaaatggt
     7801 aaaccggcgc tgttcactca cgacgaagtg atcaccctgt tccacgagtt cggtcacggc
     7861 ctgcaccata tgctgacccg catcgaaacc gctggtgttt ccggtatcag cggtgtgccg
     7921 tgggatgcgg tcgaactgcc gagtcagttt atggaaaact ggtgctggga gccggaggcg
     7981 ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc tgccgaaaga gttgctggat
     8041 aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta ttctgcgtca gctggagttc
     8101 ggcctgtttg atttccgcct tcatgccgag ttccgcccgg atcagggggc aaaaatcctc
     8161 gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc catctccgtc ctggggccgt
     8221 ttcccgcacg ctttcagcca tattttcgcc ggtggttatg cggcaggtta ctacagctac
     8281 ctgtgggctg acgtactggc ggcagatgct ttctcgcgct ttgaggaaga gggcattttc
     8341 aaccgtgaaa ccgggcagtc gttcctcgac aacattctga gccgtggcgg ttcagaagag
     8401 ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc agctggatgc gatgctggag
     8461 cattacggca ttaagggctg atcattcagt gaaaatctgc ttaattgatg aaacaggcgc
     8521 cggagacggc gccttatctg ttctggcggc ccgctggggg ctggagcacg atgaagacaa
     8581 cctgatggcg ctggtgttaa cgccggaaca tctggaattg cgcaagcgtg atgagccaaa
     8641 acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac gcaaattcgg
     8701 cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt aaaggcgatt atttgccgga
     8761 tgtggtggac gccaccgcag ggctggggcg cgatgccttt gtactggctt cagtcggctg
     8821 ccgcgtgcgg atgctggagc gtaatccagt ggttgccgcg ctactcgacg acggcctggc
     8881 gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag gagcggttgc agttaattca
     8941 cgcctccagc ctgacggcgc taaccgatat taccccacgc ccgcaggtgg tctatctcga
     9001 tccgatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc gtgtctttca
     9061 gtcgctggtg gggccggatc ttgatgccga tggattactg gagcctgcac gcttgctggc
     9121 gaccaaacgc gtggtggtaa agcgcccgga ctacgcgcca ccgctggcga atgtcgccac
     9181 gccaaacgcg gttgtgacta aagggcatcg ctttgatatc tatgcaggta cgccagtgta
     9241 aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat taatggcttt
     9301 ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa cagcatcact actgcaacgc
     9361 ccagcgtgac cagaccaatc ttaccaaaca cgttggtata gacgggcaac gtctcaagcg
     9421 gatcggtaat gttgtccggc accgcggtaa atgttgccac atagccgccc agcaagaacg
     9481 cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc tgcggcacca
     9541 gggcagcaat catcgccagg ccaagggcgc taataaacaa ttcacccagg ctctggaata
     9601 aatacaccag cacgataaac catggcgatg tcagcccttg ggcatccgca aaccacattc
     9661 ccgcagcagc agccgtcaaa aagcccagtg agcacataaa catgccgaga gtaaatttca
     9721 tcggcatcga gaggtctttg cctttgttac ccagatgcgt gtaaatgcct gccagtattg
     9781 ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg ttgatggaaa
     9841 aaccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgat gttggcatct
     9901 gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg acaaacattt
     9961 tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg gtgacgacga
    10021 tggagagaac aatcagcacc agattggcga cttctacgtt gtgcatcagc catgcacata
    10081 cgaagatcat caccacgctg ccaagtaata cgtacaacag tttgctgaag ctcatcggct
    10141 tgaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg taaaccagta
    10201 atgcgataat taaccccgcc ccgcacaggt tgtaggtgac tgaataaccg aatctatcag
    10261 cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac atatagaaca
    10321 gggtgaatgc gccatcaagc cgcggatctt tcggcggata gcacttcgaa agcaagctgg
    10381 ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg atgaaaatca
    10441 ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc acaagtgctc
    10501 caagaacaat ggtgcgtttg gtccccaaca ggtggtcgcc gacatagccg ccaatggaaa
    10561 tgaggccata gaccagtgca gcaaaagcac caaaagtgac aaaagcctgc tcttgcgaga
    10621 atccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag tagccgaatc
    10681 gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc agcatcccca
    10741 tgggtgttgt tgtattaata tttattaacc ttccaatacc atcctgaaaa gtatgaacgc
    10801 gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat tgatgcggtg
    10861 aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa tgatgggaaa
    10921 tatgatggtg ttaaattttg tattggcagg agattttgat tgcgtacgca gatcgtaggc
    10981 cagataaggg gtttacgctg catcgggtaa caaggcgcgc caaaaagcaa aacgcccgca
    11041 tatgcgggcg ttcaggaaca cgtcgtggag ggatgattat tcttcttcgt cgcgcagcgg
    11101 aacaatcagc atatcaacgt gaacggtgtt gatcagctga cgtgcggaag acatcagttt
    11161 gctccagaag tcctggtggt gaccacaaac caccagatcc atatcgtatt tcttgattgc
    11221 atcgaccaga acctggccca ggtcgccgct gccgctcagg gtttcagtga ttggatagcc
    11281 tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct tcagagatgc gtttctgcat
    11341 atcacccaga ttcacatcaa taagcccggt gtataggtca gagtagttta catctacgtg
    11401 gatcagagaa actttcgcat tgtaggggcg agccatagag actgctttct ctaccagaac
    11461 tttgctttcc ggggagagat cgactgcgat gagaatgtgt ttataagcca tagtgttact
    11521 ccttccataa agttgtcgat gactggccag ctagcgtttc ttgtgccgtt acgtcacccg
    11581 cgtcctgcga actacgtgcg cggggctaac ccagccgtaa agattcagtg ttaagaccat
    11641 ccttacctta tagcgacccg gatgatccgt caatccgcct tgcttacccg ttagataaac
    11701 aaattttcga catgaatata ttgatagtgg ttaaccttct ggaaaaaaaa caacctgatc
    11761 tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt ttctccagga
    11821 cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg aactccgcgg
    11881 gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc tttatgtgtc
    11941 gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt agtggtactg
    12001 cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt tacctcacat
    12061 ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg ttatcgcgat
    12121 catcacgatg atgagtttat tcgccgctgt gagcgggtgc gtcggcagtt tattctgact
    12181 agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg gcattaagca
    12241 cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa ctgacccgct cgttttggtg
    12301 cgtacgatta caggaactgc aaggagagcc agtacagccc gccggaaagt agtaccgcag
    12361 ccggaagggt aaacacccag gccatcagaa tactggttac ggttttacgc tgtaagccgc
    12421 cgccatctac caccatcgtc cccgcgacag aagaggagag tacgtgagtg gtggaaaccg
    12481 gcatcccggt ataactcgcc aggccgattg acactgccgc cgtcatctgg gcagacatcc
    12541 cctgagcgta ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt gccacacgac
    12601 gccagccaat catcgtaccg atacctaacg ccagcgcgac cgccatgatg atccacaccg
    12661 gcgcatactc gatggtgcta agcatgtcgg acttcagttt cttcaacagg cgctgatcgt
    12721 cagcactcac accaggcatc ttcaccactt tgtcgatagt gtcagaaacg cacagcataa
    12781 tgcggcgcat ctggctacgt tgatcaagcg acagcttgtc gtagctttcc acatcggtgg
    12841 tcaacatacc tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg cagtggaact
    12901 ccgcaggttg cgttgcgcca gcttccggag ccggtactaa ctgatcagca ccggtagcct
    12961 gtttgagcag cgcaggatgc tgctcaaagt aagcttcgac gttgttgatg gcatcacggg
    13021 tacgggtgat ttcgtagcca gtggcattca tgttcaccac gaagcctgct ggcgcgacgc
    13081 caatcaatac caacataacc agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa
    13141 acgccacgcc gatagcggaa aggatcagcg cgatacgcgt ccagaacggc ggctttttct
    13201 tgccgtcttt cttttcacgc tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc
    13261 cgctccagta gcgacgcagc aagaaaatca gaccgccagc aaacaccagg ccgacaatag
    13321 gggaaacgat cagagaaccg aaaatactta atactttcgg gatattgagt gcatccacca
    13381 ctgacgtccc ggtcatcaac gcattggtta aaccaatccc gatgatcgcg ccaatcagcg
    13441 tatgagagct ggatgcaggt aaaccaaagt accaggtacc caggttccag ataatcgccg
    13501 ccagcaacat agagaacacc atggcaaggc catgagacga tcccatatta agcagcagat
    13561 ccgtcggcag catatgcaca atggcatagg caacactcag accacccagc aaaacaccca
    13621 aaaagttgaa caccgccgcc ataaccacgg cgagctgaga acgcatcgcg cgggtataga
    13681 taacggttgc cacggcgttg gctgtgtcat ggaaaccatt gatggcttcg tagaacagca
    13741 caaaagccag tgcaagcaat aataacagcc cggtatgcaa atccaggcca gcaaacaaat
    13801 gtagcatagg acgttacgcc attttgagga catgaacgcg gcgcattatc agtgactttc
    13861 gcggcgcggg caaagtgaaa tatagacttt ttttgatttg cctcctgtat ggatttcact
    13921 caaaaaataa ttatcttata taattcaggc aaatacttcc ttttagtaat attgatgctg
    13981 gtgcgaccac tgaggaatct ttacaattca cgcccgtttt ttctaagagg agcgcaacgt
    14041 ggaaaggttt gatgccatta ttataggcgc tggtgcggcg ggtatgttct gttctgcgct
    14101 ggcaggtcag gcaggacgcc gggttctgct gatcgataat ggtaaaatac cagggcgcaa
    14161 aatccttatg tctggcggtg ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc
    14221 ttatctgagc cagaatccgc atttttgtaa gtctgcactc gcacgtttta cccagtggga
    14281 tttcattgat ctggtcaata aacacggtat cgcctggcac gagaaaacgt tagggcaact
    14341 cttctgcgat gactccgcgc agcagattgt cgacatgctg gtggatgagt gcgagaaggg
    14401 caatgtaacc ttcagattgc gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt
    14461 cacgcttgat ctgaacggca tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg
    14521 gctgtcaatg ccggggctgg gcgcatcgcc gtttggttat aagattgccg aacaatttgg
    14581 cctcaacgtg ctgccgaccc gcgcgggtct ggtgccattc actctgcata aaccgttgct
    14641 cgaagagtta caggtgctgg cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg
    14701 caccgttttc cgtgagaact tactcttcac ccaccgcggc ttatctggac cggcggtgtt
    14761 gcagatttca agctactggc aaccgggtga atttgtcagt atcaatctgc taccggatgt
    14821 ggacctcgaa accttcctga atgagcagcg taacgcacat ccgaatcaaa gcctgaaaaa
    14881 cacactggcg gttcatctac cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat
    14941 cccggatgtt tcgcaaaaac agctcaacgt gtgtgaccaa caggcactga ttagcacatt
    15001 gaccgactgg cgcgtacaac ccaacggcac tgaaggctat cgcactgccg aagtgacgct
    15061 cggcggcgtg gacaccaacg aactttcttc acggacgatg gaagcgcgca aagtgcctgg
    15121 gctgtacttc atcggcgaag tgatggacgt taccggctgg ctggggggct ataacttcca
    15181 gtgggcgtgg tcgagtgcat gggcttgtgc gcaggatttg gcgtcaattg tgaaataacc
    15241 atgacgtttt cccgtcagcc gtttgtatat gtcgttatta agtcattttg gtacggttct
    15301 gcatccgaca ttctggtgtt aactcgctgt tttgttagcg aattaacgct attgccgttg
    15361 tggttatttt atagtgtatt tattctcaga gaatatatat tactgaaata aaaaacacaa
    15421 agaataacca aaggaaatta tttgaaacgt aaaaggtata tatttttaat ctgtaaaatt
    15481 taaaatcacc ccctctggat tgaatcaact tgctctattt aagacgattg tttttacatt
    15541 ttcaccacct gtatcaggtg gtgttttttt attggcggca atgcctttat cttattttta
    15601 ttacgttgta aatatggcta ttcctaaatg aaatttgatt tgctcagttt atttgtgatg
    15661 tcaggggagt gcttgccatg agagtcgtga tattattatt catcattttt ataccgagtg
    15721 tggcggatgc tttttcattc gatagttcaa taattaatct caaatgtcct aggcgtggaa
    15781 ctatcgaaat aatattacac agatacgaac acacccagga aagctgggga ggggagaatt
    15841 ttgaaaccgg gagtgggtat agtcatgagg gcgcgttatt tatttttgac tttgccaatc
    15901 aggataaaat gatttacaat cgcgcggaga attcgtttgc tttttggtat gaatcttccc
    15961 ggaaattagt taactgtaac ttaataacgc ttaaaagcac atatcctgtt aacctgcctt
    16021 atgttcatgg ctgatgtgat gtgtgtcggg aactaacatc ttcatctttg tatatgacat
    16081 tacgataatg ttaagctggt ctttgtcata aatttaccct tagagatact actcccttct
    16141 gtcaatatta ttcaagccga acgttatact ggcttttgtc ggtcacggat atcttttttt
    16201 cagtgagaat tcaaagtgag ggtatataag aagtcgtatg agtaagtatc tttgttgtgg
    16261 tgggttatta ttttatgggc aaaatattaa gcagcgaaat cttttttttg cccagacgtt
    16321 ttttgtagaa aaaagaattt attagtccgg ctgaaaagca agaactgcta taactaatta
    16381 agcgtaacgg aacggtacaa aacatgccct tacaacaatt ttcccgatta gcgctcactc
    16441 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
    16501 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
    16561 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
    16621 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
    16681 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
    16741 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
    16801 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
    16861 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
    16921 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
    16981 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
    17041 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
    17101 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
    17161 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
    17221 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
    17281 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
    17341 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttactg cctctgacag
    17401 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
    17461 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
    17521 ctatagagct cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
    17581 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
    17641 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
    17701 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
    17761 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
    17821 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
    17881 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
    17941 cattgaaccg gtgagggccg ccccctttat tactcagggg gggctttcaa cgttctgaat
    18001 taagatgttt tatgactcac tgagcaggat gtgagttcca acgtaatctc cgctattttt
    18061 actgtttttc atttgctgcc tcatctatcc cttcgccttg cctctgagtt gcgttaaagg
    18121 ctgatgtact gcctttaacc cgttcaatac atagcctttt tgtcaatcat catcccgaag
    18181 agcgttgcac tgtgccttcc atagctgtgg ttgtcctctc aatctgtcag cccatttatg
    18241 gaaaatctta acgaagcaac aatgagatat gtaaaaaaag ggccgattta atcattcatt
    18301 aagacaggtc attttttctt actgtgaggg cggtaagctt ttctgtcggt aacaggttgg
    18361 gagatgctgg tattatgaga tttttctacc gtcctgcggc ggaatatgtg aaaaatagaa
    18421 atgattatta ttattgcatg gttattttta gtttgtgatg gtggagaata atcctattcc
    18481 cgtgaccttg ttttttacat tttatttaac tgcatggtat atgcattt
//
LOCUS       DADLSE010000078        18310 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLSE010000078 DADLSE010000000
VERSION     DADLSE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.078"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            264..491
                     /gene="feoA"
                     /locus_tag="JN692_002574"
     CDS             264..491
                     /gene="feoA"
                     /locus_tag="JN692_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7264855.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            508..2829
                     /gene="feoB"
                     /locus_tag="JN692_002575"
     CDS             508..2829
                     /gene="feoB"
                     /locus_tag="JN692_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7264856.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2829..3065
                     /gene="feoC"
                     /locus_tag="JN692_002576"
     CDS             2829..3065
                     /gene="feoC"
                     /locus_tag="JN692_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7264857.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3268..4146
                     /gene="rpnA"
                     /locus_tag="JN692_002577"
     CDS             3268..4146
                     /gene="rpnA"
                     /locus_tag="JN692_002577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7264858.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPEYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMLHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            4219..4488
                     /locus_tag="JN692_002578"
     CDS             4219..4488
                     /locus_tag="JN692_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7264859.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            4482..4847
                     /locus_tag="JN692_002579"
     CDS             4482..4847
                     /locus_tag="JN692_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7264860.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VTMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(4848..5636)
                     /gene="bioH"
                     /locus_tag="JN692_002580"
     CDS             complement(4848..5636)
                     /gene="bioH"
                     /locus_tag="JN692_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7264861.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            5674..6357
                     /gene="gntX"
                     /locus_tag="JN692_002581"
     CDS             5674..6357
                     /gene="gntX"
                     /locus_tag="JN692_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7264862.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRTTRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            6416..6991
                     /gene="nfuA"
                     /locus_tag="JN692_002582"
     CDS             6416..6991
                     /gene="nfuA"
                     /locus_tag="JN692_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7264863.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            7352..8668
                     /gene="gntT"
                     /locus_tag="JN692_002583"
     CDS             7352..8668
                     /gene="gntT"
                     /locus_tag="JN692_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7264864.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8713..10797)
                     /gene="malQ"
                     /locus_tag="JN692_002584"
     CDS             complement(8713..10797)
                     /gene="malQ"
                     /locus_tag="JN692_002584"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7264865.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10807..13200)
                     /gene="malP"
                     /locus_tag="JN692_002585"
     CDS             complement(10807..13200)
                     /gene="malP"
                     /locus_tag="JN692_002585"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7264866.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVLKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13812..16517
                     /gene="malT"
                     /locus_tag="JN692_002586"
     CDS             13812..16517
                     /gene="malT"
                     /locus_tag="JN692_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HAY7264867.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELVAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(16560..17576)
                     /gene="rtcA"
                     /locus_tag="JN692_002587"
     CDS             complement(16560..17576)
                     /gene="rtcA"
                     /locus_tag="JN692_002587"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HAY7264868.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(17580..>18310)
                     /locus_tag="JN692_002588"
     CDS             complement(17580..>18310)
                     /locus_tag="JN692_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7264869.1"
                     /translation="NHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQ
                     LETLPSRDLAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQ
                     PQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGL
                     GNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDID
                     AVMAAQSDLVEVIYTLRQVVCVKG"
BASE COUNT         4263 a         5008 c         4612 g         4427 t
ORIGIN      
        1 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
       61 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      121 gtattgctga tagaaaccat tctcattatc attgtgttgt tgattattta atctctcctt
      181 tgttggcaaa tcatctggtc tcatgtcgcg gtcaaacgcc ccatgaggta gttatccagt
      241 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      301 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctcta ggcatgttac
      361 ctggctcctc ttttgatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      421 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      481 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccattggctt aattggtaat
      541 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      601 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      661 gtcacgctgg tggacctgcc cggcacctat tctctgacca ccatctcatc gcagacctcg
      721 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      781 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctgcaact gctggaactc
      841 ggcattccct gcatcgtggc gctgaacatg ctcgacattg ccgagaagca aaatattcgt
      901 attgaaattg atgctctatc ggcacgtctt ggctgcccgg tgatcccgct ggtttcaacc
      961 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1021 gtggaactgg tgcattacgc acagccgcta ctcaacgaag cagattcact ggcaaaagtg
     1081 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1141 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1201 cgtctgcgta atgaaatgga tgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1261 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1321 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1381 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1441 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1501 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggtattaa caccgtgctg
     1561 ccactggtgc cgcagatcgg catgatgtac ctgttcctct ccttccttga ggactccggc
     1621 tatatggcgc gtgcagcgtt tgtgatggac cgcctgatgc aggcgctggg cttgccgggg
     1681 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggcgca
     1741 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1801 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1861 gccctggcgg ttttctcgct gtatatgctc ggtatcgtga tggcggtgct gactggcctg
     1921 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gctgccggtc
     1981 taccatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaagggcttc
     2041 gttctgcgtg ctggtaaagt gattatcatc gtcagcattt tcctgagcgc tttcaacagc
     2101 ttctcgttga gcgggaaaat cgtcgataac atcaacgact cggcgctggc ttccgtcagc
     2161 cgggtgataa cccctgtctt caagccgatt ggcgtacatg aagataactg gcaggcaacg
     2221 gttggcctgt ttacgggcgc aatggcaaaa gaagtggtgg tgggtacgct caacaccctc
     2281 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cggaattcaa cctcggtgaa
     2341 gagctgttca gtgcggtaga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2401 gtactgatga accccattga agctagcaaa ggcgacggtg aaatgggtac cggggcgatg
     2461 ggcgtgatgg atcagaaatt cggtagcgca gctgccgcat acagctacct gattttcgtc
     2521 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2581 tggatgggct tctccatcct gtgggggctg aatatcgctt actcactggc aacattgttc
     2641 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggta
     2701 atcctgttta acatcgtagt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2761 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2821 tgccattaat ggcttcactt attcaggtac gtgatttgct ggcgttacgg ggccgtatgg
     2881 aagcgaccca gataagccag acattgaaca ctccgcagcc aatgattaac gccatgctgc
     2941 aacagctgga aagcatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3001 ctggcagttg taaaagctgc ccggaaggca aagcctgtct gcgcgagtgg tgggcgctgc
     3061 gttaaccata ctccatcgcc tgttttgaaa agcccggtat gcgtttgcat ccgggctttt
     3121 ttgcgtgcgg ctttccataa aaatgcaatt cttgcagcgc aacgtaagtt cctctgaaag
     3181 cgtctcgcag ggatgaaaac tcgctaatgc acagatgtgg agtggcgcgt agagtcgcgg
     3241 cattcaaaca acaggtgaag gaacgccatg agtaaaaagc agagttccac cccacatgat
     3301 gcgctgttca aactcttttt acgccaaccg gagacggcac gcgattttct tgcgtttcat
     3361 ttaccggcac ccattcacgc gctctgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3421 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3481 caaggaccag aatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3541 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3601 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3661 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3721 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gctgcaccga
     3781 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgatct gatggggctg
     3841 gtagagcaaa tggcctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3901 ctgtttaatt acatactgca aactggcgac gctgtacgtt ttaacgattt tatcgacggc
     3961 gttgccgaac gttcaccgaa acacaaggag agtttaatga ctattgcgga aagattgcgg
     4021 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4081 cctcttgcag acatcatgcg atttaccggg ctgtcagaag aagagttggc tgcggcgagt
     4141 cagtagatac tctaccctgc ttaagtgcta ctttgtgcta tcttgcatgt aacacaaagt
     4201 agcacttagg gaactgatat gcagacagtg aaattacggc aacaaggagg agccatgatc
     4261 gttactatcc cacgagatct tgccatcgat cttggctggt caccgggcac agagcttacc
     4321 gtggagaaaa aaggcgatag cgttaatttg cgcgccacag agcacaaacc gcgtgggcgt
     4381 cttaccgtcg cgcagttgtt gagccaaatc gatgaaagcg aaattacgga gctaaatcag
     4441 tccacagaag gatgggctga agggaaaaaa ggtaatgaag catggtaaag gcacggacgc
     4501 cacatcgtgg tgagatctgg tattttaacc ctgatccggt tgccgggcat gaacttcagg
     4561 ggccacatta ttgcattgtg gtaacggaca aaaaactcaa caatgtttta aaagttacta
     4621 tgtgctgccc gatttcaaca ggggcaaatg cagcacgttc cacaggggtg acggtgaacg
     4681 tcctcccccg tgatacgcaa accggtaacc tgcatggcgt tgtactttgt caccagctaa
     4741 aagccgtcga tcttattgcc cgtggcgcta aatttcatac cgttgccgat gaaaaattga
     4801 ttagtgaagt tatcagtaaa ctggtgaatt taatcgaccc acaataatta gctctcactt
     4861 accaccaaca ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa
     4921 ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta
     4981 tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa
     5041 aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc
     5101 ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc
     5161 cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact
     5221 gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc
     5281 cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga
     5341 acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct
     5401 aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac
     5461 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
     5521 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
     5581 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
     5641 tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg
     5701 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ttcacgcacc
     5761 acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcccatctt
     5821 ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac ggttgccgac
     5881 tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc
     5941 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg
     6001 ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg
     6061 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccaatgg
     6121 gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct cagtgcccgg
     6181 ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat
     6241 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
     6301 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
     6361 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
     6421 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg
     6481 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc
     6541 ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac
     6601 cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac
     6661 cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc
     6721 agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca
     6781 gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat
     6841 tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg
     6901 gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga
     6961 acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat
     7021 gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcacagcat
     7081 tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt
     7141 aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca ttcacatggg
     7201 taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg
     7261 tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt
     7321 tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt
     7381 aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct
     7441 cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat
     7501 caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat
     7561 gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc
     7621 caaatttggt aaaaagcaca tccagtgggc ggtggtgtta accggtttta ccgttggttt
     7681 tgccctgttc tatgaagtgg gcttcgtgct gatgctgccg ctggtgttta ccatcgcggc
     7741 ttccgcgaat attccattgc tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac
     7801 ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt tcaatgccga
     7861 tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg
     7921 tccggtttac gctcgcgtgt tgaaaggtat cgataagcca atcccggaag gtctctacag
     7981 cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct
     8041 ggtgccggtt gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca
     8101 cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggcaacgc tgattgccgt
     8161 gctgattgcg atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga
     8221 cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg gtggtggcgg
     8281 tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca
     8341 cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc
     8401 gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc
     8461 aacgacgggt gttagcccag agctgatggt tattgcggtg ggttccggta gtgtgatttt
     8521 ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg
     8581 cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg
     8641 ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccggc
     8701 atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca aatccttcag
     8761 caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa gcttgcgtcg
     8821 ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca tatccagcca
     8881 gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc gctgcaaacc
     8941 acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag
     9001 acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc gatcctgata
     9061 cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca gcgttagatc
     9121 cccgctctcc cagtaaccac gcagcgttgg caggtcatgt gtcgccgcaa ccgccatcga
     9181 ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt cgaaatagag
     9241 cactttgtaa gagtacacac cgctgctgcg cagcttaccg acaatctcta ccggtacggt
     9301 accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg ccagaatcga
     9361 gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct cgccatacgg
     9421 tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg
     9481 catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga tgatatgcgg
     9541 gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc
     9601 aaccgatgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc caccttccgc
     9661 tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct ggcttatctc
     9721 ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc
     9781 atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct tatacatctc
     9841 tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagcgc
     9901 atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact ggcgaaacgc
     9961 ggccatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat
    10021 ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct gttgcagcgt
    10081 ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat ggaaatcttc
    10141 aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctgtatgg
    10201 gctggcgctc tccggatttg ccggatagag cgcatgaatc gggttcaggc caatgaacga
    10261 cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc
    10321 ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gccccccaca gcttttgttt
    10381 attcagcaaa gcctgcggtt cgtaacaacg tttcggggcg acaatcaccc ggcaatgcgc
    10441 gcgctggtcg tcctgggtga gtgttagcgt gtgataacct tccggcagct tcgtcggtag
    10501 attgaacgct ttgccccccg ttacatggcc tttgtactgc gttccttctt cggtggtcag
    10561 ctgccagcta tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata
    10621 aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc
    10681 gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat
    10741 gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga
    10801 gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga
    10861 gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg cctcctggtc
    10921 gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat ccgccatcac
    10981 cagatacgga tcgccgccct gtttgccgat actgtgcagc atctggtcga aggcatgctt
    11041 atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca gcaccttatc
    11101 tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca cttgttccac
    11161 ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt caacgttcgc
    11221 accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc
    11281 ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg ggatcagttt
    11341 ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat cgccaaccag
    11401 tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat tcttcgccag
    11461 gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc gatcagcctg
    11521 cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg
    11581 tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat
    11641 accggtacga actttcacaa actccgccag acggacttta ttcgcctgct tgatctcgcg
    11701 atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca gctgatcgag
    11761 atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg cagggttgca
    11821 ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt tgttcggcca
    11881 tagctggtga tattccggga acagatcttt caacaccaga tccgagtgca gcgccgcaac
    11941 accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttatcgtg
    12001 caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct ccaccagcgt
    12061 tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggtagta agcctttcac
    12121 cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa
    12181 agttttgctg gtgatggccc aggcgtcatc ccagctcatc tggtgctcat cgatcagcac
    12241 gcgcagcagt tctggaatcg cgatagttgg atgggtatcg ttcagctgaa taacttcgta
    12301 atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac
    12361 cgaacaggca cactggaagt attgctgcat caggcgcagc tttttaccgg cagtatggtt
    12421 gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccttgct gttcggcgcg
    12481 caggaaatca ccgtcgttaa atttagttag atcaaacgga tgcgcatgcg tcgcctgcca
    12541 cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg
    12601 accggtaatg gtaaactccg gctcccagcg tccgtctttc atcactttac cgccaatccc
    12661 tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc gatgccagtc
    12721 atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac catattgata
    12781 gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga ggaagcacgc
    12841 cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc
    12901 agtcaggttg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt
    12961 caacaggttg ttgcccgtca gacgaccaat caaaaactcc attgagatgt agttaacatg
    13021 tcgctgattc gccaccggtt tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc
    13081 actcactgcc agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg
    13141 ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat
    13201 aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat
    13261 gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga
    13321 aatgtgatct cgaccactta aagctagtgc aaaccacagg attagcagca aatcaatgcc
    13381 atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag
    13441 cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcacaagt aaaatatcca
    13501 gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat ggcactttca
    13561 tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg gaaaccagta
    13621 ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga attgtgacac
    13681 agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga
    13741 ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga
    13801 agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc gaccataccg
    13861 tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga
    13921 tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg gcaggcaaaa
    13981 acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct
    14041 atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt gagacgatgg
    14101 cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg
    14161 cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc actaatccag
    14221 tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc accctggtgg
    14281 tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt gaccaactgc
    14341 tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt
    14401 gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg gatttgcgat gacgtttccg
    14461 gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc cactcagccc
    14521 ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg
    14581 atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt
    14641 tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc
    14701 gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgagt
    14761 ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag tgggaactgg
    14821 tggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc
    14881 ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc
    14941 tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc
    15001 ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta ttgcaggcgt
    15061 ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc cgtgctgaac
    15121 atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct ctgcgcgccc
    15181 aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa ctggcactgg
    15241 aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag
    15301 tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa accgaacaga
    15361 tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaattcag caaagtgaaa
    15421 ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac gcaggaaaaa gcgttccagc
    15481 tgatcaacga gcagcatctg gaacagctgc caatgcatga gtttctggtg cgcatccgag
    15541 cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga
    15601 ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc
    15661 aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagttgaac cgtctggaaa
    15721 acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg
    15781 tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg cgtcatacgg
    15841 ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac attgcccgcg
    15901 cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa ctcaatgaaa
    15961 atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac
    16021 tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac gcattaaaac
    16081 tggcgaatcg caccggattt atcagtcatt ttgtcatcga aggcgaagcg atggcgcaac
    16141 aactgcgcca gctgattcaa cttaatacgc tgccggaact ggaacagcat cgcgcgcagc
    16201 gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt
    16261 tcgttgaacg tctgctaaat catccggaag tgccagaact aatccgcacc agcccgttga
    16321 cccaacgtga atggcaggtc ctggggctga tctactccgg ttacagcaat gaacaaattg
    16381 cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa
    16441 aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg ctgaagatga
    16501 tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac atctttacat
    16561 cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg caccggcaag
    16621 aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc gaccgtaaat
    16681 tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata ttcccccacc
    16741 gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc taccacctcg
    16801 gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc actttcgact
    16861 tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat attctgttca
    16921 tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg gcgcggcaca
    16981 ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag
    17041 gtgttaaacg atgccacggg cgagacttcc gttgccacca caccgcctcc ggcaggataa
    17101 aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag cagcggctcc
    17161 agcacccggc ggataaaatc cgcaggcggc gccgacgggt tatcggtgcc gccgctcact
    17221 tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg cagcaccagc
    17281 gtacaacttc cggcgctacc gatagcaaag cggtaatcgc cgccgcgcac ggtgccaggc
    17341 cggaagacca gacgctgcga tcccagctcc gcgccttcca ccgttgccct acaaatttcc
    17401 gcagccgctt ttaccgcggt cagatgctgg cgcagcagcc ccggtttcgc ccgtccggct
    17461 cgaatgccag tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc
    17521 aggatctgcc cgccgccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt
    17581 tatcctttta cgcacaccac ctgacgcagg gtgtagataa cttccaccag atcgctttgt
    17641 gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca
    17701 tctttacggc attccacatg cgcggtggca cgaatttggt cttccacgct gaacagtttt
    17761 ttcgctttag ttcggctcat tacccgcccg gcaccgtggc tgcacgaaca gaacgactct
    17821 tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg aataattcca
    17881 tattgaccag cacgcgcaga caccgcacct ttacgcgtca cgtagatctc ttcaccaaag
    17941 tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt
    18001 ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacatt ttccatcatc
    18061 gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag gtaatcatca
    18121 aagtattccg taccttccat aaagtacgcc agatcacgcg acggcaacgt ctcaagttgg
    18181 tcctgcatct ctttttgcgc cagatcgata aagtaagtcc cgatggcgtt accaattccg
    18241 cgtgaaccgg agtgcagcat aatccacacc tgctccgact catcaaggca gatttcaata
    18301 aagtggttac
//
LOCUS       DADLSE010000079        18204 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLSE010000079 DADLSE010000000
VERSION     DADLSE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.079"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            481..1137
                     /gene="pphA"
                     /locus_tag="JN692_002589"
     CDS             481..1137
                     /gene="pphA"
                     /locus_tag="JN692_002589"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HAY7264870.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIVLADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1138..1413)
                     /locus_tag="JN692_002590"
     CDS             complement(1138..1413)
                     /locus_tag="JN692_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7264871.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(1434..1670)
                     /locus_tag="JN692_002591"
     CDS             complement(1434..1670)
                     /locus_tag="JN692_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7264872.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN692_002592"
     CDS             complement(1788..3227)
                     /gene="rsmF"
                     /locus_tag="JN692_002592"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7264873.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN692_002593"
     CDS             complement(3307..5940)
                     /gene="yebT"
                     /locus_tag="JN692_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7264874.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDALIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN692_002594"
     CDS             complement(5909..7192)
                     /gene="yebS"
                     /locus_tag="JN692_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HAY7264875.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAE
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7322..7819
                     /locus_tag="JN692_002595"
     CDS             7322..7819
                     /locus_tag="JN692_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7264876.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7916..8614
                     /gene="proQ"
                     /locus_tag="JN692_002596"
     CDS             7916..8614
                     /gene="proQ"
                     /locus_tag="JN692_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7264877.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8634..10682
                     /gene="prc"
                     /locus_tag="JN692_002597"
     CDS             8634..10682
                     /gene="prc"
                     /locus_tag="JN692_002597"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7264878.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQTAPVK
                     "
     gene            10874..11755
                     /gene="htpX"
                     /locus_tag="JN692_002598"
     CDS             10874..11755
                     /gene="htpX"
                     /locus_tag="JN692_002598"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7264879.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11801..13174)
                     /locus_tag="JN692_002599"
     CDS             complement(11801..13174)
                     /locus_tag="JN692_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7264880.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            13351..14142
                     /gene="kdgR"
                     /locus_tag="JN692_002600"
     CDS             13351..14142
                     /gene="kdgR"
                     /locus_tag="JN692_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7264881.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14285..14524)
                     /locus_tag="JN692_002601"
     CDS             complement(14285..14524)
                     /locus_tag="JN692_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264882.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14683..14826
                     /gene="mgrB"
                     /locus_tag="JN692_002602"
     CDS             14683..14826
                     /gene="mgrB"
                     /locus_tag="JN692_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7264883.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14901..15188
                     /locus_tag="JN692_002603"
     CDS             14901..15188
                     /locus_tag="JN692_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264884.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15858..16001
                     /locus_tag="JN692_002604"
     CDS             15858..16001
                     /locus_tag="JN692_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7264885.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16014..16223
                     /gene="cspE"
                     /locus_tag="JN692_002605"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16014..16223
                     /gene="cspE"
                     /locus_tag="JN692_002605"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7264886.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16389..17198
                     /gene="rlmA"
                     /locus_tag="JN692_002606"
     CDS             16389..17198
                     /gene="rlmA"
                     /locus_tag="JN692_002606"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7264887.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPEPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN692_002607"
     CDS             complement(17195..17761)
                     /gene="mntP"
                     /locus_tag="JN692_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7264888.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPHRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4822 a         4347 c         4584 g         4451 t
ORIGIN      
        1 cccaaatcgt aaaggaaacc gtagcacgtc gtatgcaaga acgtgccacg gctggctgat
       61 ggatgttcga tagcgcgagt ttgaatgaaa atcagccgga gatgatttta cataattgct
      121 acggaattat tcaatacagg aattgcttgc gtctgcatgg attgacctga aatattcccg
      181 aaaatttctc taaaaaactc gaaaaaaatg gtaactggtt gaatgtatta atatgcaatg
      241 gtacgtgtca gggattaaaa gatgaacgta aatttattca acgcattaat tttaaagggt
      301 tttattgttt gttgacgaaa acaggaatcg tgttcggtct ctttttatct gttaaaagcc
      361 agaagcattt ccttcgctga ctttatagtc aaccataaca cacactctac tgtctgagtc
      421 cagcgttttt taacattctt gttaagatta tgtgatcttt agcgcgggag gaaaatattg
      481 atgaaacagc ctgcgcccgt ttatcagaga attgcgggtc atcaatggcg acatatctgg
      541 ctttctggcg atatacacgg ttgtcttgag cagttgcgcc gcaaattatg gcattgtcgt
      601 tttgatccgt ggcgagattt acttatctca gtgggagacg ttatcgatcg tgggccgcaa
      661 agtttacgtt gtttgcagtt actggaacaa cattgggttc gtgcggtaag aggcaatcat
      721 gaacagatgg cgatggatgc gctggcattc cagcagatgt ctttgtggtt gatgaatggc
      781 ggcgactggt ttattgtgct ggcagataat caacagaaac aagcgaaaac ggcgctggaa
      841 aaatgtcagc atttgccctt tattcttgaa gtacacagtc gcaccggcaa gcatgttatt
      901 gctcatgccg attatccaga tgatgtttat gaatggcaaa aggacgttga tttgcatcag
      961 gtcttgtgga gccgctcgcg attaggtgaa cgcccaaaag ggcagggaat tacaggtgct
     1021 gatcatttct ggtttggtca tacaccgttg cgacatcgcg tggatattgg caacctgcat
     1081 tatattgata ccggtgctgt ctttgggggc gaattgactc ttgtgcaact gcaataatta
     1141 aaaatcacca tactcctgtg ctggtcgcca gaaaccatct ataaaatcct caatcggaaa
     1201 acaaccgcca tggcggatcc gttgatcgct catagaataa agacactgct gttccgtgtt
     1261 gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa aaagtaccag
     1321 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
     1381 acgggggaat aaccaggaca aataacccgc catgccgacg ggttattttt ggatcaggca
     1441 agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag gacagagtgt
     1501 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
     1561 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
     1621 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
     1681 tccatttagc tgccgattgt ggtacgacca gtccgatcgc acaatttttc agcctagttg
     1741 aggccgccga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
     1801 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
     1861 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
     1921 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctccgcttc ctgcggtgtc
     1981 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
     2041 cgataacctt tgttatgcgt ttcggcaagt ttaatcccca agcgagaaaa tcggacttta
     2101 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
     2161 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
     2221 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     2281 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     2341 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     2401 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     2461 gcttcgtttt cttcccggtt taaggtacag gtcgagtaaa ccagcgtacc gccaggacgt
     2521 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     2581 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     2641 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     2701 tcaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     2761 tttacccgac tggcggaaaa ctcattggca aggattgcac cttcgttatt catccgcgcg
     2821 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ctgtggtgca
     2881 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     2941 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     3001 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     3061 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     3121 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     3181 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     3241 tggcaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac gccggagcag
     3301 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     3361 caacaggaag tgtttgcctt cctgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     3421 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     3481 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     3541 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     3601 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     3661 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     3721 agtaatggtg gcctcttgta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     3781 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     3841 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     3901 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     3961 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     4021 gtgaagcgta atctgcccgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     4081 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     4141 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     4201 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     4261 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     4321 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     4381 actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     4441 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     4501 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     4561 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     4621 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     4681 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     4741 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     4801 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     4861 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     4921 gcgggtattt tcacgaagca gggtaacgac gctgggatca acggtcattt ccccagtgac
     4981 tttaccacca ggatttaaat ccagtttagt cagctgtccg acttccagcc cctgatacat
     5041 taacggcgtc gagtcggcgg ttaatcctgc tccactcggc agttccagtt ttattattac
     5101 tccacgctgg ctgtgggcca gatcttcata cagaccaaag gtatcttccg cctcggcagg
     5161 tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg ccagactttc
     5221 cagtttcacc ttcgcgccac tgatactgac gttggcatca acgccggaaa cgttccagaa
     5281 acggctacct tttttcacca gatcggtaaa acgccgctcg atcagggcat caatcaccac
     5341 gccttgcttg ttgggattga tggcatagtc gtagactttt cccaccggga tcttgcggaa
     5401 atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag
     5461 atcgccattg tccagccgat atttcggttg ggtatcgagc gcgacaaagt gatcctgctc
     5521 tttaccttta cccggcatca tgccgatata gttcccaccg acgagggcgt ccaacccgga
     5581 gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt cgcgcagcgc
     5641 atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat cgtcgctgag
     5701 gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg tacggcccgg
     5761 aacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt cctgataact
     5821 gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc agaaaggtga
     5881 gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat
     5941 gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc aaaattacgg
     6001 cagcgccaaa ataaaactca gccggtccca tagtaaaagc gaggatctga tcgcgattaa
     6061 tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg
     6121 tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc
     6181 tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata ctggcgataa
     6241 acacgattcc cgcaaccgca atgttgctac ttgccagcga cataattccg gaaagaatcg
     6301 tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg ttggctggta
     6361 acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta tgacgacgac
     6421 gcaggcgtag cgggatatgg caacgcgggc agagaccacg ctgatcagga tagccggtaa
     6481 aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg cgctgcggat
     6541 aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca
     6601 ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa tcctgtacct
     6661 ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac tctttaagtc
     6721 gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca aaccataaat
     6781 atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag accattgatc
     6841 ccgttatcgc atcgccctgt ttggtcattt gccagatgcc ttgcatgacg ttggcgtcaa
     6901 tacgaatgcc taacagccag atatgcaaca gcggttcgcc ccaggcaaac ggcataagca
     6961 acagcatggt gaaggccatt gccgccaggc gcgttagcga ccagtcgcgc ccgtcgcgaa
     7021 tttttgcctg acagcgcgga caataggcac tttgatgaga atttatctcg ggcaggctaa
     7081 acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc tcgccgattg
     7141 ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc atgacgtgcc
     7201 aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt gagcattaac
     7261 gcatttaatg cttattttaa taggctggca gttccaggta agacaaccaa gtgattaaat
     7321 aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc tgatggcggg
     7381 agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg agcgtctcac
     7441 tgacgttaac tgggcaggtt tttatttgct tgaggacgat acactggtac tcggaccatt
     7501 tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg gcacagcggt
     7561 tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc atattgcctg
     7621 tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc agattattgg
     7681 tgttctcgac atcgatagta ccgtcttcgc tcgctttaca gacgaggacg agcaaggctt
     7741 acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca aaaaattctt
     7801 tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg tcattataat
     7861 gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa atttcatgga
     7921 aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg aacgttttcc
     7981 ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa attggtattt ttcaggattt
     8041 ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat ccgctttacg
     8101 tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa cgcgtgtcga
     8161 tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg ctcgcaagca
     8221 gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag cgaaaaaacg
     8281 cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg cgccgcgaac gcaagccacg
     8341 tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc aaaagccggt
     8401 agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc cggtttctga
     8461 catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa acgcgatgga
     8521 tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc cagctgaatt cgggtatgtc
     8581 tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca ggcatgaaca
     8641 tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag accttcgctg
     8701 tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg cagcatgcga
     8761 cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag ttcgacctcg
     8821 atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat tacagccaca
     8881 atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag ttaggcgatg
     8941 aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg caaaagcgcc
     9001 gttttgaacg ttaccagtac gctttgtcgg tactggaaaa gccgatggat ttcaccggca
     9061 acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag gctgagttga
     9121 acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg acaggaaaaa
     9181 cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt cgtcgtctgg
     9241 cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg cgtgaaatcg
     9301 atccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact gaaatgagtt
     9361 tgtcactgga aggtattggc gcagtgctgc aaatggatga tgactacacc gttatcaatt
     9421 cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt gacaaaattg
     9481 tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt cttgatgatg
     9541 tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt ttacctgctg
     9601 gtaaagggac caagacccgt actgtaacgt tgacccgtga acgtattcgt ctcgaagacc
     9661 gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg ctggatattc
     9721 cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa ctggaaaaac
     9781 agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg ttgaccgaag
     9841 cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc cgcgataaca
     9901 acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa ggcccgctgg
     9961 tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg gcaatgcagg
    10021 attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc gttcagcaat
    10081 accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca gcgctgggtt
    10141 ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg caacgtaaag
    10201 gcgtaacacc agacatcatc atgccgacgg gtaatgaaga aacggaaacg ggtgagaaat
    10261 tcgaagataa cgcgctgccg tgggatagca tcgatgccgc gacttatgtg aaatcaggag
    10321 atttaactgc ctttgaaccg gagctgctga aggaacataa tgcgcgtatc gcgaaagatc
    10381 ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgaag gacaagcgca
    10441 atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat gatgcgacgc
    10501 gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg aagaagctgg
    10561 atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg gtgaatatcg
    10621 cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaaaccgct cccgtcaagt
    10681 aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag atgccgtaaa
    10741 tcagatgcta caaaatgtaa agttgtgtct ttctggtgag ttacgcacta tccagacttg
    10801 aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt taaactgagg
    10861 taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg ccgtaatggt
    10921 cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc aggggctgat
    10981 gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga tgtccaaatg
    11041 gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg aaagggaacg
    11101 ttggctggtc aatactgtag caacccaggc tcgtcaggcg gggatcgcta tgccgcaagt
    11161 ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc gtgatgcctc
    11221 tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag ccgaggcggt
    11281 aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga cgctgattca
    11341 gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc ttgccgcggg
    11401 ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc tgatctactt
    11461 tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta tcaccatgtg
    11521 gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg ttggtcgcga
    11581 gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag aagcaaccag
    11641 catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt tcatgactca
    11701 cccgccgctg gataaacgta ttgaagctct gcgtacgggt gaatacctga agtaatatga
    11761 agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccctg gctcgtggct
    11821 gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc gcagccatca
    11881 gcgagacgtg cgtaccatta tcaccaaact gatttagcat cagcgccacc agcgccgcgc
    11941 cactactctg acccagtaga cgagccgttc ctaacatgcc actggcccca ccgctacgtt
    12001 cgcgcggagc ggaggtaata atggtgtggt tattgggtga ctggaataag ccaaatccag
    12061 cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg ggcagcagaa
    12121 ccagggaaaa aagccccgca gccatgatga acaaccctaa agcacccagc aatcctgcat
    12181 gtacgcgttc aatcaaatag cctgccagcg gagccatcac catcgttgct aacggccacg
    12241 gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc aggtaaaagg
    12301 gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg caaatagaaa
    12361 gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga agagaaagct
    12421 ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca gcaattaacg
    12481 tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg aaggttaacg
    12541 cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa ccattgggtg
    12601 gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg attaaaaata
    12661 accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg gcagcagaag
    12721 agacggcaac aataaacgag tttatgccca tccctctccc cagaaaacgt tgtggataga
    12781 tcaggcggat aagtgcggta ttaacgctca tcaacgctgc accgccgaaa ccttgtatga
    12841 cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc gcagaacagt gaagacaaca
    12901 gaaaaacgac cagaccgcat ttataaatac gtcgatagcc aaacatatcg cccagaaacg
    12961 aaaacgagag cagggagatg acaatggcga tttgataggc gttcactacc cagatggaac
    13021 tggctggtgt ggcatgaagg tccgtggcga ttgttggcag ggcgacgttg gcgattgcgc
    13081 cgtcaaggac agccatcgaa ataccaatca caatggttaa tatcgcaccg tatcgctggg
    13141 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    13201 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    13261 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    13321 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    13381 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    13441 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    13501 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    13561 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    13621 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    13681 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    13741 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    13801 ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    13861 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    13921 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    13981 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    14041 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    14101 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    14161 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    14221 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttatttatt
    14281 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    14341 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    14401 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    14461 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    14521 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    14581 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    14641 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    14701 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    14761 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    14821 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    14881 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    14941 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    15001 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    15061 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    15121 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    15181 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    15241 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    15301 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    15361 tttattagga tgtttacatc agatttgtga ttaagcgtgg tattatttat tagcgaaacg
    15421 tttctctctt gatttttttg ctcactcatc aatttttctt attttaaatt tacaatccct
    15481 ttggggattg acttctcttt agggtaattg atagccgtta actgactgtt ttatgagaaa
    15541 aagtgatata actttttatt cattgcatag cagaaaatgt gatattgcac gcactatgta
    15601 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaactacgcc
    15661 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    15721 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    15781 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taaaagattt cgggttaaac
    15841 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    15901 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    15961 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    16021 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    16081 ctgatggcag caaagatgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    16141 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    16201 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    16261 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    16321 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    16381 gagttgtcat gtcgttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    16441 gctatatctg tccccaacga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    16501 tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    16561 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    16621 tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    16681 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggc ctggatgtct
    16741 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactctt tgtgtcgctt
    16801 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    16861 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    16921 cgccggaacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    16981 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    17041 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttgcct
    17101 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    17161 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    17221 tctggacgcc gatgccgatc agcaccagcc cgccgagaat ttccgctttt ttcccaataa
    17281 ttgagccgat aaagcgacca accatcatcc ctaatgtcga cataatcaag gttgcacaac
    17341 caatggccaa tgcggtcgcg ataatgttga cctgaaggaa agcaagacca acacccacag
    17401 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgccgac
    17461 ggtgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    17521 cgaggaatat cagcagcaca aacgcaatcc agtgattcca ttcaaggaca aaccggctgg
    17581 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    17641 ggccggttcg caatgcttca gaaaatttcg gtttatggag gggggcacct ttaccgattg
    17701 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    17761 tgacaacgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    17821 catgaacaac aagccgcgat gggttttcgc gttcatgcag gcatagcgat tgatgtgcat
    17881 atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt
    17941 tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta
    18001 acatattttg agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa
    18061 cgattttcat ttatgtttat atatcaatga aacgttaaaa taaataacaa ataagggaag
    18121 agataatata gcctaagcta tgtctgatta ttaaaaatac actaaacata gctttggcta
    18181 aattcataag ttattgagtt gata
//
LOCUS       DADLSE010000080        17467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLSE010000080 DADLSE010000000
VERSION     DADLSE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.080"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..670
                     /locus_tag="JN692_002608"
     CDS             <1..670
                     /locus_tag="JN692_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7264889.1"
                     /translation="LEIRAAGSKFAIANQYRALLSDIFKTAIASGLAEEDPASATRPF
                     RTEVKRSRLLIDEYLLIRKIADVQNEWFGLCMDLALVTGQREGDLAAMRWEDIRDGRL
                     YVEQQKTGAKIRISLPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRI
                     ARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGRE
                     WKDI"
     gene            906..1724
                     /locus_tag="JN692_002609"
     CDS             906..1724
                     /locus_tag="JN692_002609"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HAY7264890.1"
                     /translation="MTTRVIALDLDGTLLTLKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSRHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1725..2783)
                     /gene="modC"
                     /locus_tag="JN692_002610"
     CDS             complement(1725..2783)
                     /gene="modC"
                     /locus_tag="JN692_002610"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7264891.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2786..3475)
                     /gene="modB"
                     /locus_tag="JN692_002611"
     CDS             complement(2786..3475)
                     /gene="modB"
                     /locus_tag="JN692_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7264892.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3475..4248)
                     /gene="modA"
                     /locus_tag="JN692_002612"
     CDS             complement(3475..4248)
                     /gene="modA"
                     /locus_tag="JN692_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7264893.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4415..4564)
                     /locus_tag="JN692_002613"
     CDS             complement(4415..4564)
                     /locus_tag="JN692_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7264894.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4693..5481
                     /gene="modE"
                     /locus_tag="JN692_002614"
     CDS             4693..5481
                     /gene="modE"
                     /locus_tag="JN692_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7264895.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5549..7021
                     /gene="modF"
                     /locus_tag="JN692_002615"
     CDS             5549..7021
                     /gene="modF"
                     /locus_tag="JN692_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7264896.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7282..8298
                     /gene="galE"
                     /locus_tag="JN692_002616"
     CDS             7282..8298
                     /gene="galE"
                     /locus_tag="JN692_002616"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7264897.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8308..9354
                     /gene="galT"
                     /locus_tag="JN692_002617"
     CDS             8308..9354
                     /gene="galT"
                     /locus_tag="JN692_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7264898.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9358..10506
                     /gene="galK"
                     /locus_tag="JN692_002618"
     CDS             9358..10506
                     /gene="galK"
                     /locus_tag="JN692_002618"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7264899.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10500..11540
                     /gene="galM"
                     /locus_tag="JN692_002619"
     CDS             10500..11540
                     /gene="galM"
                     /locus_tag="JN692_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7264900.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLSPGEE
                     YSSLTEYQFIAE"
     gene            11743..12495
                     /gene="gpmA"
                     /locus_tag="JN692_002620"
     CDS             11743..12495
                     /gene="gpmA"
                     /locus_tag="JN692_002620"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7264901.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12662..13714)
                     /gene="aroG"
                     /locus_tag="JN692_002621"
     CDS             complement(12662..13714)
                     /gene="aroG"
                     /locus_tag="JN692_002621"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7264902.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            14030..14410
                     /locus_tag="JN692_002622"
     CDS             14030..14410
                     /locus_tag="JN692_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264903.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14524..15459
                     /gene="zitB"
                     /locus_tag="JN692_002623"
     CDS             14524..15459
                     /gene="zitB"
                     /locus_tag="JN692_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7264904.1"
                     /translation="MAHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGSLA
                     LLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVITIL
                     IVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHVLG
                     DLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPVSL
                     DIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYLMD
                     HYQIEHATIQMEYQPCHGPDCHLNEGGSGHSHHHH"
     gene            complement(15456..16175)
                     /locus_tag="JN692_002624"
     CDS             complement(15456..16175)
                     /locus_tag="JN692_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7264905.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16213..17256)
                     /gene="nadA"
                     /locus_tag="JN692_002625"
     CDS             complement(16213..17256)
                     /gene="nadA"
                     /locus_tag="JN692_002625"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7264906.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFVAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4447 a         4551 c         4351 g         4118 t
ORIGIN      
        1 tcttgaaatc agagcagctg ggagtaaatt tgctattgct aaccagtaca gggcgctttt
       61 atcagacatt tttaaaacag ctatagcttc tggtcttgcc gaagaagatc cagcttctgc
      121 aaccaggcca ttcagaactg aagtgaagcg cagtcgactg ttaattgacg agtatctgtt
      181 aatcaggaaa atagcagacg tccagaacga atggttcggc ctttgcatgg atcttgcact
      241 ggtaacagga cagcgcgaag gagatctggc ggctatgaga tgggaggata taagagatgg
      301 caggctatac gtggaacaac aaaaaacagg ggcaaaaatt cgcatttctc tccccaccac
      361 gatttccaga ttgaatttaa cacttgccga tgtactggat aacttgaaaa aaataaacgg
      421 taaaaacgaa aagttactcg gagggaagac agccaggaca atcgcagcac agttcaggat
      481 tgcccgagac acatccggat tgaaatggga aggtgaccca ccgccatttc atgaaatccg
      541 aagtttatcc ggtcgcctgc actcagcaga aaaaggcagt gatttcactc aggcgctcct
      601 tggacatcgt tcatccagca tgacggataa ataccgtgac ggacgaggga gggaatggaa
      661 agacatttag aattaatttc gaatgatttc gaaatgccac ttgtaattaa ttgattataa
      721 aggattaatt cctaaagagc atttgcttca tgaatgctcc tttgcattta gcaactgact
      781 gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc agaattttgt
      841 ccgatagtgg tatcggtgta ccattcgcgc agacgaaaat tcttaacctt aatctggaat
      901 acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg accctgaaaa
      961 agaccttgct tccatcatcg atagaagccc tggcccgcgc tcgcgaagca ggctatcaat
     1021 taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag gcgctggcgc
     1081 tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat gcaaaaaccg
     1141 tgctggaagc ggacccaatg cccgttaata aagccctgca actcattgag atgctgaatg
     1201 aacaccacat tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg
     1261 ggcatgtcat tcgcacgtct aactgggcgc aaaccctgcc gccggaacag cgtccgactt
     1321 tcacacaagt cgcttctctg gctgaaacgg cgcaacaagt taacgccgta tggaagttcg
     1381 ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac
     1441 tgggactgga gtgtgaatgg tccaggcacg atcaggttga tattgcacac ggcggcaaca
     1501 gcaaaggtaa acgtttgacg aaatgggttg aggcgcaagg ttggtcgatg gaaaacgtcg
     1561 tggcattcgg cgataacttt aatgatatca gtatgctgga agccgctggt acaggcgtgg
     1621 cgatgggcaa cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca
     1681 ccaccgacag cattgcccag ttcatttata gccacctgat ttaatcaggc ggttatcgac
     1741 acacttttaa tttgcgcgta cagccacagg ccaggtttga tcgccagttc atccctggcc
     1801 cacgggctga tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacttgg
     1861 ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc
     1921 tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct
     1981 tgcagcggtt cgcccagctt attgacccac aaatgctgat cgcccagcgc cagcgcggtc
     2041 atcgcgtaat gcggatgatg ctcaagcacc gtcactttca gaatgctact ttgttgctct
     2101 ttcggcagcc acggattcat cacgctactg ccccacactt cctccagcgc gccaaaggct
     2161 ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc
     2221 gaatggctga catacaacat cggaatgttg atttcccgtg tcagccgttg cagataaggc
     2281 aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat
     2341 tccggtgctg tcagcaaagc ccgaccaatc gccacgcgct gtttttcgcc tccggacagg
     2401 ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac
     2461 tgatcgacca tacttttcga catgccgtag cgcagattgc cacgcacttt gtaatgcggg
     2521 aacagccgcg catcctgaaa aacatagcca acgcgacgct tttccggcgt caggcagata
     2581 cctttttcgg catcatttag tacccgccca ttgaggacaa tccgcccttt ttgcgggcgc
     2641 gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata
     2701 gcagtgatgc cattggcggg cagcgtttca ttaatggtca ggcaatggtt gcccaacgtc
     2761 tgggaaaaat tcagctccag catgattagc gccccgcccg ttcacggctg attctggcca
     2821 gccattctga aatcaacagg gagatcatcg ccagcgcaat agaaataatg cacagtctcg
     2881 ccgcgccact ttccccgccg ggggtctgga tcagggtata catggcagaa ggaatggttc
     2941 gcgtttcgct aggaatgttc gacacaaagg tgatggttgc accaaactca ccgagagaac
     3001 gagcaaaagc cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt aacgtgatag
     3061 taaagaaaac gcgccagcgc ccggcaccta gtgttcttgc ggcctgttcc agtttgacat
     3121 caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg
     3181 cagcgagaac cgcgccgcgc cagctaaagg cgaaggtaat accaaaccag tcatacagac
     3241 gttcaccgat aaatccgcgc cgtcccatcg aaactaataa taagtaaccg acgaccacgg
     3301 gcggtaacac cagcggtaga tgcagtacgc tgtcgagcag agctttgccc ggaaacgtgc
     3361 aacgcaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg
     3421 aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt
     3481 ggttgtaaat ccgtaacgtt taaagatttc cgctgcctgc ggtcccttca gataatcata
     3541 aaatgctttc actgtcgcat tgttatgccc ttccacaaca gcaaccggat attccacttt
     3601 tttatgtgaa tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc
     3661 agaaccgtag acaatgccca gaggcgcttc gttacgttcg accagcgcca gcgccccacg
     3721 aacatcttcc gctggggcca gtttcggaga gagcgtatcc catgcgccca gtttttgcag
     3781 tgcttctttt gcataaatgc cagcgggaac atgttccgga tcgccaaccg ccaggcgacc
     3841 gccattcagc agcgaagtcc agttagtttt gctgtcgatg gtgaaatctt tctgctcgct
     3901 ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctgacgcg tagcagtatc
     3961 gatcgctttt ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc
     4021 cgcaggggca cccgcttcaa tctgacgggc gagagtagat gacgaagcga aagaagaaac
     4081 cacatccacg cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag
     4141 tgatgcggcg gcgaacaccg tgattttccc ttcatctgcc agtgcattgc cagcaactgc
     4201 gaaagagagt gccgccccgg caaacaagtt caaccattta cgagccattg gtaacccctt
     4261 aatgtaacgt tgtgtaggcg acaatataac gactaactcg gaattttccc agcagttatt
     4321 gctaaccttt gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga agttgagaaa
     4381 aagaaaatgc ccgactaagc gggcattcag ggaatcaatg attttgtccg ggctggtctt
     4441 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     4501 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     4561 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagcac agaaaagcac
     4621 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc
     4681 caggacattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     4741 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     4801 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat
     4861 cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggaggcgca
     4921 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     4981 caaaaagcct ttgatgtgtt aagtgacgat gacgccctgc cgctgaacag cctgctggcc
     5041 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc
     5101 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga cggaaaaaca
     5161 cgcctgaaag tcgcaattac cgcacaaagc ggcgcacgtc tggggctgga tgaaggcaaa
     5221 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgca
     5281 caaaacgccg acaaccaatt accgggtatt attagtcata ttgagcgcgg cgcagagcag
     5341 tgcgaagtat taatggcgct acccgacggg caaacactgt gcgccacagt gccggtaaat
     5401 gaagcgactt ctcttcagca agggcagaat gtcacggcct actttaatgc cgacagcgtg
     5461 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     5521 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     5581 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     5641 ttgggcgttt gtcggttcga atggaagtgg aaagtcggcg ctggcccgcg cgctggcggg
     5701 ggaacttccg cttttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     5761 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcaa cggaataaca ccgatatgct
     5821 cggccctagc gaagatgaca ccggacgcac tacggctgag attattcagg atgaagtaaa
     5881 ggatgcaccg cgttgcatgc aactggcgca gcagttcggt attaccgccc tcctcgaccg
     5941 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat
     6001 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     6061 tcagcagctg gctgagcgac tcgcctcgtt acatcagtcc ggtattactt tggtactggt
     6121 gctcaatcgc ttcgatgaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     6181 tacgttagcg gaaactggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
     6241 tgcgcatagt gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg
     6301 tcacgcctta cccgccaacg aatcgcgcat tgtgctgaac aatggcgtgg tttcttataa
     6361 tgatcgcccc attcttaata accttagctg gcaggtgaat ccaggcgaac actggcaaat
     6421 tgtcgggcca aatggcgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     6481 gcaaggttac agcaacgatt tgacgctttt cggccgacgt cgcggcagca gcgaaaccat
     6541 ctgggacatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     6601 cagcactacc gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
     6661 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     6721 taaacacacg gctgacgctc cgttccatag tctttcctgg ggacagcagc gtctggcgct
     6781 gatcgtccgc gcactggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     6841 gcttgatccg ctcaatcgcc aacttatccg ccgttttgtt gatgtgctga ttagcgaagg
     6901 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
     6961 tcgccttgag ttcgtgccgg acggtggact ctatcgctat gtgctgacaa aaatatattg
     7021 agtcggtagt gctgaccttg ccggaggcgg cctaagcgcc ctctccggcc aacggttcga
     7081 cgcatgcagg catgaaaccg cttctttttt cagataaaaa gcgcaatcat tcataaaccc
     7141 tctgttttat aatcacttaa tcgcgcataa aaaacggcta aattcttgtg taaacgattc
     7201 cactaattta ttccatgtca cacttttcgc atctttgtta tgctatggtt atttcatacc
     7261 ataagcctaa tggagcgaat tatgagagtt ctggttaccg gtggtagcgg ttacattgga
     7321 agtcatacct gcgtgcaatt actgcaaaac ggtcatgatg tcatcattct tgataacctc
     7381 tgtaacagta agcgcagcgt actgcctgtt atcgagcgtt taggcggcaa acatccaacg
     7441 tttgttgaag gcgatattcg taacgaagcg ttgatgaccg agatcctgca cgatcacgct
     7501 atcgacaccg tgatccactt cgccgggctg aaagccgtcg ccgaatcggt acaaaaaccg
     7561 ctggaatatt acgacaacaa tgtcaacggc actctgcgcc tgattagcgc catgcgcgcc
     7621 gctaacgtca aaaactttat ttttagctcc tccgccaccg tttatggcga tcagccccaa
     7681 attccatacg ttgaaagctt cccgaccggc acaccgcaaa gcccttatgg caaaagcaaa
     7741 ttgatggtgg aacagatcct caccgacctg caaaaagccc agccggactg gagcattgcc
     7801 ctgctgcgct acttcaaccc ggttggcgcg catccgtcgg gcgatatggg cgaagatccg
     7861 caaggcattc cgaataacct gatgccatac atcgcccagg ttgctgtagg ccgtcgcgac
     7921 tcgctggcaa tttttggtaa cgattatccg accgaagatg gtactggcgt acgcgattac
     7981 atccacgtaa tggatctggc ggacggtcac gtcgtggcga tggaaaaact ggcgaacaag
     8041 ccaggcgtac acatctacaa cctcggtgct ggcgtaggca gcagcgtgct ggacgtggtt
     8101 aatgccttca gcaaagcctg cggcaaaccg gttaactatc attttgcacc gcgtcgcgaa
     8161 ggcgaccttc cggcctactg ggcggacgcc agcaaagccg accgtgaact gaactggcgc
     8221 gtaacgcgca cactcgatga aatggcgcag gacacctggc actggcagtc acgccatcca
     8281 cagggatatc ccgattaagg aacgaccatg acgcaattta atcccgttga tcatccacat
     8341 cgccgctaca acccgctcac cgggcaatgg attctggttt caccgcaccg cgctaagcgc
     8401 ccctggcagg gggcgcagga aacgccagcc aaacaggtgt tacctgcgca cgatccagat
     8461 tgcttcctct gcgcaggtaa tgtgcgggtg acaggcgata aaaaccccga ttacaccggg
     8521 acttacgttt tcactaatga ctttgcggcc ttgatgtctg acacgccaga tgcgccagaa
     8581 agcaacgatc ctttgatgcg ttgccagagc gcgcgcggta ccagccgggt gatctgcttt
     8641 tcaccggatc acagtaaaac gttgccagaa ctgagcgttg cggcattgac ggaaatcgtc
     8701 aaaacctggc aggagcaaac cgcagagctg ggaaaaacgt acccgtgggt gcaggtcttt
     8761 gaaaacaaag gtgcggcgat gggctgctct aacccgcatc cgcacggaca gatttgggca
     8821 aatagcttcc tgcctaacga agctgagcgc gaagaccgcc tgcaaaaaga atattttgcc
     8881 gaacagaaat caccaatgct ggtggattat gttcagcgcg agctggcaga cggtagccgt
     8941 accgttgtcg aaaccgaaca ctggttagcc gttgtgcctt actgggctgc ctggccgttc
     9001 gaaacgctac tgctgcccaa agcccacgtt ttgcggatca ccgatttgac cgacgcccag
     9061 cgcagcgatt tggcgctggc gttgaaaaag ctgaccagtc gttatgacaa cctcttccag
     9121 tgctccttcc cctactctat gggctggcac ggcgcaccat ttaatggcga agagaatcaa
     9181 cactggcagc tgcacgcgca cttttatccg cctctgctgc gctccgccac cgtacgtaaa
     9241 tttatggttg gttatgaaat gctggcagag acccagcgag acctgaccgc agaacaggca
     9301 gcagagcgtt tgcgcgcagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg
     9361 agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
     9421 accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
     9481 ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gctgtgcacc acgcgatgac
     9541 cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcaat
     9601 gcgcccattg tcgcgcatga aaactatcaa tgggcgaact acgttcgtgg cgtggtgaaa
     9661 catctgcaac tgcgtaacaa cagcttcggc ggtgtggaca tggtgatcag cggcaatgtg
     9721 ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg
     9781 cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca
     9841 gaaaaccagt ttgttggctg taactgcggg atcatggatc agctaatttc cgcactcggc
     9901 aagaaagatc atgccttgct gattgactgc cgctcactgg ggactaaagc agtttccatg
     9961 ccgaaaggtg tggctgtcgt catcatcaac agtaacttca aacgtaccct ggttggcagc
    10021 gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca gcagccagct
    10081 ctgcgcgatg tcaccattga agagttcaac gctgttgcac atgagctgga cccaatcgtg
    10141 gcgaaacgcg tgcgtcatat cctgactgaa aacgcccgca ccgttgaagc tgccagcgcg
    10201 ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggagtctca tgcctctatg
    10261 cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct
    10321 gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc
    10381 gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa
    10441 gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag
    10501 tgctgaacga aactcccgca ctggcacccg atggtcagcc gtaccgactg ttaactttgc
    10561 gtaacaacgc agggatggta gtcacgctga tggactgggg tgcgacttta ctttccgccc
    10621 gtattccgct ttccgatggc agcgtccgcg aggcgctgct cggctgtgcc agtccggaat
    10681 gctatcagga tcaggctgcg tttctggggg cctctattgg tcgttatgcc aaccgcatcg
    10741 ccaatagtcg ttataccttt gacggtgaaa ccgtgacgct ttcgccaagc cagggcgtta
    10801 accagctgca cggcggcccg gaagggttcg acaaacgtcg ctggcagatt gtgaaccaga
    10861 acgatcgtca ggtgctgttt gccctgagtt cagatgatgg cgatcagggc ttcccgggta
    10921 atctcggcgc gacggtgcaa tatcgtctga ccgacgataa ccgtatctcc attacttatc
    10981 gcgccacagt tgataaacct tgcccggtga atatgactaa tcacgtctat ttcaatcttg
    11041 acggcgagca gtctgacgtg cgcaatcaca agttgcagat tctggcggac gaatatcttc
    11101 cggttgatga aggcggcatt ccgcacgacg gcttgaaatc tgtcgccgga acgtcttttg
    11161 atttccgcag cgccaaaatc atcgccagtg agtttcttgc cgacgacgat cagcgcaaag
    11221 tgaaaggtta cgatcacgca ttcttgttac aggccaaagg cgatggcaag aaagtggcgg
    11281 cgcatgtctg gtcagcagat gaaaaattgc agctgaaggt ctacaccacc gctccggctc
    11341 ttcaattcta ctccggcaac ttcctcggcg gcacaccgtc gcggggaacc gaaccttacg
    11401 ccgactggca agggctggcg ttggaaagcg agtttctgcc ggacagcccg aaccaccctg
    11461 aatggccgca accggactgc ttcctgagtc ctggcgaaga gtattccagc ctgacggaat
    11521 atcagtttat tgctgagtaa tgttaagccc tcgtcatgag ggctttatct tcatattgtt
    11581 caaatcacca gcaaacaccg acatatttgc aactcaatat tcacaacaac cttacactgc
    11641 gccactattt tcgctatggt tatgcgtaag cattgctgtt gcttcgtcac ggcaatataa
    11701 tgagaattat tatcattaaa agatgatttg aggagtaagt atatggctgt aactaagctg
    11761 gttctggttc gtcatggcga aagtcagtgg aacaaagaaa accgtttcac cggttggtac
    11821 gacgtggatc tgtctgagaa aggcgtaagc gaagcaaaag cagcaggtaa gctgctgaaa
    11881 gaggaaggtt acagctttga ctttgcttac acttctgtgc tgaaacgcgc tatccatacc
    11941 ctgtggaatg tgctggacga actggatcag gcatggctgc ccgttgagaa atcctggaaa
    12001 ctgaacgaac gtcactacgg tgcgttgcag ggtctgaaca aagcggaaac tgctgaaaag
    12061 tatggcgacg agcaggtgaa acagtggcgt cgtggttttg cagtgactcc gccggaactg
    12121 actaaagatg atgagcgtta tccgggtcac gatccgcgtt acgcgaaact gagcgagaaa
    12181 gaattgccgc tgacggaaag cctggcgctg accattgacc gcgtgatccc ttactggaat
    12241 gaaaccattc tgccgcgtat gaagagcggt gagcgcgtaa tcatcgccgc acacggtaac
    12301 tccctgcgtg cgctggtgaa atatcttgat aacatgagcg aagaagagat tcttgagctg
    12361 aatatcccga ctggcgtgcc gctggtgtat gagttcgacg agaatttcaa accgctgaaa
    12421 cgctattatc tgggtaatgc tgacgagatc gcggcgaaag cagcagccgt tgcgaaccag
    12481 ggtaaagcga agtaaacgtc attcgtttaa aaaatgagaa agccgactgc aagtgagtcg
    12541 gcttttttgt ttgtcgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct
    12601 tatcaggcct gtggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac
    12661 cttacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca tcggtatctt
    12721 cccagccaat gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctatcga
    12781 ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataatg gccttttcgc
    12841 caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg
    12901 acgaattagc atggctgaaa tcgatcatca cctgagctgg cagacctgct ttgttcagcc
    12961 cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa
    13021 tgatatggca atcgccgtta ccactggtat tcacaatcgc cgaatgcccc catttagtta
    13081 cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg
    13141 taccgtctgt gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt
    13201 gcacctgcga ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat
    13261 actgaggagt gatcatatcg agaaactcac ccgccgctgg cagaccgctg tcgttaatat
    13321 caagcagcaa tttacgggct atacgcagac cgtcgttgat ctggaagctg ttatccatat
    13381 gcggatcgtt aatcaaccct ttccagccca ccgtggtacg cggcttttca aaatagacgc
    13441 gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat
    13501 actctttagc cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat
    13561 catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg
    13621 cattttcagt tgcggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt
    13681 ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat
    13741 cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta
    13801 cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg
    13861 agatctatcc tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc
    13921 tgctgcgttt cataaactgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg
    13981 cactgttgga agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac
    14041 aaaactggcc acattatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc
    14101 tgccgatagc ggagcgcaaa ctaataacgg ccaggcaaac gccgcagctg atgcgggcca
    14161 ggtagcccct gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa
    14221 taccggttct ggcggcacaa tgctgcattc ggatggttct tcaatgaaca atgacggaat
    14281 gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgatgtc cggatatcaa
    14341 taaaaaggta caaaccggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac
    14401 cacgcagtaa ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt
    14461 ttcacttcta aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc
    14521 gtcatggcgc actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg
    14581 ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt
    14641 tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg
    14701 cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc
    14761 ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt
    14821 accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgaggga
    14881 ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta
    14941 cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact gcatgtgctc
    15001 ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat ctggactggc
    15061 tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct gcgtagcgcg
    15121 tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat
    15181 atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat
    15241 gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca ggtgatcccg
    15301 ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag
    15361 attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg
    15421 aatgaagggg ggtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct
    15481 ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa ggtcaggatg
    15541 atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg
    15601 ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc
    15661 acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca
    15721 ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat
    15781 gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac
    15841 cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc gttctgactg
    15901 gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa cacctgtaat
    15961 agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt aacgttgatc
    16021 aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg
    16081 gcgatcgtgc ctaccgcttc aatccatgag agatcataac cgcccgcccc tattggtata
    16141 tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg
    16201 ccttattatt cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac
    16261 cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc cttcctgttc
    16321 taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat gcgcgcagct
    16381 gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat ctggcaccgc
    16441 ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgaggcaa
    16501 tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat
    16561 agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg
    16621 ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc agcataaaat
    16681 gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat
    16741 tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac
    16801 ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc
    16861 gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca
    16921 ctcagcctga agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc
    16981 tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg caacgaagcg
    17041 cgccatttcc agagaatcag aaatacagcc accagtttct tctgccagtt gttgaatttc
    17101 gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat
    17161 cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg
    17221 ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt
    17281 aacgttaaaa ccgacattag cgtaacattc gctgttatcg gtgtaatttg ttttatatgc
    17341 taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg
    17401 tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg
    17461 atgcctg
//
LOCUS       DADLSE010000081        17305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLSE010000081 DADLSE010000000
VERSION     DADLSE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.081"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN692_002626"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="JN692_002626"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7264907.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQVIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN692_002627"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="JN692_002627"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7264908.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRALRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN692_002628"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="JN692_002628"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7264909.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEASLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN692_002629"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="JN692_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7264910.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN692_002630"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="JN692_002630"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7264911.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN692_002631"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="JN692_002631"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7264912.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="JN692_002632"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="JN692_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7264913.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN692_002633"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="JN692_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7264914.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVL
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN692_002634"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="JN692_002634"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7264915.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN692_002635"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="JN692_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7264916.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /locus_tag="JN692_002636"
     CDS             complement(14846..15541)
                     /locus_tag="JN692_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7264917.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="JN692_002637"
     CDS             complement(15664..16701)
                     /locus_tag="JN692_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7264918.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..>17305
                     /locus_tag="JN692_002638"
     CDS             17034..>17305
                     /locus_tag="JN692_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="YhhY; regulated by the fur regulator; unknown
                     function; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7264919.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRW"
BASE COUNT         4248 a         4739 c         4310 g         4008 t
ORIGIN      
        1 cctttggccc cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat
       61 atgatcagcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgacc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gggaccacag tacaaaagca cgccgaagag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctga ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaggttgtg cactgtaaac accgacttcg caggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaagacc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gcttccagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ctggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg
     5041 atgcagttag acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacaac cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg
     5701 agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa
     5761 gagtaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccactaggag cgatatccca cggttcagca attaacttca
     6121 cctgcgagag cactgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga cttaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccgcct tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata
     7741 gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagcatc ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 acgataaatc atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat
     8281 cagcgtgttc cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg
     8641 ccaggaaccc aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat
     8881 gccgctctct ttacgcagtc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacgccg ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca agcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcacg cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gagcccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aagttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 agagtagttc agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa
    12361 gttaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccaag
    12481 caccaggaac gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgctg
    13261 catagcaaac gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtgggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatatggc accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgtg cgccgagata ggcaatgtgg cgatgcccgc gagcaataat
    14101 ggcggtagtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtaacat tgccttttac cacctggatc cagacgcggc
    15001 gttcagcggc aatttgatgc accgactgct catctttcag caacgcccaa cgatacagtt
    15061 ccatatcctg atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgcac ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcaca ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg
    16201 cggcagtcca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 gacaatctct cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc
    16321 aaagcgacga ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag
    16381 ctctttcgcc tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa
    16501 cttaacatcg ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgaaacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaa
//
LOCUS       DADLSE010000082        17236 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLSE010000082 DADLSE010000000
VERSION     DADLSE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.082"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            7..1737
                     /locus_tag="JN692_002639"
     CDS             7..1737
                     /locus_tag="JN692_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264920.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQLEKAMQLPSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVMPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYATNGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKLL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN692_002640"
     CDS             complement(1797..2288)
                     /gene="yajQ"
                     /locus_tag="JN692_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7264921.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2456..3367
                     /gene="panE"
                     /locus_tag="JN692_002641"
     CDS             2456..3367
                     /gene="panE"
                     /locus_tag="JN692_002641"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7264922.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3330..3920
                     /gene="yajL"
                     /locus_tag="JN692_002642"
     CDS             3330..3920
                     /gene="yajL"
                     /locus_tag="JN692_002642"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7264923.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN692_002643"
     CDS             complement(3974..5422)
                     /gene="thiI"
                     /locus_tag="JN692_002643"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7264924.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5628..5870
                     /gene="xseB"
                     /locus_tag="JN692_002644"
     CDS             5628..5870
                     /gene="xseB"
                     /locus_tag="JN692_002644"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7264925.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5870..6769
                     /gene="ispA"
                     /locus_tag="JN692_002645"
     CDS             5870..6769
                     /gene="ispA"
                     /locus_tag="JN692_002645"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7264926.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6794..8656
                     /gene="dxs"
                     /locus_tag="JN692_002646"
     CDS             6794..8656
                     /gene="dxs"
                     /locus_tag="JN692_002646"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7264927.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSCKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8711..9685
                     /gene="yajO"
                     /locus_tag="JN692_002647"
     CDS             8711..9685
                     /gene="yajO"
                     /locus_tag="JN692_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7264928.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN692_002648"
     CDS             complement(9739..10257)
                     /gene="pgpA"
                     /locus_tag="JN692_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7264929.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN692_002649"
     CDS             complement(10235..11212)
                     /gene="thiL"
                     /locus_tag="JN692_002649"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7264930.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKSGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN692_002650"
     CDS             complement(11290..11709)
                     /gene="nusB"
                     /locus_tag="JN692_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7264931.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN692_002651"
     CDS             complement(11729..12199)
                     /gene="ribE"
                     /locus_tag="JN692_002651"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7264932.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLE
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN692_002652"
     CDS             complement(12288..13391)
                     /gene="ribD"
                     /locus_tag="JN692_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7264933.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRYVGPDVCLHLVGA"
     gene            complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN692_002653"
     CDS             complement(13395..13844)
                     /gene="nrdR"
                     /locus_tag="JN692_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7264934.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13995..14534
                     /locus_tag="JN692_002654"
     CDS             13995..14534
                     /locus_tag="JN692_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7264935.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14833..15717
                     /locus_tag="JN692_002655"
     CDS             14833..15717
                     /locus_tag="JN692_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7264936.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15894..16241)
                     /locus_tag="JN692_002656"
     CDS             complement(15894..16241)
                     /locus_tag="JN692_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7264937.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            16366..17040
                     /locus_tag="JN692_002657"
     CDS             16366..17040
                     /locus_tag="JN692_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7264938.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYRRE
                     FKVRLAKQALQPGAVVARIAREHDINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIRE
                     MKGGIR"
     gene            17037..>17236
                     /gene="tnpB"
                     /locus_tag="JN692_002658"
     CDS             17037..>17236
                     /gene="tnpB"
                     /locus_tag="JN692_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7264939.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCL"
BASE COUNT         4421 a         4412 c         4276 g         4127 t
ORIGIN      
        1 tttgcgatga aagcgaaatc tactttgtta ttaatgattt tatctgtttt ttattctcat
       61 caggttttat ccagcgaacc cactcagccg gatgtttatt ctgtcgtcga gaaaaaacta
      121 aactctgctt tacctctgga agaaaatacc accttcagat cgcaggctga atggtttaat
      181 ttgcaatatg aattattagt atctggctac cctgaacgtg cttacaattt actgagcaaa
      241 cttgagacag aatcgaaaaa cgctaaatat tatctggatt taactgcctc cagagtcgct
      301 gaagaagaag caccagagac agccttaact tttttatcaa aaattgggtt aaataaaccg
      361 gctactctta catcctttga gagctatatc aatagctgga taaaaagcaa acaactcgaa
      421 aaggctatgc agcttcccag cctcgatgag aatgcactga attattattt accgactgtg
      481 ttagtcgcat atcagatcgt tcctgataag gcaataagta tttataattc tgtatatgga
      541 gataatgttg ttatgcccta tcagcaagtc accatgttac ttacagttgc ggagggttat
      601 caaacaaaag gcgatataaa aaacgccatt cattatgccg ataaggcgct gaatatgttt
      661 gataaagcta ttgctgtaga tagcagtcgt gaatcccatt atagtgatga gtatttgaaa
      721 ctcatgaata tctacgccac caatggcaac aaagaaaaag cggtggtttt atctcaacgt
      781 ctacacaaag ccattaccga atctcaatcg aattacttaa gtactctgga aggaatttta
      841 ttgttttata gaaataatga tatgcagcaa gactatcaaa acagcctttc aaattacata
      901 gtatttatcg ataaaatttt tgccttttcc ccatcaacaa gaagtgaatt aagcctcatt
      961 aatttattaa attcattaaa tgaggtggaa ttgatgagaa aacttctggc gtttctgatg
     1021 acagcaccag aatacgcttg ttatgatagt cagtattgtt atgaagacaa agtcagcgca
     1081 ttaaaaatcc tgtatcgtca tcatgacaat gagctaatgg acaaatattt cacgactctt
     1141 cttaaagaga tcagtaatca accattagaa gactgggata taataataag taatactggt
     1201 gaaaaattta gtgaagtggg tctaaccaaa tatgctcaac atctcgcatc tttcgctgaa
     1261 gaattttata taaaagagca aaaatcacat tctgaaagtg aaatgagagg actattttca
     1321 tcacttgccg aattatatag ttttggtaat gatactgcca gcgcaaaccg agtgttccat
     1381 cagcatgtcc ctgccttcaa tacagatcat atgattcaat attttataaa tgccaaagaa
     1441 tggaacaatg ccagggaatt attgataaaa gaggaaatgt tgggtatgca taatctgata
     1501 ttgttggaga atatttgtat gcaaaaaaat gcagaatgca tggcacacat tacctttacg
     1561 cttaataaat taacaacaca accagcaata acaaaaatag atagcgtggg taatgagcaa
     1621 ctttatcaga ttggcaaaat ctaccataag ttggaaatta aacctgagcc agagcaacag
     1681 gttttaattc agtcgcttta tgaccgggca tcaggaagcg cctctgcaac tcaataaatg
     1741 catggtcaaa agggccacca cggtggccct gacaatcagt agctatatcc agaaagttaa
     1801 tcgcggaagt ttttgaactg gaacggctga ccgagatcgc caccgcgcac catcgccatg
     1861 acagcctgca aatcatcacg agatttgcca gtcacgcgga tctcatcgcc ctgaatttgc
     1921 gcctgcactt tcagtttgct gtctttgatc atcttgacga tttttttctg agtggcgctc
     1981 tcaatgccct gtttcagttt cgcttccaca aaccaggttt taccgctatg aacgatattt
     2041 tccggtacat ccagtgaact gccttcaatg ccgcgcttca gcaacttggc acgcagaata
     2101 tccagcaact gattgacctg gaagtcggac tcgctcaaca ctttgatggt tttgctggcg
     2161 tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa aacgggactc cacttcgcga
     2221 ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa
     2281 gatggcatct tttcctctcc cttcattttt gatgcgaagc ataatacctg caaagttaaa
     2341 taaccaacag tcctgcacca ggacgtatcc cttaattgtg catagtcgca acagcatgca
     2401 caattaaggg atagccttat tgaacacccg gagtggttgc gggtgaggag gaacaatgaa
     2461 aattaccgta ttgggatgcg gtgccttagg gcaattatgg cttacagcac tttgtaaaca
     2521 gggtcatgaa gttcagggct ggctgcgcgt accgcaacct tattgtagtg tgaatctggt
     2581 tgagacagat ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg attttctcgc
     2641 caccagcgat ctgctcctgg tgacgctgaa agcatggcag gtttccgatg ccgtcaaaag
     2701 cctcgcatcc acactgcctg taactacgcc aatactgtta attcacaacg gcatgggcac
     2761 catcgaagaa ttgcaaaaca ttcagcagcc attactgatg ggcaccacca cccatgccgc
     2821 ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga atcacgcata tcggtccggc
     2881 acggcaacag gatggcgatt acagttatct ggcggatatt ttgcaaaccg tgttgcctga
     2941 cgttgcctgg cataacaata ttcgcgccga gctgtggcgc aagctggccg tcaactgcgt
     3001 gattaatcca ctgactgcca tctggaattg cccgaacggt gaattacgtc atcatccgca
     3061 agaaattatg caaatatgcg aagaagtcgc ggcagtgatc gaacgcgaag ggcatcatac
     3121 ttcagcggaa gatttgcgtg attacgtgat gcaggtgatt gatgccacag cggaaaatat
     3181 ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc gaaatcgact atatcaatgg
     3241 ttttctctta cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt
     3301 tgaaatggta aaaagaaagg agagtgaata tgagcgcatc ggcactggtt tgcctcgccc
     3361 ctggtagtga agagactgaa gccgtcacca cgatcgacct cttggttcgc ggcggtatca
     3421 aagtcaccac tgccagcgtc gacagcgatg gcaacctggc aattacctgc tcgcgcggcg
     3481 tgaagctgct ggcggatgcg ccgctggtcg aagtggctga tggcgaatat gacgtgatcg
     3541 tgctgcctgg tggcattaaa ggcacggagt gttttcgcga tagcactctg ctggttgaaa
     3601 ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca
     3661 ccgtgctggt gccgcacgat atctttccga ttggcaatat gaccggcttc ccgacgctga
     3721 aagacaaaat tcccgctgaa caatggcagg acaagcgcgt cgtctgggat gcacgggtaa
     3781 aattgctgac cagccaaggg ccgggtacag ctatcgactt tggtctgaaa attatcgacc
     3841 tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca actggtgatg gcggcaggga
     3901 tttataatta ttacgagtag tgtcggatgc ggcaaacgtc gcatctgacc agatgcgacg
     3961 taaaaactac aaattacggg cgatatacct tcacattgtt aaagccttgc tcgcgcagat
     4021 agagcgcctg cagacggctc atcaccccgc gctcacacca cagcagccag gttttgttct
     4081 ggtcgagatc gccaaatttg gtgctcagtt tatagaacgg cagagaaacc acatcaatcc
     4141 cttcgacttt cagtggctta tcttcctgtt catcgacaga acggatatcg agaatcacgt
     4201 cgttcgggcc gaagccgttg acggtttcca cttccaccac ttcctgctcg gtctgctggg
     4261 cgatttcgcg gatatcaacg ttattcgctt cctcaaccac tttatcgaga atactgaagt
     4321 cgaacttctc ttcttccgct tcaatcttcg atttaaccgc tttcaccgtc gggcttttgg
     4381 agatcacgcc acaatattcc ggcatcgtgc gagcaaagtc ttcggtgcca atctggcgag
     4441 ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg acgcaggatc agcgtgtcag
     4501 agacgttatc aatcaggcgc aggttggtca gcgtctgact ggacacctgg ccgagcgctt
     4561 cgccggtgac cagcgcctgt acgccgtaac gttcagccac tttagatgcg gcacgcacca
     4621 tcatacgttt gaggataacg cccatctgac cgtcgtcgat tttctcgaga atttccccga
     4681 cgaccggttc gaaattaatg gcgacaaaac gcacgcggtg ggagctgcca aagcggttcc
     4741 acagataatg cgccacctga cgcacgccaa tttcatgcgc tgcgccgccg aggttaaaga
     4801 agcagtaatg cacgcggcag ccgcgacgca tcaacatata actggaaaca ccggagtcga
     4861 aaccaccgga aatgagcgac agcacatctt cctgggtgcc aatcgggaaa ccaccaatac
     4921 cttcgtagcg acctttaatc agcaggaggc gatcgtcttc cacttccaga tgaacagtta
     4981 catccgggtt agtcagcttc acgcgcgcgg attcaatatg ctgatttaaa ccgccgccga
     5041 cgtaacgttc cacatcaatc gagctaaaat catgtttgcc acggcgcttc acgcgtacgc
     5101 agaaggtttt gccttccagc tgatcgcgat actgaaccaa cgctttctca aaaatatcgt
     5161 gcatgtcggt aaacggcacg tcttcgactt cgagaatatg gtggataccc ggaatacggg
     5221 tcagggcgtc gcgaatagtc agacgctggt tttcatcttt cgcgcgaact tcgatgttat
     5281 cccagtggcg gacgacagcg agcgtctcat catagtgctt taaaacgtta cgaatgttcc
     5341 cggtaaggat ttttataaag cgcaagcgca cagattggct tttgatggtg atttccggga
     5401 acaatttaat gataaacttc atggcggcaa tggttcgttg gcaagcctta agcgacttgt
     5461 atagggaaaa atacagcagc ccacacctgc ggctgcatcc aggcgcggaa gtataccact
     5521 aacatcgctt tgctgcgcac atcaccttac cattgcgcgt tatttgctat ttgccctgag
     5581 tccgttacca tgacggggcg aaaaatattg agagtcagac attcattatg ccgaagaaaa
     5641 atgaggcgcc cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc
     5701 tggaaagtgg cgacctgccg ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc
     5761 tggcacgtca ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa attctgctgt
     5821 ctgacaatga agacgcctct ctaacccctt ttacaccgga caatgagtaa tggactttcc
     5881 gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc
     5941 actgcccttt cagaacactc ccgtggtcga aaccatgcag tatggcgcat tattaggtgg
     6001 taagcgcctg cgacctttcc tggtttatgc caccggtcat atgtttggcg ttagcacaaa
     6061 cacgctggac gcacccgctg ctgccgtaga gtgtatccac gcttactcat taattcatga
     6121 tgatttaccg gcgatggatg atgacgatct gcgtcgcggt ttgccgacct gccatgtgaa
     6181 gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta caaacgctgg cgttctcgat
     6241 tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac agaatttcga tgatttctga
     6301 actggcgagc gccagcggta ttgccggaat gtgcggtggt caggcattag atttagacgc
     6361 ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt catcgtcata aaaccggcgc
     6421 attgattcgc gccgccgttc gccttggtgc attaagcgcc ggagataaag ggcgtcgtgc
     6481 tctgccagta ctcgacaagt atgcagagag catcggcctt gccttccagg ttcaggatga
     6541 catcctggat gtggtaggag atactgcaac gttgggaaaa cgccagggtg ccgaccagca
     6601 acttggtaaa agtacctacc ctgcacttct gggtcttgag caagcccgga agaaagcccg
     6661 ggatctgatc gacgatgccc gtcagtcgct gaaacaactg gctgaacagt cactcgatac
     6721 ctcggcactg gaagcgctag cggactacat catccagcgt aataaataaa caataagtat
     6781 taataggccc ctgatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc
     6841 cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg
     6901 ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac
     6961 ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccatttgacc aattgatttg
     7021 ggatgtggga catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg
     7081 caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga
     7141 cgtattaagc gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc
     7201 tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtg attggcgacg gcgcgattac
     7261 cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat attcgtcctg atatgctggt
     7321 ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtt ggcgcgctta acaaccatct
     7381 ggctcagctg ctttccggta agctttactc ttcactgcgc gaaggcggga aaaaagtttt
     7441 ctctggcgtg ccgcccatta aagagctgct caaacgtacc gaagaacata ttaaaggcat
     7501 ggtggtgcct ggcacgttgt ttgaagagct gggatttaac tacatcggcc cggtggacgg
     7561 tcacgatgtg ctggggctta tcaccacgct aaagaacatg cgcgacctga aaggcccgca
     7621 gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagacccgat
     7681 cactttccac gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg
     7741 cggtttgccg agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga
     7801 caacaagctg atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc
     7861 atgtaaattc ccggatcgtt acttcgacgt ggctattgcc gagcaacacg cagtgacctt
     7921 tgccgccggt ctggcgattg gtggatacaa acccattgtc gcgatctact ccaccttcct
     7981 gcaacgcgcc tatgatcagg tgctgcatga cgtggcaatt caaaagctac cggtcctgtt
     8041 cgccattgac cgcgcgggca ttgttggtgc tgacggtcaa actcaccagg gcgcttttga
     8101 tctctcttac ctgcgctgca taccagaaat ggtcattatg accccgagcg atgaaaacga
     8161 atgtcgccag atgctctata ccggctatca ctataacgat ggcccgtccg cggtgcgcta
     8221 cccgcgcggc aacgcggttg gcgtggaact gacgccgctg gaaaaactac caattggcaa
     8281 aggcattgtg aagcgtcgtg gcgagaaact ggtgatcctt aactttggta cgctgatgcc
     8341 agaagcggcc aaagtcgccg aatcgctgaa cgctacgctg gtcgatatgc gttttgtgaa
     8401 accacttgat gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt
     8461 agaagaaaac gccattatgg gcggcgcagg tagcggagtg aacgaagtgc tgatggccca
     8521 tcgtaaacca gtacccgtgc tgaacattgg cctgccggac ttctttattc cgcaaggaac
     8581 tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa
     8641 ggcctggctg gcataatccc tactccactc ctgctatgct taagaaatta ttcatggcag
     8701 gagtggcagc atgcaataca accccttagg aaaaaccgac cttcgcgttt cccgactttg
     8761 cctcggctgt atgacctttg gcgagccaga tcgcggtaat cacgcatgga cactgccgga
     8821 agagagcagc cgtcccataa ttaaacgcgc gctggaaggt ggcataaatt tctttgatac
     8881 cgccaatagc tattccgatg gtagcagcga agagatcgtt ggtcgcgcac tgcgggattt
     8941 cgcccgtcgt gaagacgtag tcgttgccac caaagtgttc catcgcgttg gtgatttacc
     9001 ggaaggatta tcccgtgcac aaattttgcg ctctatcaac gacagcctgc gacgtctcgg
     9061 catggattat gtcgatatcc tgcaaattca tcgctgggat tacaacacgc cgatcgaaga
     9121 gacgctggaa gccctgaacg acgtggtaaa agccgggaaa gcgcgttata tcggcgcgtc
     9181 atccatgcac gcttcgcagt ttgctcaggc gctggaacta caaaaacagc actgctgggc
     9241 gcagtttgtc agtatgcagg atcactataa tctgatttat cgcgaagaag agcgcgagat
     9301 gctgcctctg tgttatcagg agggcgtggc ggtgattccg tggagcccgc tggcgcgggg
     9361 gcgactgacg cgtccatggg gagaaactac cgcacgactg gtgtctgatg aggtggggaa
     9421 aaatctctat aaagaaagcg atgaaaatga cgcgcagatc gcagagcggt taacgggcgt
     9481 cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg gcctggttgt taagtaaacc
     9541 gggcattgcc gcaccgatta tcggaacttc gcgggaagaa cagcttgatg agctgctgaa
     9601 cgcggtggat atcactttga agccagaaca gattgccgag ctggaaacgc cgtataaacc
     9661 gcatgcggtc gtaggattta aataagagtc caggcctgat gagacgtgac aagcgtcaca
     9721 tcaggcatcg gtgcacaact acgacagaat acccagcggc cagtgatgac cgataaagta
     9781 caggatgcct gcggaaatca ccccggcgac aatatcgtcg atcatgatcc ccatgccgcc
     9841 atgtacattg cgatcaaacc agcggatcgg ccacggcttc cacatatcca gaatacggaa
     9901 aatcacaaac ccggcggcaa cccactgcca gtcattggtc ggcagcgcca tgagcgtgat
     9961 ccacatacca ataaattcgt cccagacaat gctgccgtga tcgtgcacgc ccatatcttt
    10021 cgccgtttga tgacaaagat agacgccgat acagatcccc aacatcacca ccagcgagta
    10081 gagctgccag ggtaaaaagg tcatcagata ccagaaaggg atcgccgcca gcgatcccat
    10141 cgtgccagga acgattgggc ttaatccact tccgaatccg acagcaagca gatgccacgg
    10201 attactcatc ttcaggcgac ttttcgcgac atctttatgg cgtggcaaaa tggtcatatc
    10261 ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa acaaagccct tcgatatcgg
    10321 cggtcatttg cccgatacag gtaaacggta cgccaaggtg tccgagagca acatccagcg
    10381 cgccacggtt cagctccggc accgtgaaac acaactcgta atcttcaccg ccagagagcg
    10441 cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag cgcatcagaa aacggcagca
    10501 atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac gatatgcccg agatcggaaa
    10561 tcagaccgtc agagagatcg atagctgaat ttgccagatc gcgcagtgcc tgcccctgta
    10621 aaatacgcgg cgatggacgg agatgacgtt tgatcaagta gtcagcatct ttagcatcgg
    10681 caacctgcaa acggttttgc aaaatcgcca gcccggcggc gctatcgccc ggtgtaccgg
    10741 tcacatagat ccagtcaccc gatttcgccc cagagcgcgt taaggctcgt cccatcggaa
    10801 caaagccgtg gatacccaac gtcattgata atggcccacg cgtggtatcg ccgccaatga
    10861 gttgcatatc gtaataattg agaagatcaa acaaactgtc gctgaaggac tcaagccacg
    10921 cttcgtctac gtccggtaag gttaatgcca gcgtcagcca ggccggatcg gcccccatcg
    10981 ctgccagatc gcttaggttc accgccagtg ctttataagc cagatcagca ggatcgatat
    11041 cagggaggaa atggttgccc gccaccagcg tatcagtgct gatcgccagg gtctgtttct
    11101 cggggatatt gagaagagcg caatcgtcgc cgatgcccag ttcgacatca agacgagaac
    11161 ttcttacacg gtcaaaataa cgggcaatca gggagaactc gccacatgcc atacgttatg
    11221 cctcagcagg taaaaaagaa aaggccggcg atcatggaac agtcttccgt gaatctaccg
    11281 gcctggatat tactttttgt tagggcgaat cacaggtgct gctttatcga gtacgccgtt
    11341 gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc agttcgatcg cttcgttaat
    11401 ggccactttg tatggcacat cactacgttt agacagttcg tacagcgcaa tgcgcagtac
    11461 tgctttttct acctggccca gttcttccag caggcgggac aggtagggct tcatcagtcc
    11521 gtcgaggtat gcggtgttag tcgccacccc ggccagcagc tcacggaagt acaggacgtc
    11581 aacgtctttt acatcctgtt cagccaggaa ctggtattca acatcagcga tgtcgttctg
    11641 ggacaactgc caggagtaga gcgcctggac ggcacactca cgagcgcggc gacgagcagc
    11701 aggtttcacg gatttcccct tactaatttc aggccttgat ggctttcaat acattaatca
    11761 tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc agctttggtg ccagcacgtt
    11821 cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa agcaaccgga atttcgctgt
    11881 cctgggcaac atgctccagg ccgttgcttg caccaccagc gacatattca aagtgggcag
    11941 tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc gtatttaccg gttttagcca
    12001 gtgcacccgc cgccagcggc agctcatagg caccaggcac ccaaacaacg gtaatgtttt
    12061 catcttttac ctgaccgata cgtttcagtg cgtcaattgc accttccagc aggctgtcat
    12121 tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg agcgtccggg gtagcaacgt
    12181 tagcttcaat aatgttcatt ttcttccttc gggttcgagt atggccccgc aggggggcgg
    12241 attttagcat aatatttcgt gcgctgcttc cctttcgagc cgggagatca tgcacccact
    12301 aaatgcaggc aaacatccgg gcctacataa cgtatctctt tgaatttaaa ttggggggcg
    12361 tcggctaatt tctcaagccc tggcagcgag cataatccac gggcgtcgct gcctaatagt
    12421 ttaggtgcga tatagacaat cagctcatcg actaaacccg cctgtagcaa tacgccagcg
    12481 agcgttggcc ccgcttccac ccagatgctg ttaatctgct gtttacccag ttgcatcatc
    12541 agtacaacca gatccagatg acctttatgc tctggaatca gcaaggtacg caccgtttcc
    12601 ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg tttcgccggg ctgctgcaca
    12661 atgcgatgtt ccggcgtcac gcgattttgg ctatcaatca caatacgtac tggctggcgg
    12721 agattttgtt gcgggtagag cgcctgagtt tgttcatcca gttcagacca acgcactgtt
    12781 aaggctggat catccgccag caccgtggcg ctgctggtta aaatggcatg actttgtgcg
    12841 cgctgtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga tccactggct ttcgccgctc
    12901 gccatcgccg tgcggccatc aagcgatgcg ccaagtttta actgaatata aggaaagccg
    12961 gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg cttcactcat catcagcccg
    13021 tggctgacgt caatgccagc ctgttgcaga cggtaaagtc cacgcccagc gacctgcggg
    13081 ttaggatctt gcatcgctgc gaccacgcgc gctacgccag cggcgatgag tgcgtcacag
    13141 cacggtggcg tacggccatg atggctacag ggttcgagtg tcacatacgc cgttgcgcct
    13201 ttggcttttt cacccgccat acgcaacgcg tgtacttcgg catgtggttc acccgctcgt
    13261 tggtggtaac cttcaccgac aatttcgcca tctttgacaa tgacgcaccc gacattcggg
    13321 ttggggtggg tggtaaaacg tcctcgttgc gccagcttta gcgcccgcgc catgtaatac
    13381 tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt cgccaaattc tttgatatct
    13441 tcgaaactgc ggtagacaga ggcaaaacgg atataggcga ctttatcgag ctttttcaat
    13501 tgctccatca ccagattgcc aatcatcttg ctcggtactt cgcgctcacc ggtggcgcgc
    13561 agctgcgatt taatatgatt gattgccatt tcaacgtaat cggaactcac cggacgtttt
    13621 tccagcgccc gcagcattcc gctacgcaat ttctcttcat taaacggttc acgcacgtcg
    13681 ttgcttttta caacacgcgg cataaccagc tccgccactt caaaggtggt gaaacgttca
    13741 ttacacacca gacactgccg acggcggcgt acggatgaac cctcgcccac gagacgagag
    13801 tcaattacct tagtgtccac ggcgaaacag aatgggcaat gcatacggcg tcctgaccag
    13861 gtggttaacg gaaatctatt ttaccctgaa ctgacgatac aacaaaggcg caacgctttt
    13921 gagataaagg atcgatgctt tcgctgcttt taccctctac cattaggcta attgcgacga
    13981 gaaagggacg acctatgaat actaacgttt ttcgactgct cctgctggga agcctgttca
    14041 gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat gaaacacagc gtcagcacgc
    14101 tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa aatcactcag caaaacaggc
    14161 tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg agcgaaaaca ccggcaagac
    14221 tggaaagcca gatcggtact ttacgtatgt cgctggtgaa tattacgcct gatacagatg
    14281 gcaccaccct tacactacgt attcagggtg agtcgaatga tcccttgccc gcattcagcg
    14341 gaactgttga atatgggcaa attcagggaa caatagacaa ctttcaggaa atcaatgtgc
    14401 agaatcaatt gattaatgcc cctgccagcg tcctcgcccc cagcgatgtt gatattccgt
    14461 tacaattaaa ggggatttct gtggatcagt taggctttgt acgtatccac gacattcaac
    14521 ctgttatgca gtaaacgtat ttcgggacga ttttgtgcgt cccgcaacat cttttcccgt
    14581 cattttgtta ctctgcttac atcacctgga ttgatagtaa aagtttgcaa caagggcgaa
    14641 agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac gcaatcgatt acgtaaatga
    14701 tagaactgtg aaacgaaaca tatttttgtg agcaatgatt tttataatag gctcctctgt
    14761 atacgaaata tttagaaacg caatttgcgc ctttttcact cccgcaaggg attttcaaac
    14821 agtggcatac atatgaaaaa aacattactg gcagccggtg cggtactggc gctctcttcg
    14881 tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt atctttccga ctggtggcac
    14941 cagagcgtta acgttgtcgg aagctatcac acccgtttcg gaccgcagat ccgcaacgat
    15001 acctaccttg agtacgaagc attcgctaaa aaagactggt tcgacttcta tggttatgcg
    15061 gatgcgccgg tattcttcgg cggtaactcc gatgctaaag gtatctggaa ccacggttct
    15121 ccgctgttta tggaaatcga accacgtttc tccatcgaca agctgaccaa tactgacctt
    15181 agcttcggtc cgttcaaaga gtggtacttc gcgaacaact acatttacga catgggtcgt
    15241 aataaagatg gtcgccagag cacctggtac atgggtctgg gtaccgatat cgacactggc
    15301 ctgccgatga gcctgtccat gaacgtctat gcgaaatacc agtggcagaa ctatggcgca
    15361 gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta aatactttgt gccgattacc
    15421 gatctgtggg gcggtcagct gagctacatc ggcttcacca acttcgactg gggttccgat
    15481 ttaggggatg acagcggtaa cgcaatcaac ggtattaaga cccgtactaa taactctatc
    15541 gcttccagcc atattctggc tctgaactac gatcactggc actactctgt cgtagctcgt
    15601 tactggcacg acggtggtca gtggaacgac gatgcagaac tgaacttcgg caacggcaac
    15661 ttcaacgttc gctctaccgg ctggggtggt tacctggtag taggttacaa cttctgatta
    15721 tgaaaatgcc gggatttatt cccggcattt ctgattgtta acaacgtgtg ctttgttcat
    15781 gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc acgtaaattc aatatattgc
    15841 tgaaataacg taggcctgat aagcgtagcg catcaggcaa ttttacgttt taatcacttc
    15901 ttcttgttca tcatcgcttt caggtcagcg aatgggttgt acttcgcttc accgacatct
    15961 ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc ggtatatttc
    16021 gaatgctcat gatcgtggca atagagacac aacaattccc agttactgcc atcttccggg
    16081 ttattggtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt agaataaaca
    16141 aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt ttcgcgatag
    16201 ccgctttcta accgtgcata gttttttggg atgatagcca tgaaaagtta cctggatgaa
    16261 atgtagggtt acgcgcagtt tccgtaagcg ccccatcagc gacgtcttgt gaaaattgtc
    16321 ctgtctggca acaatcgcgc ccatctatat tgatggacac gaacgatgaa ttcccagaca
    16381 acaaaagata ttccctgctt ccgttcttat ttgcctgatg ccctgcgttt aagatttgaa
    16441 gataaactga ccatccgggc catcgctcag cgtctgggtc tcagtcattc cacaatacat
    16501 acgctttttc agcgatttct tgcatccggt atcgcatggc cattgcccga ttcagtttca
    16561 ttcgctcaac ttgacgccat cctttatgcc aacagaaaga aggaattaac agagcctcaa
    16621 atcagagaag gctcatggcg aaaagaacgg cgaaccagct atcgccgtga atttaaggtc
    16681 cgtctggcta agcaggcgtt acagcctggg gctgttgttg cccggatcgc cagagaacac
    16741 gatatcaatg ataacctgct gtttaaatgg aaaagccagt acgaggacgg cttactgagc
    16801 gatgatgaca tacaggaatg catgcctgtc ccggtggcac tgactgatac gccggagccg
    16861 accagaccag ttacaaatcc cttctggcgt aacaagcatg atgagcgccc tgagggggct
    16921 cccggaaacg tcccacggtg cgagctgcat cttaaatcag gtgtggtaaa actgtttgac
    16981 cctctcactc cggaactgtt acgggcgcta atccgcgaaa tgaaaggggg tatccgatga
    17041 taacgctgcc gaccggtacc aaaatctgga tcatcgctgg catcacagat atgcgttgtg
    17101 gcttcaatgg cctggcttcg aaggtgcaga acacgctgaa agatgacccg ttctccgggc
    17161 atatcttcgt cttccggggc cgcagtggca aaatggtgaa aatactgtgg gccgatcgtg
    17221 acgggttatg cctgtt
//
LOCUS       DADLSE010000083        16949 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLSE010000083 DADLSE010000000
VERSION     DADLSE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.083"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(317..1513)
                     /gene="hemY"
                     /locus_tag="JN692_002659"
     CDS             complement(317..1513)
                     /gene="hemY"
                     /locus_tag="JN692_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7264940.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1516..2739)
                     /gene="hemX"
                     /locus_tag="JN692_002660"
     CDS             complement(1516..2739)
                     /gene="hemX"
                     /locus_tag="JN692_002660"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7264941.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(2761..3501)
                     /gene="hemD"
                     /locus_tag="JN692_002661"
     CDS             complement(2761..3501)
                     /gene="hemD"
                     /locus_tag="JN692_002661"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7264942.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3498..4439)
                     /gene="hemC"
                     /locus_tag="JN692_002662"
     CDS             complement(3498..4439)
                     /gene="hemC"
                     /locus_tag="JN692_002662"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7264943.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVVLLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4826..7372
                     /gene="cyaA"
                     /locus_tag="JN692_002663"
     CDS             4826..7372
                     /gene="cyaA"
                     /locus_tag="JN692_002663"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7264944.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7412..7732)
                     /gene="cyaY"
                     /locus_tag="JN692_002664"
     CDS             complement(7412..7732)
                     /gene="cyaY"
                     /locus_tag="JN692_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7264945.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7780..9093)
                     /locus_tag="JN692_002665"
     CDS             complement(7780..9093)
                     /locus_tag="JN692_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7264946.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYPNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9090..10555)
                     /locus_tag="JN692_002666"
                     /pseudo
     CDS             complement(9090..10555)
                     /locus_tag="JN692_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10744..10947
                     /locus_tag="JN692_002667"
     CDS             10744..10947
                     /locus_tag="JN692_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7264947.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            10984..11808
                     /gene="dapF"
                     /locus_tag="JN692_002668"
     CDS             10984..11808
                     /gene="dapF"
                     /locus_tag="JN692_002668"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7264948.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11805..12512
                     /locus_tag="JN692_002669"
     CDS             11805..12512
                     /locus_tag="JN692_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7264949.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLVRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12509..13405
                     /gene="xerC"
                     /locus_tag="JN692_002670"
     CDS             12509..13405
                     /gene="xerC"
                     /locus_tag="JN692_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7264950.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13405..14121
                     /gene="yigB"
                     /locus_tag="JN692_002671"
     CDS             13405..14121
                     /gene="yigB"
                     /locus_tag="JN692_002671"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7264951.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14205..16367
                     /gene="uvrD"
                     /locus_tag="JN692_002672"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14205..16367
                     /gene="uvrD"
                     /locus_tag="JN692_002672"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7264952.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16514..16945)
                     /locus_tag="JN692_002673"
     CDS             complement(16514..16945)
                     /locus_tag="JN692_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264953.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         3895 a         4418 c         4522 g         4114 t
ORIGIN      
        1 aattatctca taacaagtgt taagggatgt tatttcccga ttctctgtgg cataataaac
       61 gagtagatgc tcattccatc tcttatgttc gccttagtgc ctcataaact ccggaatgac
      121 gcagagccgt ttacggtgct tatcgtccac tgacagatgt cgcttatgcc tcatcagaca
      181 ccatggacac aacgttgagt gaagcaccca cttgttgtca tacagacctg ttttaacgcc
      241 tgctccgtaa taagagcagg cgttttttta tgcatcagaa aggtcccgga ggtgctttcc
      301 tccgggtgag aaggaactac tgtggcgggt tattctgcaa cgttaacatc aaaccgtcgc
      361 gacgcatcgc tgcagcttct tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg
      421 cgtaatcgta ggcgtccgga cgttgtttca gcgccgcgcg gaaggcgagc gatgcttcct
      481 gccattcacc gtgcttcatc agtgactgac ccaatgtgct ccacaacagc gggcgatcac
      541 cgacgttttt gatttgctgg cgtagcactt tttccagctg ttccgggtta ttggttttca
      601 gacgcgggat cggcagcagt aggcgatcgt catactggcg tttcaggccg tcgatgataa
      661 tttgctgggc agtatcatga tcgtcacatt caataagatg ttccgccatt gccacctgca
      721 acgctacctg atgacgcgtt ttccggcttt ggtttttcca ccagttacgc aaaccttcac
      781 taccgttatc ggcacgcgcc tgatccatca ggccaatcca tgcctgttgt tccagcattg
      841 cacgatgttc ttcatcacca acatgggctt tcgccattga tgggatgata tccagcagag
      901 aactccatgc ccctgtgcgg atatacgcct gttccgccag acgtaatact tcaggatggc
      961 gtggcgtaac ttccagcagc ttatccacgc cgtggcgtgc agcatggttt tcattacggg
     1021 ccagttgcag acgtacacgg gtgatttcta ccggaatggt gtcattgccg gccagctccg
     1081 ctgcgcgttc cagatgttgg ttggcgcgtg cttcatcacc acgttgttgc gcggcttcgg
     1141 cagccagtag atagttcacc accggttgtt cagcgtgatc ggcatttttc gccatcagct
     1201 tttcaacttg ctgataatca ccttccgcca gtttcagcag cgcctgttcg gtctgcttac
     1261 gcgcacggcg acgcttgcgt ccgacaaacc atccacgggt gtgcgcgcca gtgcggaaga
     1321 tccgccgcag tagccactca atggcaaaca gcactaccat cgccagaatc aatatgatcg
     1381 ccaggcccgt gacgctggtt tcgatattgt agttgtcggt ctggatcagc acataaccct
     1441 gatgaccggc aatcatcggg ccaaccacta tccccgcaat cagcaaaaca aagagtaata
     1501 acacttttag catggttatt ctccttgcgg cgcggctgcc ggagtatcac cttgcggtgc
     1561 aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc
     1621 cgttgtcccc gctgccggtt gtgccagcag gttacgcacg cgagtctgca tcagtttttc
     1681 cagcatcgcc tggctttgca gggtttccgg aaggtccatc gagatatttt gctggcttaa
     1741 ctggtccacc tcgtcgagga acgctttggt ggtggcatca tcagtatcgt agtaagcacg
     1801 tacccaggtg gagacgttct ccagcgcctg gcgataagtc tcttcctgat gacgcggtac
     1861 agcttgtgct gcgaccagca ggcgagagcg aatattttcg cgcagataga tatcctgatt
     1921 tggcgctaac agcggtacgg cggtgtcatc acgacggcga atcgtaatga agttgtccat
     1981 aaagttctgc cagctttttt gcagattgat acgccattcg ctgatggaac tggaaagctc
     2041 ttcaccgtct gaatccatcg gcgaaccatc gctgtcatta tcggccagac gcaggttatc
     2101 tacctgattt gaaagctgat taagcttaag gatgatgccg tcataatcca cctgcgatac
     2161 tgcagaaagg ctggcgatat catcggtaat tgcccgacga acggtaatca gactcgggtc
     2221 attcatatcc gccaggctgg cgtctgcact tttcagcaac gctgcagagg tcgtgacgtc
     2281 ctgatcgctc cacagcttcc gtccggcgag tttcaccaga aaatcggcct gagccagcag
     2341 ccaggtttta gcatcgctgc cggaaatggt ggcgatcttt tgttggactt catccaactg
     2401 ttttgccagc gtttcttgct gacgattcgc ctgcttaagc tgcgcagctt gttgcttaat
     2461 aatgccttcc agctcggctt tttggctctc ctgggctttt tgcaatgccg tcagttggtt
     2521 agccagggca tcgctggtgg cggtctgatt gacggcctgt tgtttacccc agccatacaa
     2581 accgacaccc gccgccagag caatagcgat agccaccgcg ctgagaatca atgcagtatt
     2641 gttcttactc tttttttctg ttgcgacagg ttgtgacgtg gtgtccacgg cctccctagt
     2701 ctcttcaacc acggcggagg ttttttcttg ttccgtcatt atggcttcct gttatgagag
     2761 ttattgtaat gcccgtaaaa gcgcatcgtt gtcagcgtta tcggcgacct taatgtcttg
     2821 ccagcccagt tcccgggcga gtttcgccaa acgctcactg acgaccaata gtcgacagtg
     2881 tagtaaccag tgctcacgat accattgtgg gatcagcgac cagagttgct gcaacatttc
     2941 accgctggta acaacgacca tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc
     3001 accatcgtaa tggattgcgc atcgttgata acattcacaa aaagtgacct cagcaccgcg
     3061 caccgtcagg gtatccccaa ttagctcacg accgccattg ccacgtaata tcagcgcacg
     3121 tttgcccgca atattttgta attcaggtaa ttgtagcaag acttcgctga tttcccgatc
     3181 ctgtgggtag agaatcttct gtccgcttac ggtatgtagc gccagtgcgg tggtgcgtcc
     3241 aatggcgaaa taagtaggta gttggggcca tttacgatct tgctgatgca gctgtgattg
     3301 ggcaaaagca accgcgtgtt gcgagagggc aaacaacaga tcgctctccc ccagcgctgc
     3361 cagttgatca gcaagttgcg gtaattgtcg acccggagaa aactcaatca gcggaaaatg
     3421 ccaggccacc tgccccagtg tgcgcagacg gctcactaac tcttctccag cgggagacgg
     3481 gcgggtgacc aggatactca tgccggggcc tctccgttat agacttcagc gaggatctcg
     3541 cgcgcgccgt tattcagtag ctcttctgcc agcgaaatcc ccatttgttc ggcatcttgc
     3601 ggcgcaccgc ggcgttcacc gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca
     3661 cgtagccaga tttcgccatc aataagctcg gcgtagctac caattggcac ctgacatccg
     3721 ccttcgagac gggtattcat ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga
     3781 ttcagcgcgg caagcagctc gcgagtgcgt gtatcatcaa ggcggcattc aataccgact
     3841 gcaccttgtc ctaccgccgg aagagaaatc tcgggtggta acgcggcgcg aatgcgggac
     3901 tccagaccta aacgttttag tccggctacg gcaagaatga tggcatcgta ttcgccgtta
     3961 tctagtttgc tcaggcgagt gccgacgttg ccgcgcaggg agcggataat cagatccggg
     4021 cggcgttcag ccagttggca ctggcgacgt aaactggacg tcccgacgat actgcctgct
     4081 ggtaacgcat ccagactgtc atagttattg gacacaaagg catcgcgagg atcttcacgc
     4141 tcgcaaatag tgaccagccc cagaccttgc gggaattcaa ccggcacatc tttcattgaa
     4201 tgtacggcga tatcagcgcg attctccagg agcacgactt ccagctcttt tacaaataag
     4261 ccttttccgc ctactttcgc cagcggcgta tcaagaatca catcgccgcg cgtcaccatc
     4321 ggtaccagtt caacgaccag gcccggatgg ctcgccatca acttgtcttt gacatagtgt
     4381 gcctgccaga gtgcaagtgg gctttggcgt gtggcaattc ttaaaacatt gtctaacatg
     4441 cttgttaccg tcattatcat ccgtggtcca tcctaacatc cttgccagag tgatgtcagt
     4501 gttgtggtga aacgtagacg cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg
     4561 acaggcgttt cacgccgtcg taataaggaa tttacagaga ataaacggtg ctacacttgt
     4621 atgtagcgca tctttcttta cggtcaatca gcaaggtgtt aaattgatca cgttttagac
     4681 cattttttcg tcgtgaaact aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga
     4741 catacgaaat atcccgaatg ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga
     4801 cgggtagcaa atcaggcgat acgtcttgta cctctatatt gagactctga aacagagact
     4861 ggatgccata aatcaattgc gtgtggatcg cgcgcttgct gctatggggc ctgcattcca
     4921 acaggtctac agtctactgc cgacattgtt gcactatcac catccgctaa tgccgggtta
     4981 ccttgatggt aacgttccca aaggcatttg cctttacacg cctgatgaaa ctcaacgcca
     5041 ctacctgaac gagcttgaac tgtatcgtgg aatgtcagta caggatccac cgaaaggtga
     5101 gcttccaatt actggtgtat acaccatggg cagcacctcg tccgtagggc aaagttgttc
     5161 ctctgacctg gatatctggg tctgtcatca atcctggctc gatagcgaag agcgccagtt
     5221 actacaacgt aaatgcagcc tgctggaaag ctgggccgcc tcgctgggtg tggaagtcag
     5281 cttcttcctg attgatgaaa accgcttccg tcataatgaa agcggcagcc tggggggcga
     5341 agattgtggc tccacccagc atatactgct gcttgacgaa ttttatcgta ctgccgtgcg
     5401 tctcgccggt aagcgtattc tgtggaatat ggtgccgtgc gacgaagaag agcattacga
     5461 cgactatgtg atgacgcttt acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct
     5521 cggtggctta agctcgcttt ctgctgaaga gtactttggt gccagccttt ggcagctcta
     5581 caagagtatc gattccccat acaaagcggt gctgaaaaca ctgctgctgg aagcctattc
     5641 ctgggaatac ccgaacccac gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg
     5701 cgagattgta tcgtttggcc tcgatccata ctgcatgatg ctggagcgtg ttactgaata
     5761 cctgacggcg attgaagatt tcacccgtct ggatttagta cgtcgctgct tctatttaaa
     5821 agtgtgcgaa aaactcagcc gtgaacgcgc ctgtgtaggc tggcgtcgcg cagtgttgag
     5881 ccagttagtg agcgagtggg gttgggacga agctcgtctg gcaatgctcg ataaccgtgc
     5941 taactggaag attgatcagg tgcgtgaggc gcacaacgag ctgctcgacg cgatgatgca
     6001 gagctaccgt aatctgatcc gctttgcgcg tcgcaataac cttagcgtct ccgccagtcc
     6061 gcaggatatc ggcgtactga cgcgtaaact gtatgccgcg tttgaagcat tgccaggtaa
     6121 agtgacgctg gtaaacccgc agatttcacc cgatctctcg gaaccgaatc tgacctttat
     6181 ttatgtgccg ccgggccggg ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa
     6241 tattgagtcg atcatcagcc atcagccgct ggaatataac cgttacctga ataaactggt
     6301 ggcgtgggca tggtttaacg gcctgctgac ctcgcgcacc cgtttgtata ttaaaggtaa
     6361 cggcattgtc gatttgccta agttgcagga gatggtcgcc gacgtgtcgc accatttccc
     6421 gctgcgctta cctgcaccga caccgaaggc gctctacagc ccgtgtgaga ttcgccatct
     6481 ggcgattatc gttaacctgg aatatgaccc gacagctgcg ttccgcaatc aggtggtgca
     6541 ttttgatttc cgtaagctgg atgtcttcag ctttggcgag aatcaaaatt gcctggtagg
     6601 tagcgttgac ctgctgtacc gcaactcgtg gaacgaagtg cgtacgctgc acttcaacgg
     6661 cgagcaatcg atgatcgaag ccctgaaaac tattctcggc aaaatgcatc aggacgccgc
     6721 accgccagat agcgtggaag tcttctgtta tagccagcat ctgcgcggct taatccgtac
     6781 tcgcgtgcag caactggttt ctgagtgtat tgaactgcgc ctttccagca cccgccagga
     6841 aaccgggcgt ttcaaggcgc tgcgcgtttc tggtcaaacc tggggcttgt tcttcgaacg
     6901 cctgaatgta tcggtacaga agctggagaa cgccatcgag ttttatggcg ctatttcgca
     6961 taacaaactg cacggcttgt cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt
     7021 ggtggacggc tttgccagcg aagggatcat ccagttcttt ttcgaagaaa cgcaagacga
     7081 gaatggcttt aatatctaca ttctcgacga aagcaaccgg gttgaggtgt atcaccactg
     7141 cgaaggcagc aaagaggagc tggtacgtga cgtcagtcgc ttctactcgt catcgcacga
     7201 ccgcttcacc tacggctcaa gcttcatcaa cttcaacctg ccgcagttct atcagattgt
     7261 gaaggttgat ggtcgcgaac aggtgattcc gttccgaaca aaatcgattg gcaacctgcc
     7321 gcccgccaat caggatcacg atacgccgct attacagcag tatttttcgt gatgagcgtg
     7381 ccggaaagcg aggcttatcc ggcatgcaat cttagcggaa actgactgtt tcacccgcct
     7441 gttgcgtcgc cgcctgttcc agcaaatccc agaaggtttc gccgctgcga tcgcaaatcc
     7501 attcatcgcc tttcaggtca aaatggtagc cgccctgttt ggttgccagc cacacctggt
     7561 gcagcggctc ctggcggtta ataatgattt tgctgccgtt ttcaaaggta atggtcagta
     7621 cgccgccgtt gatttcgcag tcgatatcgc tgtcgccatc cctgtcgtcc agacgctctt
     7681 caatggtcag ccacagttga tcagccaggc gatgaaattc actgtcgttc attgttgtat
     7741 cctgttttta agtgatggcg gcagtatagc ggcatggggt cagggcttca aagtttgcac
     7801 ctctgctgct gcgttccggc atgattcatc cgtcaccgga ataatgatgt ctctgttttg
     7861 aacttcacgt acgttctcct ctctgacacg agttttcgcc gggcaggggg agggcgtaat
     7921 ccttacctct gtatcggtcc atgtataaac attgcgatcg tcataagcga agcgatcgtt
     7981 tttagttagc ggaccgttaa ctggaataat ttgcaagctc tttgaccgta attggcggat
     8041 aaagtaagca tgctgtttgc cgctattaaa tgagcgatca atgaccaggt ctggcgtatc
     8101 cagcgggatt atctcaagct ggtcttcacc aaatgtcgat aattttgtta catacagccg
     8161 atctttatat tgcgcaatat tgccagtaat cagatggaat tgttcaggtt caatacctgg
     8221 taggtctatc cacttgctcc actgacggtc gggcgtgagc tgtttacgcc agcgaacgcg
     8281 actttcccct acttcaaaag agacgtcatc atctacgggt atcgctttgc ctgataattg
     8341 accaaaggga taacgatgca gacccttatt gtcgcgaaaa accagcagtg aatgaggtat
     8401 ccagcgaatt atctcaaatg agtctgcatt cgccgtaagg tgaacgccat ttaaataaat
     8461 attctgccca ctgcggataa gcagatcatt gggatatgaa tttctgttta cactcataag
     8521 acgtggatta ataccccacc atcttttttc taatacagta acatcactgc tgctcccctg
     8581 acgtcgaccg gacaagtaga gatttttact atccaccagc gtggtagagt taccttcgat
     8641 agcttttagt gtcgccagat caacacgtga cgcgccgcta ttactttcgg tgcgctgtcc
     8701 gtcaaaatag aggctatatt tatcgaccgc gaagcggccc atagcctgaa agctggcaat
     8761 atcgacagtg ggtgttcccg gtggattgct aacgattttg ccacgccaga gaataacgcg
     8821 gccgtctgtc acccagtggt attcctcatt ttctatgctt atttcatcgg aaggtatata
     8881 acccgttttc ccagagatat ttttcacaag gcgcaaatcg gccctgctca tctccggaaa
     8941 ctgggcaatg attttcttat ccccccaata acctttaccg taagacataa caccgtgatc
     9001 attgtaatag tcgaacccta tggcctgtac agcaggcgtc agaaatgtgt gtcccaacag
     9061 gatgagtgca actgtgatta tttgaccctt cattttgtcg cctcattgcg cggggcagga
     9121 ataaattcgc cgtcatcagt aatttttccg tccagatatc ccgttttgta atttacgcca
     9181 tagccatatc ccgaatgttg ttccttccag attaagcgaa cttgtttcgg atcagcggtg
     9241 cggtatcgcc agagctgcgc gccatcaaag ctgtaaatgt agcggttatc gcgggcatac
     9301 catctgcgca aaaagggatg gtcattaaga tgggcttcgc gttcatccgg agaacggtta
     9361 tccagtaaga tcagcggtcc gtcagattca aacacgtgta aaccggaatg gtatggccac
     9421 tcttcggcac gcgtaagtaa atagccgtgg cgtttcccgg cgttgaaacg tttgttgata
     9481 ataagattgg gatcatcaag tttcacgatc tccagcttac ggtcaccaga ctctgtttcg
     9541 atgatggtat aaagcgagtc ctgatactgg gcgacagcat cactgatggg ttgaaactgc
     9601 tccggatcga gtccgggtaa ttcttcctgt ttacaagcag gtcctttacg ccagaacact
     9661 ttcttttcca gcagagaaaa atccagacag tgctttgtta aatcttcatc ccacgccaga
     9721 ttttccttgt cgaggacttt acgctgcagc ccgtttttat cacgccaggt caagaatgag
     9781 ccgggcatcc agcgcacgac ggaaaaggtg tccgggtcgg catcaagctg ttcgccgttg
     9841 agcaggattt tggtccgtgt acgagccagg gtatcccagg ggccaaatgg tacggcaatg
     9901 caggggttaa acgtggtgga aaactttcct cgctgatcct aggacttctg cgccagcaca
     9961 cggaaactct cggggtcagc gaggcgatgc ccattgatat acaagaaatt agcgtcacgt
    10021 aatatcagcc ccagtaagtc aggatcccag ggaggccgga attccacctg atgcagcgtt
    10081 ttgatatcca agctatttcc accaccattg tcatcggtgc gcttgccttc aaaatagaga
    10141 ctgtgtttat ctgcggcaaa atcaccccat gccttgaacg aggcgacatc gacgggaggt
    10201 gtgccgggag ggttttgcac aacttcaccc gcgtagagga tattgcgacc atcgctatgc
    10261 catgaataat gaggatacca ggcctgtgcg ccctcataga ggtagtcggt tgtcaaatca
    10321 gtcagaggcg gttctttatc ccattcgtct agtgttgtcg ggtctattaa taaattgggt
    10381 aaccgacgca aactcttgta attcacgtca ggtagagaaa gatttgtttc tttataaggg
    10441 cgcaggtaca ccacgccttt tccctgtttc tggtaaatat tatgagccga catccccatg
    10501 ccgacgataa aaacaatgaa caagccaaca aacaaaccac attgcgatag tgcataaagc
    10561 catcctggcg cgaggtgttg atcacgaaac tgccagcaga acataaatcc tcacgagtaa
    10621 gcgttatact cgcagcattt cctcactttt cagacttcat aaagagtcgc taaacgcttg
    10681 cttttacgcc ttctcctgcg atgatagaaa gcagaaagcg atgaacttta caggcaatcc
    10741 ataatgaaaa acgtgtttaa ggcactcact gtattactta ctctcttcag cctgacgggc
    10801 tgcggtctga aaggtccgct ctatttcccg cctgcagata aaaacgcacc gccgccgacc
    10861 aaaccggtag agacgcaaac gcaatccacg gtgccggata aaaacgatcg cgccactggc
    10921 gatggtccat cccaggtgaa ttactaaaag tcagtttctg tacccgcgtg attggagtaa
    10981 atgatgcagt tctcgaaaat gcatggcctt ggcaacgatt ttatggtcgt cgacgcggta
    11041 acgcagaatg tctttttttc accggagctg attcgtcgcc tggcggatcg gcacctgggg
    11101 gtagggtttg accagctgct ggtggttgag ccgccgtatg atcctgaact ggattttcac
    11161 tatcgcattt tcaatgctga tggcagtgaa gtggcgcagt gcggtaacgg tgcgcgctgc
    11221 tttgcccgtt ttgtgcgtct gaaaggactg accaataagc gtgatatccg cgtcagcacc
    11281 gccaacgggc ggatggttct gaccgtcacc gatgatgatc tggtccgcgt aaatatgggc
    11341 gaacccaact tcgaaccttc cgccgtgccg tttcgcgcta acaaagcgga aaagacctat
    11401 attatgcgcg ccgccgagca gacaatctta tgcggcgtgg tgtcgatggg aaatccgcat
    11461 tgcgtgattc aggtcgatga tgtcgatacc gcggcggtag aaacgcttgg tcctgttctg
    11521 gaaagtcacg agcgttttcc ggagcgcgcc aatatcggtt ttatgcaagt ggttaagcgc
    11581 gagcatattc gtttacgcgt ttatgagcgt ggggcaggag aaacccaggc ctgcggcagc
    11641 ggcgcgtgtg cggcggtggc ggtagggatt cagcaaggtt tgctggacga agaagtacgc
    11701 gtggaactcc ccggcggtcg tcttgatatc gcctggaaag gtccgggtca cccgttatat
    11761 atgactggcc cggcggtaca tgtctacgac ggatttattc atctatgaag caaccagggg
    11821 aagaactgca ggaaacactc acggagcttg atgaccgggc ggttgtcgat tatctgatta
    11881 aaaatcctga gttttttatc cgcaatgcgc gcgcagtgga agcgatacgt gtgccgcatc
    11941 cggtacgcgg caccgtttcg ttggtcgagt ggcacatggc ccgcgcacgt aatcacattc
    12001 atgttctgga agagaacatg gcgttgttga tggagcaggc tatcgccaac gaagggctgt
    12061 tttatcgcct gctctacctg cagcgcagtc tcaccgccgc cagcagtctc gacgatatgc
    12121 tggtgcgctt tcaccgctgg gcgcgcgatc tcggcctggc tggtgcgagt ctgcgcctgt
    12181 ttccagatcg ctggcgctta ggtgcgccgt cgaaccacac tcatctggca ttaagccgtc
    12241 agtctttcga accgctgcgt attcagcgtt tggggcagga acagcactat cttgggccgc
    12301 ttaacggacc ggagctgctg gtggtgctac cggaagcgaa agcggtggga tcggtggcga
    12361 tgtcgatgct gggaagcgat gctgatttgg gtgttgtgct gtttactagt cgcgatgcca
    12421 gtcactatca acaagggcag ggaacgcagt tacttcatga aattgcgctg atgttgccgg
    12481 agcttctgga gcgttggatt gaacgcgtat gaccgattta cacaccgatg tagaacgcta
    12541 cctacgttat ctgagcgtgg agcgtcagct tagcccgata accctactta actaccagcg
    12601 tcagcttgag gcgatcatca attttgccag cgaaaacggc ctgcaaagct ggcaacaatg
    12661 cgatgcagcg atggtacgca attttgctgt acgcagtcgc cgtaaagggc tgggagcagc
    12721 aagtctggcg ttacggcttt ctgcgctacg tagctttttt gactggctgg tcagccagaa
    12781 cgaactcaaa gctaacccgg cgaaaggagt ttcggcaccg aaagcgccgc gtcatctgcc
    12841 gaaaaatatc gacgtcgacg atatgaatcg gctgctggat attgatatca acgatcccct
    12901 cgctgtacgc gaccgtgcaa tgctggaagt gatgtacggc gcgggtctgc gtctttccga
    12961 gctggtgggg ctggatatca aacacctcga cctggagtcc ggcgaagtgt gggtgatggg
    13021 gaaaggcagc aaagagcgcc gcctgccgat tggtcgcaac gctgtggcgt ggattgagca
    13081 ctggcttgat ttgcgcgacc tgtttggtag cgaagacgac gcgctttttc tgtcgaaact
    13141 gggcaagcgt atctccgcgc gtaatgtgca gaaacgcttt gccgaatggg gcataaaaca
    13201 agggctgaat aatcacgttc acccacataa attacgtcac tcgttcgcta cgcatatgct
    13261 ggagtcgagc ggcgatcttc gtggtgtgca ggagctactg ggtcatgcca acctctccac
    13321 cacgcaaatc tatactcatc ttgattttca acaccttgcc tcggtgtacg atgcggcgca
    13381 tccacgcgcc aaacggggga aataatgcgt ttttaccggc ctttggggcg catctcggcg
    13441 ctcacctttg acctggatga taccctttac gataaccgtc cggtgatttt gcgcaccgag
    13501 cgagaggcgc ttacctttgt gcaaaattat catccggcgc tgcgcagctt ccagaatgaa
    13561 gatctgcaac gcctgcgcca ggcggtacgg gaagcggaac ccgagattta tcacgacgtg
    13621 acgcgttggc gttttcgttc gattgaacaa gcgatgctcg acgccgggct gagtgccgaa
    13681 gaagccagtg caggcgcaca cgcagcaatg attaactttg ccaaatggcg cagccgaatc
    13741 gacgtcccgc agcaaactca cgacacctta aaacagctgg cgaagaaatg gccgctggtg
    13801 gcgatcacca acggtaacgc ccagccggaa ctgtttggtt tgggggatta ttttgagttt
    13861 gtgctgcgcg ctggcccgca cgggcgctca aaaccgttca gcgatatgta ctttttggct
    13921 gcggaaaaac tcaacgtgcc gatcggcgag atcttacatg ttggggacga tctcaccact
    13981 gacgtgggtg gggcaattcg cagcggaatg caggcttgtt ggatcagacc ggaaaatggc
    14041 gatctgatgc aaacctggga cagccgttta ctgccacatc tggaaatttc ccggttggca
    14101 tctctgacct cgctgatata atcagcaaat ctgtatatat acccagcttt ttggcggagg
    14161 gcgttgcgct tctccgccca acctattttt acgcggcggt gccaatggac gtttcttacc
    14221 tgctcgacag ccttaatgac aaacagcgcg aagcggtggc cgcgccacgc agcaaccttc
    14281 tggtgctggc gggcgcgggc agtggtaaga cgcgcgtact ggtgcatcgt atcgcctggc
    14341 tgatgagcgt ggaaaactgc tcgccatact cgattatggc tgtgacgttt accaacaaag
    14401 cggcggcgga gatgcgtcat cgtatcggac aactgatggg caccagccag ggcggcatgt
    14461 gggtaggcac cttccacggg ctggcgcacc gcctgctgcg tgcgcaccat atggacgcca
    14521 atctgccgca ggatttccag atcctcgata gcgaagacca gctacgcctg cttaagcgtc
    14581 tgatcaaagc catgaacctc gacgagaagc agtggccgcc gcgccaggca atgtggtaca
    14641 tcaacagcca gaaagatgaa ggcctgcgtc cgcatcatat tcaaagctac ggtaatccgg
    14701 tggagcagac ctggcagaag gtgtatcagg cgtatcagga agcgtgcgat cgcgcgggcc
    14761 tggtggactt cgccgagctg ctgctgcgcg ctcacgaact gtggcttaac aagccgcata
    14821 tcctacaaca ctaccgcgaa cgttttacca atatcctggt ggacgaattc caggatacca
    14881 acaacattca gtacgcgtgg atccgcctgc tggcgggcga caccggcaaa gtgatgatcg
    14941 tcggtgatga cgaccagtca atctacggct ggcgcggggc gcaggtggag aatattcagc
    15001 gtttccttaa tgatttcccc ggtgccgaaa ctattcgtct ggagcaaaac taccgctcta
    15061 ccagcaatat tctgagcgcc gctaacgccc tgattgaaaa caataacggg cgtctgggta
    15121 aaaaactgtg gaccgatggc gcggacggtg agcctatttc cctctattgc gcttttaacg
    15181 aactcgatga agcgcgtttt gtggttaacc gcatcaaaac ctggcaggac aacggcgggg
    15241 cgcttgccga gtgcgccatt ctctaccgca gtaacgccca gtcgcgtgtg ctggaagaag
    15301 cactgttgca ggcgagtatg ccgtaccgta tttacggcgg gatgcgcttc ttcgaacgcc
    15361 aggaaatcaa agatgcgctc tcgtatctgc gcctgattgc caaccgtaac gacgacgcgg
    15421 cctttgagcg tgtggtgaat acgccaacgc ggggtattgg tgaccggacg ctggacgtgg
    15481 tacgtcagac atcgcgcgat cgccagttaa cactctggca ggcatgtcgt gaactgttgc
    15541 aggaaaaagc cctcgccgga cgtgctgcca gcgccttaca gcggtttatg gaactgatcg
    15601 acgccttagc gcaggaaact gccgatatgc cgctgcatgt acagactgac cgggtaatta
    15661 aagactccgg cctgcgtacc atgtacgagc aggagaaggg cgaaaaaggt cagacgcgta
    15721 tcgaaaactt agaggaactg gtgacggcaa cgcgccagtt cagctacaac gaagaagacg
    15781 aagatttaat gccgctgcag gcgttcctat cccatgcggc gctggaagca ggcgaagggc
    15841 aggcggatac ctggcaggat gcggtacaac tgatgaccat gcactcggcg aaaggtctgg
    15901 agttcccgca ggtgtttatc gtcggtatgg aagagggcat gttcccaagc cagatgtcgc
    15961 tggatgaagg cgggcgtctg gaagaagaac gccgtctggc ctacgttggc gtaacccgcg
    16021 cgatgcagaa actgacgctg acctacgcgg aaactcgccg tctgtatggc aaagaggttt
    16081 accatcgccc gtcgcgcttt attggcgagt tgccggaaga gtgtgtggaa gaggtgcgcc
    16141 tgcgcgccac ggtaagccgc ccggtcagcc atcagcggat gggtacgccg atggtcgaga
    16201 acgacagcgg ctacaagctc ggccagcgtg tacgccacgc taagtttggt gaaggcacca
    16261 tcgtcaatat ggaaggcagc ggtgaacata gccggttgca ggtggcattc cagggccagg
    16321 gaatcaaatg gctggtggca gcttacgccc ggctggagtc ggtgtaacgt tgccggatgc
    16381 ggtgctgcgc accttatttg gcctaaaaaa tcattcagat tcaataaatt gcaacgtcat
    16441 gtaggccgga taaggcgttt acgccgcatc cggcatcagc agcatattta gtatctgaca
    16501 caaagctatc attctaacct ttccgctcga cggaaatcat ggtgacaaaa ggatagcgtt
    16561 gccacgggat tgccccgcct ttcatataca tatgtgaaat cgtgccatca agataaagca
    16621 gctgctcaac gttcagtttc gctttggcat aacaggcaaa atcataaaaa tttgtcgcct
    16681 gctggctcaa caaaaacacg gcattcccat gtttattaat cccaacaccg ttacgaattt
    16741 tacgtgaggc gacgttggga tgaatacgcg gattaattac accgttttcc atcaacattg
    16801 gtcctgactg caccgcaaac tgaatatctt tactggcttt aaaagcatcc agacgaacga
    16861 tgccgacttt atctcccgcg acataaaaca cgccgccagg acggataaag aaattccctt
    16921 cacctgaagc gagatttaac gccaccttc
//
LOCUS       DADLSE010000084        16935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLSE010000084 DADLSE010000000
VERSION     DADLSE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.084"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            190..1323
                     /gene="fepE"
                     /locus_tag="JN692_002674"
     CDS             190..1323
                     /gene="fepE"
                     /locus_tag="JN692_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7264954.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN692_002675"
     CDS             complement(1320..2135)
                     /gene="fepC"
                     /locus_tag="JN692_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7264955.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN692_002676"
     CDS             complement(2132..3124)
                     /gene="fepG"
                     /locus_tag="JN692_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HAY7264956.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN692_002677"
     CDS             complement(3121..4125)
                     /gene="fepD"
                     /locus_tag="JN692_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HAY7264957.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4236..5486
                     /gene="entS"
                     /locus_tag="JN692_002678"
     CDS             4236..5486
                     /gene="entS"
                     /locus_tag="JN692_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HAY7264958.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN692_002679"
     CDS             complement(5490..6446)
                     /gene="fepB"
                     /locus_tag="JN692_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7264959.1"
                     /translation="MRLAPLHRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGAATPNNRVADDQGFLRQWSKVAKERKLQQLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6737..7912
                     /gene="entC"
                     /locus_tag="JN692_002680"
                     /pseudo
     CDS             6737..7912
                     /gene="entC"
                     /locus_tag="JN692_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7922..9532
                     /gene="entE"
                     /locus_tag="JN692_002681"
     CDS             7922..9532
                     /gene="entE"
                     /locus_tag="JN692_002681"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HAY7264960.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGHLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9546..10403
                     /gene="entB"
                     /locus_tag="JN692_002682"
     CDS             9546..10403
                     /gene="entB"
                     /locus_tag="JN692_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HAY7264961.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10403..11149
                     /gene="dhbA"
                     /locus_tag="JN692_002683"
     CDS             10403..11149
                     /gene="dhbA"
                     /locus_tag="JN692_002683"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HAY7264962.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            11152..11565
                     /gene="entH"
                     /locus_tag="JN692_002684"
     CDS             11152..11565
                     /gene="entH"
                     /locus_tag="JN692_002684"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HAY7264963.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11746..13851
                     /gene="cstA"
                     /locus_tag="JN692_002685"
                     /pseudo
     CDS             11746..13851
                     /gene="cstA"
                     /locus_tag="JN692_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
     gene            14033..14230
                     /locus_tag="JN692_002686"
     CDS             14033..14230
                     /locus_tag="JN692_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7264964.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN692_002687"
     CDS             complement(14240..15328)
                     /gene="hcxA"
                     /locus_tag="JN692_002687"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HAY7264965.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15437..16597
                     /gene="ybdL"
                     /locus_tag="JN692_002688"
     CDS             15437..16597
                     /gene="ybdL"
                     /locus_tag="JN692_002688"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HAY7264966.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            complement(16598..>16935)
                     /locus_tag="JN692_002689"
     CDS             complement(16598..>16935)
                     /locus_tag="JN692_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264967.1"
                     /translation="MEIVDGFHHHEIGKGSSSLKLRLKGYLPVTCLEDTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
BASE COUNT         3741 a         4420 c         4843 g         3931 t
ORIGIN      
        1 tacagaaaat aattatgggt tttttatttg tttgatttat aggtttgatg aatatttctc
       61 ttaaatagag tgaatgttgc attcagtgga aggaatttac aaacttcagc ttcggataat
      121 tctcaccaac tggtaaagtg agcgccgtgg agattttccc ctattggact cattttcagg
      181 ttatacccca tgtcatcatt gaatattaaa cagggaagtg acgctcattt ttctgagtat
      241 cctctggcgt cgcccagtaa taatgaaatt gatttactta atctaatcga agttttatgg
      301 cgggccaaaa aaacggtcat ggcggtcgtt tttgcatttg cctgcgcagg tttactgatc
      361 tctttcatcc tgccgcaaaa atggaccagc tcagccgtta ttactcccgc cgaagccatt
      421 cagtggcagg acctggagaa aacctttacc aaactccgcg ttcttgatct cgatgttaat
      481 atcgaccgtg gcggtgcatt taacctgttt atcaagaagt ttcagtctgt tagcttgctg
      541 gaagagtacc tgcgttcatc accttatgtg atggaccaat taaaagaggc gaaaatcgat
      601 gaactggatt tgcatcgcgc tattgttgca ttgagcgaaa aaatgaaagc ggttgatgac
      661 aatgccagta agaaaaaaga tgaaccgtca ctgtatacct cctggacgct aagttttacc
      721 gcgccaacca gtaaagaggc gcagaccgtt ttgagcgggt atatcgatta tatctctgcg
      781 ttggtggtga aagagtcgat agaaaacgtc cgtaataaac tggagatcaa aacccagttt
      841 gaaaaagaaa aactggctca ggatcgcatt aaaacgaaaa atcaacttga tgcaaacatt
      901 cagcgcctca attattcact cgacattgcc aacgcggcag gaattaaaaa gcccgtatac
      961 agtaatggtc aggccgttaa agatgacccc gatttctcta tttctctcgg cgcagacggt
     1021 attgaacgca aactggaaat tgaaaaagcg gttactgacg ttgcggaact gaacggtgaa
     1081 ttacgtaatc ggcagtatct tgtcgagcaa ttaacaaaag caaatgtcaa cgatgtgaat
     1141 tttacgccgt ttaaatatca gttaagcccg tcattgccag tgaaaaaaga cggtccgggt
     1201 aaggcgatta ttgtgatcct ttccgcgctg atcggcggga tggtggcttg tggtagcgtg
     1261 ttattgcgct atgcgatggc atccagaaaa caggatgcca tgatggcaga ccacttagtt
     1321 taactatttg cggttgaagg tgccgttcgt ccgagcggca ccacaagcgg cgttccggct
     1381 accggatcgt caatgatcat gcatcgcaga ccataaatgc gctcaatcag ttcagcagtg
     1441 acaatctcct tcggcgctcc ctgagcaaca attttccctt cccgcaatgc aatcaaatgg
     1501 ctggcgtaac gacaggcctg attaagatcg tgcagcaccg ccgccagggt atagcctttc
     1561 tcgcggttca gttcgcttaa cagttccagt agatcaatct gatgactgat atccagccag
     1621 gtcgtgggtt cgtcgagcag cataattgcc gtttcctggg ccagcaccat cgcgatccac
     1681 gctcgctggc gttgtccgcc agaaagggta tccacgcttt gatctgccag atgagttatt
     1741 cccgtggcct gcatcgcttt cgttaccgct tcttcatcct ctttgcgcca gcgggtaaac
     1801 agcggttgat gcggataacg tccacgcgcc accagctcct gcacggtgat atcgcccggc
     1861 gtggtagcat tttgcgccaa cagaccaatc cggcgtgcaa cttctttact ggcgtaatgt
     1921 tgaatgtgct cgccatccag ccagacatgc ccgtgagcag gcgtcatcag gcggcttaag
     1981 gtacgcagta acgtggattt accacagcca tttggtccga taattgccgt gaagtgacca
     2041 tcaggaattt ctacagtcag attttccgca acggtatatt tgccatatcc cagggttaac
     2101 tgttcgccgc gcaaacgggc tactgattcg gtcatttttt gcgagactcc tgaattaaca
     2161 agacgataag gtaaataccg ccgaggctga cggtaacgac gccaaccgga agttgatacg
     2221 gcataaacag ttgttgggcg cagagatcgg ccgccagcag taacagcgct ccgcacagcg
     2281 ccgcctgggt cagcccccag cgagcggtgc cgctaatgcg tcgggcaatg tgcggcgcga
     2341 ctaaagcaat aaaggaaatc ggcccggcaa gcgccgttgc cgcagcggta agcaccactg
     2401 caaccagcat cattaacaga cgcgaacgct cgacgctgac gcccagcgcg caggcggtat
     2461 catcgcccat ctccagcaag cgcattcgcc gcaccagcaa ggtggcggca atgagcatca
     2521 atataatgat gggtgcggaa ggcgaggttt ttgcccacgt caggccgttg agcgatccgg
     2581 cattccacaa acctgctgtt agcgccgttt ctaaagacgc tttcagcaac agccaggtat
     2641 taaaggccac cagcatggcg cgaacgccga taccgataat aatcaaccga aaggtgtcga
     2701 taccgttacg ccaggcgagc agccagacca acagcgaagt gataatgccg cccaccattg
     2761 ctgccagcgc gatagccgtc agatcctgac caaacagcac catcgccacc agcacgccgc
     2821 tccacgcccc ggtgttaaag cccattacgt cagggctgcc gagcgggtta cgcatcagcg
     2881 actgaaaaat cgcgccgctg acgcccagcg ccgcgccaat caacagcgcc atcagcacgc
     2941 gtggtaaacg ccattcggtg accaccatcg tcatactgcg cggcgcatcg cccatcaacg
     3001 cggcgaatac ctgcgagatt tccagcgtga cggcaccgct gcgtaatccc cagatacctg
     3061 caaccacaca ggcggaaatc agcagcaaac aggtgatgag taatcggcga gagacgtaaa
     3121 tcatgcacca cctcgcgttt tacgtcgcac gaggaagatc agcaccggtg caccaataaa
     3181 cgcactgacc acagaaacgc gcagttcgcc gggcacaatc acccgcccga tgatatcggc
     3241 aaacagcagc agggcagggg tagcaagtag cgtgacgggc agcgaccagc gatgatcggc
     3301 acctaccagc cagcgcgcca tgtgcggcat catcaggccg ataaaggcaa tcgggccaac
     3361 taccgccgtc gcactaccac aaagcacggt aatcgccagc agaccaatca actgtgtgcg
     3421 cgccacgcga ctgcccagcg ccgtcgcggt gtcactgccg aggctcaaac tgttcagcgc
     3481 gcgactcagt aatagcgcag ttgctccggc gatcagaacc gggatcagca ccacttttaa
     3541 ggtatgtaga ttgcgaatat ccagcgaacc ggcttgccag aaacgcagtt gatcgtagac
     3601 gtcaggatta agcagggcga tgccgctggt cagtccttcc agcaccgccg ccagcgccac
     3661 gcccgccagg gttaaacgca ccggacttaa ctgccctcca ccctgactgc cggtaaaggc
     3721 aacaatcaat gaggccacca gcgccccggc gaaggccatc gccagttgtt cctgtgcgga
     3781 tgagtaacca aacagcgccg cacccagcac aatggcaaag ctggctccgg cgttcacgcc
     3841 aagcaagccg gggtcggcaa gtgggtttcg ggtgagggtt tgcattaacg ccccggcaag
     3901 gccaagcgcg ccgcctgcca gtaaaccggc aagggtacgc ggcagccgcg cgtcgagcac
     3961 gatggtgcag tcggcgctct ggcaggtgcc ggagaaggcc tccagcacta cggaagcggg
     4021 gagtgatttt gccccaatga gcaggcttaa tgccgtcgcg ataatcagta ataacagcaa
     4081 tccgggcacg gcaatggcgc gtgtcacggc aacagaacca gacatacgaa cttccatgat
     4141 aatgaaatta attatcgtta tcgatcttat ttggatatgt tagcatgtgc agcctaagaa
     4201 taggtattta aaatatttga tggcaaggca ttgtaatgaa taaacaatcc tggctgctta
     4261 acctcagcct gttgaaaacg cacccggcgt ttcgcgcagt attcctcgct cgttttatct
     4321 caattgtgtc tctgggtttg ctcggcgtcg cggtgccggt gcagatccag atgatgaccc
     4381 attctacctg gcaggtgggg ctttcggtga cgctgaccgg cggcgcgatg tttgttggcc
     4441 tgatggttgg cggtgtgctg gcggatcgct atgaacgcaa aaaagtgatt ttgctggcgc
     4501 gcggcacctg tggcattggc ttcattggac tgtgccttaa tgcactgctg ccggagccgt
     4561 cattgctggc aatctattta cttggtttat gggatggttt tttcgcatca cttggtgtta
     4621 cggcgctact ggcggcaaca tctgcactgg tagggcgtga aaacttaatg caggccgggg
     4681 cgatcaccat gttgaccgtg cgcctggggt cggtgatttc gcccatgatt ggcggtttat
     4741 tgctggcgac cggcggcgta gcctggaact acgggctggc ggcggcgggc acgtttatta
     4801 ccttgttacc gttattaagc cttccggcgt tgccaccgcc accgcagccg cgtgagcatc
     4861 cgttgaaatc attactggca ggatttcgtt ttctgcttgc cagcccgctg gtgggcggga
     4921 ttgcgctgct gggtggttta ttgacgatgg cgagcgcggt gctggtactg tatccggcgc
     4981 tggctgacaa ctggcagatg tcagcggcac agattggttt tctctacgcg gcgatcccgc
     5041 tcggcgcggc tattggtgcg ttaaccagcg ggaagctggc acatagtgcg cgaccagggt
     5101 tattgatgct gctctccacg ctgggatcgt tcctcgccat tggtctgttt ggcctgatgc
     5161 cgatgtggat tttaggcgtg gtttgtctgg cgctgttcgg ctggctgagc gcggttagtt
     5221 cgttgctgca atatacaatg ctgcaaacgc aaaccccgga agcgatgtta gggcggatta
     5281 acggtttgtg gacggcgcag aacgtgacgg gcgatgccat aggcgcggcg ctgctgggtg
     5341 gtttgggcgc gatgatgaca ccggttgctt ccgcaagcgc gagcggtttt ggtttgttga
     5401 ttatcggcgt gttgttattg ctggtgctgg tggagttgcg acgttttcgc cagacgccgc
     5461 cgcaggtgac agcgtccgac agttaatgct taaaacagcg ccttaagccg ttccagcact
     5521 tgcatggcgc tgtagtaatc caggcggaac gtctcggttc ccagcgcata aacctgcttg
     5581 ttttgtaccg caggcaggtg cgcgagcagt ggattggcat aaatcgcatc ggcatctttc
     5641 tgatcaccag cgaacaggaa tagcgattca ccgtttaacc ccgcagccag attttcccca
     5701 ccaagctgaa tgatgtcatg gcgtttgccc tggctttggc tggcgtttaa ccctgcgggc
     5761 aacttcgcca gcgtaaagcc gagttgttcc agcatctgcc cttgtgctga ttccggcgtc
     5821 cagagattgg cactgtgtgc agcggcggta tagacaatgg cagtgaccgg ctgcggcggt
     5881 aatttgattt gctctttcgc cgctgccagt tgcttatcaa actgtgcaat ccgctctgcc
     5941 gcttgtttct catgcccggt aatttcgcca agctgcgtta acagcgactg ccagcttttg
     6001 tcgtcgtaat tgatgattaa tgtcggggcg atggtggaaa gctgatcata cagcgccagc
     6061 gccgaatccc cgccggttgc gctaattaaa atcagatccg gcatttgcgc ggcaacggct
     6121 tcggcgctcg gttcgccgat atagagctgt tgcagtttgc gttctttcgc caccttgctc
     6181 cactggcgta aaaagccctg gtcatctgcg acgcggttat tcggcgtggc cgcgccgctg
     6241 gcgatcaccg gagcatcaat cgccagcagt gatccggtca gggtgacgct ggtggaaaca
     6301 atacgctgcg gctggctttc cagggtatga gtgccccggc tgtcagtaat ctgacgcggc
     6361 cagtcggcgg cctgaactac ggctattcct gaaagcaaaa gtcctgttaa tagaagggcg
     6421 ttgcggtgga gcggggcgag tctcacaagt cagcttcctg ttattaataa ggttaagggc
     6481 gtaatgacaa attcgacaaa gcgcacaatc cgtccctttg gggagagggg gcaaaacggc
     6541 gcaggacatc acattgcgct tatgcgaatt catcaataat gcttctcatt ttcattgtaa
     6601 ccacaaccag atgcaacccc gagttgcaga ttgcgttacc tcaagagttg acatagtgcg
     6661 cgtttgcttt taggttagcg gccgaaaata taaatgataa tcattattaa agcctttatc
     6721 atttgtgggg gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac
     6781 acttgcgccc aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg
     6841 tttcgcccgt ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca
     6901 aaaactcgcc gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt
     6961 cggggcgatt cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctagca
     7021 gtcgttctcc cgtcaggaaa aacagacctc agcccgctgt tttacccgca gccagtcgct
     7081 gaacgtggtg gaacgccagg caattcctga acaaaccacg tttgaacaga tgggtgcccg
     7141 cgccgccgca cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga
     7201 tatcaccact gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa
     7261 cccggttagt tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag
     7321 cccggaactg ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc
     7381 tgcgcgtcgt cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc
     7441 agaaaaagat cgccatgaac atgaactggt gactcaggcg atgaaagagg tactgcgcga
     7501 acgcagtagt gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg
     7561 gcatctcgca actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc
     7621 ctgtctgctg catccgaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt
     7681 tattgctgaa ctggagccgt tcgaccgcga actgtttggc ggcattgtgg gttggtgtga
     7741 cagcgaaggt aacggcgaat gggtggtgac catccgctgc gcgaagcagc gggaaaatca
     7801 ggtgcgtctg tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg
     7861 cgaaacaggc gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag
     7921 gatgagcatt ccattcaccc gctggccgga agagtttgcc cgtcgctatc gggaaaaagg
     7981 ctactggcag gatttgccac tgaccgacat tctgactcgc cacgctgcga gtgacagcat
     8041 cgcggttatc gacggcgagc ggcagttgag ttatcgggag ctgaatcagg cggcggataa
     8101 cctcgcctgt agtttgcgcc gtcagggcat taaacctggt gaaaccgcgc tggtacaact
     8161 gggtaacgtc gctgaacttt acattacctt tttcgcgctg ctgaaactgg gcgtcgcgcc
     8221 ggtgctggcg ctgttcagcc atcagcgtag tgaactgaac gcctacgcca gccagattga
     8281 accggcgtta ctgattgccg atcgccagca tgcgctattt agcggggatg atttcctcaa
     8341 cacatttgtt gcagagcatt cttccattcg cgtggtgcaa ctgcacaacg acagcggtga
     8401 gcataacttg caggatgcga ttaaccatcc ggcagagggt tttactgcca cgccatcacc
     8461 tgctgatgaa gtggcctatt tccagctttc cggcggcacc accggcacac cgaaactgat
     8521 cccgcgcact cataacgact attactacag cgtgcgtcgt agcgtcgaga tttgtcagtt
     8581 cacacaacag acgcgctacc tgtgcgcgat cccggcggct cataactacg ctatgagttc
     8641 gccaggatcg ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag
     8701 cgccacgctt tgcttcccgt tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc
     8761 gccggcagtc agcctgtggt tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc
     8821 ctcgctgaaa ctgttacagg tcggcggcgc acgtctttct gccacgcttg cagcgcgtat
     8881 tcccgctgag attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa
     8941 ctacacccgt cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg
     9001 tccggacgat gaagtgtggg ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt
     9061 tggacacctg atgacgcgcg ggccgtacac cttccgtggc tattacaaaa gtccgcagca
     9121 caatgccagt gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga
     9181 tccagagggt tacatcaccg tgcaggggcg cgagaaagat cagatcaacc gtggcggcga
     9241 gaagatcgct gccgaagata tcgaaaacct gctgctgcgc catccggcag tgatctatgc
     9301 cgcactggtg agcatggaag atgagctgat gggcgaaaaa agctgtgctt atctggtggt
     9361 aaaagagccg ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga
     9421 atttaaatta ccggatcgcg tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa
     9481 agtcgataaa aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga
     9541 acacgatggc tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatattccgc
     9601 agaataaagt tgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc
     9661 aggactattt tgtcagcttc tggggcgaga actgcccgat gatggagcag gtgatcgcga
     9721 atattgctgc gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc
     9781 agccgaaaga gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc
     9841 tgacccgctc gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca
     9901 cggtgctggt gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca
     9961 aagagagtgg acgtaaccag ctgattatta caggggtata tgcccacatt ggctgtatga
    10021 ccaccgcaac cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg
    10081 ctgatttcag ccgtgacgag catttgatgt cgctgaaata tgtggccgga cgttctggcc
    10141 gggtggtgat gaccgaagaa ttactgccag cacctatccc cgccagcaaa gcggcgctgc
    10201 gtgaggtgat tctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga
    10261 tcgactacgg tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc
    10321 atggtgatat cgactttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc
    10381 tactctcccg cgaggtgaaa taatggattt cagcggtaaa aatgtctggg taaccggcgc
    10441 aggtaaaggt atcggctacg ccacggcgct ggcgtttgtt gaggcgggag cgaaagttac
    10501 aggttttgat caagcgttca ctcaggagca atatcccttt gcgaccgaag taatggatgt
    10561 tgccgacgcc gggcaggtgg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact
    10621 ggacgtgctg atcaatgcgg cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa
    10681 agaggactgg cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgttccagca
    10741 aaccatgaac cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc
    10801 cgcacacacg ccgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag
    10861 cctggcgttg agcgtcgggc tggaactggc gggtagcggc gtgcgctgta atgtggtttc
    10921 gcctggctcc accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga
    10981 acagcgtatt cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc
    11041 ccgtccacaa gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat
    11101 caccctacag gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg
    11161 aaacgccatt taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat
    11221 ctgggaattg tgtatacccg tctgggagat gatgtgctgg aagccgaaat gccggttgat
    11281 acccgtactc atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa
    11341 acgctgggat cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg
    11401 gaacttaacg ccacccatca tcgcccggtg tctgaaggca aggtacgcgg cgtctgccag
    11461 ccgctgcatc ttgggcggca aaatcagagc tgggaaatcg tcgttttcga tgaacagggg
    11521 cggcgttgct gcacttgtcg gctgggtacg gcagttttgg gatgagcccg acagaattaa
    11581 atgagattgc aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata
    11641 ttggtttcaa ctccgattta catggttacc gtgttgttaa atcgcacaaa gatgttatag
    11701 aaacaaaatg taacatctct atggacacgc acacggataa caactatgaa caaatcaggg
    11761 aaatacctcg tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct
    11821 ttaaatcgtg gggaacagat caacgtgctg tggattgtgg tggcgtcggt ctgtatctat
    11881 ctgatcgctt accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg
    11941 acgcgtatga cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag
    12001 aaagtgctgt tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggtgccg
    12061 gtgctggcag cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt
    12121 ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc
    12181 tcgctgggtg agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta
    12241 gcctgcttta tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc
    12301 ctgactcata gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt
    12361 atgggaatct acctgcgcta tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc
    12421 ctggtgttcc tgattttcgc cattatctct ggcggctggg tggcagaaag tccgacctgg
    12481 gcaccgtact ttgactttac cggcgtgcag ctgacctgga tgctggtggg ctacggtttt
    12541 gtggcggcgg tgctgccggt gtggttgctg ctggccccgc gtgactatct ctcgaccttc
    12601 ctgaaaatcg ggactatcgt cggtctggcg gtaggcattt tgattatgcg cccgacgctg
    12661 accatgcctg cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaacctg
    12721 ttcccgttcc tgtttatcac catcgcctgt ggtgcggtgt ctggcttcca tgcgctgatc
    12781 tcttccggca ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac
    12841 ggtgggatgt tgatggaatc cttcgtggcg attatggcgc tggtttctgc ctgtatcatc
    12901 gatccgggcg tgtattttgc catgaacagc ccaatggcgg tgctggctcc ggcagggacg
    12961 gcggatgtag tcgcttctgc cgcgcagatg gtgagtagct agggctttag cattacacca
    13021 gatacgctca accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc
    13081 ggtgcgccga cgttggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg
    13141 atggatgtgg cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg
    13201 gcggtagatg cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg
    13261 tctcctggcc tgaagcggac cgattcactg cctgctaacc tgctggcaac ggcgctgtgc
    13321 gtactggcgt ggggctactt cctccatcag ggcgtggtcg atccgctggg cggtattaac
    13381 actctgtggc cgctgtttgg tattgccaac cagatgctgg cagggatggc gctgatgctc
    13441 tgtgccgtgg tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca
    13501 acggcctggc tgctgatttg taccctgacc gctgactggc agaaagcgtt tagcccggat
    13561 gcgaaagtcg gcttcctggc cattgctaac aagttccagg caatgatcga cagcggcaat
    13621 attccgtcgc agtatactga gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat
    13681 gccgggttaa ccatcttctt tatggtggtc gtggtggttc tggcactgtt ctcgattaag
    13741 acggcacttg cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagccg
    13801 atgccggaaa atgtcgagga gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt
    13861 gaggggggat gttggcgaat gttggcatag tgccgagggt tccccctcac cctaaccctc
    13921 tccccgatgg ggcgagggga ctgaccgagt gcgttgatag catttgtagg ccggataaag
    13981 cgttcacgcc gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga
    14041 ttcactggca aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc
    14101 tgattacgac aactatgtcg aacatatgcg ggttaatcat cccgatcaaa cgccgatgac
    14161 ctacgaggag tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg
    14221 ctgctgctaa atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca
    14281 acgtatctgg tgtcagcgtg actggcaggt aatgaatgga ctccaccggg cgcaaggtgt
    14341 gggcaatcac tttgtcgatc tccgcctgat tattgatatc cacctccagc tccgccagcg
    14401 tagtcggcag atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac
    14461 ccagcaaggc gctttgcacc agaattccgt aagcgacttt ggtgccgtga agaaacttct
    14521 cggtttgcgg caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa
    14581 aacgatcgcc cagaccaccg accatcccac caccagcaat aatagcatcc accacatcgc
    14641 aaaatgattg cgttaactgc tgattttgct gatcggcaag tgcctgttcg ctactgttta
    14701 acaagacgtc gcgaatggcc tgcgcattat tgatccccag ccgcacggtt agcggcaacg
    14761 tttctggttg cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc
    14821 ccgccagcag atattgttgc ggtgcattga ggataatctc cggttccacc agcaccataa
    14881 aattggcgtc gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg
    14941 agagcggtgt ccaggctgcg caggtggcgg cgatcgtcgg agcggcaaca aatggcagac
    15001 cgagacggcg ggcgagggct ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca
    15061 ccacgctgcg atcgtcaccg gactcagccg ccagttgctg tacatcgctt tcgctgcaat
    15121 gaccacgaaa caaaatatgc tttgcccctg gaagtccaaa cgctggcgga agtttggttt
    15181 gcgccgcagc aatggcgcgt ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag
    15241 tgaaaaaatt gtgcaggtga ttgaaacttc ctggatgtga aaagtagtta gccgggccga
    15301 cgaccacgcg gataggattg tgaggcatgt tgtgttgtcc ttattgtttt atttagacat
    15361 ctaaacgtct tgattgccaa atactagcat cgtgttatag tgccttcaac acgcaacttc
    15421 gtcaggtaca ataaaaatga caaataaccc tctgattcca caaagcaaac ttccacaact
    15481 tggcaccact attttcaccc agatgagcgc gctggcgcag caacaccagg cgattaacct
    15541 gtcgcaaggc tttcctgatt ttgatggtcc gcgctattta caggagcggc tggcgtacca
    15601 cgttgcccag ggggcaaacc aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc
    15661 gattgctcag aaaacggaac gtttgtatgg ctatcaacca gatgccgata gcgatatcac
    15721 cgtaacggca ggggcgacgg aagcgttata cgcggcgatt accgcactgg tgcgcaatgg
    15781 cgatgaagtg atttgttttg atcccagcta tgacagttac gcccccgcca tcgcgctttc
    15841 tgggggaata gtgaagcgta tggcactgca accaccgcat tttcgcgttg actggcagga
    15901 atttgccgca ttattaagcg agcgcaccag actggtgatc ctcaacactc cgcataaccc
    15961 cagtgcaact gtctggcagc aggctgattt cgccgctttg tggcaggcga tcgccgggca
    16021 cgagattttt gtcattagcg atgaagtcta cgagcacatc aacttttcac aacagggcca
    16081 tgccagtgtg ctggcgcatc cgcagctgcg tgagcgggca gtggcggttt cttcatttgg
    16141 caagacctat catatgaccg gctggaaagt gggttattgt gttgcgccag cgcccatcag
    16201 cgccgaaatt cgcaaggtac atcagtatct gaccttttcg gtgaataccc cggcacagct
    16261 ggcgcttgct gatatgctac gtgcagaacc tgagcattat cttgcgttac cggactttta
    16321 tcgccagaag cgcgatattc tggtgaatgc tttaaatgaa agccggctgg agattttacc
    16381 gtgtgaaggt acatactttt tgctggtgga ttacagcgcg gtttctaccc tggatgatgt
    16441 tgagttttgc cagtggctga cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt
    16501 ttgcgccgat cccttcccac ataaactgat tcgtctctgt tttgccaaga aggaatcgac
    16561 gttgctggca gcagctgaac gcctgcgcca gctttagcta tttaaccgtc caggcgcggg
    16621 aatattgacg gtcggcaaac aactcttgca ggccgttaat ttgcttcagg cgcagcactt
    16681 cgtcgctgtc cattcccagc tctttgccaa ttttattatc gtcccatccc aactggctaa
    16741 gttcacggac gatttccgac atggcggtga tttgatggcg tccacgagcg cgattatgac
    16801 gaatagtcgc ggcgatgcgt tgattacgcg tatcttccag acaggtcacc ggcaaataac
    16861 cttttaagcg taattttaat gatgatgagc ctttaccaat ttcatggtgg tgaaaaccat
    16921 ccacgatttc catgg
//
LOCUS       DADLSE010000085        16899 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLSE010000085 DADLSE010000000
VERSION     DADLSE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.085"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN692_002690"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN692_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7264968.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN692_002691"
     CDS             2219..2767
                     /locus_tag="JN692_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7264969.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN692_002692"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN692_002692"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7264970.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN692_002693"
     CDS             complement(3664..4854)
                     /locus_tag="JN692_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7264971.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN692_002694"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN692_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7264972.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN692_002695"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN692_002695"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7264973.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN692_002696"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN692_002696"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7264974.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="JN692_002697"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="JN692_002697"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7264975.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN692_002698"
     CDS             complement(12586..13353)
                     /gene="ssuB"
                     /locus_tag="JN692_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7264976.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGPDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN692_002699"
     CDS             complement(13350..14141)
                     /gene="ssuC"
                     /locus_tag="JN692_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HAY7264977.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
     gene            complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN692_002700"
     CDS             complement(14153..15298)
                     /gene="ssuD"
                     /locus_tag="JN692_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7264978.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15295..16254)
                     /locus_tag="JN692_002701"
     CDS             complement(15295..16254)
                     /locus_tag="JN692_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7264979.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRTGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN692_002702"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="JN692_002702"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7264980.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVL
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4219 a         4373 c         4327 g         3980 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgtacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgcgatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cgctcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagccca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgat
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgcaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgcgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atggggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccaacgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cgttttacgc ttaagatcat taatgaaata agcccggcgg cgaatacggc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaatcaaat atcccttcct gctttccaat ggtaaccgcg ttgcgcaagg
    10261 cgaactcgaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gataccttta ccacgcgttc
    10381 tggtcgcgaa gtagcactgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa gaacgctttg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtctg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccaccga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtg acctgccgcg actggttcca gctcagtctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgcgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccaatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctacaccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgtcatg atggtagcgc agcgacctgt gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgttg atctctccca tttccgccgt tggtacagcc agtccggtac
    11101 gccggttgtg accgtcaaag acgactacaa tccggaaact gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatcccg ttgcagaaag gtggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgttg
    11461 ggatgcggcg caaagtttgc tggcaaccta catcaagctg aacgtcgccc gtcatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcgg tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcaatacca
    11881 tgaagcaaac aatatgaccg atgcgctggc agcgctttct gcggcggttg ctgcacagct
    11941 gccttgccgt gacgcgctga tgcaggagta cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgtggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggtgcg tttgcgggca gcaacccggc agcgttccat gccgaagatg gcagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tagcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatcttt acgagaagat
    12361 aactaaagca ctggcttgat acttttgagc caaaatgcct gatgcgacgc ttgacgcgtc
    12421 ttatcaggcc tacaactccg ttgaaattgc tggttttgta ggctggataa ggcgttcacg
    12481 ccgcatcagg cagccgtgcc caactgcctg atgcgatgct tgacgtgtct tatcaggcct
    12541 acaagtccgt tgaaaatgct ggttttgtgc gccacatccg gcatattaac catgacgccg
    12601 aatcagttgt tcagaatgcc cacgccgcat tacccgttgc aatacttccg cttccagctc
    12661 cgctaatcgc accgacccta atcgacgcgg acgggggata tccactgtca aatccggacc
    12721 aatttttccc tcttcaatta acaacacccg gtcagccatt gctaccgctt cgctcacatc
    12781 atgcgtcacc aacagtacgg taaagccgtg ctgctgccaa agtgacacaa tcaaatcctg
    12841 catctcgagt cgcgttaagg cgtccagtgc cccgagcggt tcatcaagca acaataaacc
    12901 cggtcgatga atcaacgctc ttgccagcgc cacacgctgt ttctgcccgc cagaaagtgc
    12961 ggcaggccat tccccggcgc gattctccag tcctaccgca gccaacgccc gacgcgcggc
    13021 atcgcgccac tgacctttaa ggcctaaccc aacgttatca atcaccgatt tccacggcag
    13081 cagacgcgca tcctgaaaca tcattcgcgt atcatcctga atttcagcca gcggtgtggt
    13141 gcccgctaac acatcgcctg cggttggcgt ttccagaccg gccagcaggc gcagcagggt
    13201 acttttacca ccaccgctgc ggcccaccac cgccacaaac tgacctgccg gaatatgtaa
    13261 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13321 tggtgtgccc tggttcagac gagcagtatt catatcgtgg cctccttcaa atgataagcc
    13381 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13441 agcagggcgt aaagaataat agcgactacc accacgtctg tttgcaaaaa ctctcgcgca
    13501 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13561 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcatcgggc
    13621 aggatcacat ggataaacag cggtatgccg gataatccat agctacgtgc catctccacc
    13681 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg gaacagcgta
    13741 ccgagcgcca ccagaaagat tttcgcggat tcatcaatgc caaaccacaa aatcaccagc
    13801 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagcctt
    13861 tctccccagc gcgacagtcc gctaatcagt cccagaatca ggcccagcga tccgccaatt
    13921 gaaaagccaa tcagcgcccg ccaggaactg atcgccagat gctgccacag ttcgccgctg
    13981 gcggagagcg tccagaacgc cgtcaccact ccttccggtg aaggcaaaat acgcgtcgac
    14041 agccagccaa ccgaggaggc cagttgccac accgccacga tgcccaccgg taaaaaccag
    14101 ggggcaacgc gcaataacca cttcttcact ggcgttgcca tcatcgtgtt ccttagcttt
    14161 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14221 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccaaccc
    14281 gatacgcttc ttccagatgc gggtagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14341 cgtattcgtt gattcgcgcg gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14401 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14461 tgccgttatg tagcgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14521 cttgtgcttt ggcgatagtc tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14581 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14641 gcccatacgc ggcagctttc gcccgcactt gttcgatttt ctctttaaca agttccggcg
    14701 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14761 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14821 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14881 gccagacttg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gggcggcaac agtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gctgcaacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttcccagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15421 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgt tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 atagggacga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcggca ggcgtcaggt aagtgggctg
    15781 gatatcggtg aatttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatac
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca gcaccatacc
    16141 aatactgcct ttctgatagc ctgtacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatgggcat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agatgggttt gcagatttgg cgtgaactgg ggtctgtgat
    16381 ggtaatcaat taattgtgag tcatcggcaa acacgccgtg caggatctcc tgagctttca
    16441 gtgcgcttaa aactggttta agggcataat cgaccgccag cagatgggcc acggtaccgc
    16501 ccgtcgccag cggtagcaac actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg cgtggcgaca atcagcccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcataaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tctaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaagaa
//
LOCUS       DADLSE010000086        16807 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLSE010000086 DADLSE010000000
VERSION     DADLSE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.086"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..597
                     /locus_tag="JN692_002703"
     CDS             <1..597
                     /locus_tag="JN692_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7264981.1"
                     /translation="GAKYLEITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAG
                     VIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMT
                     LTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPV
                     RCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(633..1589)
                     /locus_tag="JN692_002704"
     CDS             complement(633..1589)
                     /locus_tag="JN692_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7264982.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNHVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN692_002705"
     CDS             complement(1589..2830)
                     /gene="clsB"
                     /locus_tag="JN692_002705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7264983.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPDYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2827..3588)
                     /locus_tag="JN692_002706"
     CDS             complement(2827..3588)
                     /locus_tag="JN692_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7264984.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3721..4131
                     /locus_tag="JN692_002707"
     CDS             3721..4131
                     /locus_tag="JN692_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7264985.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4093..5199)
                     /locus_tag="JN692_002708"
     CDS             complement(4093..5199)
                     /locus_tag="JN692_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7264986.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5210..6343)
                     /locus_tag="JN692_002709"
     CDS             complement(5210..6343)
                     /locus_tag="JN692_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7264987.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGMPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6336..8072)
                     /locus_tag="JN692_002710"
     CDS             complement(6336..8072)
                     /locus_tag="JN692_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7264988.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN692_002711"
     CDS             complement(8065..9063)
                     /gene="hlyD"
                     /locus_tag="JN692_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7264989.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPMTVQFGDEAGHE"
     gene            complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN692_002712"
     CDS             complement(9063..9734)
                     /gene="cecR"
                     /locus_tag="JN692_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7264990.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9963..11327
                     /gene="rhlE"
                     /locus_tag="JN692_002713"
     CDS             9963..11327
                     /gene="rhlE"
                     /locus_tag="JN692_002713"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HAY7264991.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIVARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN692_002714"
                     /pseudo
     CDS             complement(11559..12041)
                     /gene="ybiA"
                     /locus_tag="JN692_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            12161..14311
                     /gene="dinG"
                     /locus_tag="JN692_002715"
     CDS             12161..14311
                     /gene="dinG"
                     /locus_tag="JN692_002715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7264992.1"
                     /translation="MALTVALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIAKKKEKTKSPRRRRR"
     gene            14339..15301
                     /gene="ybiB"
                     /locus_tag="JN692_002716"
     CDS             14339..15301
                     /gene="ybiB"
                     /locus_tag="JN692_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7264993.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15442..16527
                     /locus_tag="JN692_002717"
     CDS             15442..16527
                     /locus_tag="JN692_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7264994.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         3926 a         4450 c         4551 g         3880 t
ORIGIN      
        1 ggagctaagt atctcgaaat tacggcaacc aaatatgatt caccaccaat gtatgtcgcc
       61 atagggttat tattactttg tatgtatggc ttaagtaagg atatcaacaa gataaatgcc
      121 gccatcgcgg gcgtaatata tctgttttta ctctctttgg tggcgattgt cgttgcaagt
      181 ttagttcctg tatatgccat tatcatcgtg ttcagcactg cgggcgcgat gtttttaatc
      241 agtatgctgg ccggtttatt atttaatgtt gatcctggtt ctcaccgttt tatcattatg
      301 atgacgttga cagggttggc cctggtaatc atcgtgaatg cggcattaat gagtgaacgg
      361 cccatttggg taataagttg cttaatgatt gtgttatggc caggcattat ctcgcatgga
      421 cgaaataagc tccttgaact ggcaggaaaa tgccatagtg aagagttgtg gagtccggtt
      481 cgttgcgctt ttacaggtgc attaacactc tattactatt ttatcggctt ctttgggata
      541 cttgccgcga tagctataac gcttgtctgg caaaggcata cgcgtttttt tcattagtcg
      601 taaatgcaga tactaaagaa cttaactaaa cttcacatcg ccgcttcatt tttcgcccgt
      661 agcttcttcg cctggctttc cagtagcagg taacagatca gcgccagcag cagcgggata
      721 aagtaataca ggacgcgata agcgagcagg gcggcgataa tgctcccttt cgaggtatgc
      781 tccccagcca gtagtgcgat aaacaccgct tccagcacac cgatccccgc cggaatatgc
      841 acgatgacgc cagcaatact actaaccagt aacacgccca gtacaaagaa atagttcacg
      901 ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac gctggaaatc
      961 agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc tttgatggtc
     1021 atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat catcagtaag
     1081 ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg caactccacc
     1141 acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt ggtggtaata
     1201 ctgagcgagg aaatccgcgt aatagtgctg cccggtaacc ctagtcggga gtacaaacga
     1261 tagcgcatac caatgccgcc aacccaggta ctgagcgtca ggttgaaggc gtagcagata
     1321 aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa gcgggcgagc
     1381 aggtcatagc agccgtaaat cagatagctg acgaccacca gcccgaccgc actaagcagc
     1441 gcaacgtgat tgtagtcgcg gatgaccttc cagacctctt cccagtccac ttttttggcg
     1501 tagaccacca gcaacacaat caccgcgata aaaaacagcc aggtgaggat cttctttgct
     1561 aagcgccagc gcgggtgtga tttactcatc agggttttac ccccgtgttt tcagtttcta
     1621 cccgatcctg cgtttccatt gtcggttgtg cggggggatc aacctgcgtc agacgtggtg
     1681 tgtgtgccgg aagccagcca accagcgccg ggaagtggcg taaaaagtgg aacgccagca
     1741 cgcttttggt caggttccac caggtgcgtt tgggcagcat ggtttcatcc acctgctgac
     1801 aatctgcggc aataatgccg ttcagattat cgcgcagcgt ctggttaaaa tgacgatcgt
     1861 ggatgatgac atttgcttcg agattcagtg acaaactgag cggatcgaga ttactggacc
     1921 ctactgtcgc ccagtgatcg tccatcaatg ccactttgcc gtggagcggg cggcggcggt
     1981 actcaaaaac ctgaacgccg cctttaacca gatagttata cagcaagcgc gcaccgactc
     2041 tgacaatcgg catatccggt tcgccctgaa tgatcagttt gatccgcacc ccgcgccgtg
     2101 ccgctttacg caaggcgtgt aaaaatcgat agtcggggaa gaagtaggcg ttggcgataa
     2161 tcacttcccg ccgcgcctga gtgagcattt tcagataatg gcgttcaata tcatcgcgat
     2221 gttcttcgtt atcgcgccag accagcaata cctgcgcttc tcccggctgg cggttctctt
     2281 ccgctttgtg atgacgtcgc caccagcgtc gtgccgcgct ctgtccaggc aggttttcca
     2341 gctcaaactg gagaatatct tcgacaatcg gcccttcaag gcgtaccgcg taatcctgtt
     2401 tagcctctgg accgtagctg gacatatgct cggcggagta attcagcccg ccaataaagg
     2461 cgatacgcgc gtcgatcacc acaattttgc gatgcatccg gcgaaacaca ttggtgcgca
     2521 taccaaaaag gcgagggcgg ggatcgtagt agcggaacac tacgccagct gccgtcagtt
     2581 cattgacaaa ctcatcgctg agatccggcg aaccgtagcc atccagcaag acttccgctt
     2641 taaccccgcg ttgcgctgct gccagtagtg ccgcatgcag ctgtttgccg acgttatcct
     2701 caaaccagat aaacgtttca agaatgatgc gttcttgtgc ctcgccaatc gccttaaaca
     2761 ccgcgggata atattgctcg ccgttttcca gcaactggat cttattgcct tcgcgccagc
     2821 tacatttcat aaatgaatct ccgcacttaa aggggcatga tcagaaaggt gtcgccatgt
     2881 ccgcagcggc aacgcggttg gcgcgctggc gctggcattt ttgacgtaga tcctgtccag
     2941 tcgtagtaga ggaaattgca ccggaaacgt gcgcgccggg cgtccgtggg cgcgggtaaa
     3001 aatctcatcc agtccggcct gcacttttaa cggatgatta gctttttgcc gccagtcatt
     3061 gaaatcaccc gccaccaata ccggttcgcc gtccggtagc tcattcaccc attcggcgag
     3121 catcgcaagc tgcgcctgac ggtgcgcctc acgcaggccc agatgtacgc acatcacatg
     3181 aatcgctttt ccggtcatcg gcggcacaat gcggcagtag agcacgccgc gcttttccgc
     3241 accatcgacc gaaacatcgc gattctcata atgttcaatg ggataacgcg acagtacggc
     3301 gttgccgtga tgcccttccg ggtatacggc attgcgaccg taggcaaaat cgctccacat
     3361 agtgtcggcg agaaactcgt agtgcgaggt atcgggccag ttttccacat gcagcggatg
     3421 aacttcgtgc gcgcccatca cttcctgcag gcaaacaata tcggcgctga cggtacgcac
     3481 ggcatcgcga agttccggca aaatgaagcg tcggttaaac gcggtaaagc ctttgtgaat
     3541 gttgatggtg agcaccttga acgaaaattg ttgtgtttga tcgggcataa ttttcctgtc
     3601 tttgcctctt atctcattga aatagtgtag tcggcgtcac aaaaaggtgc ggtcttacgg
     3661 aattttccgt aaagttcggt actctgagta agtagagata aattcttcag gagagaagcc
     3721 atgaagtggc aacaacgtgt tcgtgtcgca acgggtctaa gttgctggca gattatgttg
     3781 catttactgg tagtggcgct gctggtggtg ggctggatga gtaagactct ggttcacgtc
     3841 ggcgtgggat tatgcgcact gtattgtgtc acggtagtga tgatgctggt gtttcagcgc
     3901 caccccgagc aacgctggcg tgaggtggca gacgtgctgg aagagctgac cacgacctgg
     3961 tattttggcg cagcgctgat tgtgctatgg ctgttgtccc gcgtactgga aaacaacttt
     4021 ttgctggcaa ttgcagggct ggcgatcctt gctggcccgg cggtagtgtc tttgctggcg
     4081 aaagataaga agttacatca ccttacgtct aaacatcgcg tacgccgctg accctgtcgt
     4141 ggccgttatc accagtagcg gccacaaact attccacaca atatccagac tcgcatcctt
     4201 caaataaatc tgcttggtaa tgtccgtaaa gtggcgaata gggttaatcc acgtcagatt
     4261 ttgcagccac actggcatgt tttccaccgg cgaaacgtaa ccggaaagga gaatggcggg
     4321 catcataaaa acaaacacgc cgataaacgc ctgctgttgt gtcgagcaga gtgatgaaat
     4381 caacagaccg aatcccacca gcgataaacc gtaaatcacc atcgtaaagt agaacagcgc
     4441 tagcgatccg gcgaagggga tttgatacgc ccagatacca atcgccagca caatggtggc
     4501 ctggaaggtg gcgacaatta acgccggtac ggctttgccg atgaagatct gccaggtggt
     4561 gagcggcgaa accagtagct gatcgagcgt accttgttca cgttcgcggg cgacggaaag
     4621 tgaagtgacg atcattacgc cgatagtggt gatcatggcg atcagcgacg gcaccacaaa
     4681 ccatttgtag tcgagattcg ggttatacca gttgcgtacc accagctcgc tgttgttggg
     4741 tttcggtttt ccttccagca gctcctgctg ataatttttg acgatctgtt gcaggtagtt
     4801 ggcggcaatt tgcgcactgt tggagttacg cccgtcgagg atcaactgca acggcgcggt
     4861 ctggaaggta tccagtttgc gcgagaagtc agccgggaaa cgcaccagta gtaacgcctt
     4921 ttgtgtgtcg atggtcgggc ggatctcctg tgggcttttc agcagcagca catgtgtaaa
     4981 ggcgctggcg cgggcaaaac gttgagtaag ttccaccgaa tgctcgccgt tatcttcatc
     5041 gtagatggcg atggtggcgt tagtcacttc cagcgtggcg gcgaacggga acaggatcac
     5101 ctgaattagc acgggtaaaa tcagaatcgc gcgggtttgt ggttcgcgca gcaacgactg
     5161 caactcttta cggattaacg tccataagcg atgaaacatg ctcttctccc taatccagcc
     5221 gacgtttggt tttcagccac gtcaggccga taaacatcac cgccgaagcg atcaaaaaca
     5281 gcacgtttac caccagcacc actggaatat tcccggcgag gaacaggctt tgcagggtgc
     5341 tgacgaaata acgagcggga ataatgtacg tcaccgcgcg gatcaccgcg ggcatactgt
     5401 cgatctgaaa aataaagccg gaaagcataa tcgacggcag aaaagcggcg ttcagggcga
     5461 cctgagcggc attgaactgg ttgcgggtaa tcgtggaaat cagcagcccc attcccaggg
     5521 tactgagtaa aaacaggctg gagataaaaa acagaatcag cagcgaaccg cgatacggca
     5581 tgccgagaat aaacactgac accagcatac acagcaacat cgccagcatc ccgagaaagt
     5641 aataagggat cagcttacac agcagcagtt ccgtgcgggt aatctccgta gagagcagag
     5701 cctccatggt gccgcgttcc cattctcgcg ccaccaccag cgaggtgaga atcgcgccga
     5761 tgaccgtcat gataatggtc accgcacccg ggataataaa gtgctggctt atcgccgccg
     5821 ggttaaacca gtagcgggtt tgtacatcaa taagcggttc aaaagtctgc ccgttgtcct
     5881 ccgctcgctg catttgccag atctgccaga tcccttcgac atacccctgc acaaagttag
     5941 cggtattcgg ctcactgccg tcggtgatca cctgaatcgg tgcggtggcg ttggcgcgct
     6001 ccatctgttc ggcgaaatcc acgggaataa ccaccagacc gcgaattttc cccgactgca
     6061 ttttggcgat cagttcctga cggttatcgc tgatggtggc gtcgatgtag ggcgaaccgg
     6121 tcatggtgtg ggtgaaatcc agcgccgctt cgctacgttg ttccagcaaa atcccgaccc
     6181 gcagcttgct ggagtcgagg ttaatgccgt aaccaaaaat aaacagcagt agcagtggga
     6241 tcactaccgc aatcagccag ctactcggat cgcgaacgat ctgccgcgtc tctttgacgc
     6301 acagcgcccg cacgcgacgc caggaaagga tcgggttact cattgctatg ctccttatcc
     6361 cagtcgtgga tcaactgaat aaaggcttgc tccatggtgg gatcgggttg ctcatcgtta
     6421 gctgactgtg ctttcaaatc gtccggcgta ccgctggcga ttaatttccc acggtacacc
     6481 aggccgatgc ggtcgcaata ttccgcttca tccataaagt gggtggtgac catcaccgtg
     6541 acgcctttct ctaccatgct gttgatgtgc agccaaaatt cacggcgggt gagggggtca
     6601 acgccggaag tcggttcgtc gagaaagaga atatccggtt catgcatcag cgaacaggcc
     6661 agcgccagcc gctgtttaaa acctaatggc agttcatcgg tggcgtgtga ggcaatactt
     6721 ttcaggccaa atgcctcgct catacgggag attttttcgt tctgcgcccg accgcgtaag
     6781 ccatacacac cagagaaaaa gcgtaaattc tgttcgaccg tcaggttgcc gtagagcgaa
     6841 aatttttgcg ccatatagcc gagatgctgg cgcgctttac cggaactctc tttcagatcc
     6901 atccccagca ccagcgcctg gccggaagtc ggcaccagca aaccgcacat catcttaaag
     6961 gtggtcgatt tacccgcgcc gtttggcccc agcaaaccaa aaatctcccc acgtttaacg
     7021 gcaaagttga cgtgatcggt ggcggcaaaa tcaccaaatt tcttggtcag ttctttcgct
     7081 tcgatcaccg tctcgccggg tgtgccttct accgtatgca atattgcacc cagcggcgat
     7141 tctgaggttc cggcaccgcc cagcaaatca ataaacgcat cttcaaaacg cggcgtagtt
     7201 tcgttgatgt tgatttccgg catcccgtcg gcatggcgaa tatcgtctgg tgtggcctct
     7261 tttttgagga tcagacgtac cgatttcccc tgaatcatgc cgtcgctgac ctgcggcagt
     7321 ttcaaggcgc gttgcaacag tttgcggttg ccctcgtgtg gactggtcat cagaaagctg
     7381 cgtccggcca tggtttgtgt cagggctttt ggttctccct gatacagcaa ctcgccttcg
     7441 ttcatcagta acacgtcacg gcactgctcg gcttcgtcga gatacgaggt actccagagg
     7501 attaacatcc cttcgcccgc cagctcatgc accatctgcc acagttcgcg ccgtgagata
     7561 gggtcaacgc cgacgccggg ttcatcgagc agcaacactt tcggttcgcc caccagggta
     7621 caggccaggc cgagtttttg tttcatccca ccggagagct tgcccgccag gcgtccggta
     7681 aacggcccaa gagacgtaaa ctccagcagg cgagcaaaag tttgcttacg tgcctcgccg
     7741 gtgacgctgc gcaaatccgc gtacagattg aggttctcca tcaccgtgag atcttcatac
     7801 agaccaaatt tctgcggcat atagccgagc acggcgtgca gcgcgccgtc gtttttgatc
     7861 ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt tcagtaatcc cgccaacatc
     7921 cgcatcagcg tggttttacc tgcaccgtcc ggccccacca accccgtcac ataaccggcg
     7981 tgaatggtac aatcgagcgg cgcgacggcg ggcttgtcca tgcccggaaa gcgtttttcc
     8041 aggccgttca gcgtgataac ggcatcattc atgtcctgcc tcgtcaccga attgtaccgt
     8101 cattggcatt ccctggcgta acgcatcatc ggcgtcggtc accacaatac gcaggcgata
     8161 gacgaggtcg gtacgcagat ccggtgtttc gacggttttc ggggtaaatt cagcagtcgg
     8221 cgaaacgaaa ccaatctgcc cgtgatacgg cttgtccggg cgaccatcgg tataaagcag
     8281 cactttgcgc cccggctggg cctggtcaag attccgttca tcaacataag cgcgcaccca
     8341 caccggacgc gttagtgaaa cggtaaacac cgtgccacct tcattgagaa ccgtgcctgg
     8401 ctctaccgcg cgcgttaaca gcgtgccatc agacggtgct atcaacgttg agtcctgtaa
     8461 attcagctcc gcctgcgcca gttgcgcctg cgcctgttcg aggctggctt tcgcctgagc
     8521 gatgtcctgt tcacggttac cggaacggta ctgacgcaat ttatcctgtg ctgatttcag
     8581 cgttgcctgt gcctggtcgc gcgaggagcg ggcattttcc aggtcatttg ccgaaatggt
     8641 gcggcttttc cacagtcctt gctggcggtt atagaagttc tgcgcatagt cataggcggc
     8701 ttgcgcctgt ttcaccgctg cggcggcctg agcgatttct tcatcgcgat acccggcaag
     8761 catcagatca tactgcgcct gtgccaccga gacgcccgct ttcgcctgca tcagggcaat
     8821 ctcatacggc ttgtgatcca gttcgcccag cacctggccc gctttgatag catcaccttc
     8881 gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa ctaagattca ccgtacgaat
     8941 atccacgttg ccatacagcg tcaggccgtt atcctggcgg ctttgatacc accagtagcc
     9001 tccggcaacc acggcggcaa gtaccactac cgccagtccg atcacgacag gttttttcat
     9061 cactacaaac tcctttgcga taatccttgc aaaatcaggt cgatatgaca ggtcaccgtc
     9121 tggttgatca gttcggtctt ttcttcatcg aacgcggtcc agccggtacg taacagaatt
     9181 gtttctttgc caagacggaa cgccagaatc tcaccaatca acgcatgggt atgaaggatc
     9241 atgcgggtgt cattagcgtc gcagccggtc caggcggcaa tcaggcgtgt caggtggctg
     9301 tgtagcggac taatcacctg ttcgtgcacc aggtggtagg ctgccgtggg agagagctgc
     9361 tcacgggaga taaacttgct gaggttgacg gtgtcatcct gggtgagcag cttaatcatg
     9421 ttcctgcagg cgcgaaggat cagttcacgg atggcagccc gatcaggctg tggttgggcg
     9481 aacaagcgtt cggcttcctc ggcatgcgga cggaactgct cgccaataaa atcggcaatc
     9541 cactgggcgc aggcgaggta caaatcttct ttcgaaccga agtagtaggt gatggcagcg
     9601 atattctgcc cggcctgggc ggctatctcg cgggtggtgg cgttcattcc atattcacca
     9661 aactgcgcca gtgcggcagc aatcagctgt tttttcgcct gttcaccctt gattgtcatg
     9721 gcaggattat tcatcgcaca gtccattctt aatcaaatga ttgattaaga ttatgactcc
     9781 atatgggagt tgtccagtat ggctaagaat tttagctacg ccagtcacag ggataattta
     9841 tgcgctgcgt cacaaaaact gctacactcc gctccctcat gacattgtgg tttttgtcat
     9901 tttccttttc agtatctccc tgaaaactac accggtaacg gtcggggcgg ttcggagtag
     9961 ttatgtcttt cgattctttg ggtttaagcc ctgatatcct gcgcgccgtt gccgagcagg
    10021 gttaccgtga acccacccct attcagcagc aggcgatccc tgcggtgctg gaaggccgcg
    10081 acctgatggc tagcgcccag accggcaccg gcaaaacagc gggctttacg ctgccgctgt
    10141 tgcaacacct gatcactcgc cagccgcacg ccaaagggcg tcgtccggta cgtgcgctca
    10201 ttcttacccc gacccgtgaa ctggcggcac agattggcga aaacgtccgt gattacagca
    10261 aatacctgaa cattcgttcg ctggtggtgt ttggcggtgt cagcattaac ccacaaatga
    10321 tgaagttgcg cggcggcgtt gatgtgctgg tggcaacgcc gggacgtttg ctggacctgg
    10381 aacatcaaaa tgcagtgaag ctggatcagg ttgaaatcct cgtcctcgat gaagctgacc
    10441 gcatgctcga catgggcttt atccacgata tccgtcgcgt gttaacaaaa ctgccagcga
    10501 aacgccagaa cctgctgttc tctgcgacct tctctgacga tattaaagcc ctggcggaaa
    10561 aactgttgca caacccgctg gaaatcgaag tggcacgccg caataccgcg tctgatcagg
    10621 tgacccagca cgttcacttt gtcgataaga aacgcaaacg cgaattgctg tcgcacatga
    10681 ttggtaaagg gaactggcag caggtgctgg tgtttacccg taccaaacac ggcgctaacc
    10741 atctggctga gcagctcaat aaagatggca tccgtagtgc ggcgatccac gggaataaat
    10801 cgcaaggtgc gcgtacccgt gcgctggctg attttaaatc gggtgacatt cgcgtactgg
    10861 tggcgaccga tatcgttgcg cgcggtctgg atatcgaaga gttgccgcac gtcgtcaact
    10921 atgaactgcc aaacgtaccg gaagattatg tccaccgtat cgggcgtacc ggtcgtgcgg
    10981 ctgctaccgg tgaagcgttg tcgctggtgt gtgttgatga acacaaactg ctgcgtgata
    11041 tcgaaaaact gctgaaaaaa gagatcccgc gcattgcgat tccgggctat gagccggacc
    11101 cgtcaatcaa agccgaaccg atccagaacg gtcgccagca acgtggcggc ggcggtcgtg
    11161 ggcaaggtgg tggtcgcggt caacagcaac cacgccgtgg ggaaggtggc gcaaaatctg
    11221 caagcgcgaa acctgcagaa aaaccgtctc gccgcctcgg cgatgccaaa ccggcaggcg
    11281 aacaacaacg tcgccgccgt ccgcgtaaac ctgccgctgc gcagtaatct tttatgccgg
    11341 gctatgcccg gcatcaggct gatgaacaaa cgcaaaactg cctgatgcgc tacgcttatc
    11401 aggcctacgt gaactctgca atatattgaa tttgcatgct tttgtaggcc ggataaggcg
    11461 tttacgccgc atccggcatt tcacaacaag cacttgtcag caatttgaga acacgggaaa
    11521 aaatttatct gttttaccgc ccataagcca ccgagacgtt acttctctat agccaattgt
    11581 tcgcgcaact ccattaaaag gtagcccagt cgattcttgc ccttaccatt accaccgtct
    11641 ccccagtaag catcgttttc cgtatgctca accagttttg cgggcgcggt tgccagcaag
    11701 agcacacgca gttctgcatg ctgttcgaat ttagcgcgaa gtgctttacg catcacctgt
    11761 tctttaaccg actcccagtt tttacgaagt ggcttggaac gattacgccc catgcgagcg
    11821 gcgaccatgg gggaagaaac ccgacggatc tcttcgcggt atttttcatc aaggaatttt
    11881 tgtgcctgaa aatagtgttc ggaggtgggc taggtattcc cgtcaacctt gatgggccag
    11941 gcggcaaagt tagaaaaatc accgtagtca tcgctggtgc tgtagaaatt tatgatggtg
    12001 tcttgcatga cgtgttggat cctttgtgct cgaacgggca ttaaaccgca ttatgttggt
    12061 ggttattgcg agccgctttc cagaaacaga aaaaccatta cccctgaaaa ccgaaaaatg
    12121 ccacaatatt ggctgtttat acagtatttc aggttttctc atggcattaa ccgtcgcgct
    12181 taaagcgcaa attgccgcct ggtataaggc gcttcaggaa cagatccccg actttattcc
    12241 ccgtgcgccg cagcggcaga tgattgcgga cgtcgccaaa acgctggccg gagaagaagg
    12301 gcggcatctg gcgattgaag cccccaccgg cgttgggaaa acgctctcct atttgatccc
    12361 cggcatcgcc attgcccgcg aagagcaaaa aacgctggtg gtgagtaccg ccaacgtggc
    12421 gttgcaggat cagatttaca gcaaagattt accgctgctg aaaaagatca ttcccgatct
    12481 taaattcact gccgctttcg ggcgtgggcg ctacgtttgt ccgcgtaatc tgacggcgct
    12541 cgccagcact gaacccacgc aacaggatct gctggcgttt cttgacgacg aactgacgcc
    12601 aaataatcag gaagagcaaa aacgttgtgc gaagctgaag ggcgatctcg acacttataa
    12661 atgggatggt ctgcgcgatc atacggatat cgctatagat gatgatctct ggcgtcgttt
    12721 gagtaccgac aaagccagct gcctcaaccg taactgttac tactatcgtg aatgcccgtt
    12781 ttttgttact cgtcgggaga ttcaggaagc ggaagtggtg gtggcaaacc atgcgctggt
    12841 gatggcggcg atggaaagcg aagccgtact gcctgacccg aaaaatctgc tgctggtgct
    12901 ggacgaaggt catcacctgc cagatgtggc ccgggatgcg ctggagatga gcgctgaaat
    12961 caccgcgccc tggtatcgac tacagctgga cttgttcacg aaactggtcg ctacctgcat
    13021 ggagcagttt cgcccgaaga ccatcccacc gctggcgatc cctgaacgtt tgaatgcgca
    13081 ttgtgaagag ttgtatgagc ttatcgcctc gttaaacaac attctcaatc tctacatgcc
    13141 tgccgggcag gaggcagagc accgttttgc gatgggcgaa ctgcctgatg aattgctgga
    13201 gatctgccag cggctggcaa aactcaccga gatgctgcgt ggtctggcgg agttatttct
    13261 taacgattta agtgagaaaa ccggcagcca cgatattgtg cgtctgcatc ggttgatttt
    13321 acagatgaac cgcgcgctgg ggatgtttga ggtgcaaagc aaactctggc ggctggcgtc
    13381 tctggcgcaa tcttccggtg cgccggtgac caaatgggcg acgcgggaag agcgcgaagg
    13441 gcagctacac ctctggtttc actgcgtggg aatacgtgtc agcgatcagc tggaaaggct
    13501 gctgtggcgc agtattccgc acattattgt cacctccgca accttgcgtt cgctgaatag
    13561 tttttcgcgt ttgcaggaga tgagtggtct gaaagagaaa gcgggcgacc gttttgtggc
    13621 gctggattcc ccctttaacc actgcgaaca gggcaaaatt gttattcccc ggatgcgctt
    13681 tgagccttcc atcgacaacg aagagcaaca cattgccgaa atggcggcct ttttccgtga
    13741 acaggtggag agcaaaaaac atctcggtat gttggtactg tttgccagcg gacgggcgat
    13801 gcagcgcttt ctcgactatg tgacggattt acgcctgatg ttgctggtgc agggcgatca
    13861 gccgcgttat cgcttagttg aactgcaccg caaacgcgtt gccaacggtg agcgtagcgt
    13921 gctggtgggc ttacagtcat ttgccgaagg gcttgatttg aaaggtgatc tgctcagcca
    13981 ggtgcatatc cacaaaatcg cttttccgcc catcgacagc ccggtggtga tcaccgaagg
    14041 ggaatggctg aaaagcctca accgctatcc gtttgaggtg caaagcctgc cgagcgcctc
    14101 gtttaacctg attcagcagg ttgggcgact gattcgaagc cacggttgct ggggcgaagt
    14161 ggttatctac gacaaacgct tgctgacaaa aaattatggc aagcgactac tggatgcatt
    14221 accggtattt ccgatagagc aaccggaagt ccctgaaggt atagctaaaa agaaagaaaa
    14281 aacgaaatcc ccacgccgtc ggcggcgtta atgatgtgag tcaggtaagg agtcgtaaat
    14341 ggactatcgc aaaatcatta aagagatcgg gcgcgggaaa aaccacgcgc gcgatttaga
    14401 ccgggatact gcgcgcggtc tgtatgctca tatgctcaat ggtgaagtcc ctgaccttga
    14461 gttgggcggc gtattgattg cgctgcgtat caaaggggaa ggggaagcag agatgctcgg
    14521 cttttacgaa gccatgcaaa atcacaccat caagctgacg ccgcctgcag gcaagccgat
    14581 gccgattgtc atccccagtt acaacggcgc gcgtaaacag gctaacctga cgccattatt
    14641 ggcgattctg ctgcataaac tcggttttcc tgtggtggtt cacggagtta gcgaagatcc
    14701 aactcgcgtg ctgacagaaa ccattttcga actgatgggt attacaccaa cgttgcacgg
    14761 cgggcaggcg caggcgaagc tcgacgaaca tcaaccggtg tttatgcctg tcggcgcgtt
    14821 ttgcccgccg ctggaaaaac aactggcgat gcgctggcgg atgggcgtgc gtaacagtgc
    14881 acataccctg gcgaaactgg cgacgccatt tgccgaaggt gaggcactgc gtctttccag
    14941 cgtttcgcat ccggaataca ttggacgcgt cgcgaagttc tttagcgata tcggcgggtg
    15001 ggcgttacta atgcatggta ctgaaggcga agtgtatgct aatccgcagc gctgcccgca
    15061 gatcaatctc attgaccgtg aagggatgcg ggtgctgtat gaaaaacagg accctgctgg
    15121 tagcgagtta ctgccacaag caaaagatcc ggaaaccacg gcgcagtgga ctgagcgttg
    15181 cctggctggc agcgaagcga ttcccgaatc gctgaaaatc cagatggctt gctgcctggt
    15241 ggctacgggt gaagcggcaa ctatcagcga tggcctggcg cgcgttaatc aggcattttg
    15301 atttttatct cagccggatg agccacgtca tccggctttt accccaccgc aatatgaaat
    15361 tcctgcatct ttattgacct tcccgcgccc ggcgtgcagc ataaaaaaac aacaaacaca
    15421 taacataaac aggagttaac catggaaagt ggtcatcgct ttgatgctca gacgctgcac
    15481 agttttattc aggctgtatt tcgtcagatg ggtagcgagg aacaagaagc gaaattagtt
    15541 gccgatcatt taatcgcggc aaacctggca gggcatgatt ctcatggtat tggcatgatc
    15601 ccaagctatg tgcgttcctg gagtcagggg catctgcaaa ttaaccatca tgccaaaatc
    15661 gttaaagagg cgggggcggc agtcacgctc gatggcgatc gcgcattcgg tcaggtcgcg
    15721 gcacacgaag cgatggcgct ggggattgag aaagcgcatc agcacggtat tgctgccgtg
    15781 gcgctacata actcgcatca tatcggccgt atcggttact gggcggagca gtgttcagcg
    15841 gcggggtttg tctctatcca ctttgttagc gtggtcggta ttccaatggt cgcgccgttc
    15901 cacggtcgcg acagccgctt tggcaccaat cccttctgtg tggttttccc tcgtaaagat
    15961 aatttcccgc tgttgcttga ttacgccacc agcgccattg catttggcaa aacccgcgtc
    16021 gcctggcata aaggcgtccc cgtgccgcca ggttgcctga ttgacgttaa cggcgtgccg
    16081 acgaccaatc cggcggtaat gcaggagtcg ccgttggggt cgctgttgac ctttgccgaa
    16141 cataaaggct acgcccttgc agcgatgtgt gaaattcttg gcggggcgct ttctggcggt
    16201 aaaacgacgc atcaggaaac gttgcaaacc agccccgatg ccattcttaa ctgcatgacc
    16261 actatcatca tcaacccgga actgttcggc gcgccggatt gtaacgcgca gaccgaagcc
    16321 tttgccgagt gggtgaaagc ctcgccgcat gatgacgata agccgatttt gctaccgggc
    16381 gagtgggaag tgaacacgcg tcgcgaacgg caggagcagg ggattccatt ggatgcggga
    16441 agctggcagg ccatttgtga tgcggcgcgg cagattggta tgtcggaaga gacgttgcag
    16501 gctttctgtc agcagttagc cagctaaaaa aaagcccgtc cagtggcgga cgggcaaaca
    16561 agggtaacat aggatcaatg agggttagag catatgcgtc tgtcggcaaa cagacaggga
    16621 aatacttgtg ctggacgtag cgtaaacgcc tgatccgtcc aacggttcgg attttgtagg
    16681 cctgataaga cgcggtagcg tcgcatcagg catctgttgc cggatgcggc ataaacgcct
    16741 tatccgtcct acgaattact tataaattac cgcagtaccg cttaatttat tgttgttggt
    16801 ggcggaa
//
LOCUS       DADLSE010000087        16182 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLSE010000087 DADLSE010000000
VERSION     DADLSE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.087"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..138
                     /locus_tag="JN692_002718"
     CDS             <1..138
                     /locus_tag="JN692_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7264995.1"
                     /translation="FLMALPPVYLQDLPLTLRPLLSNGVLVGILLAVLMDNLIPWERI
                     E"
     gene            160..1305
                     /gene="glxK"
                     /locus_tag="JN692_002719"
     CDS             160..1305
                     /gene="glxK"
                     /locus_tag="JN692_002719"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY7264996.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSPLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(1533..2318)
                     /gene="allE"
                     /locus_tag="JN692_002720"
     CDS             complement(1533..2318)
                     /gene="allE"
                     /locus_tag="JN692_002720"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7264997.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKNGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGTSFVDYLVTLHQNCGNQQCFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(2329..3564)
                     /gene="allC"
                     /locus_tag="JN692_002721"
     CDS             complement(2329..3564)
                     /gene="allC"
                     /locus_tag="JN692_002721"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7264998.1"
                     /translation="MITHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FADAMKACEFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQPLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(3586..4635)
                     /gene="allD"
                     /locus_tag="JN692_002722"
     CDS             complement(3586..4635)
                     /gene="allD"
                     /locus_tag="JN692_002722"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HAY7264999.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLCLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKVAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            4952..6619
                     /locus_tag="JN692_002723"
                     /pseudo
     CDS             4952..6619
                     /locus_tag="JN692_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
     gene            6629..7888
                     /locus_tag="JN692_002724"
     CDS             6629..7888
                     /locus_tag="JN692_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HAY7265000.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            7899..8714
                     /locus_tag="JN692_002725"
     CDS             7899..8714
                     /locus_tag="JN692_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HAY7265001.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            8711..9604
                     /locus_tag="JN692_002726"
     CDS             8711..9604
                     /locus_tag="JN692_002726"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7265002.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9741..10808)
                     /gene="purK"
                     /locus_tag="JN692_002727"
     CDS             complement(9741..10808)
                     /gene="purK"
                     /locus_tag="JN692_002727"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7265003.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(10805..11314)
                     /gene="purE"
                     /locus_tag="JN692_002728"
     CDS             complement(10805..11314)
                     /gene="purE"
                     /locus_tag="JN692_002728"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7265004.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(11432..12154)
                     /gene="lpxH"
                     /locus_tag="JN692_002729"
     CDS             complement(11432..12154)
                     /gene="lpxH"
                     /locus_tag="JN692_002729"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7265005.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(12157..12651)
                     /gene="ppiB"
                     /locus_tag="JN692_002730"
     CDS             complement(12157..12651)
                     /gene="ppiB"
                     /locus_tag="JN692_002730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7265006.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            12825..14210
                     /gene="cysS"
                     /locus_tag="JN692_002731"
     CDS             12825..14210
                     /gene="cysS"
                     /locus_tag="JN692_002731"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7265007.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(14246..14767)
                     /locus_tag="JN692_002732"
     CDS             complement(14246..14767)
                     /locus_tag="JN692_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7265008.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(14875..15087)
                     /gene="ybcJ"
                     /locus_tag="JN692_002733"
     CDS             complement(14875..15087)
                     /gene="ybcJ"
                     /locus_tag="JN692_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7265009.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(15089..15955)
                     /gene="folD"
                     /locus_tag="JN692_002734"
     CDS             complement(15089..15955)
                     /gene="folD"
                     /locus_tag="JN692_002734"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7265010.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAARLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         3763 a         4169 c         4492 g         3758 t
ORIGIN      
        1 tttttaatgg cattaccgcc tgtgtatttg caagaccttc cattaacgct tcgtcctctg
       61 ctcagtaacg gcgtactggt cggaatttta ctggctgttc ttatggataa tcttattccg
      121 tgggaacgca tcgaataatt tgttgaaaaa ggattgataa tgaagattgt cattgcgcca
      181 gactctttta aggagagctt aagtgcagaa aaatgttgtc aggcaattaa agccgggttt
      241 tcgcccctct ttcccgatgc gcactatatc tgtttaccga tagcggatgg cggcgaaggg
      301 acggtggatg cgatggtcgc cgcgacgggt ggcaacatcg tgacgcttga agtctgcggg
      361 ccgatgggcg aaaaagtgaa tgctttttat ggccttaccg gagacgggaa aacagcggtg
      421 attgagatgg cggcagcaag tggcctgatg ctggtcgcgc ctgaaaagcg taatccgttg
      481 ctggcctcca gttttggtac gggggagtta attcgccatg cgctggataa cggcattcgc
      541 catattattc tcggcattgg cggcagtgcg acggtcgacg gcggtatggg catggcgcag
      601 gcgcttggtg tgcgtttcct tgatgccgac ggccaggtgc tggcggctaa cggcggcaat
      661 ttagcgcgcg tggcaagcat tgagatggag gaatgcgatc cgcgtctggc gaacagccat
      721 attgaagtgg catgtgacgt tgataacccg ctggtagggg cacgcggcgc ggcggcggta
      781 tttggcccgc aaaaaggggc aacgccggag atggtcgaag agctggaaca ggggctgcaa
      841 aattacgccc gtgttttaca acagcaaact gaaattaatg tctgtcagat ggcgggcggc
      901 ggcgctgcgg gcggtatggg tattgcggcg gcggtatttc tcaatgcgga tattaaaccg
      961 ggcattgaga ttgtgttgaa tgcggtcaat cttgcgcagg cggtgcaggg ggcagcactg
     1021 gtgatcaccg gggaagggcg cattgactcg caaacggcag gcggtaaagc gccgctgggc
     1081 gttgcgtcgg tggcgaagca gtttaatgta ccggtgattg ggattgctgg tgtattgggt
     1141 gatggcgtgg aagtggtgca ccagtacggc attgacgcgg tattcagcat tttgcctcgt
     1201 ctggcacctt tagccgaagt gctcgccagc ggtgaaacca atctcttcaa cagcgcgcga
     1261 aatattgcct gcgccattaa aataggtcag ggaattaaaa actgactctt gcctttaaag
     1321 cggatgcgat ttatttcgta taagagcgca gtactcatgc cggatgcggc atgagtacta
     1381 tatccttcct gaaaatcgcg caaattctat atattgcaga ggtcatgtag gcctgataag
     1441 cgaagcgcat caggcaatgt tacaaaaaaa gccacggtat aaaccgtggc aaaatccaac
     1501 atagctaaaa ataatcaggc gagtggtatg acttaaatct ctacgtcgcg gttgcaatct
     1561 ttcgagtaaa tatagctgaa cgcttcacca cgccctacgc cataaccagc ctgtaaagaa
     1621 taagcgccca taaagatgta atcgcctttt ttcaccggga tccagttatt gtcgaggtta
     1681 taaaccccct gaccggaaag aatataggca ccatgttcct gaacgtgtgt ttcgatataa
     1741 ccgtggctgg cacctggtgc aaaagagagg atatgcatgt tcatatcaaa acctaactct
     1801 ttgggcagaa aatccagcag gatcacatca tccatacctt catagtgaat gcgttccagt
     1861 tcgctggcat tgccagaaac cagccacggt gcatggcctt ctaccggaac atagcggcgc
     1921 ttatataaaa agatttggct gtcttcggcc tgggcgttaa caaacgtcat taaggagcct
     1981 ggtgggcaat aaagatagcc accttcgctt aaggcaaatg ttttgccttc ggctttggca
     2041 gtgatatttc cagagatcac atacaggaac gtttcaatgc cttcgccacc gaagcactgt
     2101 tggttgccac agttttgatg cagtgtgacc agataatcaa caaaagaggt acccagcttt
     2161 ggcgtggaga ggattgtcgc gtcacaattt tcaaagcccg gaataatatt ttttaccaga
     2221 ccatccgggg ttaacagtgc gaaattaccg tttttaacaa tcgcacggtt agccagtaaa
     2281 tcttcgcggt aaccggtgac gttatttaaa tatcccattt atgactcctt atttctgcca
     2341 ggcaagttga taaagcatga gtgccaacgt tttgacccct tcggcaaggt cagtaatatt
     2401 ggtgcgttcc gccgggttat ggctgatccc gttgatgctg gggataaaaa tcatgcaggt
     2461 tggtacgcga ggcgcgaaaa tttgcgcgtc gtgcccggca ccactgtgca tcacccggta
     2521 attcagtttt tctctttcac acaattctgt cagggtggcg accagctcct tattcatcgg
     2581 cacgggttct tcgtccatcc ataaatcgat atcaatacca atgtccattt catcgcaaat
     2641 cgcccgcatg tcgttttcta acggttgggt gaaatcgcgc agcacggcag cgtcggtatg
     2701 acgacaatca atggtgaacg tggttttacc cggcaccaca tttaccgtat tcgggcgcgg
     2761 ctctactttg ccaaaggtca gaaccagcgg atcgcccatc tttttcgctt tttcgatcga
     2821 ctgatggcaa atgcgactga aagcgtaaac cgtatcacga cgataaccca tcggcgtggt
     2881 gcctgcatgg tttgattcgc cgttcagcgt caccgtatag cgacgctgcc cgacaattgc
     2941 attcaccacg ccaattgatt gcccattact ttccagcaca cagccctgtt caatatgcag
     3001 ttcaacaaag gcttttatat cctgacgcgg agttagtggg gcgttcggca gagtaaattc
     3061 gcaagccttc atcgcatcgg caaaactatt tcctttggca tcacagatat tccgcacgtc
     3121 gtcaggattc gccagcccaa agatattttt actgccccag aagacatacg ggaagcggct
     3181 gccttcttct tctgccatcg ccaccacttc gaccgtacgt aacggtgcgc cgtattgcgt
     3241 tttcaaccag tcaattgcca gccacgccgc cagcgcgccg aattgcccgt cgaggttacc
     3301 gccgttaacc acggtatcga tatgcgaacc gctcagaacc acttcctgtg gatattcggt
     3361 gccactcagg cgaccgtata aattccccac ttcatcgaaa cgtgtttcca gcccacttgc
     3421 tgccattctt tttttaaatt gctgctgggt ttccagccat tccggcgaat aaagtaaacg
     3481 ggtcatccca cccgttgggt cagcgccaaa agaggaaagc cagggcagcg tttcttctat
     3541 agcttgacgg gaatgtgtaa tcataagaaa gtcctgtctc aataattatt gcgcaaaggg
     3601 atttttcgtt tcgtatgacg tgttataaag cgcgtcggaa attaaatact ggtaaatatc
     3661 atcaacaatt tcgatgcctt cgacggcgac tttgcgttgt ttaatatcct gatcctgtcc
     3721 gggataataa acctgattaa aaccgggcgc gggggtaatg gcatttaatt cgcgcatggt
     3781 ctggctaaga tgttgacgga ataattcgct ggaggagaaa aagttcggat taataactat
     3841 atgtaattgc cccaaattac gccctgcgtg taaatcgtca tacatcgaac taacctgtcg
     3901 cccgaacggt aagcagagta agacgcctga gaggacgtca atcatcatca tcaggccata
     3961 cccttttggc ccagcggcgg ggagcagagc atgtaccgcg aacggatcgg ttgttggtgc
     4021 accgttttta tcgaccgccc aggtatccgg gatagacata ttacgcgagc gggcgtcgag
     4081 cacttttccc catgcctgta cggtagtcgc catatcaaag gtaaggatct cgtcgccttc
     4141 tcccggtgcg gcaaaggcca gtgggttagt accgtagtaa atttccgcgc cgccaaacgg
     4201 caccaccatt ggatcggact ggcacatcga aatgccaatt aatccggcgc gggctgcctg
     4261 ctgcacaaaa taagagattg cgccgctgtg acccatccgg ctgataccga ccaccgcaac
     4321 gcctttttgc tgggcggttt tgatggcatg ttccataccc attttcgccg cgacctgccc
     4381 ggcggcattg tcagcatgta aaattgccga gcacggcccg gtttcctcaa gacgaaactc
     4441 cggttcgcgg ttggtgccgc cttttgaaat gcgttccgcg tagtattcca cgcgcaccgc
     4501 gccatgagag tggatccctc tggcatcggc gtaaaccaat acttcagcca cggttgcagc
     4561 gtgctcacgt tttaacccag cctggcagag tttattctcg attagctggt ggagtgtttc
     4621 ccgactaatt ttcatctgtc ttccttttta acgacggtgt gaagcatgac tgcaattaac
     4681 atacagggaa aatatctgga ttatgtgatc cagacaggca aaaaaatata gttagaattt
     4741 atttgataat ccgctcgctt ttaacctggt ttttaaaaca accacgctta ttaaaaaata
     4801 acacgtaata gaatggcggt tatttgaatt cttttattaa taatccttgt gtgatattca
     4861 tcaccttatt tacccgttgt catcgatacc gtaatcgcca cattaacact gctcgtgcaa
     4921 ttgccatggg tgcaattttt aaggagttgt tatgatccac gcctttatta aaaaagggtg
     4981 ttttcaggat tcggtcagtt taatgattat ttcacgaaaa ctcagcgaat cagaaaatgt
     5041 tgatgatgtt tccgtaatga tgggtacgcc cgccaataaa gcgttattag ataccacagg
     5101 tttctggcat gacgatttta ataacgccac gccgaacgat atttgcgtgg caattcgtag
     5161 cgaagcggcg gatgcgggga tcgcgcaggc gattatgcag cagcttgaag aggcgctaaa
     5221 acaactggcg cagggttcag gcagcagcca ggcgttgacg caggtgcgtc gctgggacag
     5281 cgcctgtcag aaattacccg atgccagtct ggcgctgatt tcagtggctg gcgagtatgc
     5341 ggcggagctg gcgaaccagg cgctggatcg caacctcaac gtgatgatgt tctccgataa
     5401 cgtcacgctg gaagatgaaa tccaactcaa aacccgcgcg cgggaaaaag gcttgctggt
     5461 gatggggccg gactgcggta catcgatgat tgccgccacg ccgctggctt ttgctaacgt
     5521 gatgccggaa ggcaacattg gcgtcattgg cgcttccggt accgggattc aggagctgtg
     5581 ctcacagatt gcgctggcag gggagggaat tactcacgcg attggccttg gcgggcgcga
     5641 cctcagccgt gaagtgggcg gcatcagtgc gctgacggcg ctggaaatgc tcagtgcaga
     5701 cgagaaaagc gaagtgctgg catttgtttc aaaaccacct gccgaagctg tgcgtctgaa
     5761 aattgttaat gccatgaaag caaccggcaa accgacggtg gcgctgtttt taggttatac
     5821 cccggcggtg gcacgcgacg agaatgtctg gtttgcctcc tcgctggatg aggccgcacg
     5881 cctggcttgc ctgctttcac gcgttacggc gtgacgtaac gcgataacgc ctgccagcag
     5941 cggatttatt tgcggtttgt ataccggcgg tacgctggct gccgaagcgg cgggattact
     6001 tgccggatac cttggcgtgg aagtcgatga tgcccatcat cacggcatga tgctcgacgc
     6061 cgatggtcac cagattattg acctgggcga tgatttctac acagtcgggc gtccccatcc
     6121 gatgatcgac ccaaccttac gcaaccagtt aattgccgat ctcggcgcta aaccacaagt
     6181 gcgcgtattg ctacttgatg tcgtgattgg cttcggtgcg actgccgatc ctgccgcctc
     6241 gctggtaagc gcctggcaaa aagcctgtgc cgcgcgttca gataatcaac cactgtatgc
     6301 cattgccacg gtgacaggca ctgaacgtga cccgcaatgc cgctcgcagc aaatcgccac
     6361 gctggaagat gcggggatcg cggtagtgag ttcgctgccg gaagccacct tgctggcggc
     6421 agcgttaatt tatccgctct cgcctgccgc acagcaacat acaccgtcat tactggaaaa
     6481 cgtcgccgtg attaacatcg gattacgcag ctttgcgctg gagctacaaa gcgccagcaa
     6541 accggttgtg cattaccaat ggtcgccagt cgccggcggc aataaaaaac tggctcgttt
     6601 attagaacgt ttgcaataag gggttcccat gtttacatca gtggcgcaag ccaatgctgc
     6661 ggttatcgaa caaattcgtc gtgctcgtcc acactggctg gatgtgcaac cggctttttc
     6721 acttatcagc gaactaaacg agggcaaaac actgcttcac gccgggccgc caatgcgctg
     6781 gcaggagatg accggaccca tgaaaggggc gtgcgtgggc gcatgtctgt tcgaaggctg
     6841 ggcgaaagat gaagcgcagg cactggcgat gctggagcag ggggaagtga acttcattcc
     6901 ttgtcaccat gtgaatgccg tcgggccaat gggcggcatt acttctgcca gtatgccgat
     6961 gctggtggtc gagaacgtga ccgacggcaa ccgggcgtac tgcaacctca acgaaggtat
     7021 cggcaaagtg atgcgttttg gcgcttacgg cgaagatgtc ctgactcgcc atcgctggat
     7081 gcgcgatgtg ttaatgccag tattaagcgc ggcgctgggg cgcatggagc gcggtatcga
     7141 tctcacggcg atgatggcgc agggcattac gatgggcgat gagttccatc aacgcaatat
     7201 tgcttcctca gcactgttaa tgcgtacgct ggccccacaa attgctcgcc tcgatcatga
     7261 taaacagcac attgcggaag tgatggattt cctcagcgtg accgatcagt tcttcctcaa
     7321 cctcgcgatg gcctactgca aggcggcgat ggatgctggc gcgatgatcc gcgcaggcag
     7381 catcgttacg gctatgaccc gcaacggcaa tatgttcggg attcgggtaa gcgggctggg
     7441 tgaacgctgg tttactgcgc ctgtaaacac tccgcaaggt ctgtttttca ccggcttctc
     7501 gcaggagcag gcgaacccgg atatgggcga tagcgcgatt accgaaacct ttggtatcgg
     7561 aggtgcggca atgatcgcag cgcctggcgt aacgcgcttt gtcggtgcgg gtggcatgga
     7621 agcggccagg gcggtatctg aagagatggc ggaaatttac cttgaacgca atatgcagtt
     7681 gcagatcccc ggctgggatt ttcagggcgc gtgcctgggg ctggatattc gtcgcgtggt
     7741 agaaaccggc attacgccac tcatcaatac cggtatcgcc cataaagagg cggggatcgg
     7801 gcagattggc gcaggcaccg tgcgggcacc gctggcgtgc tttgaacagg cgctggaagc
     7861 actggctgaa agcatgggga ttggttgagg aacgcgcaat gacgatcatc catcctctgc
     7921 ttgccagcag tagcgcaccg aattatcgcc agtcctggcg gttagcggga gtgtggcggc
     7981 gggcgattaa cctgatgacg gaaagcggcg aactgttaac gctgcatcgt cagggtagtg
     8041 gtttcggccc cggaggatgg gtgcttcgcc gcgcgcaatt cgatgcgtta tgcggtggat
     8101 tatgcggcaa tgaacgacca caggttgtgg ctcaagggat tcgcctcggg cgtttcacgg
     8161 ttaaacagcc acagcgttat tgtttgctgc gtattacgcc gccttcgcat cctcaaccgc
     8221 ttgcagctgc atggatgcaa cgcgcggagg aaaccggact tttcgggcca ctggcgatgg
     8281 cggcaagcga tccgctgccc gctgaattac gccagtttcg tcactgtttt caggccgcgc
     8341 tcaatggcgt taagaccgac tggcggcact ggctgggtaa aggccccgga ttaacgccaa
     8401 gtcatgatga cacgttgagc ggaatgctgc tggcggcctg gtattatggc gctttagatg
     8461 cgcgcgccgg tcgcccgttt tttgcctgtt ccgacaatct tcaactcgtt accacagcgg
     8521 tgagcgtcag ttatttacgt tatgccgcgc aaggatattt cgcctcgcca ctcctgcact
     8581 ttgtccatgc tctgagttgc ccgaaacgta ccgctgttgc gattgattcg ctgctggcgc
     8641 tggggcatac gtcaggggca gatacgctgc tggggttctg gcttggccaa caattattac
     8701 aaggaaaacc atgaaaacac tggttgtagc tcttgggggc aacgccttac tccagcgcgg
     8761 tgaggcgctg acggcggaaa atcaatatcg caatatcgcc agtgctgtac ccgcgctggc
     8821 acgcctggcc cgttcttatc ggctggcgat tgttcacggc aacgggccgc aggtggggct
     8881 gctggcgtta cagaatctgg cgtggaaaga ggtagaaccg tatccgctgg atgtgctggt
     8941 ggcggaaagc caggggatga ttggctatat gctggcgcag agcttgagcg cacagccgca
     9001 gatgccgcct gttacgacgg tgctgacgcg cattgaggtt tcgcctgatg atccggcgtt
     9061 tttgcagcca gagaaattta ttggtccggt ttatcagcca gaagaacaag aggcactgga
     9121 agcggcttac ggctggcaga tgaaacgtga tggtaaatat ttgcgccggg tggtggcgtc
     9181 tccgcaaccg cgtaaaattc tcgacagcga agccatcgag ttgttgctca aagaggggca
     9241 tgtggtgatt tgcagtggcg gcggcggtgt gcctgtgacg gaagatgggg cagggagtga
     9301 agcggtgatt gataaagatc tcgccgctgc gttgctcgcc gagcagatta atgccgatgg
     9361 actggtgatc ctcaccgatg ctgatgcggt atatgaaaac tggggaacgc cgcagcaacg
     9421 tgccattcgc catgccacac cggatgagtt agcgccattt gccaaagccg atggttcgat
     9481 ggggccgaag gtaacggcgg tgagtggtta tgtcagaagc cgtagtaaac ccgcgtggat
     9541 tggggcgtta tcgcgaattg aagagacgct ggcgggcgaa gcggggacct gtatttcgct
     9601 gtagtgatga gagcatccgg caatcggtgc accatgcctg atgcgacgct tgacgcgtct
     9661 tatcaggcct acaatgggta ccggagcggt aagccggata aggcgtttac gccgcatccg
     9721 gcaatcaaat gagcacctgc ttaactgaac ttactctgcg cccaaatcac gccgctggca
     9781 tactctggcg gcagcagcgg gattaaggct tccagcgtcg cagtcagacg cgatgtgtcg
     9841 ctgtcggtca aattcagatg ccccacttta cgccccggac ggacttcttt gtcgtaccag
     9901 tgcagatgca ccagcggcaa tttcagccag tcataattca catcgctacc aatcagattg
     9961 atcatcaccg acggattatt cactactggt tgcggtaacg gcagatcggt aatcgcccgc
    10021 agatgcagct caaactggct gatgctggca ccgttttgtg tccagtgacc gctgttatgc
    10081 acacgcggag ccagttcgtt gatcagcaga ccttgcgggg tgacaaaaca ctccatcgcc
    10141 atcacgccca catagcccag ctcctgcata atcgccgaca gcatctcttc ggcttgcgcc
    10201 tgctgctgtg cgttggcctg cggaaaagcg acgctggtgc gcaaaatacc gtcctgatgc
    10261 aggttatgcg tcagcggata aaacacggtg ctgccatcaa agccgcgcgc gccaaccagc
    10321 gatacttcac cagagaagtt aatgccctgc tcgacaatac attcaccgta acactctgcc
    10381 ggtaactgtt cggtttcatt ggcgcgtaaa ctccattgac cgcgaccgtc atagccacca
    10441 gtgcgacgct taacaatcgc cagttcacct aaacgatcaa acaccgcagg ccactcgctg
    10501 cgatcggcaa gtaactgcca cggcgcggtc ggcaggtgga gcttatcgaa aagctgtttc
    10561 tgagtcagac ggtcagcaat aatcgggaac acatcgcggt tcacaaaggc cggatgacgc
    10621 gccagctcgc gggttaatgc ggtttccggc cagcgttcga tctcagcggt aatcacgctt
    10681 tgttgaaaag gcaccgccgc cggttcagcg tccagcccga ccggccagac agcaatgcct
    10741 aacggttcgc ctgcctgacg cagcatacgg cctaactgcc cgttaccgag gacgcaaacc
    10801 tgtttcatgc cgcacctcgc gggtccgggt tttccagcac ttcgtcggtc tgggctttgc
    10861 gccagtcatt cagacgctgg tgtagctctt tatcgtgagt cgcgagaatt tgtgctgcca
    10921 gtaatgccgc gtttgccgcg ccagctttac caatcgccag cgtacccacc ggaatgccgc
    10981 gcggcatttg tacgatggag tagaggctat cgacaccgct cagtgcggcg ctctgtactg
    11041 gcacgcccag caccggcacc agcgttttgg cggcaatcat gcctggcagg tgcgctgcgc
    11101 cgcctgcgcc cgcaataatc acctgataac cgttctcttc ggcgctttcg gcgaagctga
    11161 acagtttatc gggggtgcgg tgagcagaaa ccacttcaac gtggtgcggg acattcagga
    11221 tttcgaagat ttcgacggcg aactgcatgg tagcccagtc gcttttggac cccatcacga
    11281 tggcgacacg cgccggatta ttgcgggaag acatgcgtct taaaactcct gtggtgcaca
    11341 actctcggct ttagagggca cagagaatag cacggaaaga gagcaaggaa aacggttgcg
    11401 tggctgtgaa atcagcaaag ttgtgggttt tttaaaacgg aaaatgaatc agctcaacgt
    11461 catccgccgt gactttcacc atcgaacctt ccgtatgcca ggcacccagt accacgcgaa
    11521 aagcaggttg ctggttggcg ataagttcat gcaccgccgg gcgatgggta tgcccgtgga
    11581 tcagccattg cacctgatgt ttttccatcg cactgaccac agcgttttgg ttaacgtcca
    11641 tgatcgccag cgatttactg ctgttggctt ctttgctgtt cgcgcgcatt cgcgcggcaa
    11701 tgcgtttgcg cacaaacaac ggcagggcga ggaatagcat ttgcagccag ggtttgtgga
    11761 ctttggcgcg aaaagcctga taacccgcgt catcggtgca cagcgtgtcg ccatgcataa
    11821 tcaacacccg gcgaccataa agttcgagca ccttttcttc cggcaataat gtcatgccgc
    11881 tttcacgggc aaagcgtttg ccgagcagaa aatcacggtt gccatgaatg aaataacagg
    11941 gaacgccaga atcggacacc gctttgatcg ctgccgccat ctggcgatgg agtgggttgg
    12001 gatcgtcgtc gccaatccat gcttcaaaca gatcgccaag aatatacagc gcgtcggcct
    12061 tgcgggcttc ccccgctaaa aaacgcagaa aaccggcggt gatcgccggt tcttccacgc
    12121 agagatgaag atctgcaata aagagtgtcg ccacgattac tcgctaacgg tcacgctttc
    12181 aatgataacg tcttcttttg gcacgtcctg gtgcataccg ttacgaccgg ttgcaacacc
    12241 tttgattttg tctaccacgt ccatgccgtc aaccacttca gcaaacacgc agtagcccca
    12301 accttgcagg ctttcgccag agaagttcag gaagtcgtta tcaaccacgt tgatgaagaa
    12361 ctgtgcagtt gcagagtgcg gagcctgagt acgtgccatt gccagcgtac cacgggtatt
    12421 tttcaggccg ttgttggctt cgtttttgat cggttctttg gtggcttttt gtttcatgcc
    12481 cggttcaaaa ccgccgccct gaatcataaa gccgttgata acacggtgga aaatggtgtt
    12541 gttgtaaaaa ccttcgcggc agtagtccag gaagttttta actgtttcag gtgctttatc
    12601 gtcaaaagtt ttgatgacaa tatcgccgtg attggtgtgg aaagtaacca tttttgcatc
    12661 ctgttccgtt tgattggtgc ttcaacccag ttcgggtcat atatagggtg gtgttatagc
    12721 ataaccgcac gatcggatca tcacgcaatg tatgctgatt cgcgcgggaa atatgggtat
    12781 tatacgcaac tcaattaccc acacatgtct aaacggaatc ttcgatgcta aaaatcttca
    12841 atactctgac acgccaaaaa gaggaattta agcctattca cgccggggaa gtcggcatgt
    12901 acgtgtgtgg aatcaccgtt tacgatctct gtcatatcgg tcacgggcgt acctttgttg
    12961 cttttgacgt ggttgcgcgc tatctgcgtt tcctcggcta taaactgaag tatgtgcgca
    13021 acattaccga tatcgacgac aaaatcatca aacgcgccaa tgaaaatggc gaaagctttg
    13081 tggcgctggt ggatcgcatg atcgccgaaa tgcacaaaga ttttgatgct ttgaacattc
    13141 tgcgcccgga tatggagccg cgcgcgacgc accatatcgc agaaattatt gaactcactg
    13201 aacaactgat cgccaaaggt cacgcttatg tggcggacaa cggcgacgtg atgttcgacg
    13261 tcccgaccga tccaacttat ggcgtgctgt cgcgtcagga tctcgaccag ctgcaggctg
    13321 gcgcgcgcgt tgacgtggtt gatgacaaac gcaacccgat ggacttcgtt ctgtggaaga
    13381 tgtcgaaaga gggcgaaccg agctggccgt ctccgtgggg cgcgggtcgt cctggctggc
    13441 acattgaatg ttcggccatg aactgcaagc agctgggtaa ccactttgat atccacggcg
    13501 gcggttcaga cctgatgttc ccgcaccacg agaacgaaat cgcgcagtcc acctgtgccc
    13561 atgatggtca gtatgtgaac tactggatgc actccggaat ggtgatggtt gaccgcgaga
    13621 agatgtccaa atcgctgggt aacttcttta ccgtgcgcga tgtgctgaaa tactacgacg
    13681 cggaaaccgt gcgttacttc ctgatgtcgg gccactatcg cagccagctg aactacagcg
    13741 aagagaacct gaagcaggcg cgtgcggcgc tggagcgcct ctacactgcg ctgcgcggca
    13801 cagataaaac cgttgcgcct gccggtggcg aagcgtttga agcgcgcttt attgaagcga
    13861 tggacgacga tttcaacacc ccggaagcct attccgtgct gtttgatatg gcgcgtgaag
    13921 tgaaccgtct gaaagcagaa gatatggcag cggcgaatgc aatggcatct cacctgcgta
    13981 aactttccgc tgtattgggc ctgctggagc aagaaccgga agcgttcctg caaagcggcg
    14041 cgcaggcaga cgacagcgaa gtggctgaga ttgaagcgtt aattcaacag cgtctggatg
    14101 cccgtaaagc gaaagactgg gcggcggcag atgcggcacg tgaccgtctt aacgagatgg
    14161 ggatcgtgct ggaagatggc ccgcaaggga ccacctggcg tcgtaagtaa ttgcgcaatt
    14221 gccggatgcg agttttcgca tccggttatc gtctgcgcca ccacaacatt cccatcagta
    14281 gcatccccgg caaccacacc cacatcaatt cggaaataat cacctgatgc ccgtacgctg
    14341 tggtgtaacg agacaatgca aacggcgcga ctttaatcac ctgccaggga gcgaaaaagc
    14401 gttcatctga ccacggccac agccagccaa cgcccttacc gccagtggtt accgaatcca
    14461 gtaagctgtg cgatagcaac gagacggtta aaaacagcca gcagcgaatc agcccagccc
    14521 tgaaccatcg acgtccaatc agcacacata agagcgggac aacaaacgca aacaccagcg
    14581 aatgggtaaa cccgcgatga ccaaaaacat tgccgtaagc aacgccaaat ttaaacgaca
    14641 atacgtcggc atcgggcagc atcgccagga tgattccggc aaataacaga cgcggaggga
    14701 tgactttcga acccaaccct aaaccaatgc aaaggggaac ggcggcgtgc gtaataacgg
    14761 ttggcatgat ggtcgcttcg gcaaaatgtc gatgctatca gcatggatga acggggcgta
    14821 gagggcaaaa gtctgaaaag agaaccggcc tgttgataca ggccgggaaa gggatcaggc
    14881 aacaacctgt acgctgtgtc ctgcaaaact cactgtctga ccggcgacga ttttgcagcg
    14941 tttgcgcgtt tcaaccgcac cgtcgacttt cacctggcct tcggcaatcg cgattttcgc
    15001 ctgcgcgccg ctttcgctcc agccttccag tttcagcaag tcgcacagct caacgtgcgg
    15061 atgtttacct aaagaaaatg tcgccatgtt actcaccctg tggatcatga tattcaacgc
    15121 acgcctgtag cgtgttttca atcagcgtgg caaccgtcat cgggccaacg ccgccgggaa
    15181 caggcgtaat gtatgaggcg cgtttagccg cgtcttcaaa cacgacgtcg cccacaactt
    15241 tgccattttc cagacggttg atgccgacat caatcacaat tgcgccttct ttgatccagt
    15301 caccgggaat aaagcctggc ttgccaacgg caacgatcaa cagatcggca ttttctacgt
    15361 gatgacgcag atttttagtg aagcggtgag tcacggtggt ggtgcaacct gcgagcaaca
    15421 gttccatgct cattgggcga ccgacgatat tcgatgcgcc aatcaccacg gcgttgaggc
    15481 cgaaggtatc aatgttgtaa cgctcaagca gcgtgacgat accgcgcggg gtgcagggac
    15541 gcagacgcgg cgcgcgctgg cacagacgac cgacgttgta aggatggaaa ccgtccacgt
    15601 ctttgtccgg atgaatacgt tccagcactt tgacgttatc aatacccgcc ggtaacggca
    15661 gttgaaccag aatgccatcg atggtgttgt cggcattcag cgcatcgata agctccagca
    15721 gctccgcttc gctggtggtt tccgggaggt cataagagcg ggagacgaac ccgacttctt
    15781 cacaagcctt gcgtttgctt gcgacataaa tttgcgatgc agggttacta cccaccagca
    15841 caacggccag tcctggtgcc cgcagtctgg ctgcaatacg agcctgaact ttttgagcaa
    15901 cttcagagcg cacctgctgc gcaatcgttt taccgtcaat aatctttgct gccatcagag
    15961 agagaattcc atctgttacg taggtcgaag gggatgcgtc tattttgtca gaagcggggc
    16021 gcgctgtcag gtttcgtttc agatttatcg cgtgaagcga cctcttgcga aggttaggcg
    16081 caccgtcgct gaggtcgctg agattgaaag cttcattttt cgtacatgat ggcgttgtaa
    16141 atctggaact gatttatttc cttgtttaag gattaagata at
//
LOCUS       DADLSE010000088        16070 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLSE010000088 DADLSE010000000
VERSION     DADLSE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.088"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1101
                     /locus_tag="JN692_002735"
     CDS             <1..1101
                     /locus_tag="JN692_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7265011.1"
                     /translation="EKVVGLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAI
                     KGVGEGPIEAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRA
                     ALMNSLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGER
                     ETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGN
                     RIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREV
                     MDIDEAREKYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARL
                     RFGATWRVSPSDRLLNDLRGLIGSEQVELEFD"
     gene            1114..2073
                     /gene="accA"
                     /locus_tag="JN692_002736"
     CDS             1114..2073
                     /gene="accA"
                     /locus_tag="JN692_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7265012.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2172..4313
                     /gene="ldcC"
                     /locus_tag="JN692_002737"
     CDS             2172..4313
                     /gene="ldcC"
                     /locus_tag="JN692_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7265013.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4370..4759
                     /locus_tag="JN692_002738"
     CDS             4370..4759
                     /locus_tag="JN692_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7265014.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4824..6122
                     /gene="tilS"
                     /locus_tag="JN692_002739"
     CDS             4824..6122
                     /gene="tilS"
                     /locus_tag="JN692_002739"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7265015.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6171..6431)
                     /gene="rof"
                     /locus_tag="JN692_002740"
     CDS             complement(6171..6431)
                     /gene="rof"
                     /locus_tag="JN692_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7265016.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6418..6618)
                     /locus_tag="JN692_002741"
     CDS             complement(6418..6618)
                     /locus_tag="JN692_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7265017.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6784..7329
                     /locus_tag="JN692_002742"
     CDS             6784..7329
                     /locus_tag="JN692_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7265018.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            7326..7748
                     /gene="arfB"
                     /locus_tag="JN692_002743"
     CDS             7326..7748
                     /gene="arfB"
                     /locus_tag="JN692_002743"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7265019.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7762..8472
                     /gene="nlpE"
                     /locus_tag="JN692_002744"
                     /gene_synonym="cutF"
     CDS             7762..8472
                     /gene="nlpE"
                     /locus_tag="JN692_002744"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7265020.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8627..9451)
                     /locus_tag="JN692_002745"
     CDS             complement(8627..9451)
                     /locus_tag="JN692_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7265021.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9504..11222)
                     /gene="proS"
                     /locus_tag="JN692_002746"
     CDS             complement(9504..11222)
                     /gene="proS"
                     /locus_tag="JN692_002746"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7265022.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYNKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11333..12040)
                     /gene="tsaA"
                     /locus_tag="JN692_002747"
     CDS             complement(11333..12040)
                     /gene="tsaA"
                     /locus_tag="JN692_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7265023.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12037..12441)
                     /gene="rcsF"
                     /locus_tag="JN692_002748"
     CDS             complement(12037..12441)
                     /gene="rcsF"
                     /locus_tag="JN692_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7265024.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12559..13374)
                     /gene="metQ"
                     /locus_tag="JN692_002749"
     CDS             complement(12559..13374)
                     /gene="metQ"
                     /locus_tag="JN692_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7265025.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13414..14067)
                     /gene="metI"
                     /locus_tag="JN692_002750"
     CDS             complement(13414..14067)
                     /gene="metI"
                     /locus_tag="JN692_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7265026.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14060..15091)
                     /gene="metN"
                     /locus_tag="JN692_002751"
     CDS             complement(14060..15091)
                     /gene="metN"
                     /locus_tag="JN692_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7265027.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDDIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15279..15854
                     /gene="gmhB"
                     /locus_tag="JN692_002752"
     CDS             15279..15854
                     /gene="gmhB"
                     /locus_tag="JN692_002752"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7265028.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3905 a         4040 c         4313 g         3812 t
ORIGIN      
        1 gagaaggtgg tgggcctggt ggatgagtgc tggcggatgg ggctgaaaat cctgccacca
       61 gatataaact ccggtcttta ccatttccac gtcaacgacg acggcgaaat cgtgtatggt
      121 attggcgcca tcaaaggggt aggtgaaggt ccgattgagg ccatcatcga agcccgtaat
      181 aaaggcggct acttccgcga actgtttgat ctctgcgccc gtaccgacac caaaaagtta
      241 aaccggcggg tgctggaaaa actgatcatg tccggggcgt ttgaccgtct tgggccacac
      301 cgcgcggcgc tgatgaactc gctgggtgat gcgttaaaag cggcagatca acacgcgaaa
      361 gcggaagcta tcggtcaggc cgatatgttc ggcgtgctgg ccgaagagcc ggaacaaatt
      421 gaacaatcct acgccagctg ccaaccgtgg ccggagcagg tggtattaga tggggaacgt
      481 gaaacgttag gcctgtacct gaccggacac cctatcaacc agtatttaaa agagattgag
      541 cgttatgtcg gaggcgtaag gctgaaagac atgcacccga cagaacgtgg taaagtcatc
      601 acggctgcgg ggctcgttgt tgccgcgcgg gttatggtca ccaagcgcgg caatcgtatc
      661 ggtatctgca cgctggatga ccgttccggg cggctggaag tgatgttgtt tactgacgcc
      721 ctggataaat accagcaatt gctggaaaaa gaccgcatac ttatcgtcag cggacaggtc
      781 agctttgatg acttcagcgg tgggcttaaa atgaccgctc gcgaagtgat ggatattgac
      841 gaagcccggg aaaaatatgc tcgcgggctt gctatctcgc tgacggacag gcaaattgat
      901 gaccagcttt taaaccgact ccgtcagtct ctggaacccc accgctctgg gacaattcca
      961 gtacatctct actatcagag ggcggatgca cgcgcgcggt tgcgttttgg cgcgacgtgg
     1021 cgtgtctctc cgagcgatcg tttattaaac gatctccgtg gcctcattgg ttcggagcag
     1081 gtggaactgg agtttgacta atacaggaat actatgagtc tgaatttcct tgattttgaa
     1141 cagccgattg cagagctgga agcgaaaatc gattctctga ctgcggttag ccgtcaggat
     1201 gagaaactgg atattaacat cgatgaagaa gtgcatcgtc tgcgtgaaaa aagcgtagaa
     1261 ctgacgcgta aaatcttcgc cgatctcggt gcatggcaga ttgcgcaact ggcacgccat
     1321 ccgcagcgtc cttataccct ggattacgtt cgcctggcat ttgatgaatt tgacgaactg
     1381 gctggcgacc gtgcgtatgc agacgataaa gctatcgtcg gtggtatcgc ccgtctcgat
     1441 ggtcgtccgg tgatgatcat tggtcatcaa aaaggtcgtg aaaccaaaga aaaaattcgc
     1501 cgtaactttg gtatgccagc gccagaaggt taccgcaaag cactgcgtct gatgcaaatg
     1561 gctgaacgct ttaagatgcc tatcatcacc tttatcgaca ccccgggggc ttatcctggc
     1621 gtgggcgcag aagagcgtgg tcagtctgaa gccattgcac gcaacctgcg tgaaatgtct
     1681 cgccttggcg taccggtagt ttgtacggtt atcggtgaag gtggttctgg cggtgcgctg
     1741 gcgattggcg tgggcgataa agtgaatatg ctgcaataca gcacctattc cgttatctcg
     1801 ccggaaggtt gtgcgtccat tctgtggaag agcgccgaca aagcgccgct ggcggctgaa
     1861 gcgatgggta tcatcgctcc gcgtctgaaa gagctgaaac tgatcgactc catcatcccg
     1921 gaaccgctgg gcggcgctca ccgtaacccg gaagcgatgg cggcatcgtt gaaagcgcaa
     1981 ctgctggcgg atctggccga tctcgatgtg ttaagcactg aagatttaaa aaatcgtcgt
     2041 tatcagcgcc tgatgagcta cggttacgcg taattcgcaa aagttctgaa aaagggtcac
     2101 ttcggtggcc cttttttatc gccacggttt gagcaggcta tgattaagga aggattttcc
     2161 aggaggaaca catgaacatc attgccatta tgggaccgca tggcgtcttt tataaagatg
     2221 agcccatcaa agaactggag tcggcgctgg tggcgcaagg ctttcagatt atctggccac
     2281 aaaacagcgt tgatttgctg aaatttatcg aacataaccc tcgaatttgc ggcgtgattt
     2341 ttgactggga tgagtacagt ctcgatttat gcagcgatat caatcaactt aatgaatatc
     2401 tcccgcttta tgccttcatc aacacccact cgacgatgga tgtcagcgtg caggatatgc
     2461 ggatggcgct ctggtttttt gaatatgcgc tggggcaggc ggaagatatc gccattcgta
     2521 tgcgtcagta caccaacgaa tatcttgata acattacgcc gccgttcacg aaagccttgt
     2581 ttacctacgt caaagagcgg aagtacacct tttgtacgcc ggggcatatg ggcggcaccg
     2641 catatcaaaa aagcccggtt ggctgtctgt tttatgattt tttcggcggg aataccctta
     2701 aggctgatgt ctctatttcg gtcaccgagc ttggttcgtt gcttgaccac accgggccac
     2761 acctggaagc ggaagagtac atcgcgcgga cttttggcgc ggaacagagt tatatcgtta
     2821 ccaacggaac atcgacgtcg aacaaaattg tgggtatgta cgccgcgcca tccggcagta
     2881 cgctgttgat cgaccgcaac tgccataaat cactggcgca tctgttgatg atgaacgatg
     2941 tagtgccagt ctggctgaaa ccgacgcgta atgcgttggg gattctgggg gggatcccgc
     3001 gccgtgaatt tactcgcgac agcatcgaag agaaagtcgc tgccactacg caagcacaat
     3061 ggccggttca tgcggtgatc accaactcca cctatgatgg tttgctctac aacaccgact
     3121 ggatcaaaca gacgctggat gtcccgtcga tccacttcga ttccgcctgg gtgccgtata
     3181 ctcattttca tccaatctac cagggtaaaa gtggtatgag cggcgagcgt gttgcgggaa
     3241 aagttttctt cgaaacgcaa tcgacccaca aaatgctggc ggcgttatcg caggcttcac
     3301 tgatccacat taaaggcgag tatgacgaag aggcgtttaa cgaagccttt atgatgcata
     3361 ccaccacctc gcccagttat cccattgttg cttcggttga gacggcggcg gcgatgctgc
     3421 gtggtaatcc gggcaaacgg ctgattaacc gttcggtgga gcgggcgctg cattttcgca
     3481 aagaggtcca gcggctgcgg gaagagtctg acggttggtt tttcgatatc tggcaaccgc
     3541 cgcaggtgga tgaagccgaa tgctggcccg ttgcgcctgg cgaacaatgg cacggcttta
     3601 acgatgcgga tgccgatcat atgtttctcg atccggttaa agtcactatt ttgacaccgg
     3661 ggatggacga acagggcaat atgagtgagg aggggatccc ggcggcgctg gtagcaaaat
     3721 tcctcgacga acgtgggatc gtagtagaga aaaccggccc ttataacctg ctgtttctct
     3781 ttagtattgg catcgataaa accaaagcaa tgggattatt gcgtgggttg acggaattta
     3841 agcgctctta cgatctcaac ctgcggatca aaaatatgct gcccgatctc tatgctgaag
     3901 atcccgattt ctaccgcaat atgcgtattc aggatctggc gcaagggatc cataagctga
     3961 ttcgtaaaca cgatctttcc ggtttgatgt tgcgggcatt cgatactttg ccggaaatga
     4021 tcatgacgcc acatcaggca tggcagagac agattaaagg tgaagtagaa accattgcgc
     4081 tggaacaact ggtcggtaga gtatcggcaa atatgatcct gccttatcca ccgggcgtac
     4141 cgctgttgat gccaggagaa atgctgacca aagagagccg cacggtactc gattttctac
     4201 tgatgctttg ttccgtaggg caacattacc ccggttttga aacggatatt cacggcgcga
     4261 aacaggacga agacggcgtt taccgcgtac gagtcctaaa aatggcggga taacttgcca
     4321 gagcggcttc cgggcgagta acgtgctgtt aacaaataaa ggagacgtta tgctgggttt
     4381 aaaacaggtt caccatattg cgattattgc gacggattat gcggtgagca aagctttcta
     4441 ctgcgatatt cttggcttca cgctgcaaag cgaagtctat cgcgaagcgc gcgactcgtg
     4501 gaaaggggat ttggcgctta atgggcaata tgtgattgag cttttctctt ttccgttccc
     4561 gccggagcgc cccagccgac cggaagcttg cggcctgcgt catctggctt ttagcgttga
     4621 tgacatcgat gcggcagtgg cgcaccttga aagccataac gtgaagtgtg aagccatccg
     4681 tgtcgatcca tacacgcaaa aacgcttcac cttctttaac gatccggacg ggctgccgtt
     4741 ggaactgtat gagcagtaag gcttgtcatc gccgcatttg cccggtaacg tgccgggcat
     4801 tgctactgta aaatcgcacc atcatgacac tcacgctcaa tagacaactt ctcacctcac
     4861 gccagattct ggtggccttt agcggcgggc ttgactccac cgttctgctg catcagttgg
     4921 tgcagtggcg gacggaaaat ccgggcgtca ctctgcgcgc tattcatgtg catcacggtt
     4981 taagtgctaa tgccgatgcc tgggttacgc attgcgaaaa cgtctgccaa cagtggcagg
     5041 tgccgctggt ggtcgaacgc gtacaacttg cgcaagaggg gctggggatt gaggcccagg
     5101 cgcggcaggc gcgttatcag gcatttgccc gtaccctatt gcccggcgaa gtgctggtca
     5161 ccgcgcaaca tctcgacgat caatgtgaaa cctttctgct ggcgctaaaa cgcggtagcg
     5221 gccctgccgg gctttcggct atggcggaag tttcggagtt tgctggaaca cggcttatcc
     5281 gcccgttgct tgcccgcacg cggggggaac tggagcagtg ggcattggcg catggtttac
     5341 gctggattga agacgaaagt aatcaggacg actcatacga tcgtaacttt ctgcgcctgc
     5401 gcgtagtgcc gttattgcag cagcgttggc cgcattttgc ggaagcaacg gcccgcagcg
     5461 ccgcactttg tgctgaacaa gagagtctgt tggatgaact gctggcggat gatttagcac
     5521 actgccaaac gtcgcagggg acgctgcaga ttgcgccaat gctggcgatg agtgatgccc
     5581 gtcgcgcggc gattattcgc cgctggctgg cagggcagaa tgcaccgatg ccttcccgcg
     5641 acgcgctggt gcgcatctgg caggaagtgg cgctggcgcg ggaagatgct tccccctgtt
     5701 tacgtttggg cgcctttgaa atccgccgct atcagtcgca actgtggtgg attaaatccg
     5761 tcaccgggca aagcgaaacc attgtgctgt ggcagacgtg gctacaaccg ctggaattac
     5821 cggcggggct gggaaccgta cagcttactg cgggaggcga tattcgccct ccgcgtgcag
     5881 acgaagcggt cagcgtgcgt ttcaaagcgc caggattgct gcatattgtc gggcgcaacg
     5941 gcggacgtaa gctgaagaaa atctggcaag agttaggcgt gccgccgtgg ctacgtgaca
     6001 ccacgccact gctgttttat ggcgaaacgc tgattgcggc ggcaggggta tttgtgacgc
     6061 aagaaggtgt ggctgaaggt gagaatggcg taagttttgt ctggcagaaa acgcttagtt
     6121 aagtgaaagc cggataagac gcatcaaacg tcgcatccgg cgaaagtcaa tcaggattcg
     6181 cttaccacca ccgtaccgat ttcagggtgg ctaaagctgg taattttatc cagacgcagc
     6241 tcgcgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg ggagactaaa
     6301 tcactggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
     6361 tgctggcagg cgagctcaag attatcgtaa tcatcacaat taattggttg atacgtatca
     6421 ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
     6481 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
     6541 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
     6601 ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt ggatgcaagt
     6661 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
     6721 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
     6781 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
     6841 aaccagtttc tcgatgcctc tctgacgctg gcgcgccacc cttcagaaac ccaggagcgt
     6901 atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca atttacccgt
     6961 ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct gggcattgat
     7021 ttgtggattg agctggggct gccggatgag cgacgaatta agaaagcctg cacccaggcc
     7081 gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg gcagcaaaat
     7141 cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga tgaacaactg
     7201 gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat tcaggatggt
     7261 gttatctggt tatcggatga taagaataat ctggaagtta acttaaccgc ctggcaacaa
     7321 ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt gagatcaccg
     7381 ccattcgtgc gcagggcgct ggcgggcagc atgttaataa gacctcaacg gctattcatc
     7441 tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcgt ctgctcgccg
     7501 ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag gaataccgca
     7561 gtcaggaact gaaccgcgaa gccgctctgg cccggctggt ggcagtgatt aaagatttaa
     7621 caaaagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
     7681 tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
     7741 gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
     7801 agcctcttta ctctgatggg atgtaataat cgggccgaag tcgatacgct ttctccggcg
     7861 caggctgccg aactgaaacc gatgccgcaa agttggcgcg gcgtgctacc gtgtgccgat
     7921 tgcgaaggaa tcgaaatctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
     7981 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
     8041 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
     8101 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat
     8161 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
     8221 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
     8281 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
     8341 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
     8401 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
     8461 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct
     8521 tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata
     8581 aggcgtttac gccgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc
     8641 caacaaagcg acccgccttg atataaatcc caggcttcag gtgcccaaca tactgtaact
     8701 gcgtttctgg cttaaacgca gagacatcac caatacgcat atgcatcaga tcggcagggc
     8761 taatccagcc cgattgcgcc agtgtaagtg gatgcccggc tttggcaaat gcatcagtga
     8821 caaattccga acaaaaccac gactttttgt ctccttcgca cacactgctt aactgcgctt
     8881 tcgccaggcc gctgacgcac tgttggcgaa aatcctcgga gaacggattc agtgagcaca
     8941 tctggcgagt caccataaag ggaataaatt cgacaatgcc gcgatagtta taaccgctat
     9001 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
     9061 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acaatctgga
     9121 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag aaagattgca acgtgactca
     9181 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
     9241 gcaaatcgcc gggtttgaga tcaggtgccg ttatttcttt tattgattgt tcggtgaaag
     9301 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
     9361 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
     9421 gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaagggccct
     9481 gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg
     9541 tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg
     9601 ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca
     9661 gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca
     9721 cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc
     9781 atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat gccgcgttcg
     9841 tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc
     9901 atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag
     9961 tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta
    10021 ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg
    10081 gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat gttagcacca
    10141 gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt
    10201 accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc ttcggtcgcg
    10261 aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga
    10321 tcaccgcgca ccagcagcgc aaccagcggg aagctactgc cttcaaccgc tttaaccagc
    10381 agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt
    10441 ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc
    10501 ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc
    10561 acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata
    10621 gaaccggtgt cggcctgtac ggcgcggaaa tccagcccca tgcggctgaa gattttgttg
    10681 taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag
    10741 taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg
    10801 tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc
    10861 tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc
    10921 tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct
    10981 tgccacaaat cggctggctg aaccaccggc atcgacacct cgatcgcacc ggcgttgttc
    11041 atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag
    11101 gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg
    11161 atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc
    11221 atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa
    11281 agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg
    11341 gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat
    11401 gtagccagac ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgcgggt
    11461 cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca
    11521 aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt
    11581 gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat
    11641 ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt
    11701 tatggcaaac aacctctttc agctctacca gcgacatgcc aattgggtta gggcggaaag
    11761 tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag
    11821 tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga
    11881 atgcttccag gccgcgaacg gcgtcggcct ggttgtagga agcaatgaga tgcagttcgc
    11941 cgttggcgct ttttaccaga cctggctggc gcggaacggc gaatttttcc ttatagggcg
    12001 agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc
    12061 agagccgata catacagcct gacgatagca gcccggcgta ccgctggtga cttcgcagct
    12121 atgcagtaat acagcattgg ctttcatttt agaggcattg atttgcatct gcttacgtgc
    12181 ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc
    12241 accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg
    12301 cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg
    12361 aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa
    12421 acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa
    12481 tcaggagctt gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt
    12541 gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc
    12601 tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc
    12661 tttgttatct tcacgcgtta cgatcaggtt tacgtatggg gagtctttat cttcgacaaa
    12721 gataccgtct ttcgccggag tcaggccaat ctggctggca taggtggtat tgataactgc
    12781 cagagcgatt tgcgcgtcgt ccagagaacg cggcagttgc ggtgcttcca gttcaacaat
    12841 tttcagattt tttgggttct caacaacatc aagaacggtc ggcagcaggc caacgccatc
    12901 tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg
    12961 atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt
    13021 ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg
    13081 atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc
    13141 tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag
    13201 gccatatttg tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac
    13261 aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc
    13321 cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta
    13381 ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat
    13441 gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt
    13501 catcaccgtc gcgttgtagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc
    13561 ggcaccgact gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc
    13621 cgcattcacc agacccggca acgcttccgg taacagcact ttacggacga tctgcattgg
    13681 cgtggcaccc attgcgcggg aagcttcaat taacccggtt gggatctcca gcagagcgtt
    13741 ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa ttgctgcctg
    13801 caaaccaatc gatgtaccga caataacgcg ggtaaacgga atcatccata ccagcaagat
    13861 aatgaacggg atggaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc
    13921 gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat
    13981 cacaaaccca aaaaatccgg atacgaaggt cattgccagc gtttcccata cgccacgaac
    14041 cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct
    14101 gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga
    14161 tgccgaattt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt
    14221 tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg gtaaactcca
    14281 gacgcagcat cggcacgcag tcagtaaatg gctccgcttg cagacgttcc tggtaatctt
    14341 ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat
    14401 gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca
    14461 cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac
    14521 ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca
    14581 gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa
    14641 tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc
    14701 caagaccaac caatgacagc aattccgtca cgcgacgttt gatatcgtct ttcggtgtgt
    14761 tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa
    14821 aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg
    14881 tggtcaattc ctggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta
    14941 cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc
    15001 cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga
    15061 acactttggt gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt
    15121 cgtagcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc
    15181 cagacgtcta aatcaatcag gtttatgcga agaacacttt cttgcaggtc gaaacatgcg
    15241 atactagcgt cccatgcctt attaaggagc tataaaaggt ggcgaagagc gtacccgcaa
    15301 tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca
    15361 actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg
    15421 cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt
    15481 ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg
    15541 gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc caggtctgcg
    15601 attgccgcaa accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata
    15661 tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg gttgcggcga
    15721 acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg
    15781 cggcagattg ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa
    15841 aaccggcgca atgattaaaa gatgaacggt tgaaataaaa atgcattttt ccgcttgtct
    15901 tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca
    15961 caaacagccg gttcggttga agagaaaaaa tcctgaaatt cagggttgac tctgaaagag
    16021 gaaagtgtaa tatacgccac ctcgcgacag tgagctgaga gctgcgtcgc
//
LOCUS       DADLSE010000089        15990 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLSE010000089 DADLSE010000000
VERSION     DADLSE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.089"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..116)
                     /locus_tag="JN692_002753"
     CDS             complement(<1..116)
                     /locus_tag="JN692_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HAY7265029.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNGESALIIKMNDYGD"
     gene            complement(243..974)
                     /gene="rlmB"
                     /locus_tag="JN692_002754"
     CDS             complement(243..974)
                     /gene="rlmB"
                     /locus_tag="JN692_002754"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7265030.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     TGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1154..3595)
                     /gene="rnr"
                     /locus_tag="JN692_002755"
     CDS             complement(1154..3595)
                     /gene="rnr"
                     /locus_tag="JN692_002755"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7265031.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPNQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3634..4059)
                     /gene="nsrR"
                     /locus_tag="JN692_002756"
     CDS             complement(3634..4059)
                     /gene="nsrR"
                     /locus_tag="JN692_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7265032.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4264..5562)
                     /gene="purA"
                     /locus_tag="JN692_002757"
     CDS             complement(4264..5562)
                     /gene="purA"
                     /locus_tag="JN692_002757"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7265033.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5666..5863)
                     /locus_tag="JN692_002758"
     CDS             complement(5666..5863)
                     /locus_tag="JN692_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7265034.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5945..6949)
                     /gene="hflC"
                     /locus_tag="JN692_002759"
     CDS             complement(5945..6949)
                     /gene="hflC"
                     /locus_tag="JN692_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7265035.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWCISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6952..8211)
                     /gene="hflK"
                     /locus_tag="JN692_002760"
     CDS             complement(6952..8211)
                     /gene="hflK"
                     /locus_tag="JN692_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7265036.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8297..9577)
                     /gene="hflX"
                     /locus_tag="JN692_002761"
     CDS             complement(8297..9577)
                     /gene="hflX"
                     /locus_tag="JN692_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7265037.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9653..9961)
                     /gene="hfq"
                     /locus_tag="JN692_002762"
     CDS             complement(9653..9961)
                     /gene="hfq"
                     /locus_tag="JN692_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7265038.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10047..10997)
                     /gene="miaA"
                     /locus_tag="JN692_002763"
     CDS             complement(10047..10997)
                     /gene="miaA"
                     /locus_tag="JN692_002763"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7265039.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10990..12837)
                     /gene="mutL"
                     /locus_tag="JN692_002764"
     CDS             complement(10990..12837)
                     /gene="mutL"
                     /locus_tag="JN692_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7265040.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIAPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSLAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12847..14184)
                     /gene="amiB"
                     /locus_tag="JN692_002765"
     CDS             complement(12847..14184)
                     /gene="amiB"
                     /locus_tag="JN692_002765"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7265041.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRSEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQEQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14203..14664)
                     /gene="tsaE"
                     /locus_tag="JN692_002766"
     CDS             complement(14203..14664)
                     /gene="tsaE"
                     /locus_tag="JN692_002766"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7265042.1"
                     /translation="MMNRVIPLPDEQVTLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14636..>15990)
                     /gene="nnr"
                     /locus_tag="JN692_002767"
     CDS             complement(14636..>15990)
                     /gene="nnr"
                     /locus_tag="JN692_002767"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7265043.1"
                     /translation="LCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAALAR
                     EAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIVAV
                     DIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWLAG
                     QETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAG
                     LVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQ
                     KVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIESDRLHCAK
                     RLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKLS
                     PYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSAP
                     "
BASE COUNT         3598 a         4603 c         3981 g         3808 t
ORIGIN      
        1 aaatcgccat aatcattcat tttaataatt aatgcgcttt cgccatttgt tacatcaata
       61 ttttgttcag gtacagtttg cagcgccgtc gccagcgcca acggattcca tgtcatatat
      121 tattccatat agattaagtt taaatattaa taaaatgaat atttgcaata cgtaattatc
      181 ttaccagcta tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagagg
      241 aactagctgc gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc
      301 agggaagaaa cacttcctgt cattgggatg ctgatcaact catcgcaatg ttcacgggtc
      361 aggcgacgca taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg
      421 ctctgataga gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc
      481 tgcaacatac gcatggtgcg cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc
      541 gcgccgcagg ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca
      601 atcaccgcat gaacgcctgc ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga
      661 tcggttacgc cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc
      721 agatcgtttt cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg
      781 gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga
      841 gactcaaggg cgtgaatcag cggtaacaga cgtttatcct cacggccttt taaaataaag
      901 acttcctgaa aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa
      961 atcatttcgc tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc
     1021 ggcctacatg atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt
     1081 tcacgccgca tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta
     1141 aagagggtat tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg
     1201 cggctatttt ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg
     1261 ctttcggctt cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt
     1321 taccgacctg acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc
     1381 gcgccgtttt accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt
     1441 tgcgctcgtc catattaacc gcttcgacgc gaacttccac gcgatcgccc aggcgataag
     1501 tctggccgct ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat
     1561 tgtccagcga agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga
     1621 agccaaagcc agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca
     1681 tgaagtcaca cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg
     1741 ccatcgaaca gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg
     1801 tttcagtggt gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt
     1861 gcagcgtcag gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg
     1921 ccagaccgaa gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca
     1981 gcatggtttg cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt
     2041 agtcacgcgg ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg
     2101 aacggaaaga ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt
     2161 ctttcgcttt ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa
     2221 ttaatttgtg cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt
     2281 tgaaaatgaa cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt
     2341 tatccagcac tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact
     2401 gctcgcgcag atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg
     2461 cgtgagagct catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa
     2521 ccgtcatctc gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt
     2581 tagagagcac ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac
     2641 ggttacgcgc ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa
     2701 tcgcgaccca taaacgccag ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa
     2761 agtcacgggc gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc
     2821 ccgcttttgc ttcttccggc acttcttctt tcagccccgc aacctgttgc tcaacagcct
     2881 gcggccagat atacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc
     2941 ccatattgtc gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac
     3001 gctgagtcag ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgattgggcg
     3061 ggattaagat atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt
     3121 cggtaaagta gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg
     3181 cttcacgacg acctttacgg tcagcaccca gcggctgcgc cagcacctga tcgccatgaa
     3241 tgcaggtttt catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca
     3301 gaaagccgta gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg
     3361 gcagcgcata gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca
     3421 ggcggcgacg caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct
     3481 catcacggct ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag
     3541 ggatgggatt cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg
     3601 ttcctccgtt gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag
     3661 cggttgattc tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact
     3721 ttgcacggcc ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc
     3781 actgctgcaa ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat
     3841 cgcactcgcc ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta
     3901 gccggcacga ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac
     3961 gtcagtcact tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag
     4021 cgcacgtaat ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc
     4081 gggaaaaaat acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt
     4141 cagttcgggg aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa
     4201 ccgacacagc aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca
     4261 gaattacgcg tcgaacgggt cgcgcagaat catggtttca gtacgatccg gaccggtaga
     4321 gatgatatcg atcggcacac cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc
     4381 ctgcggcagg ccgctacgat ctttcacgcc gaaagtggat tcagaccagc ccggcatggt
     4441 ttcgtaaatc ggctctacac ctttccagtc gtcagctgcc agcggagtgg tagtcacttc
     4501 gcgaccatcc ggcatacggt aagccacgca gagtttaacc tctttcaggc catccagaac
     4561 gtccagttta gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc
     4621 aacggtgtcc agccagccgg tacgacgacg acgacccgta gttgcaccga attcgttacc
     4681 ctgcttgcag aggaactcgc cagtttcatc aaacagttcg gccgggaaag gacctgcacc
     4741 tacacgagtt gagtaagctt tgaggatacc cagaacgtaa tcaacataac gcgggcccag
     4801 gccggaaccg gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt
     4861 accgtggtcg atatccagca gcgtaccctg cgcaccttcg aacatgacga aatcgccacg
     4921 ctgacgcgcc tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc
     4981 aacagccatc gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt
     5041 aaccaactgg aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt
     5101 gtcgaaaagg tcgccaacac gcagaccgcg acgtgccact ttatcttcat aagcaggccc
     5161 gataccacga ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc
     5221 cagcgcaacg tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg
     5281 aacggggatg ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag
     5341 cacaacaccg ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag
     5401 atggagaacg gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg
     5461 gtagcgtaca acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc
     5521 gtcaccccat tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt
     5581 gcttaaaaat ggattttacc atcgcttttt cagagttaca gcactttttg cactcaaaaa
     5641 tgacctcgat cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca
     5701 caacgcccgc aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg
     5761 tcatcgcaga gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca
     5821 gtaccaaaac cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa
     5881 aaagaaaacc accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt
     5941 tatattaacg cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc
     6001 tcatgaccat cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga
     6061 tgaatgcgta gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg
     6121 catcgccttc accacgcata atgcggccct gacgctcagc ttctgccagc gttctggtca
     6181 cttcatagtc ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac
     6241 gacgcgctac cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg
     6301 tcggcaggtt gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac
     6361 tgttcgggtt gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt
     6421 cggcaatggc gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg
     6481 agttcagcgc gtcacgtact tcgagggtca gacgaccacg ggaatcggtg acgatatctt
     6541 tcacgtccag gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca
     6601 cttccgcttg cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga
     6661 tgcaccattt gatgtaagag tcgacgatca ggtctttctt ctctttggtc acaaagcggt
     6721 cggcctggtt gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg
     6781 gtatcttgaa atgcagacca ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta
     6841 ccttaccaaa acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt
     6901 aaagcactac cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc
     6961 ctgacgctgg tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg
     7021 actggtggac gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg
     7081 cagcagattg ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat
     7141 ctggtctaac ggcagaacca tcaggttgcc acctttatcg ttaaccagca ctttgcgggt
     7201 gttacccaac actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc
     7261 ggctttatat tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat
     7321 ggtctgggcc ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg
     7381 cggctgaact tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg
     7441 cgcggcaatc gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa
     7501 gttgacgtcc agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg
     7561 ctgagtatcg ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt
     7621 accgataact ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt
     7681 cacgctatac agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg
     7741 cactacgttc tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc
     7801 cacgttcacc ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac
     7861 cagatggctg aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata
     7921 gaaaccactg gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc
     7981 gccaagctgc gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc
     8041 gccgcccaga ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg
     8101 cccttgatcg cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct
     8161 tccccacggg tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct
     8221 ccatatttgt tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc
     8281 ttcagacgct acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc
     8341 gacgccagtc aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc
     8401 actctttttc tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg
     8461 gcagacgcaa tgtatgctgc gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa
     8521 gctgtggtat ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt
     8581 cgtcccgatc aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca
     8641 gggttgggat ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt
     8701 cttgtacacg cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct
     8761 cttgtaacgt ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta
     8821 cggtatctgc aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt
     8881 cgagggtggc aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa
     8941 gggtagattt accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga
     9001 tacgcgattg ccgcccctgc tcacgctgct tttcaactct ttccaggcgc gactgtatct
     9061 gcacgatgcg attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac
     9121 gcaaacctat cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag
     9181 ccagatggcg cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt
     9241 gggcgaaaat atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct
     9301 ccaggttacg ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg
     9361 tcgctttgac agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt
     9421 gcggcgcttt acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca
     9481 gagattcaaa ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca
     9541 gtaccgcctg ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc
     9601 agcggggaac gcaggatcgc tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg
     9661 tttcttcgct gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac
     9721 tgctggtacc gccaccggcg ttgttactgt gatgagaaac cgggcgagac gggacaacag
     9781 tagaaatcgc gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact
     9841 gatcaaaaga ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg
     9901 gaacacgttc ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca
     9961 ttctctcttt tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc
    10021 gcacgatacg tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact
    10081 tcgtcacgcg cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc
    10141 agccaggtta tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt
    10201 tcatcgtatg agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga
    10261 atggaaggca agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca
    10321 aaacctgaag ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg
    10381 ctcgccgggg cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc
    10441 gtcagttccg ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt
    10501 tgtggatcat ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga
    10561 tgcaatgact cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg
    10621 tctgccgacg gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg
    10681 cccactaaca gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg
    10741 cggcgaaaat cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc
    10801 ggcgcggcga gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa
    10861 ataagggcag aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct
    10921 aacgccgttt taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc
    10981 ttactgatat cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa
    11041 cagaccaccc ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag
    11101 ggttatggcc tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca
    11161 ctgcgcaata ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg
    11221 aatcaagatt tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg
    11281 ctgtgcatct gactggaaat caatacccaa ttccgctagg gcagactgcg ctttttctaa
    11341 tgccgatttt tcttcgccag aaacttttag ccgcaacgga atcagcagcg gctgggcgca
    11401 aacgggcgct tcacccggcg tcaattgtgc ctgacgcagc caacgttctg ccactggcaa
    11461 ggataaaagt gaaatgttgc catcgcgctc cagcaacgca cagtcggaat ggacgatagt
    11521 cagtacccga ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc
    11581 ttttggtttt tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg
    11641 tttctggtag cctggctgcg cattcggcca gggggcagcc ggacgactgc ctgaggcggg
    11701 cgcaagagag tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg
    11761 ccccgccgcc acgcggtttt ccgggatggc acgcggtgcg ggttgtggtt catcgtccag
    11821 cggtagcggc gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc
    11881 atgcaccaga cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc
    11941 cacctgatgc ggggcgatct ccagatacaa cacaaatgcc ggttgctgat cggcccccag
    12001 tttgtcttcg caggcctggc ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc
    12061 attcacgtag caatactgaa tttccgccag tgcgggcgta gtgtgatttg gatcggccac
    12121 ccagccgcgc agggtgagat cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa
    12181 agcggtgccg caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg
    12241 gtactgacgc acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag
    12301 cgcaatgcgg cggatgattt catcaatatg gctaaattcg gttttctcgg tgcgcaggaa
    12361 tttgcgccgc gccggggtgt tgtagaacag atccagcacc tccagcgtcg ttcccaccgg
    12421 atgcgccgcc ggtttaaccg tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc
    12481 ttcctgctgt tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc
    12541 cagcgcctca ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat
    12601 tttactggtg gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc
    12661 gttatcacga atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt
    12721 cgcacctgca tcgaggctgt tttccactag ttctttgact accgacgcag gtcgctcgac
    12781 cacctcacct gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa
    12841 tcgtccttag tttggcagcg tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt
    12901 tgcaccctgc ggcgcagatt gcatcggatg cgcaaggaag taattgcgca ggcctttgta
    12961 aatggcttct gccagctgtt cttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt
    13021 gttgctgata aaaccggttt cgaccagtac tgatgggata tccggcgagc gcagaacgcc
    13081 aaggctggcg tgttctgatc gacgtttatg tatttcgcca atgcgttgca actgactgat
    13141 catactggtc gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac
    13201 cgcctggctt aaataggggt cagactgact gttcgccagc acatcacccg ccccacccag
    13261 cagctccgac tgtttctcgt gctgctccag ccagctggcc atttcactgt tggcgcgacg
    13321 gttagagagc acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg
    13381 aatcgacacg aggaaattgg cgttttgctt acgtgccaca tcactgcgcc ccatcaccga
    13441 gataaagtaa tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa
    13501 agtacgcaat ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc
    13561 gatagcgcca gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc
    13621 agtgttagcc gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt
    13681 agtgcggtta ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac
    13741 aaccgcaggc gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac
    13801 atcggcgtta atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc
    13861 ggttttaccg ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt
    13921 tccagagcga atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat
    13981 cacgcccgtt tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata
    14041 atcaggatcg ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg
    14101 aatatcagag agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa
    14161 ccaatttctg atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc
    14221 cagcaacaat tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg
    14281 ataatcaatg tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca
    14341 ctccaccagg cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc
    14401 ctcgggatcg gcaaggcggt acaaatcaaa gtgatagacc attaagttgt cgagcgtata
    14461 gggttcgacc agcgtataag tggggctttt gacattaccc tgatgaccca gagcctgtaa
    14521 aaagccccgg ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt
    14581 tgcgccatcg caggctttcg ctacccgctc gcccaggtct aatgttacct gctcatcagg
    14641 gagcggaatt actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct
    14701 gtagcgtgga aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta
    14761 cgtcagctgc cgcaccgtgc gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt
    14821 tttgcccaag caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca
    14881 tgcctgcatt tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc
    14941 cggcaccttt cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta
    15001 agcggtcact ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat
    15061 gcggcgtgat cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat
    15121 cggcatccca caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc
    15181 actcttgctg gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg
    15241 taagagagtc catagtcagt tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg
    15301 caatgttctc actgcgggtc agtactcgga ctaaaccagc accagcacgt agcgccgctt
    15361 cccccgtcat acgaatagcc cccgccgtgc cgtgatcgcc accgataatc accagccgcc
    15421 cgtgatcgcc tttatgcgaa gtcgggcgac gcggtttcag ccagtgagaa agttgttctg
    15481 ccgaaaaccg ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt
    15541 caaaatgcag ttgtcctgta acatcccgcg cttttccagt gagcaagcct agtttcagcg
    15601 caataaaagt gatggtgtga tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag
    15661 ccagcaggcc ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt
    15721 cgattaactg gctaatggat tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat
    15781 caacaatcag atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg
    15841 cgtttaacca tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct
    15901 gggccaacaa cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat
    15961 cgccgccgtt attaccatga ccgcacagca
//
LOCUS       DADLSE010000090        15739 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLSE010000090 DADLSE010000000
VERSION     DADLSE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.090"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            455..2734
                     /gene="ydeP"
                     /locus_tag="JN692_002768"
     CDS             455..2734
                     /gene="ydeP"
                     /locus_tag="JN692_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7265044.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2982..3179
                     /gene="safA"
                     /locus_tag="JN692_002769"
     CDS             2982..3179
                     /gene="safA"
                     /locus_tag="JN692_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7265045.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3253..4014
                     /gene="ydeO"
                     /locus_tag="JN692_002770"
     CDS             3253..4014
                     /gene="ydeO"
                     /locus_tag="JN692_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7265046.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            4416..6098
                     /locus_tag="JN692_002771"
     CDS             4416..6098
                     /locus_tag="JN692_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7265047.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6150..7307
                     /locus_tag="JN692_002772"
     CDS             6150..7307
                     /locus_tag="JN692_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7265048.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7273..7395)
                     /locus_tag="JN692_002773"
     CDS             complement(7273..7395)
                     /locus_tag="JN692_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265049.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            7604..9289
                     /locus_tag="JN692_002774"
     CDS             7604..9289
                     /locus_tag="JN692_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7265050.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGIWNLADDICDISTVL"
     gene            9327..11699
                     /locus_tag="JN692_002775"
     CDS             9327..11699
                     /locus_tag="JN692_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7265051.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANDSDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            11744..14539
                     /locus_tag="JN692_002776"
     CDS             11744..14539
                     /locus_tag="JN692_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7265052.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETF
                     ESMGLRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVITEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAADQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTVENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            14901..>15739
                     /locus_tag="JN692_002777"
     CDS             14901..>15739
                     /locus_tag="JN692_002777"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7265053.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLA"
BASE COUNT         4574 a         3467 c         3463 g         4235 t
ORIGIN      
        1 ggggacagta aataaaagca aagtacctct gggtttaagc gcaacctatg gacaaacagg
       61 aaacaaggtt tcagccggaa ccgtccagtc agtcattggt gtcactttta tctatgaata
      121 attgtgattt tgaccttagt ctatgcgctc cattcacgaa aattgcgcgg ataattattt
      181 tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag ccagagtctt
      241 attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc ttaacttatc
      301 gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta gtcccctact
      361 cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc gctattacaa
      421 atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg aatcctacca
      481 gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac gtaagcagat
      541 ggatatacgc catgatgtta ttgccatgtt tgacatgaat aagccagagg gctttgactg
      601 tccgggttgt gcatggccag atcctaagca cagtgcgtca ttcgacattt gtgaaaacgg
      661 cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt tctttgctga
      721 gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg cggggcgtct
      781 cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa gctggcaaca
      841 agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc aggttgaatt
      901 ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt ttgcccgtga
      961 atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga caagcgtggg
     1021 tttggcagcg agtatcggtg taggtaaagg gaccgtgttg ctggaagact ttgagaagtg
     1081 cgatttagtc atttgcattg ggcataaccc tggtacaaac caccctcgca tgctgacttc
     1141 gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctc tacaggaacg
     1201 tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga actctgagac
     1261 tcagttggcc agtgcctact acaacgtgcg cattggtggc gatatggcgt tgctcaaggg
     1321 gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc ggccctcatt
     1381 gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac gccgtgacgt
     1441 tctcaattcc gagtggaaag atatcgaacg tatttctgga ctaagtcaga cacaaatcgc
     1501 cgaactggct gacgcatatg ccgctgccga acgcaccatt atctgttacg gaatggggat
     1561 cactcagcac gaacatggta cccagaacgt gcagcaactg gtcaatttgc tgttgatgaa
     1621 aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgtggtcact ctaatgtaca
     1681 gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg ctcgcctggg
     1741 tgagcgctat ggcttccccc cacctcatgc acctggacat gctgcaattg ccagcatgca
     1801 agcaatatgt acggggcagg ctcgagcatt gatctgcatg gggggcaact ttgcactggc
     1861 aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg cggtacacgt
     1921 agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata ttctgccggt
     1981 tctggggcgt agcgagattg atatgcaaaa aagcggtgcg caggcggtaa ccgttgagga
     2041 ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg taatgctgaa
     2101 atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga gcgtggtagc
     2161 ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag ctgtgctgcc
     2221 agagttcgcc gactataacc agcgcatccg tcatcccggt ggttttcacc tgataaatgc
     2281 agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta ccagcaaagg
     2341 gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga cagtacgcag
     2401 ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag gggtattcgg
     2461 tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg taaaaaacgg
     2521 cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac gccgcatgga
     2581 tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct attttccaga
     2641 atcgaatcac atgctaacac ttgataacca cgatccatta agtggcattc ctggctataa
     2701 aagtattccg gttgaattag aaccatcaaa ttaatgcctc atctcatttc ttctgctgtc
     2761 atccgcacag cagaagaatt cctcattgac tattattgcg caatttgctc acatggatta
     2821 aattaaacta catactataa gatataaact tctgcctaca gctgtaagaa actccgctca
     2881 gtactgaagc accagtccta tttcttcttt tctccagcct gttatattaa gcatactgat
     2941 taacgacttt taacgttatc cgctaaataa acatatttga aatgcatgcg accacagtga
     3001 aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgcaatt gtggttgtct
     3061 tattactgac acttacgttg atagccattt tttcggcaat tgatcagctg agtatttcag
     3121 aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg caagactgaa
     3181 aataaacagc aaattataaa tgtgaaatgt ttaaaaggta tcgataaaac tttattgttt
     3241 taaggagata aaatgtcgct cgtttgttct gttatattta ttcatcatgc tttcaacgct
     3301 aacattttag ataaagatta cgccttctct gacggcgaga tcctgatggt agataacgct
     3361 gttcgtacac attttgaacc ttatgagcgg cattttaaag aaatcggatt taatgaaaat
     3421 accattaaaa aatatctaca atgcactaac atccagacag tgacggtgcc tgttcctgcg
     3481 aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat tgcttatctc
     3541 aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc ttgcctgtct
     3601 atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct atccgtttcc
     3661 gggaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct gaaagatatt
     3721 tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca agagcaaacg
     3781 acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt gatacgcgta
     3841 gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc ttattttatt
     3901 tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga gtaccgttgt
     3961 caaaggcaca cgggtatgaa tacgggcaac acgatgagtg ctttagctat ttgattattt
     4021 gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc cattccctgc
     4081 cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc atcacatatt
     4141 tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca ttttgtcagt
     4201 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
     4261 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
     4321 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
     4381 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
     4441 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
     4501 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
     4561 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
     4621 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
     4681 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
     4741 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
     4801 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
     4861 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
     4921 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
     4981 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
     5041 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
     5101 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
     5161 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     5221 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     5281 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     5341 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     5401 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     5461 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     5521 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     5581 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     5641 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     5701 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     5761 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     5821 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     5881 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     5941 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     6001 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     6061 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     6121 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     6181 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     6241 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     6301 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     6361 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     6421 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     6481 ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac agacgcagta
     6541 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     6601 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     6661 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     6721 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     6781 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     6841 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     6901 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     6961 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     7021 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     7081 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     7141 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     7201 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     7261 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     7321 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     7381 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     7441 gggcattagc aatacccttg attatcaata gcataaccat aaccataacc actgaaaatt
     7501 aaaaatttct atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc
     7561 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     7621 tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     7681 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     7741 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     7801 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     7861 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     7921 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     7981 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     8041 actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     8101 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     8161 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     8221 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     8281 cgaagcgaca tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct
     8341 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     8401 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     8461 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     8521 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     8581 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     8641 tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc
     8701 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     8761 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     8821 aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc
     8881 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     8941 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     9001 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     9061 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     9121 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     9181 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     9241 tatctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     9301 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     9361 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
     9421 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
     9481 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
     9541 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
     9601 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
     9661 gtgcaactaa taacctgaac ccagcgaatg attccgatgc cagtagtgca accaatatta
     9721 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
     9781 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
     9841 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgtttggact
     9901 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa
     9961 gtacctattt ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggagc
    10021 tggctgataa ctttggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
    10081 cggattatgt ttcgaatgac ggcattgtcg ccggtcgggc acagtataaa aacgttatcg
    10141 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
    10201 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg
    10261 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
    10321 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
    10381 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acccgtggcg
    10441 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
    10501 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
    10561 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
    10621 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
    10681 cgctgtatat ggcggatcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
    10741 atgactatga caactatctg tcaaaccaca atatctctcc gcgctttatg acggaatggg
    10801 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
    10861 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
    10921 ataaaaccct gacgcgttat cagaatttga aaacgcctta taacgatgaa ctggcaatgg
    10981 gattgcagca aaaaatcgat aagaacgtta ttgcacgcgc aaactatgtt taccgtgaag
    11041 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
    11101 aatataacaa cgatggcaaa accaaaacgc attcgtttaa cctcagtttt gaactggccg
    11161 aacccctgca tatccgccag gtagatatta acccgcaaat tgtctttagc tatatcaaga
    11221 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
    11281 tggtttataa cggtaatctg gtctcttacg atagcgttcc agtggcagat tttaataacc
    11341 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
    11401 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
    11461 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
    11521 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
    11581 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
    11641 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
    11701 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
    11761 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
    11821 cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
    11881 tatgatttat ccgcatgctc atccaaagga tcaggtaaat ttatggctgc aaattcatac
    11941 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
    12001 gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
    12061 cctgcgtttt ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt
    12121 gagtttgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
    12181 gagtaatgcc gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga
    12241 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
    12301 gctggctaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
    12361 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
    12421 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
    12481 aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
    12541 ccacctgcgc tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta
    12601 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
    12661 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
    12721 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
    12781 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc
    12841 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
    12901 tacccgcctc acctggctga aaaatgcggc tgatcagcaa gctgaacgtg atttacgtat
    12961 gctgaccagt cgcctagcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
    13021 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
    13081 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
    13141 tgccgccaaa aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa
    13201 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
    13261 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
    13321 ttccaatggt gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
    13381 tattgccgta tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
    13441 gctggcaaat aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa
    13501 acgctggagt gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
    13561 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg
    13621 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
    13681 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac
    13741 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc
    13801 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
    13861 cattgtcggt aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc
    13921 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
    13981 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
    14041 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
    14101 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt
    14161 ttcttctcgc ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac
    14221 ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg
    14281 tttgactaag gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag
    14341 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat
    14401 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt
    14461 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttataccgg
    14521 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    14581 tattcgctat ttttatgtaa taattttata aatgcgttca aaataataac caagtactaa
    14641 tagtgatatt ttaatgtctg atttttacgt gataattcag gaggcacaga aagcgcataa
    14701 aaataacaac ataaaacacc ttgccaccac ccaagaattc tctattgcat tgttttttaa
    14761 tgaaaaaata tcattccaac aatatctcac gataaataac attaggattt tgttatttaa
    14821 acacgagtcc tttgcacttg cttactttat cgataaatcc tactttttta atgcgatcca
    14881 atcattttaa ggagtttaaa atggataaga agcaagtaac ggatttaagg tcggaactac
    14941 tcgattcacg ttttggtgcg aagtctattt ccactatcgc agaatcaaaa cgttttccgc
    15001 tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    15061 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccaca
    15121 agttgatgga tttatccatt aacaaaaact ggatcgacaa agaagaatat ccgcaatccg
    15181 cagccatcga cctgcgttgc gtaaacatgg ttgccgatct gtggcatgcg cctgcgccga
    15241 aaaatggtca ggccgttggc accaacacca ttggttcttc cgaggcctgt atgctcggcg
    15301 ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc tgcaggcaaa ccaacggata
    15361 aaccaaacct ggtgtgcggt ccggtgcaaa tctgctggca taaattcgcc cgctactggg
    15421 atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt gtttatggac ccgaaacgca
    15481 tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc gactttcggc gtgacctaca
    15541 ccggtaacta tgagttcccg caaccgctgc acgatgcact ggataaattc caggccgaca
    15601 ccggtatcga cattgacatg cacatcgacg ccgccagcgg tggctttctg gcaccgttcg
    15661 tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag
    15721 gccataaatt cggtctggc
//
LOCUS       DADLSE010000091        15648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLSE010000091 DADLSE010000000
VERSION     DADLSE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.091"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(95..202)
                     /gene="rprA"
                     /locus_tag="JN692_002778"
     ncRNA           complement(95..202)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN692_002778"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(388..1500)
                     /gene="ydiK"
                     /locus_tag="JN692_002779"
     CDS             complement(388..1500)
                     /gene="ydiK"
                     /locus_tag="JN692_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7265054.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1889..4945
                     /locus_tag="JN692_002780"
     CDS             1889..4945
                     /locus_tag="JN692_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7265055.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGIVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4942..5352
                     /gene="menI"
                     /locus_tag="JN692_002781"
     CDS             4942..5352
                     /gene="menI"
                     /locus_tag="JN692_002781"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7265056.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5452..5640
                     /locus_tag="JN692_002782"
     CDS             5452..5640
                     /locus_tag="JN692_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265057.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6188..6556
                     /gene="sufA"
                     /locus_tag="JN692_002783"
     CDS             6188..6556
                     /gene="sufA"
                     /locus_tag="JN692_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7265058.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6565..8052
                     /gene="sufB"
                     /locus_tag="JN692_002784"
     CDS             6565..8052
                     /gene="sufB"
                     /locus_tag="JN692_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7265059.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKSVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEQSVG"
     gene            8062..8808
                     /gene="sufC"
                     /locus_tag="JN692_002785"
     CDS             8062..8808
                     /gene="sufC"
                     /locus_tag="JN692_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7265060.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8783..10054
                     /gene="sufD"
                     /locus_tag="JN692_002786"
     CDS             8783..10054
                     /gene="sufD"
                     /locus_tag="JN692_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7265061.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTESLRDEGLKQQVLARIGQRLPGGAR"
     gene            10051..11271
                     /gene="sufS"
                     /locus_tag="JN692_002787"
     CDS             10051..11271
                     /gene="sufS"
                     /locus_tag="JN692_002787"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7265062.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11284..11700
                     /gene="sufE"
                     /locus_tag="JN692_002788"
     CDS             11284..11700
                     /gene="sufE"
                     /locus_tag="JN692_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7265063.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11849..12853
                     /gene="ldtE"
                     /locus_tag="JN692_002789"
     CDS             11849..12853
                     /gene="ldtE"
                     /locus_tag="JN692_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7265064.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12917..13153)
                     /locus_tag="JN692_002790"
     CDS             complement(12917..13153)
                     /locus_tag="JN692_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7265065.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13464..14876)
                     /gene="pykF"
                     /locus_tag="JN692_002791"
     CDS             complement(13464..14876)
                     /gene="pykF"
                     /locus_tag="JN692_002791"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7265066.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     NATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15433..15642
                     /gene="fumD"
                     /locus_tag="JN692_002792"
     CDS             15433..15642
                     /gene="fumD"
                     /locus_tag="JN692_002792"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HAY7265067.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3971 a         3770 c         4145 g         3762 t
ORIGIN      
        1 cattatcagc ctgctgacgg cttgaagaga gtcacagtat cttgtgcaac attattggaa
       61 agagtgaggg gcgaggtagc gaagcggaaa aatgttaaaa aaaagcccat cgtgggagat
      121 gggcaaagac tacacacagc aattcgttgt ttcactcagg ggatttccat gcttataaat
      181 caatatgttg atttataacc gtgagctaat agtaggcata cggacttttt gcgtcgatca
      241 gattcgtctc attagtttaa aaactgtaaa gaatttgcga caggtactac cagctgaatg
      301 gcggtaagca ggaaaatatg gggaagagca agcaaatatc aattagcggt gattaacaat
      361 caggcggcag agccgcctga tgagaaatta cttattcggt ttctcgattt cgccaagttc
      421 ttcgagaatt tcttccggtt ggtccgtcgg cggcgggact tcttccaccc acgcggcaaa
      481 cagacgccag gaaacggcta acagaaccgg accaataaac agaccgatca tcccgaaagc
      541 aatcaaacca ccaataacgc cagagaggat caggatcagc ggtaaatcgg cacccatgcg
      601 aattaacatt gggcggatga cgttatccag cgtgccaacc acaccgctcc acactaacaa
      661 taccgttccc caggtggtat cgccggtcca gtagagccag ataatcgccg gaatcagtac
      721 cggcaacggg ccaagctgga caaggcagga gaggatcatt aacaccgtta gcaacgttgc
      781 ataaggtacg ccggatacgg cgaggccgat accgccaagc actgcctgta ctaacgccgt
      841 caccaccaca cccagcgcca ccgcgcggat agcctgtgcc gccagcagca cggcggcatc
      901 tccgcgaacg cctgccagac gggttgcaaa atggcgaatg ccttgtgcca cctgttcacc
      961 gcgccagtac agcagggcac tgaagagaag catcagcgca caatgcacca taaagcgccc
     1021 gatatgcgcc gcctgcccaa cgaaccaggt ggtggtggtg ccaatataag ggcggacttt
     1081 cgccatgatc gccgtacccc ccatatccag caagttgtgc cagcctgcat acagcttcgc
     1141 gccaatcacc ggaatggtat taagccacgc cagatcgggt aacgtcatgt cgccgctgga
     1201 aatggcttta attagcgggc cgctgccgtc gacgatactg ttaaccagca aagcaatagg
     1261 gatgataaac accatcacta ataacagcgt catcaccaga acggcgagag agcggcggcc
     1321 aaacatgatc ttttgcaaac gtaacaatac cggccaggtg gcgataacca ccgtaccggc
     1381 ccatgcaaag ccgagaataa agggttgaac aatccacaga catgccacaa tcatgatggc
     1441 taaaaacagc accgaaagca gaatttgtgc gacatccctg ggctgacgaa catttaccat
     1501 aactactttt cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc
     1561 acctgcataa tgatgagcaa tttcagcggt ttttaacagg ccgattctgc ccttaattct
     1621 gatgggcgtg ttagaaaaaa atgtggtaca acaactcagg caacacgcaa acgatttact
     1681 cgtcgtattt caagttgcat gtgctgcggc tacgttcgtt caccccagtc acttactatt
     1741 gtaagctcct ggagttcact cgcttgcagc cttcctgcaa ctcgaaatcc atagagtaaa
     1801 taaacatcac ctttcagtgc gctattgcag ccgcctgaaa gaatagtgta tcgacctaca
     1861 tcacagaccg caggaaaggg tcaatataat gattccacag atttcccagg cacccggcgt
     1921 cgttcaactg gtgcttaatt ttttgcaaga gctggagcaa caaggtttta ccggcgatgc
     1981 ggcgacaagt tatgccgatc gtctgacaat gtcgaccgac aacagtattt accaacttct
     2041 ccccgatgcg gtggtatttc cgcgttcaac cgcagatgtg gcgctgatcg cccgtcttgc
     2101 cgcgcaggaa cgctattcat cgctgatctt taccccccgc ggcggcggca ccggcactaa
     2161 cggtcaggcg ctcaaccagg ggattattgt tgatatgtcc cgccatatga accgcatcat
     2221 cgaaattaac cctgaagagg gctgggtgcg cgttgaggcc ggggtgataa aagaccaact
     2281 caatcagtac ctgaaaccgt tcggctactt ttttgcgccg gaactttcga ccagcaaccg
     2341 ggcaacgctc ggtgggatga tcaataccga tgcatccggt cagggatcgc tggtctatgg
     2401 caaaacgtca gatcacgtac ttggcgtacg cgcggtgttg ttggggggcg atattctcga
     2461 tacgcaacct ttacccgtcg agctggcgga aacgctgggt aaatccaata ccacaatcgg
     2521 gcgaatttat aacacggttt atcaacgttg ccgtcagcaa cgccagttaa tcatcgacaa
     2581 cttccccaaa cttaaccgct ttcttaccgg ttacgatctg cgtcatgtct ttaacgatga
     2641 gatgaccgag ttcgacctga cgcgcattct gacgggttca gaagggacgc tggcctttat
     2701 taccgaagcg cggctggata ttacgcgctt gcctaaagtg cgccgtctgg tgaatgtcaa
     2761 atatgactct tttgactctg cgctgcgtaa cgcgccgttt atggttgagg cgcgggcgct
     2821 ttcggtagag acggtggact caaaagtgct gaatctggcg cgggaagata ttgtctggca
     2881 ttccgtcagc gagttgatta ccgatgtgcc tgacaaagag atgctcggac tgaacattgt
     2941 ggaatttgct ggtgatgatg aggcgctgat tgacgagcga gttaatgcac tctgtgtgcg
     3001 gcttgatgag ctgattgcca gccaacaggc aggtgtgatt ggctggcagg tgtgccgcga
     3061 gctggcgggg gttgaacgta tctatgcgat gcgcaaaaaa gccgttggtc tgcttggcaa
     3121 tgccaaaggt gccgctaagc caattccgtt tgctgaggat acctgcgtac cgccggaaca
     3181 tctggccgat tatattgctg aatttcgcgc gctgctcgac agccacggct taagctacgg
     3241 tatgttcggt cacgtcgacg caggtgtctt gcacgtccgt ccggcactgg atatgtgcga
     3301 tccgcaacaa gagattttga tgaagcaaat ctctgatgac gtggtggcgc tgactgcgaa
     3361 atacggtggt ttgttgtggg gcgagcacgg caaaggtttt cgcgctgaat acagcccggc
     3421 gtttttcggt gaggaacttt ttgcagaact gcgcaaagtg aaagcggcat ttgacccgca
     3481 taaccgactc aacccaggga agatttgccc gccagaaggt ctcgatgcgc cgatgatgaa
     3541 agtggacgcg gtgaagcgcg gtacattcga tcggcagatc cccattgcgg tacgccagca
     3601 gtggcgcggt gcgatggagt gtaacggcaa cggtttatgc ttcaactttg atgcccgtag
     3661 tccgatgtgt ccgtcgatga agatcaccca gaaccggatt cattcaccga aagggcgcgc
     3721 aacgctggtg cgtgaatggc tgcgcttgtt ggcggatcgc ggcgttgacc cactcaaact
     3781 ggaacaagaa ctgcctgaat ccggcgtcag tttgcggacg ttaattgccc gcacgcgcaa
     3841 tagctggcat gcgaataaag gcgaatatga cttctcacac gaagtcaaag aggcgatgtc
     3901 gggctgtctg gcctgtaaag cttgttcgac ccagtgcccc atcaaaattg atgtgccgga
     3961 gtttcgctct cgttttctgc agctctatca cacccgttat ttacgcccgc ttcgcgacca
     4021 cctcgttgct acggtcgaga gctacgcacc gctgatggca cgcgcgccga aaacctttaa
     4081 cttcttcatt aaccagccgc tggtgcgcaa actctcggaa aaacatatcg gcatcgttga
     4141 tttgccactg ctgtcggtcc cctcgctaca acaacaaatg gtggggcatc gctcggcaaa
     4201 catgacgctg gaacagcttg aagctctcaa tgcagagcag aaagcgcgca cggtgttggt
     4261 ggtgcaggac ccctttacca gctattacga tgcgcaagtg gtggcggatt ttgtccgtct
     4321 ggttgaaaaa ttaggtttcc agcctgtgtt actgccattt tcgccaaatg gcaaagccca
     4381 gcatatcaaa ggctttctta atcgttttgc gaagacggcg aaaaagacgg cggatttcct
     4441 caaccgtatg gctaagcttg ggatgccaat ggtgggcgtc gatccggcgc tggtactttg
     4501 ttatcgcgat gaatataaac tggcgctggg cgaggaacgt ggcgagttta acgtcttact
     4561 tgcgaatgaa tggctggcaa gcgcacttga gtcacatcca gtggctacag tcagcggtga
     4621 atcatggtat ttctttggtc actgtaccga ggttaccgct ttgccgggcg cgccagcaca
     4681 atgggccgcg atatttgccc gttttggcgc gaaactggaa aatgtcagcg tgggttgctg
     4741 cggcatggca gggacttacg gacatgaagc gaaaaaccat gaaaattcgc tcgggatcta
     4801 tgagttatcc tggcatcagg ctatgcagcg actgccgcgt aaccgctgtc tggcgaccgg
     4861 atattcctgc cgtagccagg taaaacgggt tgaaggcacg ggggtacgcc atcctgtgca
     4921 ggctttactg gagattatta aatgatatgg aaacggaaaa tcaccctgga agcactgaat
     4981 gctatgggtg aaggaaacat ggtggggttc ctggatattc gctttgaaca tattggtgat
     5041 gacacccttg aagcgacaat gccagtagac tcgcggacaa agcagccttt cgggttgctg
     5101 catggaggag catccgtggt actggccgaa agtatcggtt ccgttgccgg ttatttatgt
     5161 accgaaggtg agcaaaaagt ggttggtctg gaaatcaatg ctaatcacgt ccgctcggca
     5221 cgagaagggc gggtgcgcgg catatgtaaa ccgttgcatc tcggttcgcg tcaccaggtc
     5281 tggcagattg aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc
     5341 gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg
     5401 cacagtaaac ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact
     5461 caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca
     5521 cctgcccaat atctcaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact
     5581 ctctcttctg ctgaattaaa agaagaaatc tactttgcct ggcgtctggg cgtgcattga
     5641 tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga
     5701 tgaccttctt aatactgctt atctaccgct tgatattgtt caaaaataat actaatcgca
     5761 gtttgtgtta aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct
     5821 aagccactga gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc
     5881 gcacagactt aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat
     5941 agtcattttc aatacaactt tttctttttc atcacagcat taatgctcct tagataataa
     6001 ccattatcta acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggg
     6061 ctatgcttat taaaagtagg caaaaagcat gttttacggt aaagcccctg cgtttgctgg
     6121 gttgaactga taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg
     6181 taaatagatg gacatgcatt caggaacctt taacccacag gatttcgcct ggcaaggctt
     6241 aacgctgaca cccgcagcgg cgatacacat ccgcgagctg gtggcaaagc agccaggtat
     6301 ggtcggcgtg cgcttaggtg tgaagcaaac gggctgcgcg ggctttggct atgtgctcga
     6361 cagtgttagc gagccggaca aagacgatct gctgtttgaa cacgacggcg cgaagctgtt
     6421 tgtcccgctg caatcgatgc cgtttattga tggcacggaa gtcgatttcg ttcgtgaagg
     6481 acttaatcag atattcaaat ttcacaaccc taaagcccag aatgaatgtg gctgtggcga
     6541 aagctttggg gtataggcgg tactatgtct cgtaatactg aagcaactga cgatgtcaaa
     6601 acctggaccg gcggcccgct gaattataaa gaaggattct tcacccagtt agccaccgat
     6661 gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa tttcggcgaa gcgtaatgag
     6721 ccggagtgga tgctggagtt tcgtctaaac gcctatcgcg catggctgga gatggaagaa
     6781 ccgcactggt tgaaagcgca ctacgacaag ctgaattatc aggattacag ctactactca
     6841 gcaccatcgt gcggtaattg tgacgacact tgcgcgtctg aacctggcgc ggtgcagcaa
     6901 actggcgcga acgccttttt aagtaaagag gtggaggcgg cgtttgagca gttgggcgtt
     6961 cccgtgcggg aaggcaaaga ggtggcggtg gatgccattt tcgactctgt ttcggttgcc
     7021 accacttatc gcgaaaaact ggcggagcag ggaattattt tctgttcctt tggtgaggcg
     7081 atccacgatc acccggaact ggtgcgtaaa tatctcggca ccgtggtgcc ggggaatgac
     7141 aacttctttg cagcacttaa tgcggcggta gcctctgacg gtacgtttat ttatgtgcct
     7201 aaaagcgtgc gctgcccgat ggaactttcc acctattttc gcattaacgc ggaaaaaacc
     7261 gggcagtttg agcgcacaat tctggtggcc gacgaagaca gctacgtcag ctacattgaa
     7321 ggctgttccg ctccggtgcg tgacagctat cagttgcacg cggcagtggt ggaagtcatc
     7381 atccataaaa acgccgaggt gaaatattcc acggtacaaa actggttccc tggcgataac
     7441 aacaccggcg gtattctcaa cttcgtcacc aagcgtgctt tgtgcgaagg cgaaaacagc
     7501 aaaatgtcat ggacgcaatc agaaaccggg tcagcgatta cgtggaaata tcccagctgc
     7561 attttgcgcg gcgataactc cattggtgag ttttactcag tggcgctgac cagcggtcat
     7621 cagcaagcgg ataccggcac caagatgatc cacatcggta aaaacaccaa atcgaccatt
     7681 atctcgaaag ggatctctgc cggacatagt cagaacagtt atcgcggctt agtgaaaatc
     7741 atgccgacgg caaccaatgc gcgcaatttc actcagtgcg actcaatgct gattggcgct
     7801 aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca ataacagcgc acaactggag
     7861 cacgaggcaa cgacatcacg tattggtgaa gatcaactgt tttactgcct gcaacgcggg
     7921 atcagcgaag aagacgccat ctcgatgatt gttaacggtt tctgcaaaga cgtgttctca
     7981 gaactgccgc tggaatttgc cgttgaagca caaaaactcc tcgccatcag tcttgaacaa
     8041 agcgtcggat aaggaataaa catgttaagt attaaagatt tacacgtcag cgtggaagat
     8101 aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg gcgaagttca cgccattatg
     8161 gggccaaacg gttcgggcaa aagtacctta tcggcaacgc ttgccgggcg cgaagattat
     8221 gaagtgacgg gcggcacggt tgagttcaaa ggcaaagatt tgcttgcact gtcgccggaa
     8281 gatcgtgcag gcgaaggcat ctttatggcc ttccagtatc cggtggagat tccaggcgtc
     8341 agtaaccagt ttttcctgca aacggcactt aatgcagtgc gcagctatcg cgggcaggaa
     8401 acgctcgacc gctttgattt tcaggatttg atggaagaga aaatcgctct cctgaagatg
     8461 ccggaagatt tattaacccg ttcggtaaac gttggttttt ccggcggcga gaaaaagcgc
     8521 aacgatattt tgcaaatggc ggtgctggaa ccggagttat gcattcttga tgagtcggac
     8581 tccgggctgg atattgacgc attaaaagtg gtcgccgatg gcgtgaactc gctgcgtgat
     8641 ggcaagcgct cattcatcat tgttacgcac taccaacgca ttctcgacta catcaagcct
     8701 gattacgttc atgtgctata tcagggacga attgtgaaat ccggcgattt cacgttggtc
     8761 aaacaactgg aggagcaggg ttatggctgg cttaccgaac agcagtaatg cgctgcaaca
     8821 gtggcatcac ttgtttgaag ctgaagggac aaaacgctca ccgcaagcac agcagcattt
     8881 acaacaattg ctgcgtaccg gactgccgac acgtaaacat gaaaactgga aatatacgcc
     8941 gctggaaggg ctgaccaata gtcagtttgt cagcattgcg ggagatatat ccccacagca
     9001 gcgtgatgcc ttagcgttaa cgttagacgc cgtgcggctg gtgtttgtcg atggacgtta
     9061 cgtgcccgcg ctgagcgatg caattgaagg cagcggatat gaagtgagca ttaacgacga
     9121 ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt ctgcatttga cggaaagcct
     9181 ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa cggccggcaa agccattgct
     9241 gttaatgcat atcacccagg gcgtggcagg tgaagaggtg aacactgccc attaccggca
     9301 tcatctggat ctggcggaag gtgccgaagc aacggtgatc gaacattttg tcagccttaa
     9361 tgatgctcgt cattttaccg gggcacggtt cactatcaat gtcgcagcga acgcccactt
     9421 gcagcatatc aagctggcgt ttgaaaaccc ggtcagtcac cactttgccc ataacgattt
     9481 gttgctggct gacgatgcca ccgcatttag ccatagtttc ctgctgggtg gcgcagtgtt
     9541 acgacacaac accagtacgc aactcaatgg cgaaaacagc acgctgcgga tcaatagcct
     9601 ggcgatgccg gtgaaaaacg aggtgtgtga tacccgtacc tggctggaac acaataaagg
     9661 tttttgtaac agccgacagt tgcacaaaac tatcgtcagc gacaaaggcc gcgcggtatt
     9721 taacggtttg atcaacgtcg cgcagcacgc catcaaaacg gatggtcaga tgaccaacaa
     9781 taatctgctg atgggtaaac tggcggaagt ggatacaaaa ccgcagctgg aaatctatgc
     9841 agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt attgatgatg aacagatgtt
     9901 ctatctgcgc tcgcgcggga tcaatcagca ggatgcccag cagatgatca tttacgcctt
     9961 tgctgccgaa ctgacggaat cactgcgtga tgaggggctt aaacagcagg tgctggcccg
    10021 aatcggtcaa cggctgccag gaggtgcaag atgacttttt ccgtcgacaa agtgcgggcc
    10081 gactttccgg tgctttcgcg tgaggtaaac ggtttgccgc tggcttatct cgacagcgcc
    10141 gccagtgcac agaaaccgag ccaggtgatt gacgccgagg ccgagtttta tcgtcatggc
    10201 tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc aggcgaccga gaaaatggag
    10261 aacgtgcgca agcgggcatc gctgtttatt aatgcccgtt cggcggaaga gctggtgttc
    10321 gtccgcggca cgacggaagg gatcaatctg gtcgccaata gctggggcaa cagcaatgtg
    10381 cgggcgggcg ataacatcat catcagtcag atggagcacc acgctaacat tgtcccctgg
    10441 cagatgcttt gcgcacgcgt tggcgcagag ctgcgtgtga tccctctcaa ccccgacggt
    10501 acgctgcaac tggagacgct gcctacgctg tttgatgaga aaactcgcct gctggcaatt
    10561 actcatgtct ccaacgtgct tggcacagaa aatccactgg cggaaatgat cacgcttgcg
    10621 caccagcatg gcgcaaaagt gctggtggat ggcgctcagg cggtgatgca tcatccggtg
    10681 gatgttcagg cgctggattg cgacttttac gtgttctccg ggcataaact gtatggcccc
    10741 accggaattg gcattcttta tgtgaaagaa gccttgttgc aggagatgcc gccgtgggaa
    10801 gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag gcactacctg gaccaaagca
    10861 ccatggcggt ttgaagccgg tacacccaat accgggggca tcattggtct tggcgcggcg
    10921 ctggagtatg tttcggcgct ggggcttaat agcatagccg agtatgaaca gaatctgatg
    10981 cattatgcgc tatcacagct ggaatctgta ccggatctca ctctctatgg cccacaaaac
    11041 aggcttggcg ttattgcttt taatctcggt aaacaccacg cctatgatgt tggcagtttt
    11101 ctcgataatt acggcattgc tgtgcgtacc ggacatcact gcgctatgcc attaatggcc
    11161 tattacaacg tccctgcgat gtgtcgggcg tcgctggcca tgtataacac ccatgaagaa
    11221 gtggatcgtc tggtgaccgg cctgcaacgt attcaccgtc tgctgggata acagggaggc
    11281 actatggctt tattgccgga taaagaaaaa ttgctgcgta attttttacg ctgcgccaac
    11341 tgggaagaga aatatctcta cattattgag ctgggccagc gtctgccaga attacgcgcc
    11401 gaagaccgaa gcccacaaaa tagcattcag ggctgccaga gtcaggtgtg gattgtcatg
    11461 cgccagaatg cgcagggaat tattgaattg cagggcgaca gcgatgcggc gattgtgaaa
    11521 gggcttattg cggtcgtctt tattctctac gatcagatga cgccgcagga tattgtcaat
    11581 ttcgatgtgc gtccgtggtt tgaaaaaatg gcgctcaccc aacatctcac cccatctcgt
    11641 tcacaaggtc tggaagcgat gattcgcgca attcgcgcca aagccgctgc acttagctaa
    11701 actaaaggga cagctttcat ccggttggct cacattgccg gatggtcaat gtttaaactg
    11761 acctgcacag gagtgttttg gtttcaggtg agcataagcg ccatccgacg tttcagcgtg
    11821 agtctccggc aaatacagga ggtttacaat gaaacgcgcg tctttgctta cactcacgct
    11881 tatcggcgct tttagcgcca tccaggctgc ctgggcggtt gattatccgc taccaccaac
    11941 cggaagccga ctggttgggc aaaatcaaac gtatacggtg caagaagggg ataaaaacct
    12001 tcaggctatt gcccgacgtt ttgatactgc ggcaatgttg atccttgaag ccaataacac
    12061 tatcgccccg gtgccaaaac ctggtacgac gataactatt ccttcacaac tgttattacc
    12121 tgatgcaccg cgtcagggga ttatcgttaa ccttgcagag ctgcgccttt attattatcc
    12181 gccgggagaa aatattgtgc aggtttatcc aataggtatt ggattgcagg ggctggaaac
    12241 gccggtgatg gaaacgcgtg ttgggcagaa aatccctaac ccaacctgga cgcctacggc
    12301 aggcattcgt cagcgttcgc tggagcgtgg cattaaatta ccgccagtcg ttcctgccgg
    12361 accaaataac ccgctaggac gttacgcact gcgcctcgcg catggtaatg gcgaatacct
    12421 cattcatggc accagtgcgc cggacagcgt tggtttgcgc gtcagttcag ggtgtattcg
    12481 catgaatgca ccggatatta aagccttgtt ctccagcgtg cggacgggaa cgccggtgaa
    12541 agtgatcaac gaaccggtga aatattccgt ggaacctaac gggatgcgtt atgttgaagt
    12601 acatcgacca ctatcagcag aagaacagca aaacgttcag acaatgccat acacactgcc
    12661 agcaggcttt acgcaattta aagacaataa ggctgtagat cagaagttag tcgataaagc
    12721 gttgtatcgt cgggcagggt atccggtttc ggtgagcagt ggagcaactc ccgcagccag
    12781 caatgcgcct tcagtagagt cagcgcagaa tggtgaacca gagcaaggga atatgttacg
    12841 cgcgacgcag tagtggtaaa tagcagacaa aaaaaatggc gcacaatgtg cgccattttt
    12901 cacttcacag gtactattac ttgcggtatt tagtagccat gttgtccaga cgctggttag
    12961 cacgagctgc gtcatcttta gcagcctgaa cgtcggaacg cattgcgttc acgtcgttgc
    13021 tcagctggtc aactttagcg ttcagagtct gaacgtcaga agacagctga tcgattttag
    13081 cgttgctgga gcaacctgcc agcagagtag aacccaggat taccgcgccc agtaccagtt
    13141 tagtagcttt cattattaat accctctaga ttgagttaat ctccatgtag cgttacaagt
    13201 attacacaaa gttttttatg ttgagaatat ttttttgatg ggaaggcact tatttttgaa
    13261 cgttcgctca aagaagcatc gaacgctggg ttttagataa aaaaattgag agaaaacagg
    13321 atgcttccat cggattcatc ttagataaag tgagattata tagtgaaatc cgatttgatt
    13381 ttttattgct tctggttatc gattaaataa aaaaagcgcc catcagggcg cttcgatata
    13441 caaattaatt cacaaaagca atattacagg acgtgaacag atgcggtgtt agtagtgccg
    13501 ctcggtacca gtgcaccaga aaccataact acgacgtcac ctttgtgtgc cagaccgctc
    13561 tgcagagcca gttctttacc cagacggtag aaatcatcag tagaagtgat ctctttaacc
    13621 agctgcggca caacgccttt gctcagtacc aactgatgag ccgttttttc gttggtggtc
    13681 agtgccagga tggtggcatt cgggaagtat ttacgtactg cgcgagcaga tttaccgccc
    13741 tgggtagcaa ccacgatcag cggagcatcc agtttttcag cagtttcaac ggcaccacgg
    13801 cataccgctt cggtgatgcg cagtttacgg ttgtcattgt tgaactcgag acggctgttc
    13861 atcacgcggt cggtacgttc gcagatggtc gccatgatag aaaccgcttc cagcgggtat
    13921 ttaccttttg cggattcacc agacagcatc actgcgtcag taccgtcgag gatggcgttt
    13981 gcaacgtcac cggcttctgc gcgagtcggg cgtgggtttt tgatcatgga atccagcatc
    14041 tgggtcgcag tgataacgac tttacgtgca cggatacatt tttcgatcat catcttctgg
    14101 gcgaagataa cttcttctac cgggatttct acacctaggt cgccacgcgc aaccatgatg
    14161 ccgtcagagg cttcgaggat ttcgtcgaag ttgttgaggc cttcctggtt ttcgattttg
    14221 gagatgatgt ggatgttttc gccgccgtgc gctttcaggt gctcacggat ttcgataacg
    14281 tcagaacgct tacgaataaa ggaagcagca acaaagtcta cgcctttttc gcaaccaaag
    14341 atcaggtcct gtttgtcttt ttcagccagt gctggcagag caatggaaac gccaggcagg
    14401 ttcacacctt tgttttcgcc caggtcaccg ttgttcagca ctttacagat aactttgtta
    14461 ccttcaatgg cggtaacttc cataccgatc agaccatcgt caaccagtac ggtgttgcca
    14521 acagacaggt cagtagtgaa accttcatac gttaccgcaa ccatttcgct gttgccgata
    14581 acagatttat cagtggtgaa agtaaaggtc tgaccagctt tcagagaaac atcgttaccg
    14641 ccttccagtt tcatggtgcg gatttccgga cctttggtat caagcaggat agcggcggtt
    14701 ttaccagttt tgctcatcac gttgcgcaga ttctgaatgc gctgaccgtg ttctgcatag
    14761 tcaccatgag agaagttcag acgcataacg ttcatgccag cgtccagcat tttagctaac
    14821 atctcttcag attcggtttt cggtccgatg gtgcaaacaa ttttggtctt tttcatgaca
    14881 gtcttagtct ttaagttgag aaggatggga gaaacttgct ttctgggcgc actgccggaa
    14941 agcgaatccg gtattacgaa agtggtggtg cctcgctcta aggataggtg acatcgaaaa
    15001 agcgtgcaga ggaatgtgtg cttgtgtctc agcccaacgt gagtgcctgt ttttgagtcg
    15061 ttgagttcta cagtccaaag tgctgaaacc attcaagagt caattggcgc gcattatacg
    15121 ctgaaattaa tccaaaagga aaacaaaaac agcgtttcaa aaaatataca acttgttaga
    15181 caataaattg ttatcaaaga tttaacgttc cagggaaaag gttacgcaaa aacttcgctg
    15241 tttgcgcaac gtctggtgcc gaagttgtca gtgtgaggat aagtgaaagt tcatccggtg
    15301 tataaatata tcatcgctca attgctacag ttaaaataat ttatcgcgtt atttgtctga
    15361 ttttgtttga tttttgttta attggttaga ttgatatatg ctgatagttg gcatgataaa
    15421 taggtataaa gaatgggaaa tcggacaaaa gaggatgaac tgtaccgaga aatgtgcaga
    15481 gttgtcggta aagttgtgct ggaaatgcgc gatctggggc aggaaccaaa gcatattgtt
    15541 atcgcaggcg tattacgtac agcattagcc aataaacgca ttcagcgcag tgagctggaa
    15601 aagcaggcga tggaaaccgt cattaacgca ctggtaaaat gaccattc
//
LOCUS       DADLSE010000092        15228 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLSE010000092 DADLSE010000000
VERSION     DADLSE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.092"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            215..1516
                     /gene="rstB"
                     /locus_tag="JN692_002793"
     CDS             215..1516
                     /gene="rstB"
                     /locus_tag="JN692_002793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7265068.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            1592..2521
                     /gene="tus"
                     /locus_tag="JN692_002794"
     CDS             1592..2521
                     /gene="tus"
                     /locus_tag="JN692_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7265069.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2518..3921)
                     /gene="fumC"
                     /locus_tag="JN692_002795"
     CDS             complement(2518..3921)
                     /gene="fumC"
                     /locus_tag="JN692_002795"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7265070.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(4064..5710)
                     /locus_tag="JN692_002796"
     CDS             complement(4064..5710)
                     /locus_tag="JN692_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7265071.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5909..7084
                     /gene="manA"
                     /locus_tag="JN692_002797"
     CDS             5909..7084
                     /gene="manA"
                     /locus_tag="JN692_002797"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7265072.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7185..8693
                     /locus_tag="JN692_002798"
     CDS             7185..8693
                     /locus_tag="JN692_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7265073.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8919..10184)
                     /gene="uidC"
                     /locus_tag="JN692_002799"
                     /pseudo
     CDS             complement(8919..10184)
                     /gene="uidC"
                     /locus_tag="JN692_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
     gene            complement(10223..10390)
                     /locus_tag="JN692_002800"
     CDS             complement(10223..10390)
                     /locus_tag="JN692_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HAY7265074.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(10406..11402)
                     /gene="uidB"
                     /locus_tag="JN692_002801"
                     /pseudo
     CDS             complement(10406..11402)
                     /gene="uidB"
                     /locus_tag="JN692_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11399..13210)
                     /gene="uidA"
                     /locus_tag="JN692_002802"
     CDS             complement(11399..13210)
                     /gene="uidA"
                     /locus_tag="JN692_002802"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7265075.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLDIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13598..14188)
                     /locus_tag="JN692_002803"
     CDS             complement(13598..14188)
                     /locus_tag="JN692_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7265076.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14417..15184)
                     /gene="hdhA"
                     /locus_tag="JN692_002804"
     CDS             complement(14417..15184)
                     /gene="hdhA"
                     /locus_tag="JN692_002804"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7265077.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         3841 a         3939 c         3604 g         3844 t
ORIGIN      
        1 gagactaaag aacaacaaag cgtcacgtta aattaaacct tagcataata aacaagcccc
       61 ggtggaaaat tgaatttact gaatatgaat acgccgggga tacatccgtc tgttccggac
      121 attatcttcc aaacaaggtc ttatccagtt ggcggtttac attgctccac agtcgtgaat
      181 taatacgaat agtattctgt tgcgggcgat gccaatgaaa aaattgttta tccagtttta
      241 cctgttattg tttgtctgct tccttgtgat gtctctgctg gttgggctgg tgtacaaatt
      301 taccgccgaa cgcgcgggca aacagtcgct ggatgatttg atgaacagtt cgctgtatct
      361 gatgcgcagc gaattgcgag agatcccccc acacgactgg ggtaagacgc tgaaagagat
      421 ggatttaaat ctctctttcg atctgcgtgt cgagccactg agtaaatacc atcttgatga
      481 tatttccatg caccgactgc gtggcggcga aattgtcgcc ctggacgatc agtacacgtt
      541 tttgcagcgt atcccgcgca gccactacgt gctggcagtt ggtcctgttc cttatcttta
      601 ttacctccat caaatgcgat tgctggatat cgccctgatc gcttttattg ctatttccct
      661 cgcctttccg gtgtttatct ggatgcgtcc gcactggcag gatatgttaa aactggaagc
      721 agcggcgcaa cgatttggcg atgggcatct caatgaacgt atccactttg atgagggttc
      781 gagctttgaa cgacttggta tcgcatttaa ccagatggcg gacaatatca acgccttaat
      841 tgccagcaaa aaacagctta ttgacggtat cgctcacgaa ctgcgaacac cgttagtgcg
      901 cctgcgttat cgactggaga tgagcgataa cctgagcgcc gccgaatccc aggcgttgaa
      961 tcgtgatatc agtcaacttg aagctttaat tgaagagctg ctgacttatg cccgactcga
     1021 tcgcccacaa aacgagcttc atcttagcga accagacctg ccgttgtggc tgtcaacgca
     1081 tctggcagat attcaggcag taacgcccga taaaacggta cggataaaaa cgctcatgca
     1141 aggccattat gcggcgttgg atatgcgctt aatggagcgc gtgctggata atttgctcaa
     1201 taacgccctg cgctactgcc attcaacggt tgaaaccagc ctgctactgt cggggaatag
     1261 agcgacatta attgttgagg atgatggccc agggattgcc ccagaaaacc gcgaacatat
     1321 ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc tcaaccggcg gctgcgggct
     1381 ggggctggca attgtccact ctatagcact ggcaatgggc ggtacggtta attgtgacac
     1441 cagcgaactg ggtggtgctc gcttctcgtt tagctggccg ttatggcata acatcccgca
     1501 atttacctct gcctgacact acgcgcacga tggtcaagtc accacgactg tgctataaaa
     1561 taagtatgtt gtaactaaag tggttaatat tatggcgcgt tacgatctcg tagaccgact
     1621 caacactacc tttcgccaga tggaacaaga gctggctaca tttgccgctc atcttgagca
     1681 acacaagcta ttggttgccc gcgtgttctc tttgccggag gtaaaaaaag aggatgagca
     1741 taatccgctt aatcgtattg aggtaaaaca acatctcggc aacgacgcgc agtcgctggc
     1801 gttgcgtcat ttccgccatt tatttattca acaacagtcc gaaaatcgca gcagcaaggc
     1861 cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat aacctttcgc aagcagcgtt
     1921 ggtcagtcat attcagcaca tcaataaact caagaccacg ttcgagcata tcgtcacggt
     1981 tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg catcgtcatt tgccggggct
     2041 gatcaccctt aatgcttacc gcacgctcac cgttctgcac gaccccgcca ctttacgctt
     2101 tggttgggct aataaacata tcattaagaa tttgcatcgt gatgaagtcc tggcacagct
     2161 ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg acgcgcgagg agtggcaaag
     2221 aaaactggag cgagagtatc aggatatcgc tgccctgcca cagaacgcga agttaaaaat
     2281 caaacgtcca gtgaaggtgc agccgattgc ccgcgtctgg tacaaaggag atcaaaaaca
     2341 agtccaacac gcctgcccta caccactgat tgcactgatt aatcgggata atggcgcggg
     2401 cgtgccggac gttggtgagt tgttaaatta cgatgccgac aatgtgcagc accgttataa
     2461 acctcaggcg cagccgcttc gtttgatcat tccacggctg cacctgtatg ttgcagatta
     2521 acgcccggct ttcatactgc cgaccatctg ttctggccgt acccagctgt caaactcggc
     2581 ttcgctaaga taccccagcg caagggccgc agcttttaag gtcagccctt ctttatgcgc
     2641 ttttttggcg atctccgcgg ctttgtcata accaatgtgg gtgttaagcg cagtcaccag
     2701 catcagcgat tcattgagta attgattgat tcgctcacga ttcggttcaa tacccactgc
     2761 gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag cgcaccgatt gcaggaaatt
     2821 atggatcacc atcggacgga agacgttcag ttcaaagtta ccggaagcgc cacccatgtt
     2881 gatcgccacg tcgttcccca ttacctgaca gcagagcatg gttaatgctt cacactgggt
     2941 tgggttcact ttccccggca tgattgagct gcccggctca ttttccggga ttgagatttc
     3001 accaattccg cagcgcgggc cagaggccag ccagcggaca tcattggcga ttttcatcag
     3061 tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc agggcatcac aggtcgcaag
     3121 cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag gtaatgactg ccagttcatc
     3181 tgctacgcga cgcgcatact ccggatgggt atttagtcca gtacccaccg ctgtaccgcc
     3241 aagagccagt tccgctacgt gaggcaggct gtattcgata tgtttgagat tatgctcgag
     3301 catcgctacc cagccggaaa tctcctgccc tagtgttagc ggcgtggcgt cctgcaagtg
     3361 ggttcgaccg attttgacga tatcggcaaa tgcacgcgat ttttcactca gcgtctgtgt
     3421 cagggtttta agctgcggaa tgagttgctt gcgcagtgcc agcagcgccg caacgtgcat
     3481 cgccgtcgga aagacatcgt tggaactttg gcttttgttc acgtcgtcgt tagggtgaac
     3541 tttacgttcc atcccgcgca caccgccgag taattcactg gcccggttag ccagcacttc
     3601 gttcatgttc atgttacttt gcgtgccgga gccggtctgc cagatagcca gcgggaattc
     3661 gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc cgaatggcgc tcgctttctc
     3721 ttcagacaac aagtctaaat cttcattaac ttttgccgct gcgcgcttgg ttagcgccag
     3781 cgcatgaatc agtgatgtgg gcattttctc cgtcgaaatg cggaaatgct ccagcgagcg
     3841 ttgagtttgt gcgccccaca gcttatctgc cgggacatca atcgccccca tcgaatcttt
     3901 ttcgctgcgt actgtattca tggcctgctc ctcacctgat taattttttc tttctgtttt
     3961 gctttcgtta agcaactttt agctcactca ttatttacta tttgataaca aatgtttggt
     4021 ctttcgtgcc atgtaaaaaa accgccccga agggcggctc tgtttatttc acacagcgag
     4081 tgcactgtgt gagttgtatc tgctggaaga agtcatttcc tttatcatcc acaaggataa
     4141 acgccgggaa atcttccact tcaattttcc agatggcttc cattcccagt tccggatatt
     4201 caacacattc caggctctta atacttccct gcgccaatac agcggccgga ccaccgatac
     4261 tgccaaggta gaagccgccg tgttttttac aggcatccgt cacctgctgg ctgcggttgc
     4321 ctttcgccag catgatcata cttccgccct gcgcttgcag ttgatcgaca taagaatcca
     4381 tccgtccggc ggtcgttggg ccaagagaac cggaggcata accttccggc gttttggccg
     4441 gacccgcgta gtaaatcgga tgatctttaa tgtattgcgg cagcccttca ccgttatcca
     4501 tccgctcttt cagtttggcg tgagcaatat cacgaccaac gataatcgtg ccgttaagcg
     4561 ataagcgtgt agaaacggga tactgcgaca actgtgcgag gatctctttc atcggacggt
     4621 taaggtcaac gcgcaccgct tcgccttctc ccgctttacg cagctcttca gggatatatt
     4681 tacctggatt atgttccagt ttttcgatcc agatcccctg acggttgatc ttcgctttga
     4741 tattacggtc agcagagcag gaaacaccca taccaaccgg acaggatgcg ccgtgacgtg
     4801 gcaggcgaat cacgcggatg tcgtgagcga agtatttacc accaaactgc gcacccagac
     4861 cgagattttg cgcttcgatc agtaattctt tttccagttc cacatcgcgg aacgcctgac
     4921 cgtgctcatt cccttccgtt ggcagttcat catagtattt cgcggaagcc agtttcaccg
     4981 ttttaaggtt cgtttctgca gaagttccac caataacgaa cgcaatatga tacggaggac
     5041 aggccgcggt acccagcgtg cgcatcttct caaccaggta atttttcagt ttccccggcg
     5101 tcagtaacgc tttggtttcc tgatagagat acgtcttgtt tgccgaacca ccaccttttg
     5161 cgatacagag gaatttgtat tcgtcgccat caacggcata aagatcgatc tgcgctggca
     5221 gattggtgcc ggtattcact tctttataca tatccagcgg cgcgttttgc gagtagcgca
     5281 gattatcttc gatataagtg ttatagacac cgcgcgccag cgccgcttca tcaccaccac
     5341 cggtccatac acgctgccct tttttaccaa caataatcgc ggtgccggta tcctgacagg
     5401 ttggcagaac gcctttcgcc gcgatgtcgg agttacgcag gaattgcagc gccacatatt
     5461 tatcattttc gctggcctcc gggtcacgca gaatgtcggc cacctgttgt tggtgagccg
     5521 gacgcagcat aaacgacgca tcatgaaacg cctgacgcgc caacagagtt aacgcttcgg
     5581 gtgcgacttt caaaatctcc tgcccttcaa attcagatac gctaacgtgt tcactggtta
     5641 gcaggtaata ctcagtatca tcttttttga gtggaaaagg agactgataa tgaaagggtt
     5701 tgtttgacat tgttctctca cttactgcct ggtttggtta tgctctgggc gggtgttccg
     5761 ttgccctgtt aaaagcgagt aacaatatcc tacacagttt tttaacaaaa actgagacta
     5821 gtacgacttt ttgcggctcc aggttacttc ccgtaggatt cttgctttaa tagcgggatt
     5881 aatttccaca ttaaaacagg gattgatcat gcaaaaactc attaactcag tgcaaaacta
     5941 tgcctggggt agcaaaacgg cgttgactga actttatggt atggaaaatc cgtccagcca
     6001 gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacgag tgcagaatgc
     6061 cgccggagat atcgtttcac tgcgtgatgt gattgagagt gataaatcga ctctgctcgg
     6121 agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat tatgcgcagc
     6181 acagccactc tccattcagg ttcatccaaa caaacacaat tctgaaatcg gttttgccaa
     6241 agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag atcctaacca
     6301 caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt ttcgtgaatt
     6361 ttccgagatt gtctccctac tccagccggt cgccggtgca catccggcga ttgctcactt
     6421 tttacaacag ccacatgccg aacgtttaag cgaactgttc gccaacctgt tgaatatgca
     6481 gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gcgctcgaca gtcagcaggg
     6541 tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca gcggcctgtt
     6601 ctccccgctg ttactgaatg tggtgaaatt gaatcctggc gaagcgatgt tcctgttcgc
     6661 tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcga actccgataa
     6721 cgtgctgcgt gcaggtctga cgcctaaata cattgatatt ccggaactgg ttgccaatgt
     6781 gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac aaggtgcaga
     6841 actggacttc ccgattcctg tggatgattt tgccttctcg ctgcatgacc ttagtgataa
     6901 agaaaccacc atcagccagc agagtgccgc cattttgttc tgcgtcgaag gcgatgcaac
     6961 gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt ttattgccgc
     7021 caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt acaacaagct
     7081 gtaagagctt actgaaaaaa ttaacatctc ttgctaagct tattaaaggc ttataacacc
     7141 ttcaggcggc cagtccgcct gatttcattt tatggataat cattatgaat aaatcgctgg
     7201 tagcggtagg cgtcattgtt gcgctaggcg tagtctggac aggcggcgca tggtatacag
     7261 gcaagaagat tgaaacccat ctcgaagaca tggtcgcgca ggcgaacgcg caactcaaac
     7321 tgacagctcc tgaatccaac ctggaagtga gttatcaaaa ctatcatcgc ggcgtattca
     7381 gcagccagtt gcaactgttg gtgaaaccca ttgccgggaa agaaaatccg tggattaaaa
     7441 gcggtcagag cgtcatcttc aacgaatcgg ttgatcatgg tcccttcccg cttgcccagc
     7501 ttaaaaaact gaacctgatc ccgtcgatgg catcaattca aaccacgctg gttaataacg
     7561 aagtaagcaa accactgttt gatatggcaa aaggtgaaac gccttttgag attaactcgc
     7621 gcattggtta cagcggtgat tccagttccg atatttcgct caatccactg aattacgagc
     7681 aaaaggatga aaaagtcgcc tttagcggcg gcgagttcca gttaaatgct gacagagacg
     7741 gcaaagccat ctccctttcc ggggaggcgc aaagtggtcg gatagacgca gttaacgaat
     7801 acaaccagaa agtgcagttg acctttaata atctgaaaac cgacggttcc agcacgctgg
     7861 caagttttgg tgagcgcgta ggaaaccaaa aactgtcact ggaaaaaatg accatttcag
     7921 tggaaggcaa agaactggca ctgctggaag gcatggagat cagcggtaaa tcggatctgg
     7981 tcaatgacgg taaaacgatc aatagccaac tggattactc gctaaacagc ctgaaggtac
     8041 agaatcagga tctgggcagc ggcaagctga ctttaaaagt cggccagatt gatggtgaag
     8101 cctggcatca gtttagccag caatataacg cgcaaactca ggcgctgctg gcacagccag
     8161 aaattgccaa caatcccgaa ctttatcagg agaaagtgac ggaagccttc tttagcgccc
     8221 tgccgctgat gttgaaaggc gatccggtga ttactatcgc gccgctaagc tggaaaaaca
     8281 gtcagggtga aagtgcgctg aatctgtcgc tgttcctgaa agatccggca acgactaaag
     8341 aagcgccgca aacgctggcg caggaagtag atcgttcggt taaatctctg gatgcgaaac
     8401 tgaccattcc ggtggatatg gcaactgagt tgatgactca ggtagcgaag ctggaaggtt
     8461 atcaggaaga tcaagcgaaa aaactggcga aacagcaagt tgaaggtgca tcagcaatgg
     8521 ggcagatgtt ccgtctgacc accttgcagg acaataccat caccaccagc ctgcaatatg
     8581 ctaacggtca gataacgtta aatgggcaga aaatgtcgtt agaagatttt gttggcatgt
     8641 ttgcgatgcc agctcttaac gttccggctg taccggcaat tccgcagcag taattcacgg
     8701 ccaaaacgcc ggagaatttt cactccggcg ttttcatctg aaaacaaacc agagattgcc
     8761 tgatgcgcta cgcttatcag gcctaccttt tcctgcaaca ctttgaattt atgagttttt
     8821 gtaggctgga taaagcgttt acgccgcatc cggcataaaa aacgcgcact ttgtcaacaa
     8881 tctgaaacgc cggaggtttt tctctccggc gttatttctt acttcagcat aaagtcatac
     8941 tgaatactca ttttcacatc ccagtaattc aggcgatcgc ccagcgtact gccatcgcgc
     9001 ttttcccgcc ctttatcggc agcaagaaag aaattcatcc cttgtaacgg cccttcgctg
     9061 acccgatagt tcataccacc taagataccc gcgccttcaa tccgcacatc tcgtttactg
     9121 gattcatagc cgcgagtcac aatcggtgca aatgtcagcg taaattgcgg agttacacgg
     9181 tagttgacac ctaattgcca ggactgcatt ccttcacggt tacgatcaag acttaatgaa
     9241 aagggatagc caatgtcggt atccaccacg gcaccattca ttgcatggcg caaataacca
     9301 ccactgccaa atacgcttcc ccacggcgca ttctaggtca attgtccact aaccaacgcc
     9361 gtttcattgg gctggctggt attacgactt aaaccttcga gctgcgcgta aaagccgagc
     9421 aactcccctt tcaacgtata attcgggtcg agcggtatgc ttttctcgag aatactttgt
     9481 atctgattgg aaacatcttt ctgtaccgat gcccccagag aatagcgact ggtatcatca
     9541 tgccaactgg cggctaaaaa agaacgtgcg cgatgtttat cgtattttcc cgtctggctg
     9601 ttatacaccc cttcatcacg aataccggtt gttgaatcag tccataaaaa tactcgtgaa
     9661 cgccacattc cccacagttc aatatttggc gttaaagccc aataacctaa tgctccttca
     9721 gaaactgtcg gtaatgtacg ttgcgaactg ccagccatta acggaatacg ataaggtaag
     9781 ctaccataac tataatccgt cccgaagcga cctaatttaa gcaggctatt ttcactgggt
     9841 tttattttta ctgcccccag ggcaaagcca aaacttttat caccatcaag ataccaccgg
     9901 gtactcatat cagcacgagc acctaattta taaacataat acgctccgcc ttcaacgcca
     9961 ataatattgg aataataacc actattgaaa tcgagcaatc ctccctgtac ccatgcgtaa
    10021 atatctcttc ccgcgccagc actgggctta tcagccctgc gcaattcgtt tttcagtctg
    10081 atgcgtagtc ccgcttcatc gtcagccagt tgcgccgcgt aagcagaggt aaggccagag
    10141 gcagccgtca ggcaaataac ggccatggcc actatttttc tcatagttgc acctttgatg
    10201 ttctgattat ttttattgaa tattaattag tgatatcgct gattaattgc tgctgcactt
    10261 ttttacgatt atcaatttca accacgattt ctttgaattt tttatccgtg agcggataaa
    10321 accagataat aacgaatgcc agtagcataa atccgcaagg tactaaggca attgatgtgc
    10381 ggatgcccat aataacttcc ggcgtttaca cctgattggc gatatatccg cttaatccca
    10441 aaataaaggc aggaattgaa cctccgattg cctgaccgca tttacgggta aatgagaata
    10501 gtgaataggt gagcccttca attcgcacgc cggtcaggta ttcaccgtat tctacggtat
    10561 cagcttccag tgcccacatc acagtcatag taacgccctg accaagtgaa cagcccggta
    10621 tcatttaaca catagcgcac gtagaacaac gacgaggcgc tgaccgcgaa ggtcgaaatc
    10681 agcacacaca gcgcaccgat gcacaacata aacagcgggc gattccgttt cagggtttgc
    10741 aaactgatct tcaatgacgg ctgcgccacg atacgtacca cattttcacg cgtcgatttg
    10801 aagcagatga agtaaagcac cattccggca atcgccagca caatcgtcca gaaatggtat
    10861 accgacacca tctcttccgg gctggagttc ttaatgctcg gtcctatcag aaatgccagg
    10921 catacaaagg tcaatgaagc ggcaatccca cgagccgcgc ccagacgggc gcgggattgt
    10981 ggttgttggg tcatcgcggt agcaagtgaa ccataaggaa tattcaccag gctgtagcaa
    11041 agcccgaggc ccatgtaggt caaatatgca tacaccactt tgctaccatg gctccagtcg
    11101 gtcggtaccc agaataccag cacgctgaag atcattaacg gcgcagtacc gaagagtaaa
    11161 aacgggcgga attttcccca gcgggtattc acactgtcca ccactcgtcc ggcaaagacg
    11221 tcggcgaagg catcgaatac ccgcaccagt aacagcatgg tgcctgccgc agcggcaccg
    11281 acgccagcga cgtcggtgta gtaactcaat aggaagagcg cccccattgc gaaggcgaag
    11341 ttattggcga cgtcaccgag gctgtagccg acgatggtgc gccaggagag ttgttgattc
    11401 attgtttgcc tccctgctgc ggtttttcac cgaagttcat gccagtccag cgtttttgca
    11461 gcagaaaagc cgccgacttc ggtttgcggt cgcgagtgaa gatccctttc ttgttaccgc
    11521 caacgcgcaa tatgccttgc gaggtcgcaa aatcggcgaa attccatacc tgttcaccga
    11581 cgacggcgct gacgcgatca aagacgcggt gatacatatc cagccatgca cactgatact
    11641 cttcactcca catgtcggtg tacattgagt gcaggccggc taacgtatcc acgccgtatt
    11701 cggtgatgat aatcggctga tgcagtttct cctgccaggc cagcagttct ttttccagta
    11761 ccttctctgc cgtttccaaa tcgccgcttt ggacatacca accgtaataa cggttcaggc
    11821 acagcacatc aaagagatcg ctgatggtat cggtgtgagc gtcgcagaac attacattga
    11881 cgcaggtgat cggacgcgtc gggtcgagtt tacgcgttgc ttccgccagt ggcgcgaaat
    11941 attcccgtgc accttgcgga cgggtatccg gttcgttggc aatactccac atcaccacgc
    12001 ttgggtggtt tttgtcacgc gctatcagct ctttaatcgc ctgtaagtgc gcctgctgag
    12061 tttccccgtt gacagcctct tcgctgtaca gttctttcgg cttgttgccc gcttcgaaac
    12121 caatgtctaa agagaggtta aagccgacag ctgcagtttc atcaatcacc acgatgccat
    12181 gttcatctgc ccagtcgagc atctcttcag cgtaagggta atgcgaggta cggtaggagt
    12241 tggccccaat ccagtccatt aatgcgtgat cgtgcaccat cagcacgtta tcgaatcctt
    12301 tgcctcgcaa atccgcatct tcatgacgac caaagccagt aaagtagaac ggtttgtggt
    12361 taatcaggaa ctgttcgccc ttcactgcca ctgaccggat gccgacgcgc agcgggtaga
    12421 tatcacactc tgtctggctt ttggctgtga cgcacagttc atagagataa ccttcacccg
    12481 gttgccagag gtgcggattc accacttgca aagtcccgct ggtgccttgt ccagttgcaa
    12541 ccacctgttg atccgcatca cgcagttcaa cgctgatatc accattggcc accacctgcc
    12601 agtcaacaga cgcgtggtta cagtcttgcg cgacatgcgt caccacggtg atatcgtcca
    12661 cccaggtgtt cggcgtggtg tagagcatta cgctgcgatg gatcccggca tagttaaaga
    12721 aatcatggaa gtaagactgc tttttcttgc cgttttcgtc ggtaatcacc attcccggcg
    12781 ggatagtctg ccagttcagt tcgttgttca cacaaacggt gatacgtaca cttttcccgg
    12841 caataacata cggcgtgaca tcggcttcaa atggcgtata gccgccctga tgctccatca
    12901 cttcctgatt attgacccac actttgccgt aatgagtgac cgcatcgaaa cgcagcacga
    12961 tacgctggcc tgcccaacct ttcggtataa agacttcgcg ctgataccag acgttgccca
    13021 cataattacg aatatctgca tcggcgaact gatcgttaaa actgcctggc acagcaattg
    13081 cccggctttc ttgtaacgcg ctttcccacc aacgctgatc aattccacag ttttcgcgat
    13141 ccagactgaa tgcccacagg ccgtcgagtt ttttgatttc acgggttggg gtttctacag
    13201 gacgtaacat aagggactcc tcattaagat aaaaatactg gtcaaccttt aatctgatta
    13261 gttaaccaat tctgtgttgc acacagttat ctgtgagaaa cccatggaag agaaatggaa
    13321 ataaaaagaa ctgacaccgc gatcaaaaaa ccaaaaactg caatacccac agcgtgatat
    13381 ggatcgcatt aatctttaaa acagcgttga cagaagccat aaaataagca accgaacgtt
    13441 cgttcgagag tattgggaac gataaactct catgacatat ggctacggcg aatattttgg
    13501 gggggaattc tccgaacacg gggaggataa tttttgcaca gagaaattac gatatgaaac
    13561 gcgggcacag ttatcaccag cccgcgaaat gagaggatca ggatgcggtt aatataccgc
    13621 caatcatcgc gcgtaatccc tgagcaaacg acgcttcacg cgccaggtct tccgcttcga
    13681 tatcggccag tacaccgtag gtcagatcca gcagtaaacg tgaagtcatt gccgtgttga
    13741 tgtctgggct tatctcgcct ttttgctggg catcacgcat ccgctgggca acaaattcag
    13801 tgatcgtcat atgcttattt ttaagcatgg cggcgacctg tgggttacgc atcccttccg
    13861 ccataatttc gaccaccagc gcccgttgcc caaaggcttc atgggtgaga gagacaatag
    13921 actcgaccat atagtcaaca aaatgaatcc cttcaatcgg ttcccggaaa cgagccagcg
    13981 ccctctcctg gtcctgtaag ataatcgcct gaatcaaggc ttctttggag atgaaatggt
    14041 gatagagcgt cccaggacta atggcgcaag atttacagat ggctttcatc gaggcactgt
    14101 gaaatccatt ttctgaaaaa atctctctgg cagcattgag gatccgggtc cgtgtcggtt
    14161 gtgcttcagt ctgcatgtta tccatcatta aaccattata gttagcgtga ataactagtt
    14221 taccgcgttg tcaggtgagc gcctatcatt gctgttgaat agtgcgaagg tacactctat
    14281 tcgtatactt ttcacccagc aattggttac ccaatagccg tgatttggct gccagttgtt
    14341 tagcagagac gttgatgaat tgcacacgct atcccggtaa acaacacata cgaccgttta
    14401 ccggttcgtt agtgtattaa ttgagctcct gtaccccacc accggagacg gtgagaattt
    14461 gtccgcttac ccagctggct gcaggcgagc aaaggaacag cgccgcgtta gcaatatctt
    14521 gcggttggcc cagacgtctg atcggcgtgt gctgtaacat tttttgttca atttctggtg
    14581 taataacgga tttcagggca tcggttaata ttgcccccgg cgcaatgcca ttcacccgaa
    14641 tatttttttc acccaggtca aatgccatat ttctgaccag atgactggcc gcagctttag
    14701 atgatgcata ggaagtcatg tttatatttt tattttctgc cgccatagaa gtgatggtca
    14761 gaataacgcc accgccattt ttttccattt ctggcgcaac aagttgtgac agatggaaaa
    14821 aagaaaacac attcagttca taagcacggc gaaaatctgc cattggcata tcaaacggtt
    14881 taggaccacc gccaccggcg ttgttaacca ggatatcaac tttacccagt ttactgacgg
    14941 caaagtctgc cagtgcagag agttcctgtt cggaagtaat atcacaacgc caggcaaatg
    15001 cctgaccacc cagttgttga atttcgtcta caacatggtt agctgcgtcg gcgttaatat
    15061 cactgaccac cacagatgcg ccagctgtcg cgaatgtaat ggcgatttct ttaccaatac
    15121 ctgcacccgc acctgtgatg atggcgcatt ttccgtcgag tctcaggttg tcagaattaa
    15181 acacataaac ctcctgtgaa cttcagtaaa aataaaaggc accgcctg
//
LOCUS       DADLSE010000093        15049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLSE010000093 DADLSE010000000
VERSION     DADLSE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.093"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..238
                     /locus_tag="JN692_002805"
     CDS             <1..238
                     /locus_tag="JN692_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7265078.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="JN692_002806"
     CDS             248..886
                     /locus_tag="JN692_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7265079.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNSYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="JN692_002807"
     CDS             889..2052
                     /locus_tag="JN692_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7265080.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..3761
                     /locus_tag="JN692_002808"
     CDS             2136..3761
                     /locus_tag="JN692_002808"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HAY7265081.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASSPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN692_002809"
     CDS             complement(3878..4153)
                     /gene="yjfN"
                     /locus_tag="JN692_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7265082.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN692_002810"
     CDS             complement(4302..4631)
                     /gene="bsmA"
                     /locus_tag="JN692_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7265083.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4813..5562
                     /gene="yjfP"
                     /locus_tag="JN692_002811"
     CDS             4813..5562
                     /gene="yjfP"
                     /locus_tag="JN692_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7265084.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGALMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN692_002812"
     CDS             complement(5559..6314)
                     /gene="ulaR"
                     /locus_tag="JN692_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7265085.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN692_002813"
     CDS             complement(6422..7486)
                     /gene="ulaG"
                     /locus_tag="JN692_002813"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7265086.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGKALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7841..9238
                     /gene="ulaA"
                     /locus_tag="JN692_002814"
     CDS             7841..9238
                     /gene="ulaA"
                     /locus_tag="JN692_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7265087.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIISFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDNYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9254..9559
                     /gene="ulaB"
                     /locus_tag="JN692_002815"
     CDS             9254..9559
                     /gene="ulaB"
                     /locus_tag="JN692_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7265088.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9569..10033
                     /gene="ulaC"
                     /locus_tag="JN692_002816"
     CDS             9569..10033
                     /gene="ulaC"
                     /locus_tag="JN692_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HAY7265089.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10047..10697
                     /gene="ulaD"
                     /locus_tag="JN692_002817"
     CDS             10047..10697
                     /gene="ulaD"
                     /locus_tag="JN692_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7265090.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10707..11561
                     /locus_tag="JN692_002818"
     CDS             10707..11561
                     /locus_tag="JN692_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7265091.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ECLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11561..12247
                     /locus_tag="JN692_002819"
     CDS             11561..12247
                     /locus_tag="JN692_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7265092.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12376..12651)
                     /locus_tag="JN692_002820"
     CDS             complement(12376..12651)
                     /locus_tag="JN692_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7265093.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12978..13373
                     /gene="rpsF"
                     /locus_tag="JN692_002821"
     CDS             12978..13373
                     /gene="rpsF"
                     /locus_tag="JN692_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7265094.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTVAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13380..13694
                     /gene="priB"
                     /locus_tag="JN692_002822"
     CDS             13380..13694
                     /gene="priB"
                     /locus_tag="JN692_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7265095.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13699..13926
                     /gene="rpsR"
                     /locus_tag="JN692_002823"
     CDS             13699..13926
                     /gene="rpsR"
                     /locus_tag="JN692_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7265096.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13968..14417
                     /gene="rplI"
                     /locus_tag="JN692_002824"
     CDS             13968..14417
                     /gene="rplI"
                     /locus_tag="JN692_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7265097.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEVRRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14488..15045)
                     /locus_tag="JN692_002825"
     CDS             complement(14488..15045)
                     /locus_tag="JN692_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7265098.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3512 a         3659 c         4066 g         3812 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatagct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacgttttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaacc gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcacgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccaata acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggttccggca
     3421 atattatgtg cctggatgtg ttgcgcgttc tcaataagca agcgggcgta tacgacttat
     3481 tgtcggaagc atttgtggaa gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc
     3541 gtttacagca gcagctgcgt aagccagctg aagaactggg gcgagagatt actcatcagc
     3601 tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ttcttcgcca atggcgcagg
     3661 cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag cagatccaga
     3721 atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg actgatgcga
     3781 cgctggtttc gattaactaa atgaaatatg tgaaaattgt aggccggaca aggcgctcgc
     3841 gccgcatccg gcattgttca tttgctgtag atgacactca tgcatacagt atcgcctgta
     3901 cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac catgacgcac
     3961 cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc cctgaaatat
     4021 tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca ctggcgaatt
     4081 ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa
     4141 gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg gcattccatc
     4201 gcggtaataa acgcccaagc ttccagccgg taatttattg tgcacaggta aacgcatagg
     4261 gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg gtacaaaatg
     4321 gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa
     4381 tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc cggagaaccc
     4441 cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc
     4501 tcttgcggat ggtccgttac tggcggtgct ggctgtggcg taccttgcag tgcgctacag
     4561 gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct attacgtttc
     4621 ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc ccgaattgtt
     4681 acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat ctcaacatta
     4741 tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat accagacaaa
     4801 aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc cgttcttcat
     4861 gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta tcacggcttt
     4921 acttcatcca gcctggtgta tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg
     4981 gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggcgacga agcgcggcgg
     5041 ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac tttacgtgcg
     5101 gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg cgcgttgatg
     5161 ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg taccgccagc
     5221 atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc gctgatacct
     5281 gaaacggcag cacagcagaa tgaattcaat aaaattgtcg cgccgctggc agagtgggaa
     5341 gcgacaaacc acctggaaca acttggggac agacctctac tgctgtggca tggcctcgac
     5401 gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag cgagacggga
     5461 cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat tacgcctgag
     5521 gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag aatgctgacc
     5581 ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt gatcagcata
     5641 tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt actgctatca
     5701 accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat tgctgtcagc
     5761 atatcggttt tatacaaccc ttctgcggtc agcccttttc cgctggtaaa catccagtgc
     5821 ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg acttttgttg
     5881 tactgtccac ccataatgat cacgctgtca tgttcttgat cgatcaggta atttgccagc
     5941 ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc aagcagaaac
     6001 gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg cgaggccgct
     6061 ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat cggcgtccag
     6121 cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag tttgccgctt
     6181 tcgccaagtt tattgatatc gcgtcgcgca gtggcaggcg aaattcccag acgctcaacg
     6241 actttctcaa cggtcacaaa gcccaattgt gcgagcattt ctaggaggat ttgatgtctt
     6301 tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat atttgaaata
     6361 gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta taaacgacag
     6421 attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca tcgaaaccgc
     6481 gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg ccgccaacct
     6541 gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc cacagcacgc
     6601 ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac gggataacca
     6661 ctttcgcatt cagggcttta cccatacgca gcatatcggc gctggtcatt ttgtcggtaa
     6721 taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt tcgttaccgt
     6781 gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa ccgccagggg
     6841 ttttgaacag gtagtttact gcgcgatcgt ccatgccatc tggcagtacg ccagccgctt
     6901 tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc gcatgaattt
     6961 caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc tctttcggta
     7021 cgccccagcc aatccacaaa tcaacgcagg ttttcggtcc aatgaatggt acgtcatcag
     7081 cacagttctg catcacggca gcagcgacgt taacgtcgat atgatcgttg tgatcgtgag
     7141 tcgccagtac cgcgtcgatc tggcgaatcg caaacggatc aagaacaaac ggggtggtgc
     7201 gtaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catctggtga ccctgtttca
     7261 ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg caaacgttgg
     7321 tgccaccttc tgatttcaac cagatcccgg tgcagcctag ccaccacatc gcaaatgtgc
     7381 caggggcgac ctgctcttgt tcaatttctt cattcagcca gctaccccac tccgggaaag
     7441 tgctcaggat ccaggattct cgggtgatac ttttcacttt actcatcgcc atttaccttc
     7501 atgatagttc aattcgaatc aatatgtgat tggttttgat taatcctgac actatttttt
     7561 caggaaggca atgaccattt tttgactttt tgccagggaa gttgttgttg attttgagtg
     7621 tggaaagatt taatggaatg tgtagttaaa ttaactaaat gaatttaaat aaataattgt
     7681 ttcgttgtgt gaatcccgct ctatccgtgt ggaattattt gcgggtcgcg tcacatttaa
     7741 tcataaataa tcttgttgtg attacttttg aaaattagag tgagtgcaca acattccggg
     7801 tgtgcggaat acccggttac ctcttcttca ggagatcgtt atggagatcc tctacaacat
     7861 ctttaccgtg ttttttaacc aggtcatgac caatgccccg ttgttgctgg gtattgtgac
     7921 ctgtctgggc tacatcctac tgcgcaaaag tgtcagcgtt attattaaag gcacgattaa
     7981 aaccataatt agtttcatgt tgttgcaggc agggtccggc atcctcacca gcaccttcaa
     8041 accggtggtg gcaaaaatgt ccgaagtcta cggcattaac ggcgcaattt ccgataatta
     8101 cgcgtcgatg atggcaacca tcgaccgcat gggcgatgcc tatagctggg taggttacgc
     8161 ggtgttgtta gcgctggcgc tgaacatctg ttacgtgctg ctgcgtcgca ttaccggtat
     8221 tcgtaccatc atgctgactg gtcatatcat gttccagcag gcggggctga ttgccgttac
     8281 gttgtttatt ttcggctact ccatgtggac aaccattatc tgcacggcga ttctggtttc
     8341 gctctactgg ggcattacct ccaacatgat gtacaagccg actcaggaag tgacggacgg
     8401 ctgcggtttc tccatcggtc accagcaaca gtttgcatca tggattgcct ataaagttgc
     8461 gccgttcctc ggcaaaaaag aggaaagcgt tgaagacctc aaactgccag gctggctgaa
     8521 tattttccac gacaacatcg tctccacggc gattgtcatg accatcttct ttggtgccat
     8581 tctgctctcc ttcggtatcg acaccgtgca ggcgatggca ggcaaagtgc actggacggt
     8641 ctatatcctg caaaccggtt tttccttcgc ggtggcgatc ttcatcatca ctcagggtgt
     8701 gcgcatgttt gtggcggaac tctctgaagc gtttaacggt atctcccagc gcctgatccc
     8761 tggtgcggtt ttggcgattg actgtgcggc tatttatagc ttcgcgccga acgccgtggt
     8821 ctggggcttt atgtggggca ccatcggtca gctgattgcg gttggcatcc tggtcgcctg
     8881 cggttcctcg atcctgatta tcccaggctt tatcccgatg ttcttctcca acgccaccat
     8941 cggcgtgttc gctaaccact tcggcggctg gcgtgcggcg ctgaagattt gtctggtgat
     9001 ggggatgatc gaaatctttg gttgcgtctg ggcggtgaaa cttaccggta tgagtgcctg
     9061 gatggggatg gcggactggt cgattctggc accgccgatg atgcagggct tcttctccat
     9121 cggtatcgcc tttatggccg tcatcattgt aattgcactg gcttatatgt tcttcgctgg
     9181 ccgcgcactg cgcgcagaag aagatgcaga aaaacaactg gcagaacagt ctgcttaata
     9241 aggagttttg attatgaccg tacgtattct ggctgtgtgt ggcaacggac aaggcagttc
     9301 catgatcatg aagatgaaag tggaccagtt tttaacccaa tcaaacattg accacacggt
     9361 aaatagctgc gcggttggcg aatacaaaag cgagttgagc ggcgcggata tcatcatcgc
     9421 ttctacgcac attgcgggcg aaatcaccgt gaccggcaac aaatacgtgg ttggcgtgcg
     9481 caacatgctc tctcctgccg actttggccc gaaactgctg gaagtgatca aagcgcattt
     9541 cccgcaggat gtgaagtaag gacgcgccat gaaattacat gattcgctgg cggagaataa
     9601 atccatccgc ctgcaggctg aagctgagac atggcaggac gcagtaaaaa tcggcgttga
     9661 cctgctggtg gcggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt
     9721 tgaacagcac ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc
     9781 ggaagagggc gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt
     9841 caaccacgaa gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa
     9901 cacgcaccag gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt
     9961 cgaccgttta cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa
    10021 cgcggcagct taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca
    10081 accagactat ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta
    10141 tcgaagtggg taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag
    10201 cgctctaccc gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc
    10261 tttctcgtat gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata
    10321 tcaacaccgc caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg
    10381 aactgaccgg ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc
    10441 aggtggttta tcaccgcagc cgtgacgcgc aggccgctgg cgtggcgtgg ggtgaagccg
    10501 atatcactgc gatcaaacgt ctttccgata tgggcttcaa agtcaccgtc accggtggcc
    10561 tggcgctgga agatctgccg ctgttcaaag gcattccaat tcatgtgttt atcgccggtc
    10621 gcagcatccg tgatgccgct tctccggttg aagccgcgcg tcagttcaaa cgttccatcg
    10681 ccgaactgtg gggctaagga gcggatatgt tgtccaaaca aatcccgctt ggcatctatg
    10741 aaaaagcgct ccccgccggg gagtgctggc tggaacgcct gcgactggca aaaacgttgg
    10801 gcttcgattt tgtcgagatg tcggtagatg aaactgacga gtgcctgtcg cgcctcgact
    10861 ggagccgcga gcagcgtctg gcgctggtga atgcgattgt tgaaaccggc gtgcgtgtgc
    10921 cgtccatgtg cctttctgcc catcgtcgtt tcccgttggg cagcgaagat gacgcggtgc
    10981 gggcgcaggg gctggagatt atgcgtaaag ccatccagtt cgcccaggac gttggcattc
    11041 gcgtgatcca gctggcgggc tatgacgttt actatcagga agccaataac gaaacgcgtc
    11101 gtcgtttccg tgacggcctg aaagagagcg ttgagatggc gagccgcgcg caggtgacgc
    11161 tggcgatgga gatcatggat tatccgttga tgaactccat cagcaaggcg ctgggttacg
    11221 cgcactatct caacaacccg tggttccagc tctacccgga tatcggcaac ctgtcggcgt
    11281 gggacaacga tgtgcagatg gagttgcagg ccggaatcgg gcatatcgtc gcggtgcatg
    11341 tgaaagacac caaaccgggc gtcttcaaaa acgtgccatt tggcgaaggt gtggtggatt
    11401 tcgaacgttg cttcgaaacg ctcaaacaga gtggctattg cgggccgtac ctgattgaga
    11461 tgtggagcga aacggcggaa gacccggcgg cagaagtggt gaaagcgcgt gattgggtga
    11521 aagcgcgcat ggccaaagca ggcatggtgg aggcggcata atgcaaaagc taaaacagca
    11581 ggtgtttgaa gccaacatgg atctgccgcg ctatgggctg gtgaccttta cctggggcaa
    11641 cgtcagcgct atcgaccgcg aacgcgggct ggtggtgatc aagcccagcg gcgttgccta
    11701 cgaaaccatg aaagcagacg atatggtggt ggtcgatatg agcggcaagg tggtggaagg
    11761 ggagtatcgc ccatcttccg acactgcgac gcatctcgaa ctctaccgtc gttacccgtc
    11821 gcttggcggc attgtccata cccactccac tcatgccacc gcatgggcgc aggcggggct
    11881 ggcgatcccg gcgttaggca ccacgcacgc tgactacttc tttggtgata taccgtgtac
    11941 gcgtggatta agcgaagaag aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat
    12001 tatcgaaacg ctgggcaacg ccgagccgct gcatacgccg ggaattgtgg tgtatcagca
    12061 cggaccgttc gcctggggga aagatgccca cgacgcggtg cataacgcgg tagtgatgga
    12121 agaagtggcg aaaatggcgt ggattgcgcg cagtattaat ccacaactca atcatatcga
    12181 cagctacctg atgaataaac acttcatgcg taagcacggt cctaacgctt attacgggca
    12241 gaagtaaaac acgcgctgcg gaaatttcct tcctcgggag ataactggtc taattccgca
    12301 gccgtttttc aaaaaaagcc ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt
    12361 tggttatacc tggtgctagc gataaatatc cgcgctggcg tgcatattgc cgttactccc
    12421 cggttcccgc atcagaatta cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt
    12481 taatgcctga cgtgcttcgc tttcagtggc gaaattatga ttgatataaa tcacgcctaa
    12541 gctttgcaca tcgtccatat ttctggcctg gtggttatta atttcaatgg ctgcccatgt
    12601 atttgcactt agcaaaagca cagccagaag ggctaaaact cgactgaaca tagatacctc
    12661 ctcgacggct gactttgtgt gctctcctct gtgatgatct tctgatttaa ttttaatcaa
    12721 tgataaagaa gttgatggtg accatttctg atgcagttgt tcaaaaaaac actgtgacgg
    12781 aatgtgatga acttcaaatc agcggtttag aggttatttg cgaaagggga gatttatttc
    12841 tgctccgccc ttgagtttag cggggtatgt cagtactata acgcgtcatt tttcagccga
    12901 cctttaacac gttccttgcc tccccgggat tcggctgacc cagacaggag gctgaataat
    12961 ccgtaaggag caattcgatg cgtcattacg aaatcgtttt tatggtccat cctgaccaga
    13021 gcgaacaggt tccgggcatg atcgagcgct acactgttgc catcactggt gcagaaggca
    13081 agatccaccg tctggaagac tggggccgcc gtcagctggc ttacccgatc aacaaactgc
    13141 acaaagcaca ctacgttctg atgaacgttg aagctccgca ggaagtgatc gatgagctgg
    13201 aaactacctt ccgcttcaac gatgccgtta tccgcagcat ggttatgcgt accaagcacg
    13261 ctgttaccga agcatctccg atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg
    13321 atttcgcaaa cgaaaccgct gatgatgctg aagctgggga ttctgaagag taatttctga
    13381 tgaccaaccg tctggtgttg tccggcaccg tgtgcaggac tccccttcga aaggtcagtc
    13441 catcaggaat tcctcactgc cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg
    13501 gctttcaccg gcaggcatgg tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg
    13561 ccattactca cagtataacg gtcggcagtc gcataaccgt tcaggggttc atttcatgcc
    13621 acaaggcaaa gaacggactg agcaaaatgg ttttgcatgc cgagcagatt gaattgatag
    13681 attctggaga ctagccatat ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc
    13741 gcggaaggcg ttcaagagat cgactataaa gatatcgcta cgctgaaaaa ctacatcacc
    13801 gaaagcggta agattgtccc aagccgtatc accggtaccc gtgcaaaata ccagcgtcag
    13861 ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc tgccgtacac tgatcgccat
    13921 cagtaatcgg tcacggtcca ttaatacgac tttgagagga taaggtaatg caagttattc
    13981 tgcttgataa agtagcaaac ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct
    14041 atgctcgtaa cttcctggta ccgcagggta aagctgttcc agctaccaag aaaaacattg
    14101 aattcttcga agtacgtcgc gctgaactgg aagctaaact ggctgaagtt ctggcagctg
    14161 ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt taccatcgcg tctaaagctg
    14221 gcgacgaagg taaactgttc ggttccatcg gtactcgcga catcgctgac gctgtaactg
    14281 cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca
    14341 ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa
    14401 acgtagtagc tgaataattc gttattcaac gagacgtaaa aacgccgacc attggtcggc
    14461 gttttgcttt ctatttttcg tcaggcatta atttcgcaag tagatccatt caccattctc
    14521 ttgtgtccat aagtcaggat ggtttgctgg cgttagatgc atacgatcac tccttatgtc
    14581 tcggtcctgc ggcactcgct cgaaacctaa acccgagtag attgcggctg aatttgataa
    14641 ggaacgtaga ccaattttca aatcatgggg taaatcagaa tacgcgttta gcaatgcagc
    14701 aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg cgaagttggt cgatataaag
    14761 atcgattgtt ggtagttcag atgaccagca attaggcatt tgggcaggaa gggttacaac
    14821 gtatcctatc gtttggccgg tatagtctgg agtactataa ccaggtccca aaacaacctc
    14881 gtgtattgtg tatttactgt tgggttcgaa ggattcgaca tcttgcaata aatatttcag
    14941 ttctgcggtt aacctcgcac ccgcgtaagc tttattgtat ttctgggcgt gaatattatc
    15001 taatgtagct tcatggttcc atccaaaaga attatcacca ttcattgtt
//
LOCUS       DADLSE010000094        14637 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLSE010000094 DADLSE010000000
VERSION     DADLSE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.094"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN692_002826"
     CDS             complement(86..2062)
                     /gene="speA"
                     /locus_tag="JN692_002826"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7265099.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN692_002827"
     CDS             complement(2071..2202)
                     /gene="yqgB"
                     /locus_tag="JN692_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7265100.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2338..2553
                     /locus_tag="JN692_002828"
     CDS             2338..2553
                     /locus_tag="JN692_002828"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265101.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2857..4011
                     /gene="metK"
                     /locus_tag="JN692_002829"
     CDS             2857..4011
                     /gene="metK"
                     /locus_tag="JN692_002829"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7265102.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4448..5842
                     /locus_tag="JN692_002830"
     CDS             4448..5842
                     /locus_tag="JN692_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7265103.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5919..6416
                     /locus_tag="JN692_002831"
     CDS             5919..6416
                     /locus_tag="JN692_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7265104.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6511..7218
                     /gene="endA"
                     /locus_tag="JN692_002832"
     CDS             6511..7218
                     /gene="endA"
                     /locus_tag="JN692_002832"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7265105.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7298..8029
                     /gene="rsmE"
                     /locus_tag="JN692_002833"
     CDS             7298..8029
                     /gene="rsmE"
                     /locus_tag="JN692_002833"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7265106.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8042..8992
                     /gene="gshB"
                     /locus_tag="JN692_002834"
     CDS             8042..8992
                     /gene="gshB"
                     /locus_tag="JN692_002834"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7265107.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9029..9664
                     /locus_tag="JN692_002835"
     CDS             9029..9664
                     /locus_tag="JN692_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7265108.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKISPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9664..10080
                     /gene="ruvX"
                     /locus_tag="JN692_002836"
     CDS             9664..10080
                     /gene="ruvX"
                     /locus_tag="JN692_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7265109.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10319..11236)
                     /locus_tag="JN692_002837"
     CDS             complement(10319..11236)
                     /locus_tag="JN692_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7265110.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTG"
     gene            11254..11958
                     /gene="yggS"
                     /locus_tag="JN692_002838"
     CDS             11254..11958
                     /gene="yggS"
                     /locus_tag="JN692_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7265111.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11976..12542
                     /gene="yggT"
                     /locus_tag="JN692_002839"
     CDS             11976..12542
                     /gene="yggT"
                     /locus_tag="JN692_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7265112.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQSTGNMLLPGLWMAL"
     gene            12539..12829
                     /locus_tag="JN692_002840"
     CDS             12539..12829
                     /locus_tag="JN692_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7265113.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12837..13430
                     /locus_tag="JN692_002841"
     CDS             12837..13430
                     /locus_tag="JN692_002841"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7265114.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13423..14559
                     /gene="hemW"
                     /locus_tag="JN692_002842"
     CDS             13423..14559
                     /gene="hemW"
                     /locus_tag="JN692_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7265115.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         3531 a         3871 c         3753 g         3482 t
ORIGIN      
        1 gcagctagat tatttttaag aatatataaa aagaaagcgg gccgatttac gtaaataaat
       61 cgacccgcca gcggaataat cttaattatt catcttcaag ataagtataa ccgtacaaac
      121 ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg gttttcttca
      181 cttgatcgcg gaactgggtt aacagcgttt tcggatcgag ctgtacatat tgcagcatgt
      241 ccgccacggt atcgccttcg tcagacagtt ctacttctac gctaccgtca gggaacacga
      301 acacgtcaac tgcttcggta tcaccgaaca ggttgtgcat attgccaagg atctcctgat
      361 atgcaccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac tccggcattg
      421 gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg tcagagtcac
      481 aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct tccagcggta
      541 gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac agcgagaaat
      601 tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga cgatgcgcac
      661 ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag agttgttctg
      721 cccatgcacg ttcttgcaag ctaaaggtgc cggaagagta gccgatatga atgtcgtgca
      781 gatccatctg gctgtcgtgt aaccattcac gcaaagaacg gcgagttccc ggttcgtgca
      841 tttcctgcca ggtttcccac atgctttgca gcgcacgcgg cgcatcttct gcgggcgcgg
      901 tcggcaccgt atattcgtta cgttccacgc cgatgatatt cgacaccagc acggtatggt
      961 gcgcagtcac cgcacggccc gattcggtga ttaccgtcgg atgcggcaga ccattttctt
     1021 cacacgcatc gccaatcgcc cagatgatgt tgttggcgta ttcattgagg ccatagttta
     1081 cggagcagtc agactgcgaa cgggtgcctt cataatccac gcccagaccg ccgccgacgt
     1141 cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc gcggattcac
     1201 gaacgcctgt cgcgatatcg cgaatattcg ccatctgcga accgaggtgg aagtgcagta
     1261 gttgcaggct gtcgagacgc ccggcttcac gcagggtttc aaccagttgc agtacctgag
     1321 tcgcagccag gccgaacttc gatttttccc cgccggagga ctgccattta cccgagccct
     1381 gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt tctgcttcat
     1441 ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg tgccccatct
     1501 tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg cagacgatga
     1561 cgctacgagt catgccagca tgtgccagca ctgccatcaa ctcggctttg gaaccggctt
     1621 ccagacccag cggttcaccc gaatgaatca gggactcgat cacgcggcgg tgctggttaa
     1681 ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc ctcgcacgtt
     1741 tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa cagaacagtg
     1801 caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga tcgacgcgag
     1861 cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta acgtcatagt
     1921 agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct tcctgggagc
     1981 tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac gaaggcaaac
     2041 ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg taaaacagtt
     2101 aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat tttccagcag
     2161 tgtatgctga cgctccgact gcgcaaccgg tttctttttc ataacattat taagcacata
     2221 accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt ttacaagagc
     2281 gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag cgccgagatg
     2341 ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg aatcttcacc
     2401 agaacggtga gacaggttaa gcagcagaca acggttcatt atttcgtatc acctccacgg
     2461 ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct ggaagtggca
     2521 acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt ataccccaca
     2581 atggcagaaa attgcaaaag ataaatacgc agaatgccgg cattgtcagg aaaaatttcc
     2641 agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg ggtgaagaat
     2701 tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact cagactgcca
     2761 gtgtttttaa cctgcagagt cgtggtagga tccgctacca cagaaaatcc acacaacagt
     2821 ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct ttttacgtcc
     2881 gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga tgccgtttta
     2941 gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta cgtaaaaacc
     3001 ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat cgaagagatc
     3061 acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt tgacgctaac
     3121 tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca gggcgttgac
     3181 cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg ctacgcaact
     3241 aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct ggtacagcgt
     3301 caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc gaaaagccag
     3361 gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt gctttccact
     3421 cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga agagatcatc
     3481 aagccaattc tgcccgctga atggctgact tctgccacca aattcttcat caacccgacc
     3541 ggtcgtttcg ttatcggtgg cccaatgggt gactgcggtc tgactggtcg taaaattatc
     3601 gttgatacct acggcggcat ggcgcgtcac ggtggcggtg cattctctgg taaagatcca
     3661 tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa catcgttgct
     3721 gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt ggctgaaccg
     3781 acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca actgactctg
     3841 ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct ggatctgctg
     3901 cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca tttcccgtgg
     3961 gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta atctttcttc
     4021 acctgcgttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc gcggtggata
     4081 ggcgttcacg ccgcatccgg caaaaaaact gcccgcacaa taacatcatt cttcctgatc
     4141 acgtttcacg gcaaattacc atcacaactg aaaccgatta caccaaccac accagcaaaa
     4201 tatttgtaat attttcatat tgttattcag ctttcacagt tgttacattt cttttcagta
     4261 aagtcttaat tgcagatgtc agcgtttaat ctatgatgat ataactcaat tattttcatg
     4321 cacttaaatc ataactaaga taaatgttag tgtaagcgat tacactaatg tgatttgctt
     4381 cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa ccatattgga
     4441 gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga cgtttttcgt
     4501 ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg taattgctgg
     4561 cgcactgccg tttattgcag atgaattcca gataacttcg cacacgcaag aatgggtcgt
     4621 aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc tctcctttaa
     4681 actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg gttcactgtt
     4741 ctctgcggct gcgccaaacg ttgaagtact gattctttcg cgcgttctgc tggggctggc
     4801 ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc cggaaaaaat
     4861 tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc tcggtgctta
     4921 tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg gtgtgattat
     4981 catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc cacgttggtt
     5041 tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc gtgacaccag
     5101 cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta aacagagtgg
     5161 ctgggcgctg tttaaagaga atagcaactt ccgccgtgcg gtgttccttg gcgtactgtt
     5221 acaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc cgaaaatctt
     5281 cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga ttgtcggcct
     5341 gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg gacgtaaacc
     5401 aacgctaacg ctgggcttcc tggtgatggc tgctggcatg ggcgtactcg gtacaatgat
     5461 gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc tgctgatgtt
     5521 tattgtcggt tttgccatga gtgccggtcc gctgatttgg gtactgtgct ccgaaattca
     5581 gccgctgaaa ggccgcgatt ttggcatcac ctgctccacc gccaccaact ggattgccaa
     5641 catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg ccaacacctt
     5701 ctgggtgtat gcggctctga acgtactgtt tatcctgctg acattatggc tggtaccgga
     5761 aaccaaacac gtttcgctgg aacatattga acgtaatctg atgaaaggtc gtaaactgcg
     5821 cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag gaagccgcct
     5881 cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc cgtctcccta
     5941 tcgccatcca acaggccgtt atgcgccgcc tgcgggaaaa actcgcccag gccaacctga
     6001 agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga acctccgccg
     6061 gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc cgttttgctg ttggaaaaca
     6121 gtgaagcttt tattgaagaa gtggtaccgc acgaactggc acatttgctg gtatggaaac
     6181 atttcggccg cgtagcgcca catggcaaag agtggaagtg gatgatggaa agcgtgctgg
     6241 gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgtcgc aacaccttcc
     6301 cctaccgctg taagtgccag gaacatcagc ttaccgtacg ccgccataat cgcgtagttc
     6361 gtggcgaggc catctatcgc tgtgttcact gcggtgaaca gctggttgcg aaataaccac
     6421 ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac tttcgctacg
     6481 ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat tgctgcggtg
     6541 gtactgagcg cagcattttc cggcccgacg ttggccgaag gtatcaatag tttttctcag
     6601 gcgaaagccg cggcggtaaa agtccacgct gatgcgcccg gtacgtttta ttgcggatgt
     6661 aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg ctatcaggtg
     6721 cgcaaaaatg aaaaccgcgc cagccgcgta gagtgggaac acgtcgttcc cgcctggcaa
     6781 ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaactgcgc taaagatccg
     6841 gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg cgaggtgaat
     6901 ggcgatcgcg gcaattttat gtacagccag tggaatggcg gcgaaggcca gtacggtcaa
     6961 tgcgccatga aggtcgattt caaagaaaaa gctgccgaac caccagcgcg tgcacgcggt
     7021 gccattgcgc gcacctactt ctatatgcgc gaccaataca acctgacaca ctctcgccag
     7081 caaacgcagc tgttcaacgc atggaacaag atgtatccgg ttaccgactg ggagtgcgag
     7141 cgcgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca acgcgcttgc
     7201 caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc taccccacgc
     7261 gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc gcatttatca
     7321 tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg ccaaccatat
     7381 cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa ttgtttgacg gtagcaacca
     7441 ggtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga aggtgctgga
     7501 aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg tgatgtcgcg
     7561 tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa gcctcattac
     7621 gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga acaagaagct
     7681 tcagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggtcgta accgggtgcc
     7741 ggaaatccgt ccagcgatgg atctggaagc ttggtgtgca gagcaggatg aaggactgaa
     7801 actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgcctttac cggttgaacg
     7861 cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg ccatgactgc
     7921 ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtacag agacaactgc
     7981 gctcaccgcc attaccgcgc tacaagtacg atttggcgat ttgggctaac ggagaagaat
     8041 aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca agaaagattc
     8101 cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact atatggagat
     8161 gggcgatctg tatctgatca atggcgaagc ccgcgcccat acccgcacgc tgaacgtgaa
     8221 gcagaactac gaagagtggt tttcgttcgt cggtgaacag gatctgccgc tggccgatct
     8281 cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct acgcgaccta
     8341 tattctggaa cgtgccgaag agaaagggac gctgatcgtt aacaagccgc agagcctgcg
     8401 cgactgtaac gagaaactgt ttaccgcctg gttctctgac ttaacgccag aaacgctggt
     8461 tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa cacagcgaca tcattcttaa
     8521 gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg atccaaacct
     8581 cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg cgcaaaatta
     8641 cctgccagcc attaaagatg gcgacaaacg cgtgctggtg gtggatggcg agccggtacc
     8701 gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg ctgccggtgg
     8761 tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc agatcgggcc
     8821 gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg accgtctgac
     8881 tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt ttccggtgtc
     8941 gatcaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt aacccacctt
     9001 agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac gcatactggg
     9061 cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac agcatcactt
     9121 tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg tctacatttg
     9181 cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg aaaatctcaa
     9241 aattgaaggg attctggaaa agctgaagat ctcgccggag ccgcgtgatg aatcaatccg
     9301 tctggataaa ccggttatgc tcggcggccc actggctgaa gatcgcgggt ttattttgca
     9361 tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacgg tgatgaccac
     9421 ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg tattggtggc
     9481 tctgggctat gcctcctggg aaaaaggtca actggaacaa gaaattctcg ataacgcgtg
     9541 gctaacggcc ccggcggatc tgaatattct gttcaaaacg ccgattgccg accgctggcg
     9601 cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg caggacacgc
     9661 ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg cgtagcggtc
     9721 ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca ggacggtacg
     9781 ccggactgga acctcatcga acgtttactg aaagagtggc agcctgacga aatcatcgtc
     9841 ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc gcgcaaattt
     9901 gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga gcgtcttagc
     9961 actgtggaag cccgttccgg tctttttgaa cagggcggct atcgagcgct caacaaaggc
    10021 aaagttgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca gggatattaa
    10081 ggcgatctaa aagcctggcg gatagtcgtt accgccatct tgccggatgc ggcgtgaacg
    10141 ccttatccag cacacatctg gcagcggctg taggtctgat aagacgcgtt agcgtcgcat
    10201 cagacattta ttgcctttat aggccctata aacttgcgca tcgggcatgg caacgtcaca
    10261 aacgcccttc ccccacccgc tgctgataac tctgctgaaa cgttaacatc cccacctgct
    10321 acccggtttg aataacatgc ggtaactggt gggttttccc ttcgcgaatc aaattcccca
    10381 ccgcgggagt gttaatcagt aattcaaaca gcgccacgcg tccttcctgt ttatccactt
    10441 ccagcttttg tgacagcact gcccgtaaac tacccgcaag ttgattacgt acgggatctt
    10501 tttcctgcgc cggaaatgaa tccaccagtc gctcaactgc ctgcgcggca ccacgtgtat
    10561 gtaaggttgc cagcaccaga tgcccggttt ctgccgccgt cagcgccaga cggattgtct
    10621 cactgtcacg tagctctccg agcaaaatca catcaggatc ttcccgcaat gcagcccgca
    10681 accccgatgc gaacgtcata cagtgcaaac caatttcccg ctgctggatc aaacatcgct
    10741 ggctggcata gagatattcc acaggatctt ccagcgtcag aatatgcgca tcggcatgtt
    10801 gattaagata gccaaccatc gccgccagcg tggtagattt gccactcccc gtagcccccg
    10861 tcaccagaat caggccattc tcgctcttga gtaattccgg caataccggt ggcgcaccaa
    10921 gctgttcgag ctgtggacag tgcgaaggta acaaccgtaa tgccagagaa ataccttgcc
    10981 gttgcgcgaa cgcactgcca cgcaaccgct ggttttccgc cagcgacacg gcaaaatcca
    11041 gctggccatt ttccagcaat attgcccgct gatcgtcatc cagccactcc cgcagtagct
    11101 cttcgacgtc cggcgcatca aacggcgcag cttccattct gccacgaatg cgccatcgtg
    11161 cgggccaggc gctgcacagg tgtagatccg agacgttatg ctttacacta agggccacaa
    11221 tttcttccat attcatacta agatcctcgg aaaatgaacg atattgcgca taacctggca
    11281 caggtccggg acaaaatctc agcggctgca acgcgttgcg gccgttctcc agaagaaatt
    11341 acgctgcttg cagtcagtaa aacaaaacct gcgagcgcca tcgcagaagc cattgatgcc
    11401 gggcagcgtc aatttggtga aaactacgtt caggaagggg tagataaaat tcgccacttt
    11461 caggaactgg gcgtaacagg attagaatgg cattttattg gcccgttgca gtctaataaa
    11521 agccgcctag tggcagagca tttcgactgg tgtcatacca tcgaccgttt gcgcattgct
    11581 acccgcctca acgatcagcg ccctgcagaa cttccccctc ttaacgtact gattcaaatt
    11641 aatattagtg atgaaaacag taagtccggg attcaactgg cagaactgga cgagctggca
    11701 gcggcggtcg ctgaactacc gcgtttacgt ctgcgcgggc tgatggcaat ccctgcgcct
    11761 gagtcagaat atgtaaggca gtttgaagtt gcacgccaaa tggctgtagc atttgccgga
    11821 ctgaaaacgc gctacccgca tatcgacacg ctctctctgg gaatgtcgga cgatatggaa
    11881 gccgccattg cggcaggtag cacgatggtt cgtatcggca ctgcaatttt tggtgcgcgt
    11941 gattactcta aaaaataagg aattaaagga acgccatgaa tacgttgact ttcctgcttt
    12001 caacggtcat tgagctgtat accatggtgc tgttattacg catctggatg cagtgggctc
    12061 attgtgattt ttacaacccc ttctcacagt ttgtagtgaa ggtagcgcag ccaattatcg
    12121 ggccactgcg ccgcgttatt ccggcaatgg gtcccattga cagcgcctcg ctgctggttg
    12181 cctatattct cagttttatc aaagccattg tgctgtttga agtggtgacc ttcctgccaa
    12241 tcatctggat tgccggttta ctgattctgc tgaaaaccat cggcttgctg attttctggg
    12301 tcttgctggt gatggcgatt atgagctggg taagccaggg tcgtagcccg attgaatacg
    12361 tgctgattca gctggccgat ccgctgctgc gcccgattcg ccgcttgcta ccggcaatgg
    12421 gtgggattga tttctcgccg atgatcctcg ttctgctgct gtatgtcatc aatatgggtg
    12481 tcgcagaagt attacagtca accggaaaca tgctgctgcc ggggctgtgg atggcgttat
    12541 gagtgccgta acagttaatg atgacggtct ggttttacgg ctctatattc agccgaaagc
    12601 cagccgtgat tctattgtcg gtttacatgg cgacgaagtt aaagtcgcca ttaccgcgcc
    12661 gccggttgac ggccaggcca acagtcatct ggtgaagttt ctcggtaagc aattccgggt
    12721 tgccaaaagc caggtggtga ttgaaaaagg cgaacttggc cgccacaaac aaattaaaat
    12781 cattaatccg caacaaatcc cgccagaaat cgcggcgtta attaattagg tatcctatgc
    12841 aaaaagttgt cctcgcaacc ggcaatgccg gtaaagtgcg tgagctggcg tcgctgctta
    12901 gcgactttgg tcttgatatc gtggcccaaa cagacctcgg cgttgattcc gctgaagaaa
    12961 ccggcctaac ctttatcgaa aacgcgattc tgaaagcgcg ccatgcggca aaagtgaccg
    13021 gtttaccggc aattgccgac gactctggtc tggcggtaga tgcgctgggt ggcgcgccgg
    13081 gtatttactc cgcgcgttat tccggtgaag acgcgaccga tcaaaagaat ctgcaaaaac
    13141 tgttggaaac actgaaagac gtaccggacg accaacgtca ggcgcgtttc cactgcgtgc
    13201 tggtatatct gcgtcacgcg gaagatccga ctccgctggt atgccacggc agctggccgg
    13261 gcgtgattac tcgcgaacca gcgggcactg gtggctttgg ttatgatcca atcttcttcg
    13321 taccttccga agggaaaacc gccgccgaac tgacccgcga agaaaagagc gccatttccc
    13381 accgtggtca ggcgttgaaa ctgctgctgg acgctttacg taatggttaa attaccgccg
    13441 ctgagtctct acattcacat cccgtggtgc gtgcagaaat gcccgtactg cgatttcaac
    13501 tctcacgcgt tgaaaggaga agtgccgcac gacgattatg ttcagcatct gctttgcgat
    13561 ctggacaatg atgtggctta cgctcagggc cgtgaagtaa agacaatctt tattggcggt
    13621 ggtacgccga gcctgctttc cggcccggcg atgcaaacgc tgctggacgg cgtgcgtgcg
    13681 cgtttgccgc tggcagcgga tgcagaaatt actatggaag cgaacccagg cacggtagaa
    13741 gcggatcgct ttgtcgatta tcagcgtgct ggtgtaaacc gcatctctat tggtgtgcag
    13801 agttttagcg aagaaaagct gaaacgactt gggcgtattc atggcccgca agaagcgaaa
    13861 cgagcggcaa agctggcgag cggtttaggg ttacgtagct ttaaccttga tttgatgcat
    13921 ggactaccgg atcaatcact ggaagaggcg cttggcgatc tacgccaggc cattgaactg
    13981 aatccgccgc atctttcctg gtatcaactg accatcgaac ctaatacgct gtttggctcg
    14041 cgaccgccgg tgctgccgga cgatgacgcg ctgtgggata ttttcgaaca ggggcatcag
    14101 ttattaaccg cagcgggtta tcagcaatat gaaacttccg cttacgccaa acccggttat
    14161 cagtgccagc acaatctcaa ctactggcgc tttggtgact acatcggtat tggctgcggc
    14221 gcgcacggca aagtgacctt cccggatggg cgcattctgc gtaccactaa aacgcgtcat
    14281 cctcgtggtt ttatgcaagg aaggtatctg gaaagccagc gtgatgtcga agccgcagat
    14341 aagccgtttg agttctttat gaatcgcttc cgtctgctgg aggccgcgcc gcgcgtggag
    14401 tttattgcgt ataccgggct ttgcgaagat gtgattcgcc cacagttaga cgaggcgatt
    14461 gcccagggtt atctcaccga atgtgcggat tactggcaga taacggaaca tggtaaatta
    14521 ttcttaaact cacttcttga attattcctc gctgagtaat cattaatgcg cctgcgggcg
    14581 cattaatcac ttcttttatc cccccgttaa ttaacctcgg cagaaatata tttctta
//
LOCUS       DADLSE010000095        14605 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLSE010000095 DADLSE010000000
VERSION     DADLSE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.095"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(175..1368)
                     /locus_tag="JN692_002843"
     CDS             complement(175..1368)
                     /locus_tag="JN692_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265116.1"
                     /translation="MSPGIEDTPQKPQSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1504..3228
                     /gene="iucA"
                     /locus_tag="JN692_002844"
     CDS             1504..3228
                     /gene="iucA"
                     /locus_tag="JN692_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7265117.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3229..4176
                     /gene="iucB"
                     /locus_tag="JN692_002845"
     CDS             3229..4176
                     /gene="iucB"
                     /locus_tag="JN692_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7265118.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4176..5918
                     /gene="iucC"
                     /locus_tag="JN692_002846"
     CDS             4176..5918
                     /gene="iucC"
                     /locus_tag="JN692_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7265119.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            5915..7252
                     /gene="iucD"
                     /locus_tag="JN692_002847"
     CDS             5915..7252
                     /gene="iucD"
                     /locus_tag="JN692_002847"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7265120.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7258..9453
                     /gene="iutA"
                     /locus_tag="JN692_002848"
     CDS             7258..9453
                     /gene="iutA"
                     /locus_tag="JN692_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7265121.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10413..14300
                     /gene="sat"
                     /locus_tag="JN692_002849"
     CDS             10413..14300
                     /gene="sat"
                     /locus_tag="JN692_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7265122.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTANHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         3896 a         3499 c         3640 g         3570 t
ORIGIN      
        1 gatcataggt tgttgcacga aagaatcatg gagattacac cgcagcaact ggttgaattc
       61 actatacctg atgggcgggg gcagcaagcc agaacactgc ctgacatgct ttcatcagac
      121 gcaggtcctc tctcatatcc tctgatttac tccagtaagg tgatgatcat atctttattt
      181 gatagtacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      241 tgctgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      301 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      361 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      421 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      481 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      541 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      601 ttggctaatg gcggtggtat ccgtagcgaa ctggcgagta agggcaggcg aaagtccaag
      661 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
      721 tcgcttcaga ctgacgctct tgcactccgg tgtgggctgt ggtggtgtgc cgggcaaccg
      781 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
      841 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
      901 ggagctgatg gttgccagag cggccatgcg attcccttct ccagctctct gcaatgccca
      961 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1021 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1081 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1141 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1201 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1261 gagcacaacc agcagaccgt tctgtccgat accgagaagc ccggcactga acgcaagtgg
     1321 ccagcaggac tgtggttttt ggggcgtatc ttcgatccct ggtgacatta taatgtttca
     1381 actccatgta ttaattatgt ttatttgtaa aattaattta tctgacaata acattcattg
     1441 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1501 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1561 gcactgattc gtgaaaccac agactggaaa ctgacagaat acccgccaga cgaattgctc
     1621 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     1681 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     1741 tttaccactc tctcccggct tattattgat aagctacgcc atcaactgtt tctgcccgtt
     1801 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc atgtccatac gcaacaggca
     1861 attgatgccc gtcatgactg ggccgctctg cgtgaaaaag cgttgaattt tggcgaggct
     1921 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     1981 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2041 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2101 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2161 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2221 gcacttgttg ctaaagggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2281 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2341 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2401 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2461 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2521 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagact
     2581 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     2641 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     2701 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     2761 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     2821 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     2881 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     2941 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3001 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3061 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3121 aaaggtaact ttttctgtta tctgaacgat cataacgaga ataccattgt tgacccttcg
     3181 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3241 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3301 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3361 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3421 ccctgggccg aatggcgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3481 gacattatcc atcgtactgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3541 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3601 ccccgtccac agggtgaggt ttaccgccgt tatgatccac gtattcgcag gatgctgagt
     3661 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     3721 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     3781 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     3841 agctatttcg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     3901 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     3961 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4021 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4081 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4141 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4201 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4261 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttca
     4321 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4381 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4441 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4501 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4561 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4621 gcaaagaggc gctggaacga tatgcgccag agtataccaa caccttcaga ctgcactggc
     4681 tggcggtaaa acgtgaacat atgatctggc gctgcgataa cgatctggat attcagcagt
     4741 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     4801 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     4861 tcgccacaga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     4921 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     4981 ggctggatat caagctgccg ctgaccatct acaatacctc atgctaccgg gggatccctg
     5041 gcagatatat cgctgccgga ccactagctt cacgctggct gcaacaggtt tttgcgaccg
     5101 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5161 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5221 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtcct gttctgatgg
     5281 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5341 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5401 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5461 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5521 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5581 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     5641 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     5701 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatggca gagcgttttg
     5761 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     5821 cctggccgga cctggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     5881 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     5941 tgtcgggaca gggccattta atctcagcat tgctgcattg tcacatcaga tcgaagaact
     6001 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6061 ggattgtcat atgcagaccg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6121 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6181 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6241 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6301 attgtttctg gttcagacca gtcggggaga atattttgcc cacaatatct gccttggtac
     6361 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6421 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataaccg tggttggtgg
     6481 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6541 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6601 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     6661 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     6721 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     6781 tctgctaccc agtcgctcgg taacaactct ggaaagtagt ggtccgggct ggaggttatt
     6841 gatggaacat catctggatc agggcagaga gagcctggaa agtgatgtgg tgattttcgc
     6901 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     6961 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7021 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7081 gctcagcctg atggcctggc gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7141 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7201 gcagccggag gctgcttctt taactcacta tacagcaaat attcaggaat aacgatgatg
     7261 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7321 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7381 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7441 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7501 agccagagtc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7561 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7621 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     7681 accggagggt tgatcaacat cgtgaccaaa aaaggtcagc cggaaaccat gatggagttt
     7741 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     7801 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     7861 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     7921 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     7981 cagcttcaac tgataacgca gtactataaa agtcagggag acgacaatta cgggcttaat
     8041 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8101 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8161 gatttcctgg gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggtac
     8221 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8281 gataccgacc agtacggcat gaaactgact ctgaacagcc aactgatgga cggctggcaa
     8341 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8401 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8461 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8521 gatattttta ccgttagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8581 ttcatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     8641 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     8701 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     8761 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     8821 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     8881 ggctggcgct ttatcggtga caacctgcgg actcaaatcg cggcatatta ctcgctttcc
     8941 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9001 atttacggcg tggaaggtgc ggtggactac ttgatcccgg atactgactg gagtaccggt
     9061 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9121 gtgaaggaat caagtccatc gaaagcgaca gcttacatta actgggcgcc ggaaccgtgg
     9181 agtctgcgtg tacagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9241 aatggttaca ctaccgtcga ttttatcagt agttggcagc ttccggtggg aacactcagc
     9301 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9361 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9421 acctttggtc tgaactactc agtgctgttc tgacggggat gcttttgcaa caagggtatg
     9481 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtattgt ttacagccag
     9541 tataatatac agccttaaca agatattccg tctccggaat ggactgttca ccactgaata
     9601 acattatgtc tgttttatgg atctttgact gaaatttatc gcatatcttc atttcattgt
     9661 ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc agatcctgaa
     9721 attcctgaga ggatgatctg cgcccaatat cctgagccgg aactttgctg caatgaaggc
     9781 caacgaaaaa tgggggagta tgttatagag tacttgctac agggtaaaaa gaggtacctg
     9841 tcgctggttc tccgttcagt ttaaccggga gattttctcc tgcgcctgtc ataaagtccg
     9901 gtaatggaga ttgttaatac gatggtgcac gatgcattgt cagtgcccgg cccggaggat
     9961 gcaccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta taggcaaagt
    10021 taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg aataacgcag
    10081 tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc taattcgtcg
    10141 tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa tattaaacat
    10201 tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattaacga tcaattagta
    10261 ttgattcaca taagctctca tcattaatga gaacattgtc acatatttac aatatagaac
    10321 tgttactttt agcaagctgt tagtaaccaa ccgttactaa taattttaat tcttaatact
    10381 ttgttcattc aataaatgag ttgagagaat atatgaataa aatatactcc cttaaatata
    10441 gtgctgccac tggcggactc attgctgttt ctgaattagc gaaaagagtt tctggtaaaa
    10501 caaaccgaaa acttgtagca acaatgttgt ctctggctgt tgcaggtaca gtaaatgcag
    10561 caaatattga tatatcaaat gtatgggcga gagactatct tgatcttgca caaaataaag
    10621 gtattttcca gcccggagca acagacgtaa caatcacttt aaaaaacgga gataaattct
    10681 ctttccataa tctctcaatt ccggattttt ctggtgcagc agcgagtggc gcagctaccg
    10741 caataggagg ttcttatagt gttactgttg cacataacaa aaagaaccct caggccgcag
    10801 aaactcaggt ttacgctcag tcttcttaca aggttgttga cagaagaaat tccaatgatt
    10861 ttgagattca gaggttaaat aaatttgttg tggaaacagt aggtgccacc ccggcagaga
    10921 ccaaccctac aacatattct gatgcattag aacgctacgg tatagtcact tctgacggtt
    10981 caaaaaaaat cataggtttt cgtgctggct ctggaggaac atcatttatt aatggtgaat
    11041 ccaaaatctc aacaaattca gcatatagcc atgatctgtt aagtgctagt ctatttgagg
    11101 tcacccaatg ggactcatac ggcatgatga tttataaaaa tgataaaaca tttcgtaatc
    11161 ttgaaatatt cggagacagc ggctctggag catacttata tgataacaaa ctagaaaaat
    11221 gggtattagt cggaacaacc catggtattg ccagcgttaa tggtgaccaa ctgacatgga
    11281 taacaaaata caatgataaa ctggttagtg agttaaaaga tacctatagt cataaaataa
    11341 atctgaatgg caataatgta accattaaaa acacagatat aacattacac caaaacaatg
    11401 cagataccac tggtactcaa gaaaaaataa ctaaagacaa agatattgtg ttcacaaatg
    11461 ggggaaatgt tctgtttaag gataatttgg attttggtag cggtggtatt atctttgacg
    11521 aaggccatga atataacata aacggtcagg gatttacatt taaaggagca ggaattgata
    11581 tcggaaaaga aagcattgta aactggaatg cattgtattc cagtgatgat gttttacaca
    11641 aaataggccc cggtactctg aatgttcaaa aaaaacaggg ggcaaatata aagataggtg
    11701 aaggaaatgt tattcttaat gaagaaggaa catttaacaa tatatacctt gcaagcggaa
    11761 atggtaaggt aatactaaat aaagataatt cccttggcaa tgatcaatat gcggggatat
    11821 tttttactaa acgtggtggc acgctagatt taaatggaca caatcagact tttactagaa
    11881 ttgccgccac tgacgatgga acaacaataa ctaactcaga tacaacgaaa gaagccgttc
    11941 tggcaatcaa taacgaagac tcctacatat atcatgggaa cataaatggc aatataaaac
    12001 taacgcacaa tattaattct caggataaga aaactaatgc aaaattaatt ctggatggta
    12061 gtgtcaacac aaaaaatgat gttgaagtca gtaatgccag tcttaccatg caaggccatg
    12121 caacagagca tgcaatattc agaagcacag cgaatcattg ctccctggta tttctttgtg
    12181 gaacggactg ggtcaccgtt ttgaaagaaa cagagagttc atataataaa aagttcaatt
    12241 ctgattacaa aagtaataat cagcagacct catttgatca gcctgactgg aaaaccgggg
    12301 tgtttaaatt tgatacatta cacctgaaca atgctgactt ttcaatatca cgcaatgcca
    12361 atgttgaagg aaatatatca gcaaataaat cagctatcac aatcggcgat aaaaatgctt
    12421 acattgataa tcttgcaggg aaaaatatta ctaataatgg ttttgacttc aaacaaacta
    12481 tcagtactaa tctatccata ggagaaacta aatttacagg tggcatcact gcacataaca
    12541 gccaaatagc cataggtgat caagctgtag ttacacttaa tggtgcaacc tttctgaata
    12601 atactcctat aagtatagat aaaggagcaa aagttatagc acaaaattcc atgttcacaa
    12661 caaaaggtat tgatatctcc ggtgaactga ctatgatggg aatccctgaa cagaacagta
    12721 aaactgtaac gccgggtctc cactacgctg ctgatggatt caggctgagt ggtggaaatg
    12781 caaatttcat tgccagaaat atggcatctg tcaccggaaa tatttatgct gatgatgcag
    12841 caaccattac tctgggacag cctgaaactg aaacaccgac tatatcgtct gcttatcagg
    12901 catgggcaga gactcttttg tatggctttg ataccgccta tcgaggcgca ataacagccc
    12961 ccaaagctac agttagcatg aataatgcga tctggcatct aaatagccag tcatcaatta
    13021 atcgtctaga aacaaaagac agtatggtgc gttttactgg tgacaatggg aagtttacaa
    13081 cccttacagt ggacaacctt actatagatg acagtgcatt tgtgctgcgt gcaaatctgg
    13141 cccaagcaga tcagcttgtt gtcaataaat cgttgtctgg taaaaacaac cttctgttag
    13201 tcgacttcat tgagaaaaat ggaaacagca acggactgaa tatcgatctg gtcagcgcac
    13261 caaaaggaac tgcagtagat gtctttaaag ctacgactcg gagtattggc ttcagtgatg
    13321 taacaccggt tatcgagcaa aagaacgata cagacaaagc aacatggact ctgatcggct
    13381 ataaatctgt ggccaacgcc gatgcggcta aaaaggcaac attactgatg tcaggcggct
    13441 ataaagcctt ccttgctgag gtcaacaacc ttaacaaacg tatgggtgat ctgcgtgaca
    13501 ttaacggtga gtccggtgca tgggcccgaa tcatgagcgg aaccgggtct gccggcggtg
    13561 gattcagtga caactacacc cacgttcagg tcggtgcgga taacaaacat gaactcgatg
    13621 gccttgacct cttcaccggg gtgaccatga cctataccga cagccatgca ggcagtgatg
    13681 ccttcagtgg tgaaacgaag tctgtgggtg ccggtctcta tgcctctgcc atgtttgagt
    13741 ccggagcata tatcgacctc atcggtaagt acgttcacca tgacaacgag tataccgcaa
    13801 ctttcgccgg ccttggcacc agagactaca gctcccactc ctggtatgcc ggtgcggaag
    13861 tcggttaccg ttaccatgta actgactctg catggattga gccgcaggcg gaacttgttt
    13921 acggtgctgt atccgggaaa cagttctcct ggaaggacca gggaatgaac ctcaccatga
    13981 aggataagga ctttaatccg ctgattgggc gtaccggtgt tgatgtgggt aaatccttct
    14041 ccggtaagga ctggaaagtc acagcccgcg ccggccttgg ctaccagttt gacctgtttg
    14101 ccaacggtga aaccgtactg cgtgatgcgt ccggtgagaa acgtatcaaa ggtgaaaaag
    14161 acggtcgtat gctcatgaat gttggtctca atgccgaaat tcgcgataat cttcgcttcg
    14221 gtcttgagtt tgagaaatcg gcatttggta aatacaacgt ggataacgcg atcaacgcca
    14281 acttccgtta ctctttctga taacagcccg ggccgcgttt gcggcccttc ttctaccgga
    14341 gagaatatgt attaccctgt gacagactat atcgctcttg ctctcattat tagctttctt
    14401 tttctgacat tatttatctg cctgttatgc cttaaacatg agcgaataaa aaaagaaact
    14461 atcaggcaaa aaaatgcaca tattctggat catggctgga atgcaactga gttctcatgg
    14521 ttccgatacg gacagtataa cgaaacgggt atttacatct cgatcgagaa gaccattatc
    14581 attacaatca ccgtttctgg tgggt
//
LOCUS       DADLSE010000096        14336 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLSE010000096 DADLSE010000000
VERSION     DADLSE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.096"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN692_002850"
     CDS             complement(93..218)
                     /gene="ecnA"
                     /locus_tag="JN692_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7265123.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(270..836)
                     /gene="efp"
                     /locus_tag="JN692_002851"
     CDS             complement(270..836)
                     /gene="efp"
                     /locus_tag="JN692_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7265124.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            878..1906
                     /gene="epmB"
                     /locus_tag="JN692_002852"
     CDS             878..1906
                     /gene="epmB"
                     /locus_tag="JN692_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7265125.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDKLLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2301..3170
                     /locus_tag="JN692_002853"
     CDS             2301..3170
                     /locus_tag="JN692_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265126.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(3373..3726)
                     /locus_tag="JN692_002854"
     CDS             complement(3373..3726)
                     /locus_tag="JN692_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7265127.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN692_002855"
     CDS             complement(3864..5519)
                     /gene="groL"
                     /locus_tag="JN692_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010427772.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7265128.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMGGMM"
     gene            complement(5563..5856)
                     /locus_tag="JN692_002856"
     CDS             complement(5563..5856)
                     /locus_tag="JN692_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7265129.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            6132..7388
                     /gene="yjeH"
                     /locus_tag="JN692_002857"
     CDS             6132..7388
                     /gene="yjeH"
                     /locus_tag="JN692_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7265130.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN692_002858"
     CDS             complement(7404..7880)
                     /gene="fxsA"
                     /locus_tag="JN692_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7265131.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            8217..9653
                     /gene="aspA"
                     /locus_tag="JN692_002859"
     CDS             8217..9653
                     /gene="aspA"
                     /locus_tag="JN692_002859"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7265132.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            9771..11072
                     /locus_tag="JN692_002860"
     CDS             9771..11072
                     /locus_tag="JN692_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7265133.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            11188..11526
                     /gene="cutA"
                     /locus_tag="JN692_002861"
     CDS             11188..11526
                     /gene="cutA"
                     /locus_tag="JN692_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7265134.1"
                     /translation="MLDEKSSNTASVVVLCTAPYEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            11502..13199
                     /locus_tag="JN692_002862"
     CDS             11502..13199
                     /locus_tag="JN692_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7265135.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            13236..13811
                     /locus_tag="JN692_002863"
     CDS             13236..13811
                     /locus_tag="JN692_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7265136.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            13918..13993
                     /locus_tag="JN692_002864"
     tRNA            13918..13993
                     /locus_tag="JN692_002864"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13951..13953,aa:Phe,seq:gaa)
BASE COUNT         3492 a         3676 c         3524 g         3644 t
ORIGIN      
        1 aatgttattt atgtttgcct atagcaaatt aataatagac aacatcgcca aaaatgatgg
       61 aaatctgatg atttttcgga agagaagaaa aattagctgg cagcgcgaga gatggagttg
      121 ccgagatgtt tgatgtcttc gccgaaaccg caagcggtgt tacagcccgt gagcagcgtg
      181 ctggcaagca aaacaagaac gataaggcgt ttcatcatct ctgacctgaa taagtgatgg
      241 tgcagcctgc aggccgcacc acaaccgcat tacttcacgc gagagacgta ttcaccagag
      301 cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca gcggaacttt aaccacagcg
      361 ccagtagaca gggtagccgg tttgccgcca gtacctgcgg tatcaccttt caggcccgga
      421 tcggtatcaa cgatttccag ttcaacgaag ttcggcggag taacggagat cggctgacca
      481 ttccacagag ttacgataca ctctgcctga tccagcagcc atttagcgtt gtcgccaatt
      541 gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt tcatgaagtg ccagaactca
      601 ccgtcgttgt acaggtaagt caggttcata tcgacaacat cagcgccttc agcggaatca
      661 gtagatttga aggttttttc tacgcgagta ccggtcagca gacgacgcag tttaacgcga
      721 gcaaatgcct ggcctttacc cggttttacg aattcactcg cttcaaccgc gtaaggttcg
      781 ccgtctaaca tgattttaag accagcacga aaatcgttgc tatagtacgt tgccataagg
      841 ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg gcgcatattg taaccctaaa
      901 taccccatcc agagaagatt ggttaacgca acttgccgat gttgtgaccg atcctgataa
      961 acttctgcgt cttttgaata tagacgcgga cgaaaaactg ttggccggac gcagcgccaa
     1021 aaagctgttt gccctgcgtg tgccccgctc atttatcgat cgcatggaga aaggcaatcc
     1081 ggacgatcct cttttgcgtc aggtacttac ctcgcaagat gagtttgtcg tcgcgtccgg
     1141 attctccacc gacccactgg aagaacagca cagcgtagtg cctggtttgt tgcataaata
     1201 ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg gtaaattgcc gctattgctt
     1261 ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag cgtaactggc agactgcact
     1321 tgagtatgtt gctgcgcatc cggaactgga cgagatgatt ttctccggcg gcgatccgct
     1381 gatggcgaaa gatcacgagc tggactggtt gctcacacaa ctggaagcca tcccgcatat
     1441 caaacgtctg cggattcaca gccgtctgcc gattgtgatc ccggcacgta tcaccgaggc
     1501 gctggttgaa cgctttgccc gttctacgct gcaaatcttg ctggtgaatc acatcaatca
     1561 tgccaatgag gtagatgaaa cgttccgtca ggcgatggct aaattgcgcc gtgtcggtgt
     1621 caccctgctt aaccagagcg ttctgttacg tggtgtgaac gataacgcac aaacgttggc
     1681 aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg tattacctgc atgtgctaga
     1741 taaagtacag ggcgcggcgc attttatggt gagtgatgac gaagcacggc agattatgcg
     1801 tgagttgctg acattggtgt cgggatatct ggtgccgaaa ctggcgcgag aaattggcgg
     1861 cgaacccagc aaaacgccgc tggatctcca gctacgccag cagtaactga ttaaacaggg
     1921 aagttaaaca ttaaaattgc ctgatgcgct acgcttatca ggcctacatc gtgcattgaa
     1981 attgaatgga tttgcaatga actgtaggcc ggataaggcg tttacgccgc atccggcaag
     2041 agcaacaaac gcaagtacag caacctgtca ttaaactgag aaatttaatc tttgcacgtt
     2101 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     2161 taattaacct ttcttttctg tttatataga gtgagcacgg ctatttatgt tattttaaca
     2221 actattacta ccaagcccat ttatctggct tataaattaa aattaattat ttaattgaga
     2281 aatggttagg gagaacctac atggccataa gcatcaaggg tgttaatacc ggtgttattc
     2341 gcaagagtaa taatttcatt gcactggcgc tgaaaattaa agaacctcgc aataaagaat
     2401 cgttgttctt tatgtctgtc atggaactgc gcgacctgct gattgcgctg gaaagtcgtc
     2461 tgcatcaaaa gcataaactg gatgctgctg cacgccttca gtatgagcaa gctcgcgata
     2521 aagtcattaa gaaaatggca gaaaacatcc cagaaattct ggttgatgag cttaaaaatg
     2581 ccgatattaa tcgtcgggtt aacacgctgg aacttaccga taatcaaggc gaaaatctta
     2641 ccttcgtatt aacgctacat gacggtagca aatgtgagtt agtcgtcaat gagttgcaga
     2701 ttgaaatgct ggcgcgagca atcattcatg caattaataa tgcggaaatg cgtgaactgg
     2761 cattacgtat tacctcactg ttagatttcc tgccgttata cgacgttgac tgccaggaca
     2821 atggcaatct ggaatatgac acctattccc aaccggaatg gaaacataac ttgttcgacc
     2881 attatctggc ggtgctttac cgctttaaag atgaaagtgg caaagaacaa tttagcggcg
     2941 cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa
     3001 tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc
     3061 gcaccgtagc ggcgaataac gcccaaccgc taacccagga tcaatgctta cgcgcgctgc
     3121 atcacttacg tgtacaatcc accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt
     3181 gctaatgcga ggctggagcc atccagcctg aaatgactga taacgtgatc gttggtcatg
     3241 ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct caagcattca atcagttgca
     3301 atgcctgctg taggcctgat aagcgaagcg catcaggcaa taatacgttt gtcacccagc
     3361 ctctggatca catcagttcg ggcacttata aacctgaccg ataatctggc tatccgtcgg
     3421 gacaaaactg gacaacattc cctgcgacgg gctgctgatg ccataaatca cgttaccgcc
     3481 cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg cgcatagaac cgccctcttc
     3541 tccgtgttgc ccggaaagcc agttgctttg cttacctgtc gcagtaccaa tcagctggca
     3601 ctctgcgcct ggctgctcgt ccacaatgcg tacactctga ccggcagcac tcaattcgtt
     3661 gctggagcta caacccgcca tcagtagcgc ggcaccgaca atccctgcta agtattttac
     3721 gtgcatgtta ttccccataa tcaatgagct ggacgccact cgcgtcgtcc gtgtctgaat
     3781 cttatactag aaagatgacc aaaagaaaaa cccccagaca tttctgcccg ggggtttgtt
     3841 tatttctgcg aagtgcaggg caattacatc atgccgccca tgccacccat gccgcccata
     3901 ccgcccatac cgccagcagc gcctaagtca gctgcatcgt ttttcggcag gtcggtaacc
     3961 atgcattcgg tggtgatcat caggccagcc acagaagctg cgtactgcag agcagaacgg
     4021 gttactttgg ttgggtccag gatacccatg tcgatcatgt tgccgtattc ttcggttgct
     4081 gcgttgtaac cgtagttgcc gtcgccgcct ttaacggtgt tagcaacaac agacggttct
     4141 tcgccgcagt tcaggacgat ctgacgcagc ggagcttcca ttgcacgcag tgcaactttg
     4201 atacccacgt tctggtcttc gttctgacca cgcaggtcag ccagtttaga cgctacgcgg
     4261 atcagcgcaa caccaccacc agcaaccacg ccttcttcta ccgcagcacg ggtcgcgtgc
     4321 agggcatctt caacgcgtgc ttttttctct ttcatttcaa cttcggtagc agcacctact
     4381 ttgataactg caacgccgcc tgccagtttc gctacgcgct cctgcagttt ttcacggtcg
     4441 tagtcagaag ttgcttcttc aatctgctga cggatctgag caacacggcc ctggattgca
     4501 gcttcttcac ccacgccatc gatgatagtg gtggtgtctt tgttgatcac aacacgttta
     4561 gcctgaccca ggtcttccag ggttgctttt tccagctcca taccgatctc ttcagagatt
     4621 acggtaccgc cagtcagggt tgcgatatcc tgcagcatag ctttacgacg atcgccgaag
     4681 cccggagctt taaccgcagc aactttcacg atgccacgca tggtgttaac aaccagagtt
     4741 gccagcgctt cgccttcaac atcttcagcg atgatcagca gcggtttgcc tgctttggca
     4801 acagcttcca gaaccggcag catttcgcgg atgttggaga ttttcttgtc agccagcagg
     4861 atgaacgggc tttccagttc tactgcgcca gtttccggct tgttgatgaa gtaaggagac
     4921 aggtagccac ggtcgaactg cataccttca accacgtcca gttcgtcctg cagaccggta
     4981 ccgtcttcaa cggtgataac gccttcttta ccgactttgt ccatcgcttc agcgatcagt
     5041 ttacctacgg tttcgtcgga gttagcggag atggtaccaa cctgagcaat cgctttagaa
     5101 tcagagcacg gtacggacag cgctttcagt tcttcaactg cagcggtaac cgctttgtcg
     5161 ataccacgtt tcaggtccat cgggttcatg cccgcagcaa cagctttcag accttcagtg
     5221 atgatagcct gagccagtac ggttgcagtg gtggtaccgt cgcctgcagc gtcgttcgct
     5281 ttggaggcaa cttctttcac catctgcgca cccatatttt cgaacttgtc ttccagttcg
     5341 atttcacgag caacggaaac accatctttg gtgatggtcg gtgcaccgaa agatttatcc
     5401 agaactacgt tacggccttt tggaccgagg gtaactttca ctgcatctgc cagtacgttt
     5461 acgccgcgca gcattttcac acgagcgtcg ttaccgaatt ttacgtcttt agctgccatt
     5521 atctttattc cttaaattcg tatgttctgt gtcgtgcgcg gattacgctt caacaattgc
     5581 cagaatgtcg ctttcggaca tgatcaacac ttcttcattg tcgatcttct cggatttcac
     5641 accgtagcca tcgttgaaaa taacgatgtc gccaactttc acgtccagcg gcttcacttc
     5701 gccattttca aggatacggc cattgccgac agccagcact tcgccgcggg tggatttagc
     5761 cgctgcagag ccggtcagaa cgatgccgcc agcagattta gtttcaactt ctttacgctt
     5821 gacgatcacg cgatcatgca atggacgaat attcattgat aactctcctt tgagaaagtc
     5881 cgtatctgtt atgggtgacg ccggagctta cgtggtttcc cggctggtga ccagagaaat
     5941 ggggatgagg cttcgcccct tcaaggggga aaaagaaaaa aaattctgat cagcacaaaa
     6001 tcgggtgaaa accctgattc acctcacatt tcatcacaat ttcttcatcg ccgtcataag
     6061 cgaatctgat tgtgctacca tcgaaaatct acgcagttgc ccaaaatttg ggcgcaatcg
     6121 gatatcaacc catgagtgga ctcaaacaag aactggggct ggcccagggc attggcctgc
     6181 tatcgacgtc attattaggc actggcgtgt ttgccgttcc tgcgttagct gcactggtag
     6241 caggcaataa cagcctgtgg gcgtggcccg ttttgattat cttagtgttc ccgattgcga
     6301 ttgtgtttgc gattctgggt cgccactatc ccagcgcagg cggcgtcgcg cacttcgtcg
     6361 gtatggcgtt tggttcgcgg cttgagcgag tcaccggctg gctgttttta tcggtcattc
     6421 ccgtgggttt gcctgccgca ctacaaattg ccgccggatt cggccaggca atgtttggct
     6481 ggcatagctg gcaactgttg ttggcagaac tcggtacgct ggcgttggtg tggtatatcg
     6541 gtactctcgg tgccagttcc agtgctaatc tacaaaccgt tattgccgga cttatcgtcg
     6601 cactgattgt cgctatctgg tgggcgggcg atatcaaacc tgcgagtatc cccttccccg
     6661 cgccaggaaa tatcgaactt accgggttat ttgctgcgtt atcagtgatg ttctggtgtt
     6721 ttgtcggtct ggaagcattt gcccatcttg cctcggaatt taaaaatcca gagcgtgatt
     6781 ttcctcgtgc tttgatgatt ggtctgctgc tggcaggatt agtctactgg ggctgtacgg
     6841 tagtcgtctt acacttcgac gcctatggtg aacaaatggc ggcggcagca tcgcttccaa
     6901 aaattgtagt gcagttgttc ggtgtaggag cgttatggat tgcctgcgta attggctatc
     6961 tggcctgctt tgccagtctt aacatttata tacagagctt cgcccgcttg gtctggtcgc
     7021 aggcgcaaca taatcctgac cactacctgg cacgcctctc ttctcgccat atcccgaata
     7081 atgccctcaa tgcggtgctt ggctgctgcg tggtgagcac gttggtaatt catgccttag
     7141 agatcaatct ggacgctctt attatttatg ccaatggcat ctttattatg atttatctgt
     7201 tatgcatgct ggcaggctgt aaattattgc aaggtcgtta tcgactactg gcggtggttg
     7261 gcgggctgtt atgcgttctg ttactggcaa tggtcggctg gaaaagtctc tatgcgctga
     7321 tcatgctggt ggggttatgg ctgtttctgc caaaacgaaa aacgccggaa aatggcataa
     7381 ccacataatc cggcgtttcg acattaatcc tggcgatcgt ctttatgatc aaggcggtcg
     7441 cggtcatcat cctttcgctg atactcacca tcaaaagtat taccgccacc ggtcccggcg
     7501 ctaaaaccgc cgcctggcat gcgagaaaag cgcaaatgtg gcatcaactt cactgtcaga
     7561 tgcttttgca ccggcggcaa taaaagcaga agaccgagga agtcggtgaa aaagcccggt
     7621 aataaaagca gcaaaccggc aataatcagc gaaacacttt taatcatctc cgccgcaggg
     7681 ttttcacccg ccgccatttt ttgctgcatc agcacgaaat tcttaaagcc ctggttacgt
     7741 accagtgaca taccgataac tgacgtgaat ataaccagca cgagggtcag caataccccc
     7801 aatacatggg caacctgaat aaagattgaa atctcaatat agacataaag gaaaatggca
     7861 ataaaaggta accagcgcaa aggtttctcc tgtaatagca gccggttaaa cccggctacc
     7921 tgaatgggtt gcgaatcgcg ttcagcttat attgtggtca ttagcaaaat ttcaagatgt
     7981 ttgcgcaact atttttggta gtaatcccaa agcggtgatc tatttcacaa attaataatt
     8041 aaggggtaaa aaccgacact taaagtgatc cagattacgg tagaaatcct caagcagcat
     8101 atgatctcgg gtattcggtc gatgcagggg ataatcgtcg gtcgaaaaac attcgaaacc
     8161 acatatattc tgtgtgttta aagcaaatca ttggcagctt gaaaaagaag gttcacatgt
     8221 caaacaacat tcgtatcgaa gaagatctgt tgggtaccag ggaagttcca gctgatgcct
     8281 actatggtgt tcacactctg agagcgattg aaaacttcta tatcagcaac aacaaaatca
     8341 gtgatattcc tgaatttgtt cgcggtatgg taatggttaa aaaagccgca gctatggcaa
     8401 acaaagagct gcaaaccatt cctaaaagtg tagcgaatgc catcattgcc gcatgtgatg
     8461 aagtcctgaa caacggaaaa tgcatggatc agttcccggt agacgtctac cagggcggcg
     8521 caggtacttc cgtaaacatg aacaccaacg aagtgctggc caatatcggt ctggaactga
     8581 tgggtcacca gaaaggtgaa tatcagtacc tgaacccgaa cgaccatgtt aacaaatgtc
     8641 agtccactaa cgacgcctac ccgaccggtt tccgtatcgc agtttactct tccctgatta
     8701 agctggtaga tgcgattaac caactgcgtg aaggctttga acgtaaagct gtcgaattcc
     8761 aggacatcct gaaaatgggt cgtacccagc tgcaggacgc agtaccgatg accctcggtc
     8821 aggaattccg cgctttcagc atcctgctga aagaagaagt gaaaaacatc caacgtaccg
     8881 ctgaactgct gctggaagtt aaccttggcg caacagcaat cggtactggt ctgaacacgc
     8941 cgaaagagta ctctccgctg gcagtgaaaa aactggctga agtcactggc ttcccatgcg
     9001 taccggctga agacctgatc gaagcgacct ctgactgcgg cgcttatgtt atggttcacg
     9061 gcgcgctgaa acgcctggct gtgaagatgt ccaaaatctg taacgacctg cgcttgctct
     9121 cttctggtcc acgtgccggc ctgaacgaga tcaacctgcc ggaactgcag gcgggctctt
     9181 ccatcatgcc agctaaagta aacccggttg ttccggaagt ggttaaccag gtatgcttca
     9241 aagtcatcgg taacgacacc actgttacca tggcagcaga agcaggtcag ctgcagttga
     9301 acgttatgga accggtcatt ggccaggcca tgttcgaatc cgttcacatt ctgaccaacg
     9361 cttgctacaa cctgctggaa aaatgcatta acggcatcac tgctaacaaa gaagtgtgcg
     9421 aaggttacgt ttacaactct atcggtatcg ttacttacct gaacccgttc atcggtcacc
     9481 acaacggtga catcgtgggt aaaatctgtg ccgaaaccgg taagagtgta cgtgaagtcg
     9541 ttctggaacg cggtctgttg actgaagcgg aacttgacga tattttctcc gtacagaatc
     9601 tgatgcaccc ggcttacaaa gcaaaacgct atactgatga aagcgaacag taatcgtaca
     9661 gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt gagcagtaac
     9721 ttaaaaataa caatctaata tcaacttgtt aaaaaacaag gaaggctaat atgctagttg
     9781 tagaactcat catagttttg ctggcgatct tcttgggcgc cagattgggg ggaataggta
     9841 ttggttttgc aggcggattg ggggtgctgg ttcttgccgc tattggcgtt aaacccggta
     9901 acatcccgtt cgatgtcatt tccattatca tggcggttat cgccgctatt tctgccatgc
     9961 aggttgctgg cggtctggac tatctggttc atcagacaga aaagctgctg cgccgtaacc
    10021 cgaaatacat cacgatcctc gcaccgatcg tgacctattt cctgactatc tttgctggta
    10081 ctggcaacat ctctctggcg acactgccag ttatcgctga agttgcgaag gaacaaggcg
    10141 ttaaaccttg ccgtccgctc tctactgcag tggtatccgc gcagattgcg atcaccgcat
    10201 cgccaatctc agcggcagtg gtttacatgt cttccgtgat ggaaggtcat ggcatcagct
    10261 acctccatct gctctccgtg gtcatcccgt ccaccctgct ggcggttctg gtgatgtcct
    10321 tcctggtcac tatgctgttc aactccaaac tctctgacga tccgatttat cgcaagcgtc
    10381 tggaagaggg cttggttgaa ctgcgcggtg aaaagcagat tgaaatcaaa tccggtgcaa
    10441 aaacgtccgt ctggctgttc ctgctgggcg tagttggcgt ggttatctat gcaatcatca
    10501 acagcccaag catgggtctg gttgaaaaac cgctgatgaa caccaccaac gcaatcctga
    10561 tcatcatgct cagcgttgca actctgacca ccgttatctg taaagtcgat accgacaaca
    10621 tcctcaactc cagcaccttc aaagcaggta tgagcgcctg tatttgtatc ctgggtgttg
    10681 cgtggctggg cgatactttc gtttccaaca acatcgactg gatcaaagat accgctggtg
    10741 aagtgattca gggtcatccg tggctgctgg ccgtcatctt cttctttgct tctgctctgc
    10801 tgtactctca ggctgcaacc gcaaaagcac tgatgccgat ggctctggca ctgaacgttt
    10861 caccgctgac cgctgttgct tctttcgctg cggtgtctgg tctgttcatt ctgccgacct
    10921 acccgacgct ggttgctgcg gtacagatgg atgacacggg tactacccgt atcggtaaat
    10981 tcgtcttcaa ccatccgttc ttcatcccgg gtactctggg tgttgccctg gccgtttgct
    11041 tcggcttcgt gctgggtagc ttcatgctgt aatgatcaat cgcggggcgt tcacgccccg
    11101 ctttctttcc cgccgactaa catccttccc ccgtccgttg tatagtgacc tctctcttgc
    11161 ggttccatct gttcttgcga ggtgtttatg cttgatgaaa aaagttcgaa taccgcgtct
    11221 gtcgtggtgc tatgtaccgc accgtatgaa gcgacagccc aggatttagc cgccaaagtg
    11281 ctggcggaaa aactggcggc ctgcgcgacc ttgatccccg gcgctacctc tctctattac
    11341 tgggaaggta agctggagca agaatacgaa gtgcagatga ttttaaaaac taccgtatct
    11401 caccagcagg cactgctgga atgcctgaag tctcatcatc catatcaaac cccggaactt
    11461 ctggttttac ctgttacaca cggagacaca gattacctct catggctcaa cgcatcttta
    11521 cgctgattct gctactttgc agcacttccg tttttgccgg attattcgac gcgccgggac
    11581 gttcacaatt tgtccccgcg gatcaagcct ttgcttttga ttttcagcaa aaccaacatg
    11641 accttaatct gacctggcag atcaaagacg gttactacct ctaccgtaaa cagatccgca
    11701 ttacgccgga acacgcgaaa attgccgacg tgcagctgcc gcaaggcgtc tggcatgaag
    11761 atgagtttta cggcaaaagc gagatttacc gcgatcggct gacgcttccc gtaaccatca
    11821 accaggcgag tgcgggagcg acgttaactg tcacctacca gggctgtgct gatgccggtt
    11881 tctgttatcc gccagaaacc aaaaccgttc cgttaagcga agtggtcgcc aacaacgcag
    11941 cgtcacagcc tgtgtctgtt tcgcagcaag agcagcacac cgcgcaattg cccttttccg
    12001 cgctctgggc gttgttgatc ggtattggta tcgcctttac gccatgcgtg ctgccaatgt
    12061 acccactgat ttctggcatc gtgctgggtg gtaaacagcg gctctccact gccagagcat
    12121 tgttgctgac ctttatttat gtgcagggga tggcactgac ttacacggcg ctgggtctgg
    12181 tagttgccgc cgcagggtta cagttccagg cggcgctaca gcacccatac gtgctcattg
    12241 gcctcgccat cgtctttacc ttgctggcga tgtcaatgtt tggcttgttt actctacaac
    12301 tcccctcttc gctgcaaaca cgtctcacgt tgatgagcaa tcgccaacag ggcggctcac
    12361 ctggcggtgt gtttgttatg ggggcgattg ccggactgat ctgttcacca tgcaccaccg
    12421 caccgcttag cgcgattctg ctgtatatcg cccaaagcgg gaacatgtgg ctgggcggcg
    12481 gcacgcttta tctctatgcg ttgggcatgg gcctgccgct gatgctaatt accgtctttg
    12541 gtaaccgctt gctgccgaaa agcggcccgt ggatggaaca agtcaaaacc gcgtttggtt
    12601 ttgtgatcct cgcactgccg gtcttcctgc tggagcgagt gattggtgat gtatggggat
    12661 tacgcttgtg gtcggcgctg ggtgtcgcat tctttggctg ggcctttatc accagcctac
    12721 aggctaaacg cggctggatg cgcgtggtgc aaataatcct gctggcagcg gcattggtta
    12781 gcgtgcgccc acttcaggat tgggcatttg gtgcgacgca taccgcgcaa actcagacgc
    12841 atctcaactt tacacaaatc aaaacggtag atgagttaaa tcaggcgctc gttgaagcca
    12901 aaggcaaacc ggtgatgtta gatctttatg ccgactggtg cgtcgcctgt aaagagtttg
    12961 agaaatacac cttcagcgac ccgcaggtgc aaaaagcgtt agcagacacg gtcttacttc
    13021 aggccaacgt cacggccaac gacgcacaag atgtggcgct gttaaagcat cttaatgtcc
    13081 ttggcctacc gacaattctc ttttttgacg gacaaggcca ggagcatcca caagcacgcg
    13141 tcacgggctt tatggatgct gaaaccttca gcgcacattt gcgcgatcgc caaccgtgaa
    13201 cgacacttgc agtgggaaag acggaggaaa atagcgtgca acgtgaagat gtactgggag
    13261 aagccctgaa attattagaa ttacaaggga ttgccaacac cacgctggag atggttgctg
    13321 aacgtgtgga ttatccactg gacgagctac gccgcttctg gccagacaaa gaggcaatcc
    13381 tctacgatgc gctgcgttat cttagccaac agatagatgt ctggcgtcgt cagctgatgc
    13441 tggacgaaac gcaaaccgcc gaacaaaagc tgctggcacg ttatcaggcg ttatcggagt
    13501 gcgttaaaaa caaccgctat ccgggctgtc tatttatcgc tgcctgtacg ttttatcccg
    13561 atcctggcca ccctattcat caactggccg atcagcaaaa aagcgcggcc tacgatttca
    13621 cccacgaact gttaaccacg ctggaagttg acgatccggc gatggtagca aagcagatgg
    13681 aactggtgct ggaaggctgt ttaagccgaa tgctggtgaa tcgtagccag gcggatgtcg
    13741 acactgcaca tcggctggcg gaagatatcc tgcgcttcgc ccgctgccgc cagggtggtg
    13801 cattgacctg acagaaacac agaaaagaag cgatttgccg caatcttaag cagttgaatc
    13861 gcttttactg aaattaggtt gacgagatgt gcagattacg gtttaatgcg ccccgttgcc
    13921 cggatagctc agtcggtaga gcaggggatt gaaaatcccc gtgtccttgg ttcgattccg
    13981 agtccgggca ccaaattcat atcaacggac ctccacggag gtccgttttt cgtttcagga
    14041 cgccacgatt taagcgttct gcctccaaat caattctacc gaactcaacc agattctccc
    14101 cacatcacca gcaatttgcg agcatatccc aatttgggaa aatttgttcc tgagctatag
    14161 cactaactga cgtgaaatgt cgtgcggccc cgtgatgctg ttgaacgtca aatgacgtca
    14221 tcaggagcgt aacgcatcca taaagcacaa caccggtctg aacgccgact gatgagattt
    14281 tctgaatgag aacaaagaga aatgtgtcag tccgcttgct catgcaaaga ctaaca
//
LOCUS       DADLSE010000097        13753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLSE010000097 DADLSE010000000
VERSION     DADLSE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.097"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(19..765)
                     /gene="dsbG"
                     /locus_tag="JN692_002865"
     CDS             complement(19..765)
                     /gene="dsbG"
                     /locus_tag="JN692_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7265137.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            1137..1700
                     /gene="ahpC"
                     /locus_tag="JN692_002866"
     CDS             1137..1700
                     /gene="ahpC"
                     /locus_tag="JN692_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7265138.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1945..3510
                     /gene="ahpF"
                     /locus_tag="JN692_002867"
     CDS             1945..3510
                     /gene="ahpF"
                     /locus_tag="JN692_002867"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7265139.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRASGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3631..4059)
                     /gene="uspG"
                     /locus_tag="JN692_002868"
     CDS             complement(3631..4059)
                     /gene="uspG"
                     /locus_tag="JN692_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7265140.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            4280..5518
                     /locus_tag="JN692_002869"
     CDS             4280..5518
                     /locus_tag="JN692_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HAY7265141.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5749..6159)
                     /gene="rnk"
                     /locus_tag="JN692_002870"
     CDS             complement(5749..6159)
                     /gene="rnk"
                     /locus_tag="JN692_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7265142.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6388..7194)
                     /gene="rna"
                     /locus_tag="JN692_002871"
     CDS             complement(6388..7194)
                     /gene="rna"
                     /locus_tag="JN692_002871"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7265143.1"
                     /translation="MKAFWRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            complement(7308..8771)
                     /gene="citT"
                     /locus_tag="JN692_002872"
     CDS             complement(7308..8771)
                     /gene="citT"
                     /locus_tag="JN692_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7265144.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIIGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8822..9700)
                     /gene="citG"
                     /locus_tag="JN692_002873"
     CDS             complement(8822..9700)
                     /gene="citG"
                     /locus_tag="JN692_002873"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7265145.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTIVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9675..10226)
                     /gene="citX"
                     /locus_tag="JN692_002874"
     CDS             complement(9675..10226)
                     /gene="citX"
                     /locus_tag="JN692_002874"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7265146.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRIEALLNDVDACNVN"
     gene            complement(10230..11761)
                     /gene="citF"
                     /locus_tag="JN692_002875"
                     /pseudo
     CDS             complement(10230..11761)
                     /gene="citF"
                     /locus_tag="JN692_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11772..12680)
                     /gene="citE"
                     /locus_tag="JN692_002876"
     CDS             complement(11772..12680)
                     /gene="citE"
                     /locus_tag="JN692_002876"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7265147.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12677..12973)
                     /gene="citD"
                     /locus_tag="JN692_002877"
     CDS             complement(12677..12973)
                     /gene="citD"
                     /locus_tag="JN692_002877"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7265148.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12988..>13753)
                     /gene="citC"
                     /locus_tag="JN692_002878"
     CDS             complement(12988..>13753)
                     /gene="citC"
                     /locus_tag="JN692_002878"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7265149.1"
                     /translation="TEYEALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRH
                     PGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGT
                     ADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRF
                     VGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKN
                     DLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3368 a         3496 c         3660 g         3229 t
ORIGIN      
        1 ccatccatga attacctttt atttatttcc cataatgata ttaagcgttt tctgatcggg
       61 caaccccacg gcctgttgca gcgtattttc cttactcatg taatagatag ccggcgtgac
      121 atttgccccc agatcgtcca tcagtttctc attgtcactt aacactttca tttgctctgt
      181 acttacgttt gcaggcacgt ttagcttaag cttgcaacca gaggcttcat attgttgcca
      241 ggtttttgcg ggatctttgg aggcaagaat tgccgctgct gtcgccgggc tttctggctt
      301 gataacccca accaacaatg ttcttaattg cactttgcca gaatctaccc acgggcgcgc
      361 ctgctgccag aactgtttac aatatgggca gaacggatcg gcgaagacgt agacaatgac
      421 cggcgcatct tttttaccgt cgaggagcca gtgggattgt tccatccgtt gccacatttc
      481 gcgtccggct ggtgcgtaaa tttctttttc gataagtgtg ttactcaggt tttcaccttt
      541 ctcgttgtac atgtaaccag agatagcgtg cttaccatcg ggagtcaggt agatggtgac
      601 gcccatatcc tgatactttc cgagataacc tttcattcct ccgggggcat cgaacgtttt
      661 gatgattgta atgccctgtt tttcaatcgc ttttactggg gaaggaagtt cctctgcgaa
      721 ggcgattgca ggaagcagag ccagtaaaag tatctttttt aacattaatt tgtccttttc
      781 agtcagtgca aaagtcgagt aaaaggcata acctatcact gtcataggta agagcttaga
      841 tcaggtgatt gccctttgtt tatgagggtg ttgtaatcca tgtcgttgtt gcatttgtaa
      901 gggcaacacc tcagcctgca ggcaggcgct gaagatacca aagggtagtt cagattacac
      961 ggtcacctgg aaagggggcc attttacttt ttatcgccgc tggcggtgca aagttcacaa
     1021 agttgtctta cgaaggttgt aaggtaaaac ttatcgattt gataatggaa acgcattagc
     1081 cgaatcggca aaaattggtt accttacatc tcatcgaaaa cacggaggaa gtatagatgt
     1141 ccttgattaa caccaaaatt aaacctttta aaaaccaggc attcaaaaac ggcgaattca
     1201 tcgaaatcac cgaaaaagat accgaaggcc gctggagcgt cttcttcttc tacccggctg
     1261 actttacttt cgtatgcccg accgaactgg gtgacgttgc tgaccactac gaagaactgc
     1321 agaaactggg cgtagacgta tacgcagtat ctaccgatac tcacttcacc cacaaagcat
     1381 ggcacagcag ctctgaaacc atcgctaaaa tcaaatatgc gatgatcggc gacccgactg
     1441 gcgccctgac ccgtaacttc gacaacatgc gtgaagatga aggtctggct gaccgtgcga
     1501 ccttcgttgt tgacccgcag ggtatcatcc aggcaatcga agttaccgct gaaggcattg
     1561 gccgtgacgc gtctgatctg ctgcgtaaaa tcaaagcagc tcagtacgta gcttctcacc
     1621 caggtgaagt ttgcccggct aaatggaaag aaggtgaagc aactctggct ccgtctctgg
     1681 acctggttgg taaaatctaa atttccttcg tctttcacgc catagcggcg ttggcgtcgc
     1741 ccgctcaccc cggtcactta cttgtgtaag ctcccgggga ttcacaggct agtcgccttg
     1801 ctctgacgcg aaatacttcg gaaattcacc taattcttcg ggtgctgcgg cacccgattt
     1861 tcttccccgc accatgatgc aagctgcatc caggtagccg cagaggccgc ttgcatgatg
     1921 atgtttaaag cccaggagat aaacatgctc gacacaaata tgaaaactca actcaaggct
     1981 taccttgaga aattgaccaa gcctgttgag ttaattgcca cgctggatga cagcgctaaa
     2041 tcggcagaaa tcaaggaact gttggctgaa atcgcagaac tgtcagacaa agtcaccttt
     2101 aaagaagata acagcttgcc agtgcgtaag ccgtctttcc tgatcaccaa cccaggttcc
     2161 aaccagggac cacgttttgc aggctccccg ctgggccacg agttcacctc gctggtactg
     2221 gcgttgctgt ggaccggtgg tcatccgtcg aaagaagcgc agtctctgct ggagcagatt
     2281 cgccatattg acggtgattt tgaattcgaa acctattact cgctctcttg ccataactgc
     2341 ccggacgtgg tgcaggcgct gaacctgatg agcgtattga acccgcgcat caagcacact
     2401 gcaattgacg gcggcacctt ccagaacgaa atcaccgatc gcaacgtgat gggcgttccg
     2461 gcagtgttcg taaacgggaa agagtttggt cagggccgca tgacgttgac tgaaatcgtt
     2521 gccaaaattg atactggcgc ggaaaaacgt gcggcagaag agctgaacaa gcgtgatgct
     2581 tatgacgtat taatcgttgg ttccggcccg gcgggtgcag cggcagcaat ttactccgca
     2641 cgtaaaggca tccgtaccgg tctgatgggc gaacgttttg gtggtcagat cctcgatacc
     2701 gttgatatcg aaaactacat ttctgtaccg aagactgaag ggcagaagtt ggcaggcgca
     2761 ctgaaagttc acgttgatga atacgacgtt gatgtgatcg acagccagag cgccagcaaa
     2821 ctgatcccgg cagcggttga aggtggtcta caccagattg aaacagcttc tggcgcggta
     2881 ctgaaagcac gcagcattat cgtggcgacg ggtgcaaaat ggcgcaacat gaacgttccg
     2941 ggcgaagatc agtatcgcac taaaggcgtg acctactgcc cgcactgcga cggcccgctg
     3001 tttaaaggca aacgcgtagc ggttatcggc ggcggtaact ccggcgtgga agcggcaatc
     3061 gacctggcgg gtatcgttga gcacgtaacg ctgctggaat ttgcgccaga aatgaaagcc
     3121 gaccaggttc tgcaggacaa actgcgcagc ctgaaaaacg tcgacattat tctgaatgcg
     3181 caaaccacgg aagtgaaagg cgacggcagc aaagtcgttg gtctggaata tcgagatcgt
     3241 gccagcggcg atattcacaa catcgaactg gccggtattt ttgtccagat tggtctgctg
     3301 ccgaacacca actggctgga aggcgcagtc gaacgtaacc gcatgggcga gattatcatt
     3361 gatgcgaaat gtgaaaccaa cgtcaaaggc gtgttcgcag cgggtgactg tacgacggtt
     3421 ccgtacaagc agatcatcat cgccactggc gaaggtgcca aagcctctct gagtgctttt
     3481 gactacctga ttcgcaccaa aactgcataa gaagaagtaa gattcacctg caattgctta
     3541 gccgccgggg tcaaacctgg cggctttttt atggcattaa aaagccctgc cgggatgttc
     3601 caggcagggc ggcataaata cttttatctg ttagcgaacg accagtacag gcagattggc
     3661 gtggcggatc acgctcgagg cgttagaacc taacagatgg gtcgaaatcg atgggttgcg
     3721 agaaccgata actacaacat cagcccccag ttcttctgcc aactcattga cttcatcccg
     3781 cacgctacca aaacggacat gttgtttaat gcgggaagga tcgatggtga agtggctgac
     3841 catcgtttgc agacgttctt ctgcttcatg ttgcagatgc tcttcaaaac gacgcacatc
     3901 agcggcaaaa cggtgcaggc tcaggctggc tgacccgggt agtacgtgaa gtagatgaat
     3961 aactccgtca tcctgggcga ggaattcagc gtggcgaaca gctttgtcgc tcaattccat
     4021 ttcaaataca tcaactggca taatgattgt cttatacata accctttctc cctgttaatc
     4081 atgaacaaat cattcgccat gattataata tttatccctg atatttgtct ggttcttttt
     4141 ccttacgaac ggtttctgtg attaatatat tctcactgaa caccaggaat tctcccaaaa
     4201 cctgcggtac cgcccgtttt cccgctgtga tagctaccct taaagactga ctcttttttg
     4261 aactgtctct ggaggttgca tgaaagcatt gacttatcac ggcccacatc acgttcaggt
     4321 agaaaatgtt cccgatccgg gcattgaaca ggcagatgat attattctgc gtattacggt
     4381 aacggcgatc tgtggctctg acctccatct ttatcgaggc aaaatacccc aggttaaaca
     4441 tggcgatatt tttggtcatg aatttatggg ggaagtcgtt gaaaccggga aggacgtaaa
     4501 aaatttgcaa aaaggcgacc gggtggtaat tccgttcgtc attgcttgtg gcgactgttt
     4561 tttctgtcga ttacagcaat atgccgcctg cgaaaatacc aatgcgggta aaggcgctgc
     4621 gctcaataaa aaacagatac ctgctcccgc ggcattgttt ggttatagtc acctgtatgg
     4681 tggcgttcct ggcgggcagg cggaatatgt ccgcgtccct aaagggaatg tggggccgtt
     4741 taaagtaccg cctttgcttt cagatgataa agcccttttc ctttctgata ttctgccaac
     4801 ggcatggcag gcagcaaaaa atgtgcagat ccaacaaggt tcaagcgttg cagtctatgg
     4861 ggctggtcct gtgggattgt tgacaatcgc ctgtgcacgg ttgctcggtg cggaacagat
     4921 ttttgttgtt gatcatcatc cctaccgctt gcgtttcgcc gctgaccgct acggcgcgat
     4981 cccgattaat ttcgatgaag atagtgatcc ggcacagtca attattgaac aaacggcagg
     5041 tcaccggggc gtggatgcag taatagacgc cgtcggtttt gaagcgaaag gcagcaccac
     5101 ggaaacggtg ctgactaacc tgaaactgga gggcagcagc ggtaaagcgt tgcgccagtg
     5161 tattgcggcg gtcaggcgtg gcggcattgt tagcgtaccg ggcgtctacg ctggatttat
     5221 tcacggtttc ctgtttggcg acgcctttga taaagggttg acgtttaaaa tgggacagac
     5281 ccacgttcac gcatggctgg gagaattatt accgttaatt gagaaaggat tactgaaacc
     5341 agaagaaatt gttacccact atatgccgtt tgaagaggcc gcccggggat atgagatttt
     5401 cgaaaaacgt gaagaggagt gccgtaaggt gattctggta cccggtgcac aaagcgcaga
     5461 ggcggcgcag aaggcggttt caggtctggt gaatgcgatg ccggggggaa caatatgatc
     5521 gtcaggagtg gttttcgagg taaaggacag ccatgacaat aatcgccgcc ataatcagaa
     5581 atcctatcag gatgtaaaat gcttctgcca tggttattcc cacaaacgaa acggaataat
     5641 tttgcagcaa aagtgaacag tgagaaccag gaaaaattgc tgattttgcg taaagaggat
     5701 gcgagtgcat cctctgggca aagcgagtta tcgcttgtgc agatgggatt aaagcaggta
     5761 gtcgccagca gcttctggct ggtattcgag ttccagcact tcaaggtggg ttgcaacgcc
     5821 gcccggaagt tcccagtgaa tggaatcgcc aacgcgcagc cccagcagtg cggcacctac
     5881 cggagccata acggaaagct gagtattgct atcggtcatt tttgccggat acaccagcgt
     5941 gcgcacacgc acttcgccat cgctaagatt gcggaattta acccggctgt tcattgtcac
     6001 cacgtcgtgt ggcatctctt ctggcgaaca catttgggcg cgatccaact ctgcgtttaa
     6061 cgcgtcggcg attggcaaac cagcataggc gggttgctcc agcagaatat cgatgcgttc
     6121 ggcatccagg tcgttaatga tgatagttgg tctggacatt tttactccat gtcgtcggta
     6181 ctgcgagtgt cgcagataaa catacccaaa agaaaaccct caccgtcagg cggcgagggt
     6241 ttaactcaca tgatgatact gactgttgct cactctttga agtgatttgc gtcacattca
     6301 gggaattcct caatgcagcg cattatgtat aattcttaat cggcatggtt atggaagacg
     6361 aattagcgtg ttttgtaaat cagaggatta ataacccgct ttatcaatca caaaggtttt
     6421 gccacagtta cctgggtgag gttgtggcaa gaatgagttt gcagaaagcg gagcgttgat
     6481 ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac cctggcacgt
     6541 tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg cgtcaaagtc
     6601 acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt cgctctcttt
     6661 aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa aaccaaagca
     6721 ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag ctcctggcat
     6781 gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg atgaacacat
     6841 tcggctggcg cgtgcttctg gtagattcgg gattgggcga gtagcgcaac cgaagcgcat
     6901 ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg gccacagacc
     6961 atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgcaggcgac attcatctcg
     7021 ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg agagggccag
     7081 gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt tggcagaaga
     7141 taagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt tcataatggg
     7201 tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc ccgccaggtg
     7261 ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta gttccacatg
     7321 gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc gaagattgcg
     7381 ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat caccccagga
     7441 ccggttgcat acggcgtcag acagcctatg ataccgatag acagcaccag caggatacac
     7501 agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac cggcagcatg
     7561 gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa caccagaacc
     7621 agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat ggtaccggcg
     7681 aaccagtcaa taaaaccaga acgagtcagg ccgttagcca tcacaaccag agttgccagg
     7741 ttgaccagcg tgttccatgc gctgttatag cgggtaatgt ctttccacgg cacaacgtgc
     7801 agagccagca ttagcgaaac tgccagcaga ccaaccgcag tagcattaat gacttcactg
     7861 ccaaataccc acaaacctaa gctgagcaat acaaggccaa tcagcgtcca ctctctgcgt
     7921 gtcagcgcac ccatggtttt tagttcatca cccgcccagg ttgccacttc ttcactgtgt
     7981 gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag caagataacc
     8041 ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc ggcaattttg
     8101 ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat ggacgaactc
     8161 agactggtac taatgaccat catccacatc aaatagccgc caatacgacg cgcggacgga
     8221 tcgttcggga atgatttaaa caacggcggc aggtttttaa tgaccggaaa aaccgtaccc
     8281 ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat aatgacaatc
     8341 gcataaccca acgtcagcgt gcgtttgccc atgaatttca ctaggaaaag ggcaatgcga
     8401 cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa taccagccat
     8461 accgtggtgc tggaaaaacc agccaggccc catttcagcg cctgtttttg cgcattaaac
     8521 gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat aacgcaaata
     8581 gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat gccgacaatc
     8641 attgccacaa acacagcgaa gtaatgccat gcctgcggcg gcataccgtc ggggacaggg
     8701 ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat attatctttt
     8761 gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag tgcttaaata
     8821 attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg cctggactga
     8881 gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg gcgggggttc
     8941 gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc aggcccccct
     9001 cgccaccgcg cgatgcaacg ttggtatcgc cgttggtagc catcagtagg agcaaggtat
     9061 cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa tgcggcaagg
     9121 cgtggttgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg gtaaggccaa
     9181 gctgctggta caaccgttga cctgccgtca gtcgtgaatt attggtacgc agttcgcgat
     9241 cggtcaggcc acggcagaaa cttgccgccg tagaacaaac gattgttggc gtaaccggct
     9301 ggttgagctg aagcaaacgg ccaattgccg cacatagcag ccctaaagaa aaaatgctgc
     9361 ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa gccataccaa
     9421 ttgggcgtaa tccgtggagt accgcttccg gtgccatttc tgcactacag gcaccaaatt
     9481 caatgaaacg gggtaaccag ccctgaatcg ccagcgcgct gcggtggaaa tcttccagcg
     9541 ccatatcttt gtgcgcaccg cagttaatgc gatccacgag gcctggtttc ggtgacagat
     9601 tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatt aatgacgtcg
     9661 caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag tgcctctatg
     9721 cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc tgcgctttgc
     9781 tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag aatttcgcct
     9841 tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg ttcaagctca
     9901 atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc ctccggcccg
     9961 ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgctttgt ggctaaggca
    10021 cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc tttaatcggc
    10081 ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggtgcttgag ccaggcgtgt
    10141 tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac cgcatggtgg
    10201 ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac aacatcgatc
    10261 accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc aatcggctgt
    10321 ggttcaccgg tcagcagacg cgcacgttcg cgcagccact caatggaaac cactttaatg
    10381 cccgcttcct gcagacgttc tgctagttcc ggacgtgccg ggttaactgc gataccgtgg
    10441 tctgtgacca gaatatcgac actggagccc ggggtgatgc aggtcagcac gttatccacc
    10501 agagtcggaa tacgaccgcg taccagtggc gcgacgatga tggaaagcgc agaggcaatc
    10561 gcggtatcgc agtgaccacc ggaagcgcca cgcagtacgc cgtcagaacc ggtcagcacg
    10621 ttaacgttga actgcctgtc aatttccagc gcgctcagta ccaccacgtc gagacgatca
    10681 accgatgcgc ctttcgaccc cagttagcgt actggttggc gctgatttcg atgtgattgg
    10741 ggttacgggc cagcgattgc gcagcatggc tgtcaaagct ctgcacatcc agcagtttgc
    10801 ggatcagacc tttttcgtgc agatcaacca tcgtcgcggt aataccgccg agggcgaagt
    10861 cggcgcgaat atcgcggcta cgcattttgt cttccaggaa acgggttacc gccagcgaag
    10921 cgccgccggt gccggtttgc atggagaaac cttctttgaa gtagccagag ttgacaatca
    10981 catccgcagc gctacgggca ataagcagtt cgcgcgggtt agtggtcata cgggtagtac
    11041 cagcgccgat ttttgcagca tcgccaacgc gatcaacttt gacgatcaaa tcaacctgat
    11101 cttgctcaat gcttgccgga ttatgcggat aaggcagcag ttcttcggta agcatcacga
    11161 cctgttttgc gttgtcggca tcaaccattg catagccgag ggagccgcag caggctttac
    11221 cggtgtagcc gttggcatta ccgaattcat cacaggacgg gacgccgagg aaagccacgt
    11281 cgatattcag ttcgccgctc tgtaccagat gcacacgacc gccgtgagag tggatctgca
    11341 ccggttccgc cagcagacca cgggagatct cttccgccag tggaccgcgt aggccagagg
    11401 tataaatgcg ggtaaccacg ccctggcgaa tgtgttctac cagcggcaca tggcaatcac
    11461 tcagggagct ggacgccagg gtcaggtttt taaagcccat cttcgcgatg acgtccatca
    11521 ccatattgac ggtcaggtca ccgccacgga aagcgtgatg gaaggaaacc gtcatgccgt
    11581 cctgtaaacc tgagcgacga atcgcttctt ccaggttggc gcacagtttg cgatcgcgcg
    11641 ctttttcagc ctggtaggtt tgctttggtg agttctggaa agcggcaaga tcgcattcag
    11701 tgcgacgatt ccaggccgct acccgttctt gtcgttgaga ttgttcaatt ttctgcgtca
    11761 ttttgattgc cttattcttc gcggatgccg gaaagttctg cacgggagag caccagacgg
    11821 gcgcgatcga taaccggacc gtccaccatc ttgccgttca gggaaaccac gccgaggcct
    11881 tcgcgagcgg cggcttcagc ggcttctacg acgcggcggg cgtgatccac ttctttctgg
    11941 gtcggtgcgt agaggttgtg cagcagatca atctgacgcg ggttgatcag cgatttgccg
    12001 tcaaagccca gctgtttgat gtgggcggct tcttgcagga atccggcttc gttgttagcg
    12061 tcggaataga cggtatcgaa cgcctgaata cccgcagagc gcgcggcctg caaaatggaa
    12121 cagcgtgcga acagcagttc agttccttcc ggggagcgtt ctgtacgcag gttgcgcaca
    12181 tagtcttctg caccgagggc gataccgatc aaacgctcgg aagcgtgagc gatttccact
    12241 gcgcgggtaa tgcccagcgg agattcaatc gccgccagca ggccggtgct gccgggttca
    12301 cgaccacagg ctttttctat acgcaggatc tctttttcaa tatccagaac atcctgagcg
    12361 gtatcggttt tcggcagacg cacaacgtcc gcaccaccgc gaacgacggc ttccaggtcg
    12421 ttaacacccc attcggaatc cagcgcgttg acacgcacaa tggtttcaat atcgcgatac
    12481 agcggatgtt gcagcgcgtg ataaaccatg cggcgggcgg tgtctttttc acgtaatgcc
    12541 acggagtctt cgaggtcaaa catcagggca tcagccgggt agatgaagga gttgctgacc
    12601 atcgcggcat tggcaccagg cacaaacaac atgctgcggc gggtgcgagt tttacgttgt
    12661 tgcagcgaag cggaaatcat tggcaatcct cccatggcag agccgggata ccgctggcgc
    12721 gtgccagcag ggcttccagt cgtgcacgta aaatgcagtc cagtgcgcct ttgtcatcga
    12781 cattcagctg tacgccgcgc acgttgtagc gggcgagaac gtccagaatg gtggtgcgaa
    12841 ttgcatcgcc aaactgtttc tcaacgctgc tattgatttg caggtcgata tcctgcgtat
    12901 cgagtggggc gatgcgtatc atcacatccc cagactcaag ggtgcctgca acggcgggct
    12961 ggtttatttt catttttcac ctgtttctca tgcgggggtc ttttgacgag ctgccgcgtc
    13021 ctggcgggag tgctcaagca ggttctgcaa ataatgcagc gtgactgcag ggaccagcgg
    13081 cgcgatagcc gtgagatcgt ttttcgccag cagttgacgt acccgggaag cggatatcgg
    13141 catctcctgg taacgcagcc gctcaatttc aaccagttcg atgggcgctg cggagatagt
    13201 cggcgtttcc agccagtagc gcatatcctg gttgtactgg gcggtaacgc gacaaaaggg
    13261 ttcagtaccg acaaagcggt gagttacacc aagcgcggga gcgaggtact gacggaaaat
    13321 cttcagatca atttcggtgt aacaatggtt aatgacgctc tgttctttaa tgaagtagca
    13381 agggaacgta gcgcgggaga tgatgtattc tgagccgcga tgcacagtca ggcgtggaat
    13441 atcggcggtg ccttttaaca ccagatccag ccggtcttca taggggaagc gtgaagaatc
    13501 ttctttgact aaaaacagat gcaaccagtc gcactgtgcc gcagcttgtt gaatcagata
    13561 acggtgacca ttcgtaaagg gattggcgtt catcacaatg cagccaatct tgttccctgg
    13621 atgacgaaat tttttcagcg attcggcata gcgtttcagt cgcgtggcgc tgttttccat
    13681 cagcaccatc acgccgggta cgcaggtcag cgtggaaaaa ccgcactggc ggaacagcgc
    13741 ctcgtattcg gtt
//
LOCUS       DADLSE010000098        13585 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLSE010000098 DADLSE010000000
VERSION     DADLSE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.098"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..896)
                     /locus_tag="JN692_002879"
     CDS             complement(<1..896)
                     /locus_tag="JN692_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7265150.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="JN692_002880"
     CDS             1137..1937
                     /locus_tag="JN692_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7265151.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="JN692_002881"
     CDS             2015..2785
                     /locus_tag="JN692_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7265152.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYFAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN692_002882"
     CDS             complement(2833..4191)
                     /gene="mltD"
                     /locus_tag="JN692_002882"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7265153.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN692_002883"
     CDS             complement(4263..5018)
                     /gene="gloB"
                     /locus_tag="JN692_002883"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7265154.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5052..5774
                     /locus_tag="JN692_002884"
     CDS             5052..5774
                     /locus_tag="JN692_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7265155.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN692_002885"
     CDS             complement(5771..6238)
                     /gene="rnhA"
                     /locus_tag="JN692_002885"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7265156.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWLHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN692_002886"
     CDS             6303..7034
                     /gene="dnaQ"
                     /locus_tag="JN692_002886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7265157.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7167..7243
                     /locus_tag="JN692_002887"
     tRNA            7167..7243
                     /locus_tag="JN692_002887"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7201..7203,aa:Asp,seq:gtc)
     gene            complement(7374..8420)
                     /locus_tag="JN692_002888"
     CDS             complement(7374..8420)
                     /locus_tag="JN692_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7265158.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFTRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8431..9346)
                     /gene="tssG"
                     /locus_tag="JN692_002889"
                     /pseudo
     CDS             complement(8431..9346)
                     /gene="tssG"
                     /locus_tag="JN692_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9463..11346)
                     /gene="tssF"
                     /locus_tag="JN692_002890"
     CDS             complement(9463..11346)
                     /gene="tssF"
                     /locus_tag="JN692_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7265159.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11362..11856)
                     /gene="tssE"
                     /locus_tag="JN692_002891"
     CDS             complement(11362..11856)
                     /gene="tssE"
                     /locus_tag="JN692_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7265160.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11853..12683)
                     /locus_tag="JN692_002892"
     CDS             complement(11853..12683)
                     /locus_tag="JN692_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7265161.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12670..13584)
                     /gene="tagK"
                     /locus_tag="JN692_002893"
                     /pseudo
     CDS             complement(12670..13584)
                     /gene="tagK"
                     /locus_tag="JN692_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3106 a         3544 c         3628 g         3307 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaaaagat gagatatttt gaatttattt gtcattaaac catcaagatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac cggagtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaagcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcggc tggcggtgcg tctggctgac tatcctggtg caagtgtact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattt tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcgctact gcctgcacca atcgtcaaac tttggcctgg
     3061 cttcagctta gatccgcgca gtttgttcca ctgctgcaaa tctttggtgc ttacgccgag
     3121 acgtgaagcg atgcttgaaa gcgtgtcgcc agagcgtacg gtgtaaacac ggctgttaag
     3181 cggcgtattg tcggcaacca gcgtcgactg tacagcagca atttcgcctg aagcgagaga
     3241 ttcacgcagt tgatctgcat gcttctttgg caccatcacg tactgcggcc cacttgcgcc
     3301 cagcgtggag cctttcacgc cagcgttgaa tgtcttcagc ttgctgacgg aaatccccgc
     3361 catatctgca accttcgcca tttcaaccgg gctactcagg tgcacacgcg ccagagcacg
     3421 gctttcatcg gtcgttggca gacgtacgcc ataacgcttg ctgtttttga gaatatcact
     3481 caatgccagc attttaggca cgtactgctt cgtttcctgc ggcaacggta acgaccagaa
     3541 gtccgtggat ttcccacgcg ctttgttcgt tttaattgcc ttcatgaccc gaccttcgcc
     3601 gctgttataa gccgctacgg tcagaagcca gtcgccgtca aacattttgt tcagacgctg
     3661 catcatattc agcgcggcag ttgttgaagc aacaacatcg cgacgcgcgt cataattgcg
     3721 ggtctgtttc aaaccataat tgcgccccgt gctcggaatg atctgccaga tgcctgcggc
     3781 attggcgcca gacgttgcgt gaggatcaaa agcgctctcc actatgggta gtagtaccag
     3841 ttccataggc atgttacgtt ttttaacttg ccctgctatc cagtacatat acggctctgc
     3901 ccgtaaagtt acatcgtgga gatagctctt attgcgtaaa tatttctgtt tctgttcgcg
     3961 aatccggtca ttttccggaa ttcccatctt tagctcgtcg ccaatgaaag cccacaagtc
     4021 accatctggc gcgatagacg tcccatcgtc catccatcgt gcctgacttg taaactttgc
     4081 tgcttcccct tgaccagctg cagaaaggct ctgtgcgtgc tgttgaacgt tgccggtact
     4141 ctggcaaccc acgagcagga cagaggcgag taatatcgct tttgccttca tgtgtgtgtc
     4201 aatagttgct taaaagacga ccgatcataa cggcgaacgg agccgatgac aagaaagttt
     4261 tatcagaacc tatctttctt tgaccttaac catgcaaaac gctcttcagg ttgttgcaat
     4321 aatgtttctt cattaattac attaattaaa tcaatatctt ccgttcttaa aaaaacatta
     4381 atttgccgct catttttcag aattacgggt agtgttattt gattttttgc ccgtaactcc
     4441 ttaactttac gataataatc atttatggac aaatcgtgcg gaaaaatact caaagcaaac
     4501 ttcatatttg ataacgtata ttcatgagca caacatacca atgtatcgtc aggtaacgca
     4561 cttagctttt taagtgattg atacatttgt aaagctgtcc cttcaaacaa ccgaccacac
     4621 ccaccagaaa acagcgtgtc gccgcaaaat agataaggtt tactgaagta acagatatgt
     4681 cctaaagtgt gacccggtgt ggtaattaca ctaaattcat gccctaaaac gaaggcagtt
     4741 tcgccatctt tgactacctg tgttgttccc ttatcttgtg tctcttgtgg accatacacc
     4801 acaatttgcg gaaacttttc caccagttct tttacgccgc caacgtgatc gtggtgatgg
     4861 tgggttagaa atatggcctc cggttgccag ttattggcgg taatggcgtt taatactggc
     4921 tctgcgtctc cgggatcgac aatcaggcag cgacctgcct catcattcaa aacccagatg
     4981 taattgtcat caaaggcggg aatactgtta agattcatag attacctctc agtgtaaaac
     5041 ggaaggttgt gatgaaaccg gcaagagtcc ctcaaactgt cgtggctcct gattgctggg
     5101 gcgatttgcc ctggggagag ctttatcgca aggcgctgga gcgccagctc aacccgtggt
     5161 tcactaaaat gtatggtttt catctgctta agattggcaa tttaagcgca gaaatcaatt
     5221 gcgaagcgtg cgcggtttct catcaagtga atgtttctgc gcaaggaatg cccgtccagg
     5281 tgcaggcgga tccacttcat cttccttttg ccgataaatc cgttgatgtt tgtctactgg
     5341 cacatacctt gccgtggtgc accgatccgc atcgtttatt acgtgaagcc gatcgggtac
     5401 tgattgatga tggctggctg gtgattagtg gcttcaaccc catcagttta atgggattac
     5461 gcaaacttgt gccggtattg cgcaaaacct cgccctataa tagccggatg tttactctga
     5521 tgcggcaact ggactggctc tctttgttga attttgaagt gctgcacgcc agccgtttcc
     5581 acgttctccc gtggaacaaa cacggaggaa aactattgaa tgcgcatatt cctgcgcttg
     5641 gttgcttaca acttattgtt gcccggaaac ggactattcc tttaacgcta aatccgatga
     5701 aacagagtaa aaacaagcca cgaattcgcc aggcggttgg agccacccgg caatgtcgta
     5761 aaccacaggc ttaaacttcg acttggtagc ccgtatcttc cagtgtggga ttcatcgccg
     5821 cggcacgagc cagttcatca cagcgttcgt tttccgggtg tccggcatgg cctttaaccc
     5881 attcccattt gatttgatgc tgccccaatg cagcatcaag acgttgccag agatcgacat
     5941 tttttactgg ttttttgtct gctgttttcc agccacgttt tttccagtta tggagccact
     6001 gggtgatacc ctggcggaca tactggctgt cggtactcaa aatgacttcg caatgttctt
     6061 ttaacgcctc cagcgcgaca atagcggcca tcaactccat acggttgttg gtggtgcggg
     6121 tgtagcctgc gctaaaggtt ttctcgcgtc cgcgatagcg taaaatagcg ccgtaacccc
     6181 caggtccagg attacccaga cacgaaccat cggtgaaaat ttctacctgt ttaagcatct
     6241 ctggtagact tcctgtaatt gaatcgaact gtaaaacgac aagtctgaca taaatgaccg
     6301 ctatgagcac tgcaattaca cgccagatcg ttctcgatac cgaaaccacc ggtatgaacc
     6361 agattggtgc acactatgaa ggccacaaga tcattgagat tggtgctgtt gaagtagtga
     6421 accgtcgcct gacgggcaat aacttccatg tttatctcaa acccgatcgg ctggtggatc
     6481 cggaagcctt tggcgtacat ggtattgctg atgaattttt gctcgataag cccacgtttg
     6541 ccgaagtagc cgatgagttt atggactata tccgcggcgc ggagttggtg atccataacg
     6601 cagcgttcga tataggcttt atggactacg agttttcgtt gcttaagcgc gatattccga
     6661 agaccaatac cttctgtaag gtcaccgata gccttgcggt ggcgaggaaa atgtttcccg
     6721 gtaaacgcaa cagcctcgat gcgctatgtg cccgctacga aatagataac agtaaacgaa
     6781 cgctgcacgg ggcattactc gatgcccaga tccttgcgga agtttatctg gcgatgaccg
     6841 gtggtcaaac gtcgatggct tttgcgatgg aaggagagac acagcagcaa caaggtgaag
     6901 caacaattca gcgcattgta cgtcaggcaa gtaagttacg cgttgttttt gcgacagatg
     6961 aagagctggc agcacatgaa gcccgtctcg atctggtgca gaagaaaggc ggaagctgcc
     7021 tctggcgggc ataaatacat gtaaaaggcg ctaaaaatag cgacttgggc gatttttgca
     7081 gcaaacgatt caaaagatga gaaaaatcgt tgacgaaggt cgaggcaatc cgtaatattc
     7141 gcctcgttcc caacggaaca caacgcggag cggtagttca gtcggttaga atacctgcct
     7201 gtcacgcagg gggtcgcggg ttcgagtccc gtccgttccg ccaatattca gaaagcccga
     7261 atcagtaatg attcgggctt tcgtcgtttt ggtgaagtga gaatttttca ctccgaagta
     7321 aaacggatat gatttaaaaa atggccagac attataaatt ctggccactt ctgttactcc
     7381 agcgcatccc atttctccag cagttctact ggaatttcat ttattacctc cgagacgcgt
     7441 tttccgacca ggcgttctgc ctggcgcagc aaaggaatgg tcgggctgct cggttcgctg
     7501 tgctcaaacc agcgacggat gacacgcagg cgttccagcg cgtcgtttcg gtcgcggatc
     7561 tgccggggtt ctgcgccggt catcggtgat atttcctccg cgatatgcac cggtagcgca
     7621 atctgctctg cctgcatctg ttcagtatga acgatggtgg cgttttccgg ctgctcagcc
     7681 tgtggttgtg gcaggatctc cggcgtggtg ctctgcactg ctcccggtaa cagggccagc
     7741 agttgcgtca gacgggtgaa gtccggtgcc tggtcattca gcgtttcacg cgcatggcgt
     7801 tgcagccgct cagtggtctc tgctgcctgg cgaaaggcat ccagcggcag tgtgccgcgc
     7861 gcttccaggt ctgccagttg ctggcgtacc gattctggtg ccagcgcatc cgccgggcgt
     7921 aatgcagata gcgaacgctc cacatcccgc acctgaaggc gcatggcagc attatttgac
     7981 agtgtaatgc cacgaatatc cgccattaca ccttcgtggt ccagcaacgc cgccagcgcg
     8041 ttactgcgtg ccaccgcagc atcttcggcg gtgatatctt cagtcgcgag cagttgagga
     8101 tggatggcgt ccgggtacgt ggagcacaac gtttccagca gcgtgagcgc ctccgacagt
     8161 cccctggcac cggcctgctg gatacggcag cgcagcagga caaccagaat gcggatatct
     8221 ttgctgcgca tcagcagacg atgggcgtcc cgctcaagct ccgcccagtt aatgttttcc
     8281 ggcgtactga caaaatcgcc atattgcgct tcttcacgcg gtgcggcgcg ggtgaacagc
     8341 agcaggtatt ccggatcgta ctccagatcc gggccgcagg ggcattccgc cgaaattggg
     8401 tcagacagga atatttccat ttaattatcc tcagtgcgcc aggtagtgtt ctggttcgaa
     8461 aatcatgccc gtcaccgggt tgtcgtgcat ggctttaccc gcccatgccc gtcaccgggt
     8521 tgtcgtgcat ggctttaccc gcccatgcac gacaacccag ttggtacgga ccaccggtaa
     8581 cagccggagg ggcggcgtgg ggctttagct gtagctcaat ttcccagcag tattcataac
     8641 cactaaaggc gcggaccagt tctgtcagga cgggcaggtt attgctgcca ggcagaaaac
     8701 gtcggtaatc ctccagcgtc agcgggccga taaccagccg gaacttatac tgcatgtccg
     8761 gcacggcctg gccgatgagt gcaccattgc caataacgga agagggggca ggcatcccca
     8821 gccgggtgat ctcatcgtcc gccacggcta tccagtgcag gacatactcc tgtatccgga
     8881 aaggtacacc gaaatagtgc gccagcgtgg tggccagacc gtccggattg cgggactcac
     8941 gcaccagatg ggccgatgcc gccagacgag cgtgatctgg caacgggctg tcagcgctct
     9001 cccgcaaatc ctgcccgctg aggctggcga cataaaaggc aaagcggtca tgttgcggtt
     9061 tatccagacc gcccccagcg gactgggcgc tgcgccatgc ctggtaaaac tgcgtcagcc
     9121 agcggtgatg aaaaagatca gaaaaacggg tcagcgtcgg atcgcgtcgg ctttcagtcc
     9181 ggttacgcgc cagttcggtg tagtgcagcg gcagtggacc gttcggcccc cacagcccga
     9241 ggctgtacag actgatgtgc aggcgccctt cctgccagcc cacactggcg atttctcgtg
     9301 gcgcaaaggt cagcgcgggc gtttgtccga ggcgaaattt ttccatccgt ggttgctagg
     9361 tgttgcccgc cggaacggta cacaactgca catccacgcg gcgcatcagg ttcagaaaac
     9421 cgtaacgcca ggggggttcc tgcgcctgag tgaaatcgtt gctcatacgc ttccccgctg
     9481 tcccgccctg acaggccagg tcataatttt gccgcgctgc atgctgtgca gcgtcatctg
     9541 cgagaaggta ttaatggaaa catggcgggc gatatagtgt tccagcacca ggccgaacag
     9601 atacggactg atgccggaaa aaccttcttc atccaccgtc agttcacaac tgacgccacg
     9661 cccatagacc agtagtccgg aaccgggcag acggcgggtg accggggtcg ttttacaacc
     9721 aatcaggctg cgcacctggc gcgattgcgg actgtcatga gcaggaataa acaggttcag
     9781 cagatctcgc aacgcctggc cgccggtacg gtgatccaga tcggccagcg gcagataatt
     9841 aaaagataac tggcggatca accgccatgc catctcacgt tcagccagcg ggggctgcgg
     9901 cgagcgtggc gggcggataa gccctacgcc cgccaccggg atggctgcat ccacggttag
     9961 atcgtcccgg ccattgcggg caataagaca gggcagatcg cggttagtga ccatggccgt
    10021 gatggtgatg tggcgcagat tttccgggta cggcgcttca tactggtcaa ccagcgacag
    10081 gaacacttcc gagcccgtat agggggtacg ggtgccgtaa cggcgggcgt tttctgataa
    10141 gcggcggggc tcccggcgca gggaaaaata acgtccgtga ttgccttcat cattattgcg
    10201 ggtgtggtag agcgggcgaa aggccatctt ccgtgtggtg tcggtttcca gcccttccac
    10261 ttcttggacc gagaacacct cataatccag cggatgggtt ctgtccacca ccagatgctg
    10321 ttccgtcgtg ctgtgcgtga catcgatccg ggcggtgacg cggggaaaca ggttgataac
    10381 cggggtacag aacaggctga actgtgccgc atttgtctgg tggatcagcc agtccggcgg
    10441 caggcggttc agcaggataa caatctccgc cacgccaccg tcaattttct gtaatccggc
    10501 agacaggcct gtcggggtga aaaaatagaa gcgttccgga caggcaaaat attcatgcag
    10561 caggttatga ccgtgaaaca cgttccacgc cagcggcagc agcccctgat ccggctccag
    10621 gccttcgtac ataacgggct gttgcagatt cacatccagc gcgccgtcaa aatgccccgg
    10681 aatgccagcc agggtcgcga tggcgctggt atgtagaagt tctaacaggt gtgaggccgt
    10741 acgctcctcg ccgcagagat aaaacggcag cctgtccagt ccggcaagct gactgaacgt
    10801 tagttcacca aaggtgcgca gggtaatacg cagcgccccg gcgacgtgaa tgttggccgg
    10861 caggtagcgg tgcagggcag gcatatccgg tggcgcggcg gtcagtcgcg cctcttcgat
    10921 ggcgagcggc cacagcgtta catcctggct gctgcgaaac tggcaggcag tgctttcgcc
    10981 ttccggaatg gcggagaaaa aggctgtgtc gcgcgggacg gtaaatcctt tcgccagatc
    11041 gccttcttcc gtatcgggat gcaactgcgc cacgctcatt gagggcgtgg gtgtgacgta
    11101 attggggctg accacttcca gcaggcgctg ggtgaaacgg gggaattcag cgtcaatttt
    11161 tagctgcgtg cgggcggtca ggaagctgaa ggcctcgacc attcgctcta catacggatc
    11221 ggcaatatcc gttccctgca tgcccagccg ggcggcgact ttgggatgac gggcggcaaa
    11281 ttcggctccc gtttcccgca gatagctcag ttcgcggttg tagtattcca gtagccgtgg
    11341 atccatcggt ctctctctga attaaaaaaa cgtaatgcga ctctgctcca tatccagcgc
    11401 gctacgtacc cggaatgcag tcggatacgg ctgcgtgagg atctcgccgc gaatttcaaa
    11461 ctgtaatgtg ttatggccac tctggcggcg ctcatcctga agtgggatga tttgcagggt
    11521 ggacgctttc agccggggtt caaaccgggt gatggttcgc aaaatggccc tgcggatatc
    11581 gtcccacttg tgctcataca gaaagctgcc cgccagtggc ggcaggccgt agttaagtac
    11641 ggacgctgcc gcgtacggat aacgtgcagc atcaatatca tcttcatggc tgacggtgtt
    11701 gagcagaaat gacaaatccc ggcggatgat ctccttaatc tgaaccgggc tgacgctgat
    11761 accgtggtcg cgtttctggt ttggggcgtc gtcacacagg cggtcaaaca gcgtgggcag
    11821 caggtgattg cctggacgct ggcgggaagc gctcacgcgt cgcttccttc agcggtgtta
    11881 aaggtacagg tggacaaatc cagcaggctg atatcgccgt gaccggtgag ccacaccctc
    11941 tgacccagcg cctgtaccag tgtctctccc ggaccgtcct gccagaccgt ttcccggcac
    12001 agacgcaggg agtcggcagc cttttcagag ccgctgtagc ggggaaacag ccaggcactg
    12061 tgtgtatcgc cattgttcag ggtgacgttg acgggtttcc agatcaaatc gatcagatgc
    12121 gcaggccgcg gcgatttcag cgaacagatt tgtgaaaacg gcagccagat atatgctccg
    12181 cccgcgatca gttcgagtac cggaccggta cgggagtcgc tgtcgctaat ccagtcaaat
    12241 gcgccgccat tccactgccc ggaagtgtct gttatggcct caagcgcgcg gctgcggtga
    12301 gcatcagctt cctgcgcttc accacgggcg ttacaggcga gggcggtcag cagatcctcc
    12361 atccaggcgg gcggcgggag aataacaccg ggccgctgtt cgccctgaaa gcaggcgtgg
    12421 cggtatatct cacagcggat cagctcgcca aatacctgcg cttcgcgagt ataatttgcg
    12481 tccatccggg cgcatagctg gatctgttgc agtgcccgcg accagtcgcc tgccacgcac
    12541 aataactgaa acaggctgtg gcggcgggag gcctgcgctg ggtgcatcct gacctgttgc
    12601 tctgccatct gaatgccctc caccacggaa taatcccgca gcagcgccga caaggtaatc
    12661 ggtagctcgt tattgtttgt catgatatat ctctccgtgt tgtgccgggt ttatccggta
    12721 atggctgtca atgccaatga tgcgatgctc tttccgtgtc agatctggca gtacggtgtt
    12781 atgcgctgtt tccggggcgt attccgggga aagtaaatgc aggatatcag ggggagtctt
    12841 ttcgacaggc cagatagttt catcttcgcc agtggcatcc agtacattga gaatagcgtc
    12901 aatgcttaac ccaccagtga ccatatcctg caatgtctcg gcttcggtga ctaggtcacg
    12961 caatgaatcc agatcctgcg aggagggctc ttcgttaaaa ggaaaaggat cagcatggcg
    13021 attaagatct tgtccgggag ataacaacgc ctcgcggtat tcctgataaa gtcgggtcag
    13081 ggcatttcct ttttccgcac cttctccggt ggaggaggct ccatgaacag gaatgatatc
    13141 gaatggattt tgtgatgtca gatgctgctg cctgaaccaa tccagatcca gatattcggt
    13201 tattgcttcc gacgtgatgg cagtctgcga tatcgttttt actgtgctca ggtccggaga
    13261 tgatggattg ccatgtacaa ggcactatga agataacccc cattctatca ggtcgccttc
    13321 attcagacgc atacggtgat gaggttccac aatctggtcg tttaccgtac agcaatattc
    13381 ggtagactga ttgataatcc accatgcttc ttcgtggcgg gtcagagtga gggcaatctt
    13441 tacatcatca ttcaccttaa aaacaggaat ccagggagag gtaacggtga agataatttg
    13501 tgttccttca gtcgcgtaaa ttgtgttatc ggcgatcccg tgcgcctgta ttttttggag
    13561 tccccacgta atatttggtt tcatt
//
LOCUS       DADLSE010000099        13509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLSE010000099 DADLSE010000000
VERSION     DADLSE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.099"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(25..477)
                     /locus_tag="JN692_002894"
     CDS             complement(25..477)
                     /locus_tag="JN692_002894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7265162.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            complement(487..885)
                     /gene="yafO"
                     /locus_tag="JN692_002895"
     CDS             complement(487..885)
                     /gene="yafO"
                     /locus_tag="JN692_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7265163.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(888..1181)
                     /gene="yafN"
                     /locus_tag="JN692_002896"
     CDS             complement(888..1181)
                     /gene="yafN"
                     /locus_tag="JN692_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7265164.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(1233..2288)
                     /gene="dinB"
                     /locus_tag="JN692_002897"
     CDS             complement(1233..2288)
                     /gene="dinB"
                     /locus_tag="JN692_002897"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7265165.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2359..3144)
                     /gene="lafU"
                     /locus_tag="JN692_002898"
     CDS             complement(2359..3144)
                     /gene="lafU"
                     /locus_tag="JN692_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7265166.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQRLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            3089..4828
                     /gene="flhA"
                     /locus_tag="JN692_002899"
     CDS             3089..4828
                     /gene="flhA"
                     /locus_tag="JN692_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7265167.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGMLVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            complement(5045..5542)
                     /gene="rayT"
                     /locus_tag="JN692_002900"
     CDS             complement(5045..5542)
                     /gene="rayT"
                     /locus_tag="JN692_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HAY7265168.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5718..6331)
                     /locus_tag="JN692_002901"
                     /pseudo
     CDS             complement(5718..6331)
                     /locus_tag="JN692_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6623..7363
                     /locus_tag="JN692_002902"
     CDS             6623..7363
                     /locus_tag="JN692_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7265169.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7334..8101)
                     /locus_tag="JN692_002903"
     CDS             complement(7334..8101)
                     /locus_tag="JN692_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7265170.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(8307..8885)
                     /gene="lpcA"
                     /locus_tag="JN692_002904"
     CDS             complement(8307..8885)
                     /gene="lpcA"
                     /locus_tag="JN692_002904"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7265171.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            9125..11569
                     /locus_tag="JN692_002905"
     CDS             9125..11569
                     /locus_tag="JN692_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7265172.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGVYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11612..12085)
                     /gene="ivy"
                     /locus_tag="JN692_002906"
     CDS             complement(11612..12085)
                     /gene="ivy"
                     /locus_tag="JN692_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7265173.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            12239..13009
                     /gene="yafV"
                     /locus_tag="JN692_002907"
     CDS             12239..13009
                     /gene="yafV"
                     /locus_tag="JN692_002907"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7265174.1"
                     /translation="MPGLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            13119..13351
                     /locus_tag="JN692_002908"
                     /pseudo
     CDS             13119..13351
                     /locus_tag="JN692_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
     gene            complement(13425..>13509)
                     /locus_tag="JN692_002909"
     CDS             complement(13425..>13509)
                     /locus_tag="JN692_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7265175.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         3222 a         3467 c         3555 g         3265 t
ORIGIN      
        1 cgttattttc attgcaagct ggatttaatg ttgcggttta taacgcatat aaaaattaat
       61 aaaccacgct ccccggcatt caacgcgctg ctgcttaact gtctgaaaac cataacgttc
      121 aaaaaagggt tttgcggtta tgcttgcgtc caccgtaagt tcggattcag acttaatcaa
      181 aggttttaac aaagcgctgg caaccccacg gcgggtgtat tcagggtcaa caaataacat
      241 atcgatatag tgttcaacgc aggtaataaa gccgaccagt tttgcattaa tgactgcaac
      301 ccgcacttgt gatttcgcga gtttctcctt ccagcgagat tcgtcaatct gcgcccaggc
      361 ggcaatttgt tgtggtgaat aatgctgact gacggtcatc gtaaccgctc taatgaaaat
      421 agcgcatagt tgctgaaaat cgccaggctg ataatctctt atttgtatgt tattcataat
      481 ataaattcaa aaacgcatgc gaaacgcttc tgccattttt ccaattttat gcatttggtt
      541 gttatctcga gccagtttat gaggttcagg cttcagaatg gcaatgagca accatgcttg
      601 ctcatcaaac gcaccctgac aatataccaa atgcgcttcg tcattcgttc tgctgaattg
      661 gcgcaactgt ggcggaaatg gattattcac atttgccaga tgaatatgag caactcgctc
      721 aaatttgatt aatggccagg taaacgagtc gtcgtagagt gcatcgcgac caaatatatc
      781 tggcaaaaca ccgtcacgct tataggaaat aaaatccgcc gttaacgcat caagttcctc
      841 tgctgtaagt tgcaggcgaa taagttttgt tttgaatacc cgcatcctta ttccttaaag
      901 tcattgaaat catcatccgt catatcatta agatattgtt tagcgcggcg tgtaatttcc
      961 tctaatcttg cagcacttgg acgaaagcgc gcctttatgg gctttttctc ctcttgttca
     1021 gcaagcatgt ccattaacgc ttcgaatgcg ctggcactta agagatatcc tgcggggcga
     1081 ttattagaaa gaaccgcaac tggttgatca ataaagtatt tagctgggtt tttacgtaac
     1141 tcagtgatat tgaccgattt ttcagcgaga attcgatgca tacagtgata ccctcataat
     1201 aatgcacacc agaatataca taatagtata catcataatc ccagcaccag ttgtctttcc
     1261 atttgcgggt caagcaacgt cacatgcagc cccaccagac gcacaccgcg cccgccgcgg
     1321 cgttcatccc aggttttacg cgcggtggcg attaagtcag ctttattcag ccgcggccag
     1381 acgtgctcct gggtggtctg ctgaaaatcg tcgaacttta atttcacccc ctggcgagca
     1441 atcagtaaat caggttttac ctttgccaga cggcgttcaa gttccggata cagccgctcg
     1501 ataatcgctt cacattcaga ccagtgatga atatcttccg ccatcgtgcg ttccacgccg
     1561 acggattttc gtaaccgttc gctgttaacg tcgcgctcgt caatcccctg actacgctcc
     1621 cacaaaatgc ggccaaattt gccaaagcgt ttaagcagaa tcaccagatc acacttttgt
     1681 acatcaccgc aggtccgcag ccccatcgct tccagttttg ccgccgagac tttgccgacg
     1741 ccggggattt tttccagtgg taaggtttgt aaaaatgccg gaacttctgc cggcgtaatc
     1801 acaaactgac cgttgggttt attcatgtcg gaggcgattt tggcgagaaa ctttactggt
     1861 gccacgcccg cagatgccgt cagttgcagc tcgttgaaga tcgtctggcg gatttcctgg
     1921 gcgatgaggg tcgcagaacc gtggcaatgg acgctatcgg tgacatcgag ataagcctca
     1981 tccagtgaca acggttcaat gcgcgaggtg tagcgcgaga agatttcacg gatatgattt
     2041 gaggcttctt tgtaggcgtc aaagcgcccc ggaagcaagg tgagatgtgg gcataatttg
     2101 agcgccatcc cagtaggcat agcgctacgt acgccaaatt tacgcgcggg ataattggcg
     2161 gtgctgatca ccccccgacg ttcgcggctg ccgccaatag caatagggat atcgcgcagg
     2221 gcgggattgt cgcgcatctc caccgctgcg aaaaagcagt ccatatccac atgaatgatt
     2281 ttacgcattg ctcacctctc aacactggta aagtatacag tgatttcagg ttttgagaaa
     2341 tgcgtaaaga ttcagcattt agcgcattcg ctgcgaaagc acctgctgct tatccagctt
     2401 ttgcaccagc ggctgcacca ctttatcccc atgctgacca aagtattgat acagcgtatc
     2461 ggacgcactt ttggtcagca ccataatctc aatacgccga ttgcccgcgc tttgcggatt
     2521 cttcgcgtcc agcagcatct ggtccgccat tgcgcttacc tgcatcactt tatcttccgg
     2581 cattccggcc tcttccagca cccgacgagc tgaaagcgcg cgatcgcccg aaaggttcca
     2641 gttgttgtag atattgtttt tgtaggccat cgcatcggta tgcccggtaa taataatttt
     2701 gttatcgagc gagtcgaaca ctggcgcgag ctccaccagc aaagttttaa agaacggcat
     2761 aatctgcgcg ctgccgcgtt cgaacatatt gcgattctgg tcgtctttaa tcagcacgcg
     2821 taatctctgg ggaacaatct ccatctccag attcgcttcc atatgcgcat cacgggcaat
     2881 ggtgttgata ctggttgcca actcgccaag ctcggtggcg gatttcttct tcagcagcgc
     2941 cgttttctca ttcacatcac gcgctttttt ctccgtctct tccggcaccg ccacggtggc
     3001 aggttttggc ggatgagacg ggctgatttt atttaacggc gacagcccac caccgttaaa
     3061 aatcgactgc ccgtgcagcg cggcgataat gctttcacgc tcggatttac tgacgctgtt
     3121 gacaatcaac tttatcgtcg tcaccaaaga ggccgagcgt atttccgagg tttctgcccg
     3181 ctttaccctg gacgcgatgc ccggcaaaca gatggcgatt gacgccgatc ttaacgccgg
     3241 attgatcaac caggcgcagg cgcaaacccg gcgtaaagat gttgccagcg aggccgattt
     3301 ctacggcgcg atggacgggg catcgaagtt tgtgcgcgga gacgccatcg ccgggatgat
     3361 gattctggcg atcaacctga gcggcggcgt ctgtatcggc atcttcaaat acaacctgag
     3421 cgccgacgcc gccttccagc aatacgtgct gatgaccatc ggcgacggcc tggtggcgca
     3481 gatcccttcc ctgctgctct ccaccgcggc ggcgattatc gtcacccgcg tcagcgacaa
     3541 cggcgatatc gcccatgacg tgcgccacca gttgctggca agcccgtcgg tgctctacac
     3601 cgccaccggg atcatgttcg tgctggcggt ggtgccgggg atgccgcatc tgccgttttt
     3661 gctgttcagc gccctgcttg gctttaccgg ctggcggatg agcaaacgcc cgcaggcggc
     3721 ggaggcggaa gagaaaagcc tcgaaacgct gacccgcacc atcactgaaa ccagcgaaca
     3781 acaggtcagt tgggaaacta ttccgctgat cgagcccatc agcttaagcc tcggctacaa
     3841 gctggtggcg ctggtggaca aagcgcaggg caacccgctc acccagcgga ttcgcggcgt
     3901 tcggcaggtg atttccgacg gtaacggcgt gctgctgccg gagatccgca ttcgggaaaa
     3961 cttccgcctc aagcccagcc agtacgctat tttcatcaac ggcattaagg ctgatgaagc
     4021 ggatattccg gcggataaac tgatggcgct gccctccagc gaaacctacg gcgagattga
     4081 cggcgtgctg gggaacgacc cggcgtacgg gatgctggtc acctggatcc agcctgcgca
     4141 gaaagcgaag gctctgaata tggggtatca ggtgatcgac agcgccagtg tgattgccac
     4201 ccatgtgaac aagattgtgc gcagctatat tcctgatttg tttaactatg atgacatcac
     4261 gcagttgcat aaccgtttgt cgtcgatggc accgcgcctg gcggaggatt taagcgcggc
     4321 gctcaattac agccaattgc tgaaagtgta ccgggcgctg ctgaccgaag gcgtttccct
     4381 gcgcgatatc gtcaccatcg ccaccgtgct ggtcgccagt agcacggtga ctaaagatca
     4441 tattctgctg gcggccgatg tgcgcctggc gctgcggcgc agcattactc atccgttcgt
     4501 tcgcaagcag gagctgacgg tgtatacgct gaataatgag ctggaaaatc tgctgaccaa
     4561 cgtagtgaat caggcgcaac aggccggcaa agtgatgctc gacagcgtgc cggttgaccc
     4621 gaatatgctc aaccagttcc agagcacgat gccacaggtg aaagagcaga tgaaagcggc
     4681 ggggaaagat ccggtgctgc tggtgccgcc gcagctacgc cctttgctgg cgcgttatgc
     4741 aaggttgttt gcgccggggc tgcatgtgct gtcgtataac gaagtgccgg atgagctgga
     4801 gttgaagatt atgggggcgt tgatgtaaga atccggcatc ggtgcacaat tgccggatgc
     4861 ggcgtaaacg ccttatccgg cctaccgttc aggcacaagt ttgtaggcat gataagacgc
     4921 gccagcgtcg catcaggcat ctgcgcataa ctgccggatg cggcgtgaac gccttatccg
     4981 gcctaccgtt aaaacacgat ttgtaggccg gataagcgta agcgcatccg gcaccagcgc
     5041 acagttataa aatgatacgc tccccggcac ttaaatccgt tatgtctccc gcccaatcga
     5101 tgggatataa ccctcgcgcg acatcgcgat ggaacgttga gaatggccaa tcactcacct
     5161 gctttaccca gccatgtttc tctggattta tataaatata atcaacatga tggcgataat
     5221 cttttgtatt gcgaatggca tgctcccaaa aacgcggttg ccagatattt ttcaatccgc
     5281 aagcatgggt gaactgcttt ttaatctccc gccagcgaga ggaaaaatca tcatcgcttt
     5341 cgggtaatgt ccatatgcaa tgcatatgct ccggcaaaac gacccaggcg ttaatttcaa
     5401 aaggtctgtc tcgcttaacg ttaataatgg cgttacggag cgtctggaac tgggtggtca
     5461 gaagatggct tcgacgattg cgtaaattta ccgtgaaaaa ccatgttccc cccttgatgt
     5521 aataacgacg atattccgac atagcgactc ctttttctca acggtaagag gaaaaggaaa
     5581 ggccgacaat gacgctgtgt tatatcggga aagcggctcg caaaaacgca cagatttgta
     5641 ggtatgataa gacgcgtcag cgtcgcatca ggcatccgtg cacaaccgcc ggatgcggcg
     5701 taaacgcctt atccggccta caaaatcatc tcttccgtca aaatcctgcg cgcgcctaaa
     5761 aaatggtcct gccagaaagg ttcggctaac cggcttaccc gaatggtttc cccggtgcgc
     5821 agtgactcaa taaactgacc gtcgcccaga tacaccccca tatggtcggc aatctcgcgg
     5881 ctgtggatat ggaaaaatag caaatctccc cggcgcaggt cgttattcgc cacaatcgtt
     5941 gcccgacggt agtggtacat ctcattggcc gtgcgcggga gtttcgcctc aaggatcttg
     6001 ttgtaggcat aaaaaactaa cccactacaa tcgaagcctt gatcgggccg cgtgccgccc
     6061 cagacgtaag gcttgccaag ctgctgctcc aggcggtgaa tcgccacttc ggtgatgtta
     6121 tgcaggtgat cgctgcttaa aaagtggttg ttttccgcca gcgcctgcca gcgtctgtca
     6181 ctcgccttga gcggcgcagg aaaccattct ggatgctgtt ttatctgctc ccggttgtgg
     6241 tggcgctggc gagtaaaggg agcaaaacgc ataacgaaaa cacgcctgga tgcaaaaatc
     6301 tgccgagcaa gaacgaagac atgaaagaca taaaccggct gaatgaaggt gagaatttta
     6361 aaagaccgga ggagtaaacg ggattattaa ttgcttattt gcatattact attgagattt
     6421 cataatctta ggaaataaat gatacatgcg cttttcggac tattgaaaac tgcccctgtg
     6481 ctaaatttgt aaacccagcg ccgacaggct tgcgtaaaac tcttttggtg ataatgtgag
     6541 ccttccaaat ccagactatt ccgatttggg atccattttg ccgtcctagc gtgtgtaacc
     6601 gttttatcaa ggaataagca gtatgcgtaa aatcgcatta attcttgcga tgttgttgat
     6661 cccgtgcgtt tcgtttgccg gtttgctggg cagcagtagc tcgaccacgc cagtgagcaa
     6721 agagtataag cagcagttga tgggatcccc tgtctacatc cagatcttca aggaagaacg
     6781 tacgctcgat ctttacgtca aaatgggcga gcaatatcaa ctgctcgaca gttataaaat
     6841 ctgtaaatat tccggcggct tagggccaaa acagcgtcag ggcgatttca aaagcccgga
     6901 agggttttat agcgtccagc gtaatcagtt aaaaccagac agccgttact acaaagcgat
     6961 taatattgga ttccccaatg cttatgaccg tgcgcatggt tatgaaggga aatacctgat
     7021 gatccacggc gattgtgtat ccatcggctg ctacgcaatg accaatcagg gtattgatga
     7081 gatcttccag ttcgttactg gtgcgctggt gtttggtcag ccgagcgtgc aggtgagtat
     7141 ttacccattc cgcatgaccg acgccaatat gaagcgccat aaatattcca actttaagga
     7201 cttctgggag cagctgaagc cgggctacga ctactttgag caaacccgta agccaccgac
     7261 cgtttctgtg gtcaatggcc gttacgtggt cagcaagccg ttaagccacg aagtggtgca
     7321 gccacaactg gcatcaaact acacgctccc cgaggcaaaa taagcgccat tcgcctggca
     7381 taattttttg ccaggtttca ttgcccgtca gcggctgtgt cgcaatcacc gtgaccacat
     7441 cattcggtgt ggtctgcgag ctgaagtcga tttccacatc ctgatccagc aacgttgcca
     7501 cgccaaacgg cgcgcggcgg gtgatccagt gtaaattagt cgagcaatac gccattacat
     7561 agcgcccgtc cgaaagcagc atgttgaaaa cgcccttcgc ccgcagttca tccgccagtg
     7621 aggcgatata tttaaacact gccgccatgt tgcccggcgt gcgcgggtaa cgctgcgtta
     7681 atttatgcag cagccagcaa aaggcttttt cgctgtcggt ttcaccgacc gggcggaagt
     7741 tgccggtttc cagtgatttg tagcccgtca gttgtccgtt atgggcataa gtccagttac
     7801 gcccccataa ctcgcgggta aatgggtgag tattttccag cgccacctcg ccccgattag
     7861 cctggcgaat atgagccacc accgaacagg attttatcgg gtagtcctgg acaagtttgg
     7921 cgatggggga attaaagctg ggttgtggat ctttaaatgt gcgacagcct ttaccttcgt
     7981 aaaaggtaat gccccagcca tctttatgtg gcccggttcc accaccacgc tgtacaagtc
     8041 cggtgaaact aaagcagata tcggtaggga cgttggcgct catcccgagc aattcgcaca
     8101 tgggtaacct ccacaacagc gggagcgcat ggaccccggc gaagattgat ttaagaattt
     8161 tcaggtcgga tgcggcgtaa acgaattatt cggcctacgg ttcgggtaca gatttgtagg
     8221 catgataaga cgcgtcagca tcgcatcagg catcagcgca caagtgccgg atgcggcgta
     8281 aacgccttat ccggcctacg ccagacttac ttaaccatct ctttttcaat taactggatc
     8341 aggatatgga tcactttaat gtgaatctcc tgaatgcggt cggcataacc aaagtgcgga
     8401 acgcgaattt cgatatccgc cgtgccagcc attttaccgc cgtctttacc ggtcagggtg
     8461 atcactttca tccccttctc acgcgccgct gcgatcgctt tgatcacgtt tgcagagtta
     8521 ccggaggtgg agatccccag cagtacatcg ccttcgcgac ctaccgcttc aacgtagcgg
     8581 gagaaaatat cattgaaacc gaaatcatta ccgacgcagg aaatatgact aacgtcagaa
     8641 atagcaatcg ccgggtagcc cggacggttt tcacggtagc gaccggtcaa ctcttcggca
     8701 aagtgcatag cgtcgcaatg ggaaccgccg ttgccgcagg aaagcacttt gccaccggct
     8761 ttaaagctgt ctgctaacag gaccgccgcg cgctgaatgg cgtgaatatt ggcgtcatct
     8821 tttaaaaagt tagccagcgt ttccgccgct tcgttcagtt cgttacgaat aagatcctgg
     8881 tacatgagga tatccttcag cataaatgta atagacaaaa tgcagtgtac cggataccgc
     8941 caaaagcgag aagtacgggc aggtgctatg accaggactt tttgacctga agtgcggata
     9001 aaaacagcaa caatgtgagc tttgttgtaa ttatattgta aacacattgc taaatgtttt
     9061 tacatccact acaaccatat catcacaagt ggtcagacct cctacaagta aggggctttt
     9121 cgttatgatg attttgagta ttctcgctac ggttgtcctg ctcggcgcgt tgttctatca
     9181 ccgcgtgagc ttatttatca gcagtctgat tttgctcgcc tggacagccg ccctcggcgt
     9241 tgctggtctg tggtcggcgt gggtactggt gcctctgacc attatcctcg tgccatttaa
     9301 ctttgcgcct atgcgtaagt cgatgatttc cgcgccggta tttcgcggtt tccgtaaggt
     9361 gatgccgccg atgtcgcgca ctgagaaaga agcgattgat gcgggcacca cctggtggga
     9421 gggcgacttg ttccagggca agccggactg gaaaaagctg cataactatc cgcagccgcg
     9481 cctgaccgct gaagagcaag cgtttctcga cggcccggta gaagaagcct gccggatggc
     9541 gaatgatttc cagattactc atgagctggc ggatctgccg ccggagttgt gggcgtacct
     9601 taaagagcat cgcttcttcg cgatgattat caaaaaagag tacggcggac tggagttctc
     9661 agcttatgcc cagtctcgtg tgctgcaaaa actctctggc gtgagcggga tcctggcgat
     9721 taccgtcggc gtgccaaact cattaggccc gggcgaactg ttgcaacatt acggcactga
     9781 cgagcagaaa gatcactatc tgccgcgtct ggcgcgtggt caggagatcc cctgctttgc
     9841 actgaccagc ccggaagcgg gttccgatgc gggcgcgatt ccggacaccg ggattgtctg
     9901 catgggcgaa tggcagggcc agcaggtgct ggggatgcgt ctgacctgga acaaacgcta
     9961 cattacgctg gcaccgattg cgaccgtgct tgggctggcg tttaaactct ccgacccgga
    10021 aaaattactc ggcggtgcag aagatttagg cattacctgt gcgctgatcc caaccaccac
    10081 gccgggcgtg gaaattggtc gtcgccactt cccgctgaac gtaccgttcc agaacggacc
    10141 gacgcgcggt aaagatgtct tcgtgccgat cgattacatc atcggcgggc cgaaaatggc
    10201 cgggcaaggc tggcggatgc tggtggagtg cctctcggta ggccgcggca tcaccctgcc
    10261 ttccaactca accggcggcg tgaaatcggt agcgctggca acgggcgtgt atgctcacat
    10321 tcgccgtcag ttcaaaatct ctatcggtaa gatggaaggg attgaagagc cgctggcgcg
    10381 tattgccggg aacgcttatg taatggatgc tgcggcatcg ctgattacct acggcattat
    10441 gctcggcgaa aaacctgccg tgctgtcggc tatcgttaag tatcactgta cccaccgcgg
    10501 gcagcagtcg attattgatg cgatggatat taccggcggt aaaggcatta tgctcgggca
    10561 aagcaacttc ctggcgcgtg cttaccaggg cgcaccgatt gccatcaccg ttgaaggggc
    10621 taacattctg acccgcagca tgatgatctt cggacaagga gcgattcgtt gccatccgta
    10681 cgtgctggaa gagatggaag cggcgaagaa caatgacgtc aacgcgttcg ataaactgtt
    10741 gttcaaacat atcggtcacg tcggtagcaa caaagttcgc agcttctggc tgggcctgac
    10801 gcgcggttta accagcagca cgccaaccgg cgatgccact aaacgctact atcagcacct
    10861 gaaccgcctg agcgccaacc tcgccctgct ttctgatgtc tcgatggcag tgctgggcgg
    10921 cagcctgaaa cgtcgtgagc gtatctcggc acgtctgggg gatattttaa gccagcttta
    10981 cctcgcctct gccgttctga agcgttatga cgacgaaggc cgtaatgaag ccgatctgcc
    11041 gctggtgcac tggggcgtac aggatgcgct gtatcaggca gaacaggcga tggacgattt
    11101 actgcaaaac ttcccgaacc gcgtggttgc cgggctgctg aatgtggtga tcttcccgac
    11161 cggacgtcat tatctggcac cgtctgacaa gctggatcat aaagtggcga agattttaca
    11221 agtgccgaac gccactcgtt cacgtattgg tcgcggtcag tacctgacgc caagcgagca
    11281 taacccggtt ggtttactgg aagaggcgtt ggtggatgtg attgccgccg acccgattca
    11341 tcagcggatc tgtaaagagc tgggtaaaaa tctgccattt acccgtctgg atgaactggc
    11401 gcacaacgcg ctgacgaagg ggctgattga taaagatgaa gccgctattc tggtgaaagc
    11461 ggaagaaagc cgtctgcgca gtattaacgt tgatgacttt gatccggaag agctggcgac
    11521 gaagccggta aagttgccgg agaaagtgcg gaaagttgaa gccgcgtaat tgaataacgg
    11581 agccgaaagg ctccgtttct ttatccgcta attatttaaa attaaagcca tccggatggt
    11641 tttccaggct gccggtcaac gccgcgaaca gcaccgtttt accatcaatc gaaagcgcat
    11701 cgttcacatt cagccaggtg agtttctctt gcgacgtttt ctcatcaata gccgagaaca
    11761 gccccgtcat ctgattagat ttctcggacc acatcacagc gatacgttgc gagccacagt
    11821 catgcggttt gcacgcgctc atcacctgat acgtctcatc tcccaacgtt acggtttgtg
    11881 cgggagtata agtaccgcct ttcatcaccc atgcaggtag cttatgccct tgcaccatct
    11941 gattaaacgc agctttggtg gtttcgccct ttgcaaggct gctaatggtt aaatcatcct
    12001 gcgccattgc actggtggcg atgaccagtg cggcgactgt cgttattgcc ttaaacatca
    12061 ttcctcccga gcttatcctg cccatgttat taacctccta aagcctggcg ataacacgat
    12121 aatttgcaaa tcagatattt ccaatatcag catttgttat caatttattc tgcgctaagg
    12181 caactgcctg ataaggtgtt aaagttataa aacccttcgc ataaaggagt tgtacaccgt
    12241 gcctggtttg aagattacgc ttttgcagca accgttggtg tgggtggatg gtcctgccaa
    12301 cctgcgccat tttgatcgtc aactggaagg tattaccggg cgcgatgtga tcgttctacc
    12361 ggagatgttt accagcggct ttgccatgga agcggcagct tcgtcgcttg cacaagatga
    12421 cgtagtgaac tggatgacag ctaaggcgca gcagtgcaat gcgctgattg caggcagtgt
    12481 tgcattacaa acggagtctg gttcggttaa ccgctttttg ctggttgagc cgggcggcac
    12541 ggtacatttt tatgataagc gtcatctgtt ccgcatggca gatgagcatc tgcattataa
    12601 agcgggcaat gcgcgagtga ttgtggaatg gcgcggctgg cgtattttgc cgctggtgtg
    12661 ctacgactta cgttttccag tgtggtcgcg caatctcaac gattatgacc tcgccctgta
    12721 cgtcgccaac tggccagctc cgcgctctct gcactggcag gcattgctga cggcccgcgc
    12781 gattgagaat caggcgtatg tggcgggatg caatcgcgtc ggcagcgatg gcaacggctg
    12841 ccattatcgc ggtgacagcc gggtgattaa tccgcaaggc gagattatcg ctaccgccga
    12901 cgcgcataag gcaacgcgta ttgatgcgga gctgtcgatg gtggcattgc gggaatatag
    12961 agaaaagttt ccggcatggc gggatgcgga tgagtttagg ttgtggtgag tgggggttgt
    13021 gtttagggcc gggggctggg ctagattacg aaagccccta acttaaacac aatattacgc
    13081 cagaagtcac catttccaaa acacagcgtc gctgtaccgt gggttatctg cccaaccgca
    13141 gcgacacgcc ggtgatcaaa atcagcgcca aatggctgag aaaagcggga tttgagatcg
    13201 gcacgggcgt taccgtgaag atctcggagg gatgtttgat cctgcttggg ataacaacga
    13261 agtacaggaa ctgcgcaggg aactttatca ggttaaacag tcagttaagg ggatgcgaga
    13321 cgggatgttt agtgtgctga acgaaagtta aataataaaa agccggaaag atctggaaga
    13381 aatttccggt tattatcgtt gctatatcag gtaatgactc caacttactg atagtgtttt
    13441 atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga gaacgacagt
    13501 gacttccgt
//
LOCUS       DADLSE010000100        13376 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLSE010000100 DADLSE010000000
VERSION     DADLSE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.100"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            359..1189
                     /locus_tag="JN692_002910"
     CDS             359..1189
                     /locus_tag="JN692_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7265176.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIDIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1330..2103)
                     /gene="uxuR"
                     /locus_tag="JN692_002911"
     CDS             complement(1330..2103)
                     /gene="uxuR"
                     /locus_tag="JN692_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7265177.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2318..3778)
                     /locus_tag="JN692_002912"
     CDS             complement(2318..3778)
                     /locus_tag="JN692_002912"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7265178.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3859..5043)
                     /gene="uxuA"
                     /locus_tag="JN692_002913"
     CDS             complement(3859..5043)
                     /gene="uxuA"
                     /locus_tag="JN692_002913"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7265179.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRLGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5383..6726
                     /gene="gntP"
                     /locus_tag="JN692_002914"
     CDS             5383..6726
                     /gene="gntP"
                     /locus_tag="JN692_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7265180.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMIYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGMLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6977..7879)
                     /gene="fimH"
                     /locus_tag="JN692_002915"
     CDS             complement(6977..7879)
                     /gene="fimH"
                     /locus_tag="JN692_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7265181.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7899..8402)
                     /gene="fimG"
                     /locus_tag="JN692_002916"
     CDS             complement(7899..8402)
                     /gene="fimG"
                     /locus_tag="JN692_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7265182.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8415..8945)
                     /gene="fimF"
                     /locus_tag="JN692_002917"
     CDS             complement(8415..8945)
                     /gene="fimF"
                     /locus_tag="JN692_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HAY7265183.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8955..11591)
                     /gene="fimD"
                     /locus_tag="JN692_002918"
                     /pseudo
     CDS             complement(8955..11591)
                     /gene="fimD"
                     /locus_tag="JN692_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
     gene            complement(11658..12383)
                     /gene="fimC"
                     /locus_tag="JN692_002919"
     CDS             complement(11658..12383)
                     /gene="fimC"
                     /locus_tag="JN692_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7265184.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12420..12959)
                     /locus_tag="JN692_002920"
     CDS             complement(12420..12959)
                     /locus_tag="JN692_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7265185.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(13024..>13376)
                     /locus_tag="JN692_002921"
     CDS             complement(13024..>13376)
                     /locus_tag="JN692_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7265186.1"
                     /translation="QAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAIDATRTDVLA
                     LQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARYYAIGEATP
                     GAANADATFKVQYQ"
BASE COUNT         3285 a         3432 c         3190 g         3469 t
ORIGIN      
        1 tatttatttc cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata
       61 aattgataac atatcaaata catcaaaaat accattcaca ggcattatta atatagactt
      121 caatatagca acatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat
      181 aattttaaat gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg
      241 tagagttgat atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta
      301 cagaaaaatt tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat
      361 gtctatgcca ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca
      421 tcatccaaaa gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga
      481 aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt
      541 tgtcgaaatt agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga
      601 tacatgcaga gaattcaatg gtattacaac aacaggacgt atgctctatc gaaacttgaa
      661 aatcacctca gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac
      721 agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga
      781 ggactataca gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg
      841 aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga
      901 gcagccaaga attgatatct atattgatca gataataact gtctcagggg tagcaaattc
      961 ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat
     1021 cattgatata gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt
     1081 tatccccggt gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc
     1141 agaactgttt gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag
     1201 aatgagtact aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt
     1261 aatactcatt gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat
     1321 tataataagt caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa
     1381 ataaatatcg tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg
     1441 ccacattgcc agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt
     1501 gtgatcgccc aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat
     1561 ccacattgga ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct
     1621 gttatgcgtt gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc
     1681 cggcgcgctg gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat
     1741 atcttcgcgg gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg
     1801 cgcctgtaac agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt
     1861 gttgtggctg cctgagctat caagcacata gataccggca ccccggcgca cttccaccag
     1921 ccctttgatc tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat
     1981 ttctgcaatt tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat
     2041 cagatcgcgg atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt
     2101 catggtgttg tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc
     2161 cttatccggc ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag
     2221 cactggaagt tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag
     2281 tttgattgac cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc
     2341 gcgcaccgcg ttcgcacagc tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat
     2401 tctgcggcag atcatcagca aaaataccgc tcaggcccag cagcgctttc acgcggtctg
     2461 cgccctgata ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa
     2521 tggcattacc ctgctcatcc acgccctggg tgtaacgcat ccagccagcc acgcccagcg
     2581 ccaggtgacg ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct
     2641 gcggtaactt ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt
     2701 tgctgaaacg ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg
     2761 acagcgttgg cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt
     2821 tagtcatggt gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag
     2881 agtggctgcc gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga
     2941 actgtgcgcc cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc
     3001 actgacggaa cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa
     3061 tttcctgtaa ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga
     3121 aggtgacgtt ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca
     3181 gtaccgcgac cttcgccaca tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa
     3241 atgctttcag ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg
     3301 acttcggcgc agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt
     3361 gaccgcttgc cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg
     3421 tttgcggacg cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg
     3481 cttctttcat cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg
     3541 tgtacagcag ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca
     3601 ggttaacttc gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca
     3661 gcgcctggtg cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac
     3721 gagaatgatc ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg
     3781 taaactccag aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg
     3841 cagccatgcc ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc
     3901 gcgaacttcg gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt
     3961 cttcaggtcg tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt
     4021 gccttccgct ttacgacggt gttcttcttc aacaatcgct ttcaccactt cgtacatatc
     4081 aacgtcaccg ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt
     4141 ggagcgcaga tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc
     4201 gttgtcagca cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt
     4261 tacggtatca accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag
     4321 gatcgggcgc ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac
     4381 tggaataatc gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc
     4441 tttgtacagc tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg
     4501 cagaccggca atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa
     4561 gcgttcagct gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctagacgttt
     4621 caggatatgc atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc
     4681 gtctggcagc acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta
     4741 gcacacggtg cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat
     4801 ccactgctca tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac
     4861 gacagaccac accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac
     4921 ggaccatact tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc
     4981 ctgacggaca tcggctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc
     5041 catgattttt cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt
     5101 tgtttaggta tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg
     5161 cgtataagtt ctaccttttg gtaagacatg tcaacctgaa ttctgaaatt ggttaaccac
     5221 atcacaagaa tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg
     5281 ttattgatca gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt
     5341 cgcccaaaat aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt
     5401 ctctgggtgg tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat
     5461 tcaatggtgg ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg
     5521 atgtcgctgc tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc
     5581 atcgtggtgt tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag
     5641 atagcacata cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc
     5701 atcggcctta ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg
     5761 ctggttattg ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca
     5821 gtagcagctg ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg
     5881 gtgaatgctt atggcgcgga tatggggatg atttatatct atggcgtact ggtgacgatc
     5941 ccaagtgtaa tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca
     6001 acgccatcat tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc
     6061 gtttcgattc tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat
     6121 atctggctgg taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg
     6181 attgcgatgt ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat
     6241 gacatgcagt gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt
     6301 ctgatcatcg gtgcgggtgg catgctgaag cagaccatca tcgacaccgg cattggcgac
     6361 accatcggca tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc
     6421 actgtgctaa ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg
     6481 attatcagtg ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg
     6541 ctgctggtac tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct
     6601 ttctggttgt tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt
     6661 ctgctggagc tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta
     6721 gcgtaaaaga acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa
     6781 atgtgcccgg acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc
     6841 agtaaagtgc gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa
     6901 gggctaacgt gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca
     6961 atgcgctgta atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc
     7021 ctgcagtcac ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg
     7081 aagttcctac tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg
     7141 tcaactgtac gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt
     7201 tgcccgcatc tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat
     7261 aaacggtaag aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac
     7321 gagcagaaac atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc
     7381 acacaaactg aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa
     7441 ttaatgaacc agctttaatc gccaccccgc ccgcactgct cacaggcgtc aaataaagcg
     7501 ccaccggcca cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg
     7561 tcggaaatgg atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc
     7621 cgccgtaagc cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt
     7681 tatggcaaaa gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag
     7741 gcgcaaggtt tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg
     7801 cggttttaca ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca
     7861 gggtaatagc tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg
     7921 ctaatcactg cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc
     7981 tgtaacggga agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca
     8041 gtattcaatg tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc
     8101 tggtttttat aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac
     8161 gttcccaccg gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg
     8221 gcagacataa gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa
     8281 accgtacacg gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt
     8341 atcgtcgcac ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc
     8401 atgagcatct ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc
     8461 gcagtgacag gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta
     8521 cccggcgtca gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt
     8581 tgctgctcat tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt
     8641 gcaagcaggt tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa
     8701 acggcattac cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca
     8761 atgttgttaa attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca
     8821 gcggccacac tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca
     8881 gccaaaacgc gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt
     8941 ctcatcacgc ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc
     9001 tctggtggca gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc
     9061 tgaacttttc ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg
     9121 ccgctactct ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg
     9181 tgagttagcg tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc
     9241 gccccacgag tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag
     9301 gtattggtat ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca
     9361 taaccacgcc agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca
     9421 ggcgctttaa caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca
     9481 tgagccaata ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg
     9541 taaccgatat tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt
     9601 ccgctattac catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg
     9661 tcttccagca acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt
     9721 gacatgctgt agctggcact ggcatgtcac cactgagatt tactgtcaga acgcagccag
     9781 tggctgaaag gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg
     9841 tttttcgtca ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca
     9901 gcctggaatt gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa
     9961 tacagtgttg atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg
    10021 ttataagcga ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt
    10081 gtttcgatgt tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg
    10141 ctggtcgaat aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg
    10201 ttatagagaa aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta
    10261 gcctgcgtca tatccacaga cagagcgccc agtgccccca tgtttttccc gataccgaaa
    10321 ttaaaagcac gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga
    10381 aggccgtgga gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta
    10441 cggtattctc ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact
    10501 gacgaatagg gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc
    10561 aagtcaccac tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc
    10621 gtactattat aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca
    10681 ataccgtgga tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag
    10741 gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca
    10801 cccaacgtca gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc
    10861 tgccatttat ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg
    10921 tcgcgtaaac gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg
    10981 ctgttacccc caatccgatt ctgtacacta tttccgctga aattataatt gagcaatccg
    11041 gcattaatac cgggatccca taactcagga ggaatataac cacgcgcgcg attactcata
    11101 aatgcctgag ggatcgtcag gttcagtcgc tgctgaccaa catccagatg cgcagtagcg
    11161 tcctggacca ttgtggttaa tggcacacag gcatcatccg ccagcagatt cataccggcg
    11221 acagaagccg tattcagccc catactggcg agttgcgcgc gtgtcaggca gggaacaatc
    11281 ccttgttcac tgtcgcccgt attaaatgtg acatcacgcg ttgccatata accattattc
    11341 aaatagatat cgacgcgata cgtccctggc ggtaattctt gcccatttta aaaacgcgat
    11401 aaatcggcca cagcctgggg atcatccgct aaaaagcgca gattaaaata gaggtcggca
    11461 gatgacaaag gtgcctgtgc ggcaaaagca caggcgacaa cgagtcggac aaaaaaacca
    11521 gccaaacgat gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag tcttaaattc
    11581 agatatgaca ttattcctcc ggtttaaaat cacccggcag tcaattcttt ttattcaggc
    11641 gaaaaattcc ccctgcgtta ttccattacg cccgtcattt tgggggtaag tgcgccataa
    11701 tcatttattg ttcggtaagt aatattgctt cctgcatcag aaggcaattt aaccgtgctt
    11761 tcgcccattg gaggcaccaa tgcattttca agaacccggg ttccggcatt caactctgtt
    11821 accgtcaggt aatagggtgt cgggttaatc agcgtcagag aattcgcgct acgacgaaat
    11881 cttaattttt ctgcggcctg atcgggtggc aacgctaatt tagccgggcg atagtacagt
    11941 ttaatgcggc tgataattgc gagctgtagc gtattctcag tcaattttga tttatccatt
    12001 gacggaatcg ctttaacgtt catccagaat aaactttccc ggtcctgtgg caattggtta
    12061 tttgttgcat caagaatacg taaggtattc tctttttttc ccttcatcgc aaacagagga
    12121 ggcgtcacga taaaacgacc atcctttaca ccatcggcat tttccaccca tgattgaatt
    12181 aaataggtac tattttcatc attatttgtc acggcaagtt gcacttgttt ttgccctgcc
    12241 ggataaatta cgcgagtcgc acctaaggcc actcctgctt cagcgcgtcc ggcaaccatc
    12301 attgccatga acatcaggat acctgccagc aagcagaatg ttatttcctg cgatttcctt
    12361 acattgacgt ttttattact cactgtcctg ttcctgttat cacattatct actgaacaat
    12421 tactgatagg ttaaagagaa ccaggcctgg gcattggcga tgccgccagt aacccgacgc
    12481 ccggtagcac gatatttggc gatgaaatgt agcgaagtag agcctgagta aagccgtttc
    12541 cagtttgctg gaggacgatt aatcggtacg aggtttcctt catcatcaaa caacgctaca
    12601 ccaatattgg aggctatccc tggcccctct cccacggaga gcacatccgg atttttacca
    12661 tccgcgacac cgtgaaacgc cacacccaca cgttcactca ccaccgtgct acattcccgt
    12721 aaatgaataa caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa ccggttactg
    12781 ctgatttgcc ccatattgac cgtcatttgt ttatcaccgg cttcaatccg gcaagtttcc
    12841 gcaataatga cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt ccatttattt
    12901 ccggccagag caaacattgg cagcaacgac gctaacagaa ataatctctt ccttttcatc
    12961 gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt gaacctgggt
    13021 aggttattga tactgaacct tgaaggttgc atccgcatta gctgcacccg gggttgcctc
    13081 gccgattgca taataacgcg cctggaacgg aatggtgttg gtaccgttat tcagggttgt
    13141 ttgtgcacta aatgtcgcac cgtccagcgt cagagcattg cctgttctgt ccaggatctg
    13201 cacgccaacg tttgttgcac tacctgcagc agaactctgc agagccagta catcagtacg
    13261 cgtagcatca attgccgtac ctaagaaggc aacagcggct tttgtggcaa cagtggtatc
    13321 gcaatcattc agctgaatgt taaaaccaac ggcagagctg gttgctccag cctgct
//
LOCUS       DADLSE010000101        13239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLSE010000101 DADLSE010000000
VERSION     DADLSE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.101"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..328)
                     /locus_tag="JN692_002922"
     CDS             complement(<1..328)
                     /locus_tag="JN692_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265187.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNVRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIATI
                     FEEYLME"
     gene            complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN692_002923"
     CDS             complement(645..1469)
                     /gene="iclR"
                     /locus_tag="JN692_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7265188.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1669..5352
                     /gene="metH"
                     /locus_tag="JN692_002924"
     CDS             1669..5352
                     /gene="metH"
                     /locus_tag="JN692_002924"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7265189.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKWIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYARDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGKDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5572..7203
                     /locus_tag="JN692_002925"
     CDS             5572..7203
                     /locus_tag="JN692_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7265190.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQGLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN692_002926"
     CDS             complement(7294..7971)
                     /gene="pepE"
                     /locus_tag="JN692_002926"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7265191.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFGVTQTWDD
                     YTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGLLAPI
                     TDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTNALPE
                     GHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGEEAVP
                     LEAGHRF"
     gene            complement(8353..9582)
                     /locus_tag="JN692_002927"
     CDS             complement(8353..9582)
                     /locus_tag="JN692_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7265192.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            complement(9634..10422)
                     /locus_tag="JN692_002928"
     CDS             complement(9634..10422)
                     /locus_tag="JN692_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7265193.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            complement(10433..11230)
                     /locus_tag="JN692_002929"
     CDS             complement(10433..11230)
                     /locus_tag="JN692_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7265194.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            complement(11296..11790)
                     /locus_tag="JN692_002930"
                     /pseudo
     CDS             complement(11296..11790)
                     /locus_tag="JN692_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(11790..12197)
                     /locus_tag="JN692_002931"
     CDS             complement(11790..12197)
                     /locus_tag="JN692_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7265195.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(12207..13013)
                     /locus_tag="JN692_002932"
     CDS             complement(12207..13013)
                     /locus_tag="JN692_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7265196.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(13083..>13239)
                     /locus_tag="JN692_002933"
     CDS             complement(13083..>13239)
                     /locus_tag="JN692_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7265197.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
BASE COUNT         3163 a         3420 c         3482 g         3174 t
ORIGIN      
        1 tttccattag gtattcttca aatattgtag caatggtttg agtgagtgtc gtgttttcgt
       61 ttttattaat tatcgtaatt tctttcttat cagcttgtaa gacggacgtg gaaaatttaa
      121 tgacaaaatc cttgttgcta aagatatgac gaacattttg tctacagttc tctgagaagc
      181 tttttaatag aggcgtcgcc agctccttgc cagaaaattt atcctcgagt tctttataaa
      241 acaattcact cagggtttgg tgttcatttg tctgggctgt attattaata tttgcagaga
      301 aagaactacg aggaatacga gtaatcattt aaatatctca ttgtttattg atgtcttgaa
      361 ttttaactat cagaacagtg aaaaaattta atatgattac aactaaaaaa atatcataaa
      421 tcgctcaatc tcataatgca gccgtaaaag tttcggtgga atgagatctt gcgattttgt
      481 taataattaa gtggtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg
      541 tatgacatta agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac
      601 tcaggtatga tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg
      661 tacgccagcg tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcga
      721 tcatcggtaa tacgtgaaat cggtccggaa atagaaattg cggcaaacgg ttcgcggtgc
      781 tcatcgaaaa tacacgctgc aaggcaacgt agccccagcg catgttcctc atcgtcaaat
      841 gaataacccc gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccaga
      901 gttgcgtggg tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg
      961 ctcagttggg ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg
     1021 ataggcgcgg acattcgcat caggtgggta cactgtacct ggtcgataat aatcgcttcg
     1081 tgatcgcttt gatcaagcac cgccatattg accgtttcgc cagactcttc cattagattg
     1141 cgcaggatag ggtgaacaat tgctaacaaa ttacggctct ggagaaagct gctgccgacc
     1201 ataaaggcat gtgcgccgat tgcccagtgt cccagttcgc cgacctgacg cacgaaaccc
     1261 tgctgttgca tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc
     1321 gccagctctg tgagtgccac gctgccattg gattcggcaa tccactccag taatttcagg
     1381 ccacgcgtta aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg
     1441 ccgcgtttcg cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatt
     1501 attttcattt ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt
     1561 tgaacaaatc tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg
     1621 gtttgttgaa tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa
     1681 gtggaacaac tgcgtgcgca gttaaatgaa cgtattctgg tgttggacgg cggtatgggc
     1741 accatgatcc agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac
     1801 tggccatgcg acctcaaagg caacaacgac ctgctggtac tcagtaaacc ggaagtgatc
     1861 gccgctatcc acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc
     1921 aactccacga ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac
     1981 tttgcggcgg cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag
     2041 aaaccgcgct acgttgccgg tgttctcggc ccaaccaacc gcacggcgtc tatttctccg
     2101 gacgtcaacg atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctatcgc
     2161 gaatccacca aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc
     2221 gacaccctta acgccaaagc ggcggtcttt gcggtgaaaa cggagtttga agcgctgggc
     2281 gttgagctgc cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc
     2341 gggcagacca ccgaagcatt ttacaactca ttgcgccacg ccgaagcgct gacctttggc
     2401 ctgaactgtg cgctggggcc cgatgaactg cgccagtacg tgcaggaact atcacggatt
     2461 gcggaatgct acgtcaccgc ccacccgaac gccgggctgc ctaacgcctt tggtgaatac
     2521 gatctcgacg ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggctttctc
     2581 aatatcgtcg gtggttgctg cgggaccacg ccgcaacata ttgcagcgat gagtcgtgca
     2641 gtagaaggat tagcgccgcg caaactgccg gaaattcccg tagcctgccg tttgtctggc
     2701 ctggagccgc tgaacattgg cgacgatagc ctgtttgtga acgtgggtga acgcaccaac
     2761 gtcaccggct ctgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcgctg
     2821 gatgtggcgc gtcagcaggt ggaaaacggc gcgcagatta tcgatatcaa catggatgaa
     2881 gggatgctcg atgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg
     2941 gatatcgctc gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt
     3001 ctgaagtgga tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac
     3061 gcctttatcc atcacgcgaa attgttgcgt cgctacggtg cggcagtggt ggtgatggcc
     3121 tttgacgaac agggacaggc cgatactcgc gcacggaaaa tcgagatttg ccgtcgggcg
     3181 tacaaaatcc tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac
     3241 atcttcgcgg tcgccaccgg tattgaagag cacaacaact acgcgcggga ctttatcggc
     3301 gcgtgtgaag acatcaaacg cgaactgccg cacgcgctga tttccggcgg cgtatctaac
     3361 gtttctttct cattccgtgg caaagatccg gtgcgcgaag ccattcacgc agtgttcctc
     3421 tactacgcta ttcgcaatgg catggatatg gggatcgtca acgccgggca actggcgatt
     3481 tacgacgacc tacccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgt
     3541 gacgatggca ctgagcgttt actggagctt gccgagaaat atcgcggcag taaagcggac
     3601 gacactgcta acgcccagca agcggagtgg cgctcgtggg acgtgaataa acgtctggaa
     3661 tactcgctgg tcaaaggcat aaccgagttt atcgagcagg atactgaaga agcccgccag
     3721 caggctacgc gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc
     3781 ggcgatctgt ttggcgaagg caaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc
     3841 atgaaacagg cagtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggtaaa
     3901 accaacggca agatggtgat cgccaccgtg aaaggcgatg tccacgacat cggtaaaaat
     3961 atcgttggtg tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg
     4021 cctgcggaaa aaattctccg tatcgccaaa gaagtgaatg ctgatttgat tggcctttcg
     4081 gggcttatca cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag
     4141 ggcttcacca ttccgttact gattggcggc gcgaccacct caaaagcgca cacggcggtg
     4201 aaaatcgagc agaactacag cggccctacg gtgtatgtgc agaatgcctc gcgcaccgtt
     4261 ggtgtggtag cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc
     4321 aaggagtacg aaaccgtgcg tattcagcac gggcgcaaga aaccacgcac accaccggtc
     4381 acgctggaag cggcgcgcga taacgatttc gcatttgact ggcaggctta cacgccgccg
     4441 gtggcgcacc gtctcggcgt gcaggaagtg gaagccagca tcgaaacgct gcgtaattac
     4501 atcgactgga caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg
     4561 gaagatgaag tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg
     4621 gataaattaa gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca
     4681 aaccgtgtgg gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac
     4741 gtcagccacc atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct
     4801 gacttcgttg cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact
     4861 ggcgggctgg aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac
     4921 aaaatcatgg tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat
     4981 gagcgtgtgc gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa
     5041 ctgatccgcg aaaactacca gggcatccgt ccggcaccgg gctatccggc ctgcccggaa
     5101 catacggaaa aagccaccat ctgggagctg ctggaagtgg aaaaacatac tggcatgaaa
     5161 ctcacagaat ctttcgctat gtggcctggt gcatcggttt cgggttggta tttcagtcat
     5221 ccggacagca agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc
     5281 cgccgtaaag gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat
     5341 gacgcggact gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag
     5401 tgccctgtct ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag
     5461 ccggaaaaac gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt
     5521 tggtcattca attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg
     5581 ctgcttcacc tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc
     5641 ggcgtaatgc gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa
     5701 aagccgctcg ccttttgcgc ggggatcggc gttaccgcac tggtacagag cagtaatgcc
     5761 accaccatgc tggtgacctc gttcgtcgct cagggtctgg tagccctcgc accggctctg
     5821 gtcattgtgc tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac
     5881 ttatcctggc tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa
     5941 cagtcacgcg ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttgctggcg
     6001 ctggagttga ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc
     6061 tttgcctcgc tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt
     6121 atcagctact ccagccttgc tgctgtactg ctgaccgcga ctctgaccgc tgcaggcatt
     6181 atctccttcc ccgtggcgct ctgtctggtg attggagcta acctcggttc cggcctgctg
     6241 gcgatgctca acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg
     6301 ctgtttaagc tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca
     6361 atggggaagc tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac
     6421 cttgtacgtt gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg
     6481 attattcgcg atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc
     6541 gcgctggaca cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc
     6601 gacgccatgg aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag
     6661 gagaaagagc tgcgtaagct ggcagatgat atcaacgttc tctacaccgc cattaagctg
     6721 tatctggcgc ggatgccaaa agaagagttg gcggaggaag agtcgcgccg ctgggcggag
     6781 atcatcgaaa tgtcgctcaa ccttgaacag gcctccgata tcgtcgagcg catgggcagc
     6841 gaaattgccg ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa
     6901 ctggatgcgc tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc
     6961 tctggcgatg tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt
     7021 aatcgccgct attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt
     7081 gaaaccagtt cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt
     7141 tgttcggtgg cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat
     7201 taatattgtg aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga
     7261 acaagatgcc ggatcatgca tccggcaatt cacttaaaaa cggtgaccag cttccagcgg
     7321 aaccgcttct tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg
     7381 acctttactc actgtgatcc agttaccttc cggcagacca ataatcgtca gttctggcgc
     7441 gacgaccagc agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag
     7501 cgcgttggtg aagtgcgggt tgatttgcaa cgggaacaga tttagcgcat cgaaaccttg
     7561 cggatcgaca atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc
     7621 tgcgctccag ccaatataca gcgcgccacg tttaaccacg tcagtaattg gagccagcag
     7681 cccgcgctcg cggcactgtt tcagcaactg gaaagtatta ccgccgccga caatcacaat
     7741 ttccgcattt tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagaaac
     7801 acccagcgga gcgagaaccg cagccgtttt ctctgtgtaa tcatcccagg tctgcgttac
     7861 gccaaacacc gctgagcggc gaccctgcaa ctgttcagca attagcggca gtgcatgttc
     7921 cagccaggct ttacccggca gcgtcgagtt actcaataaa agcagttcca ttacttctcc
     7981 ggttagtaag aaatcacgga aggcatgcta ccactgcgaa tttaaccact tactgacatg
     8041 gctcacgatg ttacgtattg caccgggcgc tgagactgat gacaaacaca gcattgcctg
     8101 atgcgctacg cttacctggc ctaccagata actgcaatat attgaatttt caaaattatg
     8161 tagaccgaat aaggcttcat tccgcatccg gcataaacaa tgtgtacttt gccaccaatt
     8221 tgagcgcccc ttaaggcgct gatatttttt atccttctcg tgcaaattgt gctgcttcag
     8281 caatcagccg cacatccggt cgcgaagttg ctggggcgac cagccagcga catgcagagt
     8341 taagtagcgc gattaatcat gggaaattgc ctctgcatgg gcgagcaggt attgctccgc
     8401 ctcaccggac cagatcccct gatgacgcgc cagaatcgcc gccagtgctt gatcctgaat
     8461 ctgcgtgagt gacgtcagcg gcaggtattt cccggtatac accagctttt tgccgccgcc
     8521 gacggcaggc aattcaagcg tggtttcgcc cgcggcattc agcccaagaa tatgtgttac
     8581 cacttttgcg gcctgcactt ttttctcttc gatcagtttg accgccgcgc gcatgtcgtc
     8641 ggtattgccg cctgacgtgc ccacgtagtg ggtaaatgca taatgcacat cgtagaaatt
     8701 aattggcgcg ctgaaatgtt tatcctgcgg tccggcgaag aaattcaggc aaccatctgt
     8761 cgccagcaag gaagaggcga gagtcaccag tccttcatta ggcacaaaga cgaaaatatc
     8821 atcgaagccg tgaccgccac tcagcgccat taacgtatca aatgctgcat cggtggcatt
     8881 gagataatga atcagtgttt gcggttctga tggatagtgc ttgcgcgcat aactcaattt
     8941 gtcgttgtcg gtatcggtaa tgacgagcag cgacgggtta actggtccat gcagcgcata
     9001 atcaatcgcc aacagtccca ttggtccggt gccgccgagg atcagcgtgc gcccttgcgg
     9061 gcgaatcccc atcgtgtggt tataactacc ttcctgaaga tgatagttgg cgttgaacgc
     9121 gccaatcacg caggaaagcg gttcaaccag tgagccttca aaataggttt cgccgtcgta
     9181 tgccagcagg caatcttgtt ccatgacctc gttgggaata accacatgcg tcgcttcgcc
     9241 gcctacccac gggaaggagt agccggggca atccgggcgg tcggggagtt gcaggttggc
     9301 ctgaatcaca taacgctgac ccggctggaa tttgtgctgc cactttttac ccacggccag
     9361 aatatcgccg caaaattcgt ggccgatgat gattgggttg gtcgccacat cgtcgggtac
     9421 ttttttatgg ttttcaccca gattggcctc tttccaggaa gagaggcaca ggctgtcagt
     9481 gaccaccgtt gcgaggattt catcctcctg catttcagga aggtcaaagg tttccaggcg
     9541 taaatcacgt ttaccataaa gacgcagagc tgttgttttc ataaaccacc tcatgttgag
     9601 cgatggcctc ggcagtcgtg ccggggcgaa gaatcaggac aggaagccca gagcattgcc
     9661 gataatccct aagccaaaca gggcgaaaat cagccatacc gggttaattt ttttgttgag
     9721 cagacgaacc attagtagcg tcagaccgag cgccagcaaa ccagggcaaa gttggtcgag
     9781 aatgttctgc acggtcatgg tgacggtgga gccatcggcg gcatgcgttt gtgaaaccac
     9841 caacggtacg ttgattgacg tccatttggt caccagcacg cccatcacaa acagtccgag
     9901 aattgacgcg ccttcggtga gtttttgcag cacattcccg cccatatcgc tgacgatatt
     9961 cacccctttg cgaaagccga gctgtaggcc ataccacttc atcgccagac gcaccgcgtt
    10021 gaaaataaag aagaacagca gcgggccgag aatgttgccc gaaagtgcca gagatgcgcc
    10081 gagcgcggcg gtaatcgggc gcagcgttcc ccagaccagt ggatcgccaa ctcctgccaa
    10141 tggtcccatc agaccgactt tgatgccgtt gatggtaccg tcatcaattt ccgcgccgat
    10201 gaccgggcca catacggctg gcgtggtatt gaagaacacc aggtgtcgcc tgagcgccgc
    10261 aacctgatcc tcttttaatg ggtaaagtcg cttgatggcg gggatcatgt cgtagcaaaa
    10321 gcccagcccg tgaatacgtt caaagttaaa ggacgcctgt tgcaggttgg agcgcagaaa
    10381 catgctcacc agatcgctgc gtgtaatttt tctctgttcc atgatggctc cgttaatcgt
    10441 caagttggtc aagggcggtg gaggcggtgg cctggagttg aggttcagct ttacgccact
    10501 gtggatttag ctggatgtag accagggcca tgatcacgcc gacgccaccg aacgccagca
    10561 ggctgagatc gaggtagcca cctgcgagga aaccgaggaa aaagaaaggc atcaaatatt
    10621 tcacgcccat catacgtagc accatggcgt aaccgaccac cacgataaaa ccgccagcaa
    10681 tctgtagtcc tcgggtcaca aattcgggaa tggcgtttaa catattgctg accatatcgg
    10741 cgctgacgaa cagtgagaca atcagtgccg gaatggcgac gcgcagcgcc tgcacgccaa
    10801 gcgcggagac atgcagaata tcgagagtgc gaaaccgcgc ctcttctgct gctttatccg
    10861 ccgcgtgctg gaacactacg gtgatggtac gggcaaacac cgtcagcact tggcctgccg
    10921 cagccacagg caacgcgatg gcgattccgg tggcgatgct ttgctgacca acgataacca
    10981 gaatggcgga gattatgctg gcgagtgcag aatccggaga ttgcgccgcg ccgacgttca
    11041 tccagccgag agctatcaat tccagcgtac cgccgagtat tattccggtt tttaaatcac
    11101 cgagaattaa accaatcacc gtacaggcaa ttaacgggcg atgagtctga aattcatcca
    11161 gcacgctgcc cattccggca atacaggaaa aaagaaatat agcaatgatt tgtagggtac
    11221 ttatttccat gatttgtcac cttttaagtg tcgtcatccc tgcctgttaa agcatatgta
    11281 cacaggcgct attatttaat ttgcgattaa ctgttcgttt attttgtcga taatattgat
    11341 tgagggatct gacgctacga cgcgtaaatc cagtatgaca ccaagattat ttaactcatg
    11401 aaatgcgtta atatcatcat catctaatga aaccgctttg gttaactgtt ttttaccggg
    11461 ccgctaggcc atgccgccaa tattcagggt gcctattttt accccctggc gaaccatcgc
    11521 taaggtatcc tgtgggcgag tgaaaagata aaaaacggtt tcgtcctgat attgcgggtt
    11581 gtgataaacc gcgacggctt tttcaatatt gaccacgtta accttcattc ccggtggcgc
    11641 agcctgacgc aataatgtcc ggcgcacttc atcgttataa acttcgtcat tgcagataat
    11701 aatgcgctgg gcgttagcca ctttcgacca gacggtagtg acctggccgt ggatcagacg
    11761 gtcatcaatg cgggcgagcg taatgttcat cagaaatcct cctctgtcgt tgccagttgt
    11821 ttccagacaa cgcaggtttg ctttgcgatt tgcgtcaggt gttcacacag ttcatcaaca
    11881 ttcatgctgt cctggttatc aaccagttcg agcgccagcg gcagagaaag gccgctgatt
    11941 acccgcaaac gaggattgcg catcgcaagc attgctgccg cattccacgg gctgccgcac
    12001 tgtaaatcca cggcaatcag ccactcatct tgattgtgaa tgcttactaa cttttccagc
    12061 tttaccgcaa tgtctccggc gttttcaccg ggcacaaatt caacggcttc aacattggca
    12121 ttgccgtaaa ccatctgaac cgattccagc atggcgcaag ctaatttgcc gtgggcacaa
    12181 aaaatggcgt tgaccataaa gcctccttag ccgcgcgttt tgccgcccgc aatgttagtg
    12241 gttactccgg tgatgtagct ggcgcgttca gacaacagat aacaaacaaa atcagctact
    12301 tctgctaatc ttccggcgcg cccaatagga atggcgtttt tggtatagcc ttcacgcagc
    12361 tgctcgacgg tgatattgcg cgtccacgcc agcgcctctt catattccgg cgtgcgtagc
    12421 ccggtttttt ccagaatccc gggcgcgata ccgaccacac ggataccgtg tttacccagc
    12481 tctttcgacc aggagcgcgt gaagctattg agcgcggctt tggtagcggc gtaacagctt
    12541 tgtccttctg agccttccag cccactttct gaggaaacat tcacaatcac gccatcatgt
    12601 tgtttgacca tctgtcgcgc caccgcctgc gacatcagaa aaacaccttt ctggttgata
    12661 ttgaccattt tttcgaatgc agcttcgttg agttcatact gcccggcagg cgctttctca
    12721 tcgaccagca gacgcgggaa attgaccccg gcgttattga ccagaccgtc gatgcgacca
    12781 aaacgctgga taatttccgc taccgtatga tttacctctt tggtgctaga aatatcggtc
    12841 ggccaaaact gatagccttt atgaccttca tattgaccat cgccaccgtg aatatcgacc
    12901 atctgtacat tcgcgccttg tgctaataat tcctctacaa tcgccagacc aataccggat
    12961 gcgccgccgg tgacaataat gattttatcc tgcaaattta accacgtttg cataacagac
    13021 tcctgatttt agataatagg gacccgccgt aatttttatt acggtaaaca tcgggaagat
    13081 ttttatttca gtaatagttc agctgtgctg ctattcgtta ccagacaatt aatatatttt
    13141 ccacgcagtg caccaataat tccgctgtat ttttcttcac tcatggcaat gccaatggaa
    13201 tatcgcgcct gctttaattt attcatttcg atagagagc
//
LOCUS       DADLSE010000102        12936 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLSE010000102 DADLSE010000000
VERSION     DADLSE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.102"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(565..1656)
                     /gene="ychF"
                     /locus_tag="JN692_002934"
     CDS             complement(565..1656)
                     /gene="ychF"
                     /locus_tag="JN692_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7265198.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1773..2357)
                     /gene="pth"
                     /locus_tag="JN692_002935"
     CDS             complement(1773..2357)
                     /gene="pth"
                     /locus_tag="JN692_002935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7265199.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2635..2913
                     /gene="ychH"
                     /locus_tag="JN692_002936"
     CDS             2635..2913
                     /gene="ychH"
                     /locus_tag="JN692_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7265200.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="JN692_002937"
     CDS             complement(2968..4647)
                     /gene="dauA"
                     /locus_tag="JN692_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7265201.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSTLVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4772..5719)
                     /locus_tag="JN692_002938"
     CDS             complement(4772..5719)
                     /locus_tag="JN692_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7265202.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="JN692_002939"
     CDS             complement(5870..6721)
                     /gene="ispE"
                     /locus_tag="JN692_002939"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7265203.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     SLHRAML"
     gene            complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="JN692_002940"
     CDS             complement(6721..7344)
                     /gene="lolB"
                     /locus_tag="JN692_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7265204.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7558..8814
                     /gene="hemA"
                     /locus_tag="JN692_002941"
     CDS             7558..8814
                     /gene="hemA"
                     /locus_tag="JN692_002941"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7265205.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8856..9938
                     /gene="prfA"
                     /locus_tag="JN692_002942"
     CDS             8856..9938
                     /gene="prfA"
                     /locus_tag="JN692_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7265206.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9938..10771
                     /gene="prmC"
                     /locus_tag="JN692_002943"
     CDS             9938..10771
                     /gene="prmC"
                     /locus_tag="JN692_002943"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7265207.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10768..11160
                     /locus_tag="JN692_002944"
     CDS             10768..11160
                     /locus_tag="JN692_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7265208.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRHMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11164..11974
                     /locus_tag="JN692_002945"
                     /pseudo
     CDS             11164..11974
                     /locus_tag="JN692_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12010..12864
                     /gene="kdsA"
                     /locus_tag="JN692_002946"
     CDS             12010..12864
                     /gene="kdsA"
                     /locus_tag="JN692_002946"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7265209.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3248 a         3233 c         3342 g         3113 t
ORIGIN      
        1 agtggttaaa aaatttggtg aagatttttt gtaataatcc taattagtta tgggattttc
       61 gcttgatgta tctgcataac aaaaacaata tgcgccattt ttgcattact aataagaaaa
      121 agcaaaaaac atcgatattc atttttggtc aatggatttt ctggcctatc gttattattg
      181 tgctactggt tatcattttc atcttgccag gatcgcacgt aatcacgtta ttcggggact
      241 ttatctgcgg gatattaccc ttcaggtaag tccttttctc gccagcatat tggagcgaga
      301 tcaatattga tcagctgtga agagggctga tgtgtagtgg aacaaaaact gacgttatat
      361 cactgctaat gagatatcag tcatctgaac acttatgtct ttaattattc tcgtggttca
      421 ctgtaggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac tcggtgaaat
      481 gaaaaatcca cgcaattgcg tggattttat atacttttgc gctcttcatg agatttagcg
      541 aaacctcatg agacaataaa ttaattagac gttgaaaagg aagttcatca catcgccatc
      601 tttcacgatg taatctttac cttctgcacg cattttgcct gcttctttcg cgccttgttc
      661 acctttgtaa gtgatgaaat cttcaaacga gatggtttgt gcacggataa agcctttttc
      721 aaaatcagta tggattttgc ccgctgcctg cggcgcggtt gctccaaccg gaatggtcca
      781 tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc aggttcagca gcttataacc
      841 ggcacggatc acacggttca ggcccggttc ttccagccca agctcctgca taaactcgtc
      901 acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa ccggaaccac
      961 aacagaacct tctttcgccg cgatttcacg cacctggtca agatatgggt tgttttcaaa
     1021 accgtcttcg ttgacgttgg cgatgtacat tgttggtttc agcgtcagga agctcaggta
     1081 acgaatagcc gctttctctt cagcgcttaa atccagcgcg cgcagcatac ctgcgttttc
     1141 cagctggggc aggcattttt ccaggaccgc cagctcagct ttcgcgtctt tatcgccacc
     1201 tttggctttc ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt ctgccagcgc
     1261 cagttcggtg ttgataactt caatatcgtc agccgggtta actttgccgg aaacgtgaat
     1321 gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt cacggatgtt
     1381 ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg ccttttacca gaccggcgat
     1441 atcgacaaat tccatggtcg tgggaagcgt acgttgcggt tttacgattt cagccagttg
     1501 atccaggcga gggtcaggca ttggtacgac gcctgtgttc ggctcaatgg tgcagaatgg
     1561 aaagttggcc gcttcaatac cggctttggt cagcgcgttg aacagggtag atttcccgac
     1621 gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt taaatcacct taatatctta
     1681 ataatcaacc tgttattgct aacagattgc agaaatggaa ataactttgc ctattataca
     1741 cggcactcgg caaaaatgcc gcagacaacg acttattgcg ctttaaaggc gtgcaatcgg
     1801 ttcgttgctt tggtcaagcc atctgtaaac cacatttcag tacaacgcgc cgcttcgtcg
     1861 atggcttcat caattaactt ctgttcacta acaggcggtt tgcctaacac aaaaccgaca
     1921 actttatttt tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa gttagggtta
     1981 ttacccaatt tactgatgat gtctttcagt ccattgtgac caccatggcc accgcccaac
     2041 ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt gggccaccag aatttcgtcc
     2101 ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag attcataaat
     2161 gtagtcggga ctaacaggcg gacatcttcg cctccaaggg tgactcgcga agtataacca
     2221 aagaatttag cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa gtcaacgaac
     2281 caggcaccag cattatgtcg cgttgcggcg tattcagcac cggggttcgc caggccgaca
     2341 atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg cgtagtttac
     2401 tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc
     2461 tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat
     2521 aagggttgta attgtgatta cgcccgcaca taacccacag ggtgttgtct atactttaca
     2581 cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa
     2641 cgcaaaaacg cttcgatact cggtaacgtg ctcatggggt tgggtctggt ggtaatggtg
     2701 gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc
     2761 gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt
     2821 gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgtcacta tgacaaacgt
     2881 tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca
     2941 gtttgcgctg actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg
     3001 gaagaacgcg aggcgtcccg ggatcggttg aatgcctgcg cgagccatag ttcgcagtgg
     3061 ctggaattcc acgttgcaca cgcgcagttc acatccttcg ggcagacgct tcacaaaacg
     3121 ctggaacgca tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac
     3181 aatccgtttg ccttcaagac gtgactctag gtccgtgaat aagccttcag cagcagcaaa
     3241 aaacagcggg ccaataacgc gcagaaccag gacatcgtct ggaacatcta cgactaccgg
     3301 tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc
     3361 cacgctgatg gcaataacca tatcaaacag cacggtcagt gacatacaca gcagcatgac
     3421 gatgatgtca tctttcggcg catgacgcag caagtcgacc actttgtgcg cttcactcat
     3481 gttccacgcc accatcaaca gcagggctgc catggcggaa agcggcagcc aggagagcag
     3541 tggtgccagt accagcaggg cgagaataac cagagtagag tggatcaccg ccgagatagg
     3601 ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagcag tagcggtaat
     3661 accaccaaag aatggagcga tgatattccc cagcccctga ccgaccagtt cactattcgc
     3721 cttgtgctta gtcccggtca taccatccag taccacagcg cagagcagag attcgatcgc
     3781 cccgagcatt gccattgaga atgccgcagg cagcagggtg cgaatagaat cccaggttag
     3841 cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat
     3901 accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac
     3961 atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag
     4021 gtgaccgggt aaacgaatgc ccagacgcgg ccagaaaacc agaatgccaa gcgtcacaat
     4081 gccaatggca gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt
     4141 ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat
     4201 ggtaccgatg gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata
     4261 ctcaatcaga cgaccaaagc gtgccagccc catcagaatc aaaaagattc ccgacaacaa
     4321 ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgaa acgggataga gaattaccac
     4381 aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca cccgttagag caatgacaat
     4441 ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc acaccactac caatagccaa
     4501 cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt
     4561 aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat
     4621 cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc
     4681 aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac
     4741 ccgccgcagc gggtctttga gccgggttcg attagtgttc gaacatggca gagatcgatt
     4801 cttcgttgct gatacgacga atcgcttcgg ccagcatacc tgacagggtc agagtacgca
     4861 cgttcggcag tgatttgatt tcatcgctca gcggaatggt atcgcagaca acgacttcat
     4921 caattacaga gttacgcagg ttgttcgccg cgttgccaga gaagatcggg tgagtcgcgt
     4981 acgcaaatac acgtttagca ccacgttctt tcagcgcttc agcagcttta cacagcgtac
     5041 cgccagtgtc gatcatatca tcgaccagta cgcagtcacg acctgcaacg tcaccgatga
     5101 tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt gccatatcgg
     5161 tatcgttcag cagcttagcg atagcgcggg cacgcacaac gccgccgatg tccggagaaa
     5221 ccacaattgg gttatccaga ttcagctgca gcatgtcttc cagcaggatc gggctaccaa
     5281 atacgttatc aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc agatccactg
     5341 tcagcacacg gtcaacaccg acgctggaga ggaagtctgc aaccactttc gcagtgattg
     5401 gtacacgagc ggaacggacg cgacggtcct ggcgcgcata gccaaagtag gggataacag
     5461 cggtgatacg acctgcggaa gcacgacgca gggcatcaac cataacgact aattccatca
     5521 ggttgtcgtt agtaggggca caagtggact ggatgatgaa aatatcacca ccgcgtacat
     5581 tttcgttaat ttgtacgctg acttcgccgt cgctaaagcg acctacagcg gcgtcgccga
     5641 gtgaagtgta caggcggttg gcaatacgtt gtgctagtac cggggtggcg ttaccagcaa
     5701 aaagcttcat atcaggcacg agaagaacct caggcatgcg tccattggtg gaaagaatct
     5761 gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg gtgtattaaa gagcgcgatg
     5821 caacgtctgg aacaaggtga cgttgtcacc gaaactcagc ttgcccggct taaagcatgg
     5881 ctctgtgcaa tgaggaaaga ttaacgcctt tcgccacaaa gccattgagc cattccgggg
     5941 cttgctctag cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag acacaggccc
     6001 ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc gcatcaacct
     6061 cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct gaattcacat tttagcaacg
     6121 tttctattga cctttttggc gtattgcgcg ggagttcagg atctttaaaa atcaccggag
     6181 tcggaatact tacaccaggg tgcgccacca gataccactt ctctggcgga tccaccggcg
     6241 ttagtatttc accaacgcct tcggcaaacg cggcatgccc ccgaacaaag acaggaacat
     6301 ctgcgcccag cgtcagcccc atttccgcca gctcatccat gcttagcccg cattgccaga
     6361 gatgatttaa tgccaccagg accgtcgcgg cattggatga accaccgccg agaccgccgc
     6421 ccatcggcaa acgcttgtca atgctgatat tcgcaccgct tcccgtcgga agacgcccgc
     6481 tgtctgccgc agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta tcttcatgtt
     6541 ccacgccttc aacgggcgtt aacagacgaa tatccccatc gtcacgaagc tcaatgctga
     6601 tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa ccatccgcac
     6661 gctgaccggt aatgtataaa aacagattaa gttttgccgg agagggccac tgtgtccgca
     6721 ttatttcact atccagttat ccatttttaa cttgatgcgt tgaccaccgt cggtgagttc
     6781 catattggct ggcatcgcag gttgcgtttt ggtgtcataa ccaccataaa caaccttcca
     6841 gtttttgcca ttctggctgt aggtaatttc gctcaggcgg tactggtcgt ccagtttgta
     6901 gtcggttgca tcacccggta aacctaaaat ccactggcgc aagctgttga gcggaattgg
     6961 cattccggtc aatttgccaa tcatctcttc ggcgtcatcg gcggtataac gctgaccttt
     7021 attgtcgact aactgcacgt tacccggttg agcattcagc tccagttccg tgctgcccaa
     7081 tgggttagtg agcagcagac ggtagcgatc ctggccggtt tgctgccaga aaaagcgggc
     7141 gtacactttt tgttggtcag aaatataagc gaacgcgccg cgagtctgat actgattaag
     7201 attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt tgccaggacc
     7261 tttgggcgtg gtaacggaac aggcagtgag cacaagagca gccagcggta gcaggcggat
     7321 aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct tgagatacgt tgcagttata
     7381 acccttaatg ctagcgttac cgtccgctat cgtctatgtt caagttgtct taattgccag
     7441 aatctaacgg ctttcggcaa ttactccaaa agggggcgct ctcttttatt gatcttacgc
     7501 atcctgtatg atgcaagcag actaacccta tcaacgttgg tattatttcc cgcagacatg
     7561 acccttttag cactcggtat caaccataaa acggcacctg tatcgctgcg agaacgtgta
     7621 tcgttttcgc cggataagct cgatcaggcg cttgacagcc tgcttgcgca gccgatggtg
     7681 cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac tttatcttag cgttgaagag
     7741 caggataacc tgcaagaggc gttaatccgc tggctttgcg attatcacaa tcttaatgaa
     7801 gaagatctgc gtaaaagcct ctactggcat caggataacg acgcggttag ccatttaatg
     7861 cgtgttgcca gcggcctgga ttcactggtt cttggggagc cgcagatcct cggtcaggtt
     7921 aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg ccagcgaact ggaacgcatg
     7981 ttccagaaat ctttctctgt agcgaaacgc gttcgcactg aaacagatat cggtgccagt
     8041 gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc agatctttga atcgctctct
     8101 acggtcacag tgttgctggt aggcgcgggc gaaaccatcg agctggtagc gcgtcatctg
     8161 cgcgaacata aagtacagaa gatgattatc gccaaccgca ctcgcgaacg tgcccaaatt
     8221 ctggcagatg aagttggcgc ggaagtgatt gccctgagtg agatcgacga acgtctgcgc
     8281 gaagccgata tcatcatcag ttccaccgcc agcccgttac cgattatcgg gaaaggcatg
     8341 gtggagcgcg cattaaaaag tcgtcgtaac caaccaatgc tgttggtgga tattgccgtt
     8401 ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg cttatcttta tagtgtggac
     8461 gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta aagccgcagc ggttgaggcg
     8521 gaaactattg tcgctcagga aaccagcgaa tttatggcgt ggctgcgagc acaaagcgcc
     8581 agcgaaacca ttcgcgagta tcgcagccag gcagagcacg ttcgcgatga gttaaccgcc
     8641 aaagcgttag cggcccttga gcagggcggc gacgcgcaag ccattatgca ggatctggca
     8701 tggaaactga ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca ggccgcccgt
     8761 gacggggata acgaacgcct gaatattctg cgcgacagcc tcgggctgga gtagcagtac
     8821 atcattttct ttttttacag ggtgcattta cgcctatgaa gccttctatc gttgccaaac
     8881 tggaagccct gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac gcgcaaacta
     8941 tcgccgacca ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta agtgatgttt
     9001 cgcgctgttt taccgactgg caacaggttc aggaagatat cgaaaccgca cagatgatgc
     9061 tcgacgatcc tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct aaagaaaaaa
     9121 gcgagcaact ggaacagcaa ttacaggttc tattactacc aaaagatcct gatgacgaac
     9181 gtaacgcctt cctcgaagtc cgtgccggaa ccggcggcga cgaagcagcg ctgtttgcag
     9241 gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg ccgctggcgg gtagaaatca
     9301 tgagcgccag cgagggtgaa catggtggtt ataaagagat catcgccaaa attagcggtg
     9361 atggtgtgta tggtcgtctg aaatttgaat ccggcggtca tcgcgtgcaa cgtgttcctg
     9421 ctacggaatc gcagggtcgt attcatactt ctgcttgtac cgttgcggta atgccagaac
     9481 tgcctgacgc agaactgccg gacatcaacc cagcagattt acgcattgat actttccgct
     9541 cgtcaggggc gggtggtcag cacgttaaca ccaccgattc ggcaattcgt attactcact
     9601 tgccgaccgg gattgttgtt gaatgtcagg acgaacgttc acaacataaa aacaaagcga
     9661 aggcgctttc agtgctcggt gctcgcatcc acgctgctga aatggcaaaa cgccaacagg
     9721 ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac cgtaaccgta
     9781 cttacaattt cccgcagggg cgcgttaccg atcaccgcat caacctgacg ctctaccgcc
     9841 tggatgaagt gatggaaggt aagctggata tgctgattga accgattatc caggaacatc
     9901 aggccgacca actggcggcg ttgtccgagc aggaataatg gaatatcaac actggttacg
     9961 tgaagcaata agccaacttc aggcgagcga aagcccgcgg cgtgatgctg aaatcctgct
    10021 ggagcatgtt accggcagag ggcgtacttt tattctcgcc tttggtgaaa cgcagctgac
    10081 tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg gtgaacccat
    10141 tgctcattta accggggtgc gagaattctg gtcgttgccg ttatttgttt cgccagcgac
    10201 cttaattccg cgcccggata cggagtgtct ggtggagcag gcactggcgc ggttgcctga
    10261 acaaccttgc cgtattctcg atctcgggac gggtaccggg gcgattgcgc ttgcgctggc
    10321 tagcgagcgc ccggactgtg aaattatcgc tgtagatcgt atgcctgatg ctgtctccct
    10381 ggcacaacgt aatgcccagc atctggcgat caaaaatatc cacattctgc aaagcgactg
    10441 gtttagcgcg ctagccgggc agcagtttgc gatgattgtc agcaatccgc cgtatattga
    10501 cgagcaggac ccacatcttc aacaaggcga tgtccgcttt gagccgctca ctgcgctggt
    10561 tgcggcagac agtggaatgg cagacatcgt gcatatcatc gaacagtcgc gtaacgcgct
    10621 ggtatccggc ggctttctgc ttctggaaca tggctggcag cagggcgaag cggtgcgaca
    10681 agcatttatc ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg gtgataacga
    10741 gcgcgtaacg ctcggccgct attatcaatg acaagttttt ctacactgct tagtgttcat
    10801 cttattagta tcgcgctttc tgttgggcta ttaaccttac gtttctggct acgttatcag
    10861 aagcatccac aggcatttgc tcgctggacg cgcattgtgc cgccggttgt cgatacggtg
    10921 ttattgttaa gcggcattgc gttgatggct aaagcgcaca tcctgccatt ttccgggcag
    10981 gcacagtggc tgactgaaaa gctgtttgga gttatcattt atatcgtttt gggttttatt
    11041 gcactcgatt atcgtcatat gcacagtcag caggcgcgca ttattgcctt cccgctggcg
    11101 ttggtggtgc tgtacatcat cattaaactc gccaccacaa aagtaccgtt actggggtaa
    11161 gtcatgagat cgttagctga tttcgaattt aataaagcgc cattgtgcga aggcatgatc
    11221 ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag atgtttacga cgaactggag
    11281 cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc tgcctttaga agagcagctg
    11341 gaaaaactga tcgcgctgtt ttacggcgac tggggattta aagcctcacg cggcgtttat
    11401 cgtctttccg atgcattatg gctggaccag gtgttaaaga atcgacaggg cagtgcggta
    11461 tcattaggtg cggttttatt atgggtcgcg aatcgtctcg atttgccgct gctgccggtg
    11521 attttcccta cgcagctgat attgcgcatt gaatgtccgg atggcgaaat ttggctgatt
    11581 aatcctttta acggtgaatc gttaagcgaa catatgctgg acgtatggtt aaagggaaat
    11641 atcagcccgt cggcggaact gttttatgaa aaccttgatg aagctgataa cattgaggta
    11701 atccgcaaat tgctggatac actcaaagcc atcgttgatg gaagaaaatc agatggagct
    11761 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11821 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11881 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11941 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    12001 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12061 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12121 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12181 tttgacaaag ccaaccgttc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12241 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12301 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12361 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12421 aagaaaccac agtttgtcag cccgggacag atgggtaata tcgttgataa attcaaagaa
    12481 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12541 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12601 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12661 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12721 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12781 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12841 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12901 cgttaagact tttttcatgg gtgccagata caaaaa
//
LOCUS       DADLSE010000103        12841 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLSE010000103 DADLSE010000000
VERSION     DADLSE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.103"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN692_002947"
     CDS             complement(221..2290)
                     /gene="glyS"
                     /locus_tag="JN692_002947"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7265210.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN692_002948"
     CDS             complement(2300..3211)
                     /gene="glyQ"
                     /locus_tag="JN692_002948"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7265211.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3306..3605)
                     /locus_tag="JN692_002949"
     CDS             complement(3306..3605)
                     /locus_tag="JN692_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265212.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3780..4775
                     /locus_tag="JN692_002950"
     CDS             3780..4775
                     /locus_tag="JN692_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7265213.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKSVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4817..5254)
                     /locus_tag="JN692_002951"
     CDS             complement(4817..5254)
                     /locus_tag="JN692_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7265214.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5300..5641)
                     /locus_tag="JN692_002952"
     CDS             complement(5300..5641)
                     /locus_tag="JN692_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265215.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIDVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN692_002953"
     CDS             complement(5810..7264)
                     /gene="xylB"
                     /locus_tag="JN692_002953"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7265216.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAMVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN692_002954"
     CDS             complement(7336..8658)
                     /gene="xylA"
                     /locus_tag="JN692_002954"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HAY7265217.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9024..10016
                     /gene="xylF"
                     /locus_tag="JN692_002955"
     CDS             9024..10016
                     /gene="xylF"
                     /locus_tag="JN692_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7265218.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10094..11635
                     /gene="xylG"
                     /locus_tag="JN692_002956"
                     /pseudo
     CDS             10094..11635
                     /gene="xylG"
                     /locus_tag="JN692_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
     gene            11613..12794
                     /gene="xylH"
                     /locus_tag="JN692_002957"
     CDS             11613..12794
                     /gene="xylH"
                     /locus_tag="JN692_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7265219.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMCRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
BASE COUNT         3238 a         3247 c         3099 g         3257 t
ORIGIN      
        1 tctgcctttc ggtgaatacc tgagacaaac agtctcaagc acccgtggct attctagctt
       61 aataagtttg tttcttctcc ttgatataat cctaaaaaaa tctcataaaa ttaatatatg
      121 agataatctt tattcagcag aagattatta aaggttgctg tattatttag cgataataaa
      181 aaaagcctgc cagatggcag gctttttaat aacggcgtta ttactgcaac agcgaaatat
      241 ccgcaacgcg caggaacagt tcgcgcagtt tctccagcat ggtcagacgg ttgagacgca
      301 attctttgtc atcaaccatg accatcactt tatcgaagaa agcatcaacc ggctcacgca
      361 gctctgccag ttcgaccagc gcatcctggt aacgaccttc cgcaaagtac ggctccagct
      421 tgtcacgtag caccacaacc tgcatcgcca gtttaatttc ttccggctct ttcagggtag
      481 aggcattcac gcgatcgctc agcacttcgt cagatttcgc caggatgtta gatacacgtt
      541 tgttcgccgc cgccagtgct gcagctgctt ccagggtacg gaagtgcgat accgctttca
      601 tgcgggcatc gaaatcagcc ggacgagtcg gacgacgcgc cagtaccgcc tggatggtgt
      661 caacggtata accttcgtcc tgataccagg cgcggaagcg accgagcata aagtcgataa
      721 catcatcaac tacgttggca ttagtcagct tatcgccata cagacgcacc gcctcttcgg
      781 tcagcgtttg cagatcaagg ttgaggttct tctcaacgat aattcgcagc acgccaagtg
      841 cggcacgacg cagcgcaaac gggtctttgt cgcctttcgg atgctgaccg ataccgaaga
      901 tacccgccag agtatccatc ttatcagcaa tcgccagcgc acaagctacc gggttggacg
      961 gcaggtcatc accggcaaag cgcggctgat actgctcatt cagcgcgacg gctacgtctt
     1021 ccgcttcgcc atcgtgacgc gcgtagtgca tccccataac gccttgggtg tcggtgaact
     1081 cgaagaccat gttggtcatc aggtcacact tagacagcag gcccgcacgg gttgcgtggt
     1141 taacgtcagc gccaatctgt tcggcgatcc agccagccag cgcctggatg cggtcagtct
     1201 tgtcacgcag cgtacccagc tgttgctgga acagcacggt ttgcaggcgc ggcaggttat
     1261 cttccagacg ttttttgcgg tcggtgttga agaagaactc ggcatccgcc agacgtggac
     1321 gaacgacttt ctcgttacca gagataattt gctgcggatc tttcgattcg atgttggcaa
     1381 cgaagataaa gttcggcagc agtttgccgt cgttcgcata caccgggaag tacttctggt
     1441 cacctttcat ggtgtaaacc aaggcttcag ccggcaccgc gaggaatttc tcttcgaatt
     1501 ttgcggtcag aacgaccggc cactccacca gcgaagccac ttcttccagc aggctttcgc
     1561 ttaagtcagc gttaccgcca atcttacgcg ctgcttcttc ggcatcggct ttaatcttcg
     1621 ctttacgttc ttcgtaatcg gcgatgactt tcccgcgctc acgcagaatt tccggatact
     1681 gatcggcgtt gtcgatggtg aattccggtt cgcccataaa gcggtggccg cgaatcacgc
     1741 gatcggactg aatgcccaga atggttgccg gaatgacttt gtcgcccagc agcagggtca
     1801 cggtgtgcac cggacgcacg aagtgcacgt cgcttgcacc ccagcgcatc agtttcggga
     1861 tcggcagttt cgccagcgaa gtcgcaacca tattcggcag cagtgcttcg gtgctttcgc
     1921 ccttcacatg ggcgcgatac agcagccatt cgcctttatc ggtagtcaga cgctcagcct
     1981 ggtcaacggt aataccacaa ccacgcgccc aaccttctgc cgctttgctc ggttttcctt
     2041 cagcgtcgaa cgcctgggca atcgccgggc cgcgtttttc gatttcacga tctggttgtg
     2101 cttccgccag gttagctact ttcagcgcca gacgacgcgg agcagcaaac cattgaacgg
     2161 tgccgtgtgc gaggcctgcg ttatccagct ccgcagtaaa gttcgcagca aaggactctg
     2221 ccaggctgcg cagtgctttt ggtggcagct cttcagtgcc gatttccacc agaaaagttt
     2281 tctcagacat agccgcctct tacttatctt tgttgcacat cgggaagccg agggcttcac
     2341 gggaagcgta atatgcttct gccactgctt tggtcagggt gcgaatgcgc agaatgtagc
     2401 gctgacgctc ggtgacggag atggctttac gcgcatccag caggttgaag ctgtgggcgg
     2461 ctttcagaat acgctcgtag gctggcagcg gcagcggatt ttccagcgcc agcagctgct
     2521 gcgcttcttt ctcgtactgc tcaaagcagg tgaacaggaa gtccacatcc gcgtattcga
     2581 agttgtaagt ggactgctcc acttcgttct gatggaacac gtcgccgtag gtggttttac
     2641 ccagcgggcc gtcgctccag accaggtcgt aaacgctgtc tacgccctga atgtacatgg
     2701 ccagacgttc cagaccgtag gtgatctcgc cggtaaccgg tttacactcc agaccaccaa
     2761 cctgctggaa gtaagtgaac tgcgtcactt ccatgccgtt tagccacact tcccagccca
     2821 gtccccaggc ccccagagtc gggttttccc agttatcttc cacgaaacgg atgtcgtgaa
     2881 tagtcgggtc catgcccagc tctttcagag aaccgaggta cagctcctga atattgtccg
     2941 gcgatggctt aatgaccacc tggaactgat agtagtgctg taaacggttg gggttttcgc
     3001 cgtagcgacc atcggtcggg cgacgagaag gctgaacata agcagccgcc atcggttctg
     3061 gccccagcgc gcgcagacag gtcattgggt gagaggttcc cgcgccgact tccatgtcca
     3121 atggttgaac aatggtgcag ccctggcgag cccagtaatc ctgtaaggtc aggatcaagc
     3181 cctggaaggt cctggtatca aacttttgca tattatttcg tgctggatac gtgtggattt
     3241 aaaggaaggg atcagtatac ccgctggatg gaagatatac agtacgaaac gggaaaaagc
     3301 agggcttaac gcatggaaag atgcaaaaaa tggccatctg cgtcaaaaga acagacaaac
     3361 tgctctttac ggaaggtata accgcgcatt tcatagcttc cctggaactg ttcgaaggcg
     3421 gtgacgtcga ttttttgctc gccggtgtta taacgttttg ccgcctgatc cttgcataac
     3481 gcttccatat tcaacgaacg cagaggatca acctttaccc gttgtgcttt ctgcacgggt
     3541 gacggtgtag aacaacccac tagcaccata agcgccattg ccggaaagaa agcgttcatc
     3601 atcattaaat taccgtctgg tttgcaggtg agtcttatta tttatatcgg tatagtggct
     3661 gatatcttac gaatttcctt aagcctctat aggctgtagt gataaaactc agattattcc
     3721 agtgcgccaa ccaaatgatt tatcggtcag acttttttta accagataca gagacacaga
     3781 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
     3841 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
     3901 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
     3961 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
     4021 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
     4081 atatggagtt agcgctgaaa aacctgctgc aaaagtcagt gttttaccac ttgtggtttt
     4141 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
     4201 gaaaaatgtt gctggtgcta atggtggtga ttggcattat cgctaaccca aacacagtgc
     4261 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
     4321 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
     4381 aagcactgtc gtgggtgagc gccgcgctgt ttgcgactgg ggtttttatt atctcacgcg
     4441 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
     4501 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
     4561 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
     4621 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
     4681 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
     4741 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     4801 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     4861 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     4921 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     4981 ccatcagtag tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     5041 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     5101 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     5161 atagccctaa caggtaggta acgataccac caacgagagc tatccatgac acaatactaa
     5221 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     5281 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     5341 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacat
     5401 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     5461 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     5521 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     5581 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     5641 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     5701 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat taattagtga
     5761 gatatataga tgtgaattat cccccacccg gtcaggcagg ggataacgtt tacgccatta
     5821 atggcagaag ttgctgatag aggcgacgga acgtttcacg tcgtggctga taagcggcat
     5881 aacgctgcgc atctggtaga tgcgactgtt ctaacggtag ctgcggcaac aactcaatga
     5941 gcgatttctc tggattcgcc gcaatctgcg ccagccttgc tgcgcccagt gctggcccca
     6001 catccccccc cgtacggtaa tcgagctgct gaccgctgat atccgccagc atctgacgcc
     6061 agtactcact acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg
     6121 catgcacgac atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg
     6181 ccagttcatt ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat
     6241 tattgtgtgg tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcggcacttt
     6301 catcagcctg ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg
     6361 cgacccaatc cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg
     6421 gtagcgcatg gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga
     6481 cagcaaaata gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc
     6541 ccacaccaac tgcaccagct gcattgtcgc caccgcctgc gacaactggc accatcgcca
     6601 taccccacgc tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata
     6661 atgcgggcat ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac
     6721 gctttgcgac atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc
     6781 ccgtcatacg cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata
     6841 tctccggctc atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca
     6901 ggttgccggt aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg
     6961 cacagcgccc gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta
     7021 aggttgctcc atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct
     7081 gatcgcccag agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt
     7141 ccgaccaaag tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa gcaaccacct
     7201 caccctgctc gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat
     7261 acatatcgat cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga
     7321 ctgcacagtt agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc
     7381 ctggcgacca ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc
     7441 tgccagtgac atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata
     7501 acgctgcgcg atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag
     7561 cgccagtgcc atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt
     7621 actttgacga cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt
     7681 gagaatttca tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc
     7741 ccagcccagt tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat
     7801 ggcggtggct atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat
     7861 gttcagttta atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc
     7921 atcgtaatca tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtacc
     7981 ctggaagccg attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc
     8041 acgctcctga cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag
     8101 gacatagttt tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc
     8161 cgcccagctg aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt
     8221 tgtaaagcag ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc
     8281 caggacatca accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg
     8341 ggaaacatcc acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa
     8401 tgcgacatct gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg
     8461 attaaacgcc cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca
     8521 ggcggcaaaa cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta
     8581 gtgacggaat gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg
     8641 gtcaaaatag gcttgcatat tgaactccat aatcaggtaa taccgcgggt gatggatgat
     8701 gtcgtaatat tgggcactcc ctttcagttg ctcaattatg ttatttcaca ctgctattga
     8761 gataattcac aagtgtgcgc tcgctcgcaa aataaaatgg gatgatgaaa ctgggtaatt
     8821 cctcgaagat aaaaatgcaa taagtacaat tgcgcaacaa aagtaagatc tcggtcataa
     8881 atcaagaaat aaaccaaaaa tcgtaatcga aagataaaaa tctgtaattg ttttcccctg
     8941 tttagttgct aaaaattggt tacgtttatc gcggtgattg ttacttatta aagctgtcct
     9001 ctaactacag aaggccctac accatgaaaa taaagaacat tctactcacc ctttgtacct
     9061 cacttctgct taccaacgtt gcggcacacg ccaaagaagt caaaataggt atggcgattg
     9121 atgatctccg tcttgaacgc tggcaaaaag atcgagatat ttttgtgaaa aaggcagaat
     9181 ctctcggtgc gaaagtattt gtacagtctg caaatggcaa tgaagaaaca caaatgtcgc
     9241 agattgaaaa catgattaac cggggtgtcg atgttcttgt cattattccg tataacggtc
     9301 aggtattaag taacgttgta aaagaagcca aacaagaagg tattaaagta ttagcttacg
     9361 accgtatgat taacgatgcg gatatcgatt tttatatttc tttcgataac gaaaaagtcg
     9421 gtgaactgca ggcaaaagcc ttggtcgata ttgttccgca aggtaattac ttcctgatgg
     9481 gcggctcgcc ggtagataac aacgccaagc tgttccgcgc cggacaaatg aaagtgttaa
     9541 aaccttacgt tgattctgga aaaattaaag tcgttggtga ccaatgggtt gatggctggt
     9601 taccggaaaa cgcattgaaa attatggaaa acgcgctaac cgccaataat aacaaaattg
     9661 atgctgtagt tgcctcaaac gatgccaccg caggtggggc aattcaggca ttaagcgcgc
     9721 aaggtttatc agggaaagta gcaatttccg gccaggatgc ggatctcgca ggtattaaac
     9781 gtattgctgc cggtacgcaa actatgacgg tgtataaacc tattacattg ctggcaaata
     9841 ctgccgcaga aattgccgtt gaattgggca atggtcagga gccaaaagca gatacctcac
     9901 tgaataatgg cctgaaagat gtcccctccc gcctcctgac accgatcgat gtgaataaaa
     9961 acaacatcaa agatacggta attaaagacg gattccacaa agagagcgag ctgtaagcgt
    10021 tacgccccgg cgcggagcgg gggcgtgatt tctctccatg ccgcgtgaat gaattggctt
    10081 aggtggagtc gttatgcctt atctacttga aatgaagaac attaccaaaa ccttcggcag
    10141 tgtgaaggcg attgataacg tcagcttgcg gttgaatgct ggcgaaatcg tctcactctg
    10201 tggggaaaat gggtctggta aatcaacgct gatgaaagtg ctgtgtggta tttatcccca
    10261 tggctcctac gaaggcgaaa ttatttttgc gggagaagag attcaggcga gtcacatccg
    10321 cgataccgaa cgcaaaggta tcgccatcat tcaccaggaa ttggccctgg tgaaagaatt
    10381 gaccgtgctg gaaaatatct tcctgggtaa cgaaataacc cacaatggca ttatggatta
    10441 tgacctgatg acgctacgct atcagaagct gctcgcacag gtcagtttat ccatttcacc
    10501 tgatacccgc gttggcgatt tagggcttgg gcaacaacaa ctggttgaaa ttgccaaggc
    10561 acttaataaa caggtgcgct tgttaattct cgatgaaccg acagcctcat taactgagca
    10621 ggaaacgtcg gttttactgg atattattcg cgatctacaa cagcacggta tcgcctgtat
    10681 ttatatttcg cacaaactca acgaagtcaa agcgatttcc gatacgattt gcgttattcg
    10741 cgacggacag cacattggta cgcgtgatgc tgccggaatg agtgaagacg atattatcac
    10801 catgatggtc gggcgagagt taaccgcgct ttaccctaat gaaccacata ccaccggaga
    10861 tgaaatatta cgtattgaac atctgacggc atggcatccg gtcaatcgtc atattaaacg
    10921 agttaatgat gtctcgtttt ccctgaaacg tggcgaaata ctgggtattg ccggactcgt
    10981 tggtgccgga cgtaccgaga ccattcagtg cctgtttggc gtgtggcccg gacaatggga
    11041 aggaaaaatt tatattgatg gcaaacaggt agatattcgt aactgtcagc aagccatcgc
    11101 ctaggggatt gcgatggtac ccgaagacag aaagcgcgac ggcatcgttc cggtaatggc
    11161 ggttggtaaa aatattaccc tcgccgcact caataaattt accggtggca ttagccagct
    11221 tgatgacgcg gcagagcaaa aatgtattct ggaatcaatt cagcaactca aagttaaaac
    11281 gtcgtccccc gaccttgcta ttggacgttt gagcggcggc aatcagcaaa aagcgatcct
    11341 cgcccgctgt ctgttactta acccgcgcat cctcattctt gatgaaccta ccaggggtat
    11401 tgatattggc gcgaaatatg agatctacaa attaattaac caactcgtcc agcagggtat
    11461 tgccgttatt gtcatctctt ccgaattacc tgaagtgctc ggccttagcg atcgtgtact
    11521 ggtgatgcat gaagggaaac taaaagccaa cctgataaat cataacctga ctcaggagca
    11581 ggtgatggaa gccgcattga ggagcgaaca tcatgtcgaa aagcaatccg tctgaagtga
    11641 aattggccgt accgacatcc ggtggctttt ctgggctgaa atcactgaat ttgcaggtct
    11701 tcgtgatgat tgcagctatc atcgcaatca tgctgttctt tacctggacc accgatggtg
    11761 cctacttaag cgcccgtaac gtctccaacc tgttacgcca gaccgcgatt accggcatcc
    11821 tcgcggtagg aatggtgttc gtcataattt ctgctgaaat cgacctttcc gtcggctcaa
    11881 tgatggggct attaggtggc gtcgcggcga tttgtgacgt ctggttaggc tggcctttgc
    11941 cacttaccat cattgtgacg ttggttctgg gactgcttct cggtgcctgg aacggatggt
    12001 gggtcgcgta ccgcaaagtc ccttcattta ttgtcaccct cgcgggcatg ttggcatttc
    12061 gcggcatact cattggcatc accaacggca cgactgtatc ccccaccagc gccgcgatgt
    12121 cacaaattgg gcaaagctat ctccccgcca gtaccggctt catcattggc gcgcttggct
    12181 taatggcttt tgttggttgg caatggcgcg gaagaatgtg ccgtcaggct ttgggtttac
    12241 agtctccggc ctctaccgca gtagtcggtc gccaggcttt aaccgctatc atcgtattag
    12301 gcgcaatctg gctgttgaat gattaccgtg gcgttcccac tcctgttctg ctgctgacgt
    12361 tgctgttact cggcggaatg tttatggcaa cgcggacggc atttggacga cgcatttatg
    12421 ccatcggcgg caatctggaa gcagcacgtc tctccgggat taacgttgaa cgcaccaaac
    12481 ttgccgtgtt cgcgattaac ggattaatgg tagccatcgc cggattaatc cttagttctc
    12541 gacttggcgc tggttcacct tctgcgggaa atatcgccga actggacgca attgcagcat
    12601 gcgtgattgg cggcaccagc ctggctggcg gtgtgggaag cgttgccgga gcagtaatgg
    12661 gggcatttat catggcttca ctggataacg gcatgagtat gatggatgta ccgaccttct
    12721 ggcagtatat cgttaaaggt gcgattctgt tgctggcagt atggatggac tccgcaacca
    12781 aacgccgttc ttgattttga taaaaatttt ctcaaagccg gttacgtatt accggttttg
    12841 a
//
LOCUS       DADLSE010000104        12806 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLSE010000104 DADLSE010000000
VERSION     DADLSE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.104"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(286..492)
                     /locus_tag="JN692_002958"
     CDS             complement(286..492)
                     /locus_tag="JN692_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7265220.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            805..882
                     /gene="kdpF"
                     /locus_tag="JN692_002959"
     CDS             805..882
                     /gene="kdpF"
                     /locus_tag="JN692_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009651669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7265221.1"
                     /translation="MSAGVLLVFLLLGYLVYALINAEAF"
     gene            882..2531
                     /gene="kdpA"
                     /locus_tag="JN692_002960"
     CDS             882..2531
                     /gene="kdpA"
                     /locus_tag="JN692_002960"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HAY7265222.1"
                     /translation="MAAQGFLLIATFLLVLMVLARLINDIPLPGTAGVERILFRLPGV
                     SDHEMNWKQYLCAILGLNMLGLAELFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFV
                     TNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSMSTLGNAWV
                     DLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQEAIK
                     MLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGDRRQGRMLL
                     WAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSLFAVVTTAA
                     SCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGR
                     TPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLY
                     AVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQPAS
                     SGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2554..4601
                     /gene="kdpB"
                     /locus_tag="JN692_002961"
                     /pseudo
     CDS             2554..4601
                     /gene="kdpB"
                     /locus_tag="JN692_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4610..5182
                     /locus_tag="JN692_002962"
     CDS             4610..5182
                     /locus_tag="JN692_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HAY7265223.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5175..7859
                     /gene="kdbD"
                     /locus_tag="JN692_002963"
     CDS             5175..7859
                     /gene="kdbD"
                     /locus_tag="JN692_002963"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HAY7265224.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFSACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVDNALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7856..8533
                     /gene="kdpE"
                     /locus_tag="JN692_002964"
     CDS             7856..8533
                     /gene="kdpE"
                     /locus_tag="JN692_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7265225.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8808..8912
                     /locus_tag="JN692_002965"
     CDS             8808..8912
                     /locus_tag="JN692_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7265226.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9223..11410
                     /gene="speF"
                     /locus_tag="JN692_002966"
                     /pseudo
     CDS             9223..11410
                     /gene="speF"
                     /locus_tag="JN692_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11407..12726
                     /gene="potE"
                     /locus_tag="JN692_002967"
     CDS             11407..12726
                     /gene="potE"
                     /locus_tag="JN692_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7265227.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSTTLYVDSWNPHHAPFFSTVGSSIAMTLWAFLGLESACANTDVVEN
                     SERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
BASE COUNT         2872 a         3187 c         3524 g         3223 t
ORIGIN      
        1 aagggatgac ggctttctca ccttaccctg aagtttcacg aaagtgtgta aggaagtatg
       61 caatctaact ggagatttct gatgatctag tcgcttagag cgacaatgaa ggtgaccgca
      121 tcctgggtag ccctatgaaa ggatactatt gaaacgtgat gctaaaacag tgatgaaatg
      181 aataatacga tggaaacgtc ttccatgtta attatcatgg aagacgttga tgttgaacac
      241 ctaaaccagc cccctgatac gaggctggct taagcgcgca ggcaatcagt aaaaatcacc
      301 ggttgccttt tcggcctgat ccatccacac cggtttatcg ctggttttcg accagacgcg
      361 gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa agcatgaaag gcagcgccag
      421 aacgcccgct gcaaccgcat atgtacggcg taaaaagata agccatgcag gatattctct
      481 gtagagttcc atacttttct cccccttttg tatctacccg gtgaatagca ccggaaaaat
      541 gaatttgttt atctgatgaa aatagtaccg ccttttgtgt aattttacta ctcatccgac
      601 cacttatttt tgcttattga tggtttattt acattcacac tgtaattaaa ttacacaaaa
      661 gttaaattaa tactaaccat cagttaaatc atggcctttg ccatttttat acttttttta
      721 caccccgtcc gcagattttt gcgaaatctt tgcagccaga attctactct tccggtatca
      781 cttttaggcc actggaggtg cactgtgagt gcaggcgtgt tgctggtgtt tttattactg
      841 ggttatttgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct
      901 tactgattgc cacgttttta ctggtattaa tggtgctggc gcggctgatt aatgacattc
      961 ctcttccagg tacagcgggc gttgaacgta tactctttcg tttacctggc gtctctgacc
     1021 atgagatgaa ctggaagcaa tatctttgtg ccattctcgg cctgaacatg ctggggctgg
     1081 cggagctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca cagcagttgc
     1141 cggggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg aataccaact
     1201 ggcaatctta tagcggtgaa accacgttga gctatttcag ccagatggca ggcttaacgg
     1261 tacaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccttc atccgtgcgt
     1321 ttactcgcca gagcatgagc acactcggga atgcctgggt cgatctgcta cgcatcacgt
     1381 tatgggtgct agtccctgtg gcgttgttga tcgcactgtt ttttattcaa caaggtgcgc
     1441 tgcaaaactt tctgccctat caggcagtga ataccgttga aggagcgcaa cagctgttac
     1501 ccatgggacc agtagcttct caggaagcga tcaagatgct cggtactaac ggcggtggct
     1561 tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc aacttcgtgc
     1621 agatgctggc gatcttcttg atcccaacag cgctgtgctt tgcctttggt gaagtgacgg
     1681 gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt gtcatctgcg
     1741 taggcgtggt tatgtgggca gaagttcagg gtaatcctca tctgctggcg ctcggcgcag
     1801 acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc agtagcctgt
     1861 ttgcggtcgt gactacggcg gcgtcctgtg gcgcggtgat tgcgatgcat gattcgttta
     1921 ccgctctcgg cggcatggtg ccgatgtggc taatgcaaat tggtgaagtg gtgttcggcg
     1981 gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg tttattgccg
     2041 ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta cgcgagatga
     2101 aactgactgc actggcgatt ctggttactc caacactggt actgatgggc gcggcgttgg
     2161 cgatgatgac cgacgccgga cgtagcgcca tgcttaaccc tggcccgcat ggttttagcg
     2221 aagtgctgta cgccgtgtcg tccgccgcta acaacaacgg cagcgccttt gccggattaa
     2281 gcgccaactc tccgttctgg aactgtttac tggcgttctg catgtttgtc ggtcgcttcg
     2341 gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag agccaacccg
     2401 ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta atcggcaccg
     2461 tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtccg gtggcggaat
     2521 atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact ggcgctattc
     2581 gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa cccgcaggcg
     2641 caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac cacctgtatt
     2701 agcatcgcga tggcaagcgg tgcgatgcat ggcaatgcgc tgtttagcgc ggcaattagc
     2761 ggctggctgt gggtcaccgt actgttcgct aatttcgccg aggcgctggc agaaggccgc
     2821 agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc ccgcaagctg
     2881 cgtgagccga aatatggcgc tgcggcggac aaagttcctg ccgatcaact gcgtaaaggt
     2941 gacatcgtgc tggtagaagc tggcgatatt atcccctgcg atggtgaagt cattgaaggc
     3001 ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgat ccgtgaatcc
     3061 ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg gctggtgatt
     3121 gagtgtagcg ttaaccccgg tgagacattt ctggatcgga tgatcgcgat ggtggaaggc
     3181 gcacagcgac gcaaaacacc gaacgagatt gccctgacta tcctgctgat tgctctgacc
     3241 atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg cggtaatgcc
     3301 gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac tattggtggc
     3361 ctgttgtcag cgatcggcgt cgccgggatg agtcggatgc tgggcgcgaa tgtgattgcc
     3421 accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact ggataaaacc
     3481 ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccccgcgca gggcgtggat
     3541 gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac gccggaaggt
     3601 cgcagtattg tgatcctcgc caagcaacgt tttaacctgc gcgagcgcga tgtgcagtcg
     3661 ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat caacatcgac
     3721 aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc gccatattga ggctaacggc
     3781 ggtcacttcc ctgccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca aggagccacg
     3841 ccgctggtgg tggtggaagg ttctcgtgtg ctgggcgtta ttgcgctgaa agatatcgtc
     3901 aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaat gggcattaaa acggtgatga
     3961 ttaccggcga taaccgtctg actgccgccg cgatagctgc ggaagcgggt gtcgatgatt
     4021 ttctcgccga agcgacaccg gaggccaagc tggcattgat ccgtcagtat caggcggaag
     4081 gtcgtttggt agcgatgacc ggcgacggca ccaacgatgc tccggcgctg gcgcaggcag
     4141 atgtcgcggt ggcgatgaac tccggcaccc aggcggcgaa agaggcgggc aatatggtgg
     4201 atctcgactc taacccgacc aagttgatcg aggtggtgca cattggcaaa cagatgctga
     4261 tgacccgtgg ctcgctgacc accttcagca ttgccaacga tgtggcgaaa tacttcgcca
     4321 ttattccggc ggcgttcgcg gcaacatatc cgcagttaaa tgcgctgaac atcatgcgcc
     4381 tgcattcgcc cgactccgcc attctcagtg cggtcatttt caacgccttg attatcgtct
     4441 ttttgattcc tctggcgtta aaaggcgtga gttataaacc gcttaccgct tctgccatgt
     4501 tgcgccgtaa cttatggatt tacggtctgg gtgggctgct ggtgccgttt attggtatca
     4561 aagtcattga tttactgcta accgtttgta gtctggtgtg aggtttacca tgagtggatt
     4621 acgtccggca ttatcgacat ttctctttct gttattgatt actggcggcg tttacccgct
     4681 gctgaccacc gcactggggc aatggtggta tccctggcag gccaacggtt cgttgattcg
     4741 tgaaggtgat acggtgcgcg gttcggcatt aatcgggcaa aattttaccg gcaacggcta
     4801 ttttcatggt cgcccgtcgg caacagcaga aatgccctat aatccacagg cttctggcgg
     4861 gagcaatctg gcagtcagta acccggagct ggataaacag atagccgcac gcgttgccgc
     4921 attacgggcc gctaacccgg acgccagcac gaacgttccg gttgaactgg tgacggcgtc
     4981 ggcaagcgga ctggacaata acatcacccc gcaagcggcg gcctggcaga tcccacgcat
     5041 cgcaaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca
     5101 acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc
     5161 actggataag cttgatgaat aacgaaccct tacgtcccga ccccgaccgt ctgctggaac
     5221 aaactgccgc gccgcatcgg gggaagctga aagttttctt cagtgcctgt gcaggcgtcg
     5281 ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg ctggatattg
     5341 tggttggtgt ggtagaaacc catgggcgga aagataccgc cgccatgctt gaagggctgg
     5401 ctgttctgcc tctgaaacgc caggcgtacc gtgggcggca tatcagcgag tttgatctcg
     5461 atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg cacagtaatg
     5521 cgccaggttc tcgtcatcct aaacgctggc aggatatcga agaactgctg gaagctggca
     5581 ttgatgtttt cacaaccgtc aacgttcagc atctggaaag tctgaatgat gtggtcagcg
     5641 gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg
     5701 acgtggtgct ggtggacttg cccccggacg atctgcgcca gcggctgaaa gaaggcaaag
     5761 tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga
     5821 tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg
     5881 cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg atccttttat
     5941 gcatcggcca taacaccggc agcgaaaaac tggtccgcgc ggcggcgcgg ctggcgtcac
     6001 ggctggggag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcat cgcttaccgg
     6061 agaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga
     6121 cggcaacact ttctgatcca gcggaagaga aagcggtagt gcgttatgcc cgtgaacata
     6181 atctcggcaa gattattctc ggtcgcccag cctcgcgccg ctggtggcgt cgggaaacat
     6241 ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc gcgcttgatg
     6301 aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcatttaaa gacaagtggc
     6361 gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc accttaattg
     6421 ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat ctgcttggcg
     6481 tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgccacc gtcattaatg
     6541 tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc
     6601 aatatctgct gaccttcgcg gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg
     6661 ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca cggcacttat
     6721 atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc gccaccagcg
     6781 aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc gatgacaacg
     6841 gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat gccatcgcgc
     6901 agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac
     6961 cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg gtggtggagc
     7021 cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag acgtttacgc
     7081 tgttagtcga caatgccctt gagcggctga cgctaaccgc cagcgaagaa caggcgcgga
     7141 tggcaagcga acgtgaacag atccgcaacg ccctgctggc agcgctttcg catgatttac
     7201 gcacgccgct tacggtgctg tttggtcagg cagaaatctt aacgctcgat ctggcaagcg
     7261 aaggatcacc ccacgcccgc caggccagcg agattcgtca gcatgtgctg aacactaccc
     7321 gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt aatttgaaga
     7381 aagagtggtt aacgctggaa gaagtagtcg gcagcgcgct gcaaatgctg gaaccgggtt
     7441 tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac gttgacgggc
     7501 cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc
     7561 aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg gatgtctggg
     7621 ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag tttgctcgcg
     7681 ggaataaaga gtcggcggtg ccgggtgtcg ggcttgggtt ggcaatttgt cgggcgattg
     7741 tggatgtaca cggcggcacc attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc
     7801 gtgttacact tccccagcaa accgctcctg aacttgaaga ctttcatgag gatatgtgac
     7861 aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca cggcgctgga
     7921 gggcgacggg atgcgcgtct ttgaggccga aactctgcaa cgcggcttgc tggaagcggc
     7981 aacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg atgggattga
     8041 gtttatccgc gacctgcgcc agtggagccc ggtgccggtg attgtgcttt ccgcacgcag
     8101 cgaagagagc gacaagatcg ccgcgctgga tgccggagcg gatgattatc tgagtaagcc
     8161 gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc actccgccac
     8221 cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt
     8281 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt
     8341 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     8401 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     8461 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg
     8521 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     8581 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8641 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8701 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8761 gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata
     8821 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttgcc catcgtaata
     8881 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     8941 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     9001 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     9061 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     9121 tttatttatc agcggtggag ctttgctttt tttccggcgt gatcgatttc tcctttgaga
     9181 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     9241 gccgttaacc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     9301 gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
     9361 aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
     9421 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
     9481 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa
     9541 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
     9601 gactgcccgg gccatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc
     9661 gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg
     9721 ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     9781 tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     9841 ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct
     9901 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt
     9961 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
    10021 gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct
    10081 gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
    10141 attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt
    10201 atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt
    10261 attctggtta cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc
    10321 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
    10381 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
    10441 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
    10501 aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg tccgttcgta
    10561 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
    10621 ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag
    10681 aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
    10741 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgtagt
    10801 gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag aagatatggc
    10861 caaacttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg agtccgacgc
    10921 gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct acgccggtta
    10981 taccctgcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca acgtgaaaca
    11041 actgcaaaaa gagatgttcc gcaaagcgca cttcccacac gtcagcatga atccgcaaga
    11101 agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgctgg atgccgaagg
    11161 tcgtatcgct gccgaagggg cgcttcctta tcctccgggt gtgctgtgtg ttgttccggg
    11221 tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag ggatcaacct
    11281 gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg atggtcgtaa
    11341 gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt tgaaagggga
    11401 aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac catactgacg
    11461 atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc cgaagttggg
    11521 acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact ggcatgggca
    11581 ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta tgccgagtat
    11641 gctttcggta aatccggtaa ctttatggcg aactatacct acggcgtctc actgctgatt
    11701 gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct cggcgcgagt
    11761 ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg taccgtggct
    11821 aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttaccgtgtg gggggtcatt
    11881 attccggtgg taggcctgtg cattatcggc tggttctggt ttagcacgac gctttacgtt
    11941 gattcctgga atccgcatca tgcaccgttc ttcagtacag taggttcttc catcgctatg
    12001 acgctgtggg cttttcttgg tctggagtct gcgtgtgcga atactgatgt agtggaaaac
    12061 tcggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc ggtgatttat
    12121 atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc aaattcaacg
    12181 gcaccatttg gcctggcctt cgcacagatg ttcacaccag aagtgggtaa agtcattatg
    12241 gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac cattgcccag
    12301 gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa
    12361 gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg gttggcgctg
    12421 atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct ggccgtggtg
    12481 accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct
    12541 aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg cgcgatgtat
    12601 agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc catcgtgacc
    12661 ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa aaataaacac
    12721 ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgacct aatgccagtc agttaagcaa
    12781 ctgactggct ctttttcggg gctgtg
//
LOCUS       DADLSE010000105        12786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLSE010000105 DADLSE010000000
VERSION     DADLSE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.105"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN692_002968"
     CDS             complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN692_002968"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7265228.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN692_002969"
     CDS             complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN692_002969"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7265229.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(778..1512)
                     /locus_tag="JN692_002970"
     CDS             complement(778..1512)
                     /locus_tag="JN692_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7265230.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN692_002971"
     CDS             complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN692_002971"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7265231.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLD
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3638..4615
                     /gene="epmA"
                     /locus_tag="JN692_002972"
     CDS             3638..4615
                     /gene="epmA"
                     /locus_tag="JN692_002972"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7265232.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            4834..6336
                     /gene="yjeM"
                     /locus_tag="JN692_002973"
     CDS             4834..6336
                     /gene="yjeM"
                     /locus_tag="JN692_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7265233.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMLSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN692_002974"
     CDS             complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN692_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7265234.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPML
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYFLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICCALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN692_002975"
     CDS             complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN692_002975"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7265235.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLACNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN692_002976"
     CDS             complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN692_002976"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7265236.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            12032..12577
                     /gene="orn"
                     /locus_tag="JN692_002977"
     CDS             12032..12577
                     /gene="orn"
                     /locus_tag="JN692_002977"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7265237.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         2907 a         3328 c         3394 g         3157 t
ORIGIN      
        1 gttagattgt aacgacacca atcagcgtga caactgtcag gatagcagcc agaccgtaga
       61 aaacccattt gcccgcaggc acgtggattt tcagatcgtg catcgcgtgg tgcatacggt
      121 gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga ccaatgaagc
      181 tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac agccccagtg
      241 gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc cacataccac
      301 cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt ggattaatca
      361 tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc aaccacagtt
      421 accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt tacaatgata
      481 ttggccgctt tcggtgccag ttcaaaccag gttttggtgt gcagcagagc tgccgtcaga
      541 gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa tcccgcccag
      601 gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct gaaccacaca
      661 gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa ttttttccac
      721 caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt gcactcctta
      781 gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc cctgctgaat
      841 ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca cgaaagtaca
      901 gctccataca ccgttctggc tgttcaactg cgccatacgc tccttcttac cgtggtcgcg
      961 gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga actctgggtt
     1021 caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac cggagaactg
     1081 gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg cggtgcggga
     1141 gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg tcatatcgac
     1201 caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac cgtcggtgta
     1261 atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca tgccgcagga
     1321 accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca ggttgtcttt
     1381 gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt catagaatgc
     1441 gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt tcaggttttt
     1501 catctcagcc attcgccttc tccttcttat tggctgcttc cgccttatcg gctgcatccg
     1561 cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg tcgctgtact
     1621 ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgaga aagttgacgt
     1681 cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac tctttacgtg
     1741 ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggcccagt tcaatggtgt
     1801 agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc ttgaagcgtt
     1861 cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta cggtagatac
     1921 cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag ttttcgccgc
     1981 catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc gcttcaattg
     2041 ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca ccggccagac
     2101 ggccgaagac caccagttcc gccagggagt tggagcccag acggtttgca ccgtgcagac
     2161 caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca cagttctgat
     2221 cggtttcgat accgcccatg gtgtagtgtg cagtcggacg taccggaatc ggttctttaa
     2281 ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga cgttcatgca
     2341 gttttttctc gccgaggtga cgcaggtcga gataaaccac atcaccacgc ggcgtggaga
     2401 tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg cgtggaccca
     2461 gttccatata tttgtttttc ggctcgtcca gcggagtttc cgggcccatg ccgtaatctt
     2521 gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccacgg cagccttcgg
     2581 tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat tccatgtcac
     2641 gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg atgccgccgt
     2701 tggtgttgta acgataaacg cgacccgcac cgccggtagc cataacgacc gcgttagcac
     2761 ggatctgcac caacgtgcct tccatcatgt tcattgctac caggccgcga acatgaccat
     2821 catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc gggaattgca
     2881 gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg gcgaaccagg
     2941 tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc gggcgacggc
     3001 tccatgggca tccccacagt tccagttggg tcatttcggt tgggcagtgg tggacgaaat
     3061 aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg tgaaagtgat
     3121 attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca gcaacggtat
     3181 ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcatttgga tttgcctgcg
     3241 cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca agatcggctt
     3301 gaaaggtttg cacgacattc ctccagattg tttttatccc acagccccgt acttcagggt
     3361 aagtgcctga aagttacggt ctgcgaacgc tattccactg ctcctttata ggtacaacag
     3421 tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg cgcaagaggg
     3481 ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt gcttttttta
     3541 gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg cgttgcgagt
     3601 agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg catcctggca
     3661 gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg agatccgtcg
     3721 tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc aggcgacggt
     3781 aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc attcgcaggg
     3841 gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgcctgc tggttgctgg
     3901 ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg ggcgttatca
     3961 caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt accggttgat
     4021 gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag aaagcctttc
     4081 ttatcaacaa gctttcttgc gttatctgga aattgacccg ctctctgccg acaaaacgca
     4141 actgcgggaa gtcgcagcga aactggattt gagcaatgtt gctgataccg aagaagaccg
     4201 cgacacgttg ctacaattgc tgtttacctt tggcgtagag ccaaatattg gtaaagaaaa
     4261 accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa tcagtaccga
     4321 agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc tggcgaatgg
     4381 tttccatgaa ttaacggatg cccgtgagca gcaacaacgc tttgaacaag ataaccgtaa
     4441 gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg aagccttgaa
     4501 agtcggtatg cctgactgtt ccggcgtggc attaggtgtt gatcgtctgg tgatgttggc
     4561 gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg cataattctg
     4621 aaaaattact gaattaacag cgaagaatgg cgtgataagc gccattctgt agcatatgtt
     4681 ttcatttttc cgttaaaggc cacaatttaa gactggatat ttgatatcat ccaggtatca
     4741 attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc agtgggcacg
     4801 tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa aaagatgagt
     4861 ctgataggac tcatattgat gatctttact tccgtatttg gatttgccaa tagcccatcg
     4921 gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc attgttattc
     4981 tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa agaagaaggt
     5041 ggtatctatt cttggatgaa taatagtgtc ggaccacgtt ttgccttcat tggtacgttt
     5101 atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt ttgggtaccg
     5161 ttctcaacat tcctctatgg tagtgacatg acccagcact ggcgtattgc cggactggag
     5221 cctacgcagg tggttggtct gctggcagta gcatggatga ttctggtcac cgtcgttgct
     5281 tcaaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc agtaatgtgt
     5341 ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg tgggcatttc
     5401 gcgcaggata ttaatttcct tgcatcaccg aacccgggtt atcagtccgg tctggcaatg
     5461 ctatcgtttg tggtatttgc tatttttgcc tatggcggaa ttgaagcggt tggtggtctg
     5521 gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt tgccgctatt
     5581 gttatttcaa tcggttattc gctggcaata tttttatggg gcgtcagcac aaactggcag
     5641 caggtattaa gtaatggttc cgttaacctt ggcaatatta cctatgtgct gatgaagagc
     5701 ctcggggtga cgctgggtaa cgcactgcat ttgtcacctg aagcgtcatt gtcgctgggc
     5761 gtatggtttg cgcgtattac cggactttcg atgttcctcg cttataccgg tgcgttcttt
     5821 acgctttgct attcaccgct gaaagccatc atccagggga cgccgaaagc gttgtggccg
     5881 gaaccgatga cgcgcctgaa tgcgatgggg atgctgtcta tcgccatgtg gatgcagtgc
     5941 gggttggtta ctgtcttcat tctgctggtt tcgtttggtg gcggtaccgc atcggcgttc
     6001 tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttatct gttcctcgcg
     6061 ctggctttcc cgttttttaa agcacgtcag gatctcgaca gaccatttgt gattttcaaa
     6121 acgcgtatgt cggcaatgat tgcgacggtg gttgtcgttc tggtggtgac atttgcgaac
     6181 gtcttcacca ttattcagcc tgtggttgaa gccggagact gggacagcac attgtggatg
     6241 attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa ctattgcagt
     6301 cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct gcaactcatc
     6361 gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct ccagggccgg
     6421 tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat ttggcagctt
     6481 catgcattac aaacttcccg cctgacgacc tttgcccgca gacgtcagcg ttctccccgt
     6541 ttgtttaccg ttgaggcttt ccagacgcat ctggaagggc gggaatggca tatcgatacc
     6601 gtgggcgtgg aagcctgcca gaatcagctg gtggatctca tggcgtagcg gcatacggtg
     6661 acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccctgtt gcagatccac
     6721 caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg ctgcggtgag
     6781 caggatttcc gtcacttctt cactgttggc atcggcaggg gccggaatcg tcaacactac
     6841 gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa acgccttgtt
     6901 cggcacgatt atctctttac ggtcccagtc gctgatggtg gtggcgcggg tgttaatttt
     6961 tgtcacgcta ccagtaagat cgcggatcgt taccgtatcg ccaatgcgaa tcggtttttc
     7021 gaacaggatg atcaggccag agataaagtt ggcgaaaatt tcctgcaaac caaaaccgag
     7081 accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac caatcattga
     7141 gaagccgacc agcccgccaa tcagcatcag cagatatttt gtgatggtgg tgatggcgta
     7201 acccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca gcgcgggcag
     7261 gttgcgcacc agctgcgtgg tgatgataaa taccagaatg gcaatcagca ccgcaccgag
     7321 ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc acagcgaaat
     7381 attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga ccgaaagcag
     7441 ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga tggcatcgag
     7501 atcgacttcg ctttcatcga cttcaattgc tccttccggg ctactgtgat gatgcgcttc
     7561 ctcttcgcca cgcgcacgtt gcgctaacat ctctgcccgg cgatgcttcg cccggtcaaa
     7621 cgccagcctg cgacgctgga tcagcatcca gcggcggata acgtgataaa ccaccagtag
     7681 caggaaccag atggcaaccg aggtttcaag cctcgctaac agtgcctgcg ccgttgccag
     7741 ataacccacc gccgacgcca gaatggcaac cagtggtgcg ccaatcatca tgttccacag
     7801 catatggttg gtaatgttgt cgccgctgcc ttctttgttg agatacaacg gaataccggc
     7861 ttttttcagg cttagggtga ccaccgccag cgcacagcaa atgaggataa agcaaagccg
     7921 tcccagcgaa ccggaaaatt cgcggtcgtc gaggttatcg aacatcatca gcgccataat
     7981 cagcccgatg ctcatcagga agtagcgcat cccacgggaa acacgttcgc gcggccagcc
     8041 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggtg gcgcaaatca tcactaccca
     8101 cagcatcggc acggtggccg ttacaccatc gccaatcgcg accgccagcg gataaggcca
     8161 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggtaacggtg acgcgacgag
     8221 aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga tcctgagtca ctttgccgac
     8281 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
     8341 gaccagaatc aacgcgccaa acagcggcaa aattgtctct ttgctggtca gcatcatcac
     8401 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
     8461 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
     8521 gtgcgttgct tcgttcacct ctttcagcgc atcctccagt tgcccgttgg agactttcag
     8581 cttggtcagt tccaatagta gcgtgtcgcc accctgcaac aatgagttca gcaactcacg
     8641 ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg gcggtcagcg gctgaccgtc
     8701 ggcctgatgg atttgccgta gcagcggctg tttattaagt aaatcctcat aacgtaaccg
     8761 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggttttggca tttctggtag
     8821 ccgtgccacc tgcgcccgca gcgcttcgcc gagcaggttg gacgatccca gccattgcga
     8881 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
     8941 ctgttgcgag gcaacgagat ccatccgctg cgcctgttga ttcagcgctg ccgatagttc
     9001 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgccag
     9061 taattcggta ctttccagcg cccgctccgc ctcaagctga cgttggctat tcaactgatt
     9121 acgtaaggcc tgcaaatacg catccagttg ctggctctct ttttccgcca gctctgagcg
     9181 taagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
     9241 gagcgcctta agacgtgcag agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
     9301 agtattgccg gtaagcgttc ccaggcggcg ctcgatctca tttaactgac ggcgggcgtc
     9361 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
     9421 ttgctgggcc tgacggcttt tatccagcaa ctggctgctg acctggagaa tttcctgatt
     9481 cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
     9541 ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg ataacttgct gatattgttt
     9601 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagcgcctc
     9661 tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc tccagttcct gagtgatttg
     9721 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
     9781 agtgataatc aggcgcacgt cagcgtttcc tttgatggat tagacctggt cttttttatc
     9841 gtcaaccaat gggctggcgt cgtgttctgc ttcgatctct tcagcaggaa gtggggcggg
     9901 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
     9961 gacagacagg ctttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
    10021 aacggtggaa ccgagtttaa agcgacccat ttcctggcct ttcagcagtg ccacagaacc
    10081 gtcgttttcc ccggcaggcc aggtccagcg cttgatgata ccttcgcgcg gcggcgtaat
    10141 ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc gctccgacca gaatctgcgc
    10201 cattgggcca aattcggtat cgaaaaggca aatcacgcgt tcgttacggg caaacagatt
    10261 cggcacgttc tgagcggtga gatggttaac ggaaaagaga tcgcccggca cgtagatcat
    10321 ctcacgcaga ataccgttgc acggcatgtg tacgcgatgg tagtcacgcg gggagaggta
    10381 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc agatagttgc atgccagcag
    10441 ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
    10501 caactggctg ataacgccat cggcaggcat gaccagtaca ttcggatcgg tatcgattgg
    10561 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
    10621 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
    10681 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
    10741 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaagg tagcctccag
    10801 gccattgttt tgtcgttcct gatccggcca acatgccgga tcctgaaaaa aaggggacga
    10861 ttctaacgac ggttagctta attgtcagtc atccgtatca gaaaagtttt tacgcgtttt
    10921 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
    10981 cccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
    11041 atatttacac agacctaaat agtcatggaa ttcgacaaag ccctgagtga tttgttccgg
    11101 ctccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
    11161 cgggaagtga tacagccgag cggcggtcgt ggtgtgctga ccgagccccg agttgtcaga
    11221 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
    11281 accgacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
    11341 tttcagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tgttgcggta
    11401 gatatccatc tgctcgttga cgaacgccat gccttcgtcg tccagcaggt ctatcttgtt
    11461 gagcacgata atcggctcaa tctgcaaggt ttcgcaggcc accaggtaac ggtcgataat
    11521 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
    11581 tatgggtttc acgccgtcgt agaaatccgg acgcgttaac accgaggtgc gctcatgcac
    11641 cgcttccacg atccctttga catttacgcc ttccgccgcc ggtttacccg gacgccagac
    11701 tacgcggtcg ccggttacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
    11761 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
    11821 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
    11881 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
    11941 tactgactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
    12001 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
    12061 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
    12121 gctggtgacc gatgccaatc tgaatattct ggcagaaggg ccgaccattg cagtacatca
    12181 gtccgatgaa cagctggcgc tgatggatga ctggaacgtg cgcacccata ccgccagcgg
    12241 gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg gaagctgaac tggcaacgct
    12301 cgaattctta aaacagtggg tgcctgcggg aaaatcgccg atttgcggta acagcatcgg
    12361 acaggaccgt cgtttcctgt ttaaatacat gccggagctg gaagtctact tccactaccg
    12421 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattctgga
    12481 tggttttacc aagcagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
    12541 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttcctggt cacgtaagcg
    12601 ccggaatcca cttgcggcgg cgtgattttg acgctaaatc gaacgtcttg ctgataattt
    12661 cagcacttga gataaaaacg caaaaaaaac ttttttgggg ggttgcagag ggaaagattt
    12721 ctcgtataat acgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
    12781 gtagta
//
LOCUS       DADLSE010000106        12724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLSE010000106 DADLSE010000000
VERSION     DADLSE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.106"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            8..892
                     /locus_tag="JN692_002978"
     CDS             8..892
                     /locus_tag="JN692_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAY7265238.1"
                     /translation="MLVTLATGRGLMLVGEPGTAKSLLSELLATSISGDAGLTIQGGA
                     STTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITRTPLEVQDCLL
                     GMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKRRFDFETVFPI
                     MDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAI
                     MSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQRVA
                     THKEAHWQAYYQARHRLP"
     gene            903..3182
                     /locus_tag="JN692_002979"
     CDS             903..3182
                     /locus_tag="JN692_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265239.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGNSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSKKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAASAGLAPFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            3175..4311
                     /locus_tag="JN692_002980"
     CDS             3175..4311
                     /locus_tag="JN692_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7265240.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            4308..6311
                     /locus_tag="JN692_002981"
     CDS             4308..6311
                     /locus_tag="JN692_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HAY7265241.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            6436..6897
                     /locus_tag="JN692_002982"
                     /pseudo
     CDS             6436..6897
                     /locus_tag="JN692_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(6938..7408)
                     /locus_tag="JN692_002983"
     CDS             complement(6938..7408)
                     /locus_tag="JN692_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7265242.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTKQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(7455..8174)
                     /gene="yehT"
                     /locus_tag="JN692_002984"
     CDS             complement(7455..8174)
                     /gene="yehT"
                     /locus_tag="JN692_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7265243.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(8171..9856)
                     /gene="btsS"
                     /locus_tag="JN692_002985"
     CDS             complement(8171..9856)
                     /gene="btsS"
                     /locus_tag="JN692_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7265244.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVHLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            10078..10143
                     /locus_tag="JN692_002986"
                     /pseudo
     CDS             10078..10143
                     /locus_tag="JN692_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            10371..10619
                     /locus_tag="JN692_002987"
     CDS             10371..10619
                     /locus_tag="JN692_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7265245.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(10735..11004)
                     /locus_tag="JN692_002988"
     CDS             complement(10735..11004)
                     /locus_tag="JN692_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7265246.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            11061..11729
                     /locus_tag="JN692_002989"
     CDS             11061..11729
                     /locus_tag="JN692_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7265247.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            12045..>12724
                     /locus_tag="JN692_002990"
     CDS             12045..>12724
                     /locus_tag="JN692_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7265248.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT         3238 a         3237 c         3125 g         3124 t
ORIGIN      
        1 cgaacgtatg ttagtcactc ttgccaccgg acgcggtttg atgttggtgg gggaacctgg
       61 caccgcaaaa tctctcctgt ctgaattact ggcaacctct attagcggcg atgccgggtt
      121 aaccatccag ggcggcgcat ctactaccga agatcaaatc aagtacggct ggaactacgc
      181 cctgcttatc aatcatggtc catcaacgga agcgctggtc cctgccccgc tttaccaggg
      241 aatgcgcgac ggcaaaatcg tccgttttga agagataacg cgtacaccgc tggaagtgca
      301 agactgtctg ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga
      361 agccagtcag ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgcga
      421 tcgcggcgtc aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaactgtctt
      481 tccgattatg gatttcgccc aggagctgga gcttgtcgcc agtgcttcgg cgcgtttgct
      541 ggcccatagc ggtattccgc ataaagtgcc tgacgcggta ctggagttac tggtccgcac
      601 cttccgcgat ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac taacagcgat
      661 tatgtccacc gctgaagccg tgaacgtggc ccacgccgta ggcgtccgcg cctggttttt
      721 agcgaatcgc gcaggtgagc cagccgacct ggtggactgc attgccggaa ccatcgtcaa
      781 agataacgaa gaagatcgcg ctcgtctgcg ccgttacttc gaacagcgtg tcgccacaca
      841 taaagaagct cactggcagg cttattatca agcccgccac cgcctgccgt gaagaaagat
      901 gcatgagcga gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg
      961 tgaaatcgtt aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt
     1021 ttaatgaccg ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt
     1081 attgccagta tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat
     1141 tttcgccgga gtggcaggcg ctacaagccg atcgtcgtat tcaggcacaa acttacttca
     1201 tcgatttgcc ttgctgggcg cagagtgaag aagaggacga ttcgcctgat acgcaagagg
     1261 aaagccagac cttgctgctg cgtgccaccc gcatggataa cagcgacacc ctgtgggatc
     1321 acttgttcga agatgaaagc cagcaaactg cattaccctc tgcactggcg cactattttg
     1381 cccaactgcg gggcaactcc cccggcgatg ctctcaatcg tcagcgcgaa gcctttatgg
     1441 cccgctggat tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg
     1501 gctggcacgc tccggcactg gcaaaaatgt ggcgcgaatg tccgcaggta attaacacgc
     1561 cagaattgcc gtcgctggca gatgccgtta caggttgtta tctcaccccc tacagtaaaa
     1621 agcgccttga tgtgctggca ggataccttt caggaatgcc tgccccggtc tggcaaaact
     1681 ggtgctggca gtggggctta cagcaggcgg gagaacaact gctaaaaacg gttctcaccc
     1741 gtttgcgcca gcacaagttg cctgcttcga ccgcggatat ggctgccgct catctgcatg
     1801 cgatggcact ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg
     1861 ccatagcagg atcgctgatt aaagaagccc tgaatgcgcc gttgccgtgg agctatcgcg
     1921 gcgttattca tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg
     1981 acggattcgg taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa gatgtcactt
     2041 gcgaactgga acgtaccgca atctctcttc cggcggagct taccttaaat cgctttaacc
     2101 ccaatggact agcgcaaagt caggtgttac atcggctggc aatactggag atcccaggga
     2161 ttgtacgcca gcagggaagc acactgacac ttgcaggcaa cggtgaagaa cgctggaaat
     2221 taacccgccc gcttagccag catgcggcat tgattgaggc cgcctgcttt ggtgccacac
     2281 tccaggaagc cgcacgccat aaattagaag ccgatatgct ggacgcgggt ggaatcggca
     2341 gcattaccac atgtcttagc caggcggcgt cagcgggtct ggcgcccttc agtcaacaat
     2401 tactggagca actcacatta ttaatcgccc aggaaaatca atttgccgaa atgggccagg
     2461 cgctggaagt gctatatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc
     2521 agatattaca aacgacgtta tgcgcggcta tcgatcgcac gctgtggctg tgtgaatcca
     2581 acggcagacc ggatgaaaag gagtttcacg ctcacttgca tagctggcaa gcgttgtgtc
     2641 atatcctgcg cgatctacat agcggtgtgc aactccccgg cgtttcgctt tctgcggcgg
     2701 tagccttgct ggagcgacac agtcaggcga ttcatgtcct ggcactggat cgcggcgcga
     2761 ctcttggtgc gctgatgcgt ctggaacatc ccaacgctag tgccgaagcg gcgctgacga
     2821 tgctggcgca gttatccccg gcacaatccg gcgaggcgct gcacggtttg ctggcattag
     2881 cccgtcatca actggcctgt cagccggcat ttatcgccgg tttcagcagt catttaaatc
     2941 aactaagtga tgccgatttt accaatgccc tgcccgattt acgcgcggca atggcctggc
     3001 taccgccacg agaacgcggg acgctggcgc atcaggtgct tgagcattat caactggcgc
     3061 aacttcccgt ttcggcactg caaatgccgt tgcattgtcc accgcaagcc attgcacatc
     3121 atcaacaact cgaacagcag gcactggcat cgctgcaaca ctggggagtt ttccatgtct
     3181 gaactgaacg atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa
     3241 gcggcagaaa cgacgctttg tgggctggat gacaacgccc ggcagataga tcacgcgctg
     3301 gagtggctgt atgggcgcga tcctgaacgg ctccagcgtg gtgaacgctc tagtggatta
     3361 ggtggctcaa atctcaccac tcctgagtgg atcaacagta ttcacacgct gtttccgcaa
     3421 caggtgattg agcggctgga aagcgatgcc gtactgcgct acggcattga agatgtggtg
     3481 acaaatctcg acgtgctgga acgtatgcag ccttccgaaa gcctgctacg cgccgttttg
     3541 cacaccaaac atctgatgaa ccccgaagta ctggctgccg cccgcaggat tgtgcgccag
     3601 gttgtggaag aaattatggc gcgactggca aaggaagttc gtcaggcttt ttctggtgtc
     3661 cgcgatcgtc gtcgccgctc atttattcca ctggcgcgaa actttgattt caaaagtact
     3721 ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat cgaattcccc
     3781 cgctttaaca gccgcattaa acgccaaagc gagcaatggc aactggtctt actggttgat
     3841 caaagcggat cgatggtcga ttcggtgatc cactctgcgg tgatggcggc ctgtttatgg
     3901 cagttacccg gcattcgtac ccatctggtg gcgtttgaca caagtgtcgt tgatctcacg
     3961 gcagacgttg ccgatccggt agagttatta atgaaagtgc aattaggcgg cggaaccaat
     4021 atcgccagtg ccatggagta tggtcggcaa cttattgaac aacctgcaaa aagcgtcatt
     4081 atcctcgtga gcgattttta tgaagggggt tcatcatcat tgctgacgca ccaagtgaaa
     4141 aagtgtgtcc agagcggcat caaagtgctg ggactggcag cgctcgatag cacagcaaca
     4201 ccttgctatg accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc
     4261 atgacaccgg gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac
     4321 gtccggaatt attagaactg acaccgcagg cactgacggc gttaagcaat gccggttttg
     4381 ttaagcgcag ccttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc catgagaaca
     4441 gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac
     4501 tgaaagaggc tcagtgcacc tgtggggcca gcggcatgtg ccgtcatcgc gtgatgctgg
     4561 tgttaagtta tcaacgactt tgtgctaccg cccagcctac gggaaaagaa gaggaatggg
     4621 atccggcaat ctggctggaa gaactggcta cccttcccga tgccacccgc aaacgcgcac
     4681 aggcgctggt cgctaaaagc atcaccattg agttgttctg tgcgccgggt gaaattccct
     4741 ctgcccgctt accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc
     4801 gttgtgattg tattgaaggc acactttgcg aacatgtcgt actggcagta caggcctttg
     4861 tccaggcaaa agcgcagcaa gcggaattga ctcatttaat ctggcagatg cgcagcgaac
     4921 acgtcacatc atctgacgat ccgtttgcca gcgaagaagg caaaacgtgt cgtcaatatg
     4981 ttcaacaact aagccaggca ttatggctga gcggcatcag ccagccgctt attcactacg
     5041 aggccgcttt cagccgcgcg cagcaagcag cggaacgctg cagctggcga tgggtgagtg
     5101 aatcgctacg gcaactgcgc gccagcgttg atgccttcca cgcccgcgcc agccactatc
     5161 atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag
     5221 agatggcccg gagcgacagt gttggtgaag ttcctcctgt gccgtggcgc acggtcgttg
     5281 gctctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc
     5341 gttgctggca ggatattgag cattatggtt tacgcatctg gtttaccgat cccgacaccg
     5401 gcagtatttt gcacctttcg cgcagttggc cgcgaagtga acaggaagac tcaccggcag
     5461 ctacgcgtcg gctgtttagt tttcaggctg gcgcactggc gggtgggcaa attgtttcac
     5521 aagcagcaaa acgcagtgcc gatggcgagc tgctgttagc tacccgcaac cgcttaagca
     5581 gcgttgtgcc gctgtcgcct gatgcctggc aaatgttgag cgcgccgtta cgccagccgg
     5641 gcattgtggc tttgcgggaa tatttacgcc agcgtccccc cgcctgcata cggcctctta
     5701 atcaggtcga taacttattt attctgccgg tcgctgagtg tatttcgctc ggttgggata
     5761 gcagccgcca gacgctggat gcgcaggtaa tcagcggcga aggtgaagat aatctgctga
     5821 cgttatcact gccagcctca gccagcgcgc cttatgccgt tgaacgcatg gcggcgcttt
     5881 tgcaacaaac agacgacccc gtgtgtctgg tttctggctt tgtcagtttt gttgatgggc
     5941 aattgacact ggaaccacag gtaatgatga caaagactcg tgcctgggcg ctggacgcag
     6001 aaactgcgcc tgtggtggta tcactacctt ctgccagcgt tctgcctgtg ccgtctactg
     6061 ctcatcagtt gctgatgcgc tgccaggcgt tacttattca actgctccat aacggctggc
     6121 gctatcagga acagagcgct attagtcagg cagagttgct ggcgaatgac ctcaccgcgg
     6181 tgggttttta tcggctggca catgtgttgg cacaatttcg taatacagaa agcgaggcac
     6241 gggtagaagc aatgaataac ggtgttttgc tttgcgaaca attattcccc atgcttcagc
     6301 aacaaggatg aaatagtgct ttttactaag agttctactc cagttccgga ctgctcacgc
     6361 cacggtatta ggcatatcct atatagcccc tggtgagagt caccagtttc ttgattaaat
     6421 aaaatggagt tttacatgaa ggctttcaat aagctgtttt ccctcgttgt tgcatctgtt
     6481 ctggttttct ctcttgctgg ctgcggtgac aaagaagaat cgaagaaatt cagcgccaat
     6541 ctgaacggca ctgaaattgc cattacctat gtctacaaag gtgacaaggt gcttaagcaa
     6601 tcttctgaaa ccaaaattca atttgcctcc attggtgcaa ccaccaaaga agatgctgcc
     6661 aagacacttg agccgttaag cgccaaatac aaaaacatcg cgggtgttga agaaaaatca
     6721 acctataccg atacctacgc gcaggaaaac gtgactatcg atatggaaaa agtggatttt
     6781 aaagccctgc agggtatttc atgaatcaac gtttctgctg aagatgccaa aaaaggtatc
     6841 actatggcgc aaatggaact ggtgatgaaa gccgctggtt ttaaagaagt gaaataatct
     6901 gtcggcggcc atgtttcgca tggccgccat acccgtctta gcttttcttc acatgctggc
     6961 gcgctgccag tccacgcaga aaataacgta aaaattgatc gccgcattcg cggaagtttt
     7021 tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat cgaaacgcgg aactgctgtt
     7081 tggtgaggat agccagaatg tcatcggttt tcagcgaaaa cgcgatgcgt aattttttca
     7141 gcacgatgtt gttattaatg cgacgttccg gctccagtgc cggagcagac tcatccttgc
     7201 cgcgtttttc ataaatcagg ccattgagga atgacgacaa aacaatgtcc ggacaacgct
     7261 gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat ctgttccgcg gtggcttcga
     7321 cattatccag cgccagaata cgcaccaggt cattattatt ggctttcaaa atgtagcgca
     7381 cgctgcgcag aatatcgtta cttagcatga ggccttcagg tgttgatgag gcaaaaagcc
     7441 attttagcag tcttttacag gccaatcgcc tcttttaagc ttttcagata acggcggctg
     7501 accggcacgg ttaaaccatt acgcaaaatc aactcggcct ggccgttatc ttccaaacga
     7561 atctcctgta aatgcgcgag gttaaccaga tactgacgat ggcagcgcag tagtggtgta
     7621 cgactttcca gggtacgtaa tgtcaattcg gtaaagccct ctttcccttc gtggctggta
     7681 acgtagacac cgctcatccg actgctgaca aatgccacat ctttcatttg cagcaaataa
     7741 atccgactat gccccgtaca agggataaat ttcagcgcct gttgattttc cggtaacagc
     7801 gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca gcgttttctc cagtcgcgct
     7861 tcatcaattg gcttcagcag ataatcaaag gcatgttctt caaaggcttt aatagcgtat
     7921 tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt ccgggtcaag catccccacc
     7981 atttccagac cactgatgcg cggcatctgg atatcgagaa acagcacatc cgggcgcagt
     8041 ttatgcaccg cgccgatccc ttccacggcg tttgaacact ctccaacgat ttcaatatcg
     8101 ctctgctcct gcaaaaatat acgcaggttc tcccgtgcta acggttcatc atcaacaatt
     8161 aagactttaa tcatgcctcg tccctccatg gtagtcgtaa cgttattcgg gtgtaactat
     8221 caggctcaca ggcgacgctt attccataat catcgccaaa ccgttcacgt aaacgcttat
     8281 ccaccagatt catccccagc ccactggcat tggttaccgg ttgatacaaa ccggcattgt
     8341 cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc gctgattgcc actcgccctg
     8401 tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc cactatcggt tgcagggtaa
     8461 acgcgggcaa ttgctgctgg gataattctt gcggaatagc aatgttgacc tgcaaccgcg
     8521 actggaagcg cgccttttca atttgcagat aagcgttcac atgttcaatt tcgtcggcga
     8581 gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa aaaagtggaa agatactgca
     8641 ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc tttaatggtg ttaagcgcat
     8701 taaacaaaaa atgaggattc acctgggcgt gaagcagttt gatttctgac tgggtgagca
     8761 tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc cgaaagcagt tgcgcaatcc
     8821 cctcgcccag cgtgcggttt attgaactga ataaacggtt tttggcttca tacaatttga
     8881 tggtgcccat cacacgctga ttttcgccac gcaacggaat taccagcgtc gaccccagtt
     8941 tgcattgcgg atgcaaagag caacgataag gtacttcgtt gccatcagcg tagaccactt
     9001 caccggtttc aatcgctttt aaggtgtaag tcgaagaaat cggtttgccg ggtaaatggt
     9061 ggtcgtcacc aattccggta aaggccagca atttctctcg atcggtaatc gcgactgcac
     9121 caatatccag ctcctgatac agcacctgag ccactttcat gctgttcact tcgttaaacc
     9181 cctgtcgcaa aatgccttcc gtcgaggctg ccactttcag cgcagtggca gaaaaagccg
     9241 aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat acgcataaac agcgccgcgc
     9301 cgacggtatt ggtgaccatc attggcgcag caatattact caccagatgc accgcatctt
     9361 cataaggtcg ggcgatcgca aggatgatca gcatttgcac catttcagcg acgaacgtga
     9421 cggcaccggc ggtaatgggg ttaaagactt tatcagtgcg cccgcgacgg atcaggatgc
     9481 tgtgtaccag gccgccgagc aatccttcaa cgatggttga gatcatgcaa cttagcgcgg
     9541 tcatgccccc catcgaatat cgatgtaagc cgccggtcag accaaccagc ccaccgacga
     9601 ccggaccgcc gagtaagccg cccattaccg cgcctatcgc acgggtattg gcaatagaat
     9661 cgtcaatgtg caacccaaac caggtgccca tgatgcagaa gatggaaaag acgatgtagc
     9721 agagaaattt atgcggcaga cgaaccgtga cctgcattaa cggtatgaat aatggcgttt
     9781 tactcattaa ccacgcaatg actaaaaaaa cgcacatctg ctgaagcagc agcaacacca
     9841 gattaaaatc gtacataccc gcaaaccaca cttcccttta aaacgcgtaa catacattgc
     9901 ctgcgtttaa ctttctttga actcttgcag aaaaatgaga attcgtgagt acgatcactc
     9961 aaaatcgcct ggcaaaaata aaatcaccct atagatgcac aaaaaacggg caaaactacc
    10021 tggttcgcaa aactgcgtct aaagttaaac tgggacctcg cgagcaaggg tgagatgatg
    10081 gcgctttaca caattggtga agtggcgttg ctttgtgata ttaatcctgt cacgttacgc
    10141 gcgtgacgca acatttttaa aaatcgacga agatcgtcca ggagcgccgt ttttcaaggg
    10201 ttggatagat gattcgttta ccatgtcgac gaacttcgac gaatttcgcc gataaaaagt
    10261 gtcccataca tgccccattg atgccccata aacaatatca ggaattgaca ctggttatcc
    10321 atacagataa aaataacacc tgtacataac tacagttaca ggagggtgtt atgcgtgttg
    10381 agatctgtat cgctaaagaa aaaatcacta aaatgccaaa cggtgctgtg gatgcgttaa
    10441 aggaagaatt aacccgacgc atcagtaaac gttatgacga tgtagaggtg atcgtaaaag
    10501 ccaccagcaa cgatggcctt tctgttacac gcaccgcaga taaggattct gcaaaaacat
    10561 ttgttcagga gactctgaaa gatacctggg agtctgctga cgagtggttt gttcactaat
    10621 aaacacgtaa aatcggtaac ggctggaaat cattcaatac tcgcactatc gaaagtttac
    10681 cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat
    10741 tgtgtctggt tcttccggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag
    10801 caacacccga tacttcttcc aggcttccag caacgagttt tcttcctccg ttgcgatctc
    10861 cagatctaca gcatcctgaa gtggcgcaat atgctcactg aatccctgga tgtagaactg
    10921 tgtggtgacg gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc
    10981 aatatcgcta tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa
    11041 ttcacaaata aaactataat atggtgaaat taatgaaaaa aaacacagat gatggggcta
    11101 aaatttacac accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc
    11161 ggcttgcatg gggatgtcct acaaaggaac accttcttcc acactttctg gaacatttag
    11221 gtaacaacca tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga
    11281 gtagtctgat atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg
    11341 ctggggaatc aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcctgcgg
    11401 cgttacatgg tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa
    11461 atatatctac aaaaagctgt gtaatacgca atgcggcgca ggccttaact gatgatggaa
    11521 ctctatacgg agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac
    11581 tgatgcgcat ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct
    11641 taacacatat actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag
    11701 taatgttttc cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac
    11761 gtgctacggt ttcctttacg atttggggtt gcgactttac ctctattgat aatgcattcc
    11821 ggcagaacgt tcaaatatga acgttcgata tttaactaac cgaaaaacaa aatatagcac
    11881 aggcgagaca tttaccatct aagaaaatag tatcgttttt ttacagttaa atcaacatca
    11941 cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca ttttatttca gtttattatt
    12001 tcccagtatc acattttatc agaatatcct tctgcgaggt tattatgctt cctgtaaata
    12061 atccccccct atccactgga aacgtctctt tttacagaac tacatcaatc gacaatgttc
    12121 acaataatta tctctccgaa tgggttgaat ggactaaaaa cagcatttcc ggagaaaaca
    12181 gggaaactgc ttttacccgg ctccaattat gtctggagaa cagtgaaaca tcgttggact
    12241 tatcttgttt aggtctcaga tctctaccac gattgcctga caatcttgat gaaattaatg
    12301 taagcaataa ccaactatca atgctccccg agctaccaag ggcattgaaa gagctgaatg
    12361 caagcagtaa tcaattatct gcacttcctg aattaccagt gtcgctggaa tatataaatg
    12421 tgagtgataa ccatttgttc gcacttcctg aattacctgc gtcactagaa tatattaatg
    12481 taagtgacaa tcacctgtct gtacttccga ggttaccaat gtcattggaa ttacttgatg
    12541 cagccagaaa tgctttggaa gtaataccag attttccaga aagagatgat catattataa
    12601 gaatattctg gcttaatcag aaccggatca cggcaattcc ggaaagcata cttggcctca
    12661 gttctgatag cgttgtcaat cttagagaaa atcaactatc tcccagaata atgcaaactt
    12721 tgtt
//
LOCUS       DADLSE010000107        12534 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLSE010000107 DADLSE010000000
VERSION     DADLSE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.107"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(15..338)
                     /locus_tag="JN692_002991"
                     /pseudo
     CDS             complement(15..338)
                     /locus_tag="JN692_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
     gene            521..664
                     /locus_tag="JN692_002992"
     CDS             521..664
                     /locus_tag="JN692_002992"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265249.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(883..2421)
                     /locus_tag="JN692_002993"
     CDS             complement(883..2421)
                     /locus_tag="JN692_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7265250.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2586..3737)
                     /gene="yiaY"
                     /locus_tag="JN692_002994"
     CDS             complement(2586..3737)
                     /gene="yiaY"
                     /locus_tag="JN692_002994"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7265251.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLLHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(3927..5771)
                     /gene="selB"
                     /locus_tag="JN692_002995"
     CDS             complement(3927..5771)
                     /gene="selB"
                     /locus_tag="JN692_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7265252.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLGIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5768..7159)
                     /gene="selA"
                     /locus_tag="JN692_002996"
     CDS             complement(5768..7159)
                     /gene="selA"
                     /locus_tag="JN692_002996"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7265253.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQCLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7257..7865)
                     /locus_tag="JN692_002997"
     CDS             complement(7257..7865)
                     /locus_tag="JN692_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7265254.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVLVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFACTKPPKA"
     gene            complement(7936..9021)
                     /locus_tag="JN692_002998"
     CDS             complement(7936..9021)
                     /locus_tag="JN692_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7265255.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHLYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFILEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(9024..9386)
                     /locus_tag="JN692_002999"
     CDS             complement(9024..9386)
                     /locus_tag="JN692_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7265256.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9923..10606
                     /locus_tag="JN692_003000"
                     /pseudo
     CDS             9923..10606
                     /locus_tag="JN692_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            10608..11486
                     /locus_tag="JN692_003001"
                     /pseudo
     CDS             10608..11486
                     /locus_tag="JN692_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
     gene            11486..12073
                     /gene="mtlR"
                     /locus_tag="JN692_003002"
     CDS             11486..12073
                     /gene="mtlR"
                     /locus_tag="JN692_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7265257.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(12085..12294)
                     /locus_tag="JN692_003003"
     CDS             complement(12085..12294)
                     /locus_tag="JN692_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265258.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         3071 a         3373 c         3089 g         3001 t
ORIGIN      
        1 taaatccata agtattactc ggcagaagag gatttgatgt cggcgagctg tttctccagc
       61 ccggcaatac gctgttgtag ttgcacaacg gaggagtcat ggttttacat tccccatccc
      121 cgctccgggc ggtaaagcgt ggcccaaatc cacaaaaatg gccagataac atgcagcgta
      181 aaaagactca cccaaccagc aacatgaatg gcgtcggcat gaggatgatt acgtttttta
      241 gcaatcaggt agggaatatc atgtaaaata ataatcccat agaatatcac cagaaataca
      301 aaaataagca ctcccagtgc aaaatagtcc aggaacatat ttccctcaaa gaatataaaa
      361 aagaacaatt aacgcatatt atgaaatgcc atgatgcagt gcaatttcct tatttatata
      421 acgacatatt gttttttata tactttctga aatgtgctgt ctgatttttc aacaggacga
      481 ggtttatcat attgaaatag tggaactatt ggccaaacta atgaataact ccagtaaaac
      541 atccacagta cagattaagc gtattaaacc ttcaattatc taccgtttat tgctgattgg
      601 cctcggatca ccaatggtga tttacggcct ggttcgcccg ctcaccatcg aaacgcgaga
      661 ttaaactcct gacaaaacac gccccagaaa cgcacttctg ttggcgtagt ctgggttatt
      721 gcgcacgtag gtttaaaacg taaattgtaa caacgtgcgc tttgtttatg ccggatgcgg
      781 cgtgtacgcc ttatccggcc tacggtcagg ttcccgtagg catgataaga cgcgtaagcg
      841 tcgcatcagg caatgaatac ccaatgcgac cagcttctta tatcagaaca gccccaacgg
      901 tttatccgag tagctcacca gcaggcactt ggtttgctgg taatgctcca gcatcatctt
      961 gtgggtttcg cgaccgatac ctgattgttt gtagccacca aacgcagcat gtgccgggta
     1021 agcgtgataa cagttggtcc acacgcgccc agcctgtatg ccgcgcccca tcttataggc
     1081 cagattaccg ttgcggctcc agacgcccgc gcccaggcca tattgcgtat cgttcgccag
     1141 ctccagcgct tcttccatcg ttttgaaggt ggtcaccgcc agcaccgggc caaaaatctc
     1201 ctcctggaac acccgcatat tgttctgacc aaacagaatc gtcggttcga ggtagtagcc
     1261 gtctttcagt tcaccttcca gcagcttgcg ccgcccgcct gtgagcacgt cagcgccctc
     1321 ttttttaccg atatcaatgt agttgaggat ggtttccagt tgcccgtgag aaacctgcgc
     1381 gcccatttgc gtcacgctgt cgagcgggtt accgctgcga atgctttcga cacggcggat
     1441 ggcgcgttcc ataaagcgtt cgtagattga ctcctgcact aaggcacgac tcggacaggt
     1501 gcaaacttcg ccctggttaa aggcaaacag tgcaaagcct tccagcgctt tatcgaaaaa
     1561 ggcatcttct tcatccatca catcagcaaa gaagatattt ggcgacttac cgcccaactc
     1621 cagcgtcacc ggaataatgt tttgcgttgc gtattgcata atttgttggc ccacttccgt
     1681 tgagccggta aacgccactt tggcgatgcg tttcgaggtc gccagatatt cgccaatttc
     1741 cccacctgcg ccattgacca cgttcaccac gcccggcggc agtaaatcac cgacaatttc
     1801 cattagcagc agtacagaaa gcggggtaag acgtgcgggt ttcagcacca cacagttgcc
     1861 cgccgccagc gcgggagcca ttttccagct cgccatcagc agcgggaagt tccacgggat
     1921 aatctgcccc accacgccta acggttcatg gaaatgatag gccacggttt cgctatcaac
     1981 ttcactgatc ccaccttcct gcgcccgaat acacgaggcg aaatagcgga aatggtcaat
     2041 cgccagcggt acatccgcag cactggtttc gcgaatgggt ttgccgttat cccaggtttc
     2101 agctgtcgct aacagctcga ggttttgttc cattcgatcg gcaatcttaa acagaatcgc
     2161 cgcgcgatcc tgcaccgagg tgtgcgccca tttatctttc actttgtgcg cagcatccag
     2221 cgccagatcg atgtctcgtt tgcccgaaga cgccacttcg cacagcagct gcccggtcac
     2281 cggcgtcagg ttctggtaat actcaccatc ggcaggggct acccattcgc cgccaataaa
     2341 gttgtcgtag cgggctttta acttgatggg gaaaccatac tcgccgggct taatctgtgc
     2401 tgaaggggga ttattggtca tgatcgtctc cttgcggtgt gaggtataac aagggtagac
     2461 gtgactggcg aaatattcgc cagtcggtaa cagctttacg acagctatca cgaatttacg
     2521 ggcaaggatt acagagatag ttgaaaaagc ggctgacaat ttgccagccg ttgttggaat
     2581 gatgattaca ttgcggcgcg ataaatcgcc acaatttctt cgtgagttgc ctggatcggg
     2641 ttagtaaagc cacaggcatc tttcagggca ttagtcgcca gaaccgcgaa atcttcttct
     2701 ttcacgttca ggtcgcgtag gcctgccggg atatccactt tcttcgccag ttcacggatg
     2761 gcgttaatgc aggcttcagc accttccgcg tcgtttttac ctgtcacgtt cacgcccatt
     2821 gcagcggcac agtcacgcag acgtgcagcg gcgactttgc tgttgaatac ctgaacatgc
     2881 agcagcagaa cggcgttaca tacaccgtgt ggcaggttgt agaaaccgcc cagctggtgc
     2941 gccatcgcat gaacataacc cagagaagca ttattgaacg ccataccggc aaggaactgg
     3001 gcataagcca ttgcttcacg cgctttcgca ttactgccat cttcaacggc taacggcagg
     3061 ttttcggcaa tcatggtcac ggctttcagt gcacaagcgt cagtgatcgg cgtggcggca
     3121 atagaaacat acgcttcgat agcgtgcgtt aaggcatcca tacccgttgc ggcggtcagt
     3181 gacttcggca taccaatcat cagagaggag tcattgacag aaagcagcgg agtcacgtgc
     3241 ttatcaacaa tcgccatttt gttatgacgc gcttcgtcag tgatgatgca gaaacgggtc
     3301 atttcagagg ccgtacccgc cgtggtattg atggcgatca tcggcagctg cggttttgca
     3361 gagcggtcaa cgccttcgta atcgcgaata tcgccgccat tggctgccac cagcgcaata
     3421 ccttttgcgc agtcgtgtgg agaaccaccg cctaaggaga tcacgctatc gcaattattt
     3481 tctttaagta atttcaaacc tgcggcgacg ttttccgtgg tggggttagg ttgggtgcca
     3541 tcataaataa cgctaaaaat attgcgttct tccagtgctt tttgcacatc acccgccata
     3601 cctaatttcg ttaacatatt gtcagtgaca attaaggtac gggtaaatcc ataatctgcc
     3661 atcatattca ttgcatcagt caatgaatca gcgccgatga cattcactga aggaataaag
     3721 aaagttgaag atgccataac acactcctga aagtgtaaaa aagggtgggc agcttactga
     3781 ggattttcat tcgaaaacat gatcattatt attaattatc aaaattaatc taataaaaag
     3841 cacattgtat tcattaaaca atgtgcttta tttaaaatca taagaaattt gtattaattt
     3901 attttaaaaa ttaattaatc atttccttat ttttccggaa ataataatgc gtcgcgcaat
     3961 aaatgatcat ttccacgacg acgcgtaaag ccaatgcggt caaaatattc cagaatctga
     4021 attcccagct ttcggcctac gcctaagcga tcgcggaaat ccgccgcgca ggttgaacca
     4081 cactcctgat cgagatcgcg gatcatattg gcaaattcga caatccgatc gttacggtaa
     4141 taacgatctt tgacgatcgc ggtaattatt ccttgctgcg ccgcctggcg tagcgtcagg
     4201 cgcattgcct gctcgtcggt acccgtctct tttgccagat cacgcaccca ccacggctcg
     4261 tcaccaaaca gcggctctgc tttttgccag atggcctgct gctcttcgct gaagcccgct
     4321 ttgtgatccg gcagatgcag ccaaccgtga tgactgagga tgtcgccgct ttcgcgcatc
     4381 ttttcaatca gcaacagtac tagcgcttca tcttccattg gcaacgccat acgtcgcaga
     4441 cgttcgcgcc caggcccagg ttcatcgcga tgttgctcat gataagtcgc taatgtgtcg
     4501 agaattttcc gttgccagcg agcggcaacc ggcgcattca acaagctata accagcctga
     4561 atatagccag gctgttgcag cagttcgcgc atcccttcgc cgttgagctg gcgcgcccag
     4621 gcgaaatcag gaagattaac cgcgccgcgt tgcaggtgaa cggctaacgc ttcgctatca
     4681 ttctgtgccc tggcgaggga agccagccat tgcagatact ctggtttacg tttaccgcga
     4741 cgcggcgggt taagcatcac gacgcgcgct ccggccagcg tgttgcgggc agagatatcg
     4801 cgcaatacca ggcggtcgtt atctgccagc cataacgggg tgtcgaagac cagttcagca
     4861 aggttatctt ccagcagtga aacgcgtccc gtgacgtggc tggcggcgtg gtgaatatgc
     4921 agcggctgcc actgggtcag cggtgtatgg gtttgaagct cgacaatcac ccgtgtgaac
     4981 ggctctggcg gcacatcggc aagcagccag tcgccacggt taatctgctc tttttccgca
     5041 tcacccgcga tgtcaagcgc gatacgctgc ccggcattgg cggtttctgt tggctggttt
     5101 tgcgcatgca gcgcacgtac acgcatcggt ttatttacac cagtcagcca gagtgaatcg
     5161 cctaccttca cttccccgct taacgccgta ccggtgacga ccagcccggc accttttacg
     5221 gtaaatgcgc ggtcaatcgc gaggcggaaa ctatgttggc tggcgtgctc gcgttccggc
     5281 aactgaagca gatgctcgcg cagggcatca attccccgac cttcggttgc tgcggtgata
     5341 aacagttttg cctcagcaaa accgtattcc cgcagaacct cctttacctg gcgttcaacc
     5401 tcatcaacac gcgcttcgtc cacgcgatcg gctttggtca gcgccactgt cagcatcggg
     5461 ttaccggtca gctgcaaaat cgccagatgc tcacgggtct gtgccatcac gccgtcatcg
     5521 cacgccacca ccaacagcgc gtgatcgata ccaccaacgc ccgccagcat gttggaaaga
     5581 aacttttcat gaccgggaac gtcgataaaa ccaggcacgc ggccatccgg ctgcggccag
     5641 taggcatagc cgagatcgat ggtcatgccg cgcttttttt cttccggcag acggtcagca
     5701 tttacgccag taatcgcctg caataaggtt gttttgccgt ggtcaacgtg tccggcagtc
     5761 gcaataatca tttcaacaac atctccaaaa accgttgctc atcttcaagg cagcgtaaat
     5821 ccagccacaa tcgtccgtca taaatacgac caatcaccgg cactggcaat tcacgccagc
     5881 gggcggctaa tgactcaagg tggctaccgc gtccatcatg gggtgtaaac gttaatgccg
     5941 cgctcggcag gcgatcaacc ggcagcgaac cactgccaat ctgcgaaaga catggcataa
     6001 cctgtaccgc aaactccgcg ccgtaatgtg cggcaagggg agcctgtaaa cattgtgcct
     6061 ggatttgaat gacctctgcg ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca
     6121 gagcttcagg gtgtaaataa agacgcaacg tggcttccag cgccgcgagg gtcattttat
     6181 ccgcgcgtaa tgcacgcttc agcgggtggc tttgcaggcg ggcgatcatc tcttttttac
     6241 caacgataat ccctgcctgc ggcccgccta acaacttgtc gccggagaag ctcaccagac
     6301 tgacgcccgc cgcaatcaac tcctgcggca ttggctcttt cggcaaaccg tactggctaa
     6361 gatcgaccag cgagccactg cctaaatcag tcaccactgg aacatccagc tctttgccga
     6421 gcgccaccag ttcagcttcg tctatcgctt tggtgaaccc ctgaatgctg tagttactgg
     6481 tatgtacttt catcaacagt gcggtatttt cattcaccgc ctgacgataa tcattcgcgt
     6541 gcgtgcggtt ggtggtccct acttcgtgta gggtacagcc tgcctgacgc ataacatcgg
     6601 gaatacgaaa cgcgccgcca atctccacca gttcgccgcg agataccacc acctctttcc
     6661 cgctggcagt ggccgccaac atcaataaca ccgccgccgc attgttattg acgatacagg
     6721 catcttccgc ccccgtaata cggcacagca gctgcgccag cgcccgatcg cgatgtccgc
     6781 gtccggcgtc gtccagatcg tactcgaggg tcactggcga acgcatagcc tgcgcaacgg
     6841 cttccaccgc tgcttccgcc tgtaaagctc gcccaaggtt ggtatgcagc acggttcccg
     6901 tcaggttgat caccggacgc agcgcgctct gcgcctcttt cgtcaaccgg gcatcgactt
     6961 cttgcgccca gttttcacac cacgcaggca gcgtctggct gccacgaatc acttctcgcg
     7021 cttcgtcgag catctgacgc aacaattcca ccacgcgggt gtgaccataa gtatcacgca
     7081 aagaaaggaa ggagctatcg cgcaataagc gatcaatagc cggaagttga ctatagaggg
     7141 aacgcgtttc ggttgtcata ggaaacctgg ctgatcaagg ccctctcaca cggagaaggg
     7201 cgtttaacat aaccacggat tgtaacgtga gatgggtcag gaggacatat cgcgcatcaa
     7261 gcctttggcg gtttggtgca agcaaaactt tcgcggctaa acaggttttc gaccagtttg
     7321 actaagtgtg ggcggtcgac acaccaacct ggcgcaacgc ggcggaaatt gagatatccg
     7381 acagcacagg caatggcgat agtcgccaga ttgaccgtat cggttttgag tgtgccatcg
     7441 accagatatc cttccagcac atccagactg cggttgattt tctcccgctg gcgtaacaat
     7501 tcatcttcag actgctgcgc cgccggacgc gcctgttcac gcaccgatac cagcccggca
     7561 tccataatgc catccgccag cgcctcaatt ttgcgcaccc gcaacgactc cagcggatcg
     7621 cgcggcaaca tcgccggagc gacattcatt aactcaatat attcagcgat gatcggcgaa
     7681 tcaaaccagc attcgccctc ttcggtcacc agcaccagca ctttccctaa cgggttaaat
     7741 tgcgccacgc cgttatccgc gttatagggc agttcattaa taaattcgaa agttatgccc
     7801 ttttctaaca acagaataga aagtttgcgt acaaacgggc tggtgtagct accgacgagt
     7861 ttcatgccga gtcctttgtg cgaggaaaaa tatcagtatg gctcaggctc acgcaaaaca
     7921 caggtgatat aagtctcaat gatccaaata aagataatgc atccaactgg tcattcttag
     7981 cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg ccacctgggc
     8041 gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa tggttcccag
     8101 cacaccgtcc gtgccgggaa cgaccgttaa tgattgcaat accccctgcg cctgataaga
     8161 accgcctggc acgacaggta aaatactagt caatttgccg tggaacacct gcccaggtag
     8221 cgcgttaaac accacttccg catcatcgcc aggtttcaga cgtaacagcg agttttgccg
     8281 aaattgggcg acaatttgcc gtttttgctc gaggatgaag accatcaccg gacgcagcgg
     8341 caaggcagct gcgtatgtac ctgggcggat cagtacctga gtgacgtagc cattgcttgg
     8401 tgcgcgaatg acagtctgct caagattata ttttgcttca gtaagttgcg ctcttaagct
     8461 cacaatctgc gattgctcgc cgttgaccat actgtcgagc tgactctgga tctgcgcctg
     8521 ctccgccacc gagcctttca ctaacgcatc ctgcgcgagg aaattttgcc gcgcatcgtc
     8581 gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaggt aacgttgata
     8641 atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct gcgcttcagt
     8701 gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag cctgaagtct
     8761 gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc ccttttgaat
     8821 aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg gcgtgatagg
     8881 gatcgctatc actgcctttt gcgcggtaaa agtgtaaagg tggttgtagt tcatcaacaa
     8941 aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg ccacgtaagt
     9001 taaaacaatc aatagatcca taattagatc tccgggaacg tggaatagtc agtctttttc
     9061 tcggcggcga gttgatgctc cagccgggaa attcggtcag aaagtgcggc gatttccggt
     9121 tcagtcgctt tctcctgcga cgcaacatgt gactgcatac cccagccacg ctccggttga
     9181 tagagcgtcg cccagatcca cagaaacggc cagataacat gcagagtaaa caggctcacc
     9241 cagcccgccg tatgaatagc gtcggcatgg gggtgattgc gctttttggc aatcagataa
     9301 gggatgtcat gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact
     9361 cccaacgcga aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt
     9421 tttggctggc agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat
     9481 tctcgccata acaccaaaga ataattttta gaggtgatga gttgcttagt tacataacga
     9541 ttgtatgacg aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat
     9601 cttgtgattc agatcacaaa gattcaacaa accatcaaaa caaaaatgtg acattactca
     9661 catttaaatg ccatttttag cgaaaatcac cgccttgttt cttttttaca caggcgtttt
     9721 gtgatgagtg tcacgtcaat tacctctcta ccccctatat ttatgtgact gatatcacac
     9781 aaaagcccgt cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg
     9841 tccggctacc tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc
     9901 aaacataaga aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg
     9961 tcgtttcctc agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat
    10021 caccgcgtta tttattccaa cagggtggtt accgaacgag acgctggcga agctggtcgg
    10081 gccgatgatc acttatctcc taccgctgct gatcggttat accggtggta agctggtagg
    10141 cggcgaacgt ggcggcgtag tcggtgccat taccaccatg ggcgttatcg tcggcgcaga
    10201 catgccgatg ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca
    10261 cttcgaccgc tgggtagacg gtaagatcaa atccggtttt gagatgctgg tgaataactt
    10321 ctccgcaggc atcatcggga tgatcctcgc tattctggca ttcctcggca ttggcccgat
    10381 tgttgaagcc ctgtccaaaa tgctggctgc gggcgttaac ttcatggttg tccatgacat
    10441 gctgccgctg gcgtctatct ttgttgaacc ggcgaaaatc ctgttcctca acaacgccat
    10501 taaccacggt atcttctcgc cgctgggtat tcagcagtcc catgaactgg gtaaatcaat
    10561 cttcttcctg attgaagcta acccaggtcc aggtatgggc gtgctttgtg ctggaacgta
    10621 ttgctccggc tatcgccaaa gggctggtga aacgcaaaga acaaggtaat gaatccccgc
    10681 tgaacatcat cgcctgtgaa aacatggtac gcggcaccac gcagctgaaa ggtcatgtga
    10741 tgaacgccct gccggaagac gccaaagcgt gggtagaaga gcacgttggc tttgtcgatt
    10801 ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg ctggaagtga
    10861 cggtagaaac tttcagcgaa tggattgtcg ataaaacgca gttcaaaggc gcactgccga
    10921 acatcccagg catggagtta accgacaacc tgatggcatt tgtcgaacgt aaactcttca
    10981 ccctgaacac gggtcatgct ataaccgcgt acctcggaaa actggccggt catcagacca
    11041 ttcgtgacgc tattctcgac gagaaaatcc gcgcggtggt aaaaggtgcg atggaagaaa
    11101 gtggtgcggt actgatcaag cgctacggct ttgacgcaga caaacatgcg gcgtacatcc
    11161 agaaaatcct cggtcgtttt gagaacccgt atctgaaaga tgatgtagag cgcgtaggcc
    11221 gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccactg ctcggtacgc
    11281 tggaatacag ccttccgcac aagaatctga ttcaggggat tgctggtgca atgcacttcc
    11341 gcagtgaaga tgatccgcag gctcaggaac tggcagcact gatcgctgac aaaggtccgc
    11401 aggcggcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta tccgaggcgg
    11461 taaccgctta taaagcaatg caataatggt ggaccaggcg caggacaccc tgcgcccgaa
    11521 taacagattg tcagatatgc aggcaacaat ggaacaaacc caggcctttg aaaaccgtgt
    11581 gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg ccgtcgagct
    11641 tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg attacgcggt
    11701 gaagtatgct gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc tttctgtgcg
    11761 tttaaaactc atttacgggt tgggcgtcat taaccgccag gaatacgaag atgcggaact
    11821 gctgatggca ttgcgtgaag agctaaatca cgacggcaac gagtacgcct ttaccgacga
    11881 cgaaatcctt ggaccctttg gtgaactgca ctgcgtggcg gcgttaccac cgccgccaca
    11941 gtttgaacca gcagactcca gtttgtatgc aatgcaaatt cagcgctatc aacaggctgt
    12001 gcgatcaaca atggtccttt cactgactga gctgatttcc aaaatcagct taaaaaaagc
    12061 ctttcaaaag taagcaacgt ctgcttactg cccctctacc tgcttcggcc gataaagccg
    12121 acgataatac tccagacgtt gtagatataa cggcacgctt tcatcgggta ttccggacgg
    12181 aatcgcgtta cggggaggaa gttttttcag atactcccgg aacgcctggc ttgatgccat
    12241 gaaatctacg gctttatcga taagaagcgg aacgttttca cctaatttgc ccatgatgat
    12301 atctccgtat taccccgcgc cgggaatgcg cggccgccaa ttttagttta ggttcccggt
    12361 aaagatgaca ttaaggaaaa cgtgctgaat cctcaataat catccgctgc tgtttattat
    12421 ctttttctat agatttctta attaatcaac gaattgcgta gtttttgagc atgttaatga
    12481 ttacgttgca gaactattac aatgtcatca gttgtgaaca aagcacctgg tcgc
//
LOCUS       DADLSE010000108        12529 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLSE010000108 DADLSE010000000
VERSION     DADLSE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.108"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..379
                     /locus_tag="JN692_003004"
     CDS             <1..379
                     /locus_tag="JN692_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7265259.1"
                     /translation="QRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAP
                     TADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTP
                     RYDKSAADLAWQRTLKWFDKYLS"
     gene            498..785
                     /locus_tag="JN692_003005"
     CDS             498..785
                     /locus_tag="JN692_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7265260.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            974..2092
                     /gene="hybO"
                     /locus_tag="JN692_003006"
     CDS             974..2092
                     /gene="hybO"
                     /locus_tag="JN692_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7265261.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            2095..3081
                     /gene="hybA"
                     /locus_tag="JN692_003007"
     CDS             2095..3081
                     /gene="hybA"
                     /locus_tag="JN692_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7265262.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            3071..4249
                     /gene="hybB"
                     /locus_tag="JN692_003008"
     CDS             3071..4249
                     /gene="hybB"
                     /locus_tag="JN692_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7265263.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            4246..5949
                     /gene="hybC"
                     /locus_tag="JN692_003009"
     CDS             4246..5949
                     /gene="hybC"
                     /locus_tag="JN692_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7265264.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            5949..6443
                     /locus_tag="JN692_003010"
     CDS             5949..6443
                     /locus_tag="JN692_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7265265.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            6436..6924
                     /gene="hybE"
                     /locus_tag="JN692_003011"
     CDS             6436..6924
                     /gene="hybE"
                     /locus_tag="JN692_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7265266.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMYDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            6917..7258
                     /gene="hypA"
                     /locus_tag="JN692_003012"
     CDS             6917..7258
                     /gene="hypA"
                     /locus_tag="JN692_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7265267.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCRGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            7392..7640
                     /gene="hybG"
                     /locus_tag="JN692_003013"
     CDS             7392..7640
                     /gene="hybG"
                     /locus_tag="JN692_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7265268.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN692_003014"
     CDS             complement(7763..8629)
                     /gene="yghU"
                     /locus_tag="JN692_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7265269.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            8834..10693
                     /gene="gss"
                     /locus_tag="JN692_003015"
     CDS             8834..10693
                     /gene="gss"
                     /locus_tag="JN692_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7265270.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            10985..12484
                     /gene="pitB"
                     /locus_tag="JN692_003016"
     CDS             10985..12484
                     /gene="pitB"
                     /locus_tag="JN692_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7265271.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRSFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT         2899 a         3109 c         3449 g         3072 t
ORIGIN      
        1 gcaacgctat ccgcaagcga caggtaaagt gggtattacc ggattttgct atggcggtgg
       61 cgtatcgaac gcggcggctg tcgcgtatcc ggaactggcc tgcgcggtgc cgttttatgg
      121 tcgtcaggca cccactgccg atgtggcgaa gattgaagca cctttactac tccacttcgc
      181 ggaactggac acccgaatca acgagggctg gcctccttac gaggcggcgt tgaaagacaa
      241 taataaggtt tatgaggctt atatctatcc gggggttaat cacggattcc ataatgattc
      301 cacgccccgt tatgacaaat ctgccgccga tcttgcctgg caaaggacac tgaaatggtt
      361 cgataaatat ctctcctgat aggtttatct cttacgggat tacgtcttaa acaagtatga
      421 aaaaatagcg tgcgcaaaag tcgttctttg cctaaaatat cgttatatat ggcgatatat
      481 aacgaatgag gtgaacgatg aataaccatt ttggtaaagg cttaatggcg ggattaaaag
      541 caacgcatgc cgacagtgcg gttaatgtga caaaattctg tgccgattat aaacgcggtt
      601 ttgtattagg ctactcacac cggatgtacg aaaagaccgg agatcgccag cttagcgcct
      661 gggaagcggg tattctgacg cgccgctatg gactggataa agagatggta atggatttct
      721 ttcgtgagaa taattcctgt tctacgttgc gcttttttat ggccggttat cgcctcgaaa
      781 attgatcaaa catacgtatt atcttgcttt aattaattac actaatgctt cttcccttcg
      841 ttttagcgcc ccgccgcagt atcatgatat cgataaccat aataaatgtg tggtaaatgg
      901 cgcatcgatc gcattattga ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac
      961 ggaataacta taaatgactg gagataacac cctcatccat tctcacggca ttaaccgtcg
     1021 tgatttcatg aagctttgtg cagcattagc cgccaccatg gggttaagta gcaaagccgc
     1081 tgcagagatg gccgaatcgg ttactaaccc gcagcgtccg ccagttatct ggattggcgc
     1141 gcaggagtgc accggttgta cggaatctct gcttcgtgca acgcatccaa cggtagaaaa
     1201 cctcgtgctg gagactatct ctctggaata tcacgaagtg ctttccgccg ccttcggtca
     1261 tcaggtcgaa gagaacaaac ataacgctct cgagaagtac aaagggcagt atgtgttagt
     1321 ggtggatggt tccatcccat taaaagataa cggtatttat tgcatggttg ccggtgagcc
     1381 gattgtggat cacatccgca aagcggcgga aggcgcagca gccattatcg ctattggttc
     1441 ctgctccgcg tggggcggtg ttgccgcagc tggagttaac ccaactggcg cagtcagctt
     1501 gcaagaagtt ctgccaggca aaaccgttat caatattccg ggctgcccgc cgaacccgca
     1561 caacttcctc gcgaccgttg cgcacatcat cacttacggc aaaccgccga aactggatga
     1621 caaaaaccgt ccgaccttcg cctatggccg tctgattcac gaacactgcg aacgtcgccc
     1681 gcacttcgat gctggtcgtt ttgccaaaga gttcggtgat gaaggccacc gcgaaggctg
     1741 gtgcctgtac cacctcggct gtaaagggcc agaaacttac ggcaactgct caacgctgca
     1801 attctgcgat gttggcggtg tgtggccggt ggcgattggt cacccgtgct atggctgtaa
     1861 cgaaaaaggt atcggcttcc ataaaggcat ccatcagctt gccaacgtcg aaaatcagac
     1921 tccgcgttca cagaaaccgg atgttaacgc taaagagggc ggcaacgtct ctgcaggcgc
     1981 tattggtttg ctcggcggtg tggttgggct ggttgccggt gtcagcgtga tggcggtgcg
     2041 tgaactgggt cgtcagcaaa agaaagataa cgctgactca cggggagaat aaccgtgaac
     2101 agacgtaatt ttattaaagc agcctcctgc ggggcattgc tgacgggcgc gctgccgtct
     2161 gtcagtcatg cggctgctga aaaccgcccg ccaattccgg gatcgctggg gatgttgtac
     2221 gactcgacct tgtgcgtagg ctgccaggct tgcgtcacca agtgtcagga tatcaacttc
     2281 cctgaacgta acccgcaagg ggaacagacc tggtcgaaca acgacaaact gtcgccgtat
     2341 accaataata tcattcaggt gtggaccagc ggcacagggg tcaacaaaga ccaggaggag
     2401 aacggctacg cgtacattaa gaaacagtgt atgcactgcg tcgatccgaa ctgtgtctct
     2461 gtgtgcccgg tctctgcact gaaaaaagat ccgaaaaccg gcattgtcca ttacgacaaa
     2521 gacgtgtgca ccggttgccg ttactgcatg gtcgcctgtc cgtacaacgt gccgaagtac
     2581 gactacaaca acccgtttgg tgcgctgcat aagtgcgagc tgtgcaacca gaaaggtgtg
     2641 gaacgtctcg ataaaggcgg tctgcctggc tgcgtagaag tgtgcccggc gggcgcggtg
     2701 attttcggta cgcgtgaaga gctgatggcg gaggcgaaaa aacgtctggc gctgaagcct
     2761 ggcagcgaat accactatcc gcgtcagacg ctgaaatctg gcgacactta cctgcatacg
     2821 gtgccgaaat attatccgca tctgtacggc gagaaagagg gcggcggtac tcaggttctg
     2881 gtactgacgg gtgtgcctta tgaaaatctc gacctgccga aactggacga tctttctacc
     2941 ggtgcgcgtt ccgaaaatat tcaacacacc ctgtataaag gcatgatgct accactggct
     3001 gtgctggcgg gcttaaccgt gctggttcgt cgcaacacca aaaacgacca tcacgacgga
     3061 ggagacgatc atgagtcatg atccacaacc gctgggcggc aaaatcatca gtaaaccggt
     3121 catgattttt ggaccgttaa tcgtcatctg tatgctcctg attgtgaagc gtctggtgtt
     3181 cggtctgggc tctgtctctg acctgaacgg cggcttcccg tggggcgtgt ggatcgcgtt
     3241 tgacctgctg attggcaccg gctttgcctg tggcggctgg gcgctggcgt gggcggtata
     3301 cgtctttaac cgtgggcaat accatccgct ggtgcgtccg gcgctgttgg cgagtctgtt
     3361 tggttactca ctgggtggct tgtcgatcac tatcgacgtg ggtcgctact ggaacctgcc
     3421 gtacttctac attccgggtc acttcaacgt gaactcggta ctgttcgaga cggcggtctg
     3481 tatgaccatc tacatcggcg tgatggctct ggagtttgct ccggcactgt ttgaacgtct
     3541 gggctggaag gtgtcgctac aacgtctgaa caaggtgatg ttcttcatta tcgcgctcgg
     3601 tgcgctgctg ccgaccatgc accagtcttc aatggggtcg ctgatgatct cggcgggcta
     3661 caaggtgcat ccgctctggc agagctatga aatgttgccg ctgttctcgc tgctgacggc
     3721 gttcatcatg ggcttctcga ttgtcatctt tgaaggttca ctggtgcagg cgggtctgcg
     3781 tggcaacggt ccggatgaaa agagcctgtt cgtcaagctg accaacacca tcagtgtgtt
     3841 gctggcgatt ttcatcgggc tgcgctttgg cgagcttatc tatcgcgaca agctgtcgtt
     3901 agcgtttgcc ggtgacttct actccgtgat gttctggatt gaagtcctgc tgatgctctt
     3961 cccgctggtc gttctgcgtg tggcgaagct gcgtaatgat tcccgcatgc tgttcctgtc
     4021 agcactgagc gcactgttag gttgtgcaac ctggcgtctg acctattcgt tggtggcatt
     4081 caacccgggc ggcggttacg cctacttccc aacctgggaa gaactgttga tttctattgg
     4141 ttttgtggct attgagattt gcgcttacat cgtactcatt cgtctactgc cgatacttcc
     4201 tcctttaaaa caaaacgatc ataatcgtca tgaggcgagc aaagcatgag ccagagaatt
     4261 actattgatc cggtaacccg tattgagggg catttacgca tcgattgcga aatcgaaaat
     4321 ggcgtcgttt cgaaagcatg ggcttccggt accatgtggc gcggcatgga agagatcgtg
     4381 aaaaaccgcg atccgcgcga tgcatggatg attgtgcaac gtatctgtgg cgtatgtact
     4441 accactcacg cgctgtcttc cgttcgtgcg gcagaaagtg cgctgaatat cgacgttccg
     4501 gttaacgcgc aatacatccg taacatcatt ctggctgcgc acaccacgca tgaccatatc
     4561 gttcatttct atcagctttc ggcgctggac tgggtggaca tcacttctgc actgcaagct
     4621 gacccaacca aagcctccga aatgctgaaa ggcgtttcga cctggcatct gaacagtccg
     4681 gaagagttca ctaaagttca gaacaagatc aaagatctgg ttgctagcgg tcagttgggt
     4741 attttcgcca atggctactg gggtcacccg gcgatgaaac tgccgccgga agtgaacctg
     4801 attgcggtag cgcactacct gcaggcgctg gagtgccagc gtgacgctaa ccgcgtcgtg
     4861 gcgctgctgg gcggtaaaac gccgcacatt cagaacctgg cagtgggtgg tgtcgcgaac
     4921 ccaatcaacc tcgacgggtt gggcgtgttg aaccttgagc gcctgatgta catcaagtct
     4981 ttcatcgaca aactgagcga ctttgttgag caggtttata aggttgatac cgcggttatc
     5041 gccgcgttct acccggaatg gctgacgcgc ggtaaaggtg cggtgaacta cctgagcgtg
     5101 ccggaattcc cgaccgacag caaaaacggc agcttcctgt tcccgggcgg ctacattgag
     5161 aatgcggatc tgtcctcgta tcgtccgatc acttctcatt ccgatgaata cctgatcaaa
     5221 ggcattcagg aaagcgcgaa gcactcctgg tataaagacg aagcgccaca ggcgccgtgg
     5281 gaaggtacca ccattccggc ttatgatggt tggtctgacg acgggaaata ttcctgggtg
     5341 aaatcaccga ctttctacgg caaaacggta gaagtgggtc cgctggctaa tatgctggtg
     5401 aaactggcgg caggtcgcga atctacccag aacaaactga atgaaatcgt tgcgatttat
     5461 cagaaactga ctggcaacac gctggaagtg gcacagctgc actccacgct gggccgtatt
     5521 attggtcgta ccgttcactg ctgtgaattg caggatatcc tgcaaaacca atacagtgca
     5581 ctgatcacca atatcggcaa aggcgatcac accacctttg tgaagccgaa cattccggca
     5641 acgggtgagt tcaaaggtgt tggcttcctc gaagcgccgc gcggtatgct ctctcactgg
     5701 atggttatta aagacggtat catcagcaac taccaggcgg ttgttccatc aacctggaac
     5761 tctggtccgc gtaacttcaa tgatgacgtc ggtccttacg agcagtcgct ggtgggtaca
     5821 ccggttgccg atccgaataa accgctggaa gtggtgcgta ccattcactc cttcgacccg
     5881 tgcatggcct gtgcggtaca cgtagtggat gccgacggca acgaagtggt ttcagtgaag
     5941 gttctgtaat gcgtatttta gtcttagggg tcggcaatat tttgctgacc gatgaagcca
     6001 tcggcgtgcg gattgtcgaa gcgttagagc aacgatacat tctgccggat tatgttgaga
     6061 tcctcgatgg cggcacggcg ggaatggagc tgcttggcga catggcaaat cgcgatcatc
     6121 tgattattgc ggatgccatt gtgtcgaaaa agaacacgcc gggaacgatg atgatcctgc
     6181 gggatgaaga agttccggcg ttgtttacca acaaaatctc tccgcatcag cttggcctgg
     6241 ccgacgtatt gtcggccctg cgcttcaccg gtgagtttcc gaaaaagctg accctggtcg
     6301 gcgtgatccc ggaatcgctg gagccacaca tcggcttaac gccgacggtt gaagcaatga
     6361 ttgaacctgc gcttgagcag gttctggctg cgctgcgcga atcaggcgtg gaagccatcc
     6421 cacgggaggc gattcatgac tgaagagata gcaggtttcc agacctcccc gaaggcgcaa
     6481 gtacaggcag cgtttgaaga aattgcccgg cgttcgatgt acgatctctc ttttctgcat
     6541 ccttcaatgc cggtgtatgt ttctgatttt acgctgttcg aaggtcagtg gacggggtgt
     6601 gtgatcaccc cgtggatgct gagtgcagtt atcttccccg gcccggatca actctggccg
     6661 ctgcgcaaag tgagtgaaaa aattggtctg caactgccgt atggcaccat gacctttacc
     6721 gttggcgaac tggacggtgt ttcgcaatac ctctcctgtt cgctgatgtc gccgctttcg
     6781 cacagcatgt cgattgaaga aggccaacgc ctgacggatg actgcgcacg aatgatcctt
     6841 tcgttgccag tcaccaatcc ggatgtacca cacgcagggc gtcgcgccct gctgtttggc
     6901 cgcaggaggg gcgaaaatgc atgagttgtc gctttgccag agcgccgttg aaattatcca
     6961 acggcaggcg gagcagcacg atgttaagcg cgtcaccgcc gtgtggctgg aaattggcgc
     7021 gctctcctgc gttgaggaga gcgccgtccg ttttagtttt gaaattgtct gccgcggaac
     7081 ggtggcgcaa gggtgcgatt tacatatcgt ctataaaccc gcccaggcct ggtgctggga
     7141 ttgcagccag gtggtggaga ttcatcagca cgatgcgcag tgtccgctct gtcacggcga
     7201 gcggttgcgt gtcgataccg gcgattcgct gatcgtcaaa agtattgaag ttgaataaga
     7261 ctgaggacaa cgcctaaaaa gcgcacggac ggtcccctcg cccctccggg gagagggtta
     7321 gggtgagagg gaacacccgc taaaggttct ttttcgcgtt tgtccggcaa tcgaaataac
     7381 cggagttaac aatgtgtatt ggcgttccag gccaggtgct ggctgtcggt gaagatattc
     7441 accagcttgc gcaggttgaa gtatgtggta tcaagcgcga cgtgaatatc gccctgattt
     7501 gcgaaggtaa ccctgccgat ctgctgggcc agtgggtgct ggtacacgtc ggatttgcca
     7561 tgagcatcat cgacgaagat gaagccaaag ccacattaga cgcactgcgc caaatggatt
     7621 acgacattac cagcgcgtga tgattagctt ttgtaggcct gataagacgc ggcagcgtcg
     7681 catcaggcat tgtgcacgat tgccggatgc ggcgtgaacg ccttatccgg cctggatggc
     7741 ttgctgcgac gaacaccaac ccttacccct gacgcttatc ttctgtattt gtctcaaaat
     7801 cactggcgtc atggcgctca tgcaactgct cgttcagcgg cccgttggtg cggttaacaa
     7861 tacgcccacg tttcactgcc ggacgttcgc ccacttcttt cgcccagcgt tgcacatgtt
     7921 tataacttcc cgcatccaga aactctgcgg catcatacac gccgccgagc accacgttgc
     7981 caaaccacgg ccaaatcgcc atatccgcaa tggtgtactc atcgcccgca acaaacttat
     8041 gctgcgccag ttgcttatcc agcacgtcga gcaggcgctt ggcttccatg gtaaagcggt
     8101 tgatggcgta ctcaattttc accggcgcgt aatggtaaaa gtgaccaaaa ccaccgccga
     8161 ggaacggcgc ggcaccctgt aaccagaaca gccagttcaa cgtttcagtc cgttttgcca
     8221 gatcctgcgg caggaagtag ccaaatttct ccgccagata aagcaggatc gaaccagatt
     8281 caaacacgcg gatcggcgga ttatgcgtat gatcgcgcag cgccgggatc ttcgagtttg
     8341 ggttcacttc gacaaagccg ctggagaatt gatcgccatc gccaatacga atcagccagg
     8401 cgtcgtactc tgcaccagta acgcccagcg ccagtagctc ctcaagcata atcgttactt
     8461 tctgaccgtt cggcgttccc agcgaataaa gttgcaatgg atgtttgcca acgggcagcg
     8521 ttttttcatg cgtcggacca gaaaacgggc gattgatatt ggcgaacgcg ccgccagcgg
     8581 atttatccca cgtccagact ttcgcgggct gataagtatt gtctgtcata gtgagttgcc
     8641 ttctgagtgg tagtgttgaa gcagtgtagc aggtcacttg ctgtacagaa tgtgaatttt
     8701 ttaacccttt gcgccagccg ctgcgatgag gtgtatgatt agtctgacct ctgtccggtt
     8761 aatgtcgctg agcaggtaat cagagcacaa caattacgcc aactgcttag gttgtattgt
     8821 taaaggtaaa gtgatgagca aaggaacgac cagccaggat gccccgttcg ggacattatt
     8881 gggctacgcc ccaggtgggg tagcaatcta ctcttcagat tacagttctc tcgatccgca
     8941 ggaatacgaa gatgacgccg tattccgtag ctatatcgac gacgaatata tgggccacaa
     9001 gtggcaatgc gttgaatttg ctcgccgttt tctctttctg aattacggtg tggtctttac
     9061 tgacgtgggt atggcgtggg agattttctc gctgcgcttc ctgcgagaag tggttaatga
     9121 caacatcctg ccattgcagg catttcctaa cggctcgccg cgtgcgccgg tcgcgggtgc
     9181 gcttcttatc tgggataaag gcggtgaatt taaagacact ggccatgtcg ccatcattac
     9241 ccaattgcat ggcaacaaag tccgtattgc ggaacagaac gtgattcatt ccccgttgcc
     9301 gcaagggcaa cagtggacgc gcgagctgga gatggtggtc gaaaacggct gctataccct
     9361 taaagacact tttgatgaca ccaccattct gggctggatg atccagacgg aagatactga
     9421 atacagctta ccgcagccgg aaattgcagg cgagctgctg aaaatcagtg gagcgcgtct
     9481 ggaaaacaaa ggccagtttg acggtaaatg gctggatgaa aaagatccgc tacaaaacgc
     9541 ctatgtgcag gccaacggtc aggtgatcaa tcaggatccg tatcattact acaccattac
     9601 cgagagtgcc gagcaggagc taattaaagc caccaacgag ctgcatctga tgtatcttca
     9661 cgcaaccgac aaggtgctga aagatgacaa cctgctggcg ctgttcgaca ttccgaaaat
     9721 cctctggcca cgtttgcgtc tctcctggca gcgtcgccgt caccatatga tcactggtcg
     9781 tatggatttc tgcatggatg agcgtggcct gaaggtttac gaatacaacg ccgactccgc
     9841 ctcctgtcat accgaagcgg gcttgatcct cgaacgttgg gcggagcagg gctataaagg
     9901 caacggcttc aatccggcgg aagggctgat taacgaactg gctggtgcct ggaaacacag
     9961 tcgtgcacgt ccgtttgtcc atatcatgca ggacaaagat atcgaggaaa actatcacgc
    10021 gcagtttatg gagcaggcgc tgcaccaggc gggctttgaa acgcgtatct tgcgcgggct
    10081 ggatgaactg ggctgggatg ctgccgggca actgattgat ggggaagggc gactggttaa
    10141 ctgcgtgtgg aaaacctggg cgtgggaaac cgcgtttgat cagattcgtg aagttagcga
    10201 ccgtgagttt gctgcggtgc caatccgtac cggtcatccg caaaacgaag tgcgtcttat
    10261 cgacgtattg ctgcgcccgg aagtgctggt ctttgagccg ctgtggacgg tgatccccgg
    10321 caacaaagcg attctgccga tcctctggtc gctgttcccg caccatcgtt acctgctgga
    10381 taccgatttc actgttaatg atgaattggt gaaaacaggt tacgcagtga aaccgatcgc
    10441 cggtcgctgt ggtagcaata tcgacctggt cagccatcat gaagaggtgc tggacaaaac
    10501 cagcggtaaa tttgccgagc agaaaaacat ctatcagcaa ctgtggtgtt tgccgaaagt
    10561 ggacggtaaa tacattcagg tatgtacctt caccgttggc ggcaactacg gtgggacgtg
    10621 tttgcgcggt gatgaatcac tggtcatcaa aaaagagagt gatattgaac cgttaattgt
    10681 ggtgaaaaag taatacatgt tgtagcaacc ccgtactgat tatgaagaat aattagtacg
    10741 gggattaatc aaaatattca atatttatat attccaatat ttattcattt catatgtgaa
    10801 tatattaacc agtggaatac ctgtgttttg attatttcta aaggttttga atgaatgctt
    10861 attgtctgat acacaagaaa taacactctt tttatcgtta aaaaatgata tttcactttg
    10921 cccatgccgt taaagtcact gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta
    10981 atatatgcta aatttatttg ttggccttga tatatatacg gggcttttgt tattacttgc
    11041 tctggcattt gtgttgttct acgaagcaat caatggtttt catgacacgg cgaatgcggt
    11101 ggcaaccgtt atttatactc gtgccatgca gccacaactt gctgtggtga tggcggcatt
    11161 ttttaacttt tttggcgtgt tattgggcgg acttagcgtt gcctatgcca ttgtccatat
    11221 gttaccaacc gatttgttgc tgaatatggg gtcaactcac ggtctggcga tggtcttttc
    11281 catgctgctg gcggcgatta tctggaacct gggaacgtgg ttctttggtt taccggcctc
    11341 cagttcgcac accttgattg gtgcgattat cggcatcggt ttaaccaacg cgctgttaac
    11401 cggctcatcg gtgatggatg cgttaaacct gcgtgaagta accaaaattt tctcctcgct
    11461 gattgtttcc cctatcgtcg gcctggtcat tgcgggtggt ctgattttcc tgctgcgcag
    11521 tttctggagt gggacgaaaa agcgtgaccg tattcaccgc attccggaag atcgcaaaaa
    11581 gaaaaagggc aaacgtaaac cgccattctg gacgcgtatt gcgctgattg tttccgctgc
    11641 gggcgtggcg ttttcgcacg gcgcgaacga cgggcagaaa gggatcggcc tggtaatgct
    11701 ggtactggtg gggattgccc ctgctggctt cgtcgtcaat atgaatgcgt ccggctatga
    11761 aattacccgt acccgcgatg ccgttaccaa cttcgaacac tacctgcaac agcatcctga
    11821 actgccgcag aagttgattg cgatggaacc tccattgcct gcagcatcga ctgatggcac
    11881 gcaagtaaca gagtttcact gtcatccggc aaataccttt gatgcgattg cgcgcgttaa
    11941 aacgatgctg ccaggcaata tggaaagtta cgagccgtta agcgtgagtc agcgcagcca
    12001 gctgcgccgc attatgctgt gcatctctga tacctccgcg aagctggcga aactaccagg
    12061 cgtcagtaaa gaagaccaga acctgctgaa aaaactgcgc agcgatatgt taagcaccat
    12121 tgagtacgct ccggtgtgga tcatcatggc ggtagcactg gcgctcggca ttggcaccat
    12181 gattggctgg cgtcgtgtag cgatgaccat cggtgagaag attggtaagc gcggcatgac
    12241 gtatgcgcaa ggcatggcgg cacaaatgac ggcggcagtg tctatcggcc ttgccagtta
    12301 tattgggatg cctgtctcca caacacacgt cctctcgtct gcggtagcag ggacgatggt
    12361 ggtggacggc ggtgggttac agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt
    12421 tactttaccg gcggcaattt ttctttctgg tgggctgtac tggatagcat tgcagttgat
    12481 ttaaccttcc tgaaaatgcc cggtcctggc gatcgggcat ttccatttt
//
LOCUS       DADLSE010000109        12425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLSE010000109 DADLSE010000000
VERSION     DADLSE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.109"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN692_003017"
     CDS             complement(74..4534)
                     /gene="mukB"
                     /locus_tag="JN692_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7265272.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADRGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN692_003018"
     CDS             complement(4534..5238)
                     /gene="mukE"
                     /locus_tag="JN692_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7265273.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN692_003019"
     CDS             complement(5219..6541)
                     /gene="mukF"
                     /locus_tag="JN692_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7265274.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN692_003020"
     CDS             complement(6538..7323)
                     /gene="cmoM"
                     /locus_tag="JN692_003020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7265275.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRLGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7459..8238
                     /gene="elyC"
                     /locus_tag="JN692_003021"
     CDS             7459..8238
                     /gene="elyC"
                     /locus_tag="JN692_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7265276.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8215..9108)
                     /locus_tag="JN692_003022"
     CDS             complement(8215..9108)
                     /locus_tag="JN692_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7265277.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN692_003023"
     CDS             complement(9262..10008)
                     /gene="kdsB"
                     /locus_tag="JN692_003023"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7265278.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEIR"
     gene            complement(10005..10187)
                     /locus_tag="JN692_003024"
     CDS             complement(10005..10187)
                     /locus_tag="JN692_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7265279.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10239..11471)
                     /locus_tag="JN692_003025"
     CDS             complement(10239..11471)
                     /locus_tag="JN692_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7265280.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN692_003026"
     CDS             complement(11508..>12425)
                     /gene="lpxK"
                     /locus_tag="JN692_003026"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7265281.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2865 a         3537 c         3085 g         2938 t
ORIGIN      
        1 ttatgtgcca tttttttagt gcagaaacgg agttttcgga aaaagaaaag gcggcattgc
       61 tgccgcctta attttaactc gcctgggaag gcgcttcgtc acttcctgga agcgtttcag
      121 ggagttgcgg cgcaaatcct cgcaggccga cgacgtgaac atgttcggta ttctggaaga
      181 ctttacgtac cagtttatag gtggtgcctt tttccggact gatattttcc ggcgctgcga
      241 tgatgagctg catttgcaaa cgttcacaca attcaaacag cgtggcgata gaacgagcat
      301 ccagtcgcgc tgcttcatcg aggaacagca ggcggcaagg agagatatct ttaccgcgca
      361 ggcggcgaga ttcatcttcc cagctttgta ccaccatcac cagaatcgac ataccggtac
      421 caatcgcctc accggtcgac aatgcaccag actctgcgcg caaccaacca tcggaaccac
      481 ggttaacctc aacttccatt tccagatagt tgcggtaatc cagcagttct tcgccaatgg
      541 tctgcggcgt gcgctgcccc atatcaatct gcggattaag acgctgatac aatttcgcca
      601 acgcttccga gaaggtcaga cggttgctgt taaacagatc ctgatgctgc tcgtgctgtt
      661 cagagagcac atccagcagc atggcgtgcg tttcacgcac gttcacgttg agacgcacgc
      721 tgttcacctg accaaacgat acgttctgca acccctggtt gagcatacgg atacggttct
      781 gctcgcgctg aatggttttg cgaatgatgt tcgccacgct gcgggaactg atcgccagtt
      841 tctgttcacg ggaggttaat tcttcggtca gacggctaag ttcaatctcc atctgttcga
      901 tagcttccac cggatcatcg gtacgaataa tatcctgacg aatacgttca cgcagatgct
      961 gataaaccgc cacgaagaac tgaattttac gctccggacg tttcggatct tccgacatgc
     1021 gcagcacgtc gcgcagatgt tcgttatccg ccaccgccag acgcagcgca cctaacgcct
     1081 tatccgacat ggaacgcaaa tcatcagcgg agagataagc cagctcacga cggtgtaagc
     1141 ggcgctcaac gccgttatct ttcaccatgc gcatcaccgc acaccagccc gctttggcgg
     1201 tcactacctg ctcgcgcatc tcaaagtaat cccgctccag cttgcgcagt ttgcgggtca
     1261 ggttgtccat ctccgcttcg cagaaggtaa gcgctttttc cagttgattg cggcgtgaac
     1321 ggttattgct cagttgcgca tgcagctcgt cacggcgaat acgcgcccgc tcttctgccc
     1381 ctctatcagc acgcacgccg atatcctgca attcacgttg cagatcgttg agtagctctt
     1441 ttttggtgtc gtaagaactt ttcagcgaag ccagcacctg gttgtactga ctcaactgcg
     1501 ctgcgtgacc gcgcaacgct tcgcgagcgc gggtacgctc cgcttccgcc tgttccagac
     1561 gttcacgcag tttttcgttg agatcgctgt taccgctaag catttctgcc gagtcagaat
     1621 agctaaagtg cgcacgacgc tgcaccactt ccgtcagggc aaacgcttgc tgacgggcat
     1681 cgcgctgcat ctgctgagag tacgcgtaat cttcttttaa ctgttcgaac tgttccgggt
     1741 cgctctgcaa taccgaaacg atcggttcca gtttcgccag ttgattgcca aactgctgaa
     1801 caaaacgcgc ggcttcctgg gcttcatcca gacgttcgcg gatttcatcg acgcgatccg
     1861 ccaggctgtc atcagccaac aggttgagac gcggcagaat gcggttcagc gccgtaacgc
     1921 cttctttcgc ctgctcaaac tgaatacgct gctgctggtt atcattttca tgattactca
     1981 acgcccgctc cagttcgact cgacggctgt tcagttgacg gatttctgct tccgggtcag
     2041 actcaaacgc caccgccaga tgactgccga taaagcggct gaacgcctga tgcagacgct
     2101 gggttttctg tacatcaaag gagagcgtgg cgaagcgttc ggaaagcact tcacgctcgg
     2161 catggaggct ttcaatacgg ctttcacgcg cagcacgacc aaacagcggc acttccggga
     2221 aacgtgaata acgccactgt cgatcggcga ttttcaccac caccgctttt tccagctcat
     2281 caacgctgaa cacgctatca tcgaatgact gcggatcccc ttcgatcaaa tagagatctt
     2341 ccgggcaatc ggtcaagcct tccaggtgtt cggttacctg tgacagatct ggcaccacga
     2401 tggcgtggcg tgacgggcca tacagagccg agaagtacgg cgcatcttcc aggctaacgt
     2461 cgtcataaat ttctgacagc agcacaccac caaaacgctc cgccagcgcg ttcagacgct
     2521 gatcttcaga gccgccaggc tggcttaaac gttcgatctc ttcatcgacg gcgtttttgc
     2581 gcgcgcctac ttcatcgcgt tcaacaatcg cctcgcgctc acgctccagc aactgttgca
     2641 gatattcagt gacgtcctgg ctggaggaga actcttcgcc gcactgttca ctcaactggt
     2701 tgagactgtt ttgcgctgcc agccaaaccg gtgcacgctg catcaaactc tgaatgcgag
     2761 actgcagctg ttcctgttcc tgccgcagtg ccatccgctc ttcacgggcg ttagatacgc
     2821 tatcggaaag agaggcaatg cgtgcttcca gctcctgatg cagagcttcc agttcatcaa
     2881 tatcaaaatt cttgccctga cgtttgcaga aatctgccag cagacgctca gcttcttgct
     2941 gctcacgcag acgctgttcc agttcgctta agcgcatccg caacggctga acctgctctg
     3001 ccaggtggcg ctgatcaacc ccttcgcgca atagttcgcg agcgacatcc cacgcctcgt
     3061 tacgcgccag cgggccgttg attgccacca ccagctgata agcctgctca aactggctgt
     3121 gcgcggtttg cgccatgctc attttctgct caagagagag cattttttca gtcgcttcca
     3181 gctctttcgc ctggaaggtt tccagccatt cggcggcgct gtcggcggtt aagtccggca
     3241 gatggcacag ttctttggca cgattaagcg cagcaatcgc ctggttatac tggatcgcgc
     3301 gcgtttgctg gacgtccagc gcctgctggt agtcagcaag ctggcttttc agctcgtcca
     3361 cttccagttc ggcagcttcc gcgcgagcct cattctcttc ctggcgttcg atggcttctg
     3421 ccaccacttc attttgctct tccagacgga tctgcagctc atcgagatcc acttcgtagc
     3481 gttcgatctt ctcctgctga cgcaatgcgg tttgcaccag gttcaggtga tcgctggccg
     3541 cctgataatc cgcttccaga tcgccttcgg caccgttgtg ctctgccagc tcacgcgcca
     3601 tatcgacgtg tttgtactgc tcagccgcca gttgctgacg cgaagtatgt agctcgcgac
     3661 gaaactccag ggctttgtcg agatggacac ggcgctcgtt ggcgtgacgc atgtagtccg
     3721 ccgccacgta gttggtagct tcgctgatca gatgcttaaa caggtcacgg tccgactggg
     3781 tgacacgaat cgcttccagc gtcatgcggt tttcacgcag cgccgcttcc atgtcctgga
     3841 acgctttacg cacgccgctg ttttccggca acaggtagtc gcgcagagaa cgggtaatgg
     3901 cactggagat cccgccatac agcgaagctt cgatcagacg atagaatttg ctacggtcag
     3961 atgcggagcg cagacgacgc gcgatgatgc ccagatcgaa catcagcgag tggtaatcgg
     4021 taatggagtt gaactgttta aactgcacgc cttccatcgc ctcgagcttg tctttcagct
     4081 cgttaagcgg cagcacgcgc gcctggcgtt cgttcagggt ttcggtcacc agctgtgtcg
     4141 gctgcaccga catcggcagt ccctgaatgg caaatggttt gatatccact ttacgatcgc
     4201 gtccggcaac ctgttgcaga cgcacaccga ccaccacgcg ctggtggcgc gagttaatgg
     4261 tgtcgagcat cgaataacag acacccgctt tcagcttacc gtgcagacct ttatcgcgcg
     4321 aaccgctggt ggccccggct tccgtagtgt tacggaaatg cagcagggtc aggtcgggga
     4381 tcagcgccgt aacgaacgcc gccatcgtgg tggatttacc cgccccgtta ccgccggaaa
     4441 gcgtcgtgac cagctcgtca aggtcaaaag ttcgggcaaa aaagccgttc cagttaatca
     4501 gcgtcagtga gcgaaattta ccgcgttcaa tcattattct tcctctccgc tatctggctg
     4561 actctcttcg gtttcatcgt tgagttgcag atgattttca atcggcattg cttcgccatc
     4621 acgaatcagg cgacgctgtg cttcacgggg atcgtcgcca gcacgcacat cggctccgaa
     4681 gcggaacacc gattcggtaa tgcggaactt gctgctgtcg tggcccataa accacaccat
     4741 gcctaaacga cgcagacggt tgagcgaaga acgtactttc tcctgcaact tctgacggtc
     4801 aacgtctgaa ccggttgaac ggttgttcac cagtttcagc agtttcgctt catcggccag
     4861 ggtgagcagt tcgtcgtaca gttcctgctg ggtgaaaatc ccctcattcg ccagccgttc
     4921 cgggctgaga tagagataac agaggatttt cccgaccatc atatccagtt ccgacaagac
     4981 ggaacgcggg ataagagtgg tggaacgtgg gcgtaaatag aagaaccctt ccggtgcgcg
     5041 aataagctca acgttataac gcgcgtaaaa ctcttccaga tattcctgaa aatccatcag
     5101 gaatgcatga ttatccagtt cgtcgaggcc aatatggcgg cctgaacgta aggcgctgtc
     5161 cagcgccgga aataacggat tcgccagcgc ctgcgccagc ttaaccggca tcacttgttc
     5221 aatatttgtc gatgacatgc gcctgtacct tggctccgta atcattaatc ggctgccatt
     5281 tcgctggcag tccggtgaaa tctgcttgcg ctacgccaag acgtaccgcc tgatcaataa
     5341 caatacgcgc aacgtcaaag tgacgtgcac gcggatactg tgacagatat tcgcgtacca
     5401 ccagaccaag atccagcggc acttgtctgg ttttgtacac ggcaagttgt tcttcgatga
     5461 tcgccgccag ctgttcgcgg atctcgttaa actcttcgta ttccagatcc tcaggaagtt
     5521 ccccagtcac ttcttcatcg cgcagtgcca tctcttcgtc acgcatatcc agcagacgat
     5581 cggcattggc ataagttagc gcccacggct catcaaaata ggtttgtacc gactgacgta
     5641 accgctgagc aaagacgcgg tttttatcca tatcgatcgc ggtacgaata aatttgtgta
     5701 cgtggcggtc gtagccaatc cacaagtcga tggattgctg gccccaactg ataatacgat
     5761 cgagtttgct ctgcagatcg aacaccagac gatcgacgaa atgcagatcg tcatgggtca
     5821 tcgtcgcatc ctgaatgcgc aacagattag cctgcaattt gtcgcctgcc gcttccagcg
     5881 tatcctgcaa ttcacgcagc gttccggaag tttccgaaag caacaattca cagctggaaa
     5941 tcgccgcccg ccagtctttg ttcagcaact gggcgatatc gtccttcacc tgctgctgct
     6001 gttcgtccat cagacgttgc gtcaggtcga tactgtcgaa aatttctgct accgaatatt
     6061 tcagtggcgc atagacatta cggtgccagt gaaattcatc accgccctct tcggcggcat
     6121 ctgctgcgcg tttgagctca cccgccacaa tcgacaactg catagaaaga cgcagcgtag
     6181 aaaactcgcg ctgacggatg tagtagtcag taatgccgat gccgagcggc gtcagacggt
     6241 aaattgcgtt cccttccgcc tgctcgctgg taaagcggtt cagcagacgt tgacgcacca
     6301 tgtcgttgat cgcgttattg gcgcgcacgc cgatggtttc gctggtttgc tcaaacgcat
     6361 cactcacatg gcggaatgca tccaccagct cgccttcact catctcacca tccagacgct
     6421 cgccgttcag cgtggcaacc gccagcagaa aagagagtcg gtctaccggc agcgagatgg
     6481 agaagtcatt ttttctggcc caggcaacca gttcggggac tgtctgggaa aattcactca
     6541 tactttatcc ttgctctgcg gtttgcgcgc ggtgacatga atataacgcc ctagggtaat
     6601 atacggttcc tgacggcaat aacgcgtttc taattcaagt aatgcttcat agcagtcgcg
     6661 ctgctggtgc ttctcgcgca gataatcatg aaacacgcga acgcctgtct tacccataat
     6721 ttgccaacca gcttcttcca gccacagata aacctgcgcc gggtcgcgtg gataatctgg
     6781 cgaaagcgtc cgttttttct ttttcggcat tcccgcctgc acgtaatcaa aattcccggc
     6841 gaccatgtta tgcatcaaca aaccatgcgc attgtagaac attaacgaca acactccgcc
     6901 tagacgcaac actgaccaca gggtctgcaa tacactgcgg ggatcggcca cccactcgag
     6961 caccgcatgg aacaatatca gatcaacggg cgtttccaaa tgcgaagcaa catcctgagc
     7021 ggcgcaatgt ataaattgca tgttgtcgct cacacctttt gcttctgccg cctgttttgc
     7081 gcggtcgatc atctgcgcag aaagatcgca taaaataacc tgatgcccgc gctcggccat
     7141 tttgattgcg gtctgccctt ctccgccgcc agcatccagc acacgcagtt tttgcgggcc
     7201 catttccgcc agcacgcgat cgagatcctg ccacagaata gcctgtcgaa gctgcccttt
     7261 ggtggtgccg taaatgttac gggaaaactt ttccgcaata tcatcaaaat tgcgatcctg
     7321 cattgctttc tccaccggtt ggctaagacg ttttctgata aagacgctat tttgtcacag
     7381 ccacggcgag aatgaacccg tctggtgtaa tatcgctggc gatcccttgc tatatggtta
     7441 aaaaaggaac cagaaaggat gctttttaca ctgaaaaaag tgattggcaa tatgttgctg
     7501 ccacttccgc tgatgttgtt aattattggc gctggcctgg cgctgctgtg gtttagccgt
     7561 tttcaaaaaa ccgggaagat ttttattagc attgggtggc tggcactctt gttattgagt
     7621 ttgcaaccgg tagccgaccg cctgctgcgt cccatcgaaa gcacctatcc gacatggaat
     7681 aattcgcaaa aagtggacta tatcgtggtg ctcggaggtg gttatacctg gaacccgcag
     7741 tgggcaccga gctctaattt aatcaataac agtctgccac gcctgaatga gggtatccgt
     7801 ttatggcgcg aaaatccggg ttcaaaactg atcttcacgg gaggcgtagc aaaaaccaat
     7861 acggtgagta cagcggaagt aggtgccaga gttgcgcaat cgctgggcgt accgcgcgag
     7921 caaattatca ccctggattt gccaaaagat accgaagaag aagctgcggc agtgaaacag
     7981 gcgattggtg acgccccttt cctgctggtg acctccgcat cacatctgcc gcgggcgatg
     8041 atcttttttc agcaggaagg gttaaatccg ctaccagccc cggcgaatca gttggccatc
     8101 gactcgccgc ttaatccgtg ggaacgggca atcccttctc cggtctggct gatgcatagc
     8161 gatcgtgttg gctatgaaac gcttggccgc atctggcagt ggctgaaagg tccgtcaggc
     8221 gagccacggc aagagtgatt ttgatgccag atcgaagttg cgccgactaa atcgtccggt
     8281 attaaccagt tgcgcaactt catcccataa cacatacaac caacgccgcc agatgaacga
     8341 ctccgccact ggcgcgcgtt gcaggtaact ccagagcaaa tcttccgcca gagaattatc
     8401 cattagtcgg aacagttcgt attctcgcgg tgcccaaagc attagtcccg gcccgaccat
     8461 cgccagtaac tgatcgctgc gcgaatcttt cagcatgctg cgtaaacaga agttaccgtg
     8521 aatcaacaca caattgtcgt taaagccttc aaataatgcc gggagacatt cgcgagtgcg
     8581 aaacaggatc cgcttatcct gcatcgtcag accggtgtta ttgaactgat tgagcgtggt
     8641 ccatagcact tcaacatgtt gccggtacca tgagggccag aaattttcct gagtattgtc
     8701 gaccgcgccg acgcaaccgc gactgtcctg acggtgccag gccagtaagg cttcaacgat
     8761 ttggtctttg agctgttccc agcgttctgg tgttcgggct ggtgcctcca ccgaaacacc
     8821 acgcatccgc tccagtagca ggacatcagg gccggggtgt tcttcatgcg tcatcacacc
     8881 ataaatagtc ggcatacgga cagtcccact tcttgccagc atggtggttt tccatgccag
     8941 ttgtcgggct tttccgggcg tactaaagct tcttgccatt aacggcattg ggtttccctg
     9001 gctgtcatac aaagcccaca acgccgtatc cgctttttca ttgacgcact caatacgact
     9061 gagtttttcg cccagtaaat ggcttaattc ggcacgcagc tgttccattc agattaccct
     9121 cataaaagct actgctttca taatgagcgc caatataaaa gatgtcacct gaatgagatc
     9181 aattgttgaa aagaaaaatt gaggaaaatc gtaacggtac gacactcctc ccaaaattgg
     9241 ctgaagtgtc gtgaagtgaa attagcgtat ttcagcgcga acgcgctcaa gatcttcagg
     9301 ggtatccaca cctgtgccag gaacttcctg agcaacagca acatggattt tttcgccgta
     9361 ccacagaaca cgaagctgct ctaacatttc gatgtgttct aacggacttg cctgccagtt
     9421 gacgtaacga cggataaagc ctgcacggta gccataaata ccaagatgac gcaggaagtt
     9481 atcgccaacg gtttcaaggc cttctgcaaa acgatcacga tcccaaggaa tggtggcacg
     9541 tgagaagtac agtgcatacc cttcagcgtc gagaaccact ttcaccgcat tcgggttaaa
     9601 cgcttcttcc gcattgtgga ttggcaccgc cagagtcgcc atacccacct gacgctgagc
     9661 gaggttatca gcaacctgac gaatgattgt cgcagggatc atcggttcat caccctgcac
     9721 attaacgatc accgtgtcgt cgctgaatgc gcatttttcg acaacttccg ccagacgttc
     9781 tgttcctgac tgatgatcgg cgcgcgtcat acatacttca ccgccagcgg cttcaacggc
     9841 gcgggcaaca tcctcatgat cggttgccac gatgatgcgc tcggcacctg attcacgcgc
     9901 gcgttcaaga acatgaacaa tcatgggttt gccgttaata tcaaccaatg gtttaccggg
     9961 cagacgcgtg gacgcgtagc gcgcgggaat aatgaccaca aaactcatga tttactctca
    10021 tcagcagtca gcacgcgggc ttccgtttcc agcaacactg gaatgccatc gcggagcggg
    10081 aaagccaggt tatccagttt gcaaataagc tcttgttttt cctggttata ccaaagtttt
    10141 ccgttgcaaa ccgggcaggc aatgatttca agcaaacgat gatccatagt tcctccagat
    10201 ggatcgggtt atgaatgcat aaatcttatc ataatcattt atgcgacggg gtctatttcc
    10261 cagccagtgc ggcaatcagt aaagagacct tgcgggcagc gtcctaatgt cacccgcgtt
    10321 gcctgctgcc agctcgcgaa atcagtaatc gcctgacgta accctttttg cagcattgtc
    10381 gttggtttaa tgccttcctg taaccacaga gagataactt caaggatgcc tgtctggcga
    10441 tgcattttgg catccattcg cccaactaat tgcccacgat gtaataatgg cagaacaaaa
    10501 tagccatact ggcgtttcgg cgctggggta tagcactcca gccggtagct aaaatcaaaa
    10561 agctgctctg cgcgtttgcg atcccagaca acaggatcaa aaggcgaaag taccgcgctg
    10621 tgcgttgcag tgagctttcc ggccagcgca cgctcgagta gcggcagcaa atcagcatgc
    10681 agccaaagat tgcccaattt ttcaacatgc acagcaatga tttgctgctg ttcagccctc
    10741 gcttcgcgcc acgccgccag tgccggacgt ttcagccgat agtaatctgc cagccactgt
    10801 tcgcggaata ttcccaggct acgcgcactg ttatccagca tgatgatttc tgcttctgtt
    10861 tgcgaaacga gatcgcgctc atcatcccag tcaggcatga cacggtgggt taaatcataa
    10921 acgcgctgga agttgcgccg ttcaatcacc atcacctttc cggcagtaaa taaaccttcc
    10981 agatgccgtt tatgcggctt ccattcccac cagccgcttg cacctttacg aggatgctca
    11041 aaatcggctg aacgtaccgg ccctttatca tgaatatgct gaattaactg tgcaatttcc
    11101 gcctcatgtt cctgcatcca ggcgtctttg tatttccagc ccattttttc aggtgccagc
    11161 atgcggtggc gaataagacg aaagtcgcta cgcggcataa agcaggcttc atgcgcccag
    11221 tattccatta attcgccacg cgccagagac tcatccagcc actgggcagg ataatttccc
    11281 agacgactga aaagcaccag atatggacta cgggcaacaa tattgatggt atcgatttgc
    11341 agcaaggaca tgcgggagat cgttgccgga atatcctcca acgacgctcg acggcggggt
    11401 ttgtttaaca ggccttgtgc ggcaaggtga agattacgcg catcagcaag ggagaggtgc
    11461 ggcagcgaca ttcatgactc catcaatcga acgctgccgc ggcgtaacta gttgccagaa
    11521 gccagcgagg ttagttgcgt aagcagtttc gctggttcat cacctgaaag ctgtgcgtct
    11581 acaggcaaat accaccaatt ttcttctgca aaggcccggc atttcaccgc atctttttca
    11641 gtcattacca gcgtttgccc ggcgcttacc aacgcactga catccgcatg gttcaaagac
    11701 tgatgatcgg ccagcggtac acatttttcc ggttgtacgc cacacatctt cagcgtggca
    11761 aaaaagcgcg gcggatgccc aatccccgcc atcgccacta catgttcaag ctgagcaacg
    11821 tcacaacgcg taccggtacg taaattcacc gcctgacccg gcagcagatg catggggatt
    11881 tcaccgctgc gagggacacc gccgttgacg attaccgcat caaccgactt taagcgcccc
    11941 gctcgctcac gcattggccc cgccggcaac caccagccat tgccaaagcg acgcacacca
    12001 tcaataacga caatttccac atcacgcgcc agacggtaat gctgtaaacc gtcgtcggtt
    12061 acgatgatct gcacatcagg gtgttgcgcc agaatggctt ttaccgcatc agaacgaacg
    12121 ggagaaaccg caacaggcgc atcagtgcgt tgataaatca acacaggttc atcacccgcc
    12181 tgtgctgtgg tggtatctgc cgacaataac agcggataag attcagcctt accaccatat
    12241 ccccgcgata cgacccccac gcgaataccg cgctgttgca actgttccac cagccagaca
    12301 acgaccgggg tttttccgtt gccgcctgcg gtgagattac caaccacgac aaccggtacg
    12361 ggggcacgcc aggcgcgctt cagttttagt ttatagcaaa gacggatcgc gccactcacc
    12421 aggcc
//
LOCUS       DADLSE010000110        11972 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLSE010000110 DADLSE010000000
VERSION     DADLSE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.110"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..177
                     /locus_tag="JN692_003027"
     CDS             <1..177
                     /locus_tag="JN692_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7265282.1"
                     /translation="QTERLLTWRGMYQQLLKVDPLKCELCGGQMRFTGLKMGYRLAEQ
                     IMMHEPLARMRWCG"
     gene            326..611
                     /locus_tag="JN692_003028"
                     /pseudo
     CDS             326..611
                     /locus_tag="JN692_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1013..1549
                     /locus_tag="JN692_003029"
     CDS             1013..1549
                     /locus_tag="JN692_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7265283.1"
                     /translation="MKYIVFVSALLISNTGKGGGQRVVFNVDIVGSACHVVVDADGFG
                     NSGRLIFGTYRKSSGVPVLPRDFVVRLYESGATVQGCSAFRAGQVATLDFGNPGQLDA
                     KGVVTRGAGDGIRVDVRAVDTQADYRGRLTQDSHSVNYPVEFAVKGQFRFRAQPVFPA
                     NVKAGEYTGALTFVVTYQ"
     gene            1564..4002
                     /gene="faeD"
                     /locus_tag="JN692_003030"
     CDS             1564..4002
                     /gene="faeD"
                     /locus_tag="JN692_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial usher FaeD"
                     /protein_id="HAY7265284.1"
                     /translation="MCAGKKVVAELVSSLFSRRAALAVSAAFASTTGVAGEKLDMSFI
                     RGGAGVNPEVWAALNGSYAPGRYLVDLSLNGKEAGKQILDVTPQDSDELCLTEAWLAK
                     AGIYVSADYFREGYDATRQCYVLTKAPSVKVDFDVSTQSLALSIPQKGLVKMPENVDW
                     DYGTGAFRVNYNANANIGRNNTSTFGSADLKANIGHWVVNSSASASGGDSGDNSATIN
                     AFTATRAIRALSADLTVGKTSTGSSLLGSTGTYGVSLSRNNSMKPGNLGYTPVFSGIA
                     NGASRVTLTQNGRLLHSEMVPAGPFSITDVPLYTSGDVTMKITGEDGREQTQLFPLSV
                     MAGQLNPGQHEFSVAVGLSDDDSDLKGGVFAASYGYGLDGLTLRTGGVFNRDWQGISA
                     GAVLGLSYLGAVSADGAYAMAEYRDGSRSGNKVQLSWSKQLEMTNTGLRVSWSRQSEE
                     YEDMSSFDPTELWSQSNHGRRPKDEWNAGISQSVGGLFSLSVSGWQRSYYPAQMTGRY
                     RYSDDNGLDTGITGTLSTQIKGVSLNLGWSGSRNSQGENNRSASASISVPFTLFERRY
                     SSSTSVSTSKGGGTGFSTGVSGSLNDRFSYSLGGGRDSGGGVSSYLNASYSGDRAYLN
                     GALNHSQSGGTSGSVSVSGSVLVVPMAKDIMFSRTTGDTVAVVNVKDTPGVKVTSGDG
                     QTDSDGNLVVPLNSYDWNTVTIDAGTLPLSTELTNTSQKVVPTDKAVVWMPFDALKVK
                     RYLLQVKQRDGEFVPGGTWARDSKNTPLGFVANNGVLMINTVDAPGDITLGQCRIPAA
                     RLQDTEKLQEITCE"
     gene            3995..4777
                     /gene="faeE"
                     /locus_tag="JN692_003031"
     CDS             3995..4777
                     /gene="faeE"
                     /locus_tag="JN692_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbrial chaperone FaeE"
                     /protein_id="HAY7265285.1"
                     /translation="MSKRNAVTTFFTNQVTKALGMTLALMMSCQSAVASLAADQTRYI
                     FRGDKDALTITVTNNDKERTFGGQAWVDNIVEKDTRPTFVVTPSFFKVKPNGQQTLRI
                     IMASDHLPKDKESVYWLNLQDIPPALEGSGIAVALRTKLKLFYRPKALLEGRKGAEEG
                     ISLQSRPDGRTMLVNTTPYIFAIGSLLDGNGKKIAADNETAQKLLMFMPGDEVQVKGN
                     VVKVDSLNDYGELQTWTINQKKSAISSAQKSSDTEGNSSDKK"
     gene            4812..5303
                     /gene="faeF"
                     /locus_tag="JN692_003032"
     CDS             4812..5303
                     /gene="faeF"
                     /locus_tag="JN692_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeF"
                     /protein_id="HAY7265286.1"
                     /translation="MKKTMMVVALALSALSIQSTLAAEYSEKAQYLGVVNGQVVGNSV
                     VKVTRIPTDPVLYRSGDTTPLPDRLTIRNAESRAASGGLAYITVKQVLPDNGEARITL
                     KTALMVDGKKVAISARQQGEDMVITLPEAQKQIELRTDAPAELEVPVSYRGNLQIALQ
                     VED"
     gene            5541..6341
                     /locus_tag="JN692_003033"
     CDS             5541..6341
                     /locus_tag="JN692_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cshE pilin"
                     /protein_id="HAY7265287.1"
                     /translation="MKKTLIALTVAASAAVSGSAMAWNPSGLGGNGGTFSMSGTLSKA
                     EKTIPWAVEIGAPVTALDVTVKPDQKNVFILLEKDIPILGIRSNPTGFMGEKGIAPQI
                     DYNKAADVDYMDKGNLPAQVNVTSSDGKKIGTLAFVMRVACQASEKRGEGDDKLESNM
                     LFASSKGKAFFGGVAKDDKGVWSDAAAYTWATHLFSSIANSWDTKIPYTAGDTDETDF
                     SNGDRLYRGYYASGLAKDIPMELVLDQTITSATPVPWKASLPIVISYM"
     gene            6562..7359
                     /gene="faeH"
                     /locus_tag="JN692_003034"
     CDS             6562..7359
                     /gene="faeH"
                     /locus_tag="JN692_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeH"
                     /protein_id="HAY7265288.1"
                     /translation="MKITHHYKFFLSAVILVTIFYSAVPYANILDGGEIQFNGYVTDE
                     APKWAWQISSPDQTWSVDTADARTENGQLVFNLHDKGALPFLEGHLHDVAERGGPGFT
                     PFITFSSNGQPFTVTEGNGASAQHFRASVPVRDPETGNVSGQLFFTLNQGMAVSTGRQ
                     DDGASVPAGMSLVSGQSVTDVQPGSLPQGLKARLSSLLLMNQNFGNGMNAVDNGQVIN
                     QGILTDGRVMDLAAAYASEVSDFELRLPAEGTPAAWQAGLNVTVTVQ"
     gene            7389..8141
                     /gene="faeI"
                     /locus_tag="JN692_003035"
     CDS             7389..8141
                     /gene="faeI"
                     /locus_tag="JN692_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F4 (K88) fimbria minor subunit FaeI"
                     /protein_id="HAY7265289.1"
                     /translation="MKKVTLFLFAASHLPSCVLAWNTPGENFSGELKLEGPVTSTRNP
                     WVWKVGQGNGGLEVKQSGGGGGIQVALPAMTVLLGKTTLTTPAGREGLSPRVSYGKGT
                     EGFSLVWTAPGMAEVTLPVTGDNNARAGTFVFRIQAVGVLRHVQGGQPVYVGMYDDLN
                     TNGLPGESTAMKVSDIPGVLQKMFSGEGPGWLQTMTLSGYSGVSHFSDDFLRQVEGVY
                     GAQTVAGSGELRLNGEMPEHWRVSLSVNIEYQ"
     gene            8466..9692
                     /locus_tag="JN692_003036"
     CDS             8466..9692
                     /locus_tag="JN692_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7265290.1"
                     /translation="MSGTIPKIKTGENVLSAAIQRIEWLFGTFPSVCLSFSGGKDSTV
                     LFHLVSEVARKKRRRFSVLFIDWEAQYQCTITHIQKMQKMYLDVTDRFYWVALPLTTV
                     NGVSQFQPEWICWEPGVTWVRHPPEYAITDMAYFPFYRYAMTFEEFIPAFSSWFARDK
                     YLVAVLTGVRADESLNRFMGLVSRRKQRYADDKPWTTASPEGFYYTMYPLYDWKARDI
                     WIYNARTRAIYNPLYDLMHRACVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            9677..10315
                     /locus_tag="JN692_003037"
     CDS             9677..10315
                     /locus_tag="JN692_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7265291.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            10543..10935
                     /locus_tag="JN692_003038"
                     /pseudo
     CDS             10543..10935
                     /locus_tag="JN692_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10954..11427
                     /locus_tag="JN692_003039"
     CDS             10954..11427
                     /locus_tag="JN692_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7265292.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            11579..11841
                     /locus_tag="JN692_003040"
                     /pseudo
     CDS             11579..11841
                     /locus_tag="JN692_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904911.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         3036 a         2531 c         3382 g         3023 t
ORIGIN      
        1 cagacggagc gcttactcac atggagaggg atgtatcagc agttactgaa ggttgacccg
       61 ctgaagtgcg agctgtgcgg aggtcagatg cggtttacgg gactgaagat gggttaccgt
      121 ctggcagagc agattatgat gcatgagccg ctggcacgaa tgcggtggtg cggctgagcg
      181 ccgcagaggg gaagttgcgt ccatgttgag gggaacggag caaaaaacca tcagagatat
      241 attgaatcaa tcaatgtaag caatatcaag tgtctatacc aattacgtga tgatcaggtc
      301 gtgttgaata aacgcgcatt cttcggtgct tttctaatct tttggggatt taagttcctg
      361 tcgatgaact gctggcgctg cttccccctt ttaccatcgg tatgttgacc actgacgaat
      421 ggggtagtta caccagagag ttaccgaaag agaagtatct gactggtaag atttttactc
      481 agcgcattga gcgcaacaat ctgcccctga gaacccgaat caaacgcctg gctcgcagaa
      541 ccatctgttt ctctcgctct gtaaaatttc atgaaaaagt tatcggtgct tttatcgaaa
      601 aacatatgtt cctggagtca tcaccctttt cctcttggtg ttatcccatc aatatttcgg
      661 ccacgtggtt tacgtttatt attgataata atcaatgaac tgccgagtaa aaataactgc
      721 tgatatacgg gatcccatgt caactcccgc attatatatt atacctcaat agcgttacat
      781 tattaatgta tgtaaatgtt aattaaccaa agcattaatc aattgttata taattgcgtc
      841 gcaggtaaac gccaaatcaa cgcaaataca aatagtaaaa atggttcatg ttttggcact
      901 atttggtttt tttgttttta acgggtggat atttgtttat tttttttcta aattattttt
      961 atgtaatttt cataaaaatg gggtgtgagt tattaactct aaattagtga gtattaaata
     1021 tattgtgttt gtctctgctt tattaatttc caatacggga aaaggggggg ggcagcgagt
     1081 tgttttcaat gtggatattg taggttctgc ctgtcatgtg gtggtggatg cggacggttt
     1141 cggcaacagt ggtcgactga tattcgggac ttaccgtaaa tccagtggcg tgcctgtatt
     1201 gccacgtgac tttgttgtgc gcttgtatga gtccggcgct acggttcaag gttgttcagc
     1261 ctttcgagcg gggcaggtcg caactctgga ttttggtaac ccgggccaac tggatgcaaa
     1321 gggcgtggtc actcgcggtg ctggtgatgg tattcgcgtg gatgtacggg cggtggatac
     1381 acaggcggat tatcgcggac gtctgactca ggacagtcat tcggtgaatt acccggtgga
     1441 gtttgctgtt aagggacagt tccgttttcg tgcacagccg gtgttcccgg cgaacgtgaa
     1501 ggcaggggaa tacacagggg cgctgacctt tgtggtcacg tatcagtaac agaggaaagg
     1561 tgtgtgtgtg caggaaagaa ggtggttgca gagctggttt cgtctttgtt ttcccgtcgg
     1621 gctgcgcttg ctgtatcagc tgctttcgcc agtaccacag gtgttgcagg tgaaaaactg
     1681 gatatgtcct ttatccgggg gggtgccggg gttaatccgg aagtctgggc agcgctgaac
     1741 ggcagctatg cgccgggacg ttatctggtt gacctgtccc tgaacgggaa ggaggccggg
     1801 aaacagatac tggatgtgac accacaggac agtgatgaac tgtgcctgac agaggcatgg
     1861 ctggcgaagg ccgggattta cgtcagtgca gattactttc gtgagggata tgacgccaca
     1921 cggcagtgtt atgtgctgac aaaagcgccg tcagtgaagg tggattttga tgtttccacc
     1981 cagagtctgg cgctgtccat tccccaaaag gggctggtga agatgccgga gaacgtggac
     2041 tgggattacg ggactggtgc atttcgcgtg aactataacg cgaacgccaa catcggccgg
     2101 aataacacct cgacctttgg ctcggcagac ctgaaggcca atatcgggca ctgggtggtg
     2161 aattcttctg cctcagccag cggtggtgac agcggggata actccgccac gataaacgcg
     2221 ttcacggcca cccgggccat ccgcgcactg agtgcggacc tgacggtcgg gaaaacatcc
     2281 accgggagca gtctgctggg cagtacggga acgtacggag tgtcgctgag ccggaacaac
     2341 agcatgaagc cgggcaatct ggggtatacc ccggtgttca gcggcattgc gaacggggcg
     2401 tcgagggtga cactgacaca gaacgggcgg ttgctgcatt cggagatggt gccggcaggt
     2461 ccgttctcca tcacggatgt gccgctgtac accagtgggg atgtgaccat gaaaatcacc
     2521 ggcgaggacg gtcgtgagca gactcagctc ttcccgttgt cggtgatggc cgggcagtta
     2581 aacccgggac aacacgagtt cagcgtggca gtcggtttgt ctgacgatga cagtgacctg
     2641 aaaggcggcg tgtttgcggc gtcatacggt tacggtctgg atggactgac gctgcgcacc
     2701 gggggcgtgt ttaaccggga ctggcagggt attagcgccg gggctgttct ggggctgagt
     2761 tatctggggg cagtgtctgc tgacggggct tatgccatgg cggaataccg tgacggcagc
     2821 cgcagcggaa ataaggtgca gttgtcctgg agtaaacaac tggagatgac gaacaccgga
     2881 ctgcgggtga gctggtcacg gcagagtgag gaatatgagg acatgtcctc ctttgacccg
     2941 acagagctgt ggtcgcagtc aaatcatggt cgccggccga aggatgaatg gaatgccggt
     3001 atcagtcagt cggtgggggg gctgttcagt ctgtcggtgt ccggctggca gcggagttat
     3061 tacccggcac agatgactgg ccgttatcgg tacagcgatg acaatggtct ggacacaggg
     3121 attaccggta ccctaagcac acagattaag ggcgtcagtc tgaatctggg ctggtccggc
     3181 tcacggaatt cgcagggaga gaataacagg tcggcgtcag cctctatctc ggtgccattc
     3241 acgctgtttg agcgtcgcta cagcagcagt acatcggtga gtaccagtaa aggcggtggc
     3301 acgggcttca gcactggcgt gtccggctcg ctgaatgacc gtttcagcta tagcctgggc
     3361 ggcggtcgtg acagcggtgg tggtgtgagt agttatctaa acgcgtcgta cagtggtgac
     3421 cgggcttatc tgaatggtgc cctgaaccac tcacagtccg gcggaaccag tggttctgta
     3481 tcagtcagcg gttcggtact ggtcgtcccg atggcgaaag acattatgtt cagccgcacg
     3541 accggggaca ccgtggccgt ggtgaacgtg aaggatacac cgggtgtgaa ggtgacgtcc
     3601 ggtgacggac agactgacag tgacggcaac ctggtggtac cgctgaacag ctatgactgg
     3661 aacacggtga cgattgatgc gggcacactg ccgctgagca ccgaactgac gaacaccagt
     3721 cagaaggtgg tgccgacgga caaggcggtg gtctggatgc cgtttgatgc cctgaaagtt
     3781 aagcgttacc tgctgcaggt gaagcagcgt gacggtgagt ttgtgccagg gggaacctgg
     3841 gcacgtgaca gtaagaacac accgctgggc tttgtagcta acaatggtgt gctgatgatt
     3901 aacacggtgg atgcgccggg tgatatcacc ctaggccagt gccggatacc tgcggccaga
     3961 ctgcaggata ctgagaaact acaggagatt acgtgtgagt aagcgtaacg cagtaacgac
     4021 gtttttcact aaccaggtga caaaagcact gggaatgacg ctggcgctga tgatgagctg
     4081 tcagagtgcg gtggcttccc tggcggcaga ccagacccgc tatatctttc gcggggacaa
     4141 ggatgcgctg accatcacgg tcaccaacaa tgacaaggaa cgtacctttg gtggtcaggc
     4201 atgggtggac aatatcgtgg agaaagacac ccgtccgact tttgtggtga cgccgtcctt
     4261 cttcaaggtg aagccgaatg gtcagcagac actgcgtatc atcatggcct cggaccatct
     4321 gccgaaggat aaagagtcgg tgtactggct gaacctgcag gatattccgc cggctctgga
     4381 gggcagcggt attgcagtgg cgctgcgcac gaaactgaaa ctgttctatc gcccgaaggc
     4441 actgcttgaa ggtcgcaagg gggcagaaga aggcatcagt ctgcagagcc gcccggatgg
     4501 caggaccatg ctggtgaaca ccacgccata catttttgcg attggcagcc tgctggacgg
     4561 aaacggaaag aaaattgccg cggataacga gacggcgcag aaactgctga tgttcatgcc
     4621 gggtgatgaa gtacaggtga agggaaatgt ggtgaaagtg gactctctga atgattacgg
     4681 tgaactgcag acatggacga ttaaccagaa gaaatcagca atatcgtctg cacagaagtc
     4741 atctgataca gagggtaatt catctgataa aaaataatcg ccctccggca tacggaacag
     4801 aggggtaata aatgaagaaa acaatgatgg tagtcgcctt ggccctgagt gcactcagta
     4861 ttcagtcaac acttgccgct gaatacagcg agaaagcgca gtatctgggg gtggtgaatg
     4921 gccaggtggt gggtaacagc gtggtgaagg taacccgcat accgacagat ccggtgctgt
     4981 accggagtgg agacacgacg cctcttcccg accgcctgac catccgtaat gcagaatcca
     5041 gagcagcatc tggtggcttg gcgtatatca cggtgaaaca ggtgctgccg gataacgggg
     5101 aagcccgcat caccctgaag acggccctga tggttgacgg aaagaaagtg gcaatcagcg
     5161 ccagacagca gggtgaggat atggtgataa ctttgccgga agcacagaag cagattgaat
     5221 taagaacgga tgcaccggca gagctggaag taccggtcag ttaccgggga aatctgcaga
     5281 tagcgctgca ggtggaggac tgaggattca tttcctttat aaatgcagta catcccgcga
     5341 taccggagcg ggtcatattg gtaaagggat ttaccgtctt ttaaaacggg aataacgtaa
     5401 aactattctg agttaaacac tgtgtttaat gaaatgcggt tatttaaatg aatccgcagg
     5461 ggatggtttt gcggtaattc cgggaaaata aggattaccg attcagttta ttatttatga
     5521 aacgtcaagg tgattatttt atgaaaaaga ctctgattgc actgactgtg gctgcttctg
     5581 ccgcagtatc cggctctgct atggcgtgga atccctctgg ccttggtggc aacggtggca
     5641 cctttagcat gagtggtact ctgagcaaag ctgagaaaac aataccgtgg gcagtggaaa
     5701 taggtgctcc ggtaacagcg cttgatgtga ccgttaaacc tgaccagaag aatgttttca
     5761 ttcttttaga aaaagacata ccgatacttg gcattcgtag caacccgact ggtttcatgg
     5821 gggaaaaggg tattgcccca caaatcgatt ataataaagc cgctgatgtg gattatatgg
     5881 ataaggggaa tttacctgcg caagttaatg tcacatcatc tgatggtaag aaaattggta
     5941 ctctggcgtt cgtaatgcga gttgcctgtc aggccagcga aaagaggggt gaaggcgacg
     6001 acaagttaga gagtaacatg ctattcgcca gttcaaaagg taaagctttc tttggtggag
     6061 ttgccaaaga tgacaaaggt gtctggagtg acgccgctgc ctatacttgg gctacccacc
     6121 tttttagcag tattgctaac tcgtgggata ctaaaattcc ttacacggcg ggcgatacag
     6181 atgaaaccga cttcagtaat ggagacaggc tttaccgagg ttactatgca tccggccttg
     6241 ccaaggatat ccccatggaa ctagttcttg atcagactat aacgtctgct accccagttc
     6301 cgtggaaagc ttctctgcca attgttatct cctacatgta attcagcagt gttcagcagt
     6361 aagcacttac ccgtttactg ctgattaaaa cttcagaaca cagtttccgg tgacccgacc
     6421 tgaatataca gaaccggtat gtcagaacag gtgttttttc tgttggagtg tcttgtgacg
     6481 catattggtt ctgtgctttt tttaacaaaa ttattttatt gagctgtttt gttttatccg
     6541 gataaacagg aatgaatgcc gatgaaaata acgcatcatt ataaattctt cctttctgct
     6601 gttattttgg taacaatatt ttattcggca gtgccatatg caaatattct tgatggcggc
     6661 gaaattcagt ttaatggcta tgttactgat gaagcaccca aatgggcctg gcagataagt
     6721 tctcccgacc agacctggtc tgtggatact gccgatgccc gtacagagaa cgggcaactg
     6781 gtttttaatt tgcatgataa aggggctctg ccatttctgg agggacatct gcatgatgtg
     6841 gctgagcgcg gtggtcccgg attcacgcca tttattactt tcagcagtaa cggacagcct
     6901 tttactgtga cggaaggaaa tggtgcctca gcacagcact ttcgcgcctc tgtcccggtg
     6961 cgtgacccgg agacggggaa cgtgtcggga cagctttttt ttaccctgaa tcagggaatg
     7021 gcggtcagta ccggcagaca ggacgacggg gcatccgtac cggctggcat gtcgctggtc
     7081 agtggacaga gtgtgacgga tgtacagccg ggaagtctgc cacaggggct gaaagcccga
     7141 ctctcttccc tgttactgat gaatcagaat tttggcaatg gcatgaatgc ggtggataac
     7201 ggacaggtta tcaaccaggg gatactgact gacggtcggg tgatggatct ggctgcggca
     7261 tatgcctctg aggtgtcgga ttttgaactg cggttgccgg cagagggtac acctgcagca
     7321 tggcaggcgg ggctgaatgt gacggttaca gtacagtgat ggttgcaggg atataaggag
     7381 agaagagaat gaaaaaggtg acgttgtttc tgtttgctgc cagccacctg ccttcctgtg
     7441 tactggcctg gaacacgcca ggagaaaatt tcagcggaga gcttaagctg gaaggcccgg
     7501 tgaccagtac ccgtaacccg tgggtatgga aagtcgggca gggaaatgga ggtctggagg
     7561 ttaaacagag tggcggtggg ggggggattc aggtcgcact gccggcgatg accgttttac
     7621 tgggaaaaac caccctgacc acaccggcag gacgtgaggg gctttcaccc cgggtcagtt
     7681 acggaaaagg cactgagggg ttttcacttg tatggacagc gcctggcatg gctgaagtga
     7741 cgctacctgt caccggagat aataatgccc gggcaggcac atttgttttc agaattcagg
     7801 ctgtgggggt gttgcgccat gtgcagggcg gacaaccggt gtatgttggt atgtatgacg
     7861 acctgaatac gaatggtttg ccaggtgaaa gcactgccat gaaggtttct gatattccgg
     7921 gcgttctgca gaagatgttc agtggggaag gccccggctg gcttcagaca atgacactca
     7981 gtggttattc aggggtgagt catttcagtg atgacttcct gcgccaggtt gaaggcgtgt
     8041 atggtgcaca gactgtggct ggcagtggtg aattacggct gaacggcgag atgccggaac
     8101 actggagggt gtcactgtca gtaaacattg aatatcagta aggagcagga gtgcgtttta
     8161 taatccggag ataaggaacg tcggttactg taagattggg gagttgtgtt gatagcggga
     8221 gtggctggaa taagtggtat acatagataa aaattaaaac tatcactatt tgagtgagtt
     8281 ttttcctgct ctccatgggc tttatagctc acaaaaaata gaataatatt attaattatt
     8341 ctgctttttt atgggcattc ccccttgaaa aatgagtttg tgccagaata ttattaatat
     8401 gttttttgtg atttactaat caaggattgc tgaatagcat ccagtactga atattttgtt
     8461 caattatgtc aggaaccata ccgaaaatca aaacaggtga aaatgttctg tctgcagcca
     8521 ttcagcgcat tgaatggcta tttggaacgt ttccttctgt ctgcttgtcc ttttctggtg
     8581 gtaaggattc taccgtgctt ttccatcttg tgtccgaagt ggcccgcaaa aagaggcgtc
     8641 gtttttcggt attgttcatt gactgggaag cacaatatca atgcaccatt acgcatattc
     8701 agaagatgca gaagatgtac ctggatgtga cggacaggtt ttactgggtg gcccttcccc
     8761 tgactacggt aaacggtgtc tctcagtttc agccggagtg gatatgctgg gagcctggtg
     8821 tgacctgggt tcgccatcca ccggaatatg ctattacgga tatggcgtat tttccctttt
     8881 accgatatgc catgaccttt gaggaattta tcccggcgtt ttcctcctgg tttgccaggg
     8941 ataaatacct ggtggctgtt ctgaccggag tccgggcaga tgagtccctc aaccgcttta
     9001 tgggactggt atcccgtcgc aaacagagat atgccgatga taaaccctgg actacggcat
     9061 cacctgaagg gttttattac accatgtatc cgttgtatga ctggaaagcc cgtgatatct
     9121 ggatatataa cgccagaacc cgagctatct acaatcccct gtatgacctg atgcaccgtg
     9181 cctgtgtgcc gttacgcaac atgcgtgtct gtgagccttt tggtcctgaa cagcgtaagg
     9241 ggctgtggct ttatcatgtt ctggagccgg aaacctgggc caggatgtgt gagcgggtgt
     9301 cgggtgctgc cagcggggcg ctttatgcca atgagagcgg tgcctatttt gccctgcgta
     9361 agcgtatcac aaagccgcct catcatacct ggcgtagcta tgcgatgttc ctgctggatg
     9421 tgatgccgga aagaacggca gaacattacc gtaataaaat tgctgtctac ctgcgctggt
     9481 atcagacgcg gggcttcccg gatgacatcc cggatgaaca ggagaatgac ctggggagcc
     9541 gggatatccc gtcctggcga cgtatctgta agacactcat aaagaatgat ttctggtgcc
     9601 ggactctctc cttcagtccg aacaaacccc ggcactatga acgttacctg cagcgtatga
     9661 aggaaaggag gaaggaatgg gggattctgt gacaccggag atacttggca acatgattcg
     9721 tcagtatttc agtcaggtac gaactgaaaa agagagcata caggctctga atcatctgcg
     9781 cagagtgctt catgaggtca gtccttttgc acaggagcct gtggactgtg tgttgtgggt
     9841 gaaagcggat gaggtggtgg ccaacgatta caaccctaat gtcatggcac taggtgagaa
     9901 aaaactactg aaacagtctc tggagaaaga tggtttcaca caaccggtgg tggtgtcaga
     9961 ggagaagaat cattatctgg tggtggatgg ttttcatcgt cagttactgg ggcgggaagc
    10021 agatacagga aaacgcctga ggggctggtt gcctgtagcc tgtattaatc cggagagaaa
    10081 gggacaggcc gcacgtattg ccgcgaccat tcgacataac cgggccaggg ggaaacacca
    10141 gatcacttta atgtctgata ttgtcaggga tctttcacgt ctgggctgga cgaatgaacg
    10201 tattggtaca gagctgggaa tggaccagga tgaagtgctg aggctgaaac agattagcgg
    10261 gctgacagag ttgtttcagg aagaggattt cagctcggct tggacggttc gttgatgttg
    10321 ttaaatatgg gcggtatttc tgaaagttga tactttgggt agaaaaaatg ataccgaaaa
    10381 ctatggagga tgtgcttgtc aggtgactgg acaccatagg tcttaggctt tgataacggg
    10441 ataagatgag ctgcttttct cataccatca ggtgtgatga tggcgcagat ttacaaaatc
    10501 tgattgattc taaagatgta taagtaaaaa tcaagctttg atatgaaaaa acagatattt
    10561 attaataata aacctcctgt agtcccttac tcagggacgc acgcgaaaat atttaaatac
    10621 attgaaatcc ctctgccgtt tttctacttt atctatacat caggcgaacc attccacata
    10681 tctgtccaaa atactgttat ttatgtatca aaatacaatg gtatatttat taataaatta
    10741 gttccttttt ctcttttatt tgacagggat atatctgttc ttcaaagaag agatatttgt
    10801 gttgtgcgat ttacgtcaga ggaaataagt gaacataacg ttttatttga tcatgacatt
    10861 gaacgattaa aaaaaatttc caaagctcag cttatatctc ccgattatgt tttgattgat
    10921 ttttctagtg gggggggaga tgaaccctat gcaatgtcct ggttaatatc tcagtgtgct
    10981 catcagtgca ctgataataa aaagacggag acagatgcca tttacgataa agttcggagt
    11041 tcttatttgc tttcatgtat tctgaaaaaa aacaaaaatg ttgggcttat tcttcatgca
    11101 ccatcatttg tttctgtgtc tgaaaaaatt gccagaattg tgatggcaaa ttactcccgt
    11161 aactggagta attccgagtt ggccagtgcg gttttaatga gtgaatcttc tttaaaaaga
    11221 agaatgtaca aagaggtcgg ttctataagt acatttgtac ataaaataaa gcttacagaa
    11281 gctatcagga aattacgaag gacaaatact cccatttctg tcatatcatc tgagttaggt
    11341 tacagttcac catcgtattt ttctaaagtt ttctttaaat atttaaagac ataccctcaa
    11401 aacataagga agaaaaatgg tcggtaatgt caccaattag gaggacttgc agagccgctt
    11461 ttttacgata ctcaccccgt aagtaagcga taattggtaa cttaaacgcc catctgacat
    11521 agatggacac ctgagtatgg aattacagga ctggcgaaaa gaacctcgta aaaagtattt
    11581 ggtgcaaatc gttggttcct ttgctgaatt tgaacgcgcc atgcttcgtg aacggacaaa
    11641 gagtggactt gcagcggcac gccaggacgg gttggtggat ggcgactaaa gttaacaccc
    11701 cagcaacaaa aagagattgt ctcgctcgtc acatcggggc agaaaacagg tgctgatgcc
    11761 gcccgtctgt ttcgggttca cccttccacc gtggtgcgtc ttctcgccag acaccggatg
    11821 actgagatca aacagacata atctatgcgg agatttacag cagggcatgg ttcacaaatc
    11881 tgacagcgat gaattatcgg cactgtgggc agaaaatgct cgaatcatca agcccctgtt
    11941 acctcctgaa cccgccacac cacgggccgg ac
//
LOCUS       DADLSE010000111        11872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLSE010000111 DADLSE010000000
VERSION     DADLSE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.111"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1209
                     /locus_tag="JN692_003041"
                     /pseudo
     CDS             1..1209
                     /locus_tag="JN692_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            1432..1929
                     /gene="ubiC"
                     /locus_tag="JN692_003042"
     CDS             1432..1929
                     /gene="ubiC"
                     /locus_tag="JN692_003042"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7265293.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1942..2814
                     /gene="ubiA"
                     /locus_tag="JN692_003043"
     CDS             1942..2814
                     /gene="ubiA"
                     /locus_tag="JN692_003043"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7265294.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN692_003044"
     CDS             complement(2969..5392)
                     /gene="plsB"
                     /locus_tag="JN692_003044"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7265295.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5563..5931
                     /locus_tag="JN692_003045"
     CDS             5563..5931
                     /locus_tag="JN692_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7265296.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            6041..6649
                     /gene="lexA"
                     /locus_tag="JN692_003046"
     CDS             6041..6649
                     /gene="lexA"
                     /locus_tag="JN692_003046"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7265297.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6668..8047
                     /gene="dinF"
                     /locus_tag="JN692_003047"
     CDS             6668..8047
                     /gene="dinF"
                     /locus_tag="JN692_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7265298.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            8163..8372
                     /locus_tag="JN692_003048"
     CDS             8163..8372
                     /locus_tag="JN692_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7265299.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN692_003049"
     CDS             complement(8414..8929)
                     /gene="zur"
                     /locus_tag="JN692_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7265300.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            9247..9501
                     /locus_tag="JN692_003050"
     CDS             9247..9501
                     /locus_tag="JN692_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265301.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            9525..10232
                     /locus_tag="JN692_003051"
     CDS             9525..10232
                     /locus_tag="JN692_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7265302.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            10595..10717
                     /locus_tag="JN692_003052"
     CDS             10595..10717
                     /locus_tag="JN692_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAY7265303.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDRMYRF"
     gene            10953..11525
                     /locus_tag="JN692_003053"
     CDS             10953..11525
                     /locus_tag="JN692_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HAY7265304.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRQSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHNVSLRSDPREMDADE
                     VFDELSRADIRILDEIYSRYGHMNRFDLANMTHLESVCPEWHDPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
BASE COUNT         3042 a         2595 c         2978 g         3257 t
ORIGIN      
        1 tcttttttat catcaaatct acaacatgta actttcgaat gtaaagaaat tgagaattgc
       61 aattttacta aggcaacagt tgataatgtc atatttaaat gtcgacgttt acacaatgtg
      121 atttttctca aaacgagtgg cgaatgtgtt gattttagcc aaaatattct tgatacagtt
      181 gacttctcgc agagtcaact tggtcatagt aattttcgcg aatgtcagat tagaaattca
      241 aacttcgata attgttatct ttacgcttcg cacttcacca gagcagagtt tctgtctgcc
      301 aaagaaatat catttattaa atcgaatttg acagctgtta tgtttgatca tgtgcgaatg
      361 tcgacaggga atttttaaga ttgcattaca gaacaattgg aattaactat tgattattca
      421 gatatatttg ggaatgaaga tctcgatggt tatatcaata acattataaa aatgattgat
      481 acattgccag ataatgcaat gatattgaaa tccgttctgg cagtaaaact ggtgatgcaa
      541 ttaaaaattc tcaatattgt taataaaaac tttattgaga atatgaagaa aacatttagc
      601 cattgtcctt atataaaaga tccaattata cgtagttata tacattctgg ggaagatgac
      661 aagttcgata attttatgcg tcaacatcga ttcagtaagg tgaatttcga tacccaacag
      721 atgatccatt ttattaacag atttaatatg aataaagggc tggttgataa aaataacaat
      781 ttttttatcc aacttatcga tcaggcctta cgatcaacgg atgatatgat caaagcaaat
      841 gcctggtatc tttataaaga gtggattcgt agtgatgatg tttcacctat atttatagaa
      901 actgaagaaa agttaagaac ctttaacact aataaattaa cacgaaacga taatatattt
      961 atcctgttct cctctgtcga tgatgggcct gttatggtgg taagctccca gcgcttacat
     1021 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
     1081 cataagatgt tgcctattaa tcttactcag gaaacacttt tcagctccaa atctcatggt
     1141 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaataaa
     1201 ggtatttaag taaaggaaaa catcaccgtt cctagcatca tgggcggtga tgcccctatg
     1261 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
     1321 atacgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
     1381 aacacttttc tgcgatacaa tgcctttaag ttatgtaacg gagagttcgg catgtcacac
     1441 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggatccg
     1501 caactgctcg actggctgtt gctggaggat tccatgacaa aacgttttga acagcaggga
     1561 aaaacggtga gtgtgacgat gatccgcgag gggtttgtcg agcagaatga aatccccgaa
     1621 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
     1681 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tatcaacatt aagcgggccg
     1741 gaactagcgt tacaaaaatt gggtaaaacg ccgttaggac gctatctgtt cacatcatcg
     1801 acactaaccc gggattttat cgagataggc cgtgatgccg gactgtgggg gcgacgttcc
     1861 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
     1921 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
     1981 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     2041 tgggcgttgt gggtggcgac accgggcgtt cctcagctct ggatcctggc ggtgtttgtc
     2101 gcgggtgtct ggctgatgcg cgctgctggt tgcgtggtga atgattatgc tgatcgcaag
     2161 tttgatggtc atgttaagcg cacggcgaac cgaccacttc ccagcggcgc ggtaacagag
     2221 aaagaggcgc gcgcgctgtt tgtagtgctg gtactgattt cgtttttact ggttctgacg
     2281 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cattggcgtg ggtgtacccg
     2341 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     2401 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     2461 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcgatggt tgaccgcgat
     2521 gatgatgtga agattggcat taaatccacg gcaatcctgt tcggccaata cgataaattg
     2581 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     2641 ggcttaggct ggggatatta ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa
     2701 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     2761 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     2821 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     2881 gcctacccgg tgaatcgcaa tttattgaat ttgcaggtca tggtaggccg gataaggcgt
     2941 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     3001 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     3061 ctggttcggc atcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     3121 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     3181 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     3241 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     3301 gctgtagggt gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     3361 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     3421 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     3481 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     3541 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca
     3601 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     3661 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     3721 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     3781 gctgacgtga tgccagtagc gcagtacagc acaggttcat ggcgtttgcc gcgcctgcgt
     3841 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     3901 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     3961 aggtcatcaa cggcattggc tcaccgaagt tgacgtaacc ctgaccgaga ttacgtagct
     4021 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     4081 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     4141 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     4201 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     4261 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     4321 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     4381 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     4441 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     4501 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     4561 atcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     4621 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     4681 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     4741 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     4801 agtgcatacg cgccacgcgc gccagtttct gagcgatagt tttatccgtg ccgtgttcat
     4861 ccgccatacg gcgcagcgac actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     4921 acagtacagc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg ttcacttcgc
     4981 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     5041 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa aagcttaata gactcttctt
     5101 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     5161 gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa atcatgtgcc agacactggg
     5221 cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     5281 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     5341 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     5401 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     5461 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     5521 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     5581 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     5641 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     5701 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     5761 atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     5821 taccatgagc tttccggacg cgcaaaagat atgggatccg ccgcggtgct gattgccatt
     5881 atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     5941 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     6001 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     6061 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     6121 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     6181 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     6241 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     6301 gcttctggcg caacagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     6361 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     6421 tggcgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     6481 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggcaata aagtcgaact
     6541 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     6601 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     6661 gaccgcgatg ccgcctggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     6721 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     6781 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     6841 tagtccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     6901 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     6961 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggccgg
     7021 ggcgttaatt gcgctgttgc gtacgccgat tatcgatctg gcgctgcata ttgttggcgg
     7081 tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgctcc
     7141 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     7201 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctggt
     7261 gatggggctg catatgaacg tgcagggcgc ggcgctggcg acggttattg cggaatatgc
     7321 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     7381 cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     7441 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc
     7501 gcgactgggg agtgacatta tcgctgttaa cgcggttctg atgacgctac tcacctttac
     7561 cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg cactccggtc aggcatacgg
     7621 tgcgcgcgac ggtagccagt tgctggatgt atggcgggca gcgtgccgcc agtcggggat
     7681 tgtagcgtta ctgttttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     7741 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     7801 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     7861 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     7921 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     7981 cgggctttct ctggcggcta tctggcggcg tcactggcgc aacggtacct ggtttgccgc
     8041 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     8101 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     8161 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     8221 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     8281 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     8341 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     8401 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     8461 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     8521 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     8581 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcaaataaac atggctgacg
     8641 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     8701 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     8761 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     8821 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     8881 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     8941 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     9001 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttgtgttg ccttgtcgta
     9061 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     9121 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     9181 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     9241 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     9301 aatcaattcg aaaataaaga tgcaaccaaa gataaattgg ataatagagg ttcaatcaaa
     9361 ggtaaattaa ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat
     9421 ataattattg caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca
     9481 cgtaaaaaaa tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt
     9541 atactgacga ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg
     9601 accgggctat ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg
     9661 caatgcccat tgaacaatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg
     9721 ttcagctatt ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag
     9781 caataggttc tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct
     9841 taaaattatt tgcttcgctg ttaatttcac tttcatattc tagtgcctac gcagatttat
     9901 cagaaacagt gtatattaat gaaaataatg agagctacct gaaagctcag tttgaaaaat
     9961 tatatcaacg tgatatgaag aagtacctgg gagagatgaa gcgtctggca gatgggggag
    10021 aaatgaattt tgatggctat ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg
    10081 atcctgatat tttaagcaaa atgcgagatg ctgcaccaca attaattagc ttcgcgaagt
    10141 cgtttgaccc aacctcaaag gaagagatta aaatacttac agacacttct aaattaattt
    10201 atgacttgtt cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag
    10261 gccttatata aaaggaccca atatttattg ggttcttttt tctctatcaa tgctattagc
    10321 agggagatat atcaccagag tttaatgtgt gattttttat ttatcgtcga acatggattg
    10381 tttatcattg gccttaacaa agttaacggc taataagatt atttccatca cttcgtgaga
    10441 gcttcatgcc ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt
    10501 tatggtatag taccccgcta ttgagcctcc tgaacagtga agctgaataa cataacccca
    10561 tgatatatcg ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa
    10621 aatcaaccaa accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag
    10681 cgttgcacca atgctcgata ggatgtaccg tttttgaaaa acaactagtt atacactttg
    10741 tgggagccta ttgggaacct gttattctta gaaagcatca tttgctcatc acttcaattg
    10801 accatggtta aagccagcta taaacttgac aaacgaggta actggttgct taggcccaag
    10861 tgggcacacc ataagaagct catgttggtg atgaagcaac ccatgaagta tcgagaataa
    10921 ttataaagtt cactatcttt gaggtttctt atatgttttg cgaagaaaaa gtagctcaaa
    10981 tggctgcata cctacttctt aagagaggtg ggcgcatggc atatctgaaa ttgatgaagc
    11041 tgctctatct gtctaaccgc cagtcgattt tgaagcatgg caggatgatc ggcgaagata
    11101 gcctttactc tatgaaattt ggaccagtca tgtcgaatac gctgaacttg attcgcggta
    11161 aggctgaagg cattggtgac tactggtaca acttgataga gacgaacggg cataatgtat
    11221 cgttgcgttc agatccgagg gaaatggatg cagacgaggt ctttgatgaa ttgagtcgtg
    11281 cagatatccg gattttagat gaaatctatt ctcggtatgg gcatatgaac cgatttgatc
    11341 tcgcaaatat gacgcattta gaaagcgttt gtccagagtg gcacgatcct ggcaattctc
    11401 gtaagcctat agacctgaaa gaaatgctga tcagtgaggg taaaagcgag gatgaggcta
    11461 atcgcataat tggcaaaatg gaagaatctc agaaacttaa ggaattttct ttgcaattat
    11521 catgacggat tatcagccat acaggaaagg aactgtgctt gccccaactg gaccatgcaa
    11581 tcatcttcat gtgatttgta atgatcctgt ttattacccc gttaacgatt gttattgtgt
    11641 tttagttgtt aatatttcta gtatcaagga tggtgtcccc cacgatccgt cttgcgtctt
    11701 gaattctggt gatcatcgct ttatcaagca tccaagttat gttgtttacg ctgaagctat
    11761 aatttggcga gtggataaca tggttagaaa gcagcgatcg ggtgagattt ctgttcatga
    11821 tgatatgcca gaagctacat tcaatagaat tctggacggt tttgatatct ct
//
LOCUS       DADLSE010000112        11862 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLSE010000112 DADLSE010000000
VERSION     DADLSE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.112"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            252..699
                     /locus_tag="JN692_003054"
                     /pseudo
     CDS             252..699
                     /locus_tag="JN692_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134906.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            699..1691
                     /gene="lsrD"
                     /locus_tag="JN692_003055"
     CDS             699..1691
                     /gene="lsrD"
                     /locus_tag="JN692_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HAY7265305.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTATAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            1703..2725
                     /gene="lsrB"
                     /locus_tag="JN692_003056"
     CDS             1703..2725
                     /gene="lsrB"
                     /locus_tag="JN692_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HAY7265306.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYGERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            2752..3627
                     /gene="lsrF"
                     /locus_tag="JN692_003057"
     CDS             2752..3627
                     /gene="lsrF"
                     /locus_tag="JN692_003057"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7265307.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3651..3941
                     /gene="lsrG"
                     /locus_tag="JN692_003058"
     CDS             3651..3941
                     /gene="lsrG"
                     /locus_tag="JN692_003058"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7265308.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3980..4738
                     /gene="tam"
                     /locus_tag="JN692_003059"
     CDS             3980..4738
                     /gene="tam"
                     /locus_tag="JN692_003059"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7265309.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4742..5656)
                     /locus_tag="JN692_003060"
     CDS             complement(4742..5656)
                     /locus_tag="JN692_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HAY7265310.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(5863..7314)
                     /locus_tag="JN692_003061"
     CDS             complement(5863..7314)
                     /locus_tag="JN692_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7265311.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLVGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(7541..8959)
                     /locus_tag="JN692_003062"
     CDS             complement(7541..8959)
                     /locus_tag="JN692_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7265312.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(9098..9457)
                     /locus_tag="JN692_003063"
     CDS             complement(9098..9457)
                     /locus_tag="JN692_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7265313.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN692_003064"
     CDS             complement(9457..10383)
                     /gene="glsB"
                     /locus_tag="JN692_003064"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7265314.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(10447..11406)
                     /locus_tag="JN692_003065"
     CDS             complement(10447..11406)
                     /locus_tag="JN692_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7265315.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIGAQAGAALKKCVLELGGSDPFIVLN
                     DADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDE
                     ENALGPMARFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTA
                     FREEMFGPVAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFI
                     NGYCASDARVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            11486..>11862
                     /gene="yneK"
                     /locus_tag="JN692_003066"
     CDS             11486..>11862
                     /gene="yneK"
                     /locus_tag="JN692_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7265316.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEIN"
BASE COUNT         3076 a         2973 c         2937 g         2876 t
ORIGIN      
        1 gacagacact gcgctcttag catggggcag ggaataacca ggctatttct ccatgcacag
       61 gccaattatg aaagaggaga ggcttaagtt cttgaaccct gaacgaataa cgactaccag
      121 aaaagcactt cgcatcactc agacatgatt taacataata tacattatgc gcagtaacat
      181 tgtagtagct aaattgagat gtccgtctct gactaaatag acatgtccgc gctacagtct
      241 caccacagcg attgctgctt ggcatttcag caattggttg gttgacgata attctggtgg
      301 catttatggc ctggctgcat ggcggcactg gcgggaattg tgtttgcttc gcagattggt
      361 tttatcccca accagaccgg taccgggctg gagatgaaag caattgcagc ctgcgtgctg
      421 ggcggcatta gtttgctcgg tggttccggt gcgatcatcg gtgcggtact cggcgcatgg
      481 ttcctgacgc agatcgatag cgtactggtg ctgttgcgca ttccggcatg gtggaatgat
      541 tttatcgcgg gtctggttct gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg
      601 ctggaacgta atctacggcg gcaaaaatat gcccgcttta tgacgccacc gccatccgtt
      661 aaacccgctt cgtcaggtaa aaaacgggag gccgcataat gcgtattcgc tacggttggg
      721 aactggctct tgccgcactg ctcgttattg agattgtcgc atttggtgca attaacccgc
      781 gaatgttaga tctcaatatg ttgctgttca gcaccagtga ctttatctgc attggcattg
      841 tcgccctacc gctgacgatg gtgattgtca gtggcgggat cgatatttcg tttggttcga
      901 ccatcggcct ctgcgccatt gcattgggcg tactgtttca aagtggtgtg ccgatgccgc
      961 tggcgatact cctgacctta ctgctcggcg cattgtgcgg gctgatcaac gccggattaa
     1021 ttatctatac caaagttaac ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg
     1081 gaagcgctct gctgctttcc ggtatggccg gagcgacggg gtacgaaggt attggtggat
     1141 tcccgatggc gtttacagat ttcgctaacc tggatgtgct gggactcccc gttccgctga
     1201 ttatcttcct gatatgtctc ctcgttttct ggctctggct gcataaaacc catgccggac
     1261 gtaatgtgtt tttgattggg caaagcccgc gcgtggcgct ttatagcgcg attccagtta
     1321 accgcacctt atgtgcgctc tatgccatga cggggctggc gtctgcggtc gccgctgtgc
     1381 tgctggtatc gtattttggt tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg
     1441 ccatcaccgc agtggtgctt ggcggggcca atatttatgg tggttccggt tccattatcg
     1501 gcaccgccac tgcggtttta ttagtgggat atttgcagca aggtttgcaa atggcaggag
     1561 tgccaaatca ggtgtccagc gccctttccg gtgcgctgct tatcgtcgtt gtcgtaggtc
     1621 gttccgttag cctgcatcgc caacaaatta aagagtggct ggcgcgtcgg gccaataacc
     1681 cattgccata aaggatatct tcatgacact tcatcgcttt aagaaaatcg ccttactgag
     1741 cgctcttggc attgccgcaa tctctatgaa tgtgcaggcc gcagagcgta ttgcatttat
     1801 tcccaaactg gttggcgtgg gattttttac cagcggtggc aacggcgcac aacaagcggg
     1861 taaagagctg agcgttgatg tgacctacga cgggccgaca gaacccagtg tttctggtca
     1921 ggtacagttg attaataact tcgtcaatca aggttataac gccattatcg tttctgcggt
     1981 ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg caacgtggtg tgagagtgct
     2041 gacctgggac tctgatacta aaccggagtg ccgctcttac tacattaatc agggaacgcc
     2101 cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt caggtgaata aagacaaagc
     2161 caaagtcgcg tttttctact caagccccac cgttacggac caaaaccagt gggtgaaaga
     2221 agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa attgtcacta cgcagtttgg
     2281 ctataacgat gccactaaat cgttacaaac cgcagaagga atattaaaag cgtatagcga
     2341 tctcgacgcc attatcgccc ccgatgccaa cgccctgccc gctgccgcac aagccgcaga
     2401 aaacctgaaa aatgacaaag tagcgattgt cggattcagt acgccaaatg tgatgcgccc
     2461 gtatggagag cgcggcacgg tgaaagaatt tggactgtgg gatgtggttc agcaaggcaa
     2521 aatttcagtg tatgtcgcgg atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa
     2581 gctggatatc cagggcgtag gtcaggttga agtctcgcca aacagcgttc agggctatga
     2641 ctacgaagcg gatggtaatg gcatcgtact gttaccggag cgcgtgatat tcaacaaaga
     2701 gaatatcggc aaatacgatt tctgatgtgc ataacttaac cggagtaagt tatggcagat
     2761 ttagacgata ttaaagatgg taaagatttt cgtaccgatc aaccgcaaca aaatatccct
     2821 tttaccctga aaggttgcgg tgcgctggat tggggaatgc agtcacgctt atcgtggata
     2881 tttaatccga aaacgggtaa aactgtgatg ctggcctttg accatggtta ttttcagggg
     2941 ccgactaccg gacttgaacg cattgatata aatatcgccc cgctgtttga acatgccgat
     3001 gtattaatgt gtacgcgcgg cattttgcgc agcgtagttc ccccggcgac caataagccg
     3061 gtggtactgc gggcgtcagg tgcgaactct attctggcgg aattaagtaa tgaagccgtg
     3121 gcgttatcga tggatgatgc cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat
     3181 atcggcagcg aatatgaaca tcagtcgatc aaaaatatta ttcagctggt cgatgccgga
     3241 atgaaagtgg gaatgccgac catggccgtg actggcgtgg gcaaagatat ggtgcgcgat
     3301 cagcgttatt tctcgctcgc gactcgaatc gccgctgaaa tgggggcgca aattatcaaa
     3361 acctattatg tcgaaaaagg ttttgaacgg attgttgccg gatgtccggt acccattgtt
     3421 attgctggcg gtaaaaaatt accggagcgc gaggcgctgg aaatgtgctg gcaggctatc
     3481 gatcagggcg cttctggtgt ggatatgggg cgtaatattt tccagtctga ccatccggtg
     3541 gcgatgatga aagccgtaca ggcggtggtt caccataacg aaacggctga tcgggcatat
     3601 gaactctatc tgagtgaaaa acagtaactg cggatttaag gagaagaatt atgcacgtca
     3661 cactggttga aattaacgtt catgaagaca aggttgacga gtttatcgaa gtttttcgcc
     3721 agaaccacct gggctctgta caggaagaag gtaatttgcg cttcgatgtc ttacaggacc
     3781 cggaagtgaa ttcgcgcttt tatatttacg aagcctataa agatgaagac acagtggcat
     3841 tccataaaac cacgcctcac tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg
     3901 ggccgcgtaa aaaacgtctg ttcaatgggt tgatgccgtg aggctacaat tggggtaacg
     3961 cgctgacggg agtaaaaaaa tgtctgactg gaacccctct ttatatctgc acttttccgc
     4021 tgaacgatcg cgtccggcgg tggagctgct tgccagagtg ccgctggaga atgtcgaata
     4081 tgttgccgat cttggctgtg gtccaggtaa cagcaccgcc ctgctgcatc aacgttggcc
     4141 tgcggccagg ataacaggca tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc
     4201 tttgccagac tgccagttcg tggaagcaga catccgcaac tggcaaccgg aacaggcact
     4261 cgatctgatt tttgctaatg cctcactgca atggctgccc gaccactacg aattgtttcc
     4321 tcacttggtt tctttactta atccgcaggg cgtgctggca gtacagatgc cagataactg
     4381 gctggagccg acccatgtgc tcatgcgcga agttgcctgg gaacaaaact acccggatcg
     4441 cgggcgtgag ccgttggctg gcgttcatgc ttactacgat attttgagcg aagccggatg
     4501 tgaggtcgat atctggcgaa ccacttacta tcaccagatg ccgtcgcacc aggcgattat
     4561 cgattgggtg accgccactg gattacgtcc gtggttacag gatctgaccg agagcgaaca
     4621 gcagcttttt cttacgcgct accatcagat gctggaagag cagtatccac tgcaagagaa
     4681 cggacagata ctgctggcat ttccgcgtct gtttattgtt gcccgccgta cggagtaatt
     4741 atcacgtcag ctggtaatga cgatcgggaa gaatttttgc tggaatttct gcttcatcgt
     4801 tcatctgtaa caggtcaatt tcaatagcgt tgcagatggc atccagcggt aaatcattgt
     4861 tttcagtacc gaacggatct tccagttctt ccgccagaca atccagcgaa ataaaagtgt
     4921 aggaaatcag cacagagata aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg
     4981 gcagcatgat acaaaacaga taaacggtac gatgcaaaat cagggtgtag gcaaagggaa
     5041 ttggcgtgta ggcaatgcgc tcacagcctg ccagaaccgc tgaaatatca ttaagccgat
     5101 cgttgaggct aataaacagg atatctgaaa gctgtccatt gcggcgctga accgccaacc
     5161 attctcccat tattaacaag atacggttag ctggagagtt cgaagccagt acacgctgaa
     5221 gatcttcagt cttgagataa tgagccagca cttccgcctg tggctgtttg cgtaatgtca
     5281 tgcgtaaaca gtgggcgaag gcgatttgca gcctggcaaa ctcccttaca cttgccgaat
     5341 ccggcaatgt cgtttttacc tctcgcagta acgaccgtga ggcaatcatc aactgccccc
     5401 aaagttttcg cgcttcaacg taacgggcgt acccggcatt attacgaaaa ccaagaaaaa
     5461 tggcgatggc gacaccgaga atgctgaacg gtgcgagggt gaatttgatg cccagatgcg
     5521 tgtaccaggg cagcatgaat ataacagcaa tagaaaagag aaaattgagt agtaagcgcg
     5581 aggatatctt ggataatact gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt
     5641 gtggacgaac aatcatggtt atcttcaggc gtggaaaagt cgccctatta aacgtgatta
     5701 cgatcacatt ctcaagacgc tacttacaaa ttacctacaa gcttacaaca ttaacaggcg
     5761 ctgcatgtga cacctgttaa tgataaaggc atataggatg ttgtaactaa tatagtgaaa
     5821 taagaaaccc ggtcgaaacc gggttcagaa gtaacggtgt tattagcaca acggacgtac
     5881 agcttcgcgc atcccttttt caagaatcgc atccaggtca ttagcaacct gctcgaccag
     5941 accaggcact tgcgtcaggt cctgctccca gtggtctttt tctgccaaca caatcgctac
     6001 cagttcctgg gtaccgataa cacggtcacg atgctggctc cagagttgct gataacgttc
     6061 cagccagtgt gcatcgtcct gtaccggata tgtttctccg ttacgctcac cacgatagaa
     6121 cgcaatcaat gccgctaatg cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg
     6181 ccctaccagc agctgcggca ggatgcgggt gcggaacttg gtcataccgt tgagcgcgat
     6241 agacagcagc tgatgcttaa tgtacgggtt acggaaacgc ccggtgactg cactggcgaa
     6301 agattccagt tcatcacgag gcaaatccag taccggaata atttcttcgt agatggcttt
     6361 ttcaacgaat gcgcaaattt cagcatcgtt catcgcttca cctacggtat ccagccctgc
     6421 ctggaacgcc accggcacca gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc
     6481 tttatacggt ttaatatcgt caacaatcag cacgttgagc ggatatttgt ccaggcgcag
     6541 ctcagtcgct aaggatttcg gcccctgaat cacaaacagg taaaagtgtt cagcggtgtc
     6601 gaggaaacca tcgtgataac ccagttcttc ttccagttta gccacttcat cgcgcggata
     6661 accggtaacg atacggtcca ccagcgtaga acagaagctg ttagcctgat ccagccattg
     6721 aataaatgct tctggcagtg cccactcttg cgcatagcgc agcaccagtt cacgcaacgc
     6781 gtcgccattg tagtcaatca actcacacgg aatgatgatc caacctttat ccagcgcacc
     6841 gttgaaatgg ctaaagcgtt cgaacagcag acgggtcagt tttgccggat agcttaccgc
     6901 tggcgcgtca tcgaatttat cgcccgcatg gtagctgata cccgcttcgg tggtgttaga
     6961 gaaaacaaag cgcatttccg ggttgtgcgc cagtttcagg aattcatcgt attcactgta
     7021 gacgctgatt tcacgattaa ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt
     7081 ctcattcagg ccacggataa tggtggtgta cagaccatcc tgcgtgctca gtgacggcgg
     7141 gaatgaagtt tcaatcggac gaacaacgac cacgccagaa ttcagatcgg tgtgctcatt
     7201 caggagatcg atttgccagt caacaaaggc gcgcaggaag ttaccttcac caaactgaat
     7261 gatacgttct ggatactgtg caccgggaaa atcgcgacga tttagtgttt tcacaatggg
     7321 ttcccttctg attagtcata caacctgttt gaattggtgc gacaggttag caaactttaa
     7381 tacgccgaac ccctgttttg atcaactcct gatgattaat gagcagtttt atgagaaaag
     7441 tgtggcgcga atcatggttt aatcgaggaa atacgccttt tcctggatca taaagtggta
     7501 gaacacattg cattcaaatc gcgcggaatg aataaagatg tcagacaact tcctcaccgt
     7561 aacgcatagt gctggtacgg ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg
     7621 cttcgaccat caatttatta aaatcatcgg tcagcgttcg gtacgatgcg cgaccactac
     7681 cgacgccaat acttaccgtg agataaagcg ttttttgttg ccaggtgaat ggttgcagtt
     7741 caacgccttt acggattttt tccgccatta gcagaccatc gacaggattc accgacggca
     7801 ctgcaacagc aaattcttcg ccgcccatgc gcgccaccag ccccttatca ccgacaatct
     7861 tctgaatatg ccgggcaaac acgcttaaca ctttatcgcc acattcatgt ccatagttat
     7921 cgttaatgct tttgaaatag tcgatatcaa gcagcatgac cgtcagatgt tgtgtctgtt
     7981 tcagcgatgg acttttcagc gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa
     8041 aatcaaagtc ggctcgcagc gcaacttgct tcattagcaa attgatcgct gccacgctaa
     8101 aagaaaccat aattgggcat atcgccatcg tggcaatacc gagacgtgcg gagaacattt
     8161 ccggaatgga gaacggcgaa ccgaccgaga tatcaatcac cgaatttgcc accagtacga
     8221 tttccaccgc accggtgaca aaggtcaaca gacatgttac ctgcggcgta tagcgcactg
     8281 cacaccagat taatgcaggc agcgggaacg ccagactccc cgctccgcca atgactaccg
     8341 aggcaataac cgacacaata agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg
     8401 gcagcactcc aggaatagcc aacgtcagca tacaaggcac gatcaacacg cccgttgaga
     8461 attgctcact gaaccagtcc gcaagcaaag gccagaaatc cagactatca atactgaccg
     8521 aaccaatcgc cccgacaata gcgcataata atgcggcaat cagacagtaa ttaaatagcc
     8581 gtaaggcact tactggctca tacttatttt tccccagacg cttatcccgc acgaccagta
     8641 aggcgacggt aacaataaac atcatattgg agaaattgat aaccagtgaa accagtcccc
     8701 attcggtggt tatggcatca taaaccagca tcgccacata actgatcgca tagtaatgca
     8761 gtcgattaag ccagacatag cgggcaaaca cccccgccat tacgccattc agcggccaga
     8821 aaagagaaag ttcgtgaacc agccgtaact ctgcaccaat aaaatagaac agcgtggtta
     8881 gcgcaaaaat agcgatgcta ttacgtagca gatgaccttc ctggaacaaa cggaaagttg
     8941 aaatggggtg tacatgcatt aattatctgt ccatacaatt gcgggtggca attatctcaa
     9001 cgtaataagt aaattctcat aaaggatagc atgttcgcta acagcggcgg caaataaagg
     9061 taccctggga atgctatttt atcgttttcc gcaattctca tggctgattc atctgtacaa
     9121 ccagccagtg atagattacc gccacgatat aacgttgttg ttcttcgctt aacgaaacac
     9181 cctgcggaat gttatgccac ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct
     9241 gagcgataaa caccggatgc ccgcgcatgg gggtttgctt accgtcatta gccggtaatg
     9301 ccggagcaag acgtttagcg acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt
     9361 tctccaggca atactgacgc tctttcatgc ccagacgaaa gcgagagcga aattttgaac
     9421 gggataaacg cgcgaagagt ggatcgagag actgcattaa taaaccgaac gccctaactg
     9481 tttcgtcaat tgttcaagga cggcgatacc cgcaagcgag ttacctgcat catccagttc
     9541 cggactccag actgcgatgg ccatttcatg cggaacaatc gccacaatac cgccaccaac
     9601 gccagatttc gccggtagtc ccacccgcca ggcaaactcc cccgcgttct ggtacatacc
     9661 actggtcgcc atcagcgcgt taatttgccg cgcctgcatt ggcgtcacca caggttcatc
     9721 aatatgaata gctttcccct gattagccag aaagacaaac gtccgggcca gctctacaca
     9781 gctcattttc agagcgcagt aatgaaagta gttttgcaga acggttgtca cgtcatgatg
     9841 gaaattgcca aacgacttca tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa
     9901 ttcggaacgc gctaccaccg tatcgtatga aatatcagac acaccgctta agccgcgcac
     9961 gacttccagc atacgttgcc gtggtgcgct tagtcgccct tgcaacatat cgcagaccac
    10021 cagcgcaccg gcattaatga acggattacg cggaataccc tgttccattt ccagttgcac
    10081 taaagaattg aacggtgatc cagacggatc tttgccgacg cgttgccaga tttcctcttc
    10141 ggagtaatga cgcatggcga caacgagact cagcactttg gaaatagact gaatggaaaa
    10201 acgttcttgc gcgtctccgg cctgaaaaag ctgtccgtca acggtacaga tagcaatccc
    10261 caatcgggaa ccgtctactg tagccagcgc cggaatataa tccgcgactt taccctgacc
    10321 aatgagcggc cgcacttgcc gcaagatgtt ttctaaaatt gcattatcca tggcgactgc
    10381 cactttctac tcctggaccg caggtctgaa aagacctgcg agtatatcag agctgaatat
    10441 gtcgcgtcag atccggtctt tccacactgt ctggatatta cagaattcgt gtaagccgaa
    10501 atgggaaagc tcacgaccaa agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc
    10561 gctggcacaa taaccattga taaacacccc accgcattcc agacgtgccg ccatctgtct
    10621 ggcctgtgtt tcgtcggtgg taaaaatggt cgctgaaagg ccgaactcac tatcattagc
    10681 cagttccagc gcatgttctg catctttcgc aatggtgatt gcagcaacgg ggccaaacat
    10741 ttcttcccga aacgcggtca tttctggggt aacattcgcc agaaccgttg gcggatagta
    10801 gttacctgcc ccagccatct tttccccgcc cagtaacaaa cgcgcaccct gagccagggt
    10861 tttctccacc tgatgatgca gctcatcacg taaatcaaaa cgagccattg gtccgagagc
    10921 gttctcttca tcacggggat cgcccatttt caaggctgcc gcagctgaca caaaacgttc
    10981 ggtaaatgcc gaagcaattc cctcttcgat aataaagcgt tttgccgctg cacaaacctg
    11041 tccggtattc tgataacgtc cggctaccgc cgctttaacc gccagttcca gatcggcatc
    11101 gttaagcaca ataaatggat ccgaaccgcc cagttccagt acgcattttt tcagtgccgc
    11161 tccagcctgt gcgccaatag ccgctccagc acgaacactt ccggtcaccg tgacagcagc
    11221 aatgcgcgag tctttaatca tctgactgac accgtcgttg tcggcattca gccagccata
    11281 tacgccttgc gggattcccg catctttaaa cacttgggca atgagctgag cacagcccat
    11341 cacattcggc gcatgtttaa gtaagtagcc gttacctgca agaatgatgg gaacagcgcc
    11401 acgcatcacc tgcctttgat agttcttgca gctcaaatgc cctatatagt gatgttccga
    11461 tgacttatga ctatatgggg caaatatggt tacgccagta agcatcagca attacatatc
    11521 tcttcctgat gattttcccg ctcgtaatat tgcacctcaa gtaaaagaag ttttaaagga
    11581 ttttattgat gcactcagta ccataatatg tgatgaagag tggcgcacgt ctttaaacac
    11641 caactcagcc acaaaaaaga tatttaacaa ccttgataac ttatcatata ttcagaggac
    11701 ttcttttcgg ggtaacgaca cactatacaa tgaaaaggtt cagtttaaac tcacttatcc
    11761 cgtcaagaat ggaagacaca aagaaaatat tgaatttcag gtagtaataa atttaagccc
    11821 tatttattta gataattttc gccatgatgg agaaattaat at
//
LOCUS       DADLSE010000113        11756 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLSE010000113 DADLSE010000000
VERSION     DADLSE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.113"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN692_003067"
     CDS             complement(137..1501)
                     /gene="tdcG"
                     /locus_tag="JN692_003067"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7265317.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRNKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1573..1962)
                     /locus_tag="JN692_003068"
     CDS             complement(1573..1962)
                     /locus_tag="JN692_003068"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7265318.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN692_003069"
     CDS             complement(1976..4270)
                     /gene="tdcE"
                     /locus_tag="JN692_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HAY7265319.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN692_003070"
     CDS             complement(4304..5512)
                     /gene="tdcD"
                     /locus_tag="JN692_003070"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7265320.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN692_003071"
     CDS             complement(5538..6869)
                     /gene="tdcC"
                     /locus_tag="JN692_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7265321.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN692_003072"
     CDS             complement(6891..7880)
                     /gene="tdcB"
                     /locus_tag="JN692_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7265322.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSLLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN692_003073"
     CDS             complement(7979..8917)
                     /gene="tdcA"
                     /locus_tag="JN692_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7265323.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9107..9451
                     /gene="tdcR"
                     /locus_tag="JN692_003074"
     CDS             9107..9451
                     /gene="tdcR"
                     /locus_tag="JN692_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7265324.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9707..10247
                     /locus_tag="JN692_003075"
                     /pseudo
     CDS             9707..10247
                     /locus_tag="JN692_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10269..11435
                     /locus_tag="JN692_003076"
     CDS             10269..11435
                     /locus_tag="JN692_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HAY7265325.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIKRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELTKE
                     IYKSSSKINNCLNRIKLPEMKEFSSKKIYDYIDTIIEDYENIKENAYLVVPQINYTMD
                     LNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         3335 a         2839 c         2549 g         3033 t
ORIGIN      
        1 aagactttaa aataacggag atggataaga attttctact aaattaatcg caagagaata
       61 ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca cccaaggatg
      121 aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg cgtgatgttt
      181 cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc actttatcga
      241 gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt actgctttca
      301 cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca accggatcgc
      361 aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc tgcgccggac
      421 tgccgcccag tagttcagtt aaccctgccg ccgccattga acaggccacg ccaatctccc
      481 cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc gcgccaatag
      541 cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga cggaacttat
      601 cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt gccgttacca
      661 cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg atccagtcga
      721 tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga cgcagcgcta
      781 cggcacgacg cggcacattg agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt
      841 caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt tccgctttgt
      901 tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg ttgtaatcac
      961 acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt
     1021 gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag taataggttt
     1081 tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc tcatggcgag
     1141 gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca atatgcgcac
     1201 cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca gggatctcat
     1261 caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg acatccgtgg
     1321 catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata tggctcgtcg
     1381 cggttaataa gccgctactt tccagccgat caataaaact ttttccggca ttcattggcc
     1441 ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat gcactaatca
     1501 tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg cgaccgtccc
     1561 gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct tcacatcttt
     1621 cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa
     1681 gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga tgaacacggt
     1741 catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt tgacgttttc
     1801 gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct gcgggcaaac
     1861 cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa cataagggcc
     1921 gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc cggcgtcaga
     1981 gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc agtgcgttga
     2041 agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc gggtgctcga
     2101 tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga ccgccttcga
     2161 catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt ttacgtactg
     2221 gatcttcttt gcccagcgcc gcaggaacga ttgagaaagt gtacgagatc ccatctttgg
     2281 cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct ttgcggtcac
     2341 gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg
     2401 tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc gactgggtag
     2461 ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt tcaaccaggt
     2521 cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga
     2581 tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg gcatatttga
     2641 tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat
     2701 agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt
     2761 agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg aagtgatcga
     2821 ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct gttttcggcc
     2881 cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc agcgttttcg
     2941 ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt gggctgacgc
     3001 agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg tcattttcat
     3061 actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag gcaatcggta
     3121 attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata gtgtgcaggg
     3181 tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc cccatcccgc
     3241 cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat tccggtgtac
     3301 gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc tgtgcctgct
     3361 gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg aacgatgccg
     3421 tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga taagcgaagt
     3481 agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca aagccatatt
     3541 tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc tcctcacgca
     3601 gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga tcggcaaatt
     3661 gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg cgatagtcac
     3721 cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca gatttacggc
     3781 agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt ttacgcagat
     3841 cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg aatgaacttt
     3901 taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca tccgtttgca
     3961 ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg gtaattgtgg
     4021 tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata cggatgcctt
     4081 ccattacttt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga aaagattcat
     4141 cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt tcgcttttcc
     4201 agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg gtatcaatat
     4261 ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca aattctgcgg
     4321 gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct tcgttagtcg
     4381 gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg ccaaaagagt
     4441 tagatcgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa tgttccatga
     4501 ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata attccatcca
     4561 gccgacgtaa tgaagctgcg tgtccggcaa tatgacgggc aattcggtga acaaaggttt
     4621 taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga acacgtaaat
     4681 ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg cgttccaggt
     4741 cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag tcgacatcgc
     4801 cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag gtgtcaacac
     4861 tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc acaaccaggc
     4921 cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca tagcggtgcg
     4981 acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc cacggcaggc
     5041 cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat accgccacct
     5101 gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt gcgtaattat
     5161 gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg gtaataatgg
     5221 cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag gccacgctgt
     5281 catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct tcgtagctgt
     5341 ggagagccag cggtgctggc tctcccccat ttacggataa gaatgcattt tccgagttaa
     5401 taccgtcggc aatacctgac attaatactt cacagtcgct ggcatcgagt acggaaaact
     5461 taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc atctcttttc
     5521 tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt cagcagacca
     5581 atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga cggcgcttta
     5641 cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc gcccatggct
     5701 tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga gcccatgatg
     5761 aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc acctttataa
     5821 ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg accgaagaaa
     5881 gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt aatcgcgaac
     5941 gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag cactggaata
     6001 ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca gctaaaggca
     6061 aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg ggaacattta
     6121 cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga aaccacaaag
     6181 gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc cagccagaca
     6241 gtaatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac ctggtcgata
     6301 actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct ggcgataaac
     6361 ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca gatgacgaaa
     6421 gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc aaatccgagc
     6481 tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat ccacagcagt
     6541 gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt accaaaatgc
     6601 tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca cagacgcgcc
     6661 agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag aagaatcggg
     6721 atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc gattgccgtg
     6781 ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg ttttgtctgg
     6841 ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa ttaagcgtca
     6901 acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga aataatactg
     6961 acggtttttc tgttttgaat atattggtct aatttaccgc ttaataatgc agcacatgcc
     7021 agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat catactgttt
     7081 ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac gatttcgtaa
     7141 gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt agttcggtgc
     7201 gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc tgactgtacg
     7261 ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc agcaattaaa
     7321 ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc cataatttcc
     7381 agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg gataaaaata
     7441 cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa gttatcacca
     7501 tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt tggcgcacct
     7561 tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga aaccccttgc
     7621 gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc gatcagtaaa
     7681 cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat attttcaaac
     7741 ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg catgcctgtt
     7801 ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat agcaaccggc
     7861 agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg taggtaaccg
     7921 taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa acaaataact
     7981 aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac tctttggcta
     8041 attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat accgcggcat
     8101 attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta gaaccaaaag
     8161 gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca agattataaa
     8221 ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt ctttgtaacg
     8281 tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac tgttcgttct
     8341 tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct accagcacaa
     8401 actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct gcacttaacg
     8461 taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg
     8521 cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg atcatccctg
     8581 acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc accgcctcag
     8641 aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg gattcggaac
     8701 gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc cgcaccacta
     8761 attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc ggttgagtta
     8821 accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc tgaaagacta
     8881 ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt aattacctca
     8941 ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt
     9001 aactttcatt actttccagg gtatcgttat tacaattaaa cacctttaaa tatcaacaag
     9061 ttaaagcata aaaatcagca taaagcctga tttttatcaa aaaaaaatga cggggataac
     9121 catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg ggctgagacc
     9181 atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt taatgagcaa
     9241 gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg cacgtcacat
     9301 tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta aaatttcgaa
     9361 tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt ctgacaggac
     9421 gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg cgacgaatag
     9481 cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa ggtcagacga
     9541 agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga atgttgcttc
     9601 gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt cgtgcgagga
     9661 aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga aaggcttccc
     9721 aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta acaatcacaa
     9781 tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct ccaccaatga
     9841 cgttaataaa atttacagta taagtaatga actgcggaga gtattatctg caattactgc
     9901 attgaatttc tatcaaggcg atgttccttc tgtcatgatc cgaatccaac cggaaaatat
     9961 gagtccattc atcatagata tttctacagg agaacatgat gattatatca tacaaacatt
    10021 agatgtaggc acttttgcac cttttggtga acaatgtact tgctcagccg tcaataaaaa
    10081 agagctggaa tgtattaagg agacgatttc taagtattgc gcaaagttca cccaaaaaga
    10141 agccatctta acacccccta ggcactttaa taaaacctgc attacttcag attgttggca
    10201 aattctcttt tttttcaccc gatcatttta ataatgattt ttattgatta tttataaagg
    10261 ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat ttagtccgtc
    10321 gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca ctagcaccag
    10381 gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac gcgttacaac
    10441 caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac ttacaaaatg
    10501 taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac gcccaaatta
    10561 ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa aaccaggaaa
    10621 tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat gaaatcaaga
    10681 ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc tctctggact
    10741 tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct aatactattc
    10801 taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt caggttaatt
    10861 ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa ataacgcctg
    10921 ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc gaaagttgtt
    10981 taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt gcaattataa
    11041 aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca aaagaaatat
    11101 ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca gagatgaaag
    11161 aattctcatc aaaaaaaata tatgattaca tcgatacaat cattgaagat tatgaaaata
    11221 tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat ttaaacatag
    11281 aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc cggacaatgg
    11341 ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc aatattttac
    11401 cagagaggac tatacgtata attacgacct tttaaatgca atatagactt aataacttct
    11461 tacaaagtct atcattaata ccgtagatat tcatcttacc ctgtgcacca aaagcaaaaa
    11521 cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc gctacgctta
    11581 tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg caggataagg
    11641 cgttcatgca gcatccggca tgaataaagc gcactcaaca atctgaaacc cgccggaacg
    11701 gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg gtatag
//
LOCUS       DADLSE010000114        11748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLSE010000114 DADLSE010000000
VERSION     DADLSE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.114"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN692_003077"
     CDS             complement(<1..1362)
                     /gene="cusC"
                     /locus_tag="JN692_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7265326.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYLNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEVERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISNASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTAL"
     gene            1519..2202
                     /gene="cusR"
                     /locus_tag="JN692_003078"
     CDS             1519..2202
                     /gene="cusR"
                     /locus_tag="JN692_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7265327.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2192..3634
                     /gene="cusS"
                     /locus_tag="JN692_003079"
     CDS             2192..3634
                     /gene="cusS"
                     /locus_tag="JN692_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7265328.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GALDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKCEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            3784..6021
                     /gene="nrfB"
                     /locus_tag="JN692_003080"
                     /pseudo
     CDS             3784..6021
                     /gene="nrfB"
                     /locus_tag="JN692_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
     gene            6008..8980
                     /gene="nfrA"
                     /locus_tag="JN692_003081"
                     /pseudo
     CDS             6008..8980
                     /gene="nfrA"
                     /locus_tag="JN692_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            8981..9871
                     /locus_tag="JN692_003082"
     CDS             8981..9871
                     /locus_tag="JN692_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7265329.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            10054..10815
                     /locus_tag="JN692_003083"
     CDS             10054..10815
                     /locus_tag="JN692_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7265330.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPRLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(10861..10937)
                     /locus_tag="JN692_003084"
     tRNA            complement(10861..10937)
                     /locus_tag="JN692_003084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10901..10903),aa:Arg,seq:tct)
     gene            11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN692_003085"
     CDS             11180..>11748
                     /gene="fimZ"
                     /locus_tag="JN692_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HAY7265331.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT         2901 a         3021 c         2999 g         2827 t
ORIGIN      
        1 taaggcggta tacaaataaa tttcgttaac ctgacgggca taattcaggt caagtaaagt
       61 ttgtcgggtt gcaaataaag aacgctcggc atccagcact tccagataac ttaccgcgcc
      121 gtgctgatat aatgcccgcg cccgttgcag ggtaatttgc agcgacgcca gataacgctg
      181 ctgggcgctg atttgatcgt ctaggctttg acgtaatgcc agcgcatctg ccacctcttt
      241 aaaggcgttc tggattttct gttcataatt caccaccgac tgctgctggc gaatttcggc
      301 gatatccaga ttggcctggt tgcgtccggc attaaaaatg gggatctcaa ttttaggaat
      361 aaaattccac atcccgctgc tagcgttaaa taatgatgac agatcgctgc tggcgtttga
      421 tattccgctg gtcaggctga tagacgggaa aaatgccgca cgcgcagcac caatattagc
      481 attagccgcc attaacgcgt gttcagcttc cataatatcc gggcgctgca ataagatttg
      541 cgacgacaag cccgccggta atttaacgct ttgcaggctg ccgctgttta ctgtctgcgc
      601 ttgcggtagc ttgccgtagc ttcccaataa cagttgcaat gcattattcg cctgcgccag
      661 ttccccctga cgtttagcga tgtcactgcg ggtactttct atcaccccgc gagcctgttc
      721 cagcgccaga acattgctgc taccggtcaa cagttgtttt tcgacaaacg catatgactg
      781 ctgataatta cgcagcgttt cttcggctat ttgcaattgc gcatacgcca gctgctgatt
      841 gaaatagctt tgcgcgacat tagaaaccag caaaatatgc accgcgcgct gggcttcctc
      901 agtggctaaa taattttggc gctcgacttc gctcatgttc tttaagcgac cgaaaaaatc
      961 gagatcaaag ctggcgttaa ggccagtcga gaactcccgc gtcgtggctg aatcgccttt
     1021 aagattgccg ctccagctgc cgctgccctc gccattgagc tgtgggtagc ggtcggcatc
     1081 ggtcagacga tattgcgccc gtgcctcctg cactttcagc gtcgccatgc gcaaatcccg
     1141 gttattcacc agcgcctcgc taatcagcgt cttcacctga ttatcaacaa aaaaggtgcg
     1201 ccagcccgcg ttcaggtagt tatccgctgc gttaaccagg ccgttctggc tgagtgagaa
     1261 ctgctgcggt acgggcattg ccggacgctg ataatccggt gccagtgaac aaccagttag
     1321 cgcaagggcc acacaaaatg gcagaagttt acaaggagac ataggctcat aatttctggt
     1381 gattttatgt cgccaacttt actcgccagg ctctgatttt ccggtgacag gaaaatgaca
     1441 aaattgtcat tttgccaata agcgattgcc atctgatccc gctactctag aattgcccgg
     1501 gcaacatgcg gaggaaatat gaaactgttg attgtcgaag atgaaaagaa aaccggagaa
     1561 tacttgacca aagggttaac cgaagccggt tttgtggtcg atttggccga caacgggctg
     1621 aatggctacc atctggcgat gaccggtgat tatgacctga taatcctcga tattatgctg
     1681 ccagacgtga acggctggga tatcgtgcgc atgttgcgct ccgccaataa agggatgccg
     1741 attctgttgc ttaccgcgct tggcaccatt gaacatcgcg tcaaggggct ggagttgggg
     1801 gcagatgact acctggtgaa accattcgct tttgctgaac tgctggcgcg ggtgcgcact
     1861 ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt ttcaggttgc cgatctgatg
     1921 gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc gcatcactct gaccagtaaa
     1981 gaatttactc tgctggagtt ttttctccgc catcagggcg aagtgctgcc ccgctcgctt
     2041 atcgcctcgc aggtatggga catgaatttc gacagcgaca ctaacgccat tgatgtggcg
     2101 gtgaagcggc tacgcggcaa aatcgacaac gactttgagc cgaagctgat tcaaaccgtg
     2161 cgcggcgtgg gttacatgct tgaggtgccg gatggtcagt aagccatttc agcgcccgtt
     2221 ttcgctggca acccgcctga ccttttttat cagcctggcc accatcgcgg cgtttttcgc
     2281 ctttgcatgg atcatgatcc actcagtaaa agtgcatttt gccgagcagg acattaatga
     2341 tttaaaagag attagcgcca cccttgaacg ggtactaaat caccctgacg aaacgcaagc
     2401 ccgacgctta atgacgctgg aagatatcgt cagtggttat tccaacgtgt tgatttccct
     2461 ggcagatagc cacggcaaaa cggtgtatca ctcccccggt gcgcttgata tccgcgagtt
     2521 tgcacgtgat gccatacccg ataaagacgc gcggggtggc gaagtgtatc tcctttccgg
     2581 cccgacgatt atgatgccag gccacggtca cgggcatatg gaacacagca actggcggat
     2641 gattaacttg ccggttggcc cgttggtgga cggcaaaccg atttatacgc tctacatcgc
     2701 gctgtcgatc gattttcatc ttcattacat aaatgatttg atgaataaac ttattatgac
     2761 cgcatcggta atcagcatcc tgatcgtctt tatcgtgctg ttggcggtac ataaaggtca
     2821 cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc acctcgaaag atctcgacgt
     2881 tcgccttgac ccgcagaccg tgccgattga gctggaacag ctggtactgt cgttcaacca
     2941 tatgatcgag cgtattgagg atgtctttac tcgccagtcc aatttctcag cggatatcgc
     3001 ccacgaaatt cgcacaccaa ttactaatct cattacgcaa acggaaatcg ccctcagcca
     3061 gtcgcgcagc cagaaggagc tggaagatgt gctctactct aatctcgaag agctgacgcg
     3121 aatggcgaaa atggtcagcg atatgctgtt tctcgctcag gccgataaca accagctaat
     3181 ccccgaaaag aaaatgctca acctggcgga tgaagtcggc aaagtgttcg attttttcga
     3241 ggcgctagcg gaagatcgcg gcgtggagtt gcggtttgtt ggcgacaaac gtcaggttgc
     3301 gggcgatccg ttgatgctgc gtcgggcgct aagcaacctg ctttctaatg ccctgcgtta
     3361 tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg gtcgatcacc aggtgcaagt
     3421 ttccgtcgaa aaccccggta cgcccattgc gcccgagcac ttaccgcgat tgtttgaccg
     3481 tttctatcgc gttgacccct cccgccagcg aaaatgtgaa ggtagcggta ttgggctggc
     3541 gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt gcggtaacgt cagatgcgcg
     3601 ggggacaagg tttgttatca cattacccgc ttaattattc agcaaacggc aacttttata
     3661 accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa agctgtcaca
     3721 attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat gtaaggtttt
     3781 tgtgtggact ggcttcttga tgtttttgct acctggctct acggcttaaa agtaatcgcg
     3841 ataacgttag cggtcatcat gttcatcagc gggctggacg atttttttat tgatgtcgtc
     3901 tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc gcgaatgagt
     3961 taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat
     4021 gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga ctacgaaaac
     4081 tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga tgttgacgaa
     4141 gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg tcccaccagc
     4201 aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg tagcgccaat
     4261 ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc gatggaattg
     4321 cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccagt gtatccgttt
     4381 gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc agaactgcat
     4441 ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc aggcgtcggc
     4501 acctgtttca gccgccgcgc cgtgaccgca ctgttagctg acggtaacgg tattgctttc
     4561 gacgtgcaga gtcttactga agattacgac attggcttcc gcctgaaaga aaaaggtatg
     4621 acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa
     4681 tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc
     4741 ttttcgactg cggttcgaca aaaatctcgc tggatcatcg gcattgtttt ccaaggcttt
     4801 aaaacccata aatggacctc cagcctgacg ctgaactact ttctctggcg cgaccgcaaa
     4861 ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgatcca gcttttgctg
     4921 ttgctggcgt atgaaagttt gtggcccgat gcctggcatt tcctttctat ttttagcggc
     4981 agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa ccgcatcgtg
     5041 cagcgagtga ttttcgtcac tggctactac ggcctcacgc agggcctgct ttccgtcctg
     5101 cgtcttttct ggggcaacct gattaacttt atggctaact ggcgcgtgct gaaacaggtt
     5161 cttcagcacg gcgatccgcg tcgcgtggcg tgggataaaa caacgcatga cttccccagc
     5221 gtcactggcg atacccgctc gttgcgcccg ttaggtcaaa ttctgctgga aaatcaggtc
     5281 atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct acgcctgggc
     5341 ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc gctggcagag
     5401 caaaacggcg tggcgtgaga atccatcgat gcctggcaga tcccgtcctc gctgattgcc
     5461 gaaatgccag cctccgtagc gctgcattat gcagtactgc cgctgcgtct ggaaaatgat
     5521 gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc cctgacgcgt
     5581 aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt cacagggtta
     5641 cgccactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta caatgcggtt
     5701 cagcatcagt ggctcacgga acagcagacc ggtgaaatct ggcggcaata tgtgccgcat
     5761 cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc agcaattaac
     5821 gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt ggtcaccgaa
     5881 ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga attacaagtt
     5941 tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc gcaactggag
     6001 tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt tactgctgac
     6061 gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag aagagctggg
     6121 gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc tgaaggcaca
     6181 gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc agcaggtgcc
     6241 ggataatatt ccgctgactt tataccttgc ggaagcctat cgtcattttg gtcatgatga
     6301 ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg cccgacttga
     6361 gcgcagtctg gcggctattc cagttgaagt gaaaagcgtt acgactgttg aagaactgct
     6421 tgcccagcaa aaagcgggcg acgctgcgcc gaccctgcgt tgtcgcagtg aagtcgggca
     6481 gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg atgcgacgtt
     6541 tgctgcatcg ccggaaggaa aaacgctgcg aaccgatctg ctgcaacggg caatctacct
     6601 gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga acacattaag
     6661 cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg acgatcggat
     6721 cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta cttacgcgac
     6781 cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcattg aaaataagcc
     6841 gctgtttacc acgggcgcac aagagaaaag ttggctctat ctgttatcta aatacagcgc
     6901 caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc gccagtatgt
     6961 tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag cgcaaaaact
     7021 gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca gcgtggcgac
     7081 ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag aaccgacaaa
     7141 tcttccccgc ctggatcaac tgacctggca actgatgcag aacgagcagt cacgcgaagc
     7201 tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca gccagacttt
     7261 aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctagcaacgc cggcgaaggt
     7321 ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa gtcagttgcc
     7381 gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt cgccttccta
     7441 cgatgctgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac ccggtgtggc
     7501 gttgtatgca tggcttcagg ccgaacaacg acaaccgagc gcctggcaac atcgtgcggt
     7561 agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct ggcagaaaat
     7621 cagtcttcac gacatgagca atgaggatct gctcgccgcc gccaacaccg ctcaggcagc
     7681 gggaaatggc gcggctcgcg atcgctggct tcaacaggca gaacaacgtg ggctgggaaa
     7741 caatgctcaa tactggtggc tgcatgcgca acgttatatc ccagctcagc cagaactcgc
     7801 actgaacgat ctcacgcgct caatcaatat tgccccttct gccaacgctt acgttgcgcg
     7861 ggcgacaatt tatcgccaac gtcataatgt tccggccgcg gtgagtgatt tgcgcgccgc
     7921 gctggaactg gaaccgaata atagcaacac ccaggcagcg cttggttacg ccttgtggga
     7981 tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag ggcttccgga
     8041 cgatccggca ctgatccgac aactggccta cgtgaaccag cgtctggatg acatgcctgc
     8101 gacgcagcac tacgcccggc tggtgattga tgacattgat aatcaggcgc tgataacccc
     8161 actgacccca gagcaaaatc agcaacgctt caatttccgc tgtttgcatg aggaggtcgg
     8221 tcgccgctgg acgttcagtt tcgattcttc catcggcttg cgttccggcg caatgagtac
     8281 cgctaacaat aatgtcggcg gtgcagcgcc tgggaaaagc tatcgtagct acggacaact
     8341 ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc tctcagttta
     8401 tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga aaaatccgat
     8461 gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc tcgccgtcga
     8521 acagcagttg ccgctgaacg ggcaaaatgg cgcatccgat accatgctgc gcgccagcgc
     8581 ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt caggctggtt
     8641 tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc aggcgtggac
     8701 ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta ttgagcctta
     8761 cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc agcttggcgg
     8821 tgtcggggtc cgctggaata tttggaccgg cgagacgcac tacgacgcct agccgcacaa
     8881 agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac gtaacggaga
     8941 gcgcaacaac gcgtttctca ccattggagt acactggtaa atgcgtaagt tcattttcgt
     9001 attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta ttatctggca
     9061 accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga tgagtcagtt
     9121 acgttttcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg atgcatttac
     9181 ccagccagaa cagcgcacgt tattgtttaa gcgtgccgca gctgcgcaac agactggcct
     9241 gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga aacagtcgtc
     9301 tgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc aagccagatt
     9361 atggagcgcc gcgcctggca taacgccgga tggctggtac atcagcgcgg aaattgacga
     9421 cctgaactgg cgcagcgaag ccgcccgtca acctttgcta acatggttaa acaacgcgca
     9481 gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt tcgccggaaa
     9541 catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaaccg gagttaatgt
     9601 ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg aacgttatct
     9661 acaggccagc gccgattgcc aaagctccgc cccagcaagc ggcattgttt atgaactgtt
     9721 tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggacgcag aaattgcctc
     9781 gctgttagcg aaacgttcct cttgcggtaa agacatgctc tatttctctc tgcgctattt
     9841 gcccgtcgcg cacggcattc tcgagtatta aatcccctcc aggtaagtcg ggtacgacct
     9901 ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa aagcatgcta
     9961 aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc atagatcctt
    10021 tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag tgaaccttgc
    10081 gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc tacgtttacc
    10141 cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga actctcaacg
    10201 ttaaatcacg tattaatcac ccacatcaac cgtaacatca tcaacgatta tctgttgttt
    10261 ttaaataaga aattaacctg tgtaaagccc tggtcgcggc tggcaacccc ggttatcgct
    10321 tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa gcagcaaccc
    10381 agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt gtctaacttt
    10441 cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag cgtccgcgac
    10501 agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg aattgtcgcc
    10561 agttcgctat gtttaagccc caggctgctc aaaaagaaat taaaaaacga aaataccagc
    10621 tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt attgatggat
    10681 aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc gtactttatc
    10741 tctgttttta aggcgtttta cagcctgaca ccgttgaatt atctcgccaa acagcgacaa
    10801 aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat gaagaagaaa
    10861 tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt tgctctatcc
    10921 aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat tatacggtca
    10981 acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga ccgcgtctga
    11041 ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg tctgaatcaa
    11101 gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa aattgtccgc
    11161 tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata ctcatcctat
    11221 catcagaatg tctattgaag ttctgttgca aaaaaacagt gaattgcaga ttgtcctgaa
    11281 aacggatgat taccgcataa ccatcgatta tctccgaacc cgtcctgttg atttaatcat
    11341 tatggatata gacttgcccg gaacagacgg ttttaccttc ctgaaaagga tcaaacaaat
    11401 ccagagcaca gtgaaagtgt tatttttatc atcgaaatca gaatgctttt atgctggcag
    11461 agcgatacaa gctggtgcta acggttttgt cagtaaatgc aatgatcaga atgatatttt
    11521 tcatgccgtt cagatgatcc tctccggata cacgtttttt cccagcgaaa cgcttaacta
    11581 tataaaaagc aataaatgta gtacgaatag ttcaacggtc actgtgctat ctaatcgtga
    11641 agtgaccata ttacgttatc tggttagcgg attatctaat aaagaaattg ccgataagtt
    11701 attgcttagc aataaaacag ttagtgcgca taaatctaat atttatgg
//
LOCUS       DADLSE010000115        11725 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLSE010000115 DADLSE010000000
VERSION     DADLSE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.115"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..2265
                     /locus_tag="JN692_003086"
                     /pseudo
     CDS             1..2265
                     /locus_tag="JN692_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2266..3915
                     /locus_tag="JN692_003087"
                     /pseudo
     CDS             2266..3915
                     /locus_tag="JN692_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            3920..4696
                     /locus_tag="JN692_003088"
     CDS             3920..4696
                     /locus_tag="JN692_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7265332.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4971..7820
                     /gene="rcsC"
                     /locus_tag="JN692_003089"
     CDS             4971..7820
                     /gene="rcsC"
                     /locus_tag="JN692_003089"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7265333.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEVQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN692_003090"
     CDS             complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN692_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7265334.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN692_003091"
     CDS             complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN692_003091"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7265335.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTLRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESDVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2843 a         2839 c         3156 g         2887 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct acgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcttgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgataa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gttgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ctggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cgggtaacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaagggttgg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaaatg tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt
     1801 ccggtgaccg ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt
     1861 actctgcagc cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     1921 acgcttacca gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     1981 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     2041 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     2101 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     2161 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     2221 agtgtcgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     2281 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     2341 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     2401 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     2461 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     2521 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     2581 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     2641 gggcaatact ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     2701 ccagaaacct cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa
     2761 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca
     2821 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     2881 gaaatccgtc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     2941 ggtagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     3001 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     3061 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccag cgtacttaat
     3121 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     3181 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     3241 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cacctgaggc agcgaaagcc
     3301 atgaccgtgg cgatacgtac ttttttacag caaaacgcca atcgggaagg tgattgcctg
     3361 accatccccg acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     3421 cgaacgatga ccgcctggac gcaggagctc atctatgcag gcgatccggt tcattatcat
     3481 ggcagtcgcg ctaccgaagg gacactttcc tagcggcaag ccacggccca ggcggggcag
     3541 ggggagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     3601 ggagctccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     3661 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     3721 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     3781 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg
     3841 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     3901 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     3961 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     4021 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     4081 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta
     4141 tgctgcggcg ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca
     4201 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     4261 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     4321 gggtacagga actattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac
     4381 cgatctcgat cttcatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     4441 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     4501 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     4561 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     4621 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     4681 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     4741 ctgctgggcg atctgctgac gccaggatat aaccattaac aagcgtcggc agattggtca
     4801 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg
     4861 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     4921 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     4981 ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag
     5041 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     5101 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     5161 aacgcacctc tgatgtgatg aaagagctta agtacatcgc cgaaaatcgc ttatcggcag
     5221 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     5281 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     5341 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     5401 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     5461 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     5521 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     5581 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     5641 gtgtcgagca gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg
     5701 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     5761 ttaagggcga tcctcgctgg atgcaggaac gctcttggtt tggctatacg gaagggttcc
     5821 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     5881 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     5941 tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc
     6001 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     6061 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     6121 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     6181 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     6241 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     6301 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     6361 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     6421 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     6481 tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     6541 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     6601 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     6661 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     6721 tgcgtttaca gcaggtcatc tccaacctgt tgagtaatgc cataaaattc accgataccg
     6781 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     6841 ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg
     6901 gaacgggcgt acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     6961 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     7021 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     7081 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     7141 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agtgcaggaa ccgactcccg
     7201 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggcaga gcggtagtga
     7261 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     7321 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     7381 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     7441 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
     7501 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
     7561 ttagtaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
     7621 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
     7681 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctct
     7741 cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca
     7801 ggaaatcgcg ggattcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
     7861 aatgccagat aagacactga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat
     7921 aatgggaatc gtaggccgga taaggcgttt acgccgcatc cggcaatcgg tgcaaatgcc
     7981 agataagaca ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact
     8041 taaggtcaca gaagagagat aattcagcag ggcgatatcg ttctcgacgc ccagcttcat
     8101 catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat
     8161 ctcggtcact aggaagcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag
     8221 acgcttgtca ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg
     8281 ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc
     8341 ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt
     8401 gttgttcata gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat
     8461 taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg
     8521 cgcatccagt ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac
     8581 attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc
     8641 atcggcaata attacgttca tattgttcat gtaataggct accttgctac agcaagctct
     8701 tgacataact gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc ttctcacgaa
     8761 tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata
     8821 cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag
     8881 tatacagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg
     8941 catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca
     9001 gttgcacttc aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga
     9061 cgcacaattg accaggccca atttcccgta cgtcagactc atcatcgctt aaaagcaagc
     9121 cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta
     9181 atctttcatc gggtgtgata caggttgcac cccaattttc taacaggcga gtgacaatat
     9241 tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt
     9301 cttcctcttc aacttccggg tcagcggcga gcattttgat atgcacagag tagcgtgtac
     9361 caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc
     9421 tcagccagaa tgccagcggg tccgcctttc cataacgatc gttttgggtc tggttgataa
     9481 acgggaagtg caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc
     9541 ggaatgtcag gcggtcttca gaggactcat cctgatcaac ctcaagggtg atttttccca
     9601 attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc
     9661 gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt
     9721 tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga
     9781 acagcaccgt ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttctt
     9841 cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga
     9901 gtttagccgc actctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt
     9961 tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt
    10021 tcttgtttac cagcacttcg cgatcctgat cgcgaataat gaaaatttgt gtgcgcggcg
    10081 cgacctggct gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa
    10141 tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcagatgc ggcagcaaat
    10201 gatcggcaat tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc
    10261 cgagcggcaa cagtgagact atctcttcat tgattgcccg taaaatgcgc aattcgttat
    10321 tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat
    10381 agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt
    10441 gcaacgtatc cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag
    10501 agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg
    10561 tcccttcttt ctcattatca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac
    10621 tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg
    10681 ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc
    10741 aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca
    10801 acatttctgc acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg
    10861 tgagatcttt aaggggtagg gttgagatca gcaccagact gttatcctga ccattcaggt
    10921 aatacatcga ccacggtaca ttttctgcac cccacaatgt atccagataa gtggacatcc
    10981 gcagagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc
    11041 ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc
    11101 cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc acgtaacgcc
    11161 aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt
    11221 tcagccaggc gttaacggca ctttgtacca ttacccccat cgtcaccagt aacacaatga
    11281 tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg
    11341 ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc
    11401 agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc
    11461 ccgtgatatt tatcatcctg tatgggggga aatagatgta caagcgccat tttgctgtac
    11521 aaataaccta caaaaagccc aacgaaatta attgcaaata aacaaagatt gctggaaatt
    11581 atgctgatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca
    11641 cagaataatg aaaagtgtgt aaagaagggt aaaaaaaaac gaatgcgagg catccggttg
    11701 aaataggggt aaacagacat tcaga
//
LOCUS       DADLSE010000116        11664 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLSE010000116 DADLSE010000000
VERSION     DADLSE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.116"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1614
                     /gene="ftsK"
                     /locus_tag="JN692_003092"
     CDS             <1..1614
                     /gene="ftsK"
                     /locus_tag="JN692_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7265336.1"
                     /translation="QPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKP
                     TTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFE
                     LNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVL
                     DNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLY
                     KAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALLWCVNEMERRYKLMS
                     ALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADL
                     MMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKI
                     DSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQY
                     VDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAAR
                     IIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            1769..2380
                     /gene="lolA"
                     /locus_tag="JN692_003093"
     CDS             1769..2380
                     /gene="lolA"
                     /locus_tag="JN692_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7265337.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            2391..3734
                     /locus_tag="JN692_003094"
     CDS             2391..3734
                     /locus_tag="JN692_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7265338.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            3825..5117
                     /gene="serS"
                     /locus_tag="JN692_003095"
     CDS             3825..5117
                     /gene="serS"
                     /locus_tag="JN692_003095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7265339.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            5357..7801
                     /gene="dmsA"
                     /locus_tag="JN692_003096"
     CDS             5357..7801
                     /gene="dmsA"
                     /locus_tag="JN692_003096"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HAY7265340.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNHTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            7812..8429
                     /gene="dmsB"
                     /locus_tag="JN692_003097"
     CDS             7812..8429
                     /gene="dmsB"
                     /locus_tag="JN692_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7265341.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            8431..9294
                     /locus_tag="JN692_003098"
     CDS             8431..9294
                     /locus_tag="JN692_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7265342.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(9330..9956)
                     /locus_tag="JN692_003099"
     CDS             complement(9330..9956)
                     /locus_tag="JN692_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7265343.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            10271..11419
                     /locus_tag="JN692_003100"
     CDS             10271..11419
                     /locus_tag="JN692_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265344.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNSSIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         2772 a         2822 c         3158 g         2912 t
ORIGIN      
        1 cagccgcaac aaccggttgc accgcagccg cagtatcagc agccacaaca gccagttgcg
       61 ccacagccgc aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg
      121 ttgcacaaac cgacgacgcc gctgccttct ttggatttgc ttacaccgcc gccgagcgaa
      181 gtggagccgg tagatacctt tgcgcttgaa caaatggcgc gtctggtgga agcgcgtctg
      241 gctgatttcc gtattaaagc cgatgtcgtt aattactctc cggggccagt tatcactcgc
      301 tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac
      361 cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc
      421 tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg
      481 gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc
      541 gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact
      601 accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca
      661 cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat
      721 gaaggcattc cgcacctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg
      781 ctgctctggt gtgttaacga gatggagcgg cggtataaac tgatgtctgc gctgggtgtg
      841 cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt
      901 ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa
      961 gaaccataca ttgtcgtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa
     1021 aaagtggaag agctgatagc acgtctggcg caaaaagccc gtgccgcggg tatccacctc
     1081 gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt
     1141 ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag
     1201 gctggcgcgg aatcactgct gggtatgggg gatatgctct actctgggcc gaactccacg
     1261 ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag
     1321 gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacctccga cagcgaaagc
     1381 gaaggtggtg cgggtggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg
     1441 gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc
     1501 attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc
     1561 gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg
     1621 cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta
     1681 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat
     1741 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact
     1801 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
     1861 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
     1921 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
     1981 tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
     2041 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
     2101 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
     2161 cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
     2221 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
     2281 cagttatcag ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
     2341 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
     2401 tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa
     2461 atttagcaca gtatatcggt cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg
     2521 ctatcgaagc cgggcatttg cattctatga tcctctgggg gccaccgggt accggcaaaa
     2581 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
     2641 tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg
     2701 caggtcgccg cactattctt tttgttgacg aagttcaccg tttcaacaaa agccagcagg
     2761 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
     2821 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtttat ctgttgaaat
     2881 ccctgagtac agaggatatt gagcaagtac taactcaggc gatggaagac aaaacccgtg
     2941 gctatggtgg tcaggatatt gttctgccag atgatacacg acgtgccatt gcagaactgg
     3001 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
     3061 tctatgatag cggtaagcgg gtcctgaagc ctgaattact gaccgaaatc gccggtgaac
     3121 gtagcgcccg ctttgataac aaaggcgatc gcttttacga tctaatttcc gcactgcata
     3181 agtcggtacg tggtagcgca cccgatgcgg cgctgtactg gtatgcgcga attattaccg
     3241 ctggtggcga tccgttatat gtcgcgcgtc gctgtctggc gattgcgtct gaagacgtcg
     3301 gtaatgccga tccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
     3361 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
     3421 caaaaagcaa cgctgtctac actgcgttta aagccgcgct ggccgatgct cgcgaacgcc
     3481 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
     3541 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
     3601 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
     3661 aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
     3721 aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg
     3781 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
     3841 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
     3901 atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa
     3961 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
     4021 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
     4081 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc
     4141 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgttgaa gtcagccgct
     4201 ggggtacccc gcgtgagttt gactttgaag ttcgtgacca cgtgacgctg ggtgaaatgc
     4261 actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga
     4321 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
     4381 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
     4441 gtacgggtca gctgccgaaa tttgctggcg atctgttcca tactcgtccg ctggaagaag
     4501 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg
     4561 tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat
     4621 gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc
     4681 agttcgacaa agttgaaatg gtgcagattg tgcgcccaga agactcaatg gcggcgctgg
     4741 aagagatgac tggtcatgca gaaaaagtcc tgcagttgct gggcctgccg taccgtaaaa
     4801 tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac ttggaagtat
     4861 ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc
     4921 aggcacgtcg tatgcaggca cgttgccgca gcaagtcgga caagaaaacc cgtctggttc
     4981 ataccctgaa cggttctggt ctggctgttg gtcgtacgct ggttgcagta atggaaaact
     5041 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac
     5101 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgccttt agggcgcttt
     5161 ttttgtctcc ctttgatacc gaacaataat tactcctcac ttacacgtaa tactactttc
     5221 gagtgaaaat ctacctatct ctttgatttt caaatcattc gatgtataca agcctatata
     5281 gcgagctgct atagaaataa ttacacaata cggtttgtta ctggaatcaa tcgtgatcaa
     5341 gcttgagtga gccattatga aaacgaaaat ccctgatgcg gtattggctg ctgaggtgag
     5401 tcgccgtggt ttggtaaaaa cgacagcgat cggcggcctg gcaatggcca gcagcgcatt
     5461 aacattacct tttagtcgga ttgcgcacgc tgtcgatagc gccattccaa caaaatcaga
     5521 cgaaaaggtt atctggagcg cctgtacagt taactgtggt agtcgctgcc cgctacgtat
     5581 gcacgtcgtg gacggtgaaa tcaaatatgt cgaaacggac aataccggcg atgacaatta
     5641 cgacggcctg caccaggttc gcgcctgcct gcgtgggcgt tccatgcgtc gccgtgtcta
     5701 caatccggac cgcctgaaat atccgatgaa acgagtcggg gcgcgcggtg aaggcaaatt
     5761 cgagcgcatt agctgggaag aagcctacga catcatcgcg accaatatgc agcgcctgat
     5821 caaagagtac ggcaacgagt ctatctatct gaactatggc accggtacgc tgggcggcac
     5881 catgacccgc tcctggccgc cgggaaatac cctggtcgcg cgcctgatga actgctgcgg
     5941 cggttatctt aaccattacg gtgactactc ttccgcgcaa attgcggaag gtttgaacca
     6001 tacctacggc ggctgggcag atggtaacag cccgtcggat atcgaaaaca gtaaattggt
     6061 agtgctgttt ggtaataacc caggcgaaac gcgaatgagt ggcggtgggg tgacttacta
     6121 tcttgaacag gcacgccaga aatctaatgc tcgcatgatc atcatcgatc cgcgctatac
     6181 cgacaccggt gccgggcgcg aagatgagtg gatccctatt cgtccaggaa cagatgccgc
     6241 actggttaac ggtctggcgt acgtcatgat cactgaaaac ctggtggatc aggcattcct
     6301 cgataaatat tgcgttggct atgacgagaa aaccctgccc gccagtgcgc cgaaaaatgg
     6361 tcattataaa gcttatattc tgggtgaagg gccagatggc gtggctaaaa caccggaatg
     6421 ggcctcgcaa atcaccggtg ttccggcaga caaaatcatc aaactggctc gtgaaatcgg
     6481 cagtaccaaa ccggcgttta tcagccaggg atggggcccg cagcgtcacg ctaacggtga
     6541 aatcgcaacc cgtgctatct cgatgctggc gattctgacc ggtaacgttg gtattaatgg
     6601 agggaacagc ggcgcgcgtg aaggttcata cagcttaccg tttgtccgta tgccgacctt
     6661 ggaaaacccg atccagacca gcatttcgat gtttatgtgg accgatgcca ttgaacgtgg
     6721 cccggaaatg acggcgctgc gtgatggtgt gcgcgggaaa gataagctgg atgtgccgat
     6781 caaaatgatc tggaactatg ccggtaactg cctgattaac cagcattctg aaatcaaccg
     6841 tacccatgaa atcctccagg atgataagaa gtgcgagctg attgtggtta tcgactgcca
     6901 catgacctca tcggcgaaat atgctgacat cctgctgcct gactgcaccg cttccgaaca
     6961 gatggacttt gcactggatg catcctgcgg gaatatgtct tacgtgattt tcaacgatca
     7021 ggtgattaaa ccgcgctttg aatgtaagac catctatgaa atgaccagcg aactggcaaa
     7081 acgtcttggc gttgagcaac agtttactga aggccgtacc caggaagagt ggatgcggca
     7141 tctgtatgcc cagtcgcggg aagcgattcc tgaactgcca acgtttgaag agttccgcaa
     7201 gcaggggatc tttaaaaagc gcgacccaca agggcatcac gttgcttata aagccttccg
     7261 tgaagatccg caggcaaacc cactgactac gccatcgggc aaaattgaga tttattcgca
     7321 ggcgctggct gacattgccg ctacctggga attgccagaa ggcgatgtga tcgatccact
     7381 gccgatctac acgccgggct ttgaaagtta tcaggatccg ctgaacaaac agtatccgct
     7441 gcagcttaca ggtttccact ataaatctcg cgttcactca acttacggca acgttgatgt
     7501 gctgaaagcg gcttgccgtc aggaaatgtg gatcaacccg cttgatgccc aaaaacgcgg
     7561 tatccacaac ggcgataaag tcaggatctt taacgatcgt ggtgaggttc atattgaggc
     7621 gaaagtgacg ccacgaatga tgccgggcgt ggtcgcactg ggtgaaggcg cctggtatga
     7681 cccggatgca aaacgtgtcg ataagggcgg ttgtatcaac gtactgacca ctcaacgtcc
     7741 gtctcctctc gctaagggga atccgtcaca tacaaacctt gttcaggttg aaaaggtgta
     7801 aggagtaacc gatgacaacc cagtatggat tttttattga ttccagccgt tgcaccggtt
     7861 gcaaaacctg cgagctggct tgtaaagact acaaagattt gacgccagaa gtcagtttcc
     7921 gccgcattta tgaatatgct ggcggcgact ggcaggaaga taacggtgtc tggcaccaga
     7981 acgtgtttgc ctactatctg tcgatttcgt gcaaccactg cgaagatccg gcttgtacta
     8041 aagtctgccc gagcggtgcg atgcataaac gcgaagatgg ttttgttgtg gtcgatgaag
     8101 atgtatgcat tggctgccgt tactgtcata tggcttgccc gtatggcgca ccgcaatata
     8161 acgaaacgaa aggccatatg accaaatgcg atggttgtta tgaccgtgtt gcggagggta
     8221 aaaagccgat ctgtgttgaa tcttgcccgc tgcgggcgct ggatttcggc cctatcgacg
     8281 agctgcgtaa aaaacatggc gatctggcgg ccgttgcgcc gttgccgcga gctcacttta
     8341 ccaaaccgaa tattgtgatc aaacccaatg ccaatagccg cccgaccggg gataccactg
     8401 gctatctggc aaacccgaag gaggtgtgag atgggaagtg gatggcatga atggccgctg
     8461 atgatcttca cggtcttcgg gcaatgtgtg gcgggtggtt ttatcgttct ggctttggcg
     8521 ctgctcaaag gcgacctgcg agcagaagcc cagcagcgtg ttatcgcctg catgtttggc
     8581 ttatgggtac tgatgggcat tggctttatc gcctctatgc tccatcttgg ttcaccgatg
     8641 cgcgccttta actcgctcaa ccgggtaggg gcttcagcac tcagtaacga aatcgccagc
     8701 ggttcgatct tttttgctgt aggcggcatc ggctggttgc tggcaatgct gaaaaagctg
     8761 tcaccggcat tgcgtacgct atggctggta gtgacgatgg ttcttggcgt cgtctttgtc
     8821 tggatgatgg tgcgtgtgta taacagcatt gataccgttc cgacctggta cagcatctgg
     8881 acgccgatgg gcttcttcct gacgatgttc atgggcggcc cgttactggg ttacctgttg
     8941 ctgagtctgg ctggcgtcga tggctgggcg atgcgtctgc tgccagccat ttctgtactg
     9001 gcactggtgg tgagtggcgt gatgtcagtg atgcagggca cagagctggc aactattcat
     9061 agttctgtgc agcaggccgc agcgctggtg ccggactatg gtgcgctgat gtcctggcgg
     9121 atcgtgcttt tggccgttgc cctgtgcttg tggattgcac cacagctaaa aggttatcag
     9181 cctgcggttc cgctactatc cgtctcattc attctgcttc tggcagggga attaatcggt
     9241 cgcggcgtat tctatggttt gcatatgacc gtggggatgg ccgtcgcaag ctaatcagta
     9301 acaaccgggg tttcggcccc ggttctcttt tatttctgct tcgttaacgt gtcataactg
     9361 gtcatcaaat tacgataatc aggaatatgg ttagagaaca acgtcgccag tccttcaata
     9421 tcattacgcc agtcacggtg cagctcgcaa gccacgccga accatgtcat caattgcgca
     9481 ccagcttgcg acatgcgatc ccacgccgaa tgccgggtaa tttcattaaa ggtccctgat
     9541 gcatcagtca cgacaaagac atcaaaccct tcttcaatgg ctgaaagcgc cgggaatgcc
     9601 acgcaaactt cggttaccac accggcaata attaactgtt ttttacctgt cgctttgact
     9661 gcttttacaa aatcttcgtt atcccaggcg ttaatattgc ccgggcgagc aatgtaaggt
     9721 gtatcgggga attgtgcttt cagttctgga actaatgggc cgttaggacc ggtttcaaaa
     9781 ctggtggtaa gaatagttgg taagttgaaa tacttagcca ggtcacccag cgccagcacg
     9841 ttatttttaa acttatcggg ttcgatatcc cgtacaaggg aaagtaaacc agcctggtga
     9901 tcaacaagca aaacggcagc atcattttta tcaagacgaa catacggttt ggtcatcctc
     9961 ttctcctttc gagagtcagt ggtacgtctg aaatcggtca gacgcgatgc attgctctga
    10021 aagcatagac gggaaatatg agtttgctgt gaccatgaaa tttttcgact gaacgaagcg
    10081 tatgaaattt gtgttagatc aataaaaaca atcagatgag attaatcatc tcttcagcat
    10141 tagcgttatg aatgagtaat ataaatcgat aataccgccg ctgcgatatg cgcgtatttt
    10201 cacccattga caatgttttt ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg
    10261 gttttaattc atgtccacgt atacccggcc tgtcatgctt ttgctgtctg gcctgctttt
    10321 gttgactctg gcgatcgcgg tgttaaatac actcgtgccg ctttggctcg cccaggaaca
    10381 catgtccaca tggcaggtag gcgttgtcag ctcatcctat tttaccggca accttgtcgg
    10441 tacattgctg acagggtatg tcattaaacg cattggcttt aaccgcagct attatctgtc
    10501 ctccttcatt tttgctgctg gctgtgccgg ccttggcctg atgattggtt tctggagctg
    10561 gttggcgtgg cgttttgtcg cgggcgtcgg ctgtgccatg atttgggtgg ttgttgagag
    10621 cgcgctgatg tgcagtggga cgtcacgtaa ccgtgggcgt ttgcttgctg cgtatatgat
    10681 ggtttattac gtgggaacgt ttttaggcca gttactggtc agcaaagtct caaccgagct
    10741 gatgagcgta ttgccgtggg ttacaggttt gacattggca gggatcttac cgctgttgtt
    10801 tacgcgtgtc ctgaatcagc aggctgaaaa ccatgattcg acgtcaatta cggcaatgct
    10861 aaaactccgt caggcgcggc ttggcgtgaa tggctgcatt atctcaggaa tcgttctggg
    10921 atctctatat ggcctgatgc ctctgtacct caatcacaaa ggggtgagca attccagtat
    10981 tggtttctgg atggcggtac tggtcagtgc gggtatcctc ggacaatggc cgattggacg
    11041 tctggcggat aagtttggtc gactgctggt tttgcgtgtt caggtctttg tcgtcattct
    11101 cggcagtatt gcgatgctta gccaggcggc gatggcccca gcgttattca tcctcggtgc
    11161 cgctggcttt acgctatatc cggtggcgat ggcctgggct tgtgagaaag ttgaacatca
    11221 tcaactggtg gcgatgaacc aagccttact gttgagctat accgtgggaa gtctgcttgg
    11281 cccgtcattt accgctatgc taatgcagaa tttctccgat aatttattgt ttatcatgat
    11341 cgccagcgta tcgtttatct atttgctgat gctgcttcgc aacgccggtc atacgccgaa
    11401 acccgttgct cacgtgtaaa tgaattcaag caaagtgtga acttactgtt tcacactctg
    11461 cttttttgtt tcttctatct gacttgcttt tttccaaatt ttattcgttt aaaaataaaa
    11521 tgtgcagcag gttataattt tgcatttcgc tattaccgca cttcttattt gccgcgcata
    11581 atccctcgtt ttacagatgc ccctttaatt ttggcgaagg atttgtctat ggctgggaat
    11641 gttcaggaaa aacagttgcg atgg
//
LOCUS       DADLSE010000117        11582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLSE010000117 DADLSE010000000
VERSION     DADLSE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.117"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1176
                     /locus_tag="JN692_003101"
     CDS             <1..1176
                     /locus_tag="JN692_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265345.1"
                     /translation="RLNQQHNEFMMVAKWCEWAIQQDGLQENLQKNLYEILEENQQNK
                     QSEIPQEDLKESLEEIKENILEENLPASRIENRAEALRRMKECLITRRSMLNLSNLGL
                     TSLPENLPPHLIEFYCSKNVLTALPKVMPKWLLVLDCTDNVLILLPKVQPSKLMVLKC
                     YENCIIWLPELSTNLRVINCSENFLQFLPPSMPQYLYKLSCAGNNINSIPDEMLENLT
                     RLKVFDCSSNDLISSPRLPPKLIIYYCGENKFKTVQVPQPQSLKVFDCNGNPWDKDNL
                     PTLLKAVEGLKKQQGLKDLLDFLHKEGQVDLEGLEELEDLMDLEFLDDPELLERVKVQ
                     EDLELLDQQLGLLSLERQQDSQPVNQQSEHEPESASRVKRDLSEVDSESTMKRKRFM"
     gene            complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN692_003102"
     CDS             complement(1344..2414)
                     /gene="dadX"
                     /locus_tag="JN692_003102"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7265346.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     LRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2424..3722)
                     /locus_tag="JN692_003103"
     CDS             complement(2424..3722)
                     /locus_tag="JN692_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7265347.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLVRMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFCDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            4052..5584
                     /locus_tag="JN692_003104"
     CDS             4052..5584
                     /locus_tag="JN692_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7265348.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFLPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVQDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN692_003105"
     CDS             complement(5636..6355)
                     /gene="fadR"
                     /locus_tag="JN692_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7265349.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            6576..8117
                     /gene="nhaB"
                     /locus_tag="JN692_003106"
     CDS             6576..8117
                     /gene="nhaB"
                     /locus_tag="JN692_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7265350.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            8263..8793
                     /gene="dsbB"
                     /locus_tag="JN692_003107"
     CDS             8263..8793
                     /gene="dsbB"
                     /locus_tag="JN692_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7265351.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(8839..10107)
                     /locus_tag="JN692_003108"
     CDS             complement(8839..10107)
                     /locus_tag="JN692_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7265352.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMFSPRYTTRSSDLLRVK"
     gene            complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN692_003109"
     CDS             complement(10107..10526)
                     /gene="umuD"
                     /locus_tag="JN692_003109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7265353.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            10899..11582
                     /gene="hlyE"
                     /locus_tag="JN692_003110"
                     /pseudo
     CDS             10899..11582
                     /gene="hlyE"
                     /locus_tag="JN692_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309704.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
BASE COUNT         2898 a         2976 c         2822 g         2886 t
ORIGIN      
        1 cgcctgaatc agcaacataa cgagttcatg atggtcgcca aatggtgcga atgggctata
       61 cagcaggatg gtttgcaaga aaacctgcaa aaaaacctgt acgaaatcct ggaagaaaac
      121 cagcaaaaca aacagtcaga aattccccaa gaagacctga aagaaagtct ggaagaaatc
      181 aaggaaaata tcctggaaga aaacctgcca gcaagccgga ttgaaaaccg ggcagaagca
      241 ttgcgcagaa tgaaggaatg tctcattaca agacggagta tgctcaatct tagcaacctg
      301 ggattgactt cactccctga gaatttgcct ccacatctga ttgaatttta ctgcagtaaa
      361 aacgtgttga ccgcgttacc gaaggtgatg ccaaagtggc tgctggtgct tgactgtaca
      421 gataacgtct tgatcttgtt accgaaggtg cagccctcaa aactgatggt actgaagtgc
      481 tatgaaaact gtattatctg gctgcctgag ctgtcgacta acctgagagt gattaactgt
      541 tctgaaaact tcttgcaatt tttaccgccg tcgatgcccc agtacctgta taaactatcc
      601 tgtgccggca acaatattaa ctcaatacct gatgagatgc tggagaactt gactcgcctg
      661 aaagtatttg actgttctag taacgatttg atctcttcac cacggctgcc gcccaaacta
      721 ataatttact actgtggaga aaacaagttt aaaacggtac aggtgccgca gccccagagc
      781 ctgaaggtgt ttgactgtaa tggtaacccg tgggacaaag acaatttacc gacgctgctc
      841 aaagccgtcg agggcctgaa aaaacagcag ggcctgaagg accttttgga ctttttgcac
      901 aaggaaggcc aggttgacct ggaaggactc gaggagctgg aggaccttat ggatctggag
      961 ttcctggatg acccggaact cctggagcgc gtgaaggtac aggaggacct ggagctcctg
     1021 gatcaacagt tgggcctgtt gagtctggaa agacaacagg actcgcagcc tgttaatcaa
     1081 caatctgaac atgaacccga atctgcatca agggtgaagc gtgatttatc tgaagtcgac
     1141 tccgagtcaa caatgaagcg taagcgtttt atgtagaacg cctgtgcggt gagttcaggt
     1201 tccggggaaa tagtgtccat tcttttaatg gacactatca tgggcaatgg ctctgtcaca
     1261 ttaacaacta cagttgctga ccagccggat ggatgatttt ttcgcaacca gaagacgctg
     1321 cctccgatcc ggcttacaac aagttacact gtcacaaccg ggacgcgtaa cgccagcgcg
     1381 cacatcaact catagcccac cgttccggca gcggcggcga catcatcaat tttgatctcc
     1441 ttgccccaca gctcaaccgg cgtaccaatc cccgcctgcg ggcaaggcgt taaatcaacc
     1501 gccagcatat ccatcgagac ggtccccacc gtcatggtgc gcacgccgtc cactaaaaca
     1561 ggagtaccgg taggcgcatg gcgcagataa ccgtcggcgt atcctgcggc gacaatgccg
     1621 attcgctgtt catcgcgcgc agtatagcga ccgccgtagc ccacacgctc gcccgctttt
     1681 agcgtctgga caccaataat ctcactgctt agcgtcatca ccggacgtaa tccggtattg
     1741 gcgatatcac gccactgacc ggacggcgaa gcgccataca aaataatgcc aggccgaacc
     1801 cagtcaaaat gcgcttccgg gtgccacaga gtcgccgccg aattggacaa cgaacgccga
     1861 cactccagcc cctccgccgc ctgctcaata cgcgccatcg cgccggaaat tccatcagga
     1921 tgttccgctt cagcaaaatg cgacatcagg gtcatttcgc caacattcgc cattgcccgc
     1981 aactgctgcc agacggtaag cacgcgatcg ggctggaagc ccaaccgatt catcccactg
     2041 ttcactttaa gataaatatc caacggtgct tttagccgcg cattttgcag tgctttgagc
     2101 tgccagttgc tgtgtacgca ggtggtcagg cggtgctggt cataaatctc cagatcctga
     2161 gcatggaaaa atccttccag catcaggatc ggccccttcc agccgcgctc acgtaacgtt
     2221 attgcctctt ccaggttaag taatgcaaag ccatcggtgg ccccgagcgc gctccagata
     2281 cgctcaatac catgcccgta agcgttcgct tttaccaccg accagacgcg cgcgtgcggc
     2341 gcggcctggc ggacaatgga cagattctgt tttaatgcct gcagatcgag gctggcctgt
     2401 atcggacggg tcatctcgtt tccttagctg tgtgcgccat gtaaatggcc cggacgtgat
     2461 ggcgtaaatc cacggctgta gcgcgctacg cttagatcct catatgggat cgctggcgtg
     2521 cgaccagaga gcagatcgct taacaactga ccggaaccgc aagccatcgt ccagccgagc
     2581 gtgccgtgac cggtattcag ccacagattt ttaaagcgtg tacgcccgac aaccggcgta
     2641 ccgtctggcg tcatcgggcg caaacccgtc cagaaagtcg cctgctcgac atgaccgccg
     2701 cgtggataga gatcgcgaac caccatttcc agcgtttcac gacgcggttg caacagctcg
     2761 gtgttaaaac caacaatttc cgccattccg ccaacacgaa tgcggttatc gaaacgagta
     2821 atggcgattt tgtaggtttc atcaagaatg gtggatactg gcgcaccatc ttcctgcgca
     2881 attggaatag tcagcgagta gcctttcagc ggatacaccg gaatatcaac aatgcctttg
     2941 agcatcgccg tcgagtaaga accaaacgcc atcacatacg catcggcctt aatcacttca
     3001 tcgcaaaact tcacgccgta gatttgctcg ccgtcgcaaa gcagttggtc aacgggcgta
     3061 ttaaagcgga atttaacccc cgcctgctct gccatccgca ccagattctg ggtaaacagc
     3121 tggcagtctc cggtttcatc attgggtaac tgcaggccgc ccgtcagttt gtgcgctact
     3181 tctgccagcg cgggatccac ttccgccagg cggctggatt ccagcagctg atacggtacg
     3241 ccggcatctt ccagcacggc gatatcgcgg gtcgcatttt catactgttg ttcggtacgg
     3301 aacagttgca gcgtcccacc ctgacgccct tcatactgaa tattggtttc ggcgcgcaat
     3361 gctttcaggc aatcacggct gtattccgcc agacgcacca tccgcccttt gttttccatg
     3421 tagtggctgg tgtcgcagtt acgtaacatt tgccacatcc atttcaactg aaactgcgta
     3481 ccgtcgagac gaaccgccag cggcgcatgg cgctggaaca tccatttaat cgcttttaaa
     3541 ggcacacctg gtgctgccca cggtgcagca tatccggggg agatttgccc ggcattcgcg
     3601 gcactggttt ccagggcggc ccccggctcc cgatcaatga cggtgacctc atgtcctgcc
     3661 tgatttaagt accaggcgct ggcaacgcct accacaccac ttcccagtat gacaactcgc
     3721 atagccactc cgataacggt agatacagta aaagaataat aatctaatta catcttgata
     3781 actcagttga aaatattatt caacatacgg cttttttatg gtgagctgac tcacatctcc
     3841 ctgactctgc ggcaatatgg cgtctttagt atctcctgct gaacgccctt tttatccagc
     3901 ataaaattcc gttcagaagc ggattagtgg cactctgata ataagaaatc gcaaagaaaa
     3961 aaattctccg gcgacgcggt ttttaacagg tgttctatgc ttgaaatgag gtgctccaca
     4021 cagagagcgc cgacaacaat gaggtgcgcg tatggcgacg atcgattcta tgaataagga
     4081 caccacacgt ttgagcgatg gacccgactg gacgttcgac ctgctggatg tttatctggc
     4141 agagatagac cgggtggcga aactctaccg gctggatacc tacccgcacc agattgaagt
     4201 gataacctca gaacagatga tggatgccta ttccagcgtc ggcatgccaa ttaactatcc
     4261 gcactggtca ttcggtaaaa agtttatcga gactgaacgg ctgtataagc acggtcagca
     4321 agggctggcc tatgaaatcg tcattaactc taacccgtgt atcgcttact tgatggaaga
     4381 gaacaccatt accatgcagg cgctggtgat ggcacacgcc tgctatgggc ataactcttt
     4441 ctttaaaaac aattgcttat tccgcagttg gaccgacgcc agttcgattg tcgattatct
     4501 gatttttgcc cgtaaatata ttaccgagtg cgaagaacgt tatggcgttg atgaagtaga
     4561 acggcttctg gactcgtgcc acgcgctgat gaactacggc gtggaccggt acaaacgccc
     4621 gcaaaaaatc tcgctgcaag aagagaaagc ccggcagaaa agtcgcgaag agtatctaca
     4681 aagtcaggtc aatatgctct ggcgtaccct gccgaagcgc gaggaagaga aaacggttgc
     4741 tgaagcgcgc cgctatccgt ccgaaccaca agaaaacctg ctctatttta tggagaaaaa
     4801 tgcgccactg ctggaatcat ggcagcgtga aatcctgcgt attgtgcgta aggtgagcca
     4861 gtatttttat ccgcaaaaac agactcaggt gatgaacgaa ggctgggcga ccttctggca
     4921 ctataccatc cttaaccatc tgtatgatga agggaaagta acggaacgtt ttatgctgga
     4981 gtttttgcac agccacacca atgtggtctt cctgcccccc tataacagcc cgtggtacag
     5041 cggcatcaac ccgtatgccc tcgggttcgc catgttccag gatattaaac ggatttgtca
     5101 gtcgccaacg gaagaagaca aatactggtt cccggatatc gccggttccg actggctgga
     5161 aacgctgcat ttcgcgatgc gtgatttcaa agatgagagt tttatcagcc agtttctgtc
     5221 accgaaagtg atgcgtgatt tccgcttctt caccgtgcag gatgatgatc ggcataatta
     5281 tctggagatt tccgctattc ataatgaaga aggttatcgg gagatccgta accggttatc
     5341 gtcgcaatat aacttaagta atctggagcc gaatattcag atctggaacg tggatttgcg
     5401 cggcgaccgt tcgctgacgc tgcgttatat tccacataat cgcgcaccgc tggatcgggg
     5461 gcgcaaagaa gtcctgaagc atgtgcatcg cctgtgggga tttgatgtga tgctcgaaca
     5521 gcaaaacgaa gacggcagca tcgagttgct ggaacgttgc ccgccaagaa tgggaaatct
     5581 gtaaaaacaa caaaaaaccc ctcgtttgag gggtttgctc tttaaacgga agggattatc
     5641 gcccctgaat ggctaaatca cccggcagat ttttctgcat ccggtgccaa atctcgccac
     5701 tctcatgccc atagcgacgc actgtttcgt acacctgatc gtgcgcgcct tcactgcaca
     5761 acgccgacag tttgtggtag aagcccagcg ccagactgcg cgcttccgga ttggcgaaat
     5821 agtgacgacc aatgcgcgta tacagccctt tcatcccgtt aaggatcaga ccgtaaatcg
     5881 ggttgccgga agcaaacgcc aggccgcgga atatgttgta atccagctcg gcaaaggcat
     5941 cggcgtgatc ggccacttca ttagcggtag ccagcacttc ctgcgcttta tcgggatgct
     6001 gacgaaacgc ggtgcgaata aaaatagtgg aaatattggt acgcaccgac agcaaattat
     6061 caataagctg cggcacactt tcgtgatcca gtcgcgccag tgtttcaagg atatttaaac
     6121 cggaagtttc ccagaaatta ttcaccttcg tcggcttgcc atgttgaatg gtcaaccagc
     6181 catctcgtgc cagacgctgt aacacttcac gtaacgtagt acgcgttacg ccaattaatt
     6241 ctgaaagttc acgttctgcg ggcaaaatag tcccgggagg gaagcggtta ttccagatac
     6301 tttcaataat gtactcttcc gcgaaacccg ccgggctttg cgccttaatg accatagtga
     6361 gatttccata acacagcaaa acaaagttgg actcatcata ccagagggct aactacgctg
     6421 atagcagaca aaaagaagaa aaagggatca aggcttcatt ttccgcatct ctttcccctt
     6481 gactatcaag acttgcctca ctcctgcctg accgttaagc tttgcgttta aacataaaaa
     6541 catcattgat tatttgtaag gtaagggaaa ccatcatgga gatctcctgg ggccgcgcgc
     6601 tatggcgcaa ttttttgggc cagtcccccg actggtacaa actcgccctc atcattttct
     6661 taatcgtaaa cccgttaatt ttcctcatca gccctttcgt cgcgggttgg ttgctggtag
     6721 cggaatttat tttcactctg gcgatggccc tgaaatgcta cccgctactc cccggtggtc
     6781 tgttggctat cgaagcggta ttcatcggca tgaccagcgc ggaacacgtc cgtgaagagg
     6841 tggcggcaaa tcttgaagtc ttgctgttat tgatgtttat ggtggcgggt atctatttta
     6901 tgaaacagct gttgctgttc atatttaccc gtttgctgtt aagcattcgc tccaaaatgc
     6961 tgctgtcgct ctctttttgc gtggcggcag cgttcctctc cgcgttcctc gatgccttaa
     7021 ccgtcgtggc ggtggtgatc agcgttgcag tcggttttta tggtatttat catcgcgtag
     7081 cctcttcccg taccgaagac accgacctgc aagacgatag tcatatcgac aagcattaca
     7141 aagtggttct ggaacagttc cgtggctttc tgcgtagcct gatgatgcat gccggtgtcg
     7201 gcaccgcatt aggcggcgta atgaccatgg tgggcgaacc acagaacctg atcatcgcta
     7261 aagcggctgg ctggcatttt ggcgatttct tcctgcgcat gtcgccggtg accgttccgg
     7321 ttctgatttg tggcctgtta acctgcctgc tggtagagaa gctgcgttgg tttggctacg
     7381 gtgaaacgct gccggagaaa gtccgcgaag tgttgcaaca gtttgacgat caaagccgcc
     7441 accagcgtac ccgtcaggat aaaatccgtc tgattgtcca ggcgattatt ggcgtctggc
     7501 tggtgactgc gctggcgttg catctggcgg aagttggctt gattggtttg tcagtcatta
     7561 ttctggcaac atcattgacc ggtgtcaccg atgaacatgc tatcggtaaa gccttcaccg
     7621 aatctctgcc attcaccgca ctgttgacgg tgtttttctc ggtagtcgcg gtgattatcg
     7681 accaacaact gttttcgccg attattcagt ttgtgttgca ggcatctgaa catgctcagc
     7741 tgtcgctgtt ctatattttc aacggtctgc tgtcatccat ttcggataac gtcttcgtgg
     7801 ggacgattta tatcaacgaa gcgaaagcgg caatggaaag tggcgctatc acgttgaagc
     7861 aatacgagct gctggcggtc gccattaata ctggtaccaa tctgccctct gtcgctacgc
     7921 cgaacggtca ggctgcgttc ctgttcctgc tgacctctgc actcgcgcca ttgattcgcc
     7981 tctcttatgg ccgcatggtg tggatggccc tgccttacac cctcgtactg acactcgtcg
     8041 gcttgctctg cgtcgagttt acgcttgccc ctgtaaccga atggtttatg caaatgggct
     8101 ggatagcaac gctttgataa caacttaccg ggcatataaa tgcccggttt gccttttcgc
     8161 ccgataattg tccaattgcg tcctattttt gctgcctcct ggtggcgcag cgaatgaatt
     8221 ggtttaaact gcgcactcta tgcatattgc agggaaatga ttatgttgcg atttttgaac
     8281 caatgttcac aaggccgggg cgcgtggctg ttgatggcgt ttactgctct ggcactggaa
     8341 ctgacggcgc tgtggttcca gcatgtgatg ttactgaaac cttgcgtgct ctgtatttat
     8401 gaacgctgcg cgttattcgg cgttctgggt gctgcgctga ttggcgcgat cgccccgaaa
     8461 actccgctgc gttatgtagc gatggttatc tggttgtata gtgcgttccg cggtgtgcag
     8521 ttaacttacg agcacaccat gcttcagctc tatccttcgc catttgccac ctgtgatttt
     8581 atggttcgtt tcccggaatg gctgccgctg gataaatggg tgccgcaagt gtttgtcgcc
     8641 tctggcgatt gcgccgagcg tcagtgggat tttttaggta tggaaatgcc gcagtggctg
     8701 ctcggtattt ttatcgctta cctgattgtc gcggtgctgg tggtgatttc ccagccgttt
     8761 aaagcgaaaa aacgtgatct gttcggtcgc taatccattc ggcgctcctg cgggagcgct
     8821 tttttcctgc cgctatattt atttgacccg cagtaaatca gaacttcgcg ttgtataacg
     8881 tggtgaaaac atagctcgct tcatctgcca ttgttgttgg atcccctgcc cggcaaaata
     8941 gagtgttcct ctgccctctt tagcattcag agtatccatt accgccatca attgctcact
     9001 cccggggcgc ggtgcgttgt catcgaataa attgagctgc gcgactccct gactgaagaa
     9061 atcccccagc atcacgcccg ctttttggta gcgatggccc gcttgccaga tggcatccag
     9121 agatcgcgta gcagcgttaa tgatatccct gctgtcctga gtgggcgtca gcagttttac
     9181 cgacgcgcta ttgccgtaat aaggttcatt gagcgcaaat ggtgacgtct taataaatgt
     9241 ggagataaac cgacaatatt gatgctcact gcgaagtttt tccgccgccc gggcagcgta
     9301 actacaaatg gcctgccgca tcgacgtata atccgtgatg cgttcaccaa acgagcggga
     9361 acagataatt tcctgcttcg tcggtgcaaa ctcttccagt tgcaaacagg gttcgccgcg
     9421 cagttcacgc accgttcttt cgagcatgac attaaaatgt ttacggataa accggatatc
     9481 tgtatccgcc aaatcgagaa cggttttgat ccccatcgcg tccagttttt tgctgatccg
     9541 ccgtccaatc ccccagacgt catccacggg gagagcagac attaatttac gctggcgttc
     9601 cagatttgat aaatccacca ccccacccgt ctgccgctgc catttttttg ccgcatgatt
     9661 ggcaagctta gccaacgttt tggtctgggc gatccccaca ccaacagtaa gatgggtacg
     9721 ttgtagcacc gttgcgcgaa tttctctgcc aaaatcagtc agatcgcgac aattacgcac
     9781 acctgtcaga tcgcagaatg cttcatcaat actgtaaatc tcgacgcggg gcgatagttc
     9841 ttccagcgtc gacatcaccc gattgctcat gtctgcgtaa agctcataat tgctgctaaa
     9901 gcaaaccaca ccacagcgac gaaacagatc tttttgtttg aaccagggat cgcccatttt
     9961 aacgcccagc gcctttgcct cagcgtttcg ggcgataacg caaccgtcat tattcgatag
    10021 cacaaccacc ggtttacccc ataaatcagg gcgaaacacc gtctcacagc tggcataaaa
    10081 cgcgtttaca tcacagaggg caaacatcag cgcatcgcct taacgacgtg gatcaccaca
    10141 ccaaagacat ccagcgtatc ttcgctactg atggtaatgg gcgagtacgc actgttcatg
    10201 ggaataagct gtaccgtcgg gcgtagttgc aattttttca ccgtaaactc gccgtcaaca
    10261 gcagcgatga caatatcacc atggctggcg gtaatagcgc tatcgacaat cagtaaatca
    10321 ccgtcactaa ttccaccatc aatcatagaa tcaccacttg ctttgacgaa gtaagtcgcg
    10381 ctgggatgct ggatcaacag ttgattcaga tcgatgcgct gttcaacgta atctgctgcc
    10441 ggtgaaggaa agccacactg aacaagatcg ctaaatagcg gaaaagtcac aatttcgcgg
    10501 agatccgcag gcttgataaa caacataata atctgcctga agttatactg tttttatata
    10561 cagtagtctg ttcttgccag cagatcaata ctgattcagg ctatcaatat ttgtcgctgc
    10621 atagactgct gatttttcgt tctcttatcc tgtgctcacg tggccttctg gcgacgacgc
    10681 tcatccagca gaaatgaaaa atatcacccg gcaaaaaaat agaatagaag cattcgccat
    10741 aatgacatta aacattgttt gatatttatc atattaatag aaataaagac attgacgcat
    10801 cccgcccggc taactatgaa ttagatgaag taaaatttat taatagttgt aaaacaggag
    10861 tttcattaca atttatatat ttaaagaggc gaatgattat gactgaaatc gttgcagata
    10921 aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga tggagcatta gatctttata
    10981 ataaatatct cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa
    11041 gtcgctttaa acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct
    11101 tacttatgga tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg
    11161 gtgttgcgac gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga
    11221 aagcatccgc ccctcattaa ggtactggat gacggcatca cgaagctgaa tgaagcgcaa
    11281 aaatccctgc tggtaagctc acaaagtttc aacaacgctt ccgggaaact gctggcgtta
    11341 gatagccagt taaccaatga tttttcagaa aaaagcagct atttccagtc acaggtagat
    11401 aaaatcagga aggaagcgta tgccggtgcc gcagccggtg tcgtcgccgg tccatttggt
    11461 ttaatcattt cctattctat tgctgcgggc gtagttgaag ggaaactgat tccagaattg
    11521 aagaacaagt taaaatctgt gcagagtttc tttaccaccc tgtctaacac ggttaaacaa
    11581 gc
//
LOCUS       DADLSE010000118        11541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLSE010000118 DADLSE010000000
VERSION     DADLSE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.118"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN692_003111"
     CDS             complement(520..1860)
                     /gene="fadL"
                     /locus_tag="JN692_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7265354.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            2232..2516
                     /locus_tag="JN692_003112"
     CDS             2232..2516
                     /locus_tag="JN692_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7265355.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2696..4006
                     /gene="fadI"
                     /locus_tag="JN692_003113"
     CDS             2696..4006
                     /gene="fadI"
                     /locus_tag="JN692_003113"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7265356.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVLLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            4006..6150
                     /gene="fadJ"
                     /locus_tag="JN692_003114"
     CDS             4006..6150
                     /gene="fadJ"
                     /locus_tag="JN692_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7265357.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPFIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6353..6838
                     /gene="sixA"
                     /locus_tag="JN692_003115"
     CDS             6353..6838
                     /gene="sixA"
                     /locus_tag="JN692_003115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7265358.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7521..8084
                     /locus_tag="JN692_003116"
     CDS             7521..8084
                     /locus_tag="JN692_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7265359.1"
                     /translation="MSKFVKTAIAVAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            8166..10807
                     /gene="yfcU"
                     /locus_tag="JN692_003117"
                     /pseudo
     CDS             8166..10807
                     /gene="yfcU"
                     /locus_tag="JN692_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            10827..>11541
                     /locus_tag="JN692_003118"
     CDS             10827..>11541
                     /locus_tag="JN692_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7265360.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGN"
BASE COUNT         2956 a         2675 c         2968 g         2942 t
ORIGIN      
        1 aagtagatgt ttaatacagg tgcttattcc ttccttacca ttaaaaatgg tttttgaact
       61 cttgtactaa tccattaatt tcatcattgt tattttcatt gatggattct gggtaagctg
      121 cgctggtatt gttgattgaa atagatggta gcatgatttt attccatgtt gattgatttg
      181 ttacaaatat atattaccta atgaaataga ctatcaaaaa acgctcattt atttagaacc
      241 tatctttaat ttgagtgtat aagcgcacaa tagccagggg ggttgagtat cctggaaatg
      301 cagaaaagta aactctttga gaaggacgag cagaacgctt gaaaatatgc atttcccttc
      361 aggtttgtct gtggatacca cttactgatt tgaaagggca agctgccatc aggctgtttg
      421 caaaactcag gaggcaagca atgaggaaac tactttcggt gaagtggggg agttaaagtc
      481 acctgcatag caggtgactt tatccaggcg aacgcgttat cagaacgcgt agttaaagtt
      541 agtaccgaac agccaggctt taccttcaga ctcgaactgg tatgggcctt cgttaatttt
      601 cacgctctga ccgtgcatat aagaaacacc aacgtcgact gaagcatctt tattaaatgc
      661 gtaagtcgta cctgcactca gccagaaacg gtcctggtcc ggaatggaga tagaacgatt
      721 ctgagccgga actgggctgt catcaaaggc gataccggta cggaaggtcc agttatcatc
      781 gtagtaataa gtggtaccca acgcgatgcg gtaagcatct ttaaagcctt catgtttctg
      841 gaacagcgtg tcgccactgg ttgaggtcgc tttcagctgc tggaactgac tccagctggt
      901 gtaagccagg ctatagtgaa tcgcccactg tggatcaaca cggttataac ctgacacttc
      961 ccacatttca ggcaggttca gcgtcagata acccgattgc gttgcgccac ctgtcgcggt
     1021 aggaattggc aaaccgtagt tattaaacgc acgattaaga tcgctgctgt agttaccttt
     1081 gaagtcaatt ttcacttcag aacggtaggt cagtgcatag cggttatttt tatccagttc
     1141 atacaggatt ccggcgttcc agccaaagcc ccactggtta ccgttcagat gagcgatttt
     1201 ggtattactg tcgataccgt tggcggtagc tgccaatgct tgcccttgag gagtttgccc
     1261 ggcaggagat tgcataatct gaccagcaac caactgcccc agatcgcctg cgaaacgttc
     1321 aattttcgcg cgagcgtaga tggcgttgaa accaagacca aagctccatg cattatttaa
     1381 gcgatacgca ccgcttaagt tcaggttcat ggtttcaagg tcagttgtac ccccgacaga
     1441 gccgcctgca taagtatcat taaactctgt tgccagacca tagttagagg taatagaagc
     1501 gccccaacca aattggtcgt taatcggtgc aacaaagtgc atgttcggaa cccatgccgt
     1561 aggcgcgatg ttatcggctt tcaggctacg accagacgga gacgttccgc tgatattcac
     1621 atccgggtca atataaaccg cacccgcaga aaatgtcggg cggtcaaaca tggtaatcaa
     1681 tgcggggtta cggctaacgt tacctgcatc atcggcaatt gcgccttccc ctgaataagc
     1741 ccggcccagg ccagaggaag aaaattcgtt taactgaaag cctgccgacc aggcctgggt
     1801 ggagataagt gccactgcga ctgcgagagc agactttgta aacagggttt tctggctcat
     1861 gaccataacc tcaatgattt atttttatac aaactatgtt acgtgctgta acaggagcgc
     1921 gaagtgtagg gtctgaggta catcttagaa atcagaccag tggcgagagt ataggtcgga
     1981 ccagctagaa tattgcaagt atgtttccat cttttttcaa atatgatcta agaaacgcga
     2041 tccagctggc aaaatttgga ggttgcgcac tcgcggattc gggtgaattt tgttttagat
     2101 catttttaag tgtgatttcg gtcacttatc cgattttgca gaattaacaa ctggagcagc
     2161 actttccgga agcgtaaaat atccacaacg tttatctggc accttgggtg cacatacaga
     2221 ggaaatcaac gatgagtaaa tgtagtgctg atgaaacccc ggtttgctgc tgtatggatg
     2281 ttggcaccat tatggacaac tccgactgca ccgcgtctta cagccgcgtg tttgcaaacc
     2341 gcgcagaagc agaacaaacg ctggcggcgc tgactgaaaa agcccgtagc gtggaatccg
     2401 aaccgtgcaa aatctccccg actttcactg aagagtctga cggtgtgcgc ttggatatcg
     2461 atttcacttt tgcctgcgaa gctgaaatgc tgatcttcca gctgggtctg cgttaattcc
     2521 tctcgggtac gtacgcccct gtcagttgct ggcaggggcg ttttcttttc tttcttctgt
     2581 cttcactgtg tcatagctcc cactttcctt tttctccgct tggctaaatg taaaaaaatg
     2641 gttaagacta tgatcaggtc agaccacttt atttattttt ttacagggga gtgttatggg
     2701 tcaggtttta ccactggtta cccgccaggg cgatcgtatc gccattgtta gcggtttacg
     2761 tacgcctttt gcccgccagg cgacggcttt tcatggcatt cccgcggttg atttagggaa
     2821 gatggtggta ggcgaactgc tggcacgcag cgagatcccc gctgaagtga ttgaacagct
     2881 ggtctttggt caggtcgtac aaatgcctga agcccccaac attgcgcgtg aaattgttct
     2941 cggtaccgga atgaatgtgc ataccgatgc ttacagcgtc agccgcgctt gcgctaccag
     3001 tttccaggcg gttgcaaacg tcgcagaaag cctgatggcg ggaactatcc gagcggggat
     3061 tgccggtggg gcagattcct cttctgtatt gccaattggc gtcagtaaaa aactggcgca
     3121 cgtgctggtt gatgtcaaca aagctcgaac catgagccag cgcctgaaac tcttctctcg
     3181 cctgcgtttg cgcgacttaa tgcccgtacc gcctgcggta gcagaatatt ctactggctt
     3241 gcggatgggc gacaccgccg aacaaatggc gaaaacctac ggcatcaccc gagaacagca
     3301 agatgcatta gcgcaccgtt cgcatcagcg tgccgctcag gcatggtcag acggaaaact
     3361 caaagaagag gtgatgactg cctttatccc tccttataaa caaccgcttg tcgaagacaa
     3421 caatattcgc ggtaattcct cgcttgccga ttacgcaaag ctgcgcccgg cgtttgatcg
     3481 caaacacgga acggtaacgg cggcaaacag tacgccgctg accgatggcg cggcagcggt
     3541 gatcctgatg actgaatccc gggcgaaaga attagggctg gtgctgctgg ggtatctgcg
     3601 cagctacgca tttactgcga ttgatgtctg gcaggacatg ttgctcggtc cagcctggtc
     3661 aacaccgctg gcgctggaac gtgccggttt gacgatgagc gaactgacat tgatcgatat
     3721 gcacgaagcc tttgcagctc agacactggc gaatattcag ttgctgggta gtgaacgttt
     3781 tgctcgtgaa gtactggggc gtgcacatgc cactggcgaa gtggacgata gcaaatttaa
     3841 cgtgcttggc ggttcgattg cttacggaca tcccttcgcg gcgaccggcg cgcggatgat
     3901 tacccagaca ttgcatgaac ttcgccgtcg cggcggtgga tttggtttag ttaccgcctg
     3961 tgctgccggt gggcttggcg cggcaatggt tctggaggcg gaataatgga aatggcatca
     4021 gcgtttactc ttaatgttcg tctggacaac attgctatca tcaccatcga cgtaccggat
     4081 gagaaaatga ataccctgaa ggcggaattt gcctcgcagg tgcgcgccat tattaagcaa
     4141 ctccgtgaaa acaaagagtt gcgcggcgtg gtgtttatct ccgcgaaacc ggacaatttt
     4201 atcgccggag cagacatcaa catgattggc aactgcaaaa cggcgcaaga agcggaagca
     4261 ctggcgcggc agggccaaca gttgatggcg gagattcatg ctttgcccat tccggttatc
     4321 gcggctattc atggcgcttg cctgggcggt gggctggagc tggcgctggc gtgccacggt
     4381 cgcgtttgta ctgacgatcc taaaacggtg ctcggtttgc ctgaagtaca acttggattg
     4441 ttacccggtt caggcggcac ccagcgttta ccgcgtctga taggcgtcag tacagcatta
     4501 gagatgattc tcaccggaaa acaacttcgg gcgaaacagg cattaaagct ggggctggtg
     4561 gatgacgttg ttccgcactc cattttgctg gaagccgctg ttgagctggc gaagaaggat
     4621 cgcccatctt cccgccctct acctgtacgc gagcgtattc tggcggggcc gttaggtcgt
     4681 gcgctgctgt tcaaaatggt cggcaagaaa acagaacaca aaactcaagg caattatccg
     4741 gcgacagaac gcatcttgga ggttgttgaa acgggattag cgcagggcac cagcagcggc
     4801 tatgacgctg aagctcgcgc ctttggcgaa ctggcgatga cgccgcaatc gcaagctttg
     4861 cgtagtatct tttttgccag tacggacgtg aagaaagatc ccggtagtga tgcaccgcct
     4921 gcgccattaa acagcgtggg aattttaggt ggtggcttga tgggcggcgg tattgcttat
     4981 gtcactgctt gtaaagcggg gcttccggtc agaattaaag atatcaaccc gcagggcata
     5041 aatcatgcgc tgaagtacag ttgggatcag ctggagggca aagttcgccg tcgtcatctc
     5101 aaagccagcg aacgtgacaa acagctggca ttaatctccg gaacgacgga ctatcgcggc
     5161 tttgcccatc gcgatctgat tattgaagcg gtgtttgaaa atctcgaatt aaaacaacag
     5221 atggtggcgg aagtggagca aaattgcgcc gctcatacca tttttgcttc gaacacgtca
     5281 tctttaccga ttggtgatat cgccgctcac gccgcgcgac ctgagcaagt tattggcctg
     5341 catttcttca gtccagtgga aaaaatgccg ctggtagaga ttattcctca tgcgggtaca
     5401 tcggcgcaaa ccatcgccac tacggtaaaa ctggcgaaaa aacagggcaa aacgcccatt
     5461 gtcgtgcgtg acaaagccgg gttttacgtc aatcgcatct tagcgcctta cattaatgaa
     5521 gctatccgca tgctgaccga aggtgaacgg gtagagcaca ttgatgccgc gctagtgaaa
     5581 tttggttttc cggtaggccc aatccaactt ttggatgagg taggaatcga caccgggact
     5641 aaaattattc ctgtactgga agccgcttat ggagaacgtt ttagcgcgcc tgcaaatgtt
     5701 gtttcttcaa ttttgaacga cgatcgcaaa ggcagaaaaa atggtcgggg tttctatctt
     5761 tatggtcaga aagggcgtaa aagcaaaaaa caggtcgatc ctgccattta cccgttcatt
     5821 ggcgcacaag ggcaggggcg actctccgca ccgcaggttg ctgaacggtg tgtgatgttg
     5881 atgctgaatg aagcagtacg ttgtgttgat gagcaggtta tccgtagcgt gcgtgacggg
     5941 gatattggtg cggtatttgg cattggtttt ccgccatttc tcggtggacc gttccgctat
     6001 atcgattctc tcggcgcggg cgaagtggtt gcaataatgc aacgactggc cacgcagtat
     6061 ggttcccgtt ttaccccttg cgagcgtttg gtcgagatgg gcgcgcgtgg ggaaagtttt
     6121 tggaaaacaa ctgcaactga cctgcaataa gaaggtcaaa gctatatgaa tccgcgctga
     6181 atggcggagt gttggtcaaa atgtaaacgc atattgacta tacttacgcc attgaggtaa
     6241 aaaacagcgt ttcattcggt gaatggataa ggcacaatgc cggccaccgt tttctttctc
     6301 tggtttcaga tgaaagaaaa cgggcgaatc tggttaacaa aagcggtgca atatgcaagt
     6361 ttttatcatg cgtcacggcg acgcagccct cgatgccgcc agtgattcgg ttcgtcctct
     6421 gaccactaat ggttgtgacg aatctcgcct gatggcgaac tggctgaaag gtcaaaaagt
     6481 ggaaatcgaa cgcgttctgg tgagcccgtt cctgcgagcc gagcaaacac tggaagaagt
     6541 aggggattgt ctgaacctgc cttccagtgc agaagttctg ccggagttaa cgccctgcgg
     6601 cgatgttggt ctggtcagtg cctatttgca ggcgttgacc aatgaaggtg ttgcttcggt
     6661 actggtgatc tctcacttac cgttagtcgg gtatctggtt gccgagttat gtccgggcga
     6721 aacgccgcct atgtttacca cctcggccat tgccagcgta acgcttgatg aaagcggaaa
     6781 cggtacattc aactggcaga tgagcccgtg taatctgaag atggcaaaag ctatctgatg
     6841 tgttccaaat tatgcccaat gaggtagatt cattgggcat aattgctcat taggcagcgg
     6901 atatctgtca acctcactac tattgaagac tctccttatc tataaataaa acactttcta
     6961 cattcattcg gaaattattc ttctgcgcgt atcttgattt tgcccaaagt tacatgttga
     7021 aatgaataat gtctgtttga cactcattgt catttattaa tacagataat agagaaaata
     7081 gagttgaata atcaggcagg atgtaattga aacttgatta aattctggct atagtgttta
     7141 tatattatat tgtcaaaagt ggataaatgc caatcgcatt atatgttcat tgtttgtttt
     7201 ttaagctgtt gatattcatt attgatatac tttctatttt ttctaaatac ttattccgta
     7261 ttttttaagg atgtccttaa gataaccttt cttctgtcaa tatattaatt aaatctacat
     7321 ttactcttta gtaaaattaa attggctcag gggtgtggaa acatcactat gcggcccaca
     7381 ccaacgaata caattttcta cgacctgttc tttttgggtc gcatgccgta ttcaatggct
     7441 gggtataaac attggctatt gactgaatca atgtattgca atgtttatat ttttaattat
     7501 ttatagttta aggacaaata atgagtaagt ttgttaaaac agctattgct gtggcaatgg
     7561 tgatgggcgt gttcacctct acggcaacaa tcgctgcggg taacaacggt actgcacgtt
     7621 tttacggcac cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac aaactggaag
     7681 ttgatatggg tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc acgccgaaaa
     7741 gtttccagat tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg accactacct
     7801 ttactggtac cgtttcttct gcaaatagcg gcaattatta caccattttc aataccgata
     7861 ccggtgcggc atttaacaat gttagcctgg cgatcggtga ctctctgggt acctcttaca
     7921 aaagcggcat gggtattgac cagaaaatag tgaaagacac ttctaccaac aaaggcaaag
     7981 cgaagcagac actgaacttt aaagcctggc tggtcggcgc agctgatgcg ccagatctgg
     8041 gtaattttga agcaaatacc accttccaga ttacttacct gtaaggcata cccccatagc
     8101 gacactgcta tgggggattt aaatggattt tgaccgaaca agaataaaaa cggatagaaa
     8161 cgtgtatgcc tgaccattct ctttttcgat tacggattct tccgtggtgc attgtgctgg
     8221 caatgtcagg gagttatagc agtgtctggg ctgaagacga cattcagttt gattcccgtt
     8281 ttctggaatt aaaaggcgac acaaaaattg atctgaagcg tttttccagc cagggatatg
     8341 ttgagcccgg aaaatacaat ttacaggttc aactaaataa acagccattg gcggaagagt
     8401 acgatattta ctggtatgct ggtgaagatg acgcgagcaa aagctatgct tgtctgacac
     8461 cggaactggt agcgcagttt ggtttaaaag aagacgtggc gaaaaatctg caatggagcc
     8521 acgatgctaa atgcctgaaa tccggtcaac tggaaggcat ggaaattaag gctgatttaa
     8581 gccagtccgc attagtcatt tcactgccac aggcttacct cgaatatact tatcccgact
     8641 gggatccgcc ttcacgttgg gatgacggca tctccgggat cgtcgcggac tacagcatca
     8701 acgcacaaac ccggcacgaa gaaaatggcg gtgatgatag taacgagatc agcggcaacg
     8761 ggacggtcgg ggttaacctg gggccgtggc gtatgcgtgc tgactggcag actaactatc
     8821 aacatactcg cagtaatgat gacgatgaat tcagcggcga tgaaactcaa aaaaaatggg
     8881 agtggagtcg ctactatgcc aggcgggcgt taccatcatt aaaagccaaa ctggcgctgg
     8941 gcgaggatta cctcagatcc gatatttttg atggttttaa ctatgttggt ggcagtgtca
     9001 gtactgacga tcaaatgttg cctcccaatc tgcgcggcta cgcgccagac atttccggcg
     9061 tggcacacac cacagcaaaa gtgaccgtca gccagatggg gcgtgtgatt tacgaaacgc
     9121 aggttccggc tggaccgttt cgtattcagg atcttggtga ttctgtctcc ggtacgttgc
     9181 atattcgcat tgaagaacag aacggccagg tgcaggaata tgacatcagc accgcctcga
     9241 tgccatacct cactcgtcca ggtcaggttc gctataagat catgatgggc cgtccgcaag
     9301 agtggggaca ccatgtcgag ggtgaatttt tttctggtgc tgaagcttcc tgggggatcg
     9361 ctaacggctg gtcgttatat ggcggcgcac tgggagatga aaactatcag tctgcggcgc
     9421 ttggcgtcgg tcgcgatttg tctacattcg gcgcggtcgc gtttgatgtt actcactcgc
     9481 acaccaaact ggataaagac accgcttatg gcaaaggttc gctggacggt aactccttcc
     9541 gtgtgagtta ttccaaagac tttgaccagc tcaacagccg cgttactttt gctggatatc
     9601 gcttctcgga agagaacttt atgaccatga gcgagtatct ggatgccagt gacagcggaa
     9661 tggtacgcac gggcaacgac aaagagatgt acaccgccac ttataaccag aacttccgcg
     9721 atgcgggtgt ttcggtttat ctcaactata cccgccatac ctactgggat cgcgaggagc
     9781 agacaaacta caacatcatg ctctcgcact atttcaatat gggcagtatt cgtaatgtca
     9841 gcatctcgat gactggctac cgttacgagt atgacaacca ggccgacaaa ggcatgtaca
     9901 tttcgctcag tatgccgtgg ggcgacaaca gtaccgttag ctataacggt actatggcag
     9961 tgggacggac agcagtcagg tcggttattt cagccgtgtc gatgacgcga ctcactatca
    10021 gttgaacgtc ggcaccagtg acaaacacac cagcgttgac ggctattaca gccatgatgg
    10081 ttcgctggcg caggttgacc tcagtgcgaa ctaccatgaa gggcaataca cctctgcggg
    10141 cttgtcgtta cagggcggcg caacgcttac tgcccacggt ggcgcactcc accgtaccca
    10201 gaatatgggc gggacacgct tgttgattga tgccgatggc gttgccgatg ttccggtaga
    10261 aggtaacggt gctgctgttt ataccaatat gtttggtaaa gccgtcgttt ctgacgtcaa
    10321 taactattac cgcaatcagg cgtatatcga cctcaacaaa ctgccggaaa acgccgaagc
    10381 aacccagtcg gtggtacaag ccacgctaac tgaaggggcg attggctacc gcaaatttgc
    10441 cgtcatcagt ggtcaaaaag cgatggcggt gctgcgttta caagacggca gccatcctcc
    10501 gtttggcgca gaagtaaaaa atgataacga gcagacagtg ggccttgtcg atgatgacgg
    10561 caatgtttat ctggctgggg tgaaacctgg cgaacacatg agtgtgttct ggagtggtgt
    10621 tgcgcattgc gatatcaacc tgccggaccc gctgcctgcc gatctgttta acggcttgtt
    10681 actgccatgc cagcataaag gcaacgtcgc gccggttatt cctgatgaca taaagcctgt
    10741 cattcaggag cagacgcaac aggtgacacc caccaatccc cctgtttctg tttcagctaa
    10801 ccaataacgt gattaaggaa tgatccatgt ctgatttact ttgctctgcc aaattgggtg
    10861 ctacgacgct agcgttactg cttagcgctg ccagtttgtc tgcccaggct tctgtaacgc
    10921 cagatcgtac ccgtttgata tttaatgaaa gcgacaaatc aatcagcgtg acgttgcgca
    10981 ataacgatcc gaaactgcct tatctggccc aaagctggat cgaagatgaa aagggtaaca
    11041 agatcagttc accgttaact gttctgccac cggtgcaacg catcgattca atgatgaacg
    11101 ggcaggtaaa agtgcaggga atgccggata tcaacaaatt gcctgccgat cgtgaaagcc
    11161 tgttctactt caacgtgcgt gagatcccgc caaaatcaaa caaagccaac acgctgcaaa
    11221 tagcactaca aacgcgtatt aagttgttct ggcgaccgaa ggcgctggag aacgtcagca
    11281 tgaaaaaccc ctggcaatat aaggttactt taacccgcaa cggtcaggaa tttaccgtca
    11341 ataacccgac gtcgtattac gtcattttca gtaatgccag cacacagaaa aatggcaatc
    11401 cggcagccgg tttctcgcca atagtgatgt cgccgaaaac ctctgcaccg ctgaatgtga
    11461 agatgggtag cgtgccggta ctgacctatg tcaacgacta tggcgctcgg atgccgctct
    11521 ttttcacttg caacggtaac a
//
LOCUS       DADLSE010000119        11490 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLSE010000119 DADLSE010000000
VERSION     DADLSE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.119"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..710)
                     /locus_tag="JN692_003119"
     CDS             complement(<1..710)
                     /locus_tag="JN692_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7265361.1"
                     /translation="MSGIFVGLNSHIMEGFDSVLTSVAADTGGWMAHLFSIAVLSLYI
                     TWRAYQTMAGKLVRPFEEIVWDVSRMLIIMIFVTNAGGYLDLVSDAITGLRDGFSGDA
                     SIWARLDTVWEKAQALGEKLYSYDDSTYVALKGTLAQFLVWAGVMLLVGITAIINLIA
                     ELVMLLMLATAPIFIYCLMWSWFRDMFSNWLRIILGCILTSLFSGIALTVVMDYLDYI
                     LAGATKASDANYLTLAVQV"
     gene            complement(728..1012)
                     /locus_tag="JN692_003120"
     CDS             complement(728..1012)
                     /locus_tag="JN692_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7265362.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(1024..1734)
                     /locus_tag="JN692_003121"
     CDS             complement(1024..1734)
                     /locus_tag="JN692_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7265363.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            complement(1759..4509)
                     /locus_tag="JN692_003122"
     CDS             complement(1759..4509)
                     /locus_tag="JN692_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7265364.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(4519..4818)
                     /locus_tag="JN692_003123"
     CDS             complement(4519..4818)
                     /locus_tag="JN692_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7265365.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(4829..5491)
                     /locus_tag="JN692_003124"
     CDS             complement(4829..5491)
                     /locus_tag="JN692_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7265366.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(5494..5709)
                     /locus_tag="JN692_003125"
     CDS             complement(5494..5709)
                     /locus_tag="JN692_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265367.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(5702..6010)
                     /locus_tag="JN692_003126"
     CDS             complement(5702..6010)
                     /locus_tag="JN692_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265368.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            6324..7238
                     /locus_tag="JN692_003127"
     CDS             6324..7238
                     /locus_tag="JN692_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7265369.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            7242..8225
                     /locus_tag="JN692_003128"
     CDS             7242..8225
                     /locus_tag="JN692_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7265370.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            8218..8706
                     /locus_tag="JN692_003129"
     CDS             8218..8706
                     /locus_tag="JN692_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7265371.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            8703..10421
                     /locus_tag="JN692_003130"
     CDS             8703..10421
                     /locus_tag="JN692_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7265372.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            10685..10894
                     /locus_tag="JN692_003131"
     CDS             10685..10894
                     /locus_tag="JN692_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265373.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            11103..11237
                     /locus_tag="JN692_003132"
     CDS             11103..11237
                     /locus_tag="JN692_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265374.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(11280..11490)
                     /locus_tag="JN692_003133"
                     /pseudo
     CDS             complement(11280..11490)
                     /locus_tag="JN692_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2995 a         2639 c         2930 g         2926 t
ORIGIN      
        1 acctgaacgg ccagagtcag gtagttggca tcggatgctt ttgtggcgcc cgccaggatg
       61 tagtccaggt aatccattac caccgtcagg gcgatgccgc tgaacaggga tgtaaggatg
      121 cagcccagaa taattctgag ccagttgctg aacatatccc ggaaccacga ccacatcagg
      181 cagtagataa agatcggggc ggtcgccagc atcagcagca tcaccagttc ggctatcagg
      241 ttaatgatgg ccgtgatgcc taccagtaac atgacgcctg cccagaccag aaactgcgcc
      301 agagtgcctt ttagagcgac ataggttgaa tcatcataag agtaaagctt ctccccaagt
      361 gcttgagcct tttcccagac ggtatccagc cgcgcccaga tcgacgcgtc gccgctgaat
      421 ccgtcccgca ggccggtgat ggcatccgaa accagatcga gatagcctcc ggcgttggtg
      481 acgaaaatca tgatgatgag catccggctc acgtcccaca cgatctcctc gaaggggcgg
      541 actagcttgc ctgccatcgt ctggtaggcg cgccacgtga tgtacagcga gagcaccgcg
      601 atggagaaca ggtgtgccat ccaccccccc gtatcggcgg ccacgctggt gagcacgctg
      661 tcaaacccct ccatgatgtg gctgtttaat ccgacgaata ttccactcat ggtgcgttct
      721 cctgtcgcta attcaggtca gcaataggct cttgcacgcg agctttatca aggtctttca
      781 ggatttgctt atatccttcg gcaacatcag gtttagcttt ttcacggtga agggcggagc
      841 gtgcattctg gcaatcggtg ggcacaaaat tatgattaaa aatgcactcc ctgtatttcg
      901 ccagcatgtc gtcatggtgt gccatatacc agtctacgga ggttttatcc tcgcagccag
      961 taagcgcggc cactgaggcc agaacaataa aggtcagtgt cttcagtttc atggggtcac
     1021 ctgttagtta aggtctgcga ttggagctgt gcgcatttgc tcggcctgcg cctttcggcg
     1081 ttgttcggca agcagctggt tgcgcatctc actttgtttg accgacattt cccattgagt
     1141 ggtcagggcg ttcagctgga tactccgcgc ggacactacg ttcgccagat cctgcgattc
     1201 tttactgtcc ttcgcattct cgatgcgact ggcgagatta ctaatatcgc tcagcgtgtc
     1261 ggaaattttt tcctggatgt cagaagtgtc ctcgacggac accgcctgat tgaggagcat
     1321 ctgtttgcag ctatcccggt acgcctcgct ggcgttctcc gggcagttgt cgaacagttt
     1381 gtatttgtca tacagcctgt ttagttcccc agcataagac ccgcctgagc cggttaacag
     1441 atcgtttaag gaaatccccc tgtttttcag atttttaatg tcagccgtca ggttttttgc
     1501 ctgattcagg aatgcctgga cgtcccgaat accggtggcc gttgccagct gttgtttata
     1561 agcgttcatc tgcgactgat aatgttgaac cgtctgctgc cactgctgga gcttctccgc
     1621 ccactgctgg atgttgctgg cgttggctac ttcatcaaaa acgggaatac cggcctgagc
     1681 cgggacgtga aagagacaca aggcgacggt cagcgccgtc agggtggttt tcagtttcat
     1741 ggtgttatcc ttttgtgatt aaacggcacg tctcagcatt tcgtctttcc agtcttccgg
     1801 cttcataccc tcctgccaga tctcgtcaaa aattttcagg ttgtccgcac tggcgctgag
     1861 cagcttcgtg tagcgaccta gcccggagag gtcgagggtg atttgtgcga tatacgggcg
     1921 cacgtcacca cgccgcagcg gagatttcat gatcaccatc tggcgggcgt aggggtcgag
     1981 ggtggtcacg atgtcgtaaa ccgctttcgg cacccgcagc atgtccacgt agtcgtactc
     2041 cttcgcctgc tggttcgcca tgaagatgcg ggtactacac tgctcgataa tggccgccga
     2101 gatctcgtgc ttgcacatct cctccggcga ctgcgtggcc gggaagaaaa tgccgttgag
     2161 ctttcgaatg accttcagca tgtcgagggc gaattttgaa aactccgggt cctgaagcca
     2221 tttccagaat tcatccatga aaatgaccag gcggcggcca tcgaggaggc tggtcacgcg
     2281 gtagagcagg tagaaggtga gcgggctgcg cacgtctttg tcgttcagga actccgtgcc
     2341 gtcgatgccg aaattatcaa tattgtcgat atcaaaggtg tccacgtcat tatcaaacac
     2401 ccagccaaac tccccgccct gcgcccactg ctggagacgg atgcgcagcc cgttcatccg
     2461 tgcgtccttc gtcggggctt catgcagcac ttccagcatg cgggtgatgc cgttgcgacg
     2521 aagctcaggc gggtagtcca gcatgatggt gtccactgcc tcgctgaggc gttcttcatc
     2581 gcgcgggtcg agcggtttgc cgtcccttgt gcacatcatt cgcatcagct tcttgaggaa
     2641 gttgcggtta cgtttcgttg gagcgagcgc aaacgggtta aggccggtct tctcgcctga
     2701 acggaaacgg aagtacgagc cgccctggag gcgtatcgac atttccgccg aacggtcttt
     2761 atcaaagacc accgaggtga agcgcttttt cgtcgccccc ggcgcaaacg actcaggacg
     2821 ccggtatttc tgcatcagat gctggaacat catcagcagg gtcgatttcc ccgtcccggt
     2881 actgccgagc agcatggtgt tacccggcgg cttctcgctg aactcgtcaa catcggcctg
     2941 gctgttgtgc agattcacgt actgacatcc cccggacggg gtcggaacaa cggcaatcgc
     3001 ctccccccag gcattcccgt cccgcttgtg cgggtgaaag ttgtgaaacg acaccatttc
     3061 ggcgtagttg aggctgctca ccggcaccag acgcgggcgc aggtgataca cacccggcaa
     3121 ctgcgcgaga tacgccgccg acagcgactc gcgggacagg gtcatgatga aaccgagatc
     3181 cttcagggta tccgccaggg tgttggtgtc ccggataacc tgctcgctgg ttggggcgct
     3241 gacaataagc gtctggtgat atttcccgaa tgaaataatg ccggactgaa gcatgtcgcg
     3301 ggcgaccttc aggtcttcct gctgggagat cgcatcatcc tcggcagact tcaggcgttt
     3361 ctcgatggtg cggatgcagc cccgcgcttc atccttaccc atgcaggtat aggactgcgt
     3421 gaacacgtac cggcagcggg cataaagaag cgagtcatac agcccggtga tggtttcagt
     3481 gctgtaatcc ttgatttcca gcccgcgaaa gtagctctgg ccgtcggtgt ggctgacctg
     3541 gccaatgtcg gtggaaggga aaatatccgt gccgcccagc acgtcataaa acggcgtgcg
     3601 ggtgacgcgg actttctgcc acctgccggt gatcagaaag ttgtaaaacg ccagctggga
     3661 ggagaacacg tgcccgttct cctcataagt gccaagcggc cgcgccgcgt agcgtgacag
     3721 cgccgtgcca atcgcttgcc ggtgctcctg catcacctgc agggcattgt cgagcagggt
     3781 tttacgttcg cccagggact tctgtttcat ggtcgcccgc tcaatgccgg tgtgcggatt
     3841 gaagcagacg gtgaagaaga ggcgacgctg gtagaaaggt ttgccctcgc gggaggcgta
     3901 aaacagacgc gcgatttcat ctgaaaacgg aatcccggat tcacattcaa gcgtgtcggt
     3961 aaattcgtcg ctcaccagat gggtatagaa ggtaacgggt agcccctcaa aggagcggat
     4021 aacgttgttg agctgcatgt ccagggtggc gaggtaatgt tcatcctcgc attcaaaaat
     4081 gataccgccg atctcccagg tgctgacaaa gtcacctttt ttgttcttca ccacctgctc
     4141 gtgcaggtgg gaagaccagg ggagatagcg ggacagcggg atactctcgt tgagccgcat
     4201 atgttttata aactccgtaa cgtctatggc gtcgtactgg tttgcgacaa gcgcctttgt
     4261 cccgaaatgc cgggtcgccg cgcggttgcc acgcgtttta aacgccagca acttcaggtc
     4321 aaagaaatga atgtcctctt ttgccagccg tctcagctcg ataaaggcag gtatgagaag
     4381 caggagaagc cagtaaaaac gtgaccagac ggccgccagg gtaaggatgc acagggtgaa
     4441 cagcaggggg acaagcggga ccccccacag cgccgccggt cgggtcagcg ccttgttgag
     4501 gatggtcatg ctgcctcctt aggcggcgaa gtaggcaccg aacccggacg ccccggcgag
     4561 caggacaccc gcccagaaca cgcgctggag atcgtgccag gccttgccgt caacaagcat
     4621 cttgtaggcg ataaaggcca ccgcacagct gatagtgacg actgacatac cagttaggcc
     4681 gaggaagacc cagttaatgg cgtcactgcc tttctgaaat gcctggtcag cgctggccgc
     4741 cattgctgga acagcagaca gggcggaaaa tgcggcaatc aggcgggttg cagcggtttt
     4801 gatagtacgt aaagtcatga tctcttcctt aatcagaaga cacagcgagg ccgccgcgca
     4861 cgacatgtgg cggataagca gcgatgctgg tcggggtggt gtcgggtgcc ggaattacgc
     4921 cgcgtatgac gctatccggg taaacagtgt tgtcggacgg gacggcaggc gcttccccgg
     4981 atgagggaga tgctgtctgc tgttctgccc gcgttgaggg cacaacgtag ccaatacgct
     5041 gtacgtagct tgtgttgccg aacgcgcttt ccgggcgctg gccggtttca aagttgcctg
     5101 agtaatagca gctcagggcg cgctgtaatg ttcctcctcg ttgatagcag tcggtgatga
     5161 ttttcgcggc aacagtcatg ttgtcacagg ggttaaacat ggattcggcg cttacgccga
     5221 actgcgggaa gttggtgctg gttatctgca tcagccctac tgaataacgg tgctttttct
     5281 gctttatggc ctccacaatt tttagcgcct cgtctttcga cgaaggcaga tgagaaatca
     5341 cgcggctcct gccgcccttt accgggacaa tctcagcgat ggcatatgga tttaagccgg
     5401 attcggtgcg ggtgatgtcg tggatggtat cggggtgaac ggcgggagca cactgcattg
     5461 cgagctgaaa caacgcggca gtggttaaca tagtcagtcc tttctgtaga gcagtacctt
     5521 accggtggca cagtccgggt tgataaataa ctgaatgtcg ccctggctgt tagcttgggc
     5581 aacaccgatt tttgtaagcg tcattgccag ccgatcaccc ttcaccgaat acacgttgtg
     5641 cgtgtactcg gtcatggggg tgaatacggc ttcaagcagt gaggccggag caggcgtgat
     5701 gtcaggcatt acatttccta ttttccagca gtgaggtgat tgcatcgata cgtcgcagcc
     5761 tgtcggcaat aagtacggat acatcggatt ttttgagtcc ctgcgctttc acagtcaggg
     5821 tagtgatttc tgcccgcagg gctgcccgtt caattagcaa ttctcggacg ttatccggca
     5881 gctccctgag cgaccaggtg ccattaatgg cacgcgtgat acggtcacgg gaggagcgta
     5941 gcggcggctt gccgagagtt gaataaggat tctgacgcag cgccttacgg cggacggttt
     6001 gcatgctcat ggctgtttct cctgggtatg agtaagccac ccgcgtgcgg tgcacagtgg
     6061 gagacaggga aggattaaac gggaggctta acgatacaga ggagtgagag tgttacccat
     6121 tacaggcgcg gcacgtcatc tctttacgca gtcacagaaa acacctcctt atcctgaccg
     6181 gggaacgccg ctgctaggtt attaaagagc taatgatttg attaatacaa tagtgcttat
     6241 aagttgggta ttcaataaaa ttataactta tagtgtcgat ttaagaatga ttccaatttt
     6301 taggactttt tcagctgtgt aaaatgttgg agatgtttga aaaaattgaa aaaaaatact
     6361 ggcaggataa aatcaagaaa atctccaaat tcagagtaat aaaaatgcaa gatctcgatg
     6421 atgcaaaagt gatagaggta ctggcgaagg tattgaccaa aaaaggtatt tccaaggtaa
     6481 agcacaatag ttacataagt aaggtgctac agatcactac atcagcgggc tataagaagt
     6541 tgaatggtgc gaccaagtgg gaggttcagc aactcataaa agtagtccaa tccgttggca
     6601 tggatatgat tgagtttttc cgtatttatt atgaggataa taaagaaatt caagatgcga
     6661 tctggaacga tggccgcagt gaacactctt gtaaggtatg cctctatcct gaaggttcgg
     6721 aagtaaacac agaatactca gcactgaaag tagctgacca atggaacatt tttccgacaa
     6781 atgatttaaa agatgaactg ctttatgaat caaaacgaag catagaaagt attgtcataa
     6841 atccaaaggt aattagcagc actaagtatc gaattgcact acttgatgac gatgctaata
     6901 tcaccgaaag cttaaaagaa atcctttcga acgattatta tcaagtagat acttatgata
     6961 acttgaaaaa acttactcaa gctgtcgcaa caaatccgta tgacgcatat gttttggact
     7021 ggatcatcgg tgatgaaaca agctttaagt tggttaaagc tataaggtcg tcaaaaaata
     7081 aaaatgcaat gataatcgtt cttacaggtc aactatctgg tataaaagat gatgaaattg
     7141 taaagtcaat tcatgattat gacattgtgg gaccatacga aaagccgatt aaatcaggca
     7201 tcatcaaatc taacattgaa aaatatttcg ccagatagat tatgagaaat ttacttgaat
     7261 attccggtag cttgataagt aacgatagtt tgatcgtagc accttacgat tgtcagaagc
     7321 taagctatga aaatatttta ccattaaaca atggtcttat aaaaaactgg aatatacaaa
     7381 aattctcaag tgattactct gaaatagaac acctaaatgt gatcaatggc atgggagtga
     7441 cattaggtga ttctatcgtc gggatttctg tgctgtcagc aatcaaaaaa aagaatccca
     7501 agattaaaat taagcttatt cgaccggaga ctgcaccgtc atatgttgag gaactgtatg
     7561 aattagcatc ctcggttata gatgagcttc attacatgcc ttttcatatt cagaaattgc
     7621 cttgtactgc attgaatatt gatgtgggaa atcagctata ttctccagat ttccatatta
     7681 tggaaatgca tgattatttt atccaaggtc ttggcgtgaa acttgatgat gttaaccata
     7741 atgagctatc taataaatgg ttaggcaata ttgatctcgg agcgccagtt tttgaaaact
     7801 acacactttt tgcacctatt gctagcacaa aaataagaag tataccgtcg aagttctatt
     7861 atgacatagt tgatatgctt tctaggcgcg aaggaaaaac ggtactaggt ttcgtggatg
     7921 tgaatcataa aaattatatt aacatatcaa agtattcacc ccatacgaaa gatttcatag
     7981 cgctaattaa gtatgctagc aacgtttata cttgtgactc ctctgcattg catatagccg
     8041 ctggatttgg tgtacctact gaatgcgtgt ttaatacaat accgccagaa ttaagaacaa
     8101 agtattacag taagtgtaag tcgatttatg ttgggaataa aagattagaa aaaattcaga
     8161 gtagtgaaga tttccaactg gttaaaatgg tggaaaataa ttacagggag tatctttatg
     8221 gttaaaataa tatcattctt atccctaatg cttttgtcaa ttagtgcata tagcaatatt
     8281 ggggatatag ttatagatgg ttcaatcgct aatgctaaca gtgataaagg ttatgtgatt
     8341 actgaaaagc agtttgattc gttcccacgg gataccatcg taacgacaac accttggacg
     8401 ccaaccggta aaaaagtaaa attcaagggc gtcgacctaa gttatgtttt aaaactcgct
     8461 ggcgctaatg gagcaaagct aaaattccat gctctgaatg attatgaaat cattgtagac
     8521 atgaatgacg ttgaaaaata taacatttta ttggctacag aaatggatgg aaaaaggctt
     8581 caaataaggg attttggacc ttattttttg gtatatcctt tagatgaaca ttatattgag
     8641 cttaacacgc cacactatct tgcacggttc atttggcaga tagacaaaat tacggtgctc
     8701 aaatgaaaaa aaatttcttt atcattttgg ttgctctgct tttgttgata gcattcactg
     8761 tgtctagtgt tgttaagtac accagtcttt tatcggagaa aaatttatac accgtcgctg
     8821 gcacccaaga aaactacggt ttctcagtgg caaaattcat tatacagttg aatgagcttt
     8881 acacgctaat caatagcgat aatactattg acgatgttag gcttaaattc gatatccttt
     8941 attctcgttt gaatgttatt tatgtaaaga gtgaggcgac tgcacctcta tataaacagc
     9001 aaggctatga agaaacaatt gattcgataa acaaaaagct tgaagacata gacgggctgc
     9061 tttcgcataa tcaccctgat tataaaaaaa taagtacaat catagctgat ataaaaccgc
     9121 taactaaaac cgttacaaat cttgctgata tggctgaaat agcacaaagg aatgacgctc
     9181 ttaaagattt caggagcaaa cgtcagcagt tatggactct gctttttatt acaggtgggt
     9241 tgatttcctt acttcttttt gtcctattta tttacataag taaaattaac cgattgttac
     9301 tatcagaacg ggctgctttt gccagtaaaa atgcattttt aggtatggta ggccatgaac
     9361 tcagaacatc tttacaggca atagtgtcta tcattgatgt cgtaaccaat aatctgagtg
     9421 gagggattaa atctggtcag atagaacgac ttgaaactgc tgtttcgaaa atggagcgcc
     9481 agttaaatga tttggctgaa tttgcaaaaa ttgacaacgg atctgtagag ataaaaaaca
     9541 cctataatag cttgcaagca attgttacta acgctgtgca ggattgcatt gctatttatg
     9601 aaaaaaagga tgtcacagtt aaaataaaaa acaataatga tgcagtgatt tttacagatg
     9661 ctctacgcct taatcaagta attgaaaact taaccagtaa cgctattaag tacactgagc
     9721 gcggggaagt taatgtagat tactttatag aaaaaggtaa ggtattaaat attgtcattt
     9781 ctgataccgg aaagggaatc cctaaagaca aactaaagtt catatttaaa ccatttacac
     9841 gagttgtaga ttcgaaatcc actgtacctg gatttgggat gggacttgct attgttgcag
     9901 ggataatcag actattaaaa ggtactattc atatatctag tgaagtaaat gtaggtacaa
     9961 ctgttacagt gagaatacct atcaagttgg gagatagcag ccttgcaaca gctgaggtat
    10021 ctgcttcagc tcccggagat gtcttcggcg ttcttagctt gcttgttatt gatgataatg
    10081 aaatggcctg ttcgtctctt tccagtctgc ttactaatgc tggatacatt gtcgaggcta
    10141 caacctcgcc tgaacgagcc ctggaaaagc ttctgcgtaa accttatgac gttgtcctga
    10201 gtgatttaca aatgccaggc atgacaggtg atgagctgta tgcaactgtt agtagcagtc
    10261 agaatcctaa tcgatccacg cctttcatat tcatcagcgc atacagtgat gcacactcta
    10321 tcggacaggc tcctttgtta actaaacctg tcaggttaaa agatataaac cttaaaatac
    10381 atgaggttat gtcaaatcaa ggacgagtca aaagtgagta ggcattgtcg taatatttgc
    10441 gattatagct ttggtgttct cgatcgttgc gaattttgaa aatttgtgga gtcaaatggc
    10501 gccgaaagcc cggcgtagcc ggttacctgg ctataaactc tgccacccta ccccctgccc
    10561 tgattagcct taacccgctt acagcaatga agaagtaaaa gtaatccctt ttaaccccgg
    10621 tgagcgggct ttacggcgtt tacgacgtaa agggcgtgat ttccggggcc ttagcggcgg
    10681 cagcttgctg ctgacgctta gggggatgtt cttcagttat ttcagcgaca ccagggaact
    10741 taacacggta gttgcaaagc tatacccgct aaaaatgtct cagatgcctc tgtacgcggt
    10801 cggagtggtc ccggcaaggc gtcagccgca gccggggcgt atctccgcgt tagcgggccg
    10861 aagggccgct tacgagcgtg tacccgacaa ctgaccgcag ctccacccac agcgatgaag
    10921 aagagtagtt acaacaccca tcattaaaag cgacactact cccggccttc ggccgggggc
    10981 ggcggcgctt ttcagtggtg gggaggggca cccggccggc aaaccggcgc cggttttatg
    11041 atgagctttc ttttgaatgc tgtaactaat ttggttctcc ggtcggggcc cttcgcgaag
    11101 cgatgggagc tctttctgat gttgccgaac cggcgcgacc gtcaggtcgc tgcaagagcg
    11161 ggcattctcc ggagcctgtc cacagggtag gcgatgaaga ggttatgtgt ttacaggatt
    11221 tattaaagga tctttaaaag agatacagaa ctggtgttta aaaggaacta cgcgccctga
    11281 aagaccgacg gcaggctcag aagatgctcc agcgtggcca tagtacgttc accgaagacg
    11341 tgtgcaacaa ccgtcctccg tatcctgtca tacgcgtaaa acaaccagcg ctggcgtgat
    11401 ttagcgccga cgtaacccca ctgttcgtcc atttccgcgc aaacaatgac gtcactgccc
    11461 ggttgtatgc gtgagtttac cgactgcggc
//
LOCUS       DADLSE010000120        11245 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLSE010000120 DADLSE010000000
VERSION     DADLSE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.120"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(228..303)
                     /locus_tag="JN692_003134"
     tRNA            complement(228..303)
                     /locus_tag="JN692_003134"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(268..270),aa:Phe,seq:gaa)
     gene            complement(409..1116)
                     /locus_tag="JN692_003135"
     CDS             complement(409..1116)
                     /locus_tag="JN692_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7265375.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLDALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1514..3649
                     /locus_tag="JN692_003136"
     CDS             1514..3649
                     /locus_tag="JN692_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7265376.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACSGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFVPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3698..4954)
                     /locus_tag="JN692_003137"
     CDS             complement(3698..4954)
                     /locus_tag="JN692_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7265377.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(5156..6235)
                     /gene="mltC"
                     /locus_tag="JN692_003138"
     CDS             complement(5156..6235)
                     /gene="mltC"
                     /locus_tag="JN692_003138"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7265378.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6300..6575)
                     /locus_tag="JN692_003139"
     CDS             complement(6300..6575)
                     /locus_tag="JN692_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7265379.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6603..7655)
                     /gene="mutY"
                     /locus_tag="JN692_003140"
     CDS             complement(6603..7655)
                     /gene="mutY"
                     /locus_tag="JN692_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7265380.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTSCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7816..8535
                     /gene="trmB"
                     /locus_tag="JN692_003141"
     CDS             7816..8535
                     /gene="trmB"
                     /locus_tag="JN692_003141"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7265381.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8535..8861
                     /locus_tag="JN692_003142"
     CDS             8535..8861
                     /locus_tag="JN692_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7265382.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGRICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            9045..9764
                     /locus_tag="JN692_003143"
     CDS             9045..9764
                     /locus_tag="JN692_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7265383.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9940..10986
                     /gene="ansB"
                     /locus_tag="JN692_003144"
     CDS             9940..10986
                     /gene="ansB"
                     /locus_tag="JN692_003144"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7265384.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            11103..>11245
                     /locus_tag="JN692_003145"
     CDS             11103..>11245
                     /locus_tag="JN692_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7265385.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDA"
BASE COUNT         2800 a         2758 c         2977 g         2710 t
ORIGIN      
        1 ccgtgcagta atgaatccgt tagccaggtg ctcatgtgga aatagtgtct attggggatt
       61 tgaagtttga cagaaaaggc cacagtgacc agaagggggc tacttagaag aaatgttcag
      121 gctggatgga agaggtaaag cagtgacatc agcattcaaa gtgaccggat atctaccaga
      181 accccagaaa gcaaaaaacc agcccgtagg ctggttcttc taaatagtgg tgcccggact
      241 cggaatcgaa ccaaggacac ggggattttc aatcccctgc tctaccgact gagctatccg
      301 ggcaacgggg cgcattaaac ctgattcgcc tcgtctcgtc aatcaaattt cttcaattta
      361 ctgcagactg cacaatctat catcactttg ccgctattgc acgcattgtc aggcaaacca
      421 ggcggtccag gcggcggaaa gcggcattgc cagaaggagt gctggaagca tgttgaccac
      481 cgggaacatt ttaatgccac agatgcgtaa tccggttgcc agcagcaata aaccgcctac
      541 ggcgctgaag tctgccatca tcgacggtgt ggtcagcggt aatatcagcg cggcagccca
      601 cgccagcgtt aactggatga tcagtaatgg gatactaatt accgacaccg caatacccag
      661 cgagcaggcg aagatcatcg ccgtaaagaa atcaagaaat gacttggcga ttaaaatact
      721 cggatcgccg gtcatccctt cgttcatcgc cccgaagatc ccggtgccgc tggcgcaaaa
      781 caggacaata atcgcgacat aattctgaat aaaagattca tgcgctggct tcttacgtga
      841 gtgacgaaac agattttgtg ctttggcgac tgctgtattg acacctttct ccagcaaaca
      901 aatttcgcca attagagcgt caagtagcgt agctaaaacc atcgctggaa ggttggcgca
      961 tttcacaacc agtaaaatac caatccccag cgatgccaga ccaaaaatag atgtcatgga
     1021 gatgcggata cgttccggta agcgttggct gagcagtgcg ccgagaacgc cacccagtaa
     1081 gactgcacta gcgttgataa aagggccgat gaccacaaga gttcctgtta gctgtattgt
     1141 ttgattttct tattatgaac tttttgcctt gttgatggct tgtctggtcg caaagtcctg
     1201 gcttgcacgc cttagcgaaa ggtgccatga ttgcgcgaat tttctcctct ctgtacggag
     1261 tttgcccgat gcacgccatc tccttacatt ctctcgctta tcgccgtttc gcgcgaaacg
     1321 tttccctttc tatgttactg ctcatgcggt gaagttaacg cacgctcact gcaggacaac
     1381 agtaaaatca gagcgtttct gcttttactg atgtctggcg gtcggagctg gtgaccagtt
     1441 tgacccatat ctcatggggc agggttttcc accttgtccg gtattcttac ttccccgaaa
     1501 cgggtttgcg cttatgaaat caatgaatat tgccgccagt agtgaactgg tatcccgact
     1561 ttcttctcat cgccgcgtgg tggcgttggg agatactgat tttacggacg tcgcggcagt
     1621 cgtcattacc gctgcggata gtcgcagtgg cattcttgcg ttgcttaagc gcaccggttt
     1681 tcatctaccg gtgtttttgt attccgaaca tgctgttgaa ttacctgcgg gcgttacggc
     1741 ggtaatcaac ggcaacgagc agcagtggct ggagctggaa tccgcagcct gtcagtatga
     1801 agagaatttg ctgccaccgt tttatgacac gctgacgcag tacgttgaga tgggcaacag
     1861 cacctttgct tgctctggac atcaacatgg tgcgttcttt aaaaagcatc ctgccggacg
     1921 ccatttttac gatttctttg gtgagaacgt ctttcgcgcc gatatgtgta acgctgacgt
     1981 aaaattgggc gatctgctta ttcatgaagg atcggcgaaa gatgcgcaga aattcgcagc
     2041 caaagtcttt catgccgata aaacctattt tgtgctgaac ggcacatcgg cagcgaataa
     2101 agtggtgacg aatgcgctgt taacgcgtgg cgatctggtg ctcttcgacc gtaacaacca
     2161 taagtcgaat catcacggcg cgctgattca ggcggggacg acgccggtct atctggaagc
     2221 ttcacgtaac ccgtttggtt tcattggcgg tattgatgcg cactgtttta atgaagagta
     2281 tctgcgccag caaattcgcg acgttgcgcc agaaaaagcc gacctgccgc gcccgtttcg
     2341 cctgacgatt attcagctgg gaacctatga cggcactgtc tataacgccc gtcaggtgat
     2401 cgataccatt gggcatctgt gtgattacat tctgtttgat tccgcgtggg tcggttacga
     2461 acagtttatc ccgatgatgg cggatagctc gccgctgctg ttagaactta acgaaaacga
     2521 tccggggatt tttgtgaccc agttggtgca caaacagcag gctggattct cacagacgtc
     2581 gcagatccat aaaaaagata accatatccg cggacaggcg cgtttttgcc cgcataagcg
     2641 gttgaataac gcctttatgc tccatgcttc taccagccct ttctatccgc tgtttgctgc
     2701 actggatgtt aacgccaaaa ttcatgaagg ggagagtggg cgtcggctgt gggctgagtg
     2761 cgttgagttg ggtattgagt cgcgcaaggc tattcttgcg cgctgtaagc tgttccgccc
     2821 gtttatcccg cccgttgttg atggcaaatt gtggcaggat tatccgacat cagtgttagc
     2881 cagcgaccgc cgttttttca gttttgtgcc gggggcgaag tggcacggct ttgaaggata
     2941 tgccgcggat cagtattttg ttgatccgtg caagctgtta ctcactacac caggtatcaa
     3001 tgccgaaacc ggcgaatata gcgactttgg cgttccggcg acgattctgg cgcactatct
     3061 gcgtgagaac ggcattgtgc cggagaagtg cgatctcaac tccattctgt tcttattaac
     3121 tccggcggaa agccacgaga agctggcaca actggtggcg atgctggcgc aatttgaaca
     3181 gcatattgag gatgactcgc cgctggctga ggtgttgccg agcgtttata acaaatatcc
     3241 ggtgcgctat cgcgactaca ccctgcgcca gttgtgtcag gagatgcacg atctgtatgt
     3301 cagtttcgac gtcaaagacc tacaaaaagc gatgttccgc cagcagagtt tcccgtcagt
     3361 ggtgatgaac ccccaggatg cgcatagcgc ttatattcgc ggtgaagtgg agttggtgcg
     3421 gattcgtgat gccgaagggc gaattgcggc agaaggggcg ttgccttatc cacctggcgt
     3481 gctttgcgtg gtacccgggg aagtctgggg tggggcggtt caacgttatt tccttgcgct
     3541 ggaagaaggc gtgaatttgt tgccgggatt ttcgccggag ttgcaaggtg tctatagcga
     3601 aaccgatgcg gatggcatga aacgattgta cggttatgtg ttgaagtaag aataaaaaaa
     3661 cgggtcacct tctggcgacc cgtttttctt tgcgtaatta gtggctaacc gtctgtgtgc
     3721 ctgtcgggac acgaacgtgt ttatatttga acatcgccat gaacgcgaag gccagaacca
     3781 cggaataacc agcgaaaatc aaccatacgg tctgccagtc ggtaatgccg ttttgggtgt
     3841 acatctcaac aactttaccg ctcacgatgc cgccgaggat acagccgaag ccgttagtca
     3901 tcatcaggaa catcccttgt gcactggcgc gaattgccgg gctaacttct ttttcgacaa
     3961 acaccgaacc agagatgttg aagaagtcga atgcgcaacc gtaaacgatc atcgacagta
     4021 ccagcagtac agtaccgaac ggagtcgggt cgccgtaagc aaacagcgca aaacgcagga
     4081 tccacgccac aatactgatc atcattacgt tcttaatacc gtagcggctt aagaagaacg
     4141 ggatggtcag aatgaacagg gtttcagaga tctgcgaaat cgacatgatg attgacgcat
     4201 gctgcacgat gaagctgctg gcaaacatcg gatctttgtc gaagctgtgc aggaaggtat
     4261 taccgaacat gttggtaatc tgcagttccg cgcccagcag cattgagaag atgaagaaga
     4321 ttgccatacg cttgttttta aacagcgcga atgcatcgag gcccagcagg gttgtccagc
     4381 tctgattcgc ttgctgttta gcaaccggaa tatgcggcag agtcagggta aacagaacca
     4441 gaatggcgga aagtgctgcg ccaatataca gctgcatgtg gcttaattcg aagccagaca
     4501 ggctcaccac ccacattgcc atgataaagc cgatggtgcc ccagatacgg attggcggga
     4561 agtcagtaac gatatccatc ccggcatttt gcaggcgata gtaagagatg gtgttgatta
     4621 acccaagcgt tggcatataa gcaaacgagt taatcaatat cacaaggaac atcgcttccg
     4681 gcgtcgtgac ctgtgccgcc atgaacagcg tgatagcgcc aatggtgtgg caaatggcat
     4741 atacccattt cgcacttaac catttgtcgg ccacaatccc cagcagcgca ggcataaata
     4801 ccgcggcgat acccagtgag ctataaactg cgccaataga agcaccgtca aacttcaggg
     4861 taacaaacat ataggagccg agggtcgtca gccaacttcc ccacagacag aactgcagaa
     4921 aagagaggat tttcagctgc agcttaagat tcatgttaat ttcctcacat cgtgatgcgg
     4981 atgagtgttt gaaaaagcac atttgaccgt gcgagatgat gggattctat caacttcgcg
     5041 gactggtttt ttgttacctg tggcaaataa ttgcaaacat tcccaatttg caaaacgaaa
     5101 ttgtgatgtg gataacattt ttgcccctga gcatcgtcag gggcggttaa tggaattatc
     5161 ggcggcggta ggatttttgc gcggtattca ctttataaag ataacggcga gattccgcag
     5221 agggatggcg ggtcgtcagc gtctgataaa catcgcccgg cgtcatggtg ttaataatat
     5281 tggcagcctg aatcttatca ttcgaaaaga ctcgcagcac gctgcctgcg ccgccgttat
     5341 aggcggtgat gacggcataa cgccgcgatg ttgggttatc aattccgccg agataaacat
     5401 tgttcagcat cgccagatac gcggtgccgg tatcaatatt gctggcagga tcaaacaaga
     5461 aactgcggct cggcgtgccg gatttcccct gcgagcggaa cacatctttc ccggcagtat
     5521 tttgtaccac ctgcattaat cccagcgcat cggaacggct gaccgcatac gggttaaagg
     5581 aagattcagt ctgcataatt gccagaatca gcgactcatc aacgccatat ttccgtgacg
     5641 cctggcggac catgccgaga tatttgtgcg cacgtttatc aaggtggttc ggcaccatgt
     5701 taatggtgac gctgtagatg atacgtagcc cgttgctgcg gcttttcaga cggtttttca
     5761 gcagataatc cgcgaagttg cttgcgcgac cttcccagcg aatcggctgc ccggtgttgt
     5821 ccaccacctg accgtaaagg aaaggttctt tcgaaattgt aatatcatca acgtcggaat
     5881 agagatcgac cgaactcgga tcgtcaccca tcagcaacgt tttgataatt gcccggcgca
     5941 aatgcgcggc aggttctgtc ccggcgatgg tttcgatagt aatcgtaccg tcatcgaagt
     6001 tgatgtggct gcgggtctga tattgatcgg tgtatttcac gtagtcctta ggaccggcga
     6061 tcaccacctc tttgaagccc cagatgttct caatattgtg ggcaaattgc cccatcagaa
     6121 tatcaaaacc gttggtatct ttgacccagg cttcgttata ggtatcgcct tttttggtcg
     6181 tcgaacagga gatgagcaac ggcgcaatca aagccagcgc gagatatttt ttcatcattc
     6241 cgggtgcgtg ttgtgtttgt ttttatgcaa gctgccggag gcgtgctccg gcactgtttt
     6301 tattttttat cttccggcgt atagccctcg atatgcacct ctttaccctc gaacaggaag
     6361 ttgaccatct cctgctccag cagcttgcgg tgctcggcat tcatcatgtt gagtttcttt
     6421 tcattaatca gcatggtttg tttgtgctgc cactgcgccc aggcttcttt ggagatctcg
     6481 ttatagatgc gttttcccag ctcgccgggg tacagctgaa aatcctgacc ttctgcttca
     6541 cgttgcagga aagtacaaaa aatcgttctg ctcataaatc atcctcttta tcgactcacg
     6601 cgctaaaccg gcgcgccagt gcgtaactgc tgtaacaaac gctccacggg agccgccagg
     6661 ccaactgacg gcggttgcgc taagttatac cagagcgcat tgccttcatc catgcagctg
     6721 gtgaatgacg acacgggaag ccacataggc acaatatcta agtggaaatg gctgaaggta
     6781 tggcgaaacg cggtcagttg cgtcaggtta tcggcagcaa tctgccgttg tgccagccaa
     6841 tgccgcaaac tttcttcgtc ggcaaactgc gggaaacagt ataaaccgcc ccacaatccg
     6901 ctcggcggac gctgcgccag caatacttca tcttcgtgct gtaacaggag aaagtagccg
     6961 gtgcgctccg gcagcgtctg tttcggtttt ttgcccggat aaagcgacca gctattgttg
     7021 gtggcggcaa tacatccgtt ttgtagcgga cagagcgaac atttcggctt cgaacgcgtg
     7081 caaatcatcg cgcccaaatc catcatcgcc tgattaaacc gttccacgcc gaccgcgggc
     7141 gtcacctgct cgctcaaact ccatagttta ttctcgacct cttttttccc aggccagccg
     7201 cttacagcat agcagcgcgc cagcacgcgt ttgacgttac cgtcgagaat cggaaagtgc
     7261 ttacccagag aaagcgagag aatcgcgcct gcggtggaac gcccgacgcc cggtaacgcc
     7321 gcgacttctt caaaggtttc cgggaattta ccgccgtgta aggtcgccac ttgttgtgcc
     7381 gctttatgca ggttgcgcgc gcgggcgtaa tagccaagcc cggtccacaa gtggagaact
     7441 tcgtcgagcg gcgcattggc gagatcggtc accgtcggga agtgcgccat aaagcgttca
     7501 aaatagggga taacggtcgc aacctgagtt tgttgcaaca tcacttctga gagccatact
     7561 ttgtagggcg tcttgtcaat ttgccagggc agcgttttcc gcccgtattt atcgtaccag
     7621 tccagaacct gggctgaaaa ttgcgacgct tgcatggtca ccgacttcac tgttgttggg
     7681 ggcaagattg cagcacagcg acggcagggt gtaaaccgga actttccgca gccacggcct
     7741 tatcatgctt gcatccggca actaactttg gataatgccc gttttcagaa cactttcaca
     7801 agcgactaaa tctttatgaa aaacgacgtc atttcaccgg aatttgatga aaacggccgc
     7861 ccactgcgcc gtatccgtag ttttgtgcgc cgccaggggc gactgaccaa aggccaggaa
     7921 catgcgctgg aaaactactg gccggtgatg ggcgttgagt tcagtgaaga tatgctggat
     7981 ttccccgcgc tttttggccg tgaagcgccg gtgacgcttg agattggttt tggcatgggg
     8041 gcgtcgctgg tggcaatggc taaagatcgc cccgagcagg acttcctcgg cattgaagtg
     8101 cattcaccgg gcgttggtgc gtgcctggct tctgcgcatg aagaaggttt aagcaacctg
     8161 cgcgtgatgt gtcacgatgc ggttgaagtg ctgcataaaa tgattcctga caattcattg
     8221 cgcatggtgc agctcttttt ccctgacccg tggcacaaag cgcgccataa taaacgccgt
     8281 atcgttcagg tgccgtttgc cgaactggta aaaagcaaac tgcagctggg gggcgtattc
     8341 catatggcga ccgactggga accttatgcg gaacatatgc ttgaagtgat gtcttctatt
     8401 gacggttata aaaacctgtc agagagcaat gattacgtac cgcgtccggc atcacgtccg
     8461 gtgacgaaat ttgaacaacg tggtcatcgt cttggtcacg gagtatggga cttaatgttc
     8521 gagagggtga aataatggca aagaaccgta gccgtcgtct gcgtaaaaaa atgcacatcg
     8581 acgaattcca ggaattagga ttttcggtgg catggcgatt cccggaaggt acatcggaag
     8641 aacagattga taaaatcgtt gatgacttta ttaacgaggt tatcgaaccg aacaaactgg
     8701 cctttgacgg cagcggttat ctggcctggg aaggtcggat ctgcatgcag gaaatcggca
     8761 aatgcaccga agaacatcag gcgattgtgc gtaagtggct ggaagagtgc aaactggatg
     8821 aggtacgcac cagcgaactt ttcgacgttt ggtgggacta agaaagcata cgggcgatga
     8881 caaatgcaaa actgcctgat gcgctacgct tatcaggcct acagagatgc acgatcgagt
     8941 aggcgggata aggcgtttac gccgcatccg gcatggaaaa cgcgtacttt gttatcaatc
     9001 tggggccagc atatgctggc ctgatttgtt cttgagggaa gactatgatg cgcaaaatgc
     9061 tgctggcggc agcactttca gtgacggcaa tgaccgctca cgccgactac cagtgcagcg
     9121 tcacgccgcg tgacgatgtg attgtcagcc cgcaaaccgt gcaggtgaag ggcgaaaacg
     9181 gcaatctggt gatcacgcca gacggcaacg tgatgtataa cggtaagcaa tattccctga
     9241 atgccgccca gcgcgagcag gcgaaggatt atcaggctga actacgtagc acgctgccgt
     9301 ggattgatga aggcgcgaaa agccgcgtcg agaaagcccg tattgcgctg gataaaatta
     9361 tcgttcagga gatgggcgac agcagcaaga tgcgcagccg tctgaccaaa cttgatgcgc
     9421 agcttaaaga gcagatgaat cgcattatcg aaacgcgcag cgatggcctg acgttccact
     9481 ataaagccat tgatcaggtt cgcgccgaag gccagcaact agtgaatcag gcaatgggcg
     9541 gaattttaca ggacagcatt aatgaaatgg gcgcgaaagc ggtgctgaaa agcggcggta
     9601 acccattaca gaatgtgctg ggaagcttgg gcggtctgca atcctcaatc caaaccgagt
     9661 ggaaaaagca ggaaaaagat ttccagcagt ttggcaaaga tgtttgtagc cgcgttgtga
     9721 ctctggaaga tagccgcaaa gccctggtcg ggaatttaaa ataatcctct attttaagac
     9781 ggcataatac ttttttatgc cgtttaattc ttcgttttgt tacctgcctc taactttgta
     9841 gatctccaaa atatattcac gttgtaaatt gtttaacgtc aaatttccca tacaaagcta
     9901 agggataatg cgtagcgttc acgtaactgg aggaatgaaa tggagttttt caaaaagacg
     9961 gctcttgccg cactggttat gggttttagt ggtgcagcat tggcattacc caatatcacc
    10021 attttagcaa ccggcgggac cattgccggt ggtggtgact ccgcaaccaa atctaactac
    10081 acagcgggta aagttggcgt agaaaatctg gttaatgcgg tgccgcaact aaaagacatt
    10141 gcgaacgtta aaggcgagca ggtagtgaat atcggctccc aggacatgaa cgataatgtc
    10201 tggctgacac tggcgaaaaa aattaacacc gactgcgata aaaccgacgg cttcgtcatt
    10261 acccacggta ccgacacgat ggaagaaact gcttacttcc tcgacctgac ggtgaaatgc
    10321 gacaaaccgg tggtgatggt cggcgcaatg cgcccgtcca cgtccatgag cgcagacggt
    10381 ccattcaacc tgtataacgc ggtagtgacc gcagctgata aagcctccgc taatcgtggc
    10441 gtgctggtgg tgatgaacga caccgtactg gacggtcgcg atgtcaccaa aaccaacacc
    10501 accgacgtag cgaccttcaa gtctgttaac tacggtcctc tgggatacat tcacaacggt
    10561 aagattgact accaacgtac cccggcacgt aagcacacca gcgatacgcc attcgatgtc
    10621 tctaagctga atgagctgcc gaaagtcggc atcgtttata actacgctaa cgcatccgat
    10681 cttccggcta aagcactggt agatgcgggc tatgatggca tcgttagcgc tggtgtgggt
    10741 aatggtaacc tgtataaatc cgtgttcgac accctggcaa ccgccgcgaa aaacggcact
    10801 gcagtagtgc gttcttcccg cgtaccgacg ggtgctacca ctcaggatgc tgaagtggat
    10861 gatgcgaaat acggcttcgt cgcctctggc acgctgaacc cgcaaaaagc gcgcgtcctg
    10921 ctgcagctgg ctctgacgca aaccaaagat ccgcagcaga tccagcagat tttcaatcag
    10981 tactaatcac ctcgtcccgg catcgtgccg gggctttttc acttcagact tacgtccatt
    11041 gccaattttc attaccctaa tgataatcac cggaataaat tattccgcgc gagggttttc
    11101 gggtgaaaaa gcaatggatt gttggcacgg cgctgcttat gttgatgact ggtaatgcct
    11161 gggcagatgg cgaaccgcca actgaaaata tcttaaaaga tcaattcaaa aagcagtatc
    11221 acggcattct caagcttgat gccat
//
LOCUS       DADLSE010000121        11142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLSE010000121 DADLSE010000000
VERSION     DADLSE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.121"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN692_003146"
     CDS             complement(327..1991)
                     /gene="glnS"
                     /locus_tag="JN692_003146"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7265386.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN692_003147"
     CDS             complement(2194..4140)
                     /gene="nagE"
                     /locus_tag="JN692_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7265387.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAVVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAVGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISLVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4473..5273
                     /gene="nagB"
                     /locus_tag="JN692_003148"
     CDS             4473..5273
                     /gene="nagB"
                     /locus_tag="JN692_003148"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7265388.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5333..6481
                     /gene="nagA"
                     /locus_tag="JN692_003149"
     CDS             5333..6481
                     /gene="nagA"
                     /locus_tag="JN692_003149"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7265389.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6490..7710
                     /gene="nagC"
                     /locus_tag="JN692_003150"
                     /gene_synonym="nagR"
     CDS             6490..7710
                     /gene="nagC"
                     /locus_tag="JN692_003150"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7265390.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLTSEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7758..8510
                     /gene="nagD"
                     /locus_tag="JN692_003151"
     CDS             7758..8510
                     /gene="nagD"
                     /locus_tag="JN692_003151"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HAY7265391.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEEAVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8907..10571
                     /gene="asnB"
                     /locus_tag="JN692_003152"
     CDS             8907..10571
                     /gene="asnB"
                     /locus_tag="JN692_003152"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7265392.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10951..11027
                     /locus_tag="JN692_003153"
     tRNA            10951..11027
                     /locus_tag="JN692_003153"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10985..10987,aa:Met,seq:cat)
     gene            11036..11120
                     /locus_tag="JN692_003154"
     tRNA            11036..11120
                     /locus_tag="JN692_003154"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11070..11072,aa:Leu,seq:tag)
BASE COUNT         2784 a         2864 c         2810 g         2684 t
ORIGIN      
        1 cagcaagatg aaagcggtta cattttattt aattatgttt tgtgatttta aatttatttt
       61 atggataaat tgcgaagcgg aattaattcg cgaagaaaag cagaaaaaac gccgcagagc
      121 gcggcgtttg ggattgtttg ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc
      181 tacaaaagca cgcaaattca atatattgca gagatcatgt aggcctgata agcgaagcgc
      241 atcaggcaat ttagcgtttg aaatttattg tctatttatt gccggatgcg acgtaaatgc
      301 ctcacccggc ttggtagaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
      361 ccaacggtgc ggttaaatac cggtttttcg gccgtggaat ggcggctgtc gaggcagaag
      421 taaccttcac gctcaaactg gaatgcttta cccgctaccg catctttcag cgacggttca
      481 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      541 gcacctgggt taggcacgct gaacagacga tcatacaaac ggatttcaac cggcagcgca
      601 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
      661 cttaaggtgt cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc
      721 tccacgcgtt cagccttaat gacgtaagca ttacgcagac gcacttcttt acccagcacc
      781 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca
      841 ccgctaaacg gcacctgacg gctccccatt tccggtttgt tcggatggtt cggcatggtg
      901 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
      961 attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc
     1021 tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc
     1081 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
     1141 tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac agtgtattcc
     1201 agattcaggc gcgagaattc gtactggcgc ggatgaacag gaatggtgat gttatccagt
     1261 acccagtcgt acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1321 ccttccagcg catcgctgat gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg
     1381 ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc gcgcattacg
     1441 ataaatggcg acgccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
     1501 ccggcacgca ttttttcgaa cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg
     1561 ctgtttttac ccggctgcgt cagggtaccg cggtattcgc ggatctgttc cggtgtcagt
     1621 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     1681 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
     1741 tttttgatcg aatcgacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     1801 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     1861 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     1921 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
     1981 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     2041 agcgggacag gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg
     2101 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttgtcat tgttggatgc
     2161 gacgctgccg tgtcgcatca ggcataaagc agattacttt ttgatttcat acagcggtgt
     2221 ttgacccgcc acaacatggc cctgagcttt aatgatcaag ccactgaaat cgtcgatatt
     2281 gctgcaaacc accaggctaa tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat
     2341 ttccagaatc ggttgccctg cgcttacctg cgcaccctct tccaccagac gtttaaagcc
     2401 tttaccttcc agcgctacgg tgtcgatacc catatggacg acgatctccg cgcctttttc
     2461 ggtttccagg cagaacgcgt ggttggtgtt gaagattttc acgattgtcc ctgcggctgg
     2521 tgatacgacg attttatctg tcggtttcac cgccacaccg tcacccaccg ctttgctggc
     2581 gaatgcttcg tcaggaacct gatccagtgc cacgacatca ccggtaatcg gcgataccag
     2641 ctccgcgata gataccgcgt ttggtacagc ctgcggtttt gctacaggcg cggcagttgc
     2701 cggagttgct tcagctgacg cagcggctac cggaccacgg gcaacgactt tcttcatcgc
     2761 atcgccgatg gattctgctt tcgcgccaac aatcacctga atagtctgtt tgttcagttt
     2821 cactacacca gaagcaccca gacgtttaca catcgtatcg ttgacgcggg cagagtcagc
     2881 cacggtcagg cgcagacggg taatacaggc atcaatcgct ttcaggttgt cagtaccgcc
     2941 aaccgcagca atatagttgg ttgccagttg agtcagacct tcttcagtat tgctgttggc
     3001 ttcttcagta acgatctcgt cttctttatc ttcacgaccc ggcgttttca ggttgaacat
     3061 gcggataacc aaactgaaca ccacgaagta gatagcgaag aagataacgc ccatcaccag
     3121 cagcatccag acgttctggc tggcggccgg caggttatac atcaacgcgt agtcgatagc
     3181 ccccgcagag aaagagaagc cggcatggat acccagcagc gttgccacaa acaggctgat
     3241 accggtcagc agtgcgtgca ggaggtacag cagcggcgca aggaacatga acaggaattc
     3301 cagcggctca gtcacaccgg tcaggaacgc agtaacagca acagaaagca gcatcccgcc
     3361 aaccatcgga cgacgctctt tcggtgctgc gaagtacatc gccagcgccg cacccggcag
     3421 accgaacatc atgatcggga agaagccgga catgaacatc cccgcggtgc cgtcaccagc
     3481 gtagaaacgg ttgatgtcgc cgtggaaaac cgtacccacc gcgttggtga attcaccaat
     3541 ctggaaccag gcgatggtgt tcagcacctg atgcagaccg gttgggatca gcagacggtt
     3601 gatgaaacca aagataccgg aacccagcgc gcccgcagaa acgatccact cgccgcctgc
     3661 atggatagcg tgctgtaccg gcggccagac gtaaccaaaa atggccgcca gcaccagaca
     3721 gaagaagccg gtggcgatcg gcacaaagcg tttgccgccg aagaagctca ggaagtccgg
     3781 cagtttaata tcggaccaac ggttataggc tgcaccacca accagaccgg taatgatacc
     3841 cgccagtaca cccatgttaa tttctgggtt gatggtcacc accgctttgg ttaacacaaa
     3901 gtaacctacc gcacccgcca gcgccgccgc acctgcgctg tctttcgacc agctggatgc
     3961 cacaccgatg gcgaagatta atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat
     4021 aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc aacagcgccg ccaccggcag
     4081 caccgcgata gggagctgta acgccctacc gagtcgctgg aaaaaaccta aaatattcat
     4141 cttattcccc ctacgagaac cctatttggc tcgtttaaag ccgtattttt attttgctgc
     4201 aaattttact gccgatgttc tgtaatcaga ttgttagatc atctgctaca gagtgtgtga
     4261 aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt gagttttgtc accaaatatc
     4321 gttatcatca ctccctttta ctggctaaac cagaaaactt attttatcat tcaaaaaatc
     4381 aagtcggatt gacgcctgtc tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc
     4441 aacggcttac attttactta ttgaggtgaa taatgagact gatccccctg actaccgctg
     4501 aacaggtcgg caaatgggct gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa
     4561 ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg cacgccgatg accacctata
     4621 aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt taagcacgtt gtcaccttca
     4681 acatggacga atatgtcggt ctgccgaaag agcatccgga aagctactac agctttatgc
     4741 accgtaattt cttcgatcac gttgatattc cagcagaaaa catcaacctt ctcaacggca
     4801 atgccccgga tatcgatgcc gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa
     4861 aaattcatct gtttatgggc ggtgtaggta acgacggtca tattgcattt aacgaaccgg
     4921 cgtcttctct ggcttctcgt actcgtatca aaaccctgac tcatgacact cgcgtcgcga
     4981 actctcgttt ctttgataac gatgtcaatc aggtgccaaa atatgccctg actgtcggtg
     5041 ttggtacact gctggatgcc gaagaagtga tgattctggt gctgggtagc cagaaagcac
     5101 tggcgctgca ggccgccgtt gaaggttgcg tgaaccatat gtggaccatc agctgtctgc
     5161 aactgcatcc gaaagcgatc atggtgtgcg atgaaccttc caccatggag ctgaaagtta
     5221 agactttaag atatttcaat gaattagaag cagaaaatat caaaggtctg taattgttat
     5281 ccctgccctg gttccttgct cagggcaata ttttttaaaa tcgggggtca gaatgtatgc
     5341 attaacccag ggccggatct ttaccggcca cgaatttctt gatgaccacg cggttgttat
     5401 cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca
     5461 acgttctctg aacggggcca ttctctcccc cggttttatc gatgtgcagt taaacggctg
     5521 cggcggcgta cagtttaacg acaccgctga agcggtcagc gtggaaacgc tggaaatcat
     5581 gcagaaagcc aatgagaaat caggctgtac taactatctg ccgacgctta tcaccaccag
     5641 cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa
     5701 tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca
     5761 taatccgaat tttgtgcgta agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc
     5821 cgacgtcatt accaaagtga ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa
     5881 actggcaaat gccgggattg tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc
     5941 aaaagccggt ttccgcgcgg ggattacctt tgccacccat ctgtacaacg cgatgccgta
     6001 tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc gacgaagctg acatttattg
     6061 cggtattatc gctgatggcc tgcatgttga ttacgccaac attcgtaacg ctaaacgcct
     6121 taaaggcgac aaactgtgtc tggttaccga cgccaccgcg ccagcaggtg ccaacattga
     6181 acagttcatt tttgcgggta aaacaatata ctaccgtaac ggactttgtg tggatgagaa
     6241 cggtacgtta agcggttcat ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca
     6301 ttgcggtatc gcactggatg aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat
     6361 tgacgttgaa aaacgtctcg gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt
     6421 cacacctgat tttaaaatca ccaagaccat cgttaacggt aacgaggtcg taactcaata
     6481 agagaaagta tgacaccagg cggacaagct cagataggta atgttgatct cgtaaaacag
     6541 cttaacagcg cggcggttta tcgcctgatt gaccagtacg ggccaatctc gcggattcag
     6601 attgccgagc aaagccagct tgcccccgcc agcgtaacca aaattacgcg tcagcttatc
     6661 gaacgcgggc tgatcaaaga agttgatcag caggcctcca ccgggggccg ccgcgctatc
     6721 tccatcgtca ccgaaacccg caatttccac gcaatcggcg tacggcttgg tcgtcatgac
     6781 gccaccatca ctctgtttga tctcagcagc aaagtgctgg cagaagaaca ttacccgctg
     6841 ccggaacgta cccagcagac gctggaacat gccctgctga atgccattgc tcagtttatt
     6901 gatagctacc agcgcaagct gcgcgagcta atcgccattt cggtgatcct gccagggctt
     6961 gttgacccgg acagcggcaa aattcattac atgccgcata ttcaggtaga aaactggggg
     7021 ctggtagaag cactggaaga acgttttaaa gtgacctgtt tcgttggtca cgatatccgt
     7081 agtctggcgc tggcggagca ctacttcggt gcaagtcagg attgcgaaga ctccattctg
     7141 gtgcgtgtcc atcgcggaac cggggccggg attatctcta acgggcgcat ttttattggc
     7201 cgcaacggta acgtcggtga aattggccat attcaggtcg aaccgctggg tgaacgctgc
     7261 cactgcggca actttggctg cctggaaact atcgccgcca acgctgccat tgaacaacgg
     7321 gtgttgaatc tgttaaagca gggctaccag agccgtgtgc cactggacga ctgcaccatc
     7381 aaaactatct gcaaagccgc gaacaaaggc gatagcctga cctcggaagt gattgagtat
     7441 gtcggtcgtc atctgggtaa aaccatcgcc attgctatca acttatttaa tccgcaaaaa
     7501 attgttattg ctggtgaaat caccgaagcc gataaagtgc tgctccctgc tattgaaagc
     7561 tgcattaata cccaagcgct gaaggcgttt cgtactaatc tgccggtggt acgttctgag
     7621 ctggatcacc gctcggcaat cggcgctttt gcgctggtaa aacgcgccat gctcaacggt
     7681 attttgctcc agcatttgct ggaaaattaa tgtgctttta tagtggcgct tattgttgtc
     7741 aatattctgg gtagtccatg accattaaaa atgtaatttg cgatatcgac ggcgtgctga
     7801 tgcacgataa cgtcgccgta ccgggtgcag cggaattttt gcacgggatt atggataaag
     7861 gcctgccgct ggtgttgctg accaactatc cttcgcagac tgggcaagat ctggcgaacc
     7921 gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt ttatacctct gcgatggcga
     7981 ccgccgattt cctgcgtcgc caggaaggca agaaagcgta tgtggtgggc gaaggcgcac
     8041 tgattcatga actgtacaaa gccggtttca ctattaccga tgtgaaccct gatttcgtga
     8101 ttgttggcga aacacgttcc tacaactggg acatgatgca taaagcagcc tatttcgtcg
     8161 ctaacggtgc acgctttatc gccaccaacc cggacaccca cgggcgcggt ttttatcccg
     8221 cttgtggcgc gttgtgtgca gggattgaga aaatctccgg gcgcaaaccg ttctatgttg
     8281 gtaagcccag cccgtggatc atccgcgcgg cattaaacaa aatgcaggcg cattcggaag
     8341 aagcagtgat tgtcggcgat aacctgcgta ccgatattct ggcgggcttc caggccggtc
     8401 tggagacgat tctggtgctt tctggtgttt catcgctcga cgatatcgac agtatgccgt
     8461 tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga cgttatctga ataccaccca
     8521 cgtctgcgga cgtggttggc tctgatgaca aacgccaaac tgcctgatgc gctacgctta
     8581 tcaggcctac gcagctcctg caatatattg aattttcatg cttttgtagg ccggataagg
     8641 cgttcacgcc gcatccggca tgaacaaatc gcactttatc agcaatttgg cccccgtaaa
     8701 atataaccat tcaataaaac ccgctaaaaa ttatcgattc atcaataaat acatcctcac
     8761 accagaccct ttttcaccaa tcgacaatca ccattacgtt ttttattttt gcactgccaa
     8821 atagcttgcg cacagtactg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     8881 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
     8941 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
     9001 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgctcac gaacgtctgt
     9061 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa acccacgtac
     9121 tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg cgctgaatat ggcgatcgtt
     9181 atcagttcca gacagggtct gactgtgaag tgatcctcgc gctgtatcag gaaaaagggc
     9241 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
     9301 atgcctacct gattggtcgc gaccatctgg ggatcatccc actgtatatg gggtatgacg
     9361 aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct ggtgccagtt tgccgcacga
     9421 ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt
     9481 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
     9541 tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgatgtg ccttacggtg
     9601 tgctactttc tggtggtctg gattcctcaa ttatttccgc tatcaccaag aaatacgcag
     9661 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcagtta cactcctttg
     9721 ctgtaggtct gccgggttca ccggatctta aagcagccca ggaagtggca aaccatctgg
     9781 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
     9841 tgatttacca catcgaaact tatgatgtga ccactattcg cgcttcaaca ccgatgtatt
     9901 taatgtcgcg taagatcaag gcgatgggca ttaaaatggt gctgtccggt gaaggttctg
     9961 atgaagtgtt tggcggttat ctttacttcc ataaagcgcc caacgctaaa gaactgcatg
    10021 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
    10081 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
    10141 tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
    10201 tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc
    10261 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
    10321 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccgacct
    10381 caaaagaagc gtatctgtac cgcgagatct ttgaagaact gttcccactt ccgagtgccg
    10441 ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc agctaaagcg atcgaatggg
    10501 atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    10561 cgtataagta agaaatgcgg tactgccccg gagaaatccg gggctgtttt agttattgcg
    10621 ctgcttgttg attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    10681 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    10741 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    10801 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    10861 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    10921 aacgccgata aggtatcgcg aaaaaaagat ggctacgtag ctcagttggt tagagcacat
    10981 cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccatct tttttgcggg
    11041 agtggcgaaa ttggtagacg caccagattt aggttctggc gccgcaaggt gtgcgagttc
    11101 aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac gg
//
LOCUS       DADLSE010000122        10886 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLSE010000122 DADLSE010000000
VERSION     DADLSE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.122"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..184
                     /locus_tag="JN692_003155"
     CDS             <1..184
                     /locus_tag="JN692_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7265393.1"
                     /translation="LRPRNPETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRPV
                     TGSLLKRIRNGKKAKS"
     gene            162..1235
                     /locus_tag="JN692_003156"
     CDS             162..1235
                     /locus_tag="JN692_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7265394.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     VSEAIQANIELLSENRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLNDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYIEIYHAAEPLPIWLRLAMDLAVITGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKIAIPLTLTIDALNISLVDTLQKCREASGSETVIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIDK"
     gene            complement(1293..1853)
                     /locus_tag="JN692_003157"
     CDS             complement(1293..1853)
                     /locus_tag="JN692_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7265395.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGNGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLHV"
     gene            complement(1850..3538)
                     /locus_tag="JN692_003158"
     CDS             complement(1850..3538)
                     /locus_tag="JN692_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7265396.1"
                     /translation="MSGEKKTKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(3687..6314)
                     /gene="gyrA"
                     /locus_tag="JN692_003159"
     CDS             complement(3687..6314)
                     /gene="gyrA"
                     /locus_tag="JN692_003159"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7265397.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYYTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6461..7183
                     /gene="ubiG"
                     /locus_tag="JN692_003160"
     CDS             6461..7183
                     /gene="ubiG"
                     /locus_tag="JN692_003160"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HAY7265398.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPSGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(7311..10886)
                     /gene="yfaL"
                     /locus_tag="JN692_003161"
                     /pseudo
     CDS             complement(7311..10886)
                     /gene="yfaL"
                     /locus_tag="JN692_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2613 a         2969 c         2663 g         2641 t
ORIGIN      
        1 gttacgtcca cggaacccgg aaacggttcg ccgctgggta cgcgagtgca ggatattccc
       61 tcctccggtt aaggatggaa gagagtatct gttccacgaa tcagcggtaa aggttgactt
      121 aaatcgacca gtaacaggta gccttttgaa gaggatcaga aatgggaaga aggcgaagtc
      181 atgagcgccg ggatttaccc cctaaccttt atataagaaa caatggatat tactgctaca
      241 gggacccaag gacgggtaaa gagttcggat taggcagaga cagaaggata gcagtttcag
      301 aggctattca ggccaatatt gagttgctat ctgagaacag gcgtgagtca ctgatagaca
      361 ggattaaagg cgctgacgca atcactcttc atgcgtggct tgaccgatat gaaacaatcc
      421 tcagcgagag gggtatcaga ccgaaaactc tactcgacta cgccagcaaa atcagggcga
      481 ttcgaagaaa attgccggac aaaccgctca atgacatatc aacgaaagag gtggcagcaa
      541 tgctaaacac ctacgtagca gaaggtaaag cggcttccgc aaaattaatc aggtcaaccc
      601 ttgttgacgt ttttcgtgaa gcaatagccg aggggcatgt ggctacgaat ccggtaacag
      661 caacccgcac agcaaagtca gaagtaaggc gctcaaggct tacagctaat gagtatatcg
      721 agatttacca tgcagccgaa cctctcccta tctggctgag gctggcaatg gatttggccg
      781 tcattacagg gcagagagtg ggcgatttat gcagaatgaa gtggtcagac ataaacgaca
      841 accatcttca cattgagcag ggtaaaacag gagccaagat tgccattccg ctgacgctaa
      901 cgattgacgc gctcaacatc tcactggttg atacactaca gaaatgcagg gaggccagcg
      961 gcagtgaaac tgtaatcgcc tcaacgcatc acgaaccgct ttccccgaaa acagtatcga
     1021 agtattttac aaaggcgaga aatgcctccg gactctcatt tgatggagac ccgccaacat
     1081 tccatgaact gcgtagcctg tcagcgaggc tataccggaa ccagattggc gataaatttg
     1141 ctcaacgtct tctcggacat aaatcagatt caatggcggc gcggtatagg gacagccgtg
     1201 gacgggaatg ggacaaaatt gaaatcgaca aatgatttta ttttgaccaa taatgacctg
     1261 ctcatcttaa cagattgata attaatgcaa ttttaaacat gcaacttcca tgtcataagt
     1321 tgttgcagac tgactgcgcg cattccgttg tcagttttcg tggcgcttcc ggtgtggtag
     1381 atgacgtaac gccccatcca gaccattaag tgctgggcat cgccctgatc gaaaaaaatc
     1441 atatcgccag gcagcgcctg gtttatgtct tggccaataa actgactgtt gtactgaatc
     1501 aaattaatcg cggtcacgta ggggccgttt ttcccgttcc cctgattcca gttttgcgcc
     1561 agttgacgct gttcaggtgt tagcgtcatc tctggcggca aatactggct agataaaccg
     1621 ttacttttta gccatttact gtcgtgaact ttcagcgtct cgttcgccgc aaatcgcacc
     1681 aagcccgcac aatcctgctg ataccagcgt ggacttggcc cctggcggag ctgttcttgt
     1741 gcaatgcgta caaaccaggc gcgaaacagc ccggattgtt cgacattcag catttcgcta
     1801 tgggcaacaa cacaacacag ccagcaaatc agcgccagca gcccgtgtct catagcggct
     1861 gccaggttat gggtagccac tgccaggcga caccgggttc catctgggcc agcttcatga
     1921 cataacgcgg ttgttgagat aaagcgtcca gcttcggcat taataaagtt tgtgcggcgt
     1981 tataaaaaac cggttcgaga ttcttcggca gactggtcaa cgtttcgtta cgcagcagtt
     2041 tcgccacgcc ttgtggattg atatagagcg gaacgatgcc atcagtgggt attacatcca
     2101 ccattgccgg gcgggtttta ttcagtgttt gcagggcgtt attaaccagc gtgtcatcga
     2161 gggagaaaag cagcgttttg ttttgcatcg ccagcgacac acggaaaaaa taatccgaca
     2221 ttaattgatc gggttgcgcc gcctgcgctt tcggatactg accgtatcgg gaactcactt
     2281 cgcgacacca gatttgcgct tcgccctgct gagtctggct taccggcaaa acaccttctg
     2341 gcgctttgct ttcgtgcgca ccaatatttt gcgtaaacag tttccctggc aattgcgcct
     2401 gttcggcagt gccatcaaac tgaccgacaa acagcggggt ttgcaatttt gagtcttcat
     2461 accagcacag ccccgcggca ccgtctaacg ccccattcag cttgtcgttt tcctggctga
     2521 tgtgcgaaag catctcttcg gcaataccgt gtgaatacgg caccgccaca cagaagctgg
     2581 ctccggcagg catactgttc cagaccggcg taaaatcgaa actggcatct acgctggcgg
     2641 attcatcatt taacgccaca aaactgtgcc agccgtcgtt acccatttcg aagcgtacgc
     2701 cagcaaaaga aggcattaac cgctggtagc caaaacccag caacctggcg ctggccacga
     2761 tgcgcaggcg tactggcgtt ttttcggcag tacgctcttc caggccaaag ctggcttgcc
     2821 agcgtttttt accgctcaac aaatcacttg cgatcgccgt ggcttcggta tcctgctgat
     2881 catctttaaa caacatatcc gtactggaaa acaccagcat cttgtcctga tatgtcgcga
     2941 acatcagggc gttattgccg ttatagcgca actgataaac aggaatagtt tcactattta
     3001 tcttgatgct actgatttcc gttttgctta actggctgtc gctggtagcg gcaaacaaca
     3061 atggctccag cagtttgctt aaaccactgc gctggatcag caccatgtaa tgtgaaagat
     3121 ggccttgttt atcgtgccat agcgccgcct gcgcgggctg atctaagagt gacgaaaaga
     3181 gcttatcttt cagcgtgaga tcgtgttcat agacaatgcg gcgaatgctg ccttcaatgc
     3241 ccagacgatc ggcatgattc tgataataga aaacgaaatc ttcgctcaga acatcgtgga
     3301 gaaacggaat ggtgaggaga tctttgggaa gctggctcag agagtcgctg tctagaaaga
     3361 ggtccggctc attgagatcg atttgcagat tgttgtgcac caccagcggc gacaacgttt
     3421 tttctggccc actgccagca tattgcaacg cccagacgcc agcggaaagc agtgctattg
     3481 cgccaaaacc tacaagacca tagaaccgcc agcctttcgt ctttttttca ccactcattg
     3541 ccacattcct tgtgtatagc cagccatttt ttacgggcac agccaaactt taccgcgccc
     3601 taatacgaca aaagcccaga ctttgcagcc tggacttttc aattcaaaca agggagatag
     3661 ctcccttttg gcatgaagaa gtaaaattat tcttcttctg gctcgtcgtc aacgtccact
     3721 tccggagcga tttcatcatc cccttccgcg gcactgccgt cgatggtatc cagatcttcc
     3781 tcgtcaaccg gttcagcaac acgctgcaga cccactacgt tttcatcttc cgcagtacgg
     3841 atgaggatca cgccctgggt gttacggccc acgatgctga tttctgaaac gcgagtacgt
     3901 accagcgtac cggcatcggt gatcatcatg atctggtcgc agtcatctac ctgcaccgcg
     3961 ccaaccacta aaccgttacg ttcggtaacc ttgatggaga taaccccttt cgtcgcacgc
     4021 gacttggttg ggtattccgc cactgcagta cgtttaccgt aaccgttttg cgttgcggtg
     4081 aggattgcgc catcgccacg aggcacgatc agagagacga ctttatcgcc ttcacctaag
     4141 cgaataccgc gcacaccagt ggtattgcag cccatcgcac ggacagaaga ctctttaaag
     4201 cgcaccactt taccttcggc ggagaacagc attacttcat cttcgccact ggtcagatca
     4261 acgccgatca gctcatcgcc gtcaaccagt ttgatcgcca ctttaccggc ggtacgcaga
     4321 cggttgaatt cggtgaggac ggttttcttc acagtaccgt tagcggtcgc catgaagact
     4381 ttcacgcctt cttcaaactc ggtcaccggc aggatcgcgg tgatacgttc gtcctgttcc
     4441 agcggtagca ggttgacgat cggacgaccg cgcgcgccac gagtggcttc cggcaactgg
     4501 taaactttca tcgaatagac gcgaccacgg ctggagaagc acagaatatg gtcgtgcgtg
     4561 ttcgccacca gcagtcggtc gataaagtct tcttctttaa tacgtgcggc agatttacct
     4621 ttcccgccac gacgctgcgc ttcgtattca gaaagcggct gatatttaac gtagccctgg
     4681 tgagagagcg tcacaaccac atcttcctgg gtgatcagat cttccaggtt gatgtctgcg
     4741 ctgttggcgg tgatttcagt acgacgtttg tcaccgaact gttcacgaac cagctccagc
     4801 tcttcgcgga tcacttccat cagacgatcg gcgctaccaa gaatacgcaa cagttccgcg
     4861 atctgatcca gcagctcttt gtattcgtcg agcagttttt cgtgctcaag gccggtcagt
     4921 ttctgcaaac gcagatccag aatcgcctga gcttgctgtt cggtcaggta gtacagacca
     4981 tcacgcacgc cgaactctgg ctccagccat tccggacgcg cagcatcgtc gccagcacgt
     5041 tccaacatgg cggcaacgtt gcccagctgc cacggattag caaccagcgc agttttcgct
     5101 tctgcaggcg tcggcgcatg acggatcagt tcgatgatcg ggtcaatgtt cgccagcgcc
     5161 acggctaatg cttcaaggat atgagcacga tcgcgagctt tacgcagttc gaaaatagta
     5221 cgacgggtca ccacttcacg gcggtgacga acaaacgccg cgatgatgtc tttcaggttc
     5281 atgatcttcg gctgaccatg gtgcaatgcc accatgttga taccgaaaga aacctgcaac
     5341 tgggtctggg agtagaggtt gttgagcaca acttcaccga ccgcatcgcg tttcacttca
     5401 atcacgatgc gcataccgtc tttgtcagac tcgtcacgca gcgcgctgat gccttccacg
     5461 cgtttttctt ttaccagttc cgcaatcttc tcgatcaggc gcgctttgtt tacctgatac
     5521 ggaatttcgt ggacgataat ggtttcacgt ccggttttgg cgtcaacttc cacttcagca
     5581 cgggcgcgga tatatacctt gccgcgaccg gtacggtaag cttcttcaat accgcgacga
     5641 ccgttaatga ttgccgccgt cgggaagtcc ggccccggga tgtgttccat cagcccttca
     5701 atgctgatgt cttcatcatc gatatacgcc agacaaccgt tgatgacttc cgtcaggttg
     5761 tgcggcggga tgttggttgc catacctacg gcgataccgg aagaaccgtt caccagcagg
     5821 ttaggaattt tggttggcat gacgtcggga attttttccg tgccgtcata gttatcaacg
     5881 aaatcgaccg tctctttttc gagatcggcc atcagttcat gggcaatttt cgccagacgg
     5941 atttccgtat aacgcattgc cgccgcagag tcgccgtcga tggaaccgaa gttaccctga
     6001 ccgtctacca gcatgtaacg cagcgagaat ggctgcgcca tacggacgat cgtgtaataa
     6061 accgccgagt caccatgggg atggtattta ccgattacgt caccaacgac acgggcagat
     6121 tttttatagg ctttgttcca gtcattgcct agtacgttca tggcgtaaag tacgcgacgg
     6181 tgtaccggct tcaggccatc tcggacatct ggcagcgcac ggccaacaat gaccgacatc
     6241 gcataatcca gataggagct ctttagctct tcctcaatgt tgaccggtgt aatttctctc
     6301 gcaaggtcgc tcatctaacc gctatccctc tactgtatcc cggattcaaa ggtcgcaaat
     6361 tataacacag ccgcgcagtt tgaggtaaac ttatacgctt tattcacatc caatgcctga
     6421 tatactcgtt tgtcttgcca attacggagt agaagtgcca atgaatgccg aaaaatcgcc
     6481 ggaaaaccat aacgtagacc acgaagagat cgctaaattt gaagccgtcg cctcccgctg
     6541 gtgggatctt gaaggtgagt tcaaaccgtt gcaccgcatt aacccgctgc gtctgggcta
     6601 tattgccgag cgtgctggcg gtttatttgg caaaaaggtg ctcgatgtcg gttgtggcgg
     6661 cggcattctg gccgagagta tggcgcgcga aggcgcgacg gtgaccggtc tggatatggg
     6721 ctttgagcca ttgcaggtgg caaagctgca cgcgctggaa agcggcattc aggtggatta
     6781 cgtgcaggaa accgtggaag agcacgcggc aaaacatgcc gggcagtatg atgtggtgac
     6841 ctgcatggag atgctggagc acgtacccga tccgcagtca gtggtcagag cctgtgcgca
     6901 actggtgaaa ccaagcggcg atgtcttttt ctcgacgctt aaccgcaacg gcaagtcatg
     6961 gctgatggcg gtggttggcg cggaatatat tttgcgcatg gtgcccaaag gtacgcatga
     7021 tgtgaagaag tttattaaac cggcagagct gcttggctgg gtggatcaga ccagtttaaa
     7081 agagcgacat ataaccgggc tacattacaa cccgatcact aattccttta agctcggccc
     7141 cggcgtggat gtgaactata tgctgcacac gcagaataag tgaggttgat gtttggccgc
     7201 gccaatgcct gatgcgacgc ttgcagcgtc ttatcaggcc tacaaatgct ctccgtaggc
     7261 cagataaggc gtttacgccg catccggcaa ttgtgatggt cagacagcga ttaccatttc
     7321 accgtcatcg acaaaaaccc tgccgtctgg gcaaaatcat cactcccttt ctgccacgcc
     7381 acgctgccgc gcagggacac tcgctgactg atattgcccg tgactcccac ttttatttca
     7441 ccccgtagct tcaccgcatc gtcactgata gtgctgccat cttcttcaat ttccgtcgca
     7501 tggggatcgt gataataatt cagatctaat gttggtgtga caccaaccgc ggtacgccat
     7561 tcgctgtgta aacccagccg cgtctgaata tcatccccct gcgattgtga cacgcgcgca
     7621 tggttagcgg cggtaaaatc atcctgctgc acgccctgat aaatcacctg cgcctgcggt
     7681 tcaatcacca caccacgccc cggtaaccac tgatacccgg cttccagcga ggcgataatc
     7741 cccgacgaat ggtaacgatc cacgccatct tcatgttcgg aaacatcatt gttaaaccac
     7801 gcgtattgca gccagctatc cagccaggcc ccctgcttct gattaccgtg ctgataccag
     7861 cttgaggtca gcccaacggc ataaccgtgg ttctggttat cggcgcgagt tccggtcata
     7921 ttcgagcggc tgtcgccctg attatcactg tagccaccaa ccgccccaag catccactcg
     7981 ccatccgtgc cccagcgccc gctaaacaag ccgccgctaa gctgcaccgt agaagtgtct
     8041 tcctgctgag ccagttgccc tgccgccgtg taatgataac gtccgccgat aacacgtaaa
     8101 ttcagcgtct gaccatcgcc accagcgtga tcgcgtcgct ccatcataaa cgcctaattt
     8161 gccgcccgca ggttattgag ataaccgcca actttggcat tcagcaccgg ctggtaagca
     8221 ggcgtagggg ccgggtcggg ggttgggtca ggatccggcg tgggatcagg atcgggagtc
     8281 gggtctggct caggtggcga tggcggcgtt acttcttgcg atcgcagaca ccagtcgttg
     8341 ttatcttcca ctagcgtgta gtcatacgct cccatgttga cgtagccact gccagccaga
     8401 ctgaactgtg cattgttttg aaactgcgtg ggatcagctg cgaaatcaac cactttaatg
     8461 cctgtcgatg tcggctcacc aatccctgta atgaagttaa ccaccacagt tgtgttgcca
     8521 gcagtattac cgttcatcac caattgatcg cttaccgagt catcgccgtt taattcgctg
     8581 tcgaatagta gcgtaccgcc gccggtataa tcaccgttta ccgtcagcgt atcgccagcg
     8641 acgccatttt gcaggctaac cgcaccggac gtattggtca gatcaccatt aaccgtcacg
     8701 tcagcaagat aggtcaggct gtcagtttca aggttggtat taagctcgcc tgtcaacgtc
     8761 acgccatcgg tagcatttac cagcgtccca tcgccgttaa atagtgaggc attaagggcg
     8821 acggaagtat cctgcccggt cagacgcaaa accgtaccat cgctgatgtc gatagtgccg
     8881 ctggaagtag catcaattga ctgaatatcc tgatccgcgc cagtaacgaa cgttgcccca
     8941 tcaccaaccc acagtgacga agcataagga aagatatccg caacatcgcc tttcagggtg
     9001 ccttcttcga cacgtaccgc cgactgcgtt gtaccgctgg cggtcagctc cagcgtaccc
     9061 gcccccgttt tagtaaatgt gctaccctca tcctgatgct gcccgctgct gtcagccatc
     9121 agtgcgcccc actgcgtgtc tacccccgca tcaactgcca cttcaccgtc ggcgcgcatt
     9181 tcaatatcac gcccatgctc agtcgtcgta gagcgagtat cgacattggc agtaatctcc
     9241 atcacgcttt cttgttgctt atcggtaaag ataacctggc ggttatagtg ggtatctcca
     9301 agctgcgagt tgtcgctcac catcagcgta ccggaggcga tttgtgttgt gcccaaataa
     9361 ctattattgt ttgccagact tacctgcccg tcgctcgcat cgaccaccac gtagacgcca
     9421 tctttcccgt cgccgccgat atgcaaattg tcgccacctg caagatctac cacatcctga
     9481 ctgccgataa ccgttccgct gctgccactg acttcaaggc catcattgta tgatgtgccg
     9541 ctctgccagg tggagaaatc agagagatcg agcacaccgc cgtttaacac gatcgactgc
     9601 ggatcgtcct ggagagcggt aagatctgcc gcgtcgcctt ccagcgaaag caccgcacca
     9661 tcatcaacga ctatatcgcc ggttagcgcc atcgactgcg cccctgccag cacgtagctg
     9721 ccgttttgcg caatggtgag ctgaccagca ccttcgatga tgccagcaaa actgccctga
     9781 ttaacagtaa cgttgccacc agcatcgata tttaaagtgc cattctgaaa gcccgtcaat
     9841 gcgtgcacaa aagtttgttg ggtcgagcca acgtttagct cagcctgatt ctgatactga
     9901 tcaatactcc ctatcgtcag accgtagcag tcttgcggat cgtcctggca atgcgtatcg
     9961 ccgacattca tcagggagtt gctgcgaccc agggtaactt caccgttttc aatctgcatc
    10021 tcaccagtaa agtcattgtt atctgcatta agtaccagat cgccggaacc tgttttggtg
    10081 attaacccgg taccagcaat agagtcaaca gctccgtcat tctctgtatt gccgataacc
    10141 agcgtttttc cgtcggcaat atcaaaggta acttcactta agccgagata cataaagcca
    10201 cccgccgcag tggaaggacc atctccatag cctgctgcgc tattgttctc atcgactaac
    10261 acgccgccgt tctggctgta gctgtcatca acagaaatat caataagata gggagccgtc
    10321 gcgctattgg tatatatcgc cccgccataa ccttcggctg tgttatttgt aaaggcggtg
    10381 ttatttatta tcgtataacc tgaaggatgc ttgctgtcgc tattattatc ggtaacatcg
    10441 attgccccgc catcgccatc actgtaactt gttgatgtat atgcttggtt attgttaaaa
    10501 acatcatcac ttaaatagac gtcattatta atggtataaa ttgcgccacc tttgccatca
    10561 ttagcgatat tattgcgaaa catggcgtta gtgacacgta aatcgacggc accagtatca
    10621 ttagtaccag aagaatagat tgcgccacca tagccgcctg cgacgttacc ggaaaaaata
    10681 acatcagtaa gattcagcgt tgagttttct ttagcaaata tggccccgcc gttattatat
    10741 tctcctgtaa cggtgttatt ggcaaacaga gtcattccag tttcattttc tggtaatagt
    10801 gaaaattccg ctccttgttg caaaaatacg gccccaccgc tggcattatt ggtcatatcc
    10861 gaaaaaacca gccattgccc atcggc
//
LOCUS       DADLSE010000123        10859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLSE010000123 DADLSE010000000
VERSION     DADLSE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.123"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            83..424
                     /locus_tag="JN692_003162"
     CDS             83..424
                     /locus_tag="JN692_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7265399.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="JN692_003163"
     CDS             complement(415..1341)
                     /locus_tag="JN692_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7265400.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRGAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="JN692_003164"
     CDS             1431..2429
                     /locus_tag="JN692_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7265401.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIADGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMIVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="JN692_003165"
     CDS             complement(2426..2644)
                     /locus_tag="JN692_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265402.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN692_003166"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="JN692_003166"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7265403.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN692_003167"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="JN692_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7265404.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="JN692_003168"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="JN692_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7265405.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="JN692_003169"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="JN692_003169"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7265406.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="JN692_003170"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="JN692_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7265407.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="JN692_003171"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="JN692_003171"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7265408.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="JN692_003172"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="JN692_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7265409.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2648 a         2802 c         2683 g         2726 t
ORIGIN      
        1 tgcgatttgt aagattaatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gccccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct gacggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatagtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcagca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacttgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatctttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcaac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DADLSE010000124        10763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLSE010000124 DADLSE010000000
VERSION     DADLSE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.124"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            283..3150
                     /locus_tag="JN692_003173"
     CDS             283..3150
                     /locus_tag="JN692_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7265410.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN692_003174"
     CDS             complement(3250..5178)
                     /gene="dhaR"
                     /locus_tag="JN692_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7265411.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5397..6467
                     /gene="dhaK"
                     /locus_tag="JN692_003175"
     CDS             5397..6467
                     /gene="dhaK"
                     /locus_tag="JN692_003175"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7265412.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYITRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6478..7110
                     /gene="dhaL"
                     /locus_tag="JN692_003176"
     CDS             6478..7110
                     /gene="dhaL"
                     /locus_tag="JN692_003176"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7265413.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7121..8539
                     /gene="dhaM"
                     /locus_tag="JN692_003177"
     CDS             7121..8539
                     /gene="dhaM"
                     /locus_tag="JN692_003177"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7265414.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            8859..10556
                     /locus_tag="JN692_003178"
     CDS             8859..10556
                     /locus_tag="JN692_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7265415.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     VPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMENPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDRKKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT         2814 a         2704 c         2683 g         2562 t
ORIGIN      
        1 gctgtttgtt gtaatatttt tgccatattc atagtaacga attggaataa gtaacgacaa
       61 cttagggaat aactcgatat aaactcacct ttgtaagggg ggtgttaaat atatattaat
      121 tcctgtgttc ctgattttgc ccggtaatag agaattattc tcagagaagc tcatcatgat
      181 tgcaccatac agatatcatg atgataagag gatattattc attgacttaa acaaaatgac
      241 aaaatccata gacccatagc ctgggtacta tataggagtg gtatgggcat caaacaacac
      301 aatgggaata ccaaagccga tcgtctcgct gaattaaaaa tccgttcgcc ctcaattcaa
      361 ctgataaaat ttggcgctat tggtttgaat gcaattatct tttcccccct gctgatagct
      421 gctgatacag gaagtcaata tggcaccaat attactatta atgatggtga cagaattaca
      481 ggagataccg ccgatccatc aggaaacctc tatggtgtaa tgaccccagc aggaaacacg
      541 cctggcaata tcaacctggg taatgatgtc accgtcaatg tcaacgacgc ctctggatat
      601 gcaaaaggaa tcattattca gggcaaaaac agctccctga cagctaaccg actcacagta
      661 gatgttgttg gtcaaacctc tgccatcggc attaacttaa ttggtgacta tacccatgct
      721 gacttaggca caggcagcac cattaagagt aacgatgacg gcatcattat tgggcatagc
      781 tcaacactaa cagccactca attcaccatt gaaaactcga acggtatagg cctaaccatc
      841 aatgactatg gcaccagtgt cgatcttgga agcggaagta aaatcaagac cgatggaagt
      901 acaggtgttt atatcggtgg tctcaacggc aataacgcca atggtgctgc gcgttttacg
      961 gcgacagacc tgacaatcga tgttcagggc tacagcgcca tggggataaa cgtacagaaa
     1021 aactctgttg tcgatctcgg aacaaacagt accattaaaa ccaatggcga taatgctcac
     1081 ggcctctgga gctttggcca ggttagcgcg aatgcactca ctgttgatgt aactggagcc
     1141 gcggccaatg gcgtcgaagt tcgtggtggt acaaccacta tcggtgcaga tagccatatt
     1201 tcttccgcgc agggcggtgg cctcgtcacc agtggttcag acgcgacaat caatttttct
     1261 ggcacggcag cgcaacgaaa cagcatcttt tccggcggtt cttatggtgc ctcggcccag
     1321 acggcaacgg ctgttatcaa catgcaaaat accgatatta cggttgatcg taatggcagt
     1381 ctggcgctgg gtttgtgggc gctcagcggc ggtagaataa ccggagacag tttggctatc
     1441 accggcgcgg caggagccag aggtatttat gccatgacca acagccagat cgacctcacg
     1501 agcgatctgg tcattgatat gagtacaccc gaccagatgg ccatcgcaac gcaacatgac
     1561 gatggttatg ccgccagccg catcaacgcc tcgggtcgta tgcttatcaa cggtagcgtt
     1621 ctttccaaag gtgggctaat caatctggat atgcaccctg ggtcggtttg gacaggttcc
     1681 tccctcagcg ataatgtcaa tggcgggaaa ctggacgttg caatgaataa cagcgtctgg
     1741 aacgtaacaa gtaattctaa tctcgacacg ctggcgctga gccattcaac tgtcgatttt
     1801 gccagccacg ggtcaactgc cggcacattt accacattaa acgtagagaa cctgagcggt
     1861 aacagtacct ttattatgcg tgctgatgtt gttggcgagg gtaatggcgt taataataga
     1921 ggggatttat tgaatatcag cgggagtagt gctggtaatc acgtattggc tatccgcaac
     1981 cagggcagcg aggccacaac gggaaatgaa gttctgacag tggtaaaaac cactgacggc
     2041 gcggcctcgt tcagcgcgtc ttctcaggtt gagttggggg gatatctgta cgatgtgcgt
     2101 aaaaatggca ctaactggga gctttacgct tccgggacag ttccggaacc gactcctaat
     2161 cctgaaccca caccagctcc cgctcagcct cccatagtca accccgatcc tacgcctgaa
     2221 cccgctccca cgcctaaacc caccacgacc gcagatgctg gcggcaatta tctcaatgtc
     2281 ggttacttat tgaactatgt tgaaaaccgt acgctgatgc aacggatggg tgacttgcga
     2341 aatcagagta aagacggtaa tatctggttg cgcagttatg ggggaagctt ggactccttt
     2401 gccagtggca aactgagcgg ctttgacatg ggttacagcg gtatccagtt tggtggggat
     2461 aaacgtctct ctgatgtaat gccgttgtat gtcggtctgt atattggctc aacacatgca
     2521 tcgccggact atagcggagg cgacggtacc gcacgttcag actacatggg aatgtacgcc
     2581 agttacatgg cacaaaacgg tttttacagc gatctcgtta taaaagcatc gcgccagaaa
     2641 aatagtttcc acgtactgga cagtcagaac aacggcgtta acgccaacgg cactgcgaat
     2701 ggaatgagca tctccctgga agccgggcag aggttcaacc tgtcccctac tggttatggg
     2761 ttctatatag agccgcaaac ccagcttaca tacagccacc agaatgagat ggctatgaag
     2821 gcgagtaatg gcctcaatat acatctgaat cactacgaat cgctgctggg gcgtgccagc
     2881 atgatactgg ggtatgacat caccgcaggc aacagccagc tgaatgtcta tgtgaagact
     2941 ggcgctatcc gcgagttttc aggggatacc gaatatctgt tgaacgactc ccgggagaag
     3001 tacagtttca aaggtaatgg ctggaataac ggcgtgggag tcagtgcaca gtataacaaa
     3061 cagcacacat tctatctcga agcggattac acgcagggta acctctttga tcagaagcaa
     3121 gtcaacggag gatatcgctt cagcttttaa gtgcgggata gacaagccct taaacgatgc
     3181 tgaaaattgt tagcgtgcgc cggaatgaaa tacagatcac tctgatgtat cagaaatcgt
     3241 ttctgtcttt catacccggc gcttaaactg ccctgcatca atgccatgtt gcttcatttt
     3301 ccgccacagc gtagtgcggc cgatcccaag taaagccgac atttcctgaa tgcgaccgcc
     3361 tgtgacttgg gctgcgttaa taattgcctc tttttcaact tccgcaaatg acagactggt
     3421 ggaaaggcgg gtggcgctga catcatctgt cgcctgctcg gtaaacagat gttcaggtaa
     3481 atcactgacg cgaatgcgcc cgttatcact actcagagca agattctcga tgacgctgta
     3541 aagttcaaaa tcgttgcctg gccatgcaca agaaaccagg cgagcgaggg catcgtcatc
     3601 aattttcagc cgcgtagaga agcgtttttc aagactgcgt aatttgttat tcaccagcgc
     3661 cggaatgctg ccacgccgca tacgcagagg cgggatggta atttcaaatg catgcagcgc
     3721 gtaatacagc tggcgactaa aacgattttg ttccaccagc attgcgaggt ccgcggtcgt
     3781 tgttgcaatc actttgacat caattggtat taaacgccgc gcatccagtc gcgtgataac
     3841 cccctgcttg ataacctgaa gcaaagcaga ctgtaactcc accgccagat attcaatctt
     3901 ttcaagaaac agcgtgccgc cgtgtgccag ttccagccga ctcagacggc cattttcatt
     3961 gtccgtgcga tcgccaccaa taaattcttc cgccagcgca gcatcaccat ataactcaca
     4021 attgacggcg atataaggac ctgcagcacg ctcgctttca ttatgaattg cctgacttag
     4081 cagtgccttg cccacgccct cttctccaca aagcaggaca ggaaagctac tgcgcgccgc
     4141 ctggcgacca aaatgaatca agcggcgggt ttgcggatcg tcctgtggca tatgagcgaa
     4201 ggtatggctg acttttccta attgactggt cattaactgc cgcatctgtt ccacaggatg
     4261 gagcaacaaa ataaagctgg ttccctgcgt ttctattatc ggtttaaggg ttatcaccgc
     4321 atcaataaac tggtgctggc tttcaaaggt tgcttctacg tgtttgagcg gatgtgcctg
     4381 ttttattgct tgttgcaata cggcgggtaa cgttaagagt tcagtgattg cccgtccctg
     4441 acttgccgtc gcgtcaaggc gcaagacccg cgccgcctgg gcattaataa attgcaaatt
     4501 accctgctcg tcccagctaa tcacgccatc atccatactt tctaacaggg cattaagttg
     4561 atttaaatga cggttagttt cagcgagcaa actgtccgtc agcagtaaat taccgacctc
     4621 gcgggcgatt gccaacgtca acggcaaatc agctgcggta gtttgctcaa ccggacacgc
     4681 cagcgctatt gttcccgtca atcggccctt gctgtcaaac aacggcgttg cacaaaaggc
     4741 ccagttccag agtgcctgtt tgaaatgttg atcggccatc gttttcacgg cctgaccaga
     4801 gatagccgct aacgatagcg cacaagtacc aataattccc tcggcgcaat acgtgccgtc
     4861 attgaatccc agtgcactta gctgctgcaa ggtttgcgga tcgccattac ggctgagaat
     4921 gcaggcggtt tcatcgagga taaacagcgc acactctcgc ggtgccatat attcccaggc
     4981 atcttccagc gcagcctgcc cgagcgtcag catcgctttc ttacgccgat aaatagaagc
     5041 aaatgtcacg ccctgggcct gatgtggtac gttccatgtc tcccgtttca tcagcttatt
     5101 gcatcgctcc caggaggttg caattaaggg agatatgccc cgaccatcgt tgttaaaagc
     5161 gccactcata tcccgcatcc cttatgacaa aaggtgttcc ggctcgcaat tttaagtatt
     5221 aatacacgta aaagtttcaa tatggaacat tcattaaagc atatgtttca ctatgaaaca
     5281 taatttgggc taattttctt ttcccttgcc tgatgcacaa tggattcatc ggcagtcagt
     5341 ggtcgccgtg tcgttgaaca tcatccatgc cctaccgtaa ttgctggagc aaaataatga
     5401 aaaaattgat caatgatgtg caagacgtac tggacgaaca actggcagga ctggcgaaag
     5461 cgcatccatc gctgacactg catcaggatc cggtgtatat cacccgagct gatgcccctg
     5521 ttgcaggaaa agtcgccctg ctgtcgggtg gcggcagcgg acacgagccg atgcactgtg
     5581 gttatatcgg tcaggggatg ctttcggggg cctgtccggg cgaaattttc acctcaccga
     5641 cgcccgataa aatctttgaa tgctccatgc aaattgatgg cggcgaaggt gtactgttga
     5701 ttatcaaaaa ttacaccggc gatattctta actttgaaac agcgaccgag ttactgcacg
     5761 atagcggcgt aaaagtgacc actgtggtca ttgatgacga cgttgcggta aaagacagtc
     5821 tttataccgc cgggcgacgc ggcgttgcca acaccgtatt aattgaaaaa ctcgtaggcg
     5881 cagcggcaga gcgtggcgac tcactggacg cctgtgcgga actggggcgt aagttgaata
     5941 atcaaggcca ctcaataggt atcgctctcg gtgcctgtac cgttcctgcc gcgggcaaac
     6001 cttcttttac ccttgcggat aatgagatgg agtttggcgt cggcattcat ggtgagccag
     6061 gtattgaccg ccgccccttc tcttcccttg atcaaaccgt cgatgaaatg ttcgacaccc
     6121 tgctggaaca tggctcatac catcgcactt tgcgtttctg ggattatcaa caaggcagct
     6181 ggcaggaaga accacaaacc aaacaaccgc tccagtctgg cgatcgggtg attgcactgg
     6241 ttaacaatct tggcgcaact ccgctttctg agctgtacgg cgtctataac cgcctgacca
     6301 cacgttgcca gcaagcggga ttgactatcg aacgtaattt aattggcgcg tactgcacct
     6361 cactggatat gaccggtttc tcaatcacct tactgaaagt tgatgacgaa acgctggctc
     6421 tctgggacgc cccggtccac accccggccc ttaactgggg taaataagga gaaagcaatg
     6481 tcactgagca gaactcaaat tgttaactgg ctcactcgtt gtggcgatat tttcaacacc
     6541 gagagcgagt atcttaccgg gctggatcgc gagattggcg acgctgacca cgggctaaat
     6601 atgaaccgag gctttagcaa agtggtggaa aaactccctg ctatcgcaga taaagatatc
     6661 ggtttcattc tcaaaaatac cggtatgacg ctgctttcca gcgtcggtgg tgccagtggt
     6721 ccgctgttcg gtaccttctt tatccgcgcc gcacaggcga cccaggcacg gcaaagcctg
     6781 acactggaag agctttatca gatgttccgc gatggcgcgg acggcgtaat cagtcgcggg
     6841 aaagccgaac ctggcgataa aaccatgtgt gatgtgtggg tgccggtggt ggaatcgtta
     6901 cgtcagtcca gcgagcaaaa tctctctgtt ccggtggcgc tcgaagctgc cagtagcatc
     6961 gccgaatccg ctgcacaaag tacgattacg atgcaagccc gcaaaggccg cgccagttat
     7021 ctcggtgaac gcagtattgg tcaccaggat cccggcgcga cctcggtgat gtttatgatg
     7081 caaatgttgg cgttagccgc aaaagagtaa ggaattggtg atggtaaacc tggtcatagt
     7141 ttcacatagc agccgactgg gagaaggtgt cggtgaatta gcccgtcaga tgttaatgag
     7201 tgatagttgt aaaatcgcca ttgccgcggg aattgacgat ccacaaaatc ccattggtac
     7261 cgatgccgtc aaagtgatgg aggccatcga atctgttgct gatgccgacc atgtgctggt
     7321 catgatggat atgggtagcg cattattgag tgctgaaact gcgctggaat tgctggctcc
     7381 cgagatcgcc gcaaaagtac gtttgtgtgc tgcgccgttg gtcgaaggta cactggcagc
     7441 aacggtcagc gcggcctcgg gggcggatat cgacaaagtt atctttgacg ctatgcatgc
     7501 gctggaagcc aaacgtgaac aactgggttt accgtcctcc gacactgaaa tctctgacac
     7561 atgtcctccg tacgatgaag aagcccgttc tctggcggtg gtcataaaaa accgtaacgg
     7621 cctgcatgta cgtccggcct cccggctggt ttatacctta tcgacattta atgccgatat
     7681 gttgctggaa aaaaacggca aatgcgtcac accagagagt attaaccaga ttgcgttact
     7741 acaagttcgc tataacgata cgctgcgcct gattgcgaaa gggccagaag ctgaagaggc
     7801 actgatcgct ttccgtcagc tggctgaaga taactttggt gaaacggagg aagtcgctcc
     7861 acctactctg cgtcccgttc tgcctgtttc gggtaaagcc ttttattatc aaccagtttt
     7921 atgtacggta caggcaaaat caaccctgac cgcggaagaa gaacaagatc gattacgcca
     7981 ggctattgac ttcacgttat tagatctgat gacgttaaca gcgaaagcag aagccagcgg
     8041 gcttgacgat attgccgcaa tcttttctgg tcaccataca ctgttagatg atccggaact
     8101 gctggcggcg gcaagcgaac tccttcagca tgaacattgc acggcagaat atgcctggca
     8161 gcaagttctt aaagaactta gccagcaata ccagcaactg gatgatgaat atctacaagc
     8221 tcgctatatt gatgtggacg atcttctgca tcgcaccctg gtccacctga cccaaacgaa
     8281 agaagaactc ccgcagttta actcgccaac tattctactg gcggagaaca tttatccttc
     8341 cacagtactg caactggatc cggcggttgt aaaaggtatc tgccttagcg ccggaagtcc
     8401 ggtatcccac agcgccctaa tcgcccgtga actggggatt ggctggattt gccagcaggg
     8461 tgagaaactg tatgcgatac aaccagaaga aacgctaacg ctggacgtta aaacgcaacg
     8521 tttcaaccgt cagggttaag ccaccgttcg gatggcatcg ttctgatgtc atccggcaat
     8581 ttacccagca ctgatgtttt ttaactacct gttatatttt ttaccaattt atgatgattt
     8641 ttcttcgcta atcgcgaacc aaatgtcgct ggctggctaa taaacagacg ttatttgatc
     8701 actctatttc gccgttattt atccctttaa ttcccttttc taaaatgcct gaccgttcgc
     8761 agaatgagat ttcgatcatg cagctagtgc gatcctgaac taaggttttc tgatacttga
     8821 ataccgtttt attcggtttc gccaaaggag aatgattgat gaaatccccc gcaccttctc
     8881 gcccgcaaaa aatggcgtta attccagcct gtatcttttt gtgtttcgct gcgctatcgg
     8941 tgcaggcaga agaaacaccg gtaacaccac agccgcctga tattttatta gtgccgctgt
     9001 ttaatgatgt gcaaaacgcc aaactttttc cggaccaaaa aacctttgcc gatgccgtgc
     9061 cgaacagcga tccgctgatg atccttgctg attatcggat gcagcaaaac cagagcggat
     9121 ttgatctgcg ccatttcgtt aacgtcaatt tcaccctgcc gaaagaaggc gagaaatatg
     9181 ttccgccaga ggggcagtca ctgcgcgaac atattgacgg actttggccg gtattaacgc
     9241 gttctaccga aaacaccgaa aaatgggatt ctctgttacc gctgccggaa ccttatgtcg
     9301 tgccgggcgg acgttttcgc gaggtatatt actgggacag ttacttcacc atgttaggac
     9361 ttgccgaaag cggtcactgg gataaagtcg cggatatggt ggccaatttt gctcatgaaa
     9421 tagacactta cggtcatatt cccaacggca accgcagtta ctatttaagc cgctcgcaac
     9481 cgcccttctt tgccctgatg gtagagttac tggcgcagca tgaaggcgat gccgcgttga
     9541 agcaatacct gccgcaaatg caaaaagaat atgcttactg gatggacggt gttgaaaacc
     9601 tgcaagccgg acaacaggaa aaacgcgttg taaaacttca ggatggtacc cttctcaacc
     9661 gctactggga cgatcgcgat acgccacgac cagagtcatg ggtggaagat attgccaccg
     9721 ccaaaagcaa tccgaatcga cctgccactg aaatttaccg cgacctgcgc tctgccgctg
     9781 cgtctggctg ggatttcagc tcgcgctgga tggagaaccc gcagcagtta aataccttac
     9841 gcaccaccag catcgtaccg gtcgatctga acagcctgat gtttaaaatg gaaaaaatcc
     9901 tcgcccgcgc cagcaaagct gcgggagata acgcgatggc aaaccagtac gaaacgctgg
     9961 cgaatgcccg tcaaaaaggg atcgaaaaat acctgtggaa cgatcaacaa ggctggtatg
    10021 ccgattacga cctgaaaagt cataaagtgc gcaatcagtt aaccgcggcc gccctgttcc
    10081 cgctgtacgt caatgcggca gcgaaagatc gcgccaacaa aatggcgacg gcgacgaaaa
    10141 cacatctgct gcaacccggc ggcctgaaca ccacgtcggt gaaaagtggg caacaatggg
    10201 atgcgccaaa tggctgggca ccgttacagt gggtcgcgac agaaggatta caaaactacg
    10261 ggcaaaaaga ggtggcgatg gacattagct ggcacttcct gaccaatgtt cagcacacct
    10321 atgaccggaa gaaaaagctg gtggaaaaat atgatgtcag caccaccgga acggggggcg
    10381 gcggtggcga atatccatta caggatggct ttggctggac caatggcgtg acgctgaaaa
    10441 tgctggattt gatctgcccg aaagagcaac cgtgtgacaa tgttccggcg acgcgtccga
    10501 ccgttaagtc agcaacgacg caaccctcaa ccaaagaggc acaacccaca ccttaaccag
    10561 cacttactcc gacaacgtca ttcggcttct ccggatggcg ttgtcggcag ataacaccat
    10621 atctatggtt ttttctacac agacggtttt tcaagtcctg gcgggaagta ttgcgcaaat
    10681 gtttgccata cgtggttgcc gaagatagcg tcatttctac gggtaagtgt ttcactttca
    10741 tcacccggcg atttacgccg acg
//
LOCUS       DADLSE010000125        10713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLSE010000125 DADLSE010000000
VERSION     DADLSE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.125"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            429..1547
                     /locus_tag="JN692_003179"
     CDS             429..1547
                     /locus_tag="JN692_003179"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7265416.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWNIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="JN692_003180"
     CDS             1613..1861
                     /locus_tag="JN692_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7265417.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="JN692_003181"
     CDS             1926..2294
                     /locus_tag="JN692_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7265418.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKPDQQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="JN692_003182"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="JN692_003182"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7265419.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDATILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /locus_tag="JN692_003183"
     CDS             3149..4396
                     /locus_tag="JN692_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7265420.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKDTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN692_003184"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="JN692_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7265421.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCISFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN692_003185"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="JN692_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HAY7265422.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN692_003186"
     CDS             complement(9096..10319)
                     /gene="cusB"
                     /locus_tag="JN692_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HAY7265423.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFEQAEPHAEKMPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRHLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPNKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN692_003187"
     CDS             complement(10335..10667)
                     /gene="cusF"
                     /locus_tag="JN692_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HAY7265424.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2596 a         2959 c         2607 g         2551 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgcaccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatcgccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgcctcctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tggaatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag cccgatcaac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccacaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 agacaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccagc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggagatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccaccag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctcg gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac agcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatctccgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcagcgagac tgatgccata ctgcgccagg cgctgagggt cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt attcggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattt tcgcgaccac gttatctttg gcgatattca ttcccgcgcg cagatcaaac
     9661 gcggtgatca cgccatcaat gggcgctttg agcgtaaagc gagtctggat tttttgcgtg
     9721 gcgatcaggt ggcgaatatc cgcctccggc attcccgcca gtcgcagccg ctcaagaatg
     9781 ccttcagtct gggtcgccgt accgccggtt tcgcgcagca gtaaatactc actctgcgct
     9841 tccacccagt cgggaatggt caggtcgaga agcggtgtgc ccttttgtac tttatcgccc
     9901 acggtaagcg gatacacctt gtcgataaac ccggcagcgc gagcctgcac aatggcatac
     9961 tgatactcgt tgtaactgac attcgccggg aaactctggg caaaagtcag cggcccgcgc
    10021 gtgacggtag ccgttttcac ccccagattc tgcgtctgag tcgggtcaat gcgcacacca
    10081 gacgcagaac tctcttcatc ggcatatttc ggcaccagat ccatatccat aaacggcgat
    10141 ttacctggtt tatcgaaccg cgtattggga tacatcgggt cgtaccagaa taagatttta
    10201 cgttctgcgg tcggcatttt ttctgcgtgc ggttccgcct gttcaaacca ggtaaaacct
    10261 gccgctgaaa taataccgcc cgcgatcatg ctgccgataa taagcgcgat ttttttcatc
    10321 tcattaaacc tgggttactg gctgacttta atatcctgta ataaagaaag gttgccctgc
    10381 tggacaaaat taaacgccac tttgtcgccg gttttaattt cactcatttt cgtctgcggg
    10441 gtgatggtaa agcgcatggt catctccggc cagttgacgg cagcaatcgg atcgtgatgg
    10501 atggtgattt ttttgctttc cagatcaaca ccttttacca caccggtggc actaatgacc
    10561 tgtggttgtg cttcgctcat ggtttcatga tgatgttcgt tagcctgggc attaaagcca
    10621 ataacggtaa acagactgaa cattgcgact tgcagtgctt ttttcattta ttctctcctg
    10681 gagttaaaaa ttattgctgc caaccgcccc cta
//
LOCUS       DADLSE010000126        10548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLSE010000126 DADLSE010000000
VERSION     DADLSE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.126"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(75..1103)
                     /locus_tag="JN692_003188"
     CDS             complement(75..1103)
                     /locus_tag="JN692_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7265425.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGVRVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPATLDDIPVTWVSTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1278..2870
                     /locus_tag="JN692_003189"
     CDS             1278..2870
                     /locus_tag="JN692_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7265426.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVASAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2880..4052
                     /gene="malY"
                     /locus_tag="JN692_003190"
     CDS             2880..4052
                     /gene="malY"
                     /locus_tag="JN692_003190"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7265427.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4156..5157
                     /gene="add"
                     /locus_tag="JN692_003191"
     CDS             4156..5157
                     /gene="add"
                     /locus_tag="JN692_003191"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7265428.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5193..6233)
                     /locus_tag="JN692_003192"
     CDS             complement(5193..6233)
                     /locus_tag="JN692_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7265429.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6476..6601
                     /gene="blr"
                     /locus_tag="JN692_003193"
     CDS             6476..6601
                     /gene="blr"
                     /locus_tag="JN692_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7265430.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6874..7089
                     /gene="ydgT"
                     /locus_tag="JN692_003194"
     CDS             6874..7089
                     /gene="ydgT"
                     /locus_tag="JN692_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7265431.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7175..7615
                     /locus_tag="JN692_003195"
     CDS             7175..7615
                     /locus_tag="JN692_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7265432.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7692..8273
                     /gene="rsxA"
                     /locus_tag="JN692_003196"
     CDS             7692..8273
                     /gene="rsxA"
                     /locus_tag="JN692_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7265433.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8273..8851
                     /gene="rsxB"
                     /locus_tag="JN692_003197"
     CDS             8273..8851
                     /gene="rsxB"
                     /locus_tag="JN692_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7265434.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLQIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDESNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8844..>10548
                     /gene="rsxC"
                     /locus_tag="JN692_003198"
     CDS             8844..>10548
                     /gene="rsxC"
                     /locus_tag="JN692_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7265435.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNDTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KA"
BASE COUNT         2418 a         2732 c         2684 g         2714 t
ORIGIN      
        1 ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac caagtatagc
       61 agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag attgcgtgaa
      121 tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtgat accaagttcc
      181 cgcgctggcg tgctgaccca ggtaacaggt atatcatcaa gtgtggctgg tgtcgcatcg
      241 gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct ttccccgctt
      301 tgccgccctg ctttcagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa
      361 accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc
      421 ttctggctgg aagtgcactc caacacccaa tcgctgtgaa acggcaggcc aaattttagt
      481 agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc
      541 agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc
      601 tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa
      661 atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctgct tcctgcagcc
      721 ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc
      781 tgctcgccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct
      841 tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa
      901 ccaatgacgc cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc
      961 agctcttcaa tggcggcgtt aacgcgtact cctgtggcgg tagagattcg ccctttgcca
     1021 ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg
     1081 gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa
     1141 cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgact
     1201 cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct
     1261 ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct
     1321 tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta
     1381 tgctcggcat tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg
     1441 gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta
     1501 gtttcctgcc tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag
     1561 gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct
     1621 ggttgaccaa taaaggcatt ctgccaacca cagatgccgc cgttctgaaa gccaataaca
     1681 tccagagcat tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg
     1741 gtattatcgt ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg
     1801 cattcttcgg cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg
     1861 gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc ttgggccata
     1921 tgataaacag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt
     1981 tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac gcaggcggca
     2041 cgcaggaagt ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga
     2101 gttgcccgac cactcacggt ttttctgaaa gcgtcacgcg tttcctttcg caaggtaaaa
     2161 tgcctgcgtt tctcggcggt ctgccaggtg cagcgttagc tatgtatcac tgcgcgcgcc
     2221 cggaaaatcg ccataaaatt aaaggtctgc tgatttctgg cctgatcgcc tgcgtcgttg
     2281 gcggcactac cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca
     2341 tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg
     2401 gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa
     2461 ccaagtggta catggtgcca gtggtggcgg caatctggtt tgtcgtttac tacgtcatct
     2521 tccgtttcgc tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagttgcca
     2581 gctcaatcga aaaagccgtt gccagtgcgc cgggtaaatc aggttacaac gttcctgcaa
     2641 tcctcgaagc attaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc
     2701 tgcgtttgtc tgtggaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg
     2761 caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc
     2821 agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata
     2881 tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag tgggattatg
     2941 tcgctgaccg tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg
     3001 ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct
     3061 acagccgctg gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc
     3121 attacaccgc aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt
     3181 cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct
     3241 atgacgcatt ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag
     3301 agaagcaggc tgacggctgg ttttgcgata tgggcaagct ggaagccgtt ttggcgaaac
     3361 cagaatgtaa aattatgctc ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt
     3421 gcgatgagct ggagatcatg gctgacctgt gcgagcgtca tgatgtgcgg gttatttccg
     3481 atgaaatcca tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg
     3541 cgcggggaga ctgggcgttg ctgacgtcgg gatcgaaaag tttcaatatt cccgcgctga
     3601 ccggtgctta cgggattata gaaaatagca gcagccgtga tgcctattta tcggcactga
     3661 aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct
     3721 atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt
     3781 atatctcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca
     3841 cttatctggc ctggcttgat ctacgtccgt tgaatattga cgacaacgcg ttgcaaaaag
     3901 cgcttatcga gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc
     3961 gtggttttgt ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg
     4021 gattaataaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt
     4081 gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatatccag
     4141 aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt
     4201 gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt
     4261 cctgcacaat cccttgaaac actgattccc cacgttcagg tcattgccaa cgaacccgat
     4321 ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc
     4381 tgccgccgcg tggcatttga aaacattgaa gatgcagccc gtaacggcct gcactatgtc
     4441 gagctgcgtt tttcaccagg ctacatggca atggcacata agctgcctgt agcgggtgtt
     4501 gtcgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag
     4561 cttatcggca ttatgagccg aaccttcggc gaagccgcct gtcagcaaga gctggaggcc
     4621 tttttagccc accgtgacca gattaccgca cttgatttag ctggtgatga acttggattc
     4681 ccgggaagtc tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc
     4741 gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt
     4801 gcggagcgta ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc
     4861 gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta
     4921 gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt
     4981 aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg
     5041 ccagctgctg ggttatcccg tgagcaaatc cgccaggcac agattaatgg tctggaaatg
     5101 gctttcctca gcgcagagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa
     5161 aaatggatgg tgcaaattgc accatccatt tttcacgcaa ggcacaacgt cgcgcgatgt
     5221 ttggcggatt cgatgcccag ctcaatcaac tccattacct ggattgcctg gcttgccgga
     5281 accggatttt caccatcgcc atttaacgca tcacgaatcg ccgcatagta agccggataa
     5341 ttcccaggca ccgtcaacag cgtttcttcg acacgttcct caccttccac gcgggtaagt
     5401 acgccatcac gcatatcgta gccccagtct tcctgcggca gacgctcgcc atttttcaga
     5461 cgttcttcct gtggatcgag gccatatttc acataactgc ctcgggatcc atgcacgata
     5521 taccgtgctg actcagcggc tgccagcatg gtaccgtgta aaatgactcg ccgctgtgga
     5581 taggacaaga tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa ctgtgccaaa
     5641 tctaccgtca tgctgaccgg taaaccaaat agcgtaatgg cctgatcaag aagatgcggt
     5701 gctaaatcgt accagatacc gctgcctggg ccgccctgtt cacgccaacg atcgcgcacc
     5761 tgcggacgga agcggtcaaa atgagactca aagtaagcaa cttcacccag cacgccttcc
     5821 gcgagcaaac cttttagcgt caagaaatcg ctatcccaac gacggttatg gaatacagac
     5881 agcacacgcc ccaggctttt tgccagcgca tccagctctc gcgcttgtga cagtgtcacg
     5941 gtaaagggtt tatcaacgac cacatgttta cccgcctcaa gcgccgcttt ggctaacggg
     6001 aaatgggtat cgttgggtgt aggaatgaca atcaggtcta tgttgggatc gttaaacaga
     6061 tgcttcggct cagagacaac cgtaaccgtt ggccagtcgg cttttacttt tgtttcatca
     6121 ctgctggaga ttaccgccag ttccagcccg ggcgtgcccg caatcagggg cgcatggaag
     6181 gttttgctcg cataaccata cccaatcaac ccaacacgga tgttgtcgct catgtcattg
     6241 cctctcattg aagtatgatg gctatttgac actatccttt acccacgctc aacagtttaa
     6301 taacctgcca gcaataaggg atgttgttta acttaagtca aaaaaatagc gaattttcca
     6361 acgacaaaag ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc tattattgct
     6421 aagtaacatt taccccctga agttaatgga tcaatcaaga gagatgtggg ctgtaatgaa
     6481 tcgtcttatt gaattaacag gttggatcgt tcttgtcgtt tcagtcattc ttcttggcgt
     6541 ggcgagtcac attgacaact atcagccacc tgaacagagt gcttcggtac aacacaagta
     6601 agctctgcac ttgtggagcg acatgctgcc cgtccgggtg catgttttca cttgtcggat
     6661 attaaaccag gaatttatta tcttgttcga tgttgttggt gattgtcagg gatagtaaag
     6721 cgcgttactc ttttcagagc agtttttcat ccctgaatat ttgccatcag cgcattctta
     6781 ttattgtaat aacacaagcg ttcacttttg gtgaaatttt atcatcacta ataataccta
     6841 cccctttggt attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa
     6901 tttcgcaaaa tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc
     6961 ctgacggacg atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag
     7021 ctggtttctg gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat
     7081 gtccaataaa gaaagtagcg atcggttaca aaagccttat aataacgccc ctaaaattag
     7141 atttgcctgt ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat
     7201 tggaggctgg ttgcttggcc cgttggcctg gcttttagtc gctctgttga gtacgacgct
     7261 ggcgttgttg ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg
     7321 acaagcactg acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg
     7381 gtattacaca ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca
     7441 ctatattatc tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc
     7501 cgtggaagtt ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat
     7561 cgtcccctat ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc
     7621 ttacagttaa cctgttgtcg cctgctctgg attaacagat aataggcggc ttttttattt
     7681 caggccgaaa aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact
     7741 ttgtactggt caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa
     7801 ccgcgatggg catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct
     7861 ggcttatcga tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat
     7921 ttattctggt gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc
     7981 cggtgcttta ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc
     8041 tcggcgtggc gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg
     8101 gtttttccgc cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgagaac
     8161 gccttgctgt ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg
     8221 caggtcttat gtctctggcc tttatgggct ttagtggttt ggtgaagttg taatgaatgc
     8281 tatctggatt gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg
     8341 ttatgcctcc cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat
     8401 cttaccgcag agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc
     8461 catcagctgt aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct
     8521 acaaattgcc gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct
     8581 aacgcctgcg cggatggtgg cggttattga tgaaagtaac tgtattggct gcactaaatg
     8641 tattcaggct tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat
     8701 gagtgatctc tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc
     8761 gttgcaaccg gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt
     8821 gcgtatcatt cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa
     8881 tctgggattt caacggcggc atccatccac cggagatgaa aacccagtcc aacgataccc
     8941 ccctgcgcca ggtaccactg gcgcagcgtt ttgttattcc actgaaacag catattggcg
     9001 ctgaaggtga gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc
     9061 gtggtcgcgg caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg
     9121 cgccccactc tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg
     9181 atggtgaaga ctgctggatc ccgcgcgacg gctgggtcga ttatcgctct cgcagtcgcg
     9241 aagagttaat cgagcgcata catcagtttg gtgttgccgg gctgggcggt gccggattcc
     9301 cgacaggcgt taaattgcag ggtggtggag ataagattga aacgttgatt atcaacgctg
     9361 ctgagtgcga gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg
     9421 tcgtagaggg tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca
     9481 ttgaagataa caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg
     9541 atatttctct gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct
     9601 acattctgac cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa
     9661 tgcaaaacgt cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta
     9721 ccgagcgtgt tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac
     9781 ggctggggac gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc
     9841 aaatggtgat tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg
     9901 tcgtaaagat taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg
     9961 aagaacaaag ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgtg gatcttttgc
    10021 cgcaacagtt gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca
    10081 ttgccgactg cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccttgg
    10141 tgcaatattt ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg
    10201 ccgcagaagc caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg
    10261 ctcgccttga acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga
    10321 ttgctgccgc tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga
    10381 ttaaagcggg cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag
    10441 cgcaagccag agcgaaacag gcagaactgg agcaaactaa cgacgcagca accgttgctg
    10501 aaccacgtaa aactgccgtt gaagcagcta tcgcccgctc caaagcgc
//
LOCUS       DADLSE010000127        10495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLSE010000127 DADLSE010000000
VERSION     DADLSE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.127"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JN692_003199"
     CDS             49..579
                     /locus_tag="JN692_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7265436.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN692_003200"
     CDS             597..1235
                     /locus_tag="JN692_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7265437.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDADKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN692_003201"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN692_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7265438.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN692_003202"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN692_003202"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7265439.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN692_003203"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN692_003203"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7265440.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN692_003204"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN692_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7265441.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN692_003205"
     CDS             complement(5552..6613)
                     /locus_tag="JN692_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7265442.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN692_003206"
     CDS             complement(6610..8007)
                     /locus_tag="JN692_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7265443.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLGQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN692_003207"
     CDS             8163..9161
                     /locus_tag="JN692_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7265444.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN692_003208"
     CDS             complement(9272..10177)
                     /gene="ompG"
                     /locus_tag="JN692_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7265445.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(10222..>10495)
                     /locus_tag="JN692_003209"
     CDS             complement(10222..>10495)
                     /locus_tag="JN692_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HAY7265446.1"
                     /translation="TQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFML
                     YTTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR"
BASE COUNT         2641 a         2811 c         2486 g         2557 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tgccgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagcgtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgccccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc acgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac cacgttccac gtttacccgc gctataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgttctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttcattat tgttccttgt attatcctgg
    10201 gggaatgcct tccccctgta tttagcgaat tgctatttcc gtttctgcat caaagaaatg
    10261 acatttcgtc atatcaaaat gaatagtgat attttctcct gcatgataat catttaacgc
    10321 accagcacgg accactaact cgtgcccccc aaccgtggtg tagagcataa attccgcacc
    10381 ggttaattct gccacgctaa ttttggcgga aatgttattt tcctcttgcg cgtccggatg
    10441 aatatcttcc gggcgtattc ccatcacgat gggcttatgc aaactttcct gtgtt
//
LOCUS       DADLSE010000128        10467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLSE010000128 DADLSE010000000
VERSION     DADLSE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.128"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(43..402)
                     /locus_tag="JN692_003210"
     CDS             complement(43..402)
                     /locus_tag="JN692_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7265447.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(399..674)
                     /locus_tag="JN692_003211"
     CDS             complement(399..674)
                     /locus_tag="JN692_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7265448.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(733..1857)
                     /locus_tag="JN692_003212"
     CDS             complement(733..1857)
                     /locus_tag="JN692_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7265449.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN692_003213"
     CDS             complement(1857..4592)
                     /gene="rbbA"
                     /locus_tag="JN692_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7265450.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(4589..5656)
                     /locus_tag="JN692_003214"
     CDS             complement(4589..5656)
                     /locus_tag="JN692_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7265451.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(6022..7644)
                     /locus_tag="JN692_003215"
     CDS             complement(6022..7644)
                     /locus_tag="JN692_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7265452.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPSA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(7906..9495)
                     /locus_tag="JN692_003216"
     CDS             complement(7906..9495)
                     /locus_tag="JN692_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7265453.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            9992..10467
                     /locus_tag="JN692_003217"
                     /pseudo
     CDS             9992..10467
                     /locus_tag="JN692_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
BASE COUNT         2703 a         2587 c         2316 g         2861 t
ORIGIN      
        1 cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat gattcaaacg
       61 ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg cagccgcatt
      121 attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa tcttttctgt
      181 acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc gcagggaaag
      241 ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat aacctgacaa
      301 gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa cagctggttg
      361 cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac ccctatactt
      421 agtaaccagt cacgcacgct ttctacagcg cctttatcgg tatctggcga cggatgaggc
      481 cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc tcttccttcc
      541 ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt gatcccttga
      601 ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg taaagcctgt
      661 accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag tatgaagtca
      721 agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca gtgcgatggt
      781 gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga tttcgaatcc
      841 ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg gcatggtcag
      901 cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac cggaaagcat
      961 ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg aacgcgctat
     1021 cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca cgcccagcat
     1081 aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca tcagcaccag
     1141 cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg ccatcatgat
     1201 ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt cacgctcgcg
     1261 gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga tgatcgccat
     1321 caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg tttccagcga
     1381 taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt caccgttgat
     1441 aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg catcgacgtt
     1501 cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag gcggaatatt
     1561 tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg cggtgatcat
     1621 ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg ataactgcga
     1681 ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg taacggtcgc
     1741 tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg ctttatcacc
     1801 gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat ggcgcatcat
     1861 ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat ggctatcagc
     1921 aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa cgttccgcgg
     1981 gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc aggcccttcc
     2041 agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa tgtgataatc
     2101 gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag cagccccatt
     2161 ccagtggcaa tgatgatata cagcagcgcc gccagggtga gcgtcaggaa actgccttta
     2221 tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt cagcatcccc
     2281 agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt gggggtcacg
     2341 taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc gcttagcatt
     2401 gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg caggcttttt
     2461 acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg gctggcgggt
     2521 gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg cacgtaacct
     2581 tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag ttccacaggc
     2641 gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac cgtgatatcg
     2701 cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg ttcgataaag
     2761 taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg gtcgcggtcg
     2821 agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat tatcagcatc
     2881 aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg cagttccagc
     2941 gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg cggcgcgtgg
     3001 gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc cgcttcctgc
     3061 aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac cagttcctgc
     3121 ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc gcaacgttcc
     3181 gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg cgagagatcg
     3241 accatcaact gccagaacat atccctcgcc accggatcga caccagaagt aggctcatcg
     3301 aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg ctggcgaatg
     3361 ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa acgctcgctc
     3421 atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg ggcatgtaac
     3481 tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga catatagccc
     3541 acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag ccacgcctca
     3601 ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt accgcagccg
     3661 ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac gtgatcaacg
     3721 gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat ctctgcgttt
     3781 tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc ttgcggtaac
     3841 agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag ctcttcggcg
     3901 ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa gcgttcggcc
     3961 tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg gcgaatactg
     4021 tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt tggctcatca
     4081 aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt ttgcttcatc
     4141 ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt gctggtcagc
     4201 agctcattaa ttcgcacttc ccgctccgct ttatcgtgac cgaacaggcg agcgaaaaaa
     4261 tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc ctgcggcatc
     4321 caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc gccacccagc
     4381 accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa taaactcgac
     4441 ttcccgacgc catccggacc aatcagcccg accatgcaac gggccggaat atcgagagtg
     4501 atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag ttgcgcgaca
     4561 ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg aggtcgtcag
     4621 gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg gttttgacat
     4681 attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg aacatcagtt
     4741 tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca ctggcgacaa
     4801 aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc cgggcttcac
     4861 cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata tagacgtcgc
     4921 tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct ggctcggcaa
     4981 cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg atatctgcgg
     5041 caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga atgatattgg
     5101 tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct gattccagcg
     5161 cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca gaaatagccc
     5221 ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacggagtcc agttcggcct
     5281 ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc tccagcaaag
     5341 cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct tccagtcgct
     5401 gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga acaaactggc
     5461 cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata tccacttccg
     5521 tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc gggcgcaaca
     5581 gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac gccagatggc
     5641 gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac gcgtgttaca
     5701 actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga aacgacaaat
     5761 acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa cgggtagtaa
     5821 tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata agtgtgatct
     5881 ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcgtttcacg cctgttcagt
     5941 tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga tagtaacatt
     6001 aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa tcaaaggcat
     6061 aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta tctctggccc
     6121 tgcactaggg ttaatttggc aattatgcat gctgaacatt cttccagcat gtttactgtc
     6181 acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc caccatttcc
     6241 gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata gtgcttcaag
     6301 tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac aagagatagc
     6361 ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat taataatttc
     6421 cgctaattcg accggatcga gagcattggt catttcatca atagagacag ttatcggtcc
     6481 tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg ccattatttt
     6541 gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg tatcctcatc
     6601 aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg tttcatgatt
     6661 tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca aatcgatgat
     6721 aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa gaaacgtaca
     6781 tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta aaacattatc
     6841 tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct cattaaagtt
     6901 ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc taaggaaatt
     6961 ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca aaactgcacc
     7021 gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat caacaggtga
     7081 acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt cgaatttaat
     7141 atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg actcagtaac
     7201 tgcaacatca ttagcattat catttactgt catattatta agtaaataaa caatgctgca
     7261 ccaattaata tcggtattat caagagggca accgtaattt ttactattaa ttgtcacaat
     7321 aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata gcatactata
     7381 tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa tagattgttg
     7441 gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca ttgtcctggc
     7501 aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt ctttagtgaa
     7561 gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac tgccattagt
     7621 gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga ggaatacata
     7681 gttaacattt tatcttatga catacacctc atattcgatg ggaaaataat tatattttgc
     7741 acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca gagaaagtcc
     7801 ctaaagattg tagaggcgtc atcagtacat acctctacaa tcgattaaga aaacctatga
     7861 aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt gaggttgaaa
     7921 acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta tcttccagga
     7981 atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta atccataatg
     8041 gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat cttcaggttt
     8101 tggtctgaaa gcagtagtgt tattaaaata attttcatat acaggtgtgg gtgctatagc
     8161 attgcttcta attagatgga gaagatgatc atccatgtgt tgtttatgtt ttttattaaa
     8221 tatatttgct aattcctcag gatcaattgg gtctttcatc tgattaaatg gaacctgcaa
     8281 agcaccgaga caataacatt gattttcttc atttcttatt atcccgtgat gactggttaa
     8341 aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt tatacgtatc
     8401 accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa aattgatttc
     8461 atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat taccctccat
     8521 atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag tactaaatct
     8581 atcgcctgta tgatccccaa gggggataca aggggttatc gcatcgtcct ttaaaaaaac
     8641 atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt agagcaattt
     8701 attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata attcacataa
     8761 taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg aataaagtac
     8821 agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt gtgggttaat
     8881 cacaccacat tgtttataaa agtctatttt agaaaacaca gatttaacat agctatcaaa
     8941 cttatcctta ggaaacgtct ctttagagac atcccaacgc cagtcagctt taaaattacc
     9001 gctaagaaga gtaaagaaat tttcaggtat gatagaaagc ccattaactt tataaattat
     9061 gtctgattta tctccggaga aattaataaa ataataacca tccttacact caatcttaac
     9121 tgtaatggga gacgataatt ttatcacatg gttattgtct ggtataattg taaagataaa
     9181 cgtgttagta gtattatcgg caaaactatt atattcttga atatctatag atggctcact
     9241 attgtaaaac catattttct ccatctgaag actatgttca tttaccctcc agtgaccatg
     9301 ttgcaggaaa tagatgatat gcatgccaaa atcgttatct atttgcgaat cattatccag
     9361 tttatcaatt tcaactatct ttgaacgatg tgttttatca accttgctat ggtgttgatg
     9421 attattaact ttatctgaat gcttatcttt atggtttgaa gggatgtact taaatatatt
     9481 gtcaattgct ttcataaata acccgcgata tttttgacct gttagtaaca aataaatgat
     9541 acatgtctga aagacatatt tacgacgata taaaacaata atacttatat gttcgctgat
     9601 tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc atccctggaa
     9661 gacaattgta aaaaaaaaca acagaataag aattaatctt aggataaata tttatttatc
     9721 atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta tagttatgct
     9781 atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt atcattaatg
     9841 cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata tatcgggtag
     9901 cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg tggctttgtg
     9961 ctggaaagtt cagggcaacg tcgcgggtca tgtgctgatt tctctggcgg ccatatgcct
    10021 agcattattc actaccgcat ttattatcat ttcgcagctc acgcgcggcg ttaatacgtt
    10081 ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca tgatatgggg
    10141 ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg gccatgttat
    10201 ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt ccggtcactt
    10261 tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg tacaggctta
    10321 ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc tgctcggttt
    10381 catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg tcgccttggg
    10441 ttaaccgcaa tctgtgcctg tctaatt
//
LOCUS       DADLSE010000129        10460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLSE010000129 DADLSE010000000
VERSION     DADLSE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.129"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            93..647
                     /locus_tag="JN692_003218"
     CDS             93..647
                     /locus_tag="JN692_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7265454.1"
                     /translation="MAKLTDKQELFAREYLKDLNATQAAIRAGYSEDSAAVQGCKNLI
                     KANIAKRIAELKAERNEEVGIDAAYVLRRLVEIDQMDVLDILLQNGELKPIKDWPKVW
                     RTTLSGMDVVEMVSADSAALLKKIKWPDKVKNLELLGRHVSVQAFKDNVKNEVTGADG
                     GPVRTEITKLTPEQAAEVYRKMMG"
     gene            650..2269
                     /locus_tag="JN692_003219"
                     /pseudo
     CDS             650..2269
                     /locus_tag="JN692_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
     gene            2269..3810
                     /locus_tag="JN692_003220"
     CDS             2269..3810
                     /locus_tag="JN692_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HAY7265455.1"
                     /translation="MWPFKRKEPSAPAVPDKQPEPQPPEINDEVIASVRQKPRREFVR
                     YEPPPGVIPAPVRDAVLAMDATPYDTLNSHYPDFVYGGFPGYPYLALQAQLPEYRRMV
                     SVLAEEMTRKWIKVKAVGEGDDSHASRIAQLTDALEHYNVQDVFRLAIEHDGFFGRGQ
                     IYIDVRSPSGISAWTDPAELGSGLFISDKKIPKGSLLGLRVIEPVWTYPGIYNADNPL
                     SDDFYRPSEWYVMGKTVHASRMIDLISRPVPDMLKPAYNFGGLSLVQIAEPYVDNWLR
                     TRDSVGDMLHSFSLSGIKTDMTQVLSGKRDPNYAKRAELFNRTRDNRGLLMLDKTNEE
                     FFQFNTPLSGLDTLQAQAQEHMFFVSAIPSVKFAGLSPTGLNASSEGEIRVFYDTIAA
                     LATRLLKKPLKKVLDIIQLSEFGDIDPDITFEFEPLHELTREQLANIRKTEAETDQIY
                     ESTGAVTNNEVRERLATAQDSPYSGIDLSGDIEIDDTEIDNTEENPPQGPNADPEADF
                     TQRGD"
     gene            3851..4540
                     /locus_tag="JN692_003221"
     CDS             3851..4540
                     /locus_tag="JN692_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000552018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7265456.1"
                     /translation="MHNSVLYWLRAEYRKTDLAQDASPVNLMRGAMQQLARRWQKNFD
                     EMALRLARRFAGDVLKNSDASLYTALRDAGFTVPFRMTTEMNTALQASITENVNLIRS
                     IPQQHLPQVETLVMQSVGRGRDLKTLTDELEKRYGITRRRAALIARDQNNKATSVMQA
                     ARQRSVGITEGVWRHSRAGKTWRPSHVKANGKRFDLRKGMFLDGKWVLPGEEINCRCG
                     WEAVIPGLEKK"
     gene            4537..5886
                     /locus_tag="JN692_003222"
     CDS             4537..5886
                     /locus_tag="JN692_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7265457.1"
                     /translation="MIITEMLAFDRASVRQFDKVGRFQIERSNLSKANVCGYFGYEIP
                     GAEALGLDPKKLYQLYRDPDELRKAVSTFNNIPVLCRHKPDYPGAPAREYRVGTTHAN
                     SEFDGTYLVNGMSIWDNSAIAGIETDEQREISSSYAYVADMTPGTTPDGEPYDGVMRN
                     IVGNHVALVGDGRVGSDCLVMDSLPQELKRMKLSKKEVAVLTALGTYLAPRLAQDAAP
                     GDLLRLMAQYKRPAAIASAVKTAYSERLVQDMDIEPVELAQLMESAEAVPELVGDDDT
                     GLTDESKAFDTDSPMESVLALLSGKVPDDVLEKIKSALSPTTDEAPETKEADVKPDDV
                     KVDKPAMDAAIRLATDQATKRAAENFRAVRVAEAEVRPLIGDVVAMDCAEDVYRTALE
                     QTGIDIQGIHPGAYRSMVKFAVEQKQTAKSSPRVAMDQASASTFAADFPGAKLKRGY"
     gene            5888..6370
                     /locus_tag="JN692_003223"
     CDS             5888..6370
                     /locus_tag="JN692_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265458.1"
                     /translation="MNTFQTHMNQYPAPGIPVAFASANPHASYVAGDGGLITGPDGLV
                     IARFAWVTKGVAANKGAGAPAGFVPRDGQASIVEWLAGDSNTIYPGRECTLMVSGDFW
                     ALTTTTATIGQKVFASLATGEIATGAAGTSMAGFVETWFSVASAAAAKEVIKISTWSK
                     "
     gene            6370..7398
                     /locus_tag="JN692_003224"
     CDS             6370..7398
                     /locus_tag="JN692_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7265459.1"
                     /translation="MNKFKQHYATASRDYGIILPGAQAYLPKEYAADYGLAMDAQPAL
                     VTAANSGIPAYFTNYVEPELIRVLVTPMKAAQILGETKKGDWTTLSAQFPIAESDGEV
                     SSYGDYSNNGVVSANVNWVPRQSYHFQTFTVWGERELEMYGAARIGWAAELNVASALT
                     LNKFQNKSYFYGIAGLTNYGLLNDPSLSAPITPDTVDGKLKWDDKDGQGVYDDIVKLF
                     KQLVKQTNGHIERTDKMKLCMSPLAEVNLTKTNQYRVNVSDLLAKNFPAMTIETAVEY
                     TSDAGELVQLIAERLGEQDTGYCAFTEKMRAHAVVADTSSWKQKKSAGTWGAIIRQPL
                     AYAQMLGV"
     gene            7402..7749
                     /locus_tag="JN692_003225"
     CDS             7402..7749
                     /locus_tag="JN692_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265460.1"
                     /translation="MAEMVTVGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQ
                     VDKVFFDAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPG
                     VNRNDEVMGKPQE"
     gene            7755..8213
                     /locus_tag="JN692_003226"
     CDS             7755..8213
                     /locus_tag="JN692_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7265461.1"
                     /translation="MGTVTFDCRMFVSLYPEFSMVTPELLTAVFNQATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGVDGK"
     gene            8203..8787
                     /locus_tag="JN692_003227"
     CDS             8203..8787
                     /locus_tag="JN692_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265462.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPP
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            8784..9149
                     /locus_tag="JN692_003228"
     CDS             8784..9149
                     /locus_tag="JN692_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265463.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRVQKQAVTQADLRHLDNLNQQGAFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVPEIWPDWTRVIVCLQV"
     gene            9134..9679
                     /locus_tag="JN692_003229"
     CDS             9134..9679
                     /locus_tag="JN692_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265464.1"
                     /translation="MPSGVTLSVTESALYQALGDYLRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            9660..>10460
                     /locus_tag="JN692_003230"
     CDS             9660..>10460
                     /locus_tag="JN692_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7265465.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTLMLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTTQ
                     NAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAAS
                     QQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTE"
BASE COUNT         2536 a         2548 c         3034 g         2342 t
ORIGIN      
        1 agcctatcta cgggtgggct tgataatggc ttatacccta cacgggataa cttaactgat
       61 atccctttta acggataaac ggagcacaaa taatggcaaa gctcaccgac aaacaagagc
      121 tgtttgcccg tgagtacctg aaagatctca atgccacgca agcagccatc agggcgggtt
      181 acagtgagga ttctgccgcc gtacaaggat gcaaaaacct aataaaagct aatatcgcaa
      241 agcgcattgc tgagcttaaa gccgagcgaa acgaagaggt aggtatagat gccgcttatg
      301 ttcttcgtag attggttgaa atcgaccaga tggatgtgct cgacattctc ctgcaaaacg
      361 gtgagctaaa gcccattaaa gactggccta aggtatggcg cacaacgcta tcaggaatgg
      421 atgtcgtgga gatggtatcc gcagatagcg ccgcacttct gaagaaaatc aaatggcctg
      481 ataaggttaa aaaccttgag ttgcttgggc gccatgtttc tgttcaggcg tttaaagaca
      541 acgtcaaaaa tgaagtgact ggcgctgacg gaggaccagt cagaacagaa attaccaaat
      601 taacgcctga gcaggccgca gaggtgtata gaaaaatgat gggctaagta tgccgttacc
      661 attccccttc gattttaaac atcctgatta ccagatggtt tttgaatggc ggatggaacg
      721 cctacagcgc attcgccaga accctgaaat attgcccgta ttgaagcagt tttaccgaac
      781 caatccggct cagttcatca tcgactgggg catgacaacg gacccgcgta atattgatta
      841 tggcctgccg gtgaccattc cgtttttact cttccctaag caggaggagt ggatccactg
      901 gattatggaa cgctggagca atcgggagaa tggtattacc gaaaaatccc gtgaaatggg
      961 gctcagttgg accgcgatcg gactggcctg ctcgctttgt ctcttcaaca aagaaatggt
     1021 tatcggtttc ggctcccgta aagaggaata cgtcgacagc accggtgacc cgaaagcatt
     1081 gttctggaag gcgcgcaagt tcgtggaaac tctacctgta gagtttcgcg gttcgtggag
     1141 cgagaagaag cacgcgccat atatgcgtgt tgagtttcct gaaactggcg cggttatcaa
     1201 aggtgaggct ggcgataata tcggtcgtgg tgaccgtacg accctttatt ttgtggatga
     1261 ggctgctttt ctccagcgac cattacttat tgatgccgcg ctttcccaga caacccgatg
     1321 tcgtatcgat ctctcatcgg ttaacggcat gaacaacccc ttcgcacaga agcggcacag
     1381 tggaaaaatc ccggtgttta cgtttcactg gcgtagcgat ccgcgtaaag acgatgagtg
     1441 gtatcacaag gagtgcgaga aaattgataa cccagtcatc gttgcctagg aactggatct
     1501 taattaccag gcatcagcag aaggcatcct gatcccctca gaatgggtac aggctgcggt
     1561 tgatgcacat atcagactgg gcattcagcc cggcggccaa cggcttggtg caatggatgt
     1621 cgccgacgaa gggcgggata aaaacgcctg ttcgctccgt tatggcatcc tgctgaatga
     1681 cgtacaggag tggtcaggta aggggagtga catctatgac tctgtggtta aggtttttgg
     1741 tctgtgcgat gactttggtg ccgatgagtt tcgctttgat gaggacggat tgggtgctgg
     1801 cgttcgtggt gatgcgcgcg ctatcaacga actgcgtgaa gcggagggca tctgtcaaat
     1861 caccgccact ccattccggg gcagcgggag cgtgtttcat cctgaaaatg aagctgttcc
     1921 cggtgataat ggtaaaccgg ctcgtctgaa taaggacttt tttgtcaatg ccaaagccca
     1981 ggggtggtgg catcttcgca aattattccg caatacattt cgtgcactac agggtatgga
     2041 gtatgacccg gatgagatta tttccatcag cagcacaatg gaaaataaag accggttgtt
     2101 gatggagctg tcacaaccta cctggtcaaa aaatgccacc ggaaaaattc ttgtggataa
     2161 gcaacctgac ggaacgaaat ctccaaacct ggctgattct atgatgattg cttatgcccc
     2221 gatggaaatg cccatcgtaa tttctgatga ctttatggag tggatctgat gtggccgttt
     2281 aaacgaaaag aaccgtcagc gccggcggta ccggacaaac aacctgaacc acagccaccg
     2341 gaaattaacg atgaggtgat cgcttccgtc cggcagaaac cacggaggga gtttgttcgc
     2401 tatgagccac caccgggcgt tatcccggca ccggttcgcg atgctgtgct ggcaatggat
     2461 gcaacaccgt atgacaccct gaacagtcat tatcctgatt ttgtttatgg tggtttcccg
     2521 ggctatccgt atctggcact tcaggcgcag ttaccggagt accggcgtat ggtcagtgtg
     2581 cttgccgagg agatgacccg taagtggata aaggttaagg cggtcggaga aggggacgac
     2641 agccacgcgt cacgcatagc gcagcttact gacgcactgg agcactataa cgtacaggac
     2701 gttttcagac tggcgattga gcacgacggc tttttcgggc gagggcagat ttatattgat
     2761 gtgcgttcgc catcgggtat atctgcctgg actgacccgg cggagctggg atccggactg
     2821 tttatttccg acaaaaaaat cccgaaaggt tccctgctgg ggcttcgtgt tattgaaccc
     2881 gtctggacgt atccgggtat atataacgcg gataatccgc tgagtgatga tttttaccgt
     2941 ccgtccgaat ggtacgtaat gggaaaaacg gttcacgcca gccgcatgat tgatctgatc
     3001 tcccgtccgg ttccggacat gctgaagccg gcctacaact ttggcggcct gtcactggtt
     3061 cagattgctg agccttatgt tgataactgg ctgcgaacac gcgacagcgt gggcgatatg
     3121 ctgcattcgt tttcgctgag cgggattaag acggatatga cacaggtatt aagtggtaaa
     3181 agggacccga attacgcaaa acgcgcggag ttgtttaacc gtacccgtga taaccgcggg
     3241 ctgctgatgc tggacaagac gaacgaagag tttttccagt tcaacactcc cctgagcggc
     3301 ctcgacaccc ttcaggcgca ggcacaggaa cacatgttct ttgtcagtgc cataccgtcc
     3361 gtaaaattcg ccgggctgag cccaacggga ctgaatgcgt cgagtgaggg tgaaatccgg
     3421 gtgttttacg acaccattgc tgcacttgcc acccgtcttc tgaagaaacc gctgaaaaag
     3481 gtgctggata ttatccagtt gtctgagttc ggcgatatcg atcctgatat cacgtttgaa
     3541 tttgaacccc tgcatgagct gacgcgagag caactggcga atatccgtaa aactgaggcg
     3601 gaaacggatc agatttatga gagcaccgga gcagtgacca ataacgaagt acgcgaacgg
     3661 ctggcgactg cgcaggacag cccgtacagc ggtattgacc tgagcgggga catcgaaatt
     3721 gacgacactg aaattgacaa caccgaagaa aatccgccgc aaggcccgaa tgcagaccct
     3781 gaggcggatt tcacccaacg cggggattga ggcctggtac cgcagacaac tggataatgc
     3841 cgtcagtggg atgcacaaca gtgtacttta ctggctgcgg gctgagtacc gtaaaacaga
     3901 cctcgcgcag gatgcgtccc ccgttaacct gatgcgtggt gccatgcagc aacttgcccg
     3961 gcgctggcag aaaaattttg acgaaatggc cctgcggctg gcgaggcggt ttgccggtga
     4021 tgtcctaaaa aacagcgatg cgtcactgta cactgcgctc cgtgatgccg ggtttacggt
     4081 tcctttccgt atgacaacgg agatgaatac cgcacttcag gccagcatca cggagaatgt
     4141 gaacctcatt cgctccatcc cgcagcaaca tctcccccag gtggaaacac tggtcatgca
     4201 gtctgttggc cggggacgtg acctgaaaac gctgaccgat gaactggaaa aacgttacgg
     4261 catcacacga cggcgcgcgg cgctgattgc ccgcgaccag aacaataagg caacgtcggt
     4321 aatgcaggcc gccagacagc gttcggtggg tatcactgaa ggtgtctggc ggcattcccg
     4381 cgcgggtaaa acatggcgtc cgtcgcatgt gaaggcgaac ggtaaacggt ttgacctgcg
     4441 aaaggggatg tttctggatg gtaagtgggt actgccgggc gaagaaatca actgccggtg
     4501 tggctgggag gccgttattc ccggactgga gaaaaaatga ttattaccga aatgctggcg
     4561 tttgaccggg catcggtaag gcagttcgat aaagtcggtc gcttccagat tgagcgcagt
     4621 aatctcagca aggcgaacgt ctgcggttat ttcgggtatg aaataccggg ggcggaagcg
     4681 ctgggactcg accccaaaaa actttatcag ctttaccgtg accccgatga actgcgcaag
     4741 gcagtttcaa ccttcaacaa tattcccgtc ctgtgccgac acaaacccga ttatccgggc
     4801 gcgcccgcgc gcgagtaccg ggtggggacg actcatgcca acagcgagtt tgacggtacc
     4861 tatctggtta acggcatgtc catctgggac aactccgcca tcgcggggat agaaacggat
     4921 gaacaacggg aaatctcatc gtcatatgcc tatgtggcag atatgacgcc gggaaccacc
     4981 cccgacggtg agccgtatga cggcgttatg cggaatatcg tgggaaatca tgtggccctg
     5041 gtcggcgatg gccgggtagg gtcggactgt cttgttatgg actctctccc tcaggagcta
     5101 aaacgcatga aactgagtaa aaaagaagtg gcggtgctta ccgcgctggg aacctatctt
     5161 gcgccgcgtc tggcgcagga tgcggctccc ggggatttgt tacgcctgat ggcgcagtat
     5221 aagcgtccgg cagctatcgc cagcgcggta aaaactgctt acagcgaacg gctggtacag
     5281 gatatggata ttgaaccggt ggagctggcg caactgatgg aatcagcaga agccgtaccg
     5341 gagctggtcg gggatgatga taccgggtta actgacgagt cgaaggcatt tgataccgac
     5401 agcccgatgg aaagtgtact ggcgttgctg tccggcaaag ttcctgatga tgtgctggaa
     5461 aaaattaaat ccgcactgtc tccgacaacc gacgaagccc ccgaaacaaa agaggctgat
     5521 gtgaaacccg acgatgtgaa agtcgataaa cctgcgatgg atgcagcaat caggctggca
     5581 actgaccagg caacgaaacg ggctgctgaa aatttccgcg ccgtccgtgt ggctgaagct
     5641 gaagtgcgac cgctgattgg cgatgtggtg gcgatggact gcgccgaaga tgtttaccgt
     5701 accgcgctgg aacagacggg gatcgatatt cagggtattc accccggtgc gtaccgcagc
     5761 atggtgaagt ttgccgttga gcagaagcag acggccaaat cctctccccg tgtggcgatg
     5821 gatcaggcca gcgcatcgac gtttgcggca gatttccccg gtgcaaaact gaaacgaggt
     5881 tactgatatg aatacttttc agacacacat gaaccagtac ccggcaccgg gtattccggt
     5941 ggcgtttgcc agtgctaacc ctcatgcctc gtatgtggcg ggagatggcg ggctgattac
     6001 cggccctgac gggctggtaa ttgcccggtt tgcctgggta accaaaggtg ttgccgctaa
     6061 taagggggcc ggcgcaccgg cgggttttgt tcctcgtgac gggcaggctt ccattgtgga
     6121 atggctggcc ggggactcga acaccattta tccggggcgt gaatgcaccc tgatggtgtc
     6181 gggggatttc tgggcgctga ccaccactac tgcgacgatc gggcagaaag tatttgcctc
     6241 actggccacc ggggagatag ccacgggggc ggctggcacc tcgatggcgg ggtttgtcga
     6301 aacctggttt tccgttgcca gcgctgcggc ggcaaaagaa gtcattaaga tcagtacctg
     6361 gagcaaataa tgaataaatt taaacagcat tatgcgacgg caagtcgcga ctacgggatt
     6421 attcttcccg gagcacaggc ttatttgccg aaggagtacg ctgccgatta cggactggcg
     6481 atggacgcgc agcctgcgct ggtcacagcg gctaacagcg gtatccctgc gtatttcacc
     6541 aattacgttg agccagaact gatccgcgtg ctggtgacgc cgatgaaagc cgctcagatt
     6601 ctgggcgaaa ccaaaaaagg tgactggacg acactgtcgg cacaattccc gattgcagaa
     6661 tctgacgggg aggtgagttc ctacggggat tacagcaaca acggggtggt aagcgcaaac
     6721 gtcaactggg taccgcgcca gagctatcac ttccagacct tcaccgtatg gggcgaacgt
     6781 gagctggaga tgtacggcgc agcccgtatt ggctgggcgg cagagctgaa cgtggcgtca
     6841 gcactgacgc tgaataagtt ccagaataag tcctatttct acggtattgc cggactgacg
     6901 aactacggtt tgctgaatga tccgtcgtta tctgcaccaa tcaccccgga taccgttgac
     6961 ggtaaactga agtgggacga caaggacgga cagggcgtgt atgacgatat cgtgaaactc
     7021 tttaagcaac tggtgaaaca gactaacggt catattgagc gtaccgacaa aatgaaactg
     7081 tgcatgtcgc cgctggcaga ggtgaacctc accaaaacaa accagtacag ggttaacgtg
     7141 tccgatctgc tggcgaaaaa tttcccggcg atgaccattg aaacggcggt ggaatacacc
     7201 tctgacgctg gcgagctggt acagcttatc gcggagcgtc tgggtgaaca ggatacaggc
     7261 tattgcgctt tcactgaaaa aatgcgcgcc catgcggtag tggcagatac gtcttcctgg
     7321 aaacagaaaa aatccgctgg tacctggggg gcgattattc gccagccgct ggcgtatgca
     7381 caaatgctgg gggtgtgagt tatggctgaa atggtaacag tgggctgcaa actgccgaac
     7441 ggtctggtgc tggaagtggg tcagaaacgg gttcaggtgg caggctggcg gaataacgcg
     7501 gttaaaattg tggggggcta tggtctgacg caggtcgata aggtgttttt tgacgcctgg
     7561 ctggcggagc acggacagca accttatgtg aaaaatggcg ttatttttgc gcaggataag
     7621 gcgaacagtg cagtctcgca ggctacggag cagaaagccg ttaaatccgg ccttgaaccg
     7681 ctgccgcaga aaaatccggc tccgggcgtt aaccgcaatg atgaagtgat gggtaaacct
     7741 caggagtaaa cgctatgggt acggtgacgt ttgactgtcg gatgtttgtc agtctgtacc
     7801 cggagttttc catggtgaca ccggagctac tgacggcggt gtttaaccag gcgaccgcgt
     7861 tgtacctgga taacacggac gacagtccgg ttaccgacct gaaggagcgg gaacagcttc
     7921 tgttcctgct ggttgcgcat ctgtgcgcat tgcgggggct gggaaacggg agagacgggc
     7981 aggctggtct ggtggggcgt atcaccagtg cgtcggaagg ttctgtatcg gtatcggtgg
     8041 ataatagcgg cagtaatgat gcgtcgtggt ggtatctcca gaccccctgg ggtgctgact
     8101 actggcaggc gacagcgcca taccgctcaa tgcagtatca ttccgggggg gcaccttcgc
     8161 gatatccggg gcattattac cggggatacg ggaggggcgt cgatggtaaa taaagtcaca
     8221 ggtggcaaac agttccggca gaagctgaaa caggttgccg cgaacctttc attgggtaaa
     8281 aaactcaaag tgggttttct tgagggagca acttaccctg acggtacgtc agtggcttat
     8341 attgccgcag ttcaggagtt tggtggaaga gcggtcatac ccgcccgtga gcagacgctc
     8401 cactttcgct ataacgaaaa aacgggagaa accgggcacc gctttgtcaa agccggtaaa
     8461 gggaattttg ttcaggatgt ggttattcct gagcacacgg tcaccattcc cccccgtccg
     8521 ttcttccgta agatgatcga acataaaagt cccgaatggg gcgaaaaaat ggcgacctta
     8581 ttacgggcga atgattttga tactgcgacc gctctggtgt gtatgggaga acatatcaaa
     8641 gggcagttgc agatgtctgt tcgcgactgg gaaagtccgc ccaacgctgc atccactgcc
     8701 cggcaaaagg gttttaacaa cccgcttatt gaaaccggac atatgatgga cagcgttgat
     8761 tattctgtag acgggggcaa aaaatgaact ttcacggtat tgtttccggc gcagtacgtc
     8821 gggtaaatcc ctttacagac gcgctggttt atcgttcccg cgggagtaca cagcaggcag
     8881 attattcccg cgtacctgag tatgaagatc cggtttccgt cagggtgcaa aagcaggctg
     8941 tcacccaggc tgatttacgt catcttgata atcttaacca gcagggagct ttcgccacat
     9001 tgtataccga tggtaactgg tgcgggctta accgtgcccg gcaacaggga ggcgataaat
     9061 ttgttattgg cgatgaaacg tggctggtga ttgaggtacc ggaaatctgg cctgactgga
     9121 cgagggttat tgtatgcctt caggtgtgac cctctccgtt acggaaagcg ctctttatca
     9181 ggccctcggt gattatctcc gggggctttt ttctgatgct gggattgaac gaacgcagca
     9241 gaaccaggtc ccgatgcctc aggggaattt catcaccatg acgggcattg atgttaccgg
     9301 attatccact gcggtagtgg catattctgc gccagcgcag gctggtgagg gctctcagca
     9361 tatcacccgt accacaaaat ggcgttgcca gcttgatttc tacggccctc acgcggcaga
     9421 taacgcgcag gtgctggcaa cgcttttccg gtctgaattt tccgtacggc ttttccggca
     9481 gggcggtggg ctgatttccc cgctgtattg ctcagagccc tttaacacca cattccccaa
     9541 cggtcagcaa cagtatgaac cgcgctggac gcttgatatt cagatgcaga ttaaccctgt
     9601 ggtcacaact tccctgatgt tttttgacaa cgtgatcacc cggacaacgg aggctgataa
     9661 tgccaattcc actcagtaaa gatgtacaga taaatcccgg tgtgctggct gtggcgggta
     9721 atgccgttga tcttaacggc ctgctgttga ccagtaatcc gttacttccg gtcggcactg
     9781 tggttccgtt ttcctctccg gatgatgtgg ccgcgtattt tggtgcatta tccgatgagt
     9841 acgcacgcgc tcagctttat ttccagggct tcaaaaatgc caccaggacg ccggggcaat
     9901 tgttgttttc ccgtttcagt cctgccccat cggcggcctg gttacgtagc ggttcgttta
     9961 agggcgcgac tgttgaacag ctacaaaaac tttccggcac gctgatgctg agtattaacg
    10021 ggaaaagcgt cagcattgaa gtgaatttta acggtgtcgc cagcttcgcc gctgctgcaa
    10081 cggcactaca gacagcgctg accgcagcag tggctacagt ggtattcgat accacacaga
    10141 atgcttttgt cattactgtc gccggggcga aacccgggag taccacgata acgttcggca
    10201 gtggatcggc agcagaactc ctgaagatga ccagtaacac cggtgcggtg atatcccggg
    10261 gcgcgcctgt atctgatgtc cctgacacaa tggcagccat taaggccgct tcccagcaat
    10321 gggcgggttt ttccacggta tctgaggtca ctgacgagca acacctggcg ttttctgcat
    10381 gggcaaacgg gcagggcaag cgttactttt atgtggcatg gacaaccagc ggcaacgcca
    10441 gagtaaaagg cagtactgaa
//
LOCUS       DADLSE010000130        10415 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLSE010000130 DADLSE010000000
VERSION     DADLSE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.130"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="JN692_003231"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="JN692_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7265466.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="JN692_003232"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="JN692_003232"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7265467.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPPFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="JN692_003233"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="JN692_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7265468.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGVGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="JN692_003234"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="JN692_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7265469.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="JN692_003235"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="JN692_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="JN692_003236"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="JN692_003236"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7265470.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="JN692_003237"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="JN692_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7265471.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9370)
                     /locus_tag="JN692_003238"
                     /pseudo
     CDS             complement(8007..9370)
                     /locus_tag="JN692_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9393..10085)
                     /locus_tag="JN692_003239"
     CDS             complement(9393..10085)
                     /locus_tag="JN692_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7265472.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLMAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2553 a         2515 c         2753 g         2594 t
ORIGIN      
        1 aagggggggg ctttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctact aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg gtgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgctg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgttgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccactgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtac tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag cgggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgccagacgc ctcctttctt cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaagct gcggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccaaggtgta cctcctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa tgtctatccg ggccgtgtga
     2701 tggcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct
     3001 ttaaaagcga caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 caggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgta ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgct gatgaacagc
     3721 aggctgtgag tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt attgatcctt gatgagccta cccgtggagt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagtatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cttcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatag acttgtcggt aggctctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcac gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgtccaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggctgggat cattgaagtg gcgcgtctct cctctgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataaacttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt gggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacattccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 ggtgaagtgg tgagccacat tgcatctgat aacgtactgg gcggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgccggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcaccaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgttg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtactggc gatgacagca ttggcgagag cgttcgccag cagctcgcca ctgataatat
     6361 cgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ctctttcccc
     6481 atcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcgttat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaag ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc agccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgtttggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagctctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttactgtt actgtgtacc gaaacgcatc agccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc taccgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggcata
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagatc agcatccata tcgccattgc cggtgattgc aaagagaact
     8341 gagcgatcat tagcccaata atcaccgtga tccccactaa tgtatggcga tagcccagac
     8401 gacgtaagac ttgggtaacc atcgattttg caataatgga acctaacgct gtcggtgcca
     8461 tcatacagcc ggcaataaac gcctgataac caaatcctac ctgtaacatc aatggcataa
     8521 ggaacggtac acagccggtc cccagacggg ttgcaatatt gcctacgata ccgatcgaga
     8581 aagtgcgggt tttaaataaa tctaatgaaa ttaatgggtt tggcgtacgt cgcgcatgga
     8641 gaatatagag aagcagtaac ccgatgctgg tgacaattac cgtcaaggaa atccagctgg
     8701 cgacaatctt ttccccgaat agctctattc cgcttgaaaa gagaacaagg ctgaggccaa
     8761 acagcaaaaa gccagtgata tcgaatctgc gtcgtgcggt ggtgaaattg ggcatatgtt
     8821 tgcgcgcgta aagaaggccc gcaataccta tggggatatt aattaaaaat atccagtgcc
     8881 aggttgccca ggtgaccagc acgccgccaa gaacggggcc taaaattggc cccaccagac
     8941 ccggcatggc gacaaaattc aataccggaa gaagttcatt acgaggataa gcacgcagta
     9001 aggccagccg agcaacaggc atcatcattg cgccgcctat cccttgaata acccggaaga
     9061 caaccagctg tggtagcgaa ttagaaagtg cgcaggccag agaacccaac gtgaacagac
     9121 tcacggcaag ggtaaaaatg cgacgcgtac cgaagcgatc ggctagccat ccgcttaccg
     9181 gaataagcat cgccaccgtc agcgtataac tgatgatggc tgattgcatc gcgagaggag
     9241 aacgattaag gctatgagcg attgcgggta aggcggtatt cagaatagtg gcatcaagtg
     9301 cctgcatgaa gaaggccatc gccgcgatcc acggcaaacc cgacatacta cgcttctttt
     9361 tatcgctcat tcaatgtcct gttatcgggt tatcacttat caggtgagcg tagcagcgcc
     9421 tgacaagctt taaatgccgc gtcgccatcg ctttggataa tcgcatcgac aatcgcctgg
     9481 tgcagatcca gctttatcac tgtgtcgctg gtaattgacg tgaagtaagt gtgataaacc
     9541 gaatggaata gcgaggcgaa tgaggtcaaa aacggattgg cgctcatttc atagatatgc
     9601 tcatgccagg ccatatcgac ttcgatccag cgttcacggc gaaagttctc ttttaatgcc
     9661 gccatttcgg ccattaacgt attgagatgc gccttctgtt ccgcggtgcc aaccgttgct
     9721 gccagtaggc aggcttgcgg ctccagacag atacgcataa ccagaaagtg atcgatgacc
     9781 tgatgaaagt tctcttctgt catccaccag gtaagcaatt cctgatcaag aaaattccag
     9841 ttcgattgtg gcatgacccg agtaccaatt cgcggtcgcg gtaaaaccat cccttttgcc
     9901 attaacgttt tgaccgcttc gcgtaccgct gtacgactca ctccaaattg ctcgcccagc
     9961 tcaatctcac cgggcaaaat ggtgccgggt tcatattcac cttttaagat ccgttgcgcc
    10021 agcttctcag ccagaacata cgaaaggttt ttctgtgcag ctaactgttg tgcgcttaaa
    10081 ggcattactt atcttccttt ttctttttat tcctccttag tatgccacca ggaagtgtga
    10141 ttacggttgc aaaaacggca aattgcttgt tttatggcac attaacgggg cttttgctga
    10201 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    10261 ataatgcgcc accactgaca cggaacaacg gcaaacacgc cgccggggca gcggggttct
    10321 cctgagaatt ccgacagaga aagcaaaaat aaatgcttga ctctgtggcg ggaaagcgta
    10381 ttatgcacac cccgcgccgc tgagaaaaag cgaag
//
LOCUS       DADLSE010000131        10405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLSE010000131 DADLSE010000000
VERSION     DADLSE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.131"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(38..364)
                     /locus_tag="JN692_003240"
                     /pseudo
     CDS             complement(38..364)
                     /locus_tag="JN692_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(374..922)
                     /locus_tag="JN692_003241"
     CDS             complement(374..922)
                     /locus_tag="JN692_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7265473.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(922..1629)
                     /locus_tag="JN692_003242"
     CDS             complement(922..1629)
                     /locus_tag="JN692_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7265474.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAISAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIANGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JN692_003243"
     CDS             complement(1644..2321)
                     /gene="ydjY"
                     /locus_tag="JN692_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7265475.1"
                     /translation="MSQHYSVSWEKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2326..3036)
                     /locus_tag="JN692_003244"
     CDS             complement(2326..3036)
                     /locus_tag="JN692_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7265476.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JN692_003245"
     CDS             complement(3203..4009)
                     /gene="xthA"
                     /locus_tag="JN692_003245"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7265477.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4455..5675
                     /gene="astC"
                     /locus_tag="JN692_003246"
                     /pseudo
     CDS             4455..5675
                     /gene="astC"
                     /locus_tag="JN692_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5672..6706
                     /gene="astA"
                     /locus_tag="JN692_003247"
     CDS             5672..6706
                     /gene="astA"
                     /locus_tag="JN692_003247"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7265478.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            6703..8181
                     /gene="astD"
                     /locus_tag="JN692_003248"
     CDS             6703..8181
                     /gene="astD"
                     /locus_tag="JN692_003248"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HAY7265479.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            8178..9521
                     /gene="astB"
                     /locus_tag="JN692_003249"
     CDS             8178..9521
                     /gene="astB"
                     /locus_tag="JN692_003249"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7265480.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            9514..>10405
                     /gene="astE"
                     /locus_tag="JN692_003250"
     CDS             9514..>10405
                     /gene="astE"
                     /locus_tag="JN692_003250"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7265481.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHD"
BASE COUNT         2392 a         2659 c         2917 g         2437 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattactc ttttaaggtg cggaagtttt
       61 cgccgttcac ccagagcaga tccaccgagc cgcccgtttt acgaccggca gcagcttcgg
      121 tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag
      181 cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc
      241 cgccccaggc gttaaaccag acggtttgcc ctttggcctc atttttaatt tcctgccagt
      301 cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc
      361 gcatcttccc cccttaagaa cgtttttccg gaatacggca cagcccctga gcgggaatgt
      421 ccatggcaga attaaaacga gccgacaggt tttgcaggcc gatcaatgcc gtcagttcgg
      481 tgagcgcctg agcgtcaaaa tgcgcagcca gtcgggtacg cagggcatca tcgactgttg
      541 gcggtgtgac gcttgcggct tcggcgtact ccagcgccag ccgttcttca tcgctaaaga
      601 gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg
      661 cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg
      721 cgctgacaag cgatcgcact accggatcga gcggtgagcg tttgcgctcc agccacccaa
      781 caaacattga caccaaataa aagataaacg ggatccgccc ccaccagcga atcgggctta
      841 gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt
      901 tgctaagcgg cggtaaaccc atcagtcatt cctcttctgg cgctcaagcc atattttctt
      961 cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa
     1021 agtaccgcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc
     1081 aagcccgttg gcgataaaaa acgagcgaaa acgtattgaa gtcagaccgg cagcatagct
     1141 gattggatcg aaagggacaa aaggcaataa ccgacagacc aggatggtgt gtttgccgta
     1201 gcgagtgaaa aagccgtcca tactgtcaag cacggttttg ccggttaatt tttccaccac
     1261 ttcgcggccc atcactctgg cgataaaaaa gcacagcgcc gcgccggcca tcgaactggt
     1321 ccacgacagc aagccgcccc agaacgcgcc aaacagcgac gcattggcaa aggtgatcaa
     1381 aaacgcaggt agcggtgcag aaatggcctg taaaatcatc aacaagaacg agacaactgc
     1441 cgccagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa
     1501 cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc atgcgagcag
     1561 catagcgaac aacaggatga tgagggttat acggtagtac cagattttac gcgattgtat
     1621 cttcatcatc ctttatcctg gctttattcg gtgattttaa aggtaaccgt tgccagcgtg
     1681 ttatccgccg ggagaactga ggcattgcct ttgaatttca cttcgccgcg tttttccacc
     1741 gcaccataag tgtatgttgc attgctgacg atgccgaccg ggcagctatc cagacacacc
     1801 aggcaacccg ttttcttctc ttctgctgcc gttaaattac cgccaaagcg catgtccagt
     1861 ttcttgccat tactgtcaac aatcacttca tcgaaggaat acgcttttgc cgccccttgc
     1921 cagttgaccg aaatatccag tttgctgcct gtgacatgag tcgtttcttt attatccatc
     1981 gtcatgtttt cgcccggcgt accacctgcc tctttcaggg cttcataaaa tgctttcggg
     2041 gtcgcataag ccataaacag cgatttatgt ccgttggagc catctttaaa cacaataccg
     2101 tgacgagtgt cgtcggtgag gaaacgacca ttaatttgca ccagcatggt taccgtatgg
     2161 ttattggcat cgacgcgcag cggttggctg ttcgaaagtc cgtcgtattc cacttttgcc
     2221 gccgcattct ctttttgatc gcagccgcta agcccggcaa cagccagtaa gcagagtgca
     2281 gccagtcctt tttcccatga cactgaataa tgttgcgaca tcgttctatc cttcattttt
     2341 tgggtgagta agtgggctgc ggcgcgaagc agacagatcc acatgtttgt gtcgggcgta
     2401 gagttttgcg agctggatga gaataaacag cgccaggccc gccagacaga gttgtaaaat
     2461 aaagcgcagc gtaatgcctt cattggcgag atcgcttgcc atcacggtat aaataacaat
     2521 accgggcagg gtcgtgagtg ccgaaataag ggtataaggc cagaaggcga tggtggttaa
     2581 tccgtaagcg taattttgaa tattgtaagg aaacaacggg attaagcggg tcagaataag
     2641 aaaatcaata ccgttacgcg caatgccttt ttcaatggcc tggaaggtat ggctatggcc
     2701 cacgtatttc agcagtaaat cacgtcccag ccagcgcgcc agcaggaatg agcacgagga
     2761 ggccagcgtt gcggcaatta atgagagtag tgtccctaag agcgggccaa aaactattcc
     2821 tccagcgatc accagtatgc ttcctggtaa cagaaagagg gtggcattga tgaataacag
     2881 aatatagagg ctataaccga acaatccgct ctggcgaatg agtgtctgta agtggggtaa
     2941 atcggtgagc agatcgaata aaccgaaagc gtggatagcg taaatgacca gcgcaaaaat
     3001 aagacaggca aacagaaagt tacgctcagc gttcatcgtg gagtttccta tatcaaactt
     3061 gataatagag aagtggtcag acaactaaaa acgcagtgac ggtataacaa aggacggcag
     3121 gcaacaaatc attactgaca gtttgttgat ttttctcaag gttaattctc ctgacccagt
     3181 ttgagccagg agagctgcta aattagcggc ggaaggtcgc ccagacgggg gcgtgatcgg
     3241 acggtttttc catgctgcga atttcatagt cgatgccggt ttctacgcaa cattctgcca
     3301 gcggttggct ggcgagcagc aggtcgatgc gcagaccacg gttatcgtca aaaccttttg
     3361 agcggtaatc aaaccatgag aaacgatctg ctgtttgcgg attagcatgg cggaaggtat
     3421 cgaccacccc ccagctcatc agcctgtcca tccattcgcg ctcttccggc aggaaagagc
     3481 atttaccggt acgcagccag cgcttacggt tttcttcgcc aatgccgata tccagatctg
     3541 tagggctgat attcatatcg cccataatca gtaccggatt atcacgtttg agttcggttt
     3601 ccaggtagtt ttgcagattc tgataaaact gcgcttttgc cgggaatttt atcggatggt
     3661 cgcggctttc accctgcggg aagtaaccgt tgatcacggt gacattaccc agcagtgagg
     3721 ggatttccgc cataataatc cgccgctgcg cctcttcgtc gtcaccggga aagccgcgac
     3781 gcacggcaat cggcgtctct ttggtcagca gcgccacgcc ataatggcct ttctgcccgt
     3841 gataaaacac gttgtagccg agcttcgcca cctcttcgag cggaaacata tcgtcatgaa
     3901 cttttgtctc ctgcaggcca atcacatccg gttggtgctt ttcgacgatg gcttcaagct
     3961 ggtgaggtct ggcgcgcagg ccgttgatat taaaagagac aaatttcata gtcgctgcca
     4021 tttattcaga taaagagtgc gtggatggta gcagaatttc gcgttgctta ccgcctgttg
     4081 ttaagtgatt tgatcgatgg ttgttaatga tgtcaacgat ggcgcaaaaa atgcccgctt
     4141 ttggtgcgcg ctgcgtcaga atggcgcagt aatttccagt aaatttcgtc aatgatcaca
     4201 attccagaat tatattttac ttctgcataa cattgcgttt tttattcttt ttattcaccc
     4261 acatcgcaaa cgtattcact ttatatgcac tttaaatgca tatggtttgg ttataactcc
     4321 ttgatttcca gttagcctcc gccgtttatg cacttttatc actggctggc acgaaccctg
     4381 caatctacat ttacagcgca aacattactt attgttaaca tacaaataac gaattattta
     4441 ctgtagaggt cgctatgtct cagccaatta cgcgtgaaaa ctttgatgaa tggatgatac
     4501 ctgtttacgc tccggcaccc tttataccgg tacgtggcga aggttcgcgc ttgtgggatc
     4561 agcaggggaa agagtatatc gacttcgcgg gtggcattgc ggtgaacgcg ctgggccatg
     4621 cgcatccgga actgcgtgaa gcgctgaacg aacaggcgag taagttctgg cataccggca
     4681 acggttacac caacgagccg gtactgcgac tggcgaaaaa attgatcgac gccacgtttg
     4741 ccgatcgcgt cttcttttgt aactccggtg cggaagccaa cgaagcggcg ctaaaactgg
     4801 cgcgtaaatt cgctcacgac cgctacggca gccataagag tggcatcgtg gcgttcaaaa
     4861 atgcgtttca tggtcgcacg ctgtttactg tcagtgcggg tgggcagcca gcctattcac
     4921 aggattttgc gccactgccg gcggatattc gtcatgctgc atataacgat attaactctg
     4981 ccagcgcgct gattgacgac gccacctgtg cggtgattgt cgagcccatc tagggcgaag
     5041 gcggtgtggt gccagccagc aacgcgtttt tacaaggtct gcgtgaattg tgtgaccgcc
     5101 acaatgcctt gctgattttt gatgaggtac aaaccggcgt cgggcgcact ggtgagctgt
     5161 atgcctatat gcactacggc gtgacgcccg atctgttaac caccgccaaa gcgctgggcg
     5221 gcggtttccc ggtcggtgcg ttgttggcaa ccgaagagtg cgcccgcgtg atgaccgttg
     5281 gcactcatgg caccacctat ggcggtaatc cgctggcctc ggcggtggca ggcaaagtgc
     5341 tggagctcat caacacacca gagatgctta atggcgttaa acagcgtcac gactggtttg
     5401 ttgagcgtct taatactatt aatcaccgct atggtttgtt cagtgaagtt cgcggcttag
     5461 gtttgttgat tggctgtgta ctgaatgccg attacgccgg gcaagcgaaa cagatctctc
     5521 tggaagcggc gaaagcaggc gtgatggtac tgattgcggg tggcaacgtg gtgcgttttg
     5581 cgcctgcgct caatgtcagc gaagaagagg tgacgaccgg actggatcgc tttgcagctg
     5641 cttgcgaaca ctttgttagc cgaggttcat catgatggtc atccgtcccg ttgagcgctc
     5701 agatgtctcg gcgctgatgc agcttgccag caaaacgggc ggcggcctga cgtcgcttcc
     5761 cgccaatgaa gccacgcttt cggcgcgtat cgaaagggca atcaaaacct ggcaaggcga
     5821 actgcccaaa agcgagcagg gctatgtgtt cgtgctggaa gatagcgaga caggcaccgt
     5881 ggcggggatt tgcgctattg aggtggcggt tgggctgaac gatccctggt acaactatcg
     5941 cgtcggcacg ttggttcacg cctcaaaaga gctgaatgtc tataacgcat tgccgacgct
     6001 gtttcttagt aacgatcaca ccggcagcag cgagctgtgc acgctgtttc tcgacccgga
     6061 ctggcgtaaa gagggcaacg gctatttgct gtcgaaatcg cgctttatgt ttatggcggc
     6121 ttttcgcgac aagtttaatg acaaagtggt tgctgaaatg cgcggggtga ttgacgaaca
     6181 cggctattca ccgttctggc aaagcctcgg taaacgcttc ttttcgatgg attttagccg
     6241 cgccgatttt ctctgcggca ccgggcaaaa ggcatttatt gcagaactga tgccgaaaca
     6301 tccgatctat acccactttt tatcccagga agcccaggac gtcatcggtc aggtacatcc
     6361 gcaaaccgcg cctgcccgcg cggtgctgga gaaagaaggt tttcgttacc gcaactatat
     6421 cgacatcttc gacggcgggc caacgcttga gtgtgacatc gaccgcgtgc gcgccatccg
     6481 taaaagtcgg ctggtggaag tggcagaagg gcagcctgcg cagggcgatt ttccggcctg
     6541 cctggtcgcc aatgaaaatt atcaccattt ccgcgtggtg ctggcgcgta ccgatccggc
     6601 aaccgagcgt ttgattttaa ccgccgcaca actggatgcc ctcaaatgcc acgcagggga
     6661 tcgcgttcgt ctggtgcgcc tgtgcgcaga ggagaaaaca gcatgacttt atggattaac
     6721 ggtgactgga taacgggcca gggcgcatcg cgtgtgaagc gtaatccggt atcgggcgag
     6781 gtgttatggc aaggcaatga tgccgatgcc gctcaggtcg aacaggcttg tcgggcagcc
     6841 cgtgcggcgt ttccgcgctg ggcgcggctc tcattggctg aacgtcaggt cgttgtcgaa
     6901 cgctttgccg gattgctgga aagcaataaa gccgaattaa ccgcgattat tgccagagaa
     6961 acgggtaagc cgcgctggga agcggcaacc gaagtgacgg cgatgatcaa taaaatcgcg
     7021 atatcaatta aggcgtatca cgttcgtacc ggcgagcagc gtagtgaaat gccggacggt
     7081 gcggcgagcc tgcgacatcg cccgcacggc gtgctggcgg tgtttgggcc gtataatttc
     7141 cctggtcatt tgccgaacgg acatatcgtt ccggcattgc tggcaggtaa caccattatc
     7201 tttaaaccca gcgaactgac accgtggagt ggcgaagcgg taatgcgttt atggcagcag
     7261 gctggcttgc cgccgggcgt gctgaacctg gtgcagggcg ggcgtgaaac gggtcaggcg
     7321 ctgagtgcgc tggaggatct cgacggtttg ctgtttaccg gtagcgccaa taccggctac
     7381 cagctgcatc gccagctctc cggtcagccg gagaaaattc tcgcccttga gatgggcggt
     7441 aataatccgc taattatcga tgaggtggcg gatatcgacg cggctgtcca tctgaccatt
     7501 cagtcggcgt ttgtcacagc cgggcaacgc tgcacctgcg cccgccgttt attgctcaaa
     7561 agcggagcgc agggcgatgc gtttcttgct cgtctggttg ccgtcagcca gcgattaacg
     7621 ccgggcaact gggatgacga accgcagccg tttattggcg ggctgatttc tgaacaggcc
     7681 gcacagcagg tggttactgc ctggcagcaa ctggaagcga tgggcggacg aaccctgctt
     7741 gcgccgcgct tattacaagc agggacatcg ttgctgacac cagggatcat tgaaatgacg
     7801 ggcgttggcg gtgtgccgga tgaagaggtg ttcgggccgc ttttgcgcgt ctggcgttat
     7861 gatacttttg atgaggcgat tcggatggcg aacaacactc gcttcggact cttttgcggt
     7921 ctggtttccc ccgagcggga aaaattcgat caactgttgc tggaggcgcg ggcggggatt
     7981 gttaactgga acaaaccgct tactggtgct gccagtaccg cgccattcgg cggcattggt
     8041 gcttccggta accatcgccc cagcgcctgg tatgccgcag attactgcgc atggcctatg
     8101 gcgagcctgg agtcggactc gttaacattg cccgccacgc ttaaccccgg gctggatttt
     8161 tccgatgagg tggtgcgatg aacgcctggg aagttaattt cgacgggctg gtagggctga
     8221 cgcatcatta cgcgggcctg tcgtttggta atgaagcctc tacccgtcac cgttttcagg
     8281 tgtctaaccc gcgactggcg gcaaaacagg gcttactgaa aatgaaagcc cttgccgatg
     8341 cgggattccc ccaggccgtg atcccgccgc acgagcgtcc gtttattccg gtgctgcgtc
     8401 agttgggatt cagtggtagc gatgagcagg tactggaaaa agttgcacgc caggcaccgc
     8461 actggctttc cagcgtcagt tccgcttcgc caatgtgggt agccaatgcg gcaacgatcg
     8521 cgccatctgc cgatacgctg gatggcaaag tgcatctcac cgttgccaac ctgaacaata
     8581 aatttcaccg ttcgctggaa gcgcccgtca ctgaatcgct gttaaaagcg atttttaagg
     8641 acgaagagaa atttagcgtc cattcggcgt tgccgcaggt agcgttgctc ggtgatgagg
     8701 gggcggcaaa ccacaatcgt ctcggcggtc attacggtga accgggtatg ctactttttg
     8761 tctacgggcg agaagaaggc aatgataccc ggccttcccg ttatccggcg cgacagactc
     8821 gcgaagccag cgaggcggtg gcaaggctta atcaggtgaa tccccaacag gtgattttcg
     8881 cccagcaaaa cccggacgtt atcgaccagg gcgtttttca taatgacgtg attgccgtga
     8941 gtaaccgcca ggtgctgttt tgccaccaac aggcgttcgc tcgccagtca cagttactgg
     9001 caaacctgcg ggcgcgggtg aacggtttta tggcgataga agttccggca actcaggttt
     9061 ccgtgtcgga tgcggtgtct acctatctgt ttaacagcca actgttgagc cgcgatgatg
     9121 gttccatgat gttggtgctg cctcaggagt gtcgggaaca cgccggagta tggggttatc
     9181 tcaatgaact ccttgccgct gacaacccga ttagcgaact aaaagtcttt gacttacgtg
     9241 aaagcatggc gaatggcggc ggcccggcgt gcctgcggtt gcgggtggta ttgacagaag
     9301 aagaacgccg ggcggtgaat ccggcggtga tgatgaacga taccttgttt aatgcgctca
     9361 atgactgggt ggatcgttac taccgcgatc gtcttactgc tgccgatctg gccgacccgc
     9421 aattgctgcg cgaagggcgg gaagcactgg atgtattgag ccaattactg aatctcggtt
     9481 cggtttatcc gttccagcgc gagggagggg gcaatggata attttcttgc tctgacctta
     9541 acgggtaaaa aaccggttat caccgagcga gaaatcaacg gcgttcgctg gcgctggctg
     9601 ggcgatggtg tgctggaact gacgccatta acgccaccgc aaggcgcgct ggtgatttct
     9661 gcggggatcc acggtaatga gacggcacct gtggaaatgc tggacgcgtt gcttggcgcg
     9721 atatctcacg gcgagatccc gttacgttgg cggttgctgg tgatcctcgg gaatcctcct
     9781 gcgctgaagc aagggaaacg ttattgccat agcgatatga accgaatgtt tggcggtcgt
     9841 tggcagctat ttgctgaaag cggagaaacc tgtcgggcgc gcgaactgga acagtgcctg
     9901 gaagattttt atgaccaggg caaagaatct gtgcgttggc accttgatct acataccgca
     9961 attcgtggct ccttgcatcc gcagttcggt gtattaccgc aacgcgacat tccctgggac
    10021 gagaaatttc tgacgtggct gggtgcggcg gggctggagg cgctggtgtt ccatcaggag
    10081 cctggtggta cgtttaccca tttcagcgtc agacattttg gcgcgctggc ctgtacgttg
    10141 gaacttggca aagcgttgcc ctttgggcaa aacgatcttc gccagtttgc agtaactgcc
    10201 agcgcaattg ctgcgctgct atctggtgag agtgtcggta tcgtgagaac accgccgctc
    10261 cgttatcggg tggtttcgca aattactcgc cactcgccgt ccttcgaaat gcatatggca
    10321 agtgacacgc tgaattttat gccgtttgag aaaggaacat tgctggcgca ggacggagag
    10381 gaacgtttta ccgtaaccca tgatg
//
LOCUS       DADLSE010000132        10137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLSE010000132 DADLSE010000000
VERSION     DADLSE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.132"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN692_003251"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN692_003251"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7265482.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN692_003252"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN692_003252"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7265483.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3325)
                     /locus_tag="JN692_003253"
                     /pseudo
     CDS             complement(2698..3325)
                     /locus_tag="JN692_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3455..5215
                     /locus_tag="JN692_003254"
     CDS             3455..5215
                     /locus_tag="JN692_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HAY7265484.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYGKLQRAKAAFWAK"
     gene            5621..6478
                     /gene="focA"
                     /locus_tag="JN692_003255"
     CDS             5621..6478
                     /gene="focA"
                     /locus_tag="JN692_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7265485.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6533..8815
                     /gene="pflB"
                     /locus_tag="JN692_003256"
     CDS             6533..8815
                     /gene="pflB"
                     /locus_tag="JN692_003256"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7265486.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9007..9747
                     /gene="pflA"
                     /locus_tag="JN692_003257"
     CDS             9007..9747
                     /gene="pflA"
                     /locus_tag="JN692_003257"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7265487.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9829..>10137)
                     /locus_tag="JN692_003258"
     CDS             complement(9829..>10137)
                     /locus_tag="JN692_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265488.1"
                     /translation="LLKKRTNGSVRYCQYTSYGDGHKLPFNKVRWVALVGGDKESFVQ
                     MGWEKNISDYLKNMCSYLGIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL
                     "
BASE COUNT         2599 a         2540 c         2404 g         2594 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacagac
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac attaccaggc
     3061 ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc acatcatcat
     3121 aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact tcaaacagcg
     3181 acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc agtacaaagg
     3241 tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta tcaaatgcca
     3301 tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc cgctaaaagc
     3361 atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa aacccgtatt
     3421 attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta tccccggcaa
     3481 agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag acctcggctt
     3541 tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg tacatattcg
     3601 cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga aagcggcgct
     3661 ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt ttgctgattt
     3721 ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg aaaaatggtt
     3781 cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc tgcgcgcatt
     3841 ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat ccggtaacga
     3901 agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga ccgtttatat
     3961 tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg gcaatacccg
     4021 caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga aaaaccgcat
     4081 tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc gttacccagc
     4141 ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg catatgatgg
     4201 ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg caaacggcac
     4261 ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac gtaccgtgac
     4321 cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc caaccttcga
     4381 tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt ccagcggttt
     4441 aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact ggaatttctc
     4501 cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag aagataaaga
     4561 agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta tcgtgcctgg
     4621 catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta tgggcagcca
     4681 tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag attacctgaa
     4741 cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc gtgagctgtt
     4801 gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg aattaaaagc
     4861 catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg aatggacgat
     4921 ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct gcctgcaaac
     4981 gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga atgcgtttgt
     5041 tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg gcgaagcggc
     5101 gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac atcagtcgtt
     5161 gttgaaggcc tacggaaagc tgcagcgcgc caaagcagca ttctgggcaa aataaaatca
     5221 aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg gcgttttttt
     5281 aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa ggttaaatat
     5341 cggtaatttg tatttaataa atacgatcga tattgttact ttattcgcct gatgctccct
     5401 tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt taatttttaa
     5461 aatttatttt tatttggata atcaaatatt tactccgtat ttgcataaag accatgcgag
     5521 ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct ataatgcttt
     5581 gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg acaacccttt
     5641 tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg tctataaagc
     5701 aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt tcatctcaat
     5761 cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct tcggcatggc
     5821 aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg tctgcggagc
     5881 cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc gcatcacctg
     5941 gggtcagttg gctaaaaact ggctaaatgt ctattttggc aacctggtcg gcgcactgct
     6001 gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc aatggggact
     6061 aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg ccgtctgtct
     6121 tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt ctggccgcag
     6181 cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg ccagcggttt
     6241 tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc gcgacttcgc
     6301 atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc acctgaccgt
     6361 gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta tcggcggtgg
     6421 tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc accattaatg
     6481 gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt acatgtccga
     6541 gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact ggcagaatga
     6601 agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg acgagtcctt
     6661 cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg aaggcgttaa
     6721 actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt ccaccatcac
     6781 ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc tgcagactga
     6841 agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg aaggttcctg
     6901 caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg aataccgtaa
     6961 aactcacaac cagggcgtgt tcgacgttta cactccggac atcctgcgtt gccgtaaatc
     7021 cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg gtgactaccg
     7081 tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc agttcacctc
     7141 tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc tgcgcgaaga
     7201 aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga aatacggcta
     7261 cgacatctct ggtccggcta ccaacgctca ggaagctatc cagtggactt acttcggcta
     7321 cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta cctccacctt
     7381 cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac aagaagcgca
     7441 ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc gtactccgga
     7501 atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg gtggtatggg
     7561 cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca ccctgtacac
     7621 tatggggccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac tgccgctgaa
     7681 cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt atgagaacga
     7741 tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct gcgtaagccc
     7801 gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg cgaaaaccat
     7861 gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg gtccgaagtc
     7921 tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca tggatcactt
     7981 catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact acatgcacga
     8041 caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta tccgcaccat
     8101 ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa tcaaatatgc
     8161 gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa tcgaaggcga
     8221 atacccgcag tttggtaaca atgatccgcg tgtagatgac ttggctgttg acctggtaga
     8281 acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc cgactcagtc
     8341 tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca ccccagacgg
     8401 tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc gtgaccagaa
     8461 aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg ctaaagatgg
     8521 tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg aagttcgtaa
     8581 gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca tcgaaggtgg
     8641 tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga tggaaaaccc
     8701 ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt tcaactcgct
     8761 gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta tgtaattaga
     8821 tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag tggggccttt
     8881 tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc caaaacagac
     8941 tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat ctggagaaac
     9001 accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac cgtagacggc
     9061 ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct gtattgtcat
     9121 aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga tttgatgaag
     9181 gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac cgcatccggc
     9241 ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg taaaaaagaa
     9301 ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc ggtgattgat
     9361 gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa cgacgagatc
     9421 caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa atatctggcg
     9481 aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc tgacgatgac
     9541 gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga gaaaatcgaa
     9601 cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga agaatacaaa
     9661 ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg cattcttgag
     9721 cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt attgagttgc
     9781 cgctttttta ttccgcgctt cattctgcta cagcgttttt aacacatctt atagtgcatc
     9841 taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa gttcttcccc
     9901 atcgaatacc actgtattac acaagaaagt gacatcggca tcttcaatac caagataact
     9961 acacatattt tttaaataat cgcttatatt tttttcccag cccatctgga caaatgattc
    10021 tttgtctcct ccaaccagcg ccacccaacg aactttattg aatggtaatt tgtgcccatc
    10081 tccatatgag gtgtactggc aatagcggac actaccattt gttctttttt taagcag
//
LOCUS       DADLSE010000133        10088 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLSE010000133 DADLSE010000000
VERSION     DADLSE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.133"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            61..5301
                     /locus_tag="JN692_003259"
     CDS             61..5301
                     /locus_tag="JN692_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265489.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTSLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDYEARPANGWDLRAEGWLPVWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALKAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNLTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSETEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGKNITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITNTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIHSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPSITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN692_003260"
     CDS             complement(5425..6111)
                     /gene="kptA"
                     /locus_tag="JN692_003260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7265490.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            6258..6852
                     /locus_tag="JN692_003261"
                     /pseudo
     CDS             6258..6852
                     /locus_tag="JN692_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6849..7310
                     /locus_tag="JN692_003262"
     CDS             6849..7310
                     /locus_tag="JN692_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265491.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            7323..8495
                     /gene="iadA"
                     /locus_tag="JN692_003263"
     CDS             7323..8495
                     /gene="iadA"
                     /locus_tag="JN692_003263"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7265492.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            8560..9471
                     /gene="hypT"
                     /locus_tag="JN692_003264"
                     /gene_synonym="qseD"
     CDS             8560..9471
                     /gene="hypT"
                     /locus_tag="JN692_003264"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7265493.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN692_003265"
     CDS             complement(9464..9856)
                     /gene="iraD"
                     /locus_tag="JN692_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7265494.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(9853..9936)
                     /locus_tag="JN692_003266"
     CDS             complement(9853..9936)
                     /locus_tag="JN692_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7265495.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2659 a         2561 c         2486 g         2382 t
ORIGIN      
        1 gcattatatc tccgtgctag aaaaaacaag tttaattctg ttttttcacg gaaggtcact
       61 atggcttcga caaatgcgca tcaaataaaa aataatgatc aaaactcttt atgtggtctg
      121 ggtgacaaaa tacgccgcct gaccgccgga gtttgtttat ttacacaaat ttttttcccc
      181 gtcatggcga cggcacaaaa tgtggtacat gctaaacctc agacgacagt ttcatctgcc
      241 ccccctctac tagaaaataa tacggtgcct tacacgcttg gtgcgctgga atcagcgcaa
      301 agcgttgctg atcgcttcgg tatttcactg gaggagcttc gtcgtcttaa tcagttccgt
      361 acttttgctc gcggctttga taacgtgcgc cagggtgaag aactggatgt tccggcaaca
      421 acctcgcaga aaagccatga gcaacaaaat gccgtaccgc ctgcgaatgg cgaaaacacg
      481 ctggagaatc aaatagccag cacctcgcag cgagttggca ctctgctttc acaagatatg
      541 aatagtgagc aggccagcgg catggcgcgt ggttgggcgt cttcagaagc ctcaggcgcg
      601 atgactgatt ggttaaataa ctttggtact gcgagaatct ctctgggtgt ggatgaagat
      661 tttagcctga aaaattcgca attcgacttc ctgcatccgt ggtatgacac acccgattat
      721 ctgctcttca gccagcatac ccttcaccga acagacgatc gtacccagat caacaccagt
      781 ttgggctggc gtcatttcac ctccagctgg atgtcaggca tcaacctgtt ttttgaccac
      841 gacctgagcc gctatcactc ccgcgcgggg cttggcgcag aatactggcg tgattatctg
      901 aagttgagca gcaacgctta tattggcctg accggctggc gtagcgcacc agaattggat
      961 tatgactacg aagcccgccc ggccaacggc tgggatttac gcgcggaagg ctggttacct
     1021 gtctggccac aactgggggg aaaactggtc tatgaacaat actatggcga tgaagtggcg
     1081 ctgtttgaca agaatgatcg tcaaagtaac ccccatgcta ttaccgcagg cctcaactat
     1141 acccccttcc cgcttctgac tctcagtgcg gaacagcgtc aggggaagca aggtgaaaat
     1201 gacacacgtt ttgccgttga tctgacctgg caacccagca gttcaatgca gaaacagctt
     1261 aatccggacg aagtggccgg acggcgcagt ctggccggta gtcgttatga cctgattgat
     1321 cgcaacaaca acatcgttct ggaataccgc aagaaagagc tgattcgcct gagtctgctg
     1381 gatccagtga aagggaagtc tggagaaata aaaccgctgg tttcctcgct acagaccaaa
     1441 tatgccctga aaggctataa catcgaagcc gctgcgctga aagctgccgg aggtaaagtc
     1501 agcacgtctg gaaaagatat cacggtcacg ctgccaggtt accgcttcac taacacccca
     1561 gaaaccgata atacatggtc gatagacgtt accgccgagg atgtaaaagg taacctgtca
     1621 cggcatgaac aaagcatggt agttattcag gctccgacat taagccagaa agattctctg
     1681 ttatccgtca atccgctaac cgtggctgca gataaaaaat cgacgaccac attgaccgtt
     1741 actgcgcacg attccgacgg aactccggtg ccggggctgg cgctgcaaac ccgcagtgaa
     1801 ggcgttcagg atatcaccct gtctgactgg acagataacg gtgatggtag ttacatacag
     1861 atgctgaccg ccggaacgac atcaggttca gtaacactga cgccgcaaat taacggtgag
     1921 agtgcggtaa aagaatccat cgtcgttaat atcgtccctg ttgtctcatc ccgcgaccat
     1981 tcatcaataa caattgataa cgtatcgtat tatgccggag acgacatcaa ggttagggtg
     2041 gaactgaaag acgacagcaa tcaaccggtt gcatatcaaa aagaggaatt ggtaaaagcc
     2101 gttactgtcg aaaacagcaa acctggcgcc acgattgtct ggcacgaaga gcagccgggg
     2161 gtttatgccg cgaattatcc ggcccataag caagggactg cactaagggc acaacttagc
     2221 cttcacaact ggaatgctcc actgcaatca catatttata acattgaggc aaaccagaat
     2281 aaggctcgcg tcgccacatt atcagcgacg aataatgacg tttacgctga taaaaagaca
     2341 tttaataccc tcactatcaa cgtcactgat gagagtgata atcccctgac aaatcatcag
     2401 gtcaccttta agaatgaaaa aggaagcgcg gaatttgtcg aaccgccgca gcaaaatacg
     2461 gatggatacg gtgttgccac aatcaacatg gtaagccagg ttgcggaaga aaacacgatt
     2521 agcgccacgc tgccaaatgg tttctcacaa cggataattg cgaaattcgt tagcgattcg
     2581 agtacgccaa aattcaaaca actggttgcc gatccagata ccattattgc tggcaacagc
     2641 cagggcagta ctctgaccgc catcgtcaca gactttcata acaacccgtt aaaagatatg
     2701 aaagtgaatt ttgtggcacc tggtggctcg caactggaca acacgaccgc cacaacagac
     2761 cagtccggta ttgtgcgggt gcacctgacc agttcaaaag ctggtagcta ttccgtcgat
     2821 gcctcgcttg aggtggataa aaatattcac cagtcggtca cgatcaccgt ggtcccaaac
     2881 agggaacaat cggtaatgac cttgaatgcc gggtcgggca gtgcgatcgc taacaataca
     2941 aatatcgtta ccctgactgc cagtgtgaaa gatgtttatg gacacccgtt gccggatgag
     3001 gatgtgaaat ttaccttgcc agcctccatg accgggaacc tcacgctaag tagtgaaacc
     3061 gcccgcaccg atgcaaacgg tgatgccgtg gtcacattgc gaggcacaaa agcgggtgag
     3121 tttacagtta cggcgacgct gaccagaaat aataccgttg cttatcagca agtcactttt
     3181 attggggata caaacagtgc gcagctccag ccgctgactg cctcattaaa ttccattgtt
     3241 gcgggtaaca gtacggggag taccctgacg gcaacgatcc tggacgctta ccaaaatccg
     3301 cttaaagacc agttggtcac tttccagagt aacgatgtca ctctaagcga aacagaagtc
     3361 accaccaata cgctgggtca ggcgacggta acaatgacca gcaatattgc cggacaacat
     3421 aacgtcgtgg tgagccggaa agcgcaagct tccgataata aaacgtttag tttatcagtg
     3481 ctgccggatg aaagttcggc gaaggtaata agtataaccg gagccgaaaa aacgataacg
     3541 gtgggcaaaa acatcacgct acggatactc gtccaggacg cgtttaacaa tgtaatcgcg
     3601 ggtcaacgcg tcagattaag tgcgcagcca acaactaaca ttacgatagg cgatacggct
     3661 tacaccgata ataacggtta tgcgtacgtt aaccttctca gcacccaacc tggggtttat
     3721 caggtgacgg caacgctgga caataacagt agtagtaagg ttgacgtgaa tgtggcaaat
     3781 ggcaaactcg agttaacatc atcgaaacca gaaaccacgg tccataatag tgagggtatt
     3841 acgctgaccg caacggcgag aaatgcgcgg ggtgaattga tgccagggca aattatcacc
     3901 tttagcgtaa cgcctgaagg tgcaacgcta agcaatacag gggaagtcct tactgaccag
     3961 tcaggtcagg ccaaagtgac gctgaccagt gacaaagtga atgtctatac cgttacggcc
     4021 ataatgggca aagatgttcc cgttcagagc caggtaacgg ttgcggttaa ggcagatgct
     4081 aaaacggcac atgttgtgag cgtcgtggct tctcctgaca ccatcaccgc cgacggcatc
     4141 gatagcagca ccatcacttc acgagtagaa gatgattacg gattcccggt tgaaggtgtc
     4201 gatgttagtc atggcttaga caccaaaggc agcccggtag ttaatattcc aactacgcgt
     4261 accgatcagt ccgggcaagt cacggcgaca ataaccaata cattggcaga aaccttaaca
     4321 gtcaatgtgc aagttcctgg tacagccaac caatccgcaa ccattacatt ggttgccggc
     4381 acggccgatg aaagtaagtc aattttgaaa tccgatgttg acactctgaa ggctgactac
     4441 cagcagagcg caaaacttac gctaacattg caagacaagt acggtaaccc gatagtgacg
     4501 tctgatcatc tggaatttgt ccagtcaggc cccttcgtga actttctcaa gttgagcgat
     4561 attgattaca gccaaagaaa ttatggcgag tacaccgtga ctgtcactgg cggaaaagag
     4621 ggaacagcga ccctcattcc catgctgaac ggggttcatc aggcaaactt aagcgtatcg
     4681 ctgaatctca tccactcgat aaaagaaatg tccggtcatg tcactgcaaa caaccatacc
     4741 ttctccacgg ctaaattccc gagcgaaggc tttgcagggg cgtattacac actcaacaat
     4801 gataactttg aagcgggtaa aaccgttgat gattatatgt tttcaagttc acagagttgg
     4861 gtgtctgtcg atgcttcagg taaggtttct ttcgcaaata tcggcgatca aacgtcagtc
     4921 acaataagcg ctgttccccg acaaggaggt acaacctacc agaccttaat taagctgaaa
     4981 ggctggtggg tgaataatgg aaatcatacc aatatctggc tagctgccaa tgcgctctgt
     5041 catgctaaaa atgatggata taatcttcct agcatcacac atttgacgtc tggcgaaaac
     5101 aaacgcacgc agggatcgct gtatggtgaa tgggggaacg ttggagcgtt ttccagtaat
     5161 tcgcaattta caccgggagc ttactggaca agtgaatctg atgattacag tcggcactac
     5221 tatgtgcaga tgctaaccgg tatgaccgga agcgacgctg attccagccc ccaactgacc
     5281 gcctgccgta aatcacttta atttcagcag ttgttattaa aggataccct tttacgaggg
     5341 tatcccttca gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta
     5401 tataacccct gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca
     5461 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
     5521 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg
     5581 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc
     5641 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
     5701 gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc
     5761 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
     5821 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
     5881 tatgagctta ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg
     5941 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
     6001 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac
     6061 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
     6121 tttccctatg caaaaaacgc atagccagaa acatacttgc attttcccca tacccacatg
     6181 gctgataagg tttttcagtt ccaggaattt tcctggctca agcaacacac aaacacaaaa
     6241 aaacaatggg gattgttatg acacaacaag gggatgctgt agccggggag ctggcgacag
     6301 aaaaagtggg cattaaaggg tatctggctt tttttctcac cataatcttt ttttccggtg
     6361 ttttttccgg aacagacagc tggtggcgcg ggggctaaag atggctttct ctttgcgctg
     6421 gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga tggtcttggt
     6481 ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt gggcattcca
     6541 gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc cgccggtatg
     6601 accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat tttcgccgca
     6661 tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggagt cgcggtgttt
     6721 gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt taaatttgtc
     6781 ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc aacgcaagga
     6841 gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc gatggcgcgc
     6901 gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg gcgtttgtca
     6961 tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc tgtgaacccg
     7021 taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc gcattaatga
     7081 gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca ttaaccggcc
     7141 acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg caaaatgtcg
     7201 gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc attacggtct
     7261 gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa caaggagtta
     7321 ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat ttgtatgcgc
     7381 cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc gccgttgcca
     7441 gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc gggcagatcc
     7501 tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc gaagcaggtc
     7561 ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc gtcacgtcag
     7621 tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg ctcgccaaga
     7681 cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct tatcatgtcc
     7741 cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat cgtgtgattg
     7801 gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat cacctggcta
     7861 atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc gtcaccgtgt
     7921 tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg gaaaactgcg
     7981 atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg ttgtttgagc
     8041 aggcactgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc attgacgaac
     8101 cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg ctggcacgcg
     8161 tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa gggaatttaa
     8221 cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg ctggtcaaag
     8281 actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta gccggtttcc
     8341 ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta ctggtcatga
     8401 cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc aaagacggca
     8461 aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc cctaatggac
     8521 gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg tggtgcggtt
     8581 ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa atgccgcaat
     8641 ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg ccgcatccgt
     8701 gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc actgcaactc
     8761 tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca gttagaaagc
     8821 aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa gatagccgct
     8881 gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc gccgctcttt
     8941 acctgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg tgaagggcaa
     9001 agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt tgaccacatt
     9061 cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg agaagcactt
     9121 tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc ctacatgggg
     9181 cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac cttttttgtc
     9241 tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat tgcctggctg
     9301 ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct gaatcgggac
     9361 gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat gaatccagtt
     9421 gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg acattctcaa
     9481 gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc ggttcatcgt
     9541 cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct tctttcaaat
     9601 taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc agctgacggc
     9661 aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg ggcatgcctg
     9721 aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg agatcgctgc
     9781 ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa acagcctgaa
     9841 gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg cttgccctga
     9901 acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt gttcattaca
     9961 tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc ttacttgcct
    10021 atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc atcaaactca
    10081 aaaaaaaa
//
LOCUS       DADLSE010000134        10082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLSE010000134 DADLSE010000000
VERSION     DADLSE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.134"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..379
                     /gene="fliR"
                     /locus_tag="JN692_003267"
     CDS             <1..379
                     /gene="fliR"
                     /locus_tag="JN692_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="FliR, with proteins FliP and FliQ, forms the core
                     of the central channel in the flagella export apparatus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY7265496.1"
                     /translation="FLTFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIF
                     LNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAP
                     FCKHLFSEIFNLLADIISELPLI"
     gene            669..1292
                     /gene="rcsA"
                     /locus_tag="JN692_003268"
     CDS             669..1292
                     /gene="rcsA"
                     /locus_tag="JN692_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7265497.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(1336..1578)
                     /gene="dsrB"
                     /locus_tag="JN692_003269"
     CDS             complement(1336..1578)
                     /gene="dsrB"
                     /locus_tag="JN692_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7265498.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            1687..1914
                     /gene="yodD"
                     /locus_tag="JN692_003270"
     CDS             1687..1914
                     /gene="yodD"
                     /locus_tag="JN692_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7265499.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            2212..3027
                     /locus_tag="JN692_003271"
                     /pseudo
     CDS             2212..3027
                     /locus_tag="JN692_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(3024..4718)
                     /gene="yedQ"
                     /locus_tag="JN692_003272"
     CDS             complement(3024..4718)
                     /gene="yedQ"
                     /locus_tag="JN692_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7265500.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGICMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(4889..5071)
                     /locus_tag="JN692_003273"
     CDS             complement(4889..5071)
                     /locus_tag="JN692_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7265501.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(5150..6067)
                     /locus_tag="JN692_003274"
     CDS             complement(5150..6067)
                     /locus_tag="JN692_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7265502.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6240..7160
                     /gene="yedA"
                     /locus_tag="JN692_003275"
     CDS             6240..7160
                     /gene="yedA"
                     /locus_tag="JN692_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7265503.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(7149..7619)
                     /locus_tag="JN692_003276"
     CDS             complement(7149..7619)
                     /locus_tag="JN692_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7265504.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(7600..9018)
                     /locus_tag="JN692_003277"
     CDS             complement(7600..9018)
                     /locus_tag="JN692_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7265505.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(9085..9780)
                     /locus_tag="JN692_003278"
     CDS             complement(9085..9780)
                     /locus_tag="JN692_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAY7265506.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(9820..10077)
                     /locus_tag="JN692_003279"
     CDS             complement(9820..10077)
                     /locus_tag="JN692_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265507.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         2477 a         2653 c         2410 g         2542 t
ORIGIN      
        1 gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac
       61 cctgccgatt ggtagcgaac cgttgaacag caatgcgttt ctggcaccca ccaaagcagg
      121 gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgttgctgac
      181 cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca tttttgttat
      241 tggatttcca ttaactctga ctgtcggcat ctctttaatg gcggcattaa tgccgttaat
      301 tgcgcctttt tgcaaacatt tattcagtga aatttttaat ttgctggctg atattattag
      361 tgaattacca ttaatataat tccgtaacgt ttatcatgct accctaagga ttatccgaaa
      421 aataatatct gcgaacatct tccaggatac tcctgtagcg aaatatttgt tttaagctca
      481 ctcacatatc gcaacattta ctttacttta agacaattcc aggcaaatta tacaacactt
      541 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
      601 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
      661 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
      721 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
      781 accttgccat agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt
      841 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
      901 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
      961 gaaaaaattt attgatcagc tctaaatcga ttaaaccgga atctctcgac gatatccttg
     1021 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
     1081 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
     1141 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
     1201 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
     1261 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttccctccag
     1321 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
     1381 attaaagaac cagatgccga gcgggtagtc ttccagcgaa accaggtaca ttgtgccttc
     1441 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
     1501 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
     1561 atttcgtttt tctggcagcg ccaggcgcgc gcgagtgctg attttctcga cggtctatac
     1621 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
     1681 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
     1741 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
     1801 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
     1861 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
     1921 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
     1981 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
     2041 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
     2101 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
     2161 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
     2221 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
     2281 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
     2341 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
     2401 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
     2461 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
     2521 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
     2581 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
     2641 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
     2701 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
     2761 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
     2821 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
     2881 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
     2941 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
     3001 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
     3061 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
     3121 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
     3181 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
     3241 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
     3301 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
     3361 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
     3421 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
     3481 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
     3541 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggagaggagt
     3601 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
     3661 ccgcgcacgc cttcgcttag cgtatggacg cgcaccagca caccgtcgaa atggtccaga
     3721 cgttcccagc taacatagcg actgtccata caaatgccgc cccgcgtgtc atgttccatt
     3781 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
     3841 ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc atcgaggttt
     3901 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
     3961 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
     4021 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
     4081 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
     4141 cgcgtaaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
     4201 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
     4261 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
     4321 aatgcatccg atacgccatt gactggcaag gtgcgccgtc ggttgagttc gatttgccag
     4381 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
     4441 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
     4501 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
     4561 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
     4621 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
     4681 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
     4741 ttttatgatt ctggcataac ccggcgatag cgtaccgcaa aacagcgata attttataat
     4801 ggtggcatga taagacgcga ctatcattca ttgcaggcat tgggacacca ggccggatgt
     4861 gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga ctcccctccc
     4921 ccggaacaag atcggtttca tgaaaagctt cccgcttgac cccgtaaccg tcataccaac
     4981 gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg ccctctttaa
     5041 ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga gtgaaacttg
     5101 ttcaccttag ttgaagatgg cgaattttgc ataatagcca gtgcgagtat taatgtgcct
     5161 gaccgcgcat tttcgctact gctttcactc ccagcactac gatgccgccg atgataaatc
     5221 caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt tgctgcccgg
     5281 cgatatgttc aatagcgtga tgcagcggcg caataccatg taccacaatc ccgccgccga
     5341 cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccaa ggcgcgataa
     5401 tcaataaccc tttacctaat gcctgcatca gcgcgctgga tttttccgcc agccaatacc
     5461 ccaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg gtcactacca
     5521 gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg cgcctctgcc acaatcccca
     5581 gcgtgatggc gacgatttcc gcagacaaaa taaaatcggt acgaatcgcc cctttaattt
     5641 tgtccttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc tgctggctct
     5701 gcgctggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc tctacccctt
     5761 caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac gggataaacg
     5821 cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat cctttcgcca
     5881 cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt tgcgcattga
     5941 gcgataaatc atcccctaat acaccggcgg ttttcttcgc cgccagtttg cccatcacag
     6001 agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta ctcccggcca
     6061 aaagcattcc ctctcatttt tattgttaaa gggtgtaagt atgaagcaaa aacggcagct
     6121 ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta tatctctcgc
     6181 cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccgccg tgagggatta
     6241 tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat atcatttggg
     6301 gctcaaccta ttttgtcatt cggattggcg tgggaagctg gcctccgtta atgatggcgg
     6361 gcgttcgatt cctggcagcc ggtattttat tgctggcatt tttgctactg cgcggacaca
     6421 aacttccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta ttgctggctg
     6481 tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc atcgccgccg
     6541 tagtgattgc aaccgtgccc ctctttaccc tgtgcttcag tcgcctgttt ggcattaaaa
     6601 cgcgcaaact ggaatgggtg ggtattgcca ttgggcttgc cggaatcatc atgctcaata
     6661 gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc ggctcgatta
     6721 gctgggcgtt tggctcagtt tatggctcgc gtattacctt acctgtaggg atgatggcgg
     6781 gtgcgattga gatgctggcg gcaggcgtgg tgttaatgat cgcatcgatg attgcgggtg
     6841 aaaaactgac ggcgctccct tccctgtcag gcttccttgc ggtcggctat ctggcgctgt
     6901 ttggttcgat tatcgccatt aacgcttata tgtatttaat ccgtaatgtc agtccggctc
     6961 tcgccaccag ctacgcctac gttaacccgg tggtcgcggt cttgctgggt acgggactgg
     7021 gtggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt ttcgcggtgg
     7081 tactggtcac gttgggaaaa tatctcttcc cggccaaacc tgttgtcgcg ccagttattc
     7141 aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct ggcaccttcg
     7201 ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt ctcgcgccta
     7261 cgtaacgcac actcccagac aatcagtacg cgccagccga gctcctgcaa gcgactgata
     7321 tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc agttcgagtc
     7381 gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg ggtaaatatc
     7441 acgcagcgat attcatcaac gacaaaatcc gggcgtccgg gcagactggc gtcctgaacg
     7501 cgaaacgcaa ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat cgccgtatca
     7561 cgcgtggcaa tcgcgcgcat atttttgctg cgggtggcct tatcgtgaac gtcgaccatg
     7621 ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa gcagttttgc
     7681 caccgcggca aagaccggca cgaccaccga gttgccgaac tggcgatagg cctgagtgtc
     7741 cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta agcgcgcgca
     7801 ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt caaagtcttt
     7861 ctcacccgtg gccatatccc aaccgcgatc gattaaaatt tccgcgccat ctttgtaata
     7921 acgagcagac agcgtacggg tgacgctttg cggattgttc ggataaacca ttccataacc
     7981 gaagccgtta ccgcgcgcct gatgtttttt cgcatatcga tagagatact tccacagcac
     8041 cggcgtcagg atatatttcg cctcaaccat cgggtccagc agctgcgcca gcgtcactcg
     8101 ctgtgcaggg aaacattcgc tgatatcacg cagagtaaaa tcggctttca gattcagatc
     8161 gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat gtttaccatc
     8221 gatgattttc ggatcgtccg gcccgttatc ttctgcatcg gcaacgtcat agcccagttc
     8281 gtccagcgtc tgcatgatga tgcggaacgt ttttccctta tcgtgactct tcaggttttt
     8341 gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca ccacatcaaa
     8401 gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg agttcttttt
     8461 cgatacgcca gccagcgaaa atggctgaca agggaaaccg gccagtaaaa catcgtgttc
     8521 aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt cttgatgact
     8581 gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc aataatggtt
     8641 ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc actgtccacc
     8701 aatcgattca aaaccacgac ggatgccgcc aattccggca aatagatcga taaagcgaaa
     8761 cgcataatgc ggatgatgtt ccggtggttt gggtaacaac gtttgcagat gggcaaactc
     8821 tttctcactt aaacggtgcc atgccgagtc attcgccagc gcacgtttta aaattgccgc
     8881 gctccagtga ttctcaccca caccattaag ctgcgccacc agcattttaa catcataaat
     8941 ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg aatcggttac
     9001 tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag gttaacataa
     9061 ttactgccgt ttagggccac gcgctcagcc agcgggtgaa aacgcatcta tcaccttgtg
     9121 atcgatacct tcattctccc ccgccagttc ggcaccgagc ttcgccataa actcgactaa
     9181 aaattgcgcg ttgtgctgtg ccagctgctt gcctcttgcg gtttgcatgg tttgcggtaa
     9241 tttcagtaac tttgtctgga aatgatccag cgcgtagcgt ttatcatcaa gcgggcgatg
     9301 ctgtgcgaac ggatcttcgc catcaaacag cgccaagccc aatgcccctg aaacggcaaa
     9361 tacgcgcgcc aggccaatcg cccccagggc ttccagccga tccgcatcct ggacgatttt
     9421 agcttccgtt gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca
     9481 aacggcctca attttctccg ccggaaattg cacgaactct tcacggagca ggcgacgcgt
     9541 ctcttctgcc gccaggattg aagaacgctg ccgttgcgga tgatttttcg ccaggctcac
     9601 gatatcgtga aaataacagg cggttaaaat caccagcata tcgacatcgt catctgccgc
     9661 cagtttctgt gccgtagccc agacacggcg aaaatgacac aggtcatgcg ccgcgtcctg
     9721 atgttgatga tggtttttca accagttctc gaactgtgcc tgccagtgtt gtaactccat
     9781 acgcgctccg ttgccaaaga gtcgcaacct tagcagtttt tattcatagc gtctgcaacg
     9841 tgcgggacga ggtcgggtgt accatgcaat gccgcctaac aaggcaccaa ccagggccat
     9901 aaacagaaat ccgaccaatg ctcctaacca cttccccgcg gtagatccta aatcactttc
     9961 aaatcccgac acgggggcat cgggtatccg gctcatcacc cagatgtatc gcgccattgc
    10021 ccagacgaaa tagccacacc atgtatagaa agcccacaat gaatcgccac ggataatcta
    10081 ga
//
LOCUS       DADLSE010000135        10015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.135, whole genome shotgun sequence.
ACCESSION   DADLSE010000135 DADLSE010000000
VERSION     DADLSE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.135"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..188)
                     /locus_tag="JN692_003280"
     CDS             complement(<1..188)
                     /locus_tag="JN692_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HAY7265508.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINART"
     gene            complement(193..378)
                     /locus_tag="JN692_003281"
     CDS             complement(193..378)
                     /locus_tag="JN692_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7265509.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(375..3021)
                     /locus_tag="JN692_003282"
                     /pseudo
     CDS             complement(375..3021)
                     /locus_tag="JN692_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
     gene            3229..4218
                     /gene="ldhA"
                     /locus_tag="JN692_003283"
     CDS             3229..4218
                     /gene="ldhA"
                     /locus_tag="JN692_003283"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7265510.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4329..4751
                     /gene="hslJ"
                     /locus_tag="JN692_003284"
     CDS             4329..4751
                     /gene="hslJ"
                     /locus_tag="JN692_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7265511.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4748..5014)
                     /locus_tag="JN692_003285"
     CDS             complement(4748..5014)
                     /locus_tag="JN692_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7265512.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5288..8803
                     /gene="nifJ"
                     /locus_tag="JN692_003286"
                     /pseudo
     CDS             5288..8803
                     /gene="nifJ"
                     /locus_tag="JN692_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            9170..>10015
                     /gene="ompN"
                     /locus_tag="JN692_003287"
     CDS             9170..>10015
                     /gene="ompN"
                     /locus_tag="JN692_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HAY7265513.1"
                     /translation="MKRKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNERDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYVVAN
                     KTQNFE"
BASE COUNT         2547 a         2563 c         2455 g         2450 t
ORIGIN      
        1 tacgttcgcg cattaatttc ggttactaat ttctctgttt cagcatccgt tttcagcgca
       61 accagataac cataaaatgt ttcacctacc cgcccctgag ttctggcttc atccagagtc
      121 aatgccatta cattgctgct taccagccca acaagaaacg cacaaagaag taatgttttc
      181 ttcatcatcg cctcagaaaa gatcgctacg agtttcaagc agctcttcga catctttgtc
      241 tgccttgatg atgatctcat gctcaatttt aacgttcata ttgatagtga tcggttcctt
      301 aggtgcagcg acttcaatgc gaggcgtaca gccaaccagc ataaaagatg acgtcaacgc
      361 agccagtaaa attttcattg tttttcctca cactcttttc cttgcggaca gtcatttccc
      421 ggcagacgtg cgttctgctc caaccatgcc tggagattat cgccaaagcg taaactgcgc
      481 cacagcgtaa aaatattctc ttcatgatgg taattaaggt ttaccgtacc gcttttacca
      541 tcaacgcgac tggtacctgt aatgttggcc tgcatggtta ataaaccgag attatctaag
      601 ttaatttttg tcgatgaccg gctaatttcc atatagcgca accagttaat tgctgaaccc
      661 gcagtcatat tgtctttcac caccgcatct gcggtatctt tatccagccg caaggtcatt
      721 ggcccgctat ttgccagcca accatctttc actatccatt tttcgttgtt tagccataac
      781 ggcaatgcac cactaaatgc cccggacatg gcgaattgtt tcggattgac ggcgctaacc
      841 agttcgctgg atgagagatt attcagccgc aacagcgccg ggtcatgttg cggcatacgt
      901 aattgtttca tcagcacgtt accgcctaac acatcgacgc taacatcagt caacagcaag
      961 ggttcttctt cagtccacgg ataacgccct tgcaaatcag cagtaatatt tttcgctgtc
     1021 accagattaa tcacttcggc aattcgcaac gtaacggggc cgcgagtccc cagatgccag
     1081 gctccatcgg caaaacggaa aggcaggaca aaatcgacac cgttaacctg attatctggc
     1141 atccaggcac tgccgccttt caacacgccg tgccctcccg cgcggaatcc ttgttcaggc
     1201 gcagcagaaa atgcaacctg agcatacagt tcaccatcgc gtaagttcat cttccagtcg
     1261 ggtggcacca gcggctggaa tacagtcagt gattgttttg gccaccaggc gttaccgcgc
     1321 agacgaatac cgtcccagcg accatttacc cgaaccgggc caatctcacc agcatgtaaa
     1381 tcgcctttaa agaggaaata ggtcggatcg cgcccatcga cgctaaattt taaggttgat
     1441 ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg cgtccagtga cagcgcgccg
     1501 ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag aattaatcgc
     1561 ggcttttcta ccgtcatcgt accgtattgt aactggtcga aaccggtgga gagatcggtc
     1621 agcgtaatcg tgctgtcatg ccactcacct tttcctgcga catcccaggt ggcattcatc
     1681 ggtgtaaaac tcccttttcc ccagtagcgc cactgccagc ggccagcgtc cagcgtcagg
     1741 gagaaaatca ttcgccagcc catccatatg cagcacgaaa tcgcccagtt cattttcatg
     1801 cgcctgcaaa atggcctgca aacgtccgtc aacaccacgt tgggtgactt ttacacccgc
     1861 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag
     1921 taacgcgcct ggctcaaagg tcagcgttgg gtcagacaga cttccactta gctgcgcagg
     1981 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
     2041 ttgactgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc
     2101 cgcttgcccc taggtcagca cactcagtcg tccgctgacc agagcgttct caagccctgc
     2161 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacagcca
     2221 gctccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
     2281 caacggatcg ccgttatccc gtgccagcac gatcaattgc ccgctatttt cctgccagtc
     2341 cagctcggca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
     2401 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
     2461 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
     2521 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
     2581 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
     2641 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
     2701 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
     2761 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
     2821 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
     2881 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
     2941 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
     3001 ggctttatat ttacccagca tgggtagtta acatcctgat ttagcgaaaa attaagcatt
     3061 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
     3121 agttgttgat gaatttttca atatcgccat ggctttcaat tatatttgaa attttgtaaa
     3181 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
     3241 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
     3301 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
     3361 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
     3421 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
     3481 gatgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaggcc
     3541 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
     3601 cagcgtaccc gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc
     3661 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
     3721 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc
     3781 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
     3841 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
     3901 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcggcaatt
     3961 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtctatga gaacgaacgc
     4021 gatctattct ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg
     4081 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg
     4141 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc
     4201 ccgaacgaac tggtttaatc ttgccgctcc cctgcaatcc aggggagctg attcagataa
     4261 tccccaatga cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     4321 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     4381 taagtaatga caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg
     4441 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     4501 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     4561 atggtgaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
     4621 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
     4681 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt
     4741 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
     4801 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
     4861 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
     4921 gcgctacgtg cgccgcagtc gcctgctgaa caggctcact actacacgcc gacaacaata
     4981 aagcggcaca ccctacccaa aacgctgctc gcatctcttc ttcctctgat cttcaagcca
     5041 aacgacaccg ccataaataa taggcagcac agagggcggc gtcgagagct gtcctgcgcg
     5101 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
     5161 cattactctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
     5221 tgaaggatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
     5281 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
     5341 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
     5401 ctgggccgga aacggcttga agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
     5461 gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
     5521 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgatgtaca aactggcagg
     5581 cgaactgacg ccatttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactttc
     5641 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
     5701 tgcggctaac gtccaggaag cgcaagactt tgctctcatt tcgcacatcg caacgctgaa
     5761 gagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
     5821 aattgtcccg ctggccgatg acactattct tgatctcatg ccgcaggccg aaattgatgc
     5881 tcatcgcgcc cgggcgctca acccggaaca tccggtgatc cgcggtacgt ccgccaatcc
     5941 tgacacttat ttccagtctc gcgaagccac caacccctgg tacaacgcgg tctatgacca
     6001 tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt cgtcagtatc agccgtttga
     6061 gtattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
     6121 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagttggcg tgctgaaagt
     6181 tcgtctgtac cgtcccttct ccgccaaaca cttgctgcaa gctctgccgg gatccgtacg
     6241 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
     6301 ggatgtgatg accgcactgg cagaagcctt taataatggt gagcgcgaaa ccctgccccg
     6361 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
     6421 ggtatttgcc gagctcaacg cggctaaacc taaagcacgc tttacggttg gtatttacga
     6481 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacact ctgccaaact cggcgaaact
     6541 tgaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcaacca aaaacaatat
     6601 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
     6661 aaaagcgggc ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
     6721 ttatctcatt tcctaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata
     6781 tcagatggct gagcgtttaa aacctggcgg cattttcctg cttaatacgc cgtacagcgc
     6841 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgttaaacc agaaaaaagc
     6901 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
     6961 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
     7021 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggtc aggatctggt
     7081 ggagcgcaac tggcaggctc tggcgctggc gcgtgaatcc gtagaagaag ttccgttgca
     7141 accggtaaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
     7201 cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc
     7261 gctgccgcca gacggcacct ggccgatggg caccactcgc tgggaaaaac gcaatatcgc
     7321 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgccgc
     7381 ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc
     7441 tgccagcctg cattcgctag atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
     7501 gcaggtggca ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
     7561 agaccgtcag aatccagaga ttaaagccat caatatgatg tctcgcctgg aacatgtcga
     7621 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
     7681 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attcaggtgc
     7741 ttgctccggt tgtggcgaga cgccgtatat taaattgctg actcagctct atggtgaccg
     7801 gatgttgatc gctaacgcca ccggctgttc ttcaatttat ggcggtaacc tgccctctac
     7861 accgtatacc accgatgcca acggtcgtgg gccggcatgg gcgaactctc tatttgaaga
     7921 taatgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct
     7981 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgacgg cgttgaaatc
     8041 agacgctacg ccagaggttc gtcgagaaca ggtcgcagct ttacgccagc aactcaacga
     8101 tgttgccgaa gcacatgaac tgctgcgtga cgcagatgca ctggtggaaa aatcaatctg
     8161 gctgattggt ggcgacggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
     8221 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaacaccgg
     8281 tggtcaggcg tcgaaagcga caccgctggg tgcagtaact aagttcggcg agcacggcaa
     8341 acgtaaagat cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc
     8401 tctcggcgcg cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg
     8461 accatcgctg atcattgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag
     8521 ccacgaccag atgcgccaac tcactgctac cggcttctgg ccgctatatc gctttgatcc
     8581 gcgtcgtgcc gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc
     8641 actggaagaa acgttacttc acgagcagcg tttccgtcgt ctgaattcgc agcagccaga
     8701 agtggcagaa cagttatgga aagatgctgc agctgatttg caaaaacgct atgacttcct
     8761 ggcacaaatg gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat
     8821 tatccaattc taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga
     8881 aaatcagtgg caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata
     8941 acccgcgcag aataacgtat agaaaattaa aagtataatt tttattttgt atgtattatc
     9001 ttcgatacat tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact
     9061 taaggcgtaa caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact
     9121 ccccgaaatg gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagaaa
     9181 agtactggca cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt
     9241 ttataataaa gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt
     9301 ttctgataat tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga
     9361 aacccaaatt aacgatcaac ttactggcta cggtcaatgg gaatacaata ttcaggcaaa
     9421 caacactgaa tcttcaaaaa accagtcatg gacccgtctg gcatttgccg gactgaaatt
     9481 tgcagattac ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg
     9541 ctggaccgat atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat
     9601 gactggtcga gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa
     9661 tggtctgaac ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga
     9721 aggcaccaat aacgaacgcg atgttcgtca tgaaaacggt gacggctggg gtctttccac
     9781 aacatatgat ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac
     9841 caatgaccag gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg
     9901 gctaaaatac gatgctaaca atatttacct agcaaccatg tattcagaaa cgcgtaatat
     9961 gaccccgttt ggcgacagcg attatgtagt tgcgaacaaa acccagaact ttgaa
//
LOCUS       DADLSE010000136         9873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLSE010000136 DADLSE010000000
VERSION     DADLSE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.137"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN692_003288"
     CDS             <1..518
                     /locus_tag="JN692_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7265514.1"
                     /translation="SILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKAT
                     GSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSWLVQPVRSEET
                     LHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSA
                     ESVVQCLGRPD"
     gene            634..1194
                     /locus_tag="JN692_003289"
                     /pseudo
     CDS             634..1194
                     /locus_tag="JN692_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN692_003290"
     CDS             complement(1251..2708)
                     /gene="pepD"
                     /locus_tag="JN692_003290"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7265515.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2970..3428
                     /gene="gpt"
                     /locus_tag="JN692_003291"
     CDS             2970..3428
                     /gene="gpt"
                     /locus_tag="JN692_003291"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7265516.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3520..4764
                     /gene="frsA"
                     /locus_tag="JN692_003292"
     CDS             3520..4764
                     /gene="frsA"
                     /locus_tag="JN692_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7265517.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4822..5223
                     /locus_tag="JN692_003293"
     CDS             4822..5223
                     /locus_tag="JN692_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7265518.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN692_003294"
     CDS             complement(5262..6317)
                     /gene="phoE"
                     /locus_tag="JN692_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7265519.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6605..7708
                     /gene="proB"
                     /locus_tag="JN692_003295"
     CDS             6605..7708
                     /gene="proB"
                     /locus_tag="JN692_003295"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7265520.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7720..8973
                     /gene="proA"
                     /locus_tag="JN692_003296"
     CDS             7720..8973
                     /gene="proA"
                     /locus_tag="JN692_003296"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7265521.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIVEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9088..9163
                     /locus_tag="JN692_003297"
     tRNA            9088..9163
                     /locus_tag="JN692_003297"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9121..9123,aa:Thr,seq:cgt)
     gene            complement(9178..>9873)
                     /locus_tag="JN692_003298"
     CDS             complement(9178..>9873)
                     /locus_tag="JN692_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7265522.1"
                     /translation="LRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPE
                     PLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRV
                     LKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDL
                     RHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPN
                     LSQSKNKEGTNDV"
BASE COUNT         2363 a         2457 c         2589 g         2464 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tttcgcatca tggtttgcct gaaggcagtg
       61 acgacgcgct ggcgtttgcg cgttttaatc gccacctgat cgctttgcgc ataatgcaac
      121 aggttaaggc caccggaagt ccggttctgg atgtggcgca taactttgtt agcgcgtgtc
      181 gaatcggtga tcaacaaggg gtcttacatc gtaaaggtgc cacgcctgat gactgtggtc
      241 tggtggttat tcccggttca cgcggtgatt actcctggct ggttcagccc gtcaggagtg
      301 aggaaacatt gcattcgctg gcgcatgggg ctgggcgtaa atgggggcgc accgagtgta
      361 aagggcgtct ggcagcgaaa tacacagcga cgcaactctc gcgtactgaa cttggcagtc
      421 gggtaatttg tcgcgataaa caactcatct tcgaagaagc gccacaagcc tataaatcgg
      481 ctgaaagtgt ggtgcaatgt ctgggcaggc cggattaatt attcctgtcg cgcggctgcg
      541 tccggtgcta acgctcaaaa acagtggagg gaaaaaagga tgatcttgct ataactctcc
      601 tcagcgcagg ggccggaaga atgttgtctc gcagtgaaaa aggcgctgga caggctaatt
      661 aaagaagctg cccgacaaga cgtcgcggta acggtgctgg aaacagaaac aggccgctac
      721 tctgacacgc tgcgttcggc gctgatttct ctggatggcg acaacgcatg ggcgttaagc
      781 gaaagctggt gtggcactat tcagtggatt tgcccgagcc cgtatcgccc acatcacggg
      841 cgtaaaaact ggtttctggg cattgggcgt tttaccgctg atgagcagga gcaatccgat
      901 gcgatccggt atgagacgct gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc
      961 gactcggcgg tacgcgccac gcatttggca tccggtatta gcgtgaaggt tcagtcagag
     1021 cgcagccagc atgctaacaa acggctggcg cgattgctga ttgcctggaa gctggatcag
     1081 cagcaatagg aaaatagcgc ggtgctgaaa tcacagcggc gaatgttcca tcaccagatt
     1141 gaacgtggca acccgcgacg aacgtttaca gggatggctt ttatcgaagg ataatgaagg
     1201 atgaaactgc cggaaggcga ttaaacgcca tccggcagca aatcaaataa ttacttcgcc
     1261 ggaatttctt tcagcagttc agtcagcagt gtccagtaat gacctacgct ttcgatgtga
     1321 acttgctcat ccggagagtg tggaccggtg atagttggcc cgatagaaac catgtccatt
     1381 tccggatacg gttttttgaa cagaccacat tccaggcccg cgtggataat ctggatgttc
     1441 ggcgtcttgt tgaacaggcg ctgataggtt tcacgtacca gatgcatcac cggagaatta
     1501 gcgtccggct gccagccagg atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta
     1561 cccagcgaat ccagcatgct caccacgtag tctttaccgc tgtcgatcag tgaacggatc
     1621 aggcagtgaa tttctacgtt attgtcagtc atggtcacca caccgacgtt cagggaggtt
     1681 tcgaccacac cttttgccac gtcggaattg cggatcacac cgttcggggt ggcgttcagc
     1741 agacgaataa aggtatcgcg agatttcgca atcagggcag ctttatcgtt cgctacagag
     1801 tccagcaaca aggccagatt tttctctttc tctgccagct cgtttttcag gatctcctga
     1861 taggtattca ccagagattt cagtgcgtcg actttatcag ctgcgacagc aatggtcgca
     1921 aaggcttcac gcgggatggc gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg
     1981 tccagttctt ctgcatgacc cgccaggaag cgcaccagca gtttgttggc attacccagg
     2041 ccaacgtgga tttcaccgcc ggagtgaccg cctttcagac cttttaaggt taacttgaag
     2101 gtttcaaaac cagctggaac cgcttcacga tctaaatgca ggttggaggt gaagtcgata
     2161 ccccccgcac aacccatgta gatttcacct tcttcttcgg agtcggtgtt aatcagaata
     2221 tcagcctgca accagttgct ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc
     2281 atggtcagca gcacttccag cgggccgtga accacgtttt cgtcagccag aaccgccagt
     2341 gcagaggcca taccaatgcc gttatccgca cccagcgtgg tgccgcgcgc tttaacccat
     2401 tcgccatcaa tataaggctg gataggatct ttcgtgaagt catgcacggt gtcgttattt
     2461 ttctgcggca ccatatcgag gtgggcctgt aagacgaccg gtttacgatt ttccatacct
     2521 gcggtagcag gtttacgaat caggatatta cctacctgat cgcgttcgac atggaaacct
     2581 ttctcttttg cccaaccaac aatgtattca gcgagttgct cttcatgata ggacgggtga
     2641 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
     2701 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
     2761 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtattgaga ataccacttt
     2821 atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt
     2881 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     2941 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     3001 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     3061 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     3121 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3181 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3241 tggataccgg tggtactgct gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     3301 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     3361 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     3421 gtcgctaatc ttttcaacgc ctggcattgc cgggcgttgt tctttttaac ttcaggcagg
     3481 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     3541 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     3601 aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg
     3661 tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg tgaaatcctc
     3721 gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga tttatacgat
     3781 accgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca ggcgatggtg
     3841 tggcaacaaa aagcctgtac ggaagaagat ccgcaactca gtggtcgaca ctggctgcat
     3901 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     3961 caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct gccgggcacg
     4021 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     4081 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     4141 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact
     4201 attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga ctccagcctg
     4261 ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     4321 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata ccttgaatcg
     4381 ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     4441 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg
     4501 catgatgctt ccgacgaagc gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa
     4561 ggattgctgg ggcgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     4621 ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta
     4681 gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccggc
     4741 tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt
     4801 gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc
     4861 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgcgaaga taatcgattc
     4921 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc
     4981 tggggctggt ggatggagct ggaagcgcag gaatcccgtt ttacctacag ctaccagttt
     5041 ggtctgttcg ataaagcagg cgactggaag agtgttccgg taaaagatac tgaagtggtt
     5101 gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg
     5161 ctgaatctga agctggaacc ggcggatgat tttcgtgatg aaccggtgaa gttaacggcg
     5221 tgagtgaaat gtgccggatg catcacatcc ggcaatattc attaaaactg atacgtcatc
     5281 ccaaccgcga cagtatcatc attattaata ttaagcttat tatcgctatc cagttggttg
     5341 attttataat caacaaacgc tgacatattt ttgttgaaat aatacgtagc accgacgtcg
     5401 atataattga ccagatcttc atcaccgatg ccctcaatat ctttcccttt cgataagaca
     5461 taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc
     5521 tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa
     5581 gttgccagat aaatattatt ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc
     5641 ttgcctgtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc
     5701 ccactaatgg cgaaatcgct gccgccaaag tcatatgtca atgacgtgcc gaagccatcg
     5761 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
     5821 aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
     5881 ttggtcataa agttgtcggt ctgcgccgag gaatcgccac caaattccgg gaacatatcg
     5941 gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa
     6001 tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc
     6061 gctttatttc cggcaaactc cgcttcccac cgaccataac cagtcagttg atcgttaatt
     6121 tgagtttcac ctttaaaacc aaaacggata taactctggt cgccatcttt actggcgtta
     6181 tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt accgtcttta
     6241 ttatatattt ctgcagcctg tacagatgca gatgccacaa tgcccatcac cactaatgcc
     6301 agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat tcagcgggag
     6361 agccccgttg aaaacaggaa agtttttaac ctgagattgt taaagatata ttacagatta
     6421 atgatattct gaaaatgtgg taatttatta aatctgtaat aaaagcgtaa acaactgccg
     6481 ctacgcttgc tgatcccgcg caacaaaacg ccatgctttg ctcgcagatg gttggcaacc
     6541 gacgacagtc ctgctaaaac gttcgtttga tatcattttt cctaaaattg aatggcagag
     6601 aatcatgagt gacagccaga cgctggtggt aaaactcggc accagtgtgc taacaggcgg
     6661 atcgcgccgt ctgaaccgtg cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca
     6721 tgccgccggg catcggattg ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca
     6781 cctgggttac ccggaactgc cagcgactat cgcctcgaaa caactgctgg cggcggtagg
     6841 gcagagtcga ctgattcaac tgtgggaaca gctgttttcg atttatggca ttcacgtcgg
     6901 gcaaatgctg ctgacccgtg ctgatatgga agaccgtgaa cgcttcctga acgcccgcga
     6961 caccctgcgt gcgttgctcg ataacaatat cgttccggta atcaatgaga acgatgctgt
     7021 cgctacggca gagattaagg tcggcgataa cgataacctt tctgcgctgg cggcgattct
     7081 ggcgggtgcc gataaactgt tgctgctgac cgatcaaaaa ggtttgtata ccgctgatcc
     7141 gcgcagcaat ccgcaggcag aactgattaa agatgtttac ggcattgatg acgcactgcg
     7201 cgcgattgcc ggtgacagcg tttcaggcct cggaactggc ggcatgagta ccaaattgca
     7261 ggccgctgac gtggcttgcc gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc
     7321 gggcgttatt ggtgatgtga tggaaggcat ttccgtcggt acgctgttcc atgcccaggc
     7381 gactccgctt gaaaaccgta aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac
     7441 ggtagatgaa ggggcaactg ccgccattct ggaacgcggc agctccctgt tgccgaaagg
     7501 cattaaaagc gtgactggca atttctcgcg tggtgaagtc atccgcattt gcaacctcga
     7561 aggtcgcgat atcgcccacg gtgtcagtcg ttacaacagc gatgcattac gccgtattgc
     7621 cggacaccac tcgcaagaaa ttgatgcaat actgggatat gaatacggcc cggttgccgt
     7681 tcaccgtgat gacatgatta cccgttaagg agctggccga tgctggaaca aatgggcatt
     7741 gccgcgaagc aagcctcgta taaattagcg caactctcca gccgcgaaaa aaatcgcgtg
     7801 ctggaaaaaa tcgccgatga actggaagca caaagcgaaa tcatcctcaa cgctaacgcc
     7861 caggatgttg ctgacgcgcg agccaatggc cttagcgaag cgatgcttga ccgtctggca
     7921 ctgacgcccg cacggctgaa aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc
     7981 gatccggtgg ggcaggtaat cgatggcggc gtactggaca gcggcctgcg tcttgagcgt
     8041 cgtcgcgtac cgctgggggt tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt
     8101 gatgtcgctt cgctgtgcct gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa
     8161 acctgtcgca ctaacgcggc aacggtggcg gtgattcagg acgccctgaa atcctgtggc
     8221 ttaccggcgg gtgccgtgca ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg
     8281 ctgcgtatgg ataaatacat cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa
     8341 ctgtgccgtg aacagtcgac aatcccggtg atcacaggtg gtataggcgt atgccatatt
     8401 tacgttgatg aaagtgcaga gatcgttgaa gcattaaaag tgatcgtcaa tgcgaaaact
     8461 cagcgtccga gcacatgtaa tacggttgaa acgttgctgg tgaataaaaa catcgccgat
     8521 agcttcctgc ccgcattaag caaacaaatg gcggaaagcg gcgtgacatt acacgcagat
     8581 gcagctgcgc tgacacagtt gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa
     8641 gagtatgacg atgagtttct gtcattagat ttgaacgtca aaatcgtcag cgatcttgac
     8701 gatgccatcg cccatattcg tgaacacggc acacaacact ccgatgcgat cctgacccgc
     8761 gatatgcgca acgcccagcg ttttgttaac gaagtggatt cgtccgctgt ttacgttaac
     8821 gcctctacgc gttttaccga cggcggccag tttggtctgg gtgcggaagt ggcggtaagc
     8881 acacaaaaac tccacgcgcg tggcccaatg gggctggaag cactgaccac ttacaagtgg
     8941 atcggcattg gtgattacac cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc
     9001 atttgattca caaggccatt gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat
     9061 tcacgttcgt gaacatgtcc tttcagggcc gatatagctc agttggtaga gcagcgcatt
     9121 cgtaatgcga aggtcgtagg ttcgactcct attatcggca ccattaaaat caataagtta
     9181 cacatcatta gtaccttcct tattttttga ctgggacaaa tttgggaccg atgggttcag
     9241 gatcgagtct atttgccgtg cgtgttcggt aaggtgatta ggtgcaaggt gagcatatcg
     9301 acgaaccatt tcgatagact cccagcctcc catttcctgt aacactgaca acgggactcc
     9361 ggcttgaacc agccaacttg cccaggtgtg tctcaagtcg tgaaatctga aatcatcaat
     9421 accagcccgt ctcagcgccg ctttccaggc tgtgtttgcg tcataccgca tcttccttac
     9481 tgttggcgct ttcgttccgt ctggtttggt acagctttcc ttgtacacaa atacccaacg
     9541 gtgatgattc ccgatttgtt ttttcaaaac gcgacatgca gtatcattca gcgcaacgcc
     9601 gattgcgcgg tttgatttac tctcttccgg gtttatccat gccacccggc gctgcatatc
     9661 tatttgttgc cattcaaggt tgatgatgtt cgagcgtctt aagcctgttg ccagtgcaaa
     9721 ttcaacaaca gactttaatg gctccggaca ttcatcaatc agcctttgtg cttcatgggg
     9781 ctccagccag cggatccgtt tattctttgg ttgaggcact ttaataattg gtgccttatc
     9841 cagcattttc cattcacgct ctgcggctct tag
//
LOCUS       DADLSE010000137         9858 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLSE010000137 DADLSE010000000
VERSION     DADLSE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.138"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            372..1493
                     /gene="ompC"
                     /locus_tag="JN692_003299"
     CDS             372..1493
                     /gene="ompC"
                     /locus_tag="JN692_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7265523.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1605..2660
                     /gene="apbE"
                     /locus_tag="JN692_003300"
     CDS             1605..2660
                     /gene="apbE"
                     /locus_tag="JN692_003300"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7265524.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2734..3798
                     /gene="ada"
                     /locus_tag="JN692_003301"
     CDS             2734..3798
                     /gene="ada"
                     /locus_tag="JN692_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7265525.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3798..4448
                     /gene="alkB"
                     /locus_tag="JN692_003302"
     CDS             3798..4448
                     /gene="alkB"
                     /locus_tag="JN692_003302"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HAY7265526.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     EATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4524..6167
                     /gene="yojI"
                     /locus_tag="JN692_003303"
     CDS             4524..6167
                     /gene="yojI"
                     /locus_tag="JN692_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7265527.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6385..8031
                     /gene="mqo"
                     /locus_tag="JN692_003304"
     CDS             6385..8031
                     /gene="mqo"
                     /locus_tag="JN692_003304"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HAY7265528.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGGAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNRHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGALWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8181..8669)
                     /gene="eco"
                     /locus_tag="JN692_003305"
     CDS             complement(8181..8669)
                     /gene="eco"
                     /locus_tag="JN692_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7265529.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(8754..8969)
                     /locus_tag="JN692_003306"
     CDS             complement(8754..8969)
                     /locus_tag="JN692_003306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265530.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAIIFTRD
                     ISAGLLPHQNLTLKEQVKLSECSLSYS"
     gene            9078..9572
                     /gene="napF"
                     /locus_tag="JN692_003307"
     CDS             9078..9572
                     /gene="napF"
                     /locus_tag="JN692_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7265531.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9562..9825
                     /gene="napD"
                     /locus_tag="JN692_003308"
     CDS             9562..9825
                     /gene="napD"
                     /locus_tag="JN692_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7265532.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQQNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         2395 a         2425 c         2631 g         2407 t
ORIGIN      
        1 atgaatgacg gtaataaata aagttaatga tgatagcggg agttattcta gttgcgagtg
       61 aaggttttgt tttgacattc agtgctgtca aatacttaag aataagttat tgattttaac
      121 cttgaattat tattgcttga tgttaggtgc ttatttcgcc attccgcaat aatcttaaaa
      181 agttcacttg catttacatt ttgaaacatc tatagcgata aatgaaacat cttaaaagtt
      241 ttagtatcat attcgtgttg gattattctg catttttggg gagaatggac ttgccgactg
      301 attaatgagg gttaatcagt atgcagtggc ataaaaaagc aaataaaggc atataacaga
      361 gggttaataa catgaaagtt aaagtactgt ccctcctggt cccagctctg ctggtagcag
      421 gcgcagcaaa cgctgctgaa gtttacaaca aagacggcaa caaattagat ctgtacggta
      481 aagtagacgg cctgcactat ttctctgacg acaagtctgt agatggcgac cagacctaca
      541 tgcgtcttgg cttcaaaggt gaaactcagg ttactgacca gctgaccggt tacggccagt
      601 gggaatatca gatccagggc aacagcgctg aaaacgaaaa caactcctgg acccgtgtgg
      661 cattcgcagg tctgaaattc caggatgtag gttctttcga ctacggtcgt aactacggcg
      721 tagtttacga cgtaacttcc tggaccgacg tactgccaga attcggtggt gacacttacg
      781 gttctgacaa cttcatgcag cagcgtggta acggcttcgc aacctaccgt agcaccgact
      841 tctttggtct ggttgacggt ctgaacttcg ctgttcagta tcagggcaaa aacggtagcc
      901 cggaaggtga aggcatgacc aacaacggtc gtgaagctct gcgtcagaac ggtgacggcg
      961 ttggcggttc tattacttat gattacgaag gcttcggtat cggtgctgca gtttccagct
     1021 ccaaacgtac tgatgaccag aacttcggtc tgaaccgtta tgatgagcgt tacatcggta
     1081 atggcgaccg cgctgaaacc tacaccggtg gtctgaaata cgacgcgaac aacatctacc
     1141 tagctgctca gtacacccag acctacaacg caactcgcgt aggcaacttg ggttgggcga
     1201 acaaagcaca gaacttcgaa gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt
     1261 ccctggctta cctgcagtct aaaggtaaaa acctgggtgt catcaatggt cgtaactacg
     1321 acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc aacaaaaaca
     1381 tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg
     1441 ctggcatcaa cactgataac atcgtagctt tgggtctggt ttaccagttc taatctcgat
     1501 ggatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt
     1561 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
     1621 cccgcgtggc actgctggtt gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac
     1681 ccgccaaaac ccacgctact gaagttactg ttcttgaagg caaaaccatg ggtaccttct
     1741 ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa gaaaagattc
     1801 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
     1861 tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt aagtgaagcg atggccgata
     1921 tcgtcaccac ctcgctgcgc attggggcaa ggaccgatgg tgcgatggat attaccgtcg
     1981 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc
     2041 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacttgacg gtcatcaatc
     2101 agtcgcatca gcaatatctg caaaaagacc tgccggattt atatgtcgat ctctccaccg
     2161 tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc
     2221 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggccagc
     2281 cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg
     2341 atatcaacgg tcatgggatt agcacctctg gcagctaccg taactattac gaactggacg
     2401 gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg
     2461 tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat actggcttga
     2521 tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtttata
     2581 tgatcaccaa agaaggcgat agctttaaaa cctggatgtc accacagttt aaaagcttcc
     2641 ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg tgaccgggca
     2701 gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacatg cttaactgac
     2761 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga attcgttttc
     2821 gccgtgcgca ccacgggtat cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg
     2881 gaaaatgtct ccttctacgc aaatgccagc gaggcactcg ccgccggatt tcgcccctgc
     2941 aaacgttgtc agccagacaa agccaatccc cggcaacatc ggttggataa aatcacccac
     3001 gcgtgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg
     3061 gcgatgagtc catttcatct acatcggttg tttaaagcga ctaccggaat gacgcctaaa
     3121 gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc
     3181 gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta ccgcaaagcc
     3241 gatgaaacgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg
     3301 cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag cgagcggggg
     3361 atttgcgcga tattgctggg cgatgatgac gcgacattaa tcagcgagtt gcagcagatg
     3421 tttcccgctg ccgacaacgc gcctgccgat ctgacgtttc agcaacatgt gcgtgaagtg
     3481 atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggatat tcgcggcact
     3541 gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgtggtga aaccgtcagt
     3601 tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc
     3661 tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gagtggtccg tggtgatggc
     3721 acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct
     3781 gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac
     3841 tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa tgctgcggag caactaatcc
     3901 gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcgct ccaggcggat
     3961 ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgacc catcggcaag
     4021 gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaaa
     4081 gctttcatga tttatgtcaa cgtgaggcta cggcggcggg ctatccagat ttccagccag
     4141 atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat caggataaag
     4201 acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc gcgatttttc
     4261 aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa catggcgatg
     4321 tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg ttgaaagcgg
     4381 ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag gcaggtaaaa
     4441 aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt
     4501 aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct
     4561 ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa
     4621 ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtgttaccgg
     4681 agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa ctggcgctca
     4741 ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag cggattctgg
     4801 atacccacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg ggattaacca
     4861 gcgatgtgcg caatatcacc attgcttttg tgcgtctgcc ggaactgggg caggggatca
     4921 ttctcactat cggttcatcg gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa
     4981 cggctatctg gatggcgatc accatctggg gcggttttgt gctggtggcg cgggtgtaca
     5041 aacatatggc gaccctacgt gaaaccgaag acaagctgta cacggatttt caaactgtac
     5101 ttgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc
     5161 tctacatccc cgatgcgcaa gagtatcgcc atcatattat tcgcgcagac accttccatc
     5221 ttagtgccgt gaactggtca aacatcatga tgctgggcgc tatcggcctg gtgttctgga
     5281 tggcgaacag tctcggttgg gctgatacca acgttgctgc gacctattcg ttgacgctgt
     5341 tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc
     5401 aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagct gagtttccgc
     5461 gcccgcaggc gtttcccaac tggcaaacgc tggagctgcg taacgtgacg tttgcttatc
     5521 aggataacgc gttttccgtt ggtccgatta atctcaccat caaacgtggc gagctgctgt
     5581 ttctgattgg cggcaacggt agcggaaaat cgacgctggc gatgttgttg acgggcttgt
     5641 atcagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagcggc gaacaaccgg
     5701 aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc
     5761 tggggccgga ggggcaaccc gctaacccgc aactggttga gaagtggctg gcgcagctga
     5821 aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag
     5881 ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc
     5941 tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc
     6001 tgccgctgat gcaggagatg ggtaaaacta ttttcgctat cagtcatgat gatcattact
     6061 ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca acttagcgag ctgacgggcg
     6121 aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat tacgacgctg
     6181 acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt ctaataaccc
     6241 tataattaat caacgaaatt ataatgtttc taaaattaga atataattta taaacattat
     6301 ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc
     6361 gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc
     6421 gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact
     6481 gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag
     6541 ctggagcctg aatggtcgat gaccatggtg gagcgcctgg aggggggcgc gcaggagagt
     6601 tcgaacggct ggaacaacgc cggaaccggg cattctgcac tgatggaact gaactacacc
     6661 ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattt
     6721 cagatttccc gccagttctg ggcgcaccag gtcgagcgcg gcgtgctgcg tactccgcgt
     6781 tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgttaatttc
     6841 ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat gcgttactct
     6901 gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccgcaa
     6961 cagaaagtag cggcaacacg cacagaaatt ggcactgatg tgaactatgg tgagatcacc
     7021 cgccagttaa ttgcttcctt acagaagaaa tctaacttct cgctgcaact cagcagcgaa
     7081 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
     7141 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
     7201 ctgaagctgt tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
     7261 ggcggacagt tccttgtttc ggaaaatcca gacgtggtta atcgccatct ggcgaaagtt
     7321 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgtgtt
     7381 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
     7441 aaaaacggtg cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
     7501 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
     7561 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaagcgaa aaaagaggac
     7621 tggcgtttgt ggcaagcggg gcagcgtgtg cagattatca agcgtgatgc cgagaaaggt
     7681 ggcgtactgc gtctgggtac cgaagtggtc agtgaccagc aagggaccat tgcggcgctg
     7741 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttgaatct gctggaaaaa
     7801 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
     7861 tcttatggac gcaagctgaa cggtgatgta gcggcaacag aacgcgagtt gcagtacacc
     7921 agcgaagtgc tggggctgaa atacgacaaa ccgcaagcag cagatagtac gccgaaaccg
     7981 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc
     8041 acatccggca tggtatgccg gatgtggcgt atactgataa gacgcgccag cgtcgcatca
     8101 ggcaaccggc tcgggcgtta gatgttggat gcggcgtaac cgccttatcc aaccaacggg
     8161 gtgccgtact tcacaacagt ttagcgaact tccgcgttgt caattttctc ttccgccttc
     8221 cagacgcggt atttcacatc tacattgtct ggcgtataca ccacgatcgg cagcttgctg
     8281 ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt ctctttcttg
     8341 ccatccgggc aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa gacataataa
     8401 tcatagcccc agccttccag cgttttgctt tccagcttcc cgccgagacg atgcaaattg
     8461 caatcgactt ccagcgtctg accgattaac agttctactt tcagggtaga ttcatcttct
     8521 tgcggggtta actgaatcac ctgacgcttc atgcctgttt cagcttgtgg ataaggcgcg
     8581 attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtagc gaaagcggca
     8641 aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat ttataggttt
     8701 tcaccgatga tgtcgctgaa ggttaacata ttcatcagat ttattaattt gctttatgaa
     8761 taacttaaag aacattcaga caattttacc tgctctttta gtgttaaatt ctgatgaggg
     8821 agcaaaccag cagaaatatc tctggtgaat ataatcgcta tataaatatg tttataacca
     8881 tttgaaatgt gagcaaaagc ccgtttttgc cacgcttccc gccactcttt tgatcctgct
     8941 acaggtttta ccccgatcgg ggtatgcatc tttgacacat cctttaatat cttagcggct
     9001 ataaaaatgg cttattaatt atgcggtcta tttggtcgct ctcaattttc agagcgcgtt
     9061 aatgatggaa ggtcaatgtg aagattgatg catcccgtcg gggcatactc actggtcgct
     9121 ggcgcaaagc cagtaacggt atccgtccgc cctggtccgg tgatgaatct cattttctga
     9181 cccattgcac ccgctgcgac gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg
     9241 gcacgggtgg ctatccgagc gttaatttca aaaataacga gtgcagcttc tgttacgcct
     9301 gcgcgcaggc ttgccccgaa tcgctatttt ctccgcgcca caccagggcc tgggatttgc
     9361 agttcaccat cggggacgcg tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg
     9421 atagctgtga gccaatggca attatctttc gcccgacgct ctccgggatc taccagccgc
     9481 aacttaatag ccaactttgc aacggatgtg gagcatgtgc agccagctgt ccggtatcag
     9541 ccataactgc ggagtatctc catgcacact aactggcaag tttgcagcct ggtcgtgcag
     9601 gccaaaagcg aacgaatttc agacatcagc acccaacaga acgcctttcc cggctgtgaa
     9661 gttgctgtca gcgacgcgcc gagcggtcag ttgattgtgg tggtggaagc agaagacagc
     9721 gaaacgctga tccaaaccat tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg
     9781 ctggtttatc accagcagga agagcaaggt gaggaaacac catgaaactc agtcgtcgta
     9841 gctttatgaa agctaacg
//
LOCUS       DADLSE010000138         9798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLSE010000138 DADLSE010000000
VERSION     DADLSE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.139"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..337
                     /gene="tqsA"
                     /locus_tag="JN692_003309"
     CDS             <1..337
                     /gene="tqsA"
                     /locus_tag="JN692_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7265533.1"
                     /translation="NYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGN
                     ILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIA
                     VLLSDLNKE"
     gene            complement(362..1750)
                     /gene="pntB"
                     /locus_tag="JN692_003310"
     CDS             complement(362..1750)
                     /gene="pntB"
                     /locus_tag="JN692_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7265534.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(1761..3293)
                     /gene="pntA"
                     /locus_tag="JN692_003311"
     CDS             complement(1761..3293)
                     /gene="pntA"
                     /locus_tag="JN692_003311"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7265535.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQSQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            3817..4761
                     /locus_tag="JN692_003312"
     CDS             3817..4761
                     /locus_tag="JN692_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7265536.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4947..6329
                     /locus_tag="JN692_003313"
     CDS             4947..6329
                     /locus_tag="JN692_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7265537.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVSVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            6366..7088
                     /gene="folM"
                     /locus_tag="JN692_003314"
     CDS             6366..7088
                     /gene="folM"
                     /locus_tag="JN692_003314"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7265538.1"
                     /translation="MDKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTVPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(7085..7420)
                     /locus_tag="JN692_003315"
     CDS             complement(7085..7420)
                     /locus_tag="JN692_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7265539.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            7549..8292
                     /gene="rstA"
                     /locus_tag="JN692_003316"
     CDS             7549..8292
                     /gene="rstA"
                     /locus_tag="JN692_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7265540.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            8517..>9798
                     /locus_tag="JN692_003317"
     CDS             8517..>9798
                     /locus_tag="JN692_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HAY7265541.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGGGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSMMSGVISARLLKD"
BASE COUNT         2427 a         2476 c         2343 g         2552 t
ORIGIN      
        1 taattacatc cctaatattg gttcagttct cgcggcaatc ccccctatcg ctcaggtact
       61 ggtgtttaat ggcttctacg aagcgttgct ggtgctggcg ggatatctgc tgattaatct
      121 ggtcttcggc aatattctgg agccgcgcat catgggacgt gggctggggc tttcgacatt
      181 ggtggtattt ttgtcgttga ttttttgggg atggttgtta ggaccggtgg gtatgctgct
      241 ttccgtgccg ttgacaatta ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag
      301 catcgccgtt ctgttaagcg atctcaataa agagtgacgg cctccgcaga ggccgtcagg
      361 gttacagagc tttcaggatt gcatccacgc tggctttggc gtcaccaaac agcatgtggg
      421 tgttttcctt gaagaacagc gggttttgca caccagcata gccagtgttc atcgaacgtt
      481 taaagacaat cacgttctgc gctttccaca cttccagcac aggcatacca gcaatcggac
      541 tcttcggatc atcctgcgcc gccgggttaa ccgtatcgtt agcaccaatc accagtacgg
      601 tatcggtatc agcaaagtca tcgttgatct cgtccatttc cagcacgatg tcatacggta
      661 cttttgcttc agccagcaat acgttcatat gtccaggcaa acgccccgca accgggtgga
      721 taccgaaacg cacattaata ccacgagcgc ggagtttctc ggtaatttca gcgacaggat
      781 attgcgcctg cgcgactgcc atgccgtacc ccggagtaat gatcactgaa tgggagtttt
      841 tcagcagttc cgctgtctct tctgcggtga tttcgcggtg ctcacccact tcctgatcat
      901 cgccagtaga agagccgtcg gtgccgaaac cacccgcaat aacgctgata aaggaacggt
      961 tcatcgcctt acacataatg taagaaagga tagcccccga agaaccgacc agcgcaccgg
     1021 tcacaatcag caggtcgttg ctgagcataa agcccgcagc cgcagccgcc cagccggagt
     1081 acgagttcag catcgacacc accaccggca tatctgcacc accgatggag gcgactaaat
     1141 gccagccgaa taccagcgca atggcggtca ttatcagcaa tgccagcact tgcaggccga
     1201 cgctgtccgt gcgaacaaat acaatcagca gcaagaagga aacgaccaga gccgccaggt
     1261 tcattttgtg acggtttggc agcatcaacg gtttagacga aatcttgcca cacagtttgc
     1321 cgaacgccac caccgaaccc gtgaacgtta ccgccccgat gaagataccg aggaacactt
     1381 ccgtcaggtg aatattgacc agaatcggtg ccattcccgc gtcatgatgc agataactgt
     1441 taaagccaac cagcactgcc gccagaccca cgaagctgtg caggatcgcc accagttctg
     1501 gcatttcggt catttcaact ttcttcgcca gccggatacc aattgcccca ccaatgacca
     1561 tcgccagcaa gatccagcca acattacccg tatccggtcc aaaaatggtt gcgattaacg
     1621 caatcgccat cccggcgata ccgaagttgt taccctggcg agacgtttca tgtttcgaaa
     1681 gaccggccag actgaagata aacaggatcg cggcaacaat gtatgcagct gtaactaatc
     1741 ctccagacat atgttacccc ttaatttttg cggaacattt tcagcatgcg ctgagtcacg
     1801 gtgaagccac cgaaaatatt aatgctggct ataagcaccg cgataaaact aaggaagcta
     1861 acccagccgc cctggccaat ctgcaacagt gctccgacaa caataatccc tgaaatcgcg
     1921 ttggtaaccg acatcaacgg tgtatgcagc gcgtgcgata cattccacac cacgtaataa
     1981 ccgacaacgc aggccagcgc gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg
     2041 cttgacagcc aaccgaacag gatgattgcc agcgccatca acgcgtattt acgccacggt
     2101 gagcaggcac atttttcctc agttttcact tccggtgccg ctttttgtgc cgcctgcgac
     2161 tgagctgata cctgaatcgg cggtgccggc caggtaattt cgcccgcacg gatcacggtc
     2221 acgccgcgaa tcaccacatc atcaaaatca acagtgatat tgccgtcttt ctctttgcac
     2281 aacagtttca gcagattaac gaggttagta ccgtaaagct gtgaggattg cgtcggcaga
     2341 cggcccggaa gatcggtata accaatcact ttgacaccat tttccgtact gaagatttca
     2401 cccggcacgg tgtattcaca gttgccaccg ttttgggctg ccaggtcgac aatcacactg
     2461 cccgccttca tggagtcaac catttcacgg gtaattagct tcggcgctgg tttgcctgga
     2521 ataagcgcgg tggtgacaat gatatcgacc tcttttgcct gggcggcaaa gagttccatt
     2581 tccgctttga tgaacgcgtc cgacatcact ttggcatagc catcgccgct gcccgcttcc
     2641 tctttaaaat ccagctcgag gaattccgcg cccatacttt gaacttgttc tttcacttcc
     2701 gggcgggtgt cgaatgcacg cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc
     2761 agacctgcaa caccggcacc aatcaccatc acttttgccg gtggcacttt cccggccgca
     2821 gtgatttgcc cggtaaagaa gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa
     2881 ccggcgatat tcgccatcga gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc
     2941 acagagtcca tcgccatcac ggtcacgtta cgttccgcaa gtttttgcat taattccgga
     3001 ttctgcgcag gccagataaa actcaccagc gttgtcccgg gattcagtaa cgcaatttca
     3061 tcatctaacg gcgcattgac cttcagaatg atctctgact gccagacgct attcccttct
     3121 acaatttcag cgcccgcttg cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg
     3181 ccgctctcta ccgcgacggt aaaacccagt ttcagcagct gttccactgt ttttggcgtt
     3241 gctgcaacac gggtttcatt ggttaaccgt tctcttggta tgccaattcg catgatattc
     3301 ccttccatcg gttttattga tgatggtttg cctgtgtcag gagccacaca agccgctcat
     3361 gtacgagcta aatgttactc cgttaaaata aattagtaac aaacgcccta taacgtactg
     3421 aaaattatgc ctgtgatcta gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat
     3481 taacgattca gccgtgatag tgggataaac accttagaac gccggataaa gactgataat
     3541 tgtcttcgac ggtcgggtaa aacgagacat cgccccggca cgaatcacta cttaacatta
     3601 aattaactta tacaattcag ttgcttcagt agtaatgatg ctgatacggc tgttttttaa
     3661 gcatagacgg tcatttgagc aggattaaaa ttggtttaag gaatgtgata tgaaaaatga
     3721 cgcagacagt tacaccgttt aaatgcaata atcagccacg tttctcgtta ataacaatac
     3781 cagtacctgg tttgcgcaag gcgaaggatt atttttatga agcttaagaa caccctcctg
     3841 gcgtcggcac tgctttctgc tacggcattc tccgttaacg cagcaacaga actgacaccg
     3901 gagcaagcgg cagcggttaa accttttgac cgtgtagtgg ttaccggtcg ttttaatgct
     3961 attggcgaag cggtgaaagc cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt
     4021 tatgttgtcg acacttctga ttttggtaac agtggtaact ggcgtgtggt cgctgacctc
     4081 tataaagccg atgctgaaaa agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc
     4141 gaactgccga aagatcaggc tgttctgatt gaaccgtttg acacggtcac cgtccagggc
     4201 ttctatcgta gccagccaga agtcaatgat gccatcacca aagcggcaaa agcgaaaggt
     4261 gcctactctt tctacatcgt tcgtcaaatc gatgccaacc agggcggcaa ccagcgtatt
     4321 actgcattca tctataaaaa agatgctaag aaacgtatcg tccagagccc ggatgtgatc
     4381 ccggcagatt ccgaagcagg acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa
     4441 gttgagatcc cgggtgttgc gactaccgca tcaccaagtt ctgaagtcgg tcgcttcttt
     4501 gaaacccagt catcaaaagg cgggcgttac accgtcacgc tcccggatgg cactaaagtc
     4561 gaagaactga acaaagcgac agcagcgatg atggtcccgt tcgacagcat caaattctct
     4621 ggcaactacg gcaacatgac cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa
     4681 ggtgctaagt attaccacat cacccgccag tggcagaaac gtggtaataa cctgaccgtc
     4741 agcgcagatc tgtataaata actattcagt tactcctata aggcggcttg atgccgcctt
     4801 tcgcattttt agtcacaaat ttccccgcga ccattgcatg cagacacatc tcttcgtaaa
     4861 atcccgcgct tcagtggcaa actccatttt tatgcgtttt gccctaatat ttattcttct
     4921 tatcacgttt taatcactgg atatcgatgg aaaagaaact gggactgagc gcactcaccg
     4981 cgctggtatt aagctcaatg ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg
     5041 cagttgccag cccggcagca ctgctcatcg gctggggtat tactggcgct ggcattttat
     5101 tgctggcctt tgccatgctg atcctcacgc gcattcgtcc cgaacttgat ggcggtatct
     5161 ttacctatgc ccgcgaaggg tttggcgagc taatcggctt ttgttccgca tggggatact
     5221 ggctgtgcgc ggtcatcgcc aacgtctcct atctggtgat cgttttttcc gcgttaagct
     5281 tttttacgga cacgccggaa ttgcgcctgt ttggcgatgg taatacctgg caatcgatag
     5341 tcggtgcatc ggctttattg tggattgttc actttttgat cctgcgcggg gtgcaaaccg
     5401 ctgccagcat taacctggtg gcgacactgg caaaattgtt gccgctgggt ctgtttgttg
     5461 tgctggcgat gatgatgttc aaactggata ccttcaagct cgacttcacc ggacttgcac
     5521 ttggcgtatc tgtttgggaa caggtgaaaa acaccatgct gatcaccctg tgggttttca
     5581 ttggtgtgga aggtgccgtg gttgtttctg cgcgtgcgcg taataaacgt gatgttggca
     5641 aagcgacact gctggcggtt ctctccgctc tgggcgttta cttgctggta acgctgcttt
     5701 cgctgggtgt ggtggcacgt cctgaactgg ctgaaattcg taacccgtca atggccggtc
     5761 tgatggtgga aatgatggga ccatggggcg aaatcatcat cgctgccggt ttgatcgttt
     5821 ccgtttgcgg cgcgtactta agctggacca tcatggcggc agaagttccg ttcctggctg
     5881 ccactcataa agcattccca cgcatttttg cgcgccagaa cgcacaagct gcgccatccg
     5941 cctcactgtg gttgaccaac atctgcgtgc aaatttgtct ggtactcatc tggctgaccg
     6001 gttccgatta caacacgttg ctgaccatcg cctcagaaat gattctggtg ccctatttcc
     6061 tggtcggcgc gttcttgctg aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg
     6121 gtgcctgcat ttatggctta tggttattgt acgcctctgg tccaatgcac ttgctgttat
     6181 ccgttgttct gtatgcaccg gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac
     6241 atgataatgt actgaaccgt caggaaatgg tgctgattgg catgctgtta attgcctcag
     6301 ttccggcgac ctggatgctg gtgggataac gttcatccca tcgttggata gcaaaggaga
     6361 taacgatgga taaaacccag cccttgccaa tattaattac tggcggaggt cgtcgcatcg
     6421 gcctcgccct cgcatggcat ttcattaatc aaaagcaacc ggtgattgtc agctatcgga
     6481 cacactatcc agccattgat ggactgatta atgcaggtgc gcagtgtatt caggctgatt
     6541 tttcgaccaa cgacggtgtg atggcgtttg ccgatgaagt actaaaaacc acccatggtc
     6601 tgcgtgctat tttgcataac gccagtgcgt ggattgcgga aaaaccgggt gcgccactgg
     6661 ccgacgtact ggcttgcatg atgcagatcc acgttaatac cccatacctg ctcaaccatg
     6721 cgctggaaag attactgcgt gggcacggac acgccgccag cgatatcatt cactttaccg
     6781 attatgtggt ggagcgcggt agcgacaaac atgttgcgta tgctgcaagc aaagcggcac
     6841 tggataatat gacccgctcg tttgcccgca agctggcacc ggaagtgaaa gtgaattcta
     6901 ttgcgccatc gctgatcctg tttaatgaac atgatgatgc cgaatatcga caacaggcgc
     6961 tgaataaatc actgatgaaa accgtgcctg gcgagaaaga agtgatcgac ctggtcgatt
     7021 acttacttac cagttgcttt gtcaccggac gcagtttccc acttgatggc ggtcgtcatc
     7081 tgcgttaatg cagctttatc cagcatataa tcaacaccca tgcgcttatc ccccaacacg
     7141 caacagaacc aacaaaagcg gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca
     7201 ccaggtaaac aaaataggga ataatcgacc acatactaaa aatgatggtt gcgcgtaagg
     7261 cttcaatgcc gcgttcgctg gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa
     7321 gtggaatcag cccggcgata taataatttt tcgtttttgc taaaacacca atcaacagca
     7381 ctaccagcgc accgagcgcg gctttgatta ccagccccat ctttttacct taacacttcc
     7441 ataacaagtc atcagtagaa tacctgatga aaacttgttt agaaacgatt gatagtaagt
     7501 aaaaacagcg cggtgtattg tgacgttttt atatctaccg tgaatgttat gaacactatc
     7561 gtatttgtgg aagatgatgc ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat
     7621 gatatgcagg ttaccgtaga accgcgcggc gaccaggccg aagaaaccat tttgcgagaa
     7681 aatccggatt tggtgttact cgacatcatg ctaccaggca aggacggcat gaccatttgt
     7741 cgtgatttac gcgcaaagtg gtctggaccg attgttcttc taacctctct cgatagcgat
     7801 atgaaccaca tcctggcact ggaaatgggt gcctgcgact atattctcaa aacgacgccc
     7861 cctgctgttt tgctagcgcg tttacgtttg catttgcgtc agaatgagca agccacactg
     7921 accaaaggtc ttcaggaaac gtctctgact ccctacaaag ccctgcattt cggcacgtta
     7981 actatcgacc ccatcaaccg cgtagtcacc ttggctaaca ctgaaatctc gctctcgaca
     8041 gctgatttcg aattattgtg ggaactagct acgcatgccg ggcaaatcat ggaccgcgat
     8101 gcattgctga aaaatttacg cggcgtcagt tatgacggac tggatcgtag cgtggacgtg
     8161 gctatttcgc ggttaagaaa aaaactgctc gataacgccg cagaacctta tcgcattaaa
     8221 actgtgcgta acaaaggcta tctttttgcg cctcatgcat gggatagtga caataaacaa
     8281 tcagatgact aatatttgtt catctgaaaa agagaacaat taacgaattc aattaataag
     8341 agcttattca aattccaaac cccctacatt tgatatgaat atgagaaaaa atttctctat
     8401 aatcgcctcg tccctgcgtc ttatggcgca gattttttac aacaaactct ttccggcatt
     8461 tcaggtgctg aatgaggcaa gaggcttaaa aaacggttac aactttcaca tatattgtgc
     8521 tttttttgat ctgctcatgg gggattttca aaacggatca aaagagcgaa tccctttcca
     8581 aaagtgtttt tatcgcgatt ttcctggggg ctatcattgg tatctccctg catttcattc
     8641 ctgagacaag cagtgaaacc gttatcgaat ggtataatat tgctggtaac ggttatgtta
     8701 atttattaaa acttgttgcc gttccactca tattcatttc gattctttca gcgattaata
     8761 agctcgaaaa tagcgctggt attggcaaga tgtctttaac tattgttgcc tgtatgttgt
     8821 gtttagtcat gattgcaggt ttagttggtc tggctattgc cagtgcaatt ggccttgatg
     8881 ccagcgcctt tggtcatatg caatcctctt taaccagcga tgacgtctcg aaaacagctg
     8941 cggtatcttt accgcgactc attacgtcat tgatcccaac caacctcttc cttgatctga
     9001 ccggggcaag aagtgtttcg gttattggca tcgtcatctt tacaattctt gctggtattg
     9061 cgctgttaaa agtaaagaaa gatgcaccag cagagggcga aaaattgagc gcggggatca
     9121 acgcgattca aatatgggta atgaaaatgg tccgtattgt cattgcgctg acgccttatg
     9181 gggtaatggc actaatggcg aaagtcttct caacgtatca gctttcacag tttaccaatt
     9241 tgctcgggtt catcgtcgcc tgttatctgg ccgtttttgt gatgtttatt gttcactcgg
     9301 gcattttgct gttaataggt aaaaaccccg ttaactatta caagaccgtc tggcaagtac
     9361 ttacctttgc gtttgtttct cgcagtagtg cggcttcaat tcctctggct atttctgcat
     9421 tggaaaaatt gggggttcca aatacgattg ctaatattgc agcgtctttt ggttccagca
     9481 tgggacaaaa tggctgcgct gggatttatc ctgccgtgat ggtggcgatg attgctccaa
     9541 caatgggtat cgatcccttc tcattgcaat tcctcgcaac tcttttacct gccatcgcgc
     9601 ttggctcaat aggggtagca ggcgtaggtg gtggtgggac attcgcggcg ctgattgtcc
     9661 tttcaacact taatttccct gtcgcattag tagttatttt tattgccata gagccgatcg
     9721 ttgatatggc aagaacagcg cttaatgtga atggttcaat gatgtccggt gtaatatcag
     9781 cccgtcttct taaggata
//
LOCUS       DADLSE010000139         9734 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLSE010000139 DADLSE010000000
VERSION     DADLSE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.140"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN692_003318"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN692_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7265542.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN692_003319"
     CDS             1828..3147
                     /locus_tag="JN692_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7265543.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3524..4669
                     /locus_tag="JN692_003320"
     CDS             3524..4669
                     /locus_tag="JN692_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7265544.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFYSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDCDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKGSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="JN692_003321"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="JN692_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5873
                     /gene="ddpX"
                     /locus_tag="JN692_003322"
     CDS             5469..5873
                     /gene="ddpX"
                     /locus_tag="JN692_003322"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HAY7265545.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACVDNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRRPQQAQAMLWQACPDPQYVVDVTVGSNHSRG
                     TALDLTLRDDVEVVNDAKRGNVRRTPTKTNAI"
     gene            5818..6714
                     /locus_tag="JN692_003323"
     CDS             5818..6714
                     /locus_tag="JN692_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7265546.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6711..7697
                     /locus_tag="JN692_003324"
     CDS             6711..7697
                     /locus_tag="JN692_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7265547.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7690..8616
                     /locus_tag="JN692_003325"
     CDS             7690..8616
                     /locus_tag="JN692_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7265548.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKSTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTASAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHECEVRQSLAIREDGRELRCWRAL"
     gene            complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN692_003326"
     CDS             complement(8672..9103)
                     /gene="osmC"
                     /locus_tag="JN692_003326"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7265549.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            9449..9664
                     /gene="bdm"
                     /locus_tag="JN692_003327"
     CDS             9449..9664
                     /gene="bdm"
                     /locus_tag="JN692_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HAY7265550.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
BASE COUNT         2414 a         2364 c         2419 g         2537 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cttggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccacca
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctattc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggattgcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagg ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg ccgcccacaa caagcacagg
     5701 cgatgttgtg gcaagcctgc ccagacccgc aatatgttgt tgatgtgacg gtcggttcta
     5761 atcacagccg tggcacggcg ctcgacctga cgcttcgtga tgacgtggag gtggtgaatg
     5821 atgctaagcg aggaaacgtc cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca
     5881 aaactggtat ggatgctgaa aggcagtccg ctcaccgtga ccggcgcagt catcattgta
     5941 ttaatgctac tgatgatgat tttttcaccg tggctggcga cgcatgatcc caacgccatt
     6001 gatttaaccg cccgcctttt gccgccttct gcggcgcact ggtttggcac cgatgaagtg
     6061 ggacgcgatc tgtttagccg cgtactggtc ggcagtcagc aatcaattct cgccggatta
     6121 gtggtggtcg ccattgcggg tatgattggt tcgctactcg gatgtctatc cggtgtgctt
     6181 ggcggacgcg cagacgccat catcatgcgc atcatggaca ttatgctgtc gattccttcg
     6241 ttggtactga caatggcact ggctgccgct ctcgggccga gtttatttaa cgccatgctg
     6301 gcgattgcta ttgtgcgaat tcccttttat gtgcgcctgg cgcgggggca agcattagtt
     6361 gtacgccagt atacctatgt tcaggcggcg aaaacctttg gcgcgtctcg ttggcatctg
     6421 atcaactggc atattttacg taactcccta ccgccgctga tcgtgcaggc atcgctggat
     6481 atcggtagcg cgattttaat ggccgccacg ttgggattta ttggcctggg tgctcaacaa
     6541 ccgagtgctg aatggggcgc gatggtagcg aatggtcgca actatgtact cgatcagtgg
     6601 tggtattgcg catttccggg ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt
     6661 ggtgatggta ttcgcgatct gcttgacccg aaagcaggag gaaagcagtc atgacccaac
     6721 ccgttctgga cattcaacag ctgcatttga gtttccccgg ttttaacggt gatgttcacg
     6781 cgctcaacaa tgtgtccttg cagattaacc gcggcgaaat tgtcggtctg gtgggagaat
     6841 ccggctcagg taaatcagtc accgcaatgc tgattatgcg tctgctaccg acgggcagtt
     6901 attgcgtaca tcgggggcag atttcactgc tgggagaaga tgttcttaat gctcgggaaa
     6961 aacagcttcg tcagtggcgc ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg
     7021 cccttaatcc gacacgtcga ataggtcttc agatgatgga cgtaatccgc catcatcaac
     7081 caataagtcg tcgggaagcc agagctaaag cgattgccct gctggaagag atgcaaatcc
     7141 cggatgccgt ggaagttatg tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc
     7201 gggtaatgat tgcgctggca ttctcctgcg agccgcaatt gattattgcc gacgaaccga
     7261 cgacggcgct ggacgtcaca gtacagttgc aggtactgcg tttgcttaaa cataaagcac
     7321 gcgccagtgg aactgcggta ctgttcatca gccatgatat ggccgtggtg tcgcaactgt
     7381 gcgatagcgt ttacgtgatg tatgccggaa gcgtaattga aagcggcggg acggcagacg
     7441 ttatccatca tccccggcat ccgtatacca ttggtttgct gcaatgcgca ccagaacatg
     7501 gagtaccacg ccagccatta cccgccattc cagggacggt accaaacctc acccatttgc
     7561 ctgacggctg cgcttttcgc gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc
     7621 cggcgctgac agcgtgtggt gacaacaacc agcactgcgc ctgttggtat ccgcagcagg
     7681 aggtcataag tgtctgacac gttattaacg ttacgcgacg tccatatcaa ttttccggcc
     7741 cgtaaaaact ggcttggtaa atctacggaa catgttcatg ccattaatgg tattgattta
     7801 cagatccgcc gtggtgaaac cttagggatc gtcggcgagt caggctgcgg caaaagcacc
     7861 ctcgcacagc ttttaatggg catgctgcaa ccgagccacg ggcagtacat ccgttcaggc
     7921 tcacaacgca ttatgcagat ggtgtttcag gacccgctct cttcgcttaa tccgcgctta
     7981 ccggtgtggc gcatcatcac agaaccgctc tggatagcta agcgtagtag tgaacaacag
     8041 cggcgagcgt tggcagagga gttggctgtg caggtgggta ttcgtccgga gtatctcgac
     8101 cgcctgcctc atgcgttctc cggcgggcag cggcaacgca tcgccattgc cagagcactc
     8161 tcttcgcagc ctgacgtgat tgtgcttgat gagccaacct cggcgctgga tatctccgtg
     8221 caggcgcaga tcctcaattt actggtaacg ctacaggaaa atcacgggct gacttatgtg
     8281 ctgatttcac acaatgtctc ggtgatacgt catatgagcg atcgggtggc ggtgatgtat
     8341 ctcgggcaga ttgtagaact gggcgacgcg caacaggtgt tgactgcttc ggcacatcca
     8401 tacacccgat tattgctgga ttccctcccc gccattgata aaccgctgga ggaagaatgg
     8461 gcattacgta aaacggatct gccgggaaac cgcacgttgc cgcaaggctg ttttttctac
     8521 gaacgttgcc ctctggcaac ccacgaatgt gaagtccggc aatcattagc aataagggag
     8581 gacggacgtg agctccggtg ctggcgggcg ctgtagagat gctaacaacg catcaggcat
     8641 tcacgcgcct gacgcgtcat ccggcaatgc tttacgattt caactggtaa tccagcgtaa
     8701 tttccgcttt cagtacctga gagaccgggc atcctgcttt tgctttctgg atgatgccgt
     8761 caaaggtaga ggcatcaata cccggcaccg caacttcact cttcagtgcg attttcgtaa
     8821 tcgcaaaacc ggcatccact ttatccagcg acacatcggc ggtggtatca attgatgttg
     8881 gcgtgaatcc cgcttccccc agcattaatg aaagcgccat tgagaaacat gcggcatgcg
     8941 ctgcgccaat cagttcttca gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc
     9001 catacggctg ttggttcagc acgccgctct cggtggatac tgttcccttc ccgcgtttga
     9061 tatcgccttc ccagtgtgcc tgacctttct tatggattgt cattgttgct ctcctgtggg
     9121 cttaaaaagg aaattataga ctacgtgaga aacagcacga ggataagcag gatattccga
     9181 ataaaatacg gcagaaaata ttacttctgc acttaaatta aattcccaca atagataacc
     9241 gggaatatat atctcaagag atattgcatc aatatcaatc aactcaccac aataataata
     9301 cctatcaata tcatggggtt aaaatataaa aatcatcaac taggactaat ctcaacaacg
     9361 aagtggtaaa tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac
     9421 cataaataca cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac
     9481 agcagaaccc gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt
     9541 cactgaagat gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat
     9601 cctcagtgat atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacgct
     9661 ttaaggtgct atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta
     9721 cggcgatgtt gtcc
//
LOCUS       DADLSE010000140         9596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLSE010000140 DADLSE010000000
VERSION     DADLSE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.141"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..931)
                     /locus_tag="JN692_003328"
     CDS             complement(<1..931)
                     /locus_tag="JN692_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HAY7265551.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQSEAGITRLLSSVEKSLDQAVQ"
     gene            complement(935..1498)
                     /locus_tag="JN692_003329"
     CDS             complement(935..1498)
                     /locus_tag="JN692_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265552.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETSWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGTAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(1473..1862)
                     /locus_tag="JN692_003330"
     CDS             complement(1473..1862)
                     /locus_tag="JN692_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HAY7265553.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            complement(1849..2403)
                     /locus_tag="JN692_003331"
     CDS             complement(1849..2403)
                     /locus_tag="JN692_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265554.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSERDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAESA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRASGESPGTELA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(2400..2807)
                     /locus_tag="JN692_003332"
     CDS             complement(2400..2807)
                     /locus_tag="JN692_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HAY7265555.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLTE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            2806..3039
                     /locus_tag="JN692_003333"
     CDS             2806..3039
                     /locus_tag="JN692_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265556.1"
                     /translation="MFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTV
                     GKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(3036..3977)
                     /locus_tag="JN692_003334"
     CDS             complement(3036..3977)
                     /locus_tag="JN692_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HAY7265557.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DISIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNQLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYGDGI"
     gene            complement(3989..4138)
                     /locus_tag="JN692_003335"
     CDS             complement(3989..4138)
                     /locus_tag="JN692_003335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265558.1"
                     /translation="MSSSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADGNAE
                     ISWKI"
     gene            complement(4122..4496)
                     /locus_tag="JN692_003336"
     CDS             complement(4122..4496)
                     /locus_tag="JN692_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265559.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRSYVTVN
                     LGSDFDASTIKKGDPVYVVVIH"
     gene            complement(4500..5720)
                     /locus_tag="JN692_003337"
     CDS             complement(4500..5720)
                     /locus_tag="JN692_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HAY7265560.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(5724..6467)
                     /locus_tag="JN692_003338"
     CDS             complement(5724..6467)
                     /locus_tag="JN692_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAY7265561.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQP
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(6358..7824)
                     /locus_tag="JN692_003339"
                     /pseudo
     CDS             complement(6358..7824)
                     /locus_tag="JN692_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            complement(7824..9227)
                     /locus_tag="JN692_003340"
     CDS             complement(7824..9227)
                     /locus_tag="JN692_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7265562.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINHDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            complement(9178..>9596)
                     /locus_tag="JN692_003341"
     CDS             complement(9178..>9596)
                     /locus_tag="JN692_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265563.1"
                     /translation="RRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
BASE COUNT         2168 a         2714 c         2334 g         2380 t
ORIGIN      
        1 tctgaacggc ctgatccagt gatttttcaa cacttgataa cagtcgggta ataccggctt
       61 cactctgggg tactttcgtg gtgctggtat aaagcaggtt atagaggttg gtctgcacat
      121 aattctgtaa ccagtccagg ccgtggcgtt catcaaagaa atcgccgtta gccatcactc
      181 cctgctggag gatagccgta tcattctggt agtacacgaa tacattgcag ttttttgcat
      241 caagtgccga tgcctggctg actgtcagtg tttcataccc gacacccggc tcctgcttaa
      301 acttgagcgt aatcgcggta ttactgccat tgaaattaac cgtgaatgcc cggccaaatg
      361 cagataacgc agcgtattta ttacccgatg aatactgaat aaaactgcgt gaatatccgg
      421 cggttttcag ttttgatgcc aaatcatcgc tggatgcagt ctgcaggcat ttctcatcgc
      481 ttgtcgtaat cgccagaata cggcttacag aagaggattc gatcgccgca gccactttca
      541 gccagtctgc atccggaata tctgcatcgt ctgcaatccc cagcccatac catgaagtat
      601 aatcaagcat ggcattcaca gcctgctcca gcgtctcagg cgtggcctgt tcgctgtctc
      661 ccttcgtttt cacccaacga ccaacaaaaa cctcctgagg tttcggtgat tgtgagaaaa
      721 acacctgcgc agccttatat tctggtgatt ccacgccaaa atcttttcca atatcttccg
      781 cagcagaata acggcgaatg cgctcactta ccggaatgat tgtggacggg ccgagaatga
      841 gtaatgcacc aaaatttcgc cctgatgctg cacgcggcga catgatcaca tcaacattaa
      901 caacgtttga tacaggcaag ccctgtgcca tagcttaatc tccgaaaaag atgactggtg
      961 cttccaccag cgatttaata ccgtactcgc gcacaacctt ccggcgcaga cgcaccgtca
     1021 tatcgtagcg gcggacccat tgctgattaa taagttcagg gaagggagtc agacctgtgt
     1081 aatcgccaag agacagcccc agcgcattca gtgctgcatt gttctgcggt acagatatac
     1141 cgtcacgaaa ccgggacgca tacaccatcc ccgccgtacc ataaaacgaa gccatacact
     1201 caatcgtttc atgccgccag agctgagagc catcatcggt ctgtctggtg aatgccggac
     1261 tgtcatcacc tgaccatccg ataaccccaa acgcacacca gctcgtttca accggtagca
     1321 gtggcggctg ctctttctgc cagcgcggac gaaccatccc ggcagacaga ccggaaacgt
     1381 tacgcatcca ctggcttaac agcctgtcga gcgcttcgtc ataatccgga tcgccactgg
     1441 ttggtatcag ccatccgcgc tctgtgctgg tgttattgct caaccggaat tcccccatca
     1501 aacggcagca actcacaatg cgcctgaacg aatccggcac catacgctgt atacgggtcg
     1561 acgaaagtca cacgataatc acggccctga tacgtcacga tatcggcatc acggccagtc
     1621 tgtccctgcg tcagtcgctc agtcgtcaca atcagaattg caccactgat tacctgcccg
     1681 gcctgcatac gacggttttc cagagagcga tcaacagtaa cgactccggc aaactgcttt
     1741 ttaacttcgc tgtcgctgcc gatcccatcc tcatccaccg tttgcacacg gcgtgttacc
     1801 cacaaattga agtcgcaaaa atcggggtca aaaagcacat ctgttacatc aagagtcggc
     1861 atctttatcc ctcacaacat gggtaatcgc tctgcgatat tgcccggtgt caattaatgg
     1921 tttcgccagt tcggttcccg gagattcgcc agaagcacgc cgggcaagtt ccagtgttgc
     1981 ccccttgcgc ccccgacgag cccgggcttc aacagtactg tcagcaagcg gcgtaaagcc
     2041 ggtaatggtc atgtaacgcc tgacgccatt agcggccagt gttccggcac ggttgagtgc
     2101 gctttctgct cccgcagcat taccatcaag tgcagcctgc gccgcggctt tgagctgcgg
     2161 caccgtctgc tcttctgccg atttaacgcc ggggaccaag tgaggtcgtg gcgggatgtt
     2221 ctgctctggt gagccgtatt cgttgaggta accgatgccc gcattaccaa acggaacatc
     2281 atcccgctcg ctgtcttccg aagggatgcc gaccagcaca tcttttttgg ttaacgacct
     2341 gagcgcatcc agaatggcct tagcgttatc caccctcgtt gttacaccgc ttttgaaact
     2401 catagctggc gaccgcctgc accgaacatc gtgatcaact gataaaattc agcgccatat
     2461 cgggtgttat tccagaaacc tgcatcagga ttcagcgtcg cgctggtgtc atagctgacg
     2521 cttaccttgt caacggactt tgaggactga acaccattgg ttgaaccgcc cggaccacca
     2581 gccagcatcg ctctgctgtc tgccgcccag agcgtcatgt agtgcgcaac gaacaatccg
     2641 gcaaagtacg gaaacaactt tttgccggtg acgttttcgg tcagcagttc atcggccaga
     2701 ttcagacgaa actggatttg cgcttcggga tatttggcag ggtcagcaaa ctgcgggaag
     2761 tcgcggcgaa aatcacttac cgctggcaga ctttgattct ttggcatttt ttacctcgtt
     2821 acgcgcgtct gtggctttgc caacggatac ttccgcgtgc gcacgagtga accagtgcgt
     2881 ggcaacgtct tcctccacaa catgacggcc tttaacaaac tcgcgccgag aaccgtcggg
     2941 aagcgtgagc acaaacgggg tatgtacgtg tattactgca tcattttttg ccatcgggtc
     3001 atccttaatg gccccgccag ggggccatgt ggctgttaaa tgccatcacc gtacgaaatg
     3061 gtttctttgt acactggctc gactgcaccc agcttgccgt agtaagtgac gatctgatac
     3121 agaccgcgat actgcaccgg cacgctctga agcggaacca gcgggtagcg gacgtatttt
     3181 ttatcgttgg tgtacgcaac catgcgatcc ttattcccca caccacggcc tttcagccat
     3241 ttaaccgcgc ggatattcag cggaacaccg ttctggtgat agctgatggt gttggtctga
     3301 aggtacgtca acagggactg gttacctgca gatgaaacga tgatgctgga caacagagca
     3361 aactgctcag gcgggatcag caaatcacgc ggaaccacag agtaaccaga agcggcccac
     3421 gcatcagaca gcacctggtt aatgcttgcg cggatttcgt ccggtgttga ggttgcccac
     3481 gttttggcag cgttgttgac aggcacgccg tccagggtaa caaggccttt caggtttaat
     3541 gcggaatcgc caacatatac ctgttcatcg ttatccatct gccatttcag ttgcataccg
     3601 tcatacttct gcgtatcaat cgggcggccg acctgctgag cagcctgcaa ttctatgacc
     3661 gtccagccaa gttccatccc ccacaggttc agctggttac cggatttgcc gatatccacg
     3721 ttcacgccag caatagcggt tgagtctttg cctacccagt tttttccatt cggatttgcg
     3781 ccagtacccg cagcggcgaa gctggtattc gtccagctgg aaatgtcatc tgcgatggag
     3841 atgtcttcac gcagttgaat atcgcggctc caggtgtacc ccaccagtgg caggttcagc
     3901 gtctggtcga gtcgctccag ctccccgatg agaaaggcac cagagctgtc aacggttgcc
     3961 tgatcaaaag taatcattcg tctgttcctt aaatcttcca ggaaatttct gcattgccgt
     4021 cagcatcacc ggcacctgtg aattcagcgt tggtcagcac cacgtttttg ccactgactg
     4081 acgtggacat gaatccaccc agcggcactt tgatggattc atcagtggat gacgacaacg
     4141 tataccggat cgcctttttt gatagtgctg gcatcaaaat cagaaccgag attaacggtc
     4201 acgtagctac gcttcatggc gtcgcccggg aagttcttgc ctgttcccac ctggcgaacc
     4261 atgtccggct gcgacgtggt cggataaggg cgcacgtaga tccccttcac cttgtctgcg
     4321 gtatcaccat ctgccagcgg cacgaaaaaa ccgtcatcat cgtatttacc agccaggcca
     4381 taggcagcga aggcgttatc ggatttaagg accaccggtt cgacggttaa gtcctgcggg
     4441 cgagagacag ccccggcaat gccaacaggc atccggtaca gaaatacatt attcattttt
     4501 taccctttac ggtttgccca gaattcagcg ttttgtttgt tcagggaagc gatactggtc
     4561 atgcccatgt ttaggcgctg tgcatcgccg gtggtggcgc gggtgtttcg ccctttggca
     4621 acctcagaca cggcattaaa cgccatgtcg accgattgtt tcggtaattt gcggatatcc
     4681 gcatcaccga ctatctggcg aaccagcgtt ttgtcagcag aagccagaac ctcgcgtttg
     4741 aacgcggtcg gtttcatctt acggctcaga tcgatacccg gaacgataac ttcggcacgc
     4801 caggctgagt caccagtaat cgtggtttcc tcttcatcgt cctcgccgtc accggtcgga
     4861 ttatcgtcag gcttattatc gttatcgccc gtggcatttc tttccagctt agccagcagg
     4921 gctttcagta atgttttgag gtcatcatca ctgtcgccgg ttggatctcc gcccatctct
     4981 ggtgctttgt ccggtagtgg ttgctgcggg gacaggttga tgttgagatt aacgccctgc
     5041 ggcaaatccc cctcatctcc tgtaaccgat gcgggagccg actccaccag ttcgttcatg
     5101 gtgtcggcat ctcctgtctt gatggccgca cgcatgcggt tccaccagtt tttcttttga
     5161 tttgccattg tgtctctgtc tccaattgca caacgatttc cggctctgcc tttagggaca
     5221 agagccacat ggtttccggt aatatcgacc tgctcggctt tacctggctc ggtctgctcg
     5281 tactccgcgt catagccgca cgacacttcg cgcaggccat cttcgataag ctgaatggcg
     5341 ctttcgtctt tgacgataag gtcagccagc atcaaatcag actgctcacc cgtcccgcgc
     5401 cggacattct ggaggtgccc gacagcaagc tctttccagt tctcgggatt taccagccgc
     5461 acattcccgt tttcatcttc aggatgcagg atcgtgatgc tcatcccttc gaatgaggca
     5521 agcgtagccg gatggaatac ctgctcagga gaacgcgtga cgactatttc accgaactta
     5581 tcgggtttca gttttggcag gtcatcagca ccatagagct gcttacctgt tcgtcctatc
     5641 ggcacgtctt tgcacagcaa cgagccgtca gccaggcggt atcgggtttc cccgagcctg
     5701 gtctcaaaaa agtatttcat gggttatgct gcccttttca ttgcgaatgg aacgcgaaca
     5761 aacacgatct ctttatagca gcggcagttt ggcagctcgc ccgcgtggcc ggtcatgccg
     5821 tccaaagttg gaggtgaatc ccagcggacg aatttacctt tcatgtgatc gtgggaatct
     5881 cggacatcgc catcatcggc tgtacgccaa atataaccat ccgagccaat agctatcgct
     5941 cgggccatat ccagcgcgcc cgttgctctt cccagttccg ttcgggcaat cagatcggcc
     6001 ctagacttag caacatcgcc ggtagatgca atctctttag caaactgctc cgcccgacca
     6061 ccggtgacaa cagcctctat cgctttgttc tggatgtcgt acaccctgtc tgcggcctcg
     6121 agaggcagag atttgatgta cttgacctgt tcggcgatga tgggttgcat cacctggccc
     6181 acaggagcgc tttccacaag attgcggagc tcgcgactga tgttcttgct gtgttgccgc
     6241 caaactttct cgttctgccg ggttaggtcc gcagtaaagt tttccgcgac ctttgtcgcc
     6301 cagggggtga tgatttcact gtaacgctcc agcgccgcaa taatttccgt gatactgtca
     6361 tttgaaccat cgtagcgacc atttacgatg tctccgaccg ccctcgctat cctgcgtagg
     6421 ctggttcgat accggatttc cgcctgacgg ctcctgcggt tcgtcatcag gttcgccgat
     6481 gccgggcggc gcttcatctt cggcattctc gatgtcctcg tcggtaatgg atgccccgat
     6541 gccggttacg tcagaatttt ctcgcaaatc agtcatagcg gctttcagtg tcatcagacc
     6601 atcacccagc gccgtactga ttgcgttggt ggtgtttaac gccaccgttg agcgatcgac
     6661 atcagacatt tgccagagcg ggttaaactc aaacgtgaaa tcgtccggca gcggcttgcc
     6721 aagttccgaa cgatgcatga tgtccagtat ccgccgcacc ggaagacgta aacgcctctc
     6781 ctgcaacgaa ctgatgcgat cgtaatagtt ggcaaggtct gcatcaccgg tagaaaatcc
     6841 tttcggggac tgtccgaaca accgcaccag tgggatacca acagcgccac taatctgttc
     6901 tgcaaactgc gaaaggatgt catccagacc actgaagctg tactgatggg tttcaaactt
     6961 atcccgcgag tccatgagcg tcatgccttc attactctgg aactgtcgaa tcaggtcgat
     7021 attcttcagc aacgcttcat acgcaggacc accaagtgcg ataagctcgc gtagcttctc
     7081 cacgctgtag gtacgcaaat gcgccttgta gaccagctgc gccgcgccga cagtagcgct
     7141 gtcgaacgcg gtaagacgat cccagatacg ctctacaacc gacattcccc attcgttctc
     7201 ggtcatcttc tgctgaaatg gcaacgtgac gccatcaaag cgaatcaggc gactgtgatg
     7261 aatgcgctag gcaggaattc ccgttgctgt ggtcaccaca tcgtaaaact caggtttacc
     7321 caggtccggc cccatatctt taatgcggcg ggtcagtgcc gggtcgacca tccagcggtc
     7381 gagcgggaga atccccttaa acttgccctt accgatggtt tcgggtcgca gcggggtcat
     7441 tggtgcctgc ccctcaatca tgatgaaacc caccgcgccg ccgtagaggc gcgaccattt
     7501 cagcacgtca ttcagcgcat cccagatttg caactcatcc agttgtgatt cgagaatgcc
     7561 acgatctttt gcatcaattt ccgaagtgat gcgaatgcct ttgcgggtca tatcatccgg
     7621 gatagcatcg actgcttcgc cgatgatcca ggatgaacga taggaccatt ccaccagcat
     7681 gcggttacga ctagtgaaat tagcccggta ggtggatgct gagtgctggt taggtgtctg
     7741 catccctacg cgggcaataa aattctcata accatcagct gtggcctgca cagttcgccg
     7801 cagggcttgt ttgtttcgtg ccatcaggcc tgtctcccca gctgttccca gatatccagc
     7861 gatgtatcga ttggcgcgaa ggccataatg aatgcatcag caacgtttgg tgacggtatc
     7921 tcgcgttttg cgaggtcttt tttactttcg accatcacac gtccgttacg gtcgaaatca
     7981 cgatgaggtg ttgtcagttc cagtttcagc ttttcaagca acggacaacg agaatctatg
     8041 ctgatcagct catccacagg atactgttct ccgttgttaa tggcgttaaa cgtatttctg
     8101 aaacggtcag ccaccagcca ccatgcctga gccttaagat ttgcgaaaaa gtctttgttg
     8161 gggatgccgt tgtactcgtc atctggctca tgcacaccag caccggcgtt aaacctctgg
     8221 taattcacac gtcgcgcgta tgcgttctcg ctcttccggt cagcgttaat ttcagagaat
     8281 ttagcaccgg cagacgcacc aacaccgata gagtcgtaaa caatatctgc ttcacgctcc
     8341 agcgccgcct gataagtacg ctggcagctc ttcagtaatt catcttcttt ggccttccat
     8401 tcgtcggccc agaaaacaac ggatccgtga cggtaaacgt tagcgcactt atctgtacca
     8461 ctgtcagcca cgtcaaagcc aatacgcttt cttccactgg gttcgaaatt taacgttttg
     8521 tgcgcatcca ctgcggcttc tatccaagac agtttgatga ttgccgcatc atcatcagac
     8581 tccggaacgc cctcatacac atgtttaaaa ccatccggat cacggcgtcg cgccgcgtcg
     8641 ataaccttaa gcatggtgtc agacagaaac ggattttcgt catggttaat tttgcggatg
     8701 agagtgcctt cgggcggatc aacaacgaag ttgcgccaga cgaaatcagt aacaagtccg
     8761 gggttgaata tgaaccagca ttccgaaccc tctttacgga tcgttggctc cagaattttc
     8821 cactggtatt ccgtcagtgc gtgggcttct tccagccaca acacatcgat cccctccagt
     8881 gacttaattt cttcgatgtt gcgccataat ccataaaaaa caaattccga accagtaacc
     8941 cggttaatga ttttgttgtt cagaatacgg aaacggtgcc gcagaccaaa cctgtctatc
     9001 tgaattttga gcagggtata caccgactct tcgattttat tctggatctg acgggcacaa
     9061 caaaaacgca gggtgtattt attcgacaga aatatggcaa tgccagcggc atcccatgat
     9121 tttgacgatg accgaccacc ataaagcact ttgttacgtg cccgcatagt ccagaaacta
     9181 cgtaaagccg gattcagcgt cggtttggat gtcagagtag aagtcattga ggtcacgctc
     9241 tccattgcca tcatcaatac ctgcatcacg gcgaagacga tcagcctcca gagacacctt
     9301 atcagtggcg gccttgcggt aggctgtatc agcaaatatt tttcctaccg tcgcaagcgt
     9361 gccaacgatg gactcaatac gaactgtatt gcgcatcatt gctttctcgg cggcgctgat
     9421 attttcaatc agcatcttcc tttcctggtc cccattagca tcttccagcg acaccaacca
     9481 ccgaccaata ttctctgcag cgacaaggtt gttagcacga aggcgaaata attcgtcctc
     9541 gagcgtcaac gctttagcgt cctctatcac ctcatctttg agcagaaggc gacggg
//
LOCUS       DADLSE010000141         9530 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLSE010000141 DADLSE010000000
VERSION     DADLSE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.142"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            20..157
                     /gene="sra"
                     /locus_tag="JN692_003342"
     CDS             20..157
                     /gene="sra"
                     /locus_tag="JN692_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HAY7265564.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            308..2005
                     /locus_tag="JN692_003343"
     CDS             308..2005
                     /locus_tag="JN692_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7265565.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHSPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAAKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2139..3149
                     /gene="adhP"
                     /locus_tag="JN692_003344"
     CDS             2139..3149
                     /gene="adhP"
                     /locus_tag="JN692_003344"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7265566.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3295..3579
                     /locus_tag="JN692_003345"
     CDS             3295..3579
                     /locus_tag="JN692_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7265567.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTSEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN692_003346"
     CDS             complement(3807..4460)
                     /gene="fdnI"
                     /locus_tag="JN692_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7265568.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN692_003347"
     CDS             complement(4453..5337)
                     /gene="fdxH"
                     /locus_tag="JN692_003347"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7265569.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN692_003348"
     CDS             complement(5350..8397)
                     /gene="fdnG"
                     /locus_tag="JN692_003348"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7810..7812),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7265570.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8629..9510
                     /gene="yddG"
                     /locus_tag="JN692_003349"
     CDS             8629..9510
                     /gene="yddG"
                     /locus_tag="JN692_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7265571.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGVHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSALLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2290 a         2514 c         2421 g         2305 t
ORIGIN      
        1 acaggattga ggagttaaaa tgaaatcgaa ccgtcaggca cgtcatattc ttggactgga
       61 ccataaaatt tctaaccagc gcaaaatagt taccgaaggc gacaaatcca gcgtggtaaa
      121 taacccaacc ggcagaaaac gccccgctga aaagtaattc ataaccatca gtcctcaatg
      181 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
      241 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      301 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      361 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      421 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      481 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      541 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      601 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      661 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      721 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      781 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      841 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      901 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      961 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
     1021 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
     1081 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
     1141 agtcggcaca ctgatcgcag ccagtcgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1201 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1261 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1321 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1381 gcgcgaaaac ctcagtgatt gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1441 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1501 agagatcatc cgtgagatgc ataaacactc tccgcgtccg atcgtgatgc cgctgtctaa
     1561 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1621 gctggtcgcc actggcagcc cgtttaatcc agtggtatgg aaagataaaa tctaccctat
     1681 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1741 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1801 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1861 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggccaa
     1921 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1981 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     2041 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     2101 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     2161 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2221 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2281 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2341 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2401 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2461 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc gccgattacg
     2521 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2581 ttaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2641 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcaa
     2701 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2761 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2821 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2881 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2941 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     3001 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     3061 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     3121 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3181 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3241 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3301 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3361 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3421 accggaaaag cagctttgac gtcagaaatg gcaattaaac tttccgtggt gatcggcagt
     3481 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3541 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3601 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3661 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3721 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3781 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3841 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3901 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3961 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     4021 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     4081 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     4141 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4201 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4261 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4321 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4381 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4441 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4501 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4561 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4621 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacat gcgtaccacc
     4681 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4741 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4801 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4861 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4921 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4981 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     5041 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     5101 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     5161 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5221 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5281 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5341 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5401 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5461 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5521 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5581 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5641 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5701 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5761 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5821 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5881 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5941 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     6001 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     6061 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     6121 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6181 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6241 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6301 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6361 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6421 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6481 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6541 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6601 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6661 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6721 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6781 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6841 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6901 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6961 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     7021 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     7081 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     7141 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7201 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7261 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7321 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7381 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7441 ttcgaaagca aaatcatcac gcaccagcag gctggcgttg gtgtaatgtt taacgtattc
     7501 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7561 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7621 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7681 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7741 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7801 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7861 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7921 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7981 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     8041 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     8101 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     8161 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8221 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8281 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8341 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8401 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8461 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8521 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8581 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8641 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8701 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8761 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8821 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8881 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8941 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     9001 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tggggtacat
     9061 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     9121 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9181 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9241 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9301 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9361 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9421 agcgccctgc tatcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9481 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg
//
LOCUS       DADLSE010000142         9519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLSE010000142 DADLSE010000000
VERSION     DADLSE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.143"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            58..1341
                     /locus_tag="JN692_003350"
     CDS             58..1341
                     /locus_tag="JN692_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265572.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAWIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1593..2510
                     /gene="oxyR"
                     /locus_tag="JN692_003351"
     CDS             1593..2510
                     /gene="oxyR"
                     /locus_tag="JN692_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7265573.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN692_003352"
     CDS             complement(2493..3893)
                     /gene="sthA"
                     /locus_tag="JN692_003352"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7265574.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            4214..4864
                     /gene="fabR"
                     /locus_tag="JN692_003353"
     CDS             4214..4864
                     /gene="fabR"
                     /locus_tag="JN692_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7265575.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4864..5223
                     /locus_tag="JN692_003354"
     CDS             4864..5223
                     /locus_tag="JN692_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7265576.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN692_003355"
     CDS             complement(5263..6363)
                     /gene="trmA"
                     /locus_tag="JN692_003355"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7265577.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6477..6667
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6732..8576
                     /gene="btuB"
                     /locus_tag="JN692_003356"
     CDS             6732..8576
                     /gene="btuB"
                     /locus_tag="JN692_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7265578.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8521..9378
                     /gene="murI"
                     /locus_tag="JN692_003357"
     CDS             8521..9378
                     /gene="murI"
                     /locus_tag="JN692_003357"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7265579.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLLA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2168 a         2291 c         2554 g         2506 t
ORIGIN      
        1 gcttaattat tttctgaaca gtcatcaaat cgattaattc cgctcaagga gctaaaaatg
       61 gacgttgatg tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag
      121 acagtgaccc tggttttatt atttatggcg tggatcgtta attttctcga ccgttcgtca
      181 ttgagcgtgg caggcgaagc gattcgtggc gagttaggat tatcggcgac agagtttggt
      241 gttttgcttt ctgcattttc tctgtcttat ggtttttcac aactaccttc cggtattttg
      301 ctagaccgct ttggcccacg aatagtttta ggcgcaggct taatattctg gtcattaatg
      361 caggcattaa caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg
      421 ggtattggcg aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgca
      481 caaaaagaac gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct
      541 atcgcgcctc ctgctcttgt attgatgcag ctggcatggg gctggcggac gatgttcgtt
      601 atcatcggcg tggcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg
      661 gcgcagtttg tcctgactga cgaagaacgg acgtatctct ccgcgccggt taaaccgcgt
      721 ccacagctgc aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg
      781 attttaggtt tctctggcgt caattatacc ggttggctct acattgcgtg gttacccggt
      841 tatttgcaag ccgaacaagg tttcagtctg gcgaaaaccg gttgggtagc ggcgattcct
      901 ttcctcgcgg cggcggtcgg gatgtgggtt aacggtattg tggtcgatcg gctggcgaaa
      961 aaaggctacg acctggcgaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg
     1021 gcattaggca cgttgctggt cgtgcaatct tcctcgccag ctcaggcggt agcgtttatc
     1081 tcaatggcgc tgttctgcgt gcatttcgcg ggaacgtctg catggggact ggtgcaggtg
     1141 atggtgtcag aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt
     1201 tttgcttcct ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat
     1261 ctggcgctgg ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt
     1321 gtcaaagatc gcattgagta atcgttacta tcaggctctc ttgctgtggg cctgtagaat
     1381 aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg
     1441 agaaactctc gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt
     1501 gagcaattat cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg
     1561 ccatgaacta tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg
     1621 tggcattggc tgaacaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc
     1681 cgacgcttag cggccaaatt cgtaagctgg aagatgagct gggcgtgatg ttgctggagc
     1741 ggaccagccg taaagtgttg ttcacccagg cgggaatgct gctggtggat caggcgcgta
     1801 ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt
     1861 ccggaccgct gcatattggt ttgattccta cggttggacc atacctgcta ccgcatatta
     1921 tcccgatgct gcaccagaca tttccaaagc tggaaatgta tctgcatgaa gcacagaccc
     1981 accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga
     2041 aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct
     2101 atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa
     2161 aactgctgat gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgctttg
     2221 aagccggggc ggatgaagat acacacttcc gcgcgaccag cctggaaact ctgcgcaaca
     2281 tggtggcggc aggtagcggg atcactttat tgccagcgct ggctgtgccg ccggagcgca
     2341 aacgcgatgg ggttgtttat ctgccatgca ttaagccgga accacgccgc actattggcc
     2401 tggtttatcg tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc
     2461 gcgcaagaat ggatggccat ttcgataaag tgttaaaaca ggcggtttaa accgtttaac
     2521 gcagctaccc gataggcttc cgccatcgtc gggtagttaa aggtggtgtt gacgaagtac
     2581 tcaatagtgt tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg
     2641 gcagcgcgct cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa
     2701 attttcagcg tgcccacgtt catgccgacg atttgtgcac gtgccagatg tttgaactgg
     2761 gcgcggccca cttcatacgg cactttcatc gcggtcagct gctgttcggt tttgcccaca
     2821 gagctgattt ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgtgcggtg
     2881 gcttcgcctt ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgctgatgcc
     2941 aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac
     3001 atgctgttga ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc
     3061 agcgaatcgg tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt
     3121 ttacccgact tcagatgcat gatcacaccg tcgtcacagc tttcgatctt ctcgtactct
     3181 tcgttgtgac gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc
     3241 tcttgatcga gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc
     3301 ataccgcgga agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca
     3361 tggcgcggtt cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg
     3421 aaatcaacat ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg
     3481 gttagtgttt caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag
     3541 cgagcgttcc cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc
     3601 gtttgttgat taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc
     3661 agtcgggaat ggtcgctgta tagtgggttt tgattgaatt ctataatgcg gctgacggcg
     3721 tgacggagag ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga
     3781 taacgctcga taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg
     3841 ccgccggggc cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg
     3901 cttacctgtt cttatacata aaagcaacag aatggtaaca ttctatcgcg ggtaagccaa
     3961 ttgatccccg tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac
     4021 ttttgccctg acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt
     4081 tccgctgtac gtaaaagaac cggccagtaa atatgtttta ttgcgttacc gttcattcac
     4141 aatactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
     4201 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
     4261 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
     4321 tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt
     4381 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
     4441 ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgtacctcgg tctccacatt
     4501 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
     4561 cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact
     4621 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
     4681 agcaatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca
     4741 acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta
     4801 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
     4861 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggctta
     4921 tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg
     4981 attatttccc tggtgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg
     5041 tcggtaggct tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc
     5101 acggtagttc gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc
     5161 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag
     5221 taatcggtat catgccggat gctggcgcat ccggcatggg ttttacttcg cggtcagtaa
     5281 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac
     5341 cttgtgcgtc tggcttaatg tttccagatt tttgcataac gtttccggat tgcaggagat
     5401 atacagaata cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg
     5461 cggtgggtcg acaaaaatgg tttcgaactg atagctcttt aagtcgatcc cttgcaggcg
     5521 gttaaactcg cgcacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
     5581 ttgtacgtta tcaatatggt tagcggcaat gttgtattgc gcggcagcga ctgacggttt
     5641 ggcgatttcg gtggctaata cccgatcaaa attgcgcgcc agcgctaatg aaaagttacc
     5701 gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
     5761 ttccagcatc tgaatattca tcgccgcgtt cggctgggta aagctgtttt ctacctgacg
     5821 atagatcatc tctttccctg cgaccggcag acgctcgtcg atgtaatctt gatccagcgc
     5881 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc
     5941 atcgcgcagt gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacagcag
     6001 ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg
     6061 cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc
     6121 actggcggcg gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat
     6181 gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcgcgcatcc ggtaatgact
     6241 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
     6301 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
     6361 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
     6421 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
     6481 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
     6541 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag
     6601 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg
     6661 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
     6721 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttccgtc acggcatttt
     6781 ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctatc cgttttgaac
     6841 agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct
     6901 ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa
     6961 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgc
     7021 tggtgttaat tgatggcgtc cgcctgaatc tagcgggggt gagtggttct gccgacctta
     7081 gccagttccc tattgcgctt gtccagcgta ttgaatatat ccgtggaccg cgctccgctg
     7141 tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg
     7201 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc
     7261 agcaacaact gggggataaa acacgggtaa cgctgttggg cgattatgcc catactcatg
     7321 gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt
     7381 ttttaagtaa aacgctttat ggggcgctgg agcataactt tactgatgcc tggagcggct
     7441 ttgttcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt
     7501 caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata
     7561 acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact
     7621 acgatcccca ttatggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca
     7681 ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact
     7741 ggcagaaaca gactacgacg ccgggtacag gttatgttga ggatggatat gatcaacgta
     7801 ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag
     7861 cacgcagcga cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt
     7921 gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa
     7981 atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac
     8041 agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc
     8101 gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag
     8161 ggaaagcgcg gattaagggc gtcgaggcga cagccaattt tgataccgga ccactgacac
     8221 atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac
     8281 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
     8341 gtattactta tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc
     8401 aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct
     8461 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct
     8521 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
     8581 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
     8641 cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat
     8701 ggcgaaaaaa gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa
     8761 gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt
     8821 cttgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
     8881 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa
     8941 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
     9001 ggctcggcag agatggttga gttggctgaa gcgaagctgc atggcgaaga tgtttctctg
     9061 gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt
     9121 gtattgggtt gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag
     9181 ggaacccggc tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa
     9241 catgaagccc cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg
     9301 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
     9361 ctggcagttt taggctgatt tggttgaatg ttgcgcggtc ggaaaattat tttaaatttc
     9421 ctcttgtcag gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa
     9481 acacgccgac gggtcagcgg ggttctcctg agaattccg
//
LOCUS       DADLSE010000143         9462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLSE010000143 DADLSE010000000
VERSION     DADLSE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.144"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            100..252
                     /locus_tag="JN692_003358"
     CDS             100..252
                     /locus_tag="JN692_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7265580.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            603..755
                     /locus_tag="JN692_003359"
     CDS             603..755
                     /locus_tag="JN692_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7265581.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(877..1497)
                     /gene="entD"
                     /locus_tag="JN692_003360"
     CDS             complement(877..1497)
                     /gene="entD"
                     /locus_tag="JN692_003360"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HAY7265582.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JN692_003361"
     CDS             complement(1654..3894)
                     /gene="fepA"
                     /locus_tag="JN692_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HAY7265583.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADARDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDMMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4137..5339
                     /gene="fes"
                     /locus_tag="JN692_003362"
     CDS             4137..5339
                     /gene="fes"
                     /locus_tag="JN692_003362"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HAY7265584.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5342..5560
                     /locus_tag="JN692_003363"
     CDS             5342..5560
                     /locus_tag="JN692_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HAY7265585.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5557..9438
                     /gene="entF"
                     /locus_tag="JN692_003364"
     CDS             5557..9438
                     /gene="entF"
                     /locus_tag="JN692_003364"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HAY7265586.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQWYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
BASE COUNT         2054 a         2488 c         2611 g         2309 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacac cctagggctg ccactccaaa ccagacaatt
       61 tggatggtag tcccttcttc gcatggaggc aatataaaca tgctgacgaa atatgccctt
      121 gtggcagtca tagtgctgtg tttgacggta ctgggattta cgcttctggt cggagactcg
      181 ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca gggctgttct cgcttacgaa
      241 ccgaagaagt agccgttgtg cggggagtaa tccccgcata tccggttgtc aggtcaggat
      301 ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga tggctgcggg cgatttcgtt
      361 ttaagagcaa tgatgagttt tgccgacaga agtccgaaac aggactcact gtataaataa
      421 acagctattt tgctaaggaa gggtaagata acggcgggtg cctgaagctt tccggtttca
      481 ggttaactct gaggtttgga aagatgaagc cccaagagat atttctatca atcctggggc
      541 tgccactccg aacctcaaca actcggatgg tagtcccttc ttcgcatgga ggcaatataa
      601 acatgctgac gaaatatgcc cttgtggcag tcatagtgct gtgtttgacg gtgctgggat
      661 ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt aaaggaacgt aatattgagt
      721 ttaaggctgt tctcgcttac gaaccgaaga agtagccgtt gtgcggggag taatccccgc
      781 atatccggtt gtcaggtcag agtggtaagg cacctgcttt acactttcgc ccgcagtcag
      841 tgatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc acagagttat
      901 gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac gatgaatgat
      961 gacctgctgt ttattccagc taattatctg atagtccaga aaacctgcat cagtttggat
     1021 ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgcc agtgtcagcg ccagagaaaa
     1081 ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt gtaataatgt tgtctgtcaa
     1141 ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca acgccaatcg gttgacggga
     1201 aaccacggcc aatgccgtag ccccgcagtg gctaatactg ccgtatacct ccgcaggcca
     1261 caccggttgg cgtagctcgc cgattgcggg cacacattta tagccatatt cccgcaaggc
     1321 ataaacagca gcgatccgtc cggctaaatg ctctgtttta cgtttacgtc cagcgtgttg
     1381 cagttgtgcg tagtgcggca gccagagtaa atcctgctca caaaaattcg ccggatcgaa
     1441 ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag ttttcatatc
     1501 gaccatattc gagactgatg acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc
     1561 tacgtgatcc tgcaatatat tgaattggca agatctttgt aggccgcatc cggcgtgaac
     1621 gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag tgagtattaa tgctcatata
     1681 ccacgtacgt cccggctcgt tataggtata cgcgccggca ccggcgatat agttggctcc
     1741 tgccagatcg cccgtggtct gggcattacc tgcacgccac aaacgtttgt cgaacaggtt
     1801 gtccacgccg ccggtcagac tgacattctt cgtcacatcc caggtcgcgc tcagacccac
     1861 aatgctgtaa ggactgattt ctttggtttc cgggccaacc gctggctgac ctttatagtt
     1921 gtacttcttc ggctgctgct tgccgtacca ggtgaaggtc gtttgcatcg acaaatcttc
     1981 ccgtgcctgc cagctcagcg ttgagttcaa cgtgtactcc gggatgatcg acaaacggtc
     2041 gcccgtggtt ttgttttcac tcttcagcat ataagtgatg ttattggtcc acatcaccgt
     2101 ttcgctaact ggtacgttta acgatccttc cagaccttca accactgctt tcggcacgtt
     2161 atcccactga tagagatcag tgccgactgc gttttgccct acagccacat agcccgcttc
     2221 aatcttattg cgataatcgt tacggaacca ggtgacgccc gccagccagc cgtcgcgttt
     2281 gaactccaga ccaatctctt tgttgatgct ggtttctgct ttcaggtcat cgttaccttg
     2341 cagatagcag ccgcccgcgc tggcatagca gccctgacct ttactgtaga gaatgtagtt
     2401 cgggttagtc tggtacaggc tcggcgcttt ataggcgcgg gcaatgccta ttttcagcgt
     2461 gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg ctccagttat tgccgacaat
     2521 actgtgatga tcgaaacgca gccccggcgt tacgatggtg ctgtcagtca gctccatgtt
     2581 gttttcggca aacagcgaga aaatttctgc ttttgaatac gggctacggt cggtggcact
     2641 cacgccatca atagcgccac cggtattcgt tccggtcagt gcctgggtgt tggaacttaa
     2701 gtccttcatc cgttgctgat tccactccgt acccagcgtc agcgtctggt taacgaggaa
     2761 atcaatcggc aggttaactt cgctgtgcag catcatgtca tcgagatcga tatcgacgaa
     2821 atcctgtgtc gctttttcgt taaatttccc ttcggtaccg cccgccagac cttccggaat
     2881 acgcgagtta cgggtgtgtt catactgcac ccagttgctg gtggtcacgc cgttatccca
     2941 gccaccgttc caggtcagcg cgtagttctg gcgatacagg cggttggttt catcgccata
     3001 tttcgagcga gtgtaagcgt cagagttggt gttctgggta tcgcccgcat acaggttacc
     3061 ctggcggctg taacccgctt ccagttccag cgattgcaat ggtgcgaagt cccagcgcac
     3121 cacgccgtta atatctttat tgatcacccc ttcgcgcccg gctggtaacg tcgtggcata
     3181 cgttccggca cgcgcggact gatggccctg gttgatatcc ctcgcgtcag cctgagtttt
     3241 gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc agcggaccgg tcaggctaaa
     3301 gttagtgcgt ttggtggcac cttcctcttt atgttctggc gcattgaaat atgcgtccca
     3361 ggagccgtgc cactcgccgc tgcctttttt ggtaatgatg ttaaccacgc cgcccgccgc
     3421 gccgttgcca taacgcgcag ctgccggacc acgcagaact tcaatacgtt caatcatttc
     3481 aggtggcacc caggaggtat caccacgggt atcgcgctcg ccacgccagc cctgacgcac
     3541 cgagttacgg ctgcttaccg gcttgccgtc aatcaaaatc agcgtgtttt ccggacccat
     3601 accgcgaata tcaatctgtc ggttattccc acgctgacca ctggtggagt taccggtcag
     3661 gttaacgcct ggcatggtac ggatgatctc cgacacatcg cgggcaaccg ggtttttgcg
     3721 gatttcatct gcggtgatgg tcgaaacgcc aggtgcctgc aagttctgct cggcggcggt
     3781 aacgacaatg gtatcgtcat gtgaaacagg agtatcggtc ggctcttgtg cctgcgctac
     3841 cccataaatc cccagattga ccaacaaggc cagggaatga atcttcttgt tcattgtttt
     3901 attcctgcat ttttgccacg aattgcaact gtcgggcatg gtcgtcatca acacgacgca
     3961 tcccgttacc gcgaaaacct ttgatcctga aagacacgca gtgcagttgg ttaattaatg
     4021 tccgcgcttc ccacggcgcg ccattacgct attgtaaatg caaatagtta tcaataatat
     4081 tatcaatata tttctgcaat caatgaaaaa ttgcacagta aacatggggt tatggtgtga
     4141 cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa acatggcccg gaatggcagc
     4201 gtctgaatga cgaaatgttt gaggtcactt tctggtggcg tgatccccaa ggttctgaag
     4261 aatactcgac gataaagcgc gtatgggtct acatcactgg tgtgaccgat catcatcaga
     4321 acagccagcc ccagtcgatg cagcgaattg caggtactga tgtctggcag tggacgacac
     4381 aactcaatgc caactggcgc ggcagctact gctttattcc caccgaacgc gatgacattt
     4441 tttctgcacc atcccccgat cgcctcgaat tacgtgaagg ctggcgaaaa ctattacccc
     4501 aggcaatagc cgatccgctg aatccacaaa gctggaaagg cgggcgaggg cacgctgttt
     4561 ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg ggattgtccg caagcgccag
     4621 aaataccagc caaagaaatt atctggaaaa gtgaacggtt gaaaaattca cgtcgtgtat
     4681 ggatttttac caccggcgat gtaacagcag aagaacgccc gctggcagtt ttgctcgatg
     4741 gcgaattttg ggcgcaaagt atgcccgtct ggcctgtgct gacttcgctg acccatcgtc
     4801 agcaacttcc tcccgccgtg tatgtgttga tcgacgctat cgacaccacg caccgcgccc
     4861 acgaactgcc gtgtaatgcg gatttctggc tggcagtaca gcaagagtta ttacccctgg
     4921 tgaaagttat tgcccctttt agcgatcgcg ccgatcgcac cgtggtcgcc gggcagagtt
     4981 ttggtgggct gtccgcgctg tatgccggac tgcactggcc tgaacgcttt ggctgtgtat
     5041 taagccagtc aggatcgtac tggtggccgc atcggggcgg gcagcaagag ggcgtgttac
     5101 ttgaaaagct aaaagctggt gaagttagcg ccgaaggtct gcgcattgtg ctggaagcgg
     5161 gtattcgcga gccgatgatc atgcgggcca atcaggcgct gtatgcgcaa ttacacccta
     5221 taaaagaatc cattttctgg cgtcaggttg acggcggaca tgatgcgctt tgttggcgcg
     5281 gtggcttgat gcaggggtta atcgacctct ggcaaccact tttccatgac aggagttgaa
     5341 tatggcattc agtaatccct tcgatgatcc acagggagcg ttttacattt tgcgcaatgc
     5401 gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta ccggcaggct gggacattgt
     5461 gtgtcagccg cagtcacaag agtcctgcca gcagtggctt gaagcccact ggcgtactct
     5521 gacaccggcg aatttcaccc agttgcagga ggcacaatga gccagcattt acctttggtc
     5581 gccgcacaac ccggcatctg gatggcagaa aaactgtcgg atttaccctc cgcctggagc
     5641 gtggcgcatt acgttgagtt aaccggagag gttgatgcgc cattactggc ccgcgcggtg
     5701 gttgccggat tagcgcaagc agatactttg cggatgcgtt ttacggaaga taacggcgaa
     5761 gtctggcaat gggtcgatga tgcgctgata ttcgaactgc cagaaattat cgacctgcga
     5821 accaatattg atccgcacgg tactgcgcag gcattaatgc tggcggattt gcaacaagat
     5881 ttgcgcgtcg atagcggtaa accactggtc tttcatcaac tgatacaggt ggcagataac
     5941 cgctggtact ggtatcagcg ttatcaccat ttactggtcg atggcttcag ttttccggcc
     6001 attacccgcc agatcgccaa catttactgc gcattgctgc gtggcgaacc aacgcctgct
     6061 tcgccgttta cgcctttcgc tgatgtagtg gaagagtacc agcaataccg cgaaagcgaa
     6121 gcctggcagc gtgatgcggc attctgggtg gaacagcgtc gccaactgcc accgcccgcg
     6181 tcactttctc cggcaccttt agcggggcgc agcgcctcgg cagatatttt gcgtctgaaa
     6241 atggaattta ccgacgggga attccgccag ctggctacgc aactttcagg tgtgcagcgt
     6301 accgatttag cccttgcgct gacggctttg tggctggggc gattgtgcaa tcgcatggac
     6361 tacgccgccg gatttatctt tatgcgtcga ctgggctcgg cggcgctgac ggctaccgga
     6421 cccgtgctca acgttttgcc gctgggtatt catattgcgg cgcaagaaac gctgccggaa
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcgcgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggatgcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagt ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggccctt attacctgct gggttattca cttggcggta cgctggcgca gggcattgcg
     9001 gcgcggctgc gtgcccgtgg cgaacaggtg gcatttcttg gtttgctgga tacctggccg
     9061 ccagagacgc aaaactggca ggaaaaagag gctaatggtc tggacccgga agtgctggcg
     9121 gagattaacc gcgaacgcga ggccttcctg gcggcacagc agggaagtac ttcaacggag
     9181 ttgtttacca ccattgaagg caactacgct gatgctgtgc gcctgctgac gactgctcat
     9241 agcgtaccgt ttgatggtaa agcgacgctg tttgttgctg aacgcacgct tcaggaaggt
     9301 atgagccccg aacgcgcttg gtcgccgtgg attgcggagc tggatatcta tcgtcaggat
     9361 tgtgcgcatg tggatattat ctctccaggg gcgtttgtaa aaattgggcc gattattcgc
     9421 gcaacgctaa acaggtaaat taatattatc tataaaccca ta
//
LOCUS       DADLSE010000144         9444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLSE010000144 DADLSE010000000
VERSION     DADLSE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.145"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN692_003365"
     CDS             complement(<1..810)
                     /gene="ugpC"
                     /locus_tag="JN692_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7265587.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLK"
     gene            complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN692_003366"
     CDS             complement(824..1483)
                     /gene="pgmB"
                     /locus_tag="JN692_003366"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7265588.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN692_003367"
     CDS             complement(1480..3747)
                     /gene="ycjT"
                     /locus_tag="JN692_003367"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7265589.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGYVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(3744..4799)
                     /locus_tag="JN692_003368"
     CDS             complement(3744..4799)
                     /locus_tag="JN692_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7265590.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCGSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAWEMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQLIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4809..5597)
                     /locus_tag="JN692_003369"
     CDS             complement(4809..5597)
                     /locus_tag="JN692_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAY7265591.1"
                     /translation="MKIGTQNQAFFPENIRERFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5615..6667)
                     /locus_tag="JN692_003370"
     CDS             complement(5615..6667)
                     /locus_tag="JN692_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAY7265592.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6698..7540)
                     /locus_tag="JN692_003371"
     CDS             complement(6698..7540)
                     /locus_tag="JN692_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7265593.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7527..8408)
                     /locus_tag="JN692_003372"
     CDS             complement(7527..8408)
                     /locus_tag="JN692_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7265594.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8429..>9444)
                     /locus_tag="JN692_003373"
     CDS             complement(8429..>9444)
                     /locus_tag="JN692_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAY7265595.1"
                     /translation="TSHDYAKVMDKEQLIDRKAVATVISNVGEGAFYDGVLRIVRTED
                     GSAWTGVPVSAWIGGIWYRKDVLAKAGLEEPKNRQQLLDVAQKLNDPANKKYGIALPT
                     AESVLTEQSFSQFALSNQANVFNAEGKITLDTPEMMQALTYYRDLAANTMPGSNDIME
                     VKDAFMNGTAPMAIYSTYILPAVIKEGDPKNVGFVVPTEKNSAVYGMLASLTITAGQK
                     TEAAEKFVTFMEQADNIADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLI
                     GELPNIQVFGAVGDKNFTRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELI
                     EQH"
BASE COUNT         2200 a         2677 c         2401 g         2166 t
ORIGIN      
        1 tttcagaacc gctaatttct cttcgggaat ggttaattta agcgtttccg taacgaattt
       61 atcgccatcg atcgtgccgc gaataaaatt catcgctggt gatccaataa atccggcaac
      121 aaacatattc gcgggttggt tataaacggt tttcggcgca cctacttgct gaacaatccc
      181 gtctttcata atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat cgtgggtcac
      241 gtagatcatg gtggtgttca gtttctgatg cagcttgctg atctctgcgc gcatttgtac
      301 gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg
      361 cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt
      421 gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat
      481 cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat
      541 atgcggatac aacgcgtagt tctggaacac catcgcgata ttgcgtgctt tggctggaac
      601 gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct caagcccggc
      661 aatcatgcgc agggtggtcg atttaccgca gcccgacggg ccgacaaata cgatgaactc
      721 tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat cgtagatttt
      781 ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg ttttgccaga
      841 aggccgataa ccgcgaccag gtcagggagt ccgttgaggg caacagtaat tgcgccccgg
      901 ttaagcccgc accgatcccc accgagcgca taccgctggc gttaatggcg tcaatgcccg
      961 cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg gcacaggcag
     1021 cgagaaagat ttccggatcc ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg
     1081 tgaaaaactc gcgcagctcc agcgccgcta aaatcgtcgg cgcattcagg gagacggaag
     1141 ccagccctac cggaatctgc tgtgcacgga gctctgccag caaattgcga ataccgggca
     1201 gcacagcatt aaccgtcagc tcgcgcagtg agtggacgta aagcagattt ttgcgatacg
     1261 ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc
     1321 gccgcagaga ctcatcgcgg ctgatccctt ttagagattc gttaaactgc gcatcaatac
     1381 tgatgccaat ttcagcggca atctgctgcc acgcctggaa atgcagatgc gcggtatcgg
     1441 tgattacacc atccagatcg aaaattaccc cttgcagttt cattcatcct cctgatgcgt
     1501 ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa cagaaaacag attcttcagc
     1561 cacggatata agctgaccgt tcaaacgcag tgaaacgggc gcagaagttc gaatcgcaat
     1621 acgctgcgcg tcgagagtga cctgtaattc gcagccctgc cagaacagag ggaaagacaa
     1681 ctgttgccac tgctcaggta acgccggatt tagatgcaat tcaccgtcac gcacgctcac
     1741 cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag catggatacc
     1801 atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc gccagaactg
     1861 ataactttgg gtcagcaggc cacagcgtgc agcaacaatg ccgtggattg ctttacttaa
     1921 tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac acgatgccga
     1981 tgagaactgc tctggcagca tgtaattgag catcaccaca tcagcttgtt tgaggatctg
     2041 catctcgttc acttctgcgc gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc
     2101 tttgtatttc gccagattaa tcgccggctt agtcataaac gaatcatcct gcggcaaaac
     2161 accgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg cacgatgaat
     2221 aaacgcgtcg tcactacagc caaactggcg ggcaatactc agcgcctgct ggacgttgta
     2281 atacaccata tagctggtga aggcgttatt attgacatgt tcggtatatt cgtctggccc
     2341 aataacatca tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa tccagaactt
     2401 tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg aaactttcat cccccgtggt
     2461 ctgccagtat tgaataaccg cccaggcgat atcggccacc agatgatgtt ccgcctgcgc
     2521 cgaggccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc
     2581 ttcttcgccg ctgcgcgcgc tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg
     2641 cgctttctcc tgcgcgcctg gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac
     2701 cgtcggatcg ctaaacagat ggaacggcag caaaaatact tctgtatccc agaaaacgta
     2761 gcctttgtag ccttcgccgg ttaagccttt tgccgcaata ctgctgcgct cgtcgtgagc
     2821 cggcgtcatg atacgcagat gataaagcgc ataatctaac gcttgctgat cgtgcgcatc
     2881 gccgccattt accgtgatac gacgtttctg ccaccattga cgccagtttt ctgttgatgc
     2941 tgcaagatgt tggtcgtaac tctgttgcgc gcacatttca agctggcgaa gcgacacgct
     3001 gccccactcg tctaaagcag cttgcctgtc atcccgccag tcgatccaca ccagtttttg
     3061 caacgtcact gtctcgcctg catgaagctg cgcactggta tgttgcagta aacggcgctc
     3121 tttggcggta tagcattgct gcacatcacc gctcacctgg cagcaacaac tgatggccac
     3181 atcactgcgc ccatcctggg tggtgtagat cccctgcatc aaatgctgac caaacacccg
     3241 cacctgggtt tcgtcgagat gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt
     3301 tgaaatcagc actgacgcgt cggcgtccag tggcgtaata gtgatttcca gcgcaatgag
     3361 cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg cgccgttgct
     3421 ggtacgccag acaacgctac ggcgtaattc tccactggca aagtcaagtt cacgctgcca
     3481 ggcttcgcgg gataacgaga aaacctcacc atttatggca atctccatcc ccaagatatc
     3541 aggcagattc accagttcgt tgatttcacc ttttcccgcc cgatgataca gccccgccag
     3601 atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata
     3661 gccgttcccc tgcgccatca gcgatgcata cttgttcagg gtatgctggc tgaaatgggg
     3721 ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc tgcggattga
     3781 tataacgccg ccaccagttg ctggatgatg tacccctgct cggcgtcggc tatcatcacg
     3841 ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg gttgtcgtct
     3901 gccatttccc gttgcatcag cgtcattaat tcaccgttgt tatcggtgta gatatgtgct
     3961 ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat caactgttcg
     4021 cggatgttga gtgcaaatga cgtttccagc cacagaatgc cgccgttatg aaattcaatg
     4081 gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc aaattgacca
     4141 cagctctttt gcgtgccgat cttttgaaaa ctatgcgcat tcacgctttt caccgccgga
     4201 aagcccagca catacatcgc agcatccagc atatgaatgc cgatgtcgat cagcgggcca
     4261 ccaccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg
     4321 gcgcgagcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg ttcacgcagc
     4381 tgttgcgtat cgagcgcaaa acgatggtga aagtcgtagg ccagcacctt gcccagtttg
     4441 cgcgcggtat cgcacatttc ccacgcctgt tctggcgtca tggcgggcgg tttttcgcac
     4501 atcacatggc agcctgcttc cagtgccatc agggtatgtt cgtaatgaaa acggttaggt
     4561 gagcagacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg atcgtcccac
     4621 acggatgcat tcccgtattt ttctgccagc gcctgcgcct gggaaaggcg gctgccacag
     4681 acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac cttatccgcc
     4741 acctggcccg cgcctattat cgcgacccgc agcggagagc ttgtcattgc acttttcaca
     4801 aagacctctt agcaggtacg caaccaggcc aacgaatcac ggtacgcctg ggcaggatct
     4861 tctgcccgga tacgcccttc atacaccaca taaccctgat agttatcggc gcgcagctgt
     4921 tcaaacagcg cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg gttatcggca
     4981 atatgcacat gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag gttatcttct
     5041 tcgatgttca tgtgatagaa atcgccgata atctgcacat gtttaagatt gttttcgacg
     5101 atgtaacgac gagcatcggc gagggtgttg atcatatgat cctgatagcg gtttaacggt
     5161 tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg cagggaatca
     5221 ctcaccattt tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg taagcggaag
     5281 gtaaacatgc cccacgctgc cggaacgacg atacctttac cccccacttc tgccagcgcc
     5341 tcgagaatgc gttcgatctg ctttaagcca ttaagacgac gctcttcgat aaagtcacca
     5401 atccagccgt catagccgcc gcaggcggtg gtcactggta aaccggtttc tttgatcgcc
     5461 gctttgactt cttcgatgtt gttgaccagc agtttgccgt caatctcaaa accatcgaag
     5521 cccatctctt tgatataacg aaatctctcg cggatatttt ccggaaagaa tgcctgattc
     5581 tgtgtgccga ttttcatttt ttcattcctt tgaattaaaa cgtaacgccc attttgatgc
     5641 tctgttccgg atgctgatcg acatactgca tgtagctttc cgggctgttg gcaaaggtca
     5701 ctaccgggtc gattaaatct tcgcaattga gataaccgtt catcagcagt tcccagcagg
     5761 tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggttcg ctgcacgcgc
     5821 gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta aaaccttcag
     5881 caaatggttt ggcaaacgcg acataggaga tggtgccgcc gtaggccagg ccgcgcagcg
     5941 ccgattgcag cgcgtcggcg taaccgctgg tttcgataat cacatcggcc ccctgcttgc
     6001 cggtcagcgt tttgatctct ttgcctacgt cagtgccaat ggggttaagg cagaaatcag
     6061 caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca attaccaccg
     6121 atgcgccagc gcgtttcgcc agttggatgg caatttgacc gatcgcgcca agccctacca
     6181 ccacgacaaa atcccctacg cgtacgttgg catcgcgcac gccgctcatg gcaaactgcg
     6241 ccgggtcgta acagacggcg tttttccagg agctgccttg cggcattttg cgcagcttgt
     6301 agttattcac tgcgttaatg atgaccgtct cggagagcgg gccgtagccg catacgctgt
     6361 cgcccaccgc gtagtctgta acgtcgctgc cgcactcgat aatgtcgcca accaccatgt
     6421 tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc atctgccgga cgcggggtga
     6481 acatctgcca ctcgccgtta aagtcttcat cgataaacgg gctggcggcg cggaagtcga
     6541 ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttcact tcattggcta
     6601 aaatcgcccg atcttcatac tcaaccagcg cagcaacacg cggtgctgtg gctactaact
     6661 ttttcatgta aaactccttg taaagaaact ggccgcttta gcccttcacg ccaccggcgg
     6721 tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc ggcaatgcgg
     6781 tcaccagcga ggccgccatc atccgtcccc agatgtaatc aggcgtactg aacagcgcgt
     6841 tcaggcccac tggtaaggtg aaattgctgg cgctggagag gaaaatcgac gcaaacagat
     6901 agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg gacatcgcca
     6961 ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc atcgccgctt
     7021 cttcgatctc atcagggatg gtgtcgaagt aacttttcag cataaacacg gcggtgggca
     7081 gggtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaata ccaagcgcgg
     7141 tgatgatttt gaacagaggc accaccagca aaatgccaga gaacatatac accgtgtaaa
     7201 agctggcgtt gatggtcatc cggcctttaa agcgcaggcg ggaaagcgcg taagctccga
     7261 gaatgccgag aaataccgcc accacggagg aaaccaccga caccaccatg ctgttacgga
     7321 agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc tccagcgtcc
     7381 actgttgcgg cagcagcgta ggatgcagcg atatcgcctc tttcgcgctt ttgaacgagg
     7441 tcatcagcat cacaaaaaac ggaaacagcg tgatgatgag aaacagcgcc agcccgcagt
     7501 aaaaaccgat acgactgagc gtgcgtttat ttgttgccat tgaggttcac ccgtttcctg
     7561 gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgagatcgc cgccgctttc
     7621 cctaagtcat taaaggcaaa ggcggttttg tagagataga caccaagaat atcgaccttg
     7681 gttgtcagca gataaacatc agcgaacatg tagaacatcc agatggtgcg cagggtcacc
     7741 accgtcgcca ggaccggcat aattgcgggc agcgtgacga tgcgaaaccg ctgccaggca
     7801 ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg caaaatcgcc
     7861 agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac caccagcaca
     7921 aagctactgc ccggattatc aaaccacagc ggtgcctgct catagagatg cagaagatcg
     7981 acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca ggcaaacacc
     8041 agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt tttgcgcagg
     8101 cggaactcgc ggttgaaaaa catcgccacg gccagcccga gcacggtgct acccgccacc
     8161 accagcgccg tataccagac cgtcatccac agcgagtgcc agaagccggg atcggagaga
     8221 atgcgcacat agttactcac cccaacaaac gttgactcga tgttgggatt gagcggcaga
     8281 cgtaaaaaac tgatttcgat attcgacacc atcggccacg ccaccagacc gcccagcagt
     8341 aataagctgg gcgcgagaag cagcagcgca aacggcatat cggaacgacc tgaaaaaagc
     8401 ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca tccagctttt
     8461 tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg ttatgcacca
     8521 tggagctcac cacaccagaa cccgtcacat cgcccatgcg ggtaaagttt ttatccccta
     8581 ctgcgccaaa gacctgaata tttggcagtt caccgattag ctgattcggt agttcaccca
     8641 gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct ttattcaccg
     8701 gcagcgcagc acctggcgac atcatcaccc agtcggcaat gttgtccgcc tgctccataa
     8761 aggtgacaaa tttttctgct gcttcggtct tttgtccggc ggtaatggtc agcgaggcca
     8821 acatgccgta gaccgcagag tttttctcgg ttggcaccac gaaaccgacg tttttcgggt
     8881 cgccttcttt aatcacagcc ggaaggatat aggtggagta aatcgccatc ggcgcggtgc
     8941 cgttcataaa ggcgtctttc acttccatga tgtcgttaga acccggcatg gtgttggcag
     9001 caaggtcgcg gtaataggtc agcgcctgca tcatctctgg tgtatcaagg gtgattttgc
     9061 cttcggcgtt aaagacgttg gcctggttgg ataacgcaaa ctgggaaaag gattgttccg
     9121 tcagcacgct ttctgccgta ggcagcgcaa taccgtattt tttattcgcc gggtcattca
     9181 gtttctgtgc aacgtccagc agctgttgcc tgtttttcgg ctcctccagc cccgcttttg
     9241 ccagcacatc tttgcgatac cagatgccgc caatccaggc gctgacagga acaccggtcc
     9301 atgcgctacc atcttcggta cgcacaatac gcagtacgcc atcgtaaaac gcgccttcac
     9361 caacgttgct gatgaccgtg gcaaccgctt tgcgatcgat aagctgctct ttatccataa
     9421 ctttggcgta gtcatggctg gttt
//
LOCUS       DADLSE010000145         9430 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLSE010000145 DADLSE010000000
VERSION     DADLSE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.146"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(233..991)
                     /locus_tag="JN692_003374"
     CDS             complement(233..991)
                     /locus_tag="JN692_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7265596.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELDEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(999..2102)
                     /locus_tag="JN692_003375"
     CDS             complement(999..2102)
                     /locus_tag="JN692_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7265597.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2112..3293)
                     /locus_tag="JN692_003376"
     CDS             complement(2112..3293)
                     /locus_tag="JN692_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7265598.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3361..4386)
                     /locus_tag="JN692_003377"
     CDS             complement(3361..4386)
                     /locus_tag="JN692_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7265599.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4817..5038)
                     /locus_tag="JN692_003378"
     CDS             complement(4817..5038)
                     /locus_tag="JN692_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265600.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5291..6675)
                     /locus_tag="JN692_003379"
                     /pseudo
     CDS             complement(5291..6675)
                     /locus_tag="JN692_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018912.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6678..8300)
                     /locus_tag="JN692_003380"
     CDS             complement(6678..8300)
                     /locus_tag="JN692_003380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7265601.1"
                     /translation="MPVALYPTIAPPAVSVSANYPGADAQTVQDTVTQVIEQNMNGID
                     NLMYMSSTSDSAGSVTITLTFQSGTDPDIAQVQVQNKLQLATPLLPQEVQQQGISVEK
                     SSSSYLMVAGFVSDNPDTTQDDISDYVASNVKDTLSRLNGVGDVQLFGAQYAMRIWLD
                     ADLLNKYKLTPVDVINQLKVQNDQIAAGQLGGTPALPGQQLNASIIAQTRLKNPEEFG
                     KVTLRVNSDGSVVRLKDVARVELGGENYNVIARINGKPAAGLGIKLATGANALDTAKA
                     IKAKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMR
                     ATLIPTIAVPVVLLGTFAILATFGYSINTLTMFGMVLAIGLLVDDAIVVVENVERVMM
                     EDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMA
                     LSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGST
                     GRYLLIYALIVAGMVVLFLRLPSYLKRIRVSF"
     gene            complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN692_003381"
                     /pseudo
     CDS             complement(8308..9375)
                     /gene="acrE"
                     /locus_tag="JN692_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
BASE COUNT         2514 a         2450 c         2253 g         2213 t
ORIGIN      
        1 catcaaataa aacaaaaggc ccagtcttcc gactgagcct tttgttttat ctgttgtttg
       61 tcggtgaaca ctctcccgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
      121 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag
      181 caaaaaggcc atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga
      241 ttacctgcga taaaaatgcc ctcgtacgct ctgatttagg atgcgcaaaa aattcatcag
      301 gtgtagcttg ttccactatc tccccacgat ccataaaaat tacccggtca gcgacggtac
      361 gtgcaaaccc catctcatgt gttacacaca acattgtcat acccgactgc gccagcccaa
      421 tcatcgtatc cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggttcatcaa
      481 acaacataat tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac
      541 cacctgaaat ctgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt
      601 aatgcatcgc cagagcttca gcctctttct taggcatctt gcgtacccaa atcggtgcca
      661 gggtacagtt ctgtaaaacg gtcagatgag ggaagagatt gaaatgctga aataccattc
      721 ccacttcctg cctgacgcgc tcaatattgc ggatatcttc atcaagttcg atgccatcta
      781 cgacaatccg tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt
      841 taccggaacc agaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta
      901 tatttttcaa aacatggaat tgtccatacc atttatggac gttttccagc gtaatcatcg
      961 cgttagcagg ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg
     1021 ttaaaacgtt tttccagatg ttggctataa cgtgacatgc tgaaacaaaa gatccagtag
     1081 atcagcgcgg cgaaaacata cccttccgtc gacataccca accaggcggg atcaacggtt
     1141 gcctgctgaa cgctactgaa aagatcgaac aagccgatga tgatcaccag gctggtatct
     1201 ttgaagagtg cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc
     1261 agaataacca gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat
     1321 tgccctttag gcagcgcctg taatccacct cgcacgactt ccgcaacata tgctgactga
     1381 aacaggatca cgccaaccag cgcgcggatc agtttgtcga tactggtgcc ttctgccata
     1441 aacaacggca gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag
     1501 aactcgataa aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaat
     1561 gccagtaaga tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt
     1621 agcgtcagcc cgccccattg ccgggtttca acccgctcaa gaccaaaaaa accgccatac
     1681 atcagccacc agacaatcag tgggtaaatc accgcccagg ccgcaatata gcgaccgcga
     1741 tgcgggagta ttttccagaa cattggtgcg atcgatacaa gcccaatcag taatgcgagg
     1801 ttaattcgcc agcgttggtc gtgtgggtaa agcccataca taaactgacc aaatcgttcg
     1861 tgaatgaaga cccaacaggc accggctttt gtgcagtcag cacgcgttga gccaacccaa
     1921 ttagcctgca aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg
     1981 cagccaatag tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc
     2041 atcgcgcggc ttgagttatg gctcgccggg cgcgagggct gagacagcaa tacttttgtc
     2101 atcatgactc cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc
     2161 gagatagtca ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct
     2221 tgccccgtct ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata
     2281 gcggcggcaa gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata
     2341 attactcgca gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaacccc
     2401 agtgaccgag ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc
     2461 tcggcgataa atgcagaggt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga
     2521 attaaaacca ttccgccgcg gaagttaaag ccttgtaggg ctgggacatc ccagtgtagt
     2581 gctgcgccaa aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc
     2641 ggccaggagc ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc
     2701 ccaacagaaa ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc
     2761 agctgcggtg acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc
     2821 acagcttggc gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc
     2881 agcagcgggg gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc
     2941 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     3001 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     3061 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     3121 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt gtgaaataac
     3181 caaccaacaa tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc
     3241 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3301 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3361 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3421 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3481 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3541 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3601 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3661 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3721 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3781 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3841 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3901 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3961 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     4021 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     4081 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     4141 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4201 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4261 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4321 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4381 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4441 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4501 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg
     4561 aaaatgaaac atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa
     4621 taatgattgt tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt
     4681 atttcgaaag cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc
     4741 agaatatagc aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac
     4801 tccgcgccct gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag
     4861 ccgctgcccc gccagcaata gcaccagccg tttcccagtt atcctgagtc gtgcctttca
     4921 tacaggtatt ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat
     4981 cgtgagcaca gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg
     5041 tgttacctca gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa
     5101 tgccatttta tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa
     5161 ttgtaaaaat agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa
     5221 tatcttatta atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta
     5281 tgtttctgat ttatccttta aagcaacggc gaatcaccac aaagaacacc ggtacgaaga
     5341 agattgccag caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct
     5401 gcgcgccact gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga
     5461 gagaggtcat caggatagga cgcagacgca tacgtactgc catcagtgtc gcttcaacaa
     5521 cacctttacc ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt
     5581 tggccgacaa gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat
     5641 taaagagtgt cgccgccagc agcacgccga caatccctaa cggcacgacc aacataaccg
     5701 agacaggaat tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg
     5761 aaattgctac cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg
     5821 tccagtcata accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg
     5881 catcgccgga actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt
     5941 tgtagcgttc cagtcgcgga gagccataca cccaatgtga agtggtaaag gcagagaatg
     6001 gcaccatttc gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac
     6061 ggaatttggc atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt
     6121 taacgtaagt cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac
     6181 ctaatgccgc gccttttcct ggtcaacttc cagtttaaac tgcgcggtgt cttccaggcc
     6241 attaggacgc acgctgacta agctggcagg atgttgcgcc gccataccaa gcaactggtt
     6301 acgggcctgg gttagggcat cgtgacccag cccagcctga tcaattaact caaagtcgaa
     6361 acccgttgcc gtgcccagtt caacaatggc tggcatattg aatggaatga caaaaccgtc
     6421 gcggatcttg cccaattcca ttttggcacg atggattacc gcttccgcac tgttttcatc
     6481 accattacgc tcttcccacg gtttcagact gacgaaggcc ataccggcgt tttgtgcctg
     6541 gccgctgaag ctaaagccgt taaccgtaaa gacactttca acgttcgctt tctcgttctt
     6601 cagatagtaa tccgtcactt gatccaacac tttttgcgtc cgctcttgcg tcgcgccagc
     6661 gggtaactga atcatggtca gaaagacacc ctgatcctct tcaggtaaga aggaagacgt
     6721 aaaaacaaca ccaccattcc tgcaacaatc agcgcataga tcagtaaata tcgtcctgtg
     6781 gaaccgagga ttttgccgac gctgttggtg tagtggttaa cgctatgatc gaaggtggta
     6841 ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct cagcagagac gggtttaagc
     6901 agcgttgcac ataacgcagg ggtaagaatc aatgccacca gaacagaaag tgccattgcc
     6961 gaaacgatgg tgatagagaa ctggcgataa attgccccag tagaaccgcc gaagaatgcc
     7021 atcggaataa ataccgctga cagcaccatc gcgataccca ccagtgcgcc ctgaatttgc
     7081 gacatcgatt tttccgtcgc ttcttttggc gggagcttat cctccatcat cacgcgctcg
     7141 acgttctcca ccaccactat cgcatcatcg acgagcagcc cgatggcaag caccatcccg
     7201 aacatcgtta gtgtgttgat ggagtaacca aaagtggcga ggatggcaaa cgtccctaac
     7261 aacaccacgg gtaccgcaat ggtggggatc agcgttgctc gcatattctg caagaacaga
     7321 tacatcacca ggaacaccag cataatggct tcgaacagcg tttttaccac ttcgtgaata
     7381 gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa ccttcattcc ctgcgggaag
     7441 aatggctgta attccgccag ttttgcctta atggctttcg cggtatcgag agcattcgcg
     7501 ccggttgcca gcttaatccc caggcccgcc gccggttttc cgttgatacg agcgataacg
     7561 ttatagtttt caccgccaag ttcaacccgt gcgacatctt tcaggcgtac caccgagccg
     7621 tcactgttta cgcgcagggt cactttgccg aattcttccg gattttttaa ccgcgtttga
     7681 gcaataatcg aggcattcaa ctgttgccct ggtaacgctg gcgttccgcc caactgtccg
     7741 gcagcgatct gatcgttctg taccttcaac tggttaatca catcaaccgg tgtcagttta
     7801 tatttgttta gcagatcggc gtccagccag atacgcatcg catactgtgc gccgaaaagc
     7861 tgtacgtcac cgacgccatt cagacggcta agcgtatctt taacgttaga ggccacatag
     7921 tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga caaagcccgc caccatcaaa
     7981 tagctgctac tggacttttc aacactgatc ccctgctgct gaacctcctg cggcagcaac
     8041 ggcgtggcga gctggagttt gttctgcacc tgcacttgcg cgatatcagg atcggtcccg
     8101 gactggaagg taagggtaat tgtcacgcta ccggcggaat cgctggtgga ggacatatac
     8161 atcaggttat cgataccatt catattctgt tcgataacct gcgtcaccgt atcctgcacg
     8221 gtctgcgcat cagcacctgg atagtttgcc gaaacagaaa ccgcaggcgg tgcaatggtt
     8281 ggatacagag cgacgggcaa ttgtaggatc gccagatttc agaccttcgc taatcaacca
     8341 tttatcgcca atcgcctgac tggcaacgac agggcgcgct tccacctgac ttttatcgtt
     8401 aacaatcagc acggttgcat caccacgcgg tgtgcggcta acgccttgtt gcgggataag
     8461 aatggcgtca ggttggacgc cttcatcaat ccgtgcacgc acaaacatac ccggcaaaag
     8521 cgtatgttgc gggttaggga agacagcacg tagggttatg gagccggtgc tttcatcaac
     8581 ggtcatatca gagaattgca gcgtaccttt caggggatag gtttgaccgt tttccatgac
     8641 caactctacg ttgctggtgg cgttttcctt atgcaaattt ccttgctcta cggattgctt
     8701 cagcctcata aaatcgttgc tggattgggt cacatcaacg tagataggat agagctgctg
     8761 gacagtcgcc agttcagtcg tttgcccatt agtgacaaga gcgccttcgg tcacggtcga
     8821 tttgccgata cgtccgctaa ttggcgcagt cactttggta taagcgagat tgatgcgagc
     8881 gctttcgact gtggcttttg cggcaatcac ggcggcatcg gcctggcgag catcagcaat
     8941 ggcctggtcg tactcctgct ggctgatgta tttcgtaccc acgagcggaa cgtaacgttt
     9001 taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc gccagttcgc ctttcgcgct
     9061 gtcataattt gcctgatagg tcgcgggatc gatctggtat agggactggc ctgcttgtac
     9121 atcgctgcct tcagtgaaat tacgattcag tacgatcccg ctaacctgtg ggcgaacttc
     9181 ggctatacgg taagcattgg tgcggcctgg taattcagtc ttaacttcta acggggccgt
     9241 tttcacaata tgaacggtaa cctgcggttc accgacgtga gctttctctt ctcccttatc
     9301 gttacaaccg gcgattaaag ccgcggagat cagaataaag gagggcagga ggaaaaacct
     9361 ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc gcgttattta cccagaaagg
     9421 caacgcgtta
//
LOCUS       DADLSE010000146         9299 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLSE010000146 DADLSE010000000
VERSION     DADLSE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.147"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(72..260)
                     /locus_tag="JN692_003382"
     CDS             complement(72..260)
                     /locus_tag="JN692_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7265602.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            complement(311..655)
                     /locus_tag="JN692_003383"
     CDS             complement(311..655)
                     /locus_tag="JN692_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265603.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            complement(795..2216)
                     /locus_tag="JN692_003384"
                     /pseudo
     CDS             complement(795..2216)
                     /locus_tag="JN692_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            complement(2240..2536)
                     /locus_tag="JN692_003385"
     CDS             complement(2240..2536)
                     /locus_tag="JN692_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HAY7265604.1"
                     /translation="MSIIECEIELDVINDSLAEFKCGNTLHSFIQISQKENVTVIIDD
                     GYVLGTFDCCVCAIKEIALLTARIEEADKKYGISYQRLKELSHIASSVRFFYAI"
     gene            complement(2611..2898)
                     /locus_tag="JN692_003386"
     CDS             complement(2611..2898)
                     /locus_tag="JN692_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265605.1"
                     /translation="MDMNPMLDYCFTPALRQTISHTGIKKWLWRIRKILQQKGDPDLI
                     QCDYHLCNKQFLPEKAFTGELIYMHGNMIARKKRKYCGAACAEKDKMAHEL"
     gene            complement(2909..3748)
                     /locus_tag="JN692_003387"
     CDS             complement(2909..3748)
                     /locus_tag="JN692_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7265606.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESLECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
     gene            complement(4170..4322)
                     /locus_tag="JN692_003388"
     CDS             complement(4170..4322)
                     /locus_tag="JN692_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HAY7265607.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(4515..4922)
                     /locus_tag="JN692_003389"
     CDS             complement(4515..4922)
                     /locus_tag="JN692_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7265608.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAGVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            5000..5227
                     /locus_tag="JN692_003390"
     CDS             5000..5227
                     /locus_tag="JN692_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7265609.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQK
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            5211..5633
                     /locus_tag="JN692_003391"
     CDS             5211..5633
                     /locus_tag="JN692_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265610.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGASDDSVVVH"
     gene            5724..6479
                     /locus_tag="JN692_003392"
     CDS             5724..6479
                     /locus_tag="JN692_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016060759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7265611.1"
                     /translation="MSMKLMAKAMEIKVGNPLRKLVLIKLADNASDQGECWPSYQYIA
                     DQCEISKRSVINHIAALCESGLVKKITRKGEKGNTSNIYLLHLDGAENLPGNSANNSL
                     HCASDASGSAGVAPVASAGVAPRTSHSFEPVNEPDVGGSADADPQVSHRAKNKIDWQR
                     VLDSYHEILPEMPSVKILTDTRKKNLRTFWQKFGFDQQRWESYLRYVAENCRWMLEDR
                     PNGSGGFWKRKNLDYLITERCYVAVKEARANDR"
     gene            6469..7872
                     /locus_tag="JN692_003393"
     CDS             6469..7872
                     /locus_tag="JN692_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7265612.1"
                     /translation="MTDNTFPVPYRVDAEQAVLGCLMVNTDTERAGLVYSMLKPESFY
                     VAAHRVIFREIRGLFRAGKPTDLLSLANVIEAKKLDAETGGFAYLAEISRSATLSAMV
                     HYAGIVREKSILRYAVEKLHACIGIMNQPTDTSATERLGAVQQVIGTVAEYARTGKTG
                     GLRPVSDVVNDWIDDLERRFSSPENAAGLTLGISSLDRLMAPKHVLRGSLVVIGARPK
                     MGKSASLNRIVTHFALNHRLPTLVFTLEMTDRSLVERMVAQEARVNSEIFYVGASDDS
                     DMARAMAKAAELAESNLMIDSTPGVTLAHVIAECRKVKRQRGVVGLVAIDYLTLMKAE
                     AAERRDIAFGDITTGLKNLAKELDCVVLLLTQLNRKLEDRADKRPTPSDSRDTGQIEQ
                     DCDVWIGLYRDAVYNRNADSQLMELILRLNREGATGTAYALMDNGSVTDVSAEDAARR
                     LRLGGPARKPYAQTEDF"
     gene            8039..8800
                     /locus_tag="JN692_003394"
     CDS             8039..8800
                     /locus_tag="JN692_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HAY7265613.1"
                     /translation="METVFDALKAMGKATSTELAARLDISREEVLNELWELKKAGFVD
                     KSAYTRRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPLFTETRADDLVLPSLHMANRELRRAKSH
                     VQKWECVCAALRELNKHRDIVRQITDISRRVASEK"
     gene            8816..9208
                     /locus_tag="JN692_003395"
     CDS             8816..9208
                     /locus_tag="JN692_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7265614.1"
                     /translation="MAKVFTPEEREEVKARIVELVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYDRRQN
                     IICRECRKSEVMQRVLAFYQGNFQEVMA"
     gene            9205..>9299
                     /locus_tag="JN692_003396"
     CDS             9205..>9299
                     /locus_tag="JN692_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7265615.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKR"
BASE COUNT         2366 a         2076 c         2403 g         2454 t
ORIGIN      
        1 tacagaatac tggcgggtgt ggttgatttt taataatcag ctatgaagtt ctaatctgga
       61 tataatgaaa ttcacgagga ctgaagtttc tcgcaattaa aatttatcag ttttactttc
      121 tgctctctgg aaacgcctgc ttctttttcc cctgagagca ttttttcgca ttctgatttc
      181 gttagttttg tctttgagta tcttgtccag ctggtaggag atccaccttc cttttcaata
      241 gtggctgtaa ttttatacat gaacacctcc attaatattt tcaaaaaccg tgtccaaaat
      301 gcacttggtt ttacaccgga atattctcat cgtatcctgc ttgttgcgtt tcgttttgtg
      361 tttgtggatc cgggaatgca tattcaacgc cttccagttc aatccatagt gcattacgac
      421 cagctttgat tgtcggccag tccatacctt tgattctttc ccatgagcgg gaggtgaatg
      481 ttttttccag caaatcagct tttgcgcgtt tggcgtcgtt actggtgccc ccatggtgct
      541 tgttgagcaa ctcgataatt tcatcaagtg cgatttcttt tgcacgcttt tcttttagcc
      601 atgtcggtgt accgtcgttg gcaaataatt cactgttatc ccgtgaggta tcgatgccga
      661 ggtgttcacc tcccaggtta agaaactcga tatgaggaag aaagttttta aattttgact
      721 tcatttgagg agatatgatg accgggaatc catttcgaat cattcgggtc gctaatccct
      781 tcaacaaatt ctccgcgaga ggcagccagt aatttgtctg catcgacagg atttttgggc
      841 ggaatgtttt tccgggcttc atggagttct gtccgcagtt tctgatattt cgcatcaaca
      901 gcatttacct gtgactgagc atccagcggc tgcgtgtcct gatgatgttc agttgcatcc
      961 ggttccactg tttcagccgt tgcctgttca tctgccattg cgcaagatgg ttgcggtttt
     1021 tcttcatcat cctgttttcc ttcttctgtt acacgttgcg gcatcggggc agaggagcga
     1081 ccgcaggcaa tatccacgat ttccggatca gggttggcat gatcggtttc agtcagtacc
     1141 ttgttcagat attcagtgac gtgcgcgggg atgacctcga taccaatcgg tgcatctttc
     1201 actgacgcaa ccacgatggc gcgggaataa tccatcccgc caggcatggt gatgaatttg
     1261 tcgcggaaaa cagaaaaggg cggtttattt tcagcgataa tttcctcgac acgtttagcg
     1321 tgtgccggat gaaggttata aatgtccacg tccattgaac gagccagtac gccagtggct
     1381 acgtcgcgcg ccagtgacgt cagatcgtga acgaaacctt cgccgcgatc ggtgaggttc
     1441 ccgccgccag cattagcacc ggaagccgtg cgagtgatac gcgaaacacg atttcctttc
     1501 atccactctt ttgtcagcag accgcgatcg gtgtagttgg catccagata tgcttcgaaa
     1561 aaagcagtca tcagtcccag atttgaattg ccaggattag ggaaaacttt gtcagtatca
     1621 cgcacgagtt tgtggaggtc gcgaatctcc agcgggtcga gcagactggt tttatgcgaa
     1681 acagccagag cagtaacagc cggtagttct tcatcccgag caatgtgtaa tgcctggagt
     1741 tcgtcgcgtg aaacgtgcgt taccggtttt tcgctgccgt gttgcgcaag ccaacgaatg
     1801 ggcagttcct gaccggaaac tggcaggagc atgttctcct caatttctgt catgtcttcg
     1861 ccgttgacgt tggtattgtc agtgctggct ggtttgtcct gaacagaggg agaagatgcg
     1921 ataaatacca ttgtgatgcc atcttccccg cctttttcgt aacggttgca gaattccgta
     1981 tcaaacacgc cttctggtgg gaggtcatca acaacgggca aattgacgcg aacaggtttt
     2041 ttaaagtcat cttcatcgta gccagcatcg tcaatcgcaa cagcaccacg ggagatggca
     2101 atggataatt ttttcgcttc agcccagtaa aaaccgcctt taataccgag gcgttttctt
     2161 actttgtcat tttttgcttc gtaatacagt gggtaaactt gtttatcggt gctcattgtt
     2221 ttttaacctc aactcatatt cagattgcat aaaagaaacg aactgatgat gcgatatgag
     2281 agagttcttt cagtcgttga taactgatac cgtatttctt gtcagcctct tcaatgcgag
     2341 ctgtgagtaa tgcaatttct ttaatggcac atacacaaca atcaaatgtt cccagaacgt
     2401 aaccgtcatc aataattacc gtgacgtttt ctttttggga aatctgaatg aaactatgta
     2461 atgtattccc acatttaaat tcggcgagag aatcgttaat aacatcaagt tcaatttcac
     2521 attcaataat gctcattgct gtttcctttt ttaaggttga gtgaatccct gccattgctg
     2581 gcataaattc agtttcgaat attcagttaa ttaaagttcg tgtgccatct tgtctttttc
     2641 ggcacaagct gcaccgcaat attttcgttt ctttctggca atcatgtttc catgcatata
     2701 aataagctcg ccagtaaaag ctttttcagg taaaaactgt ttattgcaga gatggtaatc
     2761 acattgtatt aaatccgggt cacctttttg ctggagaatt tttctgattc tccacagcca
     2821 ttttttaatc ccggtatgac tgatggtctg cctgagggca ggtgtaaaac aataatcaag
     2881 catggggttc atatccatag ctccattttt attttaagga aagcaactgc tctacggtca
     2941 tgtttttaat tgcgccccgg tttacaagag tccagccctg tttttccaga taaaaccgga
     3001 aagtctccag ggtacagacc agtgcgccat caggaacggt ttcggtgaat tcgacattgc
     3061 cgaatttgtc gaagtgaaca accagagtgc gaccatcacc cggaatcatc ttgtcagcag
     3121 gtggggtgtt attctggcgc agttcggcct ccatgcggtc gaactcagca atgtaggctt
     3181 ccttgaatgt ggcggctttt ttgcccgtga agcccatcac aaggaaaacg aagccgtttt
     3241 tggtgatttg gtaggcgtta taagaattgc cgcggtgttc gaatttaacc cgcgaaaagt
     3301 tgctggttaa aaatagttca gaacattcga gggattctat tttttgaaca acatggtgat
     3361 gctgcttgcc aaaaaactca gcaatcgaaa tagaagtagt gacggcgcga ccattttcga
     3421 tggttacgtc agggtgagaa agggtagtag ccataatgac atcctcaagt gataagttaa
     3481 ttaactcacc agcagaggtg ctaatctcat gggtggtgag acgcacaggg ttagcactac
     3541 cggtcactcg agaacccggc cagccttgcg gctgccccgc acgcctcacc ataatttgaa
     3601 tgtggctgtg cattacgcat aaaaaaaccg cttcagcgcg gttatgcgct cgagtaactt
     3661 tcggggtgct aatcccggca cccgttttat gaggtgcagg tgcactataa ttccacccgt
     3721 tctggttttc aatagctaca ttcaacattt tctctttcct ttcatcaccg aagtgaattt
     3781 tgtgatgcgg tgcctggtgc atccaggtga cgttaaccag ttaacaatta cagtcggctt
     3841 tcccacccaa aacaataagg actaacatga cttttaactg tgccgcgtgc gcttagccgc
     3901 attcaccgca tcacaaaatt cactttaaaa agggcggaca tcagccagca attaaaccga
     3961 tgccgccaac tggtacttca cacagcaatg tcgttattta caaccggaag cgcactccca
     4021 ccatttaaat ttcacagaca agaccgactc tttatgaata ccggaaatgc gccttcgtgt
     4081 tgtgcccggt tttatttcac cacctccggg ctttggtggc ctcggctata cctctacagc
     4141 gagaatattg aattaatcca ataaatggat tagctggtat ttttggcaag ccagcgacgt
     4201 gcgccagctt cggttttaaa cgttttgctt ttggtatacg tcatggcggt gaatgtgccg
     4261 tcctgattgg gaaacacgcc acacactagg gattcgttgt tgccaagatc gatagtatcc
     4321 atgctgacct catttcccct taacgccggg gtggcggaac tgtttactga gagcaccgtg
     4381 cggtgtcttg atagatgtta atttagtttt ctcatgataa ttgatcaagt atttttgatg
     4441 agaaatctca atatttaatg caaaataaag ccaatacatt gaaatgtaag gctttaaaat
     4501 ttgtgaaggg ggggttattg atgtttgtta cgtttgcgag cttctagtag ctcggtgaat
     4561 aggcgattaa aattctcaac gcgggcacgg agttcgctga tttgtgcttg ctgctctgat
     4621 tttggaagtg cgcgatacaa tcgcaacatc tccaactcat cttccgataa gtctaaggcg
     4681 ctgttgagtg caactggtgg atctggtgtt ttatcctcgt caccaaacag tatccaagtt
     4741 ggtgaacatt gcaatacccc agccaggcga tgcaaatttt gcccgcgcgg ggctgtatgg
     4801 tcgctttccc atagtgaaat tgatgagcca gatacgccag cagctttgct taaatcgttt
     4861 tgacttaaac caacctgttt gcgtctttct ctaattcgtt gacctaaagt tttctcgttc
     4921 atatttagat atcttaataa cccttgactt gagattcctt gagtgattac ttttgagaaa
     4981 actcaacttt ggaggggtga tgtttaaatc agacgtaatt aatttttatg ggacgaaagc
     5041 caaagtagcg aaagctgctg gtgttgatcc atctgctgtt tctcaatggg gtgaactggt
     5101 tcccgaaggt cgcgcgatgc gcctacaaaa agcatctggc ggggagcttc agtacgaccc
     5161 caaagtttat gacgaatatc gtaaggcaaa acgacctggg aaggtgattc atgaaaatca
     5221 ggcatgagca catcgaatca gtgctgttag ccctggcagc cgaaaaaggg caggcgtggg
     5281 tcgctaacgc aattactgaa gaatatctgc gccagggggg cggcgaattg cccctggtac
     5341 caggcaagga ctggaacaat cagcagaata tctatcaccg ttggttgaaa ggtgaaacga
     5401 aagcgcaaag ggaaaaaatt cagaaactga tccctgcggt tctggcaatt cttccgcgcg
     5461 agctgcgtca ccgactctgt atcttcgata ccctggaacg ccgtgcatta ctggcggcgc
     5521 aggaagcgtt gagtacggca attgatgcgc atgatgacgc agtccaggcc gtttaccgta
     5581 aagcgcattt cagcggtggt ggggcgtccg acgattctgt cgttgtgcat tgatgtttat
     5641 gccgaacctc ctctggttct gttgattggg gaatcacaga ttatatccgg aggaaggttc
     5701 ggcaccagac gaggtttcta tatatgagca tgaaactaat ggcaaaagca atggaaatta
     5761 aagtgggtaa tccattgcgt aagctggttc tgattaagct ggctgataat gccagcgatc
     5821 agggcgagtg ctggcccagc taccagtata tcgctgacca gtgcgagatt agcaagcgtt
     5881 ctgtgattaa tcatattgcg gcgctttgtg agtccgggct ggtaaaaaaa atcacccgga
     5941 aaggtgaaaa aggtaataca agtaatatct atctccttca tctggatggt gcagaaaatt
     6001 taccggggaa tagtgcaaat aattcgctac attgtgcatc agatgcatcg ggtagcgcag
     6061 gagttgcacc tgttgccagt gcaggagttg cacccagaac cagtcactct tttgaaccag
     6121 tcaatgaacc tgatgttggt ggatctgctg acgcagatcc acaggtcagt catcgcgcaa
     6181 aaaacaaaat cgactggcag agggttctgg acagctatca cgaaatcctg cctgaaatgc
     6241 catcagtgaa aattctgacg gataccagga aaaaaaatct gcggacgttc tggcagaaat
     6301 tcggttttga ccagcagcga tgggagtcct atctgcgata cgttgctgag aactgtcgct
     6361 ggatgctgga agatcgtccc aatggcagcg gtgggttctg gaagcgtaaa aacctggatt
     6421 acctgatcac cgaacggtgc tatgtcgctg tcaaagaggc gcgggcaaat gacagataac
     6481 acctttccgg tgccataccg ggtcgatgcc gaacaggcag tgctggggtg cctgatggta
     6541 aacaccgaca ctgagcgtgc ggggctggtg tattcgatgc tgaaaccgga gtcgttttac
     6601 gttgcggcgc acagggttat tttccgtgaa attcgcggac ttttccgtgc cggaaagcct
     6661 acggacctgc tcagccttgc gaacgtgatc gaggcgaaaa aactggatgc tgagactggt
     6721 ggctttgcgt accttgctga aatcagcagg agtgccacgc tatcggcgat ggttcactac
     6781 gccgggattg tgcgggaaaa atccatcctg cggtacgcgg tggaaaaatt acatgcgtgc
     6841 atcgggatca tgaatcagcc gacagacacg agtgcgacag aacgacttgg cgcagttcaa
     6901 caggtcattg gcacggtggc agagtatgcc agaacaggaa aaacaggcgg tctgcgcccg
     6961 gtaagtgatg tggttaacga ctggattgat gatcttgagc gtcgcttttc cagcccggag
     7021 aacgcagcgg gtctgacgct ggggattagc tctctggatc ggcttatggc ccccaaacat
     7081 gtgctacgtg gttcgctggt agtgattggt gcaaggccga aaatgggtaa aagcgcctcg
     7141 ctgaacagga ttgtgacgca ttttgctctg aatcatcgtc tgcccacact ggtattcacc
     7201 ctggaaatga cagatcgcag cctggttgag cgcatggttg cgcaggaggc gagggtaaat
     7261 tctgaaattt tttatgtcgg tgccagtgat gactcggata tggcgcgggc gatggcgaaa
     7321 gccgcagaac tggcagaatc aaacctgatg attgacagca caccaggggt aacgctggcc
     7381 catgtgatcg ctgaatgccg gaaggttaag cgtcagcgtg gtgttgttgg ccttgtggcg
     7441 atcgattacc tgacgctgat gaaggcggaa gccgcggagc gtcgtgatat tgctttcggg
     7501 gatatcacaa cgggcttaaa aaatctcgct aaagagctgg actgcgtggt tttacttctg
     7561 acgcaactta accgaaaact cgaggacagg gcagataaac gccccacacc aagcgacagt
     7621 cgcgacaccg ggcagattga acaggattgc gatgtgtgga ttgggttgta ccgggatgct
     7681 gtctacaacc gtaacgcaga ctctcagctt atggagctga ttctccgtct gaacagggag
     7741 ggagcaacag gaacagcata cgccctgatg gataacggtt cagtgactga tgtcagcgca
     7801 gaggatgctg ccagacgtct gagacttggt ggtccggcac gtaaacccta cgcacagacg
     7861 gaggatttct gatgcagtca ctttgtgatg ttctggaaaa aacgctgctt cagttcgggc
     7921 ggggggaaac ggcggagcag aaaacacgaa accagaagaa tctggttcat ctgagaaaaa
     7981 tgattgatgc agccaggctg gctgaaaagc tggaaaaaca aaaccggagg taattttaat
     8041 ggaaactgtt tttgacgcac tgaaagcaat gggaaaagcc acatccacag aacttgctgc
     8101 gcgacttgat atcagtcgtg aagaagtgct gaacgaacta tgggaactga aaaaggctgg
     8161 ttttgttgat aaaagcgcgt acacccggcg tgtggctgat aacaacgttc agcaggaaca
     8221 gccagcgcag gcagaactgc cggaagaaac caccacagca acagtagcga aaatctcaga
     8281 gtgcgattta accgcgacga ttgaacaacg cggaccacaa acggctgatg agctggctac
     8341 attgtttggt accacatcac gcaaagtggc ttcaacgctg gcaatggcaa tcagcaaagg
     8401 tcgtctgatt cgcgtaaatc agggcggtaa atttcgttac tgcataccgg gcgataattt
     8461 accagcagag ccgaaagcag catcggtagc ggaaactgat ggtaaggcct ttcctcagcc
     8521 cgcaggtgtt gcattaccag tacaggaggc tgcaacacag gaagatatta aaacagaaac
     8581 ggtggcggac attgtgcagt cgctgccatt gttcaccgaa acgcgagcgg atgacctggt
     8641 tttaccatcg ctgcatatgg caaaccgcga actgcgtcgg gcgaaaagtc atgtccagaa
     8701 gtgggagtgt gtctgcgccg cgctgcggga gctgaacaag caccgggata ttgttcgaca
     8761 gattactgat atttctcgcc gtgttgcatc ggaaaagtga ttgccggagg cacctatggc
     8821 aaaagtattt acaccagaag agcgggaaga agtgaaggcg cgcattgtgg aactcgtgcg
     8881 cctgagcgga cgagaaactt ttcgacaact ggcagataaa acgggtgtca gtaagaccgc
     8941 tattcgtcgt ttatctggtg cgcttgcggc cagtggtgat gtctggctct ctgattgcgg
     9001 ggtatttcca tcagagcagg cgtatcgcgt atggcgtaag acaccggaga aggctgctga
     9061 cccgacactg attcgaaagt tacctgacgg agaaatacgc cgctacgaca ggcgtcagaa
     9121 cataatctgt cgcgagtgcc ggaagagtga ggttatgcag cgagtgctgg cgttttatca
     9181 gggtaatttt caggaggtga tggcgtgagg gtgagagtct atatcgccgg tccaatgacc
     9241 gggtataaaa atttcaaccg tgaggcgttt cacaatgcgg aagaggaact gaaacggga
//
LOCUS       DADLSE010000147         9183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLSE010000147 DADLSE010000000
VERSION     DADLSE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.148"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..393)
                     /locus_tag="JN692_003397"
     CDS             complement(<1..393)
                     /locus_tag="JN692_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7265616.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAG"
     gene            complement(415..741)
                     /locus_tag="JN692_003398"
     CDS             complement(415..741)
                     /locus_tag="JN692_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7265617.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN692_003399"
     CDS             complement(960..1925)
                     /gene="glk"
                     /locus_tag="JN692_003399"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7265618.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            2129..3385
                     /locus_tag="JN692_003400"
     CDS             2129..3385
                     /locus_tag="JN692_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7265619.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            3500..3826
                     /locus_tag="JN692_003401"
     CDS             3500..3826
                     /locus_tag="JN692_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7265620.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN692_003402"
     CDS             complement(3967..5205)
                     /gene="mntH"
                     /locus_tag="JN692_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7265621.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            5541..6743
                     /gene="nupC"
                     /locus_tag="JN692_003403"
     CDS             5541..6743
                     /gene="nupC"
                     /locus_tag="JN692_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7265622.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(6793..8982)
                     /locus_tag="JN692_003404"
     CDS             complement(6793..8982)
                     /locus_tag="JN692_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HAY7265623.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGNLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         2373 a         2405 c         2197 g         2208 t
ORIGIN      
        1 accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag caaacatcgg
       61 aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa atttaagtaa
      121 ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc cgatctcagc
      181 aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca caccgcccat
      241 aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga gttcacccca
      301 gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg atgcgccagg
      361 cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg aatattactg
      421 ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt ttttaattgc
      481 gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat cttccggggt
      541 aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg tcaggcgatt
      601 ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt ctaccgccgc
      661 ttcttccagc gcctgagcgg ccataaaagt gtgagccagg cccattgggc aggcacataa
      721 ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac gagtgaaatt
      781 atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg gagaattaat
      841 cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag aaaaattgaa
      901 ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac aggaaggatt
      961 tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag acccggatag
     1021 tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg ccctttatct
     1081 tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg cacgataccg
     1141 cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc gccaaaacgg
     1201 cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct gtcagccagc
     1261 gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc agctttcaca
     1321 attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc cgaaacatga
     1381 ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt cggcgcaaaa
     1441 tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg gaccagatgc
     1501 gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc gaccggttct
     1561 gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat cgatacagcg
     1621 gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat ttcggcaatt
     1681 gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg gcaagcgatg
     1741 gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg aatgaccgct
     1801 tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc accactggca
     1861 atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa tgcatacttt
     1921 gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg taaatacctg
     1981 ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct gggtcacaca
     2041 attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg ggcaaactaa
     2101 gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa ccatgttgtt
     2161 attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga ttgtggtgat
     2221 gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa ctctggggat
     2281 agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta ttgcggtggg
     2341 gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg aaccgctgat
     2401 cggcgcaccg gttccgccct ctgcgctacc tggacttatc gtagcattaa ttctcggtct
     2461 tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata tcgcccttgc
     2521 cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga ctattttagc
     2581 ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt tgatattttc
     2641 gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg cgccgttaat
     2701 ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt tttcactgcc
     2761 cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa ttgtcgcggc
     2821 gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc acgcgatgat
     2881 gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc tcggaattct
     2941 gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc agcagatggt
     3001 ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta aacttgccgc
     3061 cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg cagtgtttgt
     3121 cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag cggcaataac
     3181 cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct
     3241 ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct gtatcgtcat
     3301 gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc gtccgaagca
     3361 acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt aagagttatg
     3421 gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct gacggcttcg
     3481 atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc tgatggcatt
     3541 taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca aattacaaat
     3601 tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag atcgtgatta
     3661 ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata atggctatca
     3721 tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct ggcgcgatcg
     3781 tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc gtcctccaat
     3841 ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac cggctcttta
     3901 acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga tgcgacgctc
     3961 attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg ccacgaccag
     4021 cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat cgcccatcaa
     4081 cttgctgtca ctggtgaaaa tcagcagagg aaccagcgcc agggcgatac caaaacttaa
     4141 cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa tgacaataaa
     4201 tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc gaatgaatcc
     4261 ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc cggcagcaac
     4321 cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca gcgtcagata
     4381 agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg ccgcagctgt
     4441 agccatcatc gccagattga caaaaccggc aatcgtcatg gcgatagcca catcccattt
     4501 ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga gcgaagagtg
     4561 caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca ggaagaccgc
     4621 ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca ggttaggctg
     4681 ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc cgccaatcac
     4741 tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag tcgcgatccc
     4801 cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac cgatcgccgc
     4861 accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa cccaatagaa
     4921 ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt taccggtggc
     4981 aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg cccaaacgac
     5041 aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg caaagttacc
     5101 gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga gcctcatctt
     5161 gcgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt gcctctaaaa
     5221 catagccttt gctatgtttc atgccatgcc aaacgagaat gattatcaaa ttcatttaaa
     5281 tggattgtgg tgatttctct gatagaccag gattatgatt acgaaaagat tgatgacttg
     5341 aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc catacacaac
     5401 atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg tcattatagt
     5461 gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga aatttaacct
     5521 gcctcatatt tggagcaaat atggaccgtg tccttcattt tgtactggca cttgccgttg
     5581 ttgctattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc cgttatgtta
     5641 ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc gacgttggtt
     5701 taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt gccaacgaag
     5761 ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc ttcctgaaag
     5821 tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac attcgcgtgt
     5881 tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt atgggcaaac
     5941 tggaatcctt taacgccgtc agctccctga ttctgggtca gtctgaaaac tttattgcct
     6001 ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct gccacggcaa
     6061 tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa ccgaaatacg
     6121 tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg ctgatcaatc
     6181 cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac gaaggtcaga
     6241 gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc attatcgttg
     6301 ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg tttgccaccg
     6361 ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac atcttctatc
     6421 cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc agtatcatgg
     6481 cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa attgcttcca
     6541 cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct ggtttccttc gctaacttct
     6601 cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa ggtaacgtgg
     6661 tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg ctgtctgcgt
     6721 caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca ttccccggct
     6781 tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc cttgcagata
     6841 ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa actccgcgac
     6901 cacactcaat gacttcgctt ttgccagatc ggtaattgat ctcacaatca tcgcatccag
     6961 cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat cagcctgcaa
     7021 acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg caatccggaa
     7081 accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag aaaacgcctg
     7141 ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct taaacagacg
     7201 aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat tgactgaaaa
     7261 gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg attccagcac
     7321 ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa acttatccgg
     7381 ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac cttcaccctc
     7441 tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg ccgtgcgaat
     7501 ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct ctctgctatc
     7561 cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta actgccccaa
     7621 caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac catagcccat
     7681 atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa ccatatgctg
     7741 gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac ccggcaattg
     7801 atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg agcggataca
     7861 gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat caatgcgcag
     7921 gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac gaaagttggg
     7981 taggagcgtt aacggatctg tcagcgcctg caaatgccag cggcgattaa gccattcact
     8041 gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca gcaccgcgag
     8101 aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctggt tgtaattcag
     8161 aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa gattaccgac
     8221 cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa agtcgttttc
     8281 ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc ttaaggtaaa
     8341 tgcgcgtttc tctttgccca acgacggagc gatatccctg cgccacaata tctgcgcaaa
     8401 gtggggactt acaatcatgc gagtgagata gtagaaaagc atgttgtaaa tcagcactgc
     8461 ggtgaaaagg cttagtaaat caacgaccgt gaaaatggca tccgcatcgc cgaaaaaggt
     8521 ggatatcttt agcgggaaat caaagaagct ccccacaaga tacatgctgc atttgatgcc
     8581 gatcggcgtc accaaaccaa gccagaacag acgttgccag acatatcggc tggtcaatcc
     8641 ataacgccaa cgcgtcccca gctgccagcg taatattgcg caggcacaca acacgacaaa
     8701 cgtctggctg ccgagcatga ccgcagtctg taaaaaagag agatgaaagt tccacagatt
     8761 ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc cagccaaaga taaacaagat
     8821 tgacagcatt acgcacagtg gcatccacgc cagaaagata taactggaat ggataatggc
     8881 aagtggcgaa ataaaacggg atagctgaat aagtaccacg gtgagcgtaa acgctagtgt
     8941 gaatatcttg atatttttta tcaggttatg ctccacaaac atgagtacac tactctcact
     9001 gatcggggtt ttcaggcaca caaataataa gtttattcgc atcgcgaagc aaacaggggc
     9061 cagcctgttg ccgatttttc atccaatgat gctttcacca ggcggcgcta acccggacgt
     9121 agactgtatt acaaaagcgg caaaaagcag agacaaaaaa cccccgcttt gcagcgaggg
     9181 ttg
//
LOCUS       DADLSE010000148         9105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.149, whole genome shotgun sequence.
ACCESSION   DADLSE010000148 DADLSE010000000
VERSION     DADLSE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.149"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            46..1104
                     /locus_tag="JN692_003405"
     CDS             46..1104
                     /locus_tag="JN692_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HAY7265624.1"
                     /translation="MEEDFRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVG
                     GSYHLAESYPPVHYIATDNYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYA
                     FRQLVAEEKYRGVVYQGLETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQ
                     VCEHLHIPVPEKLCVIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(1212..2036)
                     /locus_tag="JN692_003406"
     CDS             complement(1212..2036)
                     /locus_tag="JN692_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7265625.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            2356..4386
                     /gene="malS"
                     /locus_tag="JN692_003407"
     CDS             2356..4386
                     /gene="malS"
                     /locus_tag="JN692_003407"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7265626.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            4564..5817
                     /gene="avtA"
                     /locus_tag="JN692_003408"
     CDS             4564..5817
                     /gene="avtA"
                     /locus_tag="JN692_003408"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7265627.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(5968..6441)
                     /locus_tag="JN692_003409"
     CDS             complement(5968..6441)
                     /locus_tag="JN692_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7265628.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(6543..7391)
                     /gene="yiaJ"
                     /locus_tag="JN692_003410"
     CDS             complement(6543..7391)
                     /gene="yiaJ"
                     /locus_tag="JN692_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7265629.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            7592..8590
                     /gene="yiaK"
                     /locus_tag="JN692_003411"
     CDS             7592..8590
                     /gene="yiaK"
                     /locus_tag="JN692_003411"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7265630.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            8602..9069
                     /locus_tag="JN692_003412"
     CDS             8602..9069
                     /locus_tag="JN692_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7265631.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGQEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         2303 a         2319 c         2267 g         2216 t
ORIGIN      
        1 gtaggggaat atttacaggc atcacaatca gaatgggata ttttcattga agaagatttc
       61 cgcgcccgca ttgataaaat caaggactgg ttaggagatg tcgtcattgc cgacttcgac
      121 gacaaacaga tcgagcaagc gctggctgat gtcgacgtcc ccattgttgg ggttggcggc
      181 tcgtatcacc ttgcagaaag ttacccaccc gttcattaca ttgccaccga taactatgcg
      241 ctggttgaaa gcgcattttt gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat
      301 ggtcttccgg aatcaagcgg caaacgttgg gccactgaac gcgaatatgc atttcgtcag
      361 cttgtcgccg aagaaaagta tcgcggagtg gtttatcagg ggttagaaac cgcgccagag
      421 aactggcaac acgcgcaaaa tcggctggca gactggctac aaacgctgcc accgcaaacc
      481 gggattattg ccgttactga cgcccgagcg cggcatattc tgcaagtatg tgaacatcta
      541 catattcccg taccggaaaa attatgcgtg attggcatcg ataacgaaga actgacccgc
      601 tatctgtcgc gtgtcgccct ttcttcggtc gctcagggcg cgcggcaaat gggctatcag
      661 gcggcaaaac tgttgcatcg attattagat aaagaagaaa tgccgctaca gcgaattttg
      721 gtcccaccag ttcgcgtcat tgaacggcgc tcaacagatt atcgctctct gaccgatccc
      781 gccgttattc aggccatgca ttacattcgt aatcacgcct gtaaagggat taaagtggat
      841 caggtactgg atgcggtcgg gatctcgcgc tccaatcttg agaagcgttt taaagaagag
      901 gtgggtgaaa ccatccatgc aatgattcat gccgagaagc tggagaaagc gcgcagtctg
      961 ctgatttcaa ccaccttgtc gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg
     1021 caatatttct actctgtttt taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat
     1081 gtaaatagcg aggtcatgtt gtagcgatga tgagaattgt cggcgtcaca tcagggaact
     1141 cagcacagcc acctgatgcg agcaaaagcc ttatccgacc gacaaatcag ataaggctcg
     1201 aggaaatgaa attacatatg cgccgcgatt aaccgttggt tatcttggta cattgcgaac
     1261 aggtagttgt tataactctt ccctttggtc gagtagccct tcagcttgtg aatcatcgct
     1321 gtggcagtca cttcctgatc cgctttacgc agctgtgcac gcgatttacg gaacgaagag
     1381 taagccgggt gcgtattcag gttagtgaca taggcgctca ccgattcttt gacagaacta
     1441 aactgtgagt accctttcac tttacccggc gcattggtac aacgtccttt catgcatttc
     1501 atgccgaaca gattgttatt gttgcgcgcc agcttcgacg ttccccaacc gctttctgct
     1561 gcagccatcg tcgccaccat actggtaggg ataatgtcta cgcgttcaag caaggtattc
     1621 cacgggattt ttcgcgtatt accagaccac ttcaccttgt agcgtttggc gatgtctttc
     1681 agacgcgcac gctcagcagg tgaccattgg ccctgatact gttttgaaat cagccagtta
     1741 cgttctgcag taatggctgc attttggctg gtaatgtaag gcattacggt ccggagaaac
     1801 gcctttttcc ttggtgttcc ggaagggtat tttcgcaaat caggaagtga actactcttt
     1861 gcactattgc gagaatactc ttgtttactg cttacctgtt tattactagc tttagttaag
     1921 tgggactttt gactcgctgt tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc
     1981 agagtgagta acataagaat catcgcccca tatcgtcgta tgggagtcaa aatcatcagg
     2041 tctcctggtc ggatttaatc attccaacac cttatatttt tcacaaattt gagagttgaa
     2101 tctcaaatca tatcaaaaat agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa
     2161 ctatgtcacg agttaacgat tcagattggc gctaaatcgc agaaaatgtg ggggttatcg
     2221 caaaattcag ccgttttttg cgcgcgatcg ctcacccttg cttctcatcc tgtggactta
     2281 ccgctcaggg atgagttttg tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat
     2341 aaggactcat ccaccatgaa actcgccgcc tgttttctga cactccttcc tggcttcgcc
     2401 gttgccgcca gctggacttc tccggggttc cctgccttta gcgaacaggg aacgggaaca
     2461 tttgtcagcc acgcgcagtt gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa
     2521 cagtgctggc agcctgcaga tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt
     2581 agcaacacgc cgcctcaatg gcgattgttc agggacggca aatatacgct gcaaatagac
     2641 acccgctccg gtacgccaac attgatgatt tccatccaga acgccgccga accggtagca
     2701 aacctggtcc gtgaatgccc gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc
     2761 actttcccgg aaggagccgc cgtacgggat tattacagcc agcaaattgc gatagtgaag
     2821 aacggtcaaa taacgttaca acccgctgct accagcaacg gtttactcct gctggaacgg
     2881 gcagaaactg acacatccgc ccctttcgac tggcataacg ccacggttta ctttgtgctg
     2941 acagatcgtt tcgaaaacgg cgatcccagt aatgaccaga gttacggacg tcataaagac
     3001 ggtatggcgg aaattggcac ttttcacggc ggcgatttac gcggcctgac caacaaactg
     3061 gattacctcc agcagttggg cgttaatgct ttatggataa gcgccccatt tgagcaaatt
     3121 cacggctggg tcggcggcgg tacaaaaggc gatttcccgc attatgccta ccacggttat
     3181 tacacacagg actggacgaa tcttgatgcc aatatgggca acgaagccga tctacggacg
     3241 ctggttgata gcgcacatca gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac
     3301 accagctatg ccacgctggc ggatatgcag gagtatcagt ttggcgcgtt atatctttct
     3361 ggtgacgaag tgaaaaaaac gctgggtgaa cgctggagcg actggaaacc tgccgccggg
     3421 caaacctggc atagctttaa cgattacatt aatttcagcg acaaaacagg ctgggataaa
     3481 tggtggggaa aaaactggat cagaacggat atcggcgatt acgacaatcc tggattcgac
     3541 gatctcacta tgtcgctggc ctttttgccg gatatcaaaa ccgaatcaac taccgcttct
     3601 ggtctgccgg tgttctataa aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt
     3661 acacctcgcg attacttaac ccactggtta agtcagtggg tccgcgacta tgggattgat
     3721 ggttttcggg tcgataccgc caaacatgtt gagttgcccg cctggcagca actgaaaacc
     3781 gaagccagct ccgcgcttcg cgaatggaaa aaaactaacc ccgacaaagc attagatgac
     3841 aaacctttct ggatgaccgg tgaagcctgg ggccacggcg tgatgcaaag tgactactat
     3901 cgccacggct tcgatgcgat gatcaatttc gattatcagg agcaggcggc gaaagcagtc
     3961 gattgtctgg cgcagatgga tacgacctgg cagcaaatgg cggagaaatt gcagggtttc
     4021 aacgtgttga gctacctctc gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa
     4081 gcagcagagt tattactatt agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc
     4141 tcgcgtccgt tcggtcctac aggttctgat ccgctgcaag gtacacgttc ggatatgaac
     4201 tggcaggatg ttagcggtaa atctgccgcc aacgtcgcgc actggcagaa aatcagccag
     4261 ttccgcgccc gccatcccgc aattggcgcg ggcaaacaaa cgacactttc gctgaagcag
     4321 ggctacggct ttgttcgtga gcatggcgac gataaagtgc tggtcatctg ggctgggcaa
     4381 cagtgacttt tccggcttcc cgttcgtcag tacctcggga agccgccaac caggataaaa
     4441 tgtcagccct aatcagcgtt gcgggataaa gcaccgctca ctcttcaaca gaccgatttg
     4501 caccccagca aatgtagcgt tattgttacc ttccttgcta cagagttcga cagatatccc
     4561 gctatgacat tctccctttt tggtgacaaa tttacccgcc actccggcat tacgctgctg
     4621 atggaagatc tgaacgacgg tttacgcacg cctggcgcga ttatgctcgg cggcggtaat
     4681 ccggcgcaga tcccggaaat gcaggactac ttccagacgc tactgaccga catgctggaa
     4741 agtggcaaag cgactgatgc actgtgtaac tacgacggtc cacaggggaa aacggagcta
     4801 ctcacactgc ttgccggaat gctgcgcgag aagttgggtt gggatatcga accacagaat
     4861 attgcactaa caaacggcag ccagagcgcg tttttctact tatttaacct gtttgccgga
     4921 cgccgtgccg atggtcgggt caaaaaagtg ctgttcccgc tggcaccgga atacattggc
     4981 tacgctgacg ccggactgga agaagatctg tttgtctctg cgcgtccgaa tattgaactg
     5041 ctgccggaag gccagtttaa ataccacgtc gattttgagc atctgcatat tggcgaagaa
     5101 accgggatga tttgcgtctc ccggccgacg aatccaacag gcaatgtgat tactgacgaa
     5161 gagttgctga agcttgacgc gctggcgaat caacacggca ttccgctggt gattgataac
     5221 gcttatggcg tcccgttccc gggtatcatc ttcagtgaag cgcgcccgct atggaatccg
     5281 aatatcgtgc tgtgcatgag tctttccaag ctggggctgc ctggctcccg ctgcggcatt
     5341 atcatcgcca atgaaaaaat catcactgcc atcaccaata tgaacggcat tatcagcctg
     5401 gcacctggcg gtattggtcc ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg
     5461 cgcctgtctg aaacagtcat caaaccgttt tactaccagc gtgttcagga aactatcgcc
     5521 atcattcgcc gctatttacc ggaaaatcgc tgcctgattc ataaaccgga aggagccatt
     5581 ttcctctggc tatggtttaa ggatttgccc attacgaccg agcagctcta tcagcgcctg
     5641 aaagcacgcg gcgtgctgat ggtgccgggg cacaacttct tcccgggact ggataaaccg
     5701 tggccgcata cgcatcaatg tatgcgcatg aactacgtac cagagccgga gaaaattgag
     5761 gcgggggtga agattctggc ggaagagata gaaagagcct gggctgaaag tcactaaaaa
     5821 ttgcctgatg cgctacgctt atcaggccta catgatttct gcaatatatt gaatttggaa
     5881 gaatttgtag gccggataag gcgtttacgc cgcatctggc ataaacaaag cgcactattc
     5941 cggcgttgag aaacgccgga atacgtttca aacaggctgc tgctgtagcc gatgccgctg
     6001 cactttctcg acatccatgc actgcaacgc ctttgtcggg caggcttcaa cacaggccgg
     6061 ccccgtctcc cgatgccagc acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga
     6121 aacgacctcc atcgcaccaa acgggcaagc cagcatacag cttttacagc caatgcaacg
     6181 tgtttgttca acgaaaatat gcccatgttc gcggcttatc gcgtcaacag ggcagacatt
     6241 cgcgcacggt gcatcttcac actgatgaca ggctactggc gtggtccagc tgtggtcttt
     6301 aatgacacga atacgggaaa taaactcgtc tggtgacaac gcagcgcaat cctgattctc
     6361 ctgatgcgac actgcgcaag ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc
     6421 cgcaataata aaccggttca tgatcactcc tttgcactaa aacagcagag tgccagagag
     6481 cgcgtcccta ctgctttgat ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag
     6541 cgttatgtga tcgcgcccag gtcatccctg acagtaaatc ccagttcatt agaaatagcc
     6601 tgcgcggttt cacgcagtgg tttcaggaga tttttctctc ccacctgttt cagacgtgat
     6661 gtcgaaagcg aaatcgacac ggcgtacggc acccgcccat gaatatcaaa caccggaaca
     6721 gcaatacagg agacgccgag ttcgttttct tctctgtcca tcgccgctcc gctttcacga
     6781 atgtgcgcca gttcgtcgaa catcgcgggc agctcggtaa tggtattgcg ggttaacggc
     6841 tggatctcat gctgatggtt ttcccagtat gacttcacgt agtccgggtg accaaacgcc
     6901 atgtagatct tgcccattgc ggaacagtag agcggcatat gctggccaat ataggcacgg
     6961 gttcgcagca tcccggttgt gggttccagc ttataaatca aaatagcgtg atcgtcttcg
     7021 cggctggaga agttaatggt ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg
     7081 gcgatatgaa tgatattcag cgaagacagc gccttctgcc cgacggcaat aaatttggta
     7141 gtcaggcggt aactccctgc ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc
     7201 tgcaataagc gatggacggt actcttattt aaaccagcca gctccgaaag atgcgccaac
     7261 ggacaaccgt ttggatagtt gctcaaaatc tcaatcagca tcaacccgcg aaacagactc
     7321 tggcttccgg ctggacgctc tttttcctgc gccatctcgt tctctttttt tcccatcact
     7381 tctttcccca ttttgtcgcg tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc
     7441 gacagataat ttataaccac ttgattttta tgtcttttga aattcatcaa tcagattgct
     7501 ttgttaaaaa gtgatcgata tatttgaaat ctagtttcgt atattgaaat tttaagccaa
     7561 aaaagcgatc aaaaaaacaa ggaagcctgg gatgaaagtg acatttgagc agttaaaagc
     7621 agcctttaat cgggtcttaa tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc
     7681 agagatgttc gcccgcacca ccgaatccgg cgtttattct cacggcgtta atcgtttccc
     7741 tcgtttcatt caacaactgg aaaacggcga tatcattcct gatgcccaac ccaaacgtat
     7801 aaccagcctc ggcgcaattg aacagtggga tgcccagcgt tcaatcggca acctgacggc
     7861 gaaaaagatg atggatcgcg ccattgaact ggctgccgat cacggtattg gtctggtggc
     7921 acttcgtaat gccaaccact ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa
     7981 aggctatatt ggcatttgct ggaccaactc catcgccgta atgccgccgt ggggcgcaaa
     8041 agagtgtcgc atcggcacta acccgctgat cgtcgccatt ccttccacgc cgatcaccat
     8101 ggtcgatatg tcgatgtcga tgttctctta cggcatgtta gaagttaacc gcctggcagg
     8161 ccgtcagctc ccggtcgatg gtggctttga tgatgagggc aatttgacca aagaacctgg
     8221 cgttatcgag aagaatcgcc gcattttgcc gatgggctac tggaaaggtt ctggcatgtc
     8281 gattgtgctg gatatgatcg ctactctcct ttccgacggc gcatcggttg ccgaagtcac
     8341 ccaggacaac agcgacgaat acggcatttc acaaattttt attgccattg aagtggacaa
     8401 gcttatcgac ggtcccaccc gcgatgccaa gctgcaacgt atcatggatt acgttactac
     8461 cgctgaacgc gctgacgaaa accaggccat ccgcttaccc ggccacgaat ttactaccct
     8521 gctggccgaa aaccgccgta acggcatcac tgttgatgac agcgtgtggg ccaaaatcca
     8581 ggcgttatga ggagataagt catgattttt ggacatatcg cgcagcccaa tccgtgccgt
     8641 ttgcccgccg ccattgaaaa ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg
     8701 gagccgggcg ttgtcgaaat cgacggcaag aatatttatg cgcaaattat cgatttaacc
     8761 acacgcgaag cggtggaaaa ccgtccggaa gtgcatcgtc ggtatatcga tattcagttt
     8821 ctggcgtggg gtcaagagaa aatcggcatt gctattgata caggaaataa taaagtcagc
     8881 gaatcactat tagagcagcg cgatattatt ttttatcatg acagtgaaca tgaatcattt
     8941 atagaaatga taccgggcag ctacgctatt ttcttcccgc aggatgttca ccgaccaggt
     9001 tgtattctgc aaacagcctc tgagatacga aaaattgtgg ttaaagtcgc attaacagcg
     9061 ctgaattaat caaataatcg tctacgccag aacgactggg taatg
//
LOCUS       DADLSE010000149         8964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLSE010000149 DADLSE010000000
VERSION     DADLSE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.150"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN692_003413"
     CDS             complement(132..1733)
                     /gene="tap"
                     /locus_tag="JN692_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HAY7265632.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGILTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLERPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQESATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN692_003414"
     CDS             complement(1779..3440)
                     /gene="tar"
                     /locus_tag="JN692_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HAY7265633.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN692_003415"
     CDS             complement(3585..4088)
                     /gene="cheW"
                     /locus_tag="JN692_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HAY7265634.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN692_003416"
     CDS             complement(4109..6073)
                     /gene="cheA"
                     /locus_tag="JN692_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HAY7265635.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSTEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFALGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN692_003417"
     CDS             complement(6078..7004)
                     /gene="motB"
                     /locus_tag="JN692_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HAY7265636.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPELRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVRVPTMPSAEPR"
     gene            complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN692_003418"
     CDS             complement(7025..7888)
                     /gene="motA"
                     /locus_tag="JN692_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HAY7265637.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSGETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQ"
     gene            complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN692_003419"
     CDS             complement(8015..8593)
                     /gene="flhC"
                     /locus_tag="JN692_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HAY7265638.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN692_003420"
                     /pseudo
     CDS             complement(8596..8946)
                     /gene="flhD"
                     /locus_tag="JN692_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
BASE COUNT         2036 a         2474 c         2209 g         2245 t
ORIGIN      
        1 ctattgccag tacacctcag acttatccgc cgaaaatgcg cgtcggaaag cgccaggcgc
       61 tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt catagcgcct
      121 tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc atgtcgtgct
      181 acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc ggcctgattc
      241 gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggagttctg ctgagtcacc
      301 tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc cccgccgttg ttcttccgac
      361 gccgaagcaa tttctcccat aatgtcgttc acgcgggtca ccgaactgac aatatcggtc
      421 atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt gactgactct
      481 tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac ggctggcaag attgcgcact
      541 tcacctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc ttccactgcc
      601 gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct gataatgtcg
      661 ccaatttttt gcgagctggt ggcgctctcc tgcatggtgt gagtcatggt actgacctga
      721 acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca gttccgacgc ctgtcgtgcg
      781 ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc cgtttgtgcc
      841 agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc gacaatctcc
      901 gcgatcccaa tgtgcatctc atggcttccc ttacgcacat cactcaccgt cccacgcaaa
      961 gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg accatatacc
     1021 gcaatcggac gctccagatt acccgcagca atgctgtcaa aatggctacc gataatggcc
     1081 agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta gatcgccgca
     1141 acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg gctttgcgca
     1201 ctggcggctt ccagcacatg gttgatctcc agctgccagg cctcaaagtt aacgtcaaac
     1261 gcattctgtg atccctgcac cggcgcagtg aggaaatccg aaagttgatt actttcaagc
     1321 caggtcgcct gatgttcgag atcgttgtgc cagcgggcaa aacttttctc cgtctctttt
     1381 tgcaatgccc tgacgtgctc gttgcccgca gtcatcgcca taaaactttt aaacagcgtg
     1441 gtggattgcg tcagactggc gcgcgccgtc gtcatcaacg ttttaatgtc atccgccgga
     1501 tagctaagcg ctgtcagaat gcctgctttg ttcagcgcgg tactggcctg taacattacc
     1561 gcccgagttt gcgctaatgc cgcacgttgc tgattgctct gctcgccgtg attcagtcgt
     1621 tgcaaatcgt cgcgaaatgc ccaaaaagac atgccgttac tgccaatctg caagatcccg
     1681 cagagaatca aaattaaaaa cagcgtggtc gaaattcgaa tacgattaaa catcaacgct
     1741 ccccatcagg cggcaatgac cgcgttagta aaaactcgtc aaaatgtttc ccagtttgga
     1801 tcttgttcag taattcgcag tcgtggctgt gccggtggtt gctcactggc aggacgggat
     1861 ggtgtttgcg gtttattggt gagtgggctg gctgccagac ggaacgcgga aaccgcttgc
     1921 gttaaacgac tcgcctgttc ttccagcgca gctgcggcgg cagctgattc ctgcaccagc
     1981 gatgcgttct gttgcgtgac gcgatccatt tccgaaaccg ccaatgcgac ttgatcgatg
     2041 ccacggctct gttcatccga tgccgatgca atctcgccca taatgtcagt cacgcgagtg
     2101 acagcattga cgatattgtt cattgtttcc ccggcgcttt cgaccagcac cgaaccggta
     2161 tcaacgcgcg agacggagtc ttcaatgagg gctttgatct cttttgccgc ctgggcgctg
     2221 cgactggcaa gattacgcac ttcacccgcc accacggcaa aaccacggcc ctgttcaccc
     2281 gcacgcgcgg cttcaaccgc ggcattcagc gcgaggatat tagtctggaa ggcaataccg
     2341 tcgataacgc tgataatgtc ggcaattttc ttcgaactat cggcgatctc atgcatcgtt
     2401 ttcactacgc catccaccac tttgccgccg tgctgggcgg tgtcggaggc actttgcgcc
     2461 agttgcgagg cctggcgggc gttatcggcg ttttgcttca ctgtcgcggt gagctgctcc
     2521 atgctggcgg cagtttcttc cagcgcggat gcctgctgtt cagtacggga ggaaagatcg
     2581 gtgttgcccg ccgcaatttc acgggtaccg gcatagatgg catctgaacc ttcgcggaca
     2641 tgagtgacgg tgtcagtcaa agagcgttgc atatgtgaaa cgctctgcgc caggtcaccc
     2701 atttcactgc gcccgtcaat ggtcagggta ttcgccaggt taccaccggc gatttcgcga
     2761 atgtgagcaa taatttttgc cagcggagta agcaacatac ggcgaatgcc gtaccacgcc
     2821 accagcagaa tcaataccac caccagcgcg ataaccgcca gttgccactg ggcaaatcgg
     2881 taatcatctg cgttgtcagt gacgatatcg cgatacagtt tttcactgct gagggcgtac
     2941 tgagcaaacg cttcgcccat tgcattttgc attccctggg ttggctgagc gaaataagct
     3001 ccagtattgc cataatcaag ataatcaatc agttcagtta acgctgtgta atagttttta
     3061 tatttttcat caaaattacg gctggtagcg accatttcag gtaacggtgc catgcttttg
     3121 aattttttat aatgcgtcgc ggcctgggcc aatgttttcc tggcgctatc gagcaattca
     3181 actttggcgt tactttgttg attggaggaa tccatcatca tccgcaccgc tgaacgactc
     3241 aggttaatgc gcgtttgcag cattaaatcc caggttgacg tcagctcgcc ctgctgttcc
     3301 cgtaattgat tggaaaccac aaagctcttc tggctatggt gaagggaaga aaaaaacaaa
     3361 ctgccggaaa taagctgtaa cagtgcgaat acccccagca ccattaccaa cagcgtgact
     3421 acgcggatac ggttaatcat aaggcacctt cctgaaaaca agttgatctc gttatcggca
     3481 agaagggggg aaactttatt gctgatgcca cccgccgcga aattgaaata aaaaacccga
     3541 tgcgcagatc atcgggttca tttcaattga ggaaatcggg agaattacgc cacttctgac
     3601 gccgcgctat ctaacagcgc catctcttcg ctgttcagca gtttttcgat gttcaccaga
     3661 atcaacatac ggtcgcccag tgcgcccagt ccagtgagat attctgttga aagcgtcacg
     3721 gcaaactctg gtgccggacg aatttgctcc gccgtcaatg aaagcacgtc tgaaacgccg
     3781 tcaaccacga tgccgaccac ccgctgtccg agattcagga cgataactac cgtgttgtcg
     3841 ttatagtcca catccacctg gctgaactta attcgtaagt caacaatcgg cacaataacg
     3901 ccgcgcagat tcgtgacgcc tttgataaac gctggcgtgt tcgcaatccg tgttacctga
     3961 tcgtagccac ggatctcctg cactttcagg atatcaatac cgtactcttc atcaccaagg
     4021 gtaaatacca gaaattcctg gcctgacggc tcgctggcca gctttgttac attcgtcata
     4081 ccggtcatat tgttaccttt ttactcattc aggcggcggt gttcgccata cgttgttcgc
     4141 ggtttatcgc ctgcaaggcg gagacatcaa caatcagtgc cacgctgccg tcgccaagaa
     4201 tggtcgcagc agaaatgccg gggactttgc gatagttact ttcaaggttt ttaaccacaa
     4261 cctggtgttg accaattaat tgatccacca gcaaggcgta gcggcgaccg ccactttgta
     4321 agatcaccac aattccctgg gtggcttcgg ttttcgcgcc cgcgacgttg aacactttcc
     4381 acagttcgac gatgggcaga tattcacccc gcacttccag cacccgctcg ccgccggcca
     4441 gtggatggag atcggcttca cggggttgca gtgattccat aacagcattc agcggcagaa
     4501 tgaaaacttc atccgcaacg cgtacggaca tgccgtcgag gatggccagc gtcagcggca
     4561 gtaaaatgcg gatcgtagtg ccagtaccct gcttcgactg gatttcgaca tgaccgccca
     4621 tctcctggat attacgttta acgacgtcca tgccgacgcc gcgcccggag acgtcggtga
     4681 cctgctctgc cgtggagaag cctagtgcaa atatcagcat tgcaacttcg tcgtcgctca
     4741 tgttttcgct gacagtcaaa ccttgcgagg ccgcttttgc cagaattcgc tcacggttta
     4801 gccccgcccc atcgtcggtc acttcaatgc aaatgttgcc gccctgatgt tcggtagaca
     4861 gaattaaatt tccgacgctg tttttacctg cggcgagccg tttttctggc agttcaatac
     4921 cgtgatcgag gctattgcgt accaggtggg tcagcgggtc gataatgcgt tctatcaggc
     4981 tcttgtcgag ctcggtggaa ctgcccacca gcgtcagttc tacctgcttg ccgagttttc
     5041 ccgccagatc acgcaccagc cggggatagc gactaaagac atattccatc ggcatcatac
     5101 gaatcgacat cactgattcc tgcaaatcac gggcgttacg ttgtaactgc cccatgctgg
     5161 taatcaaatc accatgatta accgggtcca gttcgctgga acgctgggca agcatggact
     5221 gggtgataac cagctcgccg acgaggttaa ttaattgatc aaccttttct accgctacac
     5281 ggatgctggt ggattcactg ctgcgcgtcg ttttttcccg ctccacgcgg ccggttggcg
     5341 cttgttcggc tgccagttta agcacaggtg gggtagctat ttttggcgaa acttctactg
     5401 tttcaaaggt aatctgatcg gcttcaatca caaaacagag taccgctgtg atgtcatctt
     5461 cggcgatatc gcccggtaat attgccgaga gagaatccgc ccctttcacc acgtcattta
     5521 acgttgtcag atgccccagc tcttcttcca gcaggtcgac ttcgccggcc ttcaggcgcg
     5581 aaaggataat tcgtcgcggc aactgactgc gactttgctc atcttgcggt tcacttttgg
     5641 caaccacact taatcgggtc actgcggatg gcgtttcgcc tttcgcttct aatgccagtt
     5701 gacgcaaggc ctggcagata taatcgtagc tggcggcatc cggctcttgc gactgtttat
     5761 aagcgtcgag ctgttcttgc atgatgtcct tcgtttccaa aaacagattg ataatgtcgg
     5821 tgttgagttg catctcacct cgtctggctt catcgagcag gttttccatc agatgcgtgg
     5881 tttcctgcaa aacgctgaag ccaaaagttc ctgcccctcc tttgatcgag tgggcagccc
     5941 gaaagatggc attcaattgt tcggcatctg gcgcttccgg ctgcaaaacc agcagatgct
     6001 gctccatatc agccaacagt tcgtccgctt catcaaaaaa tgtctgataa aaatcgctta
     6061 tatcgatgct cacgctgtca cctcggttcg gctgatggca ttgtgggaac tctgacctgt
     6121 ggtgcaacct caggtttttc cagggcgctt actggctcat tctggctttc ggcgttttca
     6181 tgcaaaatgg cctgttcggc ttgtttgttc agtaccagca ggctgatgcg acggttgacg
     6241 gcatcatcag gtccgcgatc gcttaagcgc atcgttgccg ccatgccgac gacacgtaac
     6301 actttgccgc tatccaaccc tccgaccatc agttcgcggc gggatgcatt ggcccgatcg
     6361 gcagaaagct cccagttgct gtaacctttc tcaccgctgg cgtagggaaa atcatcggta
     6421 tgacctgaaa ggctaatacg gttgggaata ccgttcagca caggcgcgat ggcgcgcaga
     6481 atgtcgcgca tatagggttc gacatcggca ctgccggttc taaacatcgg gcgattctgg
     6541 ctatcgatga tctgaatacg tagaccttcc tggaccagat cgattttgag atggagacgt
     6601 aacgcccgca gttccggatc ggactctatc aactggtcga gatcaccccg caatttccgc
     6661 aatcgacttt gctccatgcg ttttttcagc tcttcgatgt tcggctgctt attcacttcc
     6721 ccctggcttt gggtgtaatc atcaccaccg ccgggaattg ggctttcact attggaaatg
     6781 cgatcgccgc ccgtaaccgc tgtcgccagt ggagtccgga agtactccgc aatctgaatc
     6841 aactcttttg ggctggagat ggagatcagc cacatcacca gaaaaaaggc catcatcgca
     6901 gtcataaagt cggcataagc aatcttccac gatccatgtg ctgccccgtg gcttttggct
     6961 ttgcgtcgtt tgacgacaat aatcggatgc gcttgattct tcatgcttcc tcggttgtcg
     7021 tctgttattg cggatttttc accgcacgca catgctcttc cagttcaata aacgacggac
     7081 gttcgctgga atagagcgtt ttgcgaccaa actcaacggc gataggcggt gcgtaaccgt
     7141 tcagattaga aagcagagtg actttgacgc actgcatcat tttgctggtt tcgccgcttt
     7201 tctgacgtaa aacactcgct agtggggaaa taaatccgta agccaataaa ataccgagga
     7261 aagtccccac catcgcatgt gcgataagcg cccccagctc ggcggcagga cgatcggctg
     7321 aacctaacgc gtgaacgccc cccattacag ccgcgacaat accaaacgcc ggaagtgagt
     7381 ccccgaccag cgccagactg ttcgccggga cttctgcctc gctttcgtgc gtctcaatct
     7441 cttcatccat cagggcttcg atttcgaagg tgttcatgtg accgctgata atcaggcgca
     7501 gataatcgac gataaaatca agcatgacgc tatccgcgag gatgcgtggg tagctggcga
     7561 agatctcgct ctcacgggga ttttcaatat cacgttccag cgaaaacatc cccatctgcc
     7621 gcgatttcgc catcaaccga taaagcagag ccagcagatc catatacatt gctttggtgt
     7681 atttggagcg acgaaacagc aacggcagcg ctttcagcgt gcctttaatc gctttgccat
     7741 tattgccgac gataaacgac ccaatccctg caccggcaat aatcaccagt tcagcgggtt
     7801 gatagagtgc tccaaggctt ccaccggtca tcaaataacc gccgaaaact gtaccgagaa
     7861 caaccaggta acctaataag ataagcacga catcatcctt ccactgttga ccatgacagg
     7921 atgttcagtc gtcaggcgtt aacgcgcgat tggggcaaaa aaaagcagcg gtacgtcgtt
     7981 accgctgctg gaatgttgcg ccacaccgta tcagttaaac agcctgtact ctctgttcat
     8041 ccagcagttg tgggataata tcggcaggat tctgggaaag tttacgtctt tttactgccc
     8101 gggatggcgg ttgacataag ctgcaggcaa agctgccaac aggctggtga gcgtgggtaa
     8161 taaaattgcc gccgcagcag ttgcagctgg aaagttgcag taatccactt tcaacaaacc
     8221 gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc ttcttctgct tgtgggcact
     8281 gttcaaggta taaacggtag gctttgatca ccgcatcgac gccattacac aaaccggttt
     8341 tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg aacgttttgt tcccaggtca
     8401 taaaccagtc ggttgaaaat ggcagcatgc ctttcggcgg tgggcttccg cgcagttctt
     8461 tataaagttt tatcaggcgt ccgcgactta actgtgtttc gctttccagc atctgcaaac
     8521 gagcgcccag ggtgatcaat tccattgcca gctgaatatc ccgcgcttcc tgaacaatgc
     8581 ttttttcact catgatcagg cccttttctt gcgcagcgct tcttcaggct gattaacatc
     8641 attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt tgctggagat cgtcaacgcg
     8701 ggaatcttgc gtcaactgag taatcgtctg gtggctgtca aaacggaagt gacaaaccag
     8761 ctgattggtt tctgccagct taaccatttg cggaagagtc agtgccgcta acgttgtcgc
     8821 catttcttca tttatgccga gacgaaacat agcggacgct ttgtcctaaa caatcagacg
     8881 ctgtgcaagt agtaaatatg acaagttgat gtcataaatg tgtttcagca actcggaggt
     8941 atgcattatt cccacccaga ataa
//
LOCUS       DADLSE010000150         8943 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLSE010000150 DADLSE010000000
VERSION     DADLSE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.151"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(123..1040)
                     /locus_tag="JN692_003421"
     CDS             complement(123..1040)
                     /locus_tag="JN692_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7265639.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVFMTR"
     gene            1100..1378
                     /locus_tag="JN692_003422"
     CDS             1100..1378
                     /locus_tag="JN692_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265640.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN692_003423"
     CDS             complement(1379..1906)
                     /gene="gadE"
                     /locus_tag="JN692_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7265641.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNILEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN692_003424"
     CDS             complement(2705..3277)
                     /gene="hdeD"
                     /locus_tag="JN692_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7265642.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3532..3864
                     /gene="hdeA"
                     /locus_tag="JN692_003425"
     CDS             3532..3864
                     /gene="hdeA"
                     /locus_tag="JN692_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7265643.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3980..4306
                     /gene="hdeB"
                     /locus_tag="JN692_003426"
     CDS             3980..4306
                     /gene="hdeB"
                     /locus_tag="JN692_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7265644.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4370..5016
                     /locus_tag="JN692_003427"
                     /pseudo
     CDS             4370..5016
                     /locus_tag="JN692_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5058..5588)
                     /locus_tag="JN692_003428"
     CDS             complement(5058..5588)
                     /locus_tag="JN692_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7265645.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNI
                     KRINELVRHQHIDYLV"
     gene            complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN692_003429"
     CDS             complement(5744..6310)
                     /gene="slp"
                     /locus_tag="JN692_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7265646.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6557..7780)
                     /locus_tag="JN692_003430"
     CDS             complement(6557..7780)
                     /locus_tag="JN692_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265647.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSENKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8361..>8943)
                     /locus_tag="JN692_003431"
     CDS             complement(8361..>8943)
                     /locus_tag="JN692_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7265648.1"
                     /translation="FERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIEN
                     PVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHA
                     GADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVI
                     LLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2597 a         1756 c         1853 g         2737 t
ORIGIN      
        1 ttatcgatat cgaagcattg agcattttta actcgtttta tcttgaatgc aatcggcgca
       61 taacttattg aatcccatca aatcccgtct ttttttaccc attcccggtt atcttgaata
      121 aattacctag tcataaatac attctggcgg ctaccttcat ccactaatgc cgtgacatac
      181 atgccaggca gcaagtcacc atttgggttg gggaaaatcg cccgtaacgt cacggagccc
      241 gtggtttcat caaccgtcgg gtcggagaat ttcagcgtgc cggtctggct gtaacgttta
      301 ccattttcca gattgagctg tactggcgta ctgccctgaa cctgtttgat ttgcccactg
      361 gcgacctctt ctttcatgcg taagaaatct tgcaccgact gcgtgagatc gacataaatc
      421 gggtccagac gttgtacggt aaccagcgaa tctgcctgat tagcggtaac gagtgcgccg
      481 acggtcaccg acgatttccc gctgacgccc gtaatcggcg aggtgacatt cgcgtattgc
      541 agattgatcg tcgcctgttc aacagccgct ttggcgacgg tgacattggc ttctgcttca
      601 ttcagctggg tgcgcgcggt gtcgtaatcc tgacggctaa cgtagttggt cttcagcaac
      661 gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg tagagagcgc tttcgccagc
      721 gagcctttgg cggagtttag ctcggcctgt aaaggtgcag gatcaatctg atacagcgaa
      781 tcgccctggt tcactttatc gccttcgata aagttgcgtt taatgataat accgcccacc
      841 tggggacgta tctcggcaac ttcataagga acggttctac cgggcaattc gctcaacaca
      901 ttgaccgaac caggggagag tgtgacgaca ccgacctcag gcgtcatggc ggcggcgttt
      961 tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc agaataacaa cggtattaac
     1021 agctttcttc ttctgttcat tttagtccct gaaaattctt gagaaataac ggttctttaa
     1081 attgcgcgcg aggagagata tgagggattt acagaccagc ggcatagtgg ggttgtcagc
     1141 cagtaaggtg ggatacaggc acagtgatcg acatggtgag gtcaacgaca aaaaaaatgt
     1201 tgccgttctg ccaacagttc ctgccagcat tcgggcaacg aagggttcta ctggtggata
     1261 cacataccag gggaatcaag tcgttttagc ctctccttta gtaatcaccg ggggtaatga
     1321 aatcagcatc tgcatccctc gtcatgccag ccatcaattt cagttgctta tgtcctgact
     1381 aaaaataaga tgtgataccc agggtgacga tgtcgctcat acgtcttagt ttcagcttgt
     1441 acataatgtt gaatttctga atctttaatg tttgttgcga aatattgaga gtcgacgtga
     1501 tattgctttc atttttattt ttcagaatca gttgcataat tttgaactga ttatgtgaaa
     1561 aacataattg tgaattctta tggggcaagt gtttaccata agtaatctca agaatatttt
     1621 tgatactttc tttgcggcta acaaaataaa taggcgcttt agcttttagt ttactgatgt
     1681 gataaggtgc caaaacaata attctctcgg catctaattt ctccagaaat ttaatcgctt
     1741 cttcatcaag gatatgattg tgataggtat caatgataac cttgaagggt ttattgccta
     1801 cttttttgat ctctgacagg gaattgattt ttatcatttc gtgattatct ttcaactgcc
     1861 aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa aatcataact tgctccttag
     1921 ccgttatcgt ttcgaacatg tcatcctttt cattatttac atccttgtcc gaatcgttgt
     1981 tcaatatagt aaacgcctat aagaactttg cccttcgtga actaatacgt ctattaacac
     2041 taatcaatac ttcagattta tccatcacgc actccatata ttgatcctaa gtaagattcc
     2101 tggttgttat cagcttgtaa atagtaaaca aaaagttaac gtttgcggat tactgcccaa
     2161 gaataagtta tcaataacca gtttgtgatc tctgaagaat attactaaag ttaaaatgta
     2221 atccgattta aatatcgagt ctccttgttt cgacttaagc tggcaattgg attgccagct
     2281 ttcttttaac gcattgaaat tacctgagta aataacgcat tcatgtcttc aggtttttat
     2341 atagcgtctg gagacacgga tatttatgca atgtaatcaa cgccagttgc aaaaaaatga
     2401 acccggatta cttatttaac cgcccatagc atcaacgctt acggaactct ttgttcactt
     2461 tcccacagca cttttaaaac attaaacctg actacggaaa atatcagcca tgataatgtt
     2521 tgaaatggct aatttgccat ggagtgaaaa aaattagatg aaattcagta ggttgaaata
     2581 atcactagca ggtaattatt tcaatgatag tgcgcaattg atctacaaca ctgcgtagcg
     2641 gagagagtat taatcggatc atagtcacat caagtgacta tgatccgggt gacaaccggg
     2701 gtaattattg ctgcttaacg aacaaactgg cgaagctgaa caggctggcg gcgctaaata
     2761 tcagttcaat tcccaccagt gtggaaacca gcgttacaga caccatcggc gttgcaccaa
     2821 ggaatatcca ggcaatgacg atatccagca caccaataac gagctgtagc cagctgcctt
     2881 tcattgaacg ctgacgatac caactcatca ggcgaataac ccctgcaaca cagaacaaac
     2941 cggcaataaa tgccgcaatg gcaaaaatac ccagctccgg tgcgcggatg aagaaatagc
     3001 cgatcaataa ataggcgact gcgacgagga aaccggataa taccggccag aaattatgac
     3061 tgcggttgct gaataacccg acaataagcg caatacccga gcagattaat aatgcaccca
     3121 ctactgtgct taaaatatcg ccagagacga acgggaaact gatacacagc aacccgacga
     3181 taaacagcag cacggcaata aactggattg ctctgcgatg ctttttcagc atctccagat
     3241 caaacttcaa aattgttgcc ttatctatat ataacataga accaccctat aaaattaaga
     3301 agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct aagaatacag tcgatttaat
     3361 aaaaatttcc taattgcagt atctgatgca tctgtaactc attgtattga aataaaaata
     3421 tctgattttg atattttcca tcaacatgac atatacagaa aaccaggtta taacctcagt
     3481 gtcgaaattg attcgtgacg gctctttcac tttatagttg aggatattac gatgaaaaaa
     3541 gtattaggcg ttattcttgg tggtctgctt cttctgccag ttgtgagcaa tgcagcggat
     3601 gcgcaaaaag cagctgataa caaaaaaccg gtcaactcat ggacctgtga agatttcctg
     3661 gctgtggacg aatccttcca gccaactgca gttggttttg ctgaagcgct gaacaacaaa
     3721 gataaaccag aagatgcggt tttagatgtt cagggtattg caaccgtaac cccagctatc
     3781 gttcaggctt gtactcagga taaacaagcc aactttaaag ataaagttaa aggcgaatgg
     3841 gacaaaatta agaaagatat gtaattccgg gaatgcgtta catcgtactt ccttgcatat
     3901 tgaacaggcc ggaatatctt ctttaaaagc agctattcct cctgttcata tataatctct
     3961 atattgaatg ggtcacaaaa tgaatatttc atctctccgt aaagcgttta tttttatggg
     4021 cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg ctggcagcca atgaatccgc
     4081 taaagatatg acctgccagg aatttattga tctgaatcca aaagcaatga ccccggttgc
     4141 atggtggatg ctgcatgaag aaacagtata taaaggtggc gataccgtta ctttaaatga
     4201 aaccgatctc actcaaattc ctaaagtgat cgaatactgt aagaaaaacc cgcagaaaaa
     4261 tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg aattaatgag gtacaagtaa
     4321 aaaggagtag caagttgagc catcttgctg ctcctttttg catttttata tgacagcaga
     4381 atttattata cgtcttatac tcgcggcaat tgcctgtggc gctattggca tggaaaggca
     4441 aatgcgcggc aaaggagcag ggttacgcac acatgtatta attggcatgg gaagcgccct
     4501 gtttatgatt gtttcgaaat atggttttgc tgacgtgctg tctttagatc acgtcggact
     4561 cgaccccagc cgtattgctg cttaggtggt gacgggcgtc gggtttatcg gtgcaggtaa
     4621 cattctcgtt cgtaaccaaa atattgtagg tctgacgacg gcagcggata tctgggtgac
     4681 cgccgcatag gtatggttat tggcagcggt atgtacgaac tgggtattta tggttcagtg
     4741 atgaccttgt tggtgctgga agtcttccat caattaacct tccgcctgat gaacaaaaat
     4801 taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga ctggtttaag
     4861 cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc
     4921 gccattgata tccagcttca tgccacaacg ttgatagaag acctgttgcg attattaaaa
     4981 ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg tagccagact
     5041 caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac cagctcattg
     5101 atccgcttaa tattgaggat catcatgata ttgtgtttat ggcagtagaa cgtttttagc
     5161 gatattttca gtttctccag aatatcttca gttgtcattt gattcgcaat gcagctcaga
     5221 acctgatgtt gcttgcgcga aagtgacaca ttcgcttcag gcacagagca gtaactgcca
     5281 ttgatgagtg cgacaaaatc aatgatagtg atagtccgcg gaacaaaggt caccggtact
     5341 ttccccagac agcgtttaat attaaagggg gagaaaacaa tgacatggac aggttttaat
     5401 cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt atcaataatg
     5461 acgaaggcat tctgatgcaa cattacgtcg atctcttctt cgttctgtat atgaacgaca
     5521 ttacctttac tcagaatgtt tttcatcgcg gtgaagaaca tcgtatccct ggtaattata
     5581 agaaacataa ccgactcctg gtgcggacat taactttata atcagtttgc caaaggtaat
     5641 taacaggaca gggcttataa taataatagt tagcttactg tatattaatt ttatgtaata
     5701 catgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg accagctcag
     5761 gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt tccggccaga
     5821 atgcaccata gccgtaatcc cacaggttat aggtggtatt aaccacttct ctcaaatgcc
     5881 acacctggat gccctgcata ttcacttcta ggaagttata cgggacttta ttgataaagc
     5941 ctggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga tagttcactg
     6001 gatcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct tcaatatcag
     6061 gcttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc gttttgccat
     6121 tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat aaccccggct
     6181 ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct ttgatatttt
     6241 gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt gcaccttttg
     6301 tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat atttctttat
     6361 agttttcatc tgattctgag gtgatatgcc tgatagtaac gtaaacctgt ctgatgttta
     6421 tatcccttct gtttagttca attccgttat tttggggcgt ggtatgagta tccatcgccc
     6481 aataatataa ccctttgttt taaaattaca taaaatctat ttttattcaa tgattataaa
     6541 gtgctaataa tcaatattat ggtattatat tctgataatg atttgataag cgaattcttt
     6601 tgccagatgg tatttctatt tccaccccat aatttatatc tttttcatta ggcagatctc
     6661 tctttaaata aaaggggtat gaaatctttt cgggttgaag tgtctcgtaa tgaaatgcta
     6721 tgaattcatt tttagacatt gttgcccatt tttgaatttt attgtataga gagggtgttg
     6781 taaactcttg aagttgttta taacatattt ttttgaactc ttgcttatat tttaatatgt
     6841 catcaaatga aagattcact acctttgcct ctaagaaatc aattattctc gaagcaattt
     6901 ccgctttcgc gcgtaaagag ttgtcattaa tagttaacaa tgtagaaaac aagccattga
     6961 tttgtttgtc aagatgctgt tcatatttat gtagtttggg gatgggattt tccttgtgtg
     7021 aaaaaatagt gttaaataaa tttgcgtcag tgaaatttat attaagtaga tcttcaaaat
     7081 aaaattttga taaggttagg actagcttgg acatatcttg ccctgtcatg ttgaaatcaa
     7141 taaagtcgca tccagcaagg gcttccatcc tattttcatt tttactgtgc tcaatataca
     7201 gcttcgccat ttttaagtac aagctttgaa tgtctcttat atcagttggt aagtcagctc
     7261 gttgcccagc aaaattttta ttttcggaag aaacggtttg gacactgaat ctttcgggta
     7321 ttccttcttc tacgagaatt ttagttatga ttggattgtt gggttctgga ttaacgacaa
     7381 caatgatttg acatacagaa tccttatcct tttcgtatct aatgttatga atctgattaa
     7441 gtattgattg tgtctgttgc gtaagtggtg tcgaacttat cgtttcaatg ttattaataa
     7501 gacttacaag ttgaaccaca acttttgagt agtctgcatt tgtatatgtg gtgttcgtgt
     7561 ggcatgcatt aatttgttgg agttgttgtt gcgctaaatc aaattgttca gggttgtatc
     7621 cttctccaga gaatgtggaa ttaatgggtt gttgttcttc ccaattatca caaggccact
     7681 tcggtgtgtc tggcgtttta acattatcgg tatttgttgt gcagttataa atatcgccgt
     7741 gatatgtatt tattatacct ggggtaaagt caattgacat aacattcctt attcatcatc
     7801 tgttttatgc taatgttttt aaattggata attagaacag gaatggattt tcttctacac
     7861 acaataaata taatgcgtta tatcttcctg atataattac ctttaaattc cctttcattt
     7921 ccacccggta atatttgtgt ataaaaaaag ggcagtgata gtatcgtaaa ggaaaacgtt
     7981 aacaactatc aaaaagcgct cattttaaaa agttattatg ttctccgata gtgtgtgaag
     8041 taatattttt gtgatgtttt agatgtgtaa taatgaacta ggttaaagtg tatttaaaaa
     8101 tgtgttgtat tagcgactga aatctgcctt ttaaaaagga ggagggtact gagacaaaga
     8161 gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt gtctgttgat
     8221 gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt gtgattttta
     8281 aggattatcc tgtctatgat tttctactta gaaggatatg gtactaacta tgcatcttat
     8341 agtttactgc tggcatctat ttaaaatagc aattgagtca aatagcccat caagatagcc
     8401 atacctaata taaccgtcag gaatgcagct atcatcggca ttctgaacat tgatttaagt
     8461 aatatcactt ccgtcaggct tgcgccggca ctgccgatga ttaatgccat taatgctccc
     8521 agacccattc cttttgtcat cagaacagat gccagaggga taaccgcctc agcccggata
     8581 tacagcggaa taccgacaac ggcgcttaat gggatggcga agggattatc tgctcctgca
     8641 tgagcggcaa tccacgctga gggaataaag ccataaataa aagatcctat taatactcct
     8701 aacaaaagat atggcagtac atctttaaac tgttgcaacg catctttggt ggctagtttt
     8761 atgggattaa tacttactac agctttggta ctgcaacagg taactacggg gttctcaatg
     8821 gctttagccg ctggactaca acaactcact tttatgggcg tatatgtcgt tcccggcgaa
     8881 gtttttgctg gagcacaaca atttgctgat gagcttttac tggcaatgat atgacgttca
     8941 aac
//
LOCUS       DADLSE010000151         8838 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLSE010000151 DADLSE010000000
VERSION     DADLSE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.152"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(158..1129)
                     /locus_tag="JN692_003432"
     CDS             complement(158..1129)
                     /locus_tag="JN692_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7265649.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN692_003433"
     CDS             complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN692_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7265650.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3036..4046
                     /locus_tag="JN692_003434"
     CDS             3036..4046
                     /locus_tag="JN692_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7265651.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4852..5181)
                     /locus_tag="JN692_003435"
                     /pseudo
     CDS             complement(4852..5181)
                     /locus_tag="JN692_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5299..6024
                     /locus_tag="JN692_003436"
     CDS             5299..6024
                     /locus_tag="JN692_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7265652.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6124..6534
                     /gene="uraH"
                     /locus_tag="JN692_003437"
     CDS             6124..6534
                     /gene="uraH"
                     /locus_tag="JN692_003437"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7265653.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6637..7596
                     /locus_tag="JN692_003438"
     CDS             6637..7596
                     /locus_tag="JN692_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAY7265654.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7742..8830
                     /locus_tag="JN692_003439"
     CDS             7742..8830
                     /locus_tag="JN692_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7265655.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2234 a         2336 c         2254 g         2014 t
ORIGIN      
        1 cgacgcaggt gggaccgtgg tcccagggag ataacctgag cggcgataca ggtgggaccg
       61 tggtcccaga gagaatattc aggccagtta tgttttctgg cctgtgagaa aggaaatgaa
      121 gtaaagggag atcaccgtag actaaagcgt gtccgcatca gactcctggt ttctcaagct
      181 ccttaagaat ggcttctatt ttctctatac actccgcagg aatgcgggac ctatccaggt
      241 taagcaccat tttatcgccc ttatacaatg ctgttgcccc tggagcaaac tgatgacgtg
      301 agctcagatt aactcttgat gcgggagatt gtttcagtac ggatgttaaa agactaatga
      361 cctcttcagc ttcaaagatt agtcccgctt tcttctgatc gtggagagtc tcagcctgct
      421 gcttcagtaa ttcttcttta tctgcaaaag ccttctgaag ggcttcacct gaccgggcag
      481 acagttctcc aggatgtgca aacagggcaa caacggattt aggcaatttg gccgtattga
      541 tacagcgggt aatgatctta cgagagatgt tttccgcatc ggcaagcgca gaaatatttc
      601 ctgcaaactc attctgcagc cggcttgtat aacgcaggcc acgttcataa gcactcgtcg
      661 gccgataatc gttacccagt cgggacaatg cagccatctg ctcatcgtcc agttcaccaa
      721 ccagaacgcg ataatcactt tccgtcagga ttgcggcttt acggcgacgg ctgccgtcgg
      781 caatttctat gacatcagaa acccggcggc cgaaagctgg agtctgctga ccggtcagta
      841 aaaatgaagg aatgagatca tccagagcat cttcggtaag tagttcctga tcgcgctcat
      901 tgcctgacca tacgcgtgaa gcgctctcaa cagagtctcc ccggagcacc tcaagggtaa
      961 atttcacttc ccgtccacat accggaagag agattgcatt accgcgggcc atagcaccca
     1021 cgcgcgcaat taatgaatcc accatcggcg cggccggcgc cggtgatgaa gtatcttcag
     1081 tcgattgact atgtgtggta tgtcttggaa tgacaggagc acgcttcatt atctaatctc
     1141 ccagcgtggt ttaatcagac gatcaaaaat ctcattgcaa acaggctccc agatagagag
     1201 ggcattacgc caggcaccgg ttgaagaacg ttgatcaata gcctgttcaa aaacggttct
     1261 catacggatc tgacctttac cgacttcatc cgtttcgcgc acaacatttt taaggaccat
     1321 gcttccccag gcatcccgaa tttgttcttc catccacggg gactgtgagc cattattatt
     1381 actgtatttg gtaagcagaa tacggacatc tggctcgaag cctttaagat ccacgttctt
     1441 aagcaggtca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcga acagctctgc
     1501 cggagtaggg acaatcagaa catcagcagc gcatacaaca tttatcgtac cgataccaag
     1561 gttgggtgca ctgtcgataa ctatcacgtc ataatcatga gcgacagttt caatggccag
     1621 acggagcatc aggtgcgggt cagcaggtaa tttgccttcg tcaaatttcc ccatcagctc
     1681 ggtttcaatg cggtgcagtg ccagacagga cgggatgata tcaaggccag gccagcaggt
     1741 gggctttata gcgtagctgg cgtcatcctt ttccccaaga taaaagggca ggagagtgtc
     1801 ctcagcatga atatgaagat cgggcaccca tccgtgatac atagaagcag ttccctgtgg
     1861 gtcattgcct tcaacaagca gaacacggag tcctttcaga gcaagatcct gagccaggtg
     1921 aacggatact gaggttttgt aaacaccacc tttgtgagct gcaacgccaa tcaccggcgg
     1981 aaacgcatct tcagcacggc gcagtcgtgt accaaataca tcccgcatgt gattgatttg
     2041 ttcgatggta taaccaacgc gttgctcaac tctcccacgg gtttccatat cagggtgcgg
     2101 cagccggcca gctttctcgg catccctgat agcctgagat gacacgccaa ctaaatctgc
     2161 cgcttcacca atcctccagc gccgggttat tttccgggct tccggactgt catcattaaa
     2221 ctgtgcaatg gcgattgctt tcgtcatttc atgaccagca cttatgcact ggttgagtgt
     2281 atccatgagt cccatactga acatccttta atcatcactt tgcattattt tattaaatct
     2341 tggaatttat tgcaaagcaa caataaaatc gcaaagcaca cgaaaaagtg caaagtgaac
     2401 cacattgggt aatgaaagaa cgcaggggat tacaaaagag tacataccgc agtcaatatt
     2461 aatcactgcc gctcgccgca gtaagcgatc tcacacagtg agaaagcggc gaggaagcgg
     2521 aaaatcacag aactcacaga ttgcacgtac gtcgggttgc taaagatgac tcgggatcag
     2581 ccagttataa tgaaattgac tgcggtatgt aatatattga ctatggtatg atgcttaccg
     2641 catctgtagg aaagacgact cagcataccg cagtcaatgt ttcattgttg tacctgtaga
     2701 tctcaatctt ccagagcgta ttcaggaaaa tcaggccagg ggtctggata tctggtgtgg
     2761 cgcttccggc cttgtatctg ggttattcat ggtctcgatc caataattga tcccaaggag
     2821 atcatagtga gatccaaaag agatccccgg atcgctccag catgcgctgt aactggcttt
     2881 aacgtctgtc tggatcgact gcggaatgta aaatgttgac tgttatatgc ataatctaat
     2941 actgtgatat gcaacggtat ttactgaggt atgtatatcc gttgactgcg atacgtatta
     3001 ctacatcctt ttgtggataa ccgttgagga tcaagatgac gagcgaaaat aatagcttac
     3061 ttctgaacct tcaggaagtt gataagacaa ccggtgaagt tgttaagctg gatgtcaaca
     3121 gcaccagtac cgtgcagcca gtagcgctca tgagactcgg tctcttcgtc ccaactctga
     3181 aatcaacagg gaagagcaag gcgaaccgga aaaacgttac agacgcgact gaggagcttg
     3241 tacagctggc cattgccaaa agcgaaggat acactgacgt taagatcacc ggttcgcgtc
     3301 ttgatatgga cacggatttt aaagtctggc tggggataat tcgctccatg tcggagtatg
     3361 gggtaaaaag tgacaccctg gaactgtcgt tcgtcgaatt cgttaagatg tgcggatttg
     3421 actcccgtcg ttcaaacaaa aaaatgcgcg atcgcatcag taattccctg tttaaactcg
     3481 cctcggttac gctgaagttc cagagcgaga ccaaaggatg gaccacccat ctggtgcagt
     3541 cagcctatta tgacatcaac gaggatatcg ttgagatcaa ggctgaacct aagctgtttg
     3601 agctttacca catggacaga agggtactgc tgcggctgaa agccattgac gccctgcagc
     3661 gcaaggaatc agcccaggcg ctttatacct acatcgaaag tcttccccag aatcctgcac
     3721 ctatttccat gaaacggatg cgtgaccggc tgaatctcac ctcaaatgtc tatacacaga
     3781 accacacggt gcgtaaggcg atggagcagc tgcgtgatat tggttatctg gattacaccg
     3841 agtttaaacg tggccgagcc acctatttca gcgtgcatta tcgcaacccc aaactcatca
     3901 gtagtcctgt gaaggttccg cgcaaagagg aagaagagaa ggcgccagag caaaattatg
     3961 acgaagtcat taaggcgctg aaggctgctg gcatcgatcc tcttaaactg gctgaagccc
     4021 tctcagcgat gaagccagaa aattgactgc gatatgcata tcatgactgc cgtatgccgc
     4081 taaggacatt ccattgactg tgatatgcat cacgcatacc gcagtcaata tttattctct
     4141 atcatgtctg cccggatcga acaatattga ctgtcatatg tgatttcggg tcatgatacg
     4201 cagatattga ctacggaatg cgggtttaat gggggtaatc cctggtctgt tcaccattcc
     4261 atgggggata acatgccaca gtcaattttt cagtaacgct ctatatccct tacgaaaatc
     4321 tcaatcgggt gctgagacgg tcatgttacc tgacggtatc cgttcccctg tgtactgcag
     4381 gtttagcccg gcaggatgag actgcagctg tctgtataac ccggcgccag cttcacactg
     4441 ttaaatgcga tcaggaaacg ggagccgaat ggcagaaagg tgttggctag ggaaggtgcg
     4501 aataagcagg ttatttcttc ccaagctgac tcgctgatta aaatttcgcg gatctgggcc
     4561 gatttttttc ccgcaaacac atcgaatcag cctatttagg ggtcagtttg gagatcggaa
     4621 atttttgtac gttaagcaca ttatttaacc aattcggata cgtaagtgat tgatttatcg
     4681 tcattggcta tcgcttttag gttgtcgctc ttgctgtatc gcatttaggt tatccgatta
     4741 agacaaaatc ccctgaaaac gctgtatagc gcgggagtac accctattgc tacaggtaag
     4801 cctgttcaaa aaacaggctt accgtacaat aattctctat atccaaactg accccaagaa
     4861 ggacaccttc agcgcggccg tggtcggttc gccggtaaag taggctttac cctcgctctc
     4921 attccacttg ttcttcacag ggtcgtagcc gcggttaagc acgctgatgc cgccgtcgct
     4981 gcgcttgccg taggtggcgg taacctgctc taggccgcgt tcaaagcggt tttccaggcg
     5041 agcgacttcg taccatttgc caagatagcg gctggcgtca aaaccggaaa tgggctgcac
     5101 gcccttcggt ggggtagggg atttacaggc taccagcgtc agggcaatgg caacaccggt
     5161 cacaacaggc catagcttca tgagaattcc tttcatttgc acggttggaa attaagtgta
     5221 gtgcaccgcg gcggcgcttc atcccatccg cgaaaattcg tagtatattg agagtattcc
     5281 tcaactctgg acacggacat ggcttactcc atcggcgaat tcgccagact cagcgggatc
     5341 accgccacca cgctgcgggc ctggcagcgg cgctacggct tactcaagcc tcagcgcacg
     5401 gagggcggcc accgccagta cagcgatgag gacgtccagc aggcgctaaa aatcctcgac
     5461 tgggtaaaaa aaggcgtccc gattggccag gtcaaaccgc tgctggagcg ccctacgtcg
     5521 cgcctgacca acaactggct cacgctgcag cagagcatgt tacagcgcct gcaggacggc
     5581 aaaatcgagt ccctgcgtca gatgatttac gacgcaggac gcgagtaccc aagaccagaa
     5641 ctggtcgcga acgtgctgcg tccgctgcgc agtcaggtgt cggcaaacgt cgccgccgcc
     5701 atgaccctgc gggagatcct cgacggcatc atcatcgcct acacctcgtt ttgcttggaa
     5761 ggcgataaaa aagcgccggg cgacaacatc ctgctcagcg gctggaacct gaatgacccg
     5821 tgcgaagtct ggctcgaagc cttaacccgc accgggcaag gccaccgtat cgatatttta
     5881 cccacgccgc ccgccacgct ggccccggag attttcccgg accgccactg gcttctggtc
     5941 acgcgcggta aactcaccgc cgcgcgcaaa aagcagatcg agcagtggca gcagcaggta
     6001 tcgcttgaag tgattatcct ctaatcattt tctcctgcgg gaatcttcac gaaaagttga
     6061 atattttccc gcgccacaaa tgctaacgtt ttcctgtgac cgacaacccc acaggaaaac
     6121 acaatgaaaa aaactgcctc tctgctgatg ctgaccaccc tggcctttgc ccctgccgcc
     6181 tttagcgccc ctgccggcac gctgagcgta cacattcttg accagcaaac cgggatgccg
     6241 ccatcggacg ttaccgtcac gctggaaaaa cagcagcagg acaaatggac cccaattgcc
     6301 agcggcaaaa ctgaccatga cggccgcatc aaatcgcttt atccggagga tcaggatatg
     6361 cagcccggcg tctataaagt ggcctttaaa accgcagatt acttccacgg gaagaagctg
     6421 gactccttct tcccggagat cccggtgctg ttcaccgtaa cccggaccaa cgaaaagctg
     6481 cacatccctc tcctgctgag ccaatacggt tattcaactt acaagggcag ttaagaaacg
     6541 ttgattttaa aggcaaataa aaaataattc cgctaatact tggacaaatt agttatgttg
     6601 tgtaagtttt aaagagaaga acgggaggac cacaccatga caacaaaacc tgtgctcggt
     6661 attagcggat gtttgaccgg gtctgccgtt cgcttcgacg gcggacacaa gcgtatgggc
     6721 tttgtcatgg atgaactggc ccagtgggtg agtttcaggc ccgtctgccc ggaaatggcg
     6781 attggcctgc cgacgccacg cccggccata cgcctgacgc tgacggacag cggcgaaacg
     6841 cagctccgct tcagcaagcc gccccatgat gatataacgc agaaaatggc cgactttacg
     6901 gcggactatc tgccaaaaat cggcgatctg tcgggattta tcgtctgcgc gaaatcgcca
     6961 agctgcggca tggaacgcgt gcggctatat gatgaaaacg gcaaccgggg ccgcaaggag
     7021 ggtgtcgggc tgttcaccgc cgccctgctt gaaacctacc cgtggctgcc cgtggaggaa
     7081 gacggtcggc tgcacgaccc ggtgctgcgg gaaaacttta tcgagcgggt atttgccctg
     7141 cacgagctga acacgctgcg cgctaaaggc ctgacgcgcc gcgcgctgct ggatttccac
     7201 agccgctata aattgcagct tctggcgcac catcaggcgg gctaccgcga gattggcccg
     7261 ttcgttgcgt cgctgcatga atgggaagat ctggacgcgt tcttcgtggc gtaccgcgaa
     7321 aagctgatga ccatcctgaa aaagcccgcc tcgcggaaaa accataccaa cgtgctaatg
     7381 catatccagg gctatttccg taaccagctg aacagccgcc agcgcgtcga gctgcgcgat
     7441 gtgatcctgc actatcgcga cgggctgcta cccatcctcg cgccgttgac gctactgaaa
     7501 cactacatgg ccgaataccc tgaccggtat ctgatgacgc aaaactactt tgatccctat
     7561 cctgatgatt tgggtctgcg tctggcagta acctgacgat aaaaatgcga aggcccgttt
     7621 gacccacaga ggcccaaagc agtaccccgt ttagacgaca ccctcactgt cgtctctttt
     7681 ttgcattcca cacaaaagac tgtaattatg tacaggattg taacgcaaaa agggactgcc
     7741 tgtgatcacc accctgcaca ttcaaaacta ccgctcgatt cgcgaaatgt cgctggagct
     7801 ggaacagctc aacatcgtct ttggcccaaa cggcaccgga aaatccaata tctacaaggc
     7861 gatccacctg atgcacagcg ccgcacaggg gcagttttcg caggcactgg cgaacgaggg
     7921 tgggatttta aaagtgttct gggcaggcaa aacccgcagc gaccagctgc ggcggatgaa
     7981 tttggccgtt gaaaccgaaa cctacgaata cgagctgcag gtaggctttg tcgaaaaact
     8041 gccttacccc tcacagtttc aactcgatcc ggtgatcaaa gaagagtcca tctggctgag
     8101 tggtcaatac cgccgcccgt cgtcacagct gatgaagcgt aaaaatcagg ccgtatttct
     8161 gaacaacgtg catcacgaga aagtcaccca cagcggcacg ctgtatgaga acgaatcggt
     8221 gttcggccag cttggcgagc cgcatcttta cccggaagtg tcgcagatgc gcgagtcgct
     8281 gcgtaactgg cgcttttatc aggagttttc tgtatcaatc ggctcggcca tgcgcgcgcc
     8341 tcaggtcggt ttccgctccc cagtgctggc cagcgacggc gcaaacctgg cggcggcgtt
     8401 tcagaccatc gtggaaattg gcgacgagct gctgctgatg cgcatcctcg accaggcctt
     8461 ccccggctgc gtgttttaca gcgacaacac cggcggacgc ttccggatga tgatgcagcg
     8521 tgaagggcta agcagaccgc tggagccggc ggaattctcc gacggcactc tgcgttttct
     8581 gtgcctggcg gtggcgctat taagcccgcg tccaccggcc tttatcgccc tcaacgaacc
     8641 ggaaaacagc ctgcatccgc agatgctgcc cgcgctggcg agcttaatcg ccgaggccag
     8701 ccgctactcg caaatttggc tgaccagcca ctcgccggaa ctggcgaatt taattgagaa
     8761 acagagatcg ttttcgctgt atcagctgtc gatggtggaa ggagaaacga aggtggagag
     8821 gctggggtag tctcccag
//
LOCUS       DADLSE010000152         8771 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLSE010000152 DADLSE010000000
VERSION     DADLSE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.153"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(524..892)
                     /locus_tag="JN692_003440"
                     /pseudo
     CDS             complement(524..892)
                     /locus_tag="JN692_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1418..2392
                     /gene="hemB"
                     /locus_tag="JN692_003441"
     CDS             1418..2392
                     /gene="hemB"
                     /locus_tag="JN692_003441"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7265656.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN692_003442"
     CDS             complement(2498..3349)
                     /gene="tauD"
                     /locus_tag="JN692_003442"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HAY7265657.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN692_003443"
     CDS             complement(3346..4173)
                     /gene="tauC"
                     /locus_tag="JN692_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7265658.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN692_003444"
     CDS             complement(4170..4937)
                     /gene="tauB"
                     /locus_tag="JN692_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7265659.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLLLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN692_003445"
     CDS             complement(4950..5876)
                     /gene="tauA"
                     /locus_tag="JN692_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7265660.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQVDNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSGID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(6144..6659)
                     /locus_tag="JN692_003446"
     CDS             complement(6144..6659)
                     /locus_tag="JN692_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265661.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARAWGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6853..7104
                     /locus_tag="JN692_003447"
     CDS             6853..7104
                     /locus_tag="JN692_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7265662.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            7106..8771
                     /locus_tag="JN692_003448"
                     /pseudo
     CDS             7106..8771
                     /locus_tag="JN692_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2206 a         2279 c         2173 g         2113 t
ORIGIN      
        1 tctgcatagc attatcagca ttcggatgtg caactacagc taatgcaaca tcatcccagc
       61 ttgcaatagt gctatcagca acaccatctc cgtcagtatc tacggtgaca aagttagatt
      121 taccactata atcgctggca ttacctgtat taccgaacca accagtagta ccttcatcag
      181 tccatgaacc agaggttacg gtagaatctt taacagtgat ggtgttatta aacacttcgc
      241 tgctgtttac gccagtgctg acagtgttgt cgtcaatatc ttcccactca taaccctgag
      301 tcaacgtaat acccgcaacg tgagagttat tagtgatgga cagatcaact tcctgatcca
      361 gagtaatagc cgtaccaatg ttgtaaacgt ctacaacatg agtatcagcg gcattattgt
      421 aagtgccgtt atatgtgtag tgttcgtagt tgtcgtcgat cgttgaatta tcaacagtca
      481 gcgccagacg gtcatggtag taatcatccg cacgatcagc acaatcagtt gtcatgcact
      541 cggaataaat cataccgtga atagtgctgt ttttgattgt cagactattg gcgttggtat
      601 tggtagaaat accgtcgtcc agatagtagg tagaaatcac gccgttaacg gtagcgttat
      661 taatgaccgg gtagatatcg ccgttgtaat agctatcagc ggcatagttg ttccagccta
      721 cataaccatc atagtaaacg tcatcagcat aggtggcgtc attgtagtga tggaaagtat
      781 tgtaagtttg accagaaata tcgttggtcg ctgcattagc ctgagaggtg atagccagag
      841 tgcaagccaa tgctaattgt gatactacaa gtttcttttt ccaggagtgc atttgtcatc
      901 cctcctcagg gacgtaataa gttgatccat caattatcaa tgcatagaaa acatattatg
      961 tttcgcgcta acgattatgc tgcgttacag acgaatggtt caattattct ttcccctaag
     1021 tccgaatgtg gacatatata atttatggag aaaaacacgc atcaaattga taaaaggacg
     1081 atatttcagc atgttgcaga taatgttatt tttgatacat acgcgttata aatatcaaaa
     1141 acacaaacca gataaatatc ttatcaatat acaacaaagt ttgaatcatt caatttatag
     1201 cagcacaacc aaacgccaaa aaaaataata accaagagct ataaataaag attaaaaata
     1261 ttttcagtga atatgaaatt attgctattt ctattacaaa gatgtcctgg tctctctttc
     1321 attctcttct tgtcaacatc gcgacaactt tcgtaaaaca ctcctgcccc gcttcaggta
     1381 tactgtgcac cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc
     1441 tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct
     1501 taacgacctg gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga
     1561 agccatgcca ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat
     1621 cgccaacgcc ggtatccgtt ccgtaatgac ttttggcatc tctcaccata ccgatgaaac
     1681 cggcagcgac gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca
     1741 gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg
     1801 tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg
     1861 caagcaagcc gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat
     1921 ggacggccag gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc
     1981 gattatgtcg tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc
     2041 cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgcga
     2101 ggcgattcgt gagtcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc
     2161 tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg
     2221 cgcgtatcag gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat
     2281 agatgaagag aaagtcgtgc tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct
     2341 gattttcagc tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc
     2401 ctctcagaaa gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc
     2461 tctccccaag ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt
     2521 tatccccaag gatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg
     2581 catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac
     2641 gcacctgaaa ctccggttta gtgatatggg cgaataaaaa gcctaacaag gcttcgctct
     2701 ctttctcgct gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt
     2761 taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg
     2821 cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaatgatt
     2881 tacggaaatc atgctccgca cgcagccagc tcagcagctg gcggaaagga acagagagcg
     2941 cctcataggc cgcaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt
     3001 tagctgccag aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt
     3061 tgtcgttatc cggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt
     3121 cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt
     3181 gctgctgcgg cgtaatagct tgatcgcgca gaaataccac ctgatggcgc agcaccgcat
     3241 ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt
     3301 gtgcgccgat atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc
     3361 atgccagggc gtcagacggc gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat
     3421 aatcgcaatc accgcgatcc ccgccagtac cacgtcagtc gcgagaaatt cacccgctga
     3481 ctgaaccata aatcctaaac cgcgtgtcgc ggcaatcagc tccgccgcta ccagcgtaga
     3541 ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa
     3601 aatgacaaac cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg
     3661 aacctgctgc gcgcttttca cccctgccag cgccgacatc gccaccggcg caaaaatcgc
     3721 taaatagatc agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg
     3781 caaataagcc agcggcggca ccggacgata aagttcgatt accggatcca gaatgccgcg
     3841 caccgtcggg cttagcccca tggcgatccc gaccggaata ccgatcacca ccgccgcgag
     3901 tagcgccagc acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat
     3961 aaatccctgc ggtccggcaa tggtgagcag ttttgccagt acttgttgcg gcggcggcaa
     4021 aaacagcggg ctaatcagtt gcagcgccgc caccgtccac catacagtga gtaaaaccgc
     4081 taacgtgcca atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgccg
     4141 cgaatgcagt ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg
     4201 cggcttaaaa catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc
     4261 gactctcccg caacaaagcg gcgagcaaag ttgagcagca gccgctccag cacacggcca
     4321 gggccggatg aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg
     4381 gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt tcagcagcag agtttgcatc
     4441 tggtcgcggg tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga
     4501 ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt gaccatcgga aagctgccag
     4561 atatagcgtt tttctgcgcc ttccagcccc acttttttca gcatctggtg cgcgatttcc
     4621 agtcgctgca ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca
     4681 ttgcgccacg gtagcagccc ttcattctga aaaactacgc cacgctctgc tcccggtccc
     4741 tcaatacgct tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accggcaatc
     4801 agattcagca gggtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg
     4861 ctttccagcg tcaggttgat atcttccagt gccggtttgc cgccataatc ggcatagaga
     4921 tgagagattt gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg
     4981 ctgtaatcat tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg
     5041 atgattgctt tgttcaccgg tccggtcagt tctgccgttt gttgctgcgg cgtcagatag
     5101 gtatttccct tcaccagccc cggaacgtca ccttcaggca cgccgcttaa acgcgccagt
     5161 ttgctgatgt tttccggctg tttcagccac gcgtcagggt tagcaatgta cggttgctgt
     5221 gcatcgatgc cgcttttggc gaacgctttc acgacctcag gatgtttctc ggcaaaatct
     5281 ttgcgcacca cccagacgtc cagcgttggc gcaccccact gcccgacctg ttcagaatcg
     5341 gtcagcacct tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca
     5401 tcaatatcac cccgctgcca tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc
     5461 actagccctg gtttaatccc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg
     5521 gagataaacg gtacggcaat gcgtttacca atcaaatctt cgggtttgct gatagttttc
     5581 tttaccacca gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc
     5641 acctgttggc tggctgcaac cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg
     5701 ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacgctc
     5761 gccccgctct ctttagcaaa ggtgttgtcg acctgagcca ctttcgccgg ttcggctgag
     5821 gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg
     5881 gcaagaagtg tgttatatga ctaggtaatt tattcaagat aaccgggaat gggtaaaaaa
     5941 agacgggatt tgatgggatt caataagtta tgcgccgatt gcattcaaga taaaacgagt
     6001 taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc cgtgtatttt
     6061 aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat tttaaaggcg
     6121 accctaaagg caggtcaatg aagttaaccc gccttcgcgt ttacactctc tgagagaata
     6181 ctagcttcaa tctcttccaa aatctctctg acttgctcgt cgggcatatc aacgagcatc
     6241 aacgctgcca ggcgtttacg gccctctagt tgcagtagct cttgagtttc cttatctaac
     6301 aagcgtagca cagtctcaac acctttcctt gcgagcatgt ttgtaagggc ttctcgttcc
     6361 tcactggtaa gaaacgacaa catttctgtt agctgatccc cttggcttaa atcactttgt
     6421 gttttttgtg tttttggtgg attcccgtta ccggttttta gccattcaag actaacacct
     6481 tcactttctg caatgcgtac caatatgctt tctcgaggtt ctggtgtatt tcccttttta
     6541 aaatagcttt gtaacgtatt gatatttatg ccccaggctc tggcggcagc acttcttgat
     6601 ggataccgcc taataagata agccatgcgc tccacgatag gttcttttgc ctgctccata
     6661 aaagtacctc taggtatttt tctgtataaa ggttctttcc catctatcta attgaatgga
     6721 aaggtaattt taaaaatcca aataaaatac aaaaaaaata ccttgtattg gtgctttttg
     6781 tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt aattttgtag
     6841 ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc gctgatgatt
     6901 cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact ccatgccggt
     6961 tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata cgaacgtgtt
     7021 atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta tcgtaacgat
     7081 ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg acctgctcgg
     7141 tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc gtttttctcg
     7201 tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt accatatcga
     7261 ttgcctgcct aaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac aactggtggc
     7321 tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta accctgatgc
     7381 tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac gcctgaatgc
     7441 cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag tggaagtgct
     7501 gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc tggttgaaaa
     7561 agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg ctaatgcaaa
     7621 acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac tgaaatacaa
     7681 ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac gcgacgaaat
     7741 aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc aggctaacgg
     7801 cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc gctatgcgga
     7861 cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg ccatgaaaaa
     7921 attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc gccagcttga
     7981 gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga ttggtgacgg
     8041 ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt caccggaagt
     8101 cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg atcttgcgga
     8161 gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg gaaaaccctt
     8221 cctgtattac tcggataatg gttccggtga aacggcagat atgctggata agagatcgtc
     8281 gggattctgc cccgtctggg tattaaccac cccaccggta ttgccggaaa tccgcaggga
     8341 cgcggcatca ttgaacgtct gaaccggacg ctgccgatgc gtatagccag acggtatcgc
     8401 acctattttg gcaagggggc agaccgggaa acactgcgtg tactcaatcg cgatctgcgt
     8461 tcagcattta atgccctgca acaggacaaa ccgctgaatg cccgccagaa atctgcaatg
     8521 cgcgagctgc cgtcctgatc agaactgatt gaggctattc gtgaaggagt ggaatggtac
     8581 aacaaccgcc cacattctga actgccgatg aaaccaaacg ggaagcatta cagcccggct
     8641 gatttcagaa aaaaacgcct ggcggaagaa aacaccgaaa ttgaatggct gagtgatctc
     8701 gaactgcgcg acatgttctg cccgatggtg gagcgtccgg taagacgctg tgaaatcaaa
     8761 tggcttggga a
//
LOCUS       DADLSE010000153         8576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLSE010000153 DADLSE010000000
VERSION     DADLSE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.154"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN692_003449"
     CDS             complement(204..950)
                     /gene="cutC"
                     /locus_tag="JN692_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7265663.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(964..1530)
                     /locus_tag="JN692_003450"
     CDS             complement(964..1530)
                     /locus_tag="JN692_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7265664.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1746..3479
                     /gene="argS"
                     /locus_tag="JN692_003451"
     CDS             1746..3479
                     /gene="argS"
                     /locus_tag="JN692_003451"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7265665.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAAYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3656..4144
                     /locus_tag="JN692_003452"
     CDS             3656..4144
                     /locus_tag="JN692_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HAY7265666.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4262..4654)
                     /locus_tag="JN692_003453"
     CDS             complement(4262..4654)
                     /locus_tag="JN692_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HAY7265667.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4654..6036)
                     /locus_tag="JN692_003454"
     CDS             complement(4654..6036)
                     /locus_tag="JN692_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HAY7265668.1"
                     /translation="MSMGHAAESYTLLTIGDGLVAQIPALVISTAAGGIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVNFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6033..6653)
                     /locus_tag="JN692_003455"
     CDS             complement(6033..6653)
                     /locus_tag="JN692_003455"
                     /inference="COORDINATES: protein motif:HMM:NF012973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265669.1"
                     /translation="MILLILSMMVLPLPAFILDLLFTFNIALSIMVLLVAMFTQRTLE
                     FAAFPTILLFTTLLRLALNVASTRIILMEGHTGAAAAGKVVEAFGHFLVGGNFAIGIV
                     VFVILVIINFMVITKGAGRIAEVGARFVLDGMPGKQMAIDADLNAGLIGEDEAKKRRS
                     EVTQEADFYGSMDGASKFVRGDAIAGILIMVINVVGGLLVGVLQLA"
     gene            complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN692_003456"
     CDS             complement(6678..7871)
                     /gene="flhB"
                     /locus_tag="JN692_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HAY7265670.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLVLLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRKPWILLTRNRPMSNLAAMLRLPA
                     NLKST"
     gene            8107..8490
                     /locus_tag="JN692_003457"
                     /pseudo
     CDS             8107..8490
                     /locus_tag="JN692_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
BASE COUNT         2205 a         2295 c         2085 g         1991 t
ORIGIN      
        1 gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt agcacaacgc
       61 tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta tactgctcag
      121 ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg
      181 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      241 tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg ttcatctgat
      301 gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag
      361 ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc acggactcct
      421 gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
      481 ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgca
      541 agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg aaaagtcact
      601 gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac attcccgtca
      661 acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt gcgcacatcc
      721 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt
      781 atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc caacgacggc
      841 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
      901 gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct
      961 taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat gaaacttaat
     1021 agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat gctcaccttt
     1081 tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag aaagcaatgc
     1141 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1201 accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg aaaactgcca
     1261 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattgatcat
     1321 attttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct cagcggtgac
     1381 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     1441 acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg
     1501 cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag taaaagcgtt
     1561 aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     1621 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     1681 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     1741 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     1801 gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag ttcggcgact
     1861 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     1921 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     1981 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2041 cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact
     2101 actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg
     2161 gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2221 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2281 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2341 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     2401 gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga
     2461 accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     2521 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     2581 tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac
     2641 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     2701 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     2761 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca gcctatgtcc
     2821 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa gacggcaaac
     2881 cgttcaaaac ccgcgcgggt ggtacagtga aactggcaga cctgctggat gaagccctgg
     2941 agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac gagctggaaa
     3001 aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca
     3061 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3121 acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa attaacgaag
     3181 agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa ctggcagctc
     3241 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa
     3301 tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga
     3361 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacag
     3421 cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag cgtatgtaat
     3481 cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt ttattgccat
     3541 ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta agaggccaga
     3601 agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata ccgcagtgtt
     3661 taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag aagatacggt
     3721 tatagtaaac gaccatgaca tttcagccat caaagattgt tggcaaaaaa attcagatga
     3781 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     3841 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     3901 tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     3961 ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc aggatttaag
     4021 tgacccttat tctgaatcag aatggctatc atgtatgatc atacagacca atacgcgaac
     4081 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4141 ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca
     4201 aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa ttttttgtct
     4261 ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgga attaaccgcc
     4321 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
     4381 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
     4441 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
     4501 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
     4561 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
     4621 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
     4681 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
     4741 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
     4801 cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4861 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
     4921 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4981 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
     5041 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
     5101 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
     5161 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
     5221 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
     5281 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     5341 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     5401 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     5461 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     5521 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     5581 caacaactca ccatcctgct ggaaattgac catcgggatc agtcgataac ccacttccat
     5641 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     5701 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     5761 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     5821 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     5881 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     5941 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     6001 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     6061 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     6121 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     6181 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     6241 tgcttacccg gcataccatc gagaacaaag cgcgccccca cttctgcgat acgcccggca
     6301 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     6361 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     6421 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     6481 aacaacgtag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     6541 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     6601 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     6661 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcagcc
     6721 agattactca tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg
     6781 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     6841 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     6901 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     6961 ccaatttcac gaatacgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     7021 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     7081 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     7141 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     7201 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     7261 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     7321 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     7381 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     7441 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     7501 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     7561 agcgccacca gcaccacgcc gctaatcagc ggtagcagca ccagcatggc ttctctgatc
     7621 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     7681 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     7741 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     7801 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     7861 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     7921 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     7981 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     8041 cctatagaat cgccctccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     8101 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     8161 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     8221 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     8281 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     8341 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     8401 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     8461 cacgatctcg gtaatccacc atttgggcat ctgaagtggt caacaaaaac tggccaccga
     8521 gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac cgttat
//
LOCUS       DADLSE010000154         8555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLSE010000154 DADLSE010000000
VERSION     DADLSE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.155"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..649)
                     /gene="yihS"
                     /locus_tag="JN692_003458"
     CDS             complement(<1..649)
                     /gene="yihS"
                     /locus_tag="JN692_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7265671.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRV"
     gene            complement(666..1544)
                     /gene="yihT"
                     /locus_tag="JN692_003459"
     CDS             complement(666..1544)
                     /gene="yihT"
                     /locus_tag="JN692_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HAY7265672.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(1568..2464)
                     /gene="yihU"
                     /locus_tag="JN692_003460"
     CDS             complement(1568..2464)
                     /gene="yihU"
                     /locus_tag="JN692_003460"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7265673.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVARTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            2632..3528
                     /gene="yihV"
                     /locus_tag="JN692_003461"
     CDS             2632..3528
                     /gene="yihV"
                     /locus_tag="JN692_003461"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7265674.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITSQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3562..4347
                     /locus_tag="JN692_003462"
     CDS             3562..4347
                     /locus_tag="JN692_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7265675.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            4446..5045
                     /gene="yihX"
                     /locus_tag="JN692_003463"
     CDS             4446..5045
                     /gene="yihX"
                     /locus_tag="JN692_003463"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7265676.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            5039..5911
                     /locus_tag="JN692_003464"
     CDS             5039..5911
                     /locus_tag="JN692_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7265677.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            5908..6345
                     /gene="dtd"
                     /locus_tag="JN692_003465"
     CDS             5908..6345
                     /gene="dtd"
                     /locus_tag="JN692_003465"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7265678.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            6390..7331
                     /gene="fabY"
                     /locus_tag="JN692_003466"
                     /gene_synonym="yiiD"
     CDS             6390..7331
                     /gene="fabY"
                     /locus_tag="JN692_003466"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7265679.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            7747..8555
                     /locus_tag="JN692_003467"
                     /pseudo
     CDS             7747..8555
                     /locus_tag="JN692_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2090 a         2071 c         2240 g         2154 t
ORIGIN      
        1 taacgcgata atgattattt ctggcgacgt cgtggataat cacggaagcc acgcgaatcg
       61 cgcgatccag ccattttttg tcgtgagtga cgtcataaac aatcaagaaa gcttccaccg
      121 cgtgcatatt ggcattgccg ccgcggtact cttcggtttt gctgaaggct tcgtcccagg
      181 attccaggca catctgctct tcttcgctcc agaaatattt ctcgataatc tcaatggtgt
      241 aatcgagcag cttgcgcgct tccgggtgac ccgttgtgac ggcgctggcg gcacccagca
      301 gagcaaagaa gtgttgatag ccctgtttgg aggcatccac cacgccctcg tcattcacgc
      361 aggcatacca gccgccatat tttttatcac gcagtgcgcc gttcatggct ttaataccgt
      421 gatcaaccaa cgcgtaagcg ccaggtcgac ccatcgccgc agcaacggaa taaacgtgca
      481 acatacgagc tgtgatccac agatgggtgc ccatctcttc tttgatttgc cctttattgc
      541 ctaaccagcc aaaaccagtc ggcactacgg aatttttacc aaaatcaaag atgcggtcgg
      601 tttcctgttc cagccaacgg ttgtggctta gggtgttaaa ccatttcatt ccttttatcc
      661 tcatcttagc ggcgtttagc catcatttcg tcgacgatat cgccaagttg ttgtaatttc
      721 ggtgcgcaaa cgtcacggag catcagctcg ttgtctggta aaccgacgac cgatgcccag
      781 actgcacggc ctgccaggaa tcccgatgcg cccgccgtca ttgccacgcg tacggcacgc
      841 gggaacagtt tttcgtcgac accggaagag aggatcaccc atggcatatt gatatggtca
      901 ttcagacgtt gtgaagcaca gagaagctct tgttgcggac ctttgccata aagcggcatt
      961 tcaactttgt agaggtcagc gccactgtcg cccagctctt tggcggcatc gatgatcgct
     1021 tgttcgcgat cgaatttatc gccacgacgc ggtgggcgga cgactggctc aatgatgctt
     1081 accagaccgt gtgagtggca cagttcgttg aactctttca ccatatccag acgttgctgc
     1141 gcatcttcat cactacgcca cagcaccagc agttttaagg ctttgccacc gtcttgtttg
     1201 atctgtagcg gattgatttt gcggtcaata accacgctat cgaccggaat accgttgcca
     1261 ggaatgaact catcggcggc gacaatcatg gcgcaacttt tggcaatcgc gttttgctca
     1321 accacctggc ggtagcagaa ttgttgatct accagaatcg ccgaggcata aggcgagagg
     1381 gcctttgcag cgttaacttt gaaatcagtt aaaacgctat cggctaccgg agcgggtgcc
     1441 ccagccgcgg caaacatcat gcgcatggct tcgcgctgat cgaccgccag catggcaaaa
     1501 ccgcccgatg cgcgcgtaat gtcgttgatg gtgtacttat tcattcattt attcctttat
     1561 ccagtcgtta catttttact ttggcagtca tcccggcact gacacggacc tgttccagaa
     1621 tggcggacca gtcctggcga ccgcgacccg ctgcgcgcgc ctggctataa acctcccgtg
     1681 aggcggcccc cagcggcatt ggcacatgca gctggttggc gacatcaagg gcgatgccaa
     1741 gatccttatg ggcaagatcg atcatgaagg cgggagaaag atctccgctg aggactttgt
     1801 ttggccagga agtggtgaag tgacctttac cggcggcggt accgctcatc actttgacgg
     1861 caacatcgaa gggaagattc agggcttcgc acaaaacggc ggcttctgcc gaaagcgcat
     1921 tgagcgcgat gctcatgtag ttgttgatga gcttaacgcg gatccccatg cccggaccgc
     1981 cagcgttgat caactcactg cccatcgcca tcaggatcgg tgtggcacgt tcaacttgtt
     2041 cagcggtgcc gccagccagc agtaacagag taccagtaat ggcatttgca gaagtacggg
     2101 ctaccggaac atccatcatg ctgaagcctt tggcttgcat atcggcaatc aatttatcgg
     2161 tttgcagcgg atggatggtg gacatatcaa tgaccagcgc atcggtagat aagccttcgc
     2221 aaacgccgtt ttcaccgaac aacacgttgc gcaccagatc gccattcggc aacatggtaa
     2281 tgataaattc ggcatcttta gcggcctgcg ccgggttggc ggcgggagtc gcacctttgt
     2341 ctaccagatg ccgcacagct tcggcgttca catcaaagac gcgaagttga tgcccttgct
     2401 gcaataaatt gctcgccatt ggcgaaccca tttgtcctaa accgataaat gcgattgctg
     2461 ccataaccct ctcctgaata cagtgatgtc actttttgtc atttatgaca tgctttgctt
     2521 gtctgttttt gatcgcattt gtaatttatc gtcaaaaaat tgacagccgt cactttttaa
     2581 acaattggtg aaattaaaat gaacgcatcc caaaatgttt aaggaatgac catgattcgt
     2641 gttgcttgtg taggtataac cgtgatggat cgcatctatt acgtggaagg gttaccgacg
     2701 gagagcggta aatacgtggc gagaaattat acggaagttg gtggcgggcc agcggcgacg
     2761 gcagcggttg cggcggcaag gctgggggcg caggtcgatt ttattggtcg cgtaggtgat
     2821 gacgacgcgg gcaacagcct gctggcagag ctggaatcct ggggcgttaa cacccgttac
     2881 accaaacggt ataaccaggc gaaatcttcg caatccgcca tcatggtgga taccaaaggc
     2941 gagcggataa tcattaacta ccccagcccg gatctgctgc ctgacgcaga gtggttggag
     3001 gaaattgatt tctctcagtg ggatgttgtg ctggcagatg tacgctggca cgacggcgct
     3061 aaaaaagcct tcaccctggc ccgtcaggcg ggtgtgatga ccgttctgga cggggacatt
     3121 acgtcgcagg atatcagtga gctggtggca ttaagcgatc acgcggcctt ttcagaaccg
     3181 ggtctggcgc gcttaacggg cgtgaaagag atggccagtg cgctaaaaca ggcacaaacg
     3241 ctcacaaatg gtcatgtcta tgtgacccag ggtagcgcag gctgcgactg gctggaaaat
     3301 ggtgggcgtc agcatcaacc ggccttcaaa gttgatgtgg tagataccac aggtgcgggt
     3361 gatgtttttc acggcgcttt ggcggtggcg ctggcaacaa gtggggattt agcggagtca
     3421 gtccgcttcg ccagcggtgt agcggcgtta aaatgcacac gtcccggtgg acgagccggg
     3481 atccctgact gtgatcaaac ccgatctttt ttgtcacttt ttgtataaaa tgccagggtg
     3541 atggtttttc gaggaatttt catgagcctt accgaactaa ccggtaaccc gcggcacgac
     3601 caactcctca tgctgatcgc cgagcgtggg tatatgaata ttgatgagct ggcaaatctg
     3661 ctggatgtct ccacgcagac ggtccgccgg gatattcgta aattaagcga gcaaggcctg
     3721 attacgcgcc atcacggtgg cgcgggtcgg gcttccagcg tcgttaatac ggcgttcgag
     3781 cagcgtgagg tttcgcaaac cgagaaaaaa aaagcgattg ccgaagcggt ggcagactat
     3841 attcctgatg gttcaacaat atttatcacc attggtacga ctgttgagca tgttgcccgg
     3901 gcgttactta accataatca tttgcggata atcaccaaca gcctgcgtgt ggcgcatatt
     3961 ctttaccaca acccgcgctt tgaagtgatg gtgcccggcg gtacgttgcg ctctcataat
     4021 agcgggatca ttggcccttc agcggcgtcc tttgtggctg attttcgtgc tgattatctg
     4081 gtaacaagcg ttggggcgat tgagagcgat ggcgcgttga tggagtttga tgtaaacgaa
     4141 gctaacgtgg tgaaaacgat gatggcgcac gcgagaaata ttctgctggt cgccgatcac
     4201 actaagtatc atgcttcggc ggcggttgaa attggtaacg tggcacaggt cactgcgctc
     4261 tttaccgacg agctgccgcc cgctgcgcta aaatcacgct tacaagacag ccaaattgaa
     4321 atcattcttc cccaggaaga cgcgtagatt tttgtgaccg ttaacctggc ttcatacctt
     4381 gccacatagc caaacccatc ctttcccgct acagttaatt tcttgtggcg cgaaaggagg
     4441 caaaaatgct ctatatcttt gatttaggta atgtgattgt cgatatcgac tttaaccgtg
     4501 tgctgggagc ctggagcgat ttaacgcgta ttccgctggc atcgcttaag aagagttttc
     4561 atatggggga ggcgtttcat cagcatgagc gtggggaaat tagcgacgaa gcgttcgcag
     4621 aggcgctgtg tcatgagatg gctctaccgc taagctacga gcagttctct cacggctggc
     4681 aggcggtgtt tgttgcgctg cgcccggaag tgatcgccat catgcataaa ctgcgtgagc
     4741 aggggcatcg cgtggtggtg ctttccaata ccaaccgcct gcataccacc ttctggccgg
     4801 aagaataccc ggaaattcgt gatgctgctg accatatcta tctgtcgcaa gatctgggga
     4861 tgcgcaaacc tgaagcacga atttaccagc atgttttgca ggcggaaggt ttttcaccca
     4921 gcgatacggt ctttttcgac gataacgccg ataatataga aggagctaat cagctaggca
     4981 ttaccagtat tctggtgaaa gataaaacca ccatcccgga ctatttcgcg aaggtgttat
     5041 gctaaaaacc attcaggaca aagccaggca ccgtacccgt ccactatggg cctggctaaa
     5101 actactctgg caacgtattg atgaggacaa catgacaacc ctggcaggta accttgccta
     5161 tgtgtcgttg ctctcattag tgccgctggt tgccgttgtt tttgcgcttt tcgccgcttt
     5221 tcccatgttt tccgacgtca gcattcagtt acgtcacttt atttttgcca actttctgcc
     5281 tgctactggc gatgttattc agcggtatat cgaacaattt gttgctaatt ccaacaagat
     5341 gaccgccgtt ggggcgtgcg ggctgatcgt caccgcgtta ttgttgatgt actccatcga
     5401 tagcgcgttg aataccatct ggcgcagtaa acgagcgcga ccaaaaattt actcgttcgc
     5461 cgtgtactgg atgattttaa cgctggggcc gctgctggca ggggccagtc tggcgatcag
     5521 ttcctatttg ctctccctgc gctgggcgag cgatctcaat actgtcatcg acaacgtgct
     5581 gcgtattttt ccgttgctgt tgtcgtggat ctccttctgg ttgctgtaca gcattgttcc
     5641 taccatccgc gtacctaacc gcgacgcgat tgtcggcgcg tttgtcgccg cactcctgtt
     5701 cgaagcagga aagaaaggtt tcgcgcttta tatcaccatg ttcccgtcat atcagctcat
     5761 ttacggtgtg ctggcggtga tccccattct ctttgtttgg gtctactgga catggtgtat
     5821 cgtcttgctt ggcgcggaaa ttactgtcac tctcggggaa taccgcaaac taaaacaagc
     5881 agctgaacaa gaagaagacg acgaaccatg attgcattaa ttcaacgcgt aacccgtgcc
     5941 agcgtcaccg tggagggaga agtgacgggc gaaattggcg cgggactttt ggtgttattg
     6001 ggtgtcgaaa aggatgacga cgaacagaaa gcaaaccgtc tgtgcgagcg tgtgctcggc
     6061 taccgcatct ttagcgatgc cgaaggcaag atgaatctca acgtgcaaca ggcgggcggc
     6121 agtgtactgg tggtttccca gtttaccctc gccgcagata ccgaacgggg gatgcgccca
     6181 agtttctcca aaggcgcatc accggatcgc gcagaggcgt tatatgacta tttcgtcgaa
     6241 cgctgccgcc agcaagagat gaacacgcaa acaggacgct tcgctgccga tatgcaggta
     6301 tcgctggtca atgatggccc cgtgacattc tggttgcagg tatgagccag cttccagggt
     6361 tttcacggga aacaagagag agtatcgcta tgtatcacct tcaggttcca caaacagaag
     6421 aagagttaga gcgttactat cagtttcgct gggaaatgtt gcgtaagccc ctgcatcaac
     6481 caaaaggttc ggaacgcgac gcgtgggatg cgatggcgca tcaccagatg gtcgtcgacg
     6541 agcagggtaa tctggtggcg gtaggccgat tgtatattaa tgccgacaat gaagcgtcca
     6601 ttcgctttat ggccgttcac ccggacgtgc aggacaaagg gttaggaacg ctgatggcga
     6661 tgaccctgga gtcggtggcg cgtcaggaag gcgttaagcg cgtgacctgt agcgcccgtg
     6721 aagacgcggt ggagtttttc gccaagctgg ggtttgttaa tcagggagaa atcaccacgc
     6781 caaccaccac gccgattcgc cattttttga tgattaagcc cgtcgccact ctggatgata
     6841 ttttgcatcg cggcgactgg tgcgcgcagc tgcaacaggc gtggtacgaa catatcccgc
     6901 ttagtgaaaa aatgggcgtg cgtattcaac aatataccgg gcaaaaattt atcaccacca
     6961 tgccggaaac cggcaatcag aatccgcacc atacgctgtt tgccgggagt ttattctcgc
     7021 tggcgacgct caccggttgg gggcttatct ggctgatgct gcgtgaacgc cacctcggcg
     7081 gaacgattat tctggcggat gcgcatatcc gctacagcaa accgattagc ggtaaacccc
     7141 atgcggtagc cgaccttggt gccttaagcg gcgatctcga ccgtctggcg cgcggacgaa
     7201 aagcacgggt gcagatgcag gtcgaaatct ttggcgacga gacgccgggt gcagtgtttg
     7261 aaggcacgta tatcgttctg cccgcgaagc catttggccc gtatgaagag ggcgggaacg
     7321 aagaagagta gcttatggag aggggcggtg ctgcctctct cattcattcc ggtcatttac
     7381 atcagtccct gactattccg gttatattga ataagaactt tataaatttt tattcttcgt
     7441 tggcaccata cgtgtcttgc tacatcactg ttcaggagca tttaaatatt atttgtagag
     7501 ctgagtggca attattgcag ataatgctta actcatcatc ttagttcagg tttgagcgat
     7561 ttttgatagt caaaaccccc tctggtgcca tacttttttc ataaatattt ttaaaatcta
     7621 tagctcacaa acattatgca aagataatgg gctggcatac attgtcagta tcattcattt
     7681 actgctctgt atgttatgaa tcattatgtt gtatgtttgc tcttaaaccg tgggaaggag
     7741 gctataatgg ttgccaatat taatctcatt aaacaagaaa gttattctgt tgttaattta
     7801 gaaaaacaac taatgagtaa taaatcaccg aagcaaacgc tattaaatca aatttcactt
     7861 tgccttaaaa tggaaggtga ggagcaactg tgcgattgtg ttaacgaact gaagcatggt
     7921 ttgtcgagta ataatgaata tgacctgttt aaaaatagag aaacctcact gtggaaacgc
     7981 aaagtaccag aaagcgcagc gctggcaatg acgggaacag tatataaatt atttggtaat
     8041 cgcttactca acgcccgcaa tcagttagat aaaaatctcc tcgaaatggc tatgcaaatg
     8101 gtttatggca aagatatggt gacaaagtcc ggggatatat tgattgatat acaattaaat
     8161 aaggatggca gtctccagag taagcactac acacttgatg taggtgaagt tataaacagt
     8221 tataatattg accctctttg tgaatgcttc aagtattaat caaacttact tacatgataa
     8281 aaatcctggt gacgttatta gcctgtatgg cactttagat aacattcccc ttgtagttaa
     8341 ggaatgcacg ggtaaaaatc atcaaattaa tgttaaagcc aaattgccac aagaacgaag
     8401 tgaaaaagtt gaggatatgt gtgaaagaat gaaaggtggc atctctatgt ttaaccacac
     8461 cacaaaaaca gctgataata tagagcataa tttgcagcta agttttctcg tgggttcacg
     8521 tccgtcggtc accaataaat actcagcaaa agatg
//
LOCUS       DADLSE010000155         8546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLSE010000155 DADLSE010000000
VERSION     DADLSE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.156"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            128..1534
                     /gene="fliD"
                     /locus_tag="JN692_003468"
     CDS             128..1534
                     /gene="fliD"
                     /locus_tag="JN692_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7265680.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     ALAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            1559..1969
                     /gene="fliS"
                     /locus_tag="JN692_003469"
     CDS             1559..1969
                     /gene="fliS"
                     /locus_tag="JN692_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7265681.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            1969..2334
                     /gene="fliT"
                     /locus_tag="JN692_003470"
     CDS             1969..2334
                     /gene="fliT"
                     /locus_tag="JN692_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7265682.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            2412..3899
                     /gene="amyA"
                     /locus_tag="JN692_003471"
     CDS             2412..3899
                     /gene="amyA"
                     /locus_tag="JN692_003471"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7265683.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(3933..4346)
                     /locus_tag="JN692_003472"
     CDS             complement(3933..4346)
                     /locus_tag="JN692_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7265684.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            4533..5738
                     /gene="yedE"
                     /locus_tag="JN692_003473"
     CDS             4533..5738
                     /gene="yedE"
                     /locus_tag="JN692_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7265685.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            5735..5968
                     /gene="yedF"
                     /locus_tag="JN692_003474"
     CDS             5735..5968
                     /gene="yedF"
                     /locus_tag="JN692_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7265686.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            6075..6745
                     /locus_tag="JN692_003475"
                     /pseudo
     CDS             6075..6745
                     /locus_tag="JN692_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN692_003476"
                     /pseudo
     CDS             complement(6785..7993)
                     /gene="tnpB"
                     /locus_tag="JN692_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
     gene            8001..8430
                     /gene="tnpA"
                     /locus_tag="JN692_003477"
                     /pseudo
     CDS             8001..8430
                     /gene="tnpA"
                     /locus_tag="JN692_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2086 a         2196 c         2180 g         2084 t
ORIGIN      
        1 gacgaaatac tcttttctct gccccttatt cccgctatta aaaaaaacaa ttaaacgtaa
       61 actttgcgca attcaggccg ataaccccgg tattcgtttt acgtgtcgaa agataaaagg
      121 aaatcgcatg gcaagtattt catcgctggg agtcgggtca ggtctggatt taagttcaat
      181 ccttgatagc ctcaccgccg cgcaaaaagc gacgctaacc cctatttcaa atcagcaatc
      241 gtcgtttacc gctaaactta gcgcctacgg tacgctgaaa agcgcgctga cgactttcca
      301 gaccgccaat actgcattgt ctaaagccga tcttttttcc gccaccagca ccaccagcag
      361 caccaccgcg ttcagtgcca ccaccgcggg taacgccatc gccgggaaat acaccatcag
      421 cgtcacccat ctggcgctgg cgcaaaccct gaccacgcgc accaccagag atgatacgaa
      481 aacggcgatc gccaccagcg acagcaaact caccattcaa caaggcggcg acaaagatcc
      541 gattaccatt gatatcagcg cggctaactc atcgttaagc gggatccgtg atgccatcaa
      601 caacgcaaaa gcgggcgtaa gtgcgagcat cattaacgtg ggtaacggtg aatatcgtct
      661 gtcagtcaca tcaaatgaca ccggccttga taatgcgatg acactctcgg tcagcggtga
      721 tgatgcgcta caaagtttta tgggctatga cgccagtgcc agcagcaacg gtatggaggt
      781 ctcggtcgcc gcccagaatg cgcagctgac ggtcaacaac gtcgccatcg agaacagcag
      841 caacactatc agcgacgcgc tggaaaacat caccctgaac ctgaacgatg tcaccacggg
      901 caaccagacg ctaaccatca ctcaggacac ctccaaagcg caaacggcga ttaaagactg
      961 ggtgaatgcc tataactcgc taatagatac cttcagcagc ctgaccaaat acaccgccgt
     1021 agatgcggga gctgatagcc agagttctag caatggcgca ctgctcggcg actccacgct
     1081 gcggacgatt cagacgcagt tgaagtcgat gctgagtaat accgtcagtt cttccagcta
     1141 taaaacgctg gcgcagattg gtatcacgac cgatcccagc gatggcaaac tggaactgga
     1201 tgccgacaaa ctcaccgctg cactgaaaaa agatgccagc ggcgtaggtg cattgattgt
     1261 tggcgatggt aaaaaaaccg gcatcacaac caccatcggc agcaacctga ccagttggct
     1321 ttcgacaacg ggcattatta aagccgctac cgatggcgtt agtaaaaccc tgaataagtt
     1381 aactaaagac tacaacgccg ccagcgatcg cattgatgcg caggtcgcgc gctacaaaga
     1441 acaatttacc caactggacg ttttaatgac ctcgttaaac agcaccagca gctacttaac
     1501 gcagcagttc gaaaacaaca gtaattccaa gtaatcatta ttcatcggga gacaggtcat
     1561 gtacgcggca aaaggcaccc aggcctatgc acaaattggc gtcgaaagcg ccgtaatgag
     1621 cgccagccaa cagcagttgg tcaccatgct atttgatgga gtgctgagcg cactggttag
     1681 agcgcgactg tttatgcagg acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc
     1741 gatcaacatc attgagaacg gactgcgggt gagtcttgat gaagagagca aagacgaatt
     1801 aacccaaaac ttaattgctc tttatagcta tatggtcagg cgtttgctgc aagccaattt
     1861 acgcaacgat gtctccgcag tcgaagaggt ggaagcatta atgcgtaata ttgccgatgc
     1921 ctggaaagag tctttactct ccccttcttc gattcaggac ccagtctgat gaacaatgca
     1981 ccgcatttgt atttcgcctg gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg
     2041 gcaacggaag aacaatggga cgaactcatc gctagcgaaa tggcgtatgt gaatgcggtg
     2101 caggagatcg cacacctgac tgaagaggtt gccccgtcca ctacgatgca ggagcagctc
     2161 cgcccgatgc tgcacctgat tctcgacaac gaaagcaagg taaagcagtt attacagatt
     2221 cggatggatg aactggcgaa actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt
     2281 gcctatggcg atcagggcgg ctttgtgctg gctccgcagg ataacctctt ttgaatctta
     2341 atgagccgat ggctcgcgaa taatccgatt acggctacgc ttctaatatt ccccttgaat
     2401 ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt tcactggtat tacccggaag
     2461 gcggtaagct ctggcctgaa ctggccgagc gcgcagacgg ttttaatgat attggtatca
     2521 atatggtctg gttgccgccc gcctataaag gcgcatcggg cggctattcg gtcggctacg
     2581 actcctatga tttatttgat ctaggtgagt ttgatcagaa aggcagcatc cctactaaat
     2641 atggtgataa agcacaactg ctggccgcca ttgacgccct gaaacgtaat gacattgcgg
     2701 tgctgctgga cgtggtagtc aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg
     2761 tgcagcgtgt aaatgctgat gaccgtacgc aaattgacga agaaatcatt gagtgtgaag
     2821 gctggacgcg ttacaccttc cccgctcgtg ccgggcaata ctcgcagttt atctgggatt
     2881 tcaaatgttt tagcggtatc gaccatatcg aacaccctga tgaagatggc atttttaaaa
     2941 ttgttaacga ctataccgga gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt
     3001 tcgattatct gatgggtgag aatatcgatt ttcgcaatca tgccgtgacg gaagagatta
     3061 aatactgggc gcgctgggtg atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg
     3121 tcaaacatat tccggcatgg ttttataaag agtggatcga acacgtacag gaagttgcgc
     3181 caaagccgct gtttattgtg gctgaatact ggtcgcatga agtcgataaa ctgcaaacgt
     3241 atattgatca ggtggaaggc aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc
     3301 atgaagcatc gcgcatgggt cgcgactacg acatgacgca gatcttcact gggacgctgg
     3361 tggaagccga tccttttcac gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc
     3421 aagccctcga agcaccggtc gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt
     3481 tgcgggaaaa tggcgttcct tcggtgttct atccggacct ctacggtgca cattacgaag
     3541 atgtcggtgg tgacgggcaa acctatccga tagatatgcc aataatcgaa cagcttgatg
     3601 agttaattct cgcccgtcag cgtttcgccc acggtgtaca gacattattt ttcgaccatc
     3661 cgaactgcat tgcctttagc cgcagtggca ccgacgaata tcccggctgc gtggtggtca
     3721 tgtcgaacgg agatgatggc gaaaaaacca ttcatctggg agagaattac ggcaataaaa
     3781 cctggcgtga ttttctgggg aaccggcaag agagcgtagt gacagacgaa aacggcgaag
     3841 cgaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa
     3901 tccatccccg gcggcaagcc ggggagattt cattacggca gtggcgtcgg taacggcgct
     3961 ttatccagcg cggcggcaca ctctgccgtc gggcgatcca cacgctccat cgtcatacca
     4021 tcgtactcaa tggtgttacc gtcacgctca acctcgtaca gctcacgttt tacggtaacg
     4081 ttagtgagat catcagacat caatgtcaac ttacccggca cggcaatcac ccgctgccac
     4141 tgacggcaat ccagggtatc gccctcttta gtgacaatca ggctgcctat cgcttccgga
     4201 ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc ccgccagcca gtccggcgcg
     4261 ggggttttca cgacattgtt atagttttcg acttcggcac agcccgccag caacatgagc
     4321 gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg
     4381 gcattaaagc tctgatgtcg ccagcctttc caggttcaga gagaaatttg atctaactat
     4441 tatttttgtg aattattacc ccgaaaataa atttgcatgg tgtatgattc gcggctcttg
     4501 tttcaaacct tctgagttca gaggctacat tcatgtcatg gcagcaattc aaacacgcct
     4561 ggttgattaa attctgggca cccatccctg cggtcatcgc ggcgggtatt ctctccactt
     4621 actattttgg cattactggc accttttggg ctgtcacggg tgaatttacc cgttggggcg
     4681 gtcagctcct gcagctgttt ggcgtccatg ctgaagagtg gggttatttt aaaattatcc
     4741 atctggaagg atcgccatta acccgcatcg acggcatgat gatcctcggt atgtttggcg
     4801 gctgctttgc tgctgcgctg tgggccaaca atgtcaaact acgcatgcca cgcagccgta
     4861 tccgcattat gcaggccatc attggcggca ttatcgccgg ttttggcgcg cgtctggcaa
     4921 tgggctgtaa cctggcggcg ttctttaccg gcattcctca gttctcgctg catgcctggt
     4981 tctttgccat cgccactgcc attggttcat ggtttggcgc gcgctttacc ctgctgccca
     5041 tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac
     5101 cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt ctttttcggc atgctgggct
     5161 gggcgctgct cacggcgatg aaccaaccaa aactggggct ggcaatgctg tttggcgtcg
     5221 gctttggttt actgattgaa cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt
     5281 ggatcaccgg acgtacccat atggcgaaag caatcattat cggtatggcg gtgagtgcca
     5341 tcgggatctt cagttacgta cagttaggcg ttgaacctaa aatcatgtgg gcgggaccaa
     5401 atgcggtaat tggtggttta ctttttggtt ttggcatcgt gctggcaggc ggctgcgaaa
     5461 ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca
     5521 acgtgatcgg ctcaacgatt ctggcgtatt actgggatga tttcgctccg gcgctggcca
     5581 ccgactggga caaaatcaac ctgctgaaaa cttttggtcc gatgggaggc ctgctggtga
     5641 catatttgct gctgtttgct gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc
     5701 gccgtgcggc accgcagact gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg
     5761 ccttgatatg gtgggtgaac cctgccctta tccggcagtc gcaacccttg aggcgatgcc
     5821 gcaattaaaa aaaggtgaga tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa
     5881 tattccactg gatgcgcgta atcacggcta cacggtgctg gatattcagc aagacgggcc
     5941 gaccattcgt tatttaattc agaagtaatc cctcattccc gctgtggccc acagcgggaa
     6001 catcgttacc tctgacacat agattttacc cgccccctct cctattcttt tcagtatgat
     6061 ctcaggaggt tgctatgtgt ggacgctttg cccaatccca aacgcgtgag gattaccttg
     6121 cgcttctcgc ggaagatatt gaacgcgata ttccctacga tcccgaaccc attggcagat
     6181 acaatgtcgc gccgggaacc aaagttcttc tgctcagtga acgtgatgaa caccttcatc
     6241 tggatccggt tttctgggga tatgctcccg gatggtggga taaaccgccg ctgattaacg
     6301 cccgcgtaga aactgcctca accagtcgca tgtttaaacc gctctggcaa catggtcggg
     6361 caatctgttt tgccgatggc tggtttgagt ggaaaaaaga aggcgacaaa aaaagcctta
     6421 ttttatctat cgcgctgacg gacaacctgt ttttatggcc gcgataggta gcataccgtt
     6481 tgagcgtggt gacgaagccg aaggattttt gatcgtcact gcggcggcag atcaaggtct
     6541 ggtagatatt catgaccgcc gcccgctggt tctgtcgcca gaagctgcac gggaatggat
     6601 gcggcaagac attggtggta aagaagcctc agaaatcgct accagaagct gtgtgccagc
     6661 aaaccagttc acctggcacc ctgtgtcgcg cgcggtgggt aatgtcaaaa accagggcgc
     6721 ggagttaatt caacctgttt gttgacatcc tccacgccct gaatgacgag gacccctgct
     6781 acgttcaggc tgtcgcctga atcatttcgg tgggttcctg cttcaacgag cggcctgact
     6841 gcaccatctc tccacaggca agaacggcgt gccccgccgc taaaatgtta cgagcgccat
     6901 ttacatcggc gttcgctgta tatccacata cctggcatct gaatttactt tgtgacaggc
     6961 gattttcttt cgctgtatga ccacagcacg cgcaacgctg gcttgtgtac gctggcggaa
     7021 cagcaagcac ctgaccgcca cgccagagct gcttgtactc aagctggcgg cgcatttcat
     7081 accagccctg atccagtatc gaacggttta aacctgattt tgcccggaca ttgcgccccg
     7141 gctggctgac cgtacccgct gctgactttg acatgttgct gaccttcaaa tcctcaatga
     7201 ctatcattac gtggtttttg ctgacggtcg ttgtgacttt gtgaaggtag tccctgcgga
     7261 tatttgcgat acaggaatgc agtcgctgta ttttgcgttt ctgcttctgc cagttgttgc
     7321 tgaatttgac cttgcggctt aactgtcgct gaagtctcgc cagcttcttc tggtttttct
     7381 gaaaactgtt tacaggctca aagactgtgc catctgacag cgaggcaagt ttagccacgc
     7441 cagcatccag cccgaccatt gatgctgaag ggtgaaccgg agttgatact tcactttctg
     7501 tctgaatact gatgtaccac ttaccgcagg actggctgac agtgacattt ttcacaacac
     7561 ccgtgacctg acggctgttc cggtagcgca tccagccaag ttttggcaga aaaatacggc
     7621 tgttttcctg agcgagctta acgccttgcg gatagcggaa tgcatcattc tgtcaccgct
     7681 ttttgaatca ggggaacgcc gaccgcttct ggaaaaagtt tttgcaggcc tgctcagggt
     7741 ctttcagtga ctgttgcaat ggctgtgacg gagcatcttt aatccattgc gtttcagtgg
     7801 catttttcca ctcaaccaat caggaagcca ttttcgcgta agggatgtct ttgttaccgg
     7861 ccttatgatt ctcattctga cgtgccagag tacgactgaa aacgaaacga catgcgcctg
     7921 cgaagcgcct catttcacgc gcctgttgac caccgggtct taaccggaat ttgaatacct
     7981 gtagtcgttt cacagggcaa attatacttt ggtctgtgaa aaaaactgat atccgacgcg
     8041 gaaggcactg tgttttcctg atgcatgctc accgggtctt tgttaccaag taccgacgcc
     8101 agatttttga ccatgacgcg acagaaaaac tacgtactta cttttcaaat gtatgtgctg
     8161 attttgaagc tgaactggtt gaaatggatg gcgaaccaga gcacgtccat ctgttaatta
     8221 actatccccc caaactggcg atatccagtc tggtaaacaa acagcctcaa aggcgtatcg
     8281 ggtaggttac tgcgacggga tcgaccagat attgctgcca ggtattaccg caaagatgtt
     8341 ttgtggcgtc ccggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
     8401 caatacattg aacaacagca aacacctggt caggtgaaaa aacgagcctt atatccccgc
     8461 cctgaaggac gggcttttac ggtgcactgg ataagcctgg agattatcta cacctgcatg
     8521 gacatcattg tactatgccc cgtaca
//
LOCUS       DADLSE010000156         8320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLSE010000156 DADLSE010000000
VERSION     DADLSE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.157"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1631
                     /locus_tag="JN692_003478"
                     /pseudo
     CDS             1..1631
                     /locus_tag="JN692_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN692_003479"
     CDS             complement(1600..3336)
                     /gene="aceK"
                     /locus_tag="JN692_003479"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7265687.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHCSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN692_003480"
     CDS             complement(3520..4824)
                     /gene="aceA"
                     /locus_tag="JN692_003480"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7265688.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN692_003481"
     CDS             complement(4854..6455)
                     /gene="aceB"
                     /locus_tag="JN692_003481"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7265689.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRSVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN692_003482"
     CDS             complement(6724..7653)
                     /gene="metA"
                     /locus_tag="JN692_003482"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7265690.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7810..8253
                     /locus_tag="JN692_003483"
     CDS             7810..8253
                     /locus_tag="JN692_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAY7265691.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2100 a         2050 c         2073 g         2097 t
ORIGIN      
        1 ctcactcact tcaacaaaaa atgcaaaaac cagactttgg cgttgatttc cagccgtcct
       61 gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac
      121 aagatggaat caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct
      181 ttgacggtcc ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg
      241 gtttatgctc ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat
      301 attaaacact attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca
      361 aatgatcatg ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta
      421 ttgatgttaa aaggaactgt ggatgaaata acccctccat tatcaccagc aacgatgaat
      481 ttgctaatgg caattggtca gaatcaccaa cttacgcaac tgatgattca gctccagaaa
      541 atgccagaac tacatagaac agaaatgttg actgcctata atagtattaa cctccccggt
      601 ttatatttgg ctataaatta tggcaatgcg gatatcgttg agactatttt caattcattg
      661 tcagaaacag gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca
      721 aaagataaaa atggtttttc tggattattt ttagcgatat cacgtaagga taaaaatgtt
      781 gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa cacatcattt agataatgaa
      841 caggtgtata aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt
      901 atggcgaatg gtgatgccga catgctgaaa attgttttgg tcgcgttacc tttgttaatt
      961 cgcacatgtc atttgactaa agaacaggta ctcgatctcc tgaaggcaaa ggatttttat
     1021 ggttgcccaa gactatacct ggcgatgcaa aatggacata gcgatatcgt gaaagttatt
     1081 ctcgaagcat tgccctgtct ggcccaggaa attaacattt cagcttccga tattgtagat
     1141 cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt ttatggccat gcagcgcgga
     1201 catatgaacg ttattaatac tatttttaac gcattaccca ctctttttaa tacgtttaaa
     1261 ttcgataaaa aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt
     1321 tgttttcagc gatacagcat aaacaacaaa acgttgtaga gacggtttat cttgctttat
     1381 ctgaccatgc acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca
     1441 aagcgccaca aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg
     1501 ctgaattaat ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc
     1561 ctcgatacat tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata
     1621 ccgtacgcta aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg
     1681 tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc
     1741 aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac
     1801 atcgcccggc gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg
     1861 cggcgggatg tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa
     1921 aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc cagggaaaat
     1981 gttagcagcg gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg
     2041 ctgaccttcc acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag
     2101 atggcgaatt acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag
     2161 taattccatt aataccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg
     2221 ggtgtccgcc attcggccca cacggtcgtg ctctttcacc agttgatagc aggcacgaac
     2281 gtgagcggca gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg
     2341 atcaaagccc ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat
     2401 gaactgctca ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc
     2461 gtgcttctgg cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc
     2521 ccgtagccac tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc
     2581 aaagccaaaa acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc
     2641 gccgtcgtcc gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag
     2701 tttgcccacc agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg
     2761 actttccgcg aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat
     2821 gtcacggctt ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag
     2881 tagagattcc cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg
     2941 aaagcggcgc tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcagtg
     3001 gtcaaataac cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta
     3061 atccggcaac agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact
     3121 ttggccgtta gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag
     3181 atggatacgg tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg
     3241 ctgcgccccg gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat
     3301 ggtttgagca atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag
     3361 aattgccgga tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat
     3421 gataagacgc gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtaaat
     3481 gccttatccg gcctacagtc agcaacgttt gtaggttgct tagaactgcg attcttcagt
     3541 ggagccagtc agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc
     3601 gaagtaacct gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc
     3661 ggcaaattcc ggctgctgca ctttctcaac atagtgcttc ataccttcgc cctgcgcata
     3721 agcgtttgcc aggtcgaaca tgttaaacca catgctgtgg atacctgcca gggtgatgaa
     3781 ctggaacttg tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc
     3841 gaggtttttc tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata
     3901 tttcgcgtgg atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc
     3961 acaccagact aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat
     4021 gcccgcatga gtacggaaga agccttcact ggtacgctcg ccggtaataa attcgctgtc
     4081 atacgggtcg caatctgagg tgatcagatc cgccgcatca gcatcggtac gtgcaaccag
     4141 cagggttgga acgcccgtca cgtcagctgc cagacgcgcc gcgaccagtt tctgaatagc
     4201 ttcctgagtt ggcactaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag
     4261 ctgatcttcg aagtgaactg ccgcagcacc ggcttcaatc atcgctttca tcagttcaaa
     4321 ggcattcagg acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc
     4381 gacatagcgc ggatcgcccg gctcaatgcc cacggaccat tggatctgat cggcacgacg
     4441 gaaggtgttg ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg
     4501 atccggatac atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata
     4561 gactgcttca atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag
     4621 gctgttgatg tagccttttt tcgactcacc gtgcagcaga cgccacattt tcgctgcgcc
     4681 cagttgcgcc agcgtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc
     4741 actgtatggg cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc
     4801 aatttgttgt gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct
     4861 aacaggcggt agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt
     4921 tccatcaagc gtgcggcatc gtcaaaacgg ccctgggaga aacgttcttc gcccagttcg
     4981 ctggcaatga ctttcatctc ttcgcccagc atctggcgga acaaggcttt ggtcaccggt
     5041 ttgccattgc tcaacgtttt ttgatgatgg atccactgcc agatcgaggt acgggaaatt
     5101 tcagccgtcg ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc
     5161 cacgcttcga tgtactgcac agccacgcga atgttggcgc gcataccttc ttcggtgcgt
     5221 tcaccatcac aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcaat
     5281 acttcaagct gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtgtcc
     5341 gcaaggcctg ggtgagcgat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta
     5401 tccgctttta ctttgttgag cacctggtta ttacgctctt catctttgct cggaataaac
     5461 gctgccatgc cgcccatcgc aaaagcaccg cgtttatggc aggttttaat cagcaggcgt
     5521 gagtaagcat tcaggaatgg tttatccatc gtcacggcct gtctgtctgg caggacacga
     5581 tcgggatagt ttttcaacgt tttgatatag ctgaagatgt aatcccagcg accgcagttc
     5641 agaccaacaa tatggtcgcg cagcgcgtga aggatttcgt ccatctggaa cacggcgggc
     5701 agcgtttcaa tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct
     5761 gcatagctga agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga
     5821 tagaaatagg gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc
     5881 gcaaaatcaa acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc
     5941 aagtgcagac cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt
     6001 ttgcctgctt cattggtata gctgatggta ccgttaaccg catcgcgcag gttaatttgc
     6061 ccgtcgatca ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact
     6121 ttcacattgg cgttcagtgc gttgatcacc atcttgcgct ctaccgggcc agttatctct
     6181 acgctgcggt cttctaagtc cgcaggaatc ccgcggattt tccaatcagc atcgcgaatg
     6241 gaagccgttt ccgaaataaa atcaggcaac gttccgttat caatatcttg ctgctgctga
     6301 atgcgcgctg ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga
     6361 aattctaccg cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttgtgaaa
     6421 gccagttcat cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggatccg
     6481 aaaacttccc ctctgtgaac gaaggaacgt tgtgcgcttt tcgttcagca caactaagac
     6541 tactcattta aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaat acttgataac
     6601 ttattgataa caatatgaat aaaatttatt cacttgctga ggtgcgtttc ggaaataaaa
     6661 aaggcacccg aaggtgcctg aggtaaggtg ctgaatcgct taacgatcga ctatcacaga
     6721 agattaatcc agcgttggat tcatgtgccg tagatcgtat ggcgtgatct gatagacgta
     6781 atagttgagc cagttggtaa acagcaaatt accgtgacta cgccagctcg ctcgcggttt
     6841 gttttgcgga tcgttgtgcg ggaaatagtt atacggtaca tccgggtcta gtccggcttc
     6901 cacatcgcgg aaatattcct gcgccagcgt ttgcgcatca tattcgggat ggcccgtcac
     6961 aaaggcaatg cgcttatctt tgctagcaaa cagatatgca tccccttctt ccgtctctgc
     7021 cagaatttcc agatcggtgt aatcacgaat caacgctgcc ggaaagtcag catagcgcga
     7081 atgcggtgcc agaaatgaat catcaaagcc acgcgtcaga agcgcatgag gatggagaat
     7141 atgatgctcg taaacgccag agagtttgtc ggtgcgagtt tgcttaggaa tgccgtagag
     7201 gatattgagc gcggcctgta ccgcccagca gacaaacagc gtcgaggtga cgtgatcttt
     7261 cgaccactcc agcacctgtt tgatctgcgg ccagtaagcg acatcattaa actccaccag
     7321 gcccagcggc gcaccggtta caatcagacc gtcaaagttc tgttcctgaa tatcttcaaa
     7381 gttacagtag aagttgttca gatgctctgc gggcgtgttg cgcgattcac gggaatcgat
     7441 gcgcaacagc tgaatatcga cctgcaaagg tgagtttgaa agcaggcgca gaaactgatt
     7501 ttcagtttca atcttcttcg gcatcaggtt aaggatcaga accttaagtg gacgaatttc
     7561 ctgaccagac gcacgagaag ttgtcatcac aaagacgttt tcttcacgca agaaattgac
     7621 ggcgggtagc tcgtccggca cacgaatcgg cataacctga ttacctcact acatacgctt
     7681 atacgtttag acatccagat agctgaagat aaccagaaaa tttgccaatg tcgagcctgc
     7741 atgttgaagg tgagaaagtt tcaaggaaaa ggctacgtta gaatataaga atgacgaaaa
     7801 ggagagaaaa tggttattag tattcgccgc tcacggcatg aggaagggga ggaactcgtt
     7861 gcgatttggt gtcgatctgt cgatgccacc cacgattttt tatcagcaga gtatcgggcc
     7921 gagctggaag agctggtgcg ttcctttctg ccggaagcac cgttgtgggt cgcggttaat
     7981 gaacgggagc agccggttgg gtttatgttg ttaagtgggc agcatatgga tgcgctgttt
     8041 atcgatcctg atgtgtgcgg ctgcggcgtg ggtcggatgc tggtggagca tgcgctctcg
     8101 atggcaccgg aactgacaac caacgttaat gagcaaaatg agcaggcggt tgggttctat
     8161 aagaaggtgg gttttaaggt tacgggacgc tctgaggtgg acgatttggg gaaaccgtat
     8221 ccgttgctga atctggcgta tgtgggggaa taagccgccc ctgccgtgcc tgcccaagct
     8281 ggagaagacg cgccagcgtc gcacccggca tttttttcac
//
LOCUS       DADLSE010000157         8167 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLSE010000157 DADLSE010000000
VERSION     DADLSE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.158"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1312
                     /locus_tag="JN692_003484"
     CDS             <1..1312
                     /locus_tag="JN692_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7265692.1"
                     /translation="DKVKYPTGVENHGGTLRIWFNFKGKRVRENLGVPDTAKNRKIAG
                     ELRTSVCFAIRTGNFDYAAQFPDSPNLKAFGVNKKEITVKELEEKWLDLKRMEISANA
                     FNRYESIARMMVPKIGGNRLVSAVTKEELLYIRKDLLTGYQNPTKGKAPVKGRSVVTV
                     NYYMTTIAGMFQFAADHGYIEANPFEGIKPLKRARAEPDPLTRDEFIRLIDACRHQQT
                     KNLWSLAVYTGMRHGELVSLAWEDIDLKAGTITIRRNYTKLGEFTLPKTEASTNRVVH
                     LIQPAISVLKNQAEMTRLGKQHNIKVQLREYGRSVNHECTFVFNPQVVRKSKQVGFIY
                     KVDSIGDSWETAIKRAGIRHRKAYQSRHTYACWSLSAGANPSFIASQMGHASAQMVFN
                     VYGAWMTDSNAEQIAMLNQKLADYVPMMSHGHQSDTRGLLKSVS"
     gene            1294..2064
                     /locus_tag="JN692_003485"
     CDS             1294..2064
                     /locus_tag="JN692_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7265693.1"
                     /translation="MKISELAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPAEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2117..2512
                     /locus_tag="JN692_003486"
     CDS             2117..2512
                     /locus_tag="JN692_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265694.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2553..3296
                     /gene="cmoA"
                     /locus_tag="JN692_003487"
     CDS             2553..3296
                     /gene="cmoA"
                     /locus_tag="JN692_003487"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7265695.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3293..4264
                     /gene="cmoB"
                     /locus_tag="JN692_003488"
     CDS             3293..4264
                     /gene="cmoB"
                     /locus_tag="JN692_003488"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7265696.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKEQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4429..6858)
                     /locus_tag="JN692_003489"
     CDS             complement(4429..6858)
                     /locus_tag="JN692_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7265697.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNTL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     MYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEACNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFCADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYTELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKSAVQA"
     gene            complement(6883..7983)
                     /locus_tag="JN692_003490"
     CDS             complement(6883..7983)
                     /locus_tag="JN692_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7265698.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1990 a         2067 c         1945 g         2165 t
ORIGIN      
        1 ggataaagtc aaatatccaa caggcgtcga aaaccacggt ggcacattac gcatctggtt
       61 taattttaaa ggtaaacgtg tcagggaaaa tcttggtgtc cctgacactg ccaagaacag
      121 gaagatcgcc ggagagctgc ggacatctgt atgttttgcc attcgtacag gaaattttga
      181 ttatgctgcg cagttccctg actctcctaa cctcaaggct tttggggtaa ataaaaaaga
      241 aattacggtg aaagaacttg aagaaaaatg gctggatctg aaacgaatgg aaatctctgc
      301 aaatgcattc aatcgctatg aatccattgc aaggatgatg gttccgaaga ttggtggcaa
      361 ccgactggta tcagcagtga ccaaagagga attgctgtat atcaggaaag atttgctgac
      421 cggataccaa aatccaacaa aaggtaaagc tccagtaaaa ggacgaagcg ttgtcacagt
      481 aaattattac atgacaacaa tcgctggaat gtttcagttt gctgcagatc acggttacat
      541 agaagcaaat cccttcgagg gaattaagcc tcttaaaaga gccagggcag aaccagatcc
      601 gctaactcgt gacgaattta ttcgcctgat agatgcttgc cggcatcagc agacaaaaaa
      661 cctgtggtca ttggctgtgt acacaggaat gcgtcatggt gaactggtct ccctggcctg
      721 ggaagatatc gatctgaaag caggaacaat tactatcagg cgcaattata cgaaactcgg
      781 tgagttcact ctacctaaaa ctgaagcaag tacaaacagg gttgtgcatc ttatccagcc
      841 cgctatcagt gtcctgaaaa atcaggctga aatgacaaga ctgggtaagc agcacaacat
      901 caaggttcaa ctacgtgaat atggacgttc agtaaatcat gaatgtactt tcgtatttaa
      961 cccccaggtg gttagaaaaa gcaaacaggt aggttttatc tacaaggtag attccattgg
     1021 cgactcatgg gaaacagcca ttaaacgtgc aggcatcagg catcgaaagg cataccagtc
     1081 acgacacact tatgcgtgct ggtcattatc tgccggagca aatccaagct tcattgccag
     1141 ccagatgggg catgcaagtg cccagatggt gttcaatgta tacggagcat ggatgactga
     1201 cagcaatgca gaacagatcg caatgctgaa tcagaagctg gcagattatg tcccaatgat
     1261 gtcccatggt caccaaagtg acacaagagg cttattaaaa tcagtgagtt agcttttaat
     1321 acccgtcatg ttaactgcgt ggagggcaac accacgcttt acgccctgcc gaaacccgag
     1381 gttgtcctgc gctggcgtga acagaccaca gatgacttcc gcttctgttt taagtttccg
     1441 gcgaccattt cgcatcaggc agcattacga cattgcgatg atttagtgac tgaatttttg
     1501 acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact gcctgccaca
     1561 ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc tgccgaattt
     1621 aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga ggaacaaacg
     1681 cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt
     1741 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
     1801 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
     1861 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
     1921 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
     1981 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
     2041 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
     2101 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
     2161 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
     2221 tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg aatatattcc
     2281 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaactt ggatggtgca
     2341 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
     2401 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
     2461 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
     2521 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     2581 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa gtcttcccgg
     2641 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     2701 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     2761 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     2821 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     2881 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     2941 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     3001 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     3061 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     3121 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     3181 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     3241 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     3301 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     3361 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     3421 caacgcggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     3481 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     3541 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     3601 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     3661 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     3721 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     3781 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     3841 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     3901 ttcgccgctg gagcatctct ggcagttgaa agaacaactg gtgaatgagg gtgaactggt
     3961 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     4021 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     4081 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     4141 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     4201 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     4261 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     4321 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     4381 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     4441 agcagactta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     4501 cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat ccgccgttaa
     4561 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     4621 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     4681 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     4741 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgtata
     4801 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     4861 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     4921 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     4981 cgcatttttc accggatcgg cgcagaaatc accataacga acgtactgtt cgttcttctc
     5041 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     5101 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     5161 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     5221 ggcaaacacg tcaaaatcgt tacacgcttc aaattgcgga gcaacagcct gcttcatcgg
     5281 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     5341 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     5401 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     5461 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacattt gttctttacc
     5521 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     5581 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     5641 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     5701 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     5761 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     5821 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     5881 ttccgccgcc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     5941 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     6001 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     6061 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     6121 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     6181 gctccaggca atttttaagg tattcagcgt gttcattccc cacagcacca caacctggct
     6241 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     6301 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     6361 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     6421 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     6481 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     6541 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     6601 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     6661 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     6721 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     6781 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     6841 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     6901 atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt cgctcatgct
     6961 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     7021 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     7081 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     7141 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     7201 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     7261 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     7321 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     7381 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     7441 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     7501 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     7561 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     7621 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     7681 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     7741 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     7801 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     7861 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     7921 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     7981 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     8041 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     8101 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     8161 tttatca
//
LOCUS       DADLSE010000158         7890 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLSE010000158 DADLSE010000000
VERSION     DADLSE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.159"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..518
                     /locus_tag="JN692_003491"
     CDS             <1..518
                     /locus_tag="JN692_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265699.1"
                     /translation="IIDIFLNALTTPIAQELLNKKLVMSILAMKIHDGEPGLYAAMEN
                     NHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNEDVVLSYI
                     STLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHIAIHHKHYKTVETYYAAINVISQSL
                     SFSADEIKTYL"
     gene            690..797
                     /locus_tag="JN692_003492"
     CDS             690..797
                     /locus_tag="JN692_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265700.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(850..1608)
                     /locus_tag="JN692_003493"
     CDS             complement(850..1608)
                     /locus_tag="JN692_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7265701.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1895..2824
                     /gene="pfkB"
                     /locus_tag="JN692_003494"
     CDS             1895..2824
                     /gene="pfkB"
                     /locus_tag="JN692_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7265702.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2925..3215
                     /gene="ghoS"
                     /locus_tag="JN692_003495"
     CDS             2925..3215
                     /gene="ghoS"
                     /locus_tag="JN692_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7265703.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3321..4181
                     /locus_tag="JN692_003496"
     CDS             3321..4181
                     /locus_tag="JN692_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7265704.1"
                     /translation="MWQAIIRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4222..4758)
                     /locus_tag="JN692_003497"
     CDS             complement(4222..4758)
                     /locus_tag="JN692_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265705.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4905..5573
                     /gene="hxpB"
                     /locus_tag="JN692_003498"
     CDS             4905..5573
                     /gene="hxpB"
                     /locus_tag="JN692_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HAY7265706.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     EQPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5736..6326
                     /locus_tag="JN692_003499"
     CDS             5736..6326
                     /locus_tag="JN692_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7265707.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6459..7850
                     /locus_tag="JN692_003500"
     CDS             6459..7850
                     /locus_tag="JN692_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAY7265708.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     DSFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         2030 a         1901 c         1915 g         2044 t
ORIGIN      
        1 aaataatcga tatctttctg aatgcattaa ccactccaat agcacaagag cttttaaaca
       61 aaaaactggt gatgagtatt ttagccatga aaatacatga tggtgagcca ggattatacg
      121 ccgcaatgga aaataatcac cctttgtgtg tcacacggtt cctctctaaa attaatggca
      181 tcgcctttaa atacaagttg agcaaagcta acatcatgga tttattaaaa ggagctacag
      241 cacagggaac ccctgcatta tacatcgcca tgagcaaggg taatgaagac gtcgtgttat
      301 cttatatatc gacgctgggt gcttttgcaa aaaaacattc ttttagtcaa catcagttat
      361 ttacactatt ggccgctaaa aatcatgaca acatgtcagc tgttcatata gccattcatc
      421 ataagcatta taaaactgta gaaacatatt atgctgctat aaatgtcatc agccaaagcc
      481 tgagttttag tgctgatgaa ataaagacgt atttataacc agctatttac gatatatatc
      541 gatacataat tttatttttt tcataaaacg cagaaacagt gaccatgtgc acaacgccac
      601 ccgacggctg taataacaaa aaatcccttg atgcctgtcc cttttgttac actccgttat
      661 cacgcacaag agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact
      721 ggaagatggt ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag
      781 caatgatttg gggctgagca gtgacaaacg aagacaacca gaccgcataa cacggtccgg
      841 cagataattt tacatgctgt agcccagcga taaggttgtc cgacggtcgg tatgttccgg
      901 tgcagactct ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg
      961 ttcattaata gcaacattta atgcgctttc ggagttcagc gttgtgtctt ccgcgccaaa
     1021 gaccgaaaca ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga
     1081 ggcatagccc agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc
     1141 tggaccgaat tcaaagcgga aactgtgtac cgggccattc aaaaactgac gaccataacc
     1201 cgcggtcaac acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg
     1261 accaaataaa tagtcataat cagttaaatt aaagcggcta cgcccgcccg ccgcatattt
     1321 ttctgaagaa cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc
     1381 agtggtttgt ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt
     1441 gcctgattgt gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc
     1501 aggatcgtcc atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat
     1561 gccccccgcc aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc
     1621 cgtacaacaa aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag
     1681 cgtccctgga aaggtaacga attataaaaa ggcgcgaata acttagcaat gtattcttat
     1741 ttcatttttt gaataagcat gtggcgaaaa cagattttta tttatatata tttatctgca
     1801 aaattttaaa taaagctcta ataaatcata ttgttaattt cttcactttc cgctgattcg
     1861 gtgccagact gaaatcagcc tataggagga aatgatggta cgtatctata cgttgacact
     1921 tgcgccctct ctcgatagcg caacaattac cccgcaaatt tatcccgaag gaaagctgcg
     1981 ctgtaccgca ccggtgttcg aacccggggg cggcggcatc aacgtcgccc gcgccattgc
     2041 ccatcttgga ggcagtgcca cagcgatctt cccggcgggt ggcgcgaccg gcgaacacct
     2101 ggtttcactg ttggcggatg aaaatgtccc cgtcgctact gtagaagcca aagactggac
     2161 ccggcagaat ttacacgtac atgtggaagc aagcggtgaa cagtatcgtt ttgttatgcc
     2221 aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa gagcaagttc tggaaattga
     2281 atccggggcc atcctggtca taagcggaag cctgccgcca ggtgtgaagc tggaaaaatt
     2341 aacccaactg atttccgctg cgcaaaacca agggatccgc tgcatcatcg acagttctgg
     2401 cgaagcgtta agtgcagcac tggcgattgg taacatcgag ttggttaagc ctaaccaaaa
     2461 agaactcagt gcgctggtga atcgcgaact cacccagcca gacgatgtcc gcaaagccgc
     2521 gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc gtttcccttg gtccacaagg
     2581 agcgctgggt gttgatagtg aaaactgcat tcaggtggtg ccaccaccgg tgaaaagcca
     2641 gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg acactgaaac tggcagaaaa
     2701 tgcctctctt gaagagatgg tccgttttgg cgtagctgcg gggagtgcag ccacactcaa
     2761 tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa atttacgctt acctttcccg
     2821 ctaacaaaaa cattccccca gcattggggg aatcaccacc aacctgtcgg caacgcgttt
     2881 ctccgactat gctcaaaagt catgtgataa caaaggggtg aactatggcc agtggcgatc
     2941 ttgtccgtta tgtcataacc gtcatgttgc atgaggatac attgactgaa attaacgagt
     3001 tgaataatta cctgactcgc gacggttttt tgctcaccat gacggatgat gagggaaata
     3061 tccatgagct ggggactaac acttttggac ttatcagtac ccaaagtgaa gaagaaatta
     3121 gagaactggt ttcggggctt acccaaagtg caaccggcaa agatcctgaa atcaccatca
     3181 cgacctggga ggaatggaat agcaacagaa aataaatggt ttttgggcaa taatcagtct
     3241 gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc gctaacctta tgatctggca
     3301 gacaacatgg gagagacatc atgtggcagg caatcattcg tcttttgagc gagcagttag
     3361 gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg agaagtccat gccgcatggc
     3421 atttgcgcta tgcaggacat gactttttcg tcaaatgtga tgaaagggaa ctgcttcccg
     3481 gttttaccgc cgaagccgac caactggagt tactgtcgcg tagtaaaacc gtcaccgtgc
     3541 ctaaggtttg ggcagtaggc gctgaccgtg actacagttt tctggtgatg gattatctcc
     3601 cacctcgtcc gctggatgcg catagcgcat ttattcttgg tcagcaaatt gcgcgtttac
     3661 atcaatggag tgaccaacca caatttggcc tcgattttga taactcgctc tccacaactc
     3721 cacagcccaa cacctggcaa cgtcgctggt caacgttttt tgctgaacaa cggattggct
     3781 ggcagttgga actggcagca gagaaaggga tcgctttcgg caatatcgac gccatcgtcg
     3841 agcatattca gcagcgtctg gcctcgcatc aaccgcagcc ttctctgttg cacggcgatt
     3901 tatggtccgg caactgtgcg ctgggtccgg atggcccgta cattttcgac cctgcctgct
     3961 actggggtga ccgagagtgc gacctagcga tgttaccgct gcatactgaa cagccgccac
     4021 aaatctatga cggctatcag tcagtatccc cgctacctgc cgatttcctt gaacgtcaac
     4081 cggtttacca actctacacg ctgttaaatc gtgcaagatt atttggcggt cagcatttgg
     4141 ttattgctca gcagtcattg gatagattat tagcagcatg atatgggttg aggataatgg
     4201 ccgctccgtg cggccttttg attaaataaa cccaagcaga gaaaagaaca cataacctgc
     4261 agcaatacaa ataaccggca ggatatatag tgagaaaaac tgcaggaaaa tagtatggtg
     4321 cggaacaaca atacgggact caatttgttc acgcgttagc ccctcttccc ctttggcttt
     4381 ttccagaatg agttgatctt caacaccttc ccgcaagaag cgcgcctggc gactcatccg
     4441 ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg atgaaaatca cccagaacat
     4501 aacgttcaca ccaccattaa aattaggggt cggggagtta taccagaaca gattcaaaaa
     4561 aggcgtattc gcctgcatca tatcgatcat gacatgagca aagtcgagca tgaccgcatt
     4621 aatgccttcc tggttttcct gccgggtatt cataaatttc agcaaagaaa tcaatgtaga
     4681 gatcaatgca ggtataaaaa tcacccaacc caaaatcctt ttcaaaacag caatgcgtcc
     4741 agcttgttga tacgtcatga gttctccttg attaagacgc gtcgtttcac ttagtttacc
     4801 tgtagatatc tgttttcgcc cattctttaa aggcgatatg ataggcgctt aatcataagc
     4861 acggcttaat accttacaca taatgctcta aaggagaggt tgtaatgtca accccgcgtc
     4921 agattcttgc tgcaattttt gatatggatg gattacttat cgactcagaa cctttatggg
     4981 atcgagccga actggatgtg atggcaagcc tgggggtgga tatctcccgt cgtaacgagc
     5041 agccggacac cttaggttta cgcatcgata tggtggtcga tctttggtac gcccggcaac
     5101 cgtggaatgg gccaagccgt caggaagtag tagaacgggt tattgcccgt gccatttcac
     5161 tggttgaaga gacacgtcca ttattaccag gcgtgcgcga agctgttgcg ttatgcaaag
     5221 aacaaggttt attggtggga ctggcctccg cgtcaccact acatatgctg gaaaaagtgt
     5281 tgaccatgtt tgacttacgc gacagtttcg atgccctcgc ctcggctgaa aaactgcctt
     5341 acagcaagcc gcatccgcaa gtatatctcg actgcgcagc aaaactgggc gttgacccac
     5401 tgacctgcgt ggcgctggaa gactcggtaa atggcatgat cgcttctaaa gcagcccgca
     5461 tgcgttccat cgtcgttcct gcgccagaag cgcaaaatga tccacgtttt gtattagcaa
     5521 acgtcaaact ctcatcgctg acagaactca ccgcaaaaga ccttctcggt taatgaccag
     5581 gggcagtgat cacgctgccc ctggttcttt atctgaattt gcattcaact gacggattaa
     5641 tcgtcaattt aagagaaaga gttacaccgt caccacttcc gtgcactgta taaaaatcct
     5701 atactgtacg tatcgacagt ttagtgagtt ttatcatgac ggcggaaggt caccttctct
     5761 tttctattgc ttgtgcggta tttgccaaaa atgccgagct gacgcccgtg ctggcacagg
     5821 gtgactggtg gcatattgtc ccttccgcaa tcctgacgtg tttgttaccg gacatcgatc
     5881 acccaaagtc gtttcttggg cagcgattaa aatggatatc aaaaccgatc gcccgcgctt
     5941 ttgggcatcg tggttttacc cacagtctgc tggcggtatt tgcgctgctg gcaacctttt
     6001 accttaaggt tccggaaggc tggttcattc cggctgatgc gctacaagga atggtgctgg
     6061 gttatttgag ccacatactt gccgatatgc tgacacccgc cggtgttccc ctgctctggc
     6121 catgccgctg gcgtttccgc ttgcctatcc tggttcccca aaagggcaac caactggaac
     6181 gttttatctg catggcatta tttgtctggt cggtatggat gccccattca ttacccgaga
     6241 acagcgctgt tcgttggtca tcgcaaatga tcaatacctt gcagatccag tttcatcggc
     6301 ttattaagca tcaggttgaa tactaaaaag gcaaaaatca cctttctgga ataagcaatt
     6361 ccatttgaat ataagagcca gctcacagtt ctgttaatct tgcgccaaca ctatgactgc
     6421 tacgcagtga tagaaataat aagatcagga gaacggggat gaactttcca ttaattgcga
     6481 acatcgtggt gttcgttgta ctgctgtttg cgctggctca gacccgccat aaacagtgga
     6541 gtctggcgaa aaaagtgctg gtgggtctgg tgatgggtgt ggtttttggc cttgccctgc
     6601 ataccattta tggttctgac agccaggtac ttaaagattc tgtacagtgg tttaacatcg
     6661 ttggtaacgg ctatgttcaa ctgctgcaaa tgatcgttat gccgttagtc ttcgcctcta
     6721 ttctgagcgc ggttgcccgt ctgcataacg catctcagtt aggcaaaatc agttttctga
     6781 ccatcggtac gcttttgttt accacgctga ttgcggcgct ggtcggtgtg ctggtcacca
     6841 acctgtttgg tttgacggct gaaggtctgg ttcagggtgg tgcagaaact gcacgtctga
     6901 acgccattga aagtaactat gttggtaaag tctctgatct gagcgttccg cagctggtct
     6961 tgtcctttat cccgaaaaac ccgtttgccg atcttaccgg agccaatccg acgtcaatta
     7021 tcagcgtggt aatttttgcc gcattcctcg gcgtagctgc attgaaactg ctgaaggatg
     7081 atgcgccgaa aggtgaacgc gtcttaaccg ctatcgatac cctacaaagc tgggtgatga
     7141 aactggttcg ccttgtcatg cagttgaccc cttacggcgt tctggctcta atgaccaaag
     7201 tggttgcagg ttctaacttg caagacatca tcaaactggg aagtttcgtt gtcgcgtcct
     7261 acctcggtct gctgattatg tttgcagtgc atggcattct gctgggcatt aatggcgtga
     7321 gtccgctgaa gtacttccgt aaggtatggc ctgtgctgac gtttgccttt accagccgtt
     7381 ccagtgctgc gtctatccca ctgaatgtgg aagcacaaac gcgtcgtctg ggcgttcctg
     7441 aatccatcgc cagtttcgcc gactctttcg gtgcaaccat tggtcagaac ggctgcgccg
     7501 gtttgtatcc ggcaatgctg gcggtgatgg ttgcgcctac ggttggcatt aacccgctgg
     7561 acccgatgtg gattgcgacg ctggtcggta ttgttaccgt tagttccgca ggcgttgccg
     7621 gtgtcggtgg tggtgcaact ttcgccgcac tgattgtgct gcctgcgatg ggcctgccag
     7681 taaccctggt ggcgctgtta atctccgttg aaccgcttat cgacatgggc cgtacggcgc
     7741 taaacgttag tggctcgatg acagctggca cgctgaccag ccagtggctg aagcaaaccg
     7801 ataaagccat tctggatagc gaagacgacg ccgaactggc acaccgttaa ttcttatgct
     7861 ggcaacggtc cgttttgtat aggggccgtt
//
LOCUS       DADLSE010000159         7859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLSE010000159 DADLSE010000000
VERSION     DADLSE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.160"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            4..435
                     /locus_tag="JN692_003501"
     CDS             4..435
                     /locus_tag="JN692_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265709.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..994)
                     /locus_tag="JN692_003502"
     CDS             complement(467..994)
                     /locus_tag="JN692_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7265710.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(987..1733)
                     /locus_tag="JN692_003503"
     CDS             complement(987..1733)
                     /locus_tag="JN692_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7265711.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1748..3655)
                     /locus_tag="JN692_003504"
     CDS             complement(1748..3655)
                     /locus_tag="JN692_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7265712.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4252..4644)
                     /locus_tag="JN692_003505"
     CDS             complement(4252..4644)
                     /locus_tag="JN692_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7265713.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4641..6842)
                     /locus_tag="JN692_003506"
     CDS             complement(4641..6842)
                     /locus_tag="JN692_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7265714.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(6839..7147)
                     /locus_tag="JN692_003507"
     CDS             complement(6839..7147)
                     /locus_tag="JN692_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265715.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7123..7458)
                     /locus_tag="JN692_003508"
     CDS             complement(7123..7458)
                     /locus_tag="JN692_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265716.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7485..7802)
                     /locus_tag="JN692_003509"
     CDS             complement(7485..7802)
                     /locus_tag="JN692_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265717.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
BASE COUNT         1641 a         2170 c         2144 g         1904 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagatac gtcacggcgg
      721 tgtagcggtc gctgttcgcc ttttcgtcgg ccaccacgcg cacggccacg ccgcgccggt
      781 tagcaccggc gagcgccgtc gcgaccggct tactggtgaa gctgtaggcc gcgacgtcaa
      841 tgctctgcct ggcgttgttt atggcgccga gcacgatttc aagagcaggg cgcccgtcag
      901 acggcgaaaa acccgccgtc aggacgggcg cggcggtggc gaacgcgggc acgcagaggg
      961 cggcggccag caccagaagg gtggttttac gcatagttgt ccagctccag gtttttcagt
     1021 tcatcgaggg tgtaaaagcg gaaaacgtcg cggtgttttg cctccagttg cacatgctgg
     1081 tttttcacga tcacgctggc gatgctgtcg aaaataatct tcagggcgcg ctttttcgcc
     1141 tcgtccgggg cgacgtaaaa cgcgtaccgc cattttttat cgctgatagc cagcaggtgg
     1201 ctcgccatga tggactgata gcgcgccttt gttttgatac gtcgttcggt ttcaacggcg
     1261 atcagcctgc cgtccggcag gctgataagc ccgtcgggac ggtgtttgac gctgtactga
     1321 ctgaggaacg ttgaacggtc gccgttaatc caccctgtcg cgccgttctt ctccagcgta
     1381 aggcgcacta gctggttatc aaggtgatgg tcgagtgacc agccggtcag gcggtagggt
     1441 tcaaagcggg cgggcattat gttgtcgtcc gccgtgagga taacggccag cccgtcggtg
     1501 gtaatgcccc acagggatat tctcgacgcg cgggattcat gcacgtgctt ctgaatcagc
     1561 cccattttct ccaccttctc caacaaccgg tagagagggc ggtcagattt aaaatcaaaa
     1621 agcagcttta tgatttgaaa atcgctgtag gtttcctcct tgagaaaatt cagcagtacg
     1681 cgaatttttt cagtgctgcg tgcataacgt tcctgatacg acgaaataag catagaaact
     1741 ccttatttta taaatccagc aacgaatgta tttcgtcatt tggagcgggc tgtgatgcca
     1801 tcagccactc ctcgtcaggt tgcaccggcg gagtgggtat tggcgttgct gagggttttt
     1861 tggtggttgt tgcgctgacc ggaataatgt caggtgccgg aactgccgga gccgttttat
     1921 caaaatccag atccagcacc ggtttaactt ctgccaccgt agcgactgcg accggcggca
     1981 gggtaaacag ctccagtggg gcttttttaa ccttgagcgg tgagattaac gacgggaacg
     2041 cgagcgcgtt ctggctgaag atatagctga cgaacttcgg caggttctgg agcatattgc
     2101 tgtcgatgta atagctttcc gcctgccgaa gcatgcgctc gttatcgact ttttctgtca
     2161 gcaccgccgt ggtggtgact ttccggcttt catcatcgac taaaatcgtg cccgacatgc
     2221 gcgcaatcca ttcggcggtg tctggatcct gaagtttata taccagctta aacttgcagt
     2281 tttccacaac cgccccaacc acggcgtcgc cgtttaaatc cgccggacag tcgcgcaggt
     2341 cggcaattga ctggtgcgcc attaatatat ggacgccttt atcgcgtgcc gcgcctaacc
     2401 cttcgagcgc cggtttggac aggtgatatt tcacctcgtc cttaaagatg gcgatggggc
     2461 ggatattccc cgcgacgcgg tcgcgcaatt ccgcgagctg gaacaggcga actaaaatca
     2521 tgcgctgcgc ggtaatgatt ttagagttac gcatcgaacc gatgatatag cagcagccgc
     2581 ccttgtcgaa aatctccttc agcgcaaaac cgcctggcgc gttaatgctg ttgagcattg
     2641 ccagctcctc ggttttaccg tgaaaggctt taatcgtctc gccaatgccc tgaacataat
     2701 cgccgttata aatatcccgc acggttgcgc ccggattatc cttcacgaac tgcgccgccg
     2761 ttctcgccgc cttgcggtcg tcgatacggt aaaaatccgc cgcctcgcct ttttccgcca
     2821 gactgaaacc ggccacgaat aattcctcca gatggtcggc ggtgataccg tcgataaggt
     2881 tcatctggta ttcctgttta ttcaggtcga taagcacaaa gggttttccg gcgtccgcgc
     2941 acgctttttt atacaggtgc ggtgcccact cgtcgttttt ggggtcgaga acaaaaacgc
     3001 cctcgtcggc gaggatgctc tgataaagca gaataccggt tacgacgcct ttaccggcac
     3061 cagtggtgcc gataatgtcg gcgtgctggg tctgccattc ttttaacgga atatattgcg
     3121 gctcaccctc gcggctcagc ccgataaaaa tccccttatt cagatcgatg taatccatcg
     3181 gatcgtattc tgtggtgacg ggtaaaattt cttttacccg gcgaacgtcg gttctgcggt
     3241 cgcgctccat gctggttttt ttcaccatgc cgcatttgac cttatcaaat tcaggcgaga
     3301 tcaaacgccg taaaaccacg tgcagaataa aaccggcgac ggtaaacagt agcgccattg
     3361 tccaggtaag cagcggcgga ctggcgatat cttcccggcc ataaatgcgc tgtaaaaacc
     3421 agacggccac tggcgctatc gagccgaaaa caaaacagat catggaaagc agaattaaga
     3481 tcctttgcag aaacgtcggc ttttcctgac cgccgtgttt cgcagaaaat ataaaaggca
     3541 gcatgacgcc ggacagcagc gctaacgcca gttgatgcgc ctgcaaaaag gcgataaagg
     3601 ccaggacggc atttgctatc ggcgacagca aatgattcag tttgtcgaat atcacgatat
     3661 tctccttcac ttaaattcgg tcagcaccat cggtcagtca gaacggtcac aggttgtgat
     3721 cgttctgact gaccgattcc atgctgcaaa agaagtggcc gctaagcggc catgatcgca
     3781 ggaggtacac tcccgcgatc taacggcgtg cggcattacg cgcgggctgg acgcccttac
     3841 tgcatgtcgc atgggggtaa tgtcctggct gaacgcccgg ccaaaacccc caaggtcaaa
     3901 agaggccaca ccgtcgcgcc acgctaccgg cttttggccg gagacttagg cacgacggtg
     3961 tggggggcaa gccccccaaa taaaccgacg cgtgggcgcg tctttttgct cgctggggcg
     4021 gcaaaattta ttcggccccc cccggctttt ttcggctcac ttggggtgag cgctcaaaaa
     4081 gtggaatcgg cgacgcaaat tagcaatttc ttcgaaattg aatttactcg cctcctgcca
     4141 cttcgcagac tcaggcggcc tgtcgattag caattttttc aaaattgaat cgccatgctc
     4201 gccagtcttc gaagcccgaa aagccaggtc aaaaccgacc ctgtgccgtt gttagtcggt
     4261 ggtaataagg aaggattcaa gaggttttcc ggcgtcaagt tcttgctgaa ttacaaccgg
     4321 cacgcgtccg acgcctgacc attttttatg cacgccgttt tcaaaatact gatatttagc
     4381 tttgcgtttt gccgactttt tggcgggagt tgtactaacc agctctgtcg gtgaaaagcc
     4441 ctccgattca atcagcgcca gcaattcatt gcgcttttct tcccgctgtc ggcgtagctt
     4501 ttcctgctcg atatgctctg cgcgtttttc atcaagcgct gctgaaatac gtgcactcag
     4561 catctccaga aaggcaaaat cactttcacg taaaaacttt ttcagcgcgc gctctgaggt
     4621 taattcgtcc tggatacggt tcataatttc agctccttcg gcatagatac gttcaatcct
     4681 ttcagcggat cgcgtggtgg atattcaaaa ccgagttccc cggtttgcgc gtttttcagc
     4741 gtgacgatca tcgaataggt gtcgtcgttt ttcttcgatt taaaaccttt aataacgtcg
     4801 gttttgccct tgccgattaa cttctcggcc tgtttttccg tcagcttttt gccgcgcact
     4861 tccagccata ttttgaactg acagcccgta caggcgcagg ttttcggcgt cataaccagc
     4921 tcagaaccgc acgacggaca tttaaccgcg aggcggttaa gcgccgtttt aacgggatgt
     4981 ttttccgtcg cgctgatggc aatatcggcg ctctcaacca ggcgggaaac ttccgcgtaa
     5041 agaccatcaa taaagcgctc gatggtcatt tcgccggttt caaccgccgc ctgtttttca
     5101 gcccatacgg cggtcatatc cggctgcgtc accgagtcgg gcagggcatc aattaacgcc
     5161 agcccggcgg cagttgggat aaatttgccc ttttccacgg tgatgtaatc gcgggttttc
     5221 agcgtctcaa taatagccgc gcgggtcgcc ggtgtgccga tgccgccgtg ttcatctttt
     5281 ttgtctttat ccttctcctt gagcaatttt ttaatgcgcg gatcggcgac aaaatcggcc
     5341 acgcgaacga gggcagcgag cagggtcgcc tcggtaaaca gcggcagcgg tttagttttt
     5401 ttctccgaaa tcaccacctc gcggcaggtt aatgtcgccc cggtttgcag gcggctgatg
     5461 gcctcaaacg aggtgctgtt ttcgtcctcc tcgtcgtctt cttccgcgtc gttcaggaac
     5521 gtcgtaaaac ccgcatcggt gattttacgc gccgagactt taaacatctc gtcaccgcat
     5581 ttaatctccg cgatcgactc gtcatagcgc tttttcgcca cgaactgcgc gagataaaac
     5641 tgcgcgatgg tgagataaac cgccttttcc ttgtcggtaa ggcggcttaa atccggcatg
     5701 ttcgtcgtcg ggataatggc ggtgtgggcg gtgacttttt tactgtcaaa cgccgcactt
     5761 ttttgcgccg tgtcggtggt aaggccgtta aaaacgtcga gcgtttttaa ggccgccacc
     5821 acctgtggcg cgtcgttgaa ttgttcatcg gacagataag aacagtcaga gcggttgtag
     5881 gtaatgtatt tactgtcctt gagcttctgc gtgatatcga gcgtctgctg cgcggtcagt
     5941 ttgtgcctgc ggttcatata cacctggagg cgcagcaggt taaacggcag cggtgcggcc
     6001 gtggtttttc cccggtcggt ggcggccgcg agcacgctgg ccggttttcc ggccagagcc
     6061 ctcgcgagtc cttcccccca ggccttatcc gtcagccgct ttttatcgtc caccggcgcg
     6121 tattcggacg tctgccagcg ggcgcagaca acgtccgtac cgctgataaa ctgccctgcg
     6181 agctggtagt aaaacgcctc gctgtgcgcc tgattgtcgc gccaccggcg cacaatcatt
     6241 cccagcaccg gcgtctggac gcggccaacc gaaatcgtgc ccttatagcc cttatcgcgg
     6301 gctttgaggg tgtaacagcg gctcatactg aagccaaaaa tcgcgtctcc tgcgctgcgc
     6361 atcagggctt tcaggtacat cccacgaaaa tcgctgttat cgcgcaggga gttgatggct
     6421 tttttcaccg ctggcgcggt gttgtcgtta ataagcgccc gtttcaccgg cgcggtgttt
     6481 ccggcgaact ccagcacctc gtcaaccagg agctgcccct cgtcgtccgg gtcgccgcag
     6541 tggacaacca ttttgatatc cgggcgccga atcagcttca ccagggtatt agcctgcttt
     6601 gccacgctcg cttttggctg aagctgtacc gggtacagct tcagcggcag ggtgtcgagc
     6661 gaccaggtgg cgtaggccgg gttgatcgtc tccgggggca ccatttccag cacgtggccg
     6721 acgcagtagc tcacgatatc ggtcgccgat tcggcatagc cgtcgtgccg ggtgaagttg
     6781 ccgcccagcg ctttcgcgat atcgctcgcg actgcgggtt tttctgcaat ccacagtctc
     6841 attcttgatc tctcctgatt tcatccatcg tttcgaagta catcattcga ctttcctgaa
     6901 agtcgttatc agacagacta aagccatgat ccgccgctac tgtcatgaac gtctcagcat
     6961 cacggacgct gagactggtc agaacatcgt gcaattcgca cagggcgcga tattggtaga
     7021 tttcgtaatg caggccgcag ccgcgatgcg cttctttctc gatgcgagaa atcaggctcg
     7081 cggcactaac ggaatcacca cgcacatgcg gccacggcgg ggctataaat ccggtttttg
     7141 ttgccattgg agtgcatcct tatcgttgag tttttcccat tccgccgcct cgtcgtcgct
     7201 cagcggaacg gtgtaaaaga agaccggcat actgagcacg gagcggaaga acgactgacg
     7261 ttctgctggt aatgcctgga aggtcggcga gtgctgcgcg gcgagctgga cgagttcgga
     7321 cgtgttggcc tgaaaatgat cctgtttctg gctgatttcc gacaaaacga gcagcaccgt
     7381 ctgcaaatca cctgcaggaa gctcctcatc tttcagcagc ccggaaaagt tattaataga
     7441 aacggtttga ttaatcacgt ggtgtctcct ttacggctgc cgttttacca accgttcata
     7501 cggccaaatt tcgagatgat cttgccgaca atggcgctgt cagcggacgg gcaggcgctc
     7561 agttttttct gccgcgctgc ggcggtgtga tcgggtaaaa acgacccctt ttttttcttt
     7621 ttgatgctca tgaaattttt gtacatcggg tcacactcgg cgggactgct gccgtcgagc
     7681 tttccggcca ggcacagcac catcgcgcac gggtcattcg ggtcggcatt ctggccatcg
     7741 gcggcgaacg ccggagaagt aagggcgcac agcagcgccg ccgtgattac ggtgtgtttc
     7801 atgatgattt cctctgaagg gaagcagggc gctaacgccc gttggttgtt caacgtcgg
//
LOCUS       DADLSE010000160         7814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLSE010000160 DADLSE010000000
VERSION     DADLSE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.161"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            179..397
                     /locus_tag="JN692_003510"
     CDS             179..397
                     /locus_tag="JN692_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7265718.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            615..857
                     /locus_tag="JN692_003511"
     CDS             615..857
                     /locus_tag="JN692_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265719.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN692_003512"
     CDS             complement(1187..2116)
                     /gene="fdhE"
                     /locus_tag="JN692_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7265720.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN692_003513"
     CDS             complement(2113..2748)
                     /gene="fdoI"
                     /locus_tag="JN692_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7265721.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTNAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN692_003514"
     CDS             complement(2745..3647)
                     /gene="fdxH"
                     /locus_tag="JN692_003514"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7265722.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN692_003515"
     CDS             complement(3660..6710)
                     /gene="fdnG"
                     /locus_tag="JN692_003515"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6123..6125),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7265723.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTETYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6904..7737
                     /gene="fdhD"
                     /locus_tag="JN692_003516"
     CDS             6904..7737
                     /gene="fdhD"
                     /locus_tag="JN692_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7265724.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1808 a         2004 c         2077 g         1925 t
ORIGIN      
        1 tcctgggcga taactaaacc atttttgagt aataaatgaa acaactgtaa cgctcttatt
       61 atttctggaa aacgcctcgc aaaaacaaaa tgacatgcaa aaatccgctt tttttaaatg
      121 cttttccggc gtaattttcg cacatacatt aatgagtaat atatgtaagg tggtggtaat
      181 ggcgatgaat acggtttttc ttcatttatc agaggaggca attaaacggc tgaacaagct
      241 tcggggctgg cgtaaggttt cacgatctgc aattttacgc gaagcggttg agcaatatct
      301 ggagcggcag caatttccgg tgcgtaaagc aaagggaggc aggcaaaagg gggaggtagt
      361 tggtgttgat gatcagtgta aggagcataa ggaatgaaaa aaatttttca ttcttgaata
      421 taaaaaacag atgcccttat tctggtatta atacaaggct gttttacttg aacttataat
      481 aactgcaact gttacatcgt atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa
      541 atgagctttc tttaaagatt cggcaagggt acttttctct ccatagattc aaaaatgata
      601 ttggcgagat atttatgaac tctctggcag gtatagatat gggcagaatt ttactcgatt
      661 tatcgaatga ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg
      721 cggacctatt aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa
      781 ccagtgttcc tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc
      841 tgcgcgagga atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt
      901 tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat
      961 atattgaagt ttattggtag gcctgataag cgcagcgcat caggcaatca tacgcttgtc
     1021 atcagtgaca tacccataac cttatcgatg tgttggaggg aagaacgagg caaacagaca
     1081 attgcggcat gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg tcacacccta
     1141 tgagctgtaa tccgggcgac ctgcgccacc cggaaaacgt cagagattac tctccttcac
     1201 ccggaaacag gaacgggtta atggaactac gggcgtagcc ttcttgctcc attcgcgcgt
     1261 ccagtaccag agaggcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat
     1321 aaagaatctt caggtaagtg ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt
     1381 cgtcatccag cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc
     1441 gcactacgtg ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg
     1501 tgccaatttg caccatgctg gacaccggca tagagccaca taccgggcaa tattgacgtt
     1561 gttcgccgta ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga
     1621 gcgacagcgc agcccagata aacggcgctt tatcgctgct gacggacgag aaatcagagg
     1681 caaacagcgc gctggccata tcttccagct cctgagtcga tgccttctcc agattctcaa
     1741 tcactgccag cgccgggccg ctcatttcag gtttcagctc agcaattagc gccatcagca
     1801 gcttttgcca gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg
     1861 cgctggcttc tttaatgcgc gcggtcagat ccatctccag cggatggtcg tacagcacca
     1921 cttcctgggc gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt
     1981 ctgccagctc gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca
     2041 ataacggcgg aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga
     2101 ttatgcgaat actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg
     2161 cgggtgatgt ttcttcgccc aggcgttggt aacccatcct tccaccatgg cggtaatcgt
     2221 gcctttcacc caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc
     2281 ggcaaatgaa tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa
     2341 ataaggacgc cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc
     2401 ccagaaaacg catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac
     2461 gacgatctta cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttttgatg
     2521 ccagtaacgg aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg
     2581 cagaattcgc gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa
     2641 caaaaagccc agcccgctca ccgccgccag gatgaagcag aaggcggtga tccagtggtt
     2701 gatacgttcc ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg
     2761 tctttctctt cgtgcagatt attctcttcc tcatccgcac ggttcggacc gacacccacg
     2821 tagtggaaga tactggccgc aaaggtagcc gcaaagccaa cagccgcgag cggtttccag
     2881 atgcctttcc agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac
     2941 agatttggct tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc
     3001 ggatcgtaca gacccgcatt gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc
     3061 gccagcgttt tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc
     3121 acgcaggctg gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg
     3181 cggttgtctt ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg
     3241 cagccaatgc actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca
     3301 ccttccgccg ggcacgcttt caggcagcct ggatcggaac agtgcataca gccgtctttg
     3361 cggatcagcc attccagttt gtcgttctgc tccacttccg agaagcgcat taccgtccac
     3421 gatttggcgc ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg
     3481 cgaatgtcgt tccactctga acacgccacc tgacaggctt tacagccgat acaggtggta
     3541 acgtcgatga gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga
     3601 ccgttagtcg cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt
     3661 tacacctttt ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca
     3721 ccaacgaatg gcgttaaagt attggcaata aagccttttt tagctacgcc ttcatagccc
     3781 cagtgaatcg gaataccgat ggtatcgata tctttgccgt tcgctttcag cgtgcgaata
     3841 cgtttggtca ccaccgcttt ggctttgata tagccacggt tggaggagac tttcacggta
     3901 tcgccctggg caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct
     3961 ggctgcaaaa tcgcgttcaa cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga
     4021 cgataggtgg ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct
     4081 ttaaagatac gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc
     4141 ggcgtttcaa acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca
     4201 aacagacgcc ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc
     4261 gctgcgctgt agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac
     4321 tgacgcttcg gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg
     4381 cggcggttaa gcggccatgc ccatgcccag cccagcgtgt taccgaggcc agacggatcg
     4441 gcgttatcac gacgcgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag
     4501 cagccacagg aggttgtacc gtcatcgcgc aattgggcga acgagctaag ttgttggcct
     4561 ttcttgacga taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg
     4621 ctctccatcg ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg
     4681 ttcagcacct gatccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg
     4741 aagataccgg agaggatctc accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc
     4801 cagtgccact gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag
     4861 gtcgatggca gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca
     4921 ccgtggttct gccagaagtt agaggtttca gtgttcagtg ggtcgatggt gacgaggaac
     4981 ttcagtttcg acagacaacc gatcactttg tttttgttcg ggaatgaggc aacagggtta
     5041 aagccctggc agatatagcc attgactttg ccctctttca tcatctcgaa gtattgcagg
     5101 acgtcgtagc ctttatccca cttcggcaac cagtcaaagc cccagctatt ttccgccgtc
     5161 gctttatcac caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag
     5221 tagtttacct ggccttccag cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga
     5281 tcggtctgct tctcgcttgg cagcgtcatg taacctggca gactctgcga cagtagcccc
     5341 aggtccgtca gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccggcc
     5401 atccccatat tgccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca
     5461 acggagtgtt gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg
     5521 gtttctgcga tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt
     5581 tcaaccacat ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc
     5641 ggatgttgca gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag
     5701 gaggatttat cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag
     5761 ccgtaatcct cacgcacgat caggctggcg ttggtatagg tttcggtgta ttcgtggttg
     5821 aatttttcat tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg
     5881 gaacgaatag gggcatagta gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc
     5941 acaatcagct tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa cccgaccggg
     6001 tgagcttcag cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc
     6061 cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg
     6121 tgtcagacac gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc
     6181 gttaaatagc cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag
     6241 cggttaacag tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc
     6301 atcagtttgg cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa
     6361 cctggcgcac ggtattccgg aaacttcaga cggctttcgg agtggatgaa atccaccagg
     6421 ccagcgcctt tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata
     6481 tggaagatag atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca
     6541 cagcctacgg aacaataggt gcaggtatta cgggtttcgc gggtgcgcag cagtttatac
     6601 tgccgggttt ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct
     6661 gccataccgc cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc
     6721 ttgtttcgac attgtcacgt cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag
     6781 ttatttttct ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatct
     6841 aggaagggtt tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag
     6901 aaagtgaaaa aaacacagcg aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt
     6961 caaattgagt tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa
     7021 gaagttcccg ttgcgctggt ctacaacggt atttcgcatg tggtgatgat ggcgtcgccc
     7081 aaagaccttg agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg
     7141 cgcgatatct tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag
     7201 ctttccagcc gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg
     7261 ggctgtggcg tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta
     7321 ccgttcaccc agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat
     7381 tttcagccag tggggcaact gactggttgt actcacgccg ctgcctggat gttgccatct
     7441 ggcgaactgg tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta
     7501 ggccgccggt cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt
     7561 gccagttatg aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg
     7621 tctgccgcga ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac actggtaggt
     7681 ttttgtaaac cgggtcgggc aacggtttat acccatccgc agcgtttgag caattaatta
     7741 aaataagtcc tgcgatatat attaaattgc aggacttatt catttcgtga attttattat
     7801 tttatttata aata
//
LOCUS       DADLSE010000161         7798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLSE010000161 DADLSE010000000
VERSION     DADLSE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.162"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            283..465
                     /locus_tag="JN692_003517"
     CDS             283..465
                     /locus_tag="JN692_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265725.1"
                     /translation="MRCEMLHRYVRRKCSFRRSSASSLTDSLRSVVRLRRAVSAHAKT
                     DIRLSTESGDNTGKNM"
     gene            482..717
                     /locus_tag="JN692_003518"
                     /pseudo
     CDS             482..717
                     /locus_tag="JN692_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229779.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            742..876
                     /locus_tag="JN692_003519"
     CDS             742..876
                     /locus_tag="JN692_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7265726.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(1121..2230)
                     /gene="fepE"
                     /locus_tag="JN692_003520"
     CDS             complement(1121..2230)
                     /gene="fepE"
                     /locus_tag="JN692_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7265727.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFCLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKPSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2650..3018)
                     /locus_tag="JN692_003521"
                     /pseudo
     CDS             complement(2650..3018)
                     /locus_tag="JN692_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3796..4487)
                     /locus_tag="JN692_003522"
                     /pseudo
     CDS             complement(3796..4487)
                     /locus_tag="JN692_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5083..6297
                     /locus_tag="JN692_003523"
     CDS             5083..6297
                     /locus_tag="JN692_003523"
                     /inference="COORDINATES: protein motif:HMM:NF013080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAY7265728.1"
                     /translation="MVVVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIK
                     SKYTKETVTNDSYSHEIINGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNN
                     YYNHTAATFRGLRGQLTSSYQMTGGYHKDNSGLGQASKVEIEHKLNTYNYITKLPIIL
                     EENGYHTYFQASNSIDDPLSLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLN
                     EAKQPFFYGVYTVGTHLGLDSPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSK
                     DTIVIITSDHASYPSEDYKKTFKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLT
                     PTILDILGIKESHNMFIGESLFEKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKT
                     ELIKQLQLFGDR"
     gene            complement(6366..7102)
                     /locus_tag="JN692_003524"
                     /pseudo
     CDS             complement(6366..7102)
                     /locus_tag="JN692_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009652796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replicase"
     gene            7171..7443
                     /locus_tag="JN692_003525"
     CDS             7171..7443
                     /locus_tag="JN692_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265729.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMHPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            7424..>7798
                     /locus_tag="JN692_003526"
     CDS             7424..>7798
                     /locus_tag="JN692_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7265730.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPDCPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLN"
BASE COUNT         2297 a         1758 c         1602 g         2141 t
ORIGIN      
        1 ggacagcggt atcgtctggt ttttcaattt cctatacgtg aactccagaa cagcatccag
       61 attcgctttg aggccgaaag tgaaacagga acataacgaa gctgacggag gcggtcacgg
      121 ctttcccgtg ggcgttttcg tcagcagaca agcccgtcag ggcgcgtcag ggggttttag
      181 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
      241 aattatgcgg cggcagagca gatggtgaga gaacagcgac atatgaggtg tgaaatgctg
      301 cacagatacg taaggagaaa atgcagcttc cggcgctctt ccgcttcctc gctcactgac
      361 tcgctgcgct cggtcgttcg gctgcggcga gcggtgtctg ctcacgcaaa gacggatata
      421 cggttatcca cagaatcagg ggataacacc ggaaaaaaca tgtgagcaaa aaagaggccc
      481 cgaaaaaagg ccgcgccggt ggcgtttttc cataggctcc gcccccctga caagcatcac
      541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gacttaaaga taccaggcgt
      601 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
      661 ttccgctgtt atggccgcgt tcgtctcatt ccacgcctga cactcagttc cgggtaggca
      721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttaacgcc attggcagcg
      841 gccactggta aacaggatgt gcgagagatg tattgagagt tcttgaagtg gcagccttac
      901 tgaggctaca ctagaaggac agatttggtg acatgtctcg cacaagactg ttaccacggt
      961 tcagaagttc cccaacttac tgaaccttcg aaaaaccacc tccccaggtg gttttttcgt
     1021 tttctagagt agaagactac ggctgtaagg gaatgatctc aagaagatcc tttgaacttt
     1081 ttataaaagt tgcttataaa atgatgcatc agcacaactt ttactcaatc acactcatct
     1141 ttctggcctc catggcatga cgtaaaagta tatagccaca tgcaagcatc cctccaatga
     1201 cagaagaaag aattacaatt atggcattac ttggcccatc ctttctgacc ggtaacgttg
     1261 ggctcaattg atactgaaac ggcataaaat caacatcccc cactttcgcc acaaccaatt
     1321 gattcacaac atattgacga tttcgcaact ccccactcaa ttccgaaaca tccttgattg
     1381 attttttgat gttcaattta gttgctatac catcagaacc gagagccaca ggaaaatctg
     1441 ggtcatcctt cataatctga ccattgctgt atacaggttt ttttattcca gccgcattag
     1501 caacctccag tgaataattg aggcgtcgaa tatctgcatt cagctggttt ctaattttta
     1561 tctcatccag tgcaagaatt tctttttcaa cattagtttt aacttctagt ttatttctta
     1621 tatcttccat caaatccctt acaacaaggg aagaaacata attaatatac ccagacaaca
     1681 catcgtgagc ttctttactt gttggagcag taaaactcag agtccatgat acatacaatg
     1741 atggcttgtc gtttttctta ctggcattat catcaatcga cttcatattt ttactcaact
     1801 caacaattgc acgatgcagt tcgagagcgc taatgttatt ctttttaata atctccataa
     1861 cataaggaga tgatctcata tactcattca acgaattaac ggactgaaat ttcttaataa
     1921 acagagcaaa tgcgctatca cgattgatat caaaatccac tcccagaaca tgaagcttgg
     1981 aaagctcctt atttaattca ctccattgaa cagactcagc aggagtaacc acagcagaac
     2041 tagtccattt ttgtggcaaa acaaaagaaa caagaaagcc aatacaagca aaaaatagaa
     2101 tcaccccaac aatttttatt tttgctgccc ataaaacgct aatcaagcaa aaaaggtcaa
     2161 tttcttcatt accattcggc aacatgttgt aattagaaaa atgttcgtca gagacctttg
     2221 agattttcat tgaaaaaaac acaatatatt aacatatggt ttttttattc tatcatatgt
     2281 taggaaaaaa cctaaaaacc attcagcaga catttaacat aacattttat tgcgtgcacc
     2341 gcctcagagg ctctcagggc atttctgacg ggaaaccggc aaaacaggca acaaaccacc
     2401 cgaaaacccg ccagaaacgc gcaggctctg tttttactgc atgcatgagt atgcacgcaa
     2461 gtgcatagcg cgaaaattca ccccattcag ccagggtaag tcctgtttcc ggcattcttc
     2521 ccatccgaaa acggtgaact gggacgcaat ttattgcgtg gccgtgaagc cgtaaggcac
     2581 ggtaggtaat tgcaggtatc cgacaatttc gagtagagga tccactattt cactacttag
     2641 aattcaaaat cagaatcctt tcttcctgct aaggctatct ctgaatgact caaccactct
     2701 ttgagattct tcttctgctc acatgaagtc attcccggac agaaaaaaac cattgtcagg
     2761 cccactggct tcagagaaat tcgtttctgc agcagacaca gaatccacat cagcaagaga
     2821 cggttcatct gtactttttc tcaatctgta aagagcctta tcaaacgcaa ccagtgagat
     2881 attcaggccg taacgttcat tcagcacagt cacaatctct tccctacgga taccattttt
     2941 cttccaggtt tctatatcgt tcttggaatc cctgacagcc gcaatggctg ttcttggacc
     3001 agggttaaaa ttcgccatat accgcccata atgtcttaaa tttgtctcat aatagtccaa
     3061 aaataagatt gaaacgcaaa aaatgtctta caattatctt attgttgtcc tttccggttc
     3121 atgtatccat gaaccgcctg atgtggaacg ctttttatcg atctcatgcg cagccaaaga
     3181 tagattcatt ctttaaaagt caggttacat tatcccttaa atggtcatac accatacgca
     3241 accgcgattt tcatcgcaat tgcatgaatt aaaaggtaaa aactgaagtc cccgtttatt
     3301 tatgtaatac gcaacgcatt acaaccacct gtaattacat gaaaataaag aaataagata
     3361 taataccggc gtattaccac gtacgcgggt aagcgaaaaa gctataacgc ttcccggccg
     3421 acaggcggcc caaaatccac cggggcgtgc aggttctctt cagtaacctg caccattaaa
     3481 tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg aatccagttg ttcatcatgt
     3541 tcagatggca tggggtgata tccagtagct gagaggtggt tagcgggttt cgggacgcag
     3601 ccctaaacca gtcacgtagc gctagcggag tgtatactga cttaggcggt agtgtagtat
     3661 gacagattct gtggttacgg caaagtaaca gccctgtcta ggagagggct gttatctgat
     3721 ataggcttgt ttttttctca ccgtttttgc gagcgctttt tcctgccaag aggtaggtag
     3781 ggtaatgaat ccaacttact gatagtgttt tatgtttaga taatgcccga tgactttgtc
     3841 atgcagctcc accgattttg agaacgacag tgacttccgt cccggccttg ccagatgctg
     3901 cctcagatta tgccgctcaa ttcgctgcgt atatcgcttg ctgataacgt gcagcttttc
     3961 cttcaggcgg gattcataaa gcggccatcc gtccgtcatc aataccacca cgtcaaaggc
     4021 cgatagcagg ctcagaagac gctccagcgt gaatattgtg cgttcaccaa aaacgtgggc
     4081 cacgacagtc ctgcgtaccc tgtcatacgc gtaaaacagc cagcgctggc gtgatttagc
     4141 cccgacgtag ctccactgtt cgtccatttc cgcgcagacg atgacgttcc tgcccggctg
     4201 tatgcgcgag gttaccgact gcggcctgag ttttttaaat ggcggaaaat cgtgttgagg
     4261 ccaacgccaa taaagcgggc ggttgcccgg catccgacgc cattcatggc catatcaatg
     4321 attttctggt gtgtaccggg ttgagaggcg gtgtaagtga actgtagctg ccatgtttta
     4381 cggcagtgag agcagagata gcgctgatgt ccggcagtac ttttaccgtt acgcacaacg
     4441 ccgtcagtag cggaacagga gggacagctg atggaaatgg aagccaccgg agcacctcaa
     4501 aagcaccgtc atccactaaa tcagtaagtt ggaagcatca ccgcactagc agacagttaa
     4561 ttaaaaaagt ataatccctc tccggattta ttgaataatg gagaattatg ctgttttcta
     4621 tgtcttattt agtcaaatgc gcttatcaat attgtgataa gctcccgagt ctaaacttaa
     4681 cttactgatt ttaggttgag gaagaccggc atttttgtag atccacggca taatatctat
     4741 ttattcaaca acgcccccca aaaaaaggtt tttataaatg tttttaattt caatcattca
     4801 aatgttctcg gttatgatta taatgcttaa tataaaaata aaagatgcat ttagagcaat
     4861 tattctgctt ttacttctat tactatccat ggttatatct attgaaataa gcagcattat
     4921 atcaataggt gagtacatac ccacacttgc actagcaaat atatcagaga caaatagtgt
     4981 tggctggaaa ataaaactac taccttttct tgtgttcttg tcatttatga ttgcatcatt
     5041 gattttgaaa agattgataa aagaaaaaaa ataagaaata taatggttgt tgttttttta
     5101 ttatcattgt taataccaat atctccaata aatgcttttt tttcttcatc atatgaagtt
     5161 tattcggaag caaaagaata caatatgtca ccacatgaaa taagtgatat aaagtcaaaa
     5221 tacacaaaag aaacagtaac aaacgacagt tactcacatg aaataataaa tggaaacgga
     5281 aaaaatattt tagtaatatt tattgaaggt atgtcttcat tggtaatatc taaggagcta
     5341 accccgaatc tatatgaatt tagaaaaaaa tcaatatcat ttaacaacta ttacaaccat
     5401 accgcagcta catttagagg tttaagagga cagttaacct catcatatca aatgaccggt
     5461 gggtaccata aagataattc tggccttggc caagcatcta aagtagagat cgaacataaa
     5521 ctaaatactt acaattacat aacaaaatta ccaatcatac ttgaggaaaa tggttaccat
     5581 acatattttc aagcatctaa ttcaatagat gacccattga gtttaatgct atcaacttta
     5641 aagtttaatc atatatacgg aagggaggac tataacaaaa agtcaggtga actcacagac
     5701 aaagagtctt tcgatttatt atttaaaaaa ttaaatgagg ctaaacaacc tttcttctat
     5761 ggtgtttata ctgtaggtac acaccttggc ttagatagcc ctgatgtaaa gttttatgat
     5821 gggagaaatg aatatttaaa tcactttcat caattagacc attggtttgg tgattttata
     5881 tctcatttta ataagacaag cttatcgaaa gatacaattg tcataatcac aagtgaccat
     5941 gcaagttacc catcagaaga ttacaaaaaa acattcaaaa ctgataatac tttttttgtt
     6001 gatcagattc cattaataat atggagtaat ggtgttgagt tcaaagacat taatgcacac
     6061 ggtttaaatt cactatcttt aacgccaaca atactagata ttcttgggat caaggagtca
     6121 cacaacatgt tcataggtga gtctctattt gaaaaagaaa aaaataaaaa tgaactcaac
     6181 tgtacatctg caataggtga cctgtatgtg aaaacgtgta atggaaaaat ggaaatcgag
     6241 aaaagaaaag aaaaaacaga attaataaaa caattgcaac tttttggtga tagataatta
     6301 aaaggcggtt ttaccccatg ttgattatta gtccacgtgg ggttagttta aaatgataat
     6361 aaaacttact ttttttcatt cttgagccgc ctgtagaatg tggctcgact aacacctaaa
     6421 gactcccatt ctttttttaa agttatatga attaatatta aaactacctt taggccgtcc
     6481 tcctgaattg cttagctttc ctcctttacc ccctctcttt ctttgaattt ctgaacggtg
     6541 cgtatttttt acatactcaa taaaacgatc ctctgaaaaa ttattcgctg tccattttga
     6601 gatgcttttt gatattccaa gaacttcatt ttcacttaat ggtgatacga attgtaaatt
     6661 gtaggcacag gctcgttcat agcaggcttg taaccactgc tcatactcag gccacccctg
     6721 acggatggca cgatatgccc acttgcgggt tttgtcgaac agagtgcagt tacggcctaa
     6781 accataatcg gcggcaattt tccagtgact gtggttcgga ttcttgcaaa ttagccccga
     6841 atatcccgtg tcagctccca attttctacg tagcgcattc tctacgcgca gctgcgtagc
     6901 gtaacggttt catttttccg tcaggtgctg tgcgtataga ggtttccagc gcataaagca
     6961 ggtgcgcgtg tccgttttcg gggttggtga tggtcagcgt aggtgcggga gcgttgcggt
     7021 cgctccagtc aatggctgcg ccgatgcggt caacatcaaa tcccagccag aacatggcgt
     7081 gaggctgatt gaaccacggc agcctggcgg ctgcaaatcg gggtgtggca gaatatgagc
     7141 tttcagagta accatccaga atgagttccc atgtcacaca acctggagca ccagaaagtc
     7201 cacaccagaa tggtgaaaga ggttctgaaa gccgtcgcca gagccaataa tcacccttat
     7261 aagtccgtat ttgcagactt tatcaccgga catccctcct gcacggtctg tttctgggaa
     7321 accttccaca aaatgcaccc ggattcaccg tacgaatacg tcacgttctg ccatacctgc
     7381 cgtcgttttg atttatacga aacagaagcg gagatgaagg cggatgaccc gaagtggtgg
     7441 tgattttcag ccccgaccgc tgaaacgcct gttcacagcc aaccagtgct ggacatcctt
     7501 cctggatgcg ggcggtctgc gcgatatcga ggttgaggct gtcacaaaaa tgctggcctg
     7561 cggcacgcgg atactgggcg tgaaggaata caactgcgat aaacctgact gcccccacgt
     7621 cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac
     7681 agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt
     7741 cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaat
//
LOCUS       DADLSE010000162         7785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLSE010000162 DADLSE010000000
VERSION     DADLSE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.163"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            111..644
                     /gene="ymdB"
                     /locus_tag="JN692_003527"
     CDS             111..644
                     /gene="ymdB"
                     /locus_tag="JN692_003527"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7265731.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWCGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            586..2067
                     /locus_tag="JN692_003528"
     CDS             586..2067
                     /locus_tag="JN692_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7265732.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN692_003529"
     CDS             complement(2075..3232)
                     /gene="mdoC"
                     /locus_tag="JN692_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7265733.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPRLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIDYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3626..5161
                     /gene="mdoG"
                     /locus_tag="JN692_003530"
     CDS             3626..5161
                     /gene="mdoG"
                     /locus_tag="JN692_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7265734.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5154..7697
                     /gene="mdoH"
                     /locus_tag="JN692_003531"
     CDS             5154..7697
                     /gene="mdoH"
                     /locus_tag="JN692_003531"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7265735.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPLVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         1927 a         1857 c         2056 g         1945 t
ORIGIN      
        1 catcgaatat aactaagttt ctgaacaact cacgttgctg agcagaaaaa tgcgatttaa
       61 ccaaaaagcc tgctgtacac ttaagaaaca agaaggtgta aggaggcgtt atgaaaacgc
      121 gtattcatgt tgtgcagggt gatattacca aactggccgt cgatgtgatt gtgaatgcgg
      181 ctaatccgtc attaatggga ggcggcggcg tcgatggggc cattcatcgc gcagcgggtc
      241 cggccctgct ggatgcctgt ttaaaagtca ggcaacagca gggcgattgc cctacggggc
      301 atgccgttat tacgcttgca ggcgatcttc ccgctaaagc cgtagtacac accgtcgggc
      361 cagtctggtg tggtggtgaa caaaacgaag accagctttt gcaggatgcc tatctgaata
      421 gcctacgact ggtggcggca aacagctata cgtcagtggc ttttcctgca atcagtactg
      481 gggtttatgg ttaccctcgt gcggcagcgg ctgaaatcgc agttaaaacc gtttcagaat
      541 ttattacccg tcacgcttta cccgaacagg tatactttgt ctgttatgat gaagaaaacg
      601 cccacctcta cgaaagactc cttacccaac aaggagatga atgatttgcc ccggctggcg
      661 agcgcggtgc tgccactgtg ttcgcaacat cccggtcagt gtggtctttt ccctctggag
      721 aaaagtctgg atgcgtttgc cgcccggtat cgtctggccg aaatggcaga gcatacgctg
      781 gatgttcagt attacatctg gcaggacgat atgtcggggc ggttactgtt ttccgccctg
      841 ttagccgcag caaagcgtgg cgttcgcgtc cgtttgttgc tggacgacaa caatacgccc
      901 ggacttgacg acattttacg cttgcttgac agtcatccac gcattgaagt ccggcttttt
      961 aatcctttct cgtttcgctt gctacgtccg cttggatata tcaccgactt ttcccgtctc
     1021 aatcgccgga tgcacaataa aagtttcact gtcgatggcg tggttaccct agtgggagga
     1081 cgaaatattg gtgatgccta ttttggtgca ggggaagagc cacttttttc ggatttagat
     1141 gtcatggcaa taggccccgt ggtagaggac gttgccgatg atttcgcccg ctactggtat
     1201 tgcaaatcgg tttcaccctt acagcaggtg ctggatgtcc cggaaggtga aatggcggat
     1261 cgcatcgagt tacccgcctc ctggcataac gatgccatga cgcatcgtta tttacgcaaa
     1321 atggaatcca gtccatttat aaatcatctg gttgatggaa cgttgccgct tatctgggcg
     1381 aagacacgtt tattaagtga tgatccggcg aaaggggagg gcaaggcaaa acggcattca
     1441 ctgttaccgc agcgtctgtt cgatatcatg ggctcaccca gtgaacgcat cgatattatc
     1501 tcttcctatt ttgtaccgac acgcgcaggt gtggcgcaac tcttacggat ggtgagaaaa
     1561 ggggtaaaga ttgcgatcct aaccaattct cttgccgcta acgatgttgc tgtcgtccat
     1621 gccggatacg cgcgctggcg caaaaaattg ctccgctatg gcgtggaatt atatgaactc
     1681 aagccgacgc gtgaacaaag cagtacgtta cacgatcgcg gcataaccgg taattccggt
     1741 gccagcctgc atgctaaaac ttttagcatc gatggtaaaa cggtgtttat cggctctttc
     1801 aatttcgatc cgcgttcaac attgctcaat actgaaatgg gcttcgtgat agagagcgaa
     1861 acgctggcac agttaattga taaacgcttt attcagagcc agtacgatgc ggcctggcag
     1921 ctccgtctgg acaggtgggg acggatcaac tgggttgatc gtcatgcaaa gaaagaggtt
     1981 attctcaaaa aagaacccgc caccagtttc tggaagcggg ttatggtcag actggcgtcg
     2041 atattgcccg tggaatggtt attgtaaatg tggcttaacg ggctggtgct ttatcgttct
     2101 cacgcttaac aaccggttta ccagagaaca aaaacttcag taacgggatg cgtaaatgaa
     2161 tttcatacag aattatcgca atccctacta cgaatatcag gccacagaga aaaccaagcc
     2221 agttggaggt gatgtgcggt gtaatgtatg cgccgaaaaa cagcgttaac gggtggtgaa
     2281 ccagatagat aaacagcgat gcgttaacaa aataagtcac ccgcgctgac tggaagttaa
     2341 gcaaacggtg gccaaaggag aagaccacat tcaccatcca caggccgagg accatggtga
     2401 tcaccgactc ggtttcgtac atccaggcat cgccactgcc atagcgctgg ttgagtaaat
     2461 aagcgacaaa cgccaatgct gcagcgaggg tacagccacg agacggcgtg gtaaacaagg
     2521 ctttaagatg agggaaaatg aaagccagtg cgccgaggat aaagaacggt aaataaaata
     2581 gcgtttgcat gacaataaaa ttgaacatgc cattactcag aatgggtgga tacacaataa
     2641 aaatcgttct tcttattacc gcatagtcga tgccgaggca taaaaaaatc accgatagtt
     2701 ttaccatcga gaatttttta ttcgttttat cagaattttc taaattattt ctgatgcgct
     2761 taaatatcca tacacacagc gtcgtcatga ctactaacac cagtaaaaac cacaggtgtg
     2821 atattaattc ccaggccaac gtattatatt tgtcatacaa tgatagccta ggccaacttt
     2881 ccgctttccc tttgacatat tgcagcataa taaattgcgg taatgtcagt agggggatgg
     2941 ctgttaacat cgggatacct acacgttcga cacgtacttt ccaccatttt ttcaagggat
     3001 agcgtaaaaa aagcatgtag gaaaagtatc cggatataac gaaaaatacc tgcatgcgga
     3061 acgagtggat gaagtcatta aaaagggtca gccacaatga cggttcggcg ctattcacat
     3121 gccatgtatg gctcgaatag attaaagaaa tatgaaaagg gatccctaac aacatcagcc
     3181 aggcgcggat ggagtcgagg aaatattcac gttgcgcggg tactggattc atatatggtt
     3241 aactaatctc ggatttttcg tcttatccct gtcgggttat gcctttaggc ttgttgccat
     3301 agcgacaccg acctgaccgc gccaggcgca ggcttcaagg tttttatgca tagcatcatc
     3361 gctaccacta accagaatgg aagcgtctgt aagacggttg ataaataaat ttgctggcaa
     3421 accctacacg aagtcgatgc ttctgtcttt aggagaagca cggaaagtga aaacggttgc
     3481 aatcaggtgc ttaatccatg agccagcgtg ctgaacgata ccgggattca gttgtcggaa
     3541 tggctggtta tccattaaaa tagatcggat cgatataagc acacaaaggg ggaagtgctt
     3601 actaattatg aaacataaac tacaaatgat gaaaatgcgt tggttgagtg ctgcagtaat
     3661 gttaaccctg tatacatctt caagctgggc tttcagtatt gatgatgtcg caaagcaagc
     3721 tcaatcctta gccgggaaag gctatgaggc gcctaaaagc aacttgccct ccgttttccg
     3781 cgacatgaaa tacgcggact atcagcagat ccagtttaat catgacaaag cgtactggaa
     3841 caatctgaag accccattca aactcgagtt ctaccatcag ggtatgtact tcgatacccc
     3901 ggtcaaaata aatgaagtga ctgccaccgc agtcaaacga atcaaataca gcccggatta
     3961 tttcactttc ggcgatgttc agcatgacaa agacacggta aaagaccttg gttttgccgg
     4021 ttttaaagtg ctttacccga tcaacagcaa agataaaaac gatgaaatcg tcagcatgct
     4081 cggggccagc tatttccgcg tgattggtgc aggtcaggtt tatggccttt ctgcccgcgg
     4141 cctagcaatt gataccgctt tgccatcggg tgaagaattt ccacgcttca aagagttctg
     4201 gatcgagcgt ccaaaaccga ctgataaacg tttaaccatt tatgcattgc ttgactcgcc
     4261 gcgtgcgaca ggtgcttaca aattcgtggt tatgccaggg cgtgacacgg ttgtggatgt
     4321 gcagtcgaaa atctatctgc gcgataaagt cggcaaactg ggggttgcac cgttaaccag
     4381 tatgttcctg tttgggccga accaaccgtc tcctgcaaat aactatcgtc cggagttgca
     4441 cgactctaac ggtctgtcta tccatgctgg taatggcgaa tggatctggc gtccgttgaa
     4501 taacccgaaa catttagcgg tcagcagctt ctccatggaa aacccgcaag gctttggtct
     4561 gttgcagcga ggtcgtgatt tctcccgctt tgaagatctc gatgatcgtt acgatctccg
     4621 tccaagcgca tgggtgactc cgaaagggga gtggggcaaa ggcagcgttg agctggtgga
     4681 aattccaacc aacgatgaaa ccaacgataa catcgtcgct tactggacgc cggatcagct
     4741 gccggagccg ggtaaagaga tgaactttaa atacaccatc accttcagcc gtgatgaaga
     4801 caaactgcat gcgccagata acgcatgggt gcaacaaacg cgtcgttcaa cgggggatgt
     4861 gaagcagtcg aacctgattc gccagcctga cggtactatc gcctttgtgg tcgattttac
     4921 cggtgcagag atgaaaaaac tgccagagga taccccggtc acagcgcaaa ccagcattgg
     4981 tgataatggt gagatagttg aaagcacggt gcgctataac ccggttacca aaggctggcg
     5041 tctggtgatg cgtgtgaaag tgaaagatgc caagaaaacc actgaaatgc gtgctgcgct
     5101 ggtgaatgcc gatcagacgt tgagtgaaac ctggagctac cagttacctg ccaatgaata
     5161 agacaactga gtacattgac gcaatgccca tcgccgcaag cgagaaagca gcattgccga
     5221 agactgatat ccgcgccgtt catcaggcgc tggatgccga acaccgcacc tgggcgcggg
     5281 aggatgattc cccgcaaggc tcggtaaagg cgcgtctgga acaagcctgg ccagattcac
     5341 ttgctgatgg acagttaatt aaagacgacg aagggcgcga tcagcttaag gcgatgccag
     5401 aagcaaaacg ctcctcgatg tttcccgacc cgtggcgtac caacccggta ggccgtttct
     5461 gggatcgcct gcgtggacgc gatgtcacgc cgcgctatct ggctcgtttg accaaagaag
     5521 agcaggagag cgagcaaaag tggcgtaccg ttggtaccat ccgccgttac attctgttga
     5581 tcctgacgct cgcgcaaact gttgtcgcga cctggtatat gaagaccatt cttccttatc
     5641 agggttgggc gctgattaat cctatggata tggttggtca ggatttgtgg gtttccttta
     5701 tgcagcttct gccttatatg ctgcaaaccg gtatcctgat cctttttgcg gtactgttct
     5761 gttgggtgtc cgccggattc tggacggcgt tgatgggctt cctgcaactg cttattggtc
     5821 gcgataaata cagtatatct gcgtcaacag ttggcgatga accattaaac ccggagcatc
     5881 gcacggcgtt gatcatgcct atctgtaacg aagacgtgaa ccgtgttttt gctggcttgc
     5941 gtgcaacgtg ggaatcagta aaagccaccg ggaatgccaa acactttgat gtctacattc
     6001 tgagtgacag ttataacccg gatatctgcg tcgcagagca aaaagcctgg atggagctta
     6061 tcgctgaagt cggtggcgaa ggtcagattt tctatcgccg ccgccgtcgc cgcgtgaagc
     6121 gtaaaagcgg taatatcgat gacttctgcc gtcgctgggg cagccagtac agctacatgg
     6181 tggtgctgga tgctgactcg gtaatgaccg gtgattgttt atgcggcctg gtgcgcttga
     6241 tggaagccaa cccgaacgcc gggatcattc agtcgtcgcc gaaagcgtcc ggtatggata
     6301 cgctgtatgc gcgctgtcag cagttcgcga cccgcgtgta tgggccactg tttacagccg
     6361 gtttgcactt ctggcaactt ggcgagtcgc actactgggg acataacgcg attatccgcg
     6421 tgaaaccatt tatcgagcac tgcgcactgg ctccgctgcc gggcgaaggt tcctttgccg
     6481 gttcaatcct gtcacatgac ttcgtagaag cggcgttgat gcgccgtgca ggttgggggg
     6541 tctggattgc ttacgatctc ccgggttctt atgaagaatt gccgcctaac ttgcttgatg
     6601 agctaaaacg tgaccgccgc tggtgccacg gtaacctgat gaacttccgt ctgttcctgg
     6661 ttaagggtat gcacccggtt caccgtgcgg tgttcctgac gggcgtgatg tcttatctct
     6721 ccgctccgct gtggtttatg ttcctcgcgc tctctactgc attgcaggta gtgcatgcgt
     6781 tgaccgaacc gcaatacttc ctgcaaccac ggcagttgtt cccggtgtgg ccgcagtggc
     6841 gtcctgagct ggcgattgca ctttttgctt cgaccatggt gctgttgttc ctgccgaagt
     6901 tattgagcat tttgcttatc tggtgcaaag gaacgaaaga atacggcggc ttctggcgcg
     6961 ttacattatc gttgctgctg gaagtgctgt tttccgtgct gctggctccg gtacgcatgc
     7021 tgttccatac ggtcttcgtt gtcagcgcgt tccttggctg ggaagtggtg tggaattcac
     7081 cgcagcgtga tgatgactcc acttcatggg gcgaagcgtt caaacgccac ggctcacagc
     7141 tgctgttggg gttagtgtgg gctgttggga tggcgtggct ggatctgcgt ttcctgttct
     7201 ggctggcacc gattgtcttc tcgttgatcc tgtcaccgtt tgtttcggtg atttccagcc
     7261 gtgccaccgt tggtctgcgc accaaacgct ggaaactgtt cctgatcccg gaagagtatt
     7321 caccgccgct ggtgctggtt gataccgatc ggttccttga gatgaatcgc caacgctccc
     7381 ttgacgatgg ctttatgcac gcagtgttta acccgtcatt taacgctctg gcaaccgcaa
     7441 tggcgacagc gcgtcaccgc gccagtaagg tgctggaaat cgcccgtgac cgccacgttg
     7501 aacaggcgct taacgagacg ccagagaagc tgaatcgcga tcgtcgcctg gtgctgctaa
     7561 gcgatccggt gacgatggcc cgtctgcatt tccgcgtctg gaattcccct gaaagatatt
     7621 cttcatgggt gagttattac gaagggataa agctcaatcc actggcattg cgtaaaccgg
     7681 atgcggcttc gcaataaaaa cgtagttgcc tgatgcgcta cgcttatcag gcctacatcg
     7741 ttcctgcaat ttattgattt tgcaagattt tgtaggtcgg ataag
//
LOCUS       DADLSE010000163         7774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLSE010000163 DADLSE010000000
VERSION     DADLSE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.164"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(108..1361)
                     /locus_tag="JN692_003532"
     CDS             complement(108..1361)
                     /locus_tag="JN692_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265736.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFC
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTHYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1468..2388
                     /locus_tag="JN692_003533"
     CDS             1468..2388
                     /locus_tag="JN692_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7265737.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNCSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            2523..3743
                     /gene="mlc"
                     /locus_tag="JN692_003534"
                     /gene_synonym="dgsA"
     CDS             2523..3743
                     /gene="mlc"
                     /locus_tag="JN692_003534"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7265738.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3868..4563
                     /gene="bioD"
                     /locus_tag="JN692_003535"
     CDS             3868..4563
                     /gene="bioD"
                     /locus_tag="JN692_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7265739.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KDTPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN692_003536"
     CDS             complement(4516..5808)
                     /gene="clcB"
                     /locus_tag="JN692_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7265740.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN692_003537"
     CDS             complement(5967..6581)
                     /gene="dmsD"
                     /locus_tag="JN692_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7265741.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6624..7478)
                     /locus_tag="JN692_003538"
     CDS             complement(6624..7478)
                     /locus_tag="JN692_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7265742.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7480..>7774)
                     /locus_tag="JN692_003539"
     CDS             complement(7480..>7774)
                     /locus_tag="JN692_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265743.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
BASE COUNT         2048 a         1988 c         2043 g         1695 t
ORIGIN      
        1 cgctgcggga attggctggc gcttcgtcta ttttaggatg gctattaacg ttggtttgag
       61 caggcaataa gtccggacgg gtatttaccg cagtccggac ttatttttca ggcgtgcaga
      121 cgacgatgca aacgcgtccc gaccagcaga gcaatgacca gcatcagagc aataaatgcg
      181 ccgacgccgt tccagccata gttatgccag aaaacaccac ccagcgtccc ggcaatactc
      241 gaccccagat agtaactgaa cagatacagc gaggaggcct ggcctttagc gcgttttgca
      301 cgggggccga tccagctgct ggctactgag tgggctgcga agaatccggc tgagaagagt
      361 aacattccgg caaagatcag ccacagcgag ctaaataagg tcatcagtaa accaaacagc
      421 ataactcccg tcgaaaacaa catcaccgga ccgcgcccat agtgggtggt catggttccg
      481 gctttgggtg agctccatgt accggtcaaa taagccagcg ataataagcc aaccacggcc
      541 tgactgacat gccaggggga aagcatcaac cgatagccga tgtaattaaa cagcgtgacg
      601 aacgacccca tcagcaaaaa gccttctgcg aacaataacg gtaatccccg gtcacgccag
      661 tgcagacgaa agttgataaa taacgtctta gggcgcagcg aagttgggca aaaatggcgt
      721 gattcaggga ggattttcca gaacatcaac gccgaggcca gcgcgaaaca accgattgcc
      781 gccagagcaa ttcgccagtt gaaaaagtcc gtgaagacac cactaattaa gcgtccgctc
      841 atgccgccaa ttgagttgcc gctgatatac aaccccatcg aaaaggccac gaaactgggg
      901 tggatttcct cgctaagata agtcatgcca acagctgcca cgccacttaa cgaaagccca
      961 atcaaggcgc gcataatcaa aatgccgtgc cagctggtca tcattgtcga aagtaacgta
     1021 caaatggagg ccaacagtag cgccgtgacc atcactggtt tgcgaccaat ggcatcggat
     1081 agcgggccag taaatagcaa accgatagcc aacatcgccg tggaaatgga cagtgaaata
     1141 ctactgttcg cgggcgttaa gccaaactcc tgcgaaagca ccggaaggat aggctgcaca
     1201 caatagagaa gtgcaaaggt tgccagtccg gcagagaaca gcgccagggt gacgcgcata
     1261 aattgcggcg taccgcgttt aataaattga tttggctgag aaatgctttg cttgtcagtg
     1321 tcgcttgccg gagcgccatc aacagttgta gtacggctca cttgaaatcc ttgctaaata
     1381 tgcctgtgga tcaggcttat acatagggta ggaaaatcga attgttctgt ctaatatatt
     1441 aataatctca aataagatgt tttaaatatg aatattgaac ttcgtcatct gcgttacttt
     1501 gttgctgttg cggaagagct gcattttggg cgcgcggctg cccgcctgaa tatttcgcaa
     1561 ccgccgctaa gtcagcagat tcaggcactg gagcaacaaa tcggtgcccg actgctggca
     1621 cgaactaatt gcagtgtatt gctgacggca gcaggaaaac agtttcttgc agatagtcgg
     1681 caaatcctgt ctatggtgga tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa
     1741 gcgggggagt tgcgtattgg ttttacttcg tccgctcctt ttattcgggc ggtgtccgat
     1801 acgttatcgc tgtttcgccg tgattatcct gatgtccatt tacaaacccg cgaaatgaac
     1861 actcgcgagc aaatcgctcc gctcattgaa ggaacgctgg atatgggatt gctgcgtaac
     1921 acagcgttac cggagacgct tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg
     1981 atcccgcacg atcatcccct ggcaaataat ccgagtgtaa cactggctga actggctgaa
     2041 ctggctgaac tggctgaact ggcgaaagaa ccctttgtct tttttgatcc gcacgtcggg
     2101 actgggctgt atgacgatat tctcggactg atgcgacgtt accatttgac gcccgtcatc
     2161 actcaggagg tgggcgaggc aatgaccatc atcggtctgg tttccgccgg tctgggtgtt
     2221 tcaattttgc ctgcgtcatt taaacgtgtt cagctcaacg aaatgcgctg ggtgccgatt
     2281 gctgaagagg atgcggtttc tgaaatgtgg ttggtctggc cgaaacatca tgaacaaagt
     2341 ccagctgcgc gtaactttcg tattcatctg ctgaatgctc tcaggtgagg gaaatttcag
     2401 cgaaaaagcc cgaaaaatgt gctgttaatc acatgcctaa gtaaaaattt gacgacacgt
     2461 attgaagtgc ttcaccatag cctacagatt atttcggagc gcgaaaatat agggagtatg
     2521 cggtggttgc tgaaaaccag cctgggcaca ttgatcaaat aaagcagacc aacgcgggcg
     2581 cggtttatcg cctgattgat cagcttggtc cagtctcgcg tatcgatctt tcccgtctgg
     2641 cgcagctggc tcctgccagt atcactaaaa ttgtccgtga gatgctcgaa gcacacctgg
     2701 tgcgagagct ggaaatcaaa gaagcgggga accgtggccg tccggcggtg gggctggtgg
     2761 ttgaaactga agcctggcac tatctttctc tgcgcattag tcgcggggag attttccttg
     2821 ctctgcgcga tctgagcagc aaactggtgg tggaagagtc gcaggaactg gcgttaaaag
     2881 atgacttgcc attgctggat cgtatcattt cccatatcga tcagtttttt atccgccacc
     2941 agaaaaaact tgagcgtcta acttcgattg ccataaccct gccgggaatt attgatacgg
     3001 aaaatggtat tgtacatcgc atgccgttct acgaggatgt aaaagagatg ccgctcggcg
     3061 aggcgctgga gcagcatacc ggcgtaccgg tttatattca gcatgatatc agcgcatgga
     3121 cgatggcaga ggccttgttt ggtgcctcac gcggggcgcg cgatgtgatt caggtggtta
     3181 tcgatcacaa cgtgggggtg ggcgtcatta ccgatggtca tctgctacac gcaggcagca
     3241 gtagtctcgt ggaaataggc cacacacagg tcgacccata tggtaaacgc tgttattgcg
     3301 ggaaccacgg ctgcctcgaa accatcgcca gtgtggacag tattcttgag ctggcacagc
     3361 tgcgtctcaa tcaatccatg agctcgatgt tacatgggca accgttaacc gtggactcat
     3421 tgtgtcaggc ggcattgcgc ggcgatctac tggcaaaaga catcattatc ggggtgggcg
     3481 cgcatgtagg gcgcattctt gccatcatgg tgaatttatt taacccacaa aaaatactga
     3541 ttggctcacc gttaagtaaa gcggcagata tcctcttccc ggtcatctca gacagcatcc
     3601 gtcagcaggc ccttcctgcg tatagtcagc acattagcgt tgagagtact cagttttcta
     3661 accagggcac gatggcaggc gctgcactgg taaaagacgc gatgtataac ggttctttgt
     3721 tgattcgtct gttgcagggt taacattttt taactgttct accaaaattt gcgctatctc
     3781 aatttgggcc aggaaagcat aacttagact ttcaaggtta attattttcc tagtttatat
     3841 ttgtgaagca taacggtgga gttagtgatg ctgaagcgtt tctttattac cggtacagac
     3901 acttctgtag ggaaaacggt ggtttcccgc gcattgctac aagcgttagc ctcccaggga
     3961 aaaacggttg cgggatataa acccgtagcg aaggggagca aagatacacc cgaagggctg
     4021 cgtaataaag atgccttggt gttgcagagt gtttcaacca tcgaactgcc ttatgaagca
     4081 gttaatccta tcgcgttaag cgaagaagaa agtagcgtgg cgcacagttg cccaatcaat
     4141 tacaccctca tttcaaacgg cctggcaaac ctgaccgata aagtcgatca tgtcgtggta
     4201 gaagggactg gcggctggcg cagtctgatg aatgatttgc gtccactctc tgaatgggta
     4261 gtgcaggaac aactgccggt gttgatggtt gtcggtattc aggaaggttg cattaaccat
     4321 gcactgctaa cagctcaggc gatcgccaac gacgggctgc cgctcattgg ctgggtggct
     4381 aaccgaatca acccaggact ggcgcattat gcggaaatca ttgatgtgct gggtaaaaaa
     4441 cttccggcac cgctcattgg tgaactgcct tatctgccgc gcgccgaaca gcgtgaactg
     4501 gggcaataca tccgcttagc tatgctgcgc agtgtgctgg cggtagatag agtcacggtg
     4561 taacgtccgc gaaattaccg acgcaattac gcaggcaatc aataaaccgg ggagtagctg
     4621 atactccccg gtcatttcac atatcatcaa cgtcgacata atcggcgcgt gcgtggttgc
     4681 cgccaacagt gtcgccatcc cggtcaatcc gagtaaaagc gtaatttctt cgccatcagg
     4741 gaaccataat cccaggctac gaccatacaa catgccaatg gcaagaccga taaatagcgt
     4801 cggtgtaaag acaccaccgg gtgcgccgga accgctactc gccagcacgg cacacagttt
     4861 acaaaggaag atcccggcaa tgatcattaa cagtggtggg gcggttaaaa aggattgtac
     4921 ggtgctatag ccgttgcccc acactgcagg tgtgaacagg gaaagcagac ccacgatcaa
     4981 cccgcctagt gccagttgcc agggcggcgc aagtttgaga ctcacaaatc cacgatgaca
     5041 ggcgttcatt aacgttaaca acagtggtcc gcacagacct gccagcacac ctgtactgat
     5101 aatcagcgca tagtcacgag cctgaaccgt cactgagagt tgtacgttgt agagtaacgc
     5161 gtcgctatga ttaatcagat tgctaaccag caatgccacg acggcggaaa taatcaccgg
     5221 gccgagagag gccaacatca tagtgccaaa cagcacttcg gctataaata aactgccagc
     5281 aagcggggca cgataggccg cagccattcc cgccgcggcc ccacaggcga tccataattt
     5341 ccattcctgg cgtggcgtaa aacgttgggc aaaacaggag gcggcaaggg cagctaaaag
     5401 aatcatcgca ccttcgcgac caattgcact gccgctggtt actaccagca gcgaggcaag
     5461 cgatttaacc aggcttgctg cgtaatcgaa ctgtccatcg gtttgcaacg cttccatgta
     5521 atcggtcggc gcatgagggc gttgttgggt aaatttctgc cagcccatca gcaacaaacc
     5581 cgccgccagt ccgccgagcg ccggagttag cagccgtcgc cagggggaaa ggtttgtcgc
     5641 tgcattgacc agactgccgg agtcattatt gaggaacaac cactccagta gcagcatcgc
     5701 atgacgaaac ccggcaacgg caaagaccgc gagaataccg acgactgttg cgataagcag
     5761 acggcggaac atagtgcgca gatccggata gatgtgtaga tgatgcatga gcagaaatgg
     5821 gcaatgaaac acgggcgact attttgcggc aaaagaggcc aaccgcaaaa gcagtgcgtg
     5881 ctttaagaga taaaaaaacg tgacattgta acgtattatt gccgaatcgc gcgtaatcgt
     5941 caccatccgg caatattacg gtgatcctat cgaaagagtg gtttaaccgc gacaggaatc
     6001 aacagttgtg attgccattg cgccagcgtt aagcgagcca gttcacccag tgcacggtag
     6061 aaagggtgtt ctgctttttc gataaaaaca tcaagaaaac gtgttgacca cggaaaaagg
     6121 tgccatgcca gcaattcttc gcattctgtc tggcgaccat tctccgccaa ccacgcagcc
     6181 atcaacagca gtgaaccaaa atgatcttcc ggttcgtttt gcttcatttc aaactgaatg
     6241 cctttctcgc gcatccactg acgaagtgcc aatgttgaat cgccaaacag cacagattcg
     6301 cgatccagcc agaccgaccc ccacggcgga gacggcagtg cccacgggcc gacaaacaaa
     6361 cgctgccagg cctgggcgtg agtctcttca cactgtgtct gaaaagccgt caccagcggc
     6421 gctaatgacg cttctggtag aggccactga gtttcccagc cgtcactggt gagtaccgca
     6481 accagaggtg cggcttccgc actctctgga gcgtaataaa acagcgcgcc caacacgcgc
     6541 gccgcgacag aaaaattatc ttgctgtgaa aaatgggtca ttcctaacat cctgaaatgt
     6601 gcgggttgcc ccgcaccaat ttgttaacct gcaatagcca ttcctactgt catgtgcagg
     6661 ccataaaaga gcacacgccc gatcatctca ccaacaagta tgaggattag ccccagaaca
     6721 agtccggcaa cgtgaggttc acggcgacga atgagcggac aaagccagca tccaagacct
     6781 gcgcataaca ataccacacg ccagacctgt aaggaggcgt aatctggtac cagtgcgctg
     6841 gcttgttgca cggatgagtg aatagacgcc agagacaaac cttgcagcac aatcacctcg
     6901 gcacaagcga tcagcgccag aacgctaatg atggcaaatg gtgtggtatt aaatgtaacg
     6961 cgggctgccc gcagaattgc ggcagcaagt attggaccgc tcaacaatac cgtcaggaag
     7021 aaagccagcg tagtgtaacc gttatgccag gttggcacgg tgtcgatttg atacacgcag
     7081 gtcatcatcc agacgaaaat gacgccgagc gccatgctga caagtaacca gagtttcccc
     7141 aacgcttgcg gcattttacc gatgaccgcc accagccacc atagtccgcc aacggcaaaa
     7201 aatatggaac cggcagcaat ctcgttactc agaccagaag ctccgattcg attcagcgag
     7261 ttaaacgctc gcagtggcga tcccaggtgc atgacagagg caataaaccc gatgcccatc
     7321 aacagccaga ggaaaaacat gccacgaaca atgtgctgac ggtctgcgtc atttttggcg
     7381 gcgaaccagc caattccgct aacaatcagc gcgcctacta cgcattgacc caatacggta
     7441 aagataacca gtggccattc atgccatcca tttcccattt tacacttcct ccggattagc
     7501 cagataacca gtggtatcgc cggtcgggcg gctgttggcg ttaggtttga taacgatatt
     7561 gggttttgtg aaatgcgcgc gcggcagtgg cgcgacggca gccagagtgc cgtgcttctg
     7621 gcgcagctct tcaattggac cgaactcaag tgcacgcagc gggcaggatt cgacgcatat
     7681 gggttgtttc ccctcggcga cgcgcgaata acaaccatcg cacttcgtca tgtgcccttt
     7741 ttcagcattg tactgtggtg cgccgtacgg acaa
//
LOCUS       DADLSE010000164         7629 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLSE010000164 DADLSE010000000
VERSION     DADLSE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.165"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(267..4169)
                     /gene="hrpA"
                     /locus_tag="JN692_003540"
     CDS             complement(267..4169)
                     /gene="hrpA"
                     /locus_tag="JN692_003540"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7265744.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4370..4966
                     /gene="azoR"
                     /locus_tag="JN692_003541"
                     /pseudo
     CDS             4370..4966
                     /gene="azoR"
                     /locus_tag="JN692_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
     gene            complement(5020..6330)
                     /locus_tag="JN692_003542"
     CDS             complement(5020..6330)
                     /locus_tag="JN692_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HAY7265745.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWSHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKLDQRRIK
                     IALPYVVGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKL
                     PPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDVTFEF
                     PRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALV
                     VAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6320..>7629)
                     /locus_tag="JN692_003543"
     CDS             complement(6320..>7629)
                     /locus_tag="JN692_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HAY7265746.1"
                     /translation="ARPALALWHRLRGLFFINSYVKGRYLTHDRQRVASFNNDPLITR
                     AIAVNILLDLYKTSERIVSDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKEL
                     HLLPGFYHDTLGEENRAQAFEKMQSFICRLYANKSQKFDYQHEDRTGPSADRWRLLSG
                     GPVPLSPVDLAYRFMRKAMKLFGAHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFG
                     RLIDKIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDAL
                     ANEPAVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLA
                     IVSGLYELFPENEQVKNSLAGLANAIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKP
                     WVMRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT         1860 a         2086 c         1841 g         1842 t
ORIGIN      
        1 ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagcgg agcactctga
       61 aaggaggaag tcgtatttcc ttaaccgcca ctccgcaagc ctgacatatc ttttaaggaa
      121 atgctgtaga taaacattat tcatcttgca gctgagaatt gtatttttct gaattcctga
      181 atttttgtcc ttaccgtttt agaattaata aaaaacccgg cgctaaatgc ttaccgggtt
      241 ttagatttat taggcaaata gcagggttaa ccgctaatct gctccatcgc ctgcaaaata
      301 cgcttatctg aaatcggata aggcgtacca agttgttgag cgaagtaact aacgcgcagc
      361 tcttctatca tccaacggat ctctttcacg tcttcatcct cacgacgtgc aggcggcagt
      421 ttgttgatcc attgctgcca cgcctgctgg acgttttcga ctttcagcat ctgtgcacga
      481 tcgcgatgtg gatcaaccgc cagtttttcc agtcgttttt caatcgcctg caaatatcgc
      541 agcgtgtcgc ccagccgttt gaagccgtta ccagtgacaa aaccgcgata taccaacccg
      601 cccatctgcg ctttaatgtc agaaagcccc agtgccatgg tcatatccac ccgccctttc
      661 agacgtttgt tgatattgaa caccgcagtg aggatttgct cgacctgctt cgcaatatcc
      721 accaccgtgt cattcagttc ggcgcgcact ttttcatgca gcgcagcaaa gccttcttcc
      781 gtccagaccg ggccaccatt ggcgtcgatc agttgatcca caccgcagga gatacagtcg
      841 tcgatcagct ccagcacttt gccatacggg ttaaagtaca gccccagctt ggctttgttt
      901 ggtaacttct catgtaaata cttgatcggc gaggaaatat ttagcagcag tagacggcga
      961 agaccattcc acattgcctg tttttgctcc agcgggttat caaacagttt gatcgccacg
     1021 ctgtcgcgtt catccaccag cgccggccac gccttcactt tatagttgcc acgcttctgt
     1081 tcgtaacttt ccggcagctg accaaaactc cagatatgta agccgctctg ctcgataccg
     1141 tcatccgcca ccgcggacag cgtttcctgc actttgcctt tcagcgcatc tttcagatct
     1201 tgtagcgagc gcccttcttt tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt
     1261 ttcaggtgat cgggcacctg atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc
     1321 atccgccgta actcgcgctc aaggctgtcg agcaacggta actccagcgg tgtgacgcgg
     1381 cctaaaaacg cttcggcgta gtttggtgca ggcacaaaat tacggcgtac cggtttcggc
     1441 aacgatttaa tcagagcaat caccaattcg cggcgcagac cggggatctg ccattcaaac
     1501 ccgctttcct caacctggtt aagtaacggc agcggaatat gtacggtcac accgtccgcg
     1561 tccgcgcctg gctcaaactg atagctcaaa cgcagcttga gattgccctg atgccagaag
     1621 ttcgggtaat ccagcttgct gattttttcc gccccttctt tgatcaacat gctcttttca
     1681 aagttgagca aatcaggcgt ttcgcggctg acttttttcc accagctatc gaagtgacga
     1741 gcggagatta catcgtggct gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca
     1801 accagaatat cgcggcgacg tgatttgtgt tccagctctt ccacttcggc ccgcagtttc
     1861 aggttttcac ggaagaatgc gtgacgcgtc tgccagtcac cttccaccag agcgtggcga
     1921 ataaagagtt cacgacataa cgccgaatcg atctggctgt agttgacctt gcgcgcagca
     1981 acaatcggca aaccataaac agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt
     2041 tcccagtgcg gttcgctgta ggtgcgttta atcaaatgct gagcaacggg ttctacccat
     2101 tccgggtcga tacgcgcagc aatgcgcccc cacaggcggc tggtttctac cagttccgcc
     2161 accattaccc atttcggcgg ctttttgaat aaaccagaac cggggaagat ggagaaacgc
     2221 gcgttacgtg caccggtata ttcctgttta tcggcatctt tcatgccgat atgggaaagc
     2281 aaaccggtca gtaaggcgat gtgaatttca cgatactccg ccggttcgct gttgaccgga
     2341 atgccaagtt ctttcaccac ctggcgcaac tgggtgtaga tatcctgcca ttcgcgcacg
     2401 cgcagatagt tgaggtaatc ggtacgacac aggcgacgga aggcgttgga agaaagcgcc
     2461 ttttgctgct cgccaagata attccacaga tttacaaacg cgaggaagtc ggattctttg
     2521 tcgtggaagc gacgatgttt ttcgtccgat gcctgctgtt tgtccatcgg acgttcgcgc
     2581 ggatcctgaa tggagagcgc ggacgtgata atcatcgcct cacgcacgca gccatgtttt
     2641 tgcgcttcca gcaccatacg cgccagacgt gggtcgacag gcaactgcga gagctggcga
     2701 ccgagcggcg tcagtttata ggcgctggcc tgttcatccg tggtgatcgc gcccagttct
     2761 tcgagcagac gcacgccatc ctggatattg cgtttatccg gtgcttcgac aaacgggaac
     2821 gcagcgatat cgcccagccc cagcgcggtc atctgcaaaa taaccgaggc caggttggta
     2881 cgcagaatct ccggatcggt aaactccggg cgcgagagga aatcgtcttc ggaatagaga
     2941 cgaatacaga tcccttcgga cacacgacca cagcggcctt tacgctgatt ggcagacgcc
     3001 tgcgaaatgg gttcaatcgg caaacgctgc actttggtgc gatagctgta gcggctgata
     3061 cgcgctgtac cggggtcgat gacgtattta atccccggta cggtcagcga ggtttccgcg
     3121 acgttggtcg ccagcacaat gcgccgtccg ctgtgcgact ggaacacgcg gttctgttcg
     3181 ctgttcgaaa gccgcgcata aagcggcaag atttcggtat ggcgtaagtt cagcttgttc
     3241 agcgcatcgg cggtatcgcg aatttcccgc tcgccgctca taaagatcag aatgtcgccg
     3301 gggctttcct ggcttagttc gtctacggcg tcaaaaatcg cctgcaactg atcgcgctcg
     3361 gtgtcatcgg cttcttcaac aatcgggcga tagcgcactt ccaccggata ggtccgaccg
     3421 gagacttcaa taatcggcgc attattaaag tggcgcgaaa agcgttccgg gtcgatagtc
     3481 gcggaagtga taatgatttt taggtcagga cgccgcggca gcaactcttt caaatagccg
     3541 agcagaaaat cgatattcag gctgcgttcg tgcgcttcgt caataatgat ggtgtcgtac
     3601 tgcatcagca ggcggtcttg ctggatctcc gccagcagga tcccgtcggt catcagcttg
     3661 accatcgtgt tatcacttac gtgatcgctg aaacgcactt tgtaaccgat gcaaccgccc
     3721 ggctccgttt tcagctcttc cgcaatacgg ttggccaccg ttcgtgccgc caggcgacgc
     3781 ggctgggtat gaccgatcag ccctttaata ccgcgcccca gctccataca gattttcggt
     3841 agctgagtcg ttttaccgga gccggtttcc ccggcgacga tcaccacctg gtgatcacga
     3901 atcgcttcga gtatgtcctg ttttttctga ctaaccggta aattgtcagg ataagtgatt
     3961 tccggtcgtg ccgcttcacg cagcaggact ttccctgccg cctggtcaat ctctttcgcc
     4021 atctcctgga aaatggcctg ttgtgcatca ggatttttaa ccttcttaac acaatgcaga
     4081 cggcgagaaa aacgcagtct gtcgcgcagc atcagcgaat ccagccgctg ttgcaaggca
     4141 gtaaaggtca atttttgttg ttctgtcata acgttagtgg gcagtagtct gcctgattaa
     4201 ttttctttta aatgatagat atagagtacc acatcgcggc tttctatgcg ttgttcaata
     4261 aattcgaaca tagacttcga tttattgcgc tatctctgtg gcaggattgt gaataaagtg
     4321 tcagcaagca acggggcatc gtccattcaa acatctataa ggaaacacca tgagcaaggt
     4381 attagttctt aaatccagca tcctggcagg gtactctcag tctaatcagt tgtccgatta
     4441 ttttgttgaa caatggcgcg aaaagcactc cgctgatgaa atcaccgttc gcgacctagc
     4501 tgcaaatccg attccggtac tggatggcga actggttggc gctctgcgtc cgagcgatgc
     4561 gccgctgact ccgcgtcagc aggaagctct ggcgctttcc gatgagctga ttgccgagct
     4621 gaaagcccac gacgttatcg ttattgcggc accgatgtat aacttcaaca tctcaactca
     4681 gttgaaaaac tattttgacc tggttgcacg cgcaggcgtt actttccgct ataccgagaa
     4741 cggtccggaa ggtctggtaa cgggtaaaaa agccatcgtt attaccagcc gcggtgggat
     4801 ccacaaagat ggaccaacgg acctggtgac gccgtatctg tccacggtcc tcggctttat
     4861 cggcattacc gatgtgaaat ttgtcttcgc cgaagggatc gcatacggtc cggaaatggc
     4921 agcgaaagca cagtctgacg caaaagcagc catcgacagc attgttccgg acacggtctt
     4981 gatgtggact gtagatattc agtccacatc tcaatccact tacctgtttt cccataattt
     5041 caacatcgct ttgtgctctt ccttaagcac aatatgcgag cgtctggctc gaataaagct
     5101 aatcgcttca tcaactgttt tacagtgtcc gtaacataac agccatgccg ccactaccag
     5161 cgcactgcgc gataatccca atgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa
     5221 tgtttccagc atcgccacgg cctgtcggag ctccccctct tccggaacca ccagatccaa
     5281 catcggtaca cagaaataga gtcgatcttt tgtcgctcgt ccacgaggga attcaaaggt
     5341 gacgtccaga accgcattct gtgccggaat atgtcgtgga aacgccctta aataaacacc
     5401 agcagtcatt ttgctcaccg gctccaggcg acgacaaaac caacgcatag atagccacat
     5461 cccgatgcgc cagggcaatg tcagccagta aacggcgggc gggagtttcc cctgactatc
     5521 tttccctgtt gttatcgcgc caagcccacc gtacccacgg ccaataatga gtagcgataa
     5581 tactggccaa cataaccaga ctgaccacca taactgaacc atcatcatta gctccatcaa
     5641 cacaatgcac gcgcctacga catatggcag tgctattttg attcgacgtt gatcaagttt
     5701 ctgataattc caacgacggt cgacgggcac catccagtca atcaacatac ctaccgccag
     5761 ccctgtgatg acatcaataa aatgatgctg ccatgtcgtc agcgtcgaaa tggcgatgag
     5821 taaaaaccat ccgccacaga ctttacgcca cctcacagcc agatgctgac gaaagtgact
     5881 ccaaagtagc cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa
     5941 cagttcaagt tgcgaaaata gccatcctgt caccccactc acttcaggac ggataaaact
     6001 aaacttcagc gggtagagaa aaaaaccgca gcaggccatt accgttgcca gaataagccg
     6061 gtggacaagt cggcgttgtt cgaatgtcga gctacaaacg aacagcgaaa atccatataa
     6121 aagatccaga ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca
     6181 accgaatacc tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt
     6241 aagagatcca taggtgaaga agaaaaacgg ggccagcaac aataaccagc cagcgccttg
     6301 tagcaatacg ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatgc
     6361 cccacacatc aatccgttga gtgcatttat caaatccggc attacgcacg agcgaatcca
     6421 tctccccttg cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca
     6481 ccccggcaat cagctccagt tgtgggtgcc acggctgccc ggtgtagatg agaatgccac
     6541 ccggttcgat ggcattggct agacctgcga gtgagttttt tacctgctca ttttcgggaa
     6601 aaagctcata taggccagag acaatcgcca gcgtaggccg cggagttaac gcgctgagtt
     6661 cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg
     6721 caatcatctc ttgcccctgt gcaacattta actcactgta atcacgtaac aaaatatcgc
     6781 ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg tccatgccct gcggcaatgt
     6841 caaccacgcg gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcaata
     6901 tttgcaaatg ggttttgcgc tggcgaatac cgcgccatcc aacactgttc aggtagattt
     6961 tgtcgattaa acgcccgaat gcgttactgc cttgcggttg attttgatag acataatcca
     7021 gggaactgcc tgaatcaaag ccggtactca ttccgagatg caggcccgca gagtgcgcac
     7081 cgaacaattt catcgccttt cgcataaagc gatacgctaa atcaaccggc gataatggca
     7141 cgggtccacc agaaaggagc cgccatcgat ccgctgatgg tcctgtgcgg tcttcatgct
     7201 gataatcaaa tttttgcgat ttgttagcat ataagcggca gataaagctt tgcatttttt
     7261 caaatgcctg cgccctgttc tcttcaccca atgtgtcgtg ataaaagcct ggcagcagat
     7321 gcagctcttt cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca
     7381 ccacatagtc atcacctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatcgc
     7441 taacaatacg ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg
     7501 tgatcagcgg atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgcc
     7561 ctttcacata ggaattaata aaaaatagac cacgcagacg atgccataac gccagcgcag
     7621 gacgtgcca
//
LOCUS       DADLSE010000165         7586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLSE010000165 DADLSE010000000
VERSION     DADLSE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.166"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            21..4892
                     /gene="traI"
                     /locus_tag="JN692_003544"
     CDS             21..4892
                     /gene="traI"
                     /locus_tag="JN692_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7265747.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQVIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRDRGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSTGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            4912..5658
                     /gene="traX"
                     /locus_tag="JN692_003545"
     CDS             4912..5658
                     /gene="traX"
                     /locus_tag="JN692_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7265748.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMSLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            5713..6273
                     /locus_tag="JN692_003546"
     CDS             5713..6273
                     /locus_tag="JN692_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7265749.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            6404..6616
                     /locus_tag="JN692_003547"
     CDS             6404..6616
                     /locus_tag="JN692_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7265750.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            6800..7321
                     /locus_tag="JN692_003548"
     CDS             6800..7321
                     /locus_tag="JN692_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7265751.1"
                     /translation="MLHYSGGLKYRCHLSDMENNMRKYIPLVLFIFSWPVLSADIHGR
                     VVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQ
                     RDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSD
                     ADPVPPWIWRHRK"
     gene            7367..7576
                     /gene="hha"
                     /locus_tag="JN692_003549"
     CDS             7367..7576
                     /gene="hha"
                     /locus_tag="JN692_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7265752.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
BASE COUNT         1802 a         1890 c         2434 g         1460 t
ORIGIN      
        1 cggacggaca gtcagaaacg gtgctgaccg gtaatctggt gatggcactg tttaaccacg
       61 acaccagtcg cgatcaggaa ccacagttac acacgcatgc ggtggtgact aatgttacgc
      121 agcataacgg cgaatggaag acactgagca gtgacaaagt ggggaaaacg gggttcattg
      181 agaatgtgta cgctaatcag attgcctttg gcaggctcta ccgggaaaaa ctgaaagagc
      241 aggttgaggc gctgggctat gaaactgaag tggtcggtaa acacggtatg tgggaaatgc
      301 cgggtgtacc ggtggaggcc ttttccggac gctcacagac tatccgggag gccgtcgggg
      361 aagacgcctc gctgaaatcc cgggatgtgg cggcgctgga tacgcgtaaa tccaaacagc
      421 acgtcgatcc tgaagtcaga atggccgagt ggatgcagac gctgaaggaa accgggttcg
      481 acatccgggc atatcgtgac gcagcggaac aacgggcata tacccgcacg cagacacccg
      541 gacccgcttc acaggacggg ccggatgtgc agcaggcggt gacacaggcc attgccggat
      601 taagtgaacg caaagtgcag tttatgtata cggacctgct ggccaggacg gtcggcatac
      661 tgccgccgga aaatggtgtg attgaacggg cacgcgccgg tatcgatgag gccatcagcc
      721 gtgagcagct tatccccctc gatcgtgaga aggggctgtt cacgtccggg attcatatgc
      781 tcgatgagct gtcagtccgg gcactcagtc gtgacatcat gaaacagaac cgggtgaccg
      841 tacatccgga gaaaagtgtc ccccggacgg ccggttacag cgatgccgtc agtgttctgg
      901 cacaggaccg tccgtcgctg gccattgtgt ccgggcaggg cggtgcagcc gggcagcgtg
      961 agcgggtggc tgaactggtc atgatggccc gggagcaggg gcgggaggtg cagattatcg
     1021 ctgctgaccg tcgctcgcag atgaacctga agcaggatga acggctgtcc ggtgaactga
     1081 taaccggacg tcgtcagttg ctggaaggca tggccttcac gccgggcagt accgttatcg
     1141 tcgaccaggg cgaaaaactc tccctgaaag agacgttaac cctgctggat ggtgccgcac
     1201 gtcataacgt acaggtcctg ataaccgaca gcgggcagcg aaccggtaca ggcagtgcac
     1261 tgatggccat gaaggatgcc ggggtgaaca catatcgctg gcagggggga gaacagcgac
     1321 cggccaccat catcagtgaa ccggaccgta atgtccgcta tgcccggctg gccggagatt
     1381 ttgcggccag cgtgaaagcc ggagaagaga gcgtggcaca ggtcagcgga gtacgggaac
     1441 aggccatact gacacaggtc attcgcagtg agctgaaaac acagggcgtg ctcggacacc
     1501 cggaggtaac catgaccgcc ctgtcaccgg tctggctgga cagccggagc cgttatctgc
     1561 gggatatgta ccgcccggga atggtgatgg agcagtggaa cccggagaca cgcagtcatg
     1621 accgctatgt gacagaacgt gtgacggcgc agagtcacag cctgaccctg cggaatgcgc
     1681 agggcgaaac gcaggtggtg cgtatttctt ccctggacag cagctggtcg ctgttccggc
     1741 cggaaaaaat gccggtggca gacggtgagc gtctgagggt gacagggaaa attcccggac
     1801 tccgcgtctc cggcggtgac cgcctgcagg tggcatcagt cagtgaagat gcgatgacgg
     1861 ttgttgtgcc gggacgggct gaaccggcca ccctgcctgt gagcgattca ccgttcacgg
     1921 cactgaagct ggagaacggc tgggtggaaa cgcccgggca ttccgtcagc gacagtgcga
     1981 cggtttttgc ctccgtcaca cagatggcaa tggacaatgc caccctgaac ggtctggccc
     2041 gcagtggtcg tgatgtccgg ctgtattcct cactggatga aacccgtact gcggaaaaac
     2101 ttgcccgcca tccctccttt acggtggttt ctgagcagat aaaggcgcgt gccggtgaga
     2161 cattgctgga aaccgctatc agtctgcaga aagccgggct tcacacgccg gcacagcagg
     2221 ccattcatct ggcccttcct gtgctggaaa gtaaaaacct ggccttcagc atggtggacc
     2281 tgctgacaga ggcgaagtcg tttgctgcag aaggaaccgg ttttgctgac ctgggagggg
     2341 aaatcaatgc gcagataaaa cggggtgatt tactgtatgt ggatgtggca aaaggctatg
     2401 gcacaggcct gctggtttcc cgtgcgtcgt atgaggcaga aaagagcatt cttcgccata
     2461 ttctcgaagg taaggaggcg gtcacgccgc tgatggagag agtacctggc gaactcatgg
     2521 agaaactgac atcaggacag cgcgccgcca cccgtatgat actggaaacg tccgaccgtt
     2581 tcacggtggt gcagggctat gccggtgtgg gtaagaccac acagttccgg gcggtgatgt
     2641 cagccgtgaa catgctgccg gagagtgaac gtccccgtgt cgtggggctg gggcccacac
     2701 accgtgcggt cggggagatg cgcagcgccg gcgtggatgc acagacactg gcgtcctttc
     2761 tgcatgacac gcagctgcag cagcgcagcg gagaaacgcc ggatttcagc aacacgctgt
     2821 tccttctcga tgagagctct atggtgggca ataccgacat ggcacgggca tacgccctga
     2881 ttgcggccgg tggcggtcgg gctgtggcca gcggtgacac ggaccagctg caggccatcg
     2941 cgcccggtca gcctttccgt ctccagcaga cgcgcagtgc tgccgatgtg gtcatcatga
     3001 aggagattgt gcgtcagacg ccggaactgc gggaggcggt atacagcctg attaaccggg
     3061 atgtggaaag ggcgctgtcc gggcttgagc gtgtgaaacc gtctcaggtg ccacgtctgg
     3121 agggcgcatg ggcaccggag cactccgtga cggagttcag tcacagccag gaagcgaaac
     3181 tggcagaagc gcagcagaag gcgatgctga aaggcgaggc ttttccggat gtccccatga
     3241 cactgtatga agccattgtc cgcgactata ccggcaggac accggaagca cgggagcaga
     3301 cgctgattgt cacgcacctg aatgaggacc ggcgcgtact gaacagcatg attcatgatg
     3361 cacgggaaaa ggccggagaa ctggggcagg tgcaggtcat ggtgcctgtc ctgaacacag
     3421 cgaatatacg cgacggggag ctgcgtcgtc tctccacctg ggagaataac ccggatgccc
     3481 ttgccctggt ggataatgtg tatcaccgga ttgccggtat cagcaaggat gacgggctga
     3541 taaccctgca ggatgcggaa ggtaacacgc ggctgatttc gccccgggag gcggtggctg
     3601 aaggcgtcac gctgtacacc ccggacacca tccgggtggg gaccggtgac aggatacgct
     3661 tcacgaagag tgaccgggat cgcggttatg tggccaacag cgtctggacg gtgacagcgg
     3721 tttccggtga cagtgtcaca ctttctgacg gtcagcagac ccgtgtgatt cgccccggtc
     3781 aggagcgggc agagcaacat attgacctgg cctatgccat caccgcccac ggtgcacagg
     3841 gggcgagtga aacctttgcc atcgcgcttg aaggcacgga aggtaaccgg aaactgatgg
     3901 ccggctttga gtcagcctac gtggccctgt cgcgtatgaa gcagcatgtg caggtgtaca
     3961 ccgataaccg tcagggctgg acggatgcca tcaacaatgc cgtacagaaa ggaactgccc
     4021 acgatgtgtt tgagccgaaa ccggaccggg aggtcatgaa tgcagagcgg ctgttcagta
     4081 cggcgcggga gctgcgggac gtggcggcag gccgtgccgt tctccgtcag gcggggctgg
     4141 ccgggggaga cagtcctgca cggtttattg ctccgggacg taaatatccg caaccgtatg
     4201 tggcactgcc ggcgtttgac cgtaacggca agtccaccgg tatctggctg aacccgctga
     4261 ccacggatga cggaaacggg ctgcggggat tcagcggtga aggtcgggta aaaggcagcg
     4321 gggatgcgca gttcgtggcc ctgcagggca gccgtaacgg agaaagcctg ctggctgata
     4381 atatgcagga tggcgtccgg attgcccgtg ataatcctga cagtggtgtg gtggtaagaa
     4441 tcgccggtga aggccgtccg tggaatcccg gtgccatcac cggtggccgc gtgtgggggg
     4501 atatcccgga caacagcgtc cagccgggag ccggaaatgg cgaaccggtc acggcagagg
     4561 tactggcaca gcggcaggct gaagaggcca tccgccgtga aacggaacgt cgcgcagatg
     4621 aaattgtccg taaaatggca gagaacaaac ctgacctgcc ggacggcaaa acagagcagg
     4681 ctgtcaggga aattgccggg caggagcgtg accgggctgc cataactgaa cgggaagccg
     4741 cgctgccgga gagtgtactg cgtgaaccac aacgggtgcg ggaagcggtc cgggaagttg
     4801 cccgggaaaa tttgctgcag gagcgtctgc agcagatgga gcgggatatg gttcgtgacc
     4861 tgcagaaaga gaaaaccccg ggcggagact gatacaggaa gacaaacact gatgacaacg
     4921 gataacacga acacgacacg taacgattca ctggctgcac ggaccgatac ctggttgcag
     4981 tcatttctgg tctggtcacc cggacagcgg gatatcatca aaacggtggc actggtgctg
     5041 atggtactgg accacatcaa tctgatattc cagctgaagc aggaatggat gtctctggcc
     5101 gggcgaggag cctttccgct ttttgccctg gtgtgggggc tgaatctgtc ccgtcatgcg
     5161 catatccggc aaccggccat taaccggctg tggggatggg gaatcattgc ccagttcgca
     5221 tattatctgg caggctttcc ctggtatgag gggaatatcc tgtttgcctt tgcagtggcg
     5281 gcacaggtgc tgacgtggtg tgaaacgcgc agtgggtggc gtacagcagc ggccatactc
     5341 ctgatggccc tgtgggggcc tttgtccggc accagttacg gtattgccgg gttgctgatg
     5401 ctggcagtca gcaaccggct gtaccgggcg gaagacagag cggaacgtct ggcgctggtc
     5461 gcctgcctgc tggctgttat tccggcgctt aaccttgcca ccagtgatgc agcggcggtg
     5521 gccggtcttg tgatgacggt gctgaccgtt ggtctggtgt tgtgtgccgg aaaatcatta
     5581 ccccgtttct ggcccggaga ctttttcccg acgttctatg cctgtcatct ggctgtgctg
     5641 ggcgttctgg cgctgtgacg ggtgtggtat ctttggccgc aagaggatga tttgtcagag
     5701 gcagaacaca gcatgacaga gcagaagcga ccggtactga cactgaagcg gaagacagag
     5761 ggaacagcgc ctgtccgcag ccggaaaacc atcatcaatg tcaccacgcc accaaagtgg
     5821 aaggtgaaaa agcagaagct ggccgagaaa gcggcccgtg aagcagagct ggcggcaaaa
     5881 aaagcgcagg ccagacaggc gctgtccatt tatctgaacc tgcccacgct ggatgaggct
     5941 gtgaataccc tgaagccctg gtggccggga ttatttgacg gtgacacgcc ccggcttctg
     6001 gcctgcggta tccgggacgt gttactggaa gacgtggcgc agcggaatat cccgctctcg
     6061 cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt cagaaagcta tctgtgtgcc
     6121 atgaaagccg gtgcctgccg gtatgacacg gaagggtatg tgacggagca tatttctcag
     6181 gaggaggaag cgtatgcggg agcgcgtctg gcgaaaattc gtcgccagaa ccggataaag
     6241 gcagaacttc aggccgtgct tgatgagaaa taaaaaaagc ctcccccaga gagaggaggc
     6301 agggaaataa ggcttaaaag gaaataactt ccgcttccga aaggaagcca gtccactgaa
     6361 ttatttatca caataatact gacaactaaa gggggacaag aaaatgaatg gattcagaaa
     6421 cagttcacgg aacggccagg tctggcgtta ccagcgtgcc ggcgggcggg ccgttattct
     6481 ggaagtcagt ggacgctgga tggaagcggc agaagcatgg cgacgggctg cctgtgttgc
     6541 gcctaggaca gactggcagc agtttgcccg gaaaagggct gaacactgcc accggcgctg
     6601 ccggggaagg gtgtaacgaa aaaagcagcc ttcattactc caggagtcag aaggctgaaa
     6661 ggagtttatg agatttgctt cttaatatcc ggtcgggcag tatgaccgga cagttctgag
     6721 tagcacagag tcttttccgc attaagtcga ttttttatat tttgtgatta atttcacaaa
     6781 ataaggtgtt attcaatgtg tgctgcacta ttcagggggt ctgaaataca ggtgccattt
     6841 atctgatatg gagaataaca tgaggaaata tattccactg gtattattta tcttttcatg
     6901 gccggtattg tctgcagata ttcatggacg ggttgttcgt gtactggacg gtgataccat
     6961 cgaggtcatg gactcactga aggcggtgcg gatcagactg gtaaatattg atgcaccgga
     7021 aaagaaacag gattatggcc ggtggtcaac agacatgatg aaatcactgg tcgcaggaaa
     7081 aacggttacc gtcacatatt ttcagcgaga tcgttatggt cgtattctcg ggcaggttta
     7141 tgcaccagac ggaatgaata ttaatcagtt tatggtccgg gccggggctg cctgggtata
     7201 tgagcagtac aacacagatc ccgtgctacc tgtccttcag aacgaagcca gacaacaaaa
     7261 acgtgggctc tggtcagatg ctgatccggt tcccccctgg atatggaggc accgtaaata
     7321 aagcgatggc aatcctatca ttcatctgtt ctcagcggga ataaaaatgg caaaaacaaa
     7381 acaggaatgg ctgtatcagt tacggcgatg ttcatcagtc aatacgctgg aaaaaattat
     7441 tcataaaaac agagacagtt tatcaaacag tgaacgtgaa tcatttaatt cagcagcaga
     7501 ccaccggcta gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg
     7561 gaaatatgta aggtaataat tcagca
//
LOCUS       DADLSE010000166         7555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLSE010000166 DADLSE010000000
VERSION     DADLSE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.167"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..1542)
                     /locus_tag="JN692_003550"
                     /pseudo
     CDS             complement(1..1542)
                     /locus_tag="JN692_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1488..1709)
                     /locus_tag="JN692_003551"
     CDS             complement(1488..1709)
                     /locus_tag="JN692_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265753.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLRKAEYITARRPA
                     GQKKKHDAEHSECGTFCDEPEKTRNQDVM"
     gene            complement(1754..3691)
                     /locus_tag="JN692_003552"
     CDS             complement(1754..3691)
                     /locus_tag="JN692_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7265754.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVKGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEAMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTEFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            complement(3755..5416)
                     /locus_tag="JN692_003553"
     CDS             complement(3755..5416)
                     /locus_tag="JN692_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7265755.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYGAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(5413..5976)
                     /locus_tag="JN692_003554"
     CDS             complement(5413..5976)
                     /locus_tag="JN692_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAY7265756.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            complement(6265..6630)
                     /locus_tag="JN692_003555"
     CDS             complement(6265..6630)
                     /locus_tag="JN692_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7265757.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKHHIWLR
                     DAGQCRLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     TTLPEVSGGSGRADDIIGL"
     gene            6672..6872
                     /locus_tag="JN692_003556"
     CDS             6672..6872
                     /locus_tag="JN692_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HAY7265758.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            complement(7004..7330)
                     /locus_tag="JN692_003557"
     CDS             complement(7004..7330)
                     /locus_tag="JN692_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HAY7265759.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
BASE COUNT         1659 a         2375 c         1826 g         1695 t
ORIGIN      
        1 tcaagcatcg gacgcatatc atcgcccgtc acgcacttct gtaataccac agacagggac
       61 gccagttttt cttcaaacgt ttgtgcctgc gcggctattt tcccctcaaa tgtgcgctgt
      121 aaatccgcca gcactgcgga aaattcttca cccagcgcac ggataatgga cagttcacgt
      181 tcattcattt tttcagaatc cccctgaaca tcgccttcac cgcatcatgc tctgtttcag
      241 tgattgcctt attaccgtca gatgcgctgt caggcggttg cgctaaggcc gttttcccgg
      301 tcgacgcgaa cggatcttca cgggcatcac gacgggacag cgcctccaga ctgtagttct
      361 gctgctgaag atacagtgca tcacctcccg caaggggcgg caggttctca cgtttacggg
      421 cctcattggg cgtgagaagc gtatttttca ccgactcacc cagcgttttc atgcgccgtt
      481 cgctgtccat tctcagcagc gtggtgacgt caaactccgt actctcgttt tcccccgttt
      541 ccagcgcctc atccagtaac agttcaatgg actcaatcag cgtctgcagg cactgggaat
      601 aatactgctg ctccagcgcc tccacgttgt cactggaagg tggctggcca acgccaatct
      661 tgtaggccgg gacacggaac accgaacaga caatttcagc ggtcatcttc agttgttcca
      721 ccgtctgcgc atccaccggt gaaaacgtcg tggggttgta ttttgccccg ttgctcaaaa
      781 tggccgtttt ccccgcattt tcgccggtat acccgctgtc ccagttgctc ttcagttttt
      841 tcgcattttc ttccgtaata ctgccgggga tctcaatcac cccggacggc ctgccgccat
      901 ttctgaaaaa agacgtcgaa ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg
      961 cggcatacac cggcggcagc cccacaagcg gatgaaaaaa acagttaaac cggtcgcttc
     1021 ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg tgatgcgata
     1081 gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ctccagtcca gaatacgcag
     1141 ttctttgatc tgcccccggg agttacggat tttcagcacc accgtattgc cgtgacgcag
     1201 tttggcgttc agccacagtt caaaaaactg gatacgattc tgctgtgcat tgggacgacg
     1261 acagagacgg gcaatatccc cctgccgttt ttcacggcgg atcccctgtg tatccgtctg
     1321 catcaggcgc agccgcattt tggcgatatc ctgggatatc agcgaaatgc aagaaaacac
     1381 cgcatgaaag gagaggacac tttccggatc ggctttcaca ccctgctgcc aggcgccagc
     1441 aaagggctca gccaccgcct gaaacaggct ggtccagccc acctctttta catcacgtcc
     1501 tgatttctgg ttttttcggg ttcgtcgcaa aaagttccac attcgctatg ctccgcatca
     1561 tgtttctttt tctgacccgc cggacgtcgc gctgtgatgt actccgcctt cctcaggcga
     1621 accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg ggcatcatgc
     1681 gtgccctgca gatatcggat ctttgccata acctgttacg ggaggcgcac gcctcccgtc
     1741 ctccttatca gactcagccg ccggacgcac tgccgtagtt cactccggtg atcaccgcca
     1801 ccgccgcggt acggcgacga cgccagttga tccagcgctc cgcacggatg gccacactgc
     1861 ctgtctggaa catggaaacc agctccaccg gcgacggcgt ggtactgtcg ccggtcggct
     1921 cagactgcat ttccagtgat gcctcgcggg acatatccac tgccacgccg ccgtcatccg
     1981 ccagataaat atccggggca ttcaccagca ccagctggtc acccacgtac tgggagacaa
     2041 tcaccggcag cccctggaag gagccaccca gcagggtcat gtccgggtat tctttctgac
     2101 ccagcgcatt tttacgcatg gacagcgcca gggcattggt gctggacatc agccagacag
     2161 caccggtggg ctgcaggttt gctgccacaa actgtccaaa cgccgcctct gcatccgcat
     2221 ccgggttacc gcttgatgcc gtgcccttca catcatgggt gatggacgcc ggggagacat
     2281 ctgccaccgc cgcttttttc gggtccacaa attctgtatc cagacgcgcc accaccgctt
     2341 ccgccagcgc attacggacc agtgcatcag cagccggact ggaaaaacgg atcaattctt
     2401 ccgtcagtac cgcaatggcc gacaccttcg catgactgaa ggtgatggat tcaaaatcaa
     2461 acttcgtcag gggttttgcc ttaccctcac ccacccagcc ggcagcaccg cccgacacct
     2521 gggcgtgcac acggatattg aacggcacct gacgaagtgc agggatcccg ccctgaccaa
     2581 atcgcccgat aatggtctga ggacgcaggt aatcaataaa gtcctgtgcg tattcctgat
     2641 attcagacag gctgcctgcc cactgcggat ccgtggtggt ccccgcgccc actgccgatt
     2701 tcaggacatg atgcagacga ctgtcatccg gatactgacg acgggccact tccagggctt
     2761 cagagcggac acctttagcc gcagccagcg atttggcaaa acgtgcgaaa ccaatcccct
     2821 tatccagttt ctgctccaca cggatcaccg gcgcagaagc caccgcggcc acattcccgt
     2881 taccggcctg tttcaccggc tgcgccgtgg cggccttacc ggcttccagt tcacgcaggc
     2941 gcttcaggtg cgcatccacc tgacgtattt ccgctgcggt gttgtcgtaa tgctcttcct
     3001 cctccacatc cagcgtgcgc ccttcctctg cggctttggt catggcctcc tcaagggagg
     3061 ctgccagtgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata ttcattatgg
     3121 tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa gatgcgattt
     3181 tcaccaccgg tttccggttg ccggacgcgg cagaaaactg gcggtcgtaa gatttaatgg
     3241 tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc atcagctccc
     3301 agcgcagaaa atgcagtccg cctccgtcca gaaaggtgta ttcatgagga cggaagccca
     3361 cggacagccc cttgaccagc ccggtcttaa tggccgccca ggcctcatcc agccgggcag
     3421 ccagttgcga tggcatatcc ggcacgggct tcgccagtgt tgccgtgatt tccagccctt
     3481 cgctgacccg gcgcaccgta cactgcccca ccgggcggga atggtcatgc tgccagagaa
     3541 acgggatcgc actgccaaac tccgcgccct ccggctccag gatgtcacca tcccgatccg
     3601 gagaaggcgt tgacgcaatc ccggtgatca cccgctcatc ctcactgaag gatttcaccg
     3661 tcagcaggga gcaggcccgt ttaagagtca catcagcctc ctgaaaataa aaaaaccgcc
     3721 gcagcggtcc atgatggtta cagggtgaac agggttatat gaaaaaaacc gcatattctt
     3781 tctttttcgg ctccggattc agggacatca gggataccgc attgaagagc gccatcagcg
     3841 ggtcaatttt cccccgcccg ctggcctgtt tggtaataag gatagcgtta cctttaggct
     3901 ccacccgggc attgccaacg caccaggcca tcagtggctg accaccatgc accagtactc
     3961 cctcagccag tttgcgctcg gtggttttta tggccccgcc cagcttccag ccctggctta
     4021 tccccacaac aattccgtcg gggatcccgg cttccgccag tgaatccaga atctgcccca
     4081 cacctgacgg gtcaataccg atatgctcca gtaactcagc ctcatgaata cgacgcacat
     4141 actccgccac ttccgccgtg tcatccccga cccgacggac aatcgtcatg tctccacagg
     4201 ccacaaaatc ctgaaaacgg gatgcctcac tcttccgtct gaccaccgcg gtttcatgcg
     4261 cccaggcatg gccccagccc agccattcgc gggtttccct gtcacggcca atcacgtaca
     4321 ttcccagcag atcatccagg cccccgccgt caatccccac cgtcaccaca tcagcgcgct
     4381 gcaggatatc gtccaggctg acgcgcctgc cctgctgctc ccagaaatcc gcacccgccc
     4441 agcggtcaga acgcagggca agaccaattt ccacattggc atgttttgac atgaagccac
     4501 gaaatgcttc ctcaccagcc tcccgggctt tacggtactc ccggtacaga aaagcctcat
     4561 ccaccgaata accgagattc ggattgacca tggcgaggtt ttccatcagc aggtgagccc
     4621 cgctttccac catttcagga gggtgttcaa atatcaccgg cagaaagtgc ggatcatgaa
     4681 ttttgccgtc acggacatcc cgggcgtact gcagtttctg tctgaacacc ccggctggcg
     4741 gctcattcga ctgggtggtc gtatacacca caaacccttc cgggcgggag gcaaggccgc
     4801 ctatggcttc acgtaacatg tcctccgctt tgtactgctt gccaaataac cacagttcat
     4861 caatcagcgt ccccacggac ttgataccgg ataccgtatt cggatcggca gccaccacct
     4921 taagggtggt gtccgtcacc ctgtgggtga tggtccggat atgtgtctgc acctgacaga
     4981 ggtcatccag atcatcgtcc cgtcgtacca tatccctagc agggttgaag gcgttagccg
     5041 ccacctccac ggtcggggcc agaatggtat agcccgccgc ctgccgccag ttcagtaaca
     5101 gcgccgtcat catgatcccg gcagccagcg tggacttgct gtttttcttg gggataagga
     5161 taaaaacttc cttgatatgg cgtacaccgg tctgcgcacc gtaggagcca aacagggccg
     5221 ccaccaggtc aaacacccac tgtgcgcagg actccccgaa cgtcgggctg cccggtgcat
     5281 ccacaatccg cagttgttta aaaatcgcca gggcatgtgc ggcctgctcc ggataaatcg
     5341 gagccggaat aatcgacagc ccctttttca ggcgctctgc ccagtccggg caggccgtgc
     5401 tccacacagg tatcatccgt tgccctcatt atcattattc accaccaggc ggggtggtgg
     5461 tggcaccgca aaacggttag ccgctttttt cgccgcgtca ccttttgccg attttttacc
     5521 ggcatcccct tttttgtggt gcgtgaactg cgccagctta taagccgcat ccagcgccag
     5581 cctggggtcg gtattaatgt tctccaccag aagacgcccc atcgctttca ccggatcggg
     5641 aagaccgtcc tccatatact caataccagg agatatcacc acgggcggtg gcatctccgg
     5701 atttgtttcg tccggctgtg gtattgcagc cgcctcacgg cgacggggtt tatcctccgg
     5761 ctctgatttt ttctgccgat aaacaggaac ctcatccacc tccaccgtct cgcactgttt
     5821 acgggctata aacgcaagca cctccggatc ttttgccagc tgcgagcctt taaccctggc
     5881 tgtcttcgcc gaataaccgg cggcaagggc tgacgctgtt ttgtttttcc cggacatgag
     5941 cgccagcgca aattttcgtt tttgcgttgt cagcacagcc tcctcccggg tccagaacgc
     6001 actcagccgg gtatggttca gcccattttt cccggcgtct catgccgcaa atgttaactg
     6061 ctgcctggtt aacatttgct gaaaaatcct gttaacattt tttccacgca acaaactgaa
     6121 taataaagat aaaaaccgca aaaatgcccg gacagccagt taacatgtta actgccctga
     6181 aacgggaatt ttttctctgc gtgagagggg gcgcggtgtc cggagcgatc gttttttacg
     6241 ccgaatgata cccccccggt cgggttacag tccgatgatg tcgtccgctc tgccactacc
     6301 tccggacacc tccggcagcg tcgtgtccgg cataccaccc gccgcttcac gagcagactt
     6361 ttgtcgatgg cattcggtac agagcgtcca gagattcgtc tcctcattac caccaccgaa
     6421 ctgaagtgca attcggtgat cgagttcact gtcacagagg tcaaccacac gaccacagag
     6481 acggcactgc ccggcgtccc tgagccagat atgatgcttg agggaaacac gtgcactgcc
     6541 actgacacga cgctgttcac ccttcagaat attcacccgt cgggtattca gtgttttgat
     6601 tctgctctgg agtgtacgaa gctcagccat gtaaaatccc cgtcatatgg caatcagtaa
     6661 aggaaataaa tatgtcatcg aaaaaccgta cccgcagaac cacaacccgc aatatccgtt
     6721 tccccaatca catgattgaa cagatcaaca tcgcccttga gcataaaggg tccggtaact
     6781 tttcagcgtg ggttattgaa gcctgcagga gaaggctggc aacagatgca acgcatctgc
     6841 gcccggccag catgacaaat aacgagaaat gaacgttcgg ttacaggagc aggtacccac
     6901 tgtcctccaa caatatttca tcttcatatc cgacggaaca agacttaccc tgccgggatg
     6961 tacagaataa caacagagtg ataattaatt tctgatgaaa taatcagggt gcagaaggac
     7021 taaagataaa cgttttcttc acgcctttac acggcctgtc cttctcaaat cgccattttg
     7081 ccatcgcctt tacaacctgc tcatcaaaca gatggtgcgg ctctgaacga ataaactcaa
     7141 ttcgggtgac agtaccatca gcaccaatat caaacctcac atcaacccgt ccctttatat
     7201 aatttgccgc tgcataggcc ggatattgtg gtaatgcctt aaccaactgt cggggcatat
     7261 ctgttttatg ttgcgtacag cccataacca gagaagacaa caaaataatt aacggaagat
     7321 ttcttttcat tttcattccc cgcacagata agaataagtc ttattctaac aatgccgccc
     7381 tgtcggtcat caatcctctg cttaatggca acgacaatta tccgacttaa atcacaaatc
     7441 agacacatga cataacaggg cttgcgaggt aacacatcgt cctgtttctt ccaccatcgc
     7501 accggactgg cgactatgag gggacaacgc cgcgctccgt taacgcggta aaccc
//
LOCUS       DADLSE010000167         7528 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLSE010000167 DADLSE010000000
VERSION     DADLSE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.168"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            556..1326
                     /locus_tag="JN692_003558"
     CDS             556..1326
                     /locus_tag="JN692_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7265760.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="JN692_003559"
     CDS             1317..1790
                     /locus_tag="JN692_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7265761.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="JN692_003560"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="JN692_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7265762.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="JN692_003561"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="JN692_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7265763.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="JN692_003562"
     CDS             3225..3719
                     /locus_tag="JN692_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7265764.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3973..6264
                     /gene="opgB"
                     /locus_tag="JN692_003563"
     CDS             3973..6264
                     /gene="opgB"
                     /locus_tag="JN692_003563"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7265765.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6402..7424)
                     /locus_tag="JN692_003564"
     CDS             complement(6402..7424)
                     /locus_tag="JN692_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7265766.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
BASE COUNT         1914 a         1951 c         1833 g         1830 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccagg
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ctgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct gcccttttcc
     3781 ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa aaaagtctgt
     3841 ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca gcaatatggc
     3901 gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc ccggtttagt
     3961 gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc tgtgctgatt
     4021 tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacgttaac ggtgctgggg
     4081 ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg cgatggtatt
     4141 aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt cagcaaatac
     4201 atcctgccgg gtatcggcat tgtgctgggg ctgacggcgg tgttcggtgc gctgggctgg
     4261 atcctgcgcc gtcgtcgtca tcatccgcac cattttggtt acagcctgct ggcgctctta
     4321 ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga actggtgaaa
     4381 tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc gtcgaaaact
     4441 atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga gcggacctat
     4501 tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa aaatgaaggc
     4561 ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc gggcatggtg
     4621 gcttctcagt gcggcatacc gctgttcgcc ccctttgaag gcaacgcctc cgcctctgtc
     4681 tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc gggttatcag
     4741 aactatttcg tgcagggcgc aaatctgcgt tttgccggta aagatgtgtt cctgaaatcg
     4801 cacggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtagc cgacccgcac
     4861 tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg gaaaaagttt
     4921 gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt cgatacccat
     4981 cacccggatg gttttatctc gcgcacctgt aaccgcaaaa aatatgattt tgacggtaag
     5041 ccgaatcagt cattcagcgc ggtaagttgc agccaggaga atatcgcgac gtttatcaac
     5101 aaaatcaaag cgtcaccgtg gtttaaagat actgtcatcg tcgtctcttc tgaccattta
     5161 gcgatgaaca acacggcgtg gaaatacctc aataagcagg atcgcaataa cctgtttttt
     5221 gtcattcgtg gcgacaagcc gcagcaaaag acgctggcag tgaagcgtaa cacgatggat
     5281 aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact tggtcgtagc
     5341 agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa aacattggcg
     5401 tggaagccag atatcatccg cctgtggaaa ttccctaaag agatgaaaga gttcaccatc
     5461 gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc gctgctgttg
     5521 cgggtttcag acaaacgcgt ggaaccgctg ccggaaagcg aatactcagc accgctgcgt
     5581 ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgacag ttgctacaag
     5641 atggcacaac tctgggctcc ggaactggca ctttccaccg actggtgtgt ctcgcagggg
     5701 cagcttggcg gccagcaaat tgttcagcat gttgacaaaa caacatggaa gagcaagacg
     5761 gcatttaaag atacggtcat cgacatggcg cgttacaaag gcaatgtcga tacgctgaag
     5821 attgttgata acgatattcg ctacaaagcc gacagtttca tctttaacgt cgccggtgcg
     5881 ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg ccgctggtcc
     5941 aacgcgcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc gaagaaattt
     6001 gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat tccggtacgc
     6061 gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag tgaccaccac cacactgcat
     6121 ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc tgtctcaacc
     6181 aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat ggtggaaatt
     6241 aaagtggtag aacgtgaagg gtaattcctg actgatgtgt aggtcgcata gggtgcgacc
     6301 tacacgcctt ctccggctta caacacatta ttaagaaaca aaaaaaacag cgcccaacac
     6361 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6421 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6481 ggatagcgat gggttaacat catgtcggca gtgattttcc cttccgccat cagtcgaccg
     6541 actttagcga aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6601 tggaattccg gatcggagaa ctgcaactca cctttaaaca gaccgacaaa taccaccgtg
     6661 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6721 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6781 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6841 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6901 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6961 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     7021 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     7081 tgcacgccaa tgactgaaat tttttcacag cagttggtac gcccgctttt acacgccggg
     7141 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     7201 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     7261 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7321 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7381 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7441 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7501 gtgaaaacac acgcataaaa atgtttta
//
LOCUS       DADLSE010000168         7517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLSE010000168 DADLSE010000000
VERSION     DADLSE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.169"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1064)
                     /locus_tag="JN692_003565"
     CDS             complement(<1..1064)
                     /locus_tag="JN692_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7265767.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDR"
     gene            complement(1643..2320)
                     /gene="bioD"
                     /locus_tag="JN692_003566"
     CDS             complement(1643..2320)
                     /gene="bioD"
                     /locus_tag="JN692_003566"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7265768.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTV
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGELPWLAENPENAATGK
                     YINLALL"
     gene            complement(2313..3068)
                     /gene="bioC"
                     /locus_tag="JN692_003567"
     CDS             complement(2313..3068)
                     /gene="bioC"
                     /locus_tag="JN692_003567"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7265769.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(3055..4209)
                     /gene="bioF"
                     /locus_tag="JN692_003568"
     CDS             complement(3055..4209)
                     /gene="bioF"
                     /locus_tag="JN692_003568"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7265770.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(4206..5246)
                     /gene="bioB"
                     /locus_tag="JN692_003569"
     CDS             complement(4206..5246)
                     /gene="bioB"
                     /locus_tag="JN692_003569"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7265771.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            5333..6622
                     /gene="bioA"
                     /locus_tag="JN692_003570"
     CDS             5333..6622
                     /gene="bioA"
                     /locus_tag="JN692_003570"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7265772.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGTMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            6681..7157
                     /locus_tag="JN692_003571"
     CDS             6681..7157
                     /locus_tag="JN692_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7265773.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         1713 a         2061 c         1952 g         1791 t
ORIGIN      
        1 cggtcaccgc gatacatgcc gccaatttgt ggaatggtga cgtgagattc atcgacgacc
       61 agcagcccat cggcaggcag gtaatcaaac agcgtcggcg gtggctcacc cggtccacga
      121 ccggagagga agcgcgagta gttttcaatc cccgaacagt agcccagctc gttcatcatc
      181 tccagatcaa actgggtacg ctgggtcagc cgctgctctt ccagcagttt gttgttttcc
      241 aacagcactt tgcgtctggc ggccagttct tctttgatct cctccatcgc ctgtacgatg
      301 cgctcgcgcg gtgtgacgta gtgcgttttc gggtagatgg taaaacgtgg aatagtggaa
      361 acaatctgcc cggtcagcgg gtcaaataac gacaatcgtt ccacttcctc gtcaaacagt
      421 tccacgcgaa gtgcaatgtc atccgattct gccgggaaga tatctatcac ctcgccacga
      481 acgcggaaag taccacgctg gaatgcttga tcattacgag cgtattgcag ctccgccagc
      541 cggcgcagaa tcgcgcgctg atcgataatc ataccgaccg tgagatggag catcatcttg
      601 agatataaat caggatcgcc cagaccataa atcgcggaaa cagacgccac tacaatcaca
      661 tcacgccgct ccagcatcgc tttggtggcg gacaaacgca tctgctcaat atgttcgtta
      721 accgaggcat ctttctcaat gaaggtgtcg gaactcggta cataggcttc cggctgatag
      781 tagtcgtagt aggagacgaa atactccacc gcgttttccg ggaagaactc tttcatttcg
      841 ccatacagct gggccgccag cgttttgttg ggcgcaagta ccatggttgg gcgctgaagg
      901 tcagcaatga cattggcaat ggtgaaggtt ttcccggagc cagtcacgcc aagtaacgtc
      961 tggtgcgcca ggccatcttc cagcccctct tcgagacgtc gaatcgcctc tggctgatcg
     1021 ccagaaggtt taaaagcgga attcagtttg aacggtttac tcatgagtcg ctacctgaag
     1081 gagttgggcg ggcaggtatg taattttact cgtcgtactt aattatgcca acaaattata
     1141 ctggataaaa aaacagttca tcaccataat atttctgata cagcgtaaac tccgctgtca
     1201 atcatgagca aaatttactc tgtggcgaga taaaactccg gccttaccgg gttatcccca
     1261 aagcaacggc ttttttaaca tttgtcaaga tgagtgatga cagttttatg gcaagagatg
     1321 cctgttcagt gactgccatt gcatttatct aaccagttaa aaaataaaag atatttcttt
     1381 gagccgtctt taacgccaat tcgcgtgcaa gccgcgtatt ctctcgcttg cctcgtgttt
     1441 tctaactcta atacacatgg ttatccacag gaatagtgga taactgtctc cagcccctat
     1501 cccccgccgc ctgggcaatt cccacattcg ccgcatgagg cggaagtaaa atttttttcg
     1561 tgattatttt agaatttaat tggttaaatt gcagtcaatc gaagacgcga tctcgctcgc
     1621 aatttaacca aatacagaat ggctacaaca aggcaaggtt tatgtacttt ccggttgccg
     1681 cattttctgg attttctgca agccagggga gctctcccag cagcggcgcg ggaatcatgc
     1741 gggtgagcgt ggtcatatat tcagcgtgac gttttcccgg aggcgtaaca tcgttcgcca
     1801 cccaacccgc cagagtcagt ccggcgtgtt gtattgcctg cacagtcaac atcgcgtgat
     1861 taatacagcc gagtttcaca ccaactacca gtatcaccgg cagttgttcc tgtgttaccc
     1921 aatctgcaaa agtgaaagtg tcagaaagcg gcgtaaacca gccgccagca ccttccacta
     1981 acacccagtc agcctgttgt tcaagcgcgc gtaatccggc gctcattacc aatgattcta
     2041 tcggcctgcc ctcttgggcg ctgatgatgt gcggcgaggt gggttctgcg aaggtgtaag
     2101 gatttaccgt tgcgtaatcc agctgcaggc tgctgttgtg ctgtaacgcc agcgcgtcac
     2161 tattacgtaa gccttccggg gtcttttcgc tgccagaggc gaccggttta taacctgccg
     2221 tccgataacc tactgccttc gcggcttgta aaagtgcaca actggcgacg gttttcccca
     2281 cttcggtatc cgttccggtg acaaaataac gtttactcac gagcaatcac tcccaaaaaa
     2341 agatgatacg tcagaggata tcgcccctgt tgttgcggcc aggccagttg caatcgctgc
     2401 aactgcgaac gcgttaatat tcgcgggtcg cgcccttcat gaagatgcgt agcaccgatg
     2461 cctttcagcg aacgcatggc actgagcgca tcatcaaacc atagtgtgat gagctgaata
     2521 tgatgttgat aacgcacgcc gttcagcgac tcttcgattt catctggcgg taaaaagcga
     2581 ttagcatgcg gacgctcgtc caccgcctgc cgcgcctgat gcagttcggg taacgatccc
     2641 tgcaccagcg tggtaaacgc gaccacgcct ccggggcgca ccacccgata cagctcgcgg
     2701 agtgccgtgg ataaattacc gcaccactgc accgcgagat tgctccatgc aagatcgaac
     2761 gtcgcagtcg ctaacggcag ggattcgata tctcccgcca gataatgatc tgcggcatct
     2821 ttatggcggg cttgcgcaag cattggcggc gagagatcta acgccgtcac ctgcgcgtga
     2881 cgttcccgcc agtggcggct catccagcca ggtccacaac ccgcgtccag tacgtgggtg
     2941 tatttacgct gtggaagcat tgccagtaag gcgtcagcac tctggcgctg tagatctgca
     3001 tgttgctcat agtgtgcggc tgcccgacca aatgccgctg caatggcttg tttattaacc
     3061 gttgccatgc agcacctcca gcagacggtc gatatcctgc atttcatgcg cagcggttag
     3121 cgttaagcgc agtcgcgcag taccagcggg tacggttggc gggcgaatcg ccgtgaccca
     3181 gcagccttgc tggcgcagtt tttctgccag ttgtaacgca cggctgttat caccgacgat
     3241 caatggctgg atggcgctgc atgaatcagc cagcgtaaac ggcaaatcct gtactccggc
     3301 acgaaaacgc gcaatgagtg acaccagttt ttcgcgccgt gcatccccct catcacggcg
     3361 aatgaccgcc agcgacgcac gtaatgcctg cgcctgagcg ggcggcatac tggtgctgta
     3421 gataaggtgg cgggcgaatt gcagcagata atccgccacc gtacttgagc aaagcaccgc
     3481 agccccactg acgccaaatc ctttgccaaa agtcactacc agcaattctg gttttacctt
     3541 ttgcagccag cagctgccgc gcccctgctc cccgataacg cccgtgccgt gggcatcatc
     3601 gaccatcaac cagccattgt gctgttgcgt tacctgctgg atttccgcca gtggcgcgct
     3661 atcgccgtcc atgctgaaca cgccttctgt caccaccagt tgctgccccg gacaggggga
     3721 agcaagcagt cgcgccagat gagcgacatc gttatgagta aaacggcgaa gctgcgacgg
     3781 gcttaaactg gcagcctcca gcaatgaggc atggctaagc cggtcggcaa caatacggtc
     3841 ctctttcgcc atcatcgcgg caatgactgc ctgattcgcg gcaaaaccag agataaacag
     3901 cagcgcccgc gaatagccaa gccactcggc caattcttct tccagcgcct gatgcaccac
     3961 gctataaccg ctgacgtgac cggagccgcc gctaccgatg ccaaattgct ccgccccctg
     4021 cttccaggca cggataattt gcggatgatg gcttaaacct aaataatcgt tactggaaaa
     4081 gttcagatac tggcgatcat ccgccaccag ccagcgtccg gctccttgcg ccaccggata
     4141 acggcgacgc agggcatcgg cagcacgccg cgcatcgagc gccgcgttga ttttctcctg
     4201 ccagctcata atgctgccgc gttgtaatat tcgtcggtgt ccggggtcat cagcgcctgt
     4261 tcaagacgtt gctgttgttc gttatcccct gccagcacgg cagtttgctg cggatttagc
     4321 cccagtttgc ggaacagttg caggtcttta tcttcttccg gattcggcgt ggtcagcagt
     4381 ttgcaaccgt agaaaatcga gtttgcgcct gccataaagc acatcgcctg agtctgttcg
     4441 ttcatctgct cgcgtccggc agaaaggcgc acgtaagagg tcggcatcat gatccgcgct
     4501 acggcaatgg tgcgaataaa atcaaaggca tcaacgtcat cgttatcggc aagcggcgtg
     4561 cctttcacct tcaccagcat gttgattggt acgctttccg gcggcgtcgg caggtttgcc
     4621 agttgcagca ataatccggc gcgatctttt accgtttcgc ctaagcccac aatgccacct
     4681 gagcagactt tgatcccggc atcgcgcact ttttccagcg tatcgaggcg ttcctgataa
     4741 gtgcgtgtgg tgatgatatt gccgtaaaac tccggcgagg tgtccaggtt gtggttgtag
     4801 taatccagcc cggcgttcgc gaggcgctgc gcctgagatt cactcaacgt gcccagcgtc
     4861 atacacgcct ccagccccat cgcttttacc ccctgcacca tttgttccag gtacggcata
     4921 tcgcgttcgt ggggattctt ccacgccgcg cccatacaga agcgcgtcga tcctgccgct
     4981 ttcgctttgc gcgccgactc cagcacctgt tcaacttcca tcaaccgctc ggcttccagc
     5041 ccggttttgt agcgcgagct ttgcgggcag tatttgcaat cttccggaca agctccggtc
     5101 ttaatcgaca gcaacgtgct gacctgcacc tgacgaggat cgaaatgctg gcgatgcacc
     5161 tgctgcgctt caaacagcag atccagcaac ggtttttcaa ataattctgt gacttgcgac
     5221 aatgtccagc gtgggcggtg agccatgggg cttctccaaa acgtgttttt tgttgttaat
     5281 tcggtgtaga cttgtaaacc taaatctttt caatttggtt tacaagtcga ttatgacaac
     5341 ggacgatctt gcctttgacc aacgccatat ctggcaccca tacacatcca tgacctcccc
     5401 tctgccggtt tatccggtgg cgagcgccga aggttgcgag ctaattttgt ctgacggcag
     5461 acgcctggtt gacggtatgt cgtcctggtg ggcggcgatc cacggctaca atcacccgca
     5521 gcttaatgcg gcgatgaaga cgcaaattga tgccatgtcg catgtgatgt ttggcggtat
     5581 cacccatgcg cccgccatcg agctatgccg caaactggtg gcaatgacgc cgcaaccgct
     5641 ggagtgcgtt tttctcgcgg actccggttc cgtagcggtg gaagtggcga tgaaaatggc
     5701 gttgcagtac tggcaagcca aaggcgaagc gcgccagcgt tttctgacct tccgcaatgg
     5761 ttatcatggc gatacctttg gcacgatgtc ggtgtgcgat ccggacaact caatgcacag
     5821 cctgtggaaa ggctatctgc cagaaaactt gtttgctccc gccccgcaaa gccgcatgga
     5881 tggtgaatgg gatgaacgcg atatggttgg ttttgcccgc ctgatggcgg cgcatcgtca
     5941 tgaaatcgca gcggtgatca ttgagccgat tgtccagggc gcaggcggga tgcgcatgta
     6001 ccatccggaa tggttaaaac gaatccgcaa aatgtgcgat cgcgaaggta tcttgctgat
     6061 tgccgacgag atcgccaccg gatttggtcg taccggcaaa ctgtttgcct gtgaatatgc
     6121 agaaatcgcg ccggacattt tgtgcctcgg taaagcctta accggcggca caatgaccct
     6181 ttccgccaca cttaccacgc gcgaggttgc agaaaccatc agtaacggcg aagccggctg
     6241 ctttatgcat gggccaactt ttatgggcaa tccgctggcc tgcgcggcag caaacgccag
     6301 cctggcgatt atcgaatccg gcgaatggca gcagcaggtg gcggctattg aagtgcagct
     6361 gcgcgagcaa ctggcaccag cccgtgatgc cgaaatggtt gccgatgtgc gcgtactggg
     6421 ggcaatcggt gtggtcgaaa ccactcgtcc ggtgaatatg gcggcgctgc aaaaattctt
     6481 tgtcgaacag ggggtatgga tccggccttt tggcaaactg atttacctga tgccgcccta
     6541 tattattctc ccgcaacagt tgcagcgtct gaccgcagcg gttaaccgcg cggtacagga
     6601 tgaaacattt ttttgccaat aacgggaagt cagcgtgagg gtttctggct acactttctg
     6661 caaacaagaa aggagggttc atgaaactca tcagtaacga tctgcgcgat ggcgataagt
     6721 tgccgcatcg tcatgtcttt aacggcatgg gttacgatgg cgataatatt tcaccgcatc
     6781 tggcgtggga tgatgttcct gcgggaacga aaagttttgt tgtcacctgc tacgacccgg
     6841 atgcgccaac cggctccggc tggtggcact gggtagttgt taatttaccc gctgataccc
     6901 gcgtattacc gcaagggttt ggctctggtc tggtagcaat gccagacggc gttttgcaga
     6961 cgcgtaccga ttttggtaaa accggatacg atggcgcagc gccgccgaaa ggcgaaaccc
     7021 atcgctacat ttttaccgtt cacgcgctgg atgtagagcg tattgatgtc gatgaaggag
     7081 ccagcggcgc gatggtcggg tttaacgttc atttccactc tctggcaagc gcatcgatta
     7141 ccgcgatgtt tagttaatca ctctgccaga tggcgcaatg ccatctggta tcacttaaag
     7201 gtattaaaaa caactttttg tctttttacc ttcccgtttc gctcaagtta gtataaaaaa
     7261 gctgaacgag aaacagtaaa aatcattaat atcaatgcat tataatacat ttagcttaaa
     7321 aaatagactg cataatactg caaaacacaa catatccagt cactatgaat caaccactta
     7381 gatggtatta gtgacctgat acagagcatt agcgcaaggt gattttttgt cctcttgcgc
     7441 taattttttg tcaacgcact gggttagctg aatttacaac catgctcagt atctcgataa
     7501 gcgcagagaa atgatgc
//
LOCUS       DADLSE010000169         7506 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLSE010000169 DADLSE010000000
VERSION     DADLSE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.170"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1183
                     /gene="pgaB"
                     /locus_tag="JN692_003572"
                     /pseudo
     CDS             1..1183
                     /gene="pgaB"
                     /locus_tag="JN692_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309296.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
     gene            1176..2516
                     /gene="pgaC"
                     /locus_tag="JN692_003573"
     CDS             1176..2516
                     /gene="pgaC"
                     /locus_tag="JN692_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HAY7265774.1"
                     /translation="MINRIVSYRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPF
                     FMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAA
                     LAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQKQGKAIALKTGAAAAK
                     SEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSI
                     IGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIF
                     YEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLT
                     TIWAFTCLAGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRY
                     EHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            2518..2931
                     /gene="pgaD"
                     /locus_tag="JN692_003574"
     CDS             2518..2931
                     /gene="pgaD"
                     /locus_tag="JN692_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HAY7265775.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN692_003575"
     CDS             complement(2981..3904)
                     /gene="phoH"
                     /locus_tag="JN692_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HAY7265776.1"
                     /translation="MRSRFSAFYPKANYGLQGSQPSDVRVHNRAANGACDEYKQLKVL
                     SMGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPI
                     LARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQ
                     ADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVETAPFAYMRGR
                     TFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVCSGLSDALER
                     FEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN692_003576"
     CDS             complement(4377..5642)
                     /gene="efeB"
                     /locus_tag="JN692_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HAY7265777.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(5648..6775)
                     /locus_tag="JN692_003577"
     CDS             complement(5648..6775)
                     /locus_tag="JN692_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HAY7265778.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(6833..>7506)
                     /locus_tag="JN692_003578"
     CDS             complement(6833..>7506)
                     /locus_tag="JN692_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HAY7265779.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1882 a         1921 c         1773 g         1930 t
ORIGIN      
        1 aaatgcgtca caattggatt ccattccgcg agtattaatc gccaataatc cctcattaaa
       61 agagtttgcc cagcaaatta ttaccgtaca ggaaaaatca ccacaacgga taatgcatat
      121 cgatcttgat tacgtttatg acgaaaaccg ccagcaaatg gatcgcaata ttgatgtgct
      181 aattcagcgg gtgaaagata tgcaaatatc aaccgtgtat ttgcaggcat ttgctgatcc
      241 cgatggtgat gggctggtca aagaggtctg gtttccaaat cgtttgctac caatgaaagc
      301 agatattttt agtcgggttg cctggcaatt acgtacccgc tcaggtgtaa acatctatgc
      361 gtggatgccg gtattaagct gggatttaga tcccacatta acgcgagtaa aatacttacc
      421 aacaggggag aaaaaagcac aaattcatcc tgaacaatat caccgtctct ctcctttcga
      481 tgacagagtc agagcacaag ttggcatgtt atatgaagat cttgccggac atgctgcttt
      541 tgatggcata ttgttccacg atgatgcttt gctttcagat tatgaagatg ccagtgcacc
      601 ggctatcacg gcttatcagc aagcaggctt tagcgggagt ctgagcgaaa ttcgataaaa
      661 cccggagcaa tttaaacagt gggcccgctt taaaagtcgt gcgttaactg acttcacttt
      721 agaacttagt gcgcgcgtaa aagccattcg cggtccacat attaaaactg cacgaaatat
      781 ttttgcactt ccggtaatac aacctgaaag tgaagcctgg tttgcacaga attatgctga
      841 tttcctaaaa agctatgact ggaccgctat tatggctatg ccttatatgg aaggtgtcgc
      901 agaaaaatcg gctgaccaat ggttaataca attgaccaat caaattaaaa acatccctca
      961 ggctaaagac aaatctattt tagaattaca ggcacaaaac tggcagaaaa atggtcagca
     1021 tcaggctatt tcttcgcaac aactcgctca ctggatgagc ctattacaac tgaatggagt
     1081 gaaaaactat ggttattatc ccgacaattt tctgcataac caacctgaaa tagaccttat
     1141 tcgtcctgag ttttcaacag cctggtatcc gaaaaatgat taatcgcatc gtatcgtatc
     1201 gtatcgtatc gttttttata ttatgtctgg tgttatgcat acccctatgc gtagcgtact
     1261 ttcactctgg tgaactgatg atgaggttcg ttttcttctg gccgtttttt atgtccatta
     1321 tgtggattgt tggcggcgtc tatttctggg tctatcgtga acgccactgg ccgtggggag
     1381 aaaacgcacc agctccccag ttgaaagata atccgtctat ctccattatc attccctgtt
     1441 ttaatgagga gaaaaacgtt gaggaaacca tacacgccgc tttagcacag cgttatgaga
     1501 acattgaagt tattgccgta aatgacggtt caacagataa aacccgtgcc atcctggatc
     1561 gcatggctgc acaaattccc catttgcggg tcattcatct ggcgcaaaaa caggggaaag
     1621 ccattgcgct taaaaccgga gctgccgcgg cgaaaagtga atatctggtg tgcattgatg
     1681 gcgatgcgtt attagaccgc gatgcggcgg catatattgt ggaaccgatg ttgtacaacc
     1741 cgcgtgtggg tgccgtaacc ggtaatcctc gtattcgaac acgttctacc ctggtgggta
     1801 aaattcaggt tggcgagtat tcctcaatta ttggtttgat caagcgaacc cagcgtatct
     1861 atggaaacgt atttaccgtt tccggtgtta ttgccgcatt tcgtcgcagc gccctggcag
     1921 aagtgggtta ctggagtgac gatatgatca ccgaagatat tgatattagc tggaagctgc
     1981 agttgaatca gtggacgatt ttttacgagc cacgggcgct gtgctggata ttaatgcctg
     2041 aaacgttaaa agggctgtgg aaacagcgcc tgcgctgggc tcagggcggt gcagaagtat
     2101 tcctcaaaaa tatgacaagg ttgtggcgca aagaaaactt tcgaatgtgg ccgctgtttt
     2161 ttgaatactg cctgacgaca atatgggcct tcacctgcct ggccggtttc attatttacg
     2221 cagtccaact tgccggtgta ccgttaaata ttgaattgac acatatcgct gcgacacata
     2281 ctgccggaat attattgtgt acgttatgtt tactgcaatt tattgtcagc ctgatgatcg
     2341 agaatcgcta tgagcataat ctgacttcat cgcttttctg gattatttgg ttcccggtta
     2401 ttttctggat gctgagcctg gcaacgacat tggtatcatt tacacgagtc atgttgatgc
     2461 ctaaaaagca acgcgcccgt tgggtaagtc ctgatcgcgg gattctgaga ggttaatatg
     2521 aacaatttaa ttattacgac ccgacaatca ccagtacgtt tactggttga ttatgttgcc
     2581 acaaccatct tgtggacatt atttgctttg ttcatattct tattcgccat ggatctgctg
     2641 acgggttatt actggcaaag cgaggccaga agccgacttc agttctattt tttgctggca
     2701 gtggcgaatg ccgtcgtgtt aattgtctgg gcgctgtaca ataagctgcg ttttcaaaaa
     2761 cagcagcatc atgcagccta ccaatatacg ccgcaagaat atgcagagag cttagcaata
     2821 cctgatgagc tctatcagca actacaaaaa agccacagga tgagcgtaca cttcaccagc
     2881 caggggcaaa taaaaatggt tgtttcagaa aaagcgctag tccgggcata aacacccaaa
     2941 acaaagcccg gttcgcccgg gctctgcacc gataatacac ttaactgtat gcatgcagcg
     3001 tacgttggca aagtgccgaa cgtacgcaat cctcttttcc gaaccggacg atcccaacca
     3061 tttcatcttc ttcgaaacgt tccagcgcgt cacttaatcc ggagcacacg ccgcgaggca
     3121 aatcgcattg cgtgatatca ccgttgacga taaccgtcac gttctccccg aggcgggtta
     3181 aaaacatttt catttgcgcg gcagtcacat tctgcgcctc gtcaagaatg acgactgcat
     3241 tttcaaaggt acgtccacgc atataggcga acggcgcggt ttccaccttc cctatttccg
     3301 gtcgcaggca gtactgcata aaggaagccc ctaagcgccg gaccagcacg tcgtagaccg
     3361 ggcgaaaata gggagcaaac ttttctgcga tatcaccagg taagaagcca agatcttcat
     3421 cggcttgcag aactggacgg gtgacgataa tcctgtcgac atccttatgt atcagggcct
     3481 ctgccgcttt tgctgcgctg atccaggttt ttccgcaccc ggcttcgccc gtggcgaata
     3541 tcagctgctt actctcaata gcctgcagat agtgcaattg cgcttcattt cgcgcaagga
     3601 tgggcgaggt atcgcgactg tcgcgggcca taccaatggc ttctacgccg ctcatctgca
     3661 caagcgaggt gaccgattct tcttcacgct gcttatggct gcgcgaatcc cgtctcagca
     3721 cacgttttgc ctcgcgacga gctttgatca ctgctttttg tcttcccatg gagagcacct
     3781 tgagttgttt gtattcatca cacgcgccgt tggcagcgcg attatgcaca cgaacatcag
     3841 agggttggct tccttgtaag ccatagtttg cttttggata aaatgccgaa aaacggctac
     3901 gcacaccgtt tgcggcgtcg gtaacacgag taaagaaagg aagtgagaaa aatacagagt
     3961 gacgaagttg ctaccggagg agaaatctcc gcgagtggtg gaacgttgct taacaaaaca
     4021 ttgtccagaa caggacgtta ccatccgcga tctccatagt gactgactat cactgccggg
     4081 aacttccgct gctacttaat aagtacaaca gaactcgcat tcattgcaac aatatattca
     4141 cttatattta acgataaata cccatttcgg tgacattaat ttctactgga aagatgacag
     4201 agtgatgaca gtgatggaaa aagctgtgtg ctttccgcag gattcgcagg gctggtaaaa
     4261 tgtgcctcgt gtttgccgga ttcggcgcga acgccttacc cgacctacaa gtgattaata
     4321 atttgtgcac aaaactcatg cccgataaac ctgcgcatcg ggcatatttg cgtcgttcaa
     4381 acctgcaata gcgcccttcc cagataatcg ttcgcgtcct tcactcccgg cagcgaaaaa
     4441 aaataaccgc cgccgatagg tttaacgtat tcctccagcg cttcgccatt gagccttttt
     4501 tgtactgtca ggaagccttt ttccagatcg tgttggtagc agacaaacag caaccccata
     4561 tccagttgtc cggagttggt gacccccaac gaataactgt agccgcgacg cagcatcagg
     4621 ctggactcac tctccgccgt gcggggattc gccagccgga tatggctgtc cagcgcgatc
     4681 accttccctt ccgggtcgct ggcgtaatca ggcacatcat gctcatgctg cattcccagc
     4741 ggcgcaccgg tttgcttatc acggccaaaa atcgtctgct gttctttcag cggcgttctg
     4801 tcccaaaatt ccactcgaaa ctgaatcaag cgtactgcct gatagctgcc accgattgtc
     4861 cacgcaggct cctgctgatc tgccgttacc cacaccactt tttgcatcaa cttatcattc
     4921 tggctatcgg gattggcagt gccgtctttg aaacccagca aattaatcgg cgtctctttg
     4981 cctttactac gcgccgcgtg atcggaaata aacccttccc gcttccagcg cacactgagc
     5041 aaatccggcg tgtgtttgat gatatcgcgc agcgcatgga tcaccgtgtc ctgggtgttg
     5101 gcgcaaatct gtagcaacac atcaccatga cataacgccg catccagcga gtcgttgggg
     5161 aaacgcgtca tcttctgcag cttttttggc atctgtggcg caaggccaaa gcgctcatca
     5221 aacaatgagt gacccaccga taacgtgatg gtgagattat cgggcgcaat gtagccgcca
     5281 agaatgccgg aatcgagtgg tggcaggcgc ggattcggcg tttctggtgc tgctccgccc
     5341 tgactcagaa aagcaaaacg ctgagtcaac aagcgaaaca accgctcaag atcggcttta
     5401 tcgctggcaa gcacatcaaa cgccaccagc atcattgcgg cctgttgtgg cgtcaggatc
     5461 cctgcctgat gctcaccata aaacggctgt ttctcattgc gtgcatccgg tgaaagcgtg
     5521 cccggtgcac tttgcgtttt ttgtgcatga gcgaccggac aacttcccgc cagcgcacca
     5581 atccctttca gtaaacgtcg gcgtgacggt tcattcacgc cgttttcatc tttatactgc
     5641 ataacgctta atccagtccc agcacaccgc gaagttgcgc cagatcttcc gccagcgcag
     5701 taatcggccc tttcagcgca ttccggtcgg catcggtcaa tttgtcgtag gtttcaaaac
     5761 cgtctttagt acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt
     5821 ttgccaacag ttccgggttg gctttttgca gttgtggacg cagcagatcg acaattttct
     5881 gcgagccttc aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt
     5941 caccgctgat tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt
     6001 ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa
     6061 gctgctcagc gtactgatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca
     6121 gacggtggaa accagtgaat tttgggtcgg cggctttttg ctcgtaatca tcttcacggg
     6181 cgtcaatgct gccatccaga tcggagaaca gttcagcaat cggttcaata cgctcatagt
     6241 gctggcgcgt cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt
     6301 cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc cgccatgaca tacgctttat
     6361 atgcagtaat tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg
     6421 cctcaccttt gacgatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcgt
     6481 attcgccagg ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc
     6541 gctcttccac caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt
     6601 tctgaataat gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgct
     6661 tatccgtcac ggtcactttg acctgcggca catcagcggc gttagccata aaagcagaag
     6721 aaaacagcgc agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga
     6781 gactatccct ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgttacgcgg
     6841 agcgagacgc tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccgggatga
     6901 gataaataaa ccagacggcg acttcgctga cgctcggtgc ttcctgatag ccaaaaatcc
     6961 cttccatcag cgtgccaaac agcgagtgag ttgagaggac cgcgctcata tcgaaggcga
     7021 tttcctgaaa gtggttccac aatccggctt catgaaatgc gcgaatggca ccagccgcca
     7081 gccctgcggc gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga
     7141 ggcgaatacc gccccagtag agcaggaagc cgagcaccac ggcagtagca agaccgagca
     7201 ttgcacccag cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga
     7261 ccgactccag cccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat
     7321 gatgatttcc acgctgcaat gcgctatcga ctgcctgttc cagttgcact ttgacgttgc
     7381 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     7441 cgataccttc aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga
     7501 agatcc
//
LOCUS       DADLSE010000170         7494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLSE010000170 DADLSE010000000
VERSION     DADLSE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.171"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..544
                     /locus_tag="JN692_003579"
     CDS             <1..544
                     /locus_tag="JN692_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HAY7265780.1"
                     /translation="RYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVLDQPTM
                     KALDKQNVAQALSVVPGVVLQKSGSRNEKQVKVRGFDSRQVPVYFDGVPIYVPYDGNL
                     DLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVRTMPTI
                     CMLHLPPAAILGICKSAVAS"
     gene            481..1941
                     /locus_tag="JN692_003580"
     CDS             481..1941
                     /locus_tag="JN692_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7265781.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKE
                     SFYYQGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYFDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKEGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(1991..2245)
                     /locus_tag="JN692_003581"
     CDS             complement(1991..2245)
                     /locus_tag="JN692_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7265782.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            2446..3180
                     /locus_tag="JN692_003582"
     CDS             2446..3180
                     /locus_tag="JN692_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7265783.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYNLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN692_003583"
     CDS             complement(3182..3793)
                     /gene="emtA"
                     /locus_tag="JN692_003583"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7265784.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            3893..4807
                     /gene="ldcA"
                     /locus_tag="JN692_003584"
     CDS             3893..4807
                     /gene="ldcA"
                     /locus_tag="JN692_003584"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7265785.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            4902..6638
                     /locus_tag="JN692_003585"
     CDS             4902..6638
                     /locus_tag="JN692_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7265786.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            7249..>7494
                     /locus_tag="JN692_003586"
     CDS             7249..>7494
                     /locus_tag="JN692_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265787.1"
                     /translation="MASFKDICLFAPAIPPSSVNPGVRRPPGRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSIAGKVNIQVDNNTIITL"
BASE COUNT         1820 a         1910 c         1905 g         1859 t
ORIGIN      
        1 acgttatctc tgcacagcat tatcacttgc ctttacccag caggccgtag cggctcagga
       61 gagcgacacg ctgaccgtat ggtccagtcc ggtatcatcg acgacgacca ccgttctcga
      121 tcaacccacc atgaaggcct tggataaaca gaatgtcgct caggcattaa gtgtcgtccc
      181 cggcgtggtg ctgcaaaagt caggcagccg caacgaaaaa caggtaaaag ttcgtggctt
      241 tgatagtcgg caggtgccgg tctatttcga cggtgtgccc atttatgttc cctatgacgg
      301 caacctcgat ctggcgcgga ttctgaccaa caatctgggg gcagtcgaag tttccaaagg
      361 gtattcgtcg ctgcttcatg ggcctaatca gatgggcgga gccattaata tcaccaccct
      421 gaagaagcaa gtctgggata tcgccaggga tggagccgta gtcaggacaa tgcctacgat
      481 atgcatgctt catttgccgc cagcagcgat cttgggtatt tgcaagtcag cggtagccag
      541 ctaaagcagg attttctcgg cctgccgcat ggtgtaaata atgatattgc aggcaaacac
      601 ggcaagatga ttaattcatc ggctgatgat aaacgcggca ttgtgaagct cggttttaca
      661 ccgcgcgaaa acgatgaata cacgctgact tacattaagc aggatggtga aaaagataac
      721 ccgccataca gcggaaatag tggtcaaaaa tcacgctact ggcagtggcc agagtatgac
      781 aaagaaagtt tttattatca gggaacgacc caactaaacg accgttttac cctgaaaagt
      841 cggctgtatc gcgacacctt tgaaaatacg ctgatgatgt acaactcgct ggctgatttg
      901 aaaaataaaa aaggcagcta cagccattat ttcgattaca gcgacggtgc cggattacaa
      961 ctggcagccg atgtgcgtga aaacgatctg ctgtcgtttg ccgttaactg gaaagatgac
     1021 gtacaccggg aaaaaggtgc gccgcacgcc gcttacgatc gctatgaaga tcgtacctgg
     1081 tcgctcgcca gtgaatatca atgggctgct gctgataatg tcgatgtcgt ggcgggaatc
     1141 agctatgact ggcgcgatag cgtagaagcg aaaaaacacg agaaagaggg cagcatcacc
     1201 cattatgacg acaacaatca gtcagctttt aactggcagg tgatggggaa ataccacttt
     1261 gccaatgaag acactctggc gctttcgtac tatgaccgca cacgctttcc gacgctgaaa
     1321 gaacgctata ccacatccaa acctgcgtat aaccagatag cgattgttaa cccgcagctc
     1381 aagccggaac gcgctcgcgg ggtggattta acctggaatg gtgccttcac gcgcgactgg
     1441 ggatttgagg tcagcgttta ctataaccgg gtgagtgatg ccatcctctc gcacaatatc
     1501 aatgccgata ccattcaaaa tcagaacagc ggcacggtgg attacagcgg tctggatgcc
     1561 ggtattaagg ggaaaatcag caatatactg gatgtaggat tgagctacgc tctgatccac
     1621 gctgacgcca aacgcaaaga catcggcaag ataaccgatc ttccaacgca gacaatgacc
     1681 gcatggatga cactcaaacc atgggagccg ttaagcgtaa cgctgtcgga agaggcgcgt
     1741 tcctccagct acagcaacag tgacggttca caaaaagccg ccggttttgc ggtgacccac
     1801 attcgagccg attacacctt aggtcatggc ttcagcgtca atgcgtcggt caataacctg
     1861 tttgataccc aatatgccta cagtgaaggg tttattgaag aaggtcgcaa tttctgggca
     1921 ggtgtcgagt atacgttctg atgtgaaagc ggatagcggc atctgtatcg ctatccgcct
     1981 tatgaaaatc ttattctctt cgcaggaggc ggaatacgcc cagcaccagg atagcgccca
     2041 ccaccgccac caggaagctg tgcaaattaa aaccgctgat ggagccgcca atgccaaaca
     2101 tggtcgccag ccagccgccg accaccgcac cgactatccc gagaatacag gtcaggaaaa
     2161 atccaccacc atcacgcccc ggcatgatta gcttggcgat aatgccggct atcaggccaa
     2221 aaataatcca ggcaataatt cccatgttca ggccctcgtt tcgatgaatc ggtcgcaaga
     2281 aatgcttacg tcaacgctct aagtataggc aagggcggga tcgatgctgg gcgtttgtcg
     2341 gctgatttaa gaacaacatg taaaaaatat tcacgcattt gtattaagtt ttgttaacta
     2401 tgaccgataa accaaagaca gtttgtcagt caggagtttt tccgcgtgag tcattaccat
     2461 gagcagttcc tgaaacaaaa tccgttagcc gtcctgggcg tgttacgcga tttgcacaaa
     2521 gccgcaattc ctttgcgtct cagttggaat ggcgggcagc tgatcagcaa aatactggca
     2581 ataaccccgg ataaactggt gctggatttc ggcagtcaag ccgaagacaa catcgccgtg
     2641 ctaaaggcac agcacattac cattaccgcc gaaactcagg gtgcgaaagt cgagtttact
     2701 gttgaacaac tacagcagag tgaatacttg cagcttccgg catttattac cgtaccgcct
     2761 cccaccttat ggtttgtaca acgacgccga tatttccgca tctccgcccc actccatccg
     2821 ccttattttt gccagaccaa actggcggat aacagtacgt tacgtttccg cttgtataat
     2881 ttgtcgttag gcggcatggg cgcattactg gaaacagcaa agcctgccga attacaagaa
     2941 ggtatgcgct tcgctcaaat tgaagtcaac atggggcaat ggggtgtttt tcactttgac
     3001 gcccagttaa tctccaccag cgagcgcaaa gtgattgatg gcaagaatga aaccatcacc
     3061 actccccgtc tgagcttccg ttttcttaac gtcagcccga cggtggagcg gcaattacag
     3121 cggattattt tctctctcga gcgagaagcc cgggaaaaag cggacaaagt gcgcgactga
     3181 tttacatcgc gtccagtgcc tgctcaagtt tgtagatata gcgcggagcc tgcggcgcag
     3241 gatgatttcg cgctacgtga tcgaggaact cgtcagcatc caaatcgttg attttgctga
     3301 tcgccttttt ccgatctgac gagaaagtcc gtagcagcgc acctgccccg ttagcgtatg
     3361 acaccaccag cgcatattgc agtaccttcg gatcttcaat gcctgccagc gggccggttt
     3421 ccagaatatt taggtaagcc gcccccattg aaatattacg ctctggattt ttcagctcgc
     3481 tggtcgtcgg ctcaccactc cagcccatac ggcgataaac atcacgtccg gaggttgaag
     3541 cttttaactg catcaaacca atggcattcg atttactcac cgcgttcgga ttaccacccg
     3601 attcgatagc gataatcgcc gtgatcaatt gtggatcgac gccccaggct gcaccggctt
     3661 tctggcttat tggcatccac tgcatcgcac gttgcaccgg aactttcgcg ttccacggcg
     3721 ggttcgtata gtcatgcttt gatgaacaac ccgctaataa cacaatcaaa aaggcaaacc
     3781 atctcaattt cacttcttct atccttatag cctggaatcg ccgttgaggt gacaacggca
     3841 cacctaagcg gcatgatatg catttcgttt ctgttgtcag caaggaattg ccatgtctct
     3901 gtttcactta attgccccat cgggttacag cattaaacag cacgccgcgc tgcgtggtat
     3961 ccagcgcctt accgacgcgg ggcatcaagt caataacgtc gaggttattg cccgtcgttg
     4021 tgagcgtttt gcgggtacgg aaacggagcg tctggaagat ctcaattccc ttgccagact
     4081 gactaccccc aacaccatcg tgctggctgt acgcggcggt tacggtgcca gtcgtttact
     4141 ggcagatatt gactggcagg cactggtggc tcgccaacaa catgacccgt tactcatttg
     4201 cggacatagc gattttaccg ccattcagtg cggtcttctg gcgcaaggca atgtcatcac
     4261 ctttagcggc cccatgctgg tagcaaactt tggtgcggat gaactgaacg cctttaccga
     4321 gcaccatttc tggctggcgt tacgcaatga aaccttcacc attgaatggc aaggggaagg
     4381 cccgacatgt cagaccgaag gcacgttgtg gggaggcaat cttgcgatgc tgatttcact
     4441 gattggtaca ccgtggatgc caaaaattga gaacggtatt ctggtgctgg aagatattaa
     4501 cgagcaccct ttccgcgtcg aacgtatgct gttgcagctc tatcatgcgg gaattttgcc
     4561 acgtcagaag gcgattattc tcggtagctt tagcggcagc acgcccaatg attatgacgc
     4621 aggttacaac ctggagtcag tgtacgcgtt tttgcgttcc cgcctgtcga ttccactcat
     4681 taccggcctc gattttggtc atgaacaacg cacggttacg ctgccgttgg gcgcacatgc
     4741 cattctgaat aacacccggg aaggcactca gttgacgatt agcggtcatc ctgttcttaa
     4801 aatgtaaaaa aatcacgctt cagggctaag taaaacgctg tctctgccgc taatatggta
     4861 gggtttatat aagagacagc gtaatcagga gtaaccgacc gttggatgcc acaacaataa
     4921 ttagcctttt catcttagga tccatccttg tcaccagcag tatattactt agttcatttt
     4981 cttcccgtct tggcattcct attctggtta tttttctggc gatcggcatg ctggcgggag
     5041 tcgatggcgt cggcggtatc ccgtttgata attacccctt cgcctacatg gtaagtaacc
     5101 tggcgctggc gattattttg ctcgacggcg ggatgcgcac tcaggccagt tcctttcgtg
     5161 tggcgttagg accggcgctg tcgctggcga cgctgggcgt acttatcacc tctggtttaa
     5221 ccggcatgat ggcggcgtgg ctgtttaatc ttgatttgat tgaaggctta ttaatcggcg
     5281 ctatcgtcgg ctccaccgat gctgcagcgg tcttttctct gctgggtggt aaggggctta
     5341 acgaacgtgt tggctcgacg ctggaaattg aatccggcag taatgatcca atggcggtct
     5401 ttctgacgat taccctgatt gcaatgatcc agcagcatga aagcagtgtc agttggatgt
     5461 tcgtggtcga tattctgcag caatttggcc tcgggattgt catcggtctt ggcggtggtt
     5521 atttactgct gcaaatgatt aatcgcatcg ccctgcctgc cggattatat ccattgctgg
     5581 cattaagcgg cggtatttta atttttgctt taaccacggc gctggaaggc agcggtattc
     5641 tggctgttta tctgtgcggt tttctgctgg gtaatcggcc gattcgcaac cgctacggca
     5701 tcctgcaaaa tttcgacggc ctcgcctggc tggcgcaaat cgccatgttc ctggtgctgg
     5761 ggctattggt taacccaagc gatctgctgc ctattgccat tccggcgctc attttgtcgg
     5821 catggatgat attcttcgcc cgtccccttt cggtatttgc tggattgctc cctttccgcg
     5881 gtttcaatct gcgtgagcgc gtgtttatca gctgggtggg attacgcggc gcggtgccaa
     5941 tcatcctggc cgtgttcccg atgatggcgg ggctggagaa tgcgcgactg ttctttaatg
     6001 tcgccttctt tgtggttctg gtttcactgc tcttgcaggg aacatcactc tcgtgggcgg
     6061 cgaaaaaagc caaagtggtg gttccgcccg tcggacgtcc ggtgtcacgc gttggcctgg
     6121 atattcatcc ggaaaatccg tgggagcagt ttgtttatca attgagtgct gataaatggt
     6181 gtgtaggtgc ggcactgcgc gatttgcata tgccaaaaga gacgcgtatt gcagccctgt
     6241 ttcgtgataa ccagttgctt catcctaccg gcagcacccg actgcgcgaa ggcgatgtat
     6301 tgtgtgtaat tggtcgggaa cgcgatctcc cggcgctcgg taaactgttc agccagtcgc
     6361 caccggtcgc gctggatcaa cgcttctttg gtgacttcat tctcgaagcc agcgccaaat
     6421 atgctgatgt tgcgctgata tatggcctgg aagacgggcg agaatatcgt gataaacagc
     6481 aaacgctggg tgaaattgtc cagcagttgc taggcgcggc accggttgtc ggtgaccagg
     6541 tagagtttgc cgggatgatc tggacggtgg ccgagaaaga agataatgaa gtgttgaaga
     6601 ttggcgttcg ggtggctgaa gaggaagctg aatcttaaat ttaatgcagc atctatcgcc
     6661 ggatgcgatg ctttactgaa cgcagcctga gcctgtaata aacttgtcat ttcctgattt
     6721 tgatatgctc atcccaacat cagaagaagg ttaaattatg gatgacaaac cattgcaggc
     6781 tctggctaat gaactcaaaa caccggctca agaaagtcag cgtaagggct ttttccgact
     6841 gtaacaggta ataaaaacca gccccgcatt accggtcaac catcaggggc ccctgcctga
     6901 catcgcctga acatattccc ccacgcaacc tgccgcactg acatagccca cacaataggc
     6961 gtttcatccg cctgtagcac cggatgcatg agcgatttct tatagttatt cctttgctta
     7021 ataacgatta tttccgggtc gttcactctc gtattttctt taatacgtca gagctaccta
     7081 ggccgtaata cagcatgtac cagacccgac acggcatttc tgaccaggga aaaagtcaac
     7141 gatgagtagt gatatcagcg caaatgacgt cctgtggctc ctttgcggtg accacattca
     7201 cgtctccggc gctgccagag gatatttatt tcaaggaaac gtagcattat ggctagcttt
     7261 aaagacatct gcctattcgc ccccgccatt cccccatctt ccgttaaccc aggcgtacga
     7321 cgcccgccag ggcggctctt cctgtcggtg gaactcaaga atcaaatttt gcagaacatg
     7381 aagtatctca gcccgcaaaa attcagctgg ggtgatgccc cctggcagat cattgacctc
     7441 agtatcgcgg gcaaagtgaa catacaggtg gacaacaata ccatcatcac gttg
//
LOCUS       DADLSE010000171         7491 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLSE010000171 DADLSE010000000
VERSION     DADLSE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.172"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(76..357)
                     /locus_tag="JN692_003587"
     CDS             complement(76..357)
                     /locus_tag="JN692_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7265788.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN692_003588"
     CDS             complement(619..1728)
                     /gene="apbC"
                     /locus_tag="JN692_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7265789.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1860..3893
                     /gene="metG"
                     /locus_tag="JN692_003589"
     CDS             1860..3893
                     /gene="metG"
                     /locus_tag="JN692_003589"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7265790.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERTEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4034..7491
                     /locus_tag="JN692_003590"
                     /pseudo
     CDS             4034..7491
                     /locus_tag="JN692_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
BASE COUNT         1944 a         1946 c         1808 g         1793 t
ORIGIN      
        1 tatcaataaa aaagacgaaa tcatttatat aaatgatttc gtctttctaa atgcaattaa
       61 aatcaggttg ccaaattaat cgtagcgatt taacacgatt cgacgtttgc tatcgctacc
      121 tgcaacaacc acttctgtcg taacaccttt gacgggcgaa gaaacctgat gacgggtgtc
      181 aacattatac gttaactgac cacaatgctg ttgtggttga ctattaacca tagccatact
      241 gcatgttggg ggactgatcc tgccattaat cgttaatgtg gcagtctctg aagagactgc
      301 gggagaagcc aatccatagg caagaaaaat aattcctgac agccaatact tattcattga
      361 acgttatccc tgtagtaaag gttatgcctg gatagaatga gtgcataaca aactatagct
      421 gtacatccac tacacagcac gaaggatgat aatgaagcat tgcctgtacg atcactcgac
      481 tttgtagagt ttcggacgaa ggtccgcaga atattcgcag tattaaataa gtgttcatta
      541 atgcggcaaa aacaaataca ccgcatcaat ataacatctc tgcggtgtat gaagaataac
      601 cagaatggtt aataggcgtt agaccgcgcg gaaggaaatc tcgcctggaa tgacttcacc
      661 ctgccagtag agctgagctg caacgcggtc agccagctgg cggtagatag cggtaaattc
      721 gctctccgga cggctgataa ccgttggcgt gcctttatcg agatcctcac gtaaggagat
      781 gtgtaacggc atctgcccca gcagctgtgt atggtatttt tcagccagtt tctctgcacc
      841 accggtaccg aaaatcggtt catggtgacc acagttactg caaatatgca cactcatgtt
      901 ctcgacaata ccgagcaccg gaacctcgac tttctcgaac atcacaatgc cttttttcgc
      961 atcgatcaac gcgatgtcct gcggggtggt gaccacgact gcaccagtta ctggaatgtt
     1021 ttgcgccagc gtcaattgaa tgtcaccggt gcctggcggc atatcaagca ccagatagtc
     1081 gagatccggc cacaaggttt cctgcaacat ctgcattaac gccttgctcg ccattggtcc
     1141 acgccacacc atagcattgt cgtcagtgac cagataacca attgaattgg ttgccaggcc
     1201 atgagacata atcggtgcca tgtgggtacc gtccggcgaa gttggacgct gattttctgc
     1261 gcccagcatg gtgggaattg atgggccgta gatatcggcg tccagaatgc cgactttcgc
     1321 cccttccgcc gccagtgcca gcgccaggtt taccgccgtg gacgatttac ccacgccgcc
     1381 tttaccggaa ctcaccgcga taatgttctt cacaccgtta attcccggct gattcttcac
     1441 gcgtttcaga gtggctatgt tatgcgacag tttccagtcg atagctttcg ccccggtgat
     1501 gcgcagcagt tcggcgctac attgctcttt taactcttcg aacgcgctat gccagacaaa
     1561 cggcataacc agttccacat gcaacgtgtc gtccatccag gcgacatggt gcaacgcttt
     1621 cagcgtggtg aggttatgct tcagggttgg gtgctgaaaa ttggccagtg tcccggcgac
     1681 cattgcgcgc aaggcttctg gtgatttggc ctgggattgt tcgttcatcc cgactccttt
     1741 tttgtataga taaaccatca gctgatagtt tacctgaaga atatagagaa gtacttactt
     1801 aacattttcc catttggtac tatctaaccc cttttcacta ttaagaagta atgcctacta
     1861 tgactcaagt cgcgaagaaa attctggtga cgtgcgcact gccgtacgct aacggctcaa
     1921 tccacctcgg ccatatgctg gagcacatcc aggctgatgt ctgggtccgt taccagcgaa
     1981 tgcgcggcca cgaggtcaac ttcatctgcg ccgacgatgc ccacggtaca ccgatcatgc
     2041 tgaaagctca gcagctcggt atcaccccgg agcagatgat tggcgaaatg agtcaggagc
     2101 atcagactga tttcgcaggc tttaacatca gctatgacaa ctatcactcg acgcacagcg
     2161 aagagaaccg ccagttgtca gaacttatct actctcgcct gaaagaaaac ggttttatta
     2221 aaaaccgcac catctctcag ctgtacgatc cggaaaaagg catgttcctg ccggaccgtt
     2281 ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca atacggcgat aactgcgaag
     2341 tctgcggcgc gacctacagc ccgactgaat tgatcgagcc gaaatcggtg gtttctggtg
     2401 ctacaccagt gatgcgtgat tctgaacact tcttctttga tctgccctct ttcagcgaaa
     2461 tgctgcaggc atggacccgc agcggtgcgt tgcaggagca ggtggcaaac aaaatgcagg
     2521 agtggtttga atccggcctg caacagtggg atatctcccg cgacgcgcca tactttggtt
     2581 ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg gctggacgca ccgattggct
     2641 acatgggttc tttcaagaat ctgtgcgaca agcgcggcga cagcgtaagc ttcgatgaat
     2701 actggaagaa agactccacc gccgagctgt accacttcat cggtaaagat atcgtttact
     2761 tccacagcct gttctggcct gccatgctgg aaggcagcaa cttccgcaag ccgaccaacc
     2821 tgtttgttca tggctatgtg acggtgaacg gcgcgaagat gtccaagtct cgcggcacct
     2881 ttattaaagc cagcacctgg ctgaatcatt ttgacgctga cagcctgcgt tactactaca
     2941 ctgcgaaact ctcttcgcgc attgatgata tcgatctcaa cctggaagat ttcgttcagc
     3001 gtgtgaatgc cgatatcgtg aacaaagtgg ttaacctggc ctcccgtaat gcgggcttta
     3061 tcaacaagcg ttttgacggt gtgctggcaa gcgagctggc tgaccctgag ctgtacaaaa
     3121 cctttaccga tgccgctgaa gtgattggtg aagcgtggga aagccgtgaa tttggtaaag
     3181 cagtgcgcga aatcatggca ctggctgacc tggctaaccg ctatgtcgac gagcaggctc
     3241 cgtgggtagt cgcaaaacag gaaggtcgcg atgctgacct gcaggcgatt tgctcaatgg
     3301 gcatcaacct gttccgcgtg ctgatgactt acctgaagcc ggtactgccg aaactgaccg
     3361 agcgtacaga agccttcctc aatacggaac tgacctggga tggtatccag caaccgctgc
     3421 tgggccacaa agtgaatccg ttcaaggcgc tgtataaccg catcgatatg aaacaggttg
     3481 aagctctggt ggaagcctct aaagaagaag tgaaagccac taccgcgcca gttactggcc
     3541 cactggcaga tgatccgatt caggaaacca tcacctttga cgacttcgcc aaagttgacc
     3601 tgcgcgtggc gctgatcgaa aacgcagagt ttgttgaagg ttctgacaaa ctgctgcgcc
     3661 tgacgctgga tctcggcggt gaaaaacgca atgtcttctc cggtattcgt tctgcttacc
     3721 cggatccgca ggcactgatt ggtcgtcaca ccattatggt ggctaacctg gcaccacgta
     3781 aaatgcgctt cggtatctct gaaggcatgg tgatggctgc cggtcctggc gggaaagata
     3841 ttttcctgct aagcccggat gccggtgcta aaccgggtca tcaggtgaaa taatccccct
     3901 tcaaggcgct gcatcgacag cgccttttct ttataaattc ctaaagttgt tttcttgcga
     3961 ttttgtctct ctctaacccg tataaatact ggtagcatct gcattcaact ggataaaatt
     4021 acagggatgc agaatgagac actttatcta tcaggacgaa aaatcacata aattctggac
     4081 ggtggagcaa cagggaaacg agttgcatat cagttgggga aaagttggca ccaaagggca
     4141 aagtcagata aaaagttttt cagatgctgc ggcggcggaa aaagcggaac tgaagctgat
     4201 tgcggagaag gtgaagaagg ggtatgtgga gcaagcgaaa gataattctt tgcaaccttc
     4261 ccaaacggta acggactctc tcaaggtagc ggacttatcc accattattc aggaacaacc
     4321 ctctttcgta gcagaaaccc gtgcggctga caaaaataca gatgctgttt taccttggct
     4381 ggcgaaagat attgctgtcg tttttccgcc cgaagttgta cacaccacgt taagtcatcg
     4441 ccgctttccc ggagttcctg ttcagcaagc agacaaattg acccaattac gtcgcttagc
     4501 ctgtagcgtg tcgcaacggg ataataaaac agccacattt gacttcagca cctgttcttt
     4561 agaatggcaa aacaccgtcg cccaagcgat cagtcagatc gacggcctga aaacaacaca
     4621 gctaccatca ccagtaatgg ctgtactcac ggcacttgaa atgaaaccag gaaggcggtc
     4681 tggaatatgc tactgatgta ataatacacc ttcaacagat tgatattaaa tgggattatg
     4741 cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg gagcaatatt
     4801 ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct ctctggcaaa
     4861 aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg caaccattaa
     4921 ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag cgtctactgg
     4981 ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat gagcacattc
     5041 ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac tattgtggtg
     5101 cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc cgatttgctc
     5161 cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat ccgttcgcat
     5221 taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcga atgacgaaag
     5281 cctgcgctgc gttcccacac gcagcactgg cagcactggc ggaacttcta gtgcaaaaag
     5341 aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca gcactggcag
     5401 aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca tgccagcacc
     5461 aactgacaca gccctcaaac cataccagcg ccgatctact gccagccata gtagtctccc
     5521 caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta gcgccattag
     5581 gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg gcgaaatata
     5641 tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac ctgttggcaa
     5701 ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa gcggtagttg
     5761 aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg tttcattcac
     5821 ctaccgggca ttattgacag ttggggattt tgacaacatt gccgctggag gctggagaaa
     5881 gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga atacgccatg
     5941 ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg ctatccgcag
     6001 gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc cgtcgcccgt
     6061 gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt gaaatacccg
     6121 gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga agcacaggat
     6181 aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt actgcaagaa
     6241 atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct gcttgcgctc
     6301 gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta tcagccatcg
     6361 atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa cgccctcctc
     6421 cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg attattgcag
     6481 gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctaggatct gtttaccgcc
     6541 tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt aggcgttctc
     6601 ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc tggtgaatcc
     6661 cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag tgatatcgcc
     6721 ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca ggagcgggca
     6781 aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga gcttgaagat
     6841 cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga ttttggccca
     6901 cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga tgtttccggc
     6961 agccgcctga aagacctgcc aaaaccaaac aaaagcgatg atgagtcaca ggcgaacgat
     7021 gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgccgc ccagcaggta
     7081 gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa cttccaactc
     7141 ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg gggcgtttat
     7201 agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag ctacagcacc
     7261 gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat tcctcacgtt
     7321 ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga ctacgaactg
     7381 ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc cgagcgcaac
     7441 gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt c
//
LOCUS       DADLSE010000172         7472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLSE010000172 DADLSE010000000
VERSION     DADLSE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.173"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JN692_003591"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JN692_003591"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7265791.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN692_003592"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN692_003592"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7265792.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLRMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN692_003593"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN692_003593"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7265793.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILLSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JN692_003594"
     CDS             complement(3025..3480)
                     /locus_tag="JN692_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7265794.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN692_003595"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN692_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7265795.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN692_003596"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN692_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7265796.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JN692_003597"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JN692_003597"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7265797.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQSLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAGILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1708 a         1920 c         2098 g         1746 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacgcaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gacagaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggaactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgct cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactctgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaagtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cactgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 ggcattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccgg acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tc
//
LOCUS       DADLSE010000173         7405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLSE010000173 DADLSE010000000
VERSION     DADLSE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.174"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..267
                     /locus_tag="JN692_003598"
     CDS             <1..267
                     /locus_tag="JN692_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7265798.1"
                     /translation="GDVLYPVKITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRL
                     VIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(343..807)
                     /locus_tag="JN692_003599"
     CDS             complement(343..807)
                     /locus_tag="JN692_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7265799.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAQGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEADYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(804..1679)
                     /locus_tag="JN692_003600"
     CDS             complement(804..1679)
                     /locus_tag="JN692_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7265800.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1676..2365)
                     /locus_tag="JN692_003601"
     CDS             complement(1676..2365)
                     /locus_tag="JN692_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7265801.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN692_003602"
     CDS             complement(2413..3903)
                     /gene="nanT"
                     /locus_tag="JN692_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7265802.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKDKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN692_003603"
     CDS             complement(4013..4906)
                     /gene="nanA"
                     /locus_tag="JN692_003603"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7265803.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN692_003604"
     CDS             complement(5028..5819)
                     /gene="nanR"
                     /locus_tag="JN692_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7265804.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN692_003605"
     CDS             6199..>7405
                     /gene="dcuC"
                     /locus_tag="JN692_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7265805.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHF"
BASE COUNT         1819 a         1966 c         1870 g         1750 t
ORIGIN      
        1 ggtgatgtgt tgtatccagt aaaaataacc agaaaatcat caggaaaaac tgccttccac
       61 cttgttccat tcggcttaaa taagactcac gaccttctag aggtggaaga tgcttcagaa
      121 gcaattcggc tggtgattga tgaacgtcat tccattcgct gcagtacact tacagcaact
      181 attacgaata aaaagggcaa gcgaattaag cggacaggta tatacagtat caagggtgtt
      241 aacataaaag aatacaacgt caggtaatgg cacagagcgg tccgtcagtt caggtttggc
      301 tctgtgccaa agatgggtca ggtcacacct gatctaataa atttaagcca ttaaatcagc
      361 cttaaccttc accaccacct ttttaatctc acccggctcg ccaacaacgc atcccggctt
      421 atgcggctct cctggcataa acacggcgaa cattcccggt ttcaggatga tggtttgctc
      481 gttttcaatg gcgctacaaa gctggtaatc agcttcatgg tggaactctt cacattggcg
      541 cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca gctggatatc
      601 aatgtattgc tcgtgcaatt ccgctttttt ctcgacgggc gattgagtgt taaacgtcat
      661 gacattcata aaaatattat cgccctgtaa ttcgtaacga ccctgcgctt tttcttgcgg
      721 tctggcagct aaggccagcg ttaacgcgtc ctgtaacgca ggatgtaacc cagcagacgg
      781 taatgactgt acttcaccca tcatcataat ttttctccct gggccaacag cgcagcccca
      841 agtaaacctg catcatggcg gtaatgcgcc gccagtaaat caacatgaaa tgccgctggc
      901 tcctgcgcca gatacatttc caccaacgcc agataccctt ctgccagacc aacgctgcca
      961 ccaaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag ccttgcaagc
     1021 gtacgtgcgg agcgatgaat cagttgctgc gcctgctcat cgccctgccc ggcgcgtgtg
     1081 aaaatagttc tcgcatccgc gccagccagc tcgccctgcg ctgccgccgc aatgccgcga
     1141 ccagaagcaa ttgcttccac gcaacctgtg cgtccacagc cgcagactgg gccgtgtgga
     1201 tcggcaagcg tatgcccgat gtgccccgcc agaccgccag ggccggtaag cagtttgccg
     1261 ccgctcacta caccgccgcc gacgccagtg gaaacggtga taaagaccat atcggttata
     1321 tcgccttcca gcgcctgata ctctgcccat gctgcggcct gcgcgtcgtt aatggcaatg
     1381 gtcggcaaat tggtaagttg ttccagcgtt ttgactaacg gaaagtgtag caatccaccg
     1441 agattatgcg gattcagcgc cagcaaactg ccgtcgcgaa taattccggt tgaagcgatg
     1501 gcaacccgct gcgcatgagc ttgcaacgga gagactaatg cggataaggc atcacgcaag
     1561 gcttctggtg tctggctggc tggcgtagga agttcacgac gatcgcggat ctgcccgtca
     1621 gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccag tatggtcata
     1681 gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga cgcgtaatgg
     1741 cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atctgccgcc tgagcaggcg
     1801 tgttgtaacg cccttcggca atcacccgac atccggcttc gctcaacgtt ttcaccagcg
     1861 ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta gtgccaataa
     1921 tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag tcggtcatcg
     1981 ccagtaaacc gtgatgatgg atacgtgcca gcagcgtttc aacaggcacc ggacgcgggc
     2041 ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca tcgacatctt
     2101 caatataggc cgtgatgcgt accggagaat cctccagatc gcgtttcaca attccaataa
     2161 tcggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttcg atgcgaatgg
     2221 caaccgcgcc cgcctgctct gccgctaatg ccatggctgc gacgatttcg ggtttatcga
     2281 gcgggctgtc aggaaccggc tggcaggaga caatcaggcc accgttagca gcgatttttt
     2341 gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg gtacatagac
     2401 cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc caaatggcac
     2461 ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt ccgggcgcag
     2521 ccaacgctga acgcgggaag gcatatccag accaatcagc aggatcacca caaatgtcag
     2581 gctgaacgag agcgatgcca gcgcagtacc cagatccaga cgttgagcga tcaacgcgcc
     2641 gatgattggg gccagtgcgc cacccaatgc gccaacgttg taggtaaagc ccaggcccgc
     2701 tgcacgctgg tcggtatcga aataaccgcc aatcagtttc ggtaagatcc cggcgatccc
     2761 ttgtccaagc atttgctgga agaacagtaa cagaccgagc acccagacgt ttgcgccgcc
     2821 aatcgcaaat accggaataa tcagcagctg cgaggccagc aggctacaaa cgtacgcttt
     2881 gcgggttccc agccagtcac cgaggaagcc acctacgcag catcccacgg ccgcgccaaa
     2941 gccactaaag aacagcacat tggcgacagt atgcgggtta taagccagat cggttttcag
     3001 atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcaaaca acacgaccac
     3061 catcagcatt acgcccgttg gccagcgttt acccgtactt tgcaccataa agctgataaa
     3121 gattgcggcg cataacagcc cgagaacagc gacgattgca gcattttgca ggttaccggc
     3181 gaagcagaac cacagcgcag tagccgccgc cagtgtcatt acgatattgg caatgcgatg
     3241 ttcaccacgg tagagaatat ccaccattgt gcgtactggt gctttacctg cgtgtttatc
     3301 tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga tgattggcaa
     3361 aatgccgata aagaacagcg cacgccaacc ccagaccgga accaccaggc tatagacctg
     3421 agcggcaacg acggccccca cagagaagcc tgaaatcaaa aaaccactgg ctttgttacg
     3481 cagatgtttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt cacccgccat
     3541 ccccatgccg atgaccagac gagcgataaa catggtgatg tagcctggcg caaagccgca
     3601 ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac gacgcccgta
     3661 gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa aggctgcgga
     3721 gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt cggtgagtac
     3781 cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg cagcggaaaa
     3841 tgcgcgccat tgtgcacggt tgagatggcg ataccacggg atattctggg ttgtagtact
     3901 cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt ggtatgaaaa ttgtagggta
     3961 cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaaaca actcacccgc
     4021 gctcttgcat caactgctgg gccagcgcct tcagttctgg cagatatttt tcatctaccg
     4081 gtccaaacgg tttgcggcac agcggcacag aaacgacatc catataatgc aggacagttt
     4141 tcaggccgcg gaatacgccc gttttgatca gtaaatcaat gactttgttg cactcagttt
     4201 gcagtttctg cgcggtctgg atatcgcctt ctttcagcgc cttcacgatc ccctgatatc
     4261 gccagcccat gatgttgtaa gtactgccga taccaccatc agcgcctgcc agcaagccgg
     4321 aagcgaagat ttcgtcgtaa ccgttataga gcaccagatc aggatgttca cgacggatct
     4381 gttccatctg atagagatcg ccagaagtct gtttcagcgc accgacgcca ggcaacgtaa
     4441 ccagggtgtt gatctgatcc agggtcagtt ttaccccact cagggctgga atgttgtaca
     4501 ccaccatcgg caaaccatcc gctgaatcaa taattgcccg atagtgatcg cagtgttctt
     4561 caaagctgaa aggatagtag aacggcgtga cggcagagac ggcatcgaag ccataacgtt
     4621 tagccgatgc cgcaagttgt tggctttcgg cggtgctgac gcaaccgacg tgggcgatga
     4681 gtttaatctt acctttcgcc tcttcggcga cgatttccag tacctgttca cgctcggaaa
     4741 ggctttgtac aaaggcttcg ccggtcgaac cacccacgta taaaccgtcg atgccctgct
     4801 gaatattgaa ctgaaccagg cggcgcagac tcgctttatc cagtgcttgt tgttgatcaa
     4861 aaggagtcag gagtgcagcc attacgccac gtaaattcgt tgccataaat acctctgaag
     4921 tgatgcttgt ctgataaacg atataccttt atacctgtta taccagatca attaagcaac
     4981 accccataca gaaagcttat aatgcgatct gcttcactaa agtggcatta tttctttttg
     5041 ttggtggtct gaccgaaagc gtgccaggta gcagagacgc tgttgagatg cgattgcaac
     5101 gcacgatcgg cttcgtcagg atcatgacgg cggatcgcat caacgatcgc aatatgctgt
     5161 tgataactaa cgttgttatg ttcgtgcagt gcctgatcgg taaccgttgg gcgtgcggca
     5221 ataagccagt cgagcagggc aacgtggatc gccatgaaga ttgggttacc ggggatctcc
     5281 gccagcacgc ggtggaaatc aacgtctgaa cgaatgaatg ccgcgttgtt atccagcgac
     5341 tgactgttga tttccagtgc ttttgccagc aaatcgattt gctcatcggt ggcgtgttca
     5401 gccgcatagc gcaccaggct ggattcaaag aacagacgta attgttcgaa atgggcaatc
     5461 ccaccaggat gagaaaggaa atctttcgcc atgccggaaa gctcgccgat gatggtgtcc
     5521 gcagaaggtc gagagacacg agcgcgttcg ccgttgttta tttgcaccag acctttgcgt
     5581 tttaacgctg ccagcgcttc acgcacagaa ggacgcccga cgttaaagaa cgccatcagt
     5641 tcgcgttcag acggtaattg ttcaccttcg ccaaattcac gacggcggat catctgttcc
     5701 agctcttctt ccaccatttc ggagagtttt ttacgcgcca gcgggcggct acgcaagttg
     5761 cgaccaattg caggtgaaga atcttcggtt tgcgaatcaa atgcgttcat aaggcccatt
     5821 ctgtaaggtc agtgtgatta acatcatcag tgacatccta tcacaggatt gaaagtaggg
     5881 gaaaatggca ggtttttctc tttgtgcctc atcattacca taattaacgg aataattaac
     5941 tattgcgaaa aattaatgta acgcagataa acacatcccg attgaattat tcataagact
     6001 attcacaagc attatgaata ttatgaatgt gttcttacaa aataatcata atcgcatatt
     6061 ttttaatgaa aaatcacctc acctacaatt aaaaacacga tatccgcacc ataaatagac
     6121 ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt
     6181 ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg
     6241 ggctatttga tcctgaaaaa ctacaaacct caggtagtgc tggctgccgc aggtgtcttc
     6301 ctgatgatgt gcggagtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc
     6361 ggctacttga tcgtcgacat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc
     6421 ggattggggc tgtcgattat ggcggtgggc ggttatgccc gctacatgga gcgcatagga
     6481 gccagtcgcg cgatggtgag cttgctaagc cgcccgttaa aactcattcg ctcgccgtat
     6541 attattctgt cggcaactta cgtcatcggc caaatcatgg cgcagtttat taccagcgcc
     6601 tccggtctgg gtatgttgct gatggtcacc ttatttccga cgctggtgag tctgggagta
     6661 agtcgcctct ctgcggtggc ggttatcgca accacgatgt ccattgagtg ggggattctg
     6721 gaaacgaact ccatttttgc agcccaggtc gcgggaatga aaattgccac atacttcttc
     6781 cactaccagc ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc
     6841 gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc acgaacaggc agagcaaaaa
     6901 actctcgata atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcctg
     6961 atgctcggct cgctgttcct cgcccacgtc gggctgatga agtcagaact gcatctggtg
     7021 gtggtgatgt tactaagttt gacggtgacg atgtttgttg agttcttccg caagcataac
     7081 ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgtc
     7141 aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc
     7201 actgttgatg cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg
     7261 attattatgg cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg
     7321 tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt
     7381 gtaatgatta tgccgatgca ttttg
//
LOCUS       DADLSE010000174         7311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLSE010000174 DADLSE010000000
VERSION     DADLSE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.175"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..457
                     /locus_tag="JN692_003606"
     CDS             <1..457
                     /locus_tag="JN692_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265806.1"
                     /translation="FLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAF
                     PFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVAS
                     VVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            559..2013
                     /gene="amn"
                     /locus_tag="JN692_003607"
     CDS             559..2013
                     /gene="amn"
                     /locus_tag="JN692_003607"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7265807.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2356..3072
                     /locus_tag="JN692_003608"
     CDS             2356..3072
                     /locus_tag="JN692_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7265808.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3528..3603)
                     /locus_tag="JN692_003609"
     tRNA            complement(3528..3603)
                     /locus_tag="JN692_003609"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3568..3570),aa:Asn,seq:gtt)
     gene            complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN692_003610"
     CDS             complement(3704..5347)
                     /gene="yeeO"
                     /locus_tag="JN692_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HAY7265809.1"
                     /translation="MLRYILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVQGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5352..5427
                     /locus_tag="JN692_003611"
     tRNA            5352..5427
                     /locus_tag="JN692_003611"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5385..5387,aa:Asn,seq:gtt)
     gene            complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN692_003612"
     CDS             complement(5465..6415)
                     /gene="cbl"
                     /locus_tag="JN692_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7265810.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFARKGLPV
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN692_003613"
     CDS             complement(6517..>7311)
                     /gene="nac"
                     /locus_tag="JN692_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7265811.1"
                     /translation="ELNQQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNV
                     GQSLSGQVSIGFAPGTAASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLD
                     MAVIYEHSPVAGVSSQALLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLR
                     VDEAFSLRRLTAKVIGEIESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITT
                     PSMSLSLSLNLPARANLSPQAQAVKELLMSVISSPVMEKRQWQLVS"
BASE COUNT         2059 a         1756 c         1588 g         1908 t
ORIGIN      
        1 tttccttaat attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc
       61 ctggcttgcc gatcgttttg gtcggcgcag ggtttatatc acaggcgcgt tgatcggaac
      121 gttgagcgca tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt
      181 cttctccata atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat
      241 gtttaccgaa atgtttggtg ccagttatcg ctacagtggt gctggagtcg gttatcaggt
      301 tgccagtgtg gttggcggtg gatttacacc ttttattgcc gctgcactca tcacttactt
      361 tgccgggaac tggcatagcg tcgccattta tttgctggct ggatgcctga tttctgcaat
      421 gaccgcttta ttgatgaaag acaatcaacg ctcgtgatag cctggcgaag atcatccgat
      481 cttcgcctta tacttttgtt tcacattcct gtgacatact atcggatgtg cggtaattgt
      541 atggaacagg agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg
      601 gataaactcg acgcgctgta tgagcaatct gtagttgctt tacgcaacgc cattggcaaa
      661 tatattacaa ttggcgaatt acctgatgaa aacgcccgta aacaaggtct ttttgtctat
      721 ccatcactga ccgtaacctg ggacggtagc acaacaaatc cccccaaaac gcgcgcattt
      781 ggtcgtttta ctcacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt
      841 tcgtatctga atgagcaact aacgttgctt tatcaggatt atggtgcgca tatctctgtg
      901 caaccctcgc aacatgaaat cccttatcct tatgtcatcg atggctctga attgacactt
      961 gatcgctcaa tgagcgctga tttaactcgc tacttcccga caacagaact ggcgcaaatc
     1021 ggcgatgaaa ctgcagacgg catttatcat ccaacggaat tctccccgct atcgcatttt
     1081 gatgcgcgcc gcgtcgattt ttccctcgca cggttgcgcc attataccgg tacgccagta
     1141 gaacattttc aaccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt
     1201 cgttggggat gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct
     1261 ggcgggaact ggatcactgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca
     1321 tggaaaaaac accagatgcc agcatggcat ttaattaccg ctgatggtca gggtattact
     1381 ctggtgaata ttggcgtggg accgtccaat gctaaaacca tctgcgatca tctggcagtg
     1441 ctacgcccgg atgtctggtt gatgattggt cactgtggcg gattacgtga aagccaggcc
     1501 attggagatt atgtccttgc acacgcttat ttacgcgatg accacgttct tgatgcggtt
     1561 ctgccgcccg atatacctat tccgagcatt gctgaagtgc aacgtgcgct ttatgacgct
     1621 accaagttag tcagcggcag gcctggtgag gaagtcaaac agcggctgcg tacaggtact
     1681 gtggtaacca cagatgacag gaactgggag ttacgttact cagcttctgc acttcgtttt
     1741 aacttaagcc gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat
     1801 cgtttccgcg tgccatacgg gacactactg tgtgtttcag ataaaccgtt acatggtgag
     1861 attaagcttc ccggccaggc taaccgtttt tatgaaggcg ctatttccga acacctgcaa
     1921 attggcattc gggcgatcga tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa
     1981 ttacgaacct ttaatgagcc gccgttccga taataagtta tgaaccttct tatcagaaat
     2041 agatgtgaag gattaagtaa gacatctggc aaatagcctg caataaacgc gggctgcttg
     2101 cttccttggg cagatgcgag ttttattatc gtcttaatga tttccacata ttaaaagcaa
     2161 gtatgctttc aaaacacaat tataaaaaat cccgccaaca acattagttt ttataaaatt
     2221 aaatataaga ttatggcttt agaatatttt tatttctaat agacgagatt tttcctgtta
     2281 tgatataata tgccgaatta acacatgtta acgatttacc agtaatgtaa ataaattttc
     2341 gaggagatca ttccagtggg acgtaaatgg gccaatattg ttgctaaaaa aacggctaaa
     2401 gacggtgcaa cgtctaaaat ttatgcaaaa ttcggtgtag aaatctatgc tgctgccaaa
     2461 caaggtgaac ccgatccaga attaaacaca tctttaaaat tcgttattga acgtgcaaag
     2521 caggcacaag ttccaaagca cgttattgat aaagcaattg ataaagccaa aggcggcgga
     2581 gatgaaacgt tcgtacaggg acgttatgaa ggctttggtc ctaatggctc aatgattatt
     2641 gctgagacat tgacttcaaa tgttaaccgt acgattgcta acgttcgcac aattttcaat
     2701 aaaaaaggcg gcaatatcgg agcggcaggt tctgtcagct atatgtttga caatacgggt
     2761 gtgattgtat ttaaaggaac agaccctgac catatttttg aaattttgct tgaagctgaa
     2821 gttgatgttc gtgatgtaac tgaagaagaa ggtaatattg ttatttatac tgaacctact
     2881 gaccttcata aaggaatcgc ggcgctaaaa gcagctggaa tcactgagtt ctcaacaaca
     2941 gaattagaaa tgattgctca atctgaagtt gagctttccc cagaagattt agaaatcttt
     3001 gaagggcttg ttgatgccct tgaagatgac gacgatgtac aaaaagttta tcataacgtc
     3061 gcaaatctct aattatattt taaagaaatc tgtctttatg gcagatttct ttaatctcat
     3121 ataattctta taaaaaatat aatgtttaac tcgtcatatt gcttataaaa aatccccgtt
     3181 cccagagaaa taatatttat taaaatggca ggtcttcttt ttctgattac cgaagacaaa
     3241 gtggcaatta cgtatagttt cccgataaag acgcgacagc tacatcccgc ataaggcatt
     3301 tttctcttta tctttgtacg gtacttcatg gaacagaatt tttgaccttg tgaatcgtga
     3361 tgtctgttgg ggaggggcaa tttgctcact gaagcgtgag actcgattaa gcgcacgaaa
     3421 cacagaaatc aaaaaacccg gccacttttt tacaaagtgg ccgggtaaaa ataattttta
     3481 ttttttaact gtcctgagtc tcatagagat gtctcaaaac taaaatttgg ctcctctgac
     3541 tggactcgaa ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga
     3601 ggaatcgtga gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt
     3661 ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg
     3721 gctttttttc acacttttgc ggctcggatc gagggtattt ccacagccaa cgtccagtaa
     3781 ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga
     3841 caccaaccac accccagcca agcatgattc cctgcacata accgactaca acccggcaac
     3901 cccacatgct caacatagaa acccacatgg cgtaacgggc atcacgagca cccttaaatc
     3961 cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca
     4021 gaatcacaac gacgtgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg
     4081 caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat
     4141 gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga
     4201 tcgtagaagc agaaccgagc gcacttccgg gtaagttgat aagagccgca attgagaacg
     4261 cgataaaatt tccggcaata acgctggtcc ccatcccggc aacgaacatt tgggttaata
     4321 accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt
     4381 cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag
     4441 gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg
     4501 ttaaacccaa cccagccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca
     4561 atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt
     4621 tcgtattccc tgcaccacga agtgccccgc taccaataag agtgatggct gcggctggat
     4681 aactgagtac ggtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat
     4741 cacccgcgac gaaatcaata atttgttcgc caaaatgatg aattagcgtt gccaacagta
     4801 cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat
     4861 cccgcttacc gagactaaat gctacaacga cagtagtacc aagatcgata gctgcaaaaa
     4921 aagccataat gaccatattg aagctgtccg ccaaccccac gccggccatc gcatcttttc
     4981 ccagccagct gaccagaaaa gtgctcagga cccccatcaa caggacacag gcgttctcca
     5041 tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc
     5101 gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag
     5161 ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga tgagggagaa tcagcaaagc
     5221 tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca
     5281 aacagttggg aaatttaaaa atcgggtttg acaaaatatt tttcgccgtt aagatgtacc
     5341 tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     5401 ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt
     5461 tttgctatat ctgataatca atttcctctt cactgttttc catcacctgg cgcttgatat
     5521 cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccaaacatag ttacgctgaa
     5581 gttgtcctcg cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca
     5641 ggcggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc
     5701 caagcccaag agcaacatag gttttaatga catcagaatc ctgtgcactt aatacaatat
     5761 ctaccggcaa acctttgcgg gcaaatgcgt catcagtgcg tgagcgcccc gtaattccct
     5821 gtcggtaagt aattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg
     5881 ttaagggatg atcgagtgga acaagcaaac tatggtgcca acaaaaccac gggaaggcga
     5941 cgagctgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt
     6001 gcaacaacgt cgcaatttcc tgtggcgaac cctggattag ctcgagccga acctccggga
     6061 aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg
     6121 ttgcaatagt gagaacacca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat
     6181 tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca
     6241 tgcccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt
     6301 cccgaatatg acggctaacg cctgactgtg aggtaaaaag catattcgca acctctgtca
     6361 ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa
     6421 actccgggca gttcagatat cccgttattg ttaaagtcta atgcccggca taacaaataa
     6481 taaaaacccg catcttattc catcccgata taacgcttag ctcaccaatt gccactgcct
     6541 tttttccatc actggagaac taatcactga cattaacaac tctttcactg cctgtgcctg
     6601 tggtgacaag tttgctctgg cgggtaaatt taaggacaaa gagagactca ttgaaggagt
     6661 ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcag ccgattcagg
     6721 taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa tagactcaat
     6781 ttcaccaatg acttttgccg tgagccgccg tagggaaaaa gcctcatcaa cacgaagtct
     6841 aacagcgctg taatcactgg ggagaaaaag gttcatttgc gcaatagcat taacatcaac
     6901 gttttgcccc gggcaatctt gagttcctac caggaaaaga tcttctttca gcaaagcctg
     6961 actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga gttggtgatt
     7021 tatcagtttt tcgttaagca ctgcaccact attttcatga agatagataa cgacctccgg
     7081 gaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag cggttcctgg
     7141 cgcaaagcca atcgagactt gccccgataa tgactgacca acgttatgca ccgccagttg
     7201 ggcctgctca cactgacgta aaatggcccg tgcatgggta tagagaattt ttccggcgtc
     7261 tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt tgatttaact c
//
LOCUS       DADLSE010000175         7252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLSE010000175 DADLSE010000000
VERSION     DADLSE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.176"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /locus_tag="JN692_003614"
     CDS             complement(<1..186)
                     /locus_tag="JN692_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265812.1"
                     /translation="MLQSSADKACNVLVKKVRYIKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAF"
     gene            264..1358
                     /locus_tag="JN692_003615"
     CDS             264..1358
                     /locus_tag="JN692_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HAY7265813.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1382..1594)
                     /locus_tag="JN692_003616"
     CDS             complement(1382..1594)
                     /locus_tag="JN692_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7265814.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1854..2162
                     /locus_tag="JN692_003617"
     CDS             1854..2162
                     /locus_tag="JN692_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7265815.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN692_003618"
     CDS             complement(2221..3315)
                     /gene="yaiW"
                     /locus_tag="JN692_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7265816.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPSLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN692_003619"
     CDS             complement(3328..4548)
                     /gene="sbmA"
                     /locus_tag="JN692_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7265817.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4900..6057
                     /locus_tag="JN692_003620"
     CDS             4900..6057
                     /locus_tag="JN692_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-12 family class D beta-lactamase AmpH"
                     /protein_id="HAY7265818.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6058..6561)
                     /locus_tag="JN692_003621"
     CDS             complement(6058..6561)
                     /locus_tag="JN692_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7265819.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(6649..7176)
                     /locus_tag="JN692_003622"
                     /pseudo
     CDS             complement(6649..7176)
                     /locus_tag="JN692_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1841 a         1849 c         1747 g         1815 t
ORIGIN      
        1 aaaagctatg aaatatattc tcttcgttaa cttataatta agagaaaaaa actcaccgca
       61 ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcgcac
      121 atctttgata taacgcactt tcttcaccag tacgttgcac gctttgtcag cagaagattg
      181 caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      241 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      301 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      361 aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      421 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      481 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcgcaaaacg
      541 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      601 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      661 tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc gatgccgggc
      721 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      781 aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag ggctcttctg
      841 ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat ctggcgttcg
      901 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc gaatgcgcag
      961 ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc accagcgatt
     1021 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1081 ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1141 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1201 tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1261 aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg gcgctggagc
     1321 gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc cgataatatt
     1381 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaaccct gccagccaga
     1441 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1501 ccagtacccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1561 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg aaaaacaaca
     1621 tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta attgttaata
     1681 aatttaactt aaggttgcca ttttgtgact cacaacgaag attcacgctt cgggaatatt
     1741 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1801 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1861 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1921 gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1981 acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc
     2041 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag
     2101 caggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat cgccgccagt
     2161 aaaatttacg ttgccagatg cggcctgaaa cgccttatct ggcctgacat tcctgtttga
     2221 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2281 caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2341 tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtatagcg ccgtctcctc
     2401 aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2461 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac tgtcgtaacg
     2521 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg cgttctgaaa
     2581 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt atatcggtgc
     2641 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg
     2701 actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt tggtatgctg
     2761 ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat taagcgaacc
     2821 aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2881 cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg gcgatttgat
     2941 ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     3001 ctcttgccag gcgatcttgc ttaagctcgg aacggccgga tctgcctgat aatttgactc
     3061 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3121 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3181 cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca acggttgcgg
     3241 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagatg
     3301 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3361 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3421 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3481 ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg gaaacagcaa
     3541 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt tgaaatacat
     3601 atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt
     3661 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcggc
     3721 ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagcaa
     3781 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3841 gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca ggaaggcgat
     3901 caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg aagcaaaacg
     3961 catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt gttgccagtt
     4021 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4081 gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4141 gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt gaatcagatc
     4201 atagaacggc gcataccagg cgttgacggc gaccccgact tccaccaaaa accaagtgac
     4261 gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctgtagat
     4321 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4381 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4441 cgccacccag tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4501 ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4561 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4621 gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4681 aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg attaacacgc
     4741 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4801 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4861 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4921 tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc gatcactgaa
     4981 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     5041 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg tagttatggc
     5101 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc
     5161 ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg gaccgtaaag
     5221 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac ctacaatggg
     5281 acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5341 ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac
     5401 ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc taaccttgcg
     5461 ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5521 gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc ctcaccggat
     5581 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5641 gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg gatgcagcag
     5701 tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca gacgttgatt
     5761 tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5821 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5881 acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa gaatattggc
     5941 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     6001 aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6061 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6121 gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg
     6181 atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt caatcattga
     6241 taataatgtt gcgcgggttt gtttggctgg tagatgatat cccgtacaat ttccttctga
     6301 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6361 agcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa ctccttctaa
     6421 aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct tttcattatt
     6481 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6541 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6601 gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt accaggtata
     6661 tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga ggtagttagc
     6721 atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac caacacgtac
     6781 cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat cgttgttaga
     6841 gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt cgctgtaggt
     6901 gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag actgaccgtc
     6961 aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac cagaaatgct
     7021 gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc
     7081 gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc
     7141 agacaggtcg ttgttgaagt gagagtagct caagctatga atcgccacgg ataatctaga
     7201 cacttccgag ccgttgataa tactggtttt catattctgt cggtgacatc tg
//
LOCUS       DADLSE010000176         7131 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLSE010000176 DADLSE010000000
VERSION     DADLSE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.177"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN692_003623"
     CDS             complement(32..1672)
                     /gene="pgm"
                     /locus_tag="JN692_003623"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7265820.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLSFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN692_003624"
     CDS             complement(1698..2243)
                     /gene="seqA"
                     /locus_tag="JN692_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7265821.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            2428..3192
                     /gene="ybfF"
                     /locus_tag="JN692_003625"
     CDS             2428..3192
                     /gene="ybfF"
                     /locus_tag="JN692_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7265822.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            3263..3625
                     /gene="ybfE"
                     /locus_tag="JN692_003626"
     CDS             3263..3625
                     /gene="ybfE"
                     /locus_tag="JN692_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7265823.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            3765..4295
                     /gene="fldA"
                     /locus_tag="JN692_003627"
     CDS             3765..4295
                     /gene="fldA"
                     /locus_tag="JN692_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7265824.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            4505..4591
                     /gene="uof"
                     /locus_tag="JN692_003628"
     CDS             4505..4591
                     /gene="uof"
                     /locus_tag="JN692_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7265825.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            4584..5030
                     /gene="fur"
                     /locus_tag="JN692_003629"
     CDS             4584..5030
                     /gene="fur"
                     /locus_tag="JN692_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7265826.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5114..5440)
                     /locus_tag="JN692_003630"
     CDS             complement(5114..5440)
                     /locus_tag="JN692_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265827.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN692_003631"
                     /pseudo
     CDS             complement(5477..6883)
                     /gene="chiP"
                     /locus_tag="JN692_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         1732 a         1822 c         1774 g         1803 t
ORIGIN      
        1 cttgcgaccg cccttttttt attaaatgtg tttacgcgtt tttcagaact tcgctaacaa
       61 tctcaaccgc ttctttctca atctgcttgc gatgttcttc accgaggaag ctttcgcagt
      121 agatcttata tgcgtcttcc gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca
      181 tcactttcag accgccaata gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga
      241 tcgggtcacc tgccagggtg ctggcgctca ccatttccgg agacagctta gacagcgccg
      301 ctttttgtgc ggaagtcgca gctgcctgca aacggttgta gctcggcgca ccaaagcgtt
      361 ttgccagttc gttgtagtgt tcctgcgggt tcttaccggt gacagcggtg atttccgccg
      421 ccagcagaca cataatgatg ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca
      481 ggaaggaagc ccctgcgctc tcttcgccgc caaagccgaa gctgccgtcg aacagaccat
      541 caacaaacca tttgaaacct accggtactt ctaccagctt acggcccaag tcattgacca
      601 cacggtcgat catcgcagaa gaaaccagcg ttttaccgac agcaacatct ttgccccact
      661 gcggacgatg ctggaacagg tagttgatcg ccaccgccag gtagtggttc ggattcatca
      721 aacctgccgg agtgacaata ccgtgacggt cataatccgg gtcgttagca aacgacagat
      781 cgaacttatc acgcagtgcc agcagacccg ccatcgcaca ctcggaggag cagtccatac
      841 ggatcgcacc gtctttatcg aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa
      901 cgatggtcag gttgaggttg taatactcgc caatgcgctt ccagtattcg ataccggaac
      961 cgcccaacgg atcaacgccc agcgtcaggc ccgctttctg aatcgccgcc atatcaacga
     1021 tatcggccag cccttccacg aacggctgca ccagatcctg ctctttcaca tggccggatg
     1081 ccatcgcttc gtcgagggag atacgcttca cgcctttcag gccatcggcc atcagtgcgt
     1141 tggccctgtc ttccaccact ttggtgacgt tagtatcagc cgggccacca tttggcggat
     1201 tgtacttgat accaccatct tccggcgggt tatgggacgg tgtaatcacg ataccgtctg
     1261 ccagcgggcc acctttttta ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag
     1321 tgaagccatt gttttcctgc acaatgacat caacgccgtt cgctgccagc acttccagta
     1381 cggaaatgaa cgcaggctcg gacagggcgt gagtatcttt acccacatag caagggccag
     1441 tgatgccgtt tttcgcacgt tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct
     1501 cgttaaagct gtggcgcgct gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt
     1561 gctccgcatt ccctgcttct ggtttcagta cataatattg cgccgtcagt tgggcgacgt
     1621 taatcaaatc actctgttgt gcaggttggc ctgcacgatt gtggattgcc attgctttgt
     1681 cctttgtctg caacgtttta gatagttccg caaaccttct caatcaattc cgccgggaat
     1741 tgcatcgact gcatgatgtg ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg
     1801 atcacccaat acggcgtgcc tggcacatgt ttcggcttgg tctgattacc atttttcagc
     1861 agcgtttgtt catctgccgc aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct
     1921 tcggcaaacg cctgggcgtc aagagaatat agtgtagaca acagcagcat aaagcgattg
     1981 accgctcgct tttgctctgc gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc
     2041 ttgtctttaa tcgttttgac cggcttcgct tcgacgatag caggtgacgc aacgcgaacc
     2101 tctttcgtca ccggagcagc aggctgtgat gcggcggaaa atttcaacat acgccgtaaa
     2161 atgtcggatg cgctctcgcc gatatgctta gtgtggctgg caatatagct gtagagttca
     2221 tcatcaactt caatcgtttt catcttaatc cagtgcagtg tcttagctga atacaagtcg
     2281 ccaagagtat atgaataaat cccggcagcg gatagcgtca agcctggctt gcagaaaaac
     2341 tcggaataaa ccttatttgt tgcagccctg tggcacaatg gtcaacataa taccctaacc
     2401 cgacaacgcg aaaaaaagaa ctttgccatg aaattaaata tccgcgcgca aactgcacaa
     2461 aaccagcaca ataattctcc catcgttctt gtccatggtc tgtttggtag cctcgacaac
     2521 cttggcgtac tggctcgcga tctggtaaac gatcacaata tcatccaggt tgatatgcgt
     2581 aaccacggtc tttcaccgag agagccggta atgaattacc cggcgatggc gcaggatctt
     2641 gttgataccc tggatgcact gcagatcgac aaagcaacat ttatcggtca ctccatgggc
     2701 ggtaaagcgg taatggcact tactgcactg gcccccgatc gcatcgataa actggtggcg
     2761 atcgatatcg caccggtcga ctatcacgta cgccgtcatg atgagatttt cgcagctatc
     2821 aacgcggtca gtgaatcgga cgcacaaact cgccagcaag cagcagcaat aatgcgccag
     2881 catcttaatg aagaaggggt gattcagttt ctgttgaaat cttttgttga cggggagtgg
     2941 cgctttaacg tgccggtatt gtgggatcag tatccgcata ttgtgggttg ggagaaaatc
     3001 ccggcatggg atcaccctgc cctgtttatc cctggcggca attctccgta tgttagcgag
     3061 cagtaccgtg atgatttact ggcacaattt ccacaggcac gggcgcatgt gattgcaggc
     3121 gcgggtcact gggtccatgc tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat
     3181 ctcaatgatt aacgcaactg attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc
     3241 gttggcgtga ctcatcgcgc tgatgtatta tggcgcgcta tccatccggg cagaggcctg
     3301 gctgattgtt tcccccgaag tcaccaagat catggccaaa gaacaaacgg accgtacgac
     3361 attagatctg ttcgcgcacg agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg
     3421 cgatgaacag ctgcgtatta ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct
     3481 taagcgtgtc gaactgaagc tgaacgcgga agctgtcgag gcgctgaacg agctggcgga
     3541 atcgcgtaat atgagccgta gcgaactgat cgaagagatg ttaatgcagc aactggcggc
     3601 gctgcgtagt cagggcatcg tttaaatttc cactttcatg tagcacagtg tgcagtcctg
     3661 ctcgtttgct gattccgcaa gactgcctgt tctgctatga ttgcctttat ccgtgggcaa
     3721 ttttccaccc ccatttcaat aagtttcaag aggttatttc actcatggct atcactggca
     3781 tctttttcgg cagcgacacc ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc
     3841 ttggtaaaga cgttgccgat gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag
     3901 cttacgacat tctgctgctg ggcatcccaa cctggtatta cggcgaagcg cagtgtgact
     3961 gggatgactt cttcccgact ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt
     4021 ttggttgtgg tgaccaggaa gattacgccg aatatttctg cgacgcattg ggcaccatcc
     4081 gcgacatcat tgaaccgcgc ggtgcaacca tcgttggtca ctggccaact gcgggctatc
     4141 atttcgaagc atcaaaaggt ctggcagatg acgaccactt tgtcggtctg gctatcgacg
     4201 aagaccgtca gccggaactg accgctgaac gtgtagaaaa atgggttaaa cagatttctg
     4261 aagagttgca tctcgacgaa attcttaatg cctgatgtga tgcggcgtag actcatgtct
     4321 acgccgtatt aatagataat gccaatcaaa ataattgcta caaatttgta acttttgctg
     4381 ttgtacctgt acaatgtccc agtgttcaag tggccttgcc gttgtaaatg taagctgtgc
     4441 cacgttttta ttaacaatat ttgccaggga cttgtggttt tcatttaggc gtggcaattc
     4501 tataatgata cgcattatct caagagcaaa ttctgtcact tcttctaatg aagtgaaccg
     4561 cttagtaaca ggacagattc cgcatgactg ataacaatac cgccctaaag aaagctggcc
     4621 tgaaagtaac gcttcctcgt ttaaaaatcc tggaagttct tcaggagccg gacaaccatc
     4681 acgtcagtgc ggaagattta tacaaacgtc tgatcgatat gggtgaagaa attggtctgg
     4741 ctacggtata tcgcgtactg aaccagtttg acgacgctgg tatcgtcacc cgccacaatt
     4801 ttgaaggcgg taaatccgta tttgaactga cacagcaaca tcaccacgat cacctgattt
     4861 gcctcgactg cggcaaggtt atcgaattta gtgatgattc catcgaagcg cgtcagcgtg
     4921 aaattgccgc aaaacatggc attcgcctga ctaaccacag tctctatctt tacggtcact
     4981 gtgccgaagg cgattgccgc gaagatgagc acgcgcacga aggcaaataa gccagcctga
     5041 acgagaaaag ccaacctgcg ggttggcttt tttatacaag ggaaataaga aaagcgagag
     5101 ttacagctct cacttatttg ttattactgc gtatttcctg ccagacctta tcacaatcgg
     5161 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag acactgctga taatccagta
     5221 cgcgtacact ttcctggtcg gcaaactgct gatgttgttt ctctttcttc agcacgttta
     5281 acacgctctg gcaggcttca attttttctg gcgaaccttg cgcagtgttg atacaggcgc
     5341 tgtatgcctc tttcagacgg ctgtcttcct gtggtgcggt tgattgcgca caagctacca
     5401 gccccgatgc catcatggcg atgagaatca gttttttcat tgaatgcggc aggcacaaaa
     5461 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
     5521 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
     5581 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
     5641 agaccgcatc caggctgtag gcagactctt caatagtccg ttttttgtcg tagtacgcat
     5701 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
     5761 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
     5821 tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
     5881 aggaagcgta ggttggggtc atacgttgca ggaagtatcc ctgctgactg tcagccttaa
     5941 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
     6001 cttgcagcta ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
     6061 gggtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
     6121 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
     6181 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtgg
     6241 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
     6301 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
     6361 ccgccccctg ataagtaccc ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg
     6421 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
     6481 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
     6541 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
     6601 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatagaggt
     6661 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
     6721 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
     6781 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcaa
     6841 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
     6901 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg
     6961 ttgagaaaag tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc
     7021 tctttctgta accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt
     7081 acaaggctta agtttgcggt tgaaaggctt gcagaagaat aaaaacgtga a
//
LOCUS       DADLSE010000177         7125 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLSE010000177 DADLSE010000000
VERSION     DADLSE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.178"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            154..1197
                     /locus_tag="JN692_003632"
     CDS             154..1197
                     /locus_tag="JN692_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7265828.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            1712..2173
                     /locus_tag="JN692_003633"
     CDS             1712..2173
                     /locus_tag="JN692_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265829.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(2170..3025)
                     /locus_tag="JN692_003634"
                     /pseudo
     CDS             complement(2170..3025)
                     /locus_tag="JN692_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3022..3321)
                     /locus_tag="JN692_003635"
     CDS             complement(3022..3321)
                     /locus_tag="JN692_003635"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7265830.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
     gene            3783..4040
                     /locus_tag="JN692_003636"
     CDS             3783..4040
                     /locus_tag="JN692_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265831.1"
                     /translation="MKNYPTYGFYYPIANFIALQINGSKTGFAASITGFTQTISAGTI
                     SFLSVTVSDNGVGPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(4681..4953)
                     /locus_tag="JN692_003637"
     CDS             complement(4681..4953)
                     /locus_tag="JN692_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265832.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            5042..5221
                     /locus_tag="JN692_003638"
     CDS             5042..5221
                     /locus_tag="JN692_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265833.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            5273..5467
                     /locus_tag="JN692_003639"
     CDS             5273..5467
                     /locus_tag="JN692_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265834.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(6266..6602)
                     /locus_tag="JN692_003640"
                     /pseudo
     CDS             complement(6266..6602)
                     /locus_tag="JN692_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2069 a         1601 c         1508 g         1947 t
ORIGIN      
        1 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
       61 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
      121 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
      181 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
      241 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
      301 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
      361 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
      421 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
      481 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
      541 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
      601 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
      661 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
      721 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
      781 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
      841 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
      901 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
      961 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
     1021 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
     1081 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
     1141 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
     1201 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
     1261 cttccccaag tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
     1321 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
     1381 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
     1441 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
     1501 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
     1561 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
     1621 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
     1681 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
     1741 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
     1801 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
     1861 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
     1921 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
     1981 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
     2041 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
     2101 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
     2161 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
     2221 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
     2281 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
     2341 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
     2401 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
     2461 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
     2521 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
     2581 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
     2641 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
     2701 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
     2761 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
     2821 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
     2881 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
     2941 agccgctgcg ggcaacccga agtacacgac acatcgcttt aatgctgaac tctgcccgat
     3001 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
     3061 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
     3121 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
     3181 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
     3241 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
     3301 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
     3361 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
     3421 atcatctgta agcgcagtaa taccattagt agcacctctc ggcggcgcat tgattattga
     3481 atattataac tggaatgtta tatttcttgt tatgatgctg ttcagtatta taataatgtt
     3541 attatcacca aagataactc atgcaaatga gaaagtaata tgtcaaaaca gtgcatcctt
     3601 tgtgaccaaa gagtttatct gcggcactat acttgtctca ttaatactca gttctctatt
     3661 ttgctacata tcaatatcac catcaatttt catgcatact tatggcctga gtacttttag
     3721 ttactcaatt attttctcct tctcagtttt atttttcata atcggaaatc aattatcaaa
     3781 gattgaaaaa ttatccaacc tatggttttt attacccaat agccaatttt atcgcattac
     3841 aaataaatgg ttcaaaaaca ggatttgccg catctattac aggttttact caaacaataa
     3901 gcgctggtac aatcagtttc ctgtcagtaa ccgtaagcga caacggtgtt ggtcccggga
     3961 ctactttagg tcttggttca ctaataactt cattactgtc aatagccgta atcgccattg
     4021 caggaaataa taaaaagtaa gtgtactctg actgcgtatg tatctacttt gtgtaatgca
     4081 ggtgtctatt gaaaataatt cttttcgtgg gcatctgttg tataaaataa aaaattatcg
     4141 taagtactcc atctgcgatg tcttgtgaaa attatcctgt ctggcaacaa tcgcgtccat
     4201 ctgtattaat ggactcgaac gatgaactct caaacaacac tagatatcac cggctttcgc
     4261 acttatttgc cagaatcatt acgtctcaga ttcgacgagc aattatctgt ccgggctatc
     4321 gcacagcagt taagactcag ttattccccc attcacaatc tctttcaacg aattaacgcc
     4381 tcaggtattg tctgaccatg acccgagtcc ctttcattcg cccaaccgat accatacttt
     4441 atgccaacag aaagaatgaa tgaaccgcgc ctaaaatcag cgaggacaca tggcacaaag
     4501 tgcgacatgt ctcacattta accccagtgg gggcaacaag tataaaaccg ttttttgatt
     4561 aagtggtcgg atatgaaaag gggaaggttt atgtgaaaaa tacctgtgtt ttctttgaaa
     4621 acacacaaca gtatcaggtt atttatgttt atacagtgcg ttccaaactg acctgaataa
     4681 ttacgcaagt tgcaacaata aaacattttt gccgtagtgt gaggtactcc ggtaaattgc
     4741 tttccaggtg cagacaatga ctaacgaaga actacaggca atcgcattat ccatgcttga
     4801 gcgccagcgc gaaaaagtac accagcaacg agagtctttg cttaacccat ctccattcac
     4861 caggccaaaa cgattggagc ttgaagcatt cttggacaaa tacccacggc ggcttaaatc
     4921 aggcaaaacc agaccaccag gctgatagct cacagtttgt tctccgattc aataaaacgg
     4981 ataagaacca tcagcgcggt agcgtaaggc actacagcgc attgctttac gagtaataac
     5041 aatggattta tcaccagaag acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag
     5101 agaagccatt atcgaacagc aaacgggcat gagcgccata gacccattgc gatacaaacg
     5161 ggcctcatct cctttagttg acgacaattc caatgcggat aaaacacgtc caccacactg
     5221 attacatttt tcattagcac agtggtgtga tgcagcatac gaggaaaacc ggatgaatga
     5281 gaacaaaata aaaaggctgg agcaactgtt acaggcacgc caacgggagt ttgccactaa
     5341 atggggacgt gccgcgctgc cacatgaacg cctaacctat tttccacttg aagacctcat
     5401 taaaaataat ggcaaaaaaa tcaggtctga taagaaacac ttcttagaat gcaatgaaga
     5461 caaataacaa caatctgcat attgccacca accacagatg tggcatcgtt atcagcgcac
     5521 ttgattttct ttatatccac tgacgagctt gccggggcac ctttgtttgc cggtcattct
     5581 ggtatcatgc agatatttac ggcgtacaag cccactagca tgacattgta ataaggcctg
     5641 agcctaacgc tttaatgtgt gtcatacact cagtggcgta atcgttgaca atatggtgca
     5701 tgtaatcgta agcgtccagc gaaatccatc tggtcataat ctgaagcatc cgacaaagtg
     5761 gtgtccacca aataagtgat ctgcgcccaa tatcctgagc cggaactttg acaaaggcca
     5821 acgaaaaatg ggagcggatg ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg
     5881 ccagttctcc gatctgttta accgggaaat tatctcctac agcttgtcag aaaggtcggt
     5941 gatggagcat cgttaatacg atgctatacg atgcattcac agtgcccggc cagaggatgc
     6001 cccgtcgctg catatggatc agggttggca atatcgaatc gcaggctata ggcaaagtta
     6061 aagccccatg gagtagcaca aaatatgccg cacaaaggaa acggtctgaa taacgcagtg
     6121 atgaagaact tcttcagcac actgctaaaa cgcatagtga tcgagcgctg attctggcga
     6181 acaactgaac taatacatca gaatctgcat tatgttaaat aaatatgaaa agatggttta
     6241 aataccccgt tacttttgac ttacactata cggtatcgca tcgtttaata ttcgcaccgg
     6301 ccagattttt atttctatta gttgtcacaa tactgaatgc gtacgaccac agtattctgg
     6361 ctcctgtgtg gttatgcttt aattctgcgt tccgggcaga taagcagttg cttgcaggaa
     6421 tccttcttgt gttaatgtca gttccccttt ttaccagtgc tgatttccac attccgtcca
     6481 acagagctta tagcctttcc ctggattata gcattgtccg gctgaagttc tttttgaata
     6541 ataatagaag cactgctggc agatccagtc cgtttttcat aacccactgt actgataacc
     6601 ataatctaat cagtagaaat tgagtcaaaa ataagcacta ctccatacag gataattaga
     6661 gatcagtttg attattcaca attcatcatc agcattttct atttctgacg aaatcaacat
     6721 gaaaataacc atatatgata attattataa taacggtttt aattggaata catatattac
     6781 aacgtattat atataattgg tattctggga actatattct caaaatacag tagaaacgag
     6841 gtatgtttct ggtggaaagg acagttggat taaaaagtaa gaattgataa aaaaaaacgc
     6901 cagcaacaca tagtgccggc ggagggaata ccccatggag aaaatgtgat gcctagagca
     6961 tcatgaatat ataattaaaa atagttagcg ttgtcaatac tttcacaaaa ataattttcg
     7021 tagtatagaa agatattttt atgcatgacc tacctgaatt tgctccgggt agaggttata
     7081 aataaaaatt gaatcacgac aaacacaata ttcatagtat ggcga
//
LOCUS       DADLSE010000178         7124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLSE010000178 DADLSE010000000
VERSION     DADLSE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.179"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..273
                     /locus_tag="JN692_003641"
     CDS             <1..273
                     /locus_tag="JN692_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="allows for ions and hydrophilic solutes to cross
                     the outer membrane; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7265835.1"
                     /translation="QFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVG
                     ATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            414..848
                     /gene="uspF"
                     /locus_tag="JN692_003642"
     CDS             414..848
                     /gene="uspF"
                     /locus_tag="JN692_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7265836.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1024..1959
                     /gene="ttcA"
                     /locus_tag="JN692_003643"
     CDS             1024..1959
                     /gene="ttcA"
                     /locus_tag="JN692_003643"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7265837.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN692_003644"
     CDS             complement(2088..3461)
                     /gene="dbpA"
                     /locus_tag="JN692_003644"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7265838.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN692_003645"
     CDS             complement(3939..4922)
                     /gene="zntB"
                     /locus_tag="JN692_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7265839.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGSLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5177..6409
                     /gene="dgcM"
                     /locus_tag="JN692_003646"
     CDS             5177..6409
                     /gene="dgcM"
                     /locus_tag="JN692_003646"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HAY7265840.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLTA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHNPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARPLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN692_003647"
     CDS             complement(6430..6993)
                     /gene="smrA"
                     /locus_tag="JN692_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7265841.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKAAQAKQENWERHAKRSR"
BASE COUNT         1850 a         1825 c         1678 g         1771 t
ORIGIN      
        1 cagtttgatt ttggtctgcg tccggcagtc tctttcctga tgtctaaagg ccgtgacctt
       61 cacgctgctg gtggtgcaga caacccggca ggtgttgatg ataaagatct ggttaaatac
      121 gccgatgttg gcgcgactta ctatttcaat aaaaacatgt ccacctatgt tgactataaa
      181 atcaacctgt tggatgaaga tgacagtttc tacgctgcca acggcatctc taccgatgat
      241 attgtcgctt taggtctggt ttatcagttc taaatcctcc tgcccgctgt tatggcgggc
      301 tttttctgct tattcttcct cctttgatct aaattaaaaa tgtgaactcc gtcattacac
      361 aaaaagtgtc atctggcgtt acactttatg cggatactaa aacaggaggt tttatgaaca
      421 gaacgattct tgtccctatc gatatttcag attcagaatt aactcaacgc gtgattagcc
      481 acgttgaggc agaggcaaag attgatgatg cagaggttca tttcctgacg gtaatacctt
      541 cactgcccta ctatgcctct ctgggtttag cgtattccgc agaattaccg gcaatggatg
      601 acctgaaagc ggaagccaaa tcgcaactgg aagagatcat taaaaaattt aaactgccaa
      661 ccgacagagt gcatgtccat gttgaggaag gctcgcccaa agaccgcatt ctggaattgg
      721 cgaagaagat ccccgctcat atgatcatca ttgcttccca tcgaccggat atcaccactt
      781 atctgctcgg ttccaacgcc gcagctgtag tgcgtcacgc agagtgctcc gtgctggttg
      841 tgcgctgaca ctaacgcccg cacattgctg cgggcttttt gattcatttc gcaaatgtgc
      901 tgacattttc ccctctaatc cgtaccatac acgccacagt ttttatatca gatttcttat
      961 gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt gagtcgcttt
     1021 taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa caaattacaa
     1081 aaacgtctgc gtcgtaacgt gggcgaagcc attgctgact tcaatatgat tgaagaaggc
     1141 gatcgcatca tggtttgcct ctccgggggt aaagacagct ataccatgct ggagattctg
     1201 cgcaatttgc agcaaagcgc gccaatcaat ttttcgctgg tggctgttaa cctcgatcaa
     1261 aagcaaccgg gcttcccgga acacgttctg cccgagtatc ttgaaaagct gggcgttgag
     1321 tacaagattg ttgaagagaa cacttacggt atcgtgaaag agaagattcc agagggcaaa
     1381 accacttgct cactgtgttc tcggctacgt cgcggtatcc tttatcgcac cgcaacggaa
     1441 ctggggacga cgaagatcgc gttgggtcac catcgtgacg atatcctgca aacgttgttc
     1501 ttaaatatgt tctacggcgg caagatgaaa ggtatgcctc cgaaactgat gagcgatgat
     1561 ggcaaacata tcgttattcg tccactggcc tactgccgcg agaaagatat tcagcgattt
     1621 gccgatgcaa aagcgttccc gattattccg tgcaacctgt gcggttcaca gcctaacctg
     1681 caacgtcagg tgattgctga catgttgcgt gactgggata aacgttatcc agggcgtatc
     1741 gagacgatgt tcagcgcgat gcagaatgtg gtgccgtcgc atctgtgcga taccaacctg
     1801 ttcgatttca aaggcattac ccacggttct gaagtggtta acgggggtga tctggcgttt
     1861 gatcgcgaag agatcccact acaaccggcg ggctggcagc cagaagaaga tgaaaatcag
     1921 ttggatgaat tacggctgaa tgtggttgaa gtgaaataac caggatagcg cccgatgcgc
     1981 aagcgtatcg ggctactctt atggaggccg gataagacgc ggccagcgtc gcatccggca
     2041 atcccgaata agatgtttac tcttgcaccc ggcaattcaa catttcatta ttttaataac
     2101 cgcacccggc acgtttttcc tttaatcttc ccgccctgta actgtttcca tgctttatga
     2161 gcaacagcct gacggaccgc gacatagaca tgcgccggat gcacggcgat tttgccaata
     2221 tctgcgccat caagcccgat atctcctgtc agtgcaccta atacatcacc cgggcgcatt
     2281 ttggcttttt tcccgccatc gatacacaac gttgccattt ctgcttccag cgtcacaatg
     2341 gaactattag ctggcggcgt ttgccagtta agttttatct gcaacatgtc agaaatgata
     2401 ttggcccgct gtgcttcttc cggagcacag aaactgatcg ccagaccgct atttcctgca
     2461 cgagctgtac gaccgatgcg atgtacatga acttcagggt cccacgccag ctcaaagttc
     2521 accaccagct caagcgattt aatatccaga ccacgcgcag caacatcagt cgcaaccagt
     2581 acacgggcgc taccgttagc aaaacgtacc agggtctgat cgcgatcgcg ttgctccaga
     2641 tcgccgtgta acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg
     2701 caatcttttt tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac
     2761 cgttgcaaca gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt
     2821 ggcaaagcat ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg
     2881 attgcggcga tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc
     2941 gcaaaacgga tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc
     3001 tcatccatca ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc
     3061 agcaaacgac ccggcgttgc cacgataata tgcggcgcat gttgcaacga atcacgctgc
     3121 ataccgaacg gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc
     3181 agccgacgca attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa
     3241 gcctgggttt gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc
     3301 gttttgccgc tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga
     3361 agcgcggcgg cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt
     3421 tgggcgggag gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat
     3481 cgacgcaaag ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa
     3541 tcggttcttc atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca
     3601 ctggaacagg atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg
     3661 tcatatttcc ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg
     3721 caaaaaaaag ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat
     3781 tcaaaaaagg aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc
     3841 tgcaagggag atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg
     3901 gcgtttttgt atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc
     3961 aaccataaag caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg
     4021 aattgccacc cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag
     4081 gtactgggta aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt
     4141 tcctgcatca cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg
     4201 atttcgtcaa ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca
     4261 tcgctcatcc acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc
     4321 atatagcgac gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga
     4381 atttgctgat caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata
     4441 aattcactgg aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg
     4501 gtcggacccg tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt
     4561 tgtcgggtcg aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg
     4621 gggcgttcat cggtgctgcc gtttatacag cgcaatgtaa tcagcgagcc ttcaccgaaa
     4681 cggctgactc ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc
     4741 agcggtgttg tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag
     4801 cagggatgcg cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca
     4861 tccagcatcc aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc
     4921 acagtgcctc catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa
     4981 catctcatca ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa
     5041 aagcaaaatg cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc
     5101 agaattatct gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa
     5161 aaataacggg atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc
     5221 tgtctggatt gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact
     5281 gttgatgcaa gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca
     5341 aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct
     5401 cactgttcag cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct
     5461 gacagaaaca gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt
     5521 aaaagccagt cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt
     5581 tctgactaac tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga
     5641 tgctaacctc accgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca
     5701 tacctgggaa ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca
     5761 tttacccggc ggtcataacc ctttgaattt tgttcataaa ctggcggatg gatctactcg
     5821 tcatgtgcag acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat
     5881 tgtgcatgat attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca
     5941 tgacgcgatg accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca
     6001 aatgcagcat ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt
     6061 taaacacatt aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc
     6121 ccgccccctc gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga
     6181 gtttgtctta ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat
     6241 ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg
     6301 tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc
     6361 tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc
     6421 gtctcgagat caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc
     6481 cgctttacgt agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca
     6541 atatgcctga acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt
     6601 ggcatgtgat ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc
     6661 cgccagggct tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg
     6721 caaaagattc aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc
     6781 atgctgcaac ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa
     6841 tccggtagtg aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt
     6901 acgcgttgga tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc
     6961 gtcaagaaac agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc
     7021 gtgattcaga cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca
     7081 atactctact tcatacctgt gagtatctgc gcaggcgggg catt
//
LOCUS       DADLSE010000179         7015 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLSE010000179 DADLSE010000000
VERSION     DADLSE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.180"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            242..1219
                     /locus_tag="JN692_003648"
     CDS             242..1219
                     /locus_tag="JN692_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7265842.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1267..1530)
                     /locus_tag="JN692_003649"
                     /pseudo
     CDS             complement(1267..1530)
                     /locus_tag="JN692_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
     gene            complement(1565..3316)
                     /locus_tag="JN692_003650"
                     /pseudo
     CDS             complement(1565..3316)
                     /locus_tag="JN692_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            3320..3712
                     /locus_tag="JN692_003651"
     CDS             3320..3712
                     /locus_tag="JN692_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY7265843.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3850..4734
                     /locus_tag="JN692_003652"
     CDS             3850..4734
                     /locus_tag="JN692_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY7265844.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN692_003653"
     CDS             complement(4766..5965)
                     /gene="tet(A)"
                     /locus_tag="JN692_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7265845.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN692_003654"
     CDS             6071..6721
                     /gene="tetR(A)"
                     /locus_tag="JN692_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7265846.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6753..6995)
                     /locus_tag="JN692_003655"
     CDS             complement(6753..6995)
                     /locus_tag="JN692_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7265847.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLMRWKGGGDVVYMALL"
BASE COUNT         1305 a         2151 c         2217 g         1342 t
ORIGIN      
        1 acgccttcag tagctgagca ggagggacaa ctgatggaga tggaagccac gggagcacct
       61 caaaaccacc atcatacact aaatcagtaa gttggcagca tcaccgtaat cgtacagcta
      121 agaacaagtg cttcaagtct acttattaaa atgtaccttt gaaaaagtag actttctgcg
      181 gtacgctctg acagtaagtt tgcccccgat tttttacgca gcgcgccgga cggagcccga
      241 catgatcctg attcccgatg acgaaccgga gctgagtctg cccgcagcca gtgaggaatt
      301 cctgccggcg ctgtcaggtg agaatgcccc ggtcagcccg gcccgggcct acctgctttc
      361 actcaattcc caccgcagcc ggcagaccat ggcctcgttc ctgaacatcg tcgccgttat
      421 gctcggagct gcctccctgg agtcctgcag ctggggcagc ctgcggcgcc atcacgtgat
      481 ggccgtgacc gaactgctgc gcgacaccgg ccgggccacc gccaccgtca acacctatct
      541 ttcggcactc aagggcgtgg cgaaggaagc ctggatgctc cggctcatgg atgtggagag
      601 cttccagcat atccgggcgg tccgtaacct gcgcggcagt cggctgccca gcggtcgggc
      661 gctgccccag ggggagatcc gcgccctgtt tgccgtctgt gaagccgatc gcagctgcct
      721 cggggcgcgg gatgcagcga tgctggcggt cattctcggc tgcggcctgc gacgatccga
      781 agtagtgagc ctggatttgc gtgacgttgt cactcaggac cgtgcgctta gagtgctggg
      841 taagggaaac aaggagcgac tggcatatgt cccggccggt gcctggcagc ggctgcagat
      901 ctggatcgat gagatccggg gcgagacccc gggcccgctg ttcacacgca tccgtcgttt
      961 cggggatgtg actctaaacc ggctaaccga ccaggcggtg taccatatcc tgcaggtgcg
     1021 ccagggtcag gccggcatca cgaaatgttc tccacacgat ctgaggcgaa ccttcgccac
     1081 cgcgatgctg gataacgggg aggatttaat taccgtgaag gatgcgatgg gccacgcgag
     1141 cgtcaccacc acccagcagt atgaccgtcg cggggagcaa cgcctgcagg acgcgcggga
     1201 tagactcaac cttatttagg gctaagtaaa cacaaacaag ggggctgtac cccctctaat
     1261 tttacattaa cacgatactc gacagacttt cccagtcgta gtcagcaacc atcgcccgat
     1321 ggaggctggg ataaatgctg acagcccgac gtgaagcaaa aggctctcgc cgctggcatg
     1381 gcttaatgtc gcggtcccct caatcactaa cacaatttca gcgctggatg tcattaccgt
     1441 ttgattgttc accgcagtga aaatactgaa atggaaatcc tcaacaggaa taggatagcg
     1501 cgcttctgcg gcgtgctgtt ccggtaacat aggggagccc gcagaattcg gaaaaaatcg
     1561 tacgctaagg ttttccgggc atccgtaagg gccgaaactt cccgtcttcc agtctgcggc
     1621 tctgccgcca gacgtaatcg ccggttaggt tgatgtgctc ccagcccagc ggcgacagga
     1681 attgcagcag ctcgccgtcc accggcttgc cggcctcgac caacccctgg gtggcgcgtt
     1741 ccaggtacac cgtgttccac agcacgatag ccgccgtcac caggttgagg ccgctggccc
     1801 ggtagcgctg ctgctcgaag ctccgatccc tgatttcccc aaggcggttg aagaacaccg
     1861 ccctggccag cgagttgcgc gcctcacctt tgttcaggcc ggcatgcacg cggcggcgca
     1921 gttcaacact ttgcagccag tccaggatga acagcgtgcg ctcgatccgg cccagctcgc
     1981 gcagggccac ggccagtccg ttctggcgcg ggtagctgcc gagcttgcgc agcatcagcg
     2041 aggcggtgac ggtgccctgc ttgatcgagc tggccaggcg caggatgtcg tcccagtggg
     2101 cacgcacgtg cttgatgttc agggtgccgc cgatcagcgg gcgcaacgtc gggtaggctt
     2161 gcacgccctg cggcacgtac agcttggttt cgccgaggtc gcggatgcgc ggcgcgaagc
     2221 ggaagcctag caggtgcatc agggcaaaga cgtgatcggt gaagccggcc gtgtcggtgt
     2281 agtgctcctc gatccgcagg tcggactcgt ggtacagcag gccgtcgagc acataggtgg
     2341 aatcgcggac gccgacattc accacgcggg tgctgaacgg cgcgtactgg tcggagatat
     2401 gggtatagaa cagccgtccc ggctcgctac cgtacttcgg gttgacgtgc ccggtgctct
     2461 cgccccggcc acccgcgcgg aagcgctggc catcggagga tgaggtcgtg ccgtcgcccc
     2521 agtgggcggc aaaggcgtgg cgatactggt ggttgaccag ctcggccaag gccgccgaat
     2581 aggtttcgtc gcggatgtgc caggcttgca gccaggacag cttggcgtag gtcaggccgg
     2641 ggctcgactc ggccatcttg gtcagcccga ggttgatcgc atcaccgagg attgcggaca
     2701 gcagcaacgt cctgtctttg gcctcggccc cgtccttcaa gtgggtgaag tggcggctga
     2761 agcccgtcca gtcgtccacg tccatcagca gttcggtgat cttgatgcgc ggcagtaact
     2821 ggctggtttg gtcgatcagc gcctgcgccc gatccggcac cgccgcatcc agcggggtga
     2881 ttttcagccc tgactcggtg aggatggcat cgggcagctc gttgtccttg gccaggcggg
     2941 tgacggtggc caactgctcg tccagcagct gcaaacgctc ttccaggtac tggtcgctgt
     3001 tcgggttgat cgccaggggc agggcctgct cgcgcttgag tgcggcgaac ttctcggccg
     3061 gcagcaggta gtcgtcgaag tcgcggaact gccgcgagcc cttgacccag atgtcgccgg
     3121 agcgcagggc gttcttcagc tcggacaggg cgcagatttc gtagaatttc cggtcgaggc
     3181 cttccggggt gatcaccagc ggcttccagc gcggcttgat gaaggccgtg ggtgcatcgg
     3241 ccggcacctt gcgcaggttg tcggcgttca tctcacgcag ggtctgcacg gctgccagca
     3301 cgccttgcgc ggccggcccg tgctgttccc caacttttcg ctcggcgaag agtatgaaca
     3361 tgcgccgccg gcgacgaatc gccaaatctc accgtatctc ccgagcggac gatttcgcac
     3421 cggtctgccc gtcgaagggc ttgcgatcga acggggcgac cttttctatg catgtccgcg
     3481 agccagcgtc ttttatggca cggcgctcga cgccgacctt cggacgcgcg gcgtaagcac
     3541 gcttgtcatg gccgggataa gcaccaccgg cgttgttctt tcaagcgtcg cctgggctag
     3601 tgatgcggac tacgacgtgc gtttggtcca ggactgctgc tacgacccgg atcgggatgc
     3661 ccacgaagct ctgttgcgtt ccgggttcgg cggacgtgta caggtcgtgt aattttcggg
     3721 cctcgcatga tcgcggccat agccgcaggt ggattggtgc gaggcagggc cagtccaagt
     3781 cagggtgcgg tcatcgcggc gcctgcaact ctcaaaaacc catcgccgta gcggaggtct
     3841 tgtaatcgca tgtcattacg cacgcccgat ttattgttca cagcgatagc acctgccatt
     3901 tggggcagca cctacattgt caccacccaa tacctgccga acttctcacc gatgacggtc
     3961 gcgatgctgc gggcgttgcc ggcgggttta ttgctcgtga tgatcgtccg acagattcca
     4021 acgggaatct ggtggatgcg catcttcatc ctcggcgcac ttaatatttc gctattctgg
     4081 agcttgttgt ttatttcggt ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct
     4141 gtgcagccgc tgatggtcgt gttcatctct gccgctctgc taggtagccc gatacgattg
     4201 atggcggtcc tgggggctat ttgcggaact gcgggcgtgg cgctgttggt gttgacacca
     4261 aacgcagcgc tagatcctgt cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg
     4321 ttcggaaccg tgctgacccg caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc
     4381 tggcaactgg cggccggagg acttctgctc gttccagtag ctttagtgtt tgatccgcca
     4441 atcccgatgc ctacaggaac caatgttctc ggcctggcgt ggctcggcct gatcggagcg
     4501 ggtttaacct acttcctttg gttccggggg atctcgcgac tcgaacctac agttgtttcc
     4561 ttactgggct ttctcagccc ggggaccgcc gtgttgctag gatggttgtt cttggatcag
     4621 acgctgagtg cgcttcaaat catcggcgtc ctgctcgtga tcgggagtat ctggctgggc
     4681 caacgttcca accgcactcc tagggcgcgt atagcttgcc ggaagtcgcc ttgacccgca
     4741 tggcataggc ctatcgtttc cacgatcagc gatcggctcg ttgccctgcg ccgctccaaa
     4801 gcccgcgacg cagcgccggc aggcagagca agtagagggc agcgcctgca atccatgccc
     4861 acccgttcca cgttgttata gaagccgcat agatcgccgt gaagaggagg ggtccgacga
     4921 tcgaggtcag gctggtgagc gccgccagtg agccttgcag ctgcccctga cgttcctcat
     4981 ccacctgcct ggacaacatt gcttgcagcg ccggcattcc gatgccaccc gaagcaagca
     5041 ggaccatgat cgggaacgcc atccatcccc gtgtcgcgaa ggcaagcagg atgtagcctg
     5101 tgccgtcggc aatcattccg agcatgagtg cccgcctttc gccgagccgg gcggctacag
     5161 ggccggtgat cattgcctgg gcgagtgaat gcagaatgcc aaatgcggca agcgaaatgc
     5221 cgatcgtggt cgcgtcccag tgaaagcgat cctcgccgaa aatgacccaa agcgcggccg
     5281 gcacctgtcc gacaagttgc atgatgaaga agaccgccat cagggcggcg acgacggtca
     5341 tgccccgggc ccaccggaac gaagcgagcg ggttgagagc ctcccggcgt aacggccggc
     5401 gttcgccttt gtgcgactcc ggcaaaagga aacagcccgt caggaaattg aggccgttca
     5461 aggctgccgc ggcgaagaac ggagcgtggg gggagaaacc gcccatcagc ccaccgagca
     5521 caggtcccgc gaccatcccg aacccgaaac aggcgctcat gaagccgaag tgccgcgcgc
     5581 gctcatcgcc atcagtgata tcggcaatat aagcgccggc taccgcccca gtcgccccgg
     5641 tgatgccggc cacgatccgc ccgatataga gaacccaaag gaaaggcgcc gtcgccatga
     5701 tggcgtagtc gacagcagcg ccggccagcg agacgagcaa gaccggccgc cgcccgaaac
     5761 gatccgacag cgcgcccagc acaggtgcgc aggcaaattg catcaacgca tacagcgcca
     5821 gcagaatgcc atagtgggcg gtgacgtcgt tcgagtgaac cagatcgcgc aggaggcccg
     5881 gcagcaccgg cataatcagg ccgatgccga cagcgtcgag cgcgacagtg ctcagaatta
     5941 cgatcagggg tctgttgggt ttcacgtctg gcctccggac cagcctccgc tggtccgatt
     6001 gaacgcgcgg attctttatc actgataagt tggtggacat attatgttta tcagtgataa
     6061 agtgtcaagc atgacaaagt tgcagccgaa tacagtgatc cgtgccgccc tggacctgtt
     6121 gaacgaggtc ggcgtagacg gtctgacgac acgcaaactg gcggaacggt tgggggttca
     6181 gcagccggcg ctttactggc acttcaggaa caagcgggcg ctgctcgacg cactggccga
     6241 agccatgctg gcggagaatc atacgcattc ggtgccgaga gccgacgacg actggcgctc
     6301 atttctgatc gggaatgccc gcagcttcag gcaggcgctg ctcgcctacc gcgatggcgc
     6361 gcgcatccat gccggcacgc gaccgggcgc accgcagatg gaaacggccg acgcgcagct
     6421 tcgcttcctc tgcgaggcgg gtttttcggc cggggacgcc gtcaatgcgc tgatgacaat
     6481 cagctacttc actgttgggg ccgtgcttga ggagcaggcc ggcgacagcg atgccggcga
     6541 gcgcggcggc accgttgaac aggctccgct ctcgccgctg ttgcgggccg cgatagacgc
     6601 cttcgacgaa gccggtccgg acgcagcgtt cgagcaggga ctcgcggtga ttgtcgatgg
     6661 attggcgaaa aggaggctcg ttgtcaggaa cgttgaagga ccgagaaagg gtgacgattg
     6721 atcaggaccg ctgccggagc gcaacccact cactacagca gagccatgta gacaacatcc
     6781 cctccccctt tccaccgcat cagacgcccg tagcagcccg ctacgggctt tttcatgccc
     6841 tgccctagcg tccaagcctc acggccgcgc tcggcctctc tggcggcctt ctggcgctcc
     6901 tgctgcggcg tccgctcgtg ggccgtggcg cgggtccgcg cgccggcctc gtgcgcctgg
     6961 cgctcgcggg cgaggtccag ggcggccgtc ttcacgttct gccttgcgca gatga
//
LOCUS       DADLSE010000180         7012 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLSE010000180 DADLSE010000000
VERSION     DADLSE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.181"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..796)
                     /locus_tag="JN692_003656"
     CDS             complement(<1..796)
                     /locus_tag="JN692_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY7265848.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETAN"
     gene            complement(883..1244)
                     /locus_tag="JN692_003657"
                     /pseudo
     CDS             complement(883..1244)
                     /locus_tag="JN692_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1584..2138)
                     /locus_tag="JN692_003658"
                     /pseudo
     CDS             complement(1584..2138)
                     /locus_tag="JN692_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2281..2403
                     /locus_tag="JN692_003659"
                     /pseudo
     CDS             2281..2403
                     /locus_tag="JN692_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2665..3231)
                     /locus_tag="JN692_003660"
     CDS             complement(2665..3231)
                     /locus_tag="JN692_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265849.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(3513..3827)
                     /locus_tag="JN692_003661"
     CDS             complement(3513..3827)
                     /locus_tag="JN692_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265850.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3905..4015)
                     /locus_tag="JN692_003662"
                     /pseudo
     CDS             complement(3905..4015)
                     /locus_tag="JN692_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(4339..5016)
                     /gene="ospD1"
                     /locus_tag="JN692_003663"
     CDS             complement(4339..5016)
                     /gene="ospD1"
                     /locus_tag="JN692_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7265851.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMISNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(5387..5524)
                     /locus_tag="JN692_003664"
                     /pseudo
     CDS             complement(5387..5524)
                     /locus_tag="JN692_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5606..5904)
                     /locus_tag="JN692_003665"
                     /pseudo
     CDS             complement(5606..5904)
                     /locus_tag="JN692_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6493..6652)
                     /locus_tag="JN692_003666"
                     /pseudo
     CDS             complement(6493..6652)
                     /locus_tag="JN692_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6656..7012)
                     /locus_tag="JN692_003667"
                     /pseudo
     CDS             complement(6656..7012)
                     /locus_tag="JN692_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1860 a         1579 c         1477 g         2096 t
ORIGIN      
        1 ggttggctgt ttcaacatcg acagtgctcc ccatcgggcg caggcgagtc attagtggga
       61 tgtaaaaact gttacgggtg tactgcacca tccgttccac cttaccttta gtctgtgccc
      121 tgaaggggcg acacagtcgg ggagagaagc ccatctcctt gccgaactgc cacagcgaag
      181 gatggaaccg gtgctgaccg gtctgatatg cgtcacgttg cagaaccaca gttttcatat
      241 tgtcatacaa cacttcgcgc ggcacaccac caaagaagcg gaacgcatta cgatggcagg
      301 tctccagcgt gtcataacgc atattgtcag tgaattcgat gtacaacatt cggctgtatc
      361 cgggaacagc aacgaacacg tgaagcggtg agcgaccatt acgcatagtg ccccagtcaa
      421 cctgcatctg tcgtccgggt tcagtttcga accgaacggc aggctcctgc tcctgaggaa
      481 ccgagagaga acgaatgaat gccctgagaa tggtcattcc gccacgatat ccctggtctc
      541 tgatctcgcg agcgattacc gttgccggga ttttgtaagg atgagcatcg gcgatgcgtt
      601 gacgaatata atcccggtat tcatccagga gtgaagcaac agcaggtcgc ggcgtatatt
      661 ttggcggctc agattttgcc tgcaaataac gtttaacggt attgcgggag agccccagtt
      721 ctctggcaat cgcccggcta ctcattccct gcttgtgcag gattttaatt tccataactg
      781 tctcaaaagt gaccataagc tctcctgaat caggagagca gattaccccc tggatctgat
      841 ttcaggcgtt gggtgtggat cactattgca ccgttcgtga cagtgttgtt gcgccttgct
      901 gccaacgaag ctccactgcc catcaagttc acagataagg gcaacatcag cgtgagcgac
      961 tggcgcagat gttatccgcc ggggcgggag ctttttaatg tccgtatgac ggtattgatg
     1021 ccgattttca gcgtacgggc agtatcgcga actccggcac cgttgaaagc catttcagtg
     1081 atttggtttt taactcccgg tttacgggcc tcgtaacagt aagtgagctg aaacacccgg
     1141 tgacagtcac ggcaacggaa tcgatcatgt ccttttgggt tctgcccgtg acggtaaacc
     1201 tgaacggact ggcaacgggg gcaatgaaag ttaactgtga ccatgaaaga accttaaaag
     1261 cgggtattat aacccctaat tcaactaatt ggaggcattg tcatttttaa tctatggata
     1321 cacttaaaaa tcctctcacc ttacgttaaa aaccttctca tgtttagagt tttttattat
     1381 gtttatcata atgatatgac gatctgagtt tttaaaatca agaagaaaag ataagttttt
     1441 taaatgcaca gtgcctgtgg ataactttgc gtaaatccgg aggacagatc actctgaagc
     1501 aagacaacag catctgaaaa caatatgctc acgtttcaac cacagggcgt aagtgatcct
     1561 acccacgtaa tatggacaca ggcctaagcg aggttctggt tttcaaattg ttccggactg
     1621 aggccgccac accaactgtg ccgtcgccag cgattgtaat cacattcgat ataattaaac
     1681 actgttgccc gcattatttc ccggctgata aagtgttctc cgtggagaca ttccactttc
     1741 agcgaatgaa agaagctttc cacgcaggca ttatcgtagc agcagccctt tgcgctcata
     1801 ctgccacgca gattatgcca cttcagcagc gcctgataat ctgctgaaca gtactgactt
     1861 cctcggtccg agtgaacgat aacgttctgg ggtctcttac gccgccacag cgccatctgc
     1921 agggcatcgc aggccagttg cgccgtcagg cgtggtgaca tggaccagcc aataacggca
     1981 cgtgaccaca ggtcaatgac cacagccaga tacagctagc cttcatctgt acgtaagtac
     2041 gtgatgtctc ccgcccactt ctggttcgga ccactggcgt aaaaatcctg ctccagcaga
     2101 ttttctgaca caggcaggcc atgtgcgcgg taactgaccg tattgctggg taagatcaat
     2161 ttgctcactg gtatttacta cgtgcatcga atgagtgatc aagagccgta tgacgcgaga
     2221 gtgtcacgta cggttccgtg agcagccgga gagtgaaact ctctccggct gactcgactc
     2281 gcaggttccg acagcggtga cgaacatgca gcggtgttgt tctcgctgat cggcacatgc
     2341 cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg ttattgaaca tatccaggac
     2401 tgaccggcaa accgggtacg cgatctgttg ccttggaaag ttgatctgac ttctcagtaa
     2461 atatcaatat gattctgacg agccgcttac ctttgagcag agaaagggga gagtgatccc
     2521 acatccatga gctaggaaaa tacaaaaact ggctatacaa caattcaata tattaggact
     2581 tacttgcgag gagaaatata gcaccgctca ggcaattcct gggtatttac ttttcttagt
     2641 ttctttactc gggatgttct gtcatcagaa aggcgattct aaatttgtaa tatagtcact
     2701 catattccca cgtaatttct ttagatcaat atattgcaca tttttctcaa aaaactgatc
     2761 ggaaataaga tttgcaacat tgcttgttgt atcattttta acgcttctat tcattccttc
     2821 acaaagttcg tccattttct ttttgaaata actgcttcct gatacaagat gatttatgga
     2881 actttgtgca gcattgatgt ctagagaaat gcgataatct ttctcaacct gtttaaatat
     2941 ctgtttacgg agattatcgc cgatttttcg gctgtgttct gcacaaaact tatcaataca
     3001 ttggtttacc acccgggata cgtagacttt tttttcatca tttttccaat ctgtaatatt
     3061 ttcaccgatt tgttctgaaa cagagctcaa aatactagta tttatttttg ttgatgatat
     3121 ttttactatc cttttcccca ttgtacagcg gataatctca tccacctttc ctgaaaaata
     3181 tcttttgtga gataaactga ttccaaaatt attaaaagat gttccaagca ttataatcac
     3241 tttgtcatgt aaaatagtaa cattgtcatt attttagctg attcaaacaa ttctgtaaat
     3301 gctttgagtt ctttactccc ttggctcaat gatggaccgt ctggaatccc agagactaaa
     3361 ctctctgaga aagagagtat gaaaaaactt ggggcagtcc ctaaatgaag ggaccaacag
     3421 cggtaaaata accggcatca ttcataatga tgccctgtta gttgtagtta tgaagattta
     3481 cgtcattaaa attgctgttc gtagtgtcag tcctatggtc tggcatcagc tgaggatcgt
     3541 agccgataca tcactggctt cacgacattc gtgttgaggc tattcatgaa aactccacgc
     3601 tgaaaacgcc gttttgtata aacggtcacg gtatgcccgg agccactgtt gctgatgaat
     3661 tcgataaaac cttggcgttt cttgaagcta tcgttaatgc ggataatgaa acaacgattg
     3721 gtgagattcg ctcttttgcc gacgccctgg acgctgtccg gttcaatcgc aacaaaatca
     3781 accggcagct gagcaaaccc aaccttgcct ctctggcgct ggagcatgaa gtgatttggc
     3841 tgggacgtag ccgctgacag cttctataaa agtatgatcc tacccacgta atatgaacac
     3901 aggcttaagt gaggttctgg ttttcaaatt gttcaggact gagacctcca caccaactgt
     3961 gccgtcgtca gcgattgtaa tcgcacttga tataattaaa cactgtatat ttcatttgtg
     4021 gagtctccag tctgaggggg ctttatgcat tccgtattgg gcactatgat gccagaaatc
     4081 tgcaaggctc cacaccttcc tgtatttccc ttcctctatc acagtgcact ttacttaata
     4141 gggtgggatg tgttcattac atttattgtg ttttttatga ttgtttttgc gtccagtgtc
     4201 gttcgtcatg ggaaactggt tctatgggta gtatcgctca gtctagttaa ctgtttgttt
     4261 ctttttagaa attaagcaga tcacacaatt cggggagaga tccttctaag tgcaattctt
     4321 tgcagctatt tagcacactt aaaacaactc ttgtattttg tcagaaataa tattaaacat
     4381 gtcttgttca ttattgttaa gtctgtctgc tatttctgta agttttatcc cactatgatg
     4441 gcatatattt atatatgcca tcatagcctc gatattccct ttccctgcgg ccagaaataa
     4501 tcctggtaac ccctcattat ctcttacatc caatagttta atcgtttctt ttggtgataa
     4561 atcaaaagtt ttaataaaat ctccataagc ttgaatcgca gcggaatgac cttcttgaag
     4621 agctatgaac aaagcagatt ccccagcatt atttcgtgct gcaagaactt ctagtttttc
     4681 ctctttgggt atttcggctg ccaacagcga ttcaccgtat ttacgcgctg catcggcatt
     4741 tccatgccaa atcaatgaaa acaaattatg gcggttatac tccttattat tctttgtcgg
     4801 aatggatatg ctctgtattt tggccaattt tctgaaagaa acaccactat atcccccccc
     4861 tgatttttct tcattgatgg cgtgggatat agcggtattt gtcacgttaa tgatattact
     4921 tatcattcct ttattttcat tagcagtatt gctgcctatt attaggtgca tatttttgtt
     4981 gtttgctgga tgtaatccat agttatttat tgacattttt gttcatcctt tttgctaaaa
     5041 aaaacaataa cctatttttg taatgtaata gatattagtc tattgctaat ctaaaaaaac
     5101 cattgccaca ttgaatggct atattcacca ataaattctt tcgatttcgt atattgacat
     5161 atcgtagata tatcttatcc ctaagattca ataatcgagt ccgtttacca ttctgtgcaa
     5221 gcgccttcac tcagaagtga ccgtcaaggc gatcaccaaa cttgataata aagcagctca
     5281 ttgccatacg ccagtcctta aggggcgttg tccatttctg tgatgctgcc tagatttaac
     5341 tgccagctcc ggcttatcta catctaaata tgtgagccta ataccgctac actttggcca
     5401 gactatgttt ttctccggga agtgggctaa ccgtctccat aaagtgacga acttttttca
     5461 acagtggcca tattgaacgg cactgatgat tacgggttat tgtctcatga agagccggcc
     5521 acatcactga cagcagtgac ttcaggcctt cagcaccaga cagcctgaac cagttaatcc
     5581 atcgcccgac agatttagta atctagggct ggtttttctt cagctgttgc ggttcgaaag
     5641 tgccattacg atcacgcaga gtacggattt ccagaggttc atctccagta gtgataatct
     5701 tcgttaagca accattgcgg gaattgctgc cagatctaag ctgatttttc tcgcacccaa
     5761 gatgatgagt catattagca ttgagtacgg tttcattttc agcatccaat caaactgact
     5821 gagatcttca ggatttttga gacttttggc tagttcatta gctaaagccc gcaacttatt
     5881 agatgtcaac tgtttttcat tcataaattc accttcattt gatatcaaat taaatagttc
     5941 aacatcagag aattatacaa acttatgtac aggctcctat gatcttcgaa tcctgaagat
     6001 tatagtgaaa ataagttttc acgttgaaat cccacatagt tagtatatga taaataatca
     6061 tggatgacct gattaacctc ttggggtagt gcttttatag aagaataaat attctactaa
     6121 tttaatacat cacctaaaat agtattgttt tttatggttg gttgaaggtc gtggtgatga
     6181 tgttagtcct tttgagaata ttccagtgcc agatcttact tataataaag gggcttggca
     6241 gtttggataa actattgttc ttattccact aatacaaact ttttgtaaag tatattaaat
     6301 atacttcatt tcatactgaa atccccggaa gtattcaatc aaacaattac accataaaag
     6361 gaagaggggg ccctaattaa gttgtcaagc atgtttatga tacctgcagg gtataaattt
     6421 tctggctgca agcagacaca tataaaacgt ctttattgat cctacccaca taatatggac
     6481 acagtcgtaa gtgaggctct ggttttcaaa ttgttccgga ctgagaccac cacaccaacg
     6541 gtgccgtcgc cagcgattgt aatcgcactc gatataacta aacactgttg cccgcactat
     6601 ttcccgatgg ataatagtgt tctccgtgga gacattccat tttcagcgaa tgaattttca
     6661 tctgttgctg tatccagtca tacagtgact gcatcagtgg cgcggctctg gcttttcttg
     6721 ccgccagacg ctgttctgct gaacatcccc ggacttctgc ctcgatggca tacagttcac
     6781 cgatacgctg cagggcttcc gtggtgatgt cggttggcac tcttgcatgc acatcgtgga
     6841 tttttctccg gacatgagcc atacatgcgg cttccgttat tctgccggat tcgtacaacg
     6901 cccggtaacc accgtaaaca tcggcctgaa gcactccgct gtaaccggcc agatgattct
     6961 gtggatggat acctttccgg tccggtgagt acgcgaacca gaccgccgga gg
//
LOCUS       DADLSE010000181         6912 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLSE010000181 DADLSE010000000
VERSION     DADLSE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.182"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN692_003668"
     CDS             complement(<1..207)
                     /locus_tag="JN692_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265852.1"
                     /translation="MNMLRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQS"
     gene            727..1422
                     /gene="minC"
                     /locus_tag="JN692_003669"
     CDS             727..1422
                     /gene="minC"
                     /locus_tag="JN692_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7265853.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1446..2258
                     /gene="minD"
                     /locus_tag="JN692_003670"
     CDS             1446..2258
                     /gene="minD"
                     /locus_tag="JN692_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7265854.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2262..2528
                     /gene="minE"
                     /locus_tag="JN692_003671"
     CDS             2262..2528
                     /gene="minE"
                     /locus_tag="JN692_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7265855.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2900..3244)
                     /locus_tag="JN692_003672"
                     /pseudo
     CDS             complement(2900..3244)
                     /locus_tag="JN692_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3279..3398)
                     /locus_tag="JN692_003673"
     CDS             complement(3279..3398)
                     /locus_tag="JN692_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7265856.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3359..3544)
                     /locus_tag="JN692_003674"
     CDS             complement(3359..3544)
                     /locus_tag="JN692_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265857.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3645..3818
                     /locus_tag="JN692_003675"
     CDS             3645..3818
                     /locus_tag="JN692_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265858.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3880..4164
                     /locus_tag="JN692_003676"
     CDS             3880..4164
                     /locus_tag="JN692_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7265859.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4168..4503
                     /locus_tag="JN692_003677"
     CDS             4168..4503
                     /locus_tag="JN692_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7265860.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4560..6881)
                     /locus_tag="JN692_003678"
     CDS             complement(4560..6881)
                     /locus_tag="JN692_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7265861.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1868 a         1641 c         1567 g         1836 t
ORIGIN      
        1 tgattgtaag ttttcagcgg ctttttcgcc gagatatttc cttgttaact ctggggaaat
       61 gccatctgag ttcacacaaa tatacgggtc gcaaacaacg ccgcacgccg gagattgaag
      121 tccgccagcg tcatgtccag acgccattga tgagaacatc atccccacac ctgacaatcc
      181 gatataaata atacgcaaca tattcatagc acatcctgat aatccaaccg tacaacactt
      241 atgctcattt cgtcatcaat ccgtaatcaa aaccgctgac ctggacgagt tacgatgacc
      301 tgacagaagt atcagacatc ttctgcggtt tctatgatag agcaattcta tggcggatat
      361 taagataacg ggtatctgtt ctttaatgat gctgcaaaaa atggaaagtt aagaaagcct
      421 ggcgtggtga aaaaatacac ttgcctcaat actggatatt taaacaggtc tggagatgct
      481 gtcacgatta gggtgcggaa aaaggaagcg taaataagaa gaatcaaaag tacgacggca
      541 atgggttgat tgacaagggt attttttaag ctatgaatca gcgccattta tcacagaata
      601 gacttttact ctgaataaat gggagggtga cttgcctcaa tataatccag actataacat
      661 gccttatagt cttcggaaca tcatcgcgcg ctggcgatga ttaatagcta attgagtaag
      721 gccaggatgt caaacacgcc aatcgagctt aaaggcagta gcttcacttt atctgtggtt
      781 catctgcatg aggcagaacc taaggttatc catcaggcgc tggaagacaa aatcgctcag
      841 gcccccacat ttttaaaaca tgcccccgtt gtactcaacg tcagtgcact ggaagacccg
      901 gtaaactggt cagcgatgca taaggcggtt tcggcaaccg gtttgcgggt tattggcgta
      961 agtggctgca aagatgcgca acttaaagcc gaaattgaaa aaatggggct gcctatcctg
     1021 acggaaggaa aagaaaaagc gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat
     1081 acaatgccgg tcacaaaaac gcgtttaata gataccccgg tgcgttccgg tcagcgtatt
     1141 tatgctccac aatgtgatct gattgttaca agccacgtta gcgctggggc cgaattgatt
     1201 gccgatggga acattcatgt ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt
     1261 ggtgaccggg aaacgcaaat attttgtacg aacctgatgg cggaattggt gtccatcgca
     1321 ggtgaatact ggctgagtga tcaaatccca gcagaatttt atggcaaagc ggcgcgactg
     1381 cagttagtcg aaaacgcttt gaccgttcaa ccgttaaatt gatccctttt tgacaaggaa
     1441 tttctatggc acgcattatt gttgttactt cgggcaaagg gggtgttggt aagacaacct
     1501 ccagcgcggc catcgccact ggtttggccc agaagggaaa gaaaactgtc gtgatagatt
     1561 ttgatatcgg cctgcgtaat ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg
     1621 atttcgtcaa cgtcattcag ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc
     1681 gtactgaaaa tctctatatt cttccggcat cgcaaacacg cgataaagat gccctcaccc
     1741 gtgaaggggt cgccaaagtt cttgatgatc tgaaagcgat ggattttgaa tttatcgttt
     1801 gtgactcccc ggcagggatt gaaaccggtg cgttaatggc actctatttt gcagacgaag
     1861 ccattattac caccaacccg gaagtctcct cagtacgcga ctctgaccgt attttaggca
     1921 ttctggcgtc aaaatcacgc cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc
     1981 tgttaacgcg ctataaccca ggccgcgtaa gcagaggtga catgctgagc atggaagatg
     2041 tgctggagat cctgcgcatc aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc
     2101 gcgcctctaa ccagggtgaa ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct
     2161 acgcagatac cgtagaacgt ctgttgggag aagaacgtcc tttccgcttc attgaagaag
     2221 agaagaaagg cttcctcaaa cgcttgttcg gaggataagt tatggcatta ctcgatttct
     2281 ttctctcgcg gaagaagaac acagccaaca ttgcaaaaga acggctgcag attattgttg
     2341 ctgaacgccg tcgcagcgat gcagaaccgc attatctgcc gcagttgcgt aaagatattc
     2401 ttgaggtcat ttgtaaatac gtacaaattg atcctgagat ggtaaccgta cagcttgagc
     2461 aaaaagatgg cgatatttct attcttgagc tgaacgtgac cttaccggaa gcagaagagc
     2521 tgaaataagc ccgctgtaaa aacgcattta tcttcaaggc agaatcattt ctgccttgag
     2581 tttttcatcc ctctcatcca cgttgtggta aagcggcgag tattcttgct gatactcctc
     2641 attgctattt ccacctcccc ctttacacct taaggctgtt cccacgcaca gaacaaaatg
     2701 tttatgcctg gttgagtaaa taaccatgtt gttagtgtgg atatactggg agatgataac
     2761 cccgcaattt cattattcat tgttctccca taggatgagg tttcccgccg taactggcgg
     2821 gctttttttg ccccagaatt ttctctttca acatcctgta aacgaaaact gcgccgaagc
     2881 gcagtttaat cgttgatggt cagaacgacc agttcacacc agccaccgcg ttccacgggg
     2941 actccacacc ggcaccatgg ctatacccca ccccgaaatg cccgcttaac gtactgctga
     3001 atgaggcttt aatacctgcc tggtatattc cacgtctgcc cgacaaatca ttgacaaaat
     3061 taccgtcact attcactttc acccgattat catcgacaaa ttctttgcgc acagccgcct
     3121 tcaggcaggg ttcaatttcc ataccgttcc ccagacgcat gttgtaactc agcgttgcac
     3181 ccagttcacg atatatactg cgggtatcga ctgatttcga ttccatgcca ttggataaat
     3241 gatagtagcg tctaagtgac ctaagctctg ctgcaacatc agcccgcagg caccagacca
     3301 ggggattcat cctgaagaga cagcgcaggt gtattgtgtt caccgctcac caaagacatc
     3361 atgatgaaat gatgatatcc cgcataagaa agaggcattt tttacacgca gtgcgctgaa
     3421 gtgtagttgg ataaaaaatc actcttttca gataatactg tcgtacgctt atctttagac
     3481 aatgaggccg tcggcaaaat aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc
     3541 tcacataaaa atcacacaat aatattgata aatcagatta tcttaccttt acaacattgc
     3601 gttaacagtc aatgacactt cttgaataaa aatattgtca atcaatgagt tattcctcat
     3661 ttaagattat tctcattcac gtaaaaaaca taattcctat catcacagcc acgctcattt
     3721 tgaattatct gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
     3781 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
     3841 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaaccgcacc
     3901 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
     3961 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
     4021 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
     4081 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
     4141 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
     4201 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
     4261 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
     4321 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga tttaaaaatt
     4381 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
     4441 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
     4501 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
     4561 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
     4621 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
     4681 ttaatttccc ccaaattact caccccatct tgttcgactt tcacaccgtt cattgagaca
     4741 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     4801 tgcggaccgc tgtttttctc ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg
     4861 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttccgtgtg cgcattatga
     4921 tcgacgccca tccagatagc ctgtgcctgt ggctggaaga tccacgtata tttcgccgct
     4981 tccagaccaa aggatttatt cagggcaaaa ccgtaacccg cttccagcga gacggttgca
     5041 cctttcgcgc tataggattc ataggctaat tcatcccctt tcacggagtt tttaaaccag
     5101 ctatattgcg cccaggagtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     5161 gtggcataca gccctgcgct atagcctctg acactccctt tcgaacgata atccgacacg
     5221 ctggaatgag ttaagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     5281 tcactatcgg taaaaccacc ggttaacaga tcgcccccta actgcgagac gtagcgatgc
     5341 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     5401 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg cgtttcaccc
     5461 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     5521 cttccagctt cggggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     5581 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     5641 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     5701 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     5761 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     5821 ttaataacga ctttatccgt agctgaatta tcatcatcca attcagttct gagatgcaat
     5881 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     5941 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     6001 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     6061 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     6121 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     6181 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     6241 gataaaatat ctaccgttgc ttcctgacca tttatggaag actccagcac aattccagac
     6301 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     6361 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     6421 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     6481 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     6541 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     6601 gctatggcat tattttcatt acttcctgta tcggcagtac tttcttcatc aagagaagat
     6661 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     6721 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     6781 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     6841 gagtaatccc ccgttgtgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     6901 tcacttacga tt
//
LOCUS       DADLSE010000182         6884 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLSE010000182 DADLSE010000000
VERSION     DADLSE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.183"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN692_003679"
     CDS             complement(102..1316)
                     /gene="rspA"
                     /locus_tag="JN692_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7265862.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1521..1847)
                     /locus_tag="JN692_003680"
     CDS             complement(1521..1847)
                     /locus_tag="JN692_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HAY7265863.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1982..2323
                     /locus_tag="JN692_003681"
     CDS             1982..2323
                     /locus_tag="JN692_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7265864.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2389..2918
                     /gene="speG"
                     /locus_tag="JN692_003682"
                     /pseudo
     CDS             2389..2918
                     /gene="speG"
                     /locus_tag="JN692_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2921..3631)
                     /locus_tag="JN692_003683"
     CDS             complement(2921..3631)
                     /locus_tag="JN692_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7265865.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3739..4044
                     /locus_tag="JN692_003684"
     CDS             3739..4044
                     /locus_tag="JN692_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7265866.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4243..5700
                     /locus_tag="JN692_003685"
     CDS             4243..5700
                     /locus_tag="JN692_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7265867.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWLETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     LRDGVRGKDKLDVPIKFIWNFKDLKRTLSRPEVLH"
     gene            complement(6371..>6884)
                     /locus_tag="JN692_003686"
     CDS             complement(6371..>6884)
                     /locus_tag="JN692_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265868.1"
                     /translation="VDVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTEPSFGSE
                     VSDYGIIAHESQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTA
                     LSFGCFRHDGENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVV
                     KRLKFTKTEA"
BASE COUNT         1829 a         1763 c         1622 g         1670 t
ORIGIN      
        1 cttcgggata acgcttaccg ctcatgggca cctctcttta agccatctta aatgactctg
       61 aggtgtctgt taaacccgtg gcgattcaat tatcttactc cttacctgtt ccacagcgtg
      121 ccatcttcca gacgtgcgac tggtagataa gcaggttcat agggatattt cgccgccagc
      181 ttttcatcga attcgatacc aagacccggt ttgtctcccg gatgcatata gccgttatcg
      241 aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat gtattcctgg
      301 acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca gactggtgac
      361 aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa atcggcaatc
      421 cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata atcgatgagt
      481 tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc cactgcgatg
      541 ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc tggcgtcggg
      601 tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc cgcttcaata
      661 ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa cttgttacgt
      721 accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca cagctgctct
      781 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
      841 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      901 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      961 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
     1021 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
     1081 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
     1141 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
     1201 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
     1261 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
     1321 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcca ctgccataca
     1381 agtatgaaga tcgaaaaagc cggagtgatc acaaaaaaag gcgtatattt gcgctgtgaa
     1441 tggttgacaa aagatgaaat agaatacctt ttgtcagctg acacttcctc gtatcttatt
     1501 gataaaatgg atttatgttc ctacgtgcgc ccccagcccg caacaatgat caacattccg
     1561 caaagcgcaa tcaacgcacc cgtccagtca taaagactta gtttcacgcc atccacaacg
     1621 cgcagccaca tcaatgccgt gcagacataa acgccaccat aagccgcgta aacacgccca
     1681 ctcgccgcag gatgcaacgt taacaaccag acaaacagcg ccagtgaaat ccccgccgga
     1741 agcaacagcc agatactggc gtttcgtttt aaccacaacc agggcagaaa gcatccaata
     1801 atttcacaca gcgcagtagc aaaaaatagt aacgttgttt taatcatctt tgtcacttat
     1861 tgacatcatg tatagttata gggcgacata atatcatcaa tataaacatc ctcctggtac
     1921 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
     1981 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     2041 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     2101 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     2161 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     2221 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     2281 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     2341 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     2401 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     2461 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     2521 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     2581 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     2641 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     2701 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     2761 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     2821 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     2881 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     2941 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     3001 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     3061 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     3121 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     3181 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     3241 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     3301 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     3361 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     3421 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     3481 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     3541 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     3601 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     3661 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     3721 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     3781 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     3841 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     3901 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     3961 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     4021 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     4081 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     4141 taataattct taacccaaaa tgggtaggct ccctctattg ttagcgcgct aaatattcaa
     4201 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     4261 atggtgggca tcagccgcag aacactcgtt aaatcaaccg caataggttc tctggcgctg
     4321 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     4381 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     4441 cgtctacatg ttaaagataa tgaagtgacc tggttggaaa ctgacaatac cggcagcgat
     4501 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     4561 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg ggaaacgtgg tgaaggcaaa
     4621 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     4681 gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat tgttggcggc
     4741 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     4801 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     4861 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     4921 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     4981 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     5041 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     5101 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     5161 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     5221 tataaagcct atattcttgg tgaaggtgac gataaaacag cgaaaacgcc gcagtgggct
     5281 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     5341 acaaaacccg cttatatctg ccagggctgg gggccgcaac gccaggcaaa cggcgagctg
     5401 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     5461 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     5521 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     5581 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     5641 ttcatctgga acttcaagga cttgaagcgg acccttagta ggcctgaggt cctgcattga
     5701 gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga tgtctagacg
     5761 gctggtcagg acccccggtg gcctcggtct ggacttgagg tgggcctatg gggggaagtc
     5821 tgctggctta atgtattaga tggtcattca catttttctg tcagaaacca tcggtcccac
     5881 tgtgagtcca aacctaagga cctagaagtc cacccacagc cttagacaga gtgtcgaaca
     5941 catccacacc gagtcgacca gagaggacca tgtagagacc cacagagagg aacacgagtt
     6001 tatagaccag agtcaggagg gacttcaggt ctgcggaagt catagacata aagcccctat
     6061 ctattatgtt atattattac attaacaaca gatccagacc attggtccag accatagtta
     6121 gttaatcaca gtgatagccc accacctgaa gtcctgctga tattcagtcc agacttacct
     6181 ctgctcatgg tcttacctcc ctcggtctcc tgaccttggg tatcgactac agcttgctga
     6241 gcgtcatctg gaggagctac aggaggagct acaggaggcg ggaccgaagc cccaccttga
     6301 gtcccaccac cacctgtcag gcccttggcg atggacctga agcctttact aatgctctta
     6361 cccattggtc ttacgcttcg gtcttggtga acttcaggcg tttgactaca ggcaggacgg
     6421 cagtagctga gccagtgccc atcttgacgg tcgtagcagc ttcagcgaca acgaacactt
     6481 taccgaggat ttccaagtcg tgggactgag agtcgccatc acgtgaaagg acgaccgtaa
     6541 tattctcacc gtcgtgacgg aagcagccga agctcagggc cgtgaagcgt ttagccaaca
     6601 tgaacacacc tttgtcctca ccgttgtaat acgcgatgtc ggtacccagc atatccacga
     6661 tagcgtcttc accagagaag ccctgtttag cagccagacg ggacagtgct ttcagttggc
     6721 tctcatgggc gataataccg tagtcagaca cctcagaacc gaaagaaggc tcggtgttct
     6781 catacagagc gtccatgata gcgtctgcct tagcggtcag gctaccagtc aggacaggaa
     6841 gctctacggt cttcagcagc ttagaggatt ccagaacgtc gacc
//
LOCUS       DADLSE010000183         6769 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLSE010000183 DADLSE010000000
VERSION     DADLSE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.184"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN692_003687"
     CDS             complement(101..739)
                     /gene="rutR"
                     /locus_tag="JN692_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HAY7265869.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1027..2118
                     /gene="rutA"
                     /locus_tag="JN692_003688"
     CDS             1027..2118
                     /gene="rutA"
                     /locus_tag="JN692_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HAY7265870.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIIARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2118..2810
                     /gene="rutB"
                     /locus_tag="JN692_003689"
     CDS             2118..2810
                     /gene="rutB"
                     /locus_tag="JN692_003689"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HAY7265871.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2822..3208
                     /gene="rutC"
                     /locus_tag="JN692_003690"
     CDS             2822..3208
                     /gene="rutC"
                     /locus_tag="JN692_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HAY7265872.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            3216..4021
                     /gene="rutD"
                     /locus_tag="JN692_003691"
                     /pseudo
     CDS             3216..4021
                     /gene="rutD"
                     /locus_tag="JN692_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
     gene            4031..4621
                     /locus_tag="JN692_003692"
     CDS             4031..4621
                     /locus_tag="JN692_003692"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HAY7265873.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4632..5126
                     /gene="rutF"
                     /locus_tag="JN692_003693"
     CDS             4632..5126
                     /gene="rutF"
                     /locus_tag="JN692_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HAY7265874.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     SVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5147..6475
                     /gene="rutG"
                     /locus_tag="JN692_003694"
     CDS             5147..6475
                     /gene="rutG"
                     /locus_tag="JN692_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HAY7265875.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6557..6730)
                     /locus_tag="JN692_003695"
     CDS             complement(6557..6730)
                     /locus_tag="JN692_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7265876.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT         1436 a         1826 c         1859 g         1648 t
ORIGIN      
        1 ttcgctgagt gtgaataccg ctgccgctgg cggtaacgcc agtttaatga cgatcggtta
       61 aacaggtagc cggaggatgt tacaacctcc tccggcatct ttaacgtggt cgaatcccct
      121 caataataat ccgctgcacg ttttcaaccg tttgattgaa aaatacctca tcgcgcaacg
      181 tcgcgcctgt caccgcctcc acctgagggg cgaaatcggc gtaatgttga gtggaagccc
      241 aaatcataaa aatcaaatgc tgcggatcaa tcggcgcgag tttgccgctt ttgacccaac
      301 cggcaatcag cgccgatttc tcatcaatta atgccttcaa atcgcccgtc agttcatcca
      361 ttaacaacgg cgcgcctgcc agcatctcca tacagaacag gcgcgaagcc tgcggataat
      421 cgcgtgagac ttccagcttc agacggatgt actcttttat cgccgccagc ggggcgaaat
      481 cttcacgaaa cgcttttaac ggtgccagcc agatatcgag aatctgccgc agcacggcaa
      541 tatacagcgc ctcttttgac gggaagtaat acagcagatt ggtttttgaa acacccgcca
      601 actctgcgat ctgctccagc cttgtgccgt gaaaaccgaa ttgtgaaaaa gtgtccagtg
      661 ctgcgctaag aatcgctttt ttcttcgcgc ttactgcgcg cgaacgttta cccgttgttt
      721 tcactgcgcc ttgcgtcatg cgctctcccc tcttgtagat tggtaacagg atagcaaaac
      781 ccccgcccgg cctcgacctt ctgcactctc atcgcgcacg gatgcatgtt ttatgtgcaa
      841 ctgttttgac cgtttagtcc actttttacc agatatttta gataacacaa tgctaacaca
      901 ttaataacat tcacttttta aaaactggca tccgctttgc aaacaagcca atacgcggct
      961 gaaaaggata tgcaggatgc agcgccccgt ctgactttca ctttacgaga cgaagagagg
     1021 ttgatgatga aaattggcgt attcgtacct attggcaaca acggctggct catttcgacc
     1081 cacgcgccgc agtacatgcc gacctttgaa ctgaataaag ccattgtgca aaaagcggag
     1141 cattaccatt tcgatttcgc cctgtcgatg atcaaactgc gtggctttgg cggcaaaact
     1201 gagttctggg atcacaacct tgagtcgttc accttgatgg cgggtctggc agccgtgacc
     1261 tcgcgcattc agatttacgc cactgctgcc accttaacgt tacctccggc aatcatcgcc
     1321 cgtatggccg caaccatcga ctccatctct ggcgggcgtt ttggcgtcaa cctcgtgact
     1381 ggctggcaaa agcccgagta tgagcagatg ggtatctggc ctggcgatga ctatttctcc
     1441 cgtcgttacg actatctcac cgaatatgtt caggtgctgc gcgacctgtg gggcacgggg
     1501 aaaagcgatt ttaaaggcga ttttttcacc atgaatgatt gtcgcgtcag tccgcaaccg
     1561 agtgtcccca tgaaagtgat ctgcgccggg caaagcgacg ctggcatggc gttctccgcc
     1621 cagtatgccg atttcaactt ctgtttcggc aaaggcgtaa atacacccac ggctttcgcc
     1681 ccgaccgctg cgcggatgaa acaggccgca gagcaaaccg gacgcgacgt tggctcttat
     1741 gtgttgttta tggtgattgc cgatgaaacc gacgatgcca ctcgcgccaa atgggaacac
     1801 tacaaagcgg gcgcggatga agaggcgtta agctggctaa ccgaacaaag tcagaaagat
     1861 acccgctccg gtactgacac caacgttcgt cagatggccg atcccacttc ggcggtaaac
     1921 atcaatatgg ggacgttagt cggttcttac gccagtgtcg cgcgcatgtt agatgaagtc
     1981 gcaagcgtgc ctggtgcaga aggcgtgctg ttgaccttcg atgattttct gtcgggaatc
     2041 gaaaccttcg gcgagcgcat tcaaccactg atgcagtgcc gcgcccatct ctctgcgctg
     2101 actcaggagg tggcatgatg acaaccttaa ccgctcgacc ggaagccatt accttcgatc
     2161 cgcagcaaag tgcgcagatc gtggtggata tgcaaaacgc ttatgccacg ccaggcggct
     2221 acttagatct cgccgggttt gatgtctcaa ccactcgccc ggtcattgcc aacattcaaa
     2281 ccgctgtgac cgcagcgcga gcggcaggga tgttgatcat ctggtttcaa aatggctggg
     2341 atgaacagta tgtcgaggct ggcggccccg gctcaccgaa ttttcataaa tcgaacgccc
     2401 tgaaaaccat gcgtaagcag ccgcagctgc aggggaaatt gctggcgaaa ggctcctggg
     2461 attatcaact ggtggatgaa ctggtgccgc agcctggcga tattgtgctg ccgaagccgc
     2521 gctacagcgg tttcttcaat acgccgctgg acagcatttt gcgcagccgt ggaatacgcc
     2581 atctggtttt caccggtatc gctaccaacg tctgcgtcga atcgacgcta cgcgatggct
     2641 tttttctgga gtatttcggc gtggtgctgg aagacgcaac acaccaggcg gggccggaat
     2701 ttgcacagaa agccgcgttg ttcaatatcg aaaccttttt tggctgggtc agcgacgtcg
     2761 aaacgttctg cgacgcgctt tctcccacgt cctttgctcg tatcgcttaa ggagtttaac
     2821 gatgccaaaa tccgtaatta ttcccgctgg cagcagcgca ccgctggccc ccttcgttcc
     2881 cggcacgctg gctgatggcg tggtgtatgt ctccggtacg ctggcttttg atcaacataa
     2941 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
     3001 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
     3061 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
     3121 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
     3181 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
     3241 cttatgctga tgcacccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
     3301 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
     3361 gcaccggcaa taatcccgac acactggcag aagattacag tatcgcccag atggcagcgg
     3421 aactgcatca ggcgctggta gccgcaggga ttgagcgtta cgcggtggtc ggccatgcgc
     3481 tcggtgcgct ggtggggatg cagctggcgc tggattatcc cgcgtcggta actatgctgg
     3541 tcagcgttgc gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt
     3601 tcgcgaacga ttactgtata gcggcggcgc gcaagcatgg gtggaagcgc agccgttgtt
     3661 cctctatccc gccgactgga tggcggcccg cgcacctcgc ctggaggcag aagacgcgct
     3721 ggcactggcg cattttcagg gcaaaaataa tttactacgt cgacttaacg ccctcaaacg
     3781 cgctgacttt agtcaccatg cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag
     3841 tgatgatctg ctggtgccaa cagcatgttc cagtgaactt catgccgccc tgcccgatag
     3901 ccagaaaatg gtgatgccct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt
     3961 taatgctctg ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta
     4021 aggaattgct atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc
     4081 ccgcactcac aacggctggc gggagacacc cgtcagcgat gagacgttac aggagcttta
     4141 tgccctgatg aaatgggggc cgacatcagc taactgttct ccggcacgga tcgtgtttat
     4201 ccgcactgca gaaggaaaag aacgtctgcg cccggcgctt tccagcggca atctgcaaaa
     4261 aaccctgacc gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt
     4321 accactactg tttccccacg gtgatgcccg cagttggttt acctccagcc cacaacttgc
     4381 cgaagaaaca gcgtttcgca acagttccat gcaggcggcc tatctgatcg tcgcttgccg
     4441 tgcgctggga ctggataccg gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc
     4501 cttttttgcg ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgatag
     4561 cagcaaactt tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta
     4621 aggagcaaac tatgaacata gttgatcaac aaacctttcg cgatgcgatg tcctgtatgg
     4681 gcgcggcggt caatatcatc accacggacg gtccagccgg gcgcgccggg ttcaccgcca
     4741 gcgccgtctg cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg
     4801 cgtccgtctg gccggtattc aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg
     4861 ggcaggagcc gctttcaaat ctttttggcg gcaaaacgcc catggaacac cgctttgccg
     4921 ccgcccgctg gcagacgagt gtgaccggat gcccgcaact ggaagaggct ctggtttcgt
     4981 ttgactgccg tatcagccag gtggtgagcg tcggcaccca cgacattctg ttttgcgcca
     5041 tcgaagcgat tcatcgtcac accacaccct acgggctggt gtggttcgat cgcagttatc
     5101 acgcgctgat gcgccctgct tgttaacctc cttaaggaga cagctgatgg caatgttcgg
     5161 ttttcctcac tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga
     5221 acgattgccg tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg
     5281 cgcgacggtg ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc
     5341 ggggatcggc acgttactgt ttttctttat taccggcggg cgcgtcccca gttatctcgg
     5401 ctccagcgcg gcttttgtcg gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat
     5461 taacccgaat atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt
     5521 cattggcctg gtggtgatga aaattggtac acgctggatt gaacgactaa tgccgccggt
     5581 ggtgacgggt gcggtggtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaag
     5641 tgtatcggct tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct
     5701 ggtggcggtg tttacccgtg gcatgatcca gcggttattg attctcgtag gattaattgt
     5761 cgcctgtctg ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt
     5821 tactctcgtc agctatgctg cctggttcgg tctgccgcat ttttccactc ctggttttaa
     5881 tggccaggcg atgatgttga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg
     5941 tcatctcaag gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgagc
     6001 attcgtcggt gatggtctgg caacgatgct ttccggctct gtcggcggta gcggcgtcac
     6061 cacctatgct gaaaatatcg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt
     6121 tgttgcagct gccgtcatcg ctatgctgtt gggcttttcg ccgaaatttg gcgcgctgat
     6181 ccataccatt ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc
     6241 cgtcgcaggc gcaaggatat gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt
     6301 gattatggtc gcagtaacct tcgtgctggg cgcgggtgat tttgctctca cgcttggcgg
     6361 ttttacgttg ggagggatcg gtacagcaac ctttggcgcg attttactca atgcgttatt
     6421 aagcagaagg ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga
     6481 aaaccgccgg gttaccggcg gtgatgactt tttgacgagc ttaagaaaaa aatgtattcg
     6541 tcattgatta gcccggctag ttgtcgcttt tgccgtgact gctcttgcca ccttttttac
     6601 ctgcttcaga tgcgcgctgc ggatcattct tgaaattacc cccgctgtgc tgtccacctt
     6661 ttttacctgc ttctgatgct ctttcgcggt cttctgcaaa attgccggaa ccgcctcgat
     6721 ggtttgccat tactgacctc cgtctgggta tagacatcat attgcatag
//
LOCUS       DADLSE010000184         6713 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLSE010000184 DADLSE010000000
VERSION     DADLSE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.185"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            332..1987
                     /locus_tag="JN692_003696"
     CDS             332..1987
                     /locus_tag="JN692_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7265877.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            2127..2351
                     /locus_tag="JN692_003697"
     CDS             2127..2351
                     /locus_tag="JN692_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7265878.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            2414..2950
                     /gene="rimL"
                     /locus_tag="JN692_003698"
     CDS             2414..2950
                     /gene="rimL"
                     /locus_tag="JN692_003698"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7265879.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(2945..3925)
                     /locus_tag="JN692_003699"
     CDS             complement(2945..3925)
                     /locus_tag="JN692_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY7265880.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWLDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            4049..5041
                     /gene="tehA"
                     /locus_tag="JN692_003700"
     CDS             4049..5041
                     /gene="tehA"
                     /locus_tag="JN692_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7265881.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            5038..5631
                     /gene="tehB"
                     /locus_tag="JN692_003701"
     CDS             5038..5631
                     /gene="tehB"
                     /locus_tag="JN692_003701"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7265882.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            5934..6602
                     /locus_tag="JN692_003702"
     CDS             5934..6602
                     /locus_tag="JN692_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7265883.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(6620..>6713)
                     /locus_tag="JN692_003703"
     CDS             complement(6620..>6713)
                     /locus_tag="JN692_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7265884.1"
                     /translation="QQTPGQVENRALYPRPEGWGFTAHRIKSAI"
BASE COUNT         1718 a         1556 c         1642 g         1797 t
ORIGIN      
        1 ccagtcgcga agcatttgag caggcgttgg gttgtccggt tttggatgag ttccagcttt
       61 accaggaagc ggaagaacgc agtaaacgtc gctgtggttt gctttaaatt ctgaccatcc
      121 gcctttgcaa aaatttgcct gattttacaa acgaatcagg ctcatcccat cgacataaaa
      181 aaaatgccga tttatgcata ttctctcagt tcaacaattg gattattaat aaatattgtc
      241 tagagtgagc ggtcataaat aagcactttc ttgccgctga aaacgaccag cgcgggacca
      301 ttcacaacac cagaaggact cactttcagg tatggatcgt agacgattta ttaaaggttc
      361 aatggctatg gccgccgtgt gcggtaccag cggcattgct tctctttttt ctcaggcggc
      421 attcgcggca gattctgata ttgccgacgg gcaaacccag cgttttgact tctccattct
      481 acagtcaatg gcgcacgact tagcgcaaac agcgtggcgt ggtgcgcctc gtccgttacc
      541 tgacacgctg gcgacaatga cgccgcaggc ttataacagt attcaatacg acgccgaaaa
      601 atcgctctgg cataacgttg agaaccgtca actggacgct cagttcttcc atatgggaat
      661 gggattccgt cgccgcgttc gtatgttttc tgtagatcca gcaacacatc tggcgcgtga
      721 aattcacttt cgcccggagt tgttcaaata caacgatgca ggtgttgata ccaaacaatt
      781 agaagggcaa agcgatctcg gctttgccgg ttttcgcgtg tttaaagccc ccgaactggc
      841 gcgccgtgat gtagtatcat ttctcggcgc gagttatttc cgcgccgttg atgatacata
      901 tcaatacggt ttgtcggccc gcggcctggc gatcgacact tacaccgaca gtaaagaaga
      961 gttccccgac tttaccgcct tctggtttga tacggtaaaa ccgggggcaa ctacctttac
     1021 cgtttatgcg ttgctcgata gcgccagcat tactggtgcc tataagttca ctatccattg
     1081 tgagaaaagt caggtgatta tggatgtgga aaatcacctg tatgcgcgca aagacattaa
     1141 acagctgggc attgcgccga tgaccagtat gttcagctgc ggtactaatg aacgtcggat
     1201 gtgcgataca attcatccgc aaattcatga ctctgatcgt ctgtccatgt ggcggggcaa
     1261 tggcgagtgg atttgccgtc cgctgaataa tccgcaaaaa ttgcagttca atgcttacac
     1321 cgacaacaac ccgaaagggt ttggtttatt gcaactggat cgtgacttct cccattatca
     1381 ggacattatg ggctggtata acaaacgccc aagtctgtgg gtggaaccgc gtaacaagtg
     1441 gggtaagggc accatcggcc tgatggaaat cccaacaacg ggcgaaacgc tggataacat
     1501 tgtctgcttc tggcagccag aaaaagctgt aaaagcaggt gatgagtttg cattccagta
     1561 tcgtctgtac tggagtgcgc aaccgcctgt tcattgccca ttagcgcgcg ttatggcgac
     1621 gcgtaccggc atgggcggtt tcccggaagg ttgggcgcca ggtgaacact atcccgaaaa
     1681 atgggcgcgt cgttttgccg tcgatttcgt tggtggtgat ctgaaagctg ccgcgccaaa
     1741 aggcattgag ccggtgatta cgctttccag tggggaagcg aagcaaatcg aaattctcta
     1801 tattgaaccc atcgatggtt atcgtattca gtttgactgg tatccgactt cggactccac
     1861 tgatccggtc gatatgcgga tgtatctacg ttgtcagggg gacgctatca gtgaaacatg
     1921 gctgtatcag tatttcccgc cagcgccgga taaacgtcag tatgttgacg accgcgtgat
     1981 gagttaatcg ttttttcttc ggcaccttct tcgggaggtg ccgtctggtt aaacacgatc
     2041 ccgctcgcat ttttccctaa gttaaatgag taatctgatg gtgtgtattt cagatacacc
     2101 gtgtcagcca ctaacaggga gtgcgtatgt ttccagaata ccgagattta atatcccgtc
     2161 tgaaaaacga aaatcctcgc tttatgtcct tgttcgataa acacaataaa cttgatcatg
     2221 aaattgccag aaaggaaggt tccgacggtc gagggtacaa tgcggaagtg gtccgcatga
     2281 aaaaacaaaa gctacagtta aaagatgaga tgctcaaaat cctgcagcag gagagcgtca
     2341 aagaggtgta aactttccta agccgccagc caggcggctt ttttaacaac agcatggatt
     2401 gactggagat aagatgactg aaacgataaa agtaagcgaa tcacttgaat tacatgctgt
     2461 tgcggaaagt cacgtcacac ctctttatca gttaatctgt aaaaataaaa cctggttaca
     2521 gcagtcgcta aactggccgc agttcgttca gagtgaagag gacacgcgaa aaacggtgca
     2581 gggtaatgtg atgttgcatc aacgcggtta tgccaaaatg ttcatgattt tcaaagaaga
     2641 tgaacttatc ggcgttatct cgtttaatcg tattgaacca ctgaataaaa ctgctgaaat
     2701 cggctactgg ctggacgaat ctcatcaggg gcaggggatc atttctcagg cgctgcaggc
     2761 attgattcat cattacgctc agtctggtga acttagacgc ttcgtgatca aatgtcgggt
     2821 ggacaatccg caaagcaacc aggtcgcttt gcgcaatggt tttatccttg aaggttgcct
     2881 gaaacaggct gagttcctga atgatgccta tgatgatgtg aacttatacg cgcgtattat
     2941 cgattcataa taaccctgcc agcggcgttc gcgtaatgcg ctcctcgccg ttaatgactt
     3001 ttggaccacg gacatgaacc gtgtcgccat caaaggcttc aacgacggcg tggtcggtta
     3061 actccacatg gttttcaatt atcaccgcac ctgtaatacg gctctcacct tgtattacaa
     3121 cgtgctcgtc gagcagaatc ggtccaccac gtaccacggc atttccgcca atcaagacat
     3181 gatgttttaa cacacaatta ccttccacaa tggcatattc cgccacctgc gaactgtaat
     3241 gaatcgtcgg aatggcatct tcttctatgc cagctttcac ctgcgcgtga ccgtagactt
     3301 tagcgcaatc gcatagccag acgttgttct cttcattacc ttcaacactg gcaaaatcaa
     3361 aaacttcggc gcgatgttca ataaaagcat accggacgac ggcatcgcca taaatttgtg
     3421 cctggtggac aatacgcgag gcgctaaccc ttgcgcgatc ataaatttgg agcaggagtt
     3481 gatggtcggg cgttaagcct tgtgcggcga cgatcataga atgttgatca attaaggcgt
     3541 gaccaaatat tctacattgc ccgtaaacta atgagtcatg aatggtgaca ctgtcactta
     3601 tatatgcacc ctggctgatt tcactattat cgatccagac gttatccgtt gcgtaaacct
     3661 ctccccacag cacgctggtg ccggtgatac gcgtattacc ggaaatcact gttccgccga
     3721 acgcaatagc attctggtcg taaatccagc aatttccttc ctgtgctaac accgtttcgc
     3781 gatcgagcca gccgccagcg gttcctgcta tcacatcgtt aaaatcgcta atggcgataa
     3841 tctgccgtaa taacacactt tttttagtgc catcttcctg ataactaaag gctcgctgtt
     3901 cttcacttag gcgatatttg cgcatagcgt tttcccacag gtgacttact ataaccgtag
     3961 caaattctgc ggctctggct atgctcgaga aaattccata aaatgcattt caaatatact
     4021 ttataaatta aacaaaatga gtaagaagat gcagagcgat aaagtgctca atttgccggc
     4081 aggctacttt ggtattgtgt tggggacgat agggatggga tttgcctggc gctatgccag
     4141 ccaggtttgg caggtcagcc actggttagg ggatgggctg gtgattctgg cgatgatcat
     4201 ctggggatta ttgactagcg catttattac ccgactcata cgctttccgc atagcgtgct
     4261 ggcggaagtt cgccatccag tgctgagcag ttttgtgagt ttgtttccgg caacgacgat
     4321 gctggtggcg attggttttg ttccgtggtt tcgcccactg gcggtgtgca tgttcagttt
     4381 tggtgtcgtg gttcagttgg cttatgccgc ctggcaaact gcgggattat ggcgcggatc
     4441 tcaccctgaa gaagctacca cgcctggact gtatctgccg acagttgcca acaactttat
     4501 cagcgcaatg gcctgtggtg cgttgggcta caccgacgcc ggtctggtgt ttttaggcgc
     4561 aggcgttttc tcatggctaa gccttgaacc ggtgatcttg cagcgtctgc gcagttcggg
     4621 agaattaccc acggcactgc ggacatcact cggcattcag ctcgctcctg cgctggtggc
     4681 ctgtagtgcc tggctgagcg tcaacggcgg cgagggtgac acgctggcga aaatgctttt
     4741 cggttatgga ctgctgcaac tgctgtttat gctacgtctg atgccatggt atctctccca
     4801 gccatttaat gcttcattct ggagtttctc gttcggcgta tctgcactgg caaccaccgg
     4861 tttgcatctg gggagtggca gcgataatgg atttttccat acgctggcgg tgccgctgtt
     4921 tatctttacc aattttatta ttgcaatact gctcatccgt acttttgcgc ttctgatgca
     4981 gggaaaattg ttagtcagaa ccgagcgcgc cgttttaatg aaagcagagg acaaagaatg
     5041 atcattcgtg acgaaaacta ttttactgat aaatatgaat taacccgcac acactctgaa
     5101 gtactggaag cggtgaaagt ggttaaaccg ggtaaaacgc tggatctggg ctgtggcaat
     5161 ggtcgtaaca gtctttacct ggcagccaat ggttatgatg ttgacgcatg ggataaaaat
     5221 gccatgagta tcgccaacgt cgagcgcatt aaatccattg aaaatctgga taatttacac
     5281 acccgggtcg ttgatctgaa taacctcaca tttgatggac agtacgattt tattctttcg
     5341 actgtggtgc tgatgttcct tgaggctaaa accatcccag ggttgattgc caatatgcaa
     5401 cgttgcacta aacctggtgg ttacaacctg attgtggcgg cgatggatac cgctgattat
     5461 ccatgtaccg tcggcttcac gtttgccttc aaagagggag aattacgtcg atattacgaa
     5521 ggctgggaga gggtgaaata caatgaagac gtcggcgagc tgcaccgcac cgacgccaac
     5581 ggtaatcgta ttaaactgcg tttcgccacg atgctggcac gtaaaaaatg acccggtaag
     5641 cacaaaacgc gtgaaaattc cccacgctga gatgatttac tgttcttctt ttcggtaagc
     5701 atatttttta tcgaagggat gtgaaattaa tcacagtagt cgaagttttt tagcagctta
     5761 acttactgaa atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc
     5821 gtctgaaaag gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc
     5881 ggcgcttttt gctataactt agaaagtaat ataatcatct caggaaacta ttcatgcgta
     5941 ccacatcatt tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct
     6001 ctaaaatcac ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag
     6061 aaacaacctc agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa
     6121 gcaaatatga cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta
     6181 cccaggtagg gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag
     6241 aagcgatagc gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc
     6301 gtggggccat taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc
     6361 ctgttgcatt agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc
     6421 gcctctattt tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg
     6481 ttcgtcaggg cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata
     6541 ttaaacaagt tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt
     6601 agttccagac gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc
     6661 cttcagggcg gggatataag gcgcggtttt ccacctgacc aggtgtttgc tgt
//
LOCUS       DADLSE010000185         6641 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLSE010000185 DADLSE010000000
VERSION     DADLSE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.186"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..800)
                     /locus_tag="JN692_003704"
                     /pseudo
     CDS             complement(1..800)
                     /locus_tag="JN692_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(810..1601)
                     /locus_tag="JN692_003705"
     CDS             complement(810..1601)
                     /locus_tag="JN692_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HAY7265885.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1813..2460
                     /locus_tag="JN692_003706"
     CDS             1813..2460
                     /locus_tag="JN692_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7265886.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKVIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(2444..2989)
                     /locus_tag="JN692_003707"
     CDS             complement(2444..2989)
                     /locus_tag="JN692_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265887.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            3208..3828
                     /locus_tag="JN692_003708"
     CDS             3208..3828
                     /locus_tag="JN692_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7265888.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4136..5548
                     /gene="cycA"
                     /locus_tag="JN692_003709"
                     /gene_synonym="dagA"
     CDS             4136..5548
                     /gene="cycA"
                     /locus_tag="JN692_003709"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7265889.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN692_003710"
     CDS             complement(5593..6255)
                     /gene="ytfE"
                     /locus_tag="JN692_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7265890.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6363..>6641)
                     /locus_tag="JN692_003711"
     CDS             complement(6363..>6641)
                     /locus_tag="JN692_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HAY7265891.1"
                     /translation="PLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETL
                     AGLLYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
BASE COUNT         1538 a         1652 c         1731 g         1720 t
ORIGIN      
        1 ctgttaatat ctttctgcca ccagtaaggc gagaatattt ccagttcaat agcaccatta
       61 aatccggcgt tttcaaccag ccgacgaatt gacggaatat tgatgacgcc atctcccggc
      121 ataccacggt cattgaccag atcagtggtc ggcactaacc agtcggaaac atgaaacgcg
      181 aggatgcgtt ttccggcacg caggatctga ctggcgagat ccggatccca ccagacgtga
      241 taaacatcca ccgccacgcc gaggccaaac tcaccatcgg gatcgagttc atcacaccag
      301 tccagcgcct gacgtaacgt gcacaggcaa gagcgatcgg cagctgtcat cgggtgcagt
      361 ggctccagcg caattggcaa gccgacatct ttcgaatgtg gcagcaactg gcgaatccct
      421 tgtttcactt gttctcgcgc ttcatataga tctttagtgc ccgttggcag accgccaacc
      481 acctgcatat aacaggcggc gttaagcacc gctgcgtcgt ccagcgcccg gcggttatca
      541 tcaatcgcca gtttgcgttc cgccagtgtc ggcgcggtgt agtaagtgct acggcacaaa
      601 ccggaaacgc tcatgttact ggcggcaagc tggcgggtga tttgctggag atcttcaccc
      661 tacaactccc tgcgccaggg ggcaattgcg ccaataccgc gtgcggcgca ggcatcaata
      721 atggcgggta gcggcgtttt atagcccagt gtcgcggtat tgatactgaa taaatccggg
      781 cgttgtaaca gatctctcat gattcagcct cagaagcgcg gcatacttaa gccgccgtcg
      841 attgcgaccg cctgaccaca ggtgtaaatc agtttacctt ccgccatcgc gcggacggtg
      901 gaagcaatat cagccggata accccaacgt ccccacggca ccagaccttt ggcaatcagt
      961 tcgtcgtaat aagccgttgc cggaatagtc atatcagttt tgatgagacc agggcgcact
     1021 tcataaacgc caatttgttc attacaaagg cgagcagcaa acagacgagc agcggcagaa
     1081 acggcggttt tggcaatggt gtattcgccg cgattcatcg ccagcatgat ggcgttgatg
     1141 gaactgacga aaataatcga gcggtgcggc agctccgctt ccggtttcgg ctgtgccagt
     1201 aagcgcttac tgaatgcctg agcaaggaag aacggcgcgc gggtgttaat ggcaatgttt
     1261 tgatcgaagc tgtctggttc gaggtcgagg aggtcaccgc gttttttcac tgaaatcccc
     1321 gcgttattga gcagacagtc cagccgcccc cactggtttt gcgccgcgtc gagcatctcc
     1381 tcatgcagag atagatcacc gacatcagca gggaagcaaa tcacttccac gccttccgcc
     1441 aaacattctt gttgtgtgat atgcagtttt tctacgctgt cggcatcggg acgatcgtta
     1501 atcagcaggt taaacccgcc acgagcaagc tcctgcgcac agccctttcc aataccgcga
     1561 gcggctccgg taatcacggc aacaggacga gtggatttca tacaatagct ctcttttttg
     1621 aattttgttt ccttggtgat gattcgcttg cgactggagg ataaaaagac caaccatcta
     1681 gttggttgat atgcgatggc gatcacagga catttgataa cctgatgaaa gtacaagttt
     1741 ttgccctttt ttctgaagct gaaatcaaat caggttatga tagtcaccac gaagaggtcg
     1801 tggggaagcg ttatgtatat tgataccagc aacgtacaga gcctgaagga aaaattgctg
     1861 ctatgtgcgg ttaatgaatt tgccgaatat ggttatgaag gggccagagt agataatatt
     1921 gtaaaggcag cagggtgtag taaacagacg gtttatcacc actttggtaa taaagaaaat
     1981 ttatttatcg aagtgctgga atatacctgg aacgatattc gacaaaaaga aaaggccctt
     2041 gatttctcag atcttccgcc acaaaaagcc attgagaaag ttattgattt tacatgggat
     2101 tattatattg ctaatccttg gtttttaaaa attgtgcata gtgaaaatca aagtaaaggt
     2161 gtgcattatg caaaatcaca gcgtctctta gaaattaacc atgcgcactt gcagttaatg
     2221 gaatccttat tggatgaagg gaagaaatac aatattttta agccaggtat cgacccattg
     2281 caggtgaata ttaatattgc tgcgcttggg ggatattatt tgatcaacca gcatacgctt
     2341 ggcctggttt atcatatcag tatggtttcc ccccaggcac tggaagccag acgtaaggtc
     2401 atcaaagaga caatccttag ctggcttttg gttgaccctt catctaccgc gcacgaataa
     2461 aactgccatc cggctggcgg gtaaacagga cctgttgatt attccccgta tcaatggtta
     2521 agcccgtcac cacgccgctg gcgttctggc ggattttcac catctgccca ttttgcaaat
     2581 tactcagcgg cttacccgcg ccttctacct gcgccattgc atagacatcg gtagcgggca
     2641 gcccgtggtc gcggaacagc tgcgccatcg ttttaccggg ttcgacacgg taagagcgcc
     2701 attgattatc aattccgcta tctggctgga atggttgcgt ttgggtagtt tgcggctgtt
     2761 cttctgtcgg cggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg
     2821 gcgcgttggg cgtatcatca gatggaagca gaaatccgac caccaataca atggcggcaa
     2881 taatgatccc ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg
     2941 cgtgccagac tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct
     3001 gctccacgtc ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg
     3061 taaacccgcc tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt
     3121 tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta
     3181 cttttatacc ctaagaaagg aaatacgatg accaccccaa cttttgacac catcgaagcg
     3241 caagcaagct acggcattgg tttgcaggta gggcaacaac tgagtgaatc tggcctggaa
     3301 gggctgctgc cagaagcact ggttgcaggt attgccgatg cgctggaagg caaacatccg
     3361 gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa tccacgaacg cgccgatgcc
     3421 gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg tgaaatacct ggaagaaaac
     3481 gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc aattccgcgt gatcaaccag
     3541 ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg ttcattacac cggtaaactg
     3601 atcgacggca ccgtgtttga cagctccgtt gctcgtggtg aacccgctga attcccggtt
     3661 aatggcgtga tccctggctg gattgaagca ctgactctga tgccggtagg ttctaaatgg
     3721 gaactgacta tcccgcagga actggcatat ggcgagcgcg gcgcaggtgc atccatccct
     3781 ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa tcctctaagc agcgcattct
     3841 gttcccctcg aaccagaggg gagcaggcat tcagcaataa acccttcagt ttgccaaacg
     3901 gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt ttgtgagctg tttcgcgtta
     3961 tcaccgtgat atgacactca ctttaaacat aaaattaaca ttagatctaa atcttagtat
     4021 tcatcccgcg tattgttacc taatatcgat gagtcccgat acagattcgt cgtatcatag
     4081 actgactaaa ggccgtagag cctgaacaac acagacaggt acaggaagaa aaaacatggt
     4141 agatcaggta aaagtcgttg ccgatgatca ggctccggct gaacagtcgc tacggcgcaa
     4201 tctcacaaac cgacatattc agcttattgc cattggcggt gccattggta ctgggttgtt
     4261 tatggggtcc ggcaaaacga ttagccttgc cgggccgtcg atcattttcg tttatatgat
     4321 cattggtttt atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct
     4381 ggaatacaaa tcttttagtg acttcgcttc cgatttactg gggccgtggg cgggatattt
     4441 caccggctgg acttactggt tctgctgggt tgtaaccggt atggcagacg tggtggcgat
     4501 cacggcttat gctcagttct ggttccccga tctctccgac tgggtcgcct cgctggcggt
     4561 gatagtgctg ctgctgacgc tcaaccttgc caccgtgaaa atgttcggtg agatggagtt
     4621 ctggtttgcg atgatcaaaa tcgtcgccat cgtgtcgctg attgtcgtcg gcctggtcat
     4681 ggtggcgatg cactttcagt caccgaccgg tgtggaagcg tcattcgcgc atttgtggaa
     4741 tgacggcggc tggttcccga aaggtttaag tggcttcttt gccggattcc agatagcggt
     4801 tttcgctttc gtggggattg agctggtagg tacaacagct gcggaaacca aagatccgga
     4861 gaaatcactg ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt
     4921 cgcgctgatt gtgattatgt ccgtgacacc gtggagttcg gtagtcccgg agaaaagccc
     4981 gttcgttgag ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt
     5041 ggtgctgacc tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct
     5101 gtttggtctg gcgcaggaag gtgttgcgcc gaaagcgttc gctaaacttt ctaagcgcgc
     5161 agtacccgcg aaagggttga ccttctcgtg tatctgtctg ctcggcggcg tggtgatgtt
     5221 gtatgtgaat cctagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct
     5281 gtttatgttc gtctggacga ttatcctttg ctcgtacctg gtgtaccgca aacagcgtcc
     5341 tcatctgcat gaaaagtcga tctacaagat gccgctcggc aagctgatgt gctgggtatg
     5401 tatggcgttc tttgtgttcg tggtcgtgtt gctgacactg gaagatgaca ctcgccaggc
     5461 gctgctggtt accccgctgt ggtttatcgc gctggggttg ggctggctgt ttattggtaa
     5521 gaagcgggct gctgaactgc ggaaataacc gcattatcat gctggatggc gcaatgccat
     5581 ccagctttta gatcactcac ccgctagggc gcgtgggaac agtacattgt tttccagact
     5641 gatgtgatcc atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt
     5701 ggtgcaggct tctggcggcg gtgtgacgtt attggtagtg tgtttgatca cttccagcag
     5761 ttcgcccgct tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc
     5821 catgccttgt ttgatcatcg ggaagaggat ctgctcttct ttcatcatgt ggctggaaag
     5881 ctcttcatgc agcatggtca ggtattttgt cagccctttt ggcacgctcg gtttgtcggc
     5941 gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcggtgacg
     6001 atcgtggtag cgcacgatga tatggtcaat gatttctgcc agcggggcgc tacgccagtc
     6061 tttctcaatc ggttgttcag caagctttgc cagttcagct tcaatgacct caacatccag
     6121 ttctttacgt gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc
     6181 atatttacga aacagagccg aagcgcgagg gatagagagc gccagttcac ctaaaggttg
     6241 gtcgcgataa gccatagctg atacctcatt cttaataaga taagatgtat tttaaataca
     6301 cctttaaggc aaaagctata acccttacgt agcagaggct tatttaactc actgccagca
     6361 tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcgatg
     6421 accacgccaa tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg
     6481 cggagtaaaa aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga
     6541 atcactgtcg gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa
     6601 agcacggcta tcgcaaccat cagactaata aacaccagcg g
//
LOCUS       DADLSE010000186         6545 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLSE010000186 DADLSE010000000
VERSION     DADLSE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.187"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..231
                     /locus_tag="JN692_003712"
     CDS             <1..231
                     /locus_tag="JN692_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7265892.1"
                     /translation="IGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALK
                     MPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            320..1780
                     /locus_tag="JN692_003713"
     CDS             320..1780
                     /locus_tag="JN692_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7265893.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKTAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GNDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN692_003714"
     CDS             complement(1816..2019)
                     /gene="ydfZ"
                     /locus_tag="JN692_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7265894.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2197..2883)
                     /locus_tag="JN692_003715"
     CDS             complement(2197..2883)
                     /locus_tag="JN692_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7265895.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN692_003716"
     CDS             complement(2972..3718)
                     /gene="ydfG"
                     /locus_tag="JN692_003716"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7265896.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3855..5900
                     /gene="dcp"
                     /locus_tag="JN692_003717"
     CDS             3855..5900
                     /gene="dcp"
                     /locus_tag="JN692_003717"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7265897.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDEFASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5945..6463)
                     /locus_tag="JN692_003718"
     CDS             complement(5945..6463)
                     /locus_tag="JN692_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HAY7265898.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1624 a         1563 c         1667 g         1691 t
ORIGIN      
        1 attggtggtc ttatcgcttc cggttttggt cctatcctag cgggtatttt ctgcaccatg
       61 acggaatcct ggtatccgat cgccattatg atcatggcat attcagtgat tggtttaatc
      121 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
      181 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat
      241 cccttctctt accggagagg ggatttttat tcatataaca aaacatatag cttgccatat
      301 ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt
      361 ttatgatcgt aataacctgg ccccaagaat tgttcattta ggctttggtg catttcaccg
      421 tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata
      481 ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa
      541 tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt
      601 taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga
      661 accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc
      721 gaccggacag ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca
      781 gccgaaaaca gcaacagggg tgattgttga ggcgctggct cgccgtaaaa cggcaggact
      841 tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga
      901 cgttgtcact tcctacgcgc aagccgttga tgtaaaactg gcacaatgga tcgaagataa
      961 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
     1021 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgct ggcgttgcct gtgaaccttt
     1081 ccgccagtgg gttatcgaag ataactttgt agccggacgt ccggaatggg aaaaagcggg
     1141 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
     1201 cagtcattca ttcctggcat atctgggtta tcttgctgga tatcagcaca ttaatgactg
     1261 tatggaagat gaacattatc gtcatgcggc gtatggcttg atgttgcagg aacaagcgcc
     1321 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta
     1381 tagcaacccg gcgttacgtc atcgaacctg gcagattgcg atggatggca gccagaaatt
     1441 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
     1501 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
     1561 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
     1621 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtaatgattt
     1681 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
     1741 tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
     1801 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
     1861 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     1921 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     1981 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     2041 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     2101 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     2161 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     2221 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     2281 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     2341 taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt
     2401 cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag
     2461 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     2521 gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     2581 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2641 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2701 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2761 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2821 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2881 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2941 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     3001 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     3061 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     3121 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     3181 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3241 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3301 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3361 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3421 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3481 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3541 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3601 actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     3661 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3721 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3781 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3841 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3901 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3961 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     4021 acaatactat tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct
     4081 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     4141 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     4201 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4261 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4321 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4381 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4441 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4501 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4561 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4621 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4681 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4741 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4801 ttgcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4861 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4921 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4981 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     5041 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     5101 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     5161 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     5221 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5281 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5341 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5401 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5461 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5521 ttctcgatat gcgctggcat tgcttggaag aaaacgaagc aatgcaggat gtcgatgatt
     5581 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5641 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5701 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5761 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5821 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5881 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5941 ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata
     6001 ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct ttaatatgcc
     6061 acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc
     6121 cggtaatggc aatactgaca tcagcatccg cacgctctat ggcacctgtt gccatttctg
     6181 ctgccacttt ctcactcacc gcagaatatc gttcaagaga ctgctggctt acgctgagga
     6241 ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat
     6301 cttcagctgc acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta
     6361 agcgtaattg atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt
     6421 cggtatctgc gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct
     6481 attatatgta aaggtgattt gctttttctg tatgctcgtc caatataata attttcaaac
     6541 atata
//
LOCUS       DADLSE010000187         6508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLSE010000187 DADLSE010000000
VERSION     DADLSE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.188"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..476)
                     /locus_tag="JN692_003719"
     CDS             complement(<1..476)
                     /locus_tag="JN692_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HAY7265899.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYK"
     gene            665..2263
                     /locus_tag="JN692_003720"
     CDS             665..2263
                     /locus_tag="JN692_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7265900.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(2245..3003)
                     /locus_tag="JN692_003721"
     CDS             complement(2245..3003)
                     /locus_tag="JN692_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7265901.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN692_003722"
     CDS             complement(3020..3850)
                     /gene="glpG"
                     /locus_tag="JN692_003722"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7265902.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAGLARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN692_003723"
     CDS             complement(3895..4221)
                     /gene="glpE"
                     /locus_tag="JN692_003723"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7265903.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            4411..5916
                     /gene="glpD"
                     /locus_tag="JN692_003724"
     CDS             4411..5916
                     /gene="glpD"
                     /locus_tag="JN692_003724"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7265904.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(6185..6415)
                     /locus_tag="JN692_003725"
                     /pseudo
     CDS             complement(6185..6415)
                     /locus_tag="JN692_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         1578 a         1746 c         1678 g         1506 t
ORIGIN      
        1 tgtttatagt tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
       61 gcctccagct cagcccattt cgcatcgacg ttaaaaggcg ggttttccca tgcgccttta
      121 tcacgtttac aacgcccggt agtacgcccg tgtggtacgg ccgtttcaat cgcttgacgc
      181 agctccgcca ggttttcagg caggtctgcc gccgttaacg cggtacgcag cgcgttcatt
      241 ccacagccaa tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
      301 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
      361 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg
      421 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
      481 tttttctctt ttcgttgttt gcggtcctga taattgcaac cgtcgtgcca gaaaattaaa
      541 taagcagatt aattttttaa tttgttgttt ctaaaaagat tatatcttta tgtccgtaaa
      601 cggagatttc ccgcaaagcc gatttaccga taatgaaata tcgcctttta taaggatatc
      661 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
      721 cggcagtcag cgctggtcaa aatggcgtcc gtcactctgt atatgccagc aagaatcgtt
      781 ggtcatcgat cgactggaat tattgcacga tgcccgctcg cgctcgctat ttgaaacgct
      841 taaacgcgat atcgccagcg tttcgccaga aacagaagtg gtgggcgttg agattgaact
      901 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
      961 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
     1021 gcagatttgc tggtttctgc tggcggaagc gcgttatcta cccgcccggt tgatacaatc
     1081 ttcaactccg cgcaaaaaag aacagccgcg cggcccaggt gaagtgacga ttatcgatct
     1141 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacggc agcaaacgct
     1201 cgatttcctc aaatccggta ttgccacgcg taacccccac ttcaaccgca tgattgagca
     1261 gatcgaaaaa gtggcgatta aatcccgcgc gccgattctg cttaacggcc cgaccggcgc
     1321 aggtaagtca tttctggcgc gacgcatctt cgagttaaaa caggcacggc atcagtttag
     1381 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcga tgtcgacgct
     1441 gtttggtcat gtgaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gattattacg
     1501 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
     1561 ggcaatgctg ctgaaagcta ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
     1621 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
     1681 cgaaggcaaa ttccgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
     1741 gggtctacgc cagcgccagg aagatattga accgaacctg gattatgaag tggagcgcca
     1801 cgccacactc actggtgaca gcgtgcgttt taacaccgaa gcgcggcgtg cctggctggc
     1861 ctttgcaaca tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt
     1921 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg tggaagatga
     1981 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
     2041 gggcgcagag gcagaaaaca tcgatctctt cgaccgtatg caactggaac acgttatcgc
     2101 tatctgccgc caggcaaagt cgctttctgc tgccggacga cagctttttg atgtttcgcg
     2161 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
     2221 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatggt ggtccgtcag
     2281 cacctgcatc acgcttactg gcggcggggc gtcggtgtag acggcatcaa ccatgctgat
     2341 gctccccata ttgaccatcg cattacggcc aaattttgaa tggtcaacca ccagcataac
     2401 gtggcgcgag ttctcaataa tggcgcgctt ggtacgaact tcgtggtaat cgaactccag
     2461 cagcgagccg tcgctgtcga tgccgctgat ccccagaatg ccgaaatcaa ggcggaactg
     2521 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctac gtaattcgcc
     2581 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cgttgagatt
     2641 gttggtgaca atgcgcaaat tgctgtgatt gagcagcgcg tgcgccaccg cttccggcgt
     2701 ggtgccgata tcgataaaca gcgtcgagcc attggggatt tgctccgcca ctttgcgggc
     2761 gatgcgctct ttttcttcgg tctgggtggc tttacgatcg tgccacggcg tgttaaccga
     2821 actggaaggc agcgccgcgc cgccatgatg gcgcaggatc aggttttgct ctgccagctc
     2881 gttgaggtcg cggcgaatag tctgcgggct gacggagaag tgctccacca gttcctcggt
     2941 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
     3001 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
     3061 tcgctaaacc cacggctaat ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
     3121 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
     3181 aaatgccact ttgcggatcg cgttcgccac gtagccagac gtagcccatc agcgcataca
     3241 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
     3301 acagggcgct gataagcgta atgacaatta gcttaccgct accgaggcgt ttttccaccg
     3361 cgccgccgag ataccaccac caaagcaggt taaagaggat atgcatcagc gagaagtgca
     3421 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
     3481 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcaataaac accaccacgc
     3541 aggcgatcat catcacccag gtcaccggac ctgcgcgttc acgcaaggca gcaaagaaag
     3601 gataacggcg ataatgcagg ccactgccgg tatggcctgc cagccagctg gccgccagat
     3661 aacgcggatc tgccgggttt tcgagaaaac gcgccagccc cgcccgcacg cgctcggcct
     3721 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
     3781 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
     3841 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
     3901 gccgtacgcc acctctgcgg gaaactgtcg ctgccaggct tcaaagccgc cgtcaatgct
     3961 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcccctttgc tgctattgcc
     4021 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
     4081 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
     4141 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
     4201 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
     4261 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
     4321 gttttttcaa tgttacccaa agcgcgattc tttgctaata tgttcgataa cgaacattta
     4381 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
     4441 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
     4501 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
     4561 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
     4621 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
     4681 catcgtccgc atctgcgccc ggcgtggatg attcgcattg gtctgtttat gtacgatcat
     4741 ctgggtaaac gcaccagcct gccgggatca actggtttgc gttttggcgc aaattcagtg
     4801 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
     4861 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
     4921 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
     4981 accggcaaaa aatatagctg gcaagcgcgc ggcctagtta acgccaccgg cccgtgggtg
     5041 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
     5101 ggcagccata ttgtggtgcc gcgcgtgcat acccaaaagc aggcctacat tctgcaaaac
     5161 gaagataaac gcattgtgtt cgtgatcccg tggatggacg agttttccat catcggcacc
     5221 accgatgtgg aatacaaagg tgatccgaaa gcagtgaaga ttgaagagag tgaaatcaat
     5281 tacctgctga aagtgtataa cgcgcacttt aaaaagcagc taagccgtga cgatatcgtc
     5341 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaagccatt
     5401 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
     5461 ttcggcggta agctgaccac ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg
     5521 ccgtattatc agggtattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
     5581 attgaaggcg accgcgacga ttacgccgct cgcctgcgcc gccgttaccc gttcctgact
     5641 gaatcgctgg cacgtcacta cgcgcgcact tacggcagca acagcgagct gctgctcggc
     5701 aatgcgggaa cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggaa
     5761 ctgaaatacc tggtggatca cgaatgggtc cgccgcaccg acgatgccct gtggcgtcgc
     5821 acaaaacaag gcatgtggct gaatgcggat caacaatctc gtgtgagtca gtggctggtg
     5881 gaatatacgc agcagaagtt gtcgctggcg tcgtaaatta acgtaaggtg atcaggtcag
     5941 atttcaatct ggcctgagac tgatgacaaa cacaaaactg cctgatgcgc tacgcttatc
     6001 aggcctacgt ggtttatgca atatattgaa tttgcatggt cttgtaggcc agataagacg
     6061 ttcacgtcgc atccggcatg aacaaagcgc actttgtcaa aaatctaacc tactttttaa
     6121 ttcagggaat taccgcaaag cccacgccat catacccaac gtaacaaaac tcaggcacgt
     6181 tccccactcg ccccgagaaa aatagcatta atgcgcccag cgtcagcata aaaatattga
     6241 gcagaaggta aattgttggc gtaaagagta aattgcaaac ctcaggaata gcggcaagcc
     6301 cagcccccag catggcaata accgctgcaa aaaattgcgt cagtattggg atgccgaaaa
     6361 aagtcattac cagcgaggtc aaaatccatt ttctattttg cattattctt tccattcttt
     6421 ctgaatggcg aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat
     6481 gatggcgaaa aaaacgggac cctttggc
//
LOCUS       DADLSE010000188         6494 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLSE010000188 DADLSE010000000
VERSION     DADLSE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.189"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN692_003726"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN692_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7265905.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN692_003727"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN692_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7265906.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="JN692_003728"
     CDS             complement(2659..5772)
                     /locus_tag="JN692_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7265907.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5827..6054)
                     /locus_tag="JN692_003729"
                     /pseudo
     CDS             complement(5827..6054)
                     /locus_tag="JN692_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
     gene            complement(6279..>6494)
                     /locus_tag="JN692_003730"
     CDS             complement(6279..>6494)
                     /locus_tag="JN692_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265908.1"
                     /translation="RLSSILQHEDQEAFEEIFKTSCFMPKKGLKVDKVVPMGWTSGVA
                     HVKVYVKGNLYDLWTNTENLQSEKAGK"
BASE COUNT         1722 a         1513 c         1518 g         1741 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgatctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagcaaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctag ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atataaagcg
     6061 cgcaaaatta ttcaagatat ttttgaaaat cattcaagat aggctatcgc gtgtgatagc
     6121 aaaaacattc aagataaaaa tgcttgatta ttaagcaatc aggaatggga gtattaagtt
     6181 tcgtgtttcc agtaaaacca gagagtattg aaatagctat cacctaactt tatcaggcga
     6241 tagctggtag atgatgtgat tacaggttga ctactgcttt atttaccagc cttctcggac
     6301 tgaagatttt cggtgttagt ccaaagatcg taaagattgc ctttcacata aactttgaca
     6361 tgcgcaaccc cactggtcca ccccatagga accactttat caacttttag ccccttttta
     6421 ggcataaagc atgatgtttt aaagatctct tcaaatgctt cttgatcttc gtgttgcaaa
     6481 attgaactta atcg
//
LOCUS       DADLSE010000189         6468 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLSE010000189 DADLSE010000000
VERSION     DADLSE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.190"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..268)
                     /locus_tag="JN692_003731"
     CDS             complement(<1..268)
                     /locus_tag="JN692_003731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265909.1"
                     /translation="MKVQILNNNCEVVWSYDIAAHVDQSGDSWANGKHQIMAGVVFSL
                     RRALEQAEVFPSGPEWKWPFSICPNSESTFQKIGQKVALEEHQPT"
     gene            complement(462..1136)
                     /locus_tag="JN692_003732"
     CDS             complement(462..1136)
                     /locus_tag="JN692_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY7265910.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1227..1427
                     /locus_tag="JN692_003733"
     CDS             1227..1427
                     /locus_tag="JN692_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7265911.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            1471..2022
                     /locus_tag="JN692_003734"
     CDS             1471..2022
                     /locus_tag="JN692_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265912.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2019..2855
                     /locus_tag="JN692_003735"
     CDS             2019..2855
                     /locus_tag="JN692_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HAY7265913.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEGFMSMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKAGAGIGVLEMLSAIYDAPASFLSSASAHTQIMVGWT
                     GALKGAPVSDNAGYANPVQFTTSEIGVSGGEGNSLLSEAAIMATVPALTRLNDEDLHK
                     LSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRLAHKANFHIHRLLNISGGHR
                     HA"
     gene            2860..3084
                     /locus_tag="JN692_003736"
     CDS             2860..3084
                     /locus_tag="JN692_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265914.1"
                     /translation="MILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDRECQKVV
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            3081..3898
                     /locus_tag="JN692_003737"
                     /pseudo
     CDS             3081..3898
                     /locus_tag="JN692_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3895..4389
                     /locus_tag="JN692_003738"
     CDS             3895..4389
                     /locus_tag="JN692_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7265915.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMEAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            4389..5042
                     /locus_tag="JN692_003739"
                     /pseudo
     CDS             4389..5042
                     /locus_tag="JN692_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
     gene            5039..5365
                     /locus_tag="JN692_003740"
     CDS             5039..5365
                     /locus_tag="JN692_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7265916.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     KRGLKV"
     gene            5362..5751
                     /locus_tag="JN692_003741"
     CDS             5362..5751
                     /locus_tag="JN692_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7265917.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            5771..>6468
                     /locus_tag="JN692_003742"
     CDS             5771..>6468
                     /locus_tag="JN692_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7265918.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKG"
BASE COUNT         1623 a         1499 c         1744 g         1602 t
ORIGIN      
        1 cagttggctg atgctcttcg agtgcgactt tctgaccaat tttctgaaat gtactctccg
       61 aatttggaca aatagaaaaa ggccatttcc attcagggcc tgatggaaag acttcagcct
      121 gttctaaagc acggcgtaaa gagaatacaa ctccagccat aatctgatgt ttcccattgg
      181 cccagctatc gccgctctga tctacatggg cggctatgtc gtatgaccaa acgacttcac
      241 agttattgtt taaaatctgg actttcattt catacacctg ctttaacatg agtacctagt
      301 ggcacaacat gactcaacga atcatcctgg acttcatatg ccccaggcgg ctacttcgtg
      361 ggcgtcctgc ctgtttgttg tttctcttgg gtacattatg tatctcatgg gtacattgtc
      421 aagtataaaa aaacctgccg aagcaggttc ataaacattg attaggcttt gattttgtat
      481 cttcttggtt ttcctgagaa aatcacagtt ccaattatag agcaattacc gttgatctta
      541 atgtaaggct caggccagtt tgggtttaac gctttgagat aacgctgtgt cccatcttct
      601 atcaaccttt tgaaggtggt ttcacctgta tcgtgcatca atgcaataac gtcgtcaccg
      661 tggcaggcag gtacttcagg atcgacaaaa atcatgtctc ccgggcggta ctcatcaatc
      721 attgaatcac ctatcacccg caagatataa gtcatttccc cacagggtac agggcaggga
      781 tacgtttctg ctgtgctcaa atcaacctca gaatatccaa cttctttcca tgctccggcc
      841 tgtacccatg atatgacagg gactaatgtg atttgtttat tagtgattga aacatcaggt
      901 ttttttgtga tgttcgttgt ctggtgttct tgatcgagcc atccgacagg caggtcgaaa
      961 catttttcga tgtgccgtgc catgctgtca ccgatatttt tagtagcgcc atcccccata
     1021 aacctgctgg tttgggttgg ctcgcgatca atcatggtgg caaaggatga atttccgcca
     1081 acaccatctc tcagttttct ggcgttagac cgccggatgt catggattgt tttcataacg
     1141 aaattaaaac ctttgtaccg ataaggtaca agtatcttga gggttcattt caatcatgta
     1201 atatgtatac cggaggtaca tattgtatga aagcgtattg ggactcttta accaaagaac
     1261 agcagggcga gttggccgga aaagttggct caacacctgg ctacttacgg ctggttttca
     1321 atggttataa aaaagccagt tttgtgctgg cgaaaaaact tgagcaatgc acgtcaggtg
     1381 caattacgaa atctgactta agaccggata tctatccgaa agattaacag aacaccttca
     1441 atttttaacc acagaacgat gaggctaacc gtgggtaagt atcactggaa agtagaaaaa
     1501 cagcctgagt ggtacgtgaa agctgtcaga aaaactatcg cagcgttgcc gggtggttac
     1561 gctgaagcag ctgactggct ggatgtaaca gagaacgcat tatttaaccg ccttcgtgcc
     1621 gatggcgatc agattttccc gctgggatgg gcaatggttt tacagcgcgc ggctggcact
     1681 cactacattg cggatgctgt cgcacagtct gctggtgggg tgttcgtatc gcttcctgaa
     1741 attgaggaag tagagaacgc cgatataaac cagcgcctgc tggaagtcat cgaacagatc
     1801 gggaattact caaagcagat tcgttcggca atcgaagatg gggtcgtgga gccacacgag
     1861 cagacagcaa ttaatgatga gttgtatctg tcaatttcga agctccagga gcatgcagca
     1921 ctggtctaca aaatcttttg cgctccagaa aagagtgacg cccgcgagtg tgcagctccg
     1981 ggcgtcgtgg cgttttgtgt ctgtggagaa actaacgcat gaacagttta acggcaaata
     2041 accgtttgtc gcaacagctg gtggtcagtg tcgctgaaca cctgttgtta cggcatgaat
     2101 gcagattacc aaatcacctg gctgtaagta accacagaga actttacctg actgtggggg
     2161 gcgagttgtg caggaactta accgctggtt tcgtgacgga agagggcttt atgtccatgt
     2221 tattcgttgg gagccagaaa cacagcgcgt tatctatctt cgcaaagact acccgcatga
     2281 gtgctttagt cctttgtgga aattcaggcg tgattttgtt gagtgtgaag gaccaccagc
     2341 acattgattc tgccattccg ggacgttaca ctgttcaggc accttataaa gcgggtgccg
     2401 ggattggcgt cctggaaatg ttatcggcga tatatgacgc gccagcgtct tttttatcgt
     2461 ctgcgtctgc gcacacccaa attatggtgg gctggacggg ggcactgaaa ggtgcgccgg
     2521 tttccgataa cgccggttac gccaaccccg ttcagttcac caccagcgaa attggcgttt
     2581 ccggtggtga aggtaattca ctgttatcgg aggctgccat catggctact gtcccagccc
     2641 tcactcgtct gaatgatgaa gacttacata aactcagtta tgtaacaact gcactacgtg
     2701 ctctgcgcaa ggtaactctt tcggatccgc aggcgcatca ggttctggta gaaacccttc
     2761 ttaacttgca ggctgaacgt atccgtttgg cgcataaggc taattttcat attcaccgtc
     2821 tcctgaatat cagcggaggg catcgtcatg cttaatccgt tgatcctcaa tatttgccgt
     2881 ttgcttcagc gtaaaaaaac atcaattcct acagttgggc agtggtacac cacgcctgca
     2941 gggcatgttc tacgtgttag cctggttgac cgtgaatgtc agaaggtggt ttgtgaaccg
     3001 ctgggccgta attaccgcgt cagtatgccg cttatagcct ttcgctccgg aaaaaacatg
     3061 aagcatctcg gaggtgcagc atgagtatgg agctgatggt taaagcgatg aaaattcgag
     3121 tgggtaatcc attgcgaaaa ctggttctga tcaagctggc tgataatgcc agcgatcagg
     3181 gtgagtgctg gcccagctac cagcatattg ctgaccagtg cgagattagc aaacgttctg
     3241 tgatgaatca tattgcggcc ctttgtgagt ccgggctggt aaaaaaagtc acccggaaag
     3301 gtgaaaaagg taactcaagt aatatctatc tccttcatct tgatggtgca ggagattcac
     3361 taggggtagt gcaaataatt tactatctgg tgcagcaaat tcactaggta gtgcaggagt
     3421 tgcaccaggg ggtagtgcag gagattcacc cagaaccagt cactcttttg aaccagtcaa
     3481 agaaccagtc aatgacccaa tagctgttgg tgcatctgct gatgagtctg tgcgagttcg
     3541 ttcaaaccga ccggaatact ctccggagtt tgagcaggca tggctggcct atcccaaacg
     3601 tgctggtggc aattcaaaat ctgcagcctt caaagcctgg aaagcccgtt tgaatgaggg
     3661 ggtaaacccc gaaaccatgc tggaaggtgt gaaacgctac gcgggctggg tatctgcgat
     3721 gggtaacagc ggcacacaat ttgtgaaaca ggctgtcacg ttctttggtc cggatcgtca
     3781 tttcgaagaa tcctgggaag ttcctgcagt atctgcagcc agacgcgagg acccgtactt
     3841 caaagccagt tacgacaacg tggactacag ccagatcccg gcaggattca gggggtgatt
     3901 atgagtcttt tgaatgaagt tcagaaattc attgaagccc atccggggtg tacttccgga
     3961 gacattgcgg atgcttttgc tggttactca cggcagcgcg ttctgcagtc agcaagcaag
     4021 ttacgtcaga gtgggcgtgt ggctcaccgt tgtgaaggag atacacgcag acatttcccg
     4081 cgcctgactg agagagcgca ggagccggaa ccacaatctg ttcgtgaaac cagacctgtg
     4141 cgcaatttct atgtcggcac taacgacccc cgggtgattt tgtgcctgac ccgccaggct
     4201 gaagaactgg agtccagggg cttattccgt cgagctgcaa cggtgtggat ggaggcattc
     4261 cgtgaaagcc actcccagcc agaacgaaac aattttctgg cgcatcgtga gcggtgctta
     4321 cggaaaagca gcaagcgcgc tgcatcgggt gaagagtggt atctgtcagg gaattacgtg
     4381 ggggcttaat gagtaataaa tattgccagg cgctagtgga gctgcggaac aaaccagccc
     4441 atgaactgaa ggaagtgggc gatcagtggc gcacgccgga caacattttc ttgggaatta
     4501 acaccctgtt tggcccgttt gttctggatc tgtttactga tggtgataac gccaaatgtg
     4561 ccgcttatta cactgcggaa gacaacgcgc tggcgcatga ctggtcagaa cgtcttgcgg
     4621 agcttaaagg tgctgccttt ggtaatcccc cgtacagccg cgccagtcag catgaggggc
     4681 aatacatcac cggcatgcgt tacatcatga aacatgccag tgccatgcgt gataaaggtg
     4741 ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt ttggtggccg gaagatgcag
     4801 accatattgc ttttattcgc gggcgtattg gttttgaact gccagcctag tttatcccga
     4861 aggacgagaa gcaggtgccg acaggcgctt tcttcgctgg tgctattgct gttttcgaca
     4921 agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag gcatgtggtg
     4981 aggcctttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag atggcggcat
     5041 gacgacatta actcaatgcc agcagcaggt gctggatatg ctgatttctt accagaaaga
     5101 acgtggcttc ccgccaacca atcaggaggt ggcaaccatg ctgggatacc gttcagtgaa
     5161 tgcagcggtg gagcatcttc gcgcactgga gaaaaaaggc gtcatcacga taaagcgtgg
     5221 cgtggcccgg ggcatcacgc ttcataccgc ggtgaaggac gacgacagcg aggcggtcgg
     5281 gattatccgc tcactgcttg ccggtgagga aaacgccagg ctgcgtgcaa cccactggtt
     5341 acataagagg ggcctgaaag tatgaagctg atcctgcctt tcccgcccag cgtgaacacg
     5401 tactggcgac accccaacaa aggggcgttt gcaggtaaga gcctgataag cgcggcgggg
     5461 cgaaaatttc agagcgcggc gtgcgcagca atagttgaac agttacgtcg tctgccgaaa
     5521 ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc ctccggataa ccggatccgc
     5581 gatctggaca actataacaa ggcgctgttt gacgccttga cccacgcggg ggtgtgggaa
     5641 gacgacagtc aggtgaaaag aatgctggtg gagtggggac cggttatccc gaaagggaag
     5701 gtcgagatca ctatcagtaa gtatgagaaa ccggcgggtg cagccgcctg attaagagga
     5761 gaaacgaagt atgaataatc tgatggtcat tgatggtatt gaagttcgtc gtgatgctta
     5821 tgggcgttac agcctgaacg atctgcatcg cgcagcagta gcatctggtg caaatgccag
     5881 aaccaaggag ccaggaaagt ttctttccag ccaacaaact gttgagcttg ttcatgaatt
     5941 gaccaacacc cagaatttgg gtgttgaccc ggtgagtgtg attcatgggg gaaatgaacg
     6001 gggaacgtat gtctgcaagg aactggtgta tgcctatgca atgtggatca gcccgtcatt
     6061 ccatctgaag gtgatccgta ctttcgatat ggtaaccagc gcaccggaaa aattatccgg
     6121 gcaggctgct gacaagatgc aggctggtgt gattctgctg gactttatgc gtcgggaatt
     6181 aaacctgtct aactcatctg tgcttggggc ttgtcagaaa ctccaggagg ctgttggctt
     6241 accgaatctg gctccgcgct atgcaattga tgctcctgcc gatgcacccg atggctcaag
     6301 tcgccctacg ctgtcactga gtgcactgct gaagcagtat ggtatccgcc tgacggctaa
     6361 tcaggcatat caccagatgg tgaagctggg gatcgtcgag cagcgcgaac gatacagccg
     6421 taccgcgatt aacaacatca aaaaattctg gtcgctgaca gcgaaagg
//
LOCUS       DADLSE010000190         6324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLSE010000190 DADLSE010000000
VERSION     DADLSE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.191"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..152
                     /locus_tag="JN692_003743"
                     /pseudo
     CDS             1..152
                     /locus_tag="JN692_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            155..307
                     /locus_tag="JN692_003744"
                     /pseudo
     CDS             155..307
                     /locus_tag="JN692_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1688..2635
                     /gene="icsP"
                     /locus_tag="JN692_003745"
     CDS             1688..2635
                     /gene="icsP"
                     /locus_tag="JN692_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7265919.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQAETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3196..3568)
                     /locus_tag="JN692_003746"
                     /pseudo
     CDS             complement(3196..3568)
                     /locus_tag="JN692_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3571..3756
                     /locus_tag="JN692_003747"
                     /pseudo
     CDS             3571..3756
                     /locus_tag="JN692_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3799..3918
                     /locus_tag="JN692_003748"
                     /pseudo
     CDS             3799..3918
                     /locus_tag="JN692_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4168..5034
                     /gene="ospB"
                     /locus_tag="JN692_003749"
     CDS             4168..5034
                     /gene="ospB"
                     /locus_tag="JN692_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7265920.1"
                     /translation="MNLDGVRPYCRIGNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5363..6103
                     /locus_tag="JN692_003750"
     CDS             5363..6103
                     /locus_tag="JN692_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7265921.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT         1991 a         1184 c         1277 g         1872 t
ORIGIN      
        1 gtggctgagc tggaatctga agttatgcaa ctgcgtaagg ctttaaatga agcacgactt
       61 gagcgagata tattaaaaaa agcaacagcg tattttgccc aggagtcgct gaaaaatacg
      121 cgttagtcga acaatggcga caacatgttc ctaagtggaa tgtctccacg gagaacactt
      181 tatctatcgg gaaatagtgc gggcaacagt gtttaattat atcgagtgcg attacaatcg
      241 ctggcgacgg caccgttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
      301 cgcttaggcc tgtgtccata ttacgtgggt aggctcacag agcctcactt acgactgcgt
      361 ccatattatg tgggtaggat caataaagac gttttatatg tgtctgcttg cagccagaaa
      421 atttataccc tgcaggtatc ataaacatgc ttgataactt aattggggct ccctcttcct
      481 tttatggtgt aattgtttga ttgaatactt ccggggattt cagtatgaaa tgaagtatat
      541 ttaatatact ttacaaaaag tttgtattag tggaataagg acaatagctt atccaaactg
      601 ccaagcctct ttattataag taagatctgg cactggaata ttctcaaaag gactaacatc
      661 atcaccacga ccttcaacca accattttat tgcatttaaa tcccagaata gttcagtagt
      721 ggtcaatttg ggaaacgcac aatattgtac ggttacgtta tcgcaataga gtgaactccc
      781 gccctatacg gcgttttcaa ggattagtcc ttaatcggac aaccacatta aagcataata
      841 aataattaag ctttaataat atactataga tacagttcta ttataaaaaa tactatttta
      901 ggtgatgcat taaattagta gaatatttat tcttttataa aagcaccaat gaattgtcca
      961 agtaaacatg aggtaaaatg tgtagaggat aactgagttt ggtcacttta actgtattag
     1021 tcgcagtctg actctcgact ttaaaaggat gggaattacc tgtactgata gtgagaaagc
     1081 tttccattga aataagtcaa ataaaagttg atggcttcag tgctaaagtg aggtgcccgc
     1141 tagccgtatt cactcgaatc ggaattcgat gtattgcttc tgccatttcc ataatatttc
     1201 acgggaaaaa caaaatctga aaaactatac ttaagtgttg tattgagtca gttgagatac
     1261 aaatacatat ttatcagcac acgttacgtc aaatacagga ggttgtcatg ttggtactga
     1321 aaggcacgtt ggctattgca gccgtgatga caatttattg tcttatcgtt gttcttatgg
     1381 aacgcttttc tgattgattt catattggcg agagaacaag aaggtgagta gaattgtcgc
     1441 ggaatcctga aactatcagc ctcaggtatg aacaccaaaa ggtaggtaga gaaatctatg
     1501 gcccccgtta ctttattcgc acaatgaggt atttttttat ttttacaaaa gttcaataaa
     1561 tattataata aaagatatgt tcttggtaac ataaacaaat atttaattaa aataatgctt
     1621 ggcattgacg tttatcttat aaagtaagaa gatcatggac atttcaacca aaaaagtaga
     1681 gttctcgatg aaattaaaat tccttgtact tgcactttgt gtacctgcga tctttactac
     1741 acatgctacc actaactatc cacttttcat accggacaac atcagtacgg atattagtct
     1801 aggatctctg agtggcaaaa caaaagaacg cgtttatcat cccaaggaag gggggagaaa
     1861 aattagtcaa ctggactgga aatacagtaa tgcgactatt gttagaggtg gcatcgattg
     1921 gaagctaatt ccaaaagtgt ctttcggagt ttccggttgg actactttag gtaaccagaa
     1981 agcaagcatg gttgataaag actggaacaa ttccaatacc cctcaggtat ggaccgacca
     2041 gagctggcat cccaatacgc atctccgtga tgctaacgaa ttcgagctga atcttaaagg
     2101 ttggttatta aataatttgg attatcgact tggactaata gcaggttacc aagagagtcg
     2161 ttacagtttt aatgcaatgg gagggagtta tatttatagt gaaaacgggg ggaacaggaa
     2221 taaaaaaggg gcacatccta gtggtgaaag aacaataggt tacaaacagc tcttcaaaat
     2281 accttatatt ggattaactg ctaattaccg ccatgagaat tttgagtttg gagcagaact
     2341 gaaatatagt ggttgggttc gttcatctga tacagacaaa cactatcagg ctgagacaat
     2401 ttttaaagat gaaataaaaa accaaaatta ctgctctgtt gctgcgaata ttggatacta
     2461 tgtcaccccc agtgcaaaat tttatataga aggctccaga aattacattt ctaataaaaa
     2521 aggtgataca tctctttatg agcaaagtac caatatatct ggcaccatta aaaatagtgc
     2581 aagtattgaa tatattggtt ttctcacttc cgcaggtata aagtatattt tttgaattgg
     2641 ttcaccgagt tgattacgtt ttcatggcta aaaattgccg cccattgtga gtcatattcg
     2701 ccctgacttt ccagaaccat acgaactgcc cgttgacgga cttcggggga aaaacgtgta
     2761 tttttagtca ttctgtttac ctctttctca gggcgtttag tctccaggat ttccggggcg
     2821 gttcaaaaaa ctcttcgttg attactgtgg acttactgtt ggcattaccg accctgagac
     2881 cggagaaata agaaccgctt agatcatcgt ggctgttctc ggggtatcaa gttacacatg
     2941 ggcagagcct acccaaggtc tactattatc gcctgaaagc gctgatggaa cagtgctatc
     3001 agggacatgc tgatgaaaat acctaaataa tcagccagca ctctatcttt ccaaatccac
     3061 agcatagcaa agagagcaaa agagcctgta aattcagaaa ttaaggaaag cctcttggtg
     3121 cacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct ggatcgttcg
     3181 tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct gccgcagata
     3241 ttcccgtggc gagcgataat ccagcgcgct atgcggatgc cattcgttat aatgctcgaa
     3301 tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga tactgatgta
     3361 gtcacacttt attgttttca cgaagctctt tgctattctg ttactctccg gactccgcac
     3421 cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt gtttcattag
     3481 cccggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga agctcgttgc
     3541 cgaagcggtg ttccaccgct cccagcatgt cgccaacggg agagcatcta cggtctgatt
     3601 gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg gctggttaaa
     3661 acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt ccggattgcg
     3721 gaagcggctc tgaataatcc ggatgggctg gttaaaatct gcatattgtt gaaccagatc
     3781 gcagcggtaa gagaagttca tcatacttgg cgcagttacg cgatgtttct gctagaggtg
     3841 atgccgtaaa gaacggcaga acattaccgg aacaagatag cggtctacct gcactggtat
     3901 cagacccggg gcttccccgg atgatctcca cggtacttta ggtactgtgt ctatattatg
     3961 taggtaggat caattaactt tgtagttgaa attctttcat tgaaataatg aataaaaaag
     4021 acaaaccgca ttaaacaatg ccagaatata attgcatttt tgttccgttt ttttaaatca
     4081 tagtatacaa agaagcttgc atactatatg aaaatataac atcctttact atgatgttta
     4141 taaaacaata tatggagtca tgtaggtatg aatttagatg gtgttagacc atactgtaga
     4201 ataggcaata aaaagaatga aagcatatca gatattgcat ttgcacatat aataaaaagg
     4261 gtaaaaaatt catcatgtac tcacccaaaa gcagcattgg tttttttagg agagaaaggt
     4321 ttttgtgata gcaatgatgt tctatctata atgggacaac aaataccaag agtatttaag
     4381 aacaagatgt tatatgatta tgtttttaaa aatgaaaaaa gtaaaaatga ttttctaaaa
     4441 atggctgaat catggctacc acagagtgaa ccaatagtaa taaataatga tgatgacgca
     4501 ttgaatgctg ctgcttattt ttctgtaaaa aaagcgaaaa taaaaacagt aaacgatact
     4561 gattttaaag agtataataa ggtttatatt cttgggcacg gtagtcctgg ttctcatcaa
     4621 ttaggccttg gttcggaact tattgatgta caaacaatca tttcaagaat gaaagactgt
     4681 ggtattctaa atgtgaaaga tatccgtttt acttcatgcg gctccgctga taaagtggct
     4741 cctaaaaatt ttaacaatgc ccctgctgaa agtctttctt gtatccttaa ctctctgcct
     4801 ttttttaagg aaaaagaatc tttgctagag cagataaaaa aacaccttga aaacgatgag
     4861 tcattgagtg atggtctaaa aatatccggc tatcatggat atggagttca ctatggtcaa
     4921 gagctttttc cctactcaca ttatcgttca acttcaattc ctgctgatcc ggagcataca
     4981 gtaaaaagaa gctctcagaa aaagactttt attattaata aagaactgga ttagtataaa
     5041 atttttaacc tatagttaat tgtttgcatt taagatgaag ttattttacc tttagtagaa
     5101 aagtgcttcc ctatgaggga aaatcacaaa tcatcataat acagagacaa aacgatacga
     5161 acaaatagat aaaaaacatc attcttttac cacattattt ttcgtgaata tgaaaaataa
     5221 tgttattact ttaatataag actatttttt gtttttccat cactctgttc aaatttttcc
     5281 gcatgacttg tattttttgt aacacagctc gttttttaca gctgaccaaa atcatcaatt
     5341 aattatgcta aggaaataaa ttatgaaaac caaaaacttt cttctttttt gtattgctac
     5401 aaatatgatt tttatcccct cagcaaatgc tctgaaggca gaaggttttc tcactcaaca
     5461 aacttcacca gacagtttgt caatacttcc gccgcctccg gcagaggatt cagtagtatt
     5521 tcaggctgac aaagctcatt atgaattcgg ccgctcgctc cgggatgcta atcgtgtacg
     5581 tctcgctagc gaagatgcat actacgagaa ttttggtctt gcattttcag atgcttatgg
     5641 catggatatt tcaagggaaa ataccccaat cttatatcag ttgttaacac aagtactaca
     5701 ggatagccat gattacgccg tgcgtaacgc caaagaatat tataaaagag ttcgtccatt
     5761 cgttatttat aaagacgcaa cctgtacacc tgataaagat gagaaaatgg ctatcactgg
     5821 ctcttatccc tctggtcatg catcctttgg ttgggcagta gcactgatac ttgcggagat
     5881 taatcctcaa cgtaaagcgg aaatacttcg acgtggatat gagtttggag aaagtcgggt
     5941 catctgcggt gcgcattggc aaagcgatgt agaggctggg cgtttaatgg gagcatcggt
     6001 tgttgcagta cttcataata cacctgaatt taccaaaagc cttagcgaag ccaaaaaaga
     6061 gtttgaagaa ttaaatactc ctaccaatga actgacccca taaagctgga cagcctgtat
     6121 caggctatgg agggcccata gacaaatcta ccctatatga gcataggagg agtctatggg
     6181 cacaccacgt tttacccttg aatttaaggg attactggaa aggctgggac atatccctcc
     6241 ggcagaagca gaaaaagctt attatgctgc catcggaaac gatgatctgg caacctgagt
     6301 tcacagataa aacattctct agga
//
LOCUS       DADLSE010000191         6251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLSE010000191 DADLSE010000000
VERSION     DADLSE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.192"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(310..1014)
                     /locus_tag="JN692_003751"
     CDS             complement(310..1014)
                     /locus_tag="JN692_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7265922.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN692_003752"
     CDS             complement(1151..1603)
                     /gene="moaE"
                     /locus_tag="JN692_003752"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7265923.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN692_003753"
     CDS             complement(1605..1850)
                     /gene="moaD"
                     /locus_tag="JN692_003753"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7265924.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN692_003754"
     CDS             complement(1843..2328)
                     /gene="moaC"
                     /locus_tag="JN692_003754"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7265925.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN692_003755"
     CDS             complement(2331..2843)
                     /gene="moaB"
                     /locus_tag="JN692_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7265926.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREFEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN692_003756"
     CDS             complement(2865..3854)
                     /gene="moaA"
                     /locus_tag="JN692_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7265927.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDSIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3842..3986)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4251..5159
                     /locus_tag="JN692_003757"
     CDS             4251..5159
                     /locus_tag="JN692_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAY7265928.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASTMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5351..>6251)
                     /locus_tag="JN692_003758"
     CDS             complement(5351..>6251)
                     /locus_tag="JN692_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265929.1"
                     /translation="RPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGL
                     LDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYL
                     HSDIDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERS
                     LIQTIGRAARNVNGKAILYGDKITPSMAKAIGETERRREKQQKYNEEHGITPQGLNKK
                     VVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELEGLMMQHAQNL
                     EFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1505 a         1653 c         1560 g         1533 t
ORIGIN      
        1 tgcggttgcc accaaaccta aagcggtaat gagccatata ccgtttaata tcctggcctc
       61 gtgctgaaaa tctaatttat tgctgttttg cgagtatgat tccattcttt tagttgtcct
      121 tagaaaaaat attaatattc accatcaata aatttaaaac cacgcataca aaatactttt
      181 gtatttatgg atacggaaaa cgattccgct cgctcataag gcatatttta tacctggtac
      241 ttgttaagtg tcgtttttag taagaaaaaa atcccgacac atggccggga tagtgatggt
      301 gaaaaagaat taacggcggt tgccgaagat ccgtaacaac atcaggaaca ggttgatgaa
      361 gtccagatac aaggttaacg cgccaagaat ggaatatttg cgcaggttcg acgtgtcgcg
      421 ggtatcaatc tgctcaccca tatttttcaa tttctgagtg tcatacgccg tcaatccgac
      481 aaagacaatc acgccgatgt aggtaactgc ccacatcaat gcttcgcttt tcagccagaa
      541 gttgaccagc gatgccagca caatgccgat taacgccata aacagcatat tgccaaagcc
      601 acttaaatcg cgcttcgtgg tgtaaccgta caggctcatt gcgccgaaca tcccggcagt
      661 aacgacgaaa gtactggcga tagaagcagc ggtatagaca atgaatatac tggaaagcgt
      721 aagacccgtc agcgccgaat aaagcataaa gagcatcgtc gttacacctg cgctcagctt
      781 ttgaatcatc gctgataaca caataaccaa tgctaattgc gcgatgatca gaccgattaa
      841 aaagacacgg ttagtgaaca acagctccat cacggccgcg gaattagccg cataccaggc
      901 aacaaatgcg gtcagcaaca agccaacggt catccagcca tagacttgcg ccatataagt
      961 ttgcaagcca gcccggggtt gtacgattga atcagaacgt gggaatctgt ccatgacgat
     1021 ctcctgaaga tataaggaat atcttaagga tactgcaaaa tgatgaggct gtgcatcgac
     1081 gcagcgtaaa cgcatgtact gagcggtgaa attgccggac gcagcggtgc cttatccggc
     1141 taacaaaaaa ctaccagcgt tttgccgcct gcagatcgct ctcccgggct tcaacccagc
     1201 ggtcgccttc cggcgtggct tcgcgcttcc agaacggtgc gcgggttttg agataatcca
     1261 taataaactg cccggcttca aacgcactgc tgcgatgcgc actggtgaca ccgacaaaaa
     1321 cgatttcatc gcccggccat aattccccga tgcggtgaat caccgtgacg cgccccagcg
     1381 gccagcggtt acgcgcttca tcaacaattt ctgccagtgc tttttcagtc atccccggat
     1441 agtgttcgag ggttaatgct ttgacgctgt cgcccaggtt atggttgcgc acctttccgg
     1501 tgaaggtgac taccgcaccg tcttcgtcac gctccgccag ccacgggtac tcttctccta
     1561 cgctgaacgg ctgcgggccg acaacaattt tggtttctgc catcttaacc tccggttacc
     1621 ggcgggaaga aagctacttc gtcgccgtca gtcagcggat ggtcaaaact caccagcgtc
     1681 tggttgacgg cagccagcaa tttgccatct tccagcgcca gcgcccagcg atcgctctgc
     1741 gcagccaggt gctggcgtaa cgcttcaacg gttgggtaat ccgcagccac ttcggttgca
     1801 tctgttccca ccaactcgcg cacctgggcg aaaaaaagaa ctttaatcat ccgcttccac
     1861 cttaaagtca cccgacttgc cgccgctctt cgccagcaaa cgtaccggac caatcaccat
     1921 atctttttgc accgctttgc acatgtcata aatggtcagc gccgccacgg aggccgcggt
     1981 taacgcttcc atttcgacac cggttttccc ggtcaggcgg cataaggttt ctatacgcac
     2041 ccgattgtgc tccggctcgg cctgtaaatt gacttcaact ttgctgagca tcagcgggtg
     2101 acacagcggg atcagatccc aagtgcgttt tgccgcctga ataccggcaa tacgcgcagt
     2161 ggcaaatacg tcgcctttgt ggtggcgacc atcaataatc atcgccagcg tctcgctgcg
     2221 catggtgaca aaggcttccg cccgcgcttc acgcacggtt tccgctttgg cggagacatc
     2281 caccatgtgc gcttcgccag cggcgttgat atgggtcagt tgcgacatac ttatttcttc
     2341 aaatgtggat ggaaattaca cggacgcgta cgggcatcca gctgcggcgc gatgatattt
     2401 tcccatgcgg tacggcacgc tttggtcgaa cccggcatgg cgaaaatcag cgttttgtta
     2461 gcgacgcccg ctaccgcacg agattgcaac gtggaagtgc caatctcttc aaacgacaac
     2521 atacggaaca cttcaccaaa accttcaaac tcacggtcga acaacggcag cagcgcttcg
     2581 ggagcctgat caccttccgt caggccagta ccacccgtaa tcaataccac ttgtacatcg
     2641 tcgctggcga tccacgcaga tacctgagcg cgaatagcgt agcggttttc tttcacaatg
     2701 gctttatcga caacgtgatg gcccgcttct tgcgccgaat cgcgcagata gtgaccggag
     2761 gtatcgtctt cttcaccgcg acgattagaa accgtaagaa tagcaatacg ggtcgggata
     2821 aattcagtgc ttacctgact catctgatct ctccttttga cgttttagcc gccaatgtac
     2881 gataagtttt gcgtaatacc ggtgttgttt tgatgcagga aatgggtctg tttcttctcc
     2941 cgcagcgccg ctgaaatacg cgcttccagc gcctgttgct gggtatcgtc ttccagcaga
     3001 tcgcgcaggt taacgccgcc ttcaccaaac aggcagagat ggagtttacc aatggaggaa
     3061 acgcgcaggc ggttgcaagt ggcgcagaag tctttttcat acggcatgat aaggccaatc
     3121 tctccggcgt aatctggatg gcaaaagact tgcgcgggac cgtcgctgcg ttgacgtaat
     3181 tggtggatcc agccgcgacg cagtagctcg tcacgcagaa cctgaccaga gatgtgatgc
     3241 ttacggaaga gctcgctgcc ctcgcccgtt tccatcagtt cgatgaaacg cagctggata
     3301 gggcgatgct ggatccagtt cagaaaggtg tcgagctggt gatgattaac atcacgcatc
     3361 agcacggtat tgactttgac cttctcaaaa ccggcctcaa atgcagcatc aatccctgcc
     3421 atgacctggt tgaatttatc ctgcccggta atagcgtgaa actggcgggc gtccaaactg
     3481 tcgacactga cgttaatgcc agtaagtccc gcatcgcgcc agttcgccac atcgcgttcc
     3541 agacggtaac cattggtggt gaccgcaatc tggcggatag agtcgttttc ccgcacagcg
     3601 gcgatgatat cggtaaagtc gcggcgtaaa gacggctctc ctcctgtcag gcgcactttt
     3661 tcggtgccca gactggcgaa ggcgcgcgta acccggcgaa tttcatcgac ggtaagaaag
     3721 cctttattgg tgacgccgct cggtttgtag ccatccggca ggcagtaggt gcaacgaaag
     3781 ttacacacat cggtaatcga caggcgcaag tagtaaaact tacgcgcaaa tgcatcagtc
     3841 agttgtgaag ccatgtacac ctttccagat acgggaggcg aagtcatttc ttccttcgcc
     3901 ctggtggcaa tctttacgag cattgtcacg gccaaagcac cgtatcagtt gacccaggtg
     3961 cagaggctag agtgtttagt ggttaagccg atactagcgt gtaaatgaga tttttaccat
     4021 ccacactttc gctatgtaat catgtatata gcgtcatgat cgtgctattt cgctacagaa
     4081 taagggaaaa tggcggtttt actttgatat acatcatcag ggaggcatag cgtgcatcgt
     4141 cctttagaga tagaagaaaa tagtttgtat cctcaatatt ggcaggttaa ttgctgtttc
     4201 cccgcaattt gcgctactgt agcgcggata atctgactcc aggaaactat atgcgcaatc
     4261 gtacgctggc tgatcttgat cgtgtcgttg ctctcggcgg agggcatgga ctgggacgcg
     4321 ttctctcatc actttcgtct ttgggttctc gtttaacggg tatcgtcacc accacggata
     4381 atggtggctc gacggggcgt attcgccgtt cagaaggcgg cattgcttgg ggcgatatgc
     4441 gcaactgcct caaccagctg ataacggaac cgagcgtcgc ctccacgatg tttgaatacc
     4501 gttttggcgg caatggcgaa ctttccggtc ataatctcgg aaacttgatg ttaaaggcgc
     4561 tggatcacct tagcgtgcgg cctctggaag ccatcaattt aattcgtaat ctgctgaaag
     4621 tggatacgca tttgattcca atgtcagagc atcctgttga tctgatggcg attgacgatc
     4681 aggggcatga agtttacggc gaggtcaata tcgaccagtt aactacgccg attcaagagt
     4741 tattgttaac gcctaatgta cccgcaacgc gtgaggcggt tcacgctatc aatgaagcgg
     4801 atctcatcat tattgggcct ggcagttttt ataccagcct gatgccaatt ctgctgctga
     4861 aggaaatcgc ccaggcatta cgccgcacgc cagcgccgat ggtttatatc ggcaatctgg
     4921 ggcgtgagtt gagtttacct gcggctaatt tgaagctgga aagcaagctg gcaattatgg
     4981 agcagtatgt tggtaaaaag gtcattgatg cggtcatcgt cgggccaaaa gtggatgtct
     5041 cggcggtgaa agagcggatt gtgatccagg aggtactgga ggccagcgat attccgtatc
     5101 gtcatgaccg ccagttgtta cataacgcgc tggaaaaggc gttacaggct ttaggttaac
     5161 ggaaatccga aggaaaattc cggcttccta ttgaagacaa agtgcgcgtt gtttatgccg
     5221 gatgcggcgt gaacgcctta tccggcctac aaaccgcgca aattcaatat attgcggaga
     5281 aaatgtaggc ctgataagcg tagcgcatca ggctgttttc cgtttgtcat cagtcttctt
     5341 cgctatcctg ttacgatgcg gcaataaaca actcacgcaa ctgatgcaac tggtcacgaa
     5401 tttgtgccgc ttcttcgaac tccagattct gcgcgtgttg catcatcagc ccttccagtt
     5461 catggatttt ctgctgcaac gctttcggcg acatatccat cggcacatta tccggctcaa
     5521 caatcgggcg cgattttcct ctgcccttcg ctttggtttt ggcaatgttc tgccccagcg
     5581 ccaggatatc gaccactttc ttgttcaagc cttgcggcgt aatgccgtgt tcctcgttgt
     5641 acttctgctg tttctcacgg cgacgttcgg tttcgccaat cgctttcgcc attgatggag
     5701 tgatcttatc gccgtagaga atcgctttac cgttaacgtt acgtgccgca cgaccaatgg
     5761 tctggatcaa cgaacgttcg gaacgcagga agccttcttt gtcagcgtcg aggatcgcca
     5821 ccagcgacac ttccggcata tccagacctt cacgcagtaa gttgatccct accaatacgt
     5881 cgaactcacc cagacgcaag tcgcggataa tctccatacg ctcgacggtg tcgatatctg
     5941 agtgaagata acgcacgcgc tcaccgtgtt cttcgagata ttcggtaaga tcttccgcca
     6001 ttcgcttggt cagtgtggtg accagcacgc gttcgttaat tgccgctcgc tgacgaatct
     6061 ccgaaagaag atcatcaacc tgtgtcgcca ccggccgcac ttcgataatc gggtcaagca
     6121 atccggttgg acgcaccacc tgatccacca catcgccgcc ggatttttcc agctcgtaat
     6181 tacccggcgt cgccgaaaca tagatggttt gcggcgctaa tgcttcgaac tcttcaaact
     6241 taagcggacg g
//
LOCUS       DADLSE010000192         6202 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLSE010000192 DADLSE010000000
VERSION     DADLSE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.193"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            186..513
                     /locus_tag="JN692_003759"
                     /pseudo
     CDS             186..513
                     /locus_tag="JN692_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(543..3455)
                     /locus_tag="JN692_003760"
     CDS             complement(543..3455)
                     /locus_tag="JN692_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265930.1"
                     /translation="MFSKAFLRKISMFFARRKQAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTRLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3458..3574
                     /locus_tag="JN692_003761"
     CDS             3458..3574
                     /locus_tag="JN692_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7265931.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3938..4780
                     /locus_tag="JN692_003762"
     CDS             3938..4780
                     /locus_tag="JN692_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7265932.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAEGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4783..5871
                     /gene="capU"
                     /locus_tag="JN692_003763"
     CDS             4783..5871
                     /gene="capU"
                     /locus_tag="JN692_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7265933.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5876..>6202
                     /gene="virK"
                     /locus_tag="JN692_003764"
     CDS             5876..>6202
                     /gene="virK"
                     /locus_tag="JN692_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7265934.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEF"
BASE COUNT         1639 a         1447 c         1484 g         1632 t
ORIGIN      
        1 ggccgccggg gacgtttttt taccaccggg agtttgggca taacggtgct ttcagtaagt
       61 acgacaggta ctgtggaggc acttccgtaa agaagggcat caagctcctg ctccgacata
      121 cctgcaggca aaggccatga aagccctgat ttccggaagc gagcgaacag tccacaaact
      181 gttgaacgtg gtgtcagcgg cggacaggga taagccctcg tcagcagttc cgtcagcact
      241 gcgacagcgt tctttacccg gtcaaaacag cgttgcggtg caccacgcct gacggatgaa
      301 ctgcgtgctc agggttacca ctttaacgta aaaaccgtgg cggcaagcct gcgccgtcag
      361 gggctgaggg caaaggcctc ccggaagttc agcacggtca gttaccgcgc acatggcctg
      421 cccgtgtcag aaaatctgct ggagcaggat ttttacgcca gtggtccgaa ccagaagtgg
      481 gcgggagaca tcacgtactt acgtacagat gaagttcggt tacatcctgt cagcacggaa
      541 cctcacgctt tttgagcggc acgacggatc ccccccaccc agtatccgac agacttttca
      601 ctgcgttcgt aaaaaccctt cttccctccc agcatatcca ctaattccgg tgacatatcc
      661 tgcagataag tctgcaaatc atgccccaga tcttccggtg actgcaactc ataaaccgcg
      721 cgccacacat caccctgcct tattttaagc cccgggttaa gaatatactg tccgtggttc
      781 agcctcagaa aaagcttttt tccataagga tttttgctgt taacttcatg ttgagagagc
      841 tggctgctga tataactcct tcgtttgcgg cgttccggca gcaacgcttc aggaagatga
      901 accacacact ctgccagtcg tgccgcactg aaggcttccg aaaaaaacag agcacttccc
      961 agatgcgtgt ggaacagcgc ctgcatcagg atcaccagaa acattgccat ctgtcggttg
     1021 tccagtttga tgaggcgtcc ttccacctgc agtgacagac tttcaggttt cagccgggta
     1081 tacaactgcg cacttttctg ttgcgcataa cggggcgtac tgaccgccgc tgacagaact
     1141 tgatgataag ccgccagccc gtcattaccg gtcagcaagg gatctgcacc acgctccagc
     1201 agtaactgaa ccagtgcaat attacctgcc cgggctgctg acatcagcgg agtaagatta
     1261 aacggcgaac ggtgatcaac accataacgt tcaatatccc gcaacacggc aaccgggtta
     1321 ttcattgagt atggacggaa atacttcagc tccagtaact gcagagtctt cgttctgggc
     1381 tgccttgtgg gcttaaaatc ttcccggtac agtgcattat acagcgggaa cagggaataa
     1441 atcagactgt actccagcgc ctgcagttgt atggttttat cggctgtacc tgcgaggata
     1501 tgctgtttcc actgtcgtgc atcctctgcg gtgataatct gccatggcat ctcagatgtc
     1561 tgcaggatcc ggctacggat ggtgtccgcc tgctcctgct tcccctgttt ttcaagcaga
     1621 tgtgcggttt tctgccactc atcacggctg gaaatctctt cctgcagatt cagaggctct
     1681 tcctgatgcg tgagcgcaag cagactccac agaggatgct ccacctgctg ttcaacaaga
     1741 tacacattgt gtgtagcacg ggtgagcgca acatacagcg cgttggtaaa aaatttatag
     1801 atttccgctg aacgatcctg cctgtcccgc ggacgggaat aacgtgcctc gccctcaaga
     1861 tcagccggtg taagaccctc gcaaatatca tcaaatgcct gccgggcagc gcttactata
     1921 ttgtaaagga tcaccgtttc gtattccagc cccttaacct cctgaaccga aaagaccagc
     1981 ggcgtactga accagcatcg cgcctgtgct ttctgttccg gatgcatcac aatgaccgct
     2041 acacgattcg aaagtgaggt tttggcattc agttcctgct ttgtctcttc cctgtcgtcc
     2101 agcaaccgga tggtcccttc tgcctgaccg cagcttctga caaaatggtg actttcccga
     2161 tcaatagcac taaagcggac ctgtttgagt ctcagcaacc ggttagccag tgcagtgacg
     2221 tggtgcccgt tacggtaatt cgcctgcagg atattaacgg ttgtgtcgtt tccctgttgc
     2281 tgccggaaaa agagactttt gagcgaactc catgagaaaa agctcggatg aactatctga
     2341 ttggcatcac cacagagtaa aaactgaccg ggatgacgta gcgttttcag taccagttgc
     2401 agttgaacag gcgtcatatc ctgaacttca tcaacaaaaa tgacatcata acggggagtt
     2461 gccaacgaaa gatactgatg actgagcaga ttactgtcat actgatgcga ctgtttcagc
     2521 catgcgatat aacgttcaaa caggacatag accgtcgggc ggtcctccat gccatagagc
     2581 gactgccgta tccccagtga cagatacgcc tcccgggaca gcggcccatt cccggaggca
     2641 accgcaccga tcacgcccct gaactcctca tacagggtat gggcagcccc cagtgcggcg
     2701 atagccctgt tgcggggcag ccagtcgctg aacgcactga aagtcacctc cctgccttcc
     2761 ggaatgcgca gagtttccag aaattccgtc agcgaaagaa agtcaatatt ctgtacctca
     2821 ctcccttccc ctgaagcgtc atagagagta cgcgcttttt ccaccagaaa tgaggaaagg
     2881 ctgagataca gtatatcacc agcagcctgc ttcatttttt ccagtaccag cgccgtctta
     2941 ccacttcccg ccgagccaac aatcaccagc ggtagcgggt gttcgtacag tgcctgctga
     3001 tcatcatcaa aggacagcat tttatccagg cggtggaaac gcccatgaga cggattgata
     3061 tacgtcagcg cttcagtcgc gatatcaaca ggatccggca ctggttgttg ttgtaagcgc
     3121 ccttcatcaa tcaccacgtt tctgcgaaga aaacgtgaag tgttataggc atgattgcga
     3181 atatattcca gaacaaggca gatagtttca ccgtggtagc gatacagcga aaacagtaat
     3241 cggtcccgga tactgagccg ggcgcggtaa agattggtgt caattttacg aacatcagcc
     3301 tgaacaaagt tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca
     3361 ggattcagag tatgataaag acagattttc atggctgcct gtttccgtcg tgcaaaaaac
     3421 atgctgattt tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat
     3481 cagatggaac aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg
     3541 ccgggagggc gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat
     3601 agaagttcgt gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt
     3661 cctttttttc attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt
     3721 agccaaattc agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa
     3781 tatatcattg tttatgaatt gttgatgttt aatttagtaa attatatcaa atatgccatg
     3841 ggtttgctat gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt
     3901 ccttttgtct ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt
     3961 gttcaaagcc aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact
     4021 ggttacactt tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaacaactg
     4081 gaaaacactg tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa
     4141 aagcgttatg ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt
     4201 attaaaagag tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg
     4261 tccggttcgt cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc
     4321 gacagggaat gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc
     4381 tggtctggtc gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag
     4441 ttcacgagaa gaacagtgta aacatcttcg gcaggacttg ttgagcggga gagaatattt
     4501 gaaagaaatg acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa
     4561 ggattatatt caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag
     4621 aatgaatgct cctgctgaag gaactacacg gataggaaga ataagcacca aagaacggga
     4681 aagttgcgca tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt
     4741 gctggccttt cataaggggc caagattacc ggatgattaa taatgaatat actatttacg
     4801 gaatcatcac cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg
     4861 aagaaaatgg ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa
     4921 gccagtaaat tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct
     4981 actgcatgga gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac
     5041 agtgggcatg atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc
     5101 agaattatca ggcaaaagac atatttgaca cgaaaaacaa aagttttttc aataaatcat
     5161 ttttgcgatg aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga
     5221 tgtcgaaccc gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat
     5281 tcccgaaaca gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc
     5341 gttattgctc aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta
     5401 ctgttccatt taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg
     5461 cctgaactgc gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt
     5521 tttattgctg acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt
     5581 ctgccttcag aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg
     5641 cctgtactgg ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg
     5701 acattgttac cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat
     5761 gaccctgggc gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt
     5821 gatattaata aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt
     5881 ttctgtaagt aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc
     5941 actcccgggg aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat
     6001 aaatccggtt gccagtattc gatattatta cgaactgcgt tccttgcagt gcattgagga
     6061 tattctggca atacaaccca cgttgccagc aagaattcat cgaccttatt tgcataaagg
     6121 ggggcgagcc tggtcccggg ggcaatatat tcttgagcat tatcgctttg tgcaaaattt
     6181 gccagaaaaa tattctgaat tt
//
LOCUS       DADLSE010000193         6072 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLSE010000193 DADLSE010000000
VERSION     DADLSE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.194"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..569
                     /gene="yneK"
                     /locus_tag="JN692_003765"
     CDS             <1..569
                     /gene="yneK"
                     /locus_tag="JN692_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7265935.1"
                     /translation="HHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            719..1909
                     /gene="ydeA"
                     /locus_tag="JN692_003766"
     CDS             719..1909
                     /gene="ydeA"
                     /locus_tag="JN692_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7265936.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQAQ"
     gene            complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN692_003767"
     CDS             complement(1934..2599)
                     /gene="marC"
                     /locus_tag="JN692_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7265937.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2811..3245
                     /gene="marR"
                     /locus_tag="JN692_003768"
     CDS             2811..3245
                     /gene="marR"
                     /locus_tag="JN692_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7265938.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3265..3648
                     /gene="marA"
                     /locus_tag="JN692_003769"
     CDS             3265..3648
                     /gene="marA"
                     /locus_tag="JN692_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HAY7265939.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3680..3898
                     /gene="marB"
                     /locus_tag="JN692_003770"
     CDS             3680..3898
                     /gene="marB"
                     /locus_tag="JN692_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7265940.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3929..4756)
                     /locus_tag="JN692_003771"
     CDS             complement(3929..4756)
                     /locus_tag="JN692_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7265941.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATLVPVVGLASAALLLDERLTGLQFLGAVLIMTGLYINVFGLRWRKAVKVGS"
     gene            4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN692_003772"
     CDS             4951..>6072
                     /gene="ydeE"
                     /locus_tag="JN692_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7265942.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNNLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVII"
BASE COUNT         1545 a         1530 c         1396 g         1601 t
ORIGIN      
        1 atcaccatac cgctgcagat tacttacgcc agtttgattt gcttgtcctg aattttatct
       61 ctcgcggtaa ttttgtcata ctcccccgat tatggaataa ctctgaggtt cacagatggt
      121 tcgtcaataa agatcctaac ttgatcaccg ccattctcga cataacggac agcgaattaa
      181 aagaggactt attgcaaagc ctgatggatt cattaggttc taacaaacat gtacaacccg
      241 aagtctgcat ctgcttctta tcccttttag cagaacaaga atctcctcat tttcaggact
      301 tgtttttatt tttcgccaat atgttattgc actatcacca atttatgaat cccaatgaaa
      361 gtgatctgaa tgacgtgttg atgccagcat cattaagtga tgataaaatt atcaaacata
      421 tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac gccgccaaaa gttactcacg
      481 aagacctggt gaaaaacagg cctcgctccc ctgtcagacc acctataccc gcaaccgcca
      541 aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt accgactctc tgacgcggaa
      601 cagaatcagc gtcagagaaa cggaaaacgc gatccagatc acaaatgcat tgtattcaca
      661 tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat
      721 gacaacaaac actgtttccc gcaaagtggc gtggctacgg gtcgttacgc tggcagtcgc
      781 cgccttcatc ttcaacacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca
      841 aagttttcac atgcaaaccg ctcaggtcgg catcatgttg accatttacg catgggtagt
      901 agcgctaatg tcattgcctt ttatgttaat gaccagtcag gttgaacggc gcaaattact
      961 gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagctt
     1021 taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat
     1081 tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgggcacagg ccttgagttt
     1141 aattgccacc ggtacagcac tggcgatggt cttaggttta ccgctcgggc gcattgtggg
     1201 gcagtatttc ggctggcgaa tgaccttctt cgcgattggt attggggcgc ttgttaccct
     1261 tttgtgcctg attaagttac ttcccttact gcctagtgag cattccggtt cattgaaaag
     1321 cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt
     1381 tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtgcaaa acattgcggg
     1441 attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg
     1501 cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct gcgttggtaa gtacggcgat
     1561 tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg gcgaacagtg aaatacacct
     1621 cggggtgctg agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt
     1681 taaagtgctg gcgctggcac cagatgctac cgacgtcgcg atggcgctat tctccggcat
     1741 atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat caggtgagtt tgcactggtc
     1801 aatgtcgatg attggttatg tgggcgcggt gcctgctttt gccgcgttaa tatggtcaat
     1861 cattatattt cgccgctggc cagtgacact cgaagaacag gcgcaatagt tgaaaggccc
     1921 attcgggcct tttttaatgg tacgttttaa tgatttccaa gatgccgtta ataataaact
     1981 gcacacccat acataccagc aggaacccca tcaggcggga gatcgcttca atgccaccct
     2041 tgcccaccag ccgcataatt gcgccggagc tgcgtaggct tccccacaaa ataaccgcca
     2101 ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcgaag gttgaactct
     2161 gacgcactgt ggacgccgag ctaataatca tcgctatggt tcccggaccg gcggtacttg
     2221 gcattgccag tggtacaaag gcaatattgg cactgggttc atcttccagc tcttccgact
     2281 tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg aaaaagcatc cgaaaaccga
     2341 taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat
     2401 ccatcaccag ttgcccagcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg
     2461 ccatcaacga ctgacgatta cgttcagcac tgttcatgtt gcctgccagg ccaagaaata
     2521 gcgcgacagt tgttaatggg ttagctaacg gcagcaacac caccagcccc aggccaattg
     2581 ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctgggttat cagcgaaaag
     2641 tataaggggt aaacaaggat aaagtgtcac tctttagcta accttgcatc gcattgacca
     2701 aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt
     2761 gcctgggcaa tattatcccc tgcaactaat tacttgccag ggcaactaat gtgaaaagta
     2821 ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga
     2881 agaaagatcg tctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta
     2941 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt
     3001 tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg
     3061 ttgaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt accaccagcg
     3121 gcgcggcaat atgcgaacaa tgccatcaat tagttggcca ggacctgcat caagaattaa
     3181 caaaaaacct gacggcggac gaagttgcaa cacttgagca tttgcttaag aaagtcctgc
     3241 cgtaaacaaa aaagaggtat gacgatgtcc agacgcaata ctgacgctat taccattcat
     3301 agcattttgg actggatcga ggacaacctg gaatcgccac tgtcactgga gaaagtgtca
     3361 gagcgttcgg gttactccaa atggcacctg caacggatgt ttaaaaaaga aaccggtcat
     3421 tcattaggcc aatacatccg cagccgtaag atgacggaaa tcgcgcaaaa gctgaaggaa
     3481 agtaacgagc cgatactcta tctggcagaa cgatatggct tcgagtcgca acaaactctg
     3541 acccgaacct tcaaaaatta ctttgatgtt ccgccgcata aataccggat gaccaatatg
     3601 cagggcgaat cgcgcttttt acatccatta aatcattaca acagctagtt gataacgtga
     3661 caacgtcact gaggcaatca tgaaaccact ttcatccgca atagcagctg cgcttattct
     3721 cttttccgcg cagggcgttg cggaacaaac cacgcagcca gttgttactt cttgtgccaa
     3781 tgtcgtggtt gttcccccat cgcaggaaca accaccgttt gatttaaatc acatgggtac
     3841 tggcagtgat aagtcggatg cgctcggcgt gccctattat aatcaacacg ctatgtagtt
     3901 tgttctggcc ccgacatctc ggggcttatt aacttcccac ctttaccgct ttacgccacc
     3961 gcaagccaaa tacattgata tacagcccgg tcataatgag caccgcacct aaaaattgca
     4021 gacccgttaa gcgttcatcc aacaatagtg ccgcacttgc cagtccgact acgggcacca
     4081 gtgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca
     4141 gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     4201 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     4261 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca
     4321 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     4381 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa
     4441 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     4501 caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     4561 atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg
     4621 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     4681 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     4741 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     4801 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     4861 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaaataaa
     4921 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ctctaccagc
     4981 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccatttatg
     5041 accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     5101 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     5161 gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt
     5221 cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa ctgcgcctat
     5281 tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     5341 acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat cggtccgccg
     5401 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt
     5461 tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa aatcatcgcc
     5521 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     5581 tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca
     5641 caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt
     5701 ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg acttaaccca
     5761 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg actggtcggt
     5821 tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt atttactgtc
     5881 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     5941 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca
     6001 ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg
     6061 ttggtgatca tt
//
LOCUS       DADLSE010000194         5960 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLSE010000194 DADLSE010000000
VERSION     DADLSE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.195"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN692_003773"
     CDS             <1..149
                     /locus_tag="JN692_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7265943.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN692_003774"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN692_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7265944.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN692_003775"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN692_003775"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7265945.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN692_003776"
     CDS             1974..2087
                     /locus_tag="JN692_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7265946.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN692_003777"
     CDS             2348..2914
                     /locus_tag="JN692_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7265947.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLLRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN692_003778"
     CDS             2918..3493
                     /locus_tag="JN692_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7265948.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..4587)
                     /locus_tag="JN692_003779"
     CDS             complement(3535..4587)
                     /locus_tag="JN692_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7265949.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4812..5732
                     /locus_tag="JN692_003780"
     CDS             4812..5732
                     /locus_tag="JN692_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7265950.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RITRWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT         1528 a         1451 c         1404 g         1577 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gttgcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttcgcg tccctgatgt tctctgctgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
     3061 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
     3181 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
     4141 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     4201 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
     4261 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     4321 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
     4381 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     4441 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
     4501 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     4561 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     4621 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     4681 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
     4741 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     4801 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     4861 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
     4921 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     4981 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     5041 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
     5101 ggttctggcc ggaccgccga atcacccgct ggacggaagt gatcggcatg gaacacattc
     5161 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     5221 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     5281 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     5341 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     5401 acgcaccgga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
     5461 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     5521 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc
     5581 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     5641 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     5701 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
     5761 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
     5821 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
     5881 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     5941 aacgaaacct gatcgtcgcc
//
LOCUS       DADLSE010000195         5894 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLSE010000195 DADLSE010000000
VERSION     DADLSE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.196"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(209..2062)
                     /locus_tag="JN692_003781"
     CDS             complement(209..2062)
                     /locus_tag="JN692_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7265951.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADAGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPALFTAMLKQFRADLTVFNAQCHGGSAADV
                     PWICGDTTYYWKNTYATQYDTVYGGYKNRESEGVYFVPFMTDGNGVNTATNAPAEDPD
                     IPASGYYGAASRTNGNQVSSNRPTHFSSWARRSIIPDRMATAILNAAGRTSAFISGKA
                     PEIKPSPGGDTPSGPPEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSEGVFKITKQS
                     NKTWSLMHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDDVAMTG
                     TGNPFLMSYFTQTTDGRVNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGSATVTPK
                     LNGLAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLVLEINAPASS"
     gene            complement(2551..2627)
                     /locus_tag="JN692_003782"
     tRNA            complement(2551..2627)
                     /locus_tag="JN692_003782"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2591..2593),aa:Arg,seq:tct)
     gene            complement(2641..2717)
                     /locus_tag="JN692_003783"
     tRNA            complement(2641..2717)
                     /locus_tag="JN692_003783"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2681..2683),aa:Arg,seq:tcg)
     gene            complement(2727..2802)
                     /locus_tag="JN692_003784"
     tRNA            complement(2727..2802)
                     /locus_tag="JN692_003784"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2767..2769),aa:Ile,seq:cat)
     gene            complement(2852..3901)
                     /locus_tag="JN692_003785"
     CDS             complement(2852..3901)
                     /locus_tag="JN692_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HAY7265952.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDGRAALGITAKQIADATGKKNMVPHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYMELLSEYKNLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRAIGVELET
                     GRFEQTVREVQDLIV"
     gene            complement(4010..4250)
                     /locus_tag="JN692_003786"
                     /pseudo
     CDS             complement(4010..4250)
                     /locus_tag="JN692_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4466..4846)
                     /locus_tag="JN692_003787"
     CDS             complement(4466..4846)
                     /locus_tag="JN692_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY7265953.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(4864..5853)
                     /locus_tag="JN692_003788"
     CDS             complement(4864..5853)
                     /locus_tag="JN692_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7265954.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
BASE COUNT         1366 a         1718 c         1405 g         1405 t
ORIGIN      
        1 gttcccaccg ctgacgtggt gtatgacaca cctgttgtga ttttttccac ctggtacata
       61 ccccgtctcc cgttatccgg aagctgacaa caataaaaaa gccaccagtt aagtactgat
      121 ggctctgata actcatgcag gcgtctcaga cgacccactg acactaccgg tgagtttaac
      181 gataccttcc atttgactgg ctcacttttt atgatgatgc cggtgcattt atctccagca
      241 ccagactttc tatctcaacg ccatacgctg cattttttgt aacatccgtc agcgtcagcg
      301 cattcagccc cagtgtcaga ctgtctttta tgacctggaa tgccgggcca gccagtccat
      361 tcagtttcgg agtaaccgtg gcactgccgg cggtgaacac cagctccagc gtctgccagt
      421 cgttaccgta atcgccgaac tcccccagct tcgtgtttcc ggctttcctg tgatgcatca
      481 gattcactct gccgtcagtg gtctgagtga agtaagacat caggaacgga ttaccagtac
      541 ccgtcatcgc cacatcatca ggaacgggag cgtccgtata cagataaatc cccagcccga
      601 actgattgtt ggtcagtgcg cctgagaggc ggaacttaca ggtcagtctg ccgccctgtg
      661 tcagcagggt aattgcgtca tccaccggat gcatcaggga ccaggtttta ttgctctgct
      721 tggtgatctt aaatacaccc tctgacaact gaattccgcc attcttaatg ctccagccct
      781 gcgcagcagc atctccggct gtcggcaaca gggagattgt gcgtacggat gcatcttcag
      841 gcggccccga tggcgtgtcg ccgccgggcg agggtttgat ttccggtgcc ttaccactga
      901 tgaaggctga ggtgcgcccg gctgcgttca gaatagcggt tgccatacga tccggaataa
      961 tgctcctgcg cgcccatgaa ctgaaatgtg tcgggcggtt tgatgatacc tggtttccat
     1021 tcgttctcga tgccgcaccg taatatcctg atgccggaat atccggatct tctgccggcg
     1081 cgttagtggc ggtattgacg ccgttaccgt ctgtcatgaa gggcacaaaa taaacgccct
     1141 cactctccct gtttttatac ccgccgtaca cggtgtcgta ctgggtagcg tatgtatttt
     1201 tccagtaata cgtcgtgtca ccacaaatcc acggcacatc tgcagcactg ccaccatgac
     1261 actgcgcgtt aaacacagtg aggtcagcac gaaactgctt cagcatggct gtaaacagcg
     1321 caggttgctg tgcgtaggtg gcggcgctca tgtcaaactc tccctgcatc cagcacaccg
     1381 ccagcaacac atttttcggg ttcttctgta atgcagcttt agtgcgcgca atcaggtcct
     1441 gatataacgg tttacccacc ccccagcgcg ccgaatcctg gctggccccc gcgtccgcac
     1501 tgaatgtccc ctccgcgccc tgggtgaatg ccgaaccacc acgacagcat ggtaccagca
     1561 ggatccccgc gttattcggg atatacggga gcagtttttt ggcaatatgt aagccctggc
     1621 cgacacagcc gtactgccct ttgctcaggt ctgccttcgg atgattcagc gtactcatat
     1681 cctgcacatc atgcagacag tggtcagccg ggatgatgtc gttatacgta caactctctc
     1741 cacccggagt taccgtgctg cggcgcgcca gctgtttaat gcgcggatcc ggagcatcgt
     1801 aagaatccgg taacggaagc ccttcaccgt aggccatgcc gttggactgt ccggcaagca
     1861 caaccacgta gaaccagtcc ggctcagatg aagggccgac ctgtggatct ccttcaatag
     1921 ccaccgcctg catcagtgtg tacggcgtaa tggcaaccgg tccgccatat ggctgccagc
     1981 cctctttcag tttgtgtgtc agcttttccg caaggtctga cggcgacgcc gccctgacaa
     2041 catcatagtg tttaaatgcc atgaatcctc ccggccggga taatattgtg agtaaaatga
     2101 ggagcgggct gaagtccgga agttacagga caatggcaga agagagacaa cagcccgcaa
     2161 tacgaaaaag gccgcgctat tgcgcagagt gattactgtc ggatattatt cgccagctga
     2221 aatattactt cacgttttgt tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt
     2281 ttttgtccat aagaaagccc ctccggagag gggctggaga gtggcgctat gtgccattgc
     2341 atggtgccgg gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat
     2401 atacctactc gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac
     2461 aaactccccg caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaagg
     2521 cccgcaaaag cgagccggga aaaataagtc tggcgcgttg tactggattc gaaccagtga
     2581 ccgattgctt agaaggcaat tgctctgtcc ggctgagcta acaacgcagg gtacagataa
     2641 tggaccgcca tcgaggactc gaaccccgcg caaccagctt cgaaggctgg cgctctatcc
     2701 cgatgagcta atggcggtat gtgatatggt ggcccttgct ggatttgaac cagcgacctg
     2761 gcgattatga gtcgctcgct ctcaccactg agctaaaggg ccgcgcgcag aataataacg
     2821 ttacggaatt aatactgcaa tctcatccgt ttcaaacgat taaatcctga acttccctga
     2881 ctgtctgctc aaaacgtccg gtctccagct caacaccaat cgcacgacgc cccagtgcca
     2941 tcgccgcttt taccgttgaa cccgacccca tgaaaaaatc cgcaaccaga tcaccaggac
     3001 gactacttgc gctgattatc tgctgcagca tttctgccgg tttttcgcac ggatgtttcc
     3061 ctggatagta ctgcaccggt ttatacgtcc acacatcggt gtacggcacc tgcaccgtca
     3121 caccgaaata ccgccgcaaa tttttatatt cactcagcag ttccatatac tgccggttca
     3181 gctcactgta tgtgctgacc agctggtggt gtggcttttc cagttccccg cgctgatgtt
     3241 tctcttctgc cacccgggca aacagcgact gtaatttcag ataatcgctt tcgttcggta
     3301 gctgccactg actggcactg aaccagtgcg gcaccatgtt tttctttcct gtggcatctg
     3361 caatctgttt tgccgttatc cccagggcag cgcgcccatc acgaaagtaa gcaatcagcg
     3421 gggccatcac atgctgtttc agtgccctgc ccttcgcctc atacccggca tctttcggac
     3481 gatacggccc ctgataatgt tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc
     3541 gcaggctttc cttgttgcat ccgttccagc gtccggacgg cttcgcccag ataatatggt
     3601 tcagcacact gaagcgttca cgcatcatga tttcgatatc agatgccagg cgatgaccac
     3661 agaacaggta aagacttccg gcaggtttca gcacccgcca gaactgcgcc agacactggt
     3721 ccagccactt caggtaatca tcgtcgccct tccactggtt atcccagccc tcaggcttca
     3781 ctttaaagta cggcgggtcc gtgactatca ggtcaacaga attttcgggt aacgaccgga
     3841 taaattccag gcagtcggcg ttgattaact cacaactgga tatttttaca gtattagcca
     3901 tagatcaata agcacttctc tgataggctc ataccgcttt tgcgcaaagc agatgggcct
     3961 gaggtttgct tgtgacccca acgcatgagc agatggctgg tgggtgcccc taacacccac
     4021 cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg
     4081 aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg gcagcgttcg
     4141 cgtgaaaggt aagtattctg cgcaatctcc ccgactgtcg ccggttcggt gacgcttaat
     4201 tcattaaaca ccactctggc ggtttctgtc atatcctgct gtttcagcat gtctttttcc
     4261 ctttttcggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
     4321 caggtattca ccgccaccag cacgtttact gtactggacc gatttcagcc ataaaaaaac
     4381 ccgctcgcgg cgggtttaag ctgtgtggcg aagtaaccac tcttaacata ctgacatact
     4441 ttttgcggac cgcactaatc attttttact tttttagcag ccagtcgtcc atctccagtc
     4501 ttacccccag cacagacaaa catccgtcaa taaacccttc ggctatctgc atctcaattc
     4561 gtattgcttt ttcgcttttc tttctcgtcc tggctatctg tctttttgat attcgcaaca
     4621 aataatgagc aatgagaagc gaatactcct caggtttttt ctgcttcaga cgagcaagac
     4681 agttttcaat gataagtccg tcatcatcgc agcaggccgg acgtggttta gtggcagatg
     4741 gtaaaagtcc tttgaatccg gcagcgatcg gagaatagtc caccccggtg ttaccacttg
     4801 cagcccatgc cccccagcgt tcaagaacca tctgaatatc acgcatcaac tttctccaca
     4861 aaatcaggac agcacaccaa tcgccagcgc gcgatcgata aaacgaaata tcagctccag
     4921 ttgggaacca tacttctctt caaatgccac ggtatccgca tgcagttcgt catggtgttt
     4981 tctgcacaaa ggcaacacaa aaaggtcatg cgcttttgta cccattccac cctgaccatg
     5041 accaatcagg tgatgaggat cgtcggctgg cttaccacaa catgcacacg gctgcgtctt
     5101 aacccagcgc gtgtactttt cattaaccca gcggcgacgt tttgggcgta acataaaaga
     5161 ctccggcgac tccggatcca ctttcagcgc cagcaccttt ttcgccttat cctggatgat
     5221 gctggtggca ggaaccgaag gcacaaggtc actttcccgg gtgacagacg gcacaacagg
     5281 cttcggtaat ctcagtgcct tacgggctgc actttccggt aaggcatccg ccaggtcatt
     5341 acgaatcagc caccagcaca gttccggcat tgtcacaacg tgactgtcat caaaaccaag
     5401 atcacggcgc acaacagaca acacccagcg ggcacagtta tccgttgcca ttgcttccag
     5461 ccgttccgtg aactggtcac ggagaagatt gtcacagtgc cagcacagac ggattgcgcc
     5521 tggcgcgtgc cgcattgtgg tcatgttctc gctgtgccag tcggaatgag gccactgaca
     5581 gcccttttca cgaagtaacc agctctcaag gcattccacg ccaccagcac gacggatcac
     5641 cgcctcatgg cggaacacgg cccgaacggc aggatcatcc gccagcggtt gtgatgctgc
     5701 cggaacggca ccgctggcga aagatgaata acgttccggc tcaggctcca gcaggacacg
     5761 cccctgcata aacaggggca tcagctctga acctggcctg aacaatacga tccccatacg
     5821 cggggcaatt tcaggggtca gtagtgctct cacggtcacc tcaatgaacg gtatcgagca
     5881 gctttaacag ctca
//
LOCUS       DADLSE010000196         5848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLSE010000196 DADLSE010000000
VERSION     DADLSE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.197"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..956)
                     /locus_tag="JN692_003789"
     CDS             complement(<1..956)
                     /locus_tag="JN692_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7265955.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGS"
     gene            1203..1829
                     /locus_tag="JN692_003790"
     CDS             1203..1829
                     /locus_tag="JN692_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7265956.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2941)
                     /locus_tag="JN692_003791"
     CDS             complement(1826..2941)
                     /locus_tag="JN692_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7265957.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN692_003792"
     CDS             complement(3052..3435)
                     /gene="bssR"
                     /locus_tag="JN692_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7265958.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSFTLSIQSLQKHAMLEKA"
     gene            3648..4973
                     /gene="rimO"
                     /locus_tag="JN692_003793"
     CDS             3648..4973
                     /gene="rimO"
                     /locus_tag="JN692_003793"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7265959.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5020..>5848)
                     /locus_tag="JN692_003794"
     CDS             complement(5020..>5848)
                     /locus_tag="JN692_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7265960.1"
                     /translation="SLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYL
                     PTGLYVFAYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNH
                     FERVLDGGLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFD
                     VDKFKNINDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEEL
                     LSILKEIVHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1519 c         1390 g         1457 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgctcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atcggccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcgggatc tagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagcgcgc caataaataa tttttgctgc cactggggga
     2041 atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc cagtaaaaaa
     2101 caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta aagcctgaat
     2161 agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc gggatattaa
     2221 tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc cacggattca
     2281 tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc gcaccggatt
     2341 cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc accagtttgc
     2401 cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc agagcaataa
     2461 aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg gtagacagtt
     2521 ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca tcacttaagc
     2581 ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa taacttaacc
     2641 agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg ccgccctgcc
     2701 cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt cctgcttgcc
     2761 agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg ggtaaaaagg
     2821 ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc gttgcaggag
     2881 ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat tgtcgatgca
     2941 taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata acaaattcat
     3001 ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt ttcaggcctt
     3061 ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gtaaagctca tcagacaatt
     3121 ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc cactcatcaa
     3181 acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc actcttcctg
     3241 atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca gtttaaaaaa
     3301 gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct tcctcagttg
     3361 cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat cgattcgctg
     3421 tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt aaatagtatg
     3481 gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt aacaaaattt
     3541 acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc cagaactctg
     3601 tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg agcaaagtaa
     3661 ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt gtcgattcag
     3721 agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc tatgacgatg
     3781 cggacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa gaatcactgg
     3841 aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt tgtctggggg
     3901 caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc gggcctcata
     3961 gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa cacaacccat
     4021 tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat gcctatctga
     4081 aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct atgcgcggcg
     4141 acctggtgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg gtagatgcgg
     4201 gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt gatgttaaac
     4261 atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc ctgtgcgaac
     4321 agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat ccgcatgtgg
     4381 acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac attccgttgc
     4441 agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta gatcgccaac
     4501 tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc tcaaccttta
     4561 ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac ttcctgaaag
     4621 aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt gcagacgcca
     4681 atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac cgtttcatgc
     4741 agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt gaaattctgg
     4801 tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca gatgcaccgg
     4861 aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt gatatcctgc
     4921 gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt taattaattc
     4981 aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc taatctgtgc
     5041 cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca agcgttcgac
     5101 ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa tgcttcccac
     5161 ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc cgccagtata
     5221 aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta ccgggatctg
     5281 tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat gaccgaaggt
     5341 gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc ctttttccgg
     5401 cagcgctttt aaatcttcgt caaaggattt tctgcccatt gctttggtca ggtcgtccag
     5461 gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa agaaaagccc
     5521 gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca caataccacg
     5581 attaatcact ctgcgtacca gatagaggca actggcaccc gatatcactg ccaccaatgc
     5641 ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat acgcaaatac
     5701 gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg tgaagatggc
     5761 gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata gagagaacat
     5821 caggttctcg ctgtccatcg ccagcgat
//
LOCUS       DADLSE010000197         5773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLSE010000197 DADLSE010000000
VERSION     DADLSE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.198"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(539..844)
                     /locus_tag="JN692_003795"
     CDS             complement(539..844)
                     /locus_tag="JN692_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265961.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(898..1149)
                     /locus_tag="JN692_003796"
     CDS             complement(898..1149)
                     /locus_tag="JN692_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7265962.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1228..2499)
                     /locus_tag="JN692_003797"
     CDS             complement(1228..2499)
                     /locus_tag="JN692_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7265963.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2499..2924)
                     /gene="umuD"
                     /locus_tag="JN692_003798"
     CDS             complement(2499..2924)
                     /gene="umuD"
                     /locus_tag="JN692_003798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7265964.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3025..4245
                     /locus_tag="JN692_003799"
                     /pseudo
     CDS             3025..4245
                     /locus_tag="JN692_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4700..5665
                     /locus_tag="JN692_003800"
     CDS             4700..5665
                     /locus_tag="JN692_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7265965.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1370 a         1529 c         1577 g         1297 t
ORIGIN      
        1 gtatcgccgg tcaggttatc tgcctgggac cacggtccca cctgcatcgt cggcacatta
       61 ttagttaggg acaacggttc aatcaatgcc tgtcacgaac aggtattggc ttgtcgccag
      121 caacccgttg agcggagtaa tcacgcagca ggacggctgt tacaggcgat ggtgaaagct
      181 ctgttgggat ctgaacgaag catgttgagt gaaatttgca aaatttcgca gagtatgcgg
      241 cttcatcttt aaatttccgc cagcattttt aaagccagct accgacgctg cgcgttggcc
      301 ggcccttcgt ccggcgggta accagtaccg gccgatctta cgaccctgga tgaacatcat
      361 cccgtcagca gcgtctccac tgtgatgtcg tttttcacac tgtccgatca caaaatagaa
      421 cgggcaggga caacatatgg taggaaaagc agctgccaga gacacaatat attgattacc
      481 cacagtaacg ggggataacc atgtgggtaa catattgaaa gtgcaacacg ttatctgatt
      541 aattgacttt gtaaacgccg ctgaaccatg aaaaaaaacc gggggaatca tcacagcgca
      601 taaacatacc gttttgtttt tgggtgacgg caaggaggcc ccttttatca ccacatacct
      661 tgtcaaaggt ttccatttct atccttatca ggctcttgtc gttattttca ggagagatcc
      721 ggagatgttt tgtcagccag acaggatagg tatcagcgtt acgccagtac tgcaattcaa
      781 tcactgaagc cattccccag acagcgaagt ataagaaaac cacacctgcg attttgctac
      841 ccatattgtt ccttaacatt ggcttaatac attccttgct cctcgcaccg gagcacatca
      901 cagatactga ttgagatcca cttcacgcag cggctggttt tgacgttctg ttatcgtttg
      961 caccaggtct ttttcgtcca gctcatcgag catttgttca tacaaagccg cgggcacaca
     1021 ataaaaaaca gggcggttac ggtcaattat ctcgaccggg tagccgccgc gggcactggc
     1081 ggtggccacc ggatcgtttt taagttcgct gacgctggca gtcacatcac acagaatgat
     1141 atttgtcatg gttcccccct gagacaagtc acctgtatga tcggtaaagt aagctctggc
     1201 ggcaagtccc ttctgtcatt tcaacggtca gaaacgggca accggcagct ccttccagcg
     1261 tgtggtatat gccggtgaca gcatctcccg tttcatctgc cagtccggcg caataccccg
     1321 tccggcaaac cagactttcc cgagtccgga atgattaatg ccgtcgagta ccttcatcag
     1381 cgcatcgctg tgcggccgtg gctgcacgtc atcgaacaga ttcagctgcg cgacgccgtt
     1441 cggactgaaa tcattgagca ttatccccgc ttttgcgtag cggtggccat cgagccatat
     1501 cctgtccaga gacctgaccg ctgccgcgat aatgtcccgg gtgtcacggg taggggtgtt
     1561 cagtttttcc gtggccacgt taccgtaata cggctcatta accgcaaaag gggatgtctt
     1621 gatgaaggtg ctgatatgcc ggcagtactg tcgctcaccg cgcaactttt ccgccgcgcg
     1681 ctcggcatac tggcagacgg cctggcgcat ttctccgtag gtggtgatcc gctgcccgaa
     1741 gctgcgactg cagacaatct gctgtttcgg cggcggcgct tcctccagcg aaatgcaact
     1801 ctcgccgttc agctcacgta ccgtgcgctc cagcactacg ttgaagtttt tccggataaa
     1861 tgtagggttc gtcagcgaca gctgcagggc ggtggtgatc cccataacgt gcagcttttt
     1921 cgctatccgg ttccccacgc cccagatttc ttccaccggc tgcaggctga gtaattttgc
     1981 cgttctgcgt ggattttcgg gcgacagcgc cagcacccca ctgaattgtg gccattcctt
     2041 tgacgcccac tgcgccgatt tggccagtgt ttttgtggcg ccgaagccga caccgatcgt
     2101 cagtccggtt ccactgagaa catgcccacg cagctgcctg ccaaaatcct ccagatccat
     2161 acagtggcta attccccggg catccaggaa acattcgtca attgagtatt gttcgacgcg
     2221 gggcgacagc tcttccagca gggcgaccac acggttactc aggctcgcgt agagctcgta
     2281 gttgctggaa aaaacgtaca gtttttcagg aaactgcgcc tctttgagct ggaaccaggg
     2341 ggttcccatc tttataccca gccgtttggc ttcagcactc cgggcaataa cgcagccatc
     2401 gttgttactg agaacgacga caggtttgcc gcgcaaatcc ggcctgaaaa ccttctcgca
     2461 actggcgtag aaggagttga cgtctgccag ggcgaacatc agtctgccct ccgggtttta
     2521 tgaatgaagg ccgtcaccac gccgaaaatt tgcagagttt ccggatccgg gtacagggaa
     2581 gggaagtccg ggttcatcgc ctgcagggct ggccgcggcg tcagaagcag gcgttttacc
     2641 gtaaattcac cctcaatttc cgcgataaca atatccccgt gccgcggctg ttctgcttta
     2701 tccacgacca tcagatcgcc ggaatagagg ccaatatcag tcattgagtt gccaatcgct
     2761 ctgacaaaat aggtggagtg gcgccggtga atgcagtact cattcagatc cagttcagac
     2821 tcagtatagt ttgctgccgg tgacggaaaa cccgcagggc atcgctctgt gaacaaagga
     2881 atttttacta tgctggcttc tgatactgca ggaatgaact tcatcgtttt ttccctcgct
     2941 taaaactgta tatgcatggg tcttcccttg ttgtggtggc tgaaggcatg ataatggtgt
     3001 atttaatcgc cagaggtcac cgccatggac gaaaagtccc tctacgctca tattctcaac
     3061 ctgtccgatc cgtggcaggt aaagtccctt tctctcgatg aaaatgccgg ttctgttact
     3121 gtcactattg agatcgctga aaacacccgg ctagcctgtc cgacctgcgg taaatcctgt
     3181 tctgttcacg atcaccgtca tcgtaaatgg cgccatcttg atacctgcca gttcaccact
     3241 attgttgaag ccgatgttcc acgaattatg tgtccggagc atggctgcct gacgttgcct
     3301 gttccgtggg ctggccccgg aagccggtat acgttgctat tcgaatcgtt cgttctctca
     3361 tggctgaaaa tcagcaccgt tgatgctgtc aggaagcaac ttaagctcag ttggaatgcg
     3421 gttgacggca ttatgacccg ggcagttaag cgaggtcttg cccggataaa aaagccatta
     3481 tccgcccgtc atatgaatgt ggatgaggtc gcctttaaaa aaggacatcg ttacataacg
     3541 gtgatctccg atcgcgatgg tcgggcgctg gccttaacgg atgatcgcgg cacagagagt
     3601 cttgccggct atcttcgcac gctcactgat gggcagttgc tggctatcaa aacgctctca
     3661 atggacatga acgcgggcta tataagagca gcgcgtatcc acttacccag tgcggttgag
     3721 aaaatcgcct ttgaccgctt ccatgtggcg aagcaactgg gcgaggtagt tgataaaacc
     3781 cgtcagaatg aacatccgca cctccctgtt gaaagccgac accaggcaaa aggaacccgc
     3841 ttcctgtggc agtacagcga taagtggatg accgaatccc ggcaggaaaa gctgatgtgg
     3901 ctgcgtgcac agatgaagct gacgagccag tgctgggcgc tgaaagagct ggcaaaggat
     3961 atctggaaca ggccatggag cgaggaaaga cggagtgact ggcagagatg gttggcgctg
     4021 gcggctaaca gtgacgttcc catgatgaaa aatgccgcga aaacgatagg aaaaaggctg
     4081 taggggatcc tgaatgcgat gcgacacagt gtctcaaacg gaaatgcgga ggcacttaac
     4141 agcaagatca ggctgctgag gataaaagcc aggggatacc gaaaccggga gcgctttaaa
     4201 ctgggggtga tgttccacta cggaaagctg aatatggcgt tctgagcctt cccaccatga
     4261 tcggggaaga cccatatgca tacagtataa ggggcggtga aatttctgca agtgatgggg
     4321 ggatagccgg tcagaacgtc cccgaaaaca aaccgccccc ggtttcagga agggcgaact
     4381 gaccggcaga ggcagtgtaa caccgcatgt cacaaaggca gcactgacgt cgtcagtgct
     4441 ttttttaccc gtcctcccgg acgctgaagg gcagtttcac ccgtgcagct aaaggggcgg
     4501 taagtccggg tgtgcacatc gtcattttct cctgtgatgc ctttctcctg cgtatcagct
     4561 tccggtaatc gtcccccgtc acaacctgga cagcaagggt aaatggcacg ctgcgcgccc
     4621 ttgctgacca gaacgactcc gggcaggccg aaccgtcagg cgatacgaag acgaaattca
     4681 cactgacgga gaaacagcca tgacgatgaa cctgcacact cagacaaccg cccctaaagc
     4741 ttcacaggcg accagcgtat ccccgctgga gcagtcaggc tcctcaaaaa cgaaattttc
     4801 caggggcaaa agcaaagccc gtgctggcgc agtcaaaaca gacctttacc agacagtaac
     4861 ggacagcatc attgcagccc tggaaagcgg cgttaagccg tgggtgtgtc cgtgggttcg
     4921 caacggcgca gcggtgggat taccggccaa cttcagcacc ggcacggcat acagcggaat
     4981 taatatcatg ctgctgtggt gtagtgcggc aaaacagggt tttcaggacg agcgctggct
     5041 gacctacaaa caggcgcagg agctgggcgg ccaggttcgt aaaggtgaac acggcacgac
     5101 ggccatcttt tacaagacgc tggagaaaga ggacgaggac ggggaaatcg aaaaaatccc
     5161 gatgctgaaa gcctttaccg tgtttaacgt ggagcaaatc gacggcctga gcatcgagag
     5221 cgtgccgcag ccggttgccg gtttcgatcc gctgccgcag gcggaagcac tcatgatccg
     5281 gagcggggca aaaatcaccg agcagggcgt aaaggcgttc tatcgtccgg ccaccgatga
     5341 aatcgtcctg ccggagcggt tccgcttcgc ggacgcggcg aacttctacg ccaccggcct
     5401 gcatgagctg gttcactgga cgggtgcagc gtcccgcctc aatcgtgaaa aaggcaataa
     5461 gtttggctct gaggcgtatg cgtttgagga actgatcgcc gagctgggca gcgcgtttct
     5521 gttggcggat ttggggatta cgggagaggt gcagcatgaa agctatatcg cgtcctggct
     5581 gaaagcgctg aagggagaca agcgctacat ctttaaagcg gcggcagcgg cttcaaaagc
     5641 gcaccgctgg ctgatggact gctgacagct ggagccctgc ggtgcagggc ttcagaattt
     5701 ttgaaagaga gagaatcagg gagggaatta tataagtcac tgttttttag aattctgtaa
     5761 cgcggccggc ctc
//
LOCUS       DADLSE010000198         5772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLSE010000198 DADLSE010000000
VERSION     DADLSE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.199"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..391)
                     /locus_tag="JN692_003801"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="JN692_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN692_003802"
                     /gene_synonym="virG"
     CDS             complement(577..3885)
                     /gene="icsA"
                     /locus_tag="JN692_003802"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7265966.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPVAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINTDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSMINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKDRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4414..5616
                     /gene="virA"
                     /locus_tag="JN692_003803"
     CDS             4414..5616
                     /gene="virA"
                     /locus_tag="JN692_003803"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7265967.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1784 a         1310 c          955 g         1723 t
ORIGIN      
        1 aggctgtacg cttccccatg tgatttgcct cgccacttca ccgggctcag gaccccgtag
       61 gatcttcacc gaaaaaaatg catcaccagt tgctttatta tttgttttag gtagacctgt
      121 tactaattta tacctactct gaccaagtct ggataagaaa ggcacacctg cacatttcta
      181 cttggaaatg ttttctttta ccattttgct caaaaatttc aacatccaat attcctcgta
      241 cagaactact caagtcctga atagaaaaag ttccagctgg tacagtagag tcatatatta
      301 ctcgaccttg ttgagataca acaacacgtg cattagtttc tgcaatacca gtaatctgag
      361 gcgcataacc acgaatattt ggcggtagca ttcgttcatc gctttctaat gcaattcctg
      421 tgtaacgcca agcaacagcg cattctggcg catgatctgg taaacacgtt tggcattgat
      481 cgcaggcata ccatcatgtg cacaaaaacc caccgagctt ttacacggtg ggtcccagag
      541 aaatgcagga catcaacacg ccctgcattt ttattatcag aaggtatatt tcacacccaa
      601 aataccttgg gtgtctctgt aactgttatt acccaattgc tgagatacgt ttccccagat
      661 gcttaggtta tctgcaagac gcccccccac accagttttg agttcaatca cattacggtt
      721 gcctatctgg tgatttgaca tgccattcat aattacacca tattgctttg agctccattt
      781 ccaattcact tccaaaaatg gttcgaactt acgaagagtc tccttatcga tacaactctg
      841 actaattaag gatgtacgca atcccaattt tgcttgaata ttattgaccc cagaggatgt
      901 gaccatagtg ccattgtgtt caatatagtc gtttgctttt accccatgcc tagttatctg
      961 gacttgtgga ttgagataca atgcgttgtc aatattatta tgagcagtcc agcgtatggt
     1021 tgggtagata tagccagctt ccaaagctcc tgttattcct ttgctggaat atttctcacc
     1081 agttaaccca tctcctttga ctgtattatt aaaccaaccg tactgcaacc aagcatccat
     1141 atatagacct gttcgttctt tttcatcctg aaaccaagaa ctgtacaaac cggcagtata
     1201 accgctcaca gttcctcgtg aactataact agtatactta ttatgagttt tgctatcctg
     1261 atatccataa gctcccataa tacccacatg caagccatca tgatcagtga agtttgtctt
     1321 caaaatatct ccaccaatct gataaaccat cctgttagct gtggttttta attgtctatc
     1381 agctaaatta tgctcattat gtccaccaac agtacgaatc cacatggtgg tttcgctgag
     1441 ctgacctgta gtaggatcaa caatagtgct accgtgtcgg tcatagagag aaggactaaa
     1501 catcgtgttc attgctatca ggttagcaat ataactgcca gcttcagggc gccatgtgtg
     1561 aatactacct tcacccaaat taagtgaaga tacaagctgt gaactagctc ggcgactact
     1621 catttgagta gactcttgat tgctcatctg ttttgattct tgattatcca tctgactagt
     1681 tagataccac ttattggtat tcagtccaga tacagtgccc tgtttcaggc gatagtcata
     1741 actaccagca acaatacggt ctttctgaat aaaagcattc ttatcagaag aatctgtcga
     1801 aataatttga acaccttcaa gagttttatc tccagagcct ccctcatttg tcactgcaac
     1861 ataggtaata ccagacgcat ggcctgcaat ttccagccgg tcagttttac tgttgtcatt
     1921 gccgagaacc gcgcccaaag ataatattcc gccctttcca tgccaattac ctttctgaac
     1981 ataggtctga ccgacatttg acgaactatt attgagaata acatgaccgt ttttctccag
     2041 agtcatgtta cctgtgacaa tgacggggtc aggcaaaaaa ggggcattaa tattattgat
     2101 tagcacagtc ccactattta ataaagtgtt aacagtttgg ttcatgcctg aaaaattcaa
     2161 ggtagcacct ttattaacaa taacaccagc ggtacgtgtc atagcttcaa ctgtccccat
     2221 ttttagaatg cctgcattga tgttggtgaa accagagtag gtattttttt ccgccaaaat
     2281 gagagttccc tcccctgatt ttgttaggga ttttccgtcc cagctattta tattatgatg
     2341 gctgagatta tctgccagaa tagtactaac agtaagttca gcgttttctt tcagattgaa
     2401 ctctccatga ctgtcgccat ctgtatcatt ccagctcaaa ccgtagatca atttattctg
     2461 gtcactgata tagctttttg tatagtcaat agcaccagta gtgagtaacg gattgatgat
     2521 agatatatta gaaacactga aacctgttat ccctttatct gcacttagca gtttatagtc
     2581 cttctgcgcc cagttattta taatagcttc cacttttaca ttgttgccaa atgacatatt
     2641 tccagaagct tccagactac tgattccagc taaatttaaa gaactgttct caccaaaaga
     2701 aacgtcacct gaaacagtga atttgttacc tgatagtgaa acagttgtac aatcaccagc
     2761 acataaatca ccttcaatag ttggtgtggt cccagtgtaa tcattgttga taattttcag
     2821 aatggatgaa ttgttaacct gtacatcacc agtaatgaca gaaccttcat ggagtattaa
     2881 tgaattgttt ccaccagtga tgtggatagc attacctgct acattttgtg ttccatcatc
     2941 ttctttacct gaaattatat atccgctatt agcaatggtc atattgctac catttatagc
     3001 atccccccaa tgtccaccat taccgcctaa aatagctcca ttgttgatca tagacaggtt
     3061 atctcctgtg atagcattac caccgtagcc atcataacca taaccgtaag cagcacctcc
     3121 gttgcctcca gcgaacgtgc ccttattgat tacagagagg ctgcttcctg tgatggcatc
     3181 acctccatta ccgccatcac cctcaccata attgttacct ccattaccac cggaaatagt
     3241 ccctttgttg attatgggta gattgactcc tgtgacggca tcacctccat tccccccatc
     3301 actatcacta taactatcgc caccatgtcc acctgaaata atttctccat ttattataaa
     3361 cagattgtct cctgtaacag cctcaccacc atcaccgtta tggtcagcac cgctaccgcc
     3421 gctaccacca agaatcatgc cttgattgat tatagacaag tcagatccgg taatagagtc
     3481 accaccatta ccgccacaac tattaccatc attattatca ccagcaccac catgacctgc
     3541 gctaatcata atatcagtgt taatccgtag cttagttcta gatgcatgag aggggttgga
     3601 agaagttata taccacgcat ccattccatc ttcaccagct cccaagggag aaagtccatc
     3661 aacaggtgtt aataattttt cataattgtc ctctgaaaaa tgaagttctt gagtacccga
     3721 aagaggaata gcaaaagcta ctggcccccc gagcaacagg gatgcaccaa caacaaaagg
     3781 agaaaaagac aatttgcatg ttttttcctt taccgtaggt aattctccgg cccccccctg
     3841 tgaacattgg gtcatattac aaaaaaattt gtgaatttga ttcatgcact atattatcag
     3901 taagtggttg ataaacccct aaaaagattc cgttttatcc ggaataaagg gacgatatat
     3961 gcaaaaacat attaaacaaa gcctcaacca aaacatgaga gctgtgttat atatcaatgc
     4021 aacgggagaa atttacctgt gtcttgcttt gaaaaaaaga gggggaaagc tatatgttcg
     4081 ataataaatt aataatttta ctatgatagt ctgcttcaga ccgcaacttt tcagttcttt
     4141 ctgtacgctt gcccaaagtt taaccacttt gtttttctat acacccaatg gttctatctg
     4201 ccccttgatg ttaatcccca aagccaataa catgaatgta ttcatatcac aactttccta
     4261 ccagactctc caatgcaata actgcataat tgtttttagt ggaaatgtat cgttttctta
     4321 aagagaagaa taacattcca ctttattatg ttcccaattt aattatatta atatatattg
     4381 gcattaatag gaaaatacat caggagaaat caaatgcaga catcaaacat aactaaccat
     4441 gaaagaaatg actcatcatg gatgtcaact gtcaaatcaa ctacagaagt aagttggaat
     4501 aaattgagtt tttgtgacgt tttactgaaa ataataactt tcggcatata ttctccacac
     4561 gaaactctag ccgaaaaata tagcgaaaaa aaactaatgg atagtttctc accatcacta
     4621 tcacaagata aaatggacgg agagtttgcc cacgcaaaca tagatggcat ttcaatacgg
     4681 ctatgcctta acaaaggcat atgttcggtt ttttatcttg atggagataa aatacaatcc
     4741 actcaacttt catcaaaaga atataataac ttgttaagtt cactcccccc caaacaattc
     4801 aacttaggaa aagtacatac tattacagct ccggtatcag ggaattttaa aactcacaag
     4861 cctgcaccag aagttattga aacagccatt aattgctgta cctcgataat accaaacgat
     4921 gattacttcc ccgttaaaga cactgatttt aatagtgttt ggcatgatat ttacagagat
     4981 attcgagcat ctgacagcaa tagtacaaaa atatatttca acaatattga aataccacta
     5041 aaattaattg cagatctaat taatgagtta ggaattaatg aattcattga tagcaaaaaa
     5101 gagcttcaaa tgctaagtta caaccaagta aataaaataa tcaattcaaa cttcccccaa
     5161 caagacctat gttttcaaac agaaaaactt ttgtttactt cactctttca agaccctgca
     5221 tttatatctg cactaacatc tgcattctgg caatctcttc acatcacgtc ttcctctgtg
     5281 gaacacatat atgcccaaat tatgtccgaa aacatagaaa acaggcttaa tttcatgcct
     5341 gaacaacgag ttattaacaa ttgtggacat attataaaaa tcaacgcagt tgtccctaaa
     5401 aacgacactg caatctctgc ctctgggggg agggcttacg aagttagctc atcaatatta
     5461 ccatcgcaca taacatgtaa tggtgttggt attaataaaa tagaaacaag ctatctcgtt
     5521 catgctggaa cacttccaag cagtgaaggg cttcgcaatg ctatccctcc agaaagtcgt
     5581 caagtatcat ttgccatcat atctcctgat gtttaagcca atattatatt ggctgccaga
     5641 ctgtaaaaaa acgttcttca gaacagctct ataaactatt aaaccaaagc taaaaggatg
     5701 gggaccaata gaatctcagc ccatcctttt tatatcatta tgaaccgccc cggaaatcct
     5761 ggagactaaa ct
//
LOCUS       DADLSE010000199         5711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLSE010000199 DADLSE010000000
VERSION     DADLSE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.200"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1537)
                     /locus_tag="JN692_003804"
     CDS             complement(<1..1537)
                     /locus_tag="JN692_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7265968.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPMQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHFSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAM"
     gene            complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN692_003805"
     CDS             complement(1744..2028)
                     /gene="ihfB"
                     /locus_tag="JN692_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7265969.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN692_003806"
     CDS             complement(2188..3861)
                     /gene="rpsA"
                     /locus_tag="JN692_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7265970.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN692_003807"
     CDS             complement(3972..4655)
                     /gene="cmk"
                     /locus_tag="JN692_003807"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7265971.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4828..5592)
                     /locus_tag="JN692_003808"
     CDS             complement(4828..5592)
                     /locus_tag="JN692_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7265972.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1447 a         1507 c         1346 g         1411 t
ORIGIN      
        1 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
       61 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
      121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
      181 aacattcagc aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
      241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
      301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
      361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
      421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
      481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
      541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
      601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
      661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
      721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
      781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
      841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
      901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
      961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
     1021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
     1081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
     1141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg ccatacagca
     1201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
     1261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
     1321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
     1381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
     1441 tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac ggaaaaattc
     1501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
     1561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
     1621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
     1681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
     1741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
     1801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
     1861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
     1921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
     1981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
     2041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
     2101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
     2161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
     2221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
     2281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
     2341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
     2401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
     2461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
     2521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
     2581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
     2641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
     2701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
     2761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
     2821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
     2881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
     2941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
     3001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
     3061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
     3121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
     3181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
     3241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
     3301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
     3361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
     3421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
     3481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
     3541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
     3601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
     3661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct cagccgggat
     3721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
     3781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
     3841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
     3901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
     3961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
     4021 acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
     4081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
     4141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
     4201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
     4261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
     4321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
     4381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
     4441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
     4501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
     4561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
     4621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
     4681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
     4741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
     4801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
     4861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
     4921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
     4981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
     5041 aattgcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
     5101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
     5161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
     5221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
     5281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
     5341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
     5401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
     5461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
     5521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
     5581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
     5641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
     5701 ccgacaatgt g
//
LOCUS       DADLSE010000200         5697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLSE010000200 DADLSE010000000
VERSION     DADLSE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.201"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            67..282
                     /gene="xisR"
                     /locus_tag="JN692_003809"
     CDS             67..282
                     /gene="xisR"
                     /locus_tag="JN692_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HAY7265973.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            284..1519
                     /locus_tag="JN692_003810"
     CDS             284..1519
                     /locus_tag="JN692_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7265974.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRHNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            2006..2896
                     /gene="sitA"
                     /locus_tag="JN692_003811"
     CDS             2006..2896
                     /gene="sitA"
                     /locus_tag="JN692_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7265975.1"
                     /translation="MLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDNPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
     gene            2896..3723
                     /gene="sitB"
                     /locus_tag="JN692_003812"
     CDS             2896..3723
                     /gene="sitB"
                     /locus_tag="JN692_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7265976.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            3720..4577
                     /gene="sitC"
                     /locus_tag="JN692_003813"
     CDS             3720..4577
                     /gene="sitC"
                     /locus_tag="JN692_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7265977.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            4574..5431
                     /gene="sitD"
                     /locus_tag="JN692_003814"
     CDS             4574..5431
                     /gene="sitD"
                     /locus_tag="JN692_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7265978.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1517 a         1305 c         1373 g         1502 t
ORIGIN      
        1 cctcattaat aatcaacatc gggcgtcaat ttcagcctaa cattggcgcc tgccagaggt
       61 gatgcgatgg cacaagtaat ctttaatgaa gagtggatgg ttgaatacgg cctgatgctt
      121 cgcactggtc tgggtgccag acaaattgaa gcataccgcc agaactgttg ggtggaaggc
      181 ttccacttca aacgagtatc tcctttaggg aagccagaca gtaagcgagg gattatctgg
      241 tacaactatc cgaagataaa tcagtttatc aaagactcat gatatgtcta aattaccaac
      301 aggtgtcgag attcgaggta aatacattcg catctggttc atgtttcgag gaaaacgatg
      361 tcgggaaaca ttgaaaggct gggagattac taacagtaac attaaaaagg ccgggaattt
      421 aagagcgttg atagttcatg aaatcagttc cggtgaattt gagtatttaa gacgttttcc
      481 ccagtccagc actggggcta aaatggtgac aacgagggtc ataaaaacat tcggggagct
      541 ttgtgatatc tggacaaaaa ttaaagagac agagttaaca acaaacacaa tgaagaaaac
      601 gaaatcacaa ttaaaaacac tcaggataat aatttgtgag agtaccccga tatcgcatat
      661 tcgttatagc gatatcttaa actaccggaa tgaactgctg catggagaaa cgctttacct
      721 agataatcca agatccaaca aaaaaggaag aaccgtgcgc acagttgata actatatcgc
      781 cctgctctgt tcgttgttac gttttgcgta tcagtcggga tttatatcaa ccaaaccatt
      841 tgaaggagta aaaaaattac agcgaaacag aataaagcct gacccgttat ctaaaacaga
      901 attcaatgca ttaatggaaa gtgaaaaagg acagagccaa aacttgtgga aatttgccgt
      961 ttactccggg cttcgtcacg gggaactggc agctctggcg tgggaggatg tggatttcga
     1021 gaagggaatt gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa
     1081 aacaaatgcc gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga
     1141 gcaatacaaa ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta
     1201 cggcagaact gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa
     1261 acagaaacct tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg
     1321 tgctggtatt cgccgccata atccgtacca tacgcgacat acttttgcct gctggctgtt
     1381 gacggcagga gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat
     1441 ggtgtatgaa atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt
     1501 gaatgcgcgg ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag
     1561 aactgaaata atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa
     1621 gtaagggcaa aaatcacaac tatctgactc ataagtattt tatttatttt tcaatatgtt
     1681 aaacattatc agcgacaaca ataagctacg ataacccact ctttttttgc cccttttttg
     1741 ccccttctac agcgttttgc cccatttttg ccatcaacaa ttttcctcat aaagtcattt
     1801 caaagcctaa tagctgctct acttttttcc tcaatatcat gtcgccagca ggaaacaacc
     1861 taatccctgt tttaggcctc aaagctgtta atgataatta ttttcatttt gtataggtta
     1921 tgctataaaa catagccgtt gctatgtttg ttctaatttt gctcactata ggtactaaat
     1981 tatgcactca ataaaaaaag taaccatgct cttggggtgc ctcgccctca cctgctcgat
     2041 cgcatttcag gcaagtgcaa ctgaaaaatt caaggtcatt acaacattta ccatcatcgc
     2101 ggatatggcc aaaaacgtgg ctggagatgc tgcagaagtc tcatccataa ccaagcctgg
     2161 tgcagaaatt catgagtatc agcctacccc tggcgatatt aaacgtgcgc agggggcaca
     2221 actgattctc gccaatggta tgaatctgga attgtggttc caacgctttt accagcatct
     2281 caatggggtt ccagaagtaa ttgtctcttc gggtgtgacg ccagtaggga tcaccgaagg
     2341 accctatgag ggcaaaccta atccccatgc atggatgtcg ccagataatg ctctgattta
     2401 cgtcgataat attcgtgatg cgttgataaa atacgatccg gcaaatgcac aaacctacca
     2461 acgtaatgcc gatacttata aagccaagat tacccaaacc cttgcccccc tgcgtaagca
     2521 gattacggaa ctccctgaga atcagcgatg gatggtcacc agtgaagggg ctttttctta
     2581 tctcgcacgc gatttggggc taaaagagct ttatctgtgg ccgattaatg ccgatcaaca
     2641 aggaacaccg cagcaggtac gtaaggttgt tgatatagtt aagaaaaatc atataccggc
     2701 agtctttagc gagagtacga tttccgataa tccagcgcgt caggttgcgc gtgaaaccgg
     2761 cgcacactac ggcggtgtgc tctatgtcga ttccctgagc acagaaaacg gcccggtacc
     2821 tacgtatatc gaccttctta aggttactac cagtacgctg gtacagggaa ttaaagccgg
     2881 gaaaagggag aaataatgat gcaatctgca ggcattgtcg ttaatgatgt tactgtcacc
     2941 tggcgtaacg ggcacacagc actgcgcgat gcatccttca cagtaccaag tggatctatt
     3001 gccgctctgg ttggggtaaa cggttccgga aaatcgacgt tgtttaaagc gattatggga
     3061 tttgtgcgtc tgaccagcgg aaagatatct gttctgggta ttcccacgcg acaggcgcta
     3121 cagaaaaacc tggttgccta cgtccctcag tcagaagagg ttgactggtc atttcctgta
     3181 ctggtggaag atgtggtgat gatgggccgc tatgggcata tgggtatgtt gcgtatagcc
     3241 aaaaagaggg atcgtcagat tgtcaccgat gcgctggaac gtgtcgatat ggtggatttt
     3301 cgccatcgtc aaatcggcga gctttccggt ggacaaaaga aacgcgtctt tctggcgaga
     3361 gcgattgcac agcagggtga tgtgatcctg ctggatgaac cttttaccgg cgttgatgtg
     3421 aaaaccgaag cgaaaatcat cagcctgttg agggagttac gcgcggaagg caaaacgatg
     3481 cttgtctcaa cgcacaatct cggctcagtc acgacatttt gcgattatac ggtcatggtc
     3541 aaaggcaccg tcctggcaag cgggccgaca gacaccactt ttacagccga gaacctggag
     3601 ctggctttta gcggcgtact gcgccatgtc acacttaacg gctcggaaga aagcattatt
     3661 actgacgatg aacgcccttt tgtggcacat cgaccgtcag cggtgcagag ggaagcaaga
     3721 tgaacgtgct actggaaccc ttcagctatg aatatatgct caatgcgatg tgggtctcgg
     3781 caatggtcgg cggtctctgc gctttcctgt catgctatct gatgctaaaa ggctggtcgc
     3841 tgattggcga tgcgctgtcg cactctattg tcccgggtgt tgcgggggca tatatgctgg
     3901 ggcttccgtt ttctctggga gccttctttt ccggcggact ggcagcgggc agcatgctgt
     3961 ttcttaacca acgcacccgc ttaaaagaag atgccatcat cggcctgatc ttctcgtcct
     4021 tttttggcct ggggctgttt atggtgtcat tgaacccgac atccgtgaac attcagacca
     4081 tcgtcctggg caatattctg gcaatcgatc cggcggatat cctgcaactc actatcattg
     4141 gcattctctc gatcatcgtg ctttttttca aatggaaaga tcttatggtg acctttttcg
     4201 acgagaatca cgcccgtgct attggattac atccgggcag attaaaaatc ctcttcttca
     4261 ccctgctgtc ggtttcgacc gttgcggcgc tgcaaacggt tggtgccttt ctggtgatct
     4321 gtctggtggt gactcccggt gcaacagcat ggttgttaac cgatcgtttt ccccgcctgc
     4381 tcataattgc tgtaactatt ggcagtgtaa ccagtttcct cggcgcgtgg gtcagttact
     4441 ttctggacgg cgcgacaggt ggaatcatcg tggtggcgca gacactgctg tttcttttag
     4501 catttgtatt tgcgcccacc catggacttt tggcaaatcg tcggcgtgcg cataaagcgc
     4561 tggaggatcg ctcatgatgg cattattact ggagccactg cagttcacct ttatgagtca
     4621 cgcactgctg atttcactgg tagtttccat accctgtgcg ctgctgtcgg tctttctggt
     4681 cctgaaaggt tgggcactga tgggtgacgc gatgagtcat gcggtttttc ctggcatcgt
     4741 gcttacctgg attctggggt tacctctggc tacaggggca ttcgtcgccg gagtattctg
     4801 cgcggtggcg acgggatacc tgaaagacaa cagccgaatc aagcaagata cagttatggg
     4861 gattgttttt tctggcatgt ttgccgcagg tctgatcttg tatattgcaa ttaaaccaga
     4921 cgtacatctt gaccatattc ttttcggcga catgctcggg ataaccatcg gcgatataat
     4981 ccagacggtg attattgccg ggctggttac acttgttatt agcgtaaaat ggcgggattt
     5041 tttgctgttc agctttgatt atcaacaggc gcaggtaagc ggtttgcata cgaggtggct
     5101 gcattatggg ctgctgtgca tggtttccct gaccattgtg gcgacgctga aagcagtagg
     5161 tatcattctc tctatttctc tgctcattgc gcctggcgcc attgcagtac tcctgacaca
     5221 acgctttcat attgcgctcc tactggcgac tggcatatcg gtaatagtgt caatgactgg
     5281 tgtctggctg tcctttttta tcgacagcgc accagctccg acgattgtcg tcttattcgc
     5341 ggtcgtgttc atcatgacgt ttaccgttac cagcatcaac gcacgcacaa agggaaacgc
     5401 ccatacacag gatctgttat cacccaatta gcacaacatc cgaggacagc atcgtccgcc
     5461 agcctacaga agtccggaaa gccatggaag gtcgttggga ggaggtacga attctatcat
     5521 gcaaaaaata cgtaaaatcg ataacgccta gaaatcattc aatactcgca ctatcgaaag
     5581 tttaccagcc aaccgcggca cgttcctgca tacgacgtgc cgcagcttct gttattccac
     5641 gataaaattc ctaaccgaat tcaaatcctt cagactattc agctcttcct tcagctc
//
LOCUS       DADLSE010000201         5686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLSE010000201 DADLSE010000000
VERSION     DADLSE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.202"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            64..972
                     /locus_tag="JN692_003815"
     CDS             64..972
                     /locus_tag="JN692_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7265979.1"
                     /translation="MKAPVSLTSFHPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN692_003816"
     CDS             complement(1018..3009)
                     /gene="tkt"
                     /locus_tag="JN692_003816"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7265980.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3287..4045
                     /locus_tag="JN692_003817"
     CDS             3287..4045
                     /locus_tag="JN692_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7265981.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVAPGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN692_003818"
     CDS             complement(4251..5171)
                     /gene="speB"
                     /locus_tag="JN692_003818"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7265982.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5307..>5686)
                     /locus_tag="JN692_003819"
     CDS             complement(5307..>5686)
                     /locus_tag="JN692_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265983.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYIVN
                     AGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1364 a         1521 c         1512 g         1289 t
ORIGIN      
        1 tgtcggctgt tttctcctca tctcatgtga catattccca tactttactc aaggaagatt
       61 gtcatgaaag cacctgtttc attaacctca tttcacccac aaaaatctct tgctatcgcc
      121 attggcgtgc ttgccgtggt tgtgctgcca tttctctcgt attacaccgt taatgaaggt
      181 gaacgcggca ttttgctgcg ctacggaaaa atcgtaaaag ttgccgagcc ggggctgggt
      241 tttaagatcc cttttatgga aagcgtggag aaaatttcca cgcgtaatca ggctgtggtc
      301 tatcagggat tacaggccta cagccgcgac cagcaaccag cacaaatgac agtatcagtc
      361 agcttccaca ttaaaccttc agaagcaggc gcggtttata ccacctacaa cactattgaa
      421 gcattaaaag atcgtctaat tgtccgtcag ttgccaactc agctggagaa cattttcggc
      481 caatataccg ccatcagcgc cgtacaggat cgtacaaaat tggttcagga tctgcaaaat
      541 gccatgcgca aagccgttgt tgggccagtg gtaattgacg gggtccagat tgaaaatatt
      601 gatttttctg acgcttatga aaagtcgatt gaagaccgaa tgaaagcaga agtcgccatt
      661 gccacgcgta agcaaaatct ggaaacagag aaaatccagg cgcaaatagc ggtaactcag
      721 gcccaggcag aagcagacag taaactggcg gcagccaaag cagaagcgga aactattcgc
      781 gtaagaggcg cggcagaggc tgaaactatt cgtttaaaaa gtgcggctga agcagaagca
      841 atccgcctgc gcggtgaagc tctgcgggat aatcccggac ttgtagccct caccactgcc
      901 gaacgttggg acggtaaact gcccgatacc atgatccccg gcagcacagt tccgtttatc
      961 tctactaagt aaaaaaaggg ccgctttcgc ggcccttctc acaaaacgtc ttactgatta
     1021 cagcagcgct tttgctttcg caacaacgtt atcaacagtg aagccgaact cttcaaacag
     1081 cagctctgcc ggagcagatt caccgaaggt ggtcatgccg acgatagcgc cgttcaggcc
     1141 aacatacttg taccagtagt cagcaatacc cgcttctacc gcaacgcgtg cagtaaccgc
     1201 tttcggcagt acggattcac ggtaagccgc atcctgcttg tcaaatgcgt cggtagacgg
     1261 catggacacc acgcgcgctt tcacgccttc ggcagtcagt ttttcgtagg cagcaaccgc
     1321 cagttcaact tcagaaccgg tggcgatgaa gatcagttcc ggctgaccag cacagtcttt
     1381 cagcacataa ccaccgcgcg cgatgtttgc cagctgctct tcagtacgtt cctgctgcgc
     1441 caggttctga cgggaaagga taagcgcagt cgggccgtcc tgacgctcaa cgccgtattt
     1501 ccacgcgacc gcggattcaa cctggtcaca cggacgccat gtagacatgt tcggggtcac
     1561 gcgcagagaa gcgacctgct ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat
     1621 ggagtcgtgg gtgtaaacca tcacctggcg ctgtttcatc agcgcagcca tacgtacggc
     1681 gttacgtgcg tattccacga acatcaggaa ggtggaggtg tacggcagga agccaccgtg
     1741 cagggagata ccgttagcaa tcgcggtcat accgaactcg cgaacaccgt agtggatgta
     1801 gttacctgca gcatcttcgt tgattgcttt agaaccagac cacagggtca ggttagacgg
     1861 tgccaggtca gcagagccgc cgaggaattc aggcaacagc gggccgaacg cttcgatagc
     1921 attctgcgac gctttacggc tggcgatttt cgccggatta gcctgcagtt tagcgataaa
     1981 ctctttcgct ttggcgtcga agtcagacgg catttcgcct ttcatacggc gggtaaattc
     2041 agccgcttcc tgcggataag ctttcgcgta agccgcaaac ttctcattcc atgcagattc
     2101 tttcgcctgg cctgcttctt tcgcatccca ctgagcatag atttcagacg ggatttcgaa
     2161 cggtgcatat ttccagccca gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg
     2221 cgcaccgtgg gagtcgtgag taccggcttt gttcggggaa ccgaaaccga tgatggtttt
     2281 gcacatcagc agggacggtt tgtcagtcac tgcgcgcgct tcttcaactg cgcgtttgat
     2341 ggatgccgcg tcatgaccgt cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg
     2401 cattgctgtg tcgtcggtga accagccttc aacgtgacca tcgatagaaa taccgttgtc
     2461 atcgtagaac gcaatcagtt tacccagctt cagcgtaccc gccagagagc aaacttcgtg
     2521 ggagatgcct tccatcatgc agccgtcgcc catgaaggcg taggtgtagt ggtcgacaat
     2581 gtcgtggccc ggacggttaa actgcgccgc cagcgttttt tctgcaatcg ccataccgac
     2641 tgcgttggca ataccctgac ccagcggacc ggtggtggtt tccacaccag cggtataacc
     2701 tacttccggg tggcccggag ttttagagtg cagctgacgg aagtttttca gttcttccat
     2761 cggcagatcg taaccggtga ggtgcagcag gctgtagatc agcatggagc cgtggccgtt
     2821 ggacagcacg aagcggtcac ggtcagccca ggacggattc tgcgggttgt gtttcaggaa
     2881 atcacgccac aggacttcgg caatgtcagc catacccata ggggcacccg ggtgaccgga
     2941 tttggctttc tgtactgcgt ccatgctcag cgcacgaata gcattggcaa gctctttacg
     3001 tgaggacatt ttgactccag atcggatgat gaagggcacg cccttaacga cttgacgaca
     3061 gcgcgttttg ggctacgccg gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt
     3121 acatggaaca tccttttgcc gcttcagaaa tctctggatc atgctcgcat gttgcgcaat
     3181 ctgctcgccc gtccgctgcg cttttcctta tactgagact gagcgtcgat tcacctgcaa
     3241 acggcgcatt tttagaataa tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc
     3301 cttattggta gcaatgagcg tggcaacggt actgaccggt tgccagaata tggactccaa
     3361 cggactgctc tcatcaggag cggaagcttt tcaggcttac agtttgagtg atgcgcaggt
     3421 gaaaaccctg agcgatcagg catgtcagga gatggacagc aaggcgacga ttgcgccagc
     3481 caatagcgaa tacgctaaac gtctgacaac tattgccaat gcgctaggca acaatatcaa
     3541 cggtcagccg gtgaattaca aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc
     3601 taacggctgt atccgcgtct atagcgggct gatggatatg atgacagata acgaagtcga
     3661 agcggtgatc ggtcacgaga tggggcacgt ggcgttaggc catgtgaaga aagggatgca
     3721 ggtggcgcct ggtacaaatg ccgtgcgagt agctgcggcc tctgcgggcg ggattgtcgg
     3781 cagtttatct caatcacaac ttggtgatct gggcgagaaa ttagtcaatt cgcaattctc
     3841 ccagcgccag gaagcagaag ccgatgatta ttcttacgat cttctgcgcc aacgcggcat
     3901 cagcccggca ggtcttgcca ccagctttga aaaactggcg aagctggaag aaggccgtca
     3961 aagctcaatg tttgacgacc atcctgcatc cgccgaacgt gcccagcata ttcgcgatcg
     4021 catcagcgcg gatgggatta agtaaagcct ggtggtgtcg aacgtaggtc agataaggcg
     4081 ttcacgccgc atctgacatg aatggcacat ttgttacctt gtacgcattg ccggatgcga
     4141 tgctggcgca tcttatccgg cctacgggtg ccgaacgtag gtcggataag gcgttcacgc
     4201 cgcatccgac attaatggca cgttttaccc gtgcgcatcg catctggtgc ttactcgccc
     4261 tttttcgccg cctgaatata cagcatctcc agcgccagcg tcgccgcagc cagagcggtg
     4321 atttccgact gatcgtatgc cggagccact tccactacgt ccatcccaac gatgttgaga
     4381 tctttcaggc cgcgtaccag tttaatggcg cgatcggagg tcaggccgcc aatcactggc
     4441 gtaccggttc ccggcgcgaa agcaggatcc aggcagtcga tatcaaaagt caggtaaacc
     4501 ggcatatcac ccacaatctg tttcacctgg gcgataacgt catccacgct gcgatcgttc
     4561 acctggcagg cgtccagcac ggtaaagccg ttgtctttat caaactcggt acgaatacca
     4621 atctgcacgg aatgattcgg gtcgatcaga ccttctttcg gcgcggtata gaacatagtg
     4681 ccgtggtcaa attcacaacc gttcgcatag gtatcggtgt gggcgtcaaa gtgtaccagc
     4741 gccattttgc cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac aaagtggtca
     4801 ccaccgaagg agagcatacg cttaccggca gccagcagct tctcggcgtg cgcctgcagc
     4861 ttttcgctca tctcacgggc atcgccaaag gcatatacca gatcgccgca gtccacgacg
     4921 ttcagacgct cacgcatgtc gaaattccac gggaagcggt tgtgttccca ggccagattc
     4981 gtcgaaacct gacggatcgc tgccggaccg tggcgaccac ccgcacgacc agaagtggcc
     5041 atatcgaacg gcacgccagt aatcacccag tctgcatcgc tgtcatacgg ctggaagttc
     5101 atcggcaggc gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta ttgatgacct
     5161 aaggtgctca tggatggact cctattacaa agtcgatata aaaaaaccct tccgcgtcgt
     5221 taggcccgac gaggaagggt tggatttgcg aaacacaagt ttgtgctaat tatcgcctta
     5281 aatgggcgag gcatcaagcg aggttgctat ttccgacacg gtggcggcgc atagccattg
     5341 ttatctgccg gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc cgggccttgt
     5401 aattcaaagg cggcaaagcc gattaccccg gtattacgca catcgccttg cgccgaattg
     5461 gctgcgtagg aatcagcaac gttggcgaac tgaaaagccg cttcagtatg tttatctttg
     5521 cggaatcctt taatcgctaa cgaatctccg gcattcacaa tatagccgtt gttattcagc
     5581 gaaccttgct tgccgttcag cacatccagg ccatcaaccg tagcaacgat ctcgtagttg
     5641 gtgttacgac tgtaattacg gacatataat tgataattca ttccga
//
LOCUS       DADLSE010000202         5675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLSE010000202 DADLSE010000000
VERSION     DADLSE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.203"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..785
                     /gene="msrP"
                     /locus_tag="JN692_003820"
     CDS             <1..785
                     /gene="msrP"
                     /locus_tag="JN692_003820"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HAY7265984.1"
                     /translation="NLPLTPADKVSGYNNFYEFGLDKDDPAANAGSLKTDPWTLKISG
                     EVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAEPTSNAKY
                     VAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNG
                     APVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQ
                     ATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
     gene            786..1421
                     /gene="msrQ"
                     /locus_tag="JN692_003821"
     CDS             786..1421
                     /gene="msrQ"
                     /locus_tag="JN692_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HAY7265985.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            1679..2329
                     /gene="zinT"
                     /locus_tag="JN692_003822"
     CDS             1679..2329
                     /gene="zinT"
                     /locus_tag="JN692_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7265986.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2999..3088)
                     /locus_tag="JN692_003823"
     tRNA            complement(2999..3088)
                     /locus_tag="JN692_003823"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Ser,seq:cga)
     gene            3182..3979
                     /gene="mtfA"
                     /locus_tag="JN692_003824"
     CDS             3182..3979
                     /gene="mtfA"
                     /locus_tag="JN692_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7265987.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEIAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4080..4155
                     /locus_tag="JN692_003825"
     tRNA            4080..4155
                     /locus_tag="JN692_003825"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4113..4115,aa:Asn,seq:gtt)
     gene            4317..5579
                     /locus_tag="JN692_003826"
     CDS             4317..5579
                     /locus_tag="JN692_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7265988.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1504 a         1328 c         1311 g         1532 t
ORIGIN      
        1 ataacctgcc actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg
       61 ggctggataa agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac
      121 tgaaaatcag cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc
      181 gcttcccgct ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg
      241 tgccgtggat tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg
      301 cgaagtatgt cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc
      361 gctttatcgg cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa
      421 tgcatccgct cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg
      481 gcgcgccggt gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg
      541 tcagtattaa gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg
      601 aatacggttt ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta
      661 ccgaacgatt tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt
      721 ttaatggtta cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt
      781 tctaaatgcg tctgacagca aaacaggtga tatggctgaa agtttgcctg catcttgccg
      841 gattgttgcc gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc
      901 cggtgaaaga tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct
      961 tgttaatcac acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc
     1021 tgttaggctt atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg
     1081 agttgggcgt gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa
     1141 cgttaggaat tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga
     1201 tgcagcgaaa actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga
     1261 tcctggcccc aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct
     1321 acgctgggct ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca
     1381 accggttacg caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac
     1441 ttcgcgttat cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc
     1501 aagttatgtt tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata
     1561 agatagttca atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt
     1621 ttcatatgtt acaatataac attacacata atgtacatta actctggagg aaattgtttt
     1681 ggcgattcgt cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc
     1741 cttttcgcat ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc
     1801 taatggtgtt tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt
     1861 ctggcaatcc gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa
     1921 agcggatgca gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta
     1981 tccgacagat atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa
     2041 tgaaacaaca tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg
     2101 caagaaaggc gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata
     2161 tatacaattt agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat
     2221 gggtaatgat tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc
     2281 ctatcagttg agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc
     2341 tgacttcagt tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa
     2401 ataagtaaga caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt
     2461 agaaaaaaac tgagaaaaag gcgaacaata aatacacaac tcagccatac agactggcag
     2521 cgcaaatgtt tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta
     2581 attttggtgt ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg
     2641 ggaaattcaa caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg
     2701 cgttcctttt ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca
     2761 actttgcggc agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac
     2821 gactgaataa ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc
     2881 gctgaatgtc acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta
     2941 cgattgttct gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaagtg
     3001 gcggagagag ggggatttga acccccggta gagttgcccc tactccggtt ttcgagactg
     3061 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
     3121 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
     3181 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
     3241 ggaagcacta tcgatccccc ttttaacgtg tctgacagaa caggaacaaa gcaaattggt
     3301 cgctcttgcc gaacgttttt tacagcaaaa acggcttgtt cctttacagg gctttgagct
     3361 gaattcatta agaagctgcc ggatagcact tctattttgc ctgcccgttc tggagttagg
     3421 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
     3481 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
     3541 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
     3601 ttctggtttt aacctgatta ttcatgaaat cgctcataag ctggacaccc gtaacggcga
     3661 tcgcgccagc ggagttccct ttatttcgtt gcgtgaggtt gctggctggg aacacgatct
     3721 tcatgctgca atgaacaaca ttcaggaaga aatcgaatta gttggtgaga atgcggcgag
     3781 cattgatgct tatgctgcca gtgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     3841 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgtca
     3901 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     3961 ggcgacgaat gttcattaat taacaacttt gcagattaat taaccaattg aaatggctta
     4021 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tctcacgatt
     4081 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     4141 cagtcagagg agccaatttt ctgttttcat gcatccttgc gaatctttat ctgatgttga
     4201 ttcaacaggt tagtgtgaaa actcttctcg gtgcattttg attttaccct acgcatcggg
     4261 aaaaattggt ggtcaaatct ggggtcaggt tagttcgata atggagtgac ccccatatat
     4321 cccttaccga cgcaaaaatc cgcaccctca agccttctga taaacccttt aaagtctccg
     4381 attctcacgg tctgtatctg ctggtcaagc cgggtggctc ccgccactgg tatctcaaat
     4441 accgtattag cggtaaagaa tcccgcattg cgctgggtgc ctatccagcc atctccctgt
     4501 ctgatgcgcg acagcaacgt gaaggtatcc gtaaaatgct ggcgctgaat atcaacccgg
     4561 tacagcagcg ggctgctgaa cgtggctcac gaacaccgga gaaagttttt aaaaacgtgg
     4621 cgctggcgtg gcataaaagt aacaggaaat ggtcgcagaa caccgccgac cgtctgcttg
     4681 ccagcctgaa caatcacatc tttccggtca tcgggaacct acctgtatca gaacttaaac
     4741 cccgtcattt cattgacctg ctgaaaggga tcgaggaaaa aggtctgctg gaggttgcgt
     4801 cccgcacacg gcagcacctg agtaacataa tgcgccatgc ggtccatcag gagttaatcg
     4861 atacgaaccc tgcagcaaac cttggcggcg tgaccacacc tcctgtcaga cggcactatc
     4921 ctgccctgcc gctggagcgg ctgcctgaac tgcttgaacg tattggggca tatcatcagg
     4981 gccgtgaact gacccggcat gccgttctgc tgatgctgca tgtgttcatt cgctccagtg
     5041 aactgcgttt cgcccgctgg tcagagattg atttcacaaa ccgagtctgg acgatacccg
     5101 cgacgcgaga acccattatt ggcgtgcgtt attccggccg cggggcaaaa atgcgaatgc
     5161 cgcatatcgt ccccctctca gaacagtcca tcgccattct gaaacagatt aaggatatca
     5221 ccggtaataa tgaactgatc ttccccggcg accataaccc gtataagcca atgtgtgaaa
     5281 acacggtcaa taaggcactg cgggtgatgg gttacgacac gaaaaaggat atctgcggtc
     5341 acggcttccg ggcaatggca tgcagtgcgc tgatggaatc gggtttatgg gcaaaggacg
     5401 cagtagaacg ccagatgagt catcaggagc gcaataccgt gcgcatggct tatattcata
     5461 aggcagagca cctggaagcc cgcaaagcga tgatgcagtg gtggtcggat tatctggaag
     5521 catgccgaga atcttatgca ccgccttata caattggtaa aaataagttt atcccatagt
     5581 atactaacga atcccacact ctggcaaatt accaccacct ctacaggagg tggttctata
     5641 tagactttta tcaacttcat gacgaaaaac accta
//
LOCUS       DADLSE010000203         5672 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLSE010000203 DADLSE010000000
VERSION     DADLSE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.204"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..979
                     /locus_tag="JN692_003827"
     CDS             <1..979
                     /locus_tag="JN692_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HAY7265989.1"
                     /translation="CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQL
                     VAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSR
                     ASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPF
                     IGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGL
                     FLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAAALGFISAIGAV
                     GGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1061..4801
                     /locus_tag="JN692_003828"
     CDS             1061..4801
                     /locus_tag="JN692_003828"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7265990.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFQEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            4798..>5672
                     /gene="narH"
                     /locus_tag="JN692_003829"
     CDS             4798..>5672
                     /gene="narH"
                     /locus_tag="JN692_003829"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7265991.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSSPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         1327 a         1530 c         1549 g         1266 t
ORIGIN      
        1 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
       61 aaaagccaaa caagggagtg ctcttgggat taatggcgga ttaggaaact taggtgtaag
      121 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
      181 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
      241 ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag
      301 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
      361 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
      421 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
      481 tattggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg
      541 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
      601 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
      661 gaccgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
      721 aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt
      781 caccgaaacg gcggcagcgc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
      841 tattccgcag gcatttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
      901 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
      961 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     1021 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     1081 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     1141 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     1201 ttccacccac ggcgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     1261 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     1321 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     1381 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagcactcaa
     1441 acaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     1501 gagctacaaa caagtgcgtg ggcgcggcgg gttcatccgc tccaactggc aggaactaaa
     1561 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     1621 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     1681 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     1741 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     1801 cagctacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     1861 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     1921 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     1981 gatggcaatg gggcatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     2041 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     2101 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     2161 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     2221 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     2281 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     2341 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     2401 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     2461 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     2521 ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     2581 tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg aatttgccga
     2641 taccgcccat aaaacgcatg ggcgctcaat gattatcctc ggcgcaggtg ttaaccactg
     2701 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     2761 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     2821 cggctggttg ccactggcct ttgcgctcga ctggaaccga ccaccgcgcc agatgaacag
     2881 cacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     2941 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     3001 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     3061 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     3121 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     3181 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     3241 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     3301 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     3361 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     3421 caccgccacc tggtatgaaa aagacgatat gaacacctcg gatatgcatc catttattca
     3481 tccgctttct gcggcggtcg atccggcctg ggaatcacgc agcgactggg aaatctacaa
     3541 aggtattgcc aaagcatttt cgcaagtgtg cgtgggccat cttggcaaag aaaccgacgt
     3601 ggtattacaa ccactgctgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     3661 cgactggcgc aaaggcgaat gcgatctgat cccgggtaaa accgcgccga atattgtggc
     3721 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     3781 caaacttggc aatggcggta aagggatttc gtggaatacc caggatgaaa tcgatttcct
     3841 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     3901 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     3961 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     4021 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     4081 ctccagcccc acatggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     4141 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     4201 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     4261 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttccaggaaa aagcacttaa
     4321 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     4381 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     4441 gaccattgtc gataacgact gggtggaagt atttaacgcc aacggcgcgc tgactgcccg
     4501 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     4561 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     4621 cacccgcgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc tggcctgggg
     4681 ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga tccgcaagat
     4741 gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     4801 aaaatacgtt cgcaagtcgg catggtgctt aacctcgata aatgtatcgg ctgccatacc
     4861 tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta cgcatggttt
     4921 aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag
     4981 tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag
     5041 atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagcagat tgatgattac
     5101 tacgaacctt tcaccttcga ctacgaacat ttgcatagct caccggaagg caaacatatc
     5161 cctactgctc gcccgcgttc actgattgac ggcaaacgga tggacaaagt gatctggggc
     5221 ccaaactggg aagaactgct gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc
     5281 gaggccatgc aaaaggagat gtacgggcag tttgaaaaca ccttcatgat gtacctgccg
     5341 cgcctgtgcg aacactgcct caatcccagc tgcgtggcga cctgcccaag cggcgctatc
     5401 tacaaacgcg aagaagacgg cattgtgctg attgatcagg ataaatgccg tggctggcgt
     5461 ttgtgcatta gcggttgtcc gtacaaaaaa atctacttca actggaaaag cggcaagtca
     5521 gaaaaatgta tcttctgtta tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa
     5581 acctgcgtgg gtcgcatccg gtatctgggc gtgctgcttt acgacgccga ccgcatcgag
     5641 gaagcggcga gcaccgagcg cgaagttgac ct
//
LOCUS       DADLSE010000204         5648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLSE010000204 DADLSE010000000
VERSION     DADLSE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.205"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            193..294
                     /locus_tag="JN692_003830"
                     /pseudo
     CDS             193..294
                     /locus_tag="JN692_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(289..705)
                     /locus_tag="JN692_003831"
                     /pseudo
     CDS             complement(289..705)
                     /locus_tag="JN692_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(750..1184)
                     /locus_tag="JN692_003832"
                     /pseudo
     CDS             complement(750..1184)
                     /locus_tag="JN692_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1196..1417)
                     /locus_tag="JN692_003833"
     CDS             complement(1196..1417)
                     /locus_tag="JN692_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265992.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1418..2101)
                     /locus_tag="JN692_003834"
     CDS             complement(1418..2101)
                     /locus_tag="JN692_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7265993.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2165..2476)
                     /locus_tag="JN692_003835"
     CDS             complement(2165..2476)
                     /locus_tag="JN692_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7265994.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2473..3444)
                     /locus_tag="JN692_003836"
     CDS             complement(2473..3444)
                     /locus_tag="JN692_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7265995.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFSGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLKVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            3815..4774
                     /locus_tag="JN692_003837"
     CDS             3815..4774
                     /locus_tag="JN692_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7265996.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAIIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQLALVKAMVHA"
     gene            4774..5169
                     /locus_tag="JN692_003838"
     CDS             4774..5169
                     /locus_tag="JN692_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7265997.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5352..>5648
                     /locus_tag="JN692_003839"
     CDS             5352..>5648
                     /locus_tag="JN692_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7265998.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRR"
BASE COUNT         1554 a         1475 c         1364 g         1255 t
ORIGIN      
        1 acacgacgct gaggctacag agctgaaaac ccacaaggcc atgccccgca tggccgggta
       61 aagcggagcc ggcggcgact tcctttgtgc tctgtccgag accggcagga aaaaggcggg
      121 gataatgaga tggtcacccc caccctaaca gcagtacgct gttaaggtga atcccttaac
      181 ggagagacca tcatacagaa catcacactt attggtatcg atctcggaaa gcattccttc
      241 cacattcatt gtcaagacaa gtctggtaag gctcttctgc gtaaaaaaga tacgccgcgc
      301 atcttctgcc ccccggcaac tgacactgca tactgcatcg cgcaccgcat caagaactgc
      361 cctgatttcg tcttcggtca gccattcacc ggcatacggg aaagacacct gcgttccgtc
      421 cgccggaagt accggcagcg actgccactg actgccggac agaacaagtt ccgctccggt
      481 tccggtcagt ttctgaatgg cggaggccag gtgcggcggc agcacgccat cgtggcggtt
      541 aacgagtaac gtcgccagcc ggggcgcatc cagccgcacg taaatgctga tccagcccgg
      601 atagttcggg ttacgttggt caacatccgt ctcttccggc cagccctgcg ccagcagcag
      661 gttacgggcg tgacgacaaa tcagagcatt cagcgtttca ttcataattc atgaactccc
      721 tgtatcgccg cccgcaggcg gcgataacat cagtcgtttt cgtgtgtgtt gtttttcagc
      781 aggctgcggg cctgatcaac catcgcctgt cggcgggtaa cgtattccgg gagggggaaa
      841 ccgtgatccc gggcagccca ttcacgtttc cacagtgcct gttcagcgat ctgagtcagg
      901 gtctccagca gttcagcagg gacggtcacc tggcggtttt cgcgcacgtt gctgataatc
      961 tccatcgccg cggcggaaga aataccggac tcaatccgct agtcttccgg gatgtcctcc
     1021 aggtgcgcca ggacggtcct gatttcatga tcggacaggg aaatatccat tccgtcagcg
     1081 agggcctgaa tctcttccgg ggaccagata accagcatca gcggcgtgtc tcccgaatac
     1141 tttgctgcca gcgcccggca tagcgtttcg catgttccgt acatacactc tcccctcaat
     1201 ctttgaagca ggaatccagt tccagaattt cggtatatgc ctccatcaag aggcggtttg
     1261 cgcgaaacaa cgtctgacgc accagacgtt ccggcagggt gtcagcatta aagctgtacc
     1321 gccgctccat ctcattcatg gttgtacgca ggtcgcacat ggcattggcc acctccgcca
     1381 cttcggcgcg aagtttttcg tgtccggcat agttcattta cgcagcctcc ggcataaacc
     1441 agtcatcatt cgcggccccc tgctgcatgg cccgctgtac ggcagccagt cgttgctgtc
     1501 cggcacggtg atactgctca agcagctcga taccgatata ccggcgtccg gactggaggg
     1561 cggcgacgca ggttgaaccg ctgcctgcaa acgggtccag cactatcgca ttcgggtgtg
     1621 tgaagctctc aatcagcggt tgcaggctgg taacgggctt ttccgtcggg tgatggcggt
     1681 tgcccgaata tttccagccc aacacgtccg gcagcgggtt ttgtggcaga cgtggacggc
     1741 cttttgccag gatgtaggcg cattcgtggc gatagcccac atatgcggcc ttcgatgtgt
     1801 agtttttggt gaagaccagg tgaccaacaa cgctgaatcc cgcatttttc caggcagaca
     1861 taaagcgatc gacgcggttc cagccataga agctcaccat cagcgcgtct tttttgagta
     1921 cgcggtacat ttcattacag gccggttgca gccattcatc agttttatca ccagcaatgg
     1981 tacgcccctg acggtcacga aaaccgacga gatatggcgg atcggtgagg atgaaatcaa
     2041 tggcgttatc aggaatacgg gccataacat cgatgcagtt accgaggaca aaacgggaca
     2101 taacagatcc ttctcttaca gagggcaccc ctgtggtgcc cgttccccgc tgcctgtggg
     2161 ctgttcaccg cgtccggtaa cgggtcaggc cgaccgtgga ataccgcagc cgtcgtcttt
     2221 ttgacagcaa ggttatgccg cgaaagcggc ttgaagggcg ttcatcagaa aagggcgacg
     2281 ggctggcgta cactgtcacc gacgccagcc gggagcggta aaagcgcccg tcccgtgccc
     2341 gccggacgcg ggacactgac cacaggcagc gtttatgccg cgccgcgtca gccggcgcag
     2401 taaaaagagg agcacggcga catcctttgt cggaaaagaa acgggcggtt gcccgcccgt
     2461 ctccgtcata cttcatccgc cataatgcgc cacattgtcg actatccacg caggtccggt
     2521 gacttccggc tgctcatcgt catctgtcgg ggaagaccat tcaagttccc cccacagcac
     2581 atcgacgagc tctccacgct catacaactg ccagccacag aaatcacagc cctgttcggc
     2641 ataccagtgc tccagcgtgc agctgaaacg gcggcttaat tctgcaataa cgtcgctttc
     2701 cggctgacac cacggcgtgt cgaaatccac ctgaatgaag ttttctccct gactgctgat
     2761 attcaggtca tatccacaag gccacttcac gccataccgt tccgtatacc agtgatatgc
     2821 agaaggaaca ccgttcagaa caccaccatt aaagccgttc acttcaacat ccaggcgggt
     2881 cggcagaacc ttcagcaggt caaacggcat ggttttttcc ggcactacgt tgtcacacag
     2941 acggttccac cacaccgata catcctcgtt accccatatg tcacaccagt cacctctttt
     3001 tgcggtgaaa tgcacagtga tggtttccct ggcatcatcc ggtaatcctt cccagcgacg
     3061 ctggccagta ccgctctgta accagagttc atgcagcatc aggcagtttt gttcatccag
     3121 caacacaccg ttctgcaggt gtgtcagcca gcgggtgaac gcgatatttt ccgtcgatac
     3181 agcaccacgt ccggcagccg tcactccggg gcactgttca gagcggatat tttcagtgat
     3241 ctgcagaagt ccggcacttc cggccagaaa cagctgaata ccttcatttg tggcgcggcg
     3301 ataaagcggt gtgactgcac cgctggccag tcgtttaatc tcagcgatct gtgccggttc
     3361 accagaaaaa tacatacgat tcgagcacca gtttggcatg ggaattctcc tctcacatgc
     3421 ggcctccatc aggccgtgcc gcacctgtaa ccgggcgacc gcatacgaat gcaggtgcgg
     3481 ccccgaagcc gcagcgcgtg gaggaggaac gacgaaacgt gaccgggtga ggaggccggc
     3541 gacagcgaca cacttgcatc ggatgcagcc cggttaacgt gccggcacgg cctggttaag
     3601 tagtatccgt ctcagttcgt ggcgcaaaat gagaaaccaa taagaactca aaatgaacca
     3661 caacaagaac ataaagagac tctaaaagag caacaccgct tgacatagac tcaaaatgag
     3721 tcacaataag aaacaataaa aaactcaaaa agatgcaccg acataaagga tgctcaattt
     3781 gagataaaca caaactcaaa acgaggcaaa aagaatgcta aaagtatcat gcgatgatgg
     3841 ttcaactaac gtaaaactgg cctggcttga agatggcgag gtaagaacca gtctgtcagg
     3901 aaacagcttt aaagaaggct ggaacccggg actgtttaat gccggcaaag tttacaatta
     3961 cgtcgtggat gaaaaaaaat acacctatga tttgggaagc actgctatca taggtacaac
     4021 acatgtcagt tatcaataca gcaccaccaa tctgcttgcc atacatcatg cactgctgac
     4081 atccggcctt cagccacagg atgtagaact gacagtaaca ttacctgtga ccgaattctt
     4141 tgataatgac aatcaaccaa atgaagaaag aatagaaaga aaaaaggcaa atgttctccg
     4201 tgagatatct ctgaataaag gggaaacatt taaaattaaa aaagttaatg taatgcctga
     4261 atctttgcca gctgcatttg aatcactaaa aaaagataaa gtaaacaaac ttgaacgcag
     4321 cctgattatc gatcttggag gaaccactct ggattgtggt ttaattctcg gtgcatttga
     4381 aggcatttct gaaatccgtg gctattcaga aatcgggaca tcccgcatca ctcataccgt
     4441 catgaatgcg ctgacaaaag cctccacacc atgtaattat tttattgctg atgagttaat
     4501 aaaaaacaga cacgataatg aatatctcca gacattaata aatgatgtag ctgaaataaa
     4561 aaacatttct catgtaatag acagagaagt gaaatctctg gcagaaagca tcaggcagga
     4621 aatttcaaca ttctcaggaa tgaaccgaat ctatctcact ggcggaggag ccgagcttat
     4681 ttaccctcac ataaagcaat atttcccgaa tctgaaagtg aacaaggtgg atgaacctca
     4741 gttggcattg gtgaaagcaa tggtgcatgc gtaatggaaa gcagtgatcc aaaaaaaaga
     4801 aagaaagttg tggcatacct gcacccggca ctttatccgc aggacaacct gacacaacaa
     4861 acaatagact ctctcccagt acaaatgcgg ggagatttct acagacaatc ccttatttgc
     4921 ggagcagctc tttattctgt tgacccccga ctgttaacgc taatcagcgg ttttttcagt
     4981 gagaaaatca ctgcagaaaa tctggtgaaa cttatagaac aaacaacagg atacacatcc
     5041 acatcaattg atataagtgt gttaaaaaac atcatagagg catcttcaga aaacaaatca
     5101 gagagcatca cttcaaaaga tgatttcgaa gaacaaacca gacgcaatct ttctatgtta
     5161 aaaaaataat aaaagaagag caggttcagt caattacctg ctcttctttt atacatacta
     5221 aaaaatcaga tttttacgaa tattcagatt atcataaaaa atgcaacaga caggaatgca
     5281 ataattttgc attatctcgt tgcttgtaat gcaaatatgc attattgtca gtgtgtgaca
     5341 ataacagaga gatgacgatg gcgaccatta acgcacgtat tgatgatgat ataaagaacc
     5401 aagcagatga agtgctgaag ctgatgaata tcagtcaaac tcaggctatt gcagcttttt
     5461 atcagtatat tactgaacaa aagaaactgc catttgtgat cacctcaatt gtaaaaacgc
     5521 cgcatgattt attacgtgaa agtacagaca tgttagctga agctcttgca gtgatttcca
     5581 atctacaggt gtggactgaa cagcaggatg ggattggaaa agcaaagcta atggagtatt
     5641 accgccgc
//
LOCUS       DADLSE010000205         5611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLSE010000205 DADLSE010000000
VERSION     DADLSE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.206"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN692_003840"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN692_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7265999.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JN692_003841"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JN692_003841"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7266000.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JN692_003842"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JN692_003842"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7266001.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JN692_003843"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JN692_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7266002.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1392 a         1351 c         1524 g         1344 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatattttgc ctgaaaagtg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta acgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg g
//
LOCUS       DADLSE010000206         5586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLSE010000206 DADLSE010000000
VERSION     DADLSE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.207"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..200)
                     /locus_tag="JN692_003844"
     CDS             complement(<1..200)
                     /locus_tag="JN692_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HAY7266003.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLS"
     gene            complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN692_003845"
     CDS             complement(487..1212)
                     /gene="phoU"
                     /locus_tag="JN692_003845"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7266004.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN692_003846"
     CDS             complement(1227..2000)
                     /gene="pstB"
                     /locus_tag="JN692_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7266005.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN692_003847"
     CDS             complement(2092..2982)
                     /gene="pstA"
                     /locus_tag="JN692_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7266006.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN692_003848"
     CDS             complement(2982..3941)
                     /gene="pstC"
                     /locus_tag="JN692_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7266007.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN692_003849"
     CDS             complement(4028..5068)
                     /gene="pstS"
                     /locus_tag="JN692_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7266008.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5316..>5586)
                     /locus_tag="JN692_003850"
     CDS             complement(5316..>5586)
                     /locus_tag="JN692_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266009.1"
                     /translation="TSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTD
                     MGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         1384 a         1475 c         1353 g         1374 t
ORIGIN      
        1 tgacttaaga gttcgcttaa tcgcccgttc agttcatgac tgctcaaggc atactctttt
       61 tctattccac ttgagttaat tctttcgcca gcgcgttttt gaaagccaat tccgcgcccc
      121 atgacgactt tttcccgctg ttgatcatca ataaccacca caacattatt gttgagaatt
      181 ttggtgattt gcatgttcat agcaaggacc ttttttataa acaaaaaaaa cccgacttca
      241 ccagtattct ctggttatgt caggttttgc ctgcgaatgc agtaacaatc cagtcattta
      301 ttaatggttt ttataacgaa catccaggtt cggaaattaa tttaattgcg tgcagagaaa
      361 atagcaatgc gctattgata aaaatatgac catgctcgca gttattaact ttgtgtaatt
      421 ttaggaattt ataaagttat atataacaaa tcccaataat taagttattg ggatttgtct
      481 ggtgaattat ttgtcgctat cttttcccgc cagcagttta tccagctcat caccgcctac
      541 atgacggaaa tcctgaccct tcacgtagta gaagataaac tcacaaatat tctggcagcg
      601 gtcgccgata cgttcgattg aacgcgcaca gaacagcgcg gtaagtacgc tcggaatagt
      661 gcgcgaatct tccatcatgt aggtcatcag ttgacgaaca ataccttcgt attcctgatc
      721 gactttttta tcttcacgat aaatacgtac cgcttcgtca atgtccatcc gcgcgaacgc
      781 gtccagcacg tcgtgcagca tctggatggt atgacggccc agcgactcca gacttaccag
      841 caacggctga tgctgctggg agaatttctc cagcgcagta cggcagattt tgtccgccac
      901 gtcgccaata cgctccagct cggcaatggt tttactgatc accataacca gtcgcaggtc
      961 gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag gcttcatcga tcgccacttc
     1021 catcatgttg acgttcttgt cgccttcgat cacgcgcttc gccagatcgc tgtcctggtt
     1081 atgcatcgcg gtgattgcat cagaaagctg ctgctccacc atgccgccca tggtcatcac
     1141 ctgcgtgcgg atactttcca gttcggcgtt gaactggccg gaaatatgtt tattaagatt
     1201 gagactgtcc ataacgcact cctgaatcaa ccgtaacgac cggtgatgta gtcttctgtt
     1261 tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc tgaactcaat caattcgccc
     1321 aagtacataa atgccgtgtg gtcggaacaa cgcgcagcct gctgcatgtt gtgggtgacg
     1381 atcaccacgg tgtaatcctg cttcagttcg gtgatcagct cttcaatacg cccggtagag
     1441 atggggtcga gcgccgaaca cggttcgtcg agcagcagca cttccgggcg aatggcgata
     1501 ccacgcgcaa tacacagacg ctgttgctga ccaccagaga gagagtaacc gctctggtgc
     1561 aatttatctt tggtttcgtt ccacaatgcg gctttggtca atgcccactg cacgcgctcg
     1621 tccatgtcgg cacgggagag cttctcaaac agacgaacgc caaaggcgat gttgtcgtag
     1681 atggacatcg ggaacggcgt cggtttctgg aacaccatgc ccactttcgc acgcagcagg
     1741 gcgatatcct gagagttggt caggatgttg tcgccatcaa gcagaatttc accttccgca
     1801 cgctgctccg ggtacagttc aaacattttg ttgaaggtac gcagcagcgt cgatttaccg
     1861 cagccggacg gcccgataaa cgccgtaacc tggtttttgg cgatatccag gttgatgttt
     1921 ttcagggcat ggaatttgcc gtagtagaag ttcaaattac gaacctgaat tttactcggg
     1981 gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt acctgatgct tttcgctaat
     2041 caggcttacc gtagggccgg gtaaggcgtt cacgccgcat ccggcaaagt gttaaccgtg
     2101 tttattcttc gcaaaaacaa cgcgcgccag aatgttcagc agcaggacgc acagggtaat
     2161 gatcaatacc ccggcccagg ccagttgttg ccattccgca aacgggctca tcgcaaactt
     2221 aaagatcgtc accggcaggt tggcgatcgg ctgcatcatg tccgtgctcc agaactggtt
     2281 ggagagcgcg gtaaacagca gcggcgcggt ttcaccggca atacgggcaa tcgccagcag
     2341 gataccggtc ataatcccgg acaccgacgc tttcagcgta atcgcggaga tcatcttcca
     2401 cttcggtgta cccagcgcat aagccgcttc acgcaggctg tccggcacca gtttcagcat
     2461 gttctcggtg gtgcggataa cgatcggcac ctgcaacagc gccagcgcaa ttacgcccgc
     2521 ccagccggag aagtgctcca tctgtgccac cacgatggtg tagacaaaca gaccaaccac
     2581 aatcgacggc gcggagagca gaatgtcgtt aatgaagcga atcacttctg ccagccagga
     2641 tttacgacca tattccgcca gataaatccc cgccataatg cccagcggcg taccgaatac
     2701 cgtggcccac aaaattaaca gcccgctacc cgccagggcg ttcgccagac caccaccttc
     2761 cgtattaggc ggcggcgtca tttcagtgaa cagcgccagc gacataccgt cgataccgcg
     2821 agtgatggtg gacattaaaa tccagatcag ccagaacagg ccgaaggcca tcgtcgccat
     2881 cgagagcgtc agcgcaatac ggtttttgag gcggcgacgc gcctgcattt tgcggcgaga
     2941 ttcagccagc gccgcagtgg tttgcatttc aaccatagcc attagcgtgc cccctcattc
     3001 ttagccaggc gcataatcat aaacttcgat gcggcgagga cgataaaggt aatcacaaac
     3061 aggatcaggc ccagttccat cagtgcggca acgtgcagac cggattccgc ttccgcaaat
     3121 tcgttcgcca gcgcagaggt gatactattg cccggcatgt acagcgaggc gctgtcgagc
     3181 tggtaggtgt taccgatgat aaaggtcacc gccatggttt caccgagcgc gcggcccagc
     3241 cccagcatga tgccgccgat aacaccattt ttggtgaacg gaagaacgat acgccagata
     3301 acttcccagg tggtgcagcc aataccgtag gccgactctt tcatcatcac cggggtttgt
     3361 tcgaacacat cacgcattac cgccgcaatg tacggaataa tcatgatggc gaggatcacg
     3421 cctgccgcga ggataccgat accaaatgcg gggccagaga acagcgcgcc aacaatcggg
     3481 atattcgaca tgatattgcc gaccggctcc tgaaagtaaa cggcgaacag cggcgcaaag
     3541 ataaacaggc cccacatgcc gtaaacgata cttggaatgg ctgccagcag ctcaatggcg
     3601 atacccagcg ggcgtttcag ccagccaggc gcaagctcag tcaggaacag ggcgataccg
     3661 aaactcaccg ggacggcgat cagcagcgcg ataaacgaag tcaccaacgt accgtagatc
     3721 ggcaccagcg ccccgtagat atcgttcggt gcatcccact ctttggtcca taggaaagcc
     3781 agaccaaatt tctgaatgct cggccaggag gagatgatca gagagacaat aatgccaccc
     3841 aacatcaata gcacaatcag cgccgccagt tttaccagca cgctgaaaat tatgtcgccc
     3901 tttttacccg gtgggttaaa agcgggcttg gttgcagcca taagttactc ttcagttaaa
     3961 cgcgtttaca aagtcatttt gtaatgccgg atgcggcgta aaacaccgta cccggcctgg
     4021 agttttatta gtacagcggc ttaccgctac tgtctttaat attggtcttc cacgcagcgc
     4081 gaacctgttc aactacacta tccggcaggc tggcgtaatc caggtcgttc gcctgtttag
     4141 ccccggtttt gtacgcccag tcgaagaatt tcagcacctc tgtgccttgt tctggtttct
     4201 tctgatcttt gtggatcaga atgaacgtgg tagaggtaat aggccatgca tcttcgcctt
     4261 tctggttggt cagatcctga gcgaaggttt tgctccagtc tgcacctttt gctgcattag
     4321 cgaagttttc ttcggtcgga ctaaccggtt taccatcagc ggagatcagt ttggtgtacg
     4381 ccaggttgtt ctgcttcgcg taagcatatt caacataacc aattgcaccc ggcagacgct
     4441 gaacgaacgc ggcgataccg tcgttacctt taccgcccag accgatcggc cattttacgg
     4501 tagagccagt accaacgttg tttttccact cttcgttcac tttcgccagg tagctggtga
     4561 agacgaagga agtcccggag ccatctgcgc ggcgtactac agcaatgttt tgtgaaggca
     4621 gtttcagacc cggattcagt ttggcgatgg cttcatcatc ccacttcttg attttgccca
     4681 ggtagatgtc gccgagggtt ttaccatcca gcaccagttc gccagacttc agccctggaa
     4741 tgttaaccgc cagcaccacg ccgccaatca cggtcgggaa ctggaacaga ccttcctgag
     4801 ccagtttttc gtcagacagc ggcgcgtcag aggcaccaaa atcaacggta ttagcgataa
     4861 tctgttttac gccaccggaa gaaccgatac cctggtagtt aactttatta ccggtttctt
     4921 tctggtaagt gtcagcccat ttggcataca ccggcgcagg gaaggttgca cctgcacctg
     4981 tcaggcttgc ttctgcaaac acagagaaag cactcatcga taaggtcgcg gcgacaacag
     5041 ttgcgacggt ggtacgcata actttcataa tgtctcctgg gaggattcat aaagcattgt
     5101 ttgttggcta cgagaagcaa aataggacaa acaggtgaca gttatatata aggaatatga
     5161 catttttatg acagaaagga attaccaaat aacttactga ttttaaatgt gaagatgaaa
     5221 atcccacatg ccatcttttt gtcatgatgc atttaaaagt gataaagaaa tgggtgagat
     5281 gacaacgatg tgataaacaa tcgttgtcag acctattagc gcacaatctc aacagttatc
     5341 gtcgctgttg cagtaaattg ccccggggct gggcgagctc cagtaaggct cgcaggagca
     5401 gctgaaaaag ttacactacc tgatttttca ccatacactg ttgatttacc catgtctgtt
     5461 ggcaactcac caccgttcac tttgatttcc ttattatttt tgtcgtaaat ttttatcccc
     5521 acgttagagt ttgaagttac gaccattgaa ttgtccgtat tgggttcggt agcataaaaa
     5581 gacgta
//
LOCUS       DADLSE010000207         5582 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLSE010000207 DADLSE010000000
VERSION     DADLSE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.208"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..502)
                     /locus_tag="JN692_003851"
     CDS             complement(<1..502)
                     /locus_tag="JN692_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266010.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSK"
     gene            complement(510..2858)
                     /locus_tag="JN692_003852"
     CDS             complement(510..2858)
                     /locus_tag="JN692_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7266011.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEVIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(3036..3947)
                     /gene="gsiD"
                     /locus_tag="JN692_003853"
     CDS             complement(3036..3947)
                     /gene="gsiD"
                     /locus_tag="JN692_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7266012.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(3950..4870)
                     /gene="gsiC"
                     /locus_tag="JN692_003854"
     CDS             complement(3950..4870)
                     /gene="gsiC"
                     /locus_tag="JN692_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7266013.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(4888..>5582)
                     /locus_tag="JN692_003855"
     CDS             complement(4888..>5582)
                     /locus_tag="JN692_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="with GsiACD is involved in the transport of
                     glutathione into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7266014.1"
                     /translation="PKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKP
                     WPYDPVKARELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMD
                     AGQRAAEVEGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNK
                     QVDDFLAQALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMP
                     DTGFSFEDADLQ"
BASE COUNT         1582 a         1443 c         1183 g         1374 t
ORIGIN      
        1 attttgacag taaagaaaaa cgctccagac caagcaaaaa tatcagacta cgattgtgag
       61 ttaccggata gaggtaatag acgaaggttt taccgtcaaa agagataaac cctgtatttt
      121 tgacatgtcg atacttcaca acctgcataa tatctgcaca ataatttttc tcgaactcct
      181 ttacctgaat acattcgtca ctggaaatca gataaggacc gacatcgtac aaataagagg
      241 gggaaaccga gtcggcgtaa gctgtgcgca aaccaaccaa cagctgttcc tccgtgggat
      301 ttagcgtgtt gataagatca attgccacgc tacgccgata ttcagcaata tttgccaccg
      361 caaaagcagt atcaataacg gactgtttca ggctatacac ccgttccttt accatttgag
      421 tgtagatccc gcaggcaact gctgaaatca tgataaaaat cagcagactg actgtaagta
      481 cgaacttatt gattctggac atacgtaaac tactctttta ctaattttcc acttttatcc
      541 caggcggaga atggcatggg tttctggtac agatacccct gagcctgaac acatcctaat
      601 ttaattaatt cgcgcgcaac gtcttcgctc tctacgcctt ccgcaatcac cgggatccgg
      661 ttatagcgtg ccagatcgaa aataatgcgc acaatttttc ccgcagtacc gaaaatatcg
      721 ccggtaaatt gcgagtccac ctttatgctg tctggattaa tttcataaaa acgtttcagg
      781 tttgacaaac cggtgccaaa gtcatcaatc gatatccgta ccccttgttt acgcagttgt
      841 tcaatattga gataaaaatg ttcattaatt cgctcgatca cggtttcagt gatctcaaca
      901 cagaggctgt tcgccttcag gccattttct ttcatgatgg tggtaaaacg ctcatgaaag
      961 tgcggttcgt ttaactgcca gggcgaaaca ttaatatgca gtaagaaatc atccttcagc
     1021 gacgcacgtt gactaaaatg tctgaattca cccattgccg ttttaataat ctgataaccc
     1081 agctcattga taaacccgat atcttcagca atggtaatga acttcagcgg tgaaatgatc
     1141 ccccgctccg cagaaaccca acggcacaac gcctccgcgc tcagaatggc gcgggtgtta
     1201 atatcaacga tcggttgata gacgaggaat agttcgccaa tttccattgc atggcgaata
     1261 ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt tcgcgtcatc acagaaaata
     1321 aactcggaga acatatggtc acggatactg gaaatcgcca gtttcagttt ttctgcgtaa
     1381 ctccaggcgt tttccccctg caaaggttca caaatggcag acttatggat gcgcagattg
     1441 cggtagccaa agctgctgtc aaaatcgttt accatcgcta ttttacgggt aaatgtctgt
     1501 acattttccc gacacagaag taaatagaga tcggcattga atgtcacgat atcgcagcat
     1561 tgcgcatatt tctcggcaat tcgctgacac aatttcctga cgattttatt gcgttcatca
     1621 acaccaaaca ccgcttccac ggcttccaga ccacacagtt tgatgatccc cacagccaga
     1681 tactgattat tgttggactc aataatcgcc atatttttaa taaaaccgct ttcctgatcg
     1741 taagagatca gtgagagcaa tttatttgac cgttcaattt cggcatcttt catttccgcg
     1801 atcgaatttt tgatgctgat aatctctttc gcatcgcctt cgcgaatttt cagagcctcg
     1861 atctgatccg ttgagtcggg caaaaacgcc agcgtattaa ccagctcggt catttcagaa
     1921 aattgccgac ggaacacacg agtggtcatg aaatatacca ccagcaacac gataataatg
     1981 aacgcaaacg gtagataaaa aagtttatct gatgcgttca ataaattacc gccaggataa
     2041 taaaaataga tttcgaacaa tgcattattt ggcactgctg atgtctttgc aatatactcc
     2101 gtatcgacag aatcaataat attataatta ccttcgctat tataaataat cagtttttca
     2161 cgagcgagtt gattcttggc aatgacctca tgaccttgtt tcaaaaatag aacttcatca
     2221 ttagcgctga tttgatcaag atactcgttc agataccgcc agtttaaatc gacatgaatt
     2281 ttatctaccg ctaacaaatc gtgatctttg ccgttgatac gtagcgtaaa agagatacat
     2341 tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt agagtcatct
     2401 gaagccaccg accacggtcg tttcgatggg ttaaaatcgg caaaaataac ccgccctgac
     2461 tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt atcattcgcc
     2521 ctccgcgaaa agagataata tttattatca tcaagtacca gtcccaccga gtctaaaaat
     2581 ggcatcattt ccattttcag aatcacggcg cgttgaatct cctctgtatt ttttaataaa
     2641 tacagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa atagccaacg
     2701 tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc cgataccagt
     2761 tgatagttaa ttatgaaaaa aagaccaata aatgacagtg ctgccaataa aaataaaatt
     2821 atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa
     2881 ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt aaccaaatta
     2941 cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata atttcctgtg
     3001 aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt cggatccagc
     3061 gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa aatagccagg
     3121 gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc attgagcatt
     3181 gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag gctggcggca
     3241 gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagaaga gacggtccca
     3301 ggcaggatat gacgcaataa aatggtcata tcgctggcac caatactgcg tgctgactca
     3361 ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccaggcgggc aaacgcgggg
     3421 atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa caccgcaaca
     3481 acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat gcgcatgatc
     3541 agccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt ccctatcgcc
     3601 acaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc aaccaggaca
     3661 cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa agaaggtccg
     3721 ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat ccagcgtgca
     3781 aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc catatgctga
     3841 cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgta cctggtcagg tttgaccagt
     3901 ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct tacttgtacc
     3961 tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata agaataaatt
     4021 ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt tctacagagt
     4081 caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg atagaaccac
     4141 cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc gcgttgcgta
     4201 gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg gtacgcatat
     4261 aatcttcgct taaaacgtcg acaaatgacg cacgggtaaa gcgcgccatc acggcggcca
     4321 ctgccgcgcc gagcgtcagg gagggtaaaa tgtagtgctg ccaactgtct gctcccacag
     4381 taggcagcca gcccagttca acggagaata cctgaattaa aagcatcccc agcgcaaatg
     4441 ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc caacgattac
     4501 gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag accatactgg
     4561 ttatggtcag ccacagcgtt ggcataaagc ggctggcaat ctcatcggca accggacgac
     4621 gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag tgccagaact
     4681 ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata acctgcgcat
     4741 cagcttcggg cccggcaatc aatcgcgccg gatcgccggg cagcatgtgg acaaataaaa
     4801 acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaatcgt ttgataacgt
     4861 aattaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa agctgaagcc
     4921 ggtgtctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca ccagtttttc
     4981 taccaccagc gggatccacg gcgattcttg ccagatgata tcctgcgccg ccttatataa
     5041 gcgggtcttt tccgccggat cattcgtttt cagtgcctga gccaggaagt catccacctg
     5101 tttattgctg taaaacgcgg tattaaacag cgtcggtggc cagttttgcg aggcaaacag
     5161 cggcgatagt gcccagtcgg cttcgccggt tgaagccgac cagccagtgt agaacatccg
     5221 cacgccgctc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc cggcatccat
     5281 cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa attgcagcac
     5341 tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac cgttgggata
     5401 tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg gtttataact
     5461 ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggcgttgcat agcccgcaaa
     5521 ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac ggaccttcgg
     5581 gt
//
LOCUS       DADLSE010000208         5580 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLSE010000208 DADLSE010000000
VERSION     DADLSE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.209"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1160
                     /gene="espX1"
                     /locus_tag="JN692_003856"
                     /pseudo
     CDS             1..1160
                     /gene="espX1"
                     /locus_tag="JN692_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            1482..2015
                     /locus_tag="JN692_003857"
     CDS             1482..2015
                     /locus_tag="JN692_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266015.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSEMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            2065..2748
                     /locus_tag="JN692_003858"
     CDS             2065..2748
                     /locus_tag="JN692_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7266016.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAEKNKIKLALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNA
                     ISINNETANWITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGN
                     NIHSKIAAR"
     gene            2761..5212
                     /locus_tag="JN692_003859"
                     /pseudo
     CDS             2761..5212
                     /locus_tag="JN692_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            5225..>5580
                     /locus_tag="JN692_003860"
     CDS             5225..>5580
                     /locus_tag="JN692_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7266017.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLLETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDDKKIEL
                     PGKSSYNLKEMLSAIN"
BASE COUNT         1924 a         1065 c         1013 g         1578 t
ORIGIN      
        1 tcatagaaca tacagttcaa ttttcttttt ttaaacacaa aggcatcaca ttacttagat
       61 tggatgtttt tgatgacaga acaagcgaat gtctttctga agagattaag atatatcaag
      121 aatgccatga aaaatttatt aaatttttaa aggcaaactt caaccaggaa atatacccag
      181 agctatacac tcctgaaata ttttatgagg cctgcagaaa tctccagtca ttttatgatc
      241 atcaggagtc gtcacaaaac gctaaataca gtgcaatagt caagaaaaaa agttatttta
      301 ataaggaaat tagaaatcta ataaaaaaga atatttatcc tgagctatac aatgagcaat
      361 gcaataaaat ccccgcctca tcgacagatg ataaccagaa aataacatgg cagaatttta
      421 aaactagcaa tgcggcatat tcacaactat gtgaaaaatt atctttatta aaaagctcgc
      481 ccagtcggtt aatagagaaa tcagcttact gctcaaatga aaacatgatc accgataaat
      541 ttaatgttgt cttttcttac tgcggagata atgtaaatga atttatactt ttattaccct
      601 ataataaatc tctggagatg catgaactta acgagcaaaa catccagtat ttgacttccc
      661 ttaatattaa tatacataaa atgttgctat caaatataac aatagaaaag agcaaccttt
      721 catatgggta ttattttggt tgtgtattat ccaatatatc atgttttgaa agcgacttat
      781 caaacaccat cttttcaaat ggagaaataa acaatccctt cattaaaaaa agcaacatat
      841 ttggaacatc gttcactaat accatgataa aaaatttgcg ctgtgaagat attatgcctg
      901 gccgatggac cactcaactt gtcaataagc acttaggtta cagatacaca ggagtattca
      961 aaaccctggc aagtattgat gacaaaccgt cccgtttcga aattttgata ccattggtac
     1021 aaactttagt tcgcgacaat gtaaagctaa ataatgatgt gtataaagaa ctgaaaaagt
     1081 ttatgcacga ttacgacaaa acatccccag aaatgagaaa ataccttcag agcatcaacg
     1141 agtctatgtt ccttataaaa aaatatctca tcaagattaa aacaccccaa aaaataagta
     1201 atttcagaat gaaactacct gtgataatta ttaattaact gcagagaaat aaaaaataat
     1261 ctgaagcatg ctctgaaaca tcctactctg tcactctaat gatggcacct tgctcttctt
     1321 ataaagaagg gaataattaa gaatattaaa ttttcaaact ttcatcaact tcacatcaag
     1381 aaattaaaaa atttaaacat taatctaaat aataacaacc tgatcacata atcgggtttg
     1441 aaaacgagtg accttccata ccagttaatt aaggatgatt tatgaaaaag tatattattc
     1501 ccttcgttgc gacaacgttg atgtttagcg caacaacctt cgccaaggat gtcgatcaag
     1561 gaacactaac cattagagga gaagttgttg atacaacgtg ttactttgtt aatggtgatt
     1621 cgacaacaga tatcgttgtc gaagatgttg taaccagtga aatgaatcaa cttatcaata
     1681 atgaaatcta tactttaaaa accggttcaa cctctcatga cttagaaatt cagtgtgacg
     1741 gaaatacagc cccaagtatc gaagtactag catcagagtt tgatgctaat aactttacgc
     1801 tgaataaagg aaccgctgag aacattggct ttgccatttt cctcgatgat gggactttga
     1861 ccagaatgag accagggtta aaaacaccgc tgaaagataa tggctctggt caatattttc
     1921 ttaatttgaa tgcacaatat gcccgcacaa gtggcaatcc agttacaaaa ggctccgtag
     1981 agtcaaccgt aacgattaaa atcagtgcag attaacgttt tttgccggta gctctgctac
     2041 cggtattatt tacggagtga ttacgtgaga ataataacag cattactgat gtcatttttt
     2101 attttacctg ttcatgccgg tgtggtcatc tatggtacgc gcattattta tcctgaaaaa
     2161 aacagagaag ttctcgtaca gttaatgaat caaagtaaaa atgcctcttt aattcaggca
     2221 tggatcgatg atgggaacac cactatcgcg ccagaaaaaa ttcaggtccc atttattctc
     2281 acaccgccag taagtcgtgt agcaggtggt tctggccaac aacttaaaat aagaaaaatg
     2341 ccaaacaact tgcctcacaa taaagaaagt ttattttatc taaatgttct ggatatccct
     2401 ccaaacaacc cacaaaatgc agaaaaaaac aaaattaaac ttgcattgca aaaccgtata
     2461 aagctacttt ggcgtccaag cggaatagct ccggtagata aaaaaagcct ttctcaatta
     2521 aatatcaaaa agaaaaataa cgccatctca ataaacaacg aaacagccaa ctggataacg
     2581 gttacaacta tcaaagcaca aaacgtgaaa gttaataatg aaagtatctt actgcctcca
     2641 ttttcaaata acgacattac attaaaaaat aaccacgcat cagaatatga actaacggta
     2701 gttgatgatt acgggaataa catccatagt aaaatcgccg cgagataaca ggaaaaagtc
     2761 atgttaagga cgacacgatg ggtcgctgct attatttttt tgtattcttt tccgggttat
     2821 gctgaagaaa attttgatac gcactttatg agtggcggaa tgagaggaga aaaagtttcc
     2881 gaatatcgtt tcgataataa acaaccgctt ccaggaaact acgaacttga tttttatgta
     2941 aataaccagt ggcgtggaaa acaagatatc acgatcccag agtcaccagt caaaccatgt
     3001 ctgcctaagg tactactaac caaactgggg gtaaagaccg gtaacctcaa tacagaagat
     3061 aattgtattt tactggatga agcggttcat ggtggacaat atcagtggga tatcagtgaa
     3121 caccgactaa atttaacagt accacaggcg tatatcaatg aacttgaaag aggctacgtt
     3181 cccccagaaa gttgggatcg cggcattgat gctttttata cctcctataa tttaagtcag
     3241 tatcgctctt atgacagcaa taataacagt catacagcca gttatggccg gtttaacagc
     3301 ggattaaatt tatttagttg gcagttacac tctgatgcca gttatagcaa acctgatgat
     3361 atgaaaggca aatggcaaag taatactctt tacctggaac gtggctggtc gcagatatta
     3421 agcacagttc agataggaga aaattacaca tcctcactca tattcgactc tctgcgattt
     3481 agcggaatac gcttatttcg cgatatgcaa atgctacctg attctatgca aagctttaca
     3541 cctttagtac aaggtgttgc gcaaagtaac gccttgataa cggtttcgca aaatggctat
     3601 accatttacc aaaaagaggt tcccccagga ccatttacta tcgcagattt gcaactttcg
     3661 ggcagcggct ctgatctcga tgtcagcatt aaggaagcag atggtagtgt tcgttccttt
     3721 ttggtgccct actcttccgt gcccaatatg ctgcaacctg gtgtttcaaa tttcgatttt
     3781 atcgccggac gtagtcagat atatggtgtc aagaatcagg aggactttct cgaggcaaat
     3841 tatatatatg gcctcaacaa tcttttgaca ctttatggcg gtacgatttt atccgataac
     3901 tataatgcta taactctggg aaatggttgg aatacaccgc tgggagcaat ctcttttgat
     3961 gctacccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
     4021 gtcgcctata ataaatatct attgcaaacg gccactcact ttagcgtggc cgcctagcgt
     4081 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaataa taaaataaat
     4141 catcagagtg actatgatga tttttatgat attggcagaa aaaaatagcc tttctgccaa
     4201 tataatgcaa cctttatcca ataatctggg aaatgtatca ttaagtgcac tttggcggaa
     4261 ttactggggg cgaagtggaa atgctaaaga ttaccaattc agttactcca ataactggca
     4321 acacattagt tatactttct ctgccagcca atcctatgat gaaaacgata aagaagagga
     4381 gcgttttaat ctgtttatct ccattccttt ctactggggt gatgatattg ccaaaacacg
     4441 tcaccaaatt aacttatcga attctacctc attttccaaa gatggctatt cctccaacaa
     4501 tactggaatt actggcatag ccggtgaaca tgatcagtta aattatggta tatatgttaa
     4561 tcagcaacaa caaaataatg atacctcgct tggtacgaat ttaagctgga gaactccaat
     4621 cgccacaata gatggcagct atagccattc taaaaacgcc tggcaaagtg gtggaagtat
     4681 tagtagtgga ttagttgtct ggcccggtgg tattaatatc actaaccagc tatccgatac
     4741 atttgcaatt ctggatgcgc ctgggttaga aggcgcgcat attaatggac aaaaatacaa
     4801 ccgaacaaac agcaaaggcc aggttgttta cgacctgatg atacctcatc gtgaaaacca
     4861 tctggtactt gatatagcaa acagtgaaag tgaaacagaa ttgcagggca atcgtcaaat
     4921 tattgcgcct taccgtggag cagtttctta tgtgcagttt acaactgacc aacgtaagcc
     4981 ctggtatata caggcactgc gtcccgatgg ttcgccatta acctttggct atgacgtact
     5041 ggatctccag gaaaacaata ttggagtcgt tggccagggt agtcgccttt ttattcgcgt
     5101 agatgaaatt cccactggca taaaagttgc tctcaatgat gaacagaatt tattctgtac
     5161 tattactttt caacacgtta tcgatgaaaa caaaacatat atatgccagt aaaaggagtt
     5221 tactatgaag tggcttttat taatcacctt gagtctatat tcttttattg tgcaaagtgc
     5281 tccctgtgga ttgaccaatg tcggggaaca acgtggaaca tatatactca agccactgag
     5341 tatgaaaggt aatctcaccg ccgaaaaaat tgaaaacacc ggaaatatca ttgagtgtgc
     5401 agacttgcta gagacgacta acgccattaa agttcatttt ttaacacaga acgctttaag
     5461 gtttaaagtc ggaagtcgtc attatattat taatttcccc tttgaatctg acgataaaaa
     5521 aatagagctt ccagggaagt catcttataa tttaaaagag atgctatctg cgataaattt
//
LOCUS       DADLSE010000209         5541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLSE010000209 DADLSE010000000
VERSION     DADLSE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.210"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(57..476)
                     /locus_tag="JN692_003861"
     CDS             complement(57..476)
                     /locus_tag="JN692_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266018.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILTSSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            526..1704
                     /gene="ltrA"
                     /locus_tag="JN692_003862"
     CDS             526..1704
                     /gene="ltrA"
                     /locus_tag="JN692_003862"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7266019.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1787..1966
                     /locus_tag="JN692_003863"
                     /pseudo
     CDS             1787..1966
                     /locus_tag="JN692_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1996..2478)
                     /locus_tag="JN692_003864"
                     /pseudo
     CDS             complement(1996..2478)
                     /locus_tag="JN692_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2734..3714)
                     /locus_tag="JN692_003865"
     CDS             complement(2734..3714)
                     /locus_tag="JN692_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7266020.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3714..4913)
                     /locus_tag="JN692_003866"
     CDS             complement(3714..4913)
                     /locus_tag="JN692_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7266021.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5235..>5541)
                     /locus_tag="JN692_003867"
     CDS             complement(5235..>5541)
                     /locus_tag="JN692_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7266022.1"
                     /translation="TGGVLAYTFGPRNDETCRELLALLTPFCIGMVTSDDWGSYAREV
                     PKEKHLTGKIFTQRIERNNRTLRTRIKRLARKTICFSRSVEIHEKVIGSFIEKHMFY"
BASE COUNT         1533 a         1261 c         1309 g         1438 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtggaggtgg gccctccggt cagggtttac
       61 aggagcactg accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt ggggttcggg aagctgaacg aaagtaaact
      181 gtccgatgaa gcatcgaaat cttagatgat gtcaaaatgg ggccgtacag ccgtcccctg
      241 ataagcgcag aagacacctg cttactgact gcgcggcatc cggtgtgaag gcagcaggat
      301 cccgggccag accatctcag ggaacgtgag aacctgtttc cggcacgtat cgggaaacag
      361 gggcggaccc attcgtagta gtgatgaaag atctgtaatg ggtctggagc gaagggatgg
      421 ggatcacatt cattgcagaa gacacaaccc aaaaggggat gacgtaactg atgcaacgaa
      481 aatcatttga aataccaaaa gcactggtct gggcatcata tctggatgtg cgtcgcaaca
      541 agggagcccc ccggatgcga cgggcagact ttgaaaatgt tcgaccagca acgtgatggc
      601 aatctataca agatctggaa ccggctatgt tcgggaacat ggtttccgcc accagtgctg
      661 gaaaaacgga tcccgaagtc gaatggcaag gaacgcattc tggggatccc aacagtatcc
      721 gatcgaattg ctcagggagc gataaaactt ttcatggaag aaaagcttga cccgattttc
      781 catgctgatt catatggtta ccgaccaggc aaatcagctc atgacgctct gaaacaatgt
      841 gccatccggt gctggcgtta tagctggata ctggaagtgg acatcagtgc gtttttcgac
      901 catgtgagac atgatctggt gctcaaagct ctggaacacc acgggatgcc taaatgggtc
      961 atcctgtact gtcgacgctg gatggaagcg ccaatgcaga gttgtgaaaa tggagaatta
     1021 ataacccgaa cacgaggcac tccgcagggc ggggtcatta gtccgctact tgctaacttg
     1081 ttccttcact atgcatttga tttgtggatg gaaagagaat atcggggggt accgtttgag
     1141 aggtacgctg acgatattgt agtacattgt tcacgaatga gtgatgcaac aaggctaaag
     1201 aacaggttat cggagcgctt ctcggaagtc gggctggtcc tgaatgcagg gaaaacgaat
     1261 atcgcctaca ttgacacgtt taaaaggaga aacgtcgcaa cgagtttcac ctttctcgga
     1321 tatgacttca aagtgcgtac gctgaagaat ttcaaaggcg aacggtaccg aaaatgcatg
     1381 ccaggtgcgt caaatgcagc aatgcgcaaa ataacagaaa caatcaagaa gtggcgtata
     1441 catcgctcaa cagctgagag tttgctggat tttgcgaggc gctacaatgc gatagtgaga
     1501 ggctggatcg agtactacgg aaagttctgg tccagaaatt tcaactatcg actgtggagt
     1561 gcaatgcagt cacgtctgct caagtggatg cagtctaaat acagactttc gaaccggaag
     1621 gctcagcgaa agctgacgct ggtaaggaag gagtatccga agctatttgt tcactggtat
     1681 ttactacgtg catcaaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1741 tccgtgagca gccggggagt gaaattctct ccggctgact cgactcgcgg gttccgacag
     1801 cgatgacgaa catgcggcgg tgttgtactc gctgatcggc acatgccgtc tgaacaatgt
     1861 ggaaccagaa aaatggctgc gttacgtcat tgagcatatc caggactggc cggcaaaccg
     1921 ggtacgtgat ctgttgccct ggaaagttga tctgagctct cagtaaatat caatacggtt
     1981 ctgacgagcc gcttactttt ggcgtaattc tgctgcgcct ttactgaaca caatgccgta
     2041 ttcagcaaga agtccacgaa tgtgatttat cagttgtgtc ctttccattg aaaccaaccg
     2101 acgcatattc aaaaaagtct ggacgtcctg ttgctccaga gtttttattg gtacgggtcg
     2161 tatagttcca tgttggcagg cctcagctat tgcctgtgcg tcgttataat catttttctg
     2221 gcaccgctga taagctttaa catgttgtgc cggaagcaat attacatcat ggtcgagttt
     2281 cctgatttcc cgggcccagt aatgagatgc cccgccagcc tccatcgcaa cagtcatttt
     2341 aggctgttgc accagccagt taagcagttg attgcgagaa agtttggtct ttcctgcggg
     2401 tttgccatga gcattgatac tgaaaagata aaagttcgtt ttagccagat caatgccggt
     2461 gattttctgt tcagacatgg tatcccgctt acttttgtgg ttaaagttta tccatcatgg
     2521 cgcaattgaa ggctgccgtg aaagaggggg aagactatct catcggttac tgttaaacgg
     2581 ctaagagaaa ctatcttttt caagcaaaga agatacggtg aaacaggcga tttcaccgtg
     2641 aaatactgtt caaaaaaaac gctaataaaa gcaaaatttc ttttaaaaca gccaattaaa
     2701 aactggtgag ttgctggcga aaaacttgaa ttttcacagc gaattcagat tatcttttag
     2761 tacaagagca atcatacgat ctaacttatc ctgaacttcc aacggtaatc tgccgaattc
     2821 atatgagaac gtacgtccct ttactcgttt tcgagcaaat ttatctttac tatcaaattt
     2881 ccataacaaa gttacaaccg ctttatcctt tactcctgaa tcaagcaatg aagacatttc
     2941 cttggttata agccgcataa tactgttttt aacctcgtct tcagaaagat tgtcatcagc
     3001 agtaataagc tcaatactcg gcgagatatt cttcacaact tcatcaatag atatatcacc
     3061 cagcctcagt cgttcactta attcagccag ttgcctgtaa tcggcgtatg tcagctcaga
     3121 ctgaacagga aaaagagaca caaaatcttt aggtacggac gctgcttgga gggcacgagt
     3181 caccttagct gccgacaatc cctctctttc cgcaatttgc ttctggttca taccggcctc
     3241 tttgagacgt actaaacgca acccaatttc acgaatatta tgttcaagtg acgtctgcaa
     3301 atctttcgcc agatgctgag cttcagaaac agtaagctca tcatccgtta caagtacacg
     3361 taatccaacc ttgcatagca aagctgctgc ccgacgacgg gaaccatcca aaatctcaat
     3421 aagatcgcca gtccgaatcc caatacatgg atagaattgt tgtaaacgta tagttctggt
     3481 gatgtctttt agtgagtccg gcgtcagggc tgtctgttcg cggccattca cctcttcaac
     3541 cacaaaagtt tgagtgtcca ctttgtcatg atcaaccttg atctcagtaa attttgcctt
     3601 tcggccagta ttgagggtaa atacatgaac tggagccgat atttcttcag tattattcag
     3661 gatatttgta ttcagcgtac ggccaatagt cggtcggtgc tttctgtttt ccatcagtag
     3721 ttggccctta taaattcaat acgatcaaaa actgctttag cgaaatcttc tgctgccata
     3781 cgtgcatttt tcaaagcttc accactgcca acataagtaa caggattcgc agatatcacg
     3841 gtatcaaacg actcgccgct tctctcaaaa ccatcaagac gtggcattga aacatcaagc
     3901 atgtcaccac cgaaaatctc ttttgtcagg ctatggcagt atttatgatc tgacttattc
     3961 gccagtttcg acatgaagcc aatatttgcc tgcaaacggc atgaacatcc atcctgttca
     4021 ataatctgta caagttcagg taaacgagcc aaatatttaa gcgtagaatg gaaatcaact
     4081 tgtgctggcg gcaccggtgt aaacatgata tctgcggcag caatggcatt tttcaaaaat
     4141 gcgtccagat gcgggcctgt atcaataagg atgaagtcga aatcatgctt cagcttatca
     4201 ataatattct cacgcaatat cgcgtgtttg ttttgtccta atagatgctc ctcgcacaga
     4261 gtatcccagt ttgatgcaat aaaggcgtcg tcaatagaag ccggcataac ataaacgcca
     4321 gggataactg acggaacgat aaaatcttcc agtaattcct cacgactcac gttctgcaac
     4381 atagcctgcg gtgcggttgt atcaaccagc cccaccgcat gcaagtagtt cagaaacata
     4441 gtggctgatg actggggatc taaatcaagg agcagaatac gtaaatcttc tgacagcagg
     4501 tgaggatgag cacgcagggc gtgagctacg gaaacactac tcacggtttt agacacaccg
     4561 ccttttaaac taccgatgaa tatggaatat gcctcactat aacgatcgcg atatttagga
     4621 atgccacgat gtgcatagat atcgataatg ttctggatgg ttaatgcata ttttgtcgcg
     4681 gttcctgctg ggcgtttttc aaactgatag ccctgagcct ccatctcacc gactgcacca
     4741 tccacgcttg cgcgcgttaa ttttggaagc ttagctacag cagactttga atagacttga
     4801 taaaaaacat cagcctgaaa ctcctgcttt tgctcctgaa tctgttttgt tagggcgagc
     4861 aacattttat ctgcacgctg cgcaacctta cttaactgct caaaactagt cattccccct
     4921 ccgtgccatg taggatttta acttattaca aaaaaaactg cattagcagg gtaatgcatg
     4981 caagtacata ttgtacatgc ttatattaaa aatgcgcgca ttgctgattt attaatcatc
     5041 ggaatgaata ttactgtaag atagtatgcc tttcggggag aaagacgctg atttcccccg
     5101 aataatccag ttcaccacca cactccggac aacagctttc ttcaggctcc agatggtgta
     5161 tttcgcggga aacatgttcc ggtaacggac ggcgatgacg tgactgtcgc aacaggcggg
     5221 ggtagtgctt ccaatcagta gaacatgtgc ttttcaatga aggagccgat gactttttcg
     5281 tggatttcca cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcgggtg
     5341 cggagggtcc ggttgttacg ttcaatacgt tgcgtgaaga ttttgccggt caggtgtttc
     5401 tccttcggca cttctctggc gtagctgccc cagtcatcgc tggtcaccat gccgatacag
     5461 aacggagtca acagggctaa cagttcccga caggtctcat cattccgtgg cccgaaagtg
     5521 taagccaaca caccgccagt t
//
LOCUS       DADLSE010000210         5445 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLSE010000210 DADLSE010000000
VERSION     DADLSE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.211"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..185
                     /locus_tag="JN692_003868"
     CDS             <1..185
                     /locus_tag="JN692_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7266023.1"
                     /translation="GMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNI
                     NAVAMKITADIMQHAD"
     gene            complement(339..1988)
                     /locus_tag="JN692_003869"
     CDS             complement(339..1988)
                     /locus_tag="JN692_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7266024.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2139..3488)
                     /locus_tag="JN692_003870"
     CDS             complement(2139..3488)
                     /locus_tag="JN692_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7266025.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTVFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFVTFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN692_003871"
     CDS             complement(4194..4658)
                     /gene="soxR"
                     /locus_tag="JN692_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7266026.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4744..5067
                     /gene="soxS"
                     /locus_tag="JN692_003872"
     CDS             4744..5067
                     /gene="soxS"
                     /locus_tag="JN692_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7266027.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5070..>5445)
                     /locus_tag="JN692_003873"
     CDS             complement(5070..>5445)
                     /locus_tag="JN692_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7266028.1"
                     /translation="MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSF
                     VETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKA
                     MPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1460 a         1525 c         1309 g         1151 t
ORIGIN      
        1 agggaatggg catttccatt ctccgtgggg aggaaaatat ccccattgat agtgataccc
       61 tggtaacact acagaaattc tttgaagaag attgtacctc tcatactggc atgtcacaaa
      121 ctgaggataa tattaatgca gtcgctatga agattactgc agatattatg caacacgcag
      181 attgaggtta agtcgaagaa cagccaatga attgacttta caagagagat aacttatttg
      241 ttttagaatc atgaatgttc gcccgagcta acctgttaaa ggtgcaaaag attgcacctt
      301 tttcagatta cctcgggcct ggttacgtcg ctttcggctt actgattttc ctcaattagc
      361 aacgcttcaa gcaaatcgag atcgtgcagt aatttttgca gcgtttcgtt gctgatctcc
      421 cgcgtggcgc gcaggtggta cagttcagca cgttcagaac gcaatgccgc cagacggaag
      481 cgacgctcaa ggttctcttc ctgcacggaa ctttcaacgt cgttacgtcc atcggcgcga
      541 cgacgcaggt taccaatgac gcgagaactg acctccgtaa gcaactggtt atcgatattc
      601 tcttcggtat ccgccgccag acgctcctcc attttctgga tcgccacaat cgccacttct
      661 gccgttgccg ctcgcgcaat acgttcttct ttcaattgtt gcgaatggtc cgcgacttca
      721 atgtgttgta gcagaatagg caacatcacc acgccgacaa acagcgagaa gagaatgacg
      781 ccagccgcta ggaacaccag ctcatagcgc gccgggaaga cgttaccatc cggcaagagc
      841 agcgggattg agagcacacc ggccagagtg atcgccccac gcaccccagc gaaagacgcg
      901 atcaggattt ctcgtgtggt ccacgaacca aactccatcg gcttcttctt caggaagcgg
      961 ttgctgaact ttttcatcgt ccacagccag ccgaaacgga ccagcatcag cgccgcatat
     1021 atcagaataa tatcggtaaa cagcatccag atttcgacgt tagggtcgat ttctgccgcc
     1081 atcagcgacg tctccagaat acccggcagt tgcagaccta acagcaggaa caccatgccg
     1141 ttaaagacaa attccagcat cgcccaggtg ctgtttgcgc gcaggcgcat tgccagtggc
     1201 gcacggcgca tcacaccgga gcgggtgatg gtcatcccgg cggcaaccgc agcgaggatg
     1261 cccgaaacgc caatatgttc ggcaatcaga taagaagcga atggcagcaa gaacagcaga
     1321 acgatctgcg tcgcgggttc atcaccgccc cagcggctga ggaatcgcag cgagcgacca
     1381 tacagccagc tcaccacaaa accggcaaga ataccgccaa tggctacttt cataaattcg
     1441 accgtcgcac cgccgacagt gaagatcatc gtccccatcg ccactgccac ggcaaacttc
     1501 aacgacacca ggccggaagc gtcattcatc aacgcttcgc cctgcaaaat gcccatgatt
     1561 tttttcggga tgcgcccttc gccaacaatc ccggagagcg ccacagcatc ggtcggagaa
     1621 agtaccgccg ccagcgcaaa ggcggggatc agcggaatgc ccggcaccac ccagtaaata
     1681 aggaagccaa tgccgaccac ggtgaccacc accagcgcca gcgcgaggcc gaaaatctct
     1741 cgaccatgtt cgagaaattc acgggtcggc gttttccagc catcagcgaa cagcaacggc
     1801 gggataaaca agactaaaaa gagttcagga tcaaactcca catgcaaacc aaacgtcggc
     1861 cacgccagta gcgcaccgat ggcgatttgc ataagcggaa gcgggatctg aaagggcatg
     1921 acacgagtga ccaccccgga cagcgagacc acgagggtca ttatcagtat ggtgaagaag
     1981 atttccatgc gttccctgtt tgcgatgttt tttatgatct gagaaagacg ttatgcctga
     2041 attctcattt tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg
     2101 gcagccattg ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca
     2161 tagaaggtca ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattca
     2221 ccggaaacca gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg
     2281 ttacaggtca gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca
     2341 gcaaagtcga ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga
     2401 gccgttgcat accccggaac gaggtaagaa agcggagaga ggaacagaat cagcaggaac
     2461 agcacgccaa cggtgatagc cgtcaacccg gttttaccgc ccgccgccgt acccgccgca
     2521 gactcgatgt ataccgctgc cggagctgca cccaccaggc cagagaaaac gctgctcatg
     2581 gagtcagtgg tcagtgcttt cccgccgtcg atgatctgcc catctttatc cagcaggttc
     2641 gcctggccgg cgacagcgcg gatagttccg gtggcatcaa atactgccgt catcaccagc
     2701 gccagaacgc ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc
     2761 agtgaattgc cgttttcatc gctcaatgaa ggcatggcga aaacgccgga gaaatggacg
     2821 ttaggatcga agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca
     2881 gggactttca gtttttccaa gccgatgatc accgccagac ctaccagtga cataatcacc
     2941 gggaaggtca cgaaatcacc cagcgcaacg ggcagaccat caagcgggtt tttaatcacc
     3001 agaccgacac cattagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg
     3061 tgcgccacac cgtgcggcaa gttgcgcaaa atccagctac ggatacccgt ggcagaaatt
     3121 accgtaaaca gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt
     3181 tgccccagca ccaggctgaa tgcggtaaac accgtcaggg agatggcgca accaatcgcc
     3241 aacggcagat tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtt
     3301 gcaacgaaaa ctgccgcagg cgggaagcct gctttaccca acatacctgg aacgacgatg
     3361 accgagtaga ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg
     3421 cttccacgtt gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc
     3481 gtagacataa gaaaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat
     3541 cccctgccag cgaaacgtta tcttcctgct ccaggtttgc ggcaatttgg tgcgtcgcaa
     3601 aaaagcaaac gtttagctcc gcgcatacaa aacgggtggt aaaaaaaagc aaacgattat
     3661 ccggcgatca tagcggcttt ttcaaccgaa gtttgctgct tttttctctt ttattgtctt
     3721 gacgatgaaa aaccaggcaa ttgatgcgca aacgttatcg tcgatgcgca agattgcagc
     3781 gttatcgagc cgtataccgt tctggattgc gataaaaaat cgtctgagat gacaaaaata
     3841 ttgcggctaa aaagtatact tttacactgg cgaagaaacg tcggtgacat acaattaaag
     3901 catcaacacc aaccggaacc tccaccacgt gctcgaatga ggtgtgttga cgtcggggga
     3961 acccctcctg tgttccagcg ggatagagag aaagacaaag accggaaaac aaactaaagc
     4021 gcccttgtgg cgctttagtt ttgtgtttgc ggataattcc aaaagagctc gatcagtccc
     4081 ctcgcccctt cggggagagg gttagggtga ggggaacagg cagcaccagt gcaaatattt
     4141 accctcaccc cggccccata gttaacgatg agtcagcttg tccttgtggc gctttagttt
     4201 tgttcatctt ccagtaagcg tgcgccggta ccttcttctc ctaagcggtc gcccgggtta
     4261 cgcaacgggc aatcactgcg cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca
     4321 cgcagcgcca ctaaggtatg aatgcgccga tccaactctt ctcgccattg ggacgaaagc
     4381 tgtttccact ctttcgcact taacgtatgc ccttctggca acacgccaaa cgcttcacca
     4441 atggtcgcca gcggaatgcc aatacgctga gcgattttga taattgcgac atatcgcaac
     4501 acatcacgtt tatatcgccg ctgattgccg ctgttacgga tactagtaat caaccctttg
     4561 ctttcataga aatgcagcgc cgataccgcc acaccgctgc gtttcgccac ttcgccaggg
     4621 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
     4681 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
     4741 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacgt gcatattgac
     4801 cagccgctta acattgatgt cgtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
     4861 cggatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgccgcctg
     4921 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
     4981 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
     5041 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
     5101 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
     5161 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
     5221 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
     5281 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
     5341 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
     5401 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcatt
//
LOCUS       DADLSE010000211         5353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLSE010000211 DADLSE010000000
VERSION     DADLSE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.212"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            253..510
                     /locus_tag="JN692_003874"
                     /pseudo
     CDS             253..510
                     /locus_tag="JN692_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN692_003875"
     CDS             complement(560..1510)
                     /gene="uspE"
                     /locus_tag="JN692_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7266029.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1662..2414)
                     /locus_tag="JN692_003876"
     CDS             complement(1662..2414)
                     /locus_tag="JN692_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7266030.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN692_003877"
     CDS             complement(2609..3124)
                     /gene="ogt"
                     /locus_tag="JN692_003877"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7266031.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3135..3800)
                     /locus_tag="JN692_003878"
                     /pseudo
     CDS             complement(3135..3800)
                     /locus_tag="JN692_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3897..4925
                     /locus_tag="JN692_003879"
     CDS             3897..4925
                     /locus_tag="JN692_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7266032.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKLRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKLGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            4922..>5353
                     /locus_tag="JN692_003880"
     CDS             4922..>5353
                     /locus_tag="JN692_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAY7266033.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLS"
BASE COUNT         1380 a         1206 c         1302 g         1465 t
ORIGIN      
        1 agcgatcatt gttttatcag gctcctccag ataattgtcg tcatgccgga aacccctggc
       61 ggggctattt taccgcgaca attcattcag atcatcaata gtcagggaag gaagtagcaa
      121 cattagctaa ctaatcaatg cgttgattgt aaatccagct aagaggtgag gttttcagag
      181 cagacaacgg tgaaacgtca tggtattgtt acgtttaggt aacaagaaat ttgtctgcac
      241 aaggattaca tcatgattat ggcgaaactg aagtcagcga aagggaagaa atttctcttt
      301 ggtttgttgg cggttttcat tattgcggcg tcggttgtga ctcgcgcaac catcggcggc
      361 gttatagaac agtacaatat tccgctgtct gagtggacga catcaatgta tgtgatttag
      421 tcatcgatga tttttgttta tagcctggtc tttactgtgt tgctggcaat cccgttggga
      481 atttatttcc ttggcggcga agagcagtaa gtaaaaaata ggcccgataa ctcgggcctt
      541 gtcagttatt gaagagtcgt taatcgtctt cttcgtcatc cagttcaacg ggtgtctgat
      601 actggtcagg tttaatgacc agcaggtcgc agcgaagatg atcaatcacc tgttccgccg
      661 tgttgccgag gaatgctgct gaaataccgg tgcgtccgac cgtgcccaga accacaattc
      721 ccgcctgtaa atgctccgcc aaatcaggaa tcacctcttc gggcagacct ttttctacgt
      781 gcgtcatgtt ttcattaatg ccgaatttct gccgcagggc tttcattgcc agcaaatgtt
      841 gcccacgaat ggcatcgtta taaacgctcg ggtcaaattc cggcagttca atcgcgatat
      901 taattggcgt taccggataa gcgccaacca gatgaacttc ggtatggttg acttgttctg
      961 ccagttcgat cgtctctttg accagttttt cattgagcgc attatgatac ggctcttcac
     1021 tggcgagatt caccgccacc agcgccttgc ctccttccgg ccacggctgg tctttcacca
     1081 tccacaccgg gcttgggcat ttgcgtaaca gatgccagtc cgttggcgta aaaatcaccg
     1141 cttccagacg gtcatgttgg tgcgccattt ttagcaccaa atcgtgtccg ccgctgatca
     1201 cttcctgaat gatggcttcg aaaggacggt tatgccagac cactttaatt tcaatgggaa
     1261 cgccagcatt gagataatat tttgcctgct cgtggatcca ggctgtacgc tggctgatga
     1321 cgccctgacg catagcggta cgttcgtccg gggagagcag ggtggtcatt tcgtatgaga
     1381 agtcatagat cggcaaaaag gctttaattt tgccaccaat ccgttgatgt aaataaacag
     1441 ctcgccgcaa tgctggttgg tcgtcctggt taggatcgat aacaacgagc atgttctgat
     1501 acatagccat acagggtctc cttacaacaa ctgtcaacgc agtttgtaat taaaagatta
     1561 acccatatct ggtgaatgaa acagtgatga accttctgcc agatcaataa atcagaaaaa
     1621 tttaatgata tgacagaagg atagtgagtt atgcggaaaa atcaggcaac gttacgcgta
     1681 tgaccagcaa gctgggccag cgcatcgtta ttttcgatgg tgatgtattt acctttgact
     1741 gccagcatac cgcttttctg gaagcgaccc agcagacggc tgatggtttc taccgtcagg
     1801 cccagatagt taccaatatc gccacgagtc atcgtcaggc ggaattcacg aggggagaag
     1861 ccgcgttggg caaaacgacg ggacaggttg tagatgaatg cagccagacg ttcctcggca
     1921 tttttcttcg acaacagcaa gatcatgtcc tgatcgcctt tgatttcacc gctcatcaga
     1981 cgcatcatct gctgacgcag attcggcatt ttaccggaca aatcgtccag cgtttcgaac
     2041 ggaatttcac ataccatcga ggtttccagc gcctgagcga agctcgggtg atggccgctg
     2101 ccgatggcgt caaatcccac taggtcgcct gctaaatgga aaccagtgat ttgctcgtcg
     2161 ccttgctcag taattgtata acttttaatc gtaccggagc ggatggcata aagcgattta
     2221 agttcatcac cagccttaaa cagcgtctgg cctttctgaa taggcttctt ccgctcaatg
     2281 atattatcaa gctgatcaag ctcatgttcg ttgagtgtga acgggatgca aagctggctg
     2341 atgctgcaat cctggcaatg gatagcacaa ccgccagact gaatgcgccg tataattcgc
     2401 ttttccggga tcataggtct gctcaagccg taattgatat ttgtcaattt taacatcttt
     2461 ttagggagca agtaagtcta agcaaacctt aacagcagag aattccgata ttagatgtaa
     2521 atatatgtct atctatttga aactccttaa gttgtcaagg gtaactttac ataaaagtgt
     2581 gaacaagctg gcacaaattg tttaatgttt acagcaaaag ataaccttcg tggcgcaata
     2641 accactcttt tcgctgaact ccgcctgcat atccggtcat ggtgccgttt cggccaataa
     2701 cccgatggca aggtacgacg atgctgatgg gattcgatcc gtttgccgca ccaacggcac
     2761 gcgccgcgcc agggcggccc aattgttcag ccagttggcc gtaatgcatt acctgcccgc
     2821 aggggatagt gcgtagtgtt ttccagactt cgcgctgaaa tggcgtcccc cccgtggcag
     2881 tgggaagcgt atcaataatg ctaagattac cggcaaaata ttcacgaagc ttgtcgctta
     2941 aaccacctgg gttggtggca gaaatgcgct catagccttc tttgcgataa tggatgtcca
     3001 gcagctgcac catgcgttcg ctgtactctt cccattcaac cgcccgcagg cgaaattgct
     3061 catcgcaaat cacccacagt ggacccagtg gcgtggcaat tttttcttca agtaatctca
     3121 gcatccgttc tctctccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
     3181 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
     3241 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
     3301 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
     3361 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
     3421 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
     3481 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
     3541 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
     3601 gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
     3661 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
     3721 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
     3781 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
     3841 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
     3901 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
     3961 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
     4021 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
     4081 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     4141 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     4201 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     4261 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     4321 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagcagg
     4381 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcta cggctggcag
     4441 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     4501 ctcaggcact actggatctg gaatataccc ttgttcgtgc gccgattgat ggcattgtcg
     4561 caaaccgcag cgcccataca ggcagttggg tggaaggcgg cacatcgtta gtttcactag
     4621 taccagtcag tgagttgtgg gttgatgcaa attataaaga aaaccaaata gcgggaatga
     4681 agctaggaat gaaggctgag atccgtgctg atattctcaa aggggaagtg tttcacggtc
     4741 acattgaaag cctttcccca gcgacaggag ccagtttcag cttgattccg atagaaaatg
     4801 ccactggcaa tttcaccaaa attgttcagc gtgtcccggt acggattgca tttgacgacg
     4861 caaaggaatt aaagcaactt cttcgcccgg gactgtccgt caccgtcagt gttgatgaga
     4921 gatgatgtct atgcgtaaac atattgcttt cgccagtatg tgtatgggat tatttattgc
     4981 tcagcttgat attcaaattg tatcgtcatc cctaaatgaa ataggtggtg gtctgtccgc
     5041 aggtaaagac gaaatggcct ggcttcagac cagctattta attgctgaga ttattgttat
     5101 tccgctctct gggtggctgt cacgggtatt ttccactcgt tggcttttta ctctatccgc
     5161 gggaatattt actttaatga gtatcgcgtg tggacttgca tggaatattc agatcatgat
     5221 tttgtttcgt gctttacagg gagctgctgg cgcatccatg atccctttag ttttcacgac
     5281 ggcgtttatt tattaccagg gtaaagagtt ggggttggct gctgcggtag tcagtgcgct
     5341 ggcctcactg tca
//
LOCUS       DADLSE010000212         5335 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLSE010000212 DADLSE010000000
VERSION     DADLSE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.213"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..234
                     /locus_tag="JN692_003881"
     CDS             <1..234
                     /locus_tag="JN692_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HAY7266034.1"
                     /translation="EVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILALENKHADY
                     PHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            244..1575
                     /locus_tag="JN692_003882"
     CDS             244..1575
                     /locus_tag="JN692_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HAY7266035.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGLHNGMMLDDAFFGCARNADA
                     IIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGGFLEIAQNARKVV
                     FCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQSGQEVLYITERA
                     VFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFTPMEDA"
     gene            1572..3644
                     /locus_tag="JN692_003883"
     CDS             1572..3644
                     /locus_tag="JN692_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HAY7266036.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSKLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRRVFMDFNRNPEAVPGDLPFTLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNSGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVIAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALVNENGMALVTVKEKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEITELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LNGEIYTQ"
     gene            3737..5014
                     /locus_tag="JN692_003884"
     CDS             3737..5014
                     /locus_tag="JN692_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266037.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
BASE COUNT         1327 a         1260 c         1378 g         1370 t
ORIGIN      
        1 gaagtggtag aaaaagaaca ggtggaaagc tggatggcga accagttagc gcagctggaa
       61 aaatgcggtc cggtggcact ggcgcatatt aagcaactga ttctggcact ggaaaataaa
      121 catgccgatt acccgcacca gctactggcc ggattaatgt ctgccactca ggattgccag
      181 caggcgacgc gcgcctttgc cgaaaaaagt agcgtgagct tccataatca ataaggaaat
      241 tgtatgcgta agataacaac cgccgaggca ctggcggcgc aaattcagga cggtgcgact
      301 attgctatta gcggtaacgg cggcggtatg gtagaagccg accatattct ggctgctatt
      361 gaagcgcgtt tcctgcaaac cggacaccct cgcgacctga cgctaatcca ctcgctgggt
      421 attggcgatc gcgactgcaa aggcactaac cgttttgcgc acgcagagat gctcaaacgc
      481 attattgccg ggcactttac ctggtcgccc aaaatgcagg cgctggtaaa aaataacacg
      541 attgaagcct actgctttcc tggtggcgtg attcaggcac tgctacggga gatcggctcc
      601 ggacgtccgg ggctttttac ccacgttggg ctgggatcgt ttgttgatcc acgcaatggc
      661 ggcggtaagt cgaatgaatg cactaccgac gacctggtag aactgattga aatcgacggt
      721 gaaaccaaac ttcgttatcg ccctttcaaa gtggattacg cgattttgcg tggcacttat
      781 gccgatcctc gaggcaacgt cagccttgaa gaagaagcga ttgatatgga tagctattcc
      841 atggcgctgg cagcacacaa cagcggcggc aaagtgttcg tacaggtacg cgatgtgctg
      901 gaagctggcg cttcaaccaa ctttggcttt ggtattcctg ggggtattcc aggcgtagcg
      961 ctgcgcgaag gcgtgcctta ccaaagttta tggctgagcg tcgagcaagg cctgcataac
     1021 ggtatgatgc tggatgatgc gtttttcggc tgcgcccgca acgccgatgc cattattcca
     1081 tcactcgatc aatttgagtt ctacagtggc ggagggatcg atatcacctt cctcggcatg
     1141 ggagagatgg atcagtacgg taacgtcaac gtatcccatc tcaatggcaa tctcattggt
     1201 cccggcggtt ttctcgaaat tgcgcaaaac gcccgtaaag tggtgttctg cggcacgttc
     1261 gacgccaaag gtagcaagat tgatgtaacg ccagatggct tgcatatcac ccagtcaggt
     1321 caaatcccta aactggttac ccaggtggaa aaaatcactt ttagcgccgc ctacgcacag
     1381 caaagtggtc aggaagtgtt gtatatcact gaacgcgcag tattccagtt aacggcagaa
     1441 ggcgttgaat taattgaaat cgcgccgggt gtggagattg agcgcgacat tctgccgtat
     1501 atggctttcc gtccaattat caagcaccca cgcctgatgg aaagtagcct gtttacgccg
     1561 atggaggatg catgagtcag cttgatgaca ccatccttga tgcgctgacg cacgttactt
     1621 tcccaaaggg ttttgcacag gcagagcccg catgggttgt cacggtagac ggtgttgatt
     1681 acccactctg gcaaacagat gccctggtag tcggcagtgg tgcagccggg ctgcgtgcag
     1741 ctgtggaact taaacgccgc cagcaaaatg tgctgatcgc caccgccggg ttatatatgg
     1801 ggacgtcggc atgttctggt tccgataaac agacactgtt taccgccgct accgcgggca
     1861 acggcgacaa cttcaccaaa ctggcagagg cactggcgag cggtggggcg atggatcacg
     1921 acaccgctta tgtcgaagcg gtaggttctc tgcacactct tggcgggttg caatatcttg
     1981 gtctggaatt accggaagat cgctatggcg cgattcttcg ttatcaaacc gaccatgacg
     2041 aagccgggcg tgcaacctcg tgtgggccgc gtacctcaaa gttaatggtg aaagtgctgt
     2101 tggaagaagt acagcgcctc gccattccag tgttgaccag tgcaacagtg attaaactgc
     2161 tgcatcagcg tgacgaaaac ggcgaagacc gtgtggcggg ggcaatcctc gcgaccggtc
     2221 atcgcgccca taacccttgg gggctggcaa ttgtgactgc gcccaatgtg gtactggcaa
     2281 cgggcggacc gggcgagctt tatcgcgaca gtgtttatcc acataaatgc ttcggctcgc
     2341 tggggctggc gctggaggaa ggtctgacgc taaccaatct gaccgaaagc cagtttggta
     2401 taggcacacc gcgcagcacg tttccgtgga atttgtccgg cacctatgtg caggtgatcc
     2461 cgtatatcta ttccgtggat gctgagggta acgagtataa cttcctcgcg gattactatc
     2521 gcaccaccca agagctggct tcaaacatct tccgtaaagg ctatcagtgg ccgttccacg
     2581 ccacgcgggt aatggatttt ggctccagct tgttagatat ggcggtggcg caagagcagc
     2641 aacatggtcg tcgggtgttt atggatttca atcgcaatcc tgaagcggtg cctggtgatt
     2701 tgcccttcac attagatcga ctggacgacg acgttcgcgc gtatctggaa aataatgacg
     2761 ctctggcacc atcgcccatt gaacggctgc aacgaatgaa tccgctgtct atctcgctgt
     2821 ataagatgca cggttacgat ctcaccacgc agccattgca gtttgccatg aataatcagc
     2881 acatgaatag cggcattgaa gtggatatct ggggacaaac atccctgccc ggttgttttg
     2941 cggtgggaga agtagccgga actcacggcg ttacccgacc aggcggtgcg gcgctgaatg
     3001 ccggacaggt tattgccgtg cgtctggcgc gctttattgg ttgcacacaa aagcggtctg
     3061 ttgagggtga tgtcgcgcag ctggtagcct catctctgaa tatagtcaga gaagttatcg
     3121 ctcaggcact ggtaaatgag aacggaatgg cgctggtaac ggtgaaagaa aaaattcagg
     3181 cgcgaatgtc tgattacgcc ggatttattt gtcatgccga taaagttcgt cacgccaccc
     3241 gtgacgccct gctgctaagt gagtttgtac aacaacacgg tttaaccatc aatcacgcag
     3301 gcgaaattac cgaactattt atgtggcggc atatggcgct gacttctgcc gccgtattaa
     3361 cgcaattgac gcattatatt gacgcaggtg gtggcagtcg tggggcacgg atagttattg
     3421 atccacaagg caaatgccta ccacaaactc gtcgcggcgc aaaagaagaa tggcgttttc
     3481 gctctgaacg tgctgaagac aaaaatcaca gattaacgat tcaatattcg caaggttctt
     3541 ttattaccga agtgaagtct ttacgtatgc aaccgtgtat taacggtatt tactttgaaa
     3601 aaaactggcc agacttttta aatggagaaa tttacacaca ataattttca atatttcctc
     3661 tgtacctgat ttttattacc tattacacgg agacaccaac aaccagggtt tattaatata
     3721 attaaaaggt ttaaacatga ataaaataaa gaattaccgg tggcatatga ttgccctcgt
     3781 atgctttatc actgtaatca attatctgga cagaacggcg ttaggtattg cggctccaac
     3841 gattatggag acaaccggaa taactaaaga gcaatattca tggattgtca gtgcattcca
     3901 gttggcctat acattagggc aaccggtaat gggcttcttt attgataccg tgggtctgaa
     3961 gttaagtttt gcgatatgtg ccgcaatttg gggcctggcg acaatgggcc atgcactaac
     4021 cggaacgtgg tctggtctgg catttatgcg cgccctgatg ggtttcagcg aagcgtcggc
     4081 aattccggcg ggtgtaaaaa ccgcatcaac atggttcccg gcaaaagagc gtggcgtggc
     4141 gacaggtgtt ttcaatatgg gcacctcact cggcgcgatg cttgctccac cgttgattgc
     4201 ctggtgcatt atgtttcata gctggcaatt tgcgtttatt gtttcaggta gccttgcttt
     4261 gctcgcggct ttattttggt tcttttgtta taaagatccg aaagatgcca aacgcctttc
     4321 tgatgaagag cgccactata ttgaatcagg acaagaacag catcttaaaa cagataagaa
     4381 agaaaaaacg tcaatcaagc atatcctcag ccaacgtaat ttctggggga ttggcatcgc
     4441 gcgttttctc gcagaccctg catggggaac cattaacttc tgggtgccga ttttcttcgt
     4501 cgaaacgctg cattttagcc tgaaagaaat tgccatgttc gtctggctgc ctttcctgct
     4561 gggcgatctc ggctgtttag ccagtggttt tgtcgcgaag ttcttccacg atcgcggcgt
     4621 gagtttaatt aactcacgaa gaattacctt cactattgca gccgtcatta tgatgacgat
     4681 tggcctggtg agtattgtcg aaaatcccta cattgccgta ttactgatta gtattggcgc
     4741 gttctcgcat caatgtcttt ctactgtagc agcaactctg ggtggcgatc tgttcaaaaa
     4801 agacgaagta gctaccgcag tgggtatggc aggagcctgt gcgtggagcg gtcagttgat
     4861 tttcaacctg ttcatcgggg cattcgttca cattatcggc ttcgcgccgt tctttattgc
     4921 cctggctttc tttgacatta ttggtgctat tgcgttgtgg acgcttatca aagttaaaga
     4981 tgaagaacca caagtacagt tagctaccca ctaattattt taaataaaaa cgctataatt
     5041 gaggttatgg cgtttttaaa gaagcttgag ttatcgaacc tcgcttacca acgcgttgtc
     5101 agtcatctcc cccctccctg gtgcatcgtt atatccgtga tgagcgattt ttgatattaa
     5161 tatctttcac catccgctat tttcaccact tcagaaaaca aacttatttt tattaaaatc
     5221 aattcttttt ctttgaacat tcaactgcga tatttgtaaa aacattaaac aggcaacaat
     5281 aaaatgacat taccaacaac tatttattca ttccccgcat atttaccccg ttttt
//
LOCUS       DADLSE010000213         5309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLSE010000213 DADLSE010000000
VERSION     DADLSE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.214"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1808)
                     /locus_tag="JN692_003885"
     CDS             complement(<1..1808)
                     /locus_tag="JN692_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7266038.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSAGDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGTLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGN"
     gene            complement(1856..2065)
                     /locus_tag="JN692_003886"
     CDS             complement(1856..2065)
                     /locus_tag="JN692_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HAY7266039.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2161..2535)
                     /locus_tag="JN692_003887"
     CDS             complement(2161..2535)
                     /locus_tag="JN692_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266040.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(2541..3257)
                     /locus_tag="JN692_003888"
     CDS             complement(2541..3257)
                     /locus_tag="JN692_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266041.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(3324..3668)
                     /locus_tag="JN692_003889"
     CDS             complement(3324..3668)
                     /locus_tag="JN692_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HAY7266042.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(3665..4111)
                     /locus_tag="JN692_003890"
     CDS             complement(3665..4111)
                     /locus_tag="JN692_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAY7266043.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(4108..4458)
                     /locus_tag="JN692_003891"
     CDS             complement(4108..4458)
                     /locus_tag="JN692_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7266044.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(4468..4794)
                     /locus_tag="JN692_003892"
     CDS             complement(4468..4794)
                     /locus_tag="JN692_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7266045.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(4791..>5309)
                     /locus_tag="JN692_003893"
     CDS             complement(4791..>5309)
                     /locus_tag="JN692_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7266046.1"
                     /translation="PVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAID
                     DQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSS
                     GQRTEKTFSLLVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTADKPGDAHASGWTL
                     AAKRGRDAGGGK"
BASE COUNT         1110 a         1773 c         1276 g         1150 t
ORIGIN      
        1 tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat aaccagcatg
       61 gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac acgacggtct
      121 tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatctg tgccagacgg
      181 gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac ggtcttcacc
      241 gaggtcagat ggtttatcat gaatccgtca ccggtcgtcc agcccgggtt cgccagaaca
      301 tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactggcctg ttcatcaatc
      361 agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga ggcttcaggc
      421 aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata ctggcgcgca
      481 gactccctgg cctgctcctg attctggtac agcgtgtacc atgctgcagc ccccagcatc
      541 accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg tgagcccacc
      601 gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt tctgttcagg
      661 cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt cccgcgggct
      721 gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt ggcaatctgc
      781 gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt cacaagtcct
      841 gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggccac cccgacggca
      901 accagcgcac ccacagctgt tgccacatta tcaatctgtc cggcaacacc gttcagcatg
      961 ccggagagcg tttttgtcac cccgctggcc tcattcgcac cgcccaccca agccataaag
     1021 gcgttttcca cctttgtgat cccgtcagag accgtttctg gcatggcggc atattcatca
     1081 cgcaatgtcc ccagctggct gattaacgcg ggcacgactt tatccgccgt cagttgacca
     1141 tcgtccgcca tcgccttcag atctttacgg gccacgccca tgcctgcagc cagtgcacga
     1201 atgatccggt caccactttc attgaccgaa ttaaattcct caccgcgcaa cacaccctgt
     1261 gccagcgcct ggctgaactg ggtgatcacc gaacccgcct cagccgtact ggcaccggag
     1321 attttcagcc ccgtggaaat ggcctccgtc accttcagta catcaccggc actgtaacca
     1381 tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga aaaagcggta
     1441 cctgtccgct gactgatatc catcagcact ttctgtgatg acgaaaattc atcggatgac
     1501 tgtgatgcct gtttcagacg ggcattcacg gaactccact catcagccag tgaaatcagg
     1561 tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagccga agcgcggatt
     1621 tccgtcaact ggctgttcag ttctgccagg gcacgtcgct gctcccgggc tgccgcagcg
     1681 gcctgacgcc cgccattctg cagggtccgg taatattcac tgcccatacg ggaagcccgc
     1741 tggatctccg actggaatga ctgtgaattt gccgaaattt tgataatcag ttcacgtaac
     1801 gtcgccattc acctttctcc gggcgtaaaa aaaccgcctc agcggttctc atcattcatg
     1861 actgtgctgc aaagctcagc gcgtcttcca gcgccgcaaa cggatccacc tccggcttat
     1921 cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc ccctgtgccc
     1981 cgaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca cccagcgggg
     2041 acaccctgtc aaactccatc cacatcatcg ctttgctcgc actcaggctg tgccgcagtt
     2101 cggataaggt gcgccccaga cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg
     2161 ctactttttt ctggccgact ctgcatcagc gatcagttcc agtgcctgac gcagcaaccg
     2221 ggcatgtacc ggaccataga cggccagcac ctgctcacgg tcgtccggag tgaacacccg
     2281 ttgcaggtca gtatcacaca ggacatcgca gaacagcgtc acatccgctt ccaggttacg
     2341 gcgggttttc gccaccaccg acagggtatc gtcatcctct ccatcaccat tgagcacttc
     2401 ctgccacaga taccaggcct ctgccgaagg ctcccgcagc accacgctga catttcccca
     2461 ttccggcacc ttcaccgttt tatgacggaa ccctgacagt ctggccagcg ccagtgtttt
     2521 cagatctttt gccataagcc ttatccgccc gcaccattaa ccgttactgt acacgcatca
     2581 gaggtaatgc tctgcggctg ttctgcagaa tccgttacct cgcaggtata agccccctta
     2641 tcacctgact gcgtattggc tttactgaaa gtgtcagtag tctgtccctc taccggctga
     2701 ccatccttct tccaggcgtg tttataaggc ggcgttcccc cgttgacact gactgacatt
     2761 gtcagcagcg caccggtatt cacggtaagt gtcttctcca gatttttcac aaacgccagc
     2821 ggtaccacat aggacaccgg tttacccttc aggcgaagtg aaaacgttgc agccaccacg
     2881 ccgttggtac cggatgacca ggtgtgctga cgcacttccg ccaggaactt aaagccctta
     2941 ccggacggaa actgcacctt aaacgcatac accgtgtcat tgtcataggc atcacgcagg
     3001 gcgttctggg cctgattcag ataaaaatta cccgacatgg aaatctcgga cgacgccccc
     3061 agaccgttga tgttctcctg ctctgtggag cagagcgtgg tcacatcaat atcctgtttc
     3121 tgaccggcgg tgaactggac ttccttgatg gtgcagtcca ggcgcagata ttccgcctta
     3181 tccatagttt cagcagtcgc cggggcagat gaaatcatca cctgcgtcag ctgtgagcgt
     3241 tcatacaaag cagacattct gcctcctgat aataaaaaac ccgcacgcgg cggggtatgg
     3301 gttttgtaga aaaaaagaaa aagtcacacc gtgacctgaa actccaaggt tgcacggtaa
     3361 cagcggtttt ccggaatata gtcctgcatt tcactgacgg atcccggggc cagcagcatt
     3421 atggcttcac gggcgtcctg acgtatctga cgcgcctgcg tcacagtccc ggcataaacg
     3481 tctatctgca ccgacactga ggactccgcc tgcccgccca tcacgtccgc agacaccgat
     3541 gaaatcaggc tgaaaaccac ccacggaagc gccaccgacg gcctgccatc cagcaggggg
     3601 accacatacg ggtacacctg cccgccggca agatgcgcca gatgaggata caaatccgcc
     3661 tccgtcatcg tctcagtacc tcatcaatgg cccggttcat ccgagcaatc gccacctgcg
     3721 ctgcctgttc actgcgcaca tcaaatgccg gacgcacaaa cgggtgcggt ggcatattca
     3781 cggtccccat ttccacaaac cgccagtaga aagcattgcg cgggttatcc gccttcatgg
     3841 tgttatcgct gttaccggtg tccggattaa caccacggat atggacaccg gattccatcc
     3901 cgccatcgcg ggagcgccgg gaaaggacca ccacattgcg gcgcagtttc cccctgcgta
     3961 ccggtgcccg tgacaccact tcttctttca gcacattcgc ccccgcacgg gttgcctcac
     4021 gcagcacccg gttattttcc gcaccactca gaagctgcaa atcgcggctg atgtcctcca
     4081 gccccgaaaa atccagcagg gtttcgatca tttttcccct cccagccgac agagaatttc
     4141 cagacgccca ccggtcgcat ccggcacggg cagcccgaca acgttcagga tccggtcacg
     4201 ccatggacca ctcagcacat gaagtcgtga cgctgccgtg atttcccggc cggactgacc
     4261 gcgcacccag atgcggattt ccgcctgcgc catttccgca ccggactgca tccgctcccg
     4321 gctgctcctg ccacggatat ccgcatgaat tttcccgcat gacacccatt cttccgtcat
     4381 ttctccggca gcattacggg ttaacaccgg gttcagaaca cttatcatct gtgtcagacg
     4441 acctgcagat attgccattc cccctcctca taacaccgtc ggacaacgca aatcgtaaat
     4501 cagcacggaa acagaaaacg gcagctcccc ctgaatcagt tcttcccgct ccgcaagatc
     4561 cggattccgg tacagcatcc cggtcagtcg catggcagcc cccttcatcc gggttaatgc
     4621 ctcgcccggg atcagttcac cgtcctcacg aatcacttta tcccggctgc cctgaatgta
     4681 ggccagcagc acggcggtag cctgacgaac cttgtccatc agcatgtcat catccgcgtc
     4741 atggtcgaca cgcagatgtg ccttgatctc ttccagtgtc agtaatgccg tcattttccg
     4801 cctcctgcat cccgtccccg tttggcagcc agcgtccagc cagatgcatg cgcatccccc
     4861 ggtttatcgg cggtcatact gttgcagtgc cacagcgagc ccccccacgt caccgtatca
     4921 ccggggtggt aggtttcacc ggctctgaac acaccgcggt agagcatcac cagcagggaa
     4981 aatgtttttt ccgtacgctg gccactgctc tgccggacca ccacagagaa caaccgttca
     5041 cccgtcatgc tgacgtcaat atccgccacc ccgtcaacca ggcattccca tccccgcatc
     5101 ccgtgcgttt tttcatacgc ccgccagagt ccgccctggt gtgtggcata cgtgccccgg
     5161 ggaaaggatt tttgatcgtc aatggcgggg agtatttcca gtgccgtggc atcacgcccg
     5221 tcctgcggag ccggcagggc actcaccgca tccagaaccg ccttctgcag aacatccgga
     5281 tcgtagtcac gaccatcacg cggaacagg
//
LOCUS       DADLSE010000214         5286 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLSE010000214 DADLSE010000000
VERSION     DADLSE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.215"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..411
                     /locus_tag="JN692_003894"
     CDS             <1..411
                     /locus_tag="JN692_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7266047.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            431..742
                     /locus_tag="JN692_003895"
     CDS             431..742
                     /locus_tag="JN692_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7266048.1"
                     /translation="MTLTLNELLLKLDRYRVIVVDDLGYVKRDSAETGVLFELIAHRY
                     ERGSLVITSNHPFSMWGSIFVDETMAVAAADRLIHHGYMFELKGESYRKKTAKAVTSV
                     T"
     gene            complement(911..5005)
                     /gene="sepA"
                     /locus_tag="JN692_003896"
     CDS             complement(911..5005)
                     /gene="sepA"
                     /locus_tag="JN692_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7266049.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSWADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1297 a         1330 c         1230 g         1429 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cggaagcgta tctgcgccat attctgagcg tactgccgga atggccctcc
      361 aaccgtgttg acgaactcct gccatggaac gtggttctca ccaataaata agcgtcaata
      421 cggtgctccg ttgacgctta cactgaatga gctgctactg aaactggatc gctaccgggt
      481 gatagtggtg gatgatcttg gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt
      541 cgagttaata gcgcatcgct atgaacgtgg gagcctggtg ataaccagta accatccgtt
      601 cagcatgtgg ggcagcatct tcgtggatga gactatggcg gtggcggcag cagaccgact
      661 gatccatcac ggatatatgt tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa
      721 ggcagtaaca agcgtgactt gatgtcgcac tgaaggggcg gtcagtataa ttggcgccag
      781 tcggcaaaac tagttgacgt ctaatacctg gacggtcact tctgagaaaa ggcatttaca
      841 cagaatctta aacaggctca cggagttctt tcagaaaaag gcctgccgaa tggcaggcct
      901 atcccattcg tcagaaaaca taacggaagt tggcgtttat cgcattatcc acattatatt
      961 tcccgaacgc tgatttctcc agctccagtc ccaaacgcat attatcctta atttccgcat
     1021 tcatccctac cgtcatcagc atcctgctat ctttttcacc ttcgaaacgt ttctctccgg
     1081 aagcatcctg cagaaccgtt tctccgttcg ccagcaggtc gaactgataa cccagcccgg
     1141 ctcgagctgt gattttccag tcgtctccgg agaaggctct tcccacgtca acaccagtac
     1201 ggccaatcag tgggttataa tccttgtctt tcatgctcag agccattccc cggtcttccc
     1261 agctaaaagc ttttcctgat acagaaccgt aaaccagctc tatctgtggc tccacccagg
     1321 actctttcgt caggtggtaa cgataaccaa cttcagcacc ggcataccag gaatgagagc
     1381 tgtagtcttt tgttcccagt gaggcaaaat tcgccgtgtg ctgattatca tgatggagat
     1441 atttaccaat caggtcaaaa taagctccgg aattaaagag tgcagaggca tacagaccgc
     1501 cacccacgga tttgtttttt ccactgaatg cgtggctgct tgcattgctg tccgtatagg
     1561 tcagcaatgc ccccgtaaat aaatccacac cgtccagctc atgctttctg tcgacaccaa
     1621 tctgaacgtg agtgtagtta tcgctgtagt caccatctgc cgaaccggta ccattcatta
     1681 tgcgtgccca gacaccggca tccccctgag tatcccgcag gtcacccata cgtttgttca
     1741 ggttattgac ctctgtaagg aaggacttat aacctgatgc cataaagtct tttgcggctt
     1801 tcgaggctcc ggcatcagcg gtagtctgat atcctgtcag cacccatttt gtggcatcat
     1861 ccgttttttc cgtgctgatt actggcgtaa cgttactgaa accaatctgc tgcgtaccgg
     1921 cagtgaacgt tttctcatca ctccctttcg gtgcggtaat taacgtcacg ctcatttcac
     1981 tggctggttt gtttaaaaag tcaaccagga cgctattatt actgcctgac agtttgtctg
     2041 tgacattcaa ctggtcagcc cagctgttgt tggtatgcat caggaacgtg gtattatctg
     2101 cagaaagctc ttttacttgt aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg
     2161 agcccttagc cttcagtgca ccggcctctg attttccgtc agagtaccac agagcattga
     2221 tcatatttac cgtactctgt gcccccgtaa tactgcctct cagaacagcg ttatatccct
     2281 tcattaagga gctgaataac ggagagtccg tcttccctgc atcagcatca ctgtctccca
     2341 gattaatggt tgccgcagtg gttcctgcat gaatatccga actcagatag cccatatttt
     2401 taacagaaaa gctggcgtta tcttcgaggt taatcccttc tgttgtagaa attacgggtg
     2461 agtaataccc ctgtttctgc gcagaaggca tccccgtcag ggtcagagaa gcattttctt
     2521 ttacatccac agccccggtg ctgaaaatac cgctctgagc cgtaagtttt gctccttttc
     2581 cgagggtcag gctgctgttg acaaagctgc caacacggtc aaaaacagca ttctgagagg
     2641 tcacactcac ggtgctatca taagcctcaa taccaccggt gaatctctcc atgatttgca
     2701 gggatgattt attttccagc gtaacatttc cgctgtaatc actctggtca ccaactgtgg
     2761 ttgcagtggc actcccttca acagaggaga ccgcattacc ggttccgtca ctcctgtcag
     2821 taaagacacg gctacttccc agaatgactg tacttccatt ggtggcgtta atatctccct
     2881 gcatcttgcc gtttctggac agatggaatg ttgccgcatc gagtttcagt ttttccagcg
     2941 taaactgacg ggtttcccag tcgctctggc tggaagaagt cgtcgttcca gcatgaataa
     3001 ctggatgacc ctggaatatc agcgtgccgg acttcttggt tatcgaacct tctggcaggt
     3061 taactgaacc atcaagagcc agtacatcct tacctgagag ctgcggaata ttcacatcca
     3121 tattgccgtt tagttggccg tgatacatat aaggctggct gctggcctca attttttgtt
     3181 gcttcacagt atcaatggct ttattacgat cttttccgac attctgccag acactgctgt
     3241 tgttcagatc ggagaagaac ggggaatatg aactcgcttt caggatgaaa tattgcttgg
     3301 tagaactgtc ataatatagg tctcccggcg ctcctctccc cccaaaaata ctgacagtat
     3361 taaccggtac attgatgtca ctggctttta atgtctgaag gtcgagtgtt accgttgatt
     3421 ttttctcact gttattggta atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg
     3481 tcagattatt accattcagt tccagacgtc ctccacggta tccccacgaa atattatccg
     3541 gattaacttg ctgtgagtca gaaagcacaa ctgtcggacg accgctggca ataccaacag
     3601 agctgaaagc ctgtaccttt ccgtcagcat ccgcctgctg gttgagaata acggtaccgt
     3661 cccccacctt gagtccgcct gcattcacac cggtcccgtt tactgtcagg gtaccttcgc
     3721 cagttttatg caggttatca ccggcaactc cgttcacctg ccacagaacg tgggttcctt
     3781 tatcggtgat aatcccgccc cccatccatg tctggccgtt cagggcggac accctgtaat
     3841 cgccggcaaa ctgcagatac ccagctccct gattaacgga attctgcaga acgacctcgc
     3901 cattattgcc ggtgaacagg aggttttttc ctgcgttcag atcatttcct ttctttccat
     3961 gcatatccca agtcgtgttt ccctgagtca gtgtgccggt gccattagcc gcatcatatt
     4021 tccactgcag tactccttca ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg
     4081 gttgctgacc aaggaaatcc tgagtggtga cgacccagtt atttccatag ttagttcctg
     4141 aggaaagcac gccaatcagt acccattttt tttgcaggga atcataagca aacagggggg
     4201 agccgctgtc ccccatattc agataattag ccagaggtcc attcgcggga ttaaaaatgt
     4261 caccggtctg agctgtaatc atctgtccgt tgttatagga gttcagcgcg ctgacggttc
     4321 ccccaacaag aaatccgcca ttggtataca cttgggtgcg attaccattt ttgtctttga
     4381 cgtactgcat ccccccccca aggcgataga attcagtaaa gcgtccgccg gcctgatagg
     4441 caccgctcac agccccgatg tcagacactt ctgccggcgc cacctctgtc accagtttac
     4501 tcaggcggcg ggttttaata tcaagaccgc tattattatt tgtaccaacc gcagtatagg
     4561 tgtttttggc gtatccgaag ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca
     4621 catactgagg ggagtaaagt gtatggtctc ccggaggcag gctaccggta gtaatagtgg
     4681 cactactgaa atcagccatc ggggctttat caagtttacc gaccagattg ccctgcttgt
     4741 cgtaaatgga aatattcgtg gtccccggcg taaactgacc tttattctcg gcaaagtcgc
     4801 ggaatatctg ataagggatc tctgcactga ccgttgctga cagagctggc catgccgaag
     4861 aaagtgataa agctgcaaca gacgtaagaa taacccgacg tgaaagtctg cgatggcttt
     4921 tgcaggtaac cctgcgggcc agttcggaga cagcaatcag gcttttggtt atatggcaat
     4981 acttaagata atatattttg ttcatataga aacatcaacc taattttgac aaagattaat
     5041 tacataggat gtagatggag cagtccgaga ttctactaat atcaattatt aagcatctgt
     5101 tttttagttg gtgtaatata atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg
     5161 caagatgtta tttttttatc attttgagcc agtttggctg ggaggatgct cttctcttaa
     5221 gcttcttgct caactttaaa aatagttatg ccagttcggt atcgataagg ggcagtttgg
     5281 atatcg
//
LOCUS       DADLSE010000215         5169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLSE010000215 DADLSE010000000
VERSION     DADLSE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.216"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            11..596
                     /locus_tag="JN692_003897"
                     /pseudo
     CDS             11..596
                     /locus_tag="JN692_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            609..1367
                     /gene="chbG"
                     /locus_tag="JN692_003898"
     CDS             609..1367
                     /gene="chbG"
                     /locus_tag="JN692_003898"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7266050.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN692_003899"
                     /pseudo
     CDS             complement(1414..3665)
                     /gene="katE"
                     /locus_tag="JN692_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            3848..4111
                     /gene="cedA"
                     /locus_tag="JN692_003900"
     CDS             3848..4111
                     /gene="cedA"
                     /locus_tag="JN692_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7266051.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            4388..5169
                     /locus_tag="JN692_003901"
                     /pseudo
     CDS             4388..5169
                     /locus_tag="JN692_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1322 a         1131 c         1309 g         1407 t
ORIGIN      
        1 tctgaatctg ttgccgtgtt cttatttgct ttattacttc aagcaaaaag agatgctggc
       61 tattgaaatg ggcgaatact acaaaggcgg cgcacgagcg caggtagtac agaaagtcga
      121 gaaacaactt tttgagctgt ataaaaaccc ggagttgaaa gttaagccga aagaactgga
      181 acagcgcggt ggggcttatt actctgatgc agcgtgcgaa gtgatcaacg ctatctacaa
      241 cgacaagcaa gctgaacatt acgttaatat cccgcatcat gggcatattg ataatattcc
      301 ggcagactgg gcggtagaaa tgacctgtaa gctggggcgc gacgccacat ccgcgcatta
      361 cgcatttcga tgataaagtg atggggctga ttcacaccat taaaggcttc gagattgctg
      421 ccagcaacgc cgcacttagc ggagaattta acgatgtgtt actggcgcta aaccttagtc
      481 cgttggtgca ttccgatcgc gatgctgagc tgctggcacg cgagatgatt ctggcgcacg
      541 agaaatggct gccaaatttt gccgactgca tcgcagagct taaaaaagca cattaaccga
      601 ggctgattat ggaacgctta ctgattgtta atgccgatga ttttggctta agcaaaggtc
      661 agaactacgg cattatcgag gcctgtcgca atgggattgt cacatcgacg acggcgctgg
      721 tgaatgggca ggctattgac catgcggtgc agttgagtcg tgatgaaccg agtctggcca
      781 tagggatgca ctttgtcctt actatgggca agccactgac agctatgccg gggttaaccc
      841 gcgatggtgt gctgggaaaa tggatctggc agttggcaga agaagatgct ttaccgctgg
      901 aagaaattac tcaggagctt gccagtcagt atttgcgttt cattgagcta tttggacgca
      961 aacctacgca tcttgatagc catcatcatg tgcatatgtt cccgcagatt ttcccgattg
     1021 ttgccaggtt tgcggctgaa gaggggattg cgttgcgcat cgaccgtcag ccgctatcta
     1081 acgctggtga tttaccggct aatctgcgca gttcgcaggg attcagtagc gcgttttatg
     1141 gtgaagagat tagcgaggca ctgttcctgc aagtgctgga tgacgcaagc catcggggtg
     1201 atccttcgct ggaggtgatg tgtcatccgg cgtttatcga taatactatc cgtcagagtg
     1261 cttactgttt cccacggtta acggagctgg aagtgctgac atcagcgtcg ttgaaatatg
     1321 caattgctga gcgtggttat cggttgggta gttatcttga tgtgtaagta aggtggtaag
     1381 gtcactcccc aggggacgtg accttatcca tatttacgcc ggaattttgt caatcttagg
     1441 aatgcgtgac cacacgcggt gttctgccat cagcgttaac agttcatcca taaaactacc
     1501 gtcggcgctg tcagcttcca caatcccttc ttcaccctgg tcagctacct tgattgttgc
     1561 tttaaacttg cgcgcgtctc ccgccagcgc aatcggttta aggtgtttgt aggcttccat
     1621 cagatagtag ttggcatcgc cgttgtcagc gatatccgcg atattgccgc aagggacaat
     1681 gaccgcatcg accgtcagcg aaggtgcacc ggcaaaggta gcggctatag gcaacaccgt
     1741 accgtcatcc gcagtcactt cacccactcg ggagtagagc agtttggcat gaacgccttt
     1801 ggccttcagg gccttgagaa tggccagaag gtctgccgat ctcacttcat cattaagtaa
     1861 aatcgctacc acgcgacctt tcacatcacc gtcaggaatg gcgtacaaac ttaaggatgg
     1921 atcctttttc agaccgttga cgtccggagg tggggtgata ttcagctggt cgtcagtcag
     1981 ttcgataccg agatttttcg ccaccgcctg ggccagagtg agatcaatat gcgccagctg
     2041 gtcaacaacg cgctcacgaa tatacggacg aacgactttg cttaactcaa aactgaaacc
     2101 atcgacaata tggcgctgct cgaatggcgt ctgacttagc cagaacagac gcggatggga
     2161 ataatattcg ccaaacgatg ggctgcgctc gcgaacttta ttgccttcca cgcgctcctg
     2221 gtatgattca aaaccgccgc gtttcggccc cggtggtgtt tcgcgcggcc agttatcgtt
     2281 gatcgagttc ggttcataat tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc
     2341 acgctggaaa ttatggtaag ggcaggtcgg gcggttaatc ggaatctcat ggaaattcgg
     2401 cccaccaaga cgactgattt gtgtatcggt ataggagaac aaacgtccct gcaacagggg
     2461 atcgttggtg aagtccagac caggaacaat atgacctgga tggaatgctg cttgttcgtt
     2521 ttcggcaaag aagttatccg gattgcgatt gagcaccatt ttgccgacac gctgaacggg
     2581 caccagttct tccgggatca gtttggttgg atcgagaaga tcgaagtcga acttgaattc
     2641 atcttcttca ggaatcaact ggaagcccag ttcgtattcc ggaaaatcgc ctgcttcaat
     2701 ggcttcccac aactcgcggc ggtggaagtc cgggtcacgt ccggtgagtc atcccaaacg
     2761 agtgaggctt tacctgccag tggtttccag tggaaacgta caaacgttgc cttcccttcg
     2821 gcattaatca ggcggaaggt gtgaataccg aagccttcca tggtgcggta actgcggggg
     2881 atgccgcgat ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca
     2941 taatcccaga aagtatcgtg ggcactttgc ccttgtggaa ttgcccagtg cggttctggt
     3001 tttaccgcat gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg
     3061 ttattgccaa cgaggtcaaa aataccctct tcagtataga acttggtggc aaaaccacgg
     3121 atatcacgca cggtatcggc agagccagcg ccaccctgaa cggtagagaa cctaacaaat
     3181 actggggtga ttttgttcgg atctgagagg aaatccgctt tggtgatatc gcttaagctt
     3241 ttatatggct ggaaataacc gtgagctgct gatccgcgtg catgaacaat acgttccggg
     3301 atgcgctcat ggtcaaagtg ggtgattttc tcgcgcagaa taaaatcttc cagcagcgtt
     3361 ggaccacggt taccggcacg cagtgagttt tgatcatcgg cgatgcgcac gccctgatta
     3421 gtggtcagcg cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg
     3481 ttacgtgtat caggggcttt caggctccca ggggcggtag gttgtgcacc tggcggtgtt
     3541 ggttcagccg ctggacgatg agatccgtcc tcaggtgcca gtgagtccat ccccggtttc
     3601 gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc
     3661 gacattgaac tcgtctcctt aatttattac tgaaagggcc gctgttttgg tcaaaaaact
     3721 ctctaactat agaccaccag ccagatcccg cttcggagac tattctggaa acggtgttat
     3781 gaaatacacg caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctaat
     3841 ttttaatatg cgtttagtga agccagtaat gaagaaaccg ctccgccagc aaaaccgcca
     3901 gattattagc tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga
     3961 ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatgggaga
     4021 gtcatttctg cgctcaccgg cgtttactgt gcctgaatct gcccaacgtt gggcaaatca
     4081 gatccgccag gaaggggaag tgactgagta aggattacat acctcccggt tttgccggga
     4141 gttacatgac ctgaaattct gcatttcgtt gctacattca ttcaaaccac ctgtaacaaa
     4201 aagctgcctt tgacgagcta ttgctataaa taagtgatct tttttcttta attgtttatt
     4261 tattttaaaa tggttaagtt ttgctaatca tgatgagcta tttttgatag ttcatcctgt
     4321 ggcgtcgctc ttaaacttat attgtaaatg acttagattt aaataagata ataattttgt
     4381 taacaatatg aggcgggaga tgttttctac aaacttaata cagagtaatt atggtgattt
     4441 aaatattaaa agcctggctt tcgactcttt taaagaaagg cttgagtcta ctatggcggc
     4501 actcactttc tttatatcaa cgggtcaatg tgactgcgat gaaatagcag agtcgaactt
     4561 caatcgtatg attgcctata tgtctaacat taactatgat gcctcaaagc taggagcgcc
     4621 agcacttagt tttgatacgt atctgcagga caatgttaaa tatagagtga taattaataa
     4681 tctttatggc agtgagtttc ggataagaga tattaatgag aattttgttg ggatggattt
     4741 tacctgtgaa ttccgacccg aaaaaatgac gaatttgatc aatataaaaa ataggttggt
     4801 tattcattat ttaaggacta tatactatga aaaaaattat atacatcctg ccgggagcat
     4861 ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata atatcaatgc
     4921 ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt ggtgtgcttg
     4981 aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat gaggttttaa
     5041 gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg gacatttttt
     5101 tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa
     5161 gtaaagcaa
//
LOCUS       DADLSE010000216         5153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLSE010000216 DADLSE010000000
VERSION     DADLSE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.217"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            135..554
                     /locus_tag="JN692_003902"
     CDS             135..554
                     /locus_tag="JN692_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HAY7266052.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRREFETS
                     ELIRAYGELKRNETPERHTQGHSEKPHDQQIERILRELNELKQCLTLMLEDKQAQDTE
                     RRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            635..1054
                     /gene="exc2"
                     /locus_tag="JN692_003903"
     CDS             635..1054
                     /gene="exc2"
                     /locus_tag="JN692_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HAY7266053.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
     gene            complement(1279..1602)
                     /locus_tag="JN692_003904"
     CDS             complement(1279..1602)
                     /locus_tag="JN692_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HAY7266054.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1886..2080
                     /locus_tag="JN692_003905"
     CDS             1886..2080
                     /locus_tag="JN692_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7266055.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
     gene            2268..2639
                     /locus_tag="JN692_003906"
     CDS             2268..2639
                     /locus_tag="JN692_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266056.1"
                     /translation="MSKKQSTGRSQRRRRFSIGSAPLTSITKIDAQARGGETRQDYKD
                     TRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISARCRSFAPSWAVCT
                     NPPFSPTAAPYPVTIVLSPTR"
     gene            2567..2725
                     /locus_tag="JN692_003907"
     CDS             2567..2725
                     /locus_tag="JN692_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7266057.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLAPLAAAVGNLVSRAMM
                     AQMVQQSS"
     gene            complement(2922..3059)
                     /locus_tag="JN692_003908"
     CDS             complement(2922..3059)
                     /locus_tag="JN692_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 lysis protein"
                     /protein_id="HAY7266058.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            3107..3448
                     /locus_tag="JN692_003909"
     CDS             3107..3448
                     /locus_tag="JN692_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HAY7266059.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKILYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(3445..5010)
                     /locus_tag="JN692_003910"
     CDS             complement(3445..5010)
                     /locus_tag="JN692_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="HAY7266060.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
BASE COUNT         1457 a         1291 c         1062 g         1343 t
ORIGIN      
        1 aaaaaaacag ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa
       61 acattaccgt tctcagacct cattatgttt tactgaaacc atgagatgag acacctatgg
      121 gacactgtaa ctttatggca tggcacacac tccgggacgc actgaaaatg actggaagat
      181 cgcgttcaca gttttaccgt gatatgcgct caggtcttgt cagttaccgt accagccagg
      241 acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac actcaaggac actcagaaaa acctcatgat cagcagatag
      361 aacgcattct ccgggaactg aatgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acggaaacag ctacagaatg
      481 agatagccca gctcaggcag gctctggagc aggaaaagaa acggggattc tggtccaggc
      541 tgttcggtca ctgaacgcgc tcagaaactg atgataagat aatcttcgga taataactca
      601 ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa acaaactttg
      661 tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt
      721 ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc
      781 cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt tttcggcagt tctgggatat
      841 gggagctaaa gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcaaca
      901 gtttcacagc caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga
      961 ttaccgcagc aaagacctta ccccgaaaaa atccaggctg ctggctgaca cgatttctgc
     1021 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     1081 tacgcaccac tgaaacgcgc tgaacgcgat tctgacgcag acaccgcaaa aagagatgtt
     1141 ttaccccgga aaaacaggaa ggatgcgtca ggatcgtttt cagctctctg catagctatg
     1201 catgaaagcg aatggtgatc actttgggag cttacggtgt tcataccgtc agttttcgac
     1261 agttttctct ccgggaagct aatctgccat aagcctggat aacagggcac ggtgatactc
     1321 cgtaatagcg atcagcacct ccgcatactc cctgtccccg acacgcttgc cgtcgataaa
     1381 gagttttttc tgtaacgccc ccagtctcat aacttctttc agtacccgat catcagtcag
     1441 tgagttaact ttcttaccga gagctgccgc ccgtaaaaaa ccggaaacgg tcttgccgca
     1501 ttcagcagcc ctttttctga tttcctggtc ttcttctgcc gtcagacgaa cagggcgact
     1561 gatagcccgc ctgcgcgtat tacttccact tcgttttgtc ataaaacatg gctccgtatc
     1621 tgacatgggt gtcggggcaa agccctgacc agggtaattg taatagcgtg catgtatgcg
     1681 cggtataaca attacacatc ctgtcctgtc agcaagttcg aattaccgac aaaggcacgg
     1741 cagttttttg gcagtgacag tattgtcagg cagaaatacc cgtcaaagcg tcaaacgaca
     1801 gaataccggc ggtaaaacag tcgaaaagtg gtacgcgata taatcacaca acgcagatga
     1861 cagacagaac ggagaaaacg aaaagatgaa caagcagcag caagccgtac tcaacatggc
     1921 aggattcata aaaagccaaa gcctgacgct gctcgaaaag ctggacgcac tcgatgctga
     1981 cgaacaggct accatgtgtg agaagctgca cgaactcgca gaagaactcc agaacagcat
     2041 ccagacacgc tttgaagtag aaaatagcac agagagataa cgctcaggac aggatgccga
     2101 acggcacaac agagcgtaag gagaaaatgt cacataaggc gctcttccgc ttcctcgctc
     2161 actgactcgc tacgctcgga cgttcgactg cggcgagcgg tgtcagctca ctcaaaagcg
     2221 gtaatacggt tatccacaga atcaggggat aaagccggaa agaacatgtg agcaaaaagc
     2281 aaagcaccgg aagaagccag cgccgcaggc gtttttccat aggctccgcc cccctgacga
     2341 gcatcacaaa aatcgacgct caagccagag gtggcgaaac ccgacaggac tataaagata
     2401 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2461 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2521 ttggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2581 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2641 acacgactta gcgccactgg cagcagccgt tggtaacttg gttagcagag cgatgatggc
     2701 gcaaatggtg caacagagtt cttgaagtag tggcccgact acggctacac tggaaggaca
     2761 gtatttggta tctgcgctct gctgaagcca gttacctcgg ttaagcagtt ccccaactga
     2821 cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacaggc agcagattac
     2881 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac agagatcccc
     2941 gtcaatttag aagaagatga tggagccaca gtccctccct gaacatcacg tatatagtta
     3001 gcctgacatc caacaaggag gtttatagcg aatattccca caaaaaatct tttcctcata
     3061 actcgatcct tataaaatga aaagaatata taacgaggtt taatttatga gtttaagata
     3121 ctacataaaa aatattttgt ttggcctata ctgcgcactt atatatatat accttataac
     3181 aaaaaacaac gaagggtatt atttcctagc gtcagataag attctatacg caatagtgat
     3241 aagcactatt ctatgcccat attcaaaata tgctattgaa cacatatttt ttaagttcat
     3301 aaagaaagat tttttcagaa aaagaaaaaa cctaaataat gcccccgtgg caaaattaaa
     3361 cctatttatg ctatacaatc tactttgttt ggtcctagca atcccatttg gattgctagg
     3421 actgtttata tcaataaaga ataattaaat acccaacacc tcatttatag tattaagttt
     3481 attcttatca ataaaggcac atagaatgcc tgtaacaata gcaatccccc agatacctaa
     3541 tgtagttcca gcaagcacac taaaaagtaa aacaacaaca taactaactc cagcatctac
     3601 agctttcttc tctaatgtaa gaaatagcgg cttccagtca cctgtatcct taatttttag
     3661 gatatcagat accacatcat atccaaaaga aacatgtccc gtaatcttca agtacttggc
     3721 aaactgatct aaatgcttag cccagtcttc atacttaacc gcctccagtg cattgaaaat
     3781 tgcatcacgg tctgctttgc tgaatttctt atttaataca tccttgtatt tctcaaaagc
     3841 agctagagct tcattcacat tgcttatttt cttaccttta gatttatcag caagttcctg
     3901 tgccattttt gaatattttt caccatattt ttcagatagc gtttgataaa agctaactgt
     3961 tgcatccaca gcatccttaa tctgcgagtt caggagatta gtctgtgctg ttttcaaatt
     4021 atcttcagca tcatgaatac gtgaaactgc tgtacttcgc ttattattag cctgagaaat
     4081 agcctcttgg attccattta tctcagagct aatctggtta agacgcgttt ctgatgctgt
     4141 tacctgcttt tgtttttcct ccatctcatc accagctctc gcccgtagtc tggtggcctc
     4201 aaaaaaagga cgactctgaa gcggatcatt cgccctagga gataaaagtt taaccctttc
     4261 atccagttct ttatatttag cagatgcctg atccaactca tttcgttttc cggacagcgt
     4321 attcgtttct gcatcacggg catgaatact ggagcttaaa cgggtattga gcgtattaat
     4381 ctcttcatcc actttcgcca gctcagattg tgcagcagca agattttttt gtgccacctc
     4441 cacagcccgg gcctcttcac tcaatgctgc caggcgtttc tcttcatcct cagccagttt
     4501 caattggcgt tctgtttcag cctgctcctt ctctatctct ttacgtcgtt gttctgcttc
     4561 ctgaaaagcc ttttccgcag cttccgcttc tttacgggct ttttcttctg cttttgcaag
     4621 gcgcaaccgc tctgcttctg cctgcatcgc tgcattattg gcatgagcca ggtcaataac
     4681 ctccggatga gtggaattat ggcgaagcgc ctcattcaca atatccttca gatgttgagt
     4741 cagcgcatcc cggtttgctt ttgctttagc ctgtgcttct gcggcagctt ttgctcgggc
     4801 agcctgttct gcctgcgttt tcttcaattg agcagtagac catttagctg tggcatgaat
     4861 ggctgcagaa ctttcacttt tgctacctcc agttccacca ccaccgccag agccactccc
     4921 gtctggatta ccattcaaaa gagtaatgat tacctgcccc ttatcatcat aaggaacacc
     4981 atctttatag tacgctacag ctgtttccat tataaaatcc tctttgacat taaaaacaaa
     5041 tagttaaaag aaatatactg tatatataat cactggtttt atatacagtt taaaattacg
     5101 ccgctgcatt ttccctgtca accctgtgga ttttcatttt tgtgaaaacg atc
//
LOCUS       DADLSE010000217         5148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLSE010000217 DADLSE010000000
VERSION     DADLSE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.218"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..373
                     /gene="recB"
                     /locus_tag="JN692_003911"
     CDS             <1..373
                     /gene="recB"
                     /locus_tag="JN692_003911"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="helicase/nuclease; with RecCD catalyzes the
                     exonucleolytic cleavage of DNA; RecD has 5' to 3' helicase
                     activity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7266061.1"
                     /translation="VRGMLKGFIDLVFRHEGRYYLLDYKSNWLGEDSSAYTQQAMAAA
                     MQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGVDKEHPQQGIYTTR
                     PNAGLIALMDEMFAGMTLEEA"
     gene            373..2199
                     /gene="recD"
                     /locus_tag="JN692_003912"
     CDS             373..2199
                     /gene="recD"
                     /locus_tag="JN692_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7266062.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMFEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(2261..3592)
                     /gene="argA"
                     /locus_tag="JN692_003913"
     CDS             complement(2261..3592)
                     /gene="argA"
                     /locus_tag="JN692_003913"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7266063.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            3825..5078
                     /locus_tag="JN692_003914"
     CDS             3825..5078
                     /locus_tag="JN692_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7266064.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
BASE COUNT         1209 a         1304 c         1418 g         1217 t
ORIGIN      
        1 ggtacgcggc atgttaaaag gctttatcga cctggtattc cgccacgaag ggcgttatta
       61 cctgctcgac tataaatcca actggctggg tgaagacagt tcggcttaca cccaacaggc
      121 tatggcagca gcgatgcagg cacaccgcta tgatctgcaa tatcagcttt ataccctggc
      181 gctgcatcgt tatctgcgcc atcgcattgc tgattacgac tatgatcttc actttggcgg
      241 cgttatctat ctgttcctgc gtggcgttga taaagaacat ccgcaacaag ggatttacac
      301 aacccgaccc aacgccgggt tgattgccct gatggatgag atgtttgccg gtatgaccct
      361 ggaggaggcg taatgaaatt gcaaaagcaa ttactggaag ctgtggagca caaacagcta
      421 cgcccgctgg atgtgcaatt tgccctgacc gtggcgggag atgaacatcc tgccgtcacc
      481 ctcgcggcgg cactgttaag tcatgatgcc ggagagggac acgtctgttt gccgctttca
      541 cgactggaaa ataacgaggc gtcgcatccg ctgttggcga cctgtgtcag tgaaatcggt
      601 gagctacaaa attgggaaga atgcttgctg gcttctcaag cggtcagcag gggagatgaa
      661 cccacgccga tgatcctctg tggcgatcgt ctttatttga atcgcatgtg gtgtaacgag
      721 cggacggtag cgcgcttctt caatgaggtg aatcatgcca ttgaggttga tgaagctcta
      781 ctggcgcaaa ccctggacaa actttttcca gtaagcgatg aaattaactg gcaaaaagtt
      841 gcagcggcag tggcgctgac gcggcggatc tcggtgattt ccggcggccc aggcaccggt
      901 aaaacgacca ccgtggcgaa gttgctggca gcgttaattc aaatggccga cggcgaacgc
      961 tgccgtatcc gtctggctgc accaacgggt aaagctgccg cgcgcttaac cgaatctctc
     1021 ggcaaggctt tgcgacagtt accgctgacc gatgaacaaa agaaacgcat tccggaagat
     1081 gccagcactt tgcaccgatt gctgggcgcg cagccgggta gccagcgttt acgtcatcat
     1141 gccggtaacc cgctgcatct tgatgtgctg gtggtagatg aagcgtcaat gatcgatctg
     1201 cctatgatgt cgagactgat cgacgccttg ccagatcatg cgcgagtgat ctttctcggc
     1261 gatcgtgatc aactggcctc ggttgaggct ggggctgtgc tgggcgatat ctgcgcttat
     1321 gccaacgcgg gctttaccgc cgagcgtgcc aggcagctaa gccgcctgac ggggactcac
     1381 gttccggcag gaaccggcac agaagcggca tctttgcgcg acagtctctg cctgctgcaa
     1441 aaaagctatc gtttcggcag cgattctggc attggtcagt tagctgcggc gatcaaccgt
     1501 ggtgataaaa cggcagtgaa aaccgttttt cagcaggatt ttactgatat cgaaaaacga
     1561 cttttacaga gcggcgaaga ttatattgcg atgtttgagg aagctcttgc gggttacgga
     1621 cgttatctgg atctgctgca agcgcgtgcc gagccggatt taatcattca ggcgttcaat
     1681 gagtaccagc ttttgtgcgc cctgcgggaa gggccgtttg gcgtggctgg actgaatgag
     1741 cgaattgagc agtttatgca acagaagcgc aaaattcatc gtcatccgca ctctcgttgg
     1801 tacgaaggcc gaccggtgat gattgcccgt aatgacagcg cgcttgggtt gtttaatggc
     1861 gatatcggta ttgcgctgga tcgcgggcag gggacgcgcg tctggtttgc gatgccggac
     1921 ggcaatatta agtctgtgca accgagtcgc ttgccagagc acgaaactac gtgggcgatg
     1981 acggtacata aatcgcaggg atcggagttc gaccatgcgg cgttgatttt accgagccaa
     2041 cgcacgccgg tagtaacgcg agagctggta tacaccgcgg tgacccgcgc gcgtcgtcgt
     2101 ctgtcgctgt atgccgatga gcgtatatta agtgcggcaa tcgccactcg tactgagcgg
     2161 cgcagtggtc tggcggcgtt gtttagttcg cgggaataaa cgtaattgcc ggatgcgacg
     2221 cacgagtgtt acgcctgccg catccggtga ttttcatcgc ttaccctaaa tccgccatca
     2281 acactttgga tttacgctgg tagttgtaca actgcttttt gctctcgggc agtaaatcaa
     2341 tatccaccgg ggtaaatcca cgttcctgga accagtgaat actgcgcgtg gtcagcacaa
     2401 acaatttgct taagccgctc tgcttcgcct gagcggcaat gcgttccagc agaacctcgc
     2461 cccgtgatga actgcggtaa tccgggtgaa ctgccacaca ggccatttcc ccaatcttct
     2521 cttccgggaa cggatagagc gcggcgcagg caatagtcgt gttatcgcgc tgaataatgg
     2581 tgaatttgtc gatttccatc tccagctgct cgcgagaacg gcgcaccaga ataccttgct
     2641 gctccagcgg gcgaatcaac tccagaatac cgccaatatc gttgattgtt gcgcgacgaa
     2701 tctgctcggc gctttccatc acaatctgcg taccgatacc gtcgcgtgag aacaactctt
     2761 gcaacagcgc gccatcttcc tgataactga ttaaatgaca gcggcgcacg ccgctgcggc
     2821 aggctttcac tgcgccacgc aaaaagcgca ccgtaccgga gttgtaatcg cctttctctt
     2881 cctgggcttc tacccgtgct tgcgcttcgt tagggaaaag ttcggagaca atatcaccgt
     2941 cgtcattagt aacgccctgg gaagagcaaa aaccaatcat cttttcagct ttcagtttga
     3001 tggctagttg agtggcaatc tcttccgagg tcagattaaa gctctcgcca gtgactgaaa
     3061 cagcgaccgg ccccattagc actattgcac cgctttccag ttgacgatgg atcgcgtctt
     3121 catcaatccg ccggatacgc ccgctatggc agtaatccac gccgtcatcg acgcccagcg
     3181 gctgggcaat aataaaattg ccactaacga cgttgatatg cgcgccctgc agcggcgtgt
     3241 tattgagact catcgacagg cgagcagtaa tatccagttg caatgttccc gcagcctgct
     3301 tcaccagttc cagtgttttg gcgtcggtca cacgtatatt cttgtgatac agcggttcgt
     3361 ggtgatgcgc agccagattt gcgtcgatct gcggacgtgc gccatagacc accaccagac
     3421 ggatgccgag gctgtgcaac aacccgatat cattaacgat actggagaaa ttctcatgct
     3481 caatggcttc accgccgagc atgatgacaa acgtttttcc ccggtgggta ttgatatagg
     3541 gaaccgaatg gcggaatccc tcgaccagct cggttttacg ttcctttacc acggcacacc
     3601 ccctttgcat gattattcga aattattgta tttttattct gttttttcgc agggtgcaag
     3661 tgtaaatttt atgcgagagc gactttttta tcagtaatgc cgtgaatata aaaagaatgt
     3721 ttaccgttta ttagatgaca gattatgcgt ctttcgctaa agtttccggt caaattagtc
     3781 gtttacttgt tacacagctt agatttcttt tgctcgggag aggcatgtca ggatccaaca
     3841 ctgcaatcag ccgtcgtcgt ttactgcaag gcgcgggcgc tatgtggcta ttgagcgtaa
     3901 gtcaggtcag cctggctgcg gttagccagg tcgtggcggt gcgcgtctgg cctgcgtcca
     3961 gctacacccg cgtgacggta gaatcaaatc gtcagctgaa atataagcag ttcgcgttga
     4021 gtaaccctga acgcgtggtg gtggatatcg aagatgtaaa cctgaactcg gtactcaagg
     4081 ggatggcggc acaaattcgt gcagacgacc cgttcatcaa gtcggcgcgc gtcgggcaat
     4141 ttgacccgca aaccgtacgc atggtttttg aattaaagca aaacgtaaaa ccgcagctgt
     4201 ttgcccttgc gccggtcgcc gggtttaaag agcgtctggt gatggacctc tatccggcca
     4261 atgcacagga tatgcaggac ccgctgctgg cgctgctgga ggattacaac aaaggcgacc
     4321 tcgaaaagca ggtgccgcca gcgcaaagtg gtccacaacc gggtaaagca gggcgcgatc
     4381 gtccgattgt cattatgctt gaccctggcc acggtggcga agactccggt gcggtgggga
     4441 aatacaaaac gcgcgaaaaa gatgtggtat tgcaaatagc tcgccgtttg cgctctctga
     4501 tcgagaaaga gggcaatatg aaggtgtaca tgacgcgcaa tgaagacatc ttcattccgt
     4561 tgcaagtgcg cgtagcaaaa gcccagaaac agcgtgctga cctgtttgtc tctatccatg
     4621 ccgacgcctt taccagtcgt cagccgagcg gttcctcggt gtttgcgctc tcaaccaaag
     4681 gcgcaaccag tactgcggca aaatatctgg cacaaaccca gaacgcctcg gacttgattg
     4741 gtggcgtgag caaaagcggt gaccgctatg tcgaccacac catgttcgat atggtgcagt
     4801 cgctgactat tgctgacagc cttaagtttg gtaaagcggt actgaataag ctcggaaaaa
     4861 tcaacaagct gcataaaaat caagttgaac aggccgggtt tgccgtacta aaggcaccag
     4921 atattccctc cattctggtc gaaacggcgt ttatcagtaa cgttgaggaa gagcgtaaac
     4981 tgaaaacggc gactttccag caggaagttg cggagtctat tcttgcgggg attaaagcgt
     5041 attttgccga tggggcaacg ctggcgagaa ggggatgata aaaaggcgct gaatggcgct
     5101 tttttatggg agatcagaaa caaaaaaaca cccgttaggg tgttcaat
//
LOCUS       DADLSE010000218         5144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLSE010000218 DADLSE010000000
VERSION     DADLSE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.219"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1165
                     /gene="tnaA"
                     /locus_tag="JN692_003915"
     CDS             <1..1165
                     /gene="tnaA"
                     /locus_tag="JN692_003915"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HAY7266065.1"
                     /translation="ESVKNIFGYQYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKM
                     VAFSNYFFDTTQGHSQINGCTVRNVYIKEAFDTGVRYDFKGNFDLEGLERGIEEVGPN
                     NVPYIVATITSNSAGGQPVSLANLKAMYSIAKKYDIPVVMDSARFAENAYFIKQREAE
                     YKDWTIEQITRETYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVVQE
                     GFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGHAA
                     FVDAGKLLPYIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELL
                     RLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            1256..2503
                     /gene="tnaB"
                     /locus_tag="JN692_003916"
     CDS             1256..2503
                     /gene="tnaB"
                     /locus_tag="JN692_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HAY7266066.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2635..3810
                     /gene="mdtL"
                     /locus_tag="JN692_003917"
     CDS             2635..3810
                     /gene="mdtL"
                     /locus_tag="JN692_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7266067.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALEIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3785..4744
                     /gene="yidZ"
                     /locus_tag="JN692_003918"
     CDS             3785..4744
                     /gene="yidZ"
                     /locus_tag="JN692_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7266068.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLV
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPHYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4901..>5144
                     /locus_tag="JN692_003919"
     CDS             4901..>5144
                     /locus_tag="JN692_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7266069.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFS"
BASE COUNT         1165 a         1270 c         1284 g         1425 t
ORIGIN      
        1 cgagtcagtg aaaaatatct ttggttatca atatactatt ccgactcacc agggccgtgg
       61 cgcagagcaa atctatattc cggtactgat taaaaaacgc gagcaggaaa aaggcctgga
      121 tcgcagcaaa atggtggcat tctctaacta tttctttgat accacgcagg gccatagcca
      181 gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa gccttcgata cgggcgtgcg
      241 ttacgacttt aaaggcaact ttgacctcga aggattagaa cgcggtattg aagaagttgg
      301 cccgaataac gtgccgtata tcgttgcaac catcaccagt aactctgcag gtggtcagcc
      361 ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg aagaaatacg atattccggt
      421 ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc atcaagcagc gtgaagcaga
      481 atacaaagac tggaccatcg agcagatcac ccgcgaaacc tacaaatatg ccgatatgct
      541 ggcgatgtcc gccaagaaag atgcgatggt gccgatgggc ggcttgctgt gcatgaaaga
      601 cgacagcttc tttgatgtgt acaccgagtg cagaaccctt tgcgtggtgc aggaaggctt
      661 cccgacatat ggcggcctgg aaggcggcgc gatggagcgt ctggcggtag gtctgtatga
      721 cggcatgaat ctcgactggc tggcttatcg tatcgcgcag gtacagtatc tggtcgatgg
      781 tctggaagag attggcgttg tctgccagca ggcgggcggt cacgcggcat tcgttgatgc
      841 cggtaaactg ctgccgtata tcccggcaga ccagttcccg gcacaggcgc tggcgtgcga
      901 gctgtataaa gtcgccggta tccgtgcggt agaaattggc tctttcctgt taggccgcga
      961 tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg ctgcgtttaa ccattccgcg
     1021 cgcaacatat actcaaacac atatggactt cattattgaa gcctttaaac atgtaaaaga
     1081 gaacgcggcg aatattaaag gattaacctt tacctacgaa ccaaaagtat tgcgtcactt
     1141 caccgcaaaa ctgaaagaag tttaattaat actacagagt ggctataagg atgttagcca
     1201 ctctcttacc ctacatcctc aataacaaaa atagccttcc tctaaaggtg gcatcatgac
     1261 tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt atggttatag caggtacagt
     1321 aattggtgga ggtatgtttg ctttacctgt tgatcttgcc ggtgcctggt ttttctgggg
     1381 tgcctttatc cttatcattg cctggttttc aatgcttcat tccgggttat tgttattaga
     1441 agcaaattta aattatcctg tcggctccag ttttaacacc atcaccaaag atttaatagg
     1501 taacacctgg aacattatca gcggtattac cgttgccttc gttctctata tcctcactta
     1561 tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg atatcaatga atttgggcta
     1621 tcacgctaat ccacgtattg tcgggatctg cacagccatt ttcgttgcca gcgtattgtg
     1681 gataagttcg ttagccgcca gtcgcattac ctcattgttc ctcgggctga agattatctc
     1741 ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat tactccatcc tgcgcgatgc
     1801 caccagcacc actgcgggaa cgtcttactt cccgtatatc tttatggctt tgccggtgtg
     1861 tctggcgtca tttggtttcc acggcaatat tcccagcctg attatttgct atggcaaacg
     1921 caaagataag ttaatcaaaa gcgtggtatt tggttcgctg ctggcgctgg tgatttatct
     1981 cttctggctc tattgcacga tggggaatat tccgcgcgaa agctttaagg cgataatctc
     2041 ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc ggcaccaaac agcacggcat
     2101 tatcgagttt tgcctgctgg tgttctctaa cttagctgtt gccagttcgt tctttggtgt
     2161 cacgctgggg ttgttcgatt atctggcgga cctgtttaag attgataact cccacggcgg
     2221 gcgtttcaaa accgtgctgt taaccttcct gccacctgcg ttgttgtatc tgatcttccc
     2281 gaacggcttt atttacggga tcggcggtgc cgggctgtgc gccaccatct gggcggtcat
     2341 tattcccgca gtgcttgcaa tcaaagctcg caagaagttt cccaatcaga tgttcacggt
     2401 ctggggcggc aatcttattc cggcgattgt cattctcttt ggtataaccg tgattttgtg
     2461 ctggttcggc aacgtcttta acgtgttacc taaatttggc taaatccttc aagaagccag
     2521 ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt ccaaatggca actcgcctta
     2581 tcctccttca ccacgtatgc tttgcgtcac cttactatca ggacgcttta gcccatgtcc
     2641 cgctttttga tttgtagttt tgccctggtt ttactttatc ccgccgggat tgatatgtac
     2701 ctcgttggtt taccgcgcat cgccgccgat ctcaatgcca gcgaagcgca gttgcatatt
     2761 gcgttctccg tatatctggc ggggatggca gctgcgatgc tatttgccgg taaagtggcc
     2821 gatcgttcag ggagaaagcc ggtcgccata cccggcgcgg cgctatttat tattgcctcg
     2881 gtgttctgtt cactggctga aaccagcacg ttatttcttg caggccgatt tctacagggg
     2941 ttgggcgcag gctgttgtta cgtggtggcg ttcgccattt tgcgcgacac gctggatgat
     3001 cgacgtcggg ctaaagtgct gtcattactc aacggtatta cctgcatcat tccggtgtta
     3061 gcgccagtgc tcggacatct gattatgctt aaattcccgt ggcagagtct gttctgggcg
     3121 atggcaatga tgggcatcgc ggtactgatg ttgtctttgt ttattttaaa agaaacgcgc
     3181 ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata gcgagtcgct gcttaatcgt
     3241 tttttcctca gccgtgttgt tatcaccacc ctcagcgttt cggtgatcct cactttcgtc
     3301 aatacatcgc cggtattgct gatggaaatc atggggtttg agcgcggaga atacgccacc
     3361 attatggcgc tgaccgctgg cgtcagcatg accttttcat tctcgacgcc atttgcgctg
     3421 gaaattttta agccacgtac gttgatgatc acctcgcagg tgttattcct tgcggcgggg
     3481 atcacccttg ccgtttcacc ttcccatgcg gtttctctgt ttggtatcac gctgatttgc
     3541 gccggtttct cggtaggttt tggcgtagcg atgagtcagg cgttaggacc attttcatta
     3601 cgcgcgggcg tagccagctc gaccttaggt attgcgcagg tttgcggttc gtcactgtgg
     3661 atttggctgg cagcggtggt tggtatcggc gcatggaata tgctgatcgg gattctgatt
     3721 gcctgtagca tagtgagcct gttgctgatt atgttcgtcg cgcctggacg ccccgttgcc
     3781 gctcatgaag aaatccatca ccacgcttga tctcaatctg ctgctctgtc tgcaactgct
     3841 gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg aacgtgaccc cttcagcggt
     3901 gagtaagtcg ctggtaaagt taagagcgtg gtttgacgac ccgctctttg tgaactcacc
     3961 gctggggctg tcgcccacac cgctaatggt cagcatggag caaaatctgg cggaatggat
     4021 gcaaatgagc aatctgctgc tggataaacc gcaccaccag acaccgcgcg gcctgaagtt
     4081 tgagttggcg gcggaatcgc cgctgatgat gatcatgctt aatgcgctgt cgaaacggat
     4141 ctaccaacgt tacccgcagg cgaccatcaa actacgtaac tgggattacg attccttaga
     4201 tgccattact cgtggtgaag tggatattgg tttttccggt cgcgaaagcc atcctcgctc
     4261 gcgggagctg ttaagctcgc taccgttagc cattgattat gaagtgctgt ttagtgatgt
     4321 accctgcgtc tggttacgca aagatcatcc ggcactgcat gaaacgtgga atctggacac
     4381 ctttttacgt tatccgcata tcagcatttg ctgggaacag agcgacacct gggcgctgga
     4441 caatgtgtta caggagctgg ggcgcgaacg cacgattgct atgagcctgc cggaattcga
     4501 gcagtcactg tttatggcag cgcaacccga caatctgcta ctggcgaccg cgccgcacta
     4561 ctgtcagtac tacaatcaac tccatcaact gccgttggtt gctcttcctc tcccgtttga
     4621 cgaaagccag caaaaaaagc tggaagttcc ttttaccctg ctgtggcata aacggaacag
     4681 ccataatccg aagatcgtct ggttacggga aaccattaaa aacctttacg cgtcgatggc
     4741 ataaccgaat cgtatgaaac gcgacccaat ttcacaataa aatgtaaaaa agttgtaata
     4801 agcttgtctg aatcgaactt ttagccgctt tagtctgtcc atcattccag taaatgatta
     4861 ctcttgtatt cataatggac cattaagcat ggagcgaaaa atggcgactc actttgcccg
     4921 agggatttta acggaaggac atctgatttc tgttcgtctc ccctcccagt gtcatcaaga
     4981 agcccgaaac attccccctc atcgtcaaag ccgttttctg gcgtccagag gtttactcgc
     5041 agaactgatg ttcatgctgt atggcattgg cgaattgccg gaaatcgtca ccctgccgaa
     5101 aggtaaaccg gttttcagtg ataaaaattt gccttcgttt tcta
//
LOCUS       DADLSE010000219         5065 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLSE010000219 DADLSE010000000
VERSION     DADLSE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.220"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(106..181)
                     /locus_tag="JN692_003920"
     tRNA            complement(106..181)
                     /locus_tag="JN692_003920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Thr,seq:tgt)
     gene            complement(187..261)
                     /locus_tag="JN692_003921"
     tRNA            complement(187..261)
                     /locus_tag="JN692_003921"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Asn,seq:gtt)
     gene            complement(522..1145)
                     /locus_tag="JN692_003922"
     CDS             complement(522..1145)
                     /locus_tag="JN692_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7266070.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(1142..1330)
                     /locus_tag="JN692_003923"
     CDS             complement(1142..1330)
                     /locus_tag="JN692_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7266071.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(1327..1689)
                     /locus_tag="JN692_003924"
     CDS             complement(1327..1689)
                     /locus_tag="JN692_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7266072.1"
                     /translation="MNEYQFVLPYPPSLNTYWRRRGSRYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKARLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(1686..1976)
                     /locus_tag="JN692_003925"
     CDS             complement(1686..1976)
                     /locus_tag="JN692_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7266073.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKP"
     gene            complement(1976..2746)
                     /locus_tag="JN692_003926"
     CDS             complement(1976..2746)
                     /locus_tag="JN692_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7266074.1"
                     /translation="MNAVIIANAEIVPIEFNGERVVTYQMIADIHGVPVATIQNGYAR
                     NSEYFIDREDTYFIDFHENTFRRGFEIPPRGMRVFTESGYLMLVKTLRDETAWRVQRE
                     LVRNYFRGQKEDKSALPDFTNPAEAARAWANEYEQKVKLEQQLAIAAPKVEFADRVGE
                     ASGILIGNFAKVVGIGPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVN
                     TNHGIQISFTTKITGRGQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(2761..2997)
                     /locus_tag="JN692_003927"
                     /pseudo
     CDS             complement(2761..2997)
                     /locus_tag="JN692_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3072..3752)
                     /locus_tag="JN692_003928"
     CDS             complement(3072..3752)
                     /locus_tag="JN692_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7266075.1"
                     /translation="MATQISVETLSPITHNQIPVITTELLAQLYCTEINNIKVNYTRN
                     SERFVEGKHFFKVVGDELKNLRVTLSNSQTPVSPKTRSLILWTERGAARHAKMLETDQ
                     AWDVFEKLEDCYFSQTSKSAGHHEKKTSELSAKEANSLVWLWDYAHRSQALFRELYPA
                     LRQIQSNYSGRCHDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSAWVRLKNKE
                     LPPSLHHY"
     gene            4074..4403
                     /locus_tag="JN692_003929"
     CDS             4074..4403
                     /locus_tag="JN692_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7266076.1"
                     /translation="MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4393..4701
                     /locus_tag="JN692_003930"
     CDS             4393..4701
                     /locus_tag="JN692_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7266077.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4743..4919)
                     /locus_tag="JN692_003931"
     CDS             complement(4743..4919)
                     /locus_tag="JN692_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7266078.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4916..>5065)
                     /locus_tag="JN692_003932"
     CDS             complement(4916..>5065)
                     /locus_tag="JN692_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7266079.1"
                     /translation="DVLPGLEGECVNIRESSAQMSVKRMASLIEYTTAWAIGQGVRFT
                     DRRYE"
BASE COUNT         1216 a         1265 c         1197 g         1387 t
ORIGIN      
        1 cgcaaataac ggatggcgta gttcttatat tgggaagggg aaaagaaggc cgcagcgtaa
       61 ctgtcactga tgaattcagg atagccagtg gctacggctc agttatggtg ctggttaacg
      121 gacttgaacc gctacccatt cgcttacaag gcgactgctc taccattaga gctaaaccag
      181 catatttggc gggacagcgt ggactcgaac cacgataaga aggttaacag ccttccgtaa
      241 tgacctttat acgactgacc caaataaaaa atcccgaaag caggctctat tcctatgtgc
      301 tcccgagttt atgatgcgca tgtcagtgcg cttagcgaaa aacgccgtaa accctcgccc
      361 gacagggcgg ggagcagtca caacttaaag atgaggaggg taaaaaaccg aaagaatgtg
      421 aacggatata aacctgccat tcttgagtca aatttaccca actttattca aaaagtcaat
      481 atcatgccgt taatatgttg ccatccgtgg caatcatgct gctaacgtgt gaccgcattc
      541 aaaatgttgt ctgcgattga ctcttctttg tggcattgca ccaccagggc gtcatacagc
      601 ggcttaacag tgcgtgacca ggtgggttga gtaaggtttg ggattagcat cgttacagcg
      661 cgatatgctg cgcttgctgg catccttgaa taaccgacgc ctttacatct tccgcactct
      721 ttctcaacaa ctctccccca ctgctctgtt tttgctatat caactgcccg gcctgtaccg
      781 tggcaatctc tgcatcttgc gcccggcgtc gcggcactac gacaataatc cgcataagcg
      841 aatgttgcga gcacttgcag tacctttgcc ttagtgtttc cttcgagctt tgccacacca
      901 cggtatttcc ccgatacctt gtgtgcaaat tgcatcagat agttgatagc cttttgtttg
      961 tcgttctggc tgagttcatg cttaccgcaa aatgcagcca ttccgaatcc ggcttgtgat
     1021 tgcgccatcc ccatagcagc catcacatca gtaccggaaa gagagtcaga agccgtagcc
     1081 cgtggtgagt cgctcatcat cgggcttttt ggcgaatgaa atttagctac gctttcgagt
     1141 ctcatcgtct tcccctcttg ccctgtttga ccatcaggac gccgttaact attacgtgac
     1201 gctcgccttt gctgtctcgg ttgtacttga gcactgttcc tcttgcgcag gaaagcatcc
     1261 tcgccacttc ggtctgattg cctcgtgtct ggataagaag ctctggtatc gtttgaattg
     1321 tggcgttcat acgttctcca gttcggtgat ttttattcca agccttccgc ctggtacttt
     1381 cacaccacga attacgcgaa tgtcatcgaa ttgctcgtcg tcttccgcaa atccggcgtg
     1441 gataagagag tcgagtagcc ccttcaggat gttatcgagg tcgcggcggc gggagtctgg
     1501 aacgtctgcg atgactttga tgcggagtcg tgctttggtg aaaatatcta acttgagttg
     1561 gcggatgatt tgctgaacgt cttttcggta tttctggcct ttatcgctga tgtagtatcg
     1621 gcttccccgt cttcgccagt aggtgttcag cgacggcggg tatggaagca caaactgata
     1681 ttcgttcatg gcttaatctt cccctccttc agcagtatcg cctgcgtcct gatcacgcct
     1741 tcgaggtggt aaagcctggc gtctttgttg tcgagattat gggtgcgtcg gtcgatttca
     1801 tcgtgacacg cgctacaagc ccatgcgccg atcaggtcgt caggcttcat tcccgttccg
     1861 caaattccag ccatccggta atgtgccaga actgtagttt cagggttgcc attgcatacg
     1921 ccgtaaatac gtacctggca ttctctgccg cgtgcttctt tgcgtaggtt agccattaag
     1981 cagcctcccc ggttactttc agcattccgt tatcgagcag ctttctggtc agccactgtt
     2041 gaccacgccc ggtgattttt gtggtgaacg atatctgtat tccgtgattt gtgttgaccg
     2101 ctgtttcttt cactgtgaaa tagccgcgat ccatatattc ctgcattggc acattgcgcc
     2161 gggaacctga agcaataagg attttgtgat cacgcatcca cgcaaacagt ttgtttgggc
     2221 caataccaac aacctttgca aagtttccaa tcaaaattcc gctggcctcg ccaacgcgat
     2281 cggcaaactc aactttaggt gcggcaatcg cgagttgttg ctccagcttc actttctgct
     2341 catattcgtt tgcccacgct ctggcggctt cagcagggtt tgtgaagtcg ggcaaggctg
     2401 acttatcttc tttttggcca cgaaaataat tgcgaacaag ctcacgttgc actctccacg
     2461 ccgtctcgtc gcgcagggtt ttaaccagca tcaaatagcc tgattcagtg aaaactcgca
     2521 tgccacgagg aggaatttca aaaccccttc taaaggtatt ttcatgaaaa tcaataaaat
     2581 atgtatcttc cctgtctatg aaatactcag agtttcgcgc atagccattt tgaatagtgg
     2641 ctactggaac cccgtgaata tcagcaatca tttgatacgt aacaacacgc tcgccattaa
     2701 actcaatcgg cacgatttct gcgttggcaa taattacggc attcatgcaa cacctccttc
     2761 aacgacaata atgctgtcgc gcttaccttg ttcgccttcg aagacgtaat gctcgtactg
     2821 aacattgaac cctaagttat tgattctttc ggaaacctca aattgaggat gccggataac
     2881 accattttta gccagcgttt cgatggtacg tttcacattg tcatgacgct tacccaccaa
     2941 ctcagcgatt tcaatgctgg tcattttgat ggcgttgcta tttatcagct cgttcattgt
     3001 catgtcctct catattgaaa attcagcaat aaaaaaccca gccgaagctg ggtcgttgcg
     3061 ttggcaatct gtcagtagtg atgtagtgaa ggaggtaatt ctttgttctt aagtcttacc
     3121 catgcagaaa gattcgttgg tccgtctggc tcattaatat caacatctcg tgtgtgattg
     3181 attaaaacgt ctctcgccat tccgataaca tacgagaact catgaccgta gtcgtggcat
     3241 ctgccggaat agttagactg aatttgtctt agcgccggat acagttcgcg gaataatgcc
     3301 tgtgagcggt gggcataatc ccacaaccat acaaggctgt ttgcttcttt tgcagaaagc
     3361 tcgctggttt tcttctcgtg atgacctgcg cttttcgaag tctggctgaa atagcagtct
     3421 tccagttttt cgaacacgtc ccatgcctga tcggtttcga gcatttttgc gtggcgggct
     3481 gctccgcgtt ctgtccagag gatgagggag cgggttttgg gggaaacagg cgtttgtgag
     3541 ttacttaaag taacccgcaa atttttcaac tcatcaccaa caaccttgaa aaagtgttta
     3601 ccctcaacaa aacgctcgga attacgggtg taatttactt tgatgttgtt gatctcggta
     3661 cagtaaagct gtgccagtaa ctcggtagtg ataacgggaa tttggttgtg tgtaattggg
     3721 gaaagagttt cgacagaaat ctgagtggcc ataatgacgc cctccggtga ttgttttgtt
     3781 tatcaccacc gccgacgcca atcggattgg gtggtgagac gtacagggtt ggcgtaaccg
     3841 gatcaccgac cggcgagcct ttcggctccc ccatacgccc caccataatt cagatgcgcg
     3901 tatacaaacg acaataaaaa acacgctcgc ggcgtgtctc tgtcgcggtg aaattccggg
     3961 acgccaatcc cgacgccaga ttttgctggc gtatgaggaa tatagccccg gatatgtgtt
     4021 gtcgtcaact acaggccttg aatgcatata acgattttgt tatgttgata cctatgaact
     4081 acactatcga atactacagt gaagatgtaa ggctggaagt cgatcggctt ccattgagta
     4141 tgcgtgcccg ataccaacat ctcgttgaac gtatgaagat atatggcagc aatctcggag
     4201 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4261 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4321 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4381 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4441 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4501 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4561 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4621 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4681 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4741 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4801 aaggttttta cgtcagattc ttttgggatt ggcttgggtt tatttctgga gcgtttcgtt
     4861 ggaaggtatt tgcagttttc gcagatgatg tcggtgatac ttcgtcgctg tcgtctcatt
     4921 cgtacctcct gtcggtaaat ctgacaccct gaccaatagc ccatgctgtt gtgtactcga
     4981 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5041 ctccttcaag gccgggcaaa acatc
//
LOCUS       DADLSE010000220         5037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLSE010000220 DADLSE010000000
VERSION     DADLSE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.221"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(44..420)
                     /gene="rnpB"
                     /locus_tag="JN692_003933"
     ncRNA           complement(44..420)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN692_003933"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(453..1598)
                     /gene="garK"
                     /locus_tag="JN692_003934"
     CDS             complement(453..1598)
                     /gene="garK"
                     /locus_tag="JN692_003934"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7266080.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1695..2585)
                     /gene="garR"
                     /locus_tag="JN692_003935"
     CDS             complement(1695..2585)
                     /gene="garR"
                     /locus_tag="JN692_003935"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7266081.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADCNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2615..3385)
                     /gene="garL"
                     /locus_tag="JN692_003936"
     CDS             complement(2615..3385)
                     /gene="garL"
                     /locus_tag="JN692_003936"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7266082.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADVRRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3401..4735)
                     /locus_tag="JN692_003937"
     CDS             complement(3401..4735)
                     /locus_tag="JN692_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266083.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1221 a         1429 c         1194 g         1193 t
ORIGIN      
        1 ctccaaaagc aaaaacccgc cgaagcgggt ttttacgtaa aacaggtgaa actgaccgat
       61 aagccgggtt ctgtcgtgga cagtcattca tctaggccag caatcgctca ctggctcaag
      121 cagcctaccc gggttcagta cgggccgtac cttatgaacc cctatttggc cttgctccgg
      181 gtggagttta ccgtgccacg gactgttacc agccgcgcgg tgcgctctta ccgcaccctt
      241 tcacccttac ctgatcccgc ttgcacgggc catcggcggt ttgctctctg ttgcactggt
      301 cgtgggtttc ccccccaggc gttacctggc accctgccct atggagcccg gactttcctc
      361 ccctccgccc gtctcccccg aagaggacga cgacgaagcg gcgactgtct ggtcagcttc
      421 ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc
      481 agtgtcgcgg caatattacg tgaagcacgg cagatattgt cataagcccc gcggaatgct
      541 tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg catcaatgcc atgctgatgt
      601 acaacgccaa catcatcggt caggctaccc gcaatgccaa tcaccggttt atggtacttc
      661 ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa tactctggct gtcaatacgc
      721 ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt cctccagatt cagcgccgta
      781 gtgacgattt caataccact tttcagttcc gcaccaagaa acgccatcag cgccgcaccc
      841 ataccgcccg cagctcctgc gccggggaca tcttttacat caacatgcag cgctttttta
      901 atgacatcgg catagtgaga gaggttattg tccagctcaa caatcatcgc ttcactggct
      961 cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc ccaccagcgg attggtgaca
     1021 tcacaagcga cgcgaatgac gcaatctttt aagcgcggat cgaggccgga aatatcaata
     1081 tcattcagag tattaagact accgccgcca aaaccaattt cattgccgtt ggcgtcgcat
     1141 aatttcgccc ccagcgcctg taccatgcct gcgccgccat catttgtagc gctaccgcca
     1201 atgccgataa taatgtttgt cgcgccgctc tccagcgcct gcaggattaa ctcgcctgtg
     1261 ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag gtaccagctc cagcccactg
     1321 gccgccgcca tttcaataaa cgcggttttg ccatcgccgg agatccccca actggcattc
     1381 actttctcgc ccagcggccc tgtaacccag gcgtgacgtt cagaaccctg ggtggctgca
     1441 atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg gaatagaaac gtactgtgca
     1501 tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg caacctcgct ggcagataaa
     1561 ctttctttat aagagtctgg ggcgattacg attttcatac ctatgcctgt taccacatga
     1621 cgccggaggg cgtttctctt attcggcctg gattccaggc ccggattgca atacgccatc
     1681 cgggcacgac gtcattaacg agtaacttcg actttcgcca gtttttcgta gtagcacgcc
     1741 agggcgctat gatccgccgt tcctaaacca tctgctcgca gtgcctgcat catctccata
     1801 accgcagctg tgagcggcag ttgcgcgccg acgccgtggg aagtatccag cgcatttgcc
     1861 agatccttaa tatgcagatc aatacggaag cccggcttga agttgcggtc catcaccatc
     1921 ggcgctttgg catccagcac ggtactgccc gccagtccac cgcgaattgc ctgatacacc
     1981 aggtccgggt taacgcccgc tttagttgcc agcgttaacg cttctgacat cgcggcaata
     2041 ttcagcgcca caatgacctg attcgccagt ttggtgacgt tacctgcacc aattgccccg
     2101 gtatgcacca cggaacccgc cattgctttc atcaaatcat agtatttgtc gaaaatagcc
     2161 ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg ctttcggttc accgccgctc
     2221 accggagcat ccagcatatc aatgcctttc gctttcagcg cttcgctgat ttcacggctt
     2281 gccagcggtg cgatagaact catatcgatc aataccgtac ctggcttcgc gccttcaata
     2341 atgccattct cacccagcgc cacctctttc acatgagggg agtttggcag catggttatg
     2401 atgacgtcgc actgttcagc gatcgcttta gccgtagagg ctgtttctgc acctgcagca
     2461 atcacatcag caatagcttc tgggttacag tcagcaacca ccagcgagta acctgctttc
     2521 agaaggtttt tactcattgg tttacccata atccccaggc caataaaacc aactttcata
     2581 gtcatatcaa tcatctctct tgttgcggtg gtggttattt tttaaaggta tcagccagtt
     2641 tatgagtggc agagcggaag acgccgagat cgctgccgac agccacaaac gtcgcgcccc
     2701 attccagata acgacgcaca tcggcttcga ccggcgcgag gataccgctg ggtttgccgt
     2761 gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg tacatccggg tgtgatgcat
     2821 tgccgagatg gcctaatgcc gcggccagat cgctggggcc gacgaagatg ccgtctacgc
     2881 cttcggtagc ggcaatggca tcgacgttat ctacgccctg ctgactttct atctggacca
     2941 gaatagtgat gttcttgttc gactgagcga aataatccgc cacggtgcca aacatattgg
     3001 cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg gtaacgggtt gatgccaccg
     3061 cctgctctgc ttcctctttt gtttctacaa aaggaatcag gaagttatag aaaccgatat
     3121 ccagaagacg cttaataatt accggctcgt tagtcggcac tcgcactact ggcgcgctgg
     3181 cgctgccttt caaggccatt aactgcggaa taaacgtgga gatatcgttt ggcgcatgtt
     3241 cgccatccag caccagccag tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt
     3301 tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc agccagtgcg gctttgaatt
     3361 tattcgggaa aacatcgtta ttcatcgctt atacctttgc ttatttctgc aattccatac
     3421 gtttaatgtc gccaactacg aagaggtagc agaccatcgc catcagcgct gaacatccca
     3481 cgaaaaccag tgctgcattg aaggagtgca gttcacttac caggtagcca atcaccagtg
     3541 gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac gccgccacag aggccaacaa
     3601 tctctttcgg cgcggtgtca gaaatcaccg gccagcccag agcaccaaat cctttgccaa
     3661 aaaaagccag cgccatcagc atgaccacca gcgtggtgtt gttggtgtag ttacataaga
     3721 tgatggtgga agccagcaac attcccagca caatcggtag cttacgtgcc agggtcaggg
     3781 ataaaccgcg tttgatcaga taatccgaga agacacctcc cagcacgccg cccgcaaaac
     3841 cacacagtgc cggaatcgag gcgaccagac ccactttcag aatcgacatg cctttttcct
     3901 gcaccagata aatcgggaac caggtgagga agaaccaggt gatggtgttg ataaaatatt
     3961 gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa ttgcttgatg taatgcagtt
     4021 tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc catatcgacc accgcgccat
     4081 tttcagagat aaacttcagc tcttcctcag acatacgtgg gtgatctgtc gggttatgaa
     4141 tcaacttgat ccacagcgcc gtcagcacaa aaccaatcac ccccataacg gtaaagacgt
     4201 gctcccatcc ccaggcgaaa gtcagccagc caagcagagg cgaaaagagc gccagcgaga
     4261 aatattgtgc cgagttaaag atggcggagg cagtaccacg ttctttcgtc gggaaccagg
     4321 cggcgacaat acgggcgttc gccgggaatg atggagcttc cgagaatccc agcataaagc
     4381 gcataaagaa catggagatc cctgcccagg ccagcgggaa catatcaaca aagccttgca
     4441 ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta aacttttttc gagccaaact
     4501 tatcaagcaa ccagccgccg gggatttgca tcagcaagta agcccagcca aaagcggaga
     4561 agatgtaacc catcgaaacc gcacttaact gcaactcttt tgccacttcg gtaccagcaa
     4621 tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac aataaaaata atcagtaata
     4681 ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac ggtgtccaga atcattttat
     4741 ttacctcggg tacttatgct gatttttatt attatgggga aggtgttatt tataagtttc
     4801 atttatgccg taacgacaat gaactcggga attagtataa gcagcgcgag aataataatc
     4861 attgtgcaaa tgctaattta attaatactt tttaaatatt attttgagca tatgcacata
     4921 aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggcggcact gcaatccctg
     4981 aaatgattga cattgatcac atttctgcgt ttaaactcct gacattctta tttcacc
//
LOCUS       DADLSE010000221         5036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLSE010000221 DADLSE010000000
VERSION     DADLSE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.222"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..207)
                     /locus_tag="JN692_003938"
     CDS             complement(<1..207)
                     /locus_tag="JN692_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HAY7266084.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAAFSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKP"
     gene            complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN692_003939"
     CDS             complement(316..729)
                     /gene="hiuH"
                     /locus_tag="JN692_003939"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7266085.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            862..1533
                     /gene="hprR"
                     /locus_tag="JN692_003940"
     CDS             862..1533
                     /gene="hprR"
                     /locus_tag="JN692_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7266086.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            1533..2891
                     /locus_tag="JN692_003941"
     CDS             1533..2891
                     /locus_tag="JN692_003941"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7266087.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN692_003942"
     CDS             complement(2999..3850)
                     /gene="hchA"
                     /locus_tag="JN692_003942"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7266088.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(4444..4653)
                     /locus_tag="JN692_003943"
     CDS             complement(4444..4653)
                     /locus_tag="JN692_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266089.1"
                     /translation="MSGDSGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLL
                     DKSDYVFAHGLNTDDQLAVGIVYQF"
     gene            4717..>5036
                     /locus_tag="JN692_003944"
     CDS             4717..>5036
                     /locus_tag="JN692_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266090.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELADAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRA"
BASE COUNT         1419 a          996 c         1190 g         1431 t
ORIGIN      
        1 aggcttgctg aactccagcg gttttccggc gggggcgggc ggacgatcgt tccctttaaa
       61 ccagctaagc agatcggcat gcgcagcgtg aggcaaagaa aatgcagctg cgctgatgcc
      121 cagtgctttt aacacctgcc gacgcttcat aaagaatacc gactcggccg taacatctga
      181 ttcttttaaa aattgattct ttttcataag ccatcacact tttttgtcgt taaccaacaa
      241 gagctgttta ctgttaatct gtcagggagt cacctcccta acagtagcag agatcagctt
      301 ttacatattt acgcattaac tgccacgata ggttgaatac ccatattggc tgagtaacaa
      361 aggcacatga taatgctcgt tcactttatt aatatgaaat tcaacgggaa tctcagggaa
      421 gaaactttca agattttgtt tcttgaaata gtccccggtt ttaaatacga cacggtaatc
      481 gccagtagtc gcagtttgct cgggccacag tgccttaatt cgtccatcct tatctgtttt
      541 ggcggtatta agttgtaacc agccgttatc cgctttcttt tcaagagtga ctgtcacgtc
      601 ggcagcgggt tttcctgttt gctggttcaa aatgtgcacg ctaagaatgt tttgttgtgc
      661 ggcataaacc aaagaaggta atgaaaatgc tgccgttgct accgagagta ctaaataacg
      721 ctttaacatg tttatatcct tgtcatgtga atgagttttg aggatagcga aaaaacgtcc
      781 agggtaacct tacagcagca ttacaatttt gtaatgaacc aggttgtttc tataacatat
      841 gatttatggc atattatttt catgaagatt ctacttattg aagataatca aaggacccag
      901 gaatgggtaa cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc
      961 agagatgggc tttatcttgc gctgaaggat gattatgcat tgatcattct ggatattatg
     1021 cttccgggta tggatggctg gcagatctta caaacgttaa gaacggcaaa gcaaacccct
     1081 gttatttgcc ttactgcaag ggattctgtc gatgacagag tcagagggtt ggacagtggg
     1141 gcaaatgatt atctggtaaa acctttttca ttttctgagt tgctagcaag ggttcgggca
     1201 caattaaggc aacatcacgc tctgaattca acattagaaa tcagtggctt aaaaatggac
     1261 tctgtcagtc aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt
     1321 cagttacttt ggctactggc ctccagagct ggcgagatta tacccagaac ggttattgcg
     1381 agtgaaattt ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc
     1441 aggctccgcg caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg
     1501 atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag attatctata accgtccgtt
     1561 taaccttgct ttttatattt ctgcagtctg ttgctggcgc tggaattgta tggactctct
     1621 ataatggcct ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag
     1681 cgcagatcaa gcagttgtta attgatgggg taaatccaga tacgttacct gtgtacttta
     1741 accggatgat ggatgttagt caggatattt tgatcattca tagtgatggc atcaataaaa
     1801 ttgttaaccg gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa
     1861 tcagcgcagc aggcatttac agaagcatta ttaatgatac agagatagat gctttacgaa
     1921 ttaacattga tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcca
     1981 gacataatat gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccatta
     2041 tactgtgctc agtattaagt ccgttgttaa tcaggacggg gttgcgagag atcaaaaagt
     2101 tgagtggcgt aacggaagcg ctgaattata acgatagccg agtgccggtt gaggttaacg
     2161 cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat catgctttag
     2221 tcaaagattt tgagcgcctt agtcaatttg ctgacgatct cgctcatgaa cttagaacgc
     2281 caattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg
     2341 aatatcaaaa aacaattgcc ggaaacattg aagagctgga aaatatttcg cggttaacag
     2401 agaatatact gtttcttgcc agggcagaca aaaacaatgt tttggtgaaa ctggattcgc
     2461 tttctctcaa taaggaagtt gaaaatcttt tggactatct tgaatatctt tcagacgaga
     2521 aagagatttg ctttaaggtc aagtgcaatc aacaaatctt tgcggataaa attttactgc
     2581 aacgaatgtt atcgaatctt attgttaacg ccattcgata ttcaccagaa aaatcgcgta
     2641 ttcatataac cagttttctt gatgccaacg gctctcttaa tattgatatc gccagtcctg
     2701 gaacgaaaat taatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc
     2761 gtcattccgt aggtcaggga cttggccttt ctttagtcaa agcgattgcc gaattacatg
     2821 gaggaagtgc tacgtatcac tatctcagta agcataacgt gttccgaatt acgttaccgc
     2881 aaagaaatta acatcgggct tcagtacgcc ataagttaac cgtgaaaaaa cgcagccacg
     2941 tcgtgagtat taacgtggct gcgattgatt atgcgcttac atcacaatgt aacagggatt
     3001 aactcgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcaa
     3061 aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat
     3121 taatgatgtt catgcccatt ttcttcaact cttcgccgaa gtaccaggtg agatgacccg
     3181 gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc tgggaatgcg caaatagaat
     3241 aaccatttag tgggttatcg ctgtggcgaa gtgccagaaa agccgccgga ccgtggcaaa
     3301 gggagataac aaaacggtca tttttgattg cccactgtaa agcggcagcc acgtcctggc
     3361 tttctggtaa accaataagt gcgccatgcc caccaggaac aaagattgct gcatattcgc
     3421 tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattacgg aataacgatt
     3481 tatgctgttc aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa
     3541 acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat
     3601 gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat
     3661 cggtcggcaa ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgatacg
     3721 gttttggata gtccacgcca tcaagatcag agacgggact cgtatattgg ctaagcgaat
     3781 attctgaagg gaagaacgca ttatcttcag caatatcgac ctgcgggttt ttacttgttt
     3841 gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact
     3901 gagctggtcg ctatttttaa ccaggctgaa aaattgagtc aatatatcga aagcggtagt
     3961 tggcgggcat aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa
     4021 ctttcctcat tgagcaatgt cgtcgcaagt ggcctcaatg attttagacc attatcagag
     4081 gtccggagcg gcgggaagag atttattgct gtggaataat tgtggtactg atgattattt
     4141 atatcccgga ttggtaggga aaagcttttt tcatgcacta tttttgcaaa tatttctata
     4201 tgggaagcgg ttttgttaca gattaaattt ataaagaaat gcatttgggg tgttgggttg
     4261 aagagtagct ggtagatgtt attgtgccag caggggcagg ggaatgtgcc gtatcgatat
     4321 gctttggcaa ttgcgtgtgc cgcagatgga gatgaaatgc caataaaaaa gcctgagttc
     4381 gatgagggac gaactcaggc tggtgttatg ttttacagta acacgcctcg gcgcgttggc
     4441 tgattagaac tggtagacga taccaactgc cagttggtcg tcggtgttca gtccgtgagc
     4501 gaaaacataa tcactcttat caagcaggtt gatctgataa gcggtatata tgttcatatt
     4561 cttattgaag taataccagg taccgatttc aatatagttc agacgatcag aatctttgta
     4621 acctgtagac tggccccctg aatctccaga caaccaatat cacttattta agtgatagtc
     4681 ttaatactag tttttagact agtcattgga gagcagatga ttgatgtctt aggaccggag
     4741 aaacgcagac ggcgtactac acaggaaaag atcgctattg ttcagcagag ttttgaaccg
     4801 ggaatgacgg tctcccttgt tgcccggcaa cacggcgtgg cagccagcca gttatttctc
     4861 tggcgcaagc aataccagga gggaagtctt actgctgtgg ccgcaggaga acaggtcgtt
     4921 cctgcctctg aacttgctga tgccatgaag cagattaaag aactccagcg cctgctcggc
     4981 aaaaaaacga tggaaaatga actccttaaa gaagccgttg aatatgggcg tgcaaa
//
LOCUS       DADLSE010000222         4901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLSE010000222 DADLSE010000000
VERSION     DADLSE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.223"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            524..1087
                     /locus_tag="JN692_003945"
     CDS             524..1087
                     /locus_tag="JN692_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7266091.1"
                     /translation="MKVSKIALAMGLSMALVSGFANAADSGTTPPANTAAEGGHGHGT
                     VHFKGEIIDAPCSISPESADQTVDLGQVANSALKDKGTSSPVAFNIKLQNCDISTYKT
                     VTATWSGTPDVNMPEAWGITGTASGAAIVLRDASEKEITLGQDTAPTTLTADDTTIAM
                     SAFLKGDGEAIVPGAFTGAADFVLSYQ"
     gene            1163..1684
                     /locus_tag="JN692_003946"
     CDS             1163..1684
                     /locus_tag="JN692_003946"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7266092.1"
                     /translation="MLRIVFLLLLLVSSTGRAVSHGHGHVQMQGTIIDTACAIATGDV
                     EQGIDFGALTASELINNGRGPSLPFTVHLVNCVLDGTDIRGLNHWKDVRITFVGDADS
                     ENHFALQGDGRGAALVIADSQGVEAKPGEPMPSTPITPGSMALHYQLWLTGGKTIRPG
                     HIHTTVRYFMEYD"
     gene            1684..4281
                     /locus_tag="JN692_003947"
     CDS             1684..4281
                     /locus_tag="JN692_003947"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025319.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7266093.1"
                     /translation="MFNQKSGNNGGLNNVRLNSLTWMLLLVLGGIPAAQSQDNMSTPA
                     NSNIPDEVVEQPNITEFNTDVMDVNDRNNIDVSHFSRAGYVMPGEYPLILVVNGTEIR
                     EVTVSFAPPEDDPQGSSPCLTPEMVKLFGLKDEATKKLIWKGNGDDRCLDEKSLPGMT
                     ATGDLSTGSLKISLPQAWLEYSAPDWDPPSRGDNGVADLLFDYNLNASKSHALSEGGS
                     SATSLSGSGTAGANFGPWRLRADWQASRYSDRGYTSQSMDWSRFYLYRALPKLGARLT
                     LGESDLDSSLFDSFRFIGGSLVTDDNMLPPNLQGYAPEVTGVARSNAKVVISQQGRVI
                     YESQVAAGPFRIQELSSSVSGTLDVRVEEQDGRVQHFQVDTASIPYLTRPGLVRYKAS
                     LGKPTDWKHHVQDVSFATGEFSWGMTNGWSLFGGLVAAKKYAALAVGIGRDLLSFGAV
                     SFDVTESKATLDTGEKKMGGSYRLSYSKRFEKIDGQITFAGYRFSEKDFMSMNDFLET
                     EYDAGSRSDTAWGRQKELYTITVSKAFPDWNMNLYGNYSHRTYWNRPDADRYSLSLSK
                     YMDVGRWKGISLNLTGYRSEAWGVKDDGVYASLSIPWSDGGSLSFNGQSTTAGTTQMV
                     GWYDRIDSNNNYSLQAGTSVGNKASASGYVSHNGDLTNGTLSASYYEGNNTSLGLSLQ
                     GGMTVTRYGAALHPGAMNGGTRLMIDSGDVAGVPLSHSGVLQRTNAFGVGVIGNLNSY
                     WRNSVGVDVNALDDDMEVSHPLTELTMTESAIGYRKIDVLEGRKLMAVVKFADGSSPA
                     FGATVWRGSHQTGVVDDNGSVWLAGVKPGVTMEVREDENPVCQVGIPERLPPENTDQS
                     LLLTCQQKS"
     gene            4395..>4901
                     /locus_tag="JN692_003948"
     CDS             4395..>4901
                     /locus_tag="JN692_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7266094.1"
                     /translation="MNRNKSIKSQPFLKSLLVMALVSQSAFQVADAAVALDRTRVIFE
                     GGNKSMSLNIRNDNTKLPYLAQSWVEDEQGNKIQSPLMALPPLQRMEPGSKSLVKIQL
                     APGVEAKLPQDRESLFYFNLREIPPKSNKANVLQLALQTRVKMFYRPKALAKNQAMNE
                     TPWQEQLTL"
BASE COUNT         1292 a         1019 c         1301 g         1289 t
ORIGIN      
        1 ggacggtgaa agcaagaagc tattaggcgc tataggtctt ttgacggttt ttgaatttcc
       61 cctcctgata tctctgttat atttttgtga atttctccaa tccaaatcaa agtcccaata
      121 agtcatggat gcatggttaa aataactatc atttgtgtaa cctttggtgc ttataacttt
      181 tctttattct tttttcagga ttacctggag ggattgacca aaaaaacatt cattgaatga
      241 tatttttcta attaatgagg ttttctaagc tcgttgaaat aaaaaatata ttttaatcat
      301 gggtttgcat gcgtttcttt cgcctttttt acacaagttg acatcgaact cagcaactat
      361 aagaatagcc acatcaccag acggtgaccg ataaaggaaa taccgtttcc atatttaaat
      421 gaattggaaa atatcaccga tttgaagtcg actcgttcgg gtaattatta atactgaatc
      481 tttaatgctt atttcttaag cttgttaaca acgggaatga ataatgaaag tgtccaaaat
      541 tgcactggca atgggtctta gcatggcatt ggtaagcggc ttcgcaaacg ctgctgactc
      601 tggtacaact cctcctgcta atactgcggc agaaggcggc catggtcacg gcactgttca
      661 ctttaaaggt gaaatcattg atgcgccatg ctctatttct ccggaatctg cagaccagac
      721 ggttgatctt ggtcaggtcg ctaactctgc cctgaaagac aaaggtacgt cttctccggt
      781 cgctttcaac attaaactgc aaaactgcga catcagcact tacaaaactg taactgcaac
      841 ctggagtggt acacctgacg ttaacatgcc ggaggcatgg ggtatcactg gtacggcgag
      901 cggtgcagct attgttctgc gcgatgcgtc cgaaaaagag atcaccctgg gtcaagacac
      961 tgccccaact acgctgaccg ctgatgacac cactattgct atgtctgcgt tcctgaaagg
     1021 cgacggcgaa gcgattgtcc caggtgcgtt caccggtgcg gcagacttcg ttctttccta
     1081 ccagtaagta gccgcttaac tgtgctggct ccctggttag cagggagccg ttaagaacgt
     1141 gaaatcagac ggaagtatgg tgatgttgcg tattgttttc ctcctgcttt tgctggtgag
     1201 ttcgacaggg cgcgctgttt cacatggtca cgggcatgta caaatgcaag gaaccattat
     1261 tgatacagca tgtgccattg ctacaggaga tgtggagcag gggatagatt tcggcgcact
     1321 tacggcaagt gaattaatta ataatggccg tggtccttct ttgccattta ccgtccattt
     1381 agtgaactgc gttcttgatg ggacagatat tcgaggcctg aatcactgga aagacgtacg
     1441 tataacattt gtaggcgatg ctgatagcga gaaccatttt gctttacagg gagatggacg
     1501 tggcgcggct ctggtcattg ctgatagcca gggggttgag gcgaaacctg gagagcctat
     1561 gccttctaca cccattacac ctgggagtat ggcattgcat tatcaattat ggctaaccgg
     1621 cgggaaaaca atccggcctg ggcatattca taccaccgtt cgctacttca tggagtacga
     1681 ctgatgttta atcagaaatc cggaaataac ggcggattaa ataacgtcag attgaattcg
     1741 ttaacgtgga tgctgttgct cgtattgggt ggtattcctg ctgcgcagtc acaagataac
     1801 atgtcaacgc cagcgaattc gaatattcca gatgaggtag tcgagcagcc gaatataacc
     1861 gagttcaaca ccgatgtgat ggacgtaaac gatcgaaata atatcgacgt tagccatttt
     1921 tcccgtgccg ggtacgttat gcccggggaa tatccgctca tattagttgt gaatggaact
     1981 gaaattcgcg aggtcacggt ttcatttgca ccgccggaag atgacccaca aggcagctcc
     2041 ccgtgtttaa caccggaaat ggtaaagctt tttggtctta aagatgaagc aacgaaaaaa
     2101 ctaatctgga aaggtaacgg tgatgatcgt tgtctggatg aaaagtcact tcccggcatg
     2161 accgctaccg gtgaccttag taccggttca cttaagattt ctttacccca ggcctggctg
     2221 gaatatagcg cgccggactg ggatccgcca tcccgcgggg ataacggagt ggcagactta
     2281 ttatttgatt acaacctgaa cgcatcgaaa agccatgcgt tatctgaagg tggttccagt
     2341 gcgaccagct taagtggtag cggaacggca ggcgcaaatt ttgggccatg gcgtttacgt
     2401 gccgactggc aagcctcgcg ttatagcgac aggggataca cgtcgcagag tatggactgg
     2461 agtcgatttt atctctaccg cgcgcttcct aagcttggcg ccaggttaac tctgggtgaa
     2521 tctgatcttg attcatcact gtttgatagc ttccgcttta tcggcggtag tctggtgact
     2581 gatgacaata tgctgccgcc taatcttcag ggatatgctc ctgaagtgac gggggtagcc
     2641 aggtcaaatg cgaaggtcgt gataagccag caagggcgcg tgatatatga atcgcaggtg
     2701 gcagctggtc cttttcgtat ccaggaatta agcagttccg tcagcggtac cctggatgtc
     2761 cgggtggagg agcaagatgg acgggtacaa cattttcagg ttgatacggc cagtattccc
     2821 tatctgacgc gtccggggtt ggtgagatac aaagcgtcct taggaaagcc tacggactgg
     2881 aaacaccatg tacaggatgt gtcttttgcg acgggagagt tctcttgggg tatgacaaac
     2941 ggttggtcac tatttggtgg cctggtcgca gccaaaaaat atgcggctct tgcagtgggt
     3001 attggtcgcg atttgctgtc atttggtgcg gtttcgtttg atgtcaccga atctaaggcg
     3061 acgcttgata ctggcgaaaa aaaaatgggt ggttcatacc gcctgagtta ttccaaacgc
     3121 tttgagaaaa tagatggaca gataacgttc gcgggctatc gcttttcaga aaaagacttt
     3181 atgtcaatga acgattttct cgaaacagag tatgacgccg gtagcaggag tgatactgcc
     3241 tggggaaggc agaaagagct ttacaccatc acagtcagta aagcttttcc tgactggaat
     3301 atgaatctgt atggcaatta tagccaccgg acctactgga atcgtcccga tgcagatcgc
     3361 tatagcctgt ctttatcgaa atacatggac gttgggcgtt ggaaggggat tagcctgaac
     3421 ctgactggct atcgcagtga ggcatggggt gtgaaggatg atggtgtata tgccagtctg
     3481 agcattccct ggagtgatgg cggttcattg agtttcaacg ggcaaagcac aacggctggc
     3541 acaacacaga tggttggttg gtacgacaga attgatagta acaataatta cagtctgcaa
     3601 gccggtacaa gtgttgggaa taaggcctcg gcgagtggat atgtgtcaca caatggtgat
     3661 ctcacgaatg gaacgctgag tgcgagttat tacgaaggaa acaatacgtc gttagggctg
     3721 tctttgcagg gcggaatgac agtaactcgt tacggagcgg cgttgcatcc tggtgcgatg
     3781 aacggcggta cgcgactgat gattgattct ggtgatgttg ctggtgtgcc gttaagccat
     3841 agtggggttc tgcaacgcac gaatgccttt ggcgtcggcg tcattggtaa ccttaatagc
     3901 tactggcgca acagtgttgg ggtcgatgtt aatgccctgg acgatgatat ggaagtcagc
     3961 catccgttaa cggaattgac catgactgaa agtgcgatcg gttatcgaaa aatcgatgtt
     4021 cttgaaggtc gaaaattgat ggcggtagtg aagtttgcgg atggtagtag tccggcattt
     4081 ggcgccaccg tctggagagg gagccatcag accggcgtcg ttgatgataa cggtagcgtg
     4141 tggttagccg gggttaagcc gggtgtcacg atggaagtcc gtgaagatga aaatccggta
     4201 tgtcaggtag gtattcctga gcgattaccg ccggaaaata ccgatcaatc gctgttatta
     4261 acatgccagc agaaatcata actcattatg tgagtggggc actgtattgg aaaaacagtg
     4321 tgtgtagaga ttaggagaaa aggacgcggc catggacggt acattaatgt tgataattaa
     4381 attcagagaa tcaaatgaac agaaataaat caattaagtc ccagccgttc ctgaaatctc
     4441 ttctggtcat ggctttagtc agtcagtccg catttcaggt tgctgatgcg gcagttgctc
     4501 tggatcgtac tcgggtaatt tttgaaggcg gtaataagag catgagctta aatattcgta
     4561 acgataatac gaagctccct tatttagcgc aatcttgggt agaggatgag caaggaaaca
     4621 aaatacagtc accgctgatg gcattgccgc cgctacaacg tatggagccg ggttcaaaaa
     4681 gcctggtgaa aatacagtta gcaccaggcg ttgaagcaaa attgcctcag gatcgtgaat
     4741 cgttgttcta ttttaattta cgcgaaattc cacctaaaag taataaagcg aacgtcctgc
     4801 aactggcgct acagactcgg gtgaaaatgt tttatcgccc gaaagcgctg gcaaaaaacc
     4861 aggcaatgaa tgaaacaccg tggcaggagc agttgacgct t
//
LOCUS       DADLSE010000223         4893 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.224, whole genome shotgun sequence.
ACCESSION   DADLSE010000223 DADLSE010000000
VERSION     DADLSE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.224"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(404..1495)
                     /locus_tag="JN692_003949"
                     /pseudo
     CDS             complement(404..1495)
                     /locus_tag="JN692_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1527..2072
                     /locus_tag="JN692_003950"
     CDS             1527..2072
                     /locus_tag="JN692_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7266095.1"
                     /translation="MKDVKVMPESIPAGYEVLQELDELDSLLIIDLGGTTLDISQVMG
                     KLSGISKIYGDSSLGVSLVTSAVKDTLSLARTKGSSYLADDIIIHKKDNNYLKQRIND
                     ENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHVMVIGGGAELICDTVKKNTQIRNE
                     RFFKTNNSQYDLVNGMYLIGN"
     gene            2075..2426
                     /locus_tag="JN692_003951"
                     /pseudo
     CDS             2075..2426
                     /locus_tag="JN692_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein stbB"
     gene            complement(2576..2749)
                     /locus_tag="JN692_003952"
     CDS             complement(2576..2749)
                     /locus_tag="JN692_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266096.1"
                     /translation="MLSGQIFCIPLNNLVGDKINYDEITKITARDWRQYRAPGWQITH
                     QKRYCQTLRTHQF"
     gene            complement(2802..3077)
                     /locus_tag="JN692_003953"
     CDS             complement(2802..3077)
                     /locus_tag="JN692_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7266097.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(3077..3361)
                     /locus_tag="JN692_003954"
     CDS             complement(3077..3361)
                     /locus_tag="JN692_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HAY7266098.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            3813..4064
                     /locus_tag="JN692_003955"
     CDS             3813..4064
                     /locus_tag="JN692_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266099.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
     gene            4033..4323
                     /locus_tag="JN692_003956"
     CDS             4033..4323
                     /locus_tag="JN692_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY7266100.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(4423..4608)
                     /locus_tag="JN692_003957"
                     /pseudo
     CDS             complement(4423..4608)
                     /locus_tag="JN692_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1267 a         1183 c         1175 g         1268 t
ORIGIN      
        1 ttatacgata attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc
       61 gtgatccgat ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg
      121 ccgggcaccc ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct
      181 cagaagtact gagtcgggtg atcgcgtcag gccgcgaagt actgacacgg gtattggtga
      241 ctggtgatga agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt
      301 cagtccgcgt ggttgctggc ctagctgatt tcctgttgtc aggcgttcaa caaccgactg
      361 caggttatag cccggttcac gaacacctga cggcgggtat cactcacccg gtgaaggtaa
      421 atccctcaag aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca
      481 ggacgtccgt cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc
      541 tcattatctg tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc
      601 gtcccataac aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc
      661 cgtcacccag gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat
      721 ttaatggccc gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat
      781 tcactatcgg ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac
      841 ggatgagttt ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg
      901 gctccctgct ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg
      961 ctgatgtgat gccgggatga ttatccgcca gagatttgta cacgctgtat ccgtcactca
     1021 ccagcgtacc gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg
     1081 tctgcgagtc aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca
     1141 gccagccgga gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat
     1201 ctgcatgaat aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca
     1261 gtgcagctcc ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca
     1321 ttcgggagaa aatgtgctgc tggcgataca ggggcagatg gtcggtatat ttgctgatga
     1381 caacctgtgc aattaccgac gactcaacag cactttcagg gaggatatgc gccggcattt
     1441 tcccgctgaa cactttctca cagcagggac agctgtactg cggacggata tagcggattg
     1501 cattaaatgg tggggataca ttcacaataa aagatgtaaa agtcatgcct gaatctatac
     1561 cggcaggtta tgaagttcta caagaactgg atgagttaga ttctttatta attatagatc
     1621 tcgggggcac cacattagat atttctcagg taatggggaa attatcaggg atcagtaaaa
     1681 tatacggaga ctcatctctt ggtgtatctc tggtcacatc tgcagtaaaa gatacccttt
     1741 ctcttgcgag aacaaaagga agtagctatc ttgctgacga tataattatt cacaaaaaag
     1801 ataacaacta tctgaagcaa cgaattaatg atgagaacaa aatatcaata gtcaccgaag
     1861 caatgaatga agcacttcgt aaacttgagc aacgtgtatt aaatacgctc aatgaatttt
     1921 ctggttatac gcatgttatg gttataggcg gtggcgcaga attaatatgc gatacagtaa
     1981 aaaaaaacac acagattcgt aatgaacgtt ttttcaaaac caataactct caatatgatt
     2041 tagttaacgg tatgtatctc ataggtaatt aataatggac aagcgcagaa ccattgcctt
     2101 caaactaaat ccagatgtaa atcaaacaga taaaattgtt tgtgatacac tggacagtat
     2161 cccgcaaggg gaacgcagcc gccttaaccg ggccgcactg acggcagatc tggccttata
     2221 cagacaagat ccccggcccc ccatttcttt tatgtgaact gctgacgaaa gaaaccacat
     2281 tttcggatat cgtgaatata ttgagatcgc tatttccaaa agagatggtc gattttaatt
     2341 cttcaacaat cactcaatcc tcttcacaaa aagagcaaaa aagtgatgaa gagccccaaa
     2401 agaacgtgat gaagctaata aactaattca attattattg agtttccttt atccactatc
     2461 aggctggata aaggaaactt aatcaagtta ttttcttgcc aatcattaca taaaataaac
     2521 agggtcgtta ttatgaaatt aatccacggt gcttatgaaa aagcggctcc aataatcaaa
     2581 attggtgagt tcgcaatgtc tggcaataac gtttctgatg ggttatttgc cagcccggag
     2641 cgcgatattg ccgccagtca cgagcggtaa ttttcgttat ttcatcgtaa tttattttat
     2701 ctcctacaag attatttaat ggaatgcaaa atatctggcc tgataacaat gctccagaat
     2761 taatacctgc aggctatgtt aaagttgcct ccacacccaa tctacctgtt ctctcgtgtg
     2821 tgccacaggc gcaatacata aatagtctgg ccagtaagtt cgtaacggat ttcgtactcg
     2881 ccagtaaaaa tccgtctgac ctccctgggt tcaaactgaa acaactgttc tcccatacgt
     2941 ggatgagtta acagaatgac cggcgcctgt gtcagggact gcaccgttct ggcggcagca
     3001 ggtttactgg ccagcaccag aaaatcatat aaccgcgcca aatcagaaag tgccttactg
     3061 gtccacttca gttccatcag cgtggcaccg gtaacggatg gtcagtactg aggctgtccg
     3121 cccaggcctg tacagcctga tggtcgataa cctgtccgga tgtcacatcg tccagggctt
     3181 ccagcgtcag gcgattacgc tcctcctctt gggcaagcca tgcagaaagc gcctgtttga
     3241 taacccagcc ccgggagcgt tccagtctgg cggccatctg gtcgacttta tcagccatag
     3301 gtaatggcac atgcgcggta attacttttg ttgcttgtgc ggtattgttt ttcatttgca
     3361 tacccgatta gttgttaatc atatttaatc atagtgatta ctttcagacg gaacaaggca
     3421 aataatgcga gtcagcgcat ataaggcggc atacggtggt tacatgtagc acaggtgtaa
     3481 aaccgccgaa gcccggcgca agccggtacg gtcgtccgaa tatcaccagt ccccctccgg
     3541 ctctgcgagg ccatgcggcc tgtcagcgat gaaaatgcct ctgtgcgcgg tcggagtggt
     3601 cccgacaagg gtttacccgc agtcgaggcg tatctccgcg ttagcgggcc gtgagggccg
     3661 cttacgagcg tgtatgagag acgtacagta gttttgtccg ctcacaccag tacaaaccag
     3721 tcgaaatacc ggtaacgacg attactcttg tctctgatgc cggtggacta cgagatttta
     3781 tttactggcg tcctgatgcg gtcaggactg gtatggaacc tgtttatgtg atacttaatg
     3841 ctcttctgga ttccgggcga tttacgcgta aacttattct gttagggctg tcaggctcat
     3901 tttcctatat cttcggctca attgttgcga cactgggaat gggacttgtt gtcgattatc
     3961 tgggatgggg ggccacattt atcgtactga tactgtctgc tgtttttgcc atcattttca
     4021 cactgatgag tcgtgaacgc tcactggagt tcgaaaaaga gtaatttttt caggaaaaac
     4081 ataaaattac tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat
     4141 atcgtgtcac ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga
     4201 tataaggcgc ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata
     4261 atggatgctg aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa
     4321 taaggagtga gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac
     4381 acaaatcccc acccggggat ttgtgatgtc agatgtcccc tgtaaaacta tgttttcatt
     4441 gcgctctatg aagctgagtg agcgtaacga ctggagctgc ttctgcggtg ctccggtggc
     4501 gaacgtgaag tcatactctt cgaacatttt caccgccggg aaggctacca ttcgggtata
     4561 catcgcctgt ttacgttgat gacgtgccag tttttcttca tgaagcatga tgcttcaaga
     4621 agggcactgt tgcaaagtta tcgatgaggt agctgaccat cactgctgtc ggaatcgatc
     4681 ttgcccttct gtttccagtc gcctcagcaa actatttttc agttaccacc tgtcctgcgg
     4741 taagcgtaaa ccgaccaccg tatgtagcca ttagacaaga attggtaact tagacgccca
     4801 tctgacatag acgaacacct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
     4861 agtaagcgcc ccatcagcga cgtcttgtga aaa
//
LOCUS       DADLSE010000224         4697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLSE010000224 DADLSE010000000
VERSION     DADLSE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.225"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            229..1251
                     /locus_tag="JN692_003958"
     CDS             229..1251
                     /locus_tag="JN692_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7266101.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1251..2231
                     /locus_tag="JN692_003959"
     CDS             1251..2231
                     /locus_tag="JN692_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7266102.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2228..2986
                     /locus_tag="JN692_003960"
                     /pseudo
     CDS             2228..2986
                     /locus_tag="JN692_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2996..3805
                     /locus_tag="JN692_003961"
     CDS             2996..3805
                     /locus_tag="JN692_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7266103.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            3805..4659
                     /locus_tag="JN692_003962"
     CDS             3805..4659
                     /locus_tag="JN692_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7266104.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
BASE COUNT         1060 a         1280 c         1183 g         1174 t
ORIGIN      
        1 gtggacgata aaaaacaata atgtcattca tatttaaatg atttagcctt aaatgattta
       61 aataatatta gccacctgat aaatacattt ttaatgatag agagggtcaa caggcttgcg
      121 gaagtcgtca catcttcatg ttttcaatag ctaaactttg atattgaaca tgatttttat
      181 cgatatatat gaaagtatat atcgattttg cttttcagga tgttcaccat gcccataacc
      241 agacggacgt ttgcgcaggc acttgcatct accctgctcc tgcagtctct accgtccttt
      301 tcacaaacag ttaatcgttt tgcttcgcaa tctcttcccg aagcccaaaa cattacacgc
      361 atcgtcagcg caggcgcgcc cgccgatttg ctattgctgg ctgtcgcgcc agaaaaaatg
      421 gtcggttttt cctcttttga ttttgcccgt caggcattaa ttccattgcc agagcacatt
      481 cgccagttac ccaggctggg acgactcgcc gggcgcgcca gcacactatc gctggaaggg
      541 cttatggcgt tacatcccga tttggtcgtt gattgcggca atacggatga aaccttgatc
      601 tcccaggcac ggcaggttag cgaacagaca caaatcccct ggttattgct taacggaaaa
      661 ctggcgcaat cagcagaaca gttaacaacg cttggcaaaa cgttaggcga agagcaccgc
      721 gccgcagaac aagccaatct cgccagccac ttcgttggtg aagctcaggc attcgccacc
      781 tcacccgccg ctaacctccg cttttatgct gcgcgcggtc cccgagggct ggaaacgggc
      841 ttacagggtt cgttgcatac cgaggcggcg gaattattag gtttgcacaa cgtcgcgcaa
      901 atagccgatc gccacggcct gacacaggtt tccatggaaa atctcctgcg ctggcagccg
      961 gatattattc tggttcagga ggccgttact gcagatttta ttcgtcgtga tcctctctgg
     1021 cagggcgtga aagccgttgc ggaacaacgc atcttatttt taagtggctt gccctttggc
     1081 tggctcgatg ccccgccggg aatcaaccgt cttctgggat tacgcagact tcacgcctgg
     1141 ctggatcccg ccatcaatcg ccagtttaaa agtgacatgc agcattacgc ccaactgttc
     1201 tggcattgtt cactcagtga tgccgactat caaaaattgg tggcgagcta atgagaatcg
     1261 taaacggatg tattttgctg ctggcagcta tcagtatcac ttttgccgcc gtaagtgggg
     1321 cttatcatct cgatatgcag caactgctgg cgctaattct tcgtcaagaa aatgtgtctg
     1381 tgcaggaaca aattgtcttc tggcaaatcc gcgtgccgcg tattcttgct gcgctgttac
     1441 tgggcgcagc gctggcagga gccggaacca cctatcaggg aatgttgcgt aatcccttag
     1501 tttcaccgga tattttaggc gtctccgccg gtgccggact tggtgcctgc gctgcaatcc
     1561 tgtggggatt acccattgtt tacatccagc tgtacgcatt ttgcggcggg ctgatggtgg
     1621 tcgcgggcgt ctggttgatt acgcgtcggg tgacccgcca cgatccgatc ttaaccctgg
     1681 tgctggtagg cattgcgtta ggaacgttgt gcggagcggg gatatcgcta attaaaacgc
     1741 tggccgatcc ttacacccag ttaccgtcaa ttaccttctg gttgctgggg ggactgtcca
     1801 ccgtgacctt acgcgatctc tgctatgctg caccgatcat tctcataggc tccttgcccc
     1861 tgttttttct ccgctggcgc atgaatttac tcacgctttc cgacgacgaa gcccgttcgc
     1921 tgggactgaa cgtgacgcgt ctgcgctttg gattgattgt ctgcgccaca ctaatcaccg
     1981 ccagcacggt agcaatagcg ggcataattg gttggatcgg tctggtcgtt ccgcacattg
     2041 cccgcttgtt gaccggtcat aaccatcagc agctgttgcc tatggcaatg tgtactggcg
     2101 ctattctgct cctgcttacc gatacgctgg cgcgcagcat tggaacaaca gaaatcccac
     2161 ttggcattct gaccgctttt gttggcgcac cgtttttcct gcttttgttg ttacgcggag
     2221 gtcgccagtg actttgcttg ccgttcgtca tgcgtctctg ggttactccc gccatccagt
     2281 gctacgcgac gtgtccttca ccctgtcgca aggaacaatt tgttgtctgc ttggcgcgaa
     2341 tggttgcggc aaaaccacac tcatgcgctc cattcttggc gttataccgt tattaaaagg
     2401 cgagatccta ctggatagcc tccctgtgca gacactcagc catcgccagc gcgcacaggc
     2461 tattgcctag gtgccgcagg cgcacgacgg tatttttgct ttcagtgtgc tggatatggt
     2521 gttgatgggc ctggccccca ccataggcgc tttttccgtt cccggtaagc aagaacgcct
     2581 gaaagccacg gagcaactgg aaaagctggg cattttgcat ctggcgacac gacgctggaa
     2641 tacgcttagc ggcggtgaac gtcagctggc tttaatcgcc cgtgcactcg tccaacaacc
     2701 gcgcttgttg ttactggatg agcccgcctc cagcctcgat ttcggtcatc agatccaact
     2761 tctggacacg ctggcgcagc tcaaaaataa cggcatgaca atgttaatgt cgactcacca
     2821 tccgctccat gccaacgcca ttgccgacag cattatccag gtagagccag atggacgagt
     2881 aacacaaggc ttaccgaccg aacagttaac aacgaacaaa ctggcggccc tgtatcgggt
     2941 ttctgccgac caaattcatc atcacctctc tgcaattagc cgctaaggaa caatcatgct
     3001 cattgatgat attgattttg cagaccttta tctgcagcaa ctcaaactgg ctcaccgcac
     3061 agaaaaaacg cccgatcact gggatcaacg ggcggagaaa atggcggaaa attgcgcctc
     3121 gccaacggac agctacctgc aacaactgat cgctaaaatt gacctgcaag gcgctcagac
     3181 cttattcgat atgggctgcg gaccaggcac cgtatcgctg gcgctggctg acaaactcac
     3241 tacggtttat ggcgttgatt acagtcaggg gatgctgaac gttgccgccc gccgcgccgc
     3301 cgcactgaag gctgacaatg tccactggat ccagcgcgcc tgggaagagg actggagtga
     3361 cctgccacgc tgcgatattg ccgtggcttc ccgctccact ctggtggctg atatgcgcca
     3421 tgcaatgagc aaactgaata atcaggcacg gcttcgcgtc tatacaactc atctggtcag
     3481 cacctcgttc gtctctccag ccattcagcg ggcggctggt cgggaagtga tcgaattacc
     3541 caactatatc tttgcgctca acgtcctgta tcagatggga atttatgccc acgttgattt
     3601 tatccgtgga caaaattgcc aacaggataa cagcacctgg gaacgttttg agcagaacgt
     3661 cagctggagt cttggcgcgc ttaacgacga tgagcgtgag cggttatatc actggtatca
     3721 acaacaagat gctcgtgccc tcgcccctgc cagccgcgac tgggcgctca tctggtggga
     3781 tagcgtaccg caggaggcac tgtgatgatt ttcctttctc aggcacaaat cgatgcgtta
     3841 ctgctggaag atatccaggg aggtgacctg accacgcgag cattaaatat tggacaccag
     3901 cctggctata tagcgttttt tcaccgtcag ggcggctgcg tcagcggcgt ttctgtcgcg
     3961 tgtaagatgt taactacgct gggattaact attgatgacg cggtcagcga cggttcacaa
     4021 gcgaacgcgg gtcaacggct aatccgtgcg cagggtaatg ccgcagcact tcatcaggga
     4081 tggaaggcag tccagaatgt gctggagtgg agttgcggtg tttctgatta tctcgctcaa
     4141 atgctggcat tgcttcgtga acgttatcct gacggcaata tcgcctgcac ccgaaaagca
     4201 attccgggca cccgcttact ggcttcgcag gcaattctgg ctgccggagg actaattcat
     4261 cgtgccggat gtgcggaaac catattattg tttgccaacc accgccattt tcttcatgac
     4321 aatcagggct ggtcaggcgc aatcgctcag ttacgtcgcc acgcacccga gaagaaaatt
     4381 gttgttgaag ccgacacgcc gaaagaggct atcgccgcgt tacgcgcaca accagacgta
     4441 cttcagctcg acaaatttag tccgcaacag gcaactgaaa ttgcgcaaat agcgccgtcg
     4501 ctggctcctc actgcacgct ggctctgacc ggcggaatta atctgacgac actcaaaaat
     4561 tacctcgact gcggcattcg cctttttatt acctccgcgc cttattacgc ggcaccagct
     4621 gacattaaag tcagtctgca acccacagcc tctatttaat tctttgttaa acggaattta
     4681 ttctatgaga ctcaaaa
//
LOCUS       DADLSE010000225         4675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLSE010000225 DADLSE010000000
VERSION     DADLSE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.226"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            118..3012
                     /locus_tag="JN692_003963"
     CDS             118..3012
                     /locus_tag="JN692_003963"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7266105.1"
                     /translation="MVVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRH
                     RNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTV
                     MMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESE
                     TRFRNAMEYSAIGMALVGTEGQWLQSNKALCQFLGYSQEELRGLTFQQLTWPEDLNKD
                     LQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINEL
                     KRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVW
                     YECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIAVKDGIRHIRALANRVLNKEGEVERL
                     LGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWT
                     QEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRNGGSYDVHYSIT
                     PLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTV
                     NSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEF
                     GLLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMS
                     QADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMIAHGVASP
                     RIPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQQAAKAVA
                     SKGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPAEAILDHAESVQKLR
                     LAGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQR
                     LGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(3122..3970)
                     /gene="alkA"
                     /locus_tag="JN692_003964"
     CDS             complement(3122..3970)
                     /gene="alkA"
                     /locus_tag="JN692_003964"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7266106.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4104..>4675
                     /gene="yegD"
                     /locus_tag="JN692_003965"
     CDS             4104..>4675
                     /gene="yegD"
                     /locus_tag="JN692_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7266107.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQLPEAITQAVIGRPINFQG
                     LGGDEANAQAQGILERAAKRAGFKDVVFQY"
BASE COUNT         1089 a         1221 c         1264 g         1101 t
ORIGIN      
        1 gcgccccacc agacccggtg acatcacccc ccggatcatg gacgactggg ggcagtaatc
       61 atgcctggtc tggctggcta acaccccatt ataaggggta cgaataaagt aacggggctg
      121 gttgtcctgc tgacgcccgg agacgatcct ctcagggcat ttttgatatg ggtactgtca
      181 gaatccatcg gcgctctggc actggtgccg ctgggattgt tatttaaacc acactatctg
      241 ctgcgccatc gcaacccacg gttgcttttt gagtcgctgc tcacgttagc catcacactg
      301 acgttaagct ggctttcgat gctgtatctg ccgtggcctt ttactttcat tattgtgctg
      361 ttgatgtgga gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg
      421 gtgatgatgg tgtcgctgat gatggccgcg gatccctccc tgcttgctac gccgcgtacg
      481 tacctgatga gccatatgcc gtggctaccg tttttgctga tcctgctgcc cgccaacatc
      541 atgaccatgg tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc
      601 cgctttcgga acgcgatgga atattccgct atcggtatgg cgttagtggg caccgaggga
      661 caatggctgc aatccaacaa agcactctgc cagtttctcg gttacagtca ggaagagctg
      721 cgcggactca cctttcagca actgacctgg ccggaggatc tcaataaaga tctccaacag
      781 gttgaaaagc tgataagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac
      841 cgcaatggcg atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca cacggatggc
      901 acgccgctct attttatcgc tcagattgaa gacattaacg agctaaaacg caccgaacag
      961 gtgaatcagc aactgatgga gcgcatcacg ctggctaacg aagcgggcgg gattggcatc
     1021 tgggagtggg agctgaagcc gaatattttt agctgggata agcggatgtt cgagctgtat
     1081 gaaattcctc cgcatatcaa accgaactgg caggtgtggt acgagtgcgt gctgccggaa
     1141 gatcgccagc atgccgaaaa agtgattcgt gattcgttgc aatcacgctc gccctttaag
     1201 ctggaatttc gcattgccgt gaaagatggc atccgccata tccgcgccct cgccaaccgg
     1261 gtactgaata aagaaggcga agtcgaacgc ctgctcggca ttaatatgga tatgaccgag
     1321 gttaaacagc ttaacgaggc attgtttcag gaaaaagagc gcctgcacat tacgctggat
     1381 tccatcggtg aagccgtggt ctgtattgat atggcgatga aaattacctt tatgaatcca
     1441 gtggcggaga agatgagcgg ctggacgcag gaagaagcat taggtgttcc gctcctgacg
     1501 gtgttgcata ttacttttgg cgacaacgga ccattaatgg agaacattta cagtgccgac
     1561 acctcacgtt ccgcgattga acaagatgtg gtgttgcact gccggaacgg cggcagttac
     1621 gacgtgcatt acagtattac gccgttaagt actctggacg gcagcaatat aggctcggta
     1681 ctggtgattc aggacgtcac cgaatcacgc aaaatgctgc gccagctgag ctacagcgcc
     1741 tcccatgatg cactgacgca tctcgccaat cgcgccagtt ttgagaagca actgcgtatc
     1801 ctgctgcaaa cggtaaacag tacacatcag cgacatgccc tggtgtttat cgatcttgat
     1861 cgctttaaag cggtgaatga cagcgccggg catgcggcgg gtgacgcttt actgcgcgaa
     1921 ctggcgtcgt tgatgctgag tatgctgcgc tccagcgacg tgctggcgcg actcggtggt
     1981 gatgaatttg gtctactgtt gccagactgt aatatcgaaa gtgcgcgttt tatcgctaca
     2041 cgcattatca gtgccgtgaa tgattatcac tttatatggg aaggccgtgt gcatcgggta
     2101 ggtgccagtg ccgggattac cttgattgat gacaacaatc atcaggcggc agaagtgatg
     2161 tcgcaggctg atatcgcctg ttatgcctcc aaaaatggtg gacggggccg ggtgacggtt
     2221 tacgaaccac agcaagctgc cgcacatagc gaacgggcgg cgatgtcgct tgatgaacag
     2281 tggcggatga ttaaagagaa tcagttgatg atgatcgccc acggtgtcgc ttcgccgcgg
     2341 atcccggaag cgcgtaattt gtggctgatt tcacttaagc tctggagttg cgaaggcgag
     2401 attattgatg aacaaaaatt tcgtcgtagc ttcagcgatc cggcacttag ccatgctctt
     2461 gaccgacggg tattccacga atttttccag caggccgcaa aagcggttgc cagtaaaggc
     2521 ataagcatcg ccctccccct ttccgttgcc ggtttgagta gcgccacgct ggtgaatgat
     2581 ctgcttgagc agctggaaaa tagccctcta ccaccacggt tattacatct gattattccg
     2641 gctgaagcga ttttagatca cgcagaaagc gtgcaaaaac tgcggctggc gggatgtcgg
     2701 atagtgctca gccaggtggg ccgcgatctg caaatcttca actcgctgaa agcgaatatg
     2761 gcagattacc tgctacttga tggtgagtta tgcgccaacg tgcagggtaa tttgatggat
     2821 gagatgctga ttacgattat tcaggggcac gctcagcgac tcgggatgaa aaccatcgcc
     2881 gggccagtcg ttttaccctt agtgatggat acgctttctg gcatcggcgt cgatctgatt
     2941 tatggtgatg tgattgccga tgcccaaccg ctggatttgc tggtgaatag cagttatttc
     3001 gcgattaact gagagagcgt ttgcccattg cctgatgcga tgctaacgcg tcttatcagg
     3061 cctacaaatc gctcattccg caggcgggat aaggcgctcg caccgcttcc ggcgaccaac
     3121 gtcatgcttc gtctggttgc cagccttccg tataccagat atgcaacagc gcataagaac
     3181 gccagggctt ccagcgctcg gcataacggc ggatttgtgc cggtgtcatc cccagaaatc
     3241 gctgtttaat cagataatca tccggcagaa aaacatcttt cgcctgccag ccgcgcaagg
     3301 cgaaataatt cgccgtccag cgcccgatac ccggaaaagt ttgcagcgtt ttcatcgcct
     3361 gctccacatc gcccggtatt gtcattggta aggtgccctc cagcgccgca tttgccagat
     3421 gaatcagcgc ctctgcccgt ttcaacggca tacctaacgc ttttaatgcc tgcgggtcgg
     3481 ctgctgccag ccgctgaggc gtggggaagc agatatactc cggaaaatca tccagccgtt
     3541 cgccataaag ctgtgccact ctggcggtca attttgccgc catcgccacg ctcaccagtt
     3601 ggcctaaaat cgcccgcacg ccctgctcaa aagcatcaac actgccgggt aaacgcaatc
     3661 cgggccgcgc cgcgcctaac ttgcccaacg caccgttaac aatctgcggg ttacattgca
     3721 gatcaagcag gcggctcatt ttcgccagac actcagcggc aacgggttct aaacctgcac
     3781 ttaaatttat gtgcagagta tggcgggcta tatccggaat agcagtcacc acgccgcgat
     3841 attcgcccac tgccagacta cgggcataat aactgtccgc gaccgtttcc acaccactca
     3901 ccgcacgggc ggcgagaaat cccaacatcc acgaccagtc atacggcggc tgccagttca
     3961 ggatatacat cgcatctcct tattcatccg ctttcagcat aaacgttatt cagacgttac
     4021 gctttgcttt catattccgg ttgtcgcgac ggcaacattt cgctaaagtc acgccccttc
     4081 ttcaccggca tggggattat ttcgtgttta ttggttttga ttacggtaca gcaaactgtt
     4141 cagtggcggt catgcgtgac ggtaaaccgc atttgctaaa aatggaaaac gacagcacgc
     4201 tgctgccttc aatgctttgc gcgccaacgc gtgaagcggt aagcgaatgg ctgtaccgcc
     4261 atcatgacgt tccggcagac gacgatgaaa cgcaggcgtt gttacgacgg gcgattcgtt
     4321 ataaccgcga agaagatatc gatgttacag cgaaaagcgt gcagtttggt ctttcctcac
     4381 tggcacagta cattgatgat ccagaagaag tgtggtttgt gaaatcacca aaatcgttcc
     4441 tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt tgaggatctg gtctgcgcaa
     4501 tgatgttgca cattcgccag caggcgcagc tgccagaagc gattactcag gcggtgattg
     4561 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4621 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacga
//
LOCUS       DADLSE010000226         4669 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLSE010000226 DADLSE010000000
VERSION     DADLSE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.227"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            464..1150
                     /locus_tag="JN692_003966"
     CDS             464..1150
                     /locus_tag="JN692_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7266108.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1143..2036
                     /gene="virB9"
                     /locus_tag="JN692_003967"
     CDS             1143..2036
                     /gene="virB9"
                     /locus_tag="JN692_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HAY7266109.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQPVTDADGKTVQ
                     KIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNLAFVVRFSYPDDTRRQNDAA
                     RLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAGSESITPDFAYDDGRFTYLG
                     FSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSLSPRFVLRYGSQVVGIENGS
                     YGKVTVANGSTVSPDVKLESK"
     gene            2033..3229
                     /locus_tag="JN692_003968"
     CDS             2033..3229
                     /locus_tag="JN692_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7266110.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            3233..4282
                     /gene="virB11"
                     /locus_tag="JN692_003969"
     CDS             3233..4282
                     /gene="virB11"
                     /locus_tag="JN692_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7266111.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            4269..>4669
                     /locus_tag="JN692_003970"
     CDS             4269..>4669
                     /locus_tag="JN692_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266112.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
BASE COUNT          985 a         1482 c         1407 g          795 t
ORIGIN      
        1 ataagctggc cgaggcgctt gccggtgcat ccgcacaggg tgccgctcag gcacttgccg
       61 cgtcgggagt aggttcagcc cgtcaatccg cctcctccgc agccggtgcg gtgggggata
      121 aagcctcaca gaaccttaac agcaccggca gcgcaacggc ctcgtctgcc tccacggcct
      181 cccgcgccac cggcgatgcg cgcagccgca gccgcgccgc cgttcagcgg atgcaggaac
      241 gctaccagag ataacccagt ctgactgaac tgcgggacgc cgcaacggcg tcctgcggct
      301 tcgtcacgtc tttaccacag gaaaaacacc atgaaatacg gcatcacgct gctgatgctg
      361 tgcctgcttg caggctgcgc acagcgaaac acccaacttc cgcccgtgtc gggcaacccg
      421 gagccggtca attccccggc cattattcag gagttaaccc cccatgtctg aacttaccgc
      481 caaagccctg agcgactcgc gctcgtttga gaacgccatt cttgagcggg atatgcgcat
      541 gaaaaaggcg ggctggatcg tggcggcgct cgccggtctg ctgctcgtct tcgcccttgc
      601 cgcccttatc ctgctgctac cgctcaaaac caccaccgtg gagctgtaca cgctcgaccg
      661 tcagaccggg cgcatcgagc gcgtcaccac cgtcggcgag gacaagctgg acacctccga
      721 ggtgctcaat ctctcgcaga ctgcgtccta cgtgaagcgc cgcgaggggt acaactattt
      781 cgcccttcag caggactaca gagagacgca gatgttcaac agcgacgagg tcaacaagga
      841 atacctcgac tggtttaaca gcccggacgc gccggacgct gtgtttcagc aggcggccta
      901 tgtagtcacg gtggaagtcc tctccaacgt accgtcagag ggcaccggct ccagcatggt
      961 ggagatgctg cgcatcaagc gcaccattcg aaaggtcaag gacaacaccg aaacgtatga
     1021 ttactggacg gtgcgcatga cctaccgcta tttcccgcag aagaaaatga cggcctccga
     1081 gcgcgaggtg aacccgttcg gcttcatcgt caccagttac cagcggttta aggagaaaag
     1141 cgatgaataa ggtactgatg tgtgccctcc tgctgaccgc agcgtcagca tggggggcgg
     1201 cgacaccgcg cggcagcgcc tatgacagcc gcatgcagaa catcccctac aacagccaga
     1261 acacgacagt gattaacacg cgggcgggtt acatcacgac gctggttttt gacgccgacg
     1321 aggaggtgat caccgccacc gctggttttc cgcagggctg gacgatcaca cccgaccgta
     1381 accgcgtggg gattagcccc gcgccggtga agcagccggt aacggatgcg gacggcaaaa
     1441 ccgtccagaa gattttcacc cccacgcagc aggactggca gaccaacctg tttgtgacca
     1501 ccacaaagcg cgactacagc ctgatgctga acgtgatcga taacgatcgc gaggcgggaa
     1561 atctggcctt tgtggtgcgc ttcagctacc ccgacgatac ccgcaggcag aacgacgcag
     1621 cccgcctcgc ccgtcagcag gagcttcagg ccgcgcagga gaaagaacgc attcagtttg
     1681 ccctgaagaa atccactgcg ccgcgcaact ggaactacac ccgcagggtg gcggcaggtt
     1741 cggagtcgat caccccggat tttgcgtatg acgacggccg gtttacctat ctcggctttt
     1801 caccgcagaa gctgatcccg tcgccgaccg tcatcgtcgg cggcagcgag caggtgatca
     1861 ccccgacctt cagcaccgag ggtaactacc gggtgatggt actgcgctct ctttctccac
     1921 gctttgtgct gcgctacggc agccaggtcg tggggattga aaacggcagt tacgggaaag
     1981 tcaccgtggc gaacggcagc accgtctcac cggatgtgaa actggagagc aaatgatgaa
     2041 cgaacaggaa gacattaaaa ccgccgccga actggaggcg gaagcccgcg agcgggcgcg
     2101 ggcagagatt gacggcgata aacccgccgc aggcgcggtg ccacccggac agccacaggt
     2161 ggtgcagcac gcgaagcgcc gcagccgccg ctcgctcgtg ctggccgcca tcagcctggt
     2221 gttactggtc attatcgcca ccggcgggga ttacctgatg gcgaaactga agggtgacgg
     2281 cagcggaaaa agcgcgagcc atgaaccgga atcgcacgcc acctcccccg cccgcgagcg
     2341 caataacctc ggacgggatc tgaacccgct gggacagctg gcagacacaa ccggccagga
     2401 gaagactact ccgactgata ccgccgcgcc gccgcaggca tcaccgcccc ccctgacgtt
     2461 tgacaaggtc agcgccctgg cagacagcgc cccaaccact gcaagcagcg ccccgaaaag
     2521 cacgtccagc agtaacgtat cacctgcggg cagcgcgtcc gcgccaggct ctgcaacacc
     2581 cgccgggcag tcgcaggata acgggctggc gcgcattacc ggcgtgaagc ggctcggcct
     2641 cgatccggat ttgtacattc cggccgaccg gcatatcccc tgcgcgatga tgcagcgctt
     2701 tgtatcggac gtgggcgggc gctttaactg ccttatcagc gaggacattt acagcgccag
     2761 caacgccgtg cgccttatcc cggcaggcac tgaggcgcgc ggcctgtacc gcaccggcac
     2821 gctgaaaaac gggcaggggc gtctgttcct cgccatcacc gagctgcgca cgccggaacc
     2881 cggccgcctg gttattccga tggttgacag ccaggccgtg ggggcgcttg gcgagaacgg
     2941 cgtcgcgggc tggatagaca atcactggct ggagcggata ggtaataccc tcctgctcgg
     3001 cacggtacag gactttgccg ctgcggcctc cggctcgtcg ccgggcaagg atcgcaacac
     3061 ggactacacg gagaacaccc gcgcggccac ggcggaaatg gcaaaaacgc tcctggaaaa
     3121 cagcatcaac atcccgccga cgatgtacct caaccagggt gacgtgattg gcctggtcac
     3181 gggggcggac attgattttt cagacgtcta cagactgcgc atgaggtaag cgatggacga
     3241 taaactgact gataccccgt cagagggcaa cgtcgcgctg tcacacctgc gcgatctgtt
     3301 gttcggggac tacctgagcc gggaggggct gacggaaatt gccatcaacc gccccggaga
     3361 gctgcacaca aaaatcaacg gggcgtggga aatgcacgcc tcgccggtca cgctgcgcca
     3421 gtgctcgtcc tttgccaggg cgctggccgt gtggaacaac gacaccattg atgaaacgtc
     3481 gcctatcctc tcggccacgc tccctacagg agaacgctgc cagatagtgg tgcccccggc
     3541 ctgtgagcgt aacaccatct caatcaccct gcgtaagccg gactacttcc agcgcaccca
     3601 tcagtcgtat atcgatgccg ggttttatga ccgggtgcag ggtacagaaa aggtggaatc
     3661 aaaagacggg gagctgagcc gcatatacct gtccgggaat atcccgcttt ttatggaaaa
     3721 gtgcgtggag tacggcaaaa cgctctttgt ggtgggggaa acaggctctg ggaaaaccac
     3781 ctacatgaag atgctgctgc actacattcc cgagacgctg cgccttatca ccatcgagga
     3841 caacccggag ctgaagtttt acagccagaa aaactacgtt catctgttct acccggcgga
     3901 cgccggagag ggcgcaatag tcacctctga tcgcctggtg cgcgcgattt accgtatgaa
     3961 ccccgaccgc cccctgctcg ccgaggtgcg tggccgcgag gcgtgggacg cactcaagct
     4021 gattgagtcc gggcacgagg ggtttatgtc ctccctgcac gcgggcagcc cgcgcgagtg
     4081 tatctccggg ctgattgacc gctgctacga gcatccggcg tgccggggca tgccggttga
     4141 cgtgctgctg cgcaaggtac tgcactgcgt cgacgtcatt gtgagcgtgg acgttcacgg
     4201 caacgtgcgc cgcatgagcg acgtctattt taaacccctg cacatgcagg ctatgaagga
     4261 gagtttcaat gatgctgcct aaactgaccg gcctgacggc gatcgtcgtc ctcctggcgg
     4321 gctgttcttc gccaccggag cctgcggccg ttgactggtc acaaccggcg cagccggtaa
     4381 cgaccacccg gccggtatgg gaacccaacc gtcttatcgt ggcctcgccg gtgacggaag
     4441 ggcactggtc gcaggtgctt acgcgttttg aacccggcgg gatttacccg gccgcacagt
     4501 ggtacgcggt tgcgcacgcc tcgcgcgcgg tagtaaatgc cccggacggc cagcgcttct
     4561 ttgcggccaa aacctggctc cgcgagggcg gctacaaggg tgtggtggag tttcgccccc
     4621 gctccggctg cctgacctgc gacacctcag aaatcgtctt ctaccgata
//
LOCUS       DADLSE010000227         4631 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLSE010000227 DADLSE010000000
VERSION     DADLSE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.228"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..233
                     /locus_tag="JN692_003971"
     CDS             <1..233
                     /locus_tag="JN692_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7266113.1"
                     /translation="IADHDKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVM
                     GGGEELSNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            375..596
                     /locus_tag="JN692_003972"
                     /pseudo
     CDS             375..596
                     /locus_tag="JN692_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(617..679)
                     /locus_tag="JN692_003973"
                     /pseudo
     CDS             complement(617..679)
                     /locus_tag="JN692_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1130..2542
                     /gene="ospC1"
                     /locus_tag="JN692_003974"
     CDS             1130..2542
                     /gene="ospC1"
                     /locus_tag="JN692_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7266114.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2646..2795)
                     /locus_tag="JN692_003975"
                     /pseudo
     CDS             complement(2646..2795)
                     /locus_tag="JN692_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2871..4568
                     /gene="ospD3"
                     /locus_tag="JN692_003976"
     CDS             2871..4568
                     /gene="ospD3"
                     /locus_tag="JN692_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7266115.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFAENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1619 a          832 c          860 g         1320 t
ORIGIN      
        1 taattgcaga ccatgacaaa ccgcagccgt caggctgcct actcgagtca caaggctctc
       61 tgtgtccgat atgccggcag cgcattaccc atgaaactgg ctggaacgta caccataagg
      121 tgaagaaagt tatggggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      181 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      241 agggtgtacc gggaaaccgg tatgcaccgt tcttagggga gggcagtcca gcaatgaact
      301 gtccttaccc gacaaacatc caaaacgaac ggaacgtgct gaaatccgca tataacccgt
      361 tgtaaagtga ggaaatggac acctcacttg ctcatgagaa cgcccgcctg cgggcactgt
      421 tgcagacgca acaggacact atccaccaga tggctgaata caaccgcctg ctctcacagc
      481 ggatggcggc ttatgcttcc gaaatcaacc gactgaaggc gctggttgcg aaactgcagc
      541 gtatgcagtt cgataaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg cagattccat
      601 tttcacgggc gatctacgcc acacaggctc tgggttgcag tgactgctcc attaaggcga
      661 ttttgaactc ataagggaag taacaaaaaa actgcaaaag tacaataatt atatatggcc
      721 aaactaacct ctattaaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      781 tcatcgaggg tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      841 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      901 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
      961 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tacccttatc
     1021 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1081 ttgtagggaa atatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1141 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1201 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1261 aaaaaattta aaagatagtg caaatattat taaagatttc tttaggaaaa ctatagcagc
     1321 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1381 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1441 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1501 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1561 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1621 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1681 tggcgttgaa atctctagcc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1741 tgattttggt gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt
     1801 aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt
     1861 agataaattt tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat
     1921 tatagatgta ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat
     1981 tgactttatt agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct
     2041 tgcccctgtg gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc
     2101 taggatggta agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga
     2161 ggctgttaca gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc
     2221 tctccaggct ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt
     2281 atctaatttt gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat
     2341 tgagtatcta cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa
     2401 gggattggtt gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga
     2461 taacgcaata aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac
     2521 atctgacaat aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc
     2581 acaaaatatc gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt
     2641 ttgatgggtt tcgccccgtt gtagcggtag tcgagctgga aggtcttcag gcactgctta
     2701 cagcgataga gcagcccccc tgaactggaa tggccgttcc ggataccccc ctgagtttca
     2761 gagtaacggg gacaaaccac atcaatcttt gccatcaatc atccaaaggg caaagagtac
     2821 aacaacacta agtctgcgtc acaacccatc aatgaaagga atatatacat atgccatcag
     2881 taaatttaat cccatcaagg aaaatatgtt tgcaaaatat gataaataaa gacaacgtct
     2941 ctgttgagac aatccagtct ctattgcact caaaacaatt gccatatttt tctgacaaga
     3001 ggagtttttt attaaatcta aattgccaag ttaccgatca ctctggaaga cttattgtct
     3061 gtcgacattt agcttcctac tggatagcac agtttaacaa aagtagtggt cacgtggatt
     3121 atcatcactt tgcttttcca gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa
     3181 aggctattaa tgtgcctgct attatttatt ttgctgaaaa cggttcatgg ggagatatta
     3241 ttttttatat tttcaatgaa atgatttttc attccgaaaa aagcagagca ctagaaataa
     3301 gtacatcaaa tcacaatatg gcattaggct tgaagattaa agaaactaaa aatggggggg
     3361 attttgtcat tcagctttat gatcccaacc atacagcaac tcatttacga gcagagttta
     3421 acaaatttaa cttagctaaa ataaaaaaac tgactgtaga taattttctt gatgaaaaac
     3481 atcagaaatg ttatggtctt atatccgacg gtatgtctat atttgtggac agacatactc
     3541 caacaagcat gtcctccata atcagatggc ctaataattt acttcacccc aaagttattt
     3601 atcacgcgat gcgtatggga ttgactgagc taatccaaaa agtaacaaga gtcgtacaac
     3661 tatctgacct ttcagacaat acgttagaat tacttttggc agccaaaaat gacgatggtt
     3721 tttcaggatt gcttttagct ttacaaaatg ggcattcaga tacaatctta gcatacggag
     3781 aactcctgga aacttctgga cttaaccttg ataaaacggt agaactacta actgcggaag
     3841 gaatgggagg acgaatatcg ggtttatccc aagcacttca aaatgggcat gcagaaacta
     3901 tcaaaacata cggaaggctt ctcaagaaga gagcaataaa tatcgaatac aataagctga
     3961 aaaatttgct gaccgcttat tattatgatg aagtacacag acagataccc ggactaatgt
     4021 ttgctcttca aaatggacat gcagatgcta tacgcgcata cggtgagctc attcttagcc
     4081 cccctctcct caactcagag gatattgtaa atttgctggc ctcaaggaga tatgacaatg
     4141 ttcccggact tctgttagca ttgaataatg gacaggctga tgcaatctta gcttatggtg
     4201 atatcttgaa tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta gaagcgaaag
     4261 attctaatgg tttatctgga ttgtttgtag ccttgcataa tggatgtgta gaaacaatta
     4321 ttgcttatgg gaaaatactt cacactgcag accttactcc acatcaggca tcaaaattac
     4381 tggcagcaga aggcccaaat ggggtatctg gattaattat agcttttcaa aataggaatt
     4441 ttgaggcaat aaaaacttat atgggaataa taaaaaatga aaatattaca cctgaagaaa
     4501 tagcagaaca cttggacaaa aaaaatggaa gtgattttct agaaattatg aagaatataa
     4561 aaagctgaat attatctcgt ggtgatacac tgaatttgag gtaaccggct catttaaacc
     4621 gtctggtctg t
//
LOCUS       DADLSE010000228         4616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLSE010000228 DADLSE010000000
VERSION     DADLSE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.229"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            20..178
                     /locus_tag="JN692_003977"
                     /pseudo
     CDS             20..178
                     /locus_tag="JN692_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(234..1169)
                     /locus_tag="JN692_003978"
     CDS             complement(234..1169)
                     /locus_tag="JN692_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7266116.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKKINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1180..1413)
                     /locus_tag="JN692_003979"
     CDS             complement(1180..1413)
                     /locus_tag="JN692_003979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266117.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSHIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(1406..2680)
                     /locus_tag="JN692_003980"
     CDS             complement(1406..2680)
                     /locus_tag="JN692_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7266118.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELQ
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICNVSIEINADKISSITNLQLTLELKGGGGSE"
     gene            3073..4443
                     /locus_tag="JN692_003981"
     CDS             3073..4443
                     /locus_tag="JN692_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266119.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
BASE COUNT         1229 a         1074 c          938 g         1375 t
ORIGIN      
        1 tttttgaaca tgtaaaaaaa ttcaccaggc attaccaatt aaaggggttt atcttgatgc
       61 tgaccctgcc gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc
      121 aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat
      181 tattatcgcc acccgctagg gcaagttttg ccttagccgg gggctgagca gcgttaccac
      241 ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat agttgccgtt
      301 gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc cggtttataa
      361 agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc
      421 agccggttta aaccgaaagg cgcgaagcca accgtttggt taatcttctt accggatggg
      481 cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt ggtggtggcg
      541 aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatcccgcat cccagaacgg
      601 gtaaccgtga tcacgggtaa cgtatcctca accgtttttc tgtcaatcat gtcttttcga
      661 caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc aaggtattcc
      721 ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacatcat gcccaacctg
      781 gaccatgcta aattcatacc ttgctgcgcg gcctgattaa atagtgcata cgctttggca
      841 taatccaccc gcacctgctc accgtcgtag tagatactcc ccagcgctaa cggagcttct
      901 ctgtcacctt gtttagcggc taactcgaac catttaatcg cttcctgaac atcaggtttg
      961 gtatcatagc gaggctgata atattcccag cctaacaggc tctgcgcgtc gatatctccc
     1021 gattcagcca ttttatgcag tgtatttata acatcgggag cagatttacc accgtatatg
     1081 ctgtcataca attcatgcgc tttcttactt gccaccacgc ctgatgaaac tatcagcatt
     1141 aaagaaaata atattctatt aggcaacata aaatatctct tatttatctt gtacctgaat
     1201 atgctcttct tttaataaat cacgttgttt tccattcagt atcgctgtcg cagatttaat
     1261 agaataagtc aaatcatgaa aatcgcataa attacgatta tacattggga tgtctatata
     1321 gctttttctt atatgactgg aattattata catatccaca ccgagcgtat tcaaactaaa
     1381 aggagggaca gaaagagtat attctttact cactccctcc cccccctttt agctcaagcg
     1441 tcaactgcaa attagttata ctactaattt tatctgcatt aatttcaata ctgacattgc
     1501 atattttggg tttttcattt gcgttaaagc cataggtccc gtaatgtttt aacgtgatgt
     1561 aaggtaaagt atcaccctgt gtttctcggg ttttctgatc ttgattacaa cgggttaaat
     1621 attcatcctt attgaatttc attgaatatt tccccgcacc tttttcgtaa taatcccata
     1681 ccttatcaca atcaagcgtt tcacctaatc cgttgtaata catccaactt aaccacaatg
     1741 cggctttttt tgccccttgc tgttcagcct tgtcaaaaag catgctcgct ttagaataat
     1801 caacctcacc ggtcaaacca gaatagtata tattccccag ggccactaat gctttttcgt
     1861 cattcttatt tgcagctaat tcgtaccatt ttttggcttc gacagcatca atctttaaac
     1921 cagcactgcc attttcgtag gtatcgccca tggtatactg cgccatcgga taaccttttt
     1981 ctgccgcagt acgaaaccat tgcagtgctt tctttatggt gtctaaagaa cgtctatcat
     2041 cagaaggtga aaaatcatcg agataaagat ttccgacaat ataatccatt tcggggtcgc
     2101 cttcctgcgc ccatagagtc gccagacgca gaatttcagg tttactgaaa ccaattcgcc
     2161 cccaaaaaag caaatcattc tcgggtgcct gaccgacata acgactgaca aattcaatta
     2221 aactcgcccg tttcgttttg tctgtctcag ccccggattt caattcatag gcctgattga
     2281 ttattttatt catcaaatca ggcggaatat taagatcgtc agttttcgac ttactggcat
     2341 acttctgtgc tcgtgtcaca tccagggtga cgcctttacc atattgaaag aaataacgtg
     2401 cattataaca accgtattcg cccccctggt tacaggcttt ctcataccac tgaatcgcct
     2461 ttgggatgtc aacagatact gtcactccgt gttcataaat aaatcccagt agcactagcg
     2521 cctgcgtgtt attggtatca gctaattttt gcagttcttt taacgccgtc tgatctttat
     2581 tgacaatacg ttcatacagt tgatcaatat ctgttgaagc atgacaaaaa gaagccagag
     2641 ataaacccaa tgataataaa ataactttac cagaccacat acattcctcc ttgatatatt
     2701 caaccaccaa acatccacct catgaataaa tacgctgcca taacggccca ggtaataagt
     2761 aaacatttaa gctgaaacag cataaccagt gcctccaacg cttctattta ttgcgataaa
     2821 ataacaccat cctttgataa aaagtcacag cccatcaata aatccaaaaa taatgtcatt
     2881 agttgcagga taaaatcatt tgagcttttc atttgttata tttaacgcat gatgtattaa
     2941 tggcaagaat atgagagaaa ctcatacctg acgtaaaaat cactctctta tgtaatattt
     3001 acttattaac actctgttta cctaacatgc gctccgcact acactatgtg ggcaattttt
     3061 taagaggtgg taatgaacga ttataaaatg acgccaggtg agcggcgcgc gacctggggt
     3121 ttagggaccg tattttcatt gcgcatgctg ggcatgttta tggtactgcc ggttctgact
     3181 acgtacggta tggcactgca aggcgccagc gaagcattaa tcggtattgc cattggtatt
     3241 tatggtctga cccaggccgt ttttcagatt ccgtttggcc tgctttccga ccgcattggt
     3301 cgaaaaccat taattgtcgg tgggctggca gtgtttgccg ccggtagcgt tatcgctgcg
     3361 ctttctgact ccatctgggg aattattctg ggccgggcgc tacaaggctc cggtgcgatt
     3421 gccgctgccg ttatggcgct gctttccgat ctcacgcgcg aacaaaaccg taccaaagca
     3481 atggcgttta tcggcgtgag ctttggcatt acctttgcca ttgcgatggt gcttggcccg
     3541 atcatcactc acaaacttgg gctgcacgcg ctgttctgga tgatcgctat tctggcaacg
     3601 accggcattg cgttgaccat ttgggttgtg cccaacagta gcactcacgt acttaatcgt
     3661 gagtccggaa tggtgaaagg cagtttcagt aaagtgctgg cggaaccgcg gctgctgaaa
     3721 ctcaactttg gcattatgtg tctgcatatt ttgctgatgt cgacgtttgt tgccctgccc
     3781 ggacaactgg ctgatgcggg gttcccggcg gctgaacact ggaaggtcta tctggcgaca
     3841 atgctaatcg cctttggctc ggtcgtgcct ttcattatct acgctgaagt taagcgcaaa
     3901 atgaagcaag tctttgtctt ctgtgtcggg ttgatcgtgg ttgcggaaat tgtgttgtgg
     3961 aacgcgcaaa cgcagttctg gcaactggtg gtcggcgtgc agcttttctt tgtggcgttt
     4021 aatttgatgg aagccctgct gccctcactt atcagtaaag agtcgccagc aggttacaaa
     4081 ggtacggcga tgggtgttta ctccaccagc cagtttcttg gcgtggcgat tggcggttcg
     4141 ctgggcggct ggattgacgg catgtttgac ggtcagcggg tatttctcgc tggcgcaatg
     4201 ctggccgcag tgtggctggc agtcgccagt accatgaaag aaccgccgta tgtcagcagt
     4261 ttgcgcattg aaatcccaga aaacgtaatg gcagatgaaa aactggaaaa atgcttactg
     4321 acaacgcctg gtgtcaaaga tgtactgctg gcgaagttag aacattgcgt ctatgtgaaa
     4381 gtagacagta aaatcaccaa tcgttttgaa atagagcgag taatcaacga agtatcttat
     4441 taaataataa gcacggtgct attcatgaaa aataaaatag caccgtactt cgtaacacca
     4501 taatccttta aaccctccat tattaatact tcgcttaatt ccatataact ggacgattac
     4561 attagaagta aactatactt tttatgaaat atcagggata aattattcac atggaa
//
LOCUS       DADLSE010000229         4449 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLSE010000229 DADLSE010000000
VERSION     DADLSE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.230"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1229)
                     /locus_tag="JN692_003982"
     CDS             complement(<1..1229)
                     /locus_tag="JN692_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7266120.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNVGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRS"
     gene            1446..2369
                     /locus_tag="JN692_003983"
     CDS             1446..2369
                     /locus_tag="JN692_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7266121.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN692_003984"
     CDS             complement(2407..4047)
                     /gene="trg"
                     /locus_tag="JN692_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7266122.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRNRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
BASE COUNT         1075 a         1170 c         1045 g         1159 t
ORIGIN      
        1 tgactgcgtg tctgcgtttc attacccaga tttgactgga agatccccgc cgcgctaacg
       61 ggcaagaaat cttcataggt gatgggttgc gccactaccc aaccacgttc aattaagggc
      121 tgtggatcgt ctccaggatg aatcgcctga cgatgcgcct cacccgaagg cgtcagacgg
      181 taccggaacc atgccaaccc ttgctgacgc attaaaaact cactgtcagg aaaagtgcgg
      241 aaggtttcct gtaaatgcat ttggtgagtg agattatcct gcccggttcc aacgttacgc
      301 agaagatcat catacagttg tcgccctttc ggcgttaatg ccacgccacg ctgctcaatt
      361 tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg caaacaacac cgtctcttcc
      421 agtgctttaa agctggtctg ccgtagtaaa atcggtacct cgcggcgcgg cggcccctcg
      481 atgagaattt tgggttcaat tccgcattca ggcatcatcg actgcacccg gtcaatatcc
      541 agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa aacagaccac atcagcaatc
      601 aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat ctaccgttgc taactggtgc
      661 cagcgaaacg tttccagggc ttcctgcaca aactcctgtg cctgtgtttc gttaaaaccg
      721 ccctgctgct catattcctc taacagttgt cgacaacgtg gggtgaagat atcgcgctga
      781 cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct cgataagctc aaggcggagt
      841 aaggatgtaa aaacgcggaa gggattacgc gccagagaag catcatcaat gggccgaaat
      901 gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt aatagctcac cgggtacatc
      961 cccataatgg caaacatccg ccgaagagta gcaagctctt gtgcagtccc aacgcgaatc
     1021 gccccatgac gttcaacatt cagtcgcgcc agctcgtctg catttaccat tttttcgtgc
     1081 agttgaggat tgttttccag cacagccaga ttcacatcag ctaccagttc cagcagcgtg
     1141 ccatattgcg gaacttcttg ctggtacatg gctgacattg cctgcgaaaa ctgttcccga
     1201 atctcatccg ccgtgatgct gttcgccata gtgtctgcct ccagtggtca gtaatctgga
     1261 gtgtaggtaa ccgcattcac tctggcggga agaatttaca aactgtgatc tcgccgcgaa
     1321 aacatcaata ttatccattt tgctgtaaca taattgcttt aattgttaat aatattttgc
     1381 aatcaagtta tcataatcaa acaacttcac ttgtcagcga caccgcttcg tttttaacat
     1441 cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg tttgcgccac cttcatacat
     1501 tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcggc tgaaaccctt aatttgagtc
     1561 aacctgcgct ctctaataca ttgaatgaac tggagcagct gacgggcgct cgcttgtttg
     1621 agcgtggtcg tcagggggcg caacttacct tacccggcga acaattttta acgcatgcag
     1681 tcagagttct tgacgccatc aacactgccg gacagtcgct tcatcgtaaa gaaggtctta
     1741 ataatgatgt cgtcagggtt ggtgcactac ctactgcggc actggggata ttaccttcgg
     1801 ttataggtca gtttcatcag caacaaaaag agacgacctt gcaagttgcg acaatgagta
     1861 accctatgat tctggcgggt ttaaaaaccg gggaaatcga tatcggcatt ggtcggatgt
     1921 cagatcctga actgatgacc gggcttaatt acgaactgct gtttcttgaa tcgttgaagc
     1981 tggttgtccg ccctaatcac ccgctacttc aggagaacgt aacgctaagc cgggtgctgg
     2041 aatggccggt cgttgtatca ccagaaggca ctgcgccacg ccagcattca gatgcattag
     2101 tgcagagcca gggctgtaaa attccttcgg gttgtatcga aacgctgtct gcttcgctat
     2161 ctcgtcaact taccgttgaa tacgattacg tgtggtttgt cccttctggc gctgtaaaag
     2221 acgacctgcg tcatgccacg ctggtggccc tgcctgttcc gggacatggt gcaggcgaac
     2281 cgattggaat actgacccgc gtagatgcga cgttctcttc tggttgccag ttgatgatta
     2341 acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg atctgtcgat ccctccttga
     2401 acattttcac accgtagcga aactaactgg ttcacccgct ccgcgaggtt ctgccgacac
     2461 agaatgtttg ttcagacgga atacgttcac cgcctcagtt aatcgtgcag cctgttcttc
     2521 aagggacacc gccgctgctg aggcctcttc taccagagaa gcattctgtt gcgtcacctt
     2581 atccatttca gaaatcgcct ggctaacctg agttatgcct ctactttgtt catccgaggc
     2641 ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg gcatcaacaa tagtgctcat
     2701 ggttttccct gccgttgcca cctcatccga ccccaggtca attaacctga ctgattcact
     2761 gatcaagcct tcaatctctt tcgctgcctg agcgctgcgg cttgcgagtg tccgtacttc
     2821 gctggcgaca acggcaaatc cacgcccttg ctcacccgct cgcgcggctt caacggcagc
     2881 attcagtgcc agaatattcg tctggaaagc aatactgttg atgacggcgg tgatctcaga
     2941 aattttcttc gaactcgtgg agatagcgcc catcgttttt actacaccgg aaaccgtctg
     3001 cccgccatcg ctggctttaa tagaagcctc ttgcgccagt ttgctggcat gatgcgcgtt
     3061 atccgcattc tgtttcaccg tcgcagtgag ttgctccatg ctggcggcag tttgttcgat
     3121 agccgccgct tgttcttcgg tgcgagatga cagatccgca ttgccagctg aaatttcgct
     3181 ggtgccacga taaatctctt ccgcgccctg tcgaacagtc cctactgtca tccccagtga
     3241 atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt tcattacgac ccgccggttc
     3301 atcattcatc gtcagatcgc cactggcgat tttttcaatc cgttgtgcgg catgttgcag
     3361 tggacgaatg acgatccgac gtagcaccat aaatgttatc agcgtcatga ccagggcaag
     3421 cacaaacgcg ccaatcatga acatcccacc caggcggttg cgctgatggg ccagttccgc
     3481 cagttggttg gctctggtgc tacgtatctt aacggctttg ttcaaaatat cggtataagc
     3541 attatccagc ggtcggatct gctcactttc atgattgata atcgcttcaa acatgccatt
     3601 tttggcatat ttcaacatag gttgcatacc cgtgatataa gcctgaaagc gttgatttaa
     3661 ttcagtgtcg agggcttcat cagcaggtgt tttcaccggt cgattctgat aagcacgata
     3721 accttgctgc gactgtttaa tctccgattc ggcttgcgca atatttcgct tcattgcttc
     3781 catttccgca atacgactcg cagccccggc ctgaatcata ttgatccggg ctgaacgcaa
     3841 aaaatctgaa ctgttcgcta acccggtccg aatctcaatc tccgcagtaa catcacgctg
     3901 atcgcgatcg gcctgccaga ggaaatagcc agccagagca gaacttaatg cgaatagaac
     3961 taagataccg ccaagaatgc aggcaaataa cggaaccagc ctgatgtgat gcaaaaaacc
     4021 taatcgctgt gagggagttg tattcattac cgttgtctct cgtccaggtt tactcctgat
     4081 agaaagtcat cggcacttga cggtaattac ttatgaccaa aaacctcttt gcgtcacatt
     4141 tttacaacaa ttattgcaga gaaaattaat gctattggaa tgaagagagt aacgaatggc
     4201 taaactggct aacccgaatg acgaaaaatt ctggaaaaca gcacgcaatt tcgtgaagtg
     4261 attattttcg tccctaagga aacctcatag gatgcctgcg ggtgcctgag actttctgtt
     4321 ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc gatatttcta tcaactgtac
     4381 tgcacccatt ttgttggacg atgaaatgga atagccccta atatgtcaaa gccaaaatac
     4441 ccttttgaa
//
LOCUS       DADLSE010000230         4356 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLSE010000230 DADLSE010000000
VERSION     DADLSE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.232"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            105..257
                     /gene="hokA"
                     /locus_tag="JN692_003985"
     CDS             105..257
                     /gene="hokA"
                     /locus_tag="JN692_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7266123.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN692_003986"
     CDS             complement(444..656)
                     /gene="cspA"
                     /locus_tag="JN692_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7266124.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(937..1227)
                     /locus_tag="JN692_003987"
     CDS             complement(937..1227)
                     /locus_tag="JN692_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7266125.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1661..2371
                     /locus_tag="JN692_003988"
     CDS             1661..2371
                     /locus_tag="JN692_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7266126.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN692_003989"
     CDS             complement(2421..3395)
                     /gene="ghrB"
                     /locus_tag="JN692_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7266127.1"
                     /translation="MKPSVILYKALPDNLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3499..4158)
                     /locus_tag="JN692_003990"
     CDS             complement(3499..4158)
                     /locus_tag="JN692_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7266128.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1071 a         1051 c         1059 g         1175 t
ORIGIN      
        1 taactgtcca actttttggg gtcagtacaa agtgaggcta ccctatgcct gaacaccata
       61 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
      121 gattactttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
      181 attcactgtg tgaattgcat attaaacagg agagttatga gctggcggcg tttttagcct
      241 gcaaattgaa agagtaagag tcttcggcgg gaaattattc ccgccttact tacggcgttg
      301 cgcattctca ttgcacccac atttattctt cacaaaaata ataatagatt ttattacgcg
      361 atcgattatt tatttcctga aaaaaaataa aaaaatcccc gccaaatggc agggatctta
      421 gattctgtgc ttttaagcag agattacagg ctggttacgt taccagctgc cgggccttta
      481 gcgccgcttt cgatggtgaa ggacactttc tgaccttcgt ccagagattt gtaaccatcg
      541 ttctggatag cagagaagtg tacgaacaca tctttagagc catcgtcagg agtgatgaag
      601 ccgaagcctt tgtcagcgtt gaaccatttt acgataccag tcattttacc ggacatagtg
      661 tattaccttt aataattaag tgtgcctttc ggcgatatgg cgtgctttac agattttgaa
      721 gcgttaaagg aatgtgcact acgaggggta tcaacgataa ctcttgaagg gacttgcctt
      781 actacactgc tttaatggtc tgtacgtcaa accgttgatg tgcattaagc cacgcattgg
      841 cgggtgatgc aacaattatt tttcatattt atgattaatc ggttggaaaa cggtcctgtc
      901 atcaggaccg taaacagcaa taaagtggat aaaagtctat ttcatcaact gcttacttaa
      961 tgccgggttg gcttgaatca aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg
     1021 tctggattcc cattccttta ccatggcgac tgatacgccc aaaacccggg cgaaatcatc
     1081 gatttttaat cctgtccctt ttcgtaactg ctcaatttcg gtacaggacg ttgttctgtg
     1141 cgtcagggta attttctgcg tttcatcttt aaaaacaatc tgttccaggc tgctcaacag
     1201 ctcatgcatt ggatctttat attccattga aaactcctca aatcacactg cgggatcgtg
     1261 aattgcatcg aagctcatta agaatagtcg ggaaaagcag acacagcggg gtgaagtcag
     1321 ggcatgctct ggctatgatt cgattcagcc tgtcatttag cgtgacttat agccacctaa
     1381 cgatctgata aatgccgata tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc
     1441 taaccttctg cgaaggcgta aatctctggc tacaaaaaag gcaacggtac tatcattttt
     1501 taacaatgaa acaacaaatt aaccttgttt cattttctcc atgattttac caggaagatt
     1561 acgtagaaag ggtatcctgc aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt
     1621 gtgtcgttgt gcgctttttt tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg
     1681 ctctcgctgg tttgcgcctc ttgcggcgtt attaatggta gttagcctga gtgggtgttt
     1741 tgataaagaa ggcgatcagc gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg
     1801 tagcggtgaa cgtctaccaa ccctgactgc cgatcagaaa aaacagtttg gtccttttgt
     1861 ctctgattac gcgattctgt atggttattc tcagcaggtg aatcaggcga tggattccgg
     1921 tctgcgcccg gtcgtagaca gcgttaacgc aattcgcgta ccgcaggatt acgttacgca
     1981 aagcggtccg ctgcgtgaaa tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa
     2041 tgcgaagttg caggccgatg ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc
     2101 ggtatttgat caggcattca ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt
     2161 gattccggcg gcacaaactt tcacgcaaca actggtgatg gttggagact acattgctca
     2221 gcaaggtact caggtgagct ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc
     2281 aagtgaatat aacaaactga ttgcgccatt accggcacaa catcaggcgt ttaatcaggc
     2341 ctggactaca gcagttactg caacccaata aagagtaaaa gcccgagcga ttatcgggct
     2401 ttacttcacg cagtcgcggc ttagtccgcg acgtgaggat tcacacagtt cttctcaacc
     2461 tttccttgta acgcatcaat caaattatcc acggcacagg cggccatgcc ataacgcgtc
     2521 tcatgggtgg cagatccaat atgcggtact gcgacgacgt tggccattga gagcaacggc
     2581 gaatctacgg acagtggctc ttgttcgaag acatccagcc cggcggcgtg aatttcgcct
     2641 ttctgcaatg ctgcgatcag tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg
     2701 aaaatggcgg aggatttcat tttggcgaat tgttctgcgc caaacagatg atgcgtctca
     2761 tcagttaacg gcaggatcag gcaaacgaaa tctgactctt gtaacagagt atccaaatcg
     2821 cagtagcggg cgttgaagcg ttcttctgct tctttatggt ggcggcgcgc gttatagagg
     2881 atgggcatgt tgaagccaaa gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc
     2941 ccgacaatgc ccagtgtttt atggtgaacg tcagtgccgt accagtccgg gcctatgctc
     3001 gcggtccatt cgcctgcttt tacccgttct gctacctcca caacccgacg agcggtagac
     3061 aacaccagcg ccatcagcgt atcggcgacg gtttctgtta atacggttgg cgtgtgcatc
     3121 agcagaattt ttcgggcggt aagcgcatcg acatcaaaat tgtcatagcc gacggagatc
     3181 gttgatgtgg cacgcagttt cggcattttt tccagcaatg cggcatctac attctcgttt
     3241 gaacccagta aaccttcagc ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg
     3301 ctgaggtttg ccacctggtg aacggtgaaa tgcgcttgca ggcgttgcag taaattatca
     3361 ggtaaggctt tgtagaggat aacggacggc ttcatgcttc tctccattca ctgataaggc
     3421 gatcacctta gcctgaaata tgatgaagga aaactgaaga atgccaggtg atttctgcat
     3481 cacctggcat gaaagggatt acagcgggct taaggtaatt tctacacggc ggttttgcgc
     3541 cttaccttct gcggtgctgt tgctggcgat tgggttagcc gggccaaggc cctgagtacg
     3601 gatgcggctg gcgtccacgc cctgggtgat caacgcgctg gcaacggaat ccgcacgctg
     3661 ctgggagaga cgcatgttca ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt
     3721 aaccgccgtt ttcggatact ctttcagcac cattgccacg ccggtcaggg tgttagcgcc
     3781 tgccggtttc aggggcgcgc tgctgctgtc gaaggtcaca ttgttcggca tattgaggat
     3841 aatgttatcc ccgctgcggg ttacgctaac accagtgccg cgcattttgt cacgcagctt
     3901 cgcttcctgc acatccatgt aataacccac gccgccgccc agagctgcgc ctgctgctgc
     3961 gccaatcagc gcgcctttac cgcgatcttt cttcgaagaa gagagcgcac caatacccgc
     4021 gcccacgaga gagcccagac ctgcgccgat agcagatttg cctgcttcgc gttcgccggt
     4081 gtaagggtta gttgtgcagc cagataccgc cagagcgcca ctcactacgg cggcaataag
     4141 ataaacacgt ttcttcattg ttaatcctta ataacctttt tattctttgc cacgggttcc
     4201 gtggcgggag attatgccgc gtgaacatga agattattcc tgggaatact cggaaatttg
     4261 taagtaatat ttaactgctc aatacatcta acctttcagg agtcttcggt ttggccaact
     4321 catcctcacg atattccgtt ctgactgccg cccact
//
LOCUS       DADLSE010000231         4324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLSE010000231 DADLSE010000000
VERSION     DADLSE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.233"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            176..1459
                     /locus_tag="JN692_003991"
     CDS             176..1459
                     /locus_tag="JN692_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HAY7266129.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            1606..3081
                     /locus_tag="JN692_003992"
     CDS             1606..3081
                     /locus_tag="JN692_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7266130.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            3360..>4324
                     /locus_tag="JN692_003993"
     CDS             3360..>4324
                     /locus_tag="JN692_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7266131.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVWMNFRLYQNVSRENISDAMTGLYNR"
BASE COUNT         1170 a          974 c          938 g         1242 t
ORIGIN      
        1 tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct
       61 taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcct
      121 gagaagcggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac
      181 ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt
      241 tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc
      301 ggtgactgac gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc
      361 aatgtttcat caggggcgtg gcggtctgcg ccaccgcgtg catccgggca atgaccattt
      421 cgttcagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggtca
      481 gggccaggcc agccaggatg gtgaaggtca ggatgaattt gtctttcaga tttcgaaaga
      541 tgagtatctt gatctgctct ttgaagattt ggccttaccg aatctgaaac aaaaccaaca
      601 acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccagc
      661 caatatcagc gttgtgcgtt ccttgcagaa ctcactggcg cgacgcacag ccatgacggc
      721 aggcaagcgg cgggaacttc atgcactgga agagaatttg gccatcatca gcaacagtga
      781 acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa
      841 aattgaacgc gtccctttta ttgacacctt cgatttacgt tacaagaact acgagaagcg
      901 gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga
      961 tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag
     1021 aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga
     1081 tgaacatgag tttttctact cgcaggaaac aggcggcacc attgtttcca gcgccctgaa
     1141 actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc
     1201 acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc
     1261 gaaaaaatta ttacctgttg ttcgttatta cagctatatc gaaattaccc gtcgtgcaca
     1321 tcagacattg tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca
     1381 gcacatccgc gaccaggatg atatttatcc ggtgttccgt gaactgtttc ataaacaaaa
     1441 tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct
     1501 tactgtaaat tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc
     1561 ataaccttta cacaatttag ttccagaaaa caatcattcg gaaaaatgat tcagtcaaca
     1621 cgtatttccc tggggttatt ctttaaatat tttttatcgt taacgaaaat tgatcttggt
     1681 caaaactata tatctctgcc atcaataaaa tccagcactc acattgctct cctttttatg
     1741 gtttctatgg gtacacaaaa attaaaagct caaagctttt ttattttcag tttattgctg
     1801 acgttaattt tattttgcat caccacatta tataacgaaa agacgaatgt aaaactcatc
     1861 ccacaaatga attatctgat ggtcgttgtg gctttgtttt tccttaacgc catcattttt
     1921 cttttcatgt taatgaaata tttcactaac aaacaaattc taccaacact cattttaagt
     1981 cttgcatttt taagtggcct tatctattta gttgaaacta ttgttgttat ccataaacct
     2041 atcagcgaca gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc
     2101 cgccaactca gttttatttg tttaacctcg ttggcgctct tttgttatgg taaagacaac
     2161 atccttgaca acaataagaa aaaaactggc attctgttgc tggcgctgat tcctttttta
     2221 gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc
     2281 gtcgattact gtcctgacaa ccatactgcg acctggggaa tcaactatac aaaaatattg
     2341 gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa
     2401 ctatggccat taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg
     2461 actctggatg agtataatta caccatctgg tacatcagtc gcgggattga agtctccagt
     2521 aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc
     2581 aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc
     2641 gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt
     2701 aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt
     2761 tcaattgtcg atattatcca gcaaagcatc cgccccgatg atattttagc gcgactggag
     2821 ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg
     2881 gaacgtatgc gtaaaaatgt cgaactcctg accggcttca gtaacagata tgatgttcct
     2941 gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg
     3001 cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa
     3061 gtgattattc ataatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt
     3121 tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt
     3181 ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta
     3241 accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc
     3301 gcaagtgtca ttgtgctgac atcttcctta tttttgaact tgtcgccagc gacagagcaa
     3361 tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt tatgataagt
     3421 atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag cagtcggaag
     3481 tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat aatacccatg
     3541 gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa accgcatcca
     3601 ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat caggcagtgg
     3661 aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa gagaaatttc
     3721 actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt aatgttgaat
     3781 actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc gatcgcaaag
     3841 atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag gatcagcgta
     3901 ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag ctaaaaggga
     3961 ttgtgctgct ggatattaac aaaaacaatt tgcggaatat cttttacact catgaccgcc
     4021 ctctcctctg gcgttttctc aatgtcacgc taaccgatac cgattcgggg cgcgacatta
     4081 tcatcaacca gagcgaagat aatctgttcc agtatgtcag ttacgtccat gacttaccgg
     4141 gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct tcgtggaaaa
     4201 gcgttctgtt ctggattttg acggcgttaa ttttgctgaa tatggtctgg atgaacttcc
     4261 gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga ctgtataatc
     4321 gcaa
//
LOCUS       DADLSE010000232         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLSE010000232 DADLSE010000000
VERSION     DADLSE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.234"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..767
                     /gene="cheR"
                     /locus_tag="JN692_003994"
     CDS             <1..767
                     /gene="cheR"
                     /locus_tag="JN692_003994"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HAY7266132.1"
                     /translation="LIYQRAGIVLADHKRDMVYNRLVRRLRSLGLTDFGHYLNLLESN
                     QHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYRVWSAAASTGEEPYSIA
                     MTLADTLGTVPGRWKVFASDIDTEVLEKARSGIYRHEELKNLTPQQLQRYFMRGTGPH
                     EGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMIYFDQTTQQEILRRFVP
                     LLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            770..1819
                     /locus_tag="JN692_003995"
     CDS             770..1819
                     /locus_tag="JN692_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HAY7266133.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            1834..2223
                     /gene="cheY"
                     /locus_tag="JN692_003996"
     CDS             1834..2223
                     /gene="cheY"
                     /locus_tag="JN692_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HAY7266134.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDSLN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            2234..2878
                     /gene="cheZ"
                     /locus_tag="JN692_003997"
     CDS             2234..2878
                     /gene="cheZ"
                     /locus_tag="JN692_003997"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HAY7266135.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQASERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(3335..3718)
                     /locus_tag="JN692_003998"
                     /pseudo
     CDS             complement(3335..3718)
                     /locus_tag="JN692_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3687..>4313)
                     /locus_tag="JN692_003999"
     CDS             complement(3687..>4313)
                     /locus_tag="JN692_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HAY7266136.1"
                     /translation="EAKNDTWKEQRNALSPAEINELSMQMFRVYATSALINAVVEAFC
                     EQLPALMQRDCPFKDLISQSKGHALCTALKMFDRTRGYLHRSVLQQELRGSNYITNLM
                     DMLWIGIHGHKTKKQRDGERESASADNLFVSREEYLSDTPFGRYAYGTISENYRRVFE
                     DPANTLPVLYKEAQLLTDAISGMTDSYLMRLHDELKSLYECGLKHPTA"
BASE COUNT         1082 a          981 c         1183 g         1067 t
ORIGIN      
        1 aattgatcta tcaacgagcc gggatcgttc tggctgacca taaacgcgac atggtttaca
       61 accgactggt tcgtcgtttg cgttcgctgg gactgacgga tttcggtcat tatctgaact
      121 tgctggaatc taatcagcac agcggtgagt ggcaggcgtt tatcaattcg ctgaccacga
      181 atctgacggc atttttccgt gaggctcatc atttccctct gctcgcggat cacgcacgtc
      241 gccgttctgg cgagtatcgc gtatggagcg cggcggcttc gaccggcgaa gagccgtaca
      301 gcattgcgat gactctggct gacacattgg gcaccgtgcc cggacgctgg aaagtgtttg
      361 ccagtgatat cgacaccgaa gtgctggaaa aagccagaag cggtatctat cgccatgaag
      421 agttgaaaaa cctgacgccg cagcaactgc aacggtattt catgcgaggg acgggaccgc
      481 atgaagggct ggtacgcgtg cgtcaggagc tggcgaacta tgttgatttt gccccgctga
      541 atttactggc gaaacagtac accgtgccgg ggccgtttga tgcgatcttc tgtcgtaacg
      601 tcatgatcta cttcgatcaa actacccagc aggagatttt gcgccgcttt gttccgctcc
      661 ttaaacccga cggattgctg tttgcgggtc actctgaaaa ctttagccac ctcgagcgcc
      721 gcttcacgct gcgtggtcag acggtgtatg cgctaagtaa ggattaacga tgagcaaaat
      781 cagggtgtta tctgtcgatg attcggcact gatgcgccag atcatgacag aaatcatcaa
      841 cagccatagc gacatggaaa tggtggcgac cgcgcctgat ccgctggttg cgcgtgactt
      901 gattaagaaa ttcaatcccg atgtgctgac gctggatgtt gaaatgccgc ggatggacgg
      961 actggatttc ctcgaaaaat taatgcgttt gcgtccaatg cccgttgtga tggtttcttc
     1021 cttgaccggc aaaggatcag aagtcacgct gcgcgcgctg gagctggggg caatagattt
     1081 tgtcaccaaa ccgcaactgg gtattcgcga aggaatgctg gcgtatagcg aaatgattgc
     1141 tgaaaaggtg cgtacggcag caaaggcgag ccttgcagca cataagccat tgtcagcacc
     1201 gacaacgctg aaggcagggc cgttgttgag ttctgaaaaa ctgattgcga ttggtgcttc
     1261 aacgggtgga actgaggcaa ttcgtcacgt actgcaaccg ttgccgcttt ccagcccggc
     1321 attgttaatt acccaacata tgccgcccgg tttcacccgc tcttttgccg acagacttaa
     1381 taagctttgc cagatcgggg ttaaagaagc cgaagacgga gaacgtgtct tgccggggca
     1441 tgcctatatt gcgccgggcg atcggcatat ggaactggcg cgtagtggcg caaattatca
     1501 aatcaaaatt cacgacggcc cggcggttaa ccgtcatcgg ccttcggtag atgtgttgtt
     1561 ccattctgtc gccaaacagg cggggcgtaa tgcggttggg gtgatcctga ccggtatggg
     1621 caacgacggc gcggcgggaa tgttggcgat gcgtcaggcg ggggcatgga cccttgcgca
     1681 aaacgaagca agttgcgtgg tgttcggcat gccgcgcgag gccatcaata tgggtggtgt
     1741 ctgcgaagtg gtcgatctta gccaggtaag ccagcaaatg ttggcaacaa ttagtgccgg
     1801 acaggcgata cgtatttaaa tcaggagtgt gaaatggcgg ataaagaact taaatttttg
     1861 gttgtggatg acttttccac catgcgacgc atagtgcgta acctgctgaa agagctggga
     1921 ttcaataatg ttgaggaagc ggaagatggt gtcgactctc tcaataagtt gcaggcaggc
     1981 ggttatggat ttgttatctc cgactggaac atgcccaata tggatggcct ggaattgctg
     2041 aaaacaattc gtgcggatgg cgcgatgtcg gcattgccag tgttaatggt gactgcagaa
     2101 gcgaagaaag agaacatcat tgctgcggcg caagcggggg ccagtggcta tgtggtgaag
     2161 ccatttaccg ccgcgacgct ggaggaaaaa ctcaacaaaa tctttgagaa actgggcatg
     2221 tgaggatgcg actatgatgc aaccatcaat caaacctgct gacgagcatt cagctggcga
     2281 tatcattgcg cgcatcggca gcctgacgcg tatgctgcgc gacagtttgc gggaactggg
     2341 gctggatcag gccattgccg aagcggcgga agccatcccc gatgcgcgcg atcgtttgta
     2401 ctatgttgtg cagatgaccg cccaggcttc ggagcgggcg ctgaacagtg ttgaggcgtc
     2461 acaaccgcat caggatcaaa tggagaaatc agcaaaagcg ttaacccaac gttgggatga
     2521 ctggtttgcc gatccgattg accttgccga cgcccgtgaa ctggtaacag atacacgaca
     2581 atttctggca gatgtacccg cgcataccag ctttactaac gcgcaactgc tggaaatcat
     2641 gatggcgcag gattttcagg atctcaccgg gcaggttatt aagcggatga tggatgtcat
     2701 tcaggagatc gaacgccagt tgctgatggt gctgttggaa aacatcccgg aacaggagtc
     2761 gcgtccaaaa cgtgaaaacc agagtttgct taatggacct caggtcgata ccagcaaagc
     2821 cggcgtggta gccagtcagg atcaggtgga cgatttgttg gatagtcttg gattttgatt
     2881 tgtattgcct gaacttcggc ctgcgcgatg atgccaggcc gatagcaggg tggcgggtat
     2941 cgttcaggga gtacgtggag gatggttatt tgtagtttga taattttaga tcagcgaagc
     3001 gtgattgttg cgccagtaaa tttatcaatg cattaaattg tgaacgagaa aatgacgatg
     3061 actggtagtg cagtacatcg tagtgatgcc atacttcata acgttttttc gtcagttcat
     3121 ggtgcaagaa atgacagagt tccggcccta aactctttac ctgcttagta aaaatagtgc
     3181 gtaaaatctt cgttaccgaa ccgcttggat tggggttagt atgaagaaca agttcaagat
     3241 gtctgcgatt taagtcggtc aagtaattat ctttactgat aattttttca ctacgtagat
     3301 catagagcac ggcatcccaa ctcattagcg tagtgtttaa cagacttgat tcatcctcac
     3361 aaaatataag tttttcccgg aatgcccatg tctcattgag gttccagata tcaaattcga
     3421 agtcgtgata acgaaaacgg atcccaccaa acttattaaa acgcagttgt ttgacgggca
     3481 gttcagccaa tgtctggaag agttcctcac gggagcgacc gatgacgata tcaaggtctg
     3541 aagagaatcc atcgacgcca tacaagccta agtcacgcac catcccgcca aaaatccagg
     3601 ccggacagga acaggccaga gtatcaagta atactctggc tttcttatgc tcatcactaa
     3661 gcgtaaaaaa atcggcgata cgctgttcaa gctgttgggt gtttgagacc acattcatac
     3721 agtgatttta gctcatcgtg aaggcgcatt aaataactat ccgtcatgcc tgagattgcg
     3781 tcggtaagca actgcgcttc tttataaaga acagggagtg tattggcggg atcttcaaac
     3841 acgcggcgat aattttcaga tattgtcccg taagcatagc gaccaaaggg agtatccgac
     3901 aggtattctt cacgagaaac aaaaaggtta tctgcagacg cggactcgcg ttctccgtct
     3961 cgttgtttct ttgttttgtg tccatgaatg ccaatccaga gcatatccat caagttagta
     4021 atgtaattag accccctaag ttcctgttgc aagactgaac gatgaaggta gcctcgtgtt
     4081 cggtcgaaca ttttcagggc agtgcagagg gcgtgacctt tgctttgcga gattaaatct
     4141 ttgaacgggc agtcacgctg catcagcgcg ggtaattgtt cgcaaaaggc ttctactacg
     4201 gcattaatca atgcactggt ggcatacact ctgaacattt gcatactgag ttcattaatt
     4261 tccgccggtg aaagtgcatt tctttgctct ttccaggtgt catttttggc ctc
//
LOCUS       DADLSE010000233         4313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLSE010000233 DADLSE010000000
VERSION     DADLSE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.235"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..532)
                     /locus_tag="JN692_004000"
     CDS             complement(<1..532)
                     /locus_tag="JN692_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266137.1"
                     /translation="MTTVQFLYLLIIERDLQPTDWCSFMEKDYLRISSTVLVSLLFGL
                     ALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILS
                     LAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVS
                     ENTKQRNLFSKKMTLCI"
     gene            1047..3470
                     /gene="pgaA"
                     /locus_tag="JN692_004001"
                     /pseudo
     CDS             1047..3470
                     /gene="pgaA"
                     /locus_tag="JN692_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN692_004002"
     CDS             3479..>4313
                     /gene="pgaB"
                     /locus_tag="JN692_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HAY7266138.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPVDEQVKFGDEMVD
                     REYFATWQQVREVARSRLVEVASHTWNSHYGIQANSTGSLMPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GL"
BASE COUNT         1271 a          950 c          946 g         1146 t
ORIGIN      
        1 ttatgcacaa agtcattttt ttggaaaata aattgcgctg ttttgtattt tcactaactt
       61 tattcaccaa tgccagaaat attaatatgc acaaactcat ctgacgaaac aaataataga
      121 ttgcaatgtc attttggaca acactgcttg tcaaacgccc ctcaataatt tgctgaatga
      181 taatgacggt tttaacaaag taaatcaaac aactcagaaa agccaaacta agtatacagt
      241 ttgaaaattg cctacggtca taaatgaaat acaactgcat aaaaataaat gcaacagtat
      301 cgataaaaaa taaagcaatc attaccatca gataggttga tcgtggaaca acttcctcaa
      361 cgcctggctg attaaaccaa ctattcacca aaaccaaagc aagcccaaac aataagctca
      421 ctaatacagt actactaatt ctcaaatagt ctttttccat aaagctacac caatctgtag
      481 gttgtagatc cctttctatt attaatagat ataagaactg tactgttgtc aatatgaaat
      541 tccgcccaca aacaataccg atgatgatag ttgctgaatg taattattat tgcctaagtg
      601 ctttatacat tgtttgcaac aagccaagct tgaaaatact tataaatggt tatagaaaat
      661 ccttataaaa cattaattca tagatacatt aggaataaca attaaatccg tgagtgccgt
      721 agcgcagcct ttcatcagga ctttcgaaaa atccgaaatc atgcatcgga atttactgat
      781 ttaattattt taatcctaat ttattttgaa aaaggcattg ggatttatgc cgtattcctg
      841 aagatcctca tcattggaat ggattttcgg gcgagaaaag gattttatat ggacactctg
      901 ctcatcattt cttcttctca tcatcaacaa ttcacgtctc tcttccgcgt ttaataacgg
      961 attatgaggt gcaaaaatat ctttcttttc agttacctgt aattagatac agagagagat
     1021 tttggcaata catggagtaa tacaggatgt attcaagtag cagaaaaagg tgcccgaaaa
     1081 ccaaatgggc tttgaaactt cttactgccg catttttagc agcgagtccc gcggcgaaga
     1141 gtgctgttaa taacgcctat gatgcattga ttattgaagc tcgcaagggt aatactcagc
     1201 cagctttgtc atggtttgca ctaaaatcag cactcagcaa taaccaaatt gctgactggt
     1261 tacagattgc cttatgggcc gggcaagata aacaggttat taccgtttac aaccgctacc
     1321 gtcctcagca attaccagcg cgtggttatg cagctgtcgc cgtcgcttat cgtaacctgc
     1381 aacaatggca aaactcgctt acactgtggc aaaaggcgct ctctctggag ccgcaaaata
     1441 aggattatca acggggacaa attttaaccc tggcagatgc gggtcactat gatactgcgc
     1501 tggttaaact taagcagctt aactctggag caccggacaa agccaattta ctcgcagaag
     1561 cctatatcta taaactggcg gggcgtcatc aggatgaatt acgggcgatg acagagtcat
     1621 tacctgaaaa tgcatctacg caacaatatc ccacagaata cgtgcaggca ttacgtaata
     1681 atcaacttgc tgccgcgatt gacgatgcca atttaacgcc agatattcgc gctgatattc
     1741 atgccgaact ggtcagactg tcgtttatgc ctacgcgcag tgaaagtgaa cgttatgcca
     1801 ttgccgatcg cgccctcgcc caatacgctg cattagaaat tctgtggcac gataacccag
     1861 accgcactgc ccagtaccag cgtattcagg ttgatcatct tggcgcgtta ttaactcgcg
     1921 atcgttataa agacgttatt tctcactatc agcgattaaa aaagacgggg caaattattc
     1981 cgccctaggg gcaatattgg gttgcatcgg cttatctcaa agatcatcag ccgaaaaaag
     2041 cacagtcaat aatgaccgag ctcttttatc acaaggagac cattgccccg gatttatccg
     2101 atgaagaact tgcggatctc ttttacagcc acctggagag tgaaaattat ccgggcgcgc
     2161 taactgtcac ccaacatacc attaatactt cgccgccttt ccttcggtta atgggcacgc
     2221 ctacgagcat cccgaatgat acctggttac aggggcattc gtttctctca accgtagcaa
     2281 aatatagtaa tgatcttcct caggctgaaa tgacagccag agagcttgct tataacgcac
     2341 caggaaatca gggactgcgc attgattacg cgagtgtgtt acaagcccgc ggttggcctc
     2401 gtgcagcaga aaatgaatta aaaaaagcag aagtgatcga gccacgtaat attaatctgg
     2461 aggttgaaca agcctagaca gcattaacgt tacaagaatg gcagcaggca gctgtcttaa
     2521 cgcacgatgt tgtcgaacgt gaaccgcaag atcccggcgt tgtacgttta aaacgtgcgg
     2581 ttgatgtaca taatcttgca gagcttcgta tagctggttc aacaggaatt gatgccgaag
     2641 gcccggacag tggtaaacat gatgtcgact taaccaccat cgtttattca ccaccgctga
     2701 aggataactg gcgcggtttt gctggattcg gttatgccga tggacaattt agcgaaggaa
     2761 aagggattgt tcgcgactgg ctggcgggtg ttgagtggcg gtcacgtaat atctggctcg
     2821 aggcggagta cgctgaacgc tttttcaatc atgagcataa acccggcgcg cgcctgtctg
     2881 gctggtatga ttttaatgat aactggcgta ttggttcgca actggaacgc ctctctcacc
     2941 gcgttccatt acgggcaatg aaaaatggtg ttacaggcaa cagtgctcag gcttatgttc
     3001 gctggtatca aaatgagcgg cgtaagtacg gtgtctcctg tgctttcact gatttttccg
     3061 acagtaacca gcgtcatgaa gtctcaattg agggtcagga acgcatctgg tcttcaccat
     3121 atttgattgt cgatttccta cccagtctgt attacgaaca aaatacagaa cacgataccc
     3181 catactacaa ccctataaaa acgttcgata ttgttccggc atttgaggca agtcatttgt
     3241 tatggcgaag ctatgaaaat agctgggagc aaatcttcag cgcaggtgtt ggtgcctcct
     3301 ggcaaaaaca ttatggcacg gatgtcgtca cccaactcgg ctacgggcaa cgcattagct
     3361 ggaatgacgt gattgatgct ggcgcaacgc tacgctggga aaaacgacct tatgacggtg
     3421 acagagaaca caacttatac gttgaattcg atatgacatt cagattttaa ggataaatat
     3481 gttacgtaat ggaaataaat atctcctgat gctggtgagt ataattatgc tcaccgcgtg
     3541 catcagccag tcaagaacat catttatacc gccacaggat cgcgaatctt tactcgccga
     3601 gcaaccgtgg ccgcataatg gttttgtggc gatttcatgg cataacgttg aagacgaagc
     3661 tgccgaccag cgttttatgt cagtgcggac atcagcactg cgtgaacaat ttgcctggct
     3721 gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt cgtgaagcac atcgaggagg
     3781 aaaaccgcta ccggaaaaag cagtagtgct gacttttgat gacggctacc agagttttta
     3841 tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct gctgtatggg cccccgtcgg
     3901 cagttgggtc gatacgccag tggatgaaca agtaaagttt ggcgatgaga tggtcgatcg
     3961 agaatatttt gccacgtggc aacaagtgcg agaagttgcg cgttcccggc tcgttgaggt
     4021 cgcttctcat acatggaatt ctcactacgg tattcaggct aattccaccg gcagcttaat
     4081 gcctgtatat gtaaatcgtg catattttac tgaccacgca cggtatgaaa ccgcagcaga
     4141 ataccgggaa agaattcgtc tggatgctgt aaaaatgacg gaatacctgc gtacaaaggc
     4201 tgaggtaaat ccacacgttt ttgtttggcc ttatggcgaa gcgaatggca tagcgataga
     4261 ggaattaaaa aaactcggtt atgacatgtt tttcaccctt gaatcaggtt tgg
//
LOCUS       DADLSE010000234         4259 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLSE010000234 DADLSE010000000
VERSION     DADLSE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.236"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..829
                     /locus_tag="JN692_004003"
     CDS             <1..829
                     /locus_tag="JN692_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7266139.1"
                     /translation="PTQLLNFDPSTMQWATSSPVPSTFIGALIISIVAGYLVKWMNQK
                     IQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITPFGGWINGGIRTVLTAAGEKGALMY
                     AMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLPVTARSIAIVIPPIGLGLATIIDRR
                     LTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFALESPITAIPSYMVGAIVGSTAAVW
                     LGAVQWFPESAIWAWPLVTNLGVYMAGIALGAIITALMVVFLRLMMFRKGKLLIDSL"
     gene            844..1927
                     /gene="ypdF"
                     /locus_tag="JN692_004004"
                     /pseudo
     CDS             844..1927
                     /gene="ypdF"
                     /locus_tag="JN692_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            1927..2963
                     /gene="ypdE"
                     /locus_tag="JN692_004005"
                     /pseudo
     CDS             1927..2963
                     /gene="ypdE"
                     /locus_tag="JN692_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            2988..>4259
                     /locus_tag="JN692_004006"
     CDS             2988..>4259
                     /locus_tag="JN692_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7266140.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQSTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGVPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE"
BASE COUNT          911 a         1136 c         1263 g          949 t
ORIGIN      
        1 gccgacgcaa ctgctgaact tcgatcccag cactatgcaa tgggcgacct cttcgccggt
       61 gccgtccacc ttcattggtg cgctgattat ttctatcgtt gccggttacc ttgtgaaatg
      121 gatgaaccag aaaatccagt taccggattt cctgttagcg ttcaaaacca catttttgct
      181 accgattctt tccgccattt ttgtcatgct ggcgatgtac tacgtcatca ccccctttgg
      241 tggctggatc aacggcggta tccgtactgt gctgactgcc gcaggtgaga agggcgcgct
      301 gatgtacgca atggggatcg ccgcggcgac agcaatcgac cttggtggcc cgatcaacaa
      361 agccgcaggt ttcgttgcct tcagctttac cactgaccac gtattaccgg tcaccgctcg
      421 ttctatcgct atcgttattc cgccgattgg tctgggtctg gcgaccatta ttgaccgtcg
      481 tttaaccggc aaacgcctgt tcaacgctca actttatccg cagggtaaga ccgcgatgtt
      541 cctggccttt atggggatta gtgagggtgc gattccgttt gcgctggaaa gccccatcac
      601 cgccattccg tcatatatgg tcggcgcgat tgtcggctca accgcagctg tctggctggg
      661 cgcagtgcaa tggttcccgg aatccgctat ctgggcatgg ccgctggtca ctaacctggg
      721 cgtttatatg gcggggatcg cgctgggggc aatcattact gcgctgatgg tggtgttcct
      781 gcgtctgatg atgttccgca aaggcaaatt gttaatcgat agcctgtaag aaggaaaatc
      841 cagatgacat tactcgcttc gctgcgcgac tggcttaagg tgcaacaact ggatgcggtg
      901 cttctctcct cacggcagaa caaacagccg catctgggga tctccactgg atcaggctat
      961 gtggttatta gccgtgaaag tgcgcacatt ctggtagatt cgcgctattt cgttgaggtg
     1021 gaagcccgtg cgcaaggcta ccagctgcat ttgcttgacg cgacgaacac gcttaccact
     1081 atcgtcaatc aaatcattgc cgatgagcag ttgcaaacgc tcggttttga aggccagcag
     1141 gtgagttggg aaaccgcgca ccgctggaag tctgaactca atgcgaaact ggttagcgcc
     1201 acgccggatg tgctgcggca aatcaaaacg ccagaggagg tggagataat ccgcctcgcc
     1261 tgtgggattg ccgatcgcgg agcggagcat attcgccgct ttattcaggc ggggatgagc
     1321 gagcgcgaga tagccgctga actggagtgg tttatgcgcc agcagggcgc agaaaaagcc
     1381 tcttttgaca ccattgtcgc cagcggctgg cgcggggcgc tgccgcacgg caaagccagc
     1441 gacaagattg ttgcagcggg cgagtttgtc actctcgatt ttggtgcgct gtatcagggc
     1501 tactgctgat atgacgcgca ccttgctggt gaatggcgaa ggggtgagcg ccgaatctca
     1561 cccgctgttt gacgtctatc agattgtcct gcaggcacag ctcgcggcaa tctctgcaat
     1621 tcgccccggc gtgcgctgcc agcagattga cgacgccgcg cgccgggtca ttaccgaggc
     1681 tggatttggc gactatttcg gtcataacac tggtcacgct atcggcattg aagtccatga
     1741 agatccgcgt ttttcaccgc gggacaccac gacgctacag ccaggcatgt tgctgaccgt
     1801 ggagccgggg atttatttgc tagggcaagg gggcgtgcgc atcgaagatg ttgtgctggt
     1861 taccccgcaa ggcgcagaag tgctctacgc catgccgaaa acagtgttgc tcacgggaga
     1921 ggcataatgg atttatcgct attaaaagcg ttgagcgagg cagatgcgat cgcctcctcg
     1981 gaacaggaag tgcggcagat cctgctggaa gaagcggctc gcctgcaaaa agaagtgcga
     2041 tttgatggtc tgggatcggt gctgatccgc ctcaatgaat cgacaggtcc gaaggtgatg
     2101 atctgcgccc atatggacga agtgggattt atggtgcgca gcatctcccg cgaaggcgct
     2161 attgacgtgc tgccggttgg caacgtgcgc atggctgccc gccagctgca gccggtgcgc
     2221 atcaccaccc gtgaagagcg caaaattcca ggcctgcttg acggcgaacg gcaggggaat
     2281 gacgttagcg ccatgcgcgt ggacattggc gcgcgcacct atgacgaagt gatgcaggcg
     2341 ggaattcgtc caggcgatcg cgtcaccttc gataccactt ttcaggtcct ccctcaccag
     2401 cgggtgatgg gcaagccttt gatgatcgcc tcggttgcta tctgctggtg acgttactac
     2461 gtgagctgca cgacgccgaa ctgcctgcgg aagtgtggct ggtggcaagt tccagcgaag
     2521 aggtgggatt acgcggcggg caaactgcta cccgcgcggt gtcgccggac gtcgccattg
     2581 tgcttgatac cgcctgctgg gcgaaaaact ttgattatgg cgcggctaac catcgtcaga
     2641 ttggtaacgg gctgatgctg gtgttaagcg ataaatcgct gattgcgccg ccaaaactta
     2701 ccgcgtggat tgaaaccgtg gcggcagaaa ttggcgttcc gttgcaggcg gatatgttca
     2761 gtaacggcgg cacggacggt ggagcggtgc acttaaccgg tactggcgta cccacagtgg
     2821 taatggggcc tgccacccgc cacggacatt gcgcggcatc gattgccgat tgccgcgaca
     2881 ttttgcagat ggagcaactt ttatctgccc ttattcaacg tcttacgcgt gagacggttg
     2941 ttcaactgac ggatttcaga tgatctcctg attaacccgg agcggttatg ttaacgattc
     3001 aatttctctg tcctttgcct aacggtctac atgctcgtcc ggcgtgggaa cttaaagaac
     3061 agtgcagcca gtggcaaagc gaaatcacct ttatcaacca tcgccagaac gcaaaggccg
     3121 atgctaaaag ctcgctggcg ttgattggaa ctggcacctt atttaatgac agttgcagcc
     3181 tgaacattag cggcagcgat gaagagcagg cgcggcgcgt gctggaagag tacatcctgg
     3241 tgcgctttat cgacagcgac agcgttcagt cgacgcaggc agaactgacg gcgcatccgc
     3301 tgccgcgttc attaagccgc cttaacccgg atttactgta cggcaatgtg ctggcaagcg
     3361 gcgtcggcgt gggtacgctg accctgttac aaagcgacag cctcgacagt tatcgggcaa
     3421 tccccgccag tgcgcaagat tccacccggc tggagcacag cctggcaacg cttgccgagc
     3481 aactgaacca gcaattgcgt gagcgtgacg gcgaaagcaa aactatcctc agcgctcatt
     3541 tgtcgctgat tcaggatgat gaatttgcag gcaatatccg tcgcctgatg acagaacagc
     3601 atcagggact gggggcggcg atcatcagca atatggagca ggtttgcgcc aaactatctg
     3661 cttctgccag cgattatctg cgcgagcggg tcagcgacat tcgcgatatc agcgaacagt
     3721 tgctgcatat cacctggccg gaactgaagc cgcgcaacaa tctggtgctg gaaaaaccga
     3781 ccattctggt ggctgaagat ttaaccccaa gccagttttt gagccttgat ttgaaaaatc
     3841 ttgcgggtat gattctggag aaaaccgggc gcacctcgca tacactgatt ctggcccgtg
     3901 cctcggcgat cccggtactg agtggcgtgc cgctggatgc gattgcccgt tatgccgggc
     3961 aacctgcagt gcttgacgcc cagtgcggcg tgctggcgat taacccgaat gacgcggtga
     4021 gcggttatta tcaggtcgcg cagacgctgg cggataaacg ccaaaaacaa caggcgcagg
     4081 ctgccgcgca gctggcctat tcccgtgata acaagcgtat tgatattgcg gcgaatatcg
     4141 gcaccgctct ggaagcgcca ggcgcgtttg ccaacggcgc ggaaggtgtc gggctgttcc
     4201 gtaccgaaat gctctatatg gatcgcgaca gcgcgccgga cgagcaggag caatttgaa
//
LOCUS       DADLSE010000235         4232 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLSE010000235 DADLSE010000000
VERSION     DADLSE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.237"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            64..1122
                     /gene="yeeE"
                     /locus_tag="JN692_004007"
     CDS             64..1122
                     /gene="yeeE"
                     /locus_tag="JN692_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7266141.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1136..1363
                     /locus_tag="JN692_004008"
     CDS             1136..1363
                     /locus_tag="JN692_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7266142.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN692_004009"
     CDS             complement(1406..2830)
                     /gene="sbcB"
                     /locus_tag="JN692_004009"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7266143.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            3012..4190
                     /locus_tag="JN692_004010"
     CDS             3012..4190
                     /locus_tag="JN692_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7266144.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
BASE COUNT         1037 a          989 c         1090 g         1116 t
ORIGIN      
        1 ttggtctgca ttccaccagt catgaaaaat ttccccatta acattcatct ggttaattaa
       61 tttatgtttt caatgatatt aagcgggcta atttgtggtg ctctgctggg atttgtcatg
      121 cagcgtgggc gtttttgcct gactggtggt tttcgtgata tgtatatcgc gaaaaataat
      181 cgcatgtttt acgccttgct gattgcaatt tcggtacaaa gcgtgggggt ttttgcgtta
      241 attcaggcgg gcctactgac ttacgaagcc ggggcgttcc cgtggcttgg tactgttata
      301 ggtgggtatc ttttcgggct gggaattgtt ctggcgggtg gatgtgccac cgggacctgg
      361 tatcgcgcgg gtgagggatt gatcggcagt tggatcgcgc ttttcactta tatggtgatg
      421 agtgcggtga tgcgttctcc acacgccagt ggtttaaatc aaaccttgca gcactacact
      481 actgaacata actctattgc tgagactttt aatttgtctg tgtggccgtt ggttaccgtt
      541 ttgctggtga taacgctctg ggtggtgatg aaagagttga agaagccaaa actcaaagtc
      601 gcgaccttac cgccgcgccg aaccgggatc gctcatattc tgtttgaaaa acgctggcat
      661 ccctttgtca ctgctgtact catcggtttg attgcgcttt tagcctggcc cctgagtgaa
      721 gcaaccggac gcatgtttgg gttaggagtc acttccccaa cggccaatat tctgcaattt
      781 ctggtcgcgg gtgacgtgaa atacattaac tggggcgttt tcctggtgtt agggatcttc
      841 gtgggggcat ttattgcagc caaagcgagc cgtgagttcc gcgttcgcgc agctgatgca
      901 caaacaacat tacgtagcgg gttaggtggt gtactgatgg gcttcggtgc cagtattgca
      961 ggtggttgct ctatcggtaa tggactggtt atgactgcaa tgatgacctg gcagggctgg
     1021 attggccttg tatttatgat tctcggagtc tggactgcgt cctggcttgt gtatgttcga
     1081 ccgcagcgta aggcgcgact ggcaactgct gcggcaaatt aagtaaggtg taaagatggc
     1141 gattaaaaag ctggatgtgg tgacgcaggt ctgtccgttt ccactcatag aagcaaaagc
     1201 agcactggca gaaatggcca gcggcgatga gttggtaatt gagtttgatt gcacccaggc
     1261 aactgaagca atcccacagt gggcggcaga agaaggacat gccatcaccg attatcagca
     1321 gattggcgat gcagcgtgga gcatcaccgt tcaaaaagcc tgactcaaca ttgtcctccg
     1381 ccgtaccagc ggcggcggct tcaaattaga cgatctcttc cgcgtactgc caaagtgctt
     1441 ttaacagcgc cactttctct ttgtcatcgg catattgttg taccagcatc tgcaattcat
     1501 cagcataacc ctgcaaaaac tctggcgtga agacctggcg gcggtgctcc agccagcgtt
     1561 gctgctcggc ataatccagc gtccccggga agttgcgtgc ccgataattg aacaacaact
     1621 tttcaattcg tttatcaaca aaagtgatat ccagtgccgg taaattacgc ggctcggttt
     1681 ccagcacaat tttcattgct gcacgatcgg cgtcactgaa aaagccatta taaagctgtg
     1741 catccacatt atctgaaggt gtaaacggtt cggcttccgc gaatatcgct accacttttt
     1801 cacgcacttg cggattttca cgcagaattt ttaggttatc gaggcaatgc tgacgattaa
     1861 ttcccagtcg gtcggcatct tccgggcgta gcgtattcgc ctgggccagt accggacatt
     1921 tattgatatg caccagctta accggaacgg cggcgttatc gccaagatcg gcttttgcgg
     1981 tatataaacg ctcgcgcaat gtgtcgatat ccagctccag taatggcgaa atgtctcctg
     2041 ccaaatccgc cataattacg gcattgcgat tttcaggatg ccacgccagc ggtgccaccc
     2101 agctggtatt gccacgccat gccccaaaca ttccggaaac gtgagccagg ggtttcatct
     2161 gcggaacatc aatcaacgcc atcagtttgt gtttattacg atgggtaaag agataatcaa
     2221 acaggcgtgg ctgacgcgtt tttaccagtt tcgccatcgc aatagtggcg tacacatcag
     2281 ccatcgcatc gtgggcgttg ctatgttcaa taccattcgc tttggttaaa tgctcaaggc
     2341 gaaagctcgg tagaccgtca tcattttcag gccagtttat tccttccggg cgcagagcat
     2401 aacaggcacg cataacatcc agtaaatccc agcgcgagtt atcatgctgc cagctccagg
     2461 cgtaaggatc gtagaaatta cgataaaaaa cgttgcgtgt gacttcgtcg tcgaaacgca
     2521 cattgttgta gcccagaata caggtcttcg gtacggtaaa aagcgagtga atacgggcgg
     2581 caaacgcggc ttcgttttct cctttcgccc gtgcctcctg cggggtaata ccggtaatta
     2641 atactgctcc aggccggggt aaatagtcat ccgcgggctt gcagtaaaag acttcgggtt
     2701 cgccgatgac attgaattcg tcatcggtgc gaatggctgc gaactgtgca gggcgatcta
     2761 acgcggggtg cgtgccaaag gtttcgtaat cgtgaaagag gaaggtaggt tgtttatctg
     2821 tgtctgtcat tgtctagcat tgtccactgt agttaattaa ctgtctgtta taactcgaaa
     2881 aacaaccctt ttttcacctg agtatgtcta cgaacgtcaa atatagttgt ttactgtgcg
     2941 ctgttgtcca tgtgatattg tgtaccagat gtgtaccaaa ctattatttc tgagcgtacc
     3001 aaatattatt tatggctata agcgacacaa aactgcgtac tatttatggt aaaccatatt
     3061 cgggcccaca agaagtggct gatgccgatg gcctcagcgt acgaatttca ccgaaggggg
     3121 tcatccagtt ccagtaccgc tatcgctggc atggcaagcc taatcgactt gggcttggtc
     3181 gatacccatc cctgtctttg aaggatgcca gacagatcac tgctgacttg cgaaagctct
     3241 atttctcagg aacggatcca cgcacctatt ttgaagagaa ggtggagaac tccatgacgg
     3301 tcgcccagtg tctcgactac tggttcgaca actacgtctc tacaactctc agagaaaaga
     3361 cccaggcact ttatcgatca acggttatga agcgcatgca tgacgccttc cctaatcgtc
     3421 cggcatcttc tatcacggtt aagcaatggg ttgacctgct taccgaagaa gaaaaagata
     3481 atccacgccg agcaaggcag gtgctaagtc aactaagatc agcaattagt tggtgcatgc
     3541 ggcgtcagtt gatagatagt tgcgcaatta tgagcatcca gccaagggac ttcggctccc
     3601 gcgctgaggt aggggatcgg gtactgtcgt atcacgaact ggctaagatt tggcttgcta
     3661 ttgaaagaag ccgtgcgtct acgtcaaata agctccttca tcagatgctt atgctgtggg
     3721 gggcgaggct ctcagagctt aggctggcaa caaagacaga atttgacctg ctggacaacg
     3781 tatggaccgt accgaaagag catagcaaga tgggtaatgt tatccgccgt ccaatcttcg
     3841 aacaaattaa gccttttctc gaaaaggcca tgacaacgta caatgatgtt cttttccctg
     3901 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
     3961 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
     4021 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
     4081 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
     4141 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
     4201 aagaatccac ccttcaacag cttcacgaag gt
//
LOCUS       DADLSE010000236         4203 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLSE010000236 DADLSE010000000
VERSION     DADLSE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.238"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..486)
                     /locus_tag="JN692_004011"
     CDS             complement(<1..486)
                     /locus_tag="JN692_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7266145.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMELSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PV"
     gene            complement(697..2094)
                     /gene="ccp"
                     /locus_tag="JN692_004012"
     CDS             complement(697..2094)
                     /gene="ccp"
                     /locus_tag="JN692_004012"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7266146.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2499..4148
                     /gene="treF"
                     /locus_tag="JN692_004013"
     CDS             2499..4148
                     /gene="treF"
                     /locus_tag="JN692_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7266147.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGKP"
BASE COUNT         1078 a         1074 c          981 g         1070 t
ORIGIN      
        1 taccggaccg cacactaggt ttggtttatc cgttggtttg cctgcagctt ccatacgctt
       61 gcgccaacgc catttcatcg ccatcccgcc gagcatacag gcctcggaag aaccaatggt
      121 gttggtgcca acggcctgac catttttcgg cgcaggcgca tgccacagat cggcaaccat
      181 atttacgcaa cgcaggtcga tggctgcgga ttgcggatat tcttctttgt cgatccagtt
      241 tttattgatc gacaactcca tcaatttatg gacgttttcg tcgtcccagg tctggcagaa
      301 agtggctagg ttctgacgag cgttgccatc aagatataat tcatcattga taatctggaa
      361 tgcgacatca tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcga tagtagaaat
      421 ggcctttgcg ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg
      481 gtccatttcg aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa
      541 tggaagcaag gcattacagg cttaatttaa ataacaaaat cctaagcaga aaaacgactt
      601 tttataatat aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa
      661 atcgcccaaa cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg
      721 cgtgtacacc ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg
      781 cagctctttg cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac
      841 gtcaccgcga tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg
      901 acgcaattta tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat
      961 ttccccaaag ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat
     1021 aataccacca tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg
     1081 ttgcgccgtc agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt
     1141 aattaatgtt ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg
     1201 cggataaact tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat
     1261 ttcgtcccag gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc
     1321 ctgcaatgtt gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac
     1381 cgtcggcgca ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc
     1441 gacgcccccc gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa
     1501 acggggatcg tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt
     1561 ttgcgggatg ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta
     1621 atattccgcg cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac
     1681 cttacccgcc cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac
     1741 ccattcaatc ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg
     1801 cacggcctcg aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt
     1861 cgcgccagga atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca
     1921 tcctttttcg cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac
     1981 atcggcctgt ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata
     2041 gcaaatcgcg acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct
     2101 tccctcacgg tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc
     2161 tgcgagacaa agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat
     2221 tacatgaata taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca
     2281 tatcatgatt gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acaccaaatc
     2341 cgtattttta taaattccat tgatattagt gcgttatatt ttttgacaag ttaataaacg
     2401 tgatccaccg cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag
     2461 cccgataatt agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa
     2521 accctaatcc agacgaactg atgatcgagg tcgatctctg ctatgaactg gacccgtatg
     2581 aattaaaact ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc
     2641 ccgcctctga tgcgctgacg cctgccgatc gctatctcga actgttcgag catgttcagt
     2701 cggcgaaaat tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc
     2761 tggatatttt aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt
     2821 ttgttgaaaa tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc
     2881 aaaattccct gaaagagcat atcgaccagc tgtggccggt gctaacccgc gaaccacagg
     2941 atcacattcc gtggtcttct ctgctggcgc taccgcagtc atatattgtc ccgggcggcc
     3001 gttttagcga aacctactat tgggattcct atttcaccat gctgggactg gcggaaagtg
     3061 gtcgggaaga tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg
     3121 gtcacatccc caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg
     3181 cgctgatggt ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc
     3241 accttaaaat ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg
     3301 cctatcgcca tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc
     3361 gcgacacgcc acgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc
     3421 gcccgcccaa cgaggtgtac cgcgatttac gcgcgggagc ggcctccggt tgggattact
     3481 cttcccgttg gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc
     3541 ccatcgatct gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc
     3601 tgaaaggcga gaaagagaca gaagcgctgt tccgccagaa ggccagtgcc cgtcgcgatg
     3661 cggtaaaccg ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac
     3721 gcgaacaact ggcgctgttt tccgctgccg ccattgtgcc gctctatgtc ggcatggcga
     3781 accatgaaca ggccgatcgt ctggcaaacg ccgtacgcag ccggttactg acacctggcg
     3841 ggattttggc aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac
     3901 cgttacaatg gatggcaatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg
     3961 aaatcgcgcg cagctggctg aaaacggtga atcagttcta tctggaacag cacaaaatga
     4021 tcgagaaata ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt
     4081 tgcaggatgg gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggca
     4141 aaccataata tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc
     4201 agg
//
LOCUS       DADLSE010000237         4183 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.239, whole genome shotgun sequence.
ACCESSION   DADLSE010000237 DADLSE010000000
VERSION     DADLSE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.239"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN692_004014"
     CDS             complement(<1..209)
                     /locus_tag="JN692_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7266148.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JN692_004015"
     tRNA            complement(324..399)
                     /locus_tag="JN692_004015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JN692_004016"
     tRNA            complement(406..480)
                     /locus_tag="JN692_004016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JN692_004017"
     tRNA            complement(597..681)
                     /locus_tag="JN692_004017"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JN692_004018"
     tRNA            complement(690..765)
                     /locus_tag="JN692_004018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JN692_004019"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JN692_004019"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7266149.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN692_004020"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN692_004020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7266150.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN692_004021"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN692_004021"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7266151.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1039 c          932 g         1133 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tag
//
LOCUS       DADLSE010000238         4107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLSE010000238 DADLSE010000000
VERSION     DADLSE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.240"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(953..1144)
                     /locus_tag="JN692_004022"
     CDS             complement(953..1144)
                     /locus_tag="JN692_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7266152.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1208..1531
                     /locus_tag="JN692_004023"
     CDS             1208..1531
                     /locus_tag="JN692_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAY7266153.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1521..2231
                     /locus_tag="JN692_004024"
                     /pseudo
     CDS             1521..2231
                     /locus_tag="JN692_004024"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2209..2739
                     /locus_tag="JN692_004025"
     CDS             2209..2739
                     /locus_tag="JN692_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7266154.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQTNLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2746..2979
                     /gene="mbeD"
                     /locus_tag="JN692_004026"
     CDS             2746..2979
                     /gene="mbeD"
                     /locus_tag="JN692_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7266155.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            complement(3524..3991)
                     /locus_tag="JN692_004027"
     CDS             complement(3524..3991)
                     /locus_tag="JN692_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266156.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     SFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          992 a          935 c         1216 g          964 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcttg gtcttttgct cacctgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cttggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagtcag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccctgacac ccgctaacac ctgctgacgc gccctgacgg gcttgtctgc
      901 tcccggcatc cgctcacaga caagctgtga ccgtctccgg gagctgcata tgtcagaggt
      961 tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt
     1021 gaagcgattc acagatatca gcctgttcat ccgcgtccag ttcgttgagc ttctccagca
     1081 gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag
     1141 tcactgatgc ctccgtgtaa gggggatttc tgttcaggag gtaatgatac cgatgaaacg
     1201 cgagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg
     1261 tgagggtaaa cagctggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg
     1321 ttccgggaaa ctgccgacac tggcaccgcc gttactgcgt cagctggctg ccatcgggaa
     1381 taacctgaat cagacagcga ggaaggtgaa cagcgggcag tggtcttccg gtaaccgggt
     1441 tcaggtggtg gccgcactga tggccatcgg ggacgagctg cgccggctgc gtctggccgt
     1501 cagggaacag gggacgcggg atgatagtta agtttcatgc ccggggaaaa ggtggtggca
     1561 gtggtccggt tgattacctg ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc
     1621 agggtaatcc ggaagaagta cgggaactca tcgatgccac gccatttgcg aagaaataca
     1681 cgtccggtgt tctgtcattc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga
     1741 tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt
     1801 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
     1861 tacagaccgg aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg
     1921 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
     1981 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
     2041 ttacacgcgg gatagacgcg ctttaccatg ccggagagat aaaaggccgt caggatgtga
     2101 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
     2161 ttgcagaccc gaacggcggg aagaatatca gactgaaagg agcattctat gagcaatctt
     2221 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
     2281 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
     2341 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
     2401 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
     2461 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
     2521 gggacgctct ggtatcttgg cgggatgata cagacgaacc tggaagaaat caggaagcag
     2581 aacgagagtc tggagaagct gaacgcgaag acatggggcg tgcgttatca cgaggggagc
     2641 aacggacgtt ttctggtgtt gcccgagggg atgaaagccg aaacgaactg gacgatggaa
     2701 aacgggaaac agaatgccgt aaggctggta caggagtaac agagcatgac ggagctggaa
     2761 agacatttgc tgaacgcatt agagcaactg caacaggact atatgcagcg gctgaacgaa
     2821 tgggagagcg ccttcgtgga attgcagaaa atgttttcgc ttacgcaacg ggacaacgcg
     2881 atgctgaacg agcgggtcat gcagttgagt cagcaagtgc agcacttgag cgagcggaca
     2941 gaacgcttga gccagttata cagcgagaac aggagataag ggacgaacgg ctgatacagg
     3001 aatgcgaaca tgcgttatcc ctggagcgtg agcgccagct ggaaatacag gaacggacac
     3061 tggatggccc ttcgctggga tggtaaccag agacaggcta aaatgttggc cgcgcgtttc
     3121 cggtgcattt tttgggataa tggttagttg ggggcaccgg gaacgcgctt cagtcagacc
     3181 ttgctgaaaa cacaggaacg cgttaaaacg catacgggcg cgattctggt gtgtcttttg
     3241 ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc cctgaacttc tgttttcccg
     3301 ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa aatccgtttc agctgtctct
     3361 ggctggcaat tcctgcgtga ttcacatggc tgcatagcta tgcatgtggt taaaatttac
     3421 cgggtgcgat cgcggcagtt tttcgggggg gttgttgcca tttttacctg tctgctgccg
     3481 tgatcgcacc gaacgcgttt cagcggtgcg tacaattaag atattacgtt aaattgtgag
     3541 ggaatggtcc ccatatactg actttatcat agggataggg ttggtagtgg gtcgattggg
     3601 tttcggcatt gtttccgtta atgccaattg catttttgta tttgatgcaa cagaaggcgt
     3661 gagattctcc tgggcctgga aaggataagt gtttagaatt gatggcaaaa tatcttccgt
     3721 ccgatttttg aagtagttgt tgtttggctg tctcccaaag ggtgctgtta caaacttctt
     3781 gaatttgtag tttttgcagg gctcttatta ctgcgttgtc atgttccaat tctttagggg
     3841 agtgtattat tctctctttt aataagctgt ttaccacagg gtttagttgt tgtctaaagc
     3901 acgcagataa tgccattgct acacaatttt gttcccgagg aagcatgttt ggcggggtat
     3961 gccgtgtttg atgtgatatg tctattctca tagaaggtct ctggcttata gcatcaacaa
     4021 atacggctat aaaacaaaat tttcatagag tgattcaaga tcttcttgag atcctttttt
     4081 gtggcgcgta atctgctgcc tgcaaac
//
LOCUS       DADLSE010000239         4094 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLSE010000239 DADLSE010000000
VERSION     DADLSE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.241"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..293
                     /locus_tag="JN692_004028"
     CDS             <1..293
                     /locus_tag="JN692_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266157.1"
                     /translation="MGLANKGWIKGEPQGGGWMIKPLGRWSLIMEIDEGFAVGMSPAE
                     LSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE"
     gene            303..545
                     /locus_tag="JN692_004029"
     CDS             303..545
                     /locus_tag="JN692_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HAY7266158.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKTLSGVMKIFHRSGSPPLRRPST"
     gene            581..3948
                     /locus_tag="JN692_004030"
                     /pseudo
     CDS             581..3948
                     /locus_tag="JN692_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1079 a         1039 c         1017 g          959 t
ORIGIN      
        1 tcatggggct ggcaaataaa ggctggataa agggcgaacc acagggtgga ggctggatga
       61 tcaaacctct cggtcgctgg tcgttaatca tggaaatcga cgaaggtttt gcggtgggta
      121 tgtcaccagc cgaacttagc gctgagcagc tcttaagcaa gctgtggcta tgggaaggca
      181 aagcggaaag ctatggctgg gggagtaatt caacacagga agcgcagttc tccgtactcg
      241 atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa taaggaaaga
      301 gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg aaatataaaa
      361 aaggcaaaac accactcagt catcgcaatt ggccgggcga accagtgcct gttatcactg
      421 aaagcatcat tcagacactg ggtgatgaat tgctacaaaa agctgagaag aaaaaaacat
      481 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
      541 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
      601 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctagaata
      661 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
      721 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
      781 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
      841 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
      901 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
      961 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gatgctactg accccaaagc
     1021 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     1081 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     1141 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     1201 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     1261 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     1321 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     1381 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     1441 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     1501 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     1561 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     1621 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     1681 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     1741 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     1801 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     1861 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     1921 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     1981 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     2041 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     2101 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     2161 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     2221 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     2281 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     2341 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     2401 acttgccctg cgtttacttt acgaacatgg gcatggcgaa ttgctacaaa ctgtagccaa
     2461 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     2521 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     2581 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     2641 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     2701 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     2761 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     2821 aagcacggca cgggatctaa cgacacatat cctcgcctgc ccacaagaag gcaaatctgc
     2881 ccgtgcggtc agtggcctga acatccttac cctgatgaat aatgatatgg cgctgatgca
     2941 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     3001 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     3061 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     3121 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     3181 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     3241 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     3301 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     3361 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     3421 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     3481 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     3541 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     3601 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     3661 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     3721 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     3781 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     3841 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     3901 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     3961 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     4021 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     4081 agacttgaga tcca
//
LOCUS       DADLSE010000240         3998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLSE010000240 DADLSE010000000
VERSION     DADLSE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.242"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            292..888
                     /gene="wrbA"
                     /locus_tag="JN692_004031"
     CDS             292..888
                     /gene="wrbA"
                     /locus_tag="JN692_004031"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7266159.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            909..1136
                     /locus_tag="JN692_004032"
     CDS             909..1136
                     /locus_tag="JN692_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266160.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN692_004033"
                     /pseudo
     CDS             complement(1174..2415)
                     /gene="agp"
                     /locus_tag="JN692_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            complement(2708..3964)
                     /locus_tag="JN692_004034"
     CDS             complement(2708..3964)
                     /locus_tag="JN692_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7266161.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT         1111 a          924 c          840 g         1123 t
ORIGIN      
        1 ggactaagct tagtgaactg acgcgggcag acccgcgttt ggtaatatcc tgcaacagct
       61 tcttagcgag aatagatatc atctccaata attatccata agccgctctt atgattaaaa
      121 aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt
      181 tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt atctttaaat
      241 aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga aatggctaaa
      301 gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct
      361 gagggtgcaa gcaaagtgga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg
      421 ccgccgcaat tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg
      481 caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg
      541 tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta
      601 tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaacc
      661 atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac
      721 gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc
      781 accatcgcag gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt
      841 tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga
      901 ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc
      961 gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa
     1021 tggcagaaaa gatttgggaa gaaggtagtg atgaagtgtt agtcaaagcc tttgctaaga
     1081 ccgataaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag
     1141 cgactccccc gcttctcgcg ggggagtttt ctgttatttc accgcctcat tcaacacgct
     1201 atcaaactta tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag
     1261 cgtcacgcgc tgcgcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc
     1321 actctggtac acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg
     1381 ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg
     1441 tttgaaatcc agcgccgtta acagagaggc tatgttggag tcgtgcccaa ctaacaccgt
     1501 gattttcgga gcactggcgc gatcggtgac cagggctttg tcgatataac tgaccagcgg
     1561 tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg ctgtcctagt aaccgttttt
     1621 cagcttcgac aataccttcc actgctggtc agatttgatt tctccctagg ccacctgatc
     1681 catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt tgccgacttt
     1741 cagcggcccg gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc
     1801 caccagcgaa cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt
     1861 ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc
     1921 caccgcctgt tcactgaatg cggcggaatc atcggtgatc atcgggttaa aggttgggtc
     1981 catagtgccc attttttcct ggtgatgcac aggaatatca cacccaggga atgcaccagt
     2041 aataaagaac tgggcggtag cgacggtacg ttgcagacta ttggcatagg cataaacggt
     2101 gtccggcggc gggcattccc ccgatttcac tatcccctgc tctgccagcc attcacgcat
     2161 gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac
     2221 gtcccattcg ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag
     2281 cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg
     2341 tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat
     2401 tagcgttttg ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa
     2461 atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat
     2521 atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg
     2581 cgtacgccgc tgataattgc aactgtggct aaacagaata tctgcggcgt taccaataca
     2641 gagacaatat ttaccaataa aaaagtgtga gactaataac aatggttttt agcatatgcc
     2701 tttgccatta agttgcataa cgatttaaat agtcaagttg cgcattcatt tccttatttt
     2761 ttaacttata agcctgagaa aaaagttcag gagggaggta aacatcataa tgattgccag
     2821 tgtttattaa ataaccatac tcaagatctt catctgttga aaaggattca tttcccaagg
     2881 agattgatgc cacattacta ttaacaataa ataatgagca aatgctatct gaaagaacag
     2941 caagaaaatt cttcaccgct ttaatcctat ccgacgtgtc ggtatctaaa gcaagcaaat
     3001 tcttgttatc attaaatata aactcgggta aatgcagtat gcattttttg tcattcaagc
     3061 aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacataaaa ctacccacac
     3121 cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtctttaca tcttccctga
     3181 gattatagaa tgccgtatca acaagagcct cgatcgtttg tttatcaaca attttaataa
     3241 actcttcgta tacttgcttt tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt
     3301 cttttaaaac cagtatggct cttaacagac aatatccatc gccatctact ggagcgtatt
     3361 ttaatccata tgttttatta atttcactct gaataaaaga tatgccagac aaatcaatac
     3421 cagtatctaa atcagatatt cccggcatat ttatctgttc tggctggata ctcatatttt
     3481 catcatctaa acaatactca gaaaaaacat catcacacgc attaatgttt tcaatatatt
     3541 ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa cctctgcccg
     3601 gcaaggacac tctaacaata tcaacatctt gaacatattg tatagtatat gtaatacctc
     3661 ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata aataatgata
     3721 gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcacat tccagacatg
     3781 gagtatcctc ttcatttact ttacgtctga gttcattgta caaccgcata aaacacacca
     3841 gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtactca ttttttatat
     3901 cgttccaggc ctgtttcaaa taattatttg cagtgcaact aattccatgt atattactac
     3961 ccatatatag cgtctataaa atttaataaa taatgacg
//
LOCUS       DADLSE010000241         3918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLSE010000241 DADLSE010000000
VERSION     DADLSE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.243"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            156..908
                     /locus_tag="JN692_004035"
     CDS             156..908
                     /locus_tag="JN692_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7266162.1"
                     /translation="MPVSLSAQLGSKEQADVRLAGSVMSALRVSMPGIVQSFDPDTVT
                     VVVQPAIKGYEPDSNGINQSTTLPLLVDVPVVFPRGGGCTLTFPVKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            908..1261
                     /locus_tag="JN692_004036"
     CDS             908..1261
                     /locus_tag="JN692_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266163.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVRFST
                     EIDTLYGITTVTSEA"
     gene            1261..2460
                     /locus_tag="JN692_004037"
     CDS             1261..2460
                     /locus_tag="JN692_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266164.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDGNNVIWRLPASVVVGVDGTVMATARCSVSGAVAALA
                     GTITEINTPTRGWVSVINPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVAGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQVISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGWNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            2457..3137
                     /locus_tag="JN692_004038"
     CDS             2457..3137
                     /locus_tag="JN692_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7266165.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     DISDVSCELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGILL"
     gene            3464..3918
                     /locus_tag="JN692_004039"
                     /pseudo
     CDS             3464..3918
                     /locus_tag="JN692_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          991 a          827 c         1063 g         1037 t
ORIGIN      
        1 taaaaaaaga agaatgtaga ttggtgctat ctgaaaataa gaaatgagtt cgctaaatta
       61 acttttcacg gtaattgctt ctatcaccat ggaaagaaaa ctagctacat aaaacccgcc
      121 ttcgagtggg ttttttgctt tctggaggca tatgaatgcc agtttcttta agcgctcaac
      181 ttggtagcaa agaacaggcc gatgtaagac ttgctggctc tgtcatgtcg gcgctgcgtg
      241 tttctatgcc cggcatcgtc cagtcatttg atccggacac ggtaacggta gttgttcaac
      301 cagcgattaa aggctatgag ccagactcaa atggaatcaa tcagtcgacg acattaccct
      361 tgctggtgga tgtgccagtg gtattcccgc gcggcggagg ctgtacgttg acttttccgg
      421 taaaagccgg ggatgagtgt cttgtcgttt ttgccgatcg ttgtattgat ttctggtggc
      481 agagtggcgg gatacaggag ccggttgatg acagaatgca tgatttatcg gatgcgtttt
      541 gtattgtagg tccccagtcg caggcgagga agattagcgg tattaatacc agtgccacac
      601 agttgcgtag tgacgacggc agtacctatt ttgagcttaa tcctgatacc aggaaaatta
      661 aaattgtcgc tccgggtggt cttgatgtgg ttgcccctct ggctgatttt tctgagaaag
      721 taaccattca tggcctgtta acctggatgg gtggcatggt ggggtctgtt gtttctggtg
      781 tggcttcaaa aatcactggt gctgttgagt ttttgggtag cgtgaaggct aacggcaagc
      841 caatcgatga tacgcacact catggtggtg ttcagcgcgg tggaagcaat accgacggag
      901 taaactgatg cgatacagac gtgaagacgc cgatggcgat tacacctttg gcagcggtga
      961 tgacacctgg ctgattaact caccggaggc cgtggcgcag gcggtaaaaa cgcgattcga
     1021 attgtggtat gggcaatggt ttctcgacac caccgagggg actccgtgga tccagtccgt
     1081 actcggtaag cagaagccgg aaacctacaa cctggcgatc cgtaagcgca tcctcgaaac
     1141 gcggggcgtt aaatcaatcc tctctttcaa tactacggtg gataccacga cccgacgtgt
     1201 caggttttcc actgaaatcg acactctcta tggaataacg actgttacat cggaggcgta
     1261 atggctctga accttgattc tctcggttta tctgcaaagg taaccgcgga ggggatcagt
     1321 gcgcctgatt atcagacgat actcagcacc ctgattagct attttcagca gatttatggt
     1381 agtgatgcct acctcgaacc ggacagtaaa gacggtcaga tggtggctct gatggcgctg
     1441 gcgattcatg atgccaataa tatggctata actgtctaca actgtttttc accggcaacc
     1501 ggctatgggg ctgcactgac cagtaacgtg aaaataaatg gtatttcacg taaaggcgcg
     1561 acgaactcta cggttgattt gcttcttaca ggaacagccg gaacaaccat cattaatggc
     1621 agcgtgaaag atggtaataa tgtgatatgg cgtttgcctg cgtcagtggt ggtcggtgtg
     1681 gatggtacag tgatggcgac cgcaagatgt tccgtcagtg gtgcagtggc ggcgctggct
     1741 ggaactatca ctgaaattaa tacgccaacc cgtggctggg tttcggtaat taatcctgct
     1801 gcggctactg ttggctctcc ggcagaaact gatgctgagt tacgtatccg ccagtcgcaa
     1861 agtgttgcgt tgccatcaat aaccccattt gaagcactgg atggtgctgt ttctaatgtt
     1921 gccggtgtaa cccgccacaa actctatgaa aacgatactg gttcggagga cggtaacggg
     1981 ttaccgccac actctgttgc tgtaattgtg gatggcggtg atgtgacgga tattgctcag
     2041 gctatcagag ggaataaagg ccaggggaca gccactcacg gtacaacatc cgttacggtt
     2101 ccggataaat acggcaatcc ccatgtaatc aaattctcgc gttccagtga tgtgcctgtt
     2161 tatgcccgga ttaaattaaa agtttttacg ggttatacct cacagatagg gcagcagatc
     2221 cagcaggtta tttccgacta tatcaatagt ctgacgattg gtgattcggt ccttttaagt
     2281 cgcatttact caccggcgaa tcttggcgtg gtgagtggct ggaatgcacg ctattacgat
     2341 attcaggagc tgacgattgg gaaatccccg ggggctttgt cgtcatcaaa cattgatatc
     2401 agatacaacg aatctgcgtc ctgtaccccg gaaaatatcg ttataacggt ggagtcatga
     2461 gcaaatacac cgaactaatc acgaactacc acgccaccaa acctaaattt cttgcgcatg
     2521 ttgatctgat gacccggcca cttattgatg ttgcggctgc caccagaggg ctgattactg
     2581 catttgatat tgactctgcg gttggtgtgc aacttgacat tctgggattg tggatcggac
     2641 gtagccgtgt tgtcagccag cctatctcag gtgtctattt cagctgggat accgacgggc
     2701 ttggatatga tcagggggta tggcagggac catacgatcc tgattccgga tacatgtacc
     2761 tcagcgatga aacttatcgt gtcattctta aagcgaagat tgcgattaat aactgggatg
     2821 gacggaatga ttcgcttccg gcaattcttg acgctgcaac agcaggatcc gggctgcgaa
     2881 tgcagatagt cgataaccag gacatgacga tatcggtctg ggtctttcct gatactgata
     2941 tttcagatgt atcgtgtgag ttaatagcgg caattaaaca ggggtatctc acagtaaaag
     3001 ccgccggggt atgggcgggt ggcattgaaa caccttcggt ggaaacccca tcggaaggct
     3061 caaaattttt tggttttgat atggataacg aattcatcag tggttttgat gtaggagcat
     3121 ggggaatatt actctgatgg cgaaaaatga ctttaaagcg tttgcaacgg gtaaaaatgc
     3181 caatgttatg tcgcaggagg aatgggaagc gttgcctgcg cttttatccg gatttacagc
     3241 agggaaagca tccagtgcgc aagtcaataa ggttattcgg caggccagct ttattgctgc
     3301 agctctggcc cagtttgtaa gtgacaaaac gcaacgggat gtgcttgata atggtgatct
     3361 gcccggtttt gttgaattgc tgggatcggg gtttgctgtt gaatacctga gccgcaagaa
     3421 tccgtttggc gatatcaaat cggatggcac ggtgaaaacg gctctcgaaa accttggttt
     3481 acaggaaacg gtaaacaagg ctggtaacgc tgtccaaaag acaggcgata ccttgtccgg
     3541 tggacttact tttgaaaacg actcaatcct cgcctggatt cggaatactg actgggcaaa
     3601 gattggtttt aaaaatgatg ccgacagcga tactgattca tacatgtggt ttgaaacagg
     3661 tgacaacggc aatgagtatt tcaaatggag acaccgcctc gctggcggcc agctcaaaga
     3721 gctgatgaat cttaaatggg attcactaaa tattctggtt aatgccgtca ttaatggttg
     3781 ccttggcatt ggtacgacga atgcgttagg tggaaattca attgctttcg gggataacga
     3841 taccggcctg aaacagaacg gcgatggtct gctggatgtt tatgcgaatg gacagcatgt
     3901 atttcgtttt cagaatgg
//
LOCUS       DADLSE010000242         3876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLSE010000242 DADLSE010000000
VERSION     DADLSE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.244"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..284)
                     /locus_tag="JN692_004040"
     CDS             complement(<1..284)
                     /locus_tag="JN692_004040"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7266166.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHII"
     gene            393..1319
                     /locus_tag="JN692_004041"
     CDS             393..1319
                     /locus_tag="JN692_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7266167.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1316..2086
                     /locus_tag="JN692_004042"
     CDS             1316..2086
                     /locus_tag="JN692_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7266168.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            2191..2631
                     /locus_tag="JN692_004043"
     CDS             2191..2631
                     /locus_tag="JN692_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7266169.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            2695..>3876
                     /locus_tag="JN692_004044"
     CDS             2695..>3876
                     /locus_tag="JN692_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7266170.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWSQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TD"
BASE COUNT          950 a          882 c         1012 g         1032 t
ORIGIN      
        1 ataataatgt gttcaacttc gagtacatcc actgcatact gaaccacgga aaggcagttc
       61 aggtcggtgt gaatgaccag gttagcaaca ttacggtgaa caaagagttc gcccggctca
      121 aggccggtta aacgttctgc aggaacgcga ctgtcggaac atccaatcca tagaaagcgc
      181 ggtttttgcg cttgtgccag tttctcaaaa aacccgggat cctcttccac cagcattttt
      241 gaccatagtg cattgttgct gatgagtgta tctatgtctt tcatggaggt taacgacctg
      301 taaccaaata attacgtttg gctaatatag ggcaactccg ggacgattta aaccacagat
      361 aaagtgtaag aacgtaaggt aagtaaaaat ttatgaccat tgcactggaa cttcaacagc
      421 ttaaaaaaaa ctatccaggc ggcgttcagg cgcttcgtgg aatagatttg caggtcgaag
      481 cgggtgattt ttatgcgctt ctcgggccga acggggccgg gaaatcgacc actatcggta
      541 ttatcagctc tctggtaaat aaaacctccg ggcgggtcag cgtatttggt tacgatctcg
      601 agaaagatgt cgtgaacgct aaacgtcagt tgggactggt gccgcaggaa tttaacttca
      661 acccgtttga aaccgtgcag caaattgtgg tgaatcaggc agggtactac ggtgtggagc
      721 gcaaagaggc gtacatccgc agcgaaaaat atcttaagca gctcgattta tggggaaaac
      781 gcaacgaacg tgcgcgtatg ttatctggcg ggatgaagcg ccgtttaatg attgcccgtg
      841 cgttaatgca tgaacctaaa ctactgattc tcgacgaacc gaccgcaggc gtggatattg
      901 aacttcgccg ctcaatgtgg ggctttttga aggatttaaa cgacaaaggc accaccatca
      961 ttctcaccac acactacctg gaagaagcag aaatgctgtg ccgcaatatc ggcattattc
     1021 aacatggtga gctggtggaa aatacctcga tgaaggcgct gctggcgaag ctgaaatcgg
     1081 aaacctttat tctcgatctc gcaccgaaaa gtccgttacc gaagctcgat ggctatcagt
     1141 atcgactggt cgataccgcg acgctggaag ttgaagtgct acgtgagcag gggatcaaca
     1201 gcgtatttac gcagttaagt gagcagggca ttcaggtatt aagtatgcgt aacaaagcta
     1261 accgtctgga agagctgttt gtttcactgg ttaatgaaaa acaaggagat cgcgcatgat
     1321 gcatctttac tgggtggcgc taaaaagcat ctgggcgaaa gagatccatc gctttatgcg
     1381 tatctgggtg cagacgctgg tgccgccagt catcaccatg accctttact ttattatctt
     1441 cggtaaccta ataggttcgc gtattggcga tatgcatggc ttcagctata tgcagttcat
     1501 cgtaccgggg ctgatcatga tgtcggtgat caccaacgcc tacgctaacg tcgcgtcatc
     1561 attttttggt gccaagttcc agcgtaatat tgaagagctg ctggtagcgc cggttccgac
     1621 tcacgtcatt attgccggat atgtcggcgg tggcgtggcg cgtggtctgt ttgttggcat
     1681 tctggtgacg gcaatttcac tgttttttgt gccgtttcag gtgcattcgt gggtattcgt
     1741 tgccttaacc ctggtgctca cggcggtgct attctccctt gcgggtttgc tgaacggcgt
     1801 gtttgccaaa acgttcgatg acatcagcct ggtgccaacc tttgtgttaa cgccactcac
     1861 gtatttgggc ggggtctttt actcactgac tttgttgccg ccgttctggc aagggctgtc
     1921 gcacctgaac ccaatcgttt atatgatcag tggtttccgc tacggcttcc tcggtataaa
     1981 tgatgtgccg ctggtcacta cctttggcgt actggtggtc tttattgtgg cgttttattt
     2041 gatctgttgg tcgctgatcc aacgtggacg tggtttgcgt agctaaggct atttcctctc
     2101 ctctggattc gggggagagg agttttgacg gctatcaccc tttatcaaca atggtcaggg
     2161 tagactgatt ttcggctaag gaggaaggcg atgttaggtt gggtaattac ctgtcacgat
     2221 gaccgggcgc aagagatact ggatgcgctg gagaaaaaac atggggcact tcttcagtgc
     2281 cgggccgtga atttctggcg cggattaagc tctaatatgc tcagccgcat gatgtgcgat
     2341 gctctgcatg aagcggactc tggtgagggt gtcatcttct taaccgatat agccggagcg
     2401 ccaccgtatc gcgtggcttc attattaagc cacaaacact cccgttgcga agtgatttct
     2461 ggtgtcacgt taccgttaat tgaacagatg atggcttgcc gtgaaaccat gaccagttca
     2521 gagtttcgtg agcgtattgt cgaactgggt gggccggagg tgagtagtct ttggcaccaa
     2581 caacaaaaaa atccgccttt cgtcctcaaa cataatttgt atgagtatta acccgcgatt
     2641 ctgatggcgc ttttgctaca ataaaagcgt tgtttcaccc tcggttattt tttcatgtat
     2701 aaacaagctg ttattctcct gctgatgctg tttaccgcaa gtgtcagtgc cgcgttacct
     2761 gcccgttata tgcaaaccat cgaaaatgct gcggtctggt cgcaaattgg tgacaagatg
     2821 gtgaccgtgg ggaatattcg ggccggacaa atcattgccg tggagcccac tgccgcaagt
     2881 tattacgcat ttaattttgg ctttggtaaa gggtttatcg ataaagggca tctcgagcca
     2941 gttcaggggc gacaaaaagt tgaagacggt ttgggtgacc tcaacaagcc gctgagtaat
     3001 cagaacttaa ttacctggaa agatacgccg gtctacaacg cgccgagtgt gggcagtgcg
     3061 ccatttgggg tactggcgga caatttgcgc tacccgattt tgcataaact gaaagacagg
     3121 ttaaatcaaa cctggtatca gatccgtatt ggcgatcgac tggcctatat cagcgcgctg
     3181 gatgcccaac ccgataatgg cctgccggtg ctaacttatc accatattct gcgcgacgaa
     3241 gaaaacaccc gttttcgcca tacttcgacg accacctcgg tacgcgcttt caataaccaa
     3301 atggcctggc tgcgcgacag gggatacgcg acactgagca tggcgcagct ggaaggttac
     3361 gtgaagaata cgatcaatct ccctgcgcga gcggtggtga ttacctttga tgatggcctc
     3421 aagtcggtga gccgctatgc gtatcctgtg ttgaaacaat atggcatgaa ggcgacggtg
     3481 tttattgtta cctcacgcat caaacgtcac ccgcagaagt ggaacccaaa atcgctgcaa
     3541 tttatgagcg tttctgagct taacgaaatt cgcgatgttt ttgatttcca gtcacatacc
     3601 cattttttgc atcgggtaga tggttatcgc cgacccatat tactgagccg tagtgagcac
     3661 aatattctgt ttgattttgc acgttcacgc cgcgctctgg cgcaatttaa tccacatgtc
     3721 tggtatcttt cgtatccgtt tggcggcttt aatgacaaag ccgtgaaggc agcaaacgat
     3781 gccggatttc acctggcggt gacaaccatg aaaggcaaag taaaaccggg ggataatccg
     3841 ttgttactaa aacgacttta tatcttaaga acggat
//
LOCUS       DADLSE010000243         3865 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.245, whole genome shotgun sequence.
ACCESSION   DADLSE010000243 DADLSE010000000
VERSION     DADLSE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.245"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..512
                     /locus_tag="JN692_004045"
     CDS             <1..512
                     /locus_tag="JN692_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheinyl transferase"
                     /protein_id="HAY7266171.1"
                     /translation="SISYAGNMVGVALTTEGECGLDMELQRATRGFHSPHAPDNHTFS
                     SNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQLLPIAGRLKCAHVNH
                     VEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWKSLPDIHSRANNPTSRMMRFAQL
                     STVKSFSPN"
     gene            534..1100
                     /gene="yieF"
                     /locus_tag="JN692_004046"
     CDS             534..1100
                     /gene="yieF"
                     /locus_tag="JN692_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7266172.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMDVIGGACCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1154..2491)
                     /locus_tag="JN692_004047"
     CDS             complement(1154..2491)
                     /locus_tag="JN692_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7266173.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            2656..3321
                     /gene="yieH"
                     /locus_tag="JN692_004048"
     CDS             2656..3321
                     /gene="yieH"
                     /locus_tag="JN692_004048"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7266174.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            3388..3861
                     /locus_tag="JN692_004049"
     CDS             3388..3861
                     /locus_tag="JN692_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7266175.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1029 a          966 c          973 g          897 t
ORIGIN      
        1 tttctatttc ctatgccggg aatatggttg gcgtggcgtt aacaaccgaa ggtgaatgtg
       61 gcctcgatat ggaactacag cgtgcgacgc gcgggtttca tagcccacac gcgcccgata
      121 accacacctt ttccagcaat gaatcgctat ggatcagtaa acaaaacgat cctaacgaag
      181 cgcgggcgca gctcatcacg ctgcgccgaa gcgtgctaaa actaaccggt gatgttttga
      241 atgacgatcc gcgcgatctg cagctgctgc ccattgccgg acgcctgaaa tgtgctcatg
      301 taaatcatgt agaagcgtta tgcgacgcgg aggacgtgct ggtgtggtcc gtggcggtca
      361 cgcccgcgat tgaaaagctc agtgtctggg agttagatgg caaacacagc tggaaaagcc
      421 tgccggatat tcacagccgc gccaacaatc ctaccagccg gatgatgcgt tttgcccagc
      481 tctctaccgt gaagtctttt tcgccaaatt gatagacaac cacaggagtc attatgtctg
      541 aaaaattgca ggtggttacg ttactgggga gcctgcgcaa aggctcattt aatggcatgg
      601 ttgcacgtac cctgccgaaa attgctccgg cgagcatgga agtcaatgcg ttaccttcca
      661 ttgccgacat tcccctgtat gacgctgacg tacagcagga agaaggtttt ccagcaacgg
      721 ttgaagctct ggcggaacag atccgtcagg ctgacggtgt ggtgatcgtc acgccggaat
      781 ataactactc ggtaccgggt gggctgaaaa atgccatcga ctggctttcc cgcctgccgg
      841 atcaaccgct ggccggtaaa ccggtgttga ttcagaccag ctcaatggac gtgattggcg
      901 gcgcgtgctg tcagtatcac ctgcgccaga ttctggtttt cctcgatgca atggtgatga
      961 acaagccgga atttatgggc ggcgtgattc agaacaaagt tgatccgcaa accggagaag
     1021 tgattgatca gggtacgctg gaccacctga ccgggcaatt gaccgcattt ggtgagttta
     1081 ttcagcgagt taagatctaa ataaaaaacc cgccagtaat catgcatggc gggtttttaa
     1141 tgcgctatcg attttaatga gcgtcgataa atacaatctt caggataaac agcagcgcaa
     1201 cgatgattac gcacgggcta aggtcacgca gacgcccggt accaatcttc atcacgcagt
     1261 aggagataaa gcccagcgca ataccttcgg taatcgaaaa gctgaacggc atcatcacgg
     1321 cggtaataaa cgccggaaca gattcagtaa gatcctgcca gttcacgcga gcaagacttg
     1381 aggtcatcaa cacgccaacg tagatcaacg cgccagctgc agcgtagcct ggcaccattc
     1441 ccgccagcgg cgacagaaag ataaccagca ggaacagcag accaacaacc actgccgtca
     1501 gaccggtacg accgccaacc gatacgccgg aagaggactc aatataagcc gtaacggaag
     1561 aagtaccgat aaacgaaccg gtcacggaag agatactgtc gacatacagc gcctgcttca
     1621 tgcgcgggaa tttccccttc tcatccgcca gacctgcttt atcggtcacg ccaatcagcg
     1681 taccggagga gtcaaacaag ttgaccaaca tgaaagagaa aatcacccct gccagcccga
     1741 ggttaaacga cccggctaaa tctacatgac ccacaactgt cattacgctc ggcggtgcag
     1801 aaacgatgcc attgtagtgc acatcaccca gcatccagcc cagcagcgtc gtcaccacga
     1861 tagaaaccag caccgctgcg tgaatgttgc gcgaggccag aatagcaatg atgaaaaagc
     1921 cgaggatacc cagaagtacg ctgtgagaag tcagattacc gatgctcacc agcgtttccg
     1981 agttagcgac aatcacacct gcgtttttca gccccatcat gccgataaac aggccgatac
     2041 cgctggtaat acccacacgc agacttaccg gaatgttagc aatcatccag taacgaacgc
     2101 ggaaaatggt caaaagaagc agtccgacag cgccccagaa gattgcgccc ataccgatct
     2161 gccacggcag ccccattgcc tggacaacca caaaagcgaa aaaggcgtta agccccatcg
     2221 caggagccag cgccaccgga aggtttgcaa acaatcccat cataatgcta ccgaaagcag
     2281 caatcagaca ggttgttacg aagacggcac tggtgtccat gccagcaacg cccagaattt
     2341 gtgggttaac aaatacgatg tagaccatcg tcaggaaggt ggtgaacccg gcaatcactt
     2401 cagttcgtgc cgtagtacca tgttcgcgca gtttaaaaac gcgttccagc atcctctgac
     2461 cagaagtctg ggtggtatgt tgatgactca ttatctattt ccgaacaagg agggaaaatc
     2521 cgtcgctatc gtataccaaa atgcgacaat aggtgcgttt gtgagagact tttttattgg
     2581 atttacttat acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga
     2641 ttaaaaagga aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg
     2701 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
     2761 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
     2821 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
     2881 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
     2941 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
     3001 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
     3061 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
     3121 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
     3181 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
     3241 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
     3301 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
     3361 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
     3421 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
     3481 ttcgcaaaag aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta
     3541 ttgtcgatta ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
     3601 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
     3661 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
     3721 ttgtttgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
     3781 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
     3841 gaaacagact ctttaagtta agcgg
//
LOCUS       DADLSE010000244         3856 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLSE010000244 DADLSE010000000
VERSION     DADLSE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.246"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            344..541
                     /locus_tag="JN692_004050"
     CDS             344..541
                     /locus_tag="JN692_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7266176.1"
                     /translation="MNAHDIEPTILRATGNTHPLDVTFIHEEDLATPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(623..2332)
                     /locus_tag="JN692_004051"
     CDS             complement(623..2332)
                     /locus_tag="JN692_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7266177.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2765..3484)
                     /locus_tag="JN692_004052"
     CDS             complement(2765..3484)
                     /locus_tag="JN692_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7266178.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3609..>3856)
                     /locus_tag="JN692_004053"
     CDS             complement(3609..>3856)
                     /locus_tag="JN692_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266179.1"
                     /translation="WQRWLSLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAE
                     ALNSKIRLLRIKAKGYRNRERFKLGVMFHYGKLNMAF"
BASE COUNT         1120 a          800 c          699 g         1237 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacggag ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcgctat aagatagcga gctcgtcaac gatacagatg
     2461 atctgattat attgtgcaaa aggagaacag gaaaataggc actggaaaag atgaaggcct
     2521 gatgggagca ttaactaatg caaaatccga caggttaata acccgaaaag caatcaaacg
     2581 actcaaacag gagatgcacg ctgaagtggt ggacaactgt tcggatttca gatgactagc
     2641 tcagcagttt atgagccgaa tgctctgtca cgggcaggaa tcaggctgca tacgctgtgc
     2701 caaatctaat cgtagttatt ctaaccagtt ttcaagctac aaggtggtgt agctggcatc
     2761 ttctctactc tatcatcaaa cgataaaatg gttcctctcg taacatttgc tcttgccttt
     2821 cacttccatc tcgatcgctt cgtagttcat tacgatagct gacatatttc cagtcttcag
     2881 gtctaacatc tgacgccgga tactccccgg gagcgatttt gcttcttaat agattagact
     2941 cgagacacat tataaattgt cgtattttat gaagtaataa agcactatat tgcgagcatt
     3001 cctgatctga ttttacatat agcgtaaact gagctcctat ccccacacga gattgctgag
     3061 aaactcgatt catatcagtt atcttccatt tatctatagg actgtcctct gaaaataata
     3121 aaccagacaa aatttggaat gccagaggaa cttgctccct agctatacta atatgaaact
     3181 tgtccccaac aaaatccccc ttactctgag gattttcacg acatgcatga ataaacacat
     3241 ctccactatg gttcacaatc tggaatcctt ggtacatgac agaaaaaaat tgacatatat
     3301 cagagtaagt tggcacgttc gatgctactt tgaaatagta cgctgggtat tgccgtattt
     3361 ggttgtacaa tatattgaag ttatttttca atctttcatt cgcagaaagc atctgcggta
     3421 tttcagacct cacaacattc aaagaatcta aatttagttt tagacagggc ttttttatgg
     3481 gcatagaaaa cgtcctctat aaaatagata tattatctta tattgattgt ttatcatctt
     3541 ttataaaacg atacattata tgtaatgtat gtaagcgtaa gggtcttctc cgatcatggt
     3601 gggaaggctt agaacgccat attcagtttc ccatagtgga acatcactcc cagcttaaag
     3661 cgctcccggt tgcggtagcc cttggctttt attctcagca gcctgatctt gctgttcagt
     3721 gcttccgcat ttccgtttga tacgccatgg cgcatggcgt tcaggatacc gtacaacctt
     3781 ttccctatcg ttttagccac attcttcatc atggggacgt cactgttggc cgccagcgac
     3841 agccatctct gccagt
//
LOCUS       DADLSE010000245         3839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLSE010000245 DADLSE010000000
VERSION     DADLSE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.247"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN692_004054"
     CDS             complement(<1..458)
                     /gene="intS"
                     /locus_tag="JN692_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7266180.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(620..694)
                     /locus_tag="JN692_004055"
     tRNA            complement(620..694)
                     /locus_tag="JN692_004055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(659..661),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="JN692_004056"
     CDS             complement(770..1702)
                     /locus_tag="JN692_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7266181.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTPHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="JN692_004057"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="JN692_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7266182.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3763)
                     /locus_tag="JN692_004058"
     CDS             complement(2933..3763)
                     /locus_tag="JN692_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266183.1"
                     /translation="MDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEK
                     THDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGKSEPTDLFTWYGKD
                     KKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFE
                     SSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVK
                     EIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQAKYQNEINQLSALGVNFDDI
                     "
BASE COUNT          960 a          876 c          855 g         1148 t
ORIGIN      
        1 gcaccgcgat cttcaaatct gcggattact tccagcagtt gcatcggctc aatatcctga
       61 atctcaaggc cgccgatgat gggtaaaatg tcgtcatcaa acatttttgc aagttcagtc
      121 gcatacccta ctgaccagac ttgcttcttg tgttcgtacc attccttgta aattgcacta
      181 aatgaattgt tgttagacga agcctttttc gcctttaccg gatcgatgcc agccgagatg
      241 tctttcctcg cagtccatgc tttatccctt gcctcctgca aagtcataag cggatatttt
      301 ccgacggtca ggattttctc cttaccgtca atcttgtagc gaagctgcca tacctttttc
      361 ccggatacag ggacataaag gtacaggcca ttaccatcga gaaggcggta tggtttttct
      421 ttcggctttg ctgcttcaat ctgcttaacg gtgagcatgg gtaaaaatcc ggtgggtaaa
      481 attattttat ccacttttta cccgtcatgg agtgcggctg tcaacgatct gacgcgaacc
      541 atgacgaact gtgaatatac ggaaggcttg atattcaggg gattttgcgg actggtacgg
      601 atgggagcga actgataaat ggtgtcccct gcaggaatcg aacctgcaat tagcccttag
      661 gaggggctcg ttatatccat ttaactaaga ggacaatgcg gcatgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggggg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcgaca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgcccggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gttcgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcgaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgcctggc
     3001 gattttgaag tcttttttca actttatctt cgagatcctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtccgttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt aaatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctca
//
LOCUS       DADLSE010000246         3816 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLSE010000246 DADLSE010000000
VERSION     DADLSE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.248"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..322
                     /locus_tag="JN692_004059"
     CDS             <1..322
                     /locus_tag="JN692_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266184.1"
                     /translation="TLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN692_004060"
     CDS             complement(386..901)
                     /gene="luxS"
                     /locus_tag="JN692_004060"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7266185.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN692_004061"
     CDS             complement(1051..2607)
                     /gene="gshA"
                     /locus_tag="JN692_004061"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7266186.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(2680..3108)
                     /locus_tag="JN692_004062"
     CDS             complement(2680..3108)
                     /locus_tag="JN692_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7266187.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN692_004063"
     CDS             complement(3105..3671)
                     /gene="yqaB"
                     /locus_tag="JN692_004063"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7266188.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          958 a         1022 c          899 g          937 t
ORIGIN      
        1 aacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc
       61 gatgcaccat gcgcagttga ctgagtcggt aaacccgttc aacccgaatg cccaggcgat
      121 gtacagtcaa ctggaagggc ttgggatgac gcaacagcag gcatcaggct ggattgccca
      181 gcagatcacc aatcaggggc tgattatttc cgccaatgag atcttctgga tgtcagccgg
      241 gatattcctc gtactgctgg ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg
      301 cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca
      361 attaattgtg aagatagttt actgactaga tgtgcagttc ctgcaacttc tctttcggca
      421 gtgccagttc ttcgttgctg ttgatgcgta cgtcacgttc cagaatgcta cgcgcaatat
      481 cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg
      541 ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag
      601 caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca
      661 ttggcgagat atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag
      721 caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc
      781 agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca
      841 cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca
      901 tttagccacc tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc
      961 tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg
     1021 gccactcacg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa
     1081 cggttcggta tcagcggttt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc
     1141 tacaaaatcc tcttcccgca gaatttccag cggctcttca cgcagcagat tacggtaggc
     1201 ttctgcaaac gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg
     1261 ggcagagaaa gtcagatcgg gattatcgaa gcaggcaacc agttcatcac acactttctg
     1321 atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa
     1381 cagatcttta cccacctgca gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt
     1441 caggcccggt ttgcgacctt caaggatcac ccggttccag ttaacgcgcg tacaggcaag
     1501 ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag
     1561 gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg
     1621 cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg
     1681 ggtaacgcgt tttggacgaa tcggcgcata cagttcgttt tcaatctgca gcacgttgct
     1741 attgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg
     1801 cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc
     1861 aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata
     1921 cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg
     1981 caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaatga cccaaccgaa
     2041 acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc
     2101 gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg
     2161 cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag
     2221 cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc
     2281 tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt
     2341 ataacgatgc agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt
     2401 aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga
     2461 acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt
     2521 ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc
     2581 cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt
     2641 agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc
     2701 ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa
     2761 gattactggc ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac
     2821 cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc
     2881 tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt
     2941 taaccctcca aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat
     3001 cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct
     3061 gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca
     3121 tccacagcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag
     3181 accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc
     3241 gcgggtttat ggtgtttgac gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat
     3301 cccaggtgcg ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc tacagccatt
     3361 gggcgacgac catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc
     3421 agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg
     3481 gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca
     3541 atcatcgcct gaatatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta
     3601 cggtgcgtag gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa
     3661 cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt
     3721 gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta
     3781 gaaatgagat atttttgaag gaaatttttt ggagaa
//
LOCUS       DADLSE010000247         3794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLSE010000247 DADLSE010000000
VERSION     DADLSE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.249"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN692_004064"
     CDS             complement(<1..479)
                     /gene="fimB"
                     /locus_tag="JN692_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
                     /protein_id="HAY7266189.1"
                     /translation="MRNKADNKKRNFLTHSEIESLLKAANTGPHATRNYCLILLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     "
     gene            1933..2649
                     /gene="nanC"
                     /locus_tag="JN692_004065"
     CDS             1933..2649
                     /gene="nanC"
                     /locus_tag="JN692_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7266190.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2669..3775
                     /locus_tag="JN692_004066"
     CDS             2669..3775
                     /locus_tag="JN692_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7266191.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSYAPMGMAGHVTFVHNGKAYVTGGVNQNIFNAYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
BASE COUNT         1188 a          750 c          778 g         1078 t
ORIGIN      
        1 agtcgcgtat ctattcccat attcgccaaa gcaaaaccac acgaatggcg taacatgtgc
       61 ggatgaatct ccagtgacaa cccggcatta ccaccagaag tcgagataat atggtaaaac
      121 tgttgccgag aaagcggatt ccccttacgt gataaaaata cccactcgct ctcagcatgc
      181 ggataagaag tacggatact caaccagttt tttaaagcct gaacttcttt gttcaatagc
      241 gggtgcgttg ttgaaaagcc tttttttaat cgatggatat atatacactt tgctttaaga
      301 tcaatatccg aaatcctcaa tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa
      361 agcaaaatca gacaataatt acgtgttgca tgaggcccgg tatttgctgc tttaaggagt
      421 gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt attcctcatc
      481 gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata atccaacatt
      541 tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt aagtggacca
      601 gttcttctga gcgaacttaa atggagtagc gatagttact tataataact ttgccataca
      661 aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat acgaatctaa
      721 taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat atgttctgta
      781 acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac tagccgtctc
      841 aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat ggaacgcagt
      901 aatttcttag tggctattat catgctaatt cttagatccc tagcgattat tcctgcctgg
      961 ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat atgggggaac
     1021 gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca ctacgactta
     1081 agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa gtgaaacatc
     1141 atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt gaccagttac
     1201 aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg atggcgggta
     1261 aacccttatt ttcaccactc tcactgagtt agcttgcaaa gctttctttg tttagattct
     1321 aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg aagaccaccg
     1381 caagtgttcg tctggcttca catggcatct tcttcttaga aaaagatcga catattttgt
     1441 gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat ctggtataac
     1501 aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc tatggcaaac
     1561 atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa aggaatatga
     1621 aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg agtaagtgat
     1681 ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact caggtattta
     1741 atctggatct ctgtttattt aaataatgtg aaaagagaat tttcacagga gaccttatac
     1801 aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc aaaaaaatat
     1861 tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt atcctacaaa
     1921 tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact gtgcttttcg
     1981 tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg tagtggaagc
     2041 cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg gtgggcaagc
     2101 atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc cgcactcaat
     2161 gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac ggtgcgcccg
     2221 ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt aaaactgtcc
     2281 tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg gaaagcttac
     2341 cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg ggatggatat
     2401 gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct atacagcaaa
     2461 cagaatgatt atcgctatgc aaaccacaag aaatgggcaa cggaaaatgc ctttgttcta
     2521 caataccata tgacgcccga tattacgcca tacatagaat atgactacct tgaccgccag
     2581 ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg tgtgtcattt
     2641 aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc ttgctatcat
     2701 gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc catttaaaag
     2761 tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg caggtacagc
     2821 atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag ctgcattccc
     2881 tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt atgtgtttgg
     2941 cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac acaaatacaa
     3001 ccccaaaacc aatagctggg ttaaattgat gtcgtacgcg ccgatgggca tggcgggtca
     3061 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaatatctt
     3121 caatgcctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta tagataaaat
     3181 caacgcccac tattttgaca aaaaagcaga agattatttc ttcaataagt ttctgttgtc
     3241 ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt acggaacggc
     3301 tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg aagccaaacc
     3361 aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt taaaatggaa
     3421 taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg ggataagcaa
     3481 tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa attaccagaa
     3541 cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata ttcatctttg
     3601 gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct acggagtatc
     3661 attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg gcaaagcggt
     3721 gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa actaacgctt
     3781 cagggccccg gtaa
//
LOCUS       DADLSE010000248         3780 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLSE010000248 DADLSE010000000
VERSION     DADLSE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.250"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1064
                     /gene="aldA"
                     /locus_tag="JN692_004067"
     CDS             <1..1064
                     /gene="aldA"
                     /locus_tag="JN692_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7266192.1"
                     /translation="IQSDRPGENILLFKRALGVTTGILPWNFPFFLIARKMAPALLTG
                     NTIVIKPSEFTPNNTIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMT
                     GSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCN
                     CAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARA
                     VEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAIS
                     MANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGA
                     DGKHGLHEYLQTQVVYLQS"
     gene            complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN692_004068"
                     /pseudo
     CDS             complement(1106..2107)
                     /gene="gap"
                     /locus_tag="JN692_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2296..2826
                     /gene="cybB"
                     /locus_tag="JN692_004069"
     CDS             2296..2826
                     /gene="cybB"
                     /locus_tag="JN692_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7266193.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3071..3244
                     /locus_tag="JN692_004070"
     CDS             3071..3244
                     /locus_tag="JN692_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266194.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN692_004071"
     CDS             complement(3316..3465)
                     /gene="hokB"
                     /locus_tag="JN692_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7266195.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(3565..>3780)
                     /locus_tag="JN692_004072"
     CDS             complement(3565..>3780)
                     /locus_tag="JN692_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7266196.1"
                     /translation="RKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEY
                     YNSRRISLKLKGLTPNEYRNQTYMPRV"
BASE COUNT          978 a          853 c          928 g         1021 t
ORIGIN      
        1 ttattcaaag cgatcgtcca ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
       61 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
      121 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatacga
      181 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
      241 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
      301 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
      361 ccaaagtgtg tctggaatta ggtggtaaag caccggctat cgtaatggac gatgccgatc
      421 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
      481 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatcggctgg
      541 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
      601 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
      661 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
      721 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
      781 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
      841 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
      901 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
      961 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     1021 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     1081 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     1141 gctgcgtgac gaagccatat tcgttatcgt atcaggcgac cgttttcacc agttgtaaat
     1201 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     1261 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     1321 cttgtttaag tgcgttattc acctcttcgg cagtcacttt ttttccgaga atggacacca
     1381 gctcagtgac cgaacctgtt ttcaccggca cgcgttgcgc atgacctttc agtttgccgc
     1441 tcagttccgg gatcaccaga ccaatggctt ttgccgcccc cgtagtgtgg ggaatgatat
     1501 tttctgccgc tgcgcgtgaa gcacgtaaat ctttaccacg tgggccatcc accagtgact
     1561 gggtgccagt ataggcatga atggtcgtca tcgtgccgac ttctatcccg aaactgtcat
     1621 gcaaggcttt ggccatcggc gcaaggcagt tagtggtgca tgacgccacg gaaacaatgg
     1681 tgtcgttgcc atccagagtg tcgtcattaa cgttataaac gatggttttc atttcaccgg
     1741 caggagcgga aatcaacacc ttcttcgcac cagcatcaag atgcgcctgc gatttatcgg
     1801 cggaggtata aaagccagta cattcgacaa tgatttctgc ccctttcgct ttccacggaa
     1861 tatttttagc ctctttttcg gcgtaaaccg cgatactttt cccatcaacg ataagtgaat
     1921 cttccgtaaa atcaacgctc caggggaacg gaccatagtt tgaatcatgt ttcagcagat
     1981 aggcgagaat ttttggggaa gtaagatcat taatagcgac aacgtctatg ttgcttttga
     2041 cttcaagtaa acgacgcaac accagtcgac cgatacgacc aaaaccgtta ataccaactt
     2101 tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt ccaggcttag
     2161 accaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt actgaaaacg
     2221 ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga aaattatgtt
     2281 aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat tcactggctg
     2341 gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcgtggttt cttcccacgt
     2401 agcgatcggc cgctcatcaa catgattcat gtttcctgtg gcatctcaat tctcgtgctg
     2461 atggtggttc gtcttttgtt aagactgaaa tacccaaccc cgccgattat acctaagccg
     2521 aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct cctgtttatt
     2581 gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg gtttgcgttt
     2641 ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag cttaaagtcg
     2701 tggcatgaga cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc tgcggctgca
     2761 ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc gcgtaaacgt
     2821 tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta caggcgaaga
     2881 atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata aaaccataat
     2941 ccttgcccat acgtccatct ggcttatttt taatcaattc acccgttctt tgatctcatc
     3001 aacggtatca aaataaacca gcgtataacg ttcaaatcgc acataagatt cgataaacca
     3061 tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg tttcctttta
     3121 tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac actgtacggc
     3181 aggcggcaaa tttgtctgta atgatggttc tattagtgca tcgaaaaaaa catgcactaa
     3241 ctgaagtgta aaaggggtgc catgagaaaa tgaatctgct agtcagatgc gcggggaaaa
     3301 tccccgcgct tgcccttacc tggacgtgca ggccatgagc gcagcaacct ccttatcacc
     3361 gtcccggaac cgcagttcgt agagcgtttg tcgggtcagg agtgtgaatg ccagaatcgt
     3421 aatgcagata atgagcagac acaccaccag agggttgcgc ttcatagcct ttctccttgc
     3481 cggatggcgg gtaagaggct aacatctgaa ttgcttggtt cattcgttgg cctcggtttg
     3541 tactgacccc aaaaagttgg acagttaaac acgaggcata taggtctgat tccgatattc
     3601 atttggagtc agacctttta atttcaggct aattcttctg ctgttgtagt attcaatata
     3661 ttccgtaaca gcatccttca gttcgcttat attactgaac tcatcaagat aaaaacactc
     3721 cgactttaag gttccaaaga aacactccac cacagcatta tccagacaat tgccttttct
//
LOCUS       DADLSE010000249         3728 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLSE010000249 DADLSE010000000
VERSION     DADLSE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.251"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(9..389)
                     /gene="panD"
                     /locus_tag="JN692_004073"
     CDS             complement(9..389)
                     /gene="panD"
                     /locus_tag="JN692_004073"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7266197.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERSSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            663..1565
                     /locus_tag="JN692_004074"
     CDS             663..1565
                     /locus_tag="JN692_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7266198.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(1639..2490)
                     /gene="panC"
                     /locus_tag="JN692_004075"
     CDS             complement(1639..2490)
                     /gene="panC"
                     /locus_tag="JN692_004075"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7266199.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2502..3296)
                     /gene="panB"
                     /locus_tag="JN692_004076"
     CDS             complement(2502..3296)
                     /gene="panB"
                     /locus_tag="JN692_004076"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7266200.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     ERLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3409..>3728)
                     /locus_tag="JN692_004077"
     CDS             complement(3409..>3728)
                     /locus_tag="JN692_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266201.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
BASE COUNT          899 a          917 c          963 g          949 t
ORIGIN      
        1 gataacaatc aagcaacctg taccggaatc gctttcgcgg tacgtttcat ttcattgtcg
       61 ccttcaaaat aggcgacatt gggtcgccag gtgcgagctt cttcatctgg catggtaacg
      121 aagctggcga tgatgacaat atcgccgacg ctggcgcagt gggccgccgc accgttaaca
      181 gaaataattc tcgaactgcg ttctgccgcg atggcataag tggagaaacg cttgccgttg
      241 gtgacattcc agatatcgat ggcttcgttt tcgagaatac cggctgcgtc aagaaaatcc
      301 tggtcaatgg cgcaagaacc ttcatagtgc aggtccgcat gagtcacttt cacgcggtgg
      361 agtttgccct gcagcatcgt gcgaatcata acttctacct ttctaccctg tcgttaacga
      421 agcaggcggt gcctgctttg aggaaattct cacgcagtat tgcccgattt ttgatgagtg
      481 tctactcatc tgatggcatt tgcgtcagca gtttgcgtac cgcgccgaag cgtgccatgt
      541 aacgcctgta acgaattgat atttttatga acgctgctcg gtaaacaata aagaggtggc
      601 tgacgatagc acgccttgac tggatactgc cttcacgcaa ggccacacgg aagacaggga
      661 agatggatgc accgagtacc acaccgcatg acgcggtatt taaacaattt ttaatgcatg
      721 cggagacggc tcgcgacttt ctggagatac atttgccagt ggaattacgc gaactttgtg
      781 atctcaacac gcttcattta gagtcgggga gtttcattga agagagcctg aaaggtcaca
      841 gcacggacgt gctctattcc gtgcaaatgc agggcaatcc cggttatctg catgttgtga
      901 ttgaacacca aagcaagccg gataagaaaa tggcctttcg catgatgcgt tattctattg
      961 ctgccatgca ccggcatctg gaggctggcc acgataagct gccgctggtg gtgccgatac
     1021 tgttttatca gggcgaggcc acaccttatc cgctctcaat gtgctggttt gatatgtttt
     1081 actcgccgga gctggcgcga cgcgtctata acagtccttt cccgctggtg gatatcacca
     1141 tcacaccgga tgacgaaatc atgcaacatc ggcggattgc gattctcgaa ctactgcaaa
     1201 aacatattcg ccagcgcgac ttaatgttat tgctggagca actggtcacg ctgattgacg
     1261 aagggtacac tagcggaagt cagttagttg ccatgcaaaa ctatatgctg caacgcggtc
     1321 atactgaaca agcggatttg ttttatggtg tgctgagaga cagggaaacg ggagggaagt
     1381 ctatgatgac gctggcgcag tggtttgaag agaaagggat tgagaagggg attcagcagg
     1441 gaagacagga ggaaaggcaa gaattcgccc tgcgtcttct aagtaaagga atgtctcggg
     1501 aagacgttgc agagatggca aatttacctc ttgctgagat tgataaggta attaacctta
     1561 tttaagttac ctgtgttatg acagatgacg tggggtaaat taataactgg cgccatcagc
     1621 cgtagcgcca gttaagtatt acgccagctc gaccattttg ttgtcgatca ggcgagcatc
     1681 gccaagccag gcggctacca gaattactgc ccgtttgctg gtttcagaaa cttccagcaa
     1741 tgtgtcggca tcgcgaatct gaatatcatc ggcgcggaag cctttttcat tcagttcttg
     1801 ccccgcaatg gtaataattt catcgagatc ccgttcccca gcctgcaatt tgtcagcaat
     1861 cgaacttaaa actttgtaca gaccaggcgc aattttgcgt tgttccgccg tcagataacc
     1921 gttacgggaa cttagcgcca gaccgtcttt ggcgcgcata attggcacac cgacaatctc
     1981 aatatcgaag cccatatcgg caaccatttt gcggatcaac gccagttgct gaaaatcttt
     2041 ttcaccgaag caggcgatgt ccggctggac caggttgaac agcttgctga caatagtcga
     2101 aacgccgcga aaatgtcccg gacggctggc accttccagc atggtcgaaa ggccaggaac
     2161 gtcaacgtaa gtgtgggttt cagtaccgtt cgggtagatc tcttttaccg aaggggcgaa
     2221 aactaaatcc actttacgtt tgtttagctt ctcgcagtcc tcctgcaagg tccgtggata
     2281 acgtgccaga tcttccgggc ggtcgaactg catcgggtta acgaaaatac tgacgacgac
     2341 cacatcggcg cgggctttgg cttcgtcgac cagcttcata tggccatcgt gcagattacc
     2401 catagtaggc accagcgcca cgcgcttgcc ttccatacgc aggcggcgaa tttgctgacg
     2461 cagcagcggc agggtttcga taattaacac aacgtgactc cttaatggaa actgtgttct
     2521 tcgcccggat aaacgccgga ctccacttca gccatatact gccgcacagc cgcgcggatg
     2581 tcgcccgttt cggcgaggaa atttttagcg aatttaggaa tgtgaccgcc ggtaatacca
     2641 aaggcgtcgt gcatcacgag gatttgcccg tcagtgacgt tgcctgcacc aataccaata
     2701 accgggatcg ccagtgcttc ggtaatacgt tttgccagct caaccggcac gcattccagc
     2761 accagcagct gtgcccccgc ggcttctaag gctaatgcat cgctgagtag tcgctcgctc
     2821 gcttcattgc cgcgcccctg tactttgtag ccaccgaaaa tattcactga ctgtggggtt
     2881 aaacctaagt gaccacatac aggaacggca cgttcggtca gcatttttac ggtttcgacc
     2941 agccactcac cgccctcaat tttgaccata ttggcaccgg cacgcataac cgttgcggcg
     3001 ttttcaaagg cttgttccgg cgtggcatac gccataaacg gcaggtcagc caatagcagg
     3061 cagtttggtg cgccgcgacg tacggcggca gtgtggtagg cgatatcagc aacggttact
     3121 ggcagagtgg aatcgtggcc ctgaacggtc atgcccagcg agtcgcctac cagcatgacg
     3181 ttaagccctt catcagcaaa tagtttcgcg aagctgtagt cgtaagcggt gatggtcgcg
     3241 aaacgctttt tatcctgctt atatttctga agtgaagcaa tggtggtcgg tttcataacg
     3301 tatcctgata aatgatgtcg tgttgcctgg cattttatca gtcacattgg tgggggcaat
     3361 gatttatcaa ttaccgcacc gccagatgat ggcggtgcgg tggacttatt aagggtaggt
     3421 tacctggaaa gtactggtgg ctttaaactc accagcttct attggtatag ttccatcctg
     3481 ttgtagcgtg gcctggaaat ggagagggta tgttatacct gtatcttgat ccgggtaaac
     3541 tcctcctgtt gtatcgtctt tgcctctcgg gtcgtaatct ttataaacag aatttgaatc
     3601 attaggtttc aatgtcatta gaggattttt actagttgct aaaccttcta tgagtacacc
     3661 gactcctttt gctgcgtcat tacctgtgag agtattacca aggagttgcc cgttttcagt
     3721 accaacct
//
LOCUS       DADLSE010000250         3625 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLSE010000250 DADLSE010000000
VERSION     DADLSE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.252"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            77..904
                     /gene="nadE"
                     /locus_tag="JN692_004078"
     CDS             77..904
                     /gene="nadE"
                     /locus_tag="JN692_004078"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7266202.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVAYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            1134..2021
                     /gene="cho"
                     /locus_tag="JN692_004079"
     CDS             1134..2021
                     /gene="cho"
                     /locus_tag="JN692_004079"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7266203.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN692_004080"
     CDS             complement(1981..2556)
                     /gene="ves"
                     /locus_tag="JN692_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7266204.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGELSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN692_004081"
     CDS             complement(2759..3244)
                     /gene="spy"
                     /locus_tag="JN692_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7266205.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEECRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT          920 a          858 c          897 g          950 t
ORIGIN      
        1 cccgctgtcg cttgtcgttt cagtagcaac gggttagctt taaggaagtt ttgtcttttc
       61 tgtctggagg ggttcaatga cattgcaaca acaaataata aaggcgctgg gcgcaaaacc
      121 gcagattaat gctgaagagg aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca
      181 aacttatccg ttcattaaat cactggtgct cgggatcagc ggcggtcagg actccacgct
      241 tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc caggaaactg gcaacgaatc
      301 actgcaattt attgccgtac gcctgcccta tggtgttcag gctgacgaac aagattgcca
      361 ggatgccatt gcctttattc aaccggatcg cgtattaacc gttaatatca agggcgcggt
      421 attggctagc gagcaggcat tgcgggaagc aggcattgaa ctgagcgatt ttgtccgtgg
      481 caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc attgcgggta tgaccagcgg
      541 tgtcgtggtg ggcaccgatc atgcagcaga agccattacc ggattcttca ctaaatatgg
      601 tgacggcggt acggatatta atccgctgta tcgtctcaac aaacgccagg gtaaacagtt
      661 actgacggca ttaggttgcc cggaacacct ttataagaaa gccccaactg ccgatctgga
      721 agatgatcgc ccttctctgc cagatgaagt ggcactcggc gtggcctatg acaatatcga
      781 cgactatctg gaagggaaaa acctacctga acaggtcgcc agaacaatag agaactggta
      841 tctgaaaacc gaacataaac gccgtccgcc aattaacgtt ttcgatgatt tctggaaaaa
      901 gtaataattt gcacctattg gattgtgcga aaaagagtaa tttgttcacg ccggatgcgg
      961 cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca atacattgca ggagtcgaat
     1021 aggcctgaca ggcgtagcac gtcagacggt gtaacctttg tcatcaaccc gcctcttttt
     1081 taatcgcctc ccgcctgtta cactggatag ataaccagca ttcggagtca acagtggtac
     1141 ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc aatttatgaa tatcccgaac
     1201 atttacattc attccttaat gacttaccca cccgaccagg ggtgtatctg tttcatggcg
     1261 aaagtgacac catgccgctc tatatcggca aaagcgttaa tatccgcagt cgggtgcttt
     1321 ctcatttacg caccccggat gaagccgcca tgctacggca atcccggcga attagctgga
     1381 tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc gcgattaatc aaagaacaac
     1441 agccgctgtt taataaacgg ttgcgccgca atcgccagct ctgtgccctg caattaaatg
     1501 aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt ttcacgagcc cccaacctgt
     1561 ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt gcagagcatc gctgatgaac
     1621 aaaaactttg ttatggcctg ttgggactgg aaccgttaag tcgcggtcgt gcatgttttc
     1681 gttcagcgct aaaacgttgc gccggagcat gctgcggtaa agagagccat gaggaacatg
     1741 cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt gtgttggcct tggcaaggtg
     1801 cggtggcact gaaagaacag cacccggaaa tgactcaata tcatattatt caaaactggc
     1861 tgtggctggg ggcggttaat tcgctggaag aagcgacaac gttaattcgg acacccgccg
     1921 ggtttgatca cgacggttat aaaattcttt gtaagccgct gctttccggt aactatgaaa
     1981 ttactgaact tgatccggcg aatgaccagc gagccagttg atttcactga acaacagttt
     2041 accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg aaccagcacg cgccctggtc
     2101 ggtagtgagc agtttgtcgc ccagttgcca cgccccgtta atcacaaaca ccaccccgcc
     2161 gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg actttcgcct tacagacatc
     2221 caggcgcgtc ataatattga aatccatcga catctgccct gccgtcagtt tcgcttttac
     2281 cacctggtcc gccgtaaagg caaaaggctg taacggtttt aaggtatggt taaagcgatc
     2341 tgcgctttca aggagcatct cgccgccttc cagcaacgtc actatccttt ccatgccggg
     2401 aaacaaagaa agctccccat tcgccgcgat ggatgcaatg ctggcacgcc agtaaaaatc
     2461 acgttttgcc ggagggaaag tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag
     2521 attcaccgac attttacgca tatcaaagta ttccatactc gctcccggcc cgttcttttc
     2581 attgttatcg ttcaggtaac cgtggatggt tatcggcaac tcgggtgatt tacgttagtg
     2641 gtgatcaggc tttttttaca actgtttgag aagagaaaag aaaaccgccg atcctgtcca
     2701 ccgcattact gcaaggtagt ggacaagacc ggcggtctta agttttttgg ctgaaagatt
     2761 attcagcagt tgcaggcatt ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa
     2821 aattagcatt aaattgcttt ttctgttccg gcgtcagaat gttgtaaatt ttgttctggg
     2881 tttccatgtg cgccagcatg ttagctttgc gctgttcttc catttttgcg atctgcgctt
     2941 cagcttttgc tttatcgaag gtatcgctgg caatgatgtc atgcattgcg cggcattctt
     3001 ccagcggcgg acgtttcatc tggtcacgct ggcctttcat gatttcgtgg atctgctgtt
     3061 tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc ctgatgcgga ccgaacttgc
     3121 ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc ggtagtggtg tctgcggcat
     3181 gggccaagtt agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac
     3241 gcatattcta tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg
     3301 ttgacgagat taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg
     3361 aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt
     3421 gatgagagaa tattacaaca cgatgatttt gcagagatta tgaagaacta taccggatga
     3481 ctggtgataa ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact
     3541 taatacccgc agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc
     3601 cgcgcgtaat cccaacgcta ccaac
//
LOCUS       DADLSE010000251         3614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLSE010000251 DADLSE010000000
VERSION     DADLSE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.253"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..796
                     /locus_tag="JN692_004082"
     CDS             <1..796
                     /locus_tag="JN692_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7266206.1"
                     /translation="WLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            807..1784
                     /locus_tag="JN692_004083"
     CDS             807..1784
                     /locus_tag="JN692_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266207.1"
                     /translation="MKRAPLITGLLLISTSCAYASTLSSTNWQYACSCSAGKAVKLVY
                     MVSPVLTTTGHQTGYYKLNDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYS
                     AATQTGVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGS
                     PSTGDFHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVD
                     FDITYDCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPNVGIRIENLGGGVA
                     NIPFQNGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN692_004084"
     CDS             complement(1827..2687)
                     /gene="rsmI"
                     /locus_tag="JN692_004084"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7266208.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGALLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2752..>3614
                     /locus_tag="JN692_004085"
     CDS             2752..>3614
                     /locus_tag="JN692_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7266209.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGT"
BASE COUNT          923 a          937 c          884 g          870 t
ORIGIN      
        1 ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc gcccaaccca
       61 acatgaaatg cgtcttgatg gctcactgct ggatgatggt cgcctgagct atagcctgga
      121 acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt accgttcacc
      181 ttatggaacc ttcagtgccg gatacagtta cggtaatgac agcagccaat acaattacgg
      241 cgttaccggc ggcgtggtta tccatcctca tggcgtgacg ctctcgcaat atctgggcaa
      301 cgcttttgcg cttatcgatg ctaacggggc ttctggcgtg aggatacaaa actatccggg
      361 gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt atcaggaaaa
      421 ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgaac aaacaacaca
      481 gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca atatcggtta
      541 tcgcgtactt gttacagtca gcgatcgcaa cggtaaaccg ttgccctttg gcgctcttgc
      601 cagcaacgat gatacggggc aacaaagtat cgtcgatgag ggcggcatac tatatctctc
      661 tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg cagatcaaca
      721 atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat tacaaggcac
      781 agcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca ggacttttgt
      841 tgatatccac atcctgcgct tatgcctcaa cgctaagtag cactaactgg caatacgcct
      901 gttcctgctc tgcgggtaag gcagttaaac ttgtctatat ggtcagcccc gtacttacca
      961 ccactggaca tcagacagga tattacaaac tcaatgacag cctggatatt aaaaccacat
     1021 tacaggcaaa cgacattcca ggactcacaa ccgaccaggt tgtctctgtt aacacccgat
     1081 tcacacagat aaaaagcagc accgtatatt ctgctgcaac ccaaacgggt gtttgccagg
     1141 gtgacacgtc tcgttatgga cccgttaata ttggtgcaaa taccaccttt accctgtatg
     1201 tcaccaagcc atttctcggc tcgatgacca ttccgaaaac ggatattgcc gtcattaaag
     1261 gcgcgtgggt cgatggaatg ggaagcccgt ctacaggtga cttccatgat ttagtcaagt
     1321 tatcgattca gggaaatctc accgccccac agtcgtgcaa aattaatcag ggcgatgtta
     1381 ttaaagttaa ttttggattc atcaatggtc agaagtttac cacccgcaat gccatgccag
     1441 acggttttac tccagtagac tttgatatca cttatgactg tggtgatact tcaaagatta
     1501 aaaactcgtt gcaaatgcgc attgacggta caactggggt agtagaccag tacaacctgg
     1561 tcgccaggcg aagaagttca gacaatgtgc ccaatgtcgg tattcgtatt gaaaatctcg
     1621 gcggcggagt tgcaaatatt ccttttcaga acggtatcct tcccgttgat ccttccgggc
     1681 atggcaccgt caatatgcgc gcctggccag ttaatctggt cggtggtgag ctggaaacag
     1741 gaaaatttca gggcacagcc accattaccg tcatggtgcg gtaaaagaag acgttgcgtt
     1801 ttgattaacg caacgcttcg gcactgttac ccctgctgct ccagcgcata cttatacagc
     1861 gcatttttct tcacgccatg aatttctgcg gccagcgccg ccgctttttt cagcggcagt
     1921 tctgcctgta gcagcgccag cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct
     1981 ttatgacctt cgacaatcag caccatctcg cctttgcgac ggttttcatc ttcctttacc
     2041 cacgccagca gcgcgccaac gggcgcgccg tgaatggttt cccaggtttt ggtcagctca
     2101 cgcgccagaa ccacgtagcg ggattcgcct aataccgcaa cgatatcttc caggctatct
     2161 aacagacggt gggtagattc ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt
     2221 agcgcatcac ggcggccttt tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca
     2281 gatggtaaac ccgcggcgct taacgcagtg atagcagcac acggtccggg tagcggcacc
     2341 acacgaatcc ccgcttcacg gcaggtacgc accagatggt agccaggatc gttaattagc
     2401 ggcgttccgg catcggaaac cagcgcaatg ttttgcccct cttgcagctt cgccagcagc
     2461 gtttcggctt tttgttgttc gttatggtcg tgcagcgcaa acaaccgggc attaatccca
     2521 aaatgttgca gcaataaacc ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc
     2581 tgtaatacct ctaacgtacg ctgggtgata tccgccagat tgccgattgg cgtcggtaca
     2641 atgtaaagct ggccctgaga attatccgcc gattggtgtt gtttcattgt gtcgtccgta
     2701 ttgccgattt aatattgagc attgcgtaaa aaaaatatca ctggatacat tatggtaccc
     2761 tcaacatttt ctcgtttgaa agccgcgcgt tgtctgcctg ttgttctggc agccctgatt
     2821 ttcgccggtt gtggcaccca tactcccgat cagtccactg cttatatgca gggcacggcg
     2881 caggctgatt ctgcctttta tcttcagcag atgcagcaaa gctctgatga taccaggatc
     2941 aactggcaat tactcgccat tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt
     3001 gagttgttta accaactacc gcaagaactg aacgattctc agcgtcgcga gaaaacactg
     3061 ctggcggcag agattaaact ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg
     3121 aaaatcacac ctgccgattt agaacaaaac cagcaagcgc gttactggca ggcaaaaatc
     3181 gatgccagcc aggggcgtcc ttccattgat ttactgcgcg cgttaattgc tcaggaaccg
     3241 ctgcttggcg cgaaagaaaa acagcagaat attgatgcca cctggcaggc gctctcctcc
     3301 atgactcagg aacaggcgaa tacgctggtg atcaacgccg acgaaaatat tctgcaaggc
     3361 tggctggatc tgcagcgcgt ctggtttgat aaccgtaacg atcccgacat gatgaaagcc
     3421 gggatcgccg actggcagaa acgttatccg aacaatccgg gcgcgaaaat gctgccaacg
     3481 cagttggtta acgtaaaagc gtttaaacca gcctcgacca acaaaatcgc cctgctgttg
     3541 ccgctgaatg gccaggcagc ggtatttggt cgcactattc agcaaggctt tgaagccgcg
     3601 aaaaatatcg gcac
//
LOCUS       DADLSE010000252         3588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLSE010000252 DADLSE010000000
VERSION     DADLSE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.254"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..372
                     /locus_tag="JN692_004086"
                     /pseudo
     CDS             1..372
                     /locus_tag="JN692_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427357.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            381..809
                     /locus_tag="JN692_004087"
                     /pseudo
     CDS             381..809
                     /locus_tag="JN692_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            870..3293
                     /locus_tag="JN692_004088"
     CDS             870..3293
                     /locus_tag="JN692_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HAY7266210.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKTVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAI
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTKMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPIPAYTSGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            3304..>3588
                     /locus_tag="JN692_004089"
     CDS             3304..>3588
                     /locus_tag="JN692_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7266211.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT          984 a          903 c          898 g          803 t
ORIGIN      
        1 tcattttcct ccagccagtc accagcgaaa aattcgaacg taaaccgatt tactggatcc
       61 tgagtgaagt cgcgaaacgt cttggacctg acgtctatca aaagttcaca gaaggtcgca
      121 cgcaggaaca atggttacaa catctgtacg ccaaaatgct tgccaaagat ccggcgttac
      181 cgtcttacga cgaactgaaa aaaatgggta tctataagcg taaagatccc aatggccatt
      241 ttgtcgccta caaagcattt cgtgacgacc ccgaggcaaa tccacttaaa acgccttccg
      301 gtaagattga aatttattcc agcaagctgg cggaaattgc ccgtacctgg gaactggaaa
      361 aagatgaagt gataagccca ctgccagttt atgcctcaac ctttgaaggc tgggactccc
      421 ctgaacgtag aaccttccca ctgcaactgt ttggtttcca ttacaaatcc cgtactcact
      481 cgacctacgg caacattgat gtcctgaagg cagcctgccg tcaggaggtg tggatcaacc
      541 ctatagatgc acagaaacgt gggattgcca acggcgatat ggtgcggttg tttaaccatc
      601 gtggcgaagt tcggctacca gccaaagtaa caccacgcat tctccctgga gttagcgcta
      661 tgggccaggg agcctagcac gagcccaata tgtctggcga taaaatcgac catggcggat
      721 gtgtgaatac gctaaccact ctgcgccctt caccactggc gaagggaaac ccgcagcaca
      781 ctaatctggt cgagatcgaa aaaatataac ccactacaac cataaattct ggcatgacat
      841 tttgtttgaa aagcaataag tgagtaatga tgaaaatcca taccacagag gcgctcatga
      901 aggctgaaat cagccgtaga agtctgatga aaacctccgc acttggcagt ctcgcgctgg
      961 caagcagtgc tttcactctg ccattttccc aaatggtccg ggcggcagag gctccggtag
     1021 aagagaaaac ggtctggagt tcctgcaccg ttaactgcgg gagccgctgt ctgctacgtt
     1081 tgcatgtgaa agatgacacc gtgtactggg tggagtctga tacgacaggt gacgacgttt
     1141 acggtaatca tcaggttcgg gcatgtttac gcgggcgctc tatccgccga cggatgaatc
     1201 atcctgacag gttgaaatat cccatgaagc gcgtcggcaa gcgcggtgaa ggtaaatttg
     1261 aacggataag ttgggacgaa gccctagata ccatcagtga taatcttcgg cggatcctga
     1321 aagattacgg caatgaggct gtacatgtcc tgtacggaac aggcgtagat ggcggaaata
     1381 tcaccaactc gaacgtcccg taccgtctga tgaactcttg cgggggtttt ctcagtcgct
     1441 atggcagcta cagtaccgca cagatcagtg ccgcaatgag ttatatgttc ggtgccaatg
     1501 atggcaacag cccggatgat atcgccaata cgaaactggt cgttatgttc ggaaataacc
     1561 cggcagaaac gcggatgagc ggcggtggtg tcacttacta cgtcgagcaa gcccgcgaac
     1621 gttcaaacgc acgcatgatc gtcatcgatc cacgctataa cgacacagct gccgggcgtg
     1681 aagatgaatg gctgcccatt cgccctggca ccgatggcgc actggcctgt gcgattgcct
     1741 gggtactgat tactgaaaac atggtcgatc agccatttct cgacaaatat tgtgttggtt
     1801 acgatgaaaa aacgctgccc gccaacgcac cacgtaacgc gcattataaa gcctatattc
     1861 tgggcgaagg gcctgacggc atagctaaaa cgccggaatg ggcagcaaaa atcaccagca
     1921 tcccggcaga aaaaattatc cagttggcac gagagatcgg ttcagcaaaa cctgcttata
     1981 tttgtcaggg ttgggggcca caacgacact ccaacggcga acaaacgtcc cgcgctattg
     2041 ccatactttc cgttcttacc ggtaacgtcg gcataaacgg cggcaactca ggtgtacgcg
     2101 aaggtagctg ggatctgggg gtagaatggt tcccgatgct cgagaatcct gttaaaacgc
     2161 agatttccgt ttttacatgg acagatgcca tcgaccatgg tacgaaaatg accgcgaccc
     2221 gtgatggtgt tcgtggaaaa gaaaaactgg atgtccccat caagttttta tggtgctacg
     2281 ccagtaacac attgatcaat caacatggcg acatcaatca cacccatgag gtgcttcagg
     2341 atgacagcaa gtgcgagatg attgttggca ttgaccactt catgacggcc tcggctaagt
     2401 attgcgatat cctgttgcct gacctgatgc caacagagca agaagacctt atctctcatg
     2461 aatctgcagg gaatatgggc tatgtgatcc tcgcccaacc cgcaacctca gcaaaatttg
     2521 aacgcaaacc catctactgg atgctgagtg aagtcgccaa acgcttagga ccagacgttt
     2581 atcaaacctt tactgaaggt cgcagtcagc atgaatggat caaatatctc catgcgaaaa
     2641 cgaaggaacg taaccctgag atgcccgact acgaggagat gaaaacgacc gggatcttta
     2701 agaaaaaatg cccggaagaa cactacgtcg ctttccgcgc attccgtgaa gatccacagg
     2761 caaacccgtt gaaaacacct tcggggaaaa tcgaaattta ttctgaacga ctggcgaaga
     2821 ttgcagatac ctgggaattg aaaaaagatg aaattattca tcccattcct gcgtatacct
     2881 caggttttga tggctgggac gatcccctgc ggaaaaccta tccactgcag ttaacgggct
     2941 tccactacaa agcgcgtacc cactccagct acggcaatat tgatgtgtta cagcaggcct
     3001 gcccacaaga ggtgtggatc aaccccattg atgctcaggc acgcggtatc cgtcatggcg
     3061 ataccgtgcg ggtatttaac aataatggag aaatgctgat tgccgcaaaa gtgactccgc
     3121 gtattctgcc tggcgttacc gccatcgggc aaggtgcgtg gcttaaagcg gatatgtttg
     3181 gtgaccgggt cgatcacggc ggcagtatca atattctgac ctctcaccgc ccttcaccgc
     3241 tggcaaaggg aaacccgtcg cacagcaatc ttgtccagat cgaaaaggtt taaggagtag
     3301 cccatgacca cacaatatgg attttttatc gattccagcc gctgtactgg ctgtaaaacc
     3361 tgcgaactgg cgtgcaaaga ttttaaagat cttggcccgg aagtcagttt ccgccgtatt
     3421 tatgaatatg ctggcggcga ctggcaggaa gataatggca tctggcacca gaacgtgttc
     3481 gcttattatc tctccatctc ctgtaaccat tgcgatgacc ccgcatgtac aaaagtctgc
     3541 cccagcggcg caatgcataa gcgtgaagat ggctttgtgg tggttgat
//
LOCUS       DADLSE010000253         3586 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLSE010000253 DADLSE010000000
VERSION     DADLSE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.255"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..248
                     /locus_tag="JN692_004090"
                     /pseudo
     CDS             1..248
                     /locus_tag="JN692_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            272..679
                     /locus_tag="JN692_004091"
                     /pseudo
     CDS             272..679
                     /locus_tag="JN692_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1264..1980
                     /locus_tag="JN692_004092"
     CDS             1264..1980
                     /locus_tag="JN692_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266212.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGSYEINDVYVRK"
     gene            2532..2936
                     /locus_tag="JN692_004093"
     CDS             2532..2936
                     /locus_tag="JN692_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266213.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2999..3298
                     /locus_tag="JN692_004094"
     CDS             2999..3298
                     /locus_tag="JN692_004094"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266214.1"
                     /translation="MTKTVSTSKKTRKQHSPEFCSEALKLAERIGVAAAARELSLYES
                     QLYAWRSKLQQQMTSSERESELAAENARLKRQLAEQAEELAILQKAATYFAKRLK"
     gene            3295..>3586
                     /locus_tag="JN692_004095"
     CDS             3295..>3586
                     /locus_tag="JN692_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266215.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRA"
BASE COUNT         1011 a          743 c          847 g          985 t
ORIGIN      
        1 actggcagag atagctgtcg ctggcggcca acagtgacgt ccccatgatg aagaatgtgg
       61 ctaaaacgat agggaaaagg ttgtacggta tcctgaacgc catacgccat ggcgtatcaa
      121 acggaaatgc ggaagcactg aacagcaaga tcaggctgct gagaataaaa gccaagggct
      181 accgcaaccg ggagcgcttt aagctgggag tgatgttcca ctatgggaaa ctgaatatgg
      241 cgttctaagc cttcccacca tgatcgggga agacccagat atgaacaggc acattcagag
      301 ttactttaaa gagcttactg aacgtctgag gtgttagagc catgactgcc gcagcattgc
      361 tggcggaaat gcctgaatcg agcagcctct cgagacgtga aatcagcgca cttgtcggtg
      421 ttgctcaggt aaaccgggac tcgggaactc tgcgcggtcg acgaaccatt ttcggtgatt
      481 gtgctgggga agaacagctc tgtacatggc ggcgcttgag gcaccccggt ttaatctggt
      541 gataaaggcg ttttacatgc gcctacttgc agccggaaac gccaaaaaag tggcactggt
      601 tgcctgtatg cgtaagttgc tgaccattat gaacgcaatg ctcagaaaaa acgaagaatg
      661 gaatgaatcg tatttatgaa aactggattg tgggccataa atattccaaa aaaatagccc
      721 gctccgttta ttaccggaac gggcgagagc atgacattgt ctgtaattat aatgagactg
      781 ctgattctac ttatttttaa tttcaagaaa agcgttttct acaaagaatt atgagattta
      841 atgttttgtt tagacaatat tatcattctg ttcaataatg aaagtcttca tcaacaacaa
      901 gtaaaaacaa cgtccctctc atcgaaccgg acttgcagat ttcccgcatc cggctctctt
      961 gcaacacatc agaccttcac gttcgggcgg ttatggggaa ggtccagcaa gctgtatgag
     1021 ccctaactgt ttgaatacca gtagattacc agaaacttct atcgattgaa gacattacca
     1081 actagcgtta ctaaatgttg accaagtttt gttcacttgt ctggttttga tagtagcatt
     1141 ctgcagagta ttttgatatg ataaatatgc attaacaatg atattattgt taatttccta
     1201 gcatgaaaat taaaagatca actttttatc tcgaatattt tttacattat cagttggttt
     1261 cttatgaatg acaattcatt gcttagaaat tcttcattat tcatcgccta tatgggatgt
     1321 gtagggtggg tcagtgctta ttcttatggc tggggaacct ctttttacta tggatttcca
     1381 tggtgggttg ttggtgcagg tttggatgat gttgccagaa gtttactcta tgctatcata
     1441 gtaatgggta tactttttac cggttggggc attggtattt tattttttct tcttataaag
     1501 aaaagaagta aaatacaaga cttgagtttt tttagactat tttttgctat taccctactg
     1561 tttttccctg taatattcga acttttaatt ctcaaacaat atttcatact tccattatca
     1621 ctttcatgca ttattagttc attggttatt tcaataataa ttcgtatata tggacggatc
     1681 ttttctgtat catgtttttc agatatccct tttgttagag aacaccgcat taagttaatt
     1741 atggctggtt ttttggttta tttttggctt ttttcctttc ttgttggctg gtataaacct
     1801 caattaaaaa aagaatatca gatgctttgt tataataata gttggtatta tgttcttgct
     1861 cgttatgatt cacggctcgt tttgtctagt tcatttaaag atgacagtaa cagattctta
     1921 atatttaata cagaacagtc gggatcttat gaaatcaatg atgtttatgt cagaaagtaa
     1981 taatcctctc ccacaaagtg aattaaatga atatataatg cgttcgaagt atatgtattg
     2041 agctgtcata ctgagaaaca aaccttaggg cagcacaatt aaagccagaa acaactatgc
     2101 aagaaagtta ctaaataagt gctcggaaca gtaaaacact caggggggac tcagtccaga
     2161 ttatgtaatc agataaattg ccaaacatca caaatcacca gccggactga gtgatgccga
     2221 gcattgcacc aataccccgt gatgaacgat gcctgatgca gaaagctccc tataaaacaa
     2281 agaacaaaaa ctacgaccgg cgattcaccg tcatactgat gctgcatcgt ggtgacactg
     2341 tctgtcactt tcatgttgac tgaacaactc tgctgcgaag aaggtgtacc gccggcggga
     2401 tttgattgtc cggcgtctag cttccacttc cctgatggaa ctgaaagttg ttatggccgg
     2461 tcagcttgcc ctgtccgcag gacatgaggt gctgtgacgg acagcaccag taacggaaag
     2521 tgacaatgaa aatgaatact gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac
     2581 ccgctgctgc aaaagagact ttactggcta tgccacgtaa ttcaacagaa cattgctatg
     2641 cggagattaa tgtgcacgga ccatatgggg tgtatttccg agtggtcccg catccccccg
     2701 ggggaaagag ctgggtcgaa tgtaacagtg actactatta ctctgataaa ccaccgggag
     2761 tacagatact cggaacccgt gcagggtgtc gggtatacgg aatctgcggg accacctcga
     2821 cactgcatgt ggctggaaga ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga
     2881 tgataattca caggatacgg aaacgtcctg tagtggctgt aagcgatgag atgtgatctt
     2941 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgactcacat
     3001 gacaaaaaca gtatcaacca gcaaaaaaac acgtaaacag cattcacctg aattttgcag
     3061 tgaagccctg aagcttgctg aacgcatcgg tgttgctgcc gcagcccgtg aactcagcct
     3121 gtatgaatct cagctctatg cgtggcgcag caaactgcag caacagatga cctcgtctga
     3181 gcgtgaaagt gaactggccg ctgaaaatgc ccgcctgaag cggcaactgg cggagcaggc
     3241 agaggaactg gctatcctcc aaaaggccgc gacatacttt gcgaagcgcc tgaaatgaag
     3301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aggcaatgtg ccgcgtgctc
     3361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacagggat aagccctcgt
     3421 cagcagttcc gtcagcactg cgacagcgtt gtcctcgcgg cggcctttac ccggtcaaaa
     3481 cagcgttacg gtgcaccacg cctgacggat gaactgcgtg ctcagggtta ccactttaac
     3541 gtaaaaaccg tggcggcaag cctgcgccgt caggggctga gggcaa
//
LOCUS       DADLSE010000254         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLSE010000254 DADLSE010000000
VERSION     DADLSE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.256"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN692_004096"
     CDS             complement(<1..702)
                     /gene="tqsA"
                     /locus_tag="JN692_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7266216.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALN"
     gene            1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN692_004097"
     CDS             1114..1479
                     /gene="mdtJ"
                     /locus_tag="JN692_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7266217.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            1466..1795
                     /gene="mdtI"
                     /locus_tag="JN692_004098"
     CDS             1466..1795
                     /gene="mdtI"
                     /locus_tag="JN692_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7266218.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2655)
                     /locus_tag="JN692_004099"
     CDS             complement(1834..2655)
                     /locus_tag="JN692_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7266219.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(2755..2838)
                     /locus_tag="JN692_004100"
     CDS             complement(2755..2838)
                     /locus_tag="JN692_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266220.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(2931..3266)
                     /locus_tag="JN692_004101"
     CDS             complement(2931..3266)
                     /locus_tag="JN692_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7266221.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT          904 a          831 c          857 g          951 t
ORIGIN      
        1 attaagcgca aaggccagca atccccagac aaaagcgaag cgaacatcga gtgcggcgag
       61 catcgcccag gcgaccaggc cggtgatgat gctgatggct gttttcagca ccagataatg
      121 agaaacactg tcaatcgcac gttgaatcgc cgccatccct tcaaccggac gcgccatcat
      181 ttgctgaaat tttccgggca attgtggcac ttcgagcagc ataaacagca ccgtcagcag
      241 caataaaaat attgatgaca tggcattaga taactgcgtc aataagttgg tgagcaacgt
      301 catcgccgcg ttcggatcaa tgtaatgcgc cagttggtca actgagacgt caatccctac
      361 gcgttgcaac aacggctcaa gagcttgcag cggcgtcata atagagttgc gatattgcgg
      421 taacgtccgc gtcaactcgt tgagcgcgga acccagataa gctaatagca acaccatcgc
      481 catcacgatg attgtcatca aaatcgacac tgccagtaca cgcggcacac gccagcggac
      541 catatgttgc accagcgggt taagaataac agcaataaat aatgcgagaa taaacggcac
      601 gatgatctcg gcggcaaaac ggataccgca gagaataatg accagcattc ccaacataat
      661 gacgattttt aggccattga gcgtgatgat cggctttgcc atgcttgtcc ccgttttttc
      721 ttcctttgct aataataatg tctttttcgc tccgtcatca taacgaaaat caaaccgctc
      781 aggtaaagaa gtgaaaatat tttgagttaa ttcttaagct atgatacaaa tcaggcgtgt
      841 tcaactaccg aggacaatta tcatctgcga tgacgagaag caacactgcg gataattgta
      901 atattatgga caatttgttc aggacgttat tttctacatt tacccatctt cgtacctcat
      961 ccactatctt gctcgtaggt gagcagcact ggcgcaacgc gctttagtcc tttcttctgc
     1021 ctaagcttgc cctatgcgca gggcttgacc actttacttt ggtttcgctg aattaagcga
     1081 aaattaaaat agttctcttg caggagaagg acaatgtata tttattggat tttattaggt
     1141 ctggctattg ctacagaaat taccggtacg ctgtcaatga aatgggcgag cgtcagtgag
     1201 ggaaatggcg gctttatttt aatgctggtg atgatttctc tgtcgtatat atttctctct
     1261 ttcgccgtta aaaaaatcgc cttaggcgta gcttatgcgc tgtgggaagg tatcggtatt
     1321 ttatttatta ccttgtttag cgttttgtta ttcgacgaaa gtttatcgct gatgaaaatt
     1381 gccgggttaa ccaccctggt cgccgggata gtgttgatca aatcaggtac ccgtaaagcg
     1441 cgtaaacctg aactggaggt gaaccatggc gcagtttgaa tgggttcacg ccgcctggct
     1501 ggcattggca atcgtgctgg aaatcgttgc taacgtcttt ttgaaatttt ctgacggctt
     1561 tcgtcgcaaa atatttggct tgctctccct ggcggcggtg ctggcggcct ttagtgcgct
     1621 ttctcaggcc gttaaaggga tcgacttgtc tgtcgcttat gcattgtggg gcgggtttgg
     1681 tattgccgcc acgttagccg caggttggat cttgtttggt caacggttaa atcgtaaagg
     1741 ctggattggc ctggtcttgc tgttggctgg aatgatcatg gtgaaacttg cctgatgaag
     1801 acgctgcccg cgctgtcggg cagcgtttga acattatttt tgcgacagtt gatccagctt
     1861 gtcgcgaaaa ccggtaacag aaatggcccg gttatcggcg cgccagcgat ctttcgcggc
     1921 aggagccgaa ctttgcaccc caattaattg ccagccgtca tcggtatgca acatcagagg
     1981 cgaaccgctg tcacccggca aggtatcgca ctgatgtgac atcaccgacg tttgcgccca
     2041 gccagtcact tcacagtttt gatgactgta taacgtatcg agatgatctt cagggtatcc
     2101 cgcctgagtc actttacgac cagccgattt taatgcggca gtaagcgcgg ctttatctcc
     2161 ctcaaataac ggcaacggtg taatgccaga agggggatta cgtagcacaa tcaatccgaa
     2221 gtcccacggt gcagctgcgg gaggaacaat ccaaccatcc ccatctgctt ttaaccgctt
     2281 tcccagtgtc ggatcaacgc ggccttctat gtcgtggatc tcatagcgcc aaagaccttt
     2341 atttgacaca aaacgcagcg ctaccgcctt atccgcttta cccttaggcg gcgtcaataa
     2401 acagtgtcct gccgttaatg ccagattggg tgcaatcagc gtcgccgtac ataaattgcc
     2461 gctggccgtt tccagttgcc caaccgcatc ccacggtgat tgggtcgtgt cattcactgg
     2521 cacacgatca tcatgaccaa aaaacagggt gctgacctca tcgtttgccg atttggcaac
     2581 gtctggttta tctgcaaaca caaaagcaga tgtcaaacta attgcaccca acactacagc
     2641 aatggttgta cgcatatcac actctggtgg gtaattatgc ttattaaaag caaaccctca
     2701 taaatactat agacgggacg ggatgaaagt gggagtaaaa tcagataact acagtcagga
     2761 aagataaaaa cgggcggcaa tcagtgcgca taaaataagg atgataagaa tgaactcaaa
     2821 gcgataacgg cccaccatac taccctccgc taaaggcggt gctaaacgtt acccgcttag
     2881 caccgtaatt ttcaggcgcg agggggcgcg ccagcattac tgttgaaaac ttacgctgcg
     2941 ggttgtgcag caggttttgc cggttgctga tggccgtgtt tcccggcgtg tttcttcgct
     3001 gcctgcgctt tttgttcagg ggctttctgt tcagctttcg tatttttatg atgcttttta
     3061 gccgcctgcg ctttctgggc aggtgctgct ttatgctgtt ttttatgatg tgtagttttc
     3121 gctggcgctg ctttggtggt cgtcgcagtc ggagcaggtg tggtcgcagt ctctgcagca
     3181 aaggcggcag aagacagacc catagcagcg gcaacaacca gagctaatac ttttttcatt
     3241 gtcataccct caatttgttt tttcatttaa ccccactgcg gggccgttga aataactata
     3301 tccctgggaa tggcagactt ccgtgagtgg ttggtttcag cgtgtaacga tatgtacaaa
     3361 cgctgaataa attacagcgt tgataataac gtattgtgac ttaaggaaaa tttagctacc
     3421 aataatagta gtcttgatcg ggttaactat tatactactt acaaagcggt agaataactg
     3481 cgcatcagta ataaatgaca cacagcaaaa tgaatccgtt tatttgggta cttataatcc
     3541 tga
//
LOCUS       DADLSE010000255         3543 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLSE010000255 DADLSE010000000
VERSION     DADLSE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.257"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN692_004102"
     CDS             complement(<1..1031)
                     /gene="curA"
                     /locus_tag="JN692_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7266222.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLAPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGEMLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMVQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
     gene            1212..1730
                     /gene="mddA"
                     /locus_tag="JN692_004103"
     CDS             1212..1730
                     /gene="mddA"
                     /locus_tag="JN692_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7266223.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1727..2176
                     /locus_tag="JN692_004104"
     CDS             1727..2176
                     /locus_tag="JN692_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7266224.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2177..2410)
                     /locus_tag="JN692_004105"
     CDS             complement(2177..2410)
                     /locus_tag="JN692_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HAY7266225.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2496..2669)
                     /locus_tag="JN692_004106"
     CDS             complement(2496..2669)
                     /locus_tag="JN692_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7266226.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2864..2959
                     /gene="yncL"
                     /locus_tag="JN692_004107"
     CDS             2864..2959
                     /gene="yncL"
                     /locus_tag="JN692_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7266227.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(3361..>3543)
                     /locus_tag="JN692_004108"
     CDS             complement(3361..>3543)
                     /locus_tag="JN692_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HAY7266228.1"
                     /translation="VIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQ
                     ERDYKKFNLSEVFRGL"
BASE COUNT          875 a          887 c          803 g          978 t
ORIGIN      
        1 tcacccgcga cgcggatcac cactttgccg aagtttttac ccttcagcag gccgataaac
       61 gtctgtggcg cattctctaa accatcagta atatcttcgc ggtagtggat tttatcctct
      121 ttcacccatt gcaccatctc cttctgaaac tcatggatgc ggtgaccata atcctgagcg
      181 ataataaacc cttgcaagcg aatacgtttt ttcagcaccg ttgccatcaa caggggtaaa
      241 cgatccggac cgggtggtag ctctgtagcg ttatagctgc tcactaagcc acaaacggga
      301 atgcgcgcag atgtattaag taatggtagc actgcatcaa ataccttacc gcccacgttt
      361 tcataataga tatcaatacc ttttgggcac gctttcgcca gttgttcggc aaaatcgtcc
      421 gcgtggtgat caaggcaaac atcgaagcct aacacctcgg tagcatggcg gcatttttcc
      481 gcgccaccgg ctacccccac cactctgcaa cctttaagtt tgccgatttg ccccaccgtc
      541 gcccccactg gtcctgtcgc cgcagctacc accaacattt cgccctcttt aggctgaccg
      601 atatccagca gccccatata agcggtaaag cctggcatcc ccagcacacc cagcgaccac
      661 gatggatttt gcggatgatc gccaagtttc accaggtcat caccactgga tatgtcatag
      721 tcctgccatc cactgtagcc cagcacccag tcgccaggct gataatcagg atgattcgac
      781 tccacgacac ggctcaccgt accgccgacc atcacgccgc caatatcaac cggcggtgaa
      841 taagatggct catcgctcat acgtccacgc atatacgggg ccagggacaa ataaactgtg
      901 cgcagtaaca cctgtccttc acccggtgtg gcgacatcat cttcttcaag acggaaattc
      961 tccggaaccg gtgcgccatg tggacgcgag gccagaaccc aacgtcgatt acgctgcttt
     1021 tgttgcccca taatgctctc ctgtgctttt ggcatgaatc catcaatagt agcaggctca
     1081 cgggattggt tttgtttgca gccgtcacat cccctctgca acacgcaata atttcatcga
     1141 ggaatccgtt gcttcgggct gttttcgata gtattgataa catcatcatc attattttat
     1201 tgaggccaga catgtccatc cgttttgccc gcaaagccga ctgtgctgcc attgcggaaa
     1261 tttataacca cgccgtgttg tatacggcgg ctatctggaa tgaccaaacg gtggatgcag
     1321 ataaccgcat tgcctggttt gaagcgcgga ccatagcagg ttatccggtg ctggtaagtg
     1381 aagaagatgg cgtagttacg ggatatgcct cgtttggcga ctggcgtagt ttcgatggtt
     1441 ttcgccatac cgtggaacat tcggtttatg tccatcccga tcatcagggc aaaggtctgg
     1501 ggcgtaaatt gttaagccga ttgattgatg aagcgcggga ttgcgggaag catgtcatgg
     1561 tcgccgggat cgaatcgcaa aatcaggctt cgctgcatct gcaccagtcg ctgggatttg
     1621 tcgtcaccgc acaaatgccg caggtaggca ctaaatttgg tcgttggctg gatctgacat
     1681 ttatgcagtt gcaactcgac gagcgcactg aaccggacgc gattggatga atcagtcgct
     1741 cacccttgct tttttgattg ccgccggaat tggtctggta gtccagaaca cgctgatggt
     1801 gcgcatcacc cagacctcct ctaccattct catcgccatg ttgctgaact cactggtggg
     1861 gattgtgctc tttgtttcta ttttatggtt taaacaaggc atggcggggt ttggcgaact
     1921 ggtgtccagc gtgcgctggt ggacacttat acctggcttg ctgggttcgt tttttgtctt
     1981 cgccagtatc agcggatatc agaatgtggg agcggcgaca actattgcgg tactggtcgc
     2041 cagccagctg attggcggtc tggtgctgga tatcttccgc agtcatggcg taccactacg
     2101 ggcgttgttc ggcccaatat gtggcgcaat tttacttgtg gttggcgcct ggttggtggc
     2161 cagacgctca ttttaatcag aggatggtgc cgcctttggt taactgttcg tggcgcgctt
     2221 ccatatcttc tttatgcttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct
     2281 gggtgtagcg atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt
     2341 tcatatgggc aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca
     2401 gatgcgacat actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata
     2461 agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc
     2521 accgggaaac tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt
     2581 ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa
     2641 aaaacaagca tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc
     2701 gcgcaaatat gcgtcatttt ttgatgtgca tcaagttttt tcaccagttt gagaaaaaat
     2761 catcaaacag caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta
     2821 acccgtacta tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag
     2881 aactgtagta ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag
     2941 atttggctgg tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca
     3001 actctctgac agtacgcaag atcttctcat actctctagg tttgcaacat tatccttaat
     3061 atttacttct ttttgctggc atctacattg aaatgagtag aaaacatccc cctactgtcg
     3121 tctatacaaa cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa
     3181 tactttatac gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata
     3241 aaaagatggc gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc
     3301 ccattgaaat tgcaaacccc gccctggtta tcttgcccat ttatcatgca aaacagataa
     3361 ttataggcct ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt
     3421 tccatttgat atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt
     3481 attatccgga agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat
     3541 aac
//
LOCUS       DADLSE010000256         3505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLSE010000256 DADLSE010000000
VERSION     DADLSE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.258"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN692_004109"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN692_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7266229.1"
                     /translation="MLNTCRVPLTDRKVKGKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JN692_004110"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JN692_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7266230.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JN692_004111"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JN692_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7266231.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2901..>3505)
                     /locus_tag="JN692_004112"
     CDS             complement(2901..>3505)
                     /locus_tag="JN692_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266232.1"
                     /translation="TIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTNCNS
                     VRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELV
                     FEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYS
                     FQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          851 a          776 c          844 g         1034 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgttaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttcccct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga
      481 ctgacaacaa attaattatt acttttccta attaatccct caggaatcct caccttaagc
      541 tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca
      601 ccgctgatgg cgcaaattct tcaatagctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcgat tctttagcag tgatgcctcg ggaggcatta
      781 ttcttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg gcttttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg
     1141 cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agcgctgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcatttgc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     2101 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     2401 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgacttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa
     2881 gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gataattttc attattgata cgagacagta caaatcgtcc agtgtcgatg
     3301 gattgatctt caaaaccaca ggtcattaaa tttacctgta aaatagtatt taaattaaaa
     3361 ttaaagcgga cgctattaca attagtgtta ttccctgtac atgaactcac ttcaatttcg
     3421 aacaacaaag gaaaaggcgt tcctggaaaa atatctgcgg tgttggaact atctgataca
     3481 gatgcgatag agtatggaat ggtga
//
LOCUS       DADLSE010000257         3493 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLSE010000257 DADLSE010000000
VERSION     DADLSE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.259"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..432
                     /locus_tag="JN692_004113"
     CDS             <1..432
                     /locus_tag="JN692_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266233.1"
                     /translation="PIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGIL
                     QLGVSVMSEFFPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALL
                     ALITAIIVFSRYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            444..1310
                     /gene="ydiB"
                     /locus_tag="JN692_004114"
     CDS             444..1310
                     /gene="ydiB"
                     /locus_tag="JN692_004114"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7266234.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            1341..2099
                     /gene="aroD"
                     /locus_tag="JN692_004115"
     CDS             1341..2099
                     /gene="aroD"
                     /locus_tag="JN692_004115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7266235.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            2245..>3493
                     /locus_tag="JN692_004116"
     CDS             2245..>3493
                     /locus_tag="JN692_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HAY7266236.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLIIALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYAIFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCG"
BASE COUNT          913 a          798 c          874 g          908 t
ORIGIN      
        1 cccatctggg ctaatgtatt taactctgcg ctggcaacaa taacagctgc cattatctac
       61 ctgtaccctt ctccactggt gtgtaatgcc ggcgcctttg ttatcggttt ctcagcagct
      121 ggcggcattt tacagctcgg tgtttcggtc atgtcagagt ttttccccaa aagcaaagcc
      181 aaagtcacca gtatttatat gatgatgggt ggactggcta actttgttat tccgctgatt
      241 accggttatc tgtcgaacat cggcctgcaa tatatcattg ttctcgattt tactttcgcg
      301 ctgctggccc tgattaccgc aattattgtt tttagccgct attaccgcgt ttttattatt
      361 cctgagaatg atgtgcggtt tggcgaacgc aaatttagca cccggttaaa cacaattaag
      421 catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg attggattga
      481 tggcttatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag
      541 cgggattgcc atttacctat atggccttcg aagtggataa cgatagcttt cccggagcaa
      601 ttgaaggatt aaaagccctc aaaatgcgcg gaactggtgt gtcgatgccg aacaaacaac
      661 tggcgtgtga atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca
      721 ccatcgttaa tgatgatggc tatctgcgtg gctataacac cgacggcacg ggccatattc
      781 gcgccattaa agagagcggt tttgatatca aaggcaaaac gatggtgctg ttaggggccg
      841 gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac
      901 tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg
      961 aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc tttgctgaag
     1021 ccctggcttc cgccgacatt ttaaccaatg gcacaaaagt aggtatgaaa ccccttgaga
     1081 atgaatcatt ggttaatgat atcagtctgt tacatccggg actgctggtc actgaatgcg
     1141 tgtataaccc gcatatgacg aagttattgc agcaggcaca acaagctggt tgcaaaacga
     1201 ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca
     1261 aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc
     1321 gcgtgcagaa agggtaaaaa atgaaaaccg taactgtaaa agatctcgtc attggtgcgg
     1381 gcgcacctaa aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg aaatccgaag
     1441 ctctcgccta tcgtgaagcg gactttgata ttctggaatg gcgtgtggac cactttgccg
     1501 acctctccaa tgtggagtct gtcatggcgg cggcaaaaat tctccgtgaa accatgccag
     1561 aaaaaccgct gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag gcgatttcca
     1621 ccgaggctta tattgctctc aatcgtgcag ccatcgacag cggcctggtt gatatgatcg
     1681 atctggagtt atttaccggc gatgatcagg tcaaagaaac cgtcgcctac gcccacgcgc
     1741 atgatgtgaa agttgtcatg tccaaccatg acttccataa aacgccggaa gccgaagaaa
     1801 tcattgcccg tctgcgcaaa atgcagtcct tcgacgccga tattcctaag attgcgctga
     1861 tgccgcaaag taccagcgat gtgctgacgt tgcttgccgc gaccctggag atgcaggagc
     1921 agtatgccga tcgtccaatc atcacgatgt cgatggcaaa aactggcgta atttctcgtc
     1981 tggctggtga agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa gcctctgcgc
     2041 cagggcaaat ctcggtaaat gatttgcgca cggtattaac tattttacat caggcataag
     2101 caataatatt tcggcggaaa taccctcccc cgccgaaata aaaaaagtat ttttaaccgt
     2161 attttataaa aatatttcgt gagtctctgt gcgctaattc tccatctggc gtagggaaaa
     2221 tcacacctga atcaggaatt aacaatgaaa cctgtaaaac cacctcgtat taatggacga
     2281 gtgccggtcc tgtcggcaca ggaagcggtg aattatattc ccgacgaagc aacactttgt
     2341 gtgttaggcg ctggcggcgg tattctggaa gccaccacgt taattattgc tcttgctgat
     2401 aaatataaac agactcaaac gccacgtaat ttatcgatta ttagtccaac agggcttggc
     2461 gatcgcgccg accgcggtat tagtccgctg gcgcaagaag gtctggtgaa atgggcatta
     2521 tgcggtcact ggggacaatc gccgcgtatt tctgatctcg cagaacaaaa taaaattatt
     2581 gcttataact atccacaagg tgtacttaca caaaccttac gcgccgccgc agcccaccag
     2641 cctggtatta ttagtgatat tggcatcggg acatttgtcg atccacgcca gcaaggcggc
     2701 aaactgaatg aagtcactaa agaagacctg attaaactgg tcgagtttga taacaaagaa
     2761 tatctctatt acaaagcgat tgcgccagat atcgccttca ttcgcgctac cacctgcgac
     2821 agcgaaggct acgccatttt tgaagatgag gtgatgtatc tcgacgcatt ggttattgcc
     2881 caggcagtgc acaataacgg cggtattgtg atgatgcagg tgcagaaaat ggttaagaaa
     2941 gccacgctgc atcctaaatc tgtccgtatt ccgggttatc tggtggatat tgtggtggtc
     3001 gatccggatc aaacccaact gtatggcggt gcaccggtta accgctttat ttctggtgac
     3061 ttcacccttg atgacagtac caaacttagc ctgcccctaa accaacgtaa attagttgcg
     3121 cggcgcgcat tattcgaaat gcgtaaaggc gcggtgggga atgtcggcgt cggtattgct
     3181 gacggcattg gcctggtcgc tcgggaagaa ggttgtgctg atgactttat tctgacggta
     3241 gaaacaggtc cgattggcgg aattacttca cagggtatcg cctttggcgc gaacgtgaat
     3301 acccgcgcca ttctggatat gacgtcccag tttgattttt atcacggtgg tggtctggat
     3361 gtttgttatt tgagttttgc tgaagtcgac cagcacggta acgtcggcgt gcataaattc
     3421 aatggcaaaa tcatgggtac cggtggattt attgatatca gtgccacctc gaagaaaatt
     3481 attttctgcg gca
//
LOCUS       DADLSE010000258         3464 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLSE010000258 DADLSE010000000
VERSION     DADLSE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.260"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1981
                     /locus_tag="JN692_004117"
                     /pseudo
     CDS             1..1981
                     /locus_tag="JN692_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1987..2700
                     /gene="ybiX"
                     /locus_tag="JN692_004118"
     CDS             1987..2700
                     /gene="ybiX"
                     /locus_tag="JN692_004118"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7266237.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2774..3040
                     /gene="ybiI"
                     /locus_tag="JN692_004119"
     CDS             2774..3040
                     /gene="ybiI"
                     /locus_tag="JN692_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7266238.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3305..>3464
                     /locus_tag="JN692_004120"
     CDS             3305..>3464
                     /locus_tag="JN692_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266239.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEA"
BASE COUNT          893 a          922 c          869 g          780 t
ORIGIN      
        1 gggtgcgttt tttgcgggtg agaacggtaa ctccaccact ggcgacgcca tttatatgcg
       61 tggtgccgat acctctaaca gtatttatat tgatggtatt cgcgatatcg gcagcgtctc
      121 gcgcgacacc ttcaataccg agcaggtcga agtgattaaa gggccgtccg gcaccgacta
      181 cgggcgcagc gcaccgacag gctcgatcaa tatgatcagc aagcagccgc gcaatgattc
      241 cggcattgac gcctccgcca gtattggcag cgcctggttc cgccgcggca cgctggacgt
      301 caatcaggtc attggtgata ctaccgcggt gcgcctgaat gtgatgggcg aaaaaaccca
      361 cgatgccgga cgcgacaaag tcaaaaatga gcgttacggc gtcgcccctt ctgtcgcttt
      421 tggccttggt acagcgaatc gtttgtatct taattatctg catgtcactc agcacaacac
      481 gccagacggc ggcattccga ccatcggttt gccgggctat tctgccccat ctgcgggagc
      541 ggcggccctg aatcattccg gaaaagttga tactcataac ttttacggca cggattccga
      601 ttacgacgat tcgaccaccg acaccgccac catgcgtttt gagcacgaca tcaacgataa
      661 caccaccatt cgcaatacta cccgttggtc gcgcgtaaag caggattacc tgatgacggc
      721 gattatgggc ggggcgtcga atattactca gcccaccagc gatgtgaata gctggaccta
      781 gtcacgcacg gcgaatacca aagatgtgag taataaaatt ctcaccaacc agaccaacct
      841 gacctcgacg ttctataccg gttctatcgg tcatgatgtc agtaccggcg tggaatttac
      901 ccgtgaaacg cagactaact atggcgttaa tccggtgacg ttaccggcgg taaatattta
      961 tcatcctgac agcagaattc atcccggcgg cctgacgcgc aacggcgcaa acgccaatgg
     1021 tcagacggat accttcgcaa tttacgcctt cgatacgctg caaatcaccc gtgattttga
     1081 gctgaacggc gggatccgtc tggataatta tcatactgaa tatgacagtg ccaccgcctg
     1141 cggcggcagc ggacgcggtg ccatcacctg cccagctggt gtggcaaaag gttctccagt
     1201 caccaccgtc gacaccgcca agtcgggcaa tctggtgaac tggaaagccg gggcgctgta
     1261 tcacctgacg gaaaacggca atgtctatat taactatgcc gtttcccagc agcctccggg
     1321 cggcaacaac ttcgcccttg cgcagtctgg cagtggtaac agtgccaacc gcaccgattt
     1381 taaaccgcaa aaagccaaca ccagcgagat tggcaccaaa tggcaggttc tggataaacg
     1441 cctgttgctc accgccgcgc tgttccgtac tgatatcgaa aatgaagttg agcaaaatga
     1501 tgacgggact tactcgcaat acggtaagaa acgcgtcgaa ggctatgaga tatccgtggc
     1561 agggaatatc actcccgcgt ggcaggtgat tggcggctat acccagcaaa aagcaaccat
     1621 caaaaacggc aaagatgttg cccaggatgg ttcctcatcg ctgccgtata ccccggagca
     1681 cgccttcacc ttatggagcc aatatcaggc aaccgatgat atctctgttg gcgcgggcgc
     1741 acgctatatc ggcagtatac ataaaggttc agacggcgcg gtgggaacgc cagcatttac
     1801 agaaggttac tgggtcgccg atgccaaact ggggtatcgg gttaatcgca atctcgactt
     1861 ccagctaaac gtttacaacc tgtttgatac cgattacgtc gcctcaatca acaagagcgg
     1921 ctaccgttat cacccgggcg agccaagaac cttcttgctc acagccaata tgcatttctg
     1981 attcagatgt ggggcgcagg ccccactttt tggagaactt gtatgatgta ccacattccc
     2041 ggcgtgttat cgccacagga cgtcgcccat tttcgcgaac aactggaaca agccgaatgg
     2101 gtggatggac gcgtcaccac cggagcacaa ggtgcacaag ttaagaacaa tcagcaagta
     2161 gacacccgca gcgcgttata cgccgcactg caaaatgagg tgctgaacgc ggttaaccaa
     2221 catgcattgt tctttgccgc tgccttgccg cgtacccttt ccacgccgct gtttaatcgc
     2281 tatcagaaca atgaaaccta tggtttccat gtggatggcg cagtacgcag ccatccacaa
     2341 aacggctgga tgcgcactga cctttctgcc acgctgtttt taagcgatcc acaaagctac
     2401 gacggcggcg aactggtcgt taatgacacc tttggacaac atcgggtaaa actcccggca
     2461 ggtgatctgg tgttgtatcc ctccagcagc ctgcattgcg tgacacccgt aacccgcggc
     2521 gtacgagtgg catcatttat gtggatccag tcgatgatcc gcgatgataa aaagcgcgcc
     2581 atgctgtttg aactggacaa aaatattcag tcgctgaaaa gccgctatgg tgaaaatgaa
     2641 gagatcctgt cgctgctcaa tctttatcat aatctgctgc gggaatggtc ggagatctga
     2701 cgcttgaact gcccaggttc gtccctatat tagctacact taactgtaca agcattgata
     2761 tggggaggac gatatggcat ccgcttgggc taacgatgac gccgtcaacg aacagatcaa
     2821 cagtacaatt gaagatgcga ttgcccgcgc tcggggtgaa attccgcgtg gcgaaagcct
     2881 gtatgaatgt gaagagtgcg gtgcccccat cccgcaagcc cgtcgggaag ccattcctgg
     2941 cgtgcgctta tgtattcatt gtcagcagga gaaagattta caaaaaccag cgtatacagg
     3001 atataatcgc agaggttcga aagacagcca gttacgttaa ctacaactgg cgagatgcat
     3061 agcgagtata atttgtattt tgcgtggcat tccgtgcctt taacgctgct gtgactgcca
     3121 cgcggcataa aacaagtcca taagtacaac aaataaatgg tttatcagta tgttagatat
     3181 caatcaattt atttgaacaa ggcggtcaat tctcttcgat tttatctctc gtaaaaaaac
     3241 gtgatactca tcacatcgac gaaacaacgt cacttataca aaaatcacct gcgagagatt
     3301 aattatgaaa actatcaata ctgttgttgc tgctatggct ctttcaactc tgtcatttgg
     3361 cgtgttcgcg gcggaaccgg taacggcatc ccaggcacag aacatgaata aaatcggcgt
     3421 ggtttctgcc gatggcgcat ccaccctcga tgccctggaa gcga
//
LOCUS       DADLSE010000259         3460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLSE010000259 DADLSE010000000
VERSION     DADLSE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.261"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..987)
                     /locus_tag="JN692_004121"
     CDS             complement(<1..987)
                     /locus_tag="JN692_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7266240.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLL"
     gene            complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN692_004122"
     CDS             complement(1044..2405)
                     /gene="allB"
                     /locus_tag="JN692_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HAY7266241.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHICHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(2465..>3460)
                     /locus_tag="JN692_004123"
     CDS             complement(2465..>3460)
                     /locus_tag="JN692_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoin permease"
                     /protein_id="HAY7266242.1"
                     /translation="LSLPGLITFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGG
                     MAIWAISLVGLGPIFDYIPSGIQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAR
                     SFREQALGQTLGLVVAYILFAVAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFA
                     VLVILMTTISTNATGNIIPAGYQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQD
                     SIYLFLDIIGGMLGPVIGVMMAHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSV
                     TLVAVILSLGGKFIPFMEPLSRVSWFVGVIVAFVAYALLKKRTTAEKTGEQKTIG"
BASE COUNT          982 a          927 c          803 g          748 t
ORIGIN      
        1 cagcaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc cagtctgtgt
       61 taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg gtacggttat
      121 cagcgtcata aatccggtcg ccacaaagct acgacgataa cgcgtattcc ccgcgccctg
      181 ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga tattcaccag
      241 acctgtaatc accgccgtca gaataattcc cggcgacaaa gcaccgccac tgccgagcgg
      301 gaaccactgc cagtgcaact caccggagag cgaatgcgaa gaaggaaagc aaaagtacca
      361 caacaaccag ccggttatcg taccgaccag caggccataa cgggcaaaac gttgcggcag
      421 aaagataatc atcgccagta ccaggcacat caccgccacc gagagcgcga acggtggtaa
      481 ctgaatttta aaatttgggt cggctatgcc aaacggcagt ccgagcatac ctttgaaaaa
      541 gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg acggcgtaaa
      601 taaccgtgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca gcacaccgga
      661 gagtgcaatc cctaccgcca gactggtagc gatatcgttg atcggtgtcc cgcgcgatgc
      721 ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacctggcc cttccataat
      781 agcgcgacga tgaccgcaaa acgcctgagc gaagcaggcc agtgcggtag caagaaaagc
      841 atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa gtagcgtagg
      901 aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg ataacaggct
      961 ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg cccgccgcaa
     1021 ggacgggcag tgcaggggcc agattactgc tgatgtttaa ggataaattg acctttcggc
     1081 gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat ggttttcgtg
     1141 atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata ttccagatcg
     1201 tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc atcttttcct
     1261 ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt cgccattaat
     1321 ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa catcacgtcc
     1381 atacagtttt gcagaccagc aataccgccc catgcttcca tgatgttgcc ggctttcatt
     1441 tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt aaacagtttt
     1501 tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt cgccagagta
     1561 ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga ttcacaggta
     1621 acatcctgac cttcctgacg tgcacgagtc acttcttcaa caccttccgg gctgctgata
     1681 tggcaaatgt gcagacggca accggcaact ttcgccaggt acagtacgcg gcgaatcgct
     1741 tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac gcgaccttca
     1801 cgtttcgctt cttcgcccag tgcatcacag atcagcgcgt tttcgcagtg caccagcacc
     1861 ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc gtttacatca
     1921 cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt gaagccgaca
     1981 acgcccactt catccaactc atgcagacga tcaatgttgt aagacaccag gccaccgagt
     2041 tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc aattgaagca
     2101 cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt gataccacct
     2161 tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc cggttcagaa
     2221 atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc atccataact
     2281 tcttttgcat cgcccagatc ctgaccgata gcagcaattt ttccgccttt aacggcgata
     2341 tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat taaatcaaaa
     2401 gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc aatatcggga
     2461 ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt ttctttaata
     2521 aggcgtaggc cacaaaggcg acgatgacgc cgacaaacca tgaaacacgc gataacggtt
     2581 ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga gttactgaaa
     2641 acgcagtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg gtatacagtt
     2701 catcaagatt aattttccca cgcatcacca caaaataatg cgccatcatg acaccaatta
     2761 ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc tgattttcca
     2821 ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg ccgtttttat
     2881 aggtcagttt cgtcggtgca atagcggcaa tctgataacc ggcaggaata atattaccgg
     2941 tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac gaggcgaaca
     3001 agctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg taatgaatac
     3061 tggctccggc aataatacat acccccgcga ccgcaaacag aatataggcc acaactaaac
     3121 ccagcgtttg ccccagcgct tgctcacgaa acgagcgggc gttttgcgta aagtcggatg
     3181 cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt tatcaccacc aggaacagga
     3241 agccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt ggaccgagcc
     3301 cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg cacgggttaa
     3361 gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca acgttgacca
     3421 gccagaagag taagaaagta attaagcccg gtagagaaag
//
LOCUS       DADLSE010000260         3319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLSE010000260 DADLSE010000000
VERSION     DADLSE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.262"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..858)
                     /locus_tag="JN692_004124"
     CDS             complement(<1..858)
                     /locus_tag="JN692_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7266243.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGQVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQCFFRDHNI
                     TIEMTESDAE"
     gene            complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JN692_004125"
     CDS             complement(877..2754)
                     /gene="bglF"
                     /locus_tag="JN692_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY7266244.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2888..>3319)
                     /gene="bglG"
                     /locus_tag="JN692_004126"
     CDS             complement(2888..>3319)
                     /gene="bglG"
                     /locus_tag="JN692_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7266245.1"
                     /translation="EALTIIDKRLGVQLPKDEVGFIAMHLVSAQMSGNMEDVAGVTQL
                     MREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASINDSDESLQQAVKQNY
                     PQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
BASE COUNT          871 a          897 c          752 g          799 t
ORIGIN      
        1 ttctgcgtca ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacactgcat
       61 atagccggga tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc
      121 ttccatggcc tgcaacatat cctgtggctg gcaggtgagg gggtaaacct gcccaccgag
      181 aagcatattg ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc
      241 actggcaacc agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc
      301 cagccccacg cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca
      361 taacgctact ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc
      421 gatgaccgcc cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta
      481 atgggataac gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc
      541 gtaaaacgct aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc
      601 ccaggcaatg gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc
      661 cgggtaacgg tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg
      721 cggttccatt tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc
      781 atcttccagc caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt
      841 ttctggaaat gctttcataa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg
      901 ttcacctgcg cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt
      961 actgattaat accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa
     1021 agaaatcagc cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa
     1081 tttgccgtcc agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc
     1141 tgactcaatg ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg
     1201 agaacgcact tcaccaaccg agggcagaat ggcgatacct ttacccaaca ggccactggc
     1261 aaacgtggta tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat
     1321 accgccctgc tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc
     1381 tggctgacgt ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat
     1441 ggcaatgaca ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat
     1501 ggtctgcatg aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta
     1561 gccaatgatg gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg
     1621 cggcaggttg acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc
     1681 tgatcccgcc accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc
     1741 cccgacctgc gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac
     1801 ggtgaagtta ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat
     1861 ttgccagaag ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag
     1921 ataaccggcg gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt
     1981 gacgggtgtg ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg
     2041 taaccacgca ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg
     2101 aataaccgat gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg
     2161 cgcatccgct ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc
     2221 accaatcacc atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag
     2281 gattatcggg aagaaccaaa acaaggcatc actggcgctg aataaaatta aataagtacc
     2341 actttgttcg gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc
     2401 ggttgccgcc atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg
     2461 gtttagcaga ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc
     2521 tgccacgctg ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa
     2581 ctgaccaccg ctttccacca ccataataat accgggggtc tttttcagta cctccgcttg
     2641 cgctttgctt tcatccttta acttaaaacg taatcgcgtt gcgcaatgca tcagactcac
     2701 aatgttatct gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg
     2761 ccctctaccg ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga
     2821 atcaagcaat ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa
     2881 taatatttca gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg
     2941 cgggtgaaat tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt
     3001 gccatgcttg cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa
     3061 ttgaagcatg ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat
     3121 aactcaagct ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt
     3181 cgcgcattaa ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga
     3241 ccagatgcat ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat
     3301 caataatggt taacgcttc
//
LOCUS       DADLSE010000261         3303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLSE010000261 DADLSE010000000
VERSION     DADLSE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.263"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(211..372)
                     /locus_tag="JN692_004127"
     CDS             complement(211..372)
                     /locus_tag="JN692_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266246.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(369..572)
                     /locus_tag="JN692_004128"
     CDS             complement(369..572)
                     /locus_tag="JN692_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7266247.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYPLMQAIVAEGDVVVSGATEPE"
     gene            817..1152
                     /gene="iraM"
                     /locus_tag="JN692_004129"
     CDS             817..1152
                     /gene="iraM"
                     /locus_tag="JN692_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7266248.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1433..1564)
                     /locus_tag="JN692_004130"
     CDS             complement(1433..1564)
                     /locus_tag="JN692_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7266249.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2064..2140)
                     /locus_tag="JN692_004131"
     tRNA            complement(2064..2140)
                     /locus_tag="JN692_004131"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2104..2106),aa:Arg,seq:tct)
     gene            complement(2241..2316)
                     /locus_tag="JN692_004132"
     tRNA            complement(2241..2316)
                     /locus_tag="JN692_004132"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2281..2283),aa:Ile,seq:cat)
     gene            complement(2460..3281)
                     /locus_tag="JN692_004133"
     CDS             complement(2460..3281)
                     /locus_tag="JN692_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7266250.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          951 a          880 c          606 g          866 t
ORIGIN      
        1 ccgttcccac cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca
       61 taccccgtct cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg
      121 atggctctga taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta
      181 acgatacctt ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag
      241 caccagactt tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag
      301 tgcatttagt cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg
      361 acgtagtatc actccggctc agttgcacca ctgaccacca catcaccttc tgctacaatc
      421 gcctgcatca ggggataagg ggttatggcc accggactac caaacggctg ccagccctct
      481 ttcagtttat gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca
      541 tagtgtttaa atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga
      601 catgtaatac agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga
      661 tatgcatgga taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa
      721 caacatcaga aaaataattc tgctctatgg tttaattcaa aaatatcatt tattcttttc
      781 agaacatcac cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca
      841 gttatccagc catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata
      901 atctggtatc aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca
      961 ggcatcatgc tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat
     1021 aaaacattct ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta
     1081 ttgaatgaat gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa
     1141 caaagtccat aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag
     1201 aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc
     1261 agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca
     1321 cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca
     1381 accgcctgaa ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt
     1441 ctggcgtttt ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac
     1501 cgtcagccgg cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa
     1561 tcacttttta ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg
     1621 aagttcatcc ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa
     1681 ttctgaatta gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat
     1741 aataacgtct cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa
     1801 ggcctttgct cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga
     1861 cgcctcatca atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa
     1921 aaccattaga ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca
     1981 aacatataat attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg
     2041 gaaggaatct ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg
     2101 cttagaaggc aattgccctg tccggctgag ctaacaacgc tgaataccga taatggaccg
     2161 ccatcgggga cccgcccccg caccaacaac cctgttatcg tgtcgtctgc tcttcctgat
     2221 aagctaatgg cggtttgtga tggtggccct tgctggattt gaaccagcga cctggcgatt
     2281 atgagtcgct cgctctcacc actgagctaa agggccggga gcagaataat aacggtccgt
     2341 aattaatttc gcaataaaaa acccgctcag cggcgggttg tagaaactct tctaacgtca
     2401 ggcataaaac gcccatcatt atgacgaatt taccacagat tccggaaaaa tcaaccttgt
     2461 tacctagtta ccttttttaa ctgccgctca gcccatgctt cttcaatatc aaaccgggtc
     2521 accagcgcat cataaaattt cttaactgtt ttttcccatg acgcgcgtgt tatctggttt
     2581 gtcacctcgc atatagcatt aaatgcctcc gttgatggta gtctttcata gccacgacca
     2641 ccacaacgct ggcagtctct gataacaggc ataccacgtt ttaccgactc ttcacgatga
     2701 atggcgacac cacgcccacg gcaatcctta caggcggtgg aaacctcacc ctttccgcca
     2761 cactccggac aggcaacttt taccacctcc ctgacttttt tccattcttc ccagtaagac
     2821 ggatacacac ctttcgtaca ctttgcccat accggcggct taccatccgg atactggatc
     2881 ttatttgtaa aaactatgct ttcaataaat ttttccccat agcaacaagg gcactgcttt
     2941 ttactcgctg cgctgcgggc ataatcctca aaagcgtacg aagccataat gcgcatcact
     3001 accggtttta tttctgccgg aagttttctc aacgccgcca cacgatcgca ccgactgagt
     3061 gcataatctg ccagtaattc tgttgcccgc gccctgtcat tcatactgat gcccattttc
     3121 cccaggaacg cagaaaaacc catctcagcc cgattctgtg tcatgccctg cgcggccatc
     3181 acatcagtga tactcagcgc atcttttgac gttgaggccg atgcatcggt caggccaggg
     3241 gattttgggg agtagtattt cggtaaatct tccagtttca ttttttgacc tgctcttaat
     3301 gca
//
LOCUS       DADLSE010000262         3281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLSE010000262 DADLSE010000000
VERSION     DADLSE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.264"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            143..391
                     /locus_tag="JN692_004134"
     CDS             143..391
                     /locus_tag="JN692_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266251.1"
                     /translation="MNNPIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            724..2247
                     /locus_tag="JN692_004135"
     CDS             724..2247
                     /locus_tag="JN692_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7266252.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2379..2598
                     /locus_tag="JN692_004136"
                     /pseudo
     CDS             2379..2598
                     /locus_tag="JN692_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2998..3207
                     /locus_tag="JN692_004137"
     CDS             2998..3207
                     /locus_tag="JN692_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266253.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT          910 a          642 c          700 g         1029 t
ORIGIN      
        1 gtgttgcgat acacagctga tgatatgtaa cttctaatta ttaagtataa gtttatagaa
       61 aactcattca tcttattttt gtctgtcgct ttagactata cacagcataa ttttattggg
      121 ttaatatttc tacgaggctg acatgaataa tccaatccca gagagattta tttttcaatg
      181 tgctttgttt aaaaatctcg agagggaggt gttcatgacg catgggtatg ttgatagtca
      241 tattattgat caggctttac gcttgcgttt aaaagatgaa accagtgtga ttctttctga
      301 tctctatctg caaatattgc agtacattga aatgcataag actacactaa cggatatcat
      361 tattaatgac agggaatccg tgctctctta gaacttgagt aaggttttag aataaacatc
      421 agctgtatca ccatgctgat gcaaagtgag catttcaggc gttatgcttt cttattatgt
      481 ccgcaatatc aggtgtcaag aatggagagt tctcgctctc cattcttgac acctgatatc
      541 ccgcctaact tattttgtac tgcctacatt atgctcaata attaatgggt tagatcaaga
      601 tggagaaaat tttatcttcg gcgtggatat ttcttatgct ctctaccatg catctctgta
      661 gatgccagcg cgagcgaatg aatggcattt tcatgtatta aaccctttgg ccgggacaag
      721 caaatgcgca atacactcat acccatcctc gttgcgatat gcttgtttat taccggtgtc
      781 gctattttga acatccagct ctggtattcc gcaaaagcag agtacctggc gggagcaaga
      841 tatgccgcca acaatatcaa tcatatactt gaagaagcgt cacaggcgac tcaaacagca
      901 gttaacattg ccgggaagga atgcgacctc gaggagcaat atcagcttgg cactgaagca
      961 gctctgaaac ctcacctgcg cacaatcatc attctcaaac agggaatagt ctggtgtaca
     1021 tccctgcctg ggaatcgggt cctgctgtct cgtattcctg ttgtcccgga cagtaattta
     1081 ctgttggctc cggcaatcga caccgttaat agattaccta tcctgctcta tcagagccaa
     1141 tttgcagata cgcgcatttt ggttacgata agtgatcaac atattcgcgg ggcgcttaat
     1201 gtacccttga aaggggtaag gtatgtatta cgcgtggcgg atgacattat tggaccaacg
     1261 ggtgatgtga tgacgcttaa tggacattat ccctataccg agaaggttca ctccacaaaa
     1321 tatcatttca ctattatctt taacccgccg ccactcttta gcttctacag acttatcgat
     1381 aaaggctttg ggattttaat atttattctg ttaatcgcct gcgccgctgc cttcctgctt
     1441 gatagatatt tcaataaaag cgcaacgcct gaagagatcc tgcgacgggc aataaataat
     1501 ggggagatcg tcccttttta ccaacctgtg gtaaatggtc gggaagggac attgcgggga
     1561 gttgaggtgt tagcccgctg gaaacaacct cacggtggat atatttcacc cgcggcattt
     1621 attccacttg ctgaaaaatc cggattaatc gttccgctta cacaaagcct gatgaatcag
     1681 gttgccagac agatgaacgc tatcgcgagt aaactgccgg aaggttttca tattggaatt
     1741 aattttagcg cttcgcatat tatttcgccg acgtttgtcg acgagtgttt aaatttccgt
     1801 gacagtttta cccgccgcga tttaaacctt gttctggaag tcaccgagcg tgagccattg
     1861 aatgttgatg aaagtctggt tcagcggttg aacattctgc atgaaaatgg ttttgtcatc
     1921 gcgctggatg atttcggtac tggctactca gggctttctt atcttcatga cctgcatatt
     1981 gattatatca aaattgatca cagtttcgtt ggccgcgtaa acgcagaccc agaatcaacc
     2041 cgaattctgg attgtgtatt ggatctggcg cgtaaacttt cgatcagtat cgtcgctgaa
     2101 ggtgtcgaaa cgaaagaaca acttggctat ctgaaccaaa ataatatcac atttcagcag
     2161 ggttattatt tctataaacc tgttacatac atcgacctgg tcaaaattat cctttctaaa
     2221 ccgaaggtga aggttgtggt tgagtgaaaa ttgatcagta aggccatagt gcggtgtaat
     2281 tatagacagc taattagctc gttgcctctt gttactattg ttcattattt tgtttgctat
     2341 aattgtttga aagttttgac aggattgcca ttagtagcat gaacaatagt aataatctgg
     2401 attgtttcac tctctatatc atcttttcca ttgcatttat gctgatcacc ctcctggtca
     2461 tccttattgc aaaacccagt accgggctgg gagaagtgct tgtgacgata aatttgctta
     2521 atgcccttgt ttggctggcg atcaatctgg ttaatcgatt taagagaaag actcgtcagc
     2581 cacagggatc agcaataatc tttcagtttc tcactgtcag tatgcggctg aatgggttgc
     2641 tggcagtgaa cgcctggatc attgaaggaa aggaattatt gcgcaaatag ttgtcaacct
     2701 tggtgttatc acagttgttt ttatatatca ccgaaataat taccatggca actattaata
     2761 attttgatgt cgaagagtta tttgttaaac aaaatagtca ccccaaagtg atcaatgtca
     2821 tgaaaataag gtgaaaaatg ataatgccga cttatttatc atttatatat tgtcgctgtt
     2881 tatctattta aattttttgt gcttttgttt ttgtctttac cctccgtttt ggtaatctga
     2941 gctcataggt tatttgacca ggaatgtttg ttaatccaat gaatggatgg ttaatctatg
     3001 aaactgaaaa aactccccgg gtttagtttg ggacttattg ctctggcggt gggtaatgca
     3061 tatgctgaac agtttattca gcacgatgaa tataaacatg ctgtggcaat aaaagatgga
     3121 aaagtaaaag atattgataa tgcgctagtt ataacaacgg gtaatggaag ttacgggtat
     3181 ccgttgcagg agagggtagc aagttaacca ttaacaatgg cgtcattgta acaacgggcg
     3241 gtttatatcc ttctgacaat agtcctctaa gcccagcatt a
//
LOCUS       DADLSE010000263         3264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLSE010000263 DADLSE010000000
VERSION     DADLSE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.265"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..263)
                     /locus_tag="JN692_004138"
     CDS             complement(<1..263)
                     /locus_tag="JN692_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266254.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSV"
     gene            288..527
                     /gene="relB"
                     /locus_tag="JN692_004139"
     CDS             288..527
                     /gene="relB"
                     /locus_tag="JN692_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HAY7266255.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            527..814
                     /gene="relE"
                     /locus_tag="JN692_004140"
     CDS             527..814
                     /gene="relE"
                     /locus_tag="JN692_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7266256.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            886..1041
                     /gene="hokD"
                     /locus_tag="JN692_004141"
     CDS             886..1041
                     /gene="hokD"
                     /locus_tag="JN692_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7266257.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1259..1510
                     /locus_tag="JN692_004142"
     CDS             1259..1510
                     /locus_tag="JN692_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266258.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVASLQALKEIGIKAGFSAFSEE"
     gene            1577..1855
                     /locus_tag="JN692_004143"
     CDS             1577..1855
                     /locus_tag="JN692_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266259.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1857..2906
                     /locus_tag="JN692_004144"
     CDS             1857..2906
                     /locus_tag="JN692_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7266260.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLLFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            2919..>3264
                     /locus_tag="JN692_004145"
     CDS             2919..>3264
                     /locus_tag="JN692_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7266261.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYP"
BASE COUNT          831 a          772 c          871 g          790 t
ORIGIN      
        1 aacactgaca tgctaaagca aaaaatagat gaataagttg agttgtgcat atgtagcctg
       61 accgtcacaa agtatatggt gtctgtacca gtaagatgat ggccggactc tttaaaaaag
      121 agctgacctg cacaatacag gatggactta gcaatggctg ctcctggcac aaagcggaca
      181 gtgatcaccg ttcttacgac tactttctga cttccttcgt gacttgccct aagcatgttg
      241 tagtgcgata cttgtaatga catttgtaat tacaagaggt gtaagacatg ggtagcatta
      301 acctgcgtat tgacgatgaa cttaaagcgc gttcttacgc cgcgcttgaa aaaatgggtg
      361 taactccttc tgaagcgctt cgtctcatgc tcgagtatat cgctgacaat gaacgcttgc
      421 cgttcaaaca gacactcctg agtgatgaag atgctgaact tgtggagata gtgaaagaac
      481 ggcttcgtaa tcctaagcca gtacgtgtga cgctggatga actctgatgg cgtattttct
      541 ggattttgac gagcgggcac taaaggaatg gcgaaagctg ggctcgacgg tacgtgaaca
      601 gttgaaaaag aagctggttg aagtacttga gtcaccccgg attgaagcaa acaagctccg
      661 tggtatgcct gattgttaca agattaagct ccggtcttca ggctatcgcc ttgtatacca
      721 ggttatagac gagaaagttg tcgttttcgt gatttctgtt gggaaaagag aacgctcgga
      781 agtatatagc gaggcggtca aacgcattct ctgaaccaaa gcatgacatc tctgtttcgc
      841 accgaaggtg acacttctgc tttgcgttga caggagaagc aggctatgaa gcagcaaaag
      901 gcgatgttaa tcgccctgat cgtcatctgt ttaaccgtca tagtgacggc actggtaacg
      961 aggaaagacc tctgcgaggt acgaatccga accggccaga cggaggtcgc tgtcttcaca
     1021 gcttacgaac ctgaggagta agagacccgg cgagtgagaa atccctcgcc acctctgatg
     1081 tggcaggcat cctcaacgca cccgcactta acccgcttcg gcgggttttt gtttttattt
     1141 tcaacgcatt tgaagttctg gatggtgccg gaatagaatc aaaaatactt aagtagcgcg
     1201 cagggataaa agggatggac cccgaacagg ggagtgctac ttatctggaa ggattctgat
     1261 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
     1321 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
     1381 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
     1441 tgtcgcttca ttacaggcat tgaaagagat cggaataaaa gctggctttt ctgctttttc
     1501 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
     1561 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aatcatgata attcgaccgt
     1621 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1681 ctcatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga
     1741 ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca ggctccctta
     1801 ctccgaacag ccaccgcgtc cgtcccgcac attccagcaa cggatctgga ggtaacgtgc
     1861 gggtattact tcgacctgtt ctggttccgg aactcgggct ggtggtcctt aagcccggtc
     1921 gtgaatccat gccggtattc cacaataccc ggttactggt ggagccggaa ccgaaaagca
     1981 tgcgtaatct gccgtccggg gtcgttcctg ccgctcgcca gccgctggtg gaagacaaaa
     2041 cattgctgct gtttttcagt aacgcacggg tgattcgtgc tgctggtggt gctggtgcat
     2101 tgtctgactg gctgttgcgc catattaaat cctgccagtg gccacacggc gattatcatc
     2161 acagcgaaac cgtcattcac cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg
     2221 acaaccagct gcgtgaccag acatccgaat cactcgagca acttgctcat caaaacctgt
     2281 cagcatggat gattgacgtc atcggtcacg caataagcgg tacgcaggag cgtgaattat
     2341 ctctagctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2401 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2461 gcgacatcgt accgggagag cagaccgcca ccagcatact gaaacagcgc acaaaaaatc
     2521 ttgcgccgct gcctcacgcc caccagcaac agaacccacc acaggaaaag acggtggtca
     2581 gcattgccgt tgatcctgag tctccggaat ctttcatgaa acgacctaaa cgtcgccgct
     2641 gggttaacga gaaatacaaa cgctgggtga agacacagcc gtgtgcgtgt tgtggtaagc
     2701 cagccgacga tccccatcac ctgattggtc atggtcaggg cggaatgggg acaaaatctc
     2761 acgatatttt cacgctaccg ctgtgtcggg agcatcacaa cgagcttcat gcggatcctc
     2821 tggcgttcga agaaaagcat ggttctcagg ttgatttaat ttttcgtttt cttgatcacg
     2881 cctttgcaac tggcgtgctt gggtaaaaga ggtgactgat gctcatagat ttggttttac
     2941 cttacccgcc gacggtgaac acttactggc gacgccgtgg cagcacatat tttatctcgg
     3001 aggagggaaa gcgttatcgc cgggctgtgg cgcttattgt tcgccagcag cggctgaaat
     3061 taagcctgtc cggaaggctg gcgataaagg tgattgcaga gccaccggat aagcgtcgtc
     3121 gcgacctgga caatatcctg aaagcaccgc tggatgcgct gacgcatgcg ggagtgttaa
     3181 tggacgatga gcagtttgat gaaatcaata tcgttcgtgg tcagccagta tctggtggac
     3241 gtctgggggt gaagatttac ccca
//
LOCUS       DADLSE010000264         3238 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLSE010000264 DADLSE010000000
VERSION     DADLSE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.266"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            21..611
                     /locus_tag="JN692_004146"
     CDS             21..611
                     /locus_tag="JN692_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7266262.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            851..1111
                     /locus_tag="JN692_004147"
     CDS             851..1111
                     /locus_tag="JN692_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7266263.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1335..1409
                     /locus_tag="JN692_004148"
     CDS             1335..1409
                     /locus_tag="JN692_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7266264.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1402..2259
                     /gene="repA"
                     /locus_tag="JN692_004149"
     CDS             1402..2259
                     /gene="repA"
                     /locus_tag="JN692_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7266265.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2959..3099
                     /locus_tag="JN692_004150"
     CDS             2959..3099
                     /locus_tag="JN692_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266266.1"
                     /translation="MAENGYGLAGLGVGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CR"
BASE COUNT          839 a          811 c          831 g          757 t
ORIGIN      
        1 ttgccaggag cagataaata atgaagttga ttatttttat tctcattgtt ctgattattg
       61 cagcgctact tatccggatt atcctcagaa gtgtaaatca gcattctcct ttgctgatgc
      121 aattgcatgc ggcaggtata cggaccggtg atgcagagcg aatactgtcc agcggtgaat
      181 actggcaacg tcagaagacg ctgctgacag aaagggaagt cagttttatg aaaggactgt
      241 tcagaattgt ggatatgaag cggtggtatc tgtgtccgca ggtacgggtc gcggatatcg
      301 tccagctgaa cgggaatatc cggccacgat cacgccagtg gtggcagtta ttcaggatgg
      361 tgtctcagtg gcatgttgat gtggttatcg ttgagctgcg ttcgttcagt attgttgcag
      421 cagtagagct ggatgatgcc agccatttac gaccggaacg cagacgccgg gatattcttc
      481 tggaagaggt tctgaggcag gctggtattc cgttgctcag aagccacgat gccagaaaac
      541 tgctgcagat gaccggagaa tggctgaata caacaggggc tgatcagcag tcaccggaac
      601 atcgtagctg acgccttcgc gttgctcagt tgtccaaccc cggaaacggg aaaaagcaag
      661 ttttcctcgc tcccggcgtt tccataactg aaaaccatac tatttcacag tttaaatcac
      721 attaaacgac agtaatcccc gttgatttgt gcgccaacac agatcttcgc cacaattctc
      781 aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg
      841 aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcattgagtc aaaagcggtt
      901 tgtgcgcaga ggtaagccta tgactgactc tgagaaacaa atggccgctg ttgcaagaaa
      961 acgtcttaca cacaaagaga taaaagtttt tgtcaaaaat cctctgaaag atctcatggt
     1021 tgagtactgc gagagagagg ggataacaca ggctcagttc gttgagaaaa tcatcaaaga
     1081 tgaactgcag agactggata tactaaagta aagactttac tttgtggcgt agcatgctag
     1141 attactgatc gtttaaggaa ttttatggct ggccacgcca taaggtggca gggaactggt
     1201 tctgatgtgg atgtacagga gccagaaaag caaaaacccc gataatcttc tttaactttg
     1261 gcgagtcaga aagattaccg gggcccactt aaaccgtata gccaacaatc aagctatgcg
     1321 gggagtatag ttatatgccc ggaaaagttc aagacttctt tctgtgctcg ctccttctgc
     1381 gcattgtaag tgcaggatgg tgtgactgat cttcaacaaa cttattaccg ccaggtaaag
     1441 aacccgaatc cggtgttcac accccgtgaa ggtgcccgga cgcttccgtt ctgcgggaaa
     1501 ctgatggaaa aggcggtggg cttcacctcc cgttttgatt tcgccattca tgtggcacat
     1561 gcccgttcgc ttggtctgcg tcggcgcatg ccaccggtac tacgtcgccg ggccattgat
     1621 gcgctgctgc aggggctgtg ttttcactat gacccactgg ccaaccgcgt ccagtgttcc
     1681 atcaccacgc tggccattga gtgcggactg gcgacggagt ctgctgccgg aaaactctcc
     1741 atcacccggg ccacccgggc cctgacgttc cttgcagagc tgggactgat tacctaccag
     1801 acggaatatg acccgcttat cgggtgctac attccgaccg atatcacgtt cacatctgca
     1861 ctgtttgctg ccctcgatgt atcagaggag gcagtggccg ccgcgcgccg cagtcgtgtg
     1921 gaatgggaaa acagacagcg caaaaagcag gggctggata ccctgggtat ggatgaactg
     1981 atggcgaaag cctggcgttt tgttcgtgag cgtttccgca gttaccagac agagcttaag
     2041 tcccgtggaa tgaagcgtgc ccgtgcgcgt cgtgatgcgg acaggcagcg tcaggatatt
     2101 gtcaccctgg tgaaacggca actgacgcgt gaaatctcgg aagggcgctt cactgccagt
     2161 cgtgaggcgg taaaacgtga agtggagcgt cgtgtgaagg agcgcatgat tctgtcacgt
     2221 aaccgtaatt acagccggct ggccacagct tccccctgaa agtgacctcc tcagaataat
     2281 ccggcacgcg ccggaggcat tcgcacgcct gaagcccgcc agtgcacaaa aaatcagcgc
     2341 tacatacaaa aaacaacctc atcatccacc ttctggtgca tccggtcccc cctgtttttg
     2401 atacaaaaca cgcctcacag acggggaatt ttgcttatcc acattaaact gcaagggact
     2461 tcaccataag gttacaaccg ttcacgtcat aaagcgccag ccgtcagccc tacagggtgc
     2521 gatgtatctt ttaaacgcct gtttatatct cctttaaact acttaattac attcatttaa
     2581 aaaaaaaacc tgttcactgc ctgtcctgtg gacagacaga tatgcacctc ccaccgcaag
     2641 cggcgggccc caaccgaagc tggtttagtt acaacatgca aacataaacc cctaaaaaac
     2701 ccaagtccga cgtagaactg aaaccacaac gcccctcctc ataactaaaa agcgccgccg
     2761 cccccgcccg aagggcggga acagagtcgc ttttaattat gaatgttgta actacatcat
     2821 catcgctgtc agtcttctgg ctggacgtat cgagtacacg ctcgtaagcg gccctcacgg
     2881 cccgctaacg cggagatacg ccccgacttc gggtaaaccc tagtcgggac cactccgacc
     2941 gcgcacagaa gctctgtcat ggctgaaaac gggtatggct tggcagggct gggggtgggt
     3001 aaggtgaaat ctgtcaatca gtaccggctg acgccgggct tcggcggttt tactccggta
     3061 tcacatgtaa ccacagcgtg ccgccttcca tgccgctgac gcggcatcag aataatacaa
     3121 gcagcattta atgcatttgc aaaagtgtga gtaataaaga cttgttaagt ggtcgtttca
     3181 gggatatcgt taagacttga cgtataggaa attgaaaaac taagcgatac ccctgtcc
//
LOCUS       DADLSE010000265         3218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLSE010000265 DADLSE010000000
VERSION     DADLSE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.267"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            80..343
                     /locus_tag="JN692_004151"
                     /pseudo
     CDS             80..343
                     /locus_tag="JN692_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            970..1515
                     /gene="cobU"
                     /locus_tag="JN692_004152"
     CDS             970..1515
                     /gene="cobU"
                     /locus_tag="JN692_004152"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7266267.1"
                     /translation="MMILVTGGARSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDDGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            1512..2255
                     /gene="cobS"
                     /locus_tag="JN692_004153"
     CDS             1512..2255
                     /gene="cobS"
                     /locus_tag="JN692_004153"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HAY7266268.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2267..>3218
                     /gene="cobT"
                     /locus_tag="JN692_004154"
     CDS             2267..>3218
                     /gene="cobT"
                     /locus_tag="JN692_004154"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7266269.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQVGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLG"
BASE COUNT          758 a          683 c          903 g          874 t
ORIGIN      
        1 acaactagtg atagttgact tattttcgct cttgagagac aaccatactc aaatctccca
       61 cattgcagga gatttgaata tgaaaacgtc accgtggaat aaagaccgta ccatcggtca
      121 aaaaaggcca cttcagatat ctcatatctg ggggatccgg attagacttg agttggaagg
      181 taaaacacgc gatctagctc tgttcaacat ggctttggac agtaagctac ggggctgtga
      241 tttggtcaaa ctcaaagtat cagatgctgc atatggcagt tctgtctcaa gcagagcaac
      301 ggtgttgcaa cagaaaaccg gcagccctgt ccaatttgag atgcctttaa taacacatct
      361 tttaaatgtt gaagaattat cccgcctgct gaagaacgtt gctctgagtg tgataaacct
      421 ttttattctt gatgatgtca gcgagaagat ggaatatatc cccgactggg tcgtggttac
      481 ccggttaaac atctggtggc gctaataaat ctggcaagtc acattctgtt gaatgctgtg
      541 acagtgcctt accgagagag cgaaattgtg gtgttctagt gcatagtcgc caccattccg
      601 gataattcta ttgcgttgtt ttacaagttc ttttacagcg cagatctgtc gctgacgttg
      661 aatcaatagc ctggatttca gaacacaatg cccgtttgct ggttggtccg gcagtagcat
      721 taattcatta tctgaatgaa atgtgtcaat ccacagaaac cgctgtcaat gacgcatttt
      781 gtcggacgtt aacacgagaa agtctggcta ttccgtaatg ccatcttatt ttgtaatgga
      841 ttgagaatgc tggattttta attatattat gcgcagtatt aatcgcgtaa atgttttgcg
      901 gtcagcaacg gtgggggatc cattgctctg ttcaatgaat accatcatac atttgaatca
      961 ggagtcggta tgatgatttt ggtgacggga ggcgcacgga gcgggaagag tcgccacgca
     1021 gaggtgctta ttggggactc ttcacaggtt ctgtatatcg ctacctcgca aatccttgat
     1081 gatgagatgg ctgcacggat agaacatcat cggcaaagcc gcccggagca ctggcgcaca
     1141 gtggagcgct ggcaacatct tgatgaatta attcatgcag acattaaccc gaatgaggct
     1201 gtgttgctgg aatgcgttac cacaatggtg actaatctgt tgtttgatga tggcggcgat
     1261 aaagaccctg atgaatggga ttatcaggcg atggaacagg cgattaatgc tgagattcag
     1321 tcgttgattg ctgcctgcca acgttgcccc gcaaaggttg tattagtgac taacgaagtg
     1381 ggaatgggga ttgtgccgga gagtcgtctg gcacgacatt ttcgtgatat tgccgggcgg
     1441 gtaaatcagc agttggccgc tgcggcaaat gaagtatggc tggtggtttc gggtattgga
     1501 gtaaaaatca aatgagcaaa ttattttggg cgatgctctc atttattacg cgtctgcctg
     1561 taccgcgtcg ctggtcccag ggactggatt tcgagcatta ttctcgcggt attattactt
     1621 ttcctttgat tggattattg cttggcgcga ttagcgggct ggtcttcatg gtgctgcagg
     1681 catggtgtgg cgcaccactg gcggcactgt ttagcgtact tgtgctggcg ctgatgaccg
     1741 gggaattcca tctggacggc ctcgctgata cctgtgatgg cgtattttct gcacgtagcc
     1801 gcgatcgcat gttggaaatc atgcgtgata gccgtttagg cacccacggc ggtctggcat
     1861 tgatttttgt ggtactggca aagattctgg tgttaagcga gttggctctg cgtggcgaac
     1921 cgatccttgc atcgctggcg gcagcatgtg cggtcagccg cgggactgcc gcattattga
     1981 tgtatcgtca tcgttatgcg cgggaagaag ggcttggcaa tgtgttcatt ggcaaaattg
     2041 atgggcgaca aacctgcgtc acactcggct tagccgctat ttttgctgcc gtattattgc
     2101 cgggtatgca tggtgtggct gctatggtgg tgacgatggt ggcgattttc atcctcggtc
     2161 agcttctcaa acgtacgctg ggcgggcaaa cgggcgatac gctgggagca gccatagagc
     2221 ttggtgaact ggtcttcctg ctggcactgc tataaagaga cgtgacatgc aaataattgc
     2281 cgatttactg aatacgatcc cagccatcaa ttctgccgct atgtcgcgtg cacaacggca
     2341 tgttgacggg ttactcaaac ctgttggtag cctaggaaag ctggaggcgc ttgccataca
     2401 actggcagga atgccggggg tgaatggcat accgcatgtg ggcaaaaaag cggtactggt
     2461 tatgtgtgcc gatcacggcg tctgggagga aggggtcgct atttctccaa aagaagtgac
     2521 agccatacag gctgaaaata tgacccgtgg aacaaccggc gtgtgtgtgc tggcagcaca
     2581 agtgggcgct aacgtccacg taattgatgt tggtattgat actgctgagc ctatccccgg
     2641 gcttatcaac atgcgtgtcg cacgaggtag cggcaatatt gcttcagctc cggcaatgag
     2701 tcgccgtcag gctgaaaagt tgcttttgga cgtcatatgt tatacgcggg agctggcaaa
     2761 aaacggtgtc acgctgtttg gtgtaggtga actggggatg gcaaacacga caccggcagc
     2821 ggcaatagtc agcacaatca ctggccggga tcctgaagaa gtggttggga ttggtgcaaa
     2881 cctgccgaca gataaactgg ctaataaaat tgatgttgtg cgtcgggcga ttacgttgaa
     2941 tcaaccaaat cctcaggatg gtgttgatgt cctggcaaaa gtgggtggat ttgatttggt
     3001 cggaatagct ggagtgatgt taggtgctgc ttcctgcggt ttacccgtgt tgctggatgg
     3061 atttctttct tatgctgctg cgctcgcagc ctgccagatg tctcctgcaa tcaaaccgta
     3121 tctcattcct tctcacttgt cggcagaaaa aggcgcgcgt atagcgctct cgcatttggg
     3181 gctggagcct tatctcaata tggatatgcg tttaggtg
//
LOCUS       DADLSE010000266         3218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLSE010000266 DADLSE010000000
VERSION     DADLSE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.268"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1166
                     /locus_tag="JN692_004155"
     CDS             <1..1166
                     /locus_tag="JN692_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7266270.1"
                     /translation="IGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILR
                     AASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCY
                     IIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQR
                     GKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQAC
                     ECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEH
                     PFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVEL
                     VIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(1169..2026)
                     /locus_tag="JN692_004156"
     CDS             complement(1169..2026)
                     /locus_tag="JN692_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7266271.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2039..2773)
                     /locus_tag="JN692_004157"
     CDS             complement(2039..2773)
                     /locus_tag="JN692_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7266272.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(2788..>3218)
                     /locus_tag="JN692_004158"
     CDS             complement(2788..>3218)
                     /locus_tag="JN692_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7266273.1"
                     /translation="KDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVH
                     GIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHH
                     RVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT          781 a          837 c          839 g          761 t
ORIGIN      
        1 acatcggcgg cgataaaagc attccttatc tgaatattcc ccaggaagag aacccgttcc
       61 tcggctatcg cgcggtacgt atttacccgg aatttgctgg cctgttccgc actcaactgc
      121 gggccatttt gcgcgccgcc agtttcggca acgcccagtt gatgatcccg atggttcaca
      181 gcctcgatca gatcttatgg gtgaaaggcg agatccaaaa agcgatcgtt gagcttaagc
      241 gcgatggcct gcgtcatgca gagacgatta cgcttgggat catggtggaa gtgccgtcgg
      301 tgtgctacat catcgaccac ttctgcgatg aggtcgattt cttcagtatc ggctccaacg
      361 atatgaccca gtatctgtat gcggtcgatc gtaataaccc gcgcgtatcg ccgctatata
      421 acccgattac gccatcgttc ctgcgcatgt tgcagcaaat agttaccact gcgcatcagc
      481 ggggcaaatg ggtaggcatt tgcggtgaac tgggcggtga aagccgttat ctgccgctac
      541 tgcttgggct gggcctggat gagctgagta tgagtagccc gcgtattccg gcggtgaaaa
      601 gccagcttcg tcaactggat agcgaggcgt gtcgggaact ggcgcgtcag gcatgtgaat
      661 gccgcagtgc gcaggaaatt gaagcgctac tcaccgcctt tacgccggaa gaagacgttc
      721 gcccactgct ggcgctggag aatatctttg ttgatcagga ttttagcaat aaagagcagg
      781 cgatccagtt cctgtgcggc aacctcggcg ttaacgggcg cactgaacat ccgtttgagc
      841 tggaagaaga tgtctggcag cgggaagaga ttgttaccac cggcgttggt tttggcgtag
      901 cgatcccgca caccaaatct cagtggatcc gtcattccag tatcagcatt gcccggctgg
      961 cgaaaccggt tgactggcag tcagaaatgg gcgaagtcga actggtgatc atgctgacgc
     1021 tgggtgctaa cgaagggatg aatcatgtga aagtcttctc gcagctggcg cgtaaactgg
     1081 tgaataaaaa cttccgccag tcgttgtttg ccgcgcaaga tgcacaaagt atcctgacgc
     1141 tgctggaaac agaattaacc ttctgacgtt agccctgaaa acgggcgctg tactctcccg
     1201 gcgtcagacc aaactgacgc cggaaaacgc gacaaaaata gtcgctatcc ggaaaaccgc
     1261 aacgctgtgc cacttcatga attgacagat gatatttctg caaaatcatc ctcgccttcg
     1321 ccattcgcac ccaacgcaca tactcgataa aacgcatcgt tccatgctga gcaaacagtt
     1381 ttgacagatg attgggcgtg atattaaaaa actgcgccac gctctcgcgg gtgagcggct
     1441 gggcataatt atcctgaacc cagttacaaa tgctgtgata gagaaaatcg ccgcgcggtt
     1501 gcgaattacc cggcggcata ttcaccacgt tgcggcaaag atgcagcaaa cttaacacca
     1561 gtggctgaat gatattctgc tccagcggcg aacggctaag atgggtgagg gcggtaagca
     1621 tcgcttcacc ttcaccgcgt tgcaggctgg gaagctggat ctggcgagca ggatgcaaca
     1681 acgacgtggt gcggctatcg taaaacgata acccaagcca ggtcggtgcg aacaccaggc
     1741 ttaacagcat caccggtttg ttgttgatcg gtaaattagc cgcgcgtgcc ggaataaaca
     1801 gcatttctcc ttccaccatt cgctgctgct ttgcttccag caggttgccg tattcgccgc
     1861 gcagcacgat atccagacgg ggaaaatcaa tacataaact gcccaccggt aacgaggcag
     1921 gctggctggc aaaccagacg cgccccagta gttgcgggtt aagcaccagg ccgctgaaca
     1981 gatcggcgaa aaattgttga tcggcgggca agcctggtgc tttcatgaaa gttcccgatt
     2041 aaagatgcat taactggcga aattctttca ctttgctgcg gctgaccggc acttcaaaat
     2101 ccagatcttt cagtcgcaga atgtaggtgt tattaaacca cggttcgatt tcgcgtattt
     2161 tgttcagatt gacacaaaat gagcgatggc agcggaaaaa atgcgacggc ggcagtttgc
     2221 tgcaaaattc ggtaatgttc atcggcatta cgtaggattc acgccgcgta tagacaaagg
     2281 tcattttctc gtgcgcttcg gcgtaataga tatcgttaat tggcgtgacg attattcgct
     2341 catctttcac cagattaatg gtgtcattct cacgcgttac cgtcgcggca ggcgtactgc
     2401 tggtctgctg ttgttgccag gccgcttcca gtttttgcag catcccggta atacgtgact
     2461 cctgatacgg tttgagaatg tagtcaaacg cctccagttc aaacgcttct accgcatgtt
     2521 ctttccacgc ggtgatgaac acaataaacg gtttatgggc gaactggctg atgttttgcg
     2581 ccagcaacac gccatccagc gacggaatat tgatatccag aaaaatggcg tcgacgcggt
     2641 tatgctgcaa aaacttcaac acgtccagac cgtcgtcaaa ggtgccgaca atctccatct
     2701 ggctgtgctc tttaattagc cagctcagtt cctgttgtgc caggaattcg tcttcaacaa
     2761 tgatgacttt cacaatatca ctccggctca aagcaataac gtagcctgtg aggcgactgg
     2821 ggtgcgttgg ttaggaatgt aaaacgcaat ttccgtcccc ggctccaggc ggcggatatg
     2881 cagcccctcg ccatacaata acttcacgcg atgatggaca ttcagcaggc caattttatt
     2941 gcccggcatt tcattcgctt cgacccgctc aatcaccttt ggatcgatgc cgtgcccggt
     3001 atctcgcacc gcaatgcgta cccgatttcc gcactctgca acgctgatgg tgacaacgcc
     3061 tttacctttg caaggctgaa taccgtggac aatggcgttc tccaccaacg gctggatcag
     3121 caggctggga atgcagcaat tcacctcttc atcaatatca tagataaccg tcagcttgtc
     3181 gccaaagcgg gcctgctcaa tggcaatata gtctttaa
//
LOCUS       DADLSE010000267         3124 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLSE010000267 DADLSE010000000
VERSION     DADLSE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.269"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            52..771
                     /locus_tag="JN692_004159"
     CDS             52..771
                     /locus_tag="JN692_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7266274.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCIYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            782..2209
                     /locus_tag="JN692_004160"
     CDS             782..2209
                     /locus_tag="JN692_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7266275.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2202..2897
                     /locus_tag="JN692_004161"
     CDS             2202..2897
                     /locus_tag="JN692_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7266276.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          799 a          675 c          890 g          760 t
ORIGIN      
        1 tgggggggaa acatcgggca taaatgggca tgaagtaatg gagtattagt tgtgaatgtc
       61 aatttctttg tcacctgtat tggtgacgcc ctgaaatcaa gaatggcacg agactccgtg
      121 ctgctactgg aaaaactcgg ctgtcgcgta aatttcccgg agaaacaggg atgctgcggt
      181 cagcctgcga tcaatagcgg ttatatcaaa gaagcgattc cagggatgaa aaatctgatc
      241 gccgcactgg aggataacga cgatcccatt atttcaccgg ctggctcttg catctatgcc
      301 gtaaaaagtt acccgacgta tctggcggat gaacctgaat gggcatcacg tgccgaaaag
      361 gttgccgtgc gtatgcagga tctcacctct tttattgtta ataaattagg ggtagtcgat
      421 gtaggtgcca gtttgcaagg gagagctgtg tatcacccat cttgtagcct ggcccgtaag
      481 ctgggagtga aggacgagcc acttacgctg ctgaaaaatg tgcgtggact ggagctgttg
      541 acctttgctg aacaggatac ctgctgcgga tttggcggca cgttctcggt caaaatggcc
      601 gaaatatccg gcgagatggt gaaagaaaag gttgtgcacc tgatggaagt ccgccctgag
      661 tatttaattg gtgctgacgt gagttgcctg ctgaacatca gtgggcgatt acaacgggaa
      721 gggcagaaag tcaaagtgat gcatattgct gaagtgttga tgagccgctg aggatataaa
      781 gatgtcgatc aaaaccagta atacagattt taagacacgc atccgtcagc aaattgaaga
      841 tccgatcatg cgcaaagcgg tggcaaacgc gcagcagcgt attggggcaa atcggcaaaa
      901 aatggtcgat gaattggggc actgggagga gtggcgcgat cgggccgccc agatacgtga
      961 tcatgttctg agtaatctcg acgcttatct gtaccagctc tcagaaaaag tgacgcaaaa
     1021 cggcggtcac gtctattttg caaaaaccaa agaagacgct acccgctaca ttttacaggt
     1081 tgcccaacgc aaaaatgccc ggaaggtggt gaaatctaaa tcgatggtga ccgaagagat
     1141 tggtgtcaat catgtgttgc aggatgctgg cattcaggtg attgaaaccg atctgggtga
     1201 atatattctc cagctggatc aagatccgcc atctcatgtt gtggtcccgg caattcataa
     1261 agatcgccat cagatccgtc gagtgctaca cgaacgtctg ggctatgagg ggccggaaac
     1321 gcctgaagcg atgaccttat tcatccggca aaaaatccgt gaagatttcc tcagtgctga
     1381 aataggtatt accggctgta atttcgcggt ggcagagacc ggttcggtat gcctggtgac
     1441 caatgaaggt aatgcgcgaa tgtgtaccac gctgcctaaa acgcatattg cagtgatggg
     1501 aatggagcgt attgccccca cgtttgccga ggtagatgta ttgatcacca tgctggcgcg
     1561 cagtgccgtt ggtgcacgtt tgacgggata caacacctgg ctgacagggc cgcgcgaagc
     1621 ggggcacgtt gatggtcctg aagagtttca tctggttatt gtcgataacg ggcgttctga
     1681 ggtgctggcc tctgaatttc gggatgtgct gcgctgtatt cgctgcgggg cttgtatgaa
     1741 tacttgtccg gcatatcgcc atattggcgg tcatggatat ggctctattt atccagggcc
     1801 aattggtgcg gtgatttctc cgctacttgg cggctataaa gattttaaag atttacccta
     1861 cgcctgctct ttatgcactg cttgtgacag cgtgtgtccg gtgcgtattc cgctgtcaaa
     1921 actgattttg cgtcatcgtc gggtgatggc tgaaaaaggg atcaccgcaa aagcagagca
     1981 acgggcgata aaaatgttcg cttatgccaa tagtcatcca ggattgtgga aagtcgggat
     2041 gatggccggc gctcatgcgg caagctggtt tatcaatggc ggcaaaacac cactcaaatt
     2101 tggcgcgatt agcgactgga tggaagcacg cgatcttcct gaagctgacg gagagagttt
     2161 ccgtagttgg tttaagaaac atcaggcgca ggagaaaaag aatggataat cggagcgaat
     2221 ttttgaataa cgttgctcag gcactgggtc gcccgctgcg acttgaaccg caagcagaag
     2281 atgcgccgct taacaactat gctaacgagc ggcttaccca acttaaccaa cagcagcgct
     2341 gtgacgcgtt tattcagttt gccagcgatg ttatgttgac gcgctgtgag ctgaccagcg
     2401 aggcgaaggc ggcagaagct gcaatacgtc tgtgtaaaga gctgggagat cagtcggtcg
     2461 tgattagcgg tgacacgagg ctggaggaat tggggattag tgaacgtttg cagcaggaat
     2521 gcaatgccgt tgtttgggat ccggcgaaag gtgccgagaa tatctcgcag gcagagcagg
     2581 ctaaagtggg tgtcgtgtat gctgaatatg gtttaaccga atcgggaggc gtggttcttt
     2641 tttccgccgc cgagcgcggg cgttcattga gcctgctccc ggaatcttct ctttttatcc
     2701 tgcgtaaaag cacaatcctg ccgcgtgtag cgcaactcgc agaaaaattg catcagaaag
     2761 cgcaggccgg tgaacgaatg ccgtcatgta ttaacatcat cagtggtccc agctcaacgg
     2821 cggatattga gcttatcaaa gtcgtcgggg ttcatggtcc ggtgaaagcg gtgtatctga
     2881 ttattgagga ttgctgagag agatatgaga tctgaacata aacctgaaga aggcatacga
     2941 ggttgaaaat agcgttaagg acaacctgac ttaaccccgt tccgtcatgg ttatgctgct
     3001 cttctgtaaa tatcctggca ttagtaatgc caggatattt acagtgaaaa agacaggcat
     3061 ccattcctga aaacgggttc ccttcccggt cattgtcact tcagctttat acaggcactc
     3121 tcta
//
LOCUS       DADLSE010000268         3113 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLSE010000268 DADLSE010000000
VERSION     DADLSE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.270"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..345
                     /locus_tag="JN692_004162"
     CDS             <1..345
                     /locus_tag="JN692_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7266277.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
     gene            335..1153
                     /locus_tag="JN692_004163"
     CDS             335..1153
                     /locus_tag="JN692_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7266278.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGANPRRRQWSITTDTGISYAT"
     gene            1143..2567
                     /gene="patD"
                     /locus_tag="JN692_004164"
                     /pseudo
     CDS             1143..2567
                     /gene="patD"
                     /locus_tag="JN692_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            2777..3113
                     /locus_tag="JN692_004165"
                     /pseudo
     CDS             2777..3113
                     /locus_tag="JN692_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
BASE COUNT          729 a          688 c          868 g          828 t
ORIGIN      
        1 atcctgcccg ttcaggcggc gcttgagcgt ttgccgccgt cattgttgca ggcgtcggct
       61 gatctcggcg cacgtccacg acaaaccttt cgctatgtgg tgctgccgct ggcaatcccg
      121 ggtattgccg ctggctctat ctttaccttc tcactcacac tgggcgattt tatcgtcccg
      181 cagctggttg gtcctccagg atattttatc ggcaatatgg tttattccca gcagggggcg
      241 attggcaata tgccgatggc ggcggcattc accctggtgc cgattattct catcgcactg
      301 tacctggcgt tcgtgaaacg tctgggagcg ttcgatgcac tctgaacgcg ctccgttttt
      361 cctcaaactg gcggcctggg gcggcgttgt tttcctacat tttcccatcc tgataatcgc
      421 cgcctatgcg tttaacactg aagatgcggc gtttagtttt ccaccgcagg gcctgacgct
      481 gcgctggttt agcgtggcag cacagcgtag tgatattctt gatgccgtga cgctgtcact
      541 taaagtggcg gcgctggcga cattaattgc gctggtgtta gggacgctgg cagctgccgc
      601 gctgtggaga cgagactttt tcggcaaaaa cgccatttcg ctgttattgc tgctgcccat
      661 tgcgctgccg ggcattgtca ctggtctggc gctattaacc gcctttaaaa ccatcaatct
      721 ggagccggga tttttcacca tcgtggtcgg tcatgcgact ttttgcgtag tggtggtgtt
      781 taacaatgtc atcgcccgtt ttcgccgcac ctcgtggagt ctggttgagg cgtcaatgga
      841 tcttggggcc aatggctggc aaaccttccg ctacgtagtg ttgccgaatc tcagttcggc
      901 gttactggca ggaggaatgc tggcgtttgc cttgtcgttc gatgaaatca tcgttacgac
      961 ctttacggca ggtcatgaac gaacgttacc gttgtggttg ctcaatcagc ttgggcgacc
     1021 gcgtgatgta ccggtaacca acgtggtggc actgctggtt atgttggtaa caaccttgcc
     1081 gatcctgggg gctaacccgc gaaggcgaca atggtcaata accactgata caggaataag
     1141 ctatgcaaca taagttactg attaactgag aactggttag cggcgaaggg gaaaaacagc
     1201 ctgtctataa tccggcaacg ggggacgttt tactggagat tgccgaggca tccgcagagc
     1261 aggtcgatgc tgctgtgcgt gcggcagatg cagcatttgc tgaatggggg caaaccacac
     1321 cgaaagcgcg tgcggaatgc ctgctgaaac tggcggatgt tatcgaagaa aatggtcagg
     1381 tttttgccga actggaatcc cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg
     1441 aaatcccggc gattgtcgat gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg
     1501 gtctggcggc aggtgaatat cttgaaggtc atacttcgat gatccgtcgc gatccgttgg
     1561 gggtcgtggc ttctatcgca ccgtggaatt atccgctgat gatggccgcg tggaaacttg
     1621 ctccggcgct ggcggcaggg aactgcgtag tgcttaaacc atcagaaatt accccgctga
     1681 ccgcgttgaa gttggcagag ctggcgaaag atatcttccc ggcaggcgtg attaacatac
     1741 tgtttggcag aggcaaaacg gtgggtgatc cgctgaccgg tcatcccaaa gtgcggatgg
     1801 tgtcgctgac gggctctatc gccaccggcg agcacatcat cagccatacc gcgtcgtcca
     1861 ttaagcgtac tcatatggaa cttggtggca aagcgccagt gattgttttt gatgatgcgg
     1921 atattgaagc agtggtcgaa ggtgtacgta catttggcta ttacaatgct ggacaggatt
     1981 gtactgcggc ttgtcggatc tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac
     2041 tgggtgctgc ggtggcaacg ttaaaatctg gtgcgccgga tgacgagtct acggagcttg
     2101 gacctttaag ctcgctggcg catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag
     2161 cgacagggca catcaaagtg atcactggcg gtgaaaagcg caagggtaat ggctattact
     2221 atgcgccgac gctgctggct ggcgcattac aggacgatgc catcgtgcaa aaagaggtat
     2281 ttggtccagt agtgagtgtt acgcccttcg acaacgaaga acaggtggtg aactgggcga
     2341 atgacagcca gtacggactt gcatcttcgg tatggacgaa agatgtgggc agggcgcatc
     2401 gcgtcagcgc acggctgcaa tatggttgta cctgggtcaa tacccatttc atgctggtaa
     2461 gtgaaatgcc gcacggtggg cagaaacttt ctggttacgg caaggatatg tcactttatg
     2521 ggctggagga ttacaccgtc gtccgccacg tcatggttaa acattaagtt agatgttagt
     2581 ggctgttata ggacggccac tatcgtttta ggttctgagt ttaatggcgg ttaagtatta
     2641 gaatgattac attaataact agcgtaaaaa ttaggctaaa tgtttgatgg cgatttttca
     2701 tcctgtcaca gttgattagc ataaggcttt agagtatcgt tattaaacta ataatttata
     2761 agtagaagta ttaacaatga aagttcaatt gtacaaattc acggaagata aaaataaaac
     2821 ccttaccttc agatggacga aaaaacactt tgagttttgt atggataata aaatattttt
     2881 aaatcataag gggaagaagt cgtataaaga aaggaattta tttcttttct caaaaggtga
     2941 caagataaca attgaaaata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa
     3001 tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat
     3061 aggtcgattt tgtagtattg aataatgggt ggaaatcatc ctttgaatcg ttt
//
LOCUS       DADLSE010000269         3097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLSE010000269 DADLSE010000000
VERSION     DADLSE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.271"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            25..801
                     /locus_tag="JN692_004166"
     CDS             25..801
                     /locus_tag="JN692_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HAY7266279.1"
                     /translation="MSAQSVNEIISKIGADAVALPENFRIHDLEKFNLNRFRFRGVLS
                     TASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAP
                     FSALLSVNGERHSQKSLAEWIEDWADYLVGFDANGDAIQATKAAAAVRKITIEANQTA
                     DFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVL
                     VLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            929..1453
                     /gene="yfdR"
                     /locus_tag="JN692_004167"
     CDS             929..1453
                     /gene="yfdR"
                     /locus_tag="JN692_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7266280.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPTPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPTVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFKELTES"
     gene            1562..2429
                     /locus_tag="JN692_004168"
                     /pseudo
     CDS             1562..2429
                     /locus_tag="JN692_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2470..2769
                     /locus_tag="JN692_004169"
     CDS             2470..2769
                     /locus_tag="JN692_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator protein PrtN"
                     /protein_id="HAY7266281.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKAKAKAKAN
                     ACKLPVPTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTGKQKGKEHH"
BASE COUNT          956 a          619 c          618 g          904 t
ORIGIN      
        1 gcaattaatc aaatccatgc tcttatttct gctcagagtg ttaatgaaat tatcagtaag
       61 attggtgccg atgctgtggc attgcctgag aatttccgca ttcatgatct ggaaaaattt
      121 aatttaaatc gcttccgttt ccgtggtgtg ctttccactg ccagcatcga tgactttacc
      181 cgttattcta aagatcttgc agatgaaggc acccgctgct ttatcgatgc cgataatatg
      241 cgagccgtca gtgtgcttaa cctgggtact attgatgaac caggtcacgc agataacacc
      301 gccactctca aactgaaaaa gacagcaccg ttctctgctc tgttgtctgt taatggcgag
      361 cgtcattccc agaagtcact ggcagaatgg attgaagact gggccgacta ccttgtgggc
      421 tttgatgcta atggtgacgc tattcaggca acaaaagcgg ctgcggctgt ccgtaaaatc
      481 acgattgaag caaaccagac cgctgatttt gaagataatg acttcagcgg caaacgctcc
      541 ctgatggagt ctgtcgaagc gaaaaccaaa gatattatgc cagtggcatt tgaatttaaa
      601 tgcgttccgt ttgaaggtct gaaagaacgt ccatttaaat tacgcctcag cattatcact
      661 ggcgatcgtc ctgtactggt tctgcgcatt attcagctgg aggcggtgca ggaagaaatg
      721 gctaacgaat ttcgtgatct gcttgttgag aaattcaaag acagcaaagt agaaaccttt
      781 attggtactt tcaccgcctg atttcattac tgcaaatgcc cctgcggggg catttatgga
      841 aacgtaattg actcaataat cgccggatgg tgagggcttc cttttaccag aattcagcgt
      901 ggtgcagcac atatacgcgg agaacaaaat gtcatttatt aaaacttttt ccgggaagca
      961 tttttattat gacaggataa ataaagacga catcgtgatt aacgatatcg cggtttccct
     1021 ttcaaatatc tgtcgctttg ccggtcatct ttcacacttc tacagtgtcg cccaacatgc
     1081 ggtgctttgc agccagctgg tgccgcagga atttgctttt gaagcgttaa tgcatgatgc
     1141 aacagaagcg tattgccagg acatccccac gccgctgaaa cgccttcttc ctgactataa
     1201 acggatggaa gaaaaaatag acgccgtaat ccgtgagaaa tacgggttac ccacggttat
     1261 gagcacgcct gtgaaatatg ccgatctcat catgctggca accgaacgcc gcgatctcgg
     1321 gcttgatgat ggctctttct ggcctgtact ggaaggtatc ccggcaacag agatgttcaa
     1381 agttattcca ctgtcgccag gccatgccta tgggatgttt atggaacgct ttaaagagtt
     1441 aactgagtcg taaaaatcag cacgtacgaa ttcaaactct gccataaaag aacatataag
     1501 taatttatta acatatagat ataggttata ttacaaattg aaaaattatt ggagagcaac
     1561 aatgaatcaa aatccattct cattctatga ctttcttgga tatttaatac caggtggttt
     1621 ttttatccta ttaatgtatt tctgtggttt gacattcgat ctagatattg ttattgactt
     1681 aactgaattg ctcagaggtc aaagtcaaat ctttggcatt ttgaactatg gttcaatagt
     1741 aatcatatct tacatagctg gacactttat atctatcaca tccgcatttt ttattgaaaa
     1801 atatatgaac gaagaattgc aatatccgtc agaatatcta tttaataagt tatcagatga
     1861 caaaaaaaaa cgtcttaaca ccatcaagtg acaaacagaa cgaagacaca aagagaaaaa
     1921 taaaatactg cataatcaaa gtaatgttat tcccgataat accatgggat tacgccactc
     1981 aaaaactatg ttattcacaa tcattgccat ttcaattagc taataccaca tggctaatga
     2041 tcaaagaagg ttatgaaaaa aaattcagaa tagacgataa attattaaac atcaggacag
     2101 gtcttcatga tgatcttttc agattggctt atcattacgt ctatgaattt tctaatcaac
     2161 atcaaacaaa aattcaaaac tatgttgcat tatatggttt ttgcagaaat atttgtttag
     2221 tatttgtaat tacattttgg atatcatttc taacattaat ttatcgtgct atagaaggtg
     2281 gggcggtgtc ttatatttct atttcgacgc tacttagctt tttcttcgtc tatgtttttt
     2341 atgttggatt cgttaagttt tatagacgat atactttaga ggtattaatg gcatttgctg
     2401 tacttcagag caaagatgct attaattaat atattaccct cgggtgcagc cgggataatg
     2461 gagaaattta tgaacacctt gtttttactg atggctgaat tcaatacctc aaacattgaa
     2521 ctctcagcag ttagccaaaa gtactttggt atgagtccag ccacagcaga agcaaaagca
     2581 aaagcaaaag caaaagcaaa cgcttgtaag ttgcccgttc caacatatcg catcggcaca
     2641 tcacaaaaag caaaacgttg catcaatatt caggatcttg cggaatacat agacaaaaga
     2701 cgagaagaag gacgtatcga gtgggaacag gtcagaacag gcaaacagaa gggcaaagaa
     2761 catcactaaa gaaaaaaccc gcctaaaggc gggttttcaa aaagcaccag ctatgatcat
     2821 gctgctttgc gacgacgaag cttaccctgc tgctctttac cagagacagt agcgtgagtg
     2881 aacgcattag gagcagcctt catcagaact tcaaagcagc ccccatacct gcgaatgctt
     2941 tcattgtgtc gaacttaagc tgtggcttgg ttgctttttg atcttccata gaaaactcca
     3001 gaagttatac cgaaacaatt cctgttgttt actcatcatc aatagatgat acgcaatatt
     3061 tatttttaaa tttaaggttc tttggcgtaa cttcatc
//
LOCUS       DADLSE010000270         3055 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLSE010000270 DADLSE010000000
VERSION     DADLSE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.272"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            368..2020
                     /locus_tag="JN692_004170"
     CDS             368..2020
                     /locus_tag="JN692_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7266282.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPKITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JN692_004171"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JN692_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JN692_004172"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JN692_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2512..3035)
                     /locus_tag="JN692_004173"
                     /pseudo
     CDS             complement(2512..3035)
                     /locus_tag="JN692_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
BASE COUNT          871 a          662 c          717 g          805 t
ORIGIN      
        1 atcggaaacg atgatctggc agtctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc aaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg gaaaacggaa ttttcttttt acacccccaa
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctattgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accggacgtg aacctctcag ctcatccggc
     2521 tcttctcatt gaggcctgtc cccatagtaa cggccagtgt gcaaacaacc tcgtttcact
     2581 ccgcgcaaca cgggccagcc agtggctggc ccgtcgtcgg tggcccctaa gtcgcttgtg
     2641 tttacggggc agccataaca cgagtttgcg atcgatttgc ctcagcgtca gatacagtgc
     2701 tgatttataa aaagcaccgt agtactttat ccagcctctg attttcgcat tgaacatatg
     2761 cgccagctcg tacaaggact tgtcactgcg attctcctca cctcctgacg gattgctttt
     2821 cctgctctgg cactcatcgc cggactgaag ttaacgaagg tcttacccca gcgattcatc
     2881 gacagacgcg gccggaatgt atagcccaga aagtcgaagc tcgtttccgg gtagtccccc
     2941 cgtcggtcag catccttaca gtaaactatt cgtgtttttt gcggatgcag ctgcagcccg
     3001 cattgcgcaa gccgctgcct cagtccactt atcagagcgt cagcctgcca ctggc
//
LOCUS       DADLSE010000271         2966 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLSE010000271 DADLSE010000000
VERSION     DADLSE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.273"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..298)
                     /locus_tag="JN692_004174"
     CDS             complement(<1..298)
                     /locus_tag="JN692_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266283.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEEL"
     gene            complement(298..1008)
                     /locus_tag="JN692_004175"
     CDS             complement(298..1008)
                     /locus_tag="JN692_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266284.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1005..1993)
                     /locus_tag="JN692_004176"
                     /pseudo
     CDS             complement(1005..1993)
                     /locus_tag="JN692_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          679 a          877 c          715 g          695 t
ORIGIN      
        1 tcagttcctc aggtaacagc cagtagagcc ggtaccggga tccgagcccc cgccagtcag
       61 ggtcactatt cccctccgtg tcggtaaaaa acaggtcccc ctgaaacggc aggtatccgt
      121 accggacaat ccggttatta ttcagacaca gcacgccata caggcacggt tcaccattaa
      181 caatgatatc gatatacatc cccgtagtac gctgattcag gcgaatggtg cagacctgag
      241 cgcccagtgt caccgtaaac tgctgggctt taacgggaga taaaacaatt tccagcatca
      301 ggtgatcccc ctgttcgtgg cgcttcgtct gtcagcgtct gacggttgtg tcaccgacgc
      361 cgtaactggc tgcgttttaa ccgatgcggc cccttttgct ttatcgttgt ccgtgggaga
      421 cgacttatcc gtacttccca ctgacacctc tcctgtattc attacggtct gaaataccgc
      481 agtaaccgtc agtaaggtcg gaccattatt acttcgcatt cggtagtcat atttcacaag
      541 gtcgcaggat gtccatgtct tgtccggcgt ctcaatatcg tagagtcctg ccgtggtacg
      601 catcctttca agcgtttcca gcacattcgt ccgcgaggtg gtggaaaaat ttgtcaggtt
      661 cgggacggat ccggaaaagg ccgtccaccc ctctacagtg aaagtcacat gcagttccgg
      721 cgatctctgg attttattaa aggtggtata agccccctgc tccacgggga cggtggaaac
      781 agaagcctcc gcgcccacct caacgacaac aaaagaatcc ggggagaaag gtttgccacc
      841 cttctgatgg gcacctgccg gatcattccg tgcgtaataa ataccgaatg acggtgccag
      901 tacactgtta atgagtccca ggacaccgcc gccacgaact gcactcagta cgttactttc
      961 actgagcgaa aagttattca gggaaatatt atcgaaagaa aaactcatcc tgttaccccg
     1021 ctggaataaa ccgacacaag cgccgaattc gtgatacgcc gacgtgcgtc atcggtaata
     1081 cccttcacat tgtccgacgt tgtggtgaca ttcagtgtcc cgatatgcgt ggtttccgtt
     1141 acggtggact gcgatacagg cgcaggatgg cgcgaccgta tggccattgc cgcacccgga
     1201 taaggcaggt tcgccagcac ccgcggaata tagttgcggg tctcctccgg agcagcagcc
     1261 agtcccttac gctgaacatt tccctctccc cagttatatg ccgccagagc cttagccaga
     1321 tcgccatgaa aaaaacgtat caggccacca agttttctcg cggcggcatc agcggctttt
     1381 tcgggatcaa aggcatcgtt ccccctcaga ccaaattcac tggccgtctt cggcatgaac
     1441 tgaaacagtc ccatcgcacc agcaggtgag acggcaaact gattaccacg ggattcggtg
     1501 atcgcaacgc tgcgcagcag tccgggcggc aggttatatt tttcctccag ttgagacagt
     1561 ttcggttgca gccagcctaa cagggcttcg tcggtcgctg gcggttttgc atggcattac
     1621 cgagtttttc ctgcgtcgca cgcataccct gtagccagga ttcaccgaag gctgcttccc
     1681 tcccggttgc ctgagaagcc tgagtatcca gcatgccctg ctgccatact gtgggtgatt
     1741 gtgcccgggt gatgttgcca gacttttctc cggcatccag ttttgtctga tacgtctcca
     1801 tctctttctt attgaaaaag aaagtcccgt ctgaagccca gaaaaaacca tgtgaatcca
     1861 gccagtcctt attttccctg ccaacaatgg atgtcagtaa tccgtcaaca accgggtaaa
     1921 gcgccgttat cgcaaaaaga aggcctccgg ggccactgag ggcttttgtc agcccccgca
     1981 cccatgacgc cactttcagc ccgatcagcg taataatgac attctgccag ccccccagtt
     2041 ctccggcagc cttattcgcc agggaggcca ctgactcaac gtcattcaga aatgtggtga
     2101 taaacctgtt cacttcctcc ggatgttgct gcatccagtc accgagtgtc tccagccagt
     2161 ggctgagctt cagcgcgtac ggcatcaacg cggtgctaat cgtcatcccg atgccggacc
     2221 aggtctgatc gagctcaaca agctcttttc gtagcttacg ggccagcgcc atacgccccg
     2281 gcgtaacctg cgaacgggaa gtaaattcgt ccacatcttt cagcgcatga ccggagccaa
     2341 gaaactgctg tccggcataa ctgaagccca gcgcgttgcc gtaggcagtt tgttcggact
     2401 ttgtcagtcg cggaaaaata accgccagct tgcgcatgat ggcttcagtg ctgtcagtat
     2461 ttaaatcaac actaatgccc gccctggctg caacctgaaa caaatcctgt aacacaggat
     2521 caaaggacct tccggctctg aaggccgctt ttgcatttgc aagccgggaa aatgccccag
     2581 acatttcccc ttcatcaaca ttatttgccc gcgcagcccg taaccatccg tccagatttc
     2641 tggccttcat gccaaaagca tcagattcca cggacagacg gttcagatca cgggcaaaac
     2701 ctgttaccag gttcttcagc ccccccagcg tcagactgac acccgcaaga gccagaatct
     2761 cgctacggat ccctgaaaaa aaacgggacg cccgtttccc ggcatcttcc attcgacggg
     2821 ccgttttatc agcatctttt ccggtttttt ccagtgattc actggttttc ttctgtccct
     2881 gactgaatgt gtcagcgaca ccttccatca ccgtcgtgag ccggttaagg gcagcatgta
     2941 ctgcctgttc cccggcagta aagtcc
//
LOCUS       DADLSE010000272         2964 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLSE010000272 DADLSE010000000
VERSION     DADLSE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.274"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(55..507)
                     /locus_tag="JN692_004177"
                     /pseudo
     CDS             complement(55..507)
                     /locus_tag="JN692_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(523..1419)
                     /locus_tag="JN692_004178"
                     /pseudo
     CDS             complement(523..1419)
                     /locus_tag="JN692_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            complement(1419..2024)
                     /locus_tag="JN692_004179"
     CDS             complement(1419..2024)
                     /locus_tag="JN692_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7266285.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(2195..>2964)
                     /locus_tag="JN692_004180"
     CDS             complement(2195..>2964)
                     /locus_tag="JN692_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707671.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7266286.1"
                     /translation="LQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPK
                     ADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPAVVL
                     KELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWW
                     STLAKSNLTFFKFGALSNRHTPPAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPL
                     LALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT          894 a          738 c          617 g          715 t
ORIGIN      
        1 ctaccgaaat atggtgctgg tttattagca gtaattttgt ggggatccct caggtgccag
       61 cggcacatac aatttaacct taaaggccgg agaagccagc accagcccac gaattgcagg
      121 tgcataatca tgaacccatg tggcaacaag cactgcgccg acgctttgcg cgatcactac
      181 cacctcttcc agtccgacct ggctgtcgct ggcagcaaaa cggacaaatt catcgacatc
      241 ctgcactgag cgcataagag acgggctata accacgcggc cccgaatttt ttccatgccc
      301 ccgggcatcc caggcataaa aagcagtatc aggcatcgcc agttcatcaa cgagatgttg
      361 tagacgacca gaatgttcat gcccgcgatg aaataagacg atgacctttt tcgcccctgg
      421 ctgtaaagtg ggccagtgcc gataatacaa cgctgtatta tcactggtgg taaaaaaatg
      481 ttccccaggg atgcgtgaat tttccatttt ctctattttc cttcagtaac agcagtagcg
      541 aataaaataa aaaaataccg gagcggtgaa aattaatgag tcaatcctgt caaggagtcc
      601 gccatgtcct agtaatagtt ttccactatc tttaacacca atatctcgtt tgatggctga
      661 catcactaca tcgccgcaaa aaccactaat accaattaac aaacccgcta ataatgcctg
      721 taatgtattt agcggtgtca gcaacggtcc aataatcagt gacgcaatca tggtggtgat
      781 tacgcccccc agcagacctt ccagcgtttt tcccgggctg actttgggga ccacttttct
      841 tctgccgcag gatttccccc ataaatactg tgcaatatcg tttgactcgg ttaacgccaa
      901 aagaaataac accagtaacg ccccgccctg gatatttatg gttggcaaca ttaatagcca
      961 ggcggcatga ctcaaggcaa aaacggtcat tatccatccc caaaaaatgg cgctcacggt
     1021 atgctgaaaa ccggaggggt cacccacaaa aactcgccag gtggctaata tcagaaaccc
     1081 agtcaaaggt ataaataaca gaaacagctc aaagcaatta aaaccgataa gcaaatagtt
     1141 aagaggaata acccaatata accaacgcga aaaatgtacg gatataagcg tacaatattc
     1201 tttcaatgcc agaaaactta tcagcgtaaa aaacgtcaac gtcatccagc gtgggcctga
     1261 aatcaccagt gaaaaacaaa tgacgatgcc ccactaggta cgaatacgta atgtaacttc
     1321 atcccctttt ctctcaggta accgccatag cagaaaacgg tttatcagcg ttgctaaaat
     1381 taacaacgcg aaaagtgttg ccagagattt ttccagcatt atctttcagc gctcataaga
     1441 agcacactcc gacaacgatt aatcgcagta cagataagca gaattgaagc aatgctccat
     1501 aaaagattat tccattgcat ccactgtgga taaatggcaa cggccagacc ccacagacca
     1561 aatattaacg cgcgatcact tttgccaaat ggaccggcat aactgcgaac accgttaatc
     1621 gtctgcgcga gtaaaccgca aaactcggtc aatatggtgc aaaataacat gagtatcacg
     1681 agtgatgcgt tactttccgg taaaaataaa aacggtaaat agagcgcaat atcggaaata
     1741 acatcgccag tctcattcaa aatcgccccc agacgtgttt gctgattgca ctcacgcgcc
     1801 aacatgccat ccagcgcatt gagcgccata cggataaaaa gcacgatggg caatagcaaa
     1861 aagaggatgg gttgtgccac caacaccaac agcaatccgg taaaaagaga aagcgccagt
     1921 gccgtaagag tgatgtgatt cgctgtaacg tggtgcttat aaagccaaaa catcgtcggc
     1981 cttaacagcg actgaaagag cggttttatc tggtagagcg tcattatcaa tccttgataa
     2041 ttcggtttgc gctatggtgc atcaattccc ctgacggaga tatcgcaaca cgccgcttca
     2101 ttaaaaattc agaatatatc aaggtattgg gggctcactg tcaggaatga ttatccccaa
     2161 taaatcaagt agttaaaaat tattacgctg ttattcaata caactcatca agtttcctca
     2221 tcgcttgttc ccgtattgcg cgcgtcttac cgttaagcgc cagctggcgg actaaatcca
     2281 gttcagggtt aacaagctct aacgccagca aaggatcacg tttcagcctc gccttcaata
     2341 catcaactgg aaagcgggga ttattcatcg ccacaatcca ccactcggga tcaatttctg
     2401 ccgccagtgc cgcagggggg gtgtggcggt tgctaagtgc gccgaacttg aaaaatgtga
     2461 gattgctttt cgccagtgta ctccaccata tacgatccgg ttgttttgcc agtacatcca
     2521 gttgtgctaa cgcatcgggg tcgagtgcat aataaacacg taatgcttca tcaacctgcg
     2581 cacgggagct attttcctgc ctcaggaaaa acttcgccca aatatcattg cgtggcgtgt
     2641 tttgctgtaa ttccttgaga acaacagcgg gtaaattatc ccaactaact aatgcactct
     2701 ctaaaaagtc ccagtcttcc aaacgtatca gggcggcggc atttaatagc atctggtcat
     2761 caacttcgcc gtggctaatc agctcagaca aaatcatatc agcgctggtt tcatatgcat
     2821 ctgaatctgc tttcggatta tttttccgat gcgataaagc acgttgccaa attttttgtt
     2881 tcatcgacgt gctcagatac tgatttttcg ccagattact ccacccctca ggatcgttga
     2941 aacgtgtcaa taaccgttgt aaag
//
LOCUS       DADLSE010000273         2955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLSE010000273 DADLSE010000000
VERSION     DADLSE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.275"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..578)
                     /locus_tag="JN692_004181"
     CDS             complement(<1..578)
                     /locus_tag="JN692_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7266287.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPSEQKLVSGFVEYLYESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAA"
     gene            complement(645..905)
                     /locus_tag="JN692_004182"
     CDS             complement(645..905)
                     /locus_tag="JN692_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266288.1"
                     /translation="MKIEELREIFSEDGLYTVCVENDAIVSHCCIKCLQFQQRKSGVA
                     LIYFADGLVTDGFLLRGDEFVTSLRALKETGLKAGFSAFECE"
     gene            complement(1129..1341)
                     /locus_tag="JN692_004183"
     CDS             complement(1129..1341)
                     /locus_tag="JN692_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7266289.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIATALVT
                     RKDLCEVRIRTGQTEVAVFVDYESRE"
     gene            complement(1557..2102)
                     /locus_tag="JN692_004184"
     CDS             complement(1557..2102)
                     /locus_tag="JN692_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7266290.1"
                     /translation="MNSVSVENLPSLTHQHLPVITTELLAELYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDELRELKNRNSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDSYFNQYEKERATGKMNIDIHLAIRDDKTAFIRSYAPGQLVTVEEALML
                     LQSRGWLVMPRDELAGKLMEL"
     gene            2636..2923
                     /locus_tag="JN692_004185"
     CDS             2636..2923
                     /locus_tag="JN692_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HAY7266291.1"
                     /translation="MAQVYFDTLKFVETLEAAGMPAAQARAISAAVKDSHEAAEVATK
                     RDLNDVRTELKNDIAALDAKIDKLSLQLTVRFGGMLVVAVGALTAIIKLPL"
BASE COUNT          734 a          765 c          674 g          782 t
ORIGIN      
        1 gcggccacat tttcgacttg ttccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gcacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc gtgtcgcaac ggtattcaga tattgctctg cggcatcact caaggctgga
      301 gtgtgttccc tacctactga ttcgtacagg tactcaacga agcctgaaac cagtttttgt
      361 tcgctaggcg tgatcgcccc tccgaccgga gtccagtaat cgaaccccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgaacgcgct taaaatcagc gtgtatccac
      481 tcgcctgttt tgatttgatg cagaaattcg caactctccg gcgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      601 gttactcagc aggggggcaa ccctgcactg aattgtagat gaactcactc acattcaaaa
      661 gcagaaaagc cagccttaag tccagtctct ttcagcgccc gtaatgacgt gacaaattca
      721 tctccacgca aaagaaaacc gtccgtcaca agtccgtccg cgaaataaat taacgctact
      781 ccactctttc tttgttgaaa ttgtaaacat ttaatgcagc agtgactgac aatagcgtca
      841 ttctcaacgc acacagtata gaggccatct tcactaaaaa tttcacgtaa ttcttcgatt
      901 ttcatcaaca gaatccttcc agataaatag ctctcccctg ttcggggtcc acccctcttc
      961 tccctgcgcg ctacttaagt gtttttgatt ctattcctgc gcattctgaa agttcaaacg
     1021 cgttgaaaat cgcaacaaaa aacccgccga agcgggttaa gtgcgggtgc gttgaggatg
     1081 cctgacacat cagaggtggc gagggatttc tccctcgcct ggtcactctt actctctaga
     1141 ttcgtagtct acgaagacag cgacctccgt ctggccggtt cggattcgta cctcacagag
     1201 gtctttcctc gttaccagtg ccgtcgctat gacggttaaa cagatgacga tcagggcgat
     1261 taacatcgcc ttttgctgct tcatagcctg cttctccttg cctttcggca cgtaagaggc
     1321 taacctacat gtgttcagca tggattgagc ctcagattaa tgttaagcgt cttgcaggac
     1381 gcgtaatgtt aactggggct tttctctatc tgccttttgg tgttcatgcc tgaggcagat
     1441 agcctcaggc acccgcagca attctacttg acttcccttt caccgcaaat cattttatcc
     1501 tcggtggtaa tgttctcccg atatgggaat tcccatatcg gggctaattt aaccagttaa
     1561 agctccatta attttccggc aagttcatct cgcggcatta ccagccatcc gcgcgattga
     1621 agcaacatca gggcttcttc aaccgtcacc agctgaccag gcgcataact tcggatgaag
     1681 gcagttttat catcacggat cgccaggtga atatcgatgt tcattttccc cgtagcccgc
     1741 tctttctcgt actggttgaa gtagctgtct tccagttttt cgaatacttc ccacgcctga
     1801 tcggtttcga gcattttggc gtgacgggct gcacctcgtt ctgtccagag gatgagtgag
     1861 cgaacattgc gggcgatttt cacagaaact cttaaagagt ttctgttctt cagttcccgt
     1921 aattcatcgc cttcgatcag gaaaaagtgt tttccctcta taaaccgctc tttgtttcta
     1981 ttgaaattgt ttgtcaaacg ctggcgctct gttccgtaca gctcagccag aagttctgtt
     2041 gttataacag gaagatgttg atgtgtaagt gatgggaggt tttcaacaga tacgctgttc
     2101 atgaatatgt cctactgaat attttgattt accccttttg agagggtgac cgggcgctca
     2161 aaaccgtcag tagtcggcgg gcgtattccc attgctggta ttgtattagc cgcacgccca
     2221 gtcataaacc aagaattctg gacacaaaaa aaaccgcatg tctgtcgggc gcggtaaccg
     2281 ctactgaaag gtgttttgag caccgtcgag gaatgtaatc tgctaacatg aaactgtcaa
     2341 tacattcatg ttccaatcat cgggtggcga actgtgcggg gttagcacta ccgggtacgg
     2401 aaaccggcga gcctttcggc tcccccacac agcccgccat aaatcgcgaa tgtgactatg
     2461 caaacgatat gaaaaaagac gcgggcgcgt ctcatatcgc tccgtaaaca tccggggtgc
     2521 taatcccgac gccagatttt gctggcgcgt gaggaatata gccccggatt aacgttgtcg
     2581 tcaacagcca ctacaggcta aactaacaat attccccaac aacgaaggat ttaacatggc
     2641 tcaggtctat tttgatacac tgaagttcgt cgaaaccctg gaagccgcag gaatgccggc
     2701 agctcaggcg cgcgctatat ctgccgcagt gaaagattct cacgaagccg cagaagtcgc
     2761 cacaaagcgc gacctgaatg atgtcagaac tgaactaaaa aacgacattg cagcccttga
     2821 tgcaaaaata gacaaactca gcctgcaact gaccgtcaga tttggtggca tgctcgtcgt
     2881 tgctgttggc gcactgacag ccatcatcaa attacctctt taaattacca gcccgttcgg
     2941 ggctggttta cctta
//
LOCUS       DADLSE010000274         2935 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLSE010000274 DADLSE010000000
VERSION     DADLSE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.276"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            246..1859
                     /locus_tag="JN692_004186"
     CDS             246..1859
                     /locus_tag="JN692_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7266292.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYFAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1910..2935)
                     /gene="ynaI"
                     /locus_tag="JN692_004187"
                     /pseudo
     CDS             complement(1910..2935)
                     /gene="ynaI"
                     /locus_tag="JN692_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
BASE COUNT          850 a          645 c          684 g          756 t
ORIGIN      
        1 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt
       61 gttcggtgtt ctatgtgctt tgccgcattt tggcgtctat tatttacact aaatctgatt
      121 tgatatattg atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt
      181 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
      241 aacttatgaa gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc
      301 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
      361 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
      421 ttcaggtcat tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg
      481 tccccggcgt cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc
      541 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
      601 ggcaacgtct ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg
      661 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca
      721 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
      781 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
      841 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag
      901 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc
      961 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacttcg
     1021 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     1081 atattccggg gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca
     1141 cgcaaaaagg gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc
     1201 gccgcctgat gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac
     1261 cagatgttac cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     1321 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     1381 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     1441 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     1501 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtgggtg
     1561 attataatga accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt
     1621 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     1681 ttaaagctcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     1741 ttgcaccaat ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc
     1801 ccattaataa ccctgaagat gtagcgtata gtcggactat gtatattgtg aagcattgat
     1861 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgaccct
     1921 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     1981 cgtgtgactg tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccact
     2041 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcagcaa
     2101 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     2161 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     2221 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     2281 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     2341 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     2401 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     2461 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     2521 tcatgccgaa atgttcgcca taaagcagaa caagcactac aataatggtg attttgataa
     2581 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     2641 actgattaat cagcagaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     2701 taaaatcgat aacgcctggg gtgataaatt tgagtttata gttttctata acataattaa
     2761 tgatgctacc aacagcactg ataattatgg tttagattaa aaattgcacc gcatggaata
     2821 aaaatccttt tcttttacga tttccacggc gaaaccaaaa gctcatcaga atcaatgctg
     2881 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagc
//
LOCUS       DADLSE010000275         2918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLSE010000275 DADLSE010000000
VERSION     DADLSE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.277"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..178
                     /locus_tag="JN692_004188"
     CDS             <1..178
                     /locus_tag="JN692_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266293.1"
                     /translation="GASLSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGK
                     INQTTTKMLFMCRE"
     gene            complement(286..687)
                     /locus_tag="JN692_004189"
     CDS             complement(286..687)
                     /locus_tag="JN692_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7266294.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            929..1222
                     /locus_tag="JN692_004190"
     CDS             929..1222
                     /locus_tag="JN692_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266295.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            1294..1953
                     /locus_tag="JN692_004191"
     CDS             1294..1953
                     /locus_tag="JN692_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7266296.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            2030..2491
                     /locus_tag="JN692_004192"
     CDS             2030..2491
                     /locus_tag="JN692_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7266297.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2698..>2918)
                     /locus_tag="JN692_004193"
     CDS             complement(2698..>2918)
                     /locus_tag="JN692_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HAY7266298.1"
                     /translation="ANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLK
                     EAAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT          758 a          607 c          721 g          832 t
ORIGIN      
        1 aggggccagt ctatcattac aaggctacga tcccagcgaa tttacattcg ctaacggtgt
       61 attttgcgat gttaaagaaa aattatgtcg tgatgatcgt tattttggtg tagatggtaa
      121 gcggagtgga aaaatcaatc aaaccaccac aaagatgtta tttatgtgtc gtgagtgatg
      181 ccttgtttac attatgttct ctgctattcg tcattaacct cttgagcgac agagaggagt
      241 ggggcgttaa cgtctatgac ggggatagtc cccgtcggtt gacatttact taatttgaat
      301 atcgacgtta tattttttag ttttgttttt acgggcgtcg tttcgagttt gcagcactcg
      361 cagctcatat ttgccagaag cagggagtga gtattgtccg tgactatcga gttctgggga
      421 atatctggat aaatcaacag aatcatcgat tcctggcccg aagagataag tatcagcgcc
      481 ttcattagag atactcacat gcactttctg accttttttg gcatagaagg tatatgtatc
      541 gtaatcgtat ccctttattt cgccggagta ctgagcgctg ctatggcctt tgctgaactc
      601 aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa gcagcaatac
      661 agccttactg atgctcttga ttttcattgg tagtctcctg ttcagaatga gtacgatgaa
      721 ctgttataat ataacaatcc cttacgggag aattccagtg gcttttctgc gttcggtaaa
      781 atcccggcgc ggcatttttt gccgggggga ttacttataa aggggcatgg actcaacaaa
      841 ccgcagaaat ctacagctgg ctggcttgtg gcgcgggttt cattgatgtt agcttatgcc
      901 aaaacctggc atcttaaaga gtaaaagtat gttttgtgtg atttatcgaa gcagcaagcg
      961 tgaccagacc tatttatatg tcgaaaaaaa agacgatttt tcgcgtgttc ctgaggaact
     1021 gatgaaaggt tttggtcagc ctcagttagc gatgattctg ccgttggatg ggcggaagaa
     1081 actggtcaat gctgatattg aaaaggttaa actggcatta accgagcaag gttactattt
     1141 gcagttaccg ccaccacccg aagatttgct gaagcaacat ctttccgtca tggggcagaa
     1201 aacagacgac actaacaaat aaccgatatc cggcggtggc attatctttg tcggcgcggg
     1261 ttttcatatc cacgataagg tgaggggaac gttatgtatc aacatcacaa ctggcaaggt
     1321 gcgctgctgg attatccggt gagtaaagta gtctgtgttg gcagtaacta tgccaaacat
     1381 attaaagaga tgggcagcgc agtgcccgaa gagccagtgc tgtttattaa accagaaacg
     1441 gcactgtgcg atctgcggca gccattggcg atcccatccg atttcggttc agttcatcat
     1501 gaagtcgaac tggcggtgtt gattggcgcg acgctgcgtc aggcaacgga agagcatgtc
     1561 cgcaaagcca ttgccggtta cggcgtggcg cttgatctga cattgcgtga tgttcaggga
     1621 aaaatgaaga aagccgggca gccgtgggaa aaggctaaag cgtttgataa ctcttgtccg
     1681 ctttccgggt ttatccccgc ggcggaattt accggcgatc cacaaaatac aacgctgggc
     1741 ctgagcgtaa acggcgaaca acgccagcaa ggtacgactg cggacatgat ccataaaatc
     1801 gttccgttga tcgcttatat gagcaagttt tttaccctca aggccggtga cgttgtgttg
     1861 acaggcacgc ctgatggcgt cggcccgttg caaagcggtg atgagctgac agtcactttc
     1921 gatgggcatt ctttgacaac tcgcgttttg taatactttt tgccgccaga aagcggcggc
     1981 aaaacttgca tcgctgtgcc agactggtta taaggtgcgt tttaacgtaa tggcggaaca
     2041 cctgatgagc gatgtacctt tctggcaaag taaaaccctg gacgaaatga gcgatgcgga
     2101 atgggagtcg ttgtgtgatg gttgcggtca gtgttgcctg cataaactga tggatgaaga
     2161 caccgacgaa atctacttca ctaacgtcgc ctgtcgccag ctcaatatta aaacctgtca
     2221 atgtcggaac tacgaacgtc gtttcgagtt tgaacccgac tgcattaaat taacccgtga
     2281 aaatctgcca acattcgaat ggctgccaat gacctgcgct tatcggttgc tggcggaagg
     2341 taaagattta cctgcgtggc atccgctact tactggttcg aaagcggcaa tgcatggtga
     2401 acgtatctct gtgcgtcata tcgcagtgaa agaatcagaa gtgattgact ggcaggatca
     2461 tatcttaaat aaacctgact gggcgcagtg ataaatttaa tagatgctga tgggggcttt
     2521 cttaatatac gtctggatgc tcgccatttc gtagccctgt ttttgaaagc gctctgtcat
     2581 aatgagagtt acccgatacc ttattatcaa caggtaactc tcaatcttta aaaaacgtct
     2641 gtgtttttaa atgtgataaa ccttatacct tgagtacatg gagagaataa tcgcttatca
     2701 gacttcaggt acctcaaaga gtgtcttttt accgtgtctt ttctgatact cattacaggt
     2761 gttaatcatt tttttggccg cttcttttag caaagaaagc attaaatcat cataatcaac
     2821 atagaatctg gttgtttcag tttccgtttt tatctccccg atggcggcta tttcggtggt
     2881 taatttcaat ttggcggcat cgatatcttt attcgctt
//
LOCUS       DADLSE010000276         2872 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLSE010000276 DADLSE010000000
VERSION     DADLSE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.278"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..1131)
                     /gene="traD"
                     /locus_tag="JN692_004194"
                     /pseudo
     CDS             complement(1..1131)
                     /gene="traD"
                     /locus_tag="JN692_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            complement(1559..1750)
                     /locus_tag="JN692_004195"
     CDS             complement(1559..1750)
                     /locus_tag="JN692_004195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266299.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            complement(1747..2169)
                     /locus_tag="JN692_004196"
     CDS             complement(1747..2169)
                     /locus_tag="JN692_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HAY7266300.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSEFVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMEVVLQALDGEHMS"
     gene            complement(2216..2641)
                     /locus_tag="JN692_004197"
     CDS             complement(2216..2641)
                     /locus_tag="JN692_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7266301.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIVFHPECNAIYAALD"
     gene            complement(2614..>2872)
                     /locus_tag="JN692_004198"
     CDS             complement(2614..>2872)
                     /locus_tag="JN692_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
                     /protein_id="HAY7266302.1"
                     /translation="RVRFAAAVTRSLLPVCRDFPVVHPLRFRGLTLQLPPAVCVRLRL
                     PLRPVHPRLIARLLWRHGTARCRGICEQRRKTSCNMRNLSL"
BASE COUNT          669 a          830 c          771 g          602 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 cgggttgatg tcacgcggaa taaactccgg agcgaccttc ggacgttcct gatatttcag
      181 tgagagtttg actgccggat acggtccggg cagggtgaca tagcaggtca gatccggcag
      241 ggactgaatg tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgttcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag cgaactccgc aatcttatgg ctgggagaac ggaaaaaggc
      421 acgggtgttc atgacgtcaa acagcgtggc agccgctttc tcaccgtaga tatcttccag
      481 ctgggcatag gactggatac caaacacata acagccaccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgtaa tgtgggtaac tcatcacaga agaaccacac
      601 acgacgatta cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat
      661 ggagatcacc ggcttcaggg aggcatgagt gtcggcattc gacgagataa acagccagcc
      721 gtttttctga tcctcccgga caccacgcat ccagtcacgg atggtgaagg gctcaccgtt
      781 atgctcaatc ccctgcaggt aacggatggc tttcacatag ttggtcagca cagcacggat
      841 ggagatggcc gttttctcga ttttctcttc caccaggttg gccgccggtg aattacgcag
      901 gtaggtacgc agtttttcaa ttttgatgga aagcagtgtg tccaccagtt tgctgtagct
      961 gcggttgggg tcattacgca tcagatacgc cgcttccgca aaaatggtac gtcctgaacc
     1021 ctgccagaac gggtcctctt ttgtgcccat cgggatcaga gtatttgcgg tattatcaaa
     1081 atccggctgt gtcaggcact ccttccacaa atcccaggcg gcacagcgtg caccggggcg
     1141 gttcagtagt gaaaccggct ctcagtattg ggggcttata aaggcttgag cggagtgcgg
     1201 ggaaactcgc acgctccgtt cttagggggc ggcggcgcag caatgtgccg ctgctacccg
     1261 acccgccagg gcggagacag cttcgctggc tccgttcacg acagccagcc ccgccaaggg
     1321 gacgcccggg aaagccataa acaggagagg agaggaacgg aaagaagaag aggaagaaga
     1381 acaataacag gcacggtaac cacagaaaaa taacatcaga aaaaaatatt ccggacacat
     1441 cataaacggg aagggtaacg ggcgacgcgt cagcgtggcc cgcttccctt ccggcaggcg
     1501 tcagcctgcc aggggttttc gcgcgggctg acaggcggcc tccaccgcct gccctgtatc
     1561 acctgtgcct gctgccctga aggatgtagt gaccaccgag gaaaaccgcc tgctgattcg
     1621 ccacaaaatc gcagatttca tcaacaatct gcgcggtggt gagcggtttc tgccccattt
     1681 cacgacgccg cgcattaatc acggccctgt aattacgcag aatttcgcgg gtttctgtgc
     1741 tgatattcat gacatgtgct ccccgtccag cgcctgtaac acaacctcca ttgcttcgcg
     1801 ttcctccctg acgaatgcgt caccgtcgcc cccgttctca ctcccgacgg catacaggcg
     1861 cttcatttca ctgcctgcca gcgccatcac tttttccagc atcaccgcag ggactgttac
     1921 aagacggttt acgcgctttt cctccatcag ttcattgaca atatcggaaa caactctgtc
     1981 acatgcgcca tatacaaaag catcgtccag ccgctgcatg acggtttcca gctcatcgtc
     2041 tgtcagactc cagtcctggg ctatgtgacg cacatcccca cgggtcagca ccacggcaaa
     2101 aacaaattca ctgtccggca cgtctgtatc cagtacatcg cggataattt ctttaacagt
     2161 acagtacatg ttctcgtctc ctttacaggg gcggcattgc cgccccgtcc gttaatcagt
     2221 ccagtgccgc gtaaatcgcg ttgcattcag ggtgaaatac gatgtgacgc cacaactgcg
     2281 catcgcgcat ccggtaaagc tgggtcagtc ctgcatcccc gctgtcgtgg tacagccaca
     2341 actggcggtt aatcacaaga gaagtcagaa tgatgcctgc ggcatcagca ctcacggtac
     2401 ggtcaaacca gtttgcaccg ttcaccatgt ggaaacggtc gccatccggc atcatgtaac
     2461 cgccaccgtc cggcaggcgg atgaagtgcc agaagccgcc ttcatattcc ggcatcattt
     2521 ttttcgccag cgcgtacaca tacgcctcgg catacagaaa atcgttgcca aacaggtaag
     2581 gcagaaagga gagacggtcg cactcttcaa cattcagagt gacaggtttc gcatattgca
     2641 tgatgttttc ctccgttgtt cacatattcc gcgacaccgg gcggtgccgt gcctccataa
     2701 aagccgggcg ataaggcggg gatgcacagg gcgcaaggga agccgcagcc tgacgcacac
     2761 ggctggcggg agttggaggg tgagaccgcg aaacctgagc gggtgaacga ccggaaaatc
     2821 acggcagacg ggaaggaggg accgggtgac cgcagcggcg aagcgtaccc tt
//
LOCUS       DADLSE010000277         2848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLSE010000277 DADLSE010000000
VERSION     DADLSE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.279"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(254..1051)
                     /locus_tag="JN692_004199"
     CDS             complement(254..1051)
                     /locus_tag="JN692_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7266303.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN692_004200"
     CDS             complement(1061..1612)
                     /gene="ybcL"
                     /locus_tag="JN692_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7266304.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKISVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN692_004201"
     CDS             complement(1781..2113)
                     /gene="emrE"
                     /locus_tag="JN692_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7266305.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN692_004202"
     CDS             complement(2458..2772)
                     /gene="fliE"
                     /locus_tag="JN692_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7266306.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
BASE COUNT          840 a          637 c          552 g          819 t
ORIGIN      
        1 cctttaatta tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagtccaa
       61 acgccggatg caaaaaaaat cacacgtgca tctctgaaat tgtgatggag gaagtgagta
      121 tcaattatat gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc
      181 tccaataggt tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
      241 taataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atcttaaaac
      301 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
      361 cgctgatggg ttttactgtc gtttgtatag cagacaacgc attatttaac ctcacgtcgt
      421 ccaatatact ttgaaaactt attccctcgc ttgccagacg gcgatgcaat gtggaaacag
      481 aaatgtagag atatctcgct accttgtttg ctgtccattt tgttccaggt tcggatagca
      541 ttaggttata acaacgactt attaatgatt gtttactatc tgataaaagt aaacaattaa
      601 catgatgtgc tcctaatgaa agtagaacgc ccattgctaa gtgttcctga atttgcgttg
      661 agaagcctcg ggaaatagat gtttttagtt gctcccaaca atatattaac tcggggttct
      721 gaggtaagca gaaacctgtc gtgttacgaa tttgatcagt tatcgcataa aatttttgaa
      781 acttttcaat tagatcaatg ggataataaa gcatttctgc aagataaaga cctgcttctg
      841 gataattcac aatataaaat tcatatccac agggaaataa tattatctga ttattatcaa
      901 cagttagact atgcgtctcc caattgataa ctttttttcc ctgacggata cgacataaag
      961 ctggcataag aggcttaacc ctgtgaatct catgatgttt atgcatccgt atttcttcaa
     1021 tctttaggtt agacttatct tttgccagca tactcttacc ctactttatc tcataaactg
     1081 gtgttatctc agcggttgca attttattgg cattaagcat ataaccaact aacgctccgc
     1141 tggaattaga atccacagaa atcttatcag tttttagagc ccatacttta aactggtaat
     1201 gatggggttt atctcctttg ggaggacatg ctccaccaaa tcctgcataa ccaaaatcat
     1261 ttcggccttg aacagcacca gtcggaagtt ttgtaccatc acgtcttcct gcatcagtgg
     1321 gcaaatatgt tacagttgct ggaatattag caacagtcca gtgccaccat ccactgcctg
     1381 taggagcatc tggatcatac acagttacgg caaagctttt ggtgccttca ggagcaccag
     1441 accaggttaa tgagggcgat gtattaccac cttcacaccc aaatccagaa aatacatgag
     1501 acgttgtaag ttgctctcct gtttttattt cattactagt gacctgaaat gctgcggcct
     1561 gcgcagaaaa tgttatgaat gccaatacac ttgaaacgat aagttttttc ataaaaacct
     1621 cattgttgtg acctatcgtt attttatttg atgtgccttg atctcattat gcataaaggc
     1681 gcaatgatcg cgcaaatgca atcataatta taaaggtgcc ccgaacatta gccataatat
     1741 gcacaaatca gattcctgtt atctcaagga taattatttt ttaatgcggt gcacttcgtg
     1801 acaacaagtt aatgactaac acaccggcac aaatcaacat catgcctatg atagctggca
     1861 ggtccagtcg ttgaccaaaa aatccccagg aaagcaaact aatcagaacg ataccgactc
     1921 ctgaccagat agcataagca atccctgtag gtatataagc cagcgtctga gctaataacc
     1981 agaatgatgc acaatagcaa ataattgtgc caacagaggg ccataaccgt gtaaacccct
     2041 ctgaaaactt cattaaggtt gtacctatga cctctgcaag tattgcgcca ccaagataaa
     2101 tataagggtt catagcatat tctttcctgt tcatactgga gagaattgta ctacagtttg
     2161 aacacaactc accgttttca tcctggtgta tgtgtatcca aggggctctg gcgtggcaag
     2221 agataaaggc aggaacaata atgatcagaa ggaaccccag gctaacgaat gagttcactc
     2281 ttcttgggag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
     2341 aaacaaccag gatcaggtat ccattttaaa ccagaaattg agtgagtttg ccccatccat
     2401 gccccacgct gtgggatcgg atataattaa acaagcatag tcaacaggtt acacaaccta
     2461 cacctgcatg gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc
     2521 catttgcata gaaactgagg ctttttgcat atcggtcatc acatcgttta gcgccacgcc
     2581 gggttcaccg agggtgaatt tttctgcctg cgtgcgggcg actgtttgtg tatcgcttat
     2641 gcgatcgagc gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgattc
     2701 ctgcgcacgc gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg
     2761 tatcgctgac attttcgtct cccggataat ttctggtaac aaagcctacc agtaagtcaa
     2821 taagacaaag gcgctaaata gcaacaaa
//
LOCUS       DADLSE010000278         2783 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLSE010000278 DADLSE010000000
VERSION     DADLSE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.280"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="JN692_004203"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="JN692_004203"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7266307.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(1698..>2783)
                     /locus_tag="JN692_004204"
     CDS             complement(1698..>2783)
                     /locus_tag="JN692_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7266308.1"
                     /translation="FATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          737 a          651 c          672 g          723 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgagccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc gttatggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg gcgtgaaatt
     1021 ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc gtatcatcga
     1201 agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca ttgcgtatga
     1261 acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc tgatgctgcg
     1381 tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc tggagctgcg
     1441 ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaagccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta ttggtcaatt
     1561 gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt atgccaaaac
     1621 tggcctgctt tcctcctctg ccacttcagg cgtgccgcag gtggagaata tggaaaacaa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg cttatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat attgttttcc
     1921 gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat cgggacatga
     1981 tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtca
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg taatgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc aacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata gttgagattg
     2761 aaaaacggcg tataggtagc aaa
//
LOCUS       DADLSE010000279         2768 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLSE010000279 DADLSE010000000
VERSION     DADLSE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.281"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            37..393
                     /locus_tag="JN692_004205"
     CDS             37..393
                     /locus_tag="JN692_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HAY7266309.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            493..1707
                     /locus_tag="JN692_004206"
     CDS             493..1707
                     /locus_tag="JN692_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266310.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            1934..>2768
                     /locus_tag="JN692_004207"
     CDS             1934..>2768
                     /locus_tag="JN692_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7266311.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VR"
BASE COUNT          671 a          614 c          606 g          877 t
ORIGIN      
        1 tatattttat tgtttatttc atgcagccat tacacgatga acgcttatga actccaggca
       61 ctccgccata tttttgccat gactattgat gaatgcgcga cctggattgc ccagaccggt
      121 gacagtgaaa gctggcgtca gtgggaaaat ggcaaatgcg ccattcctga ttgtgtagtc
      181 gagcaactgt tggctatgcg tcagcaacga aaaaaacatc ttcatgccat catagagaaa
      241 ataaataacc gcatcggcaa caacaccatg cgttttttcc ccgacttaac cgcgtttcaa
      301 caagtctacc ctgatggcaa ttttattgac tggaagatct atcaatcggt agccgctgaa
      361 ttatatgcac acgatctgga gcggctttgt taatttttcc acagaaagga attgtcgttg
      421 ttacaacaat aatgaatgga tactgacaca acatcgctac actttttaaa gcacctttgc
      481 taagtagaac ctatgaaaaa tccctattac cctaccgcac ttgggttgta ttttaattac
      541 ctggtgcatg gtatgggcgt cattttgatg agcctgaata tggcctcgct ggagacactt
      601 tggcagacta atgccgcggg tgtctcgata gttatctcat cgctgggcat tggtcgatta
      661 agtgtcttgc tttttgcagg attattatcc gatcgctttg gtcgccgccc ttttatcatg
      721 ctcgggatgt gctgctatat ggccttcttt tttggcatcc tgcagaccaa taacatcatt
      781 atcgcttatg tttttggctt tctggcggga atggcaaaca gttttctcga tgcaggcact
      841 tatcccagtt tgatggaagc ttttccacgc tcacctggga cagccaatat tttaattaaa
      901 gcatttgttt ccagcggaca atttttatta ccgctaatca ttagcctgtt agtgtgggct
      961 gaactgtggt tcggttggtc ctttatgatt gctgcaggca ttatgtttat taacgctctg
     1021 tttttatacc gttgtacgtt cccaccccat cctggtcgtc gcttacctgt cataaagaaa
     1081 accaccagct ctacggaaca tcgctgttca attatcgatt tagccagtta taccttatac
     1141 ggctatatct caatggcaac gttttatctg gttagccagt ggctggcaca gtacggacaa
     1201 tttgttgcag gcatgtcata cactatgtcg atcaaactac tcagtatcta caccgtgggt
     1261 tcgctgcttt gtgtatttat taccgctcca ctcattcgta ataccgttcg cccaacaaca
     1321 ttactgatgc tgtacacctt tatctcattt attgctctgt ttaccgtctg cctgcatccc
     1381 acattttatg tggtgataat atttgctttt gtcattggtt ttacctctgc tggaggtgtt
     1441 gtgcaaattg gcctgacgtt aatggctgaa cgtttccctt acgctaaagg taaagctaca
     1501 gggatctatt acagtgcggg cagtattgcg acctttacta ttccgttgat tacggctcat
     1561 ctgtcccaaa gaagtattgc cgatattatg tggttcgata ccgccatcgc tgccatcggt
     1621 tttttactgg cactgtttat cggcttacgc agccgcaaaa aaacgcggca tcactcgcta
     1681 aaggaaaatg tcgctccggg tgggtaatgc aatattcttt tcaggtcatg caagatctta
     1741 cggataaata actctttctg cgctaactaa ggaaaatcgc gatcaaaaac aaactatgac
     1801 atgcaatatt cctggaaaca taaactttat gccatgtacc cagggaaaat catcttcagt
     1861 atagtaatta tgtaaaccgt cggagaacaa tacgtacggt aacgaaatta tctttcagca
     1921 aggagctgtg aaaatgtctc aaaataaggc tttcagcacg ccatttatcc tggctgttct
     1981 ttgtatttac ttcagctatt tcctgcacgg cattagtgtt attacgcttg cccaaaatat
     2041 gacatctctg gcagaaaagt tttccactga caacgcgggc attgcctatt taatttccgg
     2101 tatcggtttg gggcgattga tcagtatttt attcttcggt gtgatctccg ataagtttgg
     2161 tcgtcgggcg gtgatattaa tggcagtaat aatgtatctg ctattcttct ttggtattcc
     2221 cgcttgcccg aatttaactc tcgcctacgg tctggcagtg tgcgtaggta tcgctaactc
     2281 agcgctggat acgggtggct accccgcgct catggaatgc tttccgaaag cctctggttc
     2341 ggcggtcata ctggttaaag cgatggtgtc atttgggcaa atgttctacc caatgctggt
     2401 gagctatatg ttgctcaata atatctggta cggctatggg ctgattattc cgggtattct
     2461 gtttgtactg atcacgctga tgctgttgaa aagcaaattc cccagccagt tggtggacgc
     2521 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
     2581 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
     2641 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
     2701 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
     2761 ggtccggc
//
LOCUS       DADLSE010000280         2702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLSE010000280 DADLSE010000000
VERSION     DADLSE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.282"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..407
                     /locus_tag="JN692_004208"
     CDS             <1..407
                     /locus_tag="JN692_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266312.1"
                     /translation="ADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVED
                     VDNADINQRLLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAAL
                     VYKIFCISESNDARECAAPGAVACRDCGETNA"
     gene            583..762
                     /locus_tag="JN692_004209"
     CDS             583..762
                     /locus_tag="JN692_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7266313.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREVEVGHEH"
     gene            752..1693
                     /locus_tag="JN692_004210"
     CDS             752..1693
                     /locus_tag="JN692_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7266314.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQNDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPEGFRG"
     gene            1788..2441
                     /locus_tag="JN692_004211"
     CDS             1788..2441
                     /locus_tag="JN692_004211"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY7266315.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDSDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2438..>2702
                     /locus_tag="JN692_004212"
     CDS             2438..>2702
                     /locus_tag="JN692_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7266316.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGQKFQSAACAAI
                     IEQLRRLPKPTSAHASVEIVLFPPDNRTRDLDNYNKALFDALTH"
BASE COUNT          678 a          652 c          764 g          608 t
ORIGIN      
        1 gtgccgatgg cgatcagatt ttcccgctgg gatgggcaat gattttgcaa cgtgctggtg
       61 gaactcactt cattgctgac gctgtggcgc agtctgcaaa tggcgtcttt gtgtctcttc
      121 ctgacgtcga ggatgtggac aacgccgata ttaaccagcg cctgctggaa gtcattgaac
      181 agatcggcag ttattcaaaa cagattcgtt cagcaattga agacggtgta gtggaaccgc
      241 atgagaagac agcaattaac gacgagctgt acctctcaat ttcgaagctg caggagcatg
      301 cagcactggt ctacaaaatt ttttgcattt cagaaagtaa tgacgcccgc gagtgtgcag
      361 ctccgggcgc cgtggcgtgt cgtgactgtg gagaaactaa cgcatgaaca gtttaacaac
      421 acactaccgt cgctcgcaac tgattgcgct tcctgtaccg ggtggaaaag cgaaggtgga
      481 gtattgctat gcagtaaatg taccaggtga cagggaaatt gtaacccaca gctttgcaga
      541 gtgggctgtg ggtgatttca accggcagaa ggagacagtc ctttgcgaca agttaaccgc
      601 tggttcaaag atcactacgg agtgcccgtc agagtcattc gttgggagcc ggaaacacaa
      661 cgggttatct acctccgcga aggctatgag catgagtgct tcagcccgct cgaacagttt
      721 cgtcgtaaat tcagggaagt agaggtcggt catgagcact aaattaaccg gctatgtatg
      781 ggatggttgc gctgcatcag gcatgaaatt atccagcgtg gcaattatgg cccgcctggc
      841 tgatttcagt aatgacgaag gtgtgtgctg gccatcaatt gaaaccattg cccgtcagat
      901 tggcgcgggg atgagtaccg tcagaacggc tatcgcacgg ctggaagcag aaggctggtt
      961 aacgcgtaag gcgcgtcgcc agggtaaccg caatgcgtcg aatgtttatc agcttaacgt
     1021 tgcgaagctt caggcagcgg cattttctca actgtcagat tctgacccgt caaaatctga
     1081 cgcatcaaaa tctgacccgt caaaatttga tgcgtcgaaa tctggcaaaa aagcgggttt
     1141 tcacccgtca gaatctggcg gggatccgtc agtaaaatca aaacatgatc cgtcagataa
     1201 aaaaccttct cgtccggacg cttcgcaacc ggacacgcag acggctgaac aggatttttt
     1261 aactcgccat cctgatgcgg ttgtattcag ccctaaaaag cgccagtggg ggacgcagaa
     1321 tgatttgacc tgcgcacagt ggctctggaa aaaaatcatc gccctgtacg agcaggctgc
     1381 cgaatgtgac ggcgaggtgg ttcgtcccaa agaaccgaac tggacagcct gggcaaacga
     1441 aattcgcctg atgtgtgtgc aggatggtcg tactcacaaa caaatctgcg agatgtacag
     1501 ccgcgtcagc cgcgatccgt tctggtgccg taacgtgctc agcccgtcga agctgcggga
     1561 aaaatgggat gagctttccc tgcgcttatc gccgtccgtc agcacgtaca cagaaaaacg
     1621 cgaagacccg tacttcaaag ccagttacga caacgtggac tacagccaga tcccggaagg
     1681 attcaggggg tgatcatgag tcttttgaat gaagttcaga aattcattga agcccatccg
     1741 gggtgtactt ccggagacat tgcggatgct ttttacgtgg gggcttaatg agtaataaat
     1801 attgccaggc gctggtagaa ctgcggaaca aaccagccca tgaactgaag gaagtgggcg
     1861 atcagtggcg cacgccggac aacattttct ggggaattaa caccttgttt ggtccgtttg
     1921 ttctggatct gttcactgac agtgataacg ccaaatgtgc cgcgtattac acggcggaag
     1981 acaacgcgct ggcgcatgac tggtcagaac gtcttgcgga gcttaaaggt gctgcctttg
     2041 gcaatccccc gtacagccgc gccagtcagc atgaggggca atacatcacc ggcatgcgtt
     2101 acatcatgaa gcatgccagt gccatgcgtg ataaaggcgg gcgctatgtt ttcctgatca
     2161 aagctgccac cagcgaagtg tggtggccgg aagatgcaga tcatattgct tttattcgcg
     2221 ggcgtattgg ttttgaactg cctgcctggt ttatcccgaa agacgaaaag caggtgccaa
     2281 caggtgcttt cttcgctggt gctattgctg ttttcgacaa gacctggaag ggaccggcaa
     2341 tcagctacat cgggcgcgat gaacttgagg catgtggtga ggcatttctg gcgcaggttc
     2401 gccagcaggc ggaaaaactg gtcagggaga tggtggcatg aagctaatac tgccttttcc
     2461 gcccagcgtg aacacgtact ggcgacaccc caacaaaggg gcatttgctg gtaagagcct
     2521 gataagcgag gcggggcaaa aatttcagag cgcggcgtgt gccgccatca ttgagcagtt
     2581 acgtcgtctg ccgaaaccaa cgtcggcaca tgcttcagtg gagatcgtgt tgtttcctcc
     2641 ggataacagg acccgcgatc tggacaacta taacaaggcg ctgtttgacg ccctgaccca
     2701 cg
//
LOCUS       DADLSE010000281         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLSE010000281 DADLSE010000000
VERSION     DADLSE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.283"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN692_004213"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN692_004213"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7266317.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN692_004214"
     CDS             complement(1725..2096)
                     /locus_tag="JN692_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7266318.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN692_004215"
     CDS             complement(2119..2463)
                     /locus_tag="JN692_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7266319.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          668 a          648 c          672 g          709 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccat ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacaat ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLSE010000282         2617 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLSE010000282 DADLSE010000000
VERSION     DADLSE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.284"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            257..346
                     /locus_tag="JN692_004216"
                     /pseudo
     CDS             257..346
                     /locus_tag="JN692_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            370..>2617
                     /locus_tag="JN692_004217"
     CDS             370..>2617
                     /locus_tag="JN692_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7266320.1"
                     /translation="MGTGLAKADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFRDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSREGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNV"
BASE COUNT          627 a          658 c          685 g          647 t
ORIGIN      
        1 gcatcatttc tctgcgctta tcgagatact gagcatggtt gtaaattcag ctaacccagt
       61 gcgttgacaa aaaattagcg caagaagaca aaaaatcacc ttgcgctaat gctctgtatc
      121 aggtcactaa taccagctaa gtagttgatt catagtgact gcatatgttg tgctttgtcg
      181 cattttgtag tctattattt aatctgtaac aacgataata caatgaattt taattggttt
      241 ttgtgatttt gtccttcttc tgacatggaa ggacacccga tggataccca acgattccaa
      301 tcccaatttc attggcattt atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc
      361 ctgtctcttg tgggaacagg gcttgctaaa gctgatgatt cgcttccttc cagtaactat
      421 gcgccgcccg ccgggggaac attctttttg cttgctgaca gcagttttag cagcagtgaa
      481 gaggcgaaag tgcgactgga agcgccgggg cgtgattatc ggcgctatca gatggaagag
      541 tacggcggcg tggacgttcg cctgtatcgt attcctgacc cgatggcatt tttgcgccag
      601 cagaaaaacc tgcatcgcat tgtggtgcaa ccgcaatatc tgggcgacgg gctgaacaat
      661 acgctgacct ggctgtggga taactggtac ggcaaatctc gccgcgtgat gcagcgtact
      721 ttctcttctc agtcacggca gaatgtgact caggcattac ccgaattaca tctcggcaat
      781 gccattatta aaccttcccg ttatgtacag aacaaccagt tttccccgct gaaaaaatat
      841 cccttggtgg aacagttccg ttatccacta tggcaggcta aaccggtcga gccgcagcaa
      901 ggggtaaaac tggaaggcgc atccagcaat ttcatctcgc cgcagccggg taatatttat
      961 attcctctcg gccaacaaga gccggggctg tacctcgtcg aggcgatggt aggtgggtat
     1021 cgggcgacga cggtggtgtt tgtttccgat accgtggcgc ttagcaaagt gtcaggtaac
     1081 gagcttctgg tatggaccgc gggtaaaaaa cagggtgaag cgaagcccgg ctcagagatc
     1141 ttgtggactg acggtcttgg cgtgatgacc cgcggggtga ccgatgacag cggtactttg
     1201 cagttacaac atatatcgcc agaacgttca tacattctgg gtaaggatgc tgaaggcggc
     1261 gtttttgtct ccgagaactt cttctacgaa agcgaaatct acaacacccg cttgtatatt
     1321 tttaccgatc gcccgctata tcgcgcaggc gatcgtgtcg atgttaaagt gatgggccgc
     1381 gagttccgcg atccgttgca ttcatccccc atcgtcagcg ccccggcgaa gctttcggtg
     1441 ctggacgcta acggcagtct gttgcaaacc gtcgatgtca cgctggatgc gcgcaatggc
     1501 gggcagggaa gtttccgcct gccagaaaat gccgttgccg gaggttatga gttacgtctt
     1561 gcttaccgca atcaggtcta tagcagcagt tttcgcgtgg caaactacat caagccacat
     1621 ttcgagattg gtttagctct cgacaaaaaa gagttcaaaa ctggcgaagc ggtcagcggc
     1681 aaactgcaac tgctctaccc ggatggcgag ccggtaaaaa atgcccgcgt gcagttaagt
     1741 ttgcgcgctc agcaattatc aatggtcggt aacgatttgc gttatgccgg acgtttcccc
     1801 gtgtcgcggg aaggcagcga aacggtgtcc gacgccagcg gtcatgtggc gttaaatctc
     1861 cccgccgccg ataaaccgag ccgctatttg ttaaccgtct ccgccagtga cggcgcggcg
     1921 tatcgcgtca ccaccaccaa agagatcctc attgaacgcg gcctggcgca ttactcttta
     1981 agtaccgccg cacaatacag taatagcggc gagtcggttg tgttccgtta tgccgcgctg
     2041 gaatcttcaa aacaggttcc tgttacgtat gaatggttgc gtctcgaaga ccgcacgagc
     2101 catagcggag agctaccgtc aggcggcaaa tcctttaccg tcaatttcgc taaacctggc
     2161 aactacaatc tgacattacg cgataaagac ggcttaattc tcgctgggtt aagtcatgcc
     2221 gtcagcggta agggcagcac ggcgcatact ggtacggtag atatcgtggc ggataaaacg
     2281 ctgtaccagc caggcgaaac cgcgaagatg ctgattactt tcccggagcc aattgatgaa
     2341 gcgttattga cgctggaacg cgatcgcgtg gaacagcagt cgctgctttc gcatccggca
     2401 aactggctaa cgctacaacg tttaaacgat acccagtatg aagcccgggt tccagtgagc
     2461 aattcctttg cgcctaacat cactttttcg gtgctgtata cccgtaatgg tcagtacagt
     2521 tttcagaacg ccgggatcaa agttgccgtt cctcagcttg atatccgggt gaaaacggac
     2581 aaaacccatt accagcctgg tgaactggtc aatgtcg
//
LOCUS       DADLSE010000283         2599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLSE010000283 DADLSE010000000
VERSION     DADLSE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.285"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            176..355
                     /locus_tag="JN692_004218"
     CDS             176..355
                     /locus_tag="JN692_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266321.1"
                     /translation="MTLVSVLATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDF
                     MMACDMYFRWGHFNK"
     gene            complement(548..635)
                     /locus_tag="JN692_004219"
     tRNA            complement(548..635)
                     /locus_tag="JN692_004219"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(599..601),aa:Ser,seq:gga)
     gene            869..1807
                     /gene="ghrA"
                     /locus_tag="JN692_004220"
     CDS             869..1807
                     /gene="ghrA"
                     /locus_tag="JN692_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HAY7266322.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGCKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            1862..2599
                     /locus_tag="JN692_004221"
     CDS             1862..2599
                     /locus_tag="JN692_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7266323.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHLVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
BASE COUNT          629 a          593 c          662 g          715 t
ORIGIN      
        1 gctgcggcag taacaccgat gcgttcagca agcttcaggg cttcactgcg aaattcaggc
       61 gaatgctgtt tacggggttt tttactggtt gatactgttt ttgtcatgtg agtcacctct
      121 gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat catggattac
      181 gctggtgtcg gtattggcaa cgcttgtgga atggcggatt tggtttgaag cctatttttt
      241 actctgctat gtaccgtgct taatttacta ttttttccat tgtcgattcc tctggcataa
      301 cgattttatg atggcatgtg acatgtattt ccgttggggg cattttaata agtgaggaag
      361 ttacaggaag tgaccagata acgcatatat gttctgtact atcttgcgca ttttgattgt
      421 tgactgagta accagacagt tgatgtgcac aatttcccct cgccctaaca gacgtgggcg
      481 ggggcacccg gtgtgtacaa tatacgaaaa aaaagcccgt actttcgtac gcgctcttct
      541 ttaaatatgg cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc
      601 caggcgtgct ccttcagcca ctcggacacc tcaccaaatt gtcgttcctg tcttgctgga
      661 acgggcgcta atttagggaa atcatgacct gaggtcaaca aactttttga aaaaatcgcg
      721 cgtttattca aacttcaatc aatgtgtggt tttaataagc gaaatctgct ttttttgcca
      781 ccgaccacgg atttgttatg ctggtggcct ttgtagatca taacgataag tgcgaataaa
      841 tttcgcacaa cgcttttcgg gagtcagtat ggatatcatc ttttatcacc caacgttcga
      901 tacccaatgg tggattgagg cactgcgcaa agctattcct caggcaagag tcagggcatg
      961 gaaaagcgga gataatgact ctgctgatta tgctttagtc tggcatcctc ctgttgaaat
     1021 gctggcaggg cgcgatctta aagcggtgtt cgcactcggg gccggtgttg attctatttt
     1081 gagcaagcta caggcacacc ctgaaatgct gaacccttct gttccacttt ttcgcctgga
     1141 agataccggc atgggcgagc aaatgcagga atatgctgtc agtcaggtgc tgcattggtt
     1201 tcgacgtttt gacgattatc gcatccagca aaatagttcg cattggcaac cgctgcctga
     1261 atatcatcgg gaagatttta ccatcggcat tttgggcgca ggcgtactgg gctgtaaagt
     1321 tgctcagagt ctgcaaacct ggcgctttcc gctgcgttgc tggagtcgaa cccgtaaatc
     1381 gtggcctggc gtgcaaagct ttgccggacg ggaagaactg tctgcatttc tgagccaatg
     1441 tcgggtattg attaatttgt taccgaatac ccctgaaacc gtcggcatta ttaatcaaca
     1501 attactcgaa aaattacctg atggcgcgta tctcctcaac ctggcgcgtg gtgttcatgt
     1561 tgtggaagat gacctgctcg cggcgctgga tagcgggaaa gttaaaggcg caatgctgga
     1621 tgtttttaat cgtgaaccct taccgcctga aagtccgctc tggcaacatc cacgcgtgac
     1681 gataacacca catgtcgccg cgattacccg tcccgctgaa gctgtggagt acatttctcg
     1741 cactattgcc cagctcgaaa aaggggagag ggtctgcggg caagtcgacc gcgcacgcgg
     1801 ctactaataa agcatcagga ttcctgctat ccttggcggg aattgaatac aggagagagt
     1861 tatgtatccc gtcgaccttc atatgcatac cgttgccagc acacatgcat atagcacatt
     1921 gagtgattac attgcccagg ccaaacaaaa gggcattaaa ctttttgcga tcaccgatca
     1981 tggcccggat atggaagatg cgccgcatca ttggcacttc attaacatgc gtatctggcc
     2041 gcgagtggtt gatggggtag ggatcctgcg cggcattgaa gctaacatta aaaatgttga
     2101 tggtgaaatt gactgcagcg gtaaaatgtt tgactcgctg gatctgatta ttgccggttt
     2161 tcatgagccg gtttttgcgc cacatgacaa agcgaccaat acacaagcga tgatcgccac
     2221 tatcgccagc ggcaatgtgc atataataag ccatcccgga aatccgaaat atgaaataga
     2281 tgttaaagcc gttgctgaag cagccgcgaa acatctggtg gcgctggaaa tcaataattc
     2341 ctcattttta cactcacgta agggcagtga agacaactgt cgtgcggtag ctgcagcggt
     2401 acgtgatgct ggaggttggg tggcattagg ctcggattct cacaccgcgt ttaccatggg
     2461 ggaatttgaa gagtgtctta aaatcctcga cgcggtagat tttccgccag agcgcatttt
     2521 gaatgtttct ccgcgccgct tactgaactt ccttgaatct cgcggtatgg caccgattgc
     2581 ggaatttgca gacctttaa
//
LOCUS       DADLSE010000284         2527 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLSE010000284 DADLSE010000000
VERSION     DADLSE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.286"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..653)
                     /locus_tag="JN692_004222"
     CDS             complement(<1..653)
                     /locus_tag="JN692_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7266324.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFY"
     gene            complement(805..1278)
                     /locus_tag="JN692_004223"
     CDS             complement(805..1278)
                     /locus_tag="JN692_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7266325.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            1434..2447
                     /gene="intI1"
                     /locus_tag="JN692_004224"
     CDS             1434..2447
                     /gene="intI1"
                     /locus_tag="JN692_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7266326.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
BASE COUNT          543 a          703 c          687 g          594 t
ORIGIN      
        1 taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat acctttgatg
       61 gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgccgattat ccgtttcagt
      121 ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca
      181 gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc gggcgcttta
      241 tccgtgttga tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat tttacccaga
      301 aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac agtgcggccc
      361 cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac gtaggtttca
      421 tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag ccgtttttcc
      481 atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac attcactccg
      541 cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt
      601 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
      661 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcccaat
      721 attcacataa cgtcgctatc aatcgcggaa aaggcgtagt gcgcgaagcg aacgaagctt
      781 tttgccgtcg attgcatagc tttgttaacc ctttttccaa atttgatagc aatagttaat
      841 gtttgaagta aagtgttgct caaaaacaac ttcgaaggta tttggaatac tcgggaagaa
      901 aacatccccc tctggctcga tgtcgatcgt cgataagtgg agcgtagagg ccatgggtaa
      961 tgtttctcgg taaatttctc cgccaccaga cactataacg tgaccggtga attcagctag
     1021 cctgtccatg gcctcttcga ttgactgaaa tacaactaca ttgtcatcat ttgatgtcca
     1081 acctgagcgg gtaacgaccg cgtatttcct attggggagt gcgcccatag attcaaacgt
     1141 cttgcgaccc accagaagcc actgattgta ggtcaatgct ttaaaaagta gctgctcccc
     1201 tttcgcggac cagggtatgt ctggaccgca accaatcacg ccgtttttcg ctttcgcagc
     1261 catcaatgat actttcaagg ttctcatcct gggttaactt tgttttaggg cgactgccct
     1321 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     1381 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
     1441 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
     1501 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtccac tgggttcgtg
     1561 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg
     1621 catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat
     1681 tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg
     1741 agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg
     1801 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa
     1861 cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg
     1921 gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga
     1981 gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc
     2041 aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg
     2101 ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac
     2161 ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac
     2221 gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt
     2281 tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc
     2341 attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag
     2401 tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc
     2461 aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt
     2521 tgcggaa
//
LOCUS       DADLSE010000285         2481 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLSE010000285 DADLSE010000000
VERSION     DADLSE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.287"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            135..564
                     /locus_tag="JN692_004225"
                     /pseudo
     CDS             135..564
                     /locus_tag="JN692_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            751..915
                     /locus_tag="JN692_004226"
                     /pseudo
     CDS             751..915
                     /locus_tag="JN692_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1409..1987
                     /locus_tag="JN692_004227"
     CDS             1409..1987
                     /locus_tag="JN692_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266327.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2096..2354)
                     /locus_tag="JN692_004228"
                     /pseudo
     CDS             complement(2096..2354)
                     /locus_tag="JN692_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2338..2481
                     /locus_tag="JN692_004229"
                     /pseudo
     CDS             2338..2481
                     /locus_tag="JN692_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          751 a          451 c          634 g          645 t
ORIGIN      
        1 attcctgccg gtatcagcgc cgacggcatg tattgccctg aatgcggtac ggttcactgg
       61 ccggatggcg ttattccgcc tttctgatgc ttccccgttt tgccgatatt tttcagcagg
      121 gaaaccgatg gcttaactgg ctggagaagc agccggtgca gatgagccgc ctggaacatt
      181 atgccggtca ggatgaaatt ggcctgcgtt acaacagcca ccggacaaaa cgagaagaaa
      241 accttgtgat gagtggcgac gagtttatgg aaaggttctc ctggcatgtg gcggataagg
      301 ggttccggat ggtgatacgg ggtcctgagt ccggtgaagc ggcgattact ggaagatgtg
      361 gtgtacgcca taacggagac agtgagaaaa acggcgaggc aaatcacaag gagagggatg
      421 tatcagcagt gactgaaggt tgacccgctg aagtgcgagc tgtgcggagg tcagatgcgg
      481 tttacggggc tgaagagggg ttaccgtctg gcagagcagg ttatgatgca tgagccgctg
      541 gctcgaatac ggtggtgcgg ctgagagtcg cagaggggaa gttgcgtcca tgttgagggg
      601 aacggagcaa aaaaccatca gagatacatt gaatcaatca atgtaagcaa tattaagtgt
      661 ctataccgat tacttgatga tcaggttgtt ttgaataaac gcgcattctt cggtgctttt
      721 ttaatctttt ggggatttaa attcctatcg atgaactgtc gatatgaaaa agcgagcatc
      781 atactgacgt caaacaaagg ggtcgcagac tggggagaaa tgttcggaga tcacgtgctg
      841 gcaacagcga tcctagaccg gctgctacac gctgaatatc aaaggagaga gttaccggtt
      901 aaaagagaaa cgtaaagctg gagtgctgac caaaaacaca acgccaatca gtgatgatga
      961 aatggtggaa aacggacagc atcagtaacg aaagtatctt agcaggcatg aaaatcgtcc
     1021 gcatccccta aacggtcaaa atgcttctgc cgtgaaggag ggagaatgcc atctcatcac
     1081 ttatatctgg cgaatgaaac acagatggtc ggccttgccc ccaaacacac ggcggtgcaa
     1141 aatggaatgg cagaaaactt cgtcaaaacg atgaaatatg attaaatcag tgttatgttc
     1201 gaacctgacg tgttaacagc agtaaagtag tctcgcgaag gtgttcaaac attataatga
     1261 atggcatcct catagtgcgc agggttatcg cttgctgcgg aaatatctga agcgacgtac
     1321 cagtaatggg gaaaatgata gctggtttct gaaaatatag aggaaaatgt gatatatata
     1381 tcctgttaat atttaaggag aatataaaat gaaagtctcg tttaagtctt taggctatat
     1441 ttttcatgat atatataata aaaaacacac tatcgatgag ttcaacgatg tggtaagaaa
     1501 agcagtttta tcaggtaaaa ttaacgagct gaatgcatgt cataaagtgg caatttttct
     1561 tgccgaaaaa gacaatgaga taacaaagaa agacaaagcc aagataatag atacattaac
     1621 tgaaaactat tccattgaat ttcaacaatt aatgaatatt agtgagcgca cgttaaattc
     1681 atctttatat attactcctg gtgaaagtgg ttttgtcagt tttgttaatc gtgaaggaaa
     1741 aatatgtcat actgcatatg taaaatcatc agataatagc atggcatatt accatgcaaa
     1801 ttatagcagc attgacaaat atattacgga tatgtgcggc cttatttgta tgaggcatat
     1861 tgagtctact ggcattattt tttatatgct tgatgaaaaa gttttatctg ccattgctga
     1921 atttatgaat gaaaaaggat ggcgggctgc gttttgttca gcaaagaact tatataaatg
     1981 tgtttaattt tagaaaaact atttggtaaa atccaacaag agtcgagagg ttcgcgttgc
     2041 ttcagcaggg cctcagcctg ttcaaccaga ctcagttgca ggtcgtgacg cagatttagt
     2101 ggtggtggtt gattgtcaga taccagccga tgatcttgta gtgcatatca tcagactttg
     2161 agaagcatat ggttttgcgg gtaagccgct taagatgtgt ccgcaggttc agattatgtc
     2221 gctctatccg ctgagtgcat ttcttgctta taatatgact ggttgaggac aataaagttc
     2281 ggtataccgg ctcaggcatc ggtcatgtag aaggcaatgc taaactggct cagcaaacta
     2341 agagcagcgc gcatccactt acccaatgcg gttgagaaaa tagccttcga tcgctttcat
     2401 gtggcgaaac aaccgggtga ggtggtggat aaaacccgcc agaatgaaca tccgcatctt
     2461 cctgttgaaa gtcgtcgtca g
//
LOCUS       DADLSE010000286         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLSE010000286 DADLSE010000000
VERSION     DADLSE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.289"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..565
                     /locus_tag="JN692_004230"
     CDS             <1..565
                     /locus_tag="JN692_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7266328.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="JN692_004231"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="JN692_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7266329.1"
                     /translation="MEHSRIKKRNVALIEKCIMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN692_004232"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="JN692_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7266330.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="JN692_004233"
     CDS             2167..2403
                     /locus_tag="JN692_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7266331.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          536 c          625 g          650 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcatcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccggtttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagata
//
LOCUS       DADLSE010000287         2435 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLSE010000287 DADLSE010000000
VERSION     DADLSE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.290"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..310
                     /locus_tag="JN692_004234"
                     /pseudo
     CDS             1..310
                     /locus_tag="JN692_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005108985.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            336..644
                     /locus_tag="JN692_004235"
     CDS             336..644
                     /locus_tag="JN692_004235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266332.1"
                     /translation="MNVEKEIYTGLYLFGNMKLNTSCGGVSAVIKVPTYNSNVLFFIN
                     DSHVSEEDSMENAKCYKFNLEELESIQKAIERALNDIRNLESLSRRNLLKDKAYRNPI
                     "
     gene            815..895
                     /locus_tag="JN692_004236"
     CDS             815..895
                     /locus_tag="JN692_004236"
                     /inference="COORDINATES: protein motif:HMM:NF015490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266333.1"
                     /translation="MWYYYTDLTGTVQEVTASDGTLVWAG"
     gene            883..1779
                     /locus_tag="JN692_004237"
     CDS             883..1779
                     /locus_tag="JN692_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HAY7266334.1"
                     /translation="MGWLTSRTGQMYDHDHYYYDKAGNLLTDEYQSAVMDNRLPGYGR
                     DRYRYNEWGELTERRDQQLEWNAQGQLTRVISSNSDTRYQYDALGRCISKATYGRHTD
                     RGERSRTTFVWEGFRLLQETTWQGKRTYLYDAEQPYTPVAAITGRGESQKIWYYHTDL
                     TGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSGAYFEQPQRLPGQYFDEETGLHYNL
                     FRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLGWIDPLGLNSLGIDSNNIFRGDGNY
                     KGGGIGQPKSGTISAQDIIDHL"
     gene            1879..2082
                     /locus_tag="JN692_004238"
     CDS             1879..2082
                     /locus_tag="JN692_004238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266335.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            2100..2420
                     /locus_tag="JN692_004239"
     CDS             2100..2420
                     /locus_tag="JN692_004239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266336.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
BASE COUNT          766 a          436 c          659 g          574 t
ORIGIN      
        1 ttatggttta ctcaaaacac aatatcagac gtgtttacac aaggaaaatg ggccgggaga
       61 tcggttgaag aagcgattca tgaggctagt gtcacggggc ggcttcctga tgggctgagt
      121 attagttatg aaaagataat tctgccaagc ggggaggagg tttgggcgac tctaaataat
      181 cgtacgcttt atgtggccca acaggcaaat gttacaatta acccagtaga taaaaatgga
      241 aaaggattaa atcagttgaa taaacttttg gatggaaggg cacccactcc agaacaaccg
      301 gatgttttaa taaaacactg ctaacaggat gcgttatgaa tgtagaaaaa gaaatttata
      361 cagggcttta tttgtttgga aatatgaaat tgaacacatc atgcggaggt gtatctgcag
      421 ttatcaaagt accgacatat aatagtaatg ttttattttt tataaatgat tcacatgtaa
      481 gtgaagaaga tagcatggaa aatgcgaaat gttataaatt taacttagaa gaattagaaa
      541 gtatacaaaa ggcgattgaa agagctttaa atgacatacg aaatctagag tctttatcac
      601 gcaggaattt gcttaaggat aaagcttata gaaaccccat ataatccagc actctgaagt
      661 aatgcgggcg cacaacttgc cctcatatag acgcagggaa tacaagctgt tgctggaaat
      721 accatagcag agcgagcgca ggatgtatct ttacgactcg gagcaggcgt acaccccggt
      781 ggcgagcgtg acggggcgct gagaaagccg aaaaatctgg tattactaca cagacctgac
      841 aggcacggtg caggaggtga ccgcgtcgga cgggacgctg gtgtgggctg gctgaccagc
      901 cgcacgggtc agatgtacga ccatgaccat tattattacg acaaagcggg caacctgctg
      961 acggacgagt atcagagcgc ggtgatggat aaccgcctgc cgggctacgg gcgtgaccgt
     1021 taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcaact ggagtggaac
     1081 gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg atacgcggta tcagtatgat
     1141 gcgctgggca ggtgcatcag caaggcgaca tatggccgcc atacggaccg gggagaacga
     1201 agccggacga cgtttgtgtg ggaagggttc cggctgttgc aggagacaac gtggcaggga
     1261 aagcggacat acctgtacga cgcggaacag ccgtacacgc cggtggccgc catcacgggg
     1321 cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac ggtacacgag
     1381 gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac
     1441 cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct gccggggcag
     1501 tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc gccggagtgc
     1561 gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt atatgcttat
     1621 gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg aatagattca
     1681 aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca gccaaagagt
     1741 ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga tgggcgttta
     1801 acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga ttttttctca
     1861 aaaaaccatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa aaataatcag
     1921 atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa
     1981 attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga ggtgttagtt
     2041 catgggcaaa ttcttggaaa taaagttagc cgatgtaatt agttagtgga gaggaagttg
     2101 tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc catgtgctaa
     2161 aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat tgtttagatt
     2221 gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg agtccagaag
     2281 agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga gctaatttga
     2341 atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat aaaaacggca
     2401 atgaaataga gtctgactaa attttattga tatag
//
LOCUS       DADLSE010000288         2422 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLSE010000288 DADLSE010000000
VERSION     DADLSE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.291"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..420
                     /locus_tag="JN692_004240"
     CDS             <1..420
                     /locus_tag="JN692_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7266337.1"
                     /translation="LTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLA
                     ITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEI
                     GFSSGFYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            463..966
                     /gene="yeaK"
                     /locus_tag="JN692_004241"
     CDS             463..966
                     /gene="yeaK"
                     /locus_tag="JN692_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7266338.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(967..1071)
                     /locus_tag="JN692_004242"
     CDS             complement(967..1071)
                     /locus_tag="JN692_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266339.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            1241..1687
                     /locus_tag="JN692_004243"
     CDS             1241..1687
                     /locus_tag="JN692_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7266340.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPITSGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1644..>2422)
                     /locus_tag="JN692_004244"
     CDS             complement(1644..>2422)
                     /locus_tag="JN692_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7266341.1"
                     /translation="HEAAVAFCIHAGTDELTSPVHQHRKGQLILALHGAITCTEENAL
                     WMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTCCTLKISPLCRELILT
                     LANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKIRTMVEMMAKGPVEWG
                     SLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQGLVKGDTVQKVAHTLG
                     YDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
BASE COUNT          598 a          597 c          591 g          636 t
ORIGIN      
        1 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
       61 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
      121 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
      181 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
      241 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
      301 gagatcggct tctcttccgg tttttatgcg atgaaagaaa acgatacgtt acatgatgcg
      361 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
      421 taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa
      481 ggaagcgtga cgcatcagca gttaatcgct ttattatcac aagaaggtgc taacttccgc
      541 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
      601 ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
      661 gttctggcaa tcctcgcagc cgatcaacag gccgatctga gccaactcgc cagccatata
      721 ggcggattac gcgcttcgtt ggccagcccg gcggaagttg atgaactcac aggctgtgtc
      781 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta
      841 ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt
      901 ttgaaaaccg cggattattt gcgcattgcg caaccagaac tggtcaattt ccgccgcact
      961 gcgtagctag cctgtccgtt caataagcag aacggacaaa accagcacaa ccgcaatagc
     1021 aaaaaacgat gatgtgataa tcagtgtctc tacgaacatt tgatcgttca tagcatgccc
     1081 ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt
     1141 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
     1201 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     1261 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     1321 aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     1381 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcac
     1441 cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt
     1501 ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga cgttaatggg
     1561 cagccagccg caactggttg ctgggctgct ggtgggcact gttttaggcg tagcgctgtt
     1621 tcgcggcgta ccggttggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     1681 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     1741 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
     1801 cctttcacta atccctgaag cgccataatc agctgtaact gctgacgcca ctggcgaaaa
     1861 ctcaaccccg tctctttcac tatcagtcgg gcaagattgc gttcactcat cgcaaaaaag
     1921 ccagcccatt gccccaatga cccccactcg acgggccctt tcgccatcat ctccaccatc
     1981 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
     2041 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     2101 gtcctgtttg ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa
     2161 catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
     2221 gctgtcactt gattgctatg cttcaccccg cccggtatcc aaaccgcata ttgtggcggc
     2281 accatccaca aagcattttc ctccgtacag gtaattgcac catgtaatgc tagtatcagc
     2341 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     2401 aatgcgaccg cggcttcatg gt
//
LOCUS       DADLSE010000289         2410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLSE010000289 DADLSE010000000
VERSION     DADLSE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.292"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN692_004245"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN692_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7266342.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN692_004246"
     CDS             1227..1760
                     /locus_tag="JN692_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7266343.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /locus_tag="JN692_004247"
     CDS             complement(1757..2074)
                     /locus_tag="JN692_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7266344.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN692_004248"
     CDS             complement(2250..2396)
                     /gene="ecnB"
                     /locus_tag="JN692_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7266345.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          614 a          634 c          597 g          565 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct accggtaacg
     1921 atttcatcgc ccaggcaagt agcgccatac tgactatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acggctaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggaaat ttggggccgt
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta cctttatgac gatcgcagaa aaaacaaacg
     2221 gcacgacact gctgtcgtac cgttattgct tattgctgcg ctttcgttgc tgcgccagaa
     2281 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
     2341 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
     2401 tcttcctttt
//
LOCUS       DADLSE010000290         2385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLSE010000290 DADLSE010000000
VERSION     DADLSE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.293"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            329..2385
                     /gene="tssH"
                     /locus_tag="JN692_004249"
                     /pseudo
     CDS             329..2385
                     /gene="tssH"
                     /locus_tag="JN692_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459269.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
BASE COUNT          565 a          565 c          662 g          593 t
ORIGIN      
        1 gtatcactgt gtagagtgtt tctttcaaaa gaataagttt tctaaatttt tagataagca
       61 atgctgatca atttacttta tcaatataag ttttcgcgga aatatatatg gcgattttta
      121 atttaatata ttcatggtgt gtatgaaatt ggcttactct attgaatatt accatgaaaa
      181 agatactacc tgctatatta cattgtgttc tgtgatatgc atcattgttt cattctgcct
      241 ttttgataat tctattaatt agaagtttta atcggtacag ttatgtttat gtatcattta
      301 tatgttatta catcatcagg tgtaacagat ggacactcct gtttcgcgca gtgcactata
      361 tggaaagcta accaaatctt tgtttagatc tttggaatct gcgacaacgt tctgtaaatt
      421 acgcgctaat ccttatgtcg aactgacgca ttggttgcat cagttaatac aacaaccaga
      481 gaatgacatt cacaatgtgc ttcgacatta tcaaataaca tctgctgatg ttgagaaggc
      541 gttaattcag caattgaata tattgcctgc cggagcgagt gtaattaacg atttttccca
      601 tcatatcgat ctttctgttg aaaaagcctg gatgttggca agtatccgct ttggcgatca
      661 aaaaattcgt agtggatggt tattacttgc ctggttaacc acgccagaat tgtgtcgggt
      721 gttaaaaaat atttgtgcgc cattggcaga gctaccggta gatgaactat tagatattct
      781 tccgtcttta atcacgtcat ctcctgaatt aacagaaatg ccttatgacg gctcggattt
      841 aaattctgca gtaccggggg aagctagcca ggtctttgcc ggcgaatcaa ctggaagcaa
      901 atccgcgctg gcgaaatact gtcaggacat gactgcgctg gcccgtgaag gcaatatcga
      961 tccggtcacc ggtcgcgaac acgagatccg tactatgacg gatattctgc tgcgccgtcg
     1021 ccagaacaac ccgctactga ccggcgaagc gggagtcggg aaaacggcag tggtggaagg
     1081 attcgcgctg gcgattgccg ggggggaagt accgccttca ctgcgtaacg tgcggttgct
     1141 gtcactggat gttggcgcgc tgctggctgg ggcgtccatg aagggtgaat ttgaatcccg
     1201 cctgaaagcg ttgctggaag aggccgcgca ctcgccgcag ccggtaatcc tttttgttga
     1261 tgaagttcac acgctggtgg tacatccggt acgggggatg ctgccaacct gctgaaaccg
     1321 gcgctggcgc gtggcaccct gcgcaccatc ggcgcaacca cctggagcga gtacaaacgc
     1381 catatcgaaa aggatccggc gctgactcgg cgttttcagg tattgcaggt tgctgagccg
     1441 gaagaggttg cggctgttga gatggtacgc gggttagtca ccacgcttga aaaacaccat
     1501 gacgtgctga tccttgatga ggccgtgcgc gccgccgtac agctttctca ccgttacatt
     1561 ccggcccggc aactacccga taaggccatc agcctgctgg atacggcggc ggcccgtgta
     1621 gcgctgtcgc tgcatacccc gcctgcgaga gtccagtatc tgcgccagca gctcaacgct
     1681 gccgaactgg agctggagtt gttattaaag caggaaaaaa tgggtatccg ctcggagcgg
     1741 ggcgatgtac tggcggcgcg gattatatcg ctgaatgccg atctttctga caccgaagcg
     1801 cggtggcagc aggaactgga actggttcac cgattacagg aacagcgtgc cgcagaaccc
     1861 ggcgagcgtg acgaaatcac gctgcaacag gcagaaacgg cattaagaga ctggcagggc
     1921 gaaaaccccg tcgtgttccc ggaagtcagc gcggcagtgg tggccgcaat tgtttctgac
     1981 tggaccggta ttcccgccgg acgcatggtg aaagatgaag ccagtcagat cctggaactg
     2041 cccgcccgtc tggcgcagca tgttaccggg cagagggacg cgctggcgca gattggcgag
     2101 cgtatccaga ccgcgcgggc gggtttaggt gaccagcgta agcctgtggg cgtctttttg
     2161 ctggccgggc cgtccggtgt tggcaagact gagaccgctc tggcgctggc agaggctatc
     2221 tatggcggtg agcagaacct gatcaccatc aacatgagcg agtttcagga gtcccacacc
     2281 gtttccacgc tgaaaggcgc accgcccggc tacgtgggtt atggcgaagg cggcgtgttg
     2341 acagaagcgg tacgccgcca tccctggagc gtggtattgc tggat
//
LOCUS       DADLSE010000291         2313 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLSE010000291 DADLSE010000000
VERSION     DADLSE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.294"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..517
                     /locus_tag="JN692_004250"
     CDS             <1..517
                     /locus_tag="JN692_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7266346.1"
                     /translation="VDNIDGKSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENKES
                     LFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRSDH
                     SLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYG
                     GSSGTETRPLQ"
     gene            546..>2313
                     /locus_tag="JN692_004251"
     CDS             546..>2313
                     /locus_tag="JN692_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7266347.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVLGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          691 a          544 c          503 g          575 t
ORIGIN      
        1 ggtagataat atagatggta aaagccgtgc cccatttatt ataaccccac cgctatttcg
       61 tcttgaggct ggcgatgact catcactgcg aattattaaa acagctgata acctgcctga
      121 aaataaagag tcgctgttct acattaatgt tcgtgccatt ccagcaaaga aaaaatcaga
      181 tgatgttaat gctaacgagt tgacgctggt atttaaaaca cggatcaaaa tgttttatcg
      241 ccccgcacac ctgaagggac gggtaaacga tgcgtggaaa tcactggaat ttaaacgtag
      301 tgaccattca ctcaatatat ataacccaac tgaatattac gtcgtatttg ccggactggc
      361 agtcgataaa accgatctca caagcaaaat tgaatatatc gcgcccggag aacataaaca
      421 gttaccactt ccagcatctg gcggaaagaa cgtgaaatgg gctgcgatca acgattatgg
      481 cggcagttcc gggacagaaa ctcgtccact gcaataaaaa atataaaaac acaggtcatc
      541 agggaatgcc tcaacgacac caccagggac ataaacgcac accgaaacag ttggcgctca
      601 ttatcaaacg ttgtttgccg atggtgctca ctggcagcgg catgctttgc actaccgcta
      661 acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa
      721 caaccgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact tatcaggttg
      781 atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc gccaatgcag
      841 agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt attaaggtgg
      901 atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa
      961 tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat ttgagcattc
     1021 cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt tgggacgatg
     1081 gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt taccgccagg
     1141 gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt ggcccctggc
     1201 gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg aacactatca
     1261 gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg ggagaaagcg
     1321 ccaccagcgg cagtattttt tccagctaca cctttactgg cgtgcaactc gcttccgacg
     1381 ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtactcggt atcgcaaaca
     1441 gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag
     1501 cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat ttagaagtca
     1561 cgattgaaga aagtgacggt acacaacgtc gctttatcca gccttattct tcattaccca
     1621 tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg
     1681 caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat ggtttgaata
     1741 atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg ctggggatcg
     1801 gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac agagctgaca
     1861 cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtacgcag tacatcaaag
     1921 atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc aacgatggct
     1981 attttagttt tgatgaagcc aatacccgca attggggcta taacagtcgc caaaaaagtg
     2041 aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat gcctccggtt
     2101 cacagcaaga ctattggggc aataacgaga aaaacagtaa tatctctgtt ggggtttccg
     2161 gccagcaatg gggaattggt tacagcctga attatcaata cagccgctac actgatgaaa
     2221 ataatgaccg tgcactctct ttgaatctca gtattccgtt agaacgctgg tggatttgcc
     2281 cctatatttc cagacatctg ttatcactta acc
//
LOCUS       DADLSE010000292         2258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLSE010000292 DADLSE010000000
VERSION     DADLSE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.295"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN692_004252"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN692_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7266348.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..>2258)
                     /locus_tag="JN692_004253"
     CDS             complement(1803..>2258)
                     /locus_tag="JN692_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAY7266349.1"
                     /translation="SAHWPLQGSNDKGNNHSCVVKVDDGTNSILLTGDIEAPAEQKML
                     SRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKVALASASRYNAWRLPSNKVKHRYQ
                     QKGYQWLDTPHQGQVTVNFSAQGWRISSLREQILPRWYHQWFGVPVDNG"
BASE COUNT          585 a          590 c          533 g          550 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagtatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgct
//
LOCUS       DADLSE010000293         2248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLSE010000293 DADLSE010000000
VERSION     DADLSE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.296"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..881
                     /locus_tag="JN692_004254"
     CDS             <1..881
                     /locus_tag="JN692_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7266350.1"
                     /translation="GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWL
                     TLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLT
                     VMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDID
                     HFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKAL
                     AERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETG
                     RNKVVVRDVVNFCESP"
     gene            979..1977
                     /locus_tag="JN692_004255"
                     /pseudo
     CDS             979..1977
                     /locus_tag="JN692_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2012..>2248)
                     /locus_tag="JN692_004256"
     CDS             complement(2012..>2248)
                     /locus_tag="JN692_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266351.1"
                     /translation="RHNLRGSMSAKGCCYDNACVESFFHSLKVECIHGEHFISREIMR
                     ATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT          662 a          421 c          481 g          684 t
ORIGIN      
        1 gggggccgat agttgctcac atactttcta gtcattatga gagctacaat ttacacattg
       61 ccgagttaac caatgaaaat ggtcaggtgg tttggaaagc ctcatatgtt accataatga
      121 ttttcatgtg gttaaccttg ctttcggtta atctatactt taatggttta cgctatgata
      181 tctggaatgg agtgacggtg attgcttttt gtgccgtact gtacaatatc tctctgttat
      241 ttatgagcag gtatagcgtg tccacttggt atattagtcg cactattgaa gtagtcagta
      301 agttaaccgt tatggtaata tttatgtgcc atatattcag tgcgctacga gtaacaaaga
      361 acattgcaca tcgcgatccc ttaaccaata tatttaacag aaattatttt tttaatgaac
      421 tgacagttca atcagcatca gccaaaaaaa cgccttattg tgtcatgatt atggatatcg
      481 accacttcaa aaaagtcaac gacacctggg ggcatccggt tggcgatcag gtgataaaaa
      541 cagtggtgaa tatcattggg aaaagcatac gaccagatga ccttttagcg cgcgtcggcg
      601 gcgaagagtt tggcgtcttg ctgacggaca tcgatactga acgtgcgaaa gctttagcgg
      661 aaaggattcg ggaaaatgtt gagcgtttaa ctggcgataa tcctgaatac gctataccac
      721 aaaaagtgac gattagtatt ggcgctgttg tgactcagga aaatgcgtta aacccgaatg
      781 agatctatcg actggctgat aacgcactat acgaggcgaa agagaccggg cgtaataagg
      841 tggttgtgag ggatgtggtg aatttttgtg agtcaccata aagcggcatt ttagcgggtg
      901 attttggtaa gcgctaaata gctattcgca gacttgcgta acaattttca gaaatatcat
      961 agtactatat gatcttaaat gcttatcatt gatcaatctg ctatcacccg cttttactgg
     1021 caattgtact ataatttctt aggttgcatc accctgatgc gtggggaaac atgtatggta
     1081 ttcaacaaaa aaatgtttgt actcattata ataccaggca ttctgggggt gttactatcg
     1141 tttgctatgt caatatttca aatgaataga gatacgacaa ttaccgccgg tattctgtta
     1201 aaacagcttg ataatgttac gcaaattgtc cagcatacga caaagcttac ctctattctg
     1261 gtgatgaagc cctgcaaaga cattcttgaa cagttgattg ctaacggagc attgactccc
     1321 tatgttcgaa caacgggtct tatagaaaat aatttccaaa tatgttcttc agttagcggt
     1381 ttcaaaaaaa tgaatgttaa cgatgtttac ggtacgagct ttcacaataa aaataaagaa
     1441 tcacgtatag tatcaattag cggtacaagt tttgtgccag gtaaaacagc aatagtgttc
     1501 cttatgccaa tagggaatga tatgacagct tttagtattg tagagtcacg atatatctat
     1561 gatctaatgg atgttcttga tgatgagaat gatgattcat tttctcttcg ttttactgaa
     1621 gggcctgcaa ttattagcgg tgtaaataat aatgacaggt tatatatgtt aaaaagagat
     1681 tttaattctg cgatcagtca ggctcgtctt acagttacta cgccgatgat atcgttatat
     1741 ccctatatca ttagcaatgt gctttatatt ttgccgttat ctattattct ctcttttatt
     1801 ctctatttct tatggcagca ctggatgtca cggaaaatgt cgctggcaga agaaattaaa
     1861 aaaggaatgt catccggaga attttctgtg cattatcaac cagtgtgcga cacgacaacc
     1921 aaagcgtgtc tgggggttga agcgttaatg cgctggcaac gcgaagatgg taaaaatata
     1981 ttgatcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2041 ccggactgag gccgccacac caactgtgcc gccgccaccg attgtaatca cattcgatat
     2101 aattaaacac cgttgcccgc attatttccc ggctgataaa gtgttctcca tggatacatt
     2161 ccactttcag cgaatgaaag aagctttcca cgcaggcatt atcgtagcag caaccttttg
     2221 cgctcatact tccacgcaga ttatgccg
//
LOCUS       DADLSE010000294         2245 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLSE010000294 DADLSE010000000
VERSION     DADLSE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.297"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            11..565
                     /locus_tag="JN692_004257"
     CDS             11..565
                     /locus_tag="JN692_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7266352.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            667..1158
                     /locus_tag="JN692_004258"
     CDS             667..1158
                     /locus_tag="JN692_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7266353.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1222..1997)
                     /locus_tag="JN692_004259"
                     /pseudo
     CDS             complement(1222..1997)
                     /locus_tag="JN692_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(2024..>2245)
                     /locus_tag="JN692_004260"
     CDS             complement(2024..>2245)
                     /locus_tag="JN692_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="nucleator protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HAY7266354.1"
                     /translation="SALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDG
                     QLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
BASE COUNT          522 a          531 c          591 g          601 t
ORIGIN      
        1 aaatatatta atgaacgagt tttctatcct ctgtcgtgtg ctgggttcgc tctattaccg
       61 ccaaccgcaa gatcctttac tggtgccgct gtttactctg attcgtgagg ggaaactggc
      121 tgcgaactgg ccactggagc aggatgagtt actgacgcgt ttacagaaaa gctgtgatat
      181 gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg ccgtgccgcc
      241 atatcgtagc gcatgggttg aggacgcaac ggaagcggaa gtgcgcgctt ttctttccga
      301 acgagggatg ccactggcgg atacgccagc cgatcacatc ggcacattgc tgcttgcggc
      361 ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa cactgttcag
      421 tgagtatctg ttaccctggt gtggtgcgtt cctcggcaaa gtggaggccc atgcaaccac
      481 gcctttctgg cgcaccatgg caccgctaac ccgtgatgcc attagtgcaa tgtgggacga
      541 gctggaagaa gattctgaag agtaaatgtg atcatcgtca ctcataactg caacagataa
      601 tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc tgctatcatg
      661 cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc cggtatctgg
      721 ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctggg ctgtacggcc
      781 tactttgctt gcccgcaagg tgggctgaaa gggctggcga tctccgctgc aaccctgctt
      841 agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc acatctggaa
      901 attctcggtt atgtcataac cggtattgtc gcgtttctga tgtgtattca ggccaaacag
      961 ctgctgcttt catttgttcc ggggacgttt ataggcgcat gcgcgacgtt tgccgggcag
     1021 ggtgactgga aactggtgtt accttcgctg gcgcttgggc tggtatttgg ttacgcaatg
     1081 aaaaacagcg gcctgtggct ggcggcgcgt agtgcaaaga cggctcaccg tgagcaggaa
     1141 atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aacagtaaat gccggattat
     1201 taattccggc atttttatct gtcaggattc cggtggaacc gacatatggc ggtatttcac
     1261 cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac ggtcgatacc
     1321 atcattaatc aggaaaatga cccctgtttc gatagccgac atcagacaca gcataacggg
     1381 ttcgtttgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa taaagcggaa
     1441 aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg aggagaggat
     1501 ctcaccggta ctcacattga cgacacgcag gttcacggca atctgatcga gctggtattg
     1561 cgtgtctgca ccgatcccaa aatatcttgc cccaacaccg cccgatttaa cgttgctttc
     1621 ataaccgata atcgaacctt caaccataat atttgccgcc gttaaagatt gcagcgggat
     1681 tcggttatta atggcaaccg tgccgttttc ttgtgccgca cgaataatct tgcgctcgtt
     1741 aagcaggttt tgtaagccct ggcgctttgc ggaacagcag tggagaagtt acttgccggg
     1801 tagggtttaa attgcctggt ttcgtcctga atgttgtata ccgaaacaaa gatcttaccc
     1861 gtcggcgctg gcagatgggt caaatctttg tagctctgag cacgaggcat taatgtcggt
     1921 ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac ggcaaccaaa
     1981 agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa tcggttgagt
     2041 tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga tctgtcacgt
     2101 tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg gtcaccatgc
     2161 ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt gactggatgg
     2221 cctgagtaaa gttatctaac gctga
//
LOCUS       DADLSE010000295         2227 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLSE010000295 DADLSE010000000
VERSION     DADLSE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.298"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1026
                     /locus_tag="JN692_004261"
     CDS             <1..1026
                     /locus_tag="JN692_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HAY7266355.1"
                     /translation="LLPFAHELRKRGVNNRIGFFLHIPFPTPEIFNALPTYDTLLEQL
                     CDYDLLGFQTENDRLAFLDCLSNLTRVTTRRAKSHTAWGKAFRTEVYPIGIEPKEIAK
                     QAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLAYEALLEKYPQHHGKIRY
                     TQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRY
                     SDVGLVTPLRDGMNLVAKEYVAAQGPANPGVLVLSQFAGAANELTSALIVNPYDRDEV
                     AAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKV
                     ATFPKLA"
     gene            complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN692_004262"
     CDS             complement(1033..1461)
                     /gene="uspC"
                     /locus_tag="JN692_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HAY7266356.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FNLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          624 a          479 c          486 g          638 t
ORIGIN      
        1 ctgttgccct ttgcgcatga attacgcaaa cgtggggtga ataatcgcat tggttttttc
       61 ttgcatattc ctttcccgac accggaaatt ttcaacgcgc tgccgacata tgacaccttg
      121 cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga tcgtctggcg
      181 ttcctggatt gcctttctaa cctgacccgc gtcacgacac gtcgcgcaaa aagccataca
      241 gcctggggaa aagcatttcg aacagaagtc tacccgatcg gcattgaacc gaaagaaata
      301 gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc ggaattgaaa
      361 aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt gccggagcgt
      421 tttctcgcct atgaggcatt gctggaaaaa tatccgcagc atcatggtaa aattcgttat
      481 acccagattg caccaacgtc gcgtggtgat gtgcaagcct accaggatat tcgtcatcag
      541 ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg gacgccgctt
      601 tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg ctactctgac
      661 gtgggcttag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga gtatgttgct
      721 gctcagggcc cagccaaccc gggcgttctt gttctttcgc aatttgcggg agcggcaaac
      781 gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc agctgcgctg
      841 gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga aatgctggac
      901 gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga cctaaagcag
      961 atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt tccaaagctt
     1021 gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct cacttgaggc
     1081 aatcactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt gattaccgca
     1141 aatcaccaaa ttgaaatgat gtttgtgaca tacttccaga atatgttcgc ttaactcacc
     1201 gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact tatcaagaaa
     1261 gctttgcgtt tcttcttgca tcacgctgcg taaatcttca agcatcggcg cggctaattg
     1321 attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct ttactggcct
     1381 ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg caacagcgac
     1441 aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg tgacattaaa
     1501 gctttactcc taatggagaa acgacgcaat cccaactcag tcaaacaggt aaatattagt
     1561 ctgattatta gtaaagacaa ttaatcaatc actctcggtg atcatatgat ttcaactgta
     1621 attataagaa aaaaatgtac tgattccccg cattaaacaa gtggctaata tatgaaaaac
     1681 attttattaa cattaagttg attgttgcct ttctttgtat ttaattagtt tgttgtgcgg
     1741 taagtgtctg tttaaaaata gcttatctgg tattgcatga aagtgattat ttatagcaga
     1801 tgattattta cggtgagtta ttttaaccgt gcgcaacatc ccatttcgat tattcctgtt
     1861 tcatttttgc ttgctagcgt agcgaaaaac tttttaacag attgaaatac acccaaaaca
     1921 aaagtatgac ttatacattt atgttaagta attgagtgtt ttgtgtgatc tgcatcacac
     1981 attattgaaa atcgcagccc ccctccgttg tatgtgcgtg tagtgacgag tacagttgcg
     2041 tcgatttagg aaaaatctta gataagtgta aagaccccct tctatttgta aggacttatt
     2101 aaaccaaaaa ggtggctctg cttattgcag cttatcgcaa ctattctaat gctaattatt
     2161 ttttaccgga gcttcccggc gacatcacgg ggtgcggtga gaccgcataa aaataaagtt
     2221 ggttatt
//
LOCUS       DADLSE010000296         2222 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLSE010000296 DADLSE010000000
VERSION     DADLSE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.299"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            125..1098
                     /locus_tag="JN692_004263"
                     /pseudo
     CDS             125..1098
                     /locus_tag="JN692_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1095..2186)
                     /locus_tag="JN692_004264"
     CDS             complement(1095..2186)
                     /locus_tag="JN692_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7266357.1"
                     /translation="MFKIFKIPVNKWTISTAALGGIFIVSGLILLMNYNHPYTFKAQK
                     AVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQARVDRLMADIVTAEHKQ
                     RALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFSERDIDVARQNYLAQE
                     ASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLEQTIVRAPSDGYVTQV
                     LIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGDDAEVVFNALPGKVFS
                     GKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHTDLSALPDGIYAQVAV
                     YSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
BASE COUNT          584 a          558 c          515 g          565 t
ORIGIN      
        1 tgtccggtat tccattttaa aataagataa aaaagagtcg gcataataat gcttacaatt
       61 tctccggtgc aagattcata tttatatagt aatatataaa attatatata attgggctgt
      121 tgcgatgaca aaattacaac ttaagtaccg ggagttaaaa attatctcgg taatcgctgc
      181 cagtgaaaat atcagccatg ccggactgta cttggcatcg cacaggccaa cgtcagcaaa
      241 tatcttgctg attttgaatc aaaagtgggt ttaaaagtct ttgaccgaac cacccggcag
      301 ctgatgctca caccttttgg caccgcgctg ttaccgtaca tcaacgacat gctggacaga
      361 aacgagcaac ttaataattt tattgcagat tataagcatg agaaacgtgg tcgggtcacc
      421 atctacgccc caaccggcat aattacctat ttatccaaac atgtaatcga taaaattaaa
      481 gatatcggtg atatcacctt atcgctaaaa acctgcaatc ttgagcgtaa tgccttttat
      541 gaaggcgtcg aatttcctga tgattgtgat gttttaatca gctacgctcc accaaaagat
      601 gaatcgctgg ttgccagttt tattacacaa tatgccgtaa ccgcttatgc cagccagcgc
      661 tatcttgaga aacatcctat tagccgtcct gacgaactcg aacatcactc ctgtattttg
      721 atcgactcga tgatgatcga cgacgcgaat atctggcgct tcaacgtcgc cgggagtaaa
      781 gaggtgcggg attatcgcgt gaagggaaat tacgtttgcg ataacacgca atcggcgctg
      841 gagctggcac gaaatcacct ggggattgtg tttgcgccag acaaaagtgt gcagagcgat
      901 ctgcaagacg gcacgctggt gccctgcttt cagcacccgt atgaatggtg gctggatctg
      961 gtggctatct tccgtaaacg ggaatacctg ccctggcggg tccagtatgt gctggatgaa
     1021 atgctgcgtg aaattcgcca ccagcttgct cagtcgcagc aactgcggcc tgaacaagcg
     1081 gcggaaagcg aagattaatg atcgagataa aggtaatgca cccagctggt catgcgtaat
     1141 agcactttgc gcatcaccga gacatggctg aaatgatcag agtagaccgc cacctgggcg
     1201 taaataccgt ctggtaaagc gctcaaatca gtgtgctcat ccagttcaat ggtcgcgata
     1261 acgccatctg aacccggcgc tgtgtttaac gtctgtaagg tgccggtcga ctgataagct
     1321 ccgccgggaa cagctggact aatggctgcc agcttgccgc tgaatacctt acctggcaga
     1381 gcattaaaca ccacttccgc atcgtcgcca ggggccagtc gcagcaagga gttctgacgg
     1441 aactgcgcca caatttgtcg tttctgatcg ggtataaaca ccatcaccgg acgtagcggc
     1501 agcgacgcgg cataggtacc cggacgaatc agcacctggg tcacataacc atcgctcggc
     1561 gcacgcacta tcgtctgctc aaggttatat tttgcttccg cgagctgcgc tttcaggctg
     1621 gcgatttgag aatgttcacc caacaccagg ctatccagct ggctctggat ctgtttttgt
     1681 tccgccgccg atgactttac ggaggcttcc tgcgccagat aattttgccg cgccacatca
     1741 atatcgcgtt ctgaaaacgg gtttactttc gcctggctgc cgcgtgcgta acgctgatac
     1801 tctttagcga atttatcccg cgtggccttt gcctgctgag tattcgccgc catctcatct
     1861 aactctgcgc ccaacgcccg ctgtttatgt tctgcggtaa cgatatccgc catcagccga
     1921 tccacccgcg cctgataacg cgtcgggtcc agtcgaaata gcacctcacc ttttttaatc
     1981 agcgtatttt tcttatccgt cacttcgatc accacgcctg tcacctgtgg gacaacagga
     2041 atagaaataa ccgctttttg cgctttgaag gtatacggat ggttatagtt cattaacaga
     2101 attaaaccac tgacaataaa tatgccaccc agggccgctg tggaaatggt ccatttattt
     2161 acaggaattt tgaagatctt aaacattgcc catgcaaaag ccacataggt caaaataatc
     2221 ag
//
LOCUS       DADLSE010000297         2168 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLSE010000297 DADLSE010000000
VERSION     DADLSE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.300"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            207..1922
                     /locus_tag="JN692_004265"
     CDS             207..1922
                     /locus_tag="JN692_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAY7266358.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            1919..>2168
                     /locus_tag="JN692_004266"
     CDS             1919..>2168
                     /locus_tag="JN692_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7266359.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNIST"
BASE COUNT          461 a          558 c          563 g          586 t
ORIGIN      
        1 tttcgttttt tacatctgaa ctttgcaatt ttccattcat tttaatatcc ctccggcgaa
       61 ttatgctcac aacctgaact taacagaaaa caatccgttc agggatacga cgtttgtatt
      121 ttaagaatct gactgcctga cccgacgcat tttacctctc cctatattca tgcgtccggg
      181 acataaacat aaataagggc tatgagatga attcgttaca aatcttgagt tttgtcggtt
      241 ttacgctgct ggtggcggtg atcacctggt ggaaggttcg caaaacagat accggatcgc
      301 aacaaggcta ttttcttgcc ggacgttcac taaaagcgcc ggttattgcc gcttcgttaa
      361 tgttaaccaa cctttccacg gaacaactgg ttggtctttc cgggcaggcc tacaaaagcg
      421 gcatgtcggt gatgggctgg gaagtgactt cagcggtgac gctgatcttc ctcgcgctaa
      481 tctttttacc gcgctatctg aagcgcggca ttgccaccat ccccgatttt ctggaggaac
      541 gttatgataa aacgacgcgt attatcatcg acttctgctt cctcattgcc accggcgtct
      601 gctttctgcc gattgttctc tactccggcg cgttggcgct caacagcctg tttcacgtcg
      661 gggaatcgct acagatttct cacggtgcgg ctatctggct actggtaatt ttgcttggtc
      721 tggcgggaat tttgtatgcg gtgatcggcg gactgcgcgc aatggcagtg gcggactcca
      781 tcaacggtat tgggctggtt atcggcgggt tgatggtgcc ggtatttggc ctgatcgcga
      841 tgggcaaggg cagctttatg cagggcattg agcaaattac caccgttcac gccgagaaat
      901 taaactcagt cggtggcccg accgatccct tgccgattgg cgcggcattt accggtttaa
      961 ttctggtgaa caccttttac tggtgtacaa atcagggcat cgtgcaacgc acgctggcgt
     1021 caaaaagcct ggcggaaggg caaaaggggg cgctgttaac ggcggtgctg aaaatgctcg
     1081 acccgctggt actggtgctg ccagggttga ttgcgtttca tctgtatcag gatctaccga
     1141 aagccgacat ggcctacccg acgctggtca ataacgttct gccagtgcca ctggtgggtt
     1201 tcttcggcgc ggtgttattt ggtgcggtga tcagtacctt caacggcttt ctgaatagcg
     1261 ccagtacatt attcagtatg ggtatttacc gtcgcatcat taaccagaat gccgagccgc
     1321 agcagctggt caccgtcggg cgcaaatttg gtttctttat cgctatcgtt tcggttctgg
     1381 tagcgccgtg gatcgccaac gcgccgcagg ggctgtatag ctggatgaaa cagctcaacg
     1441 gcatttacaa cgtgccgctg gttaccatca tcattatggg ctttttcttc ccgcgcatcc
     1501 cggcgctggc ggcaaaagtg gcgatgggga ttggcataat cagctacatc accatcaact
     1561 atctggtgaa gttcgacttc catttcctct atgtgctggc ctgtacgttc tgcatcaacg
     1621 tggtcgtgat gctggtgatc ggttttatca aaccgcgcgc cacgccgttc accttcaaag
     1681 atgcgtttgc ggtggacatg aaaccgtgga aaaacgtcaa gatcgcgtca attggtatcc
     1741 tgttcgcgat gattggcgtc tatgtcgggc tggctgaatt cggcggctac ggtacgcgct
     1801 ggttagcgat gatcagttat ttcatcgctg ccgtagtgat tgtctacctg atttttgaca
     1861 gctggcggca tcgtcacgac ccagccgtaa cctttactcc cgacgcgaag gatagcctat
     1921 gaaacgcccc aattttctgt tcatcatgac cgatacccag gccaccaata tggtcggttg
     1981 ctatagcggt aaaccgctga atacgcaaaa tattgatagt ctggcggcgg aaggtattcg
     2041 ctttaattcc gcctacactt gttcaccggt ttgtacgcct gcacgcgccg gactattcac
     2101 cggtatctac gctaaccagt ccggcccgtg gaccaacaac gtcgcgccag gcaaaaacat
     2161 ctccacta
//
LOCUS       DADLSE010000298         2106 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLSE010000298 DADLSE010000000
VERSION     DADLSE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.301"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..814
                     /locus_tag="JN692_004267"
                     /pseudo
     CDS             1..814
                     /locus_tag="JN692_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1253..2090
                     /locus_tag="JN692_004268"
                     /pseudo
     CDS             1253..2090
                     /locus_tag="JN692_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          523 a          511 c          632 g          440 t
ORIGIN      
        1 ccagtacacc tcagttctgg gccttgcccg tagcagttat ttttatcacc gggctgcact
       61 gaaagccggt gataaatacg ccacgatacg taagatgctg acagatatct ttaacagtaa
      121 ttaccagtgt tatggctatc gtcacctgca tgcgatgctc aggcatgagg gggggcggct
      181 atcagaaaag gttgtacgca gacttatggt ggaggaacag cttgttgtca gtcgtaaccg
      241 tcgtcgtcgc tacagctcat attgcggaga aatcggaccg gctccggata accttatcgc
      301 cagagatttt aaggcggagc aacctaatca gaaatagctg acagatatca cggagttcca
      361 gttccctgca ggtaaagtct ggctgtcacc agtggtggac tgcttcgatg gaaaagttgt
      421 gagctggtct ctcagtacac gccctgatgc tgaactggtc aacactatgc tggatagcgc
      481 tgtcgaaacg ttaaatgctg gcaaacgacc ggtgatacac agtgacagag gtgggcatta
      541 tcgctggcca ggctggctgg aaagagtgaa tgcagcaggt cttattcgct caatgtcccg
      601 taaaggatgt tcacctgata atgctgcatg cgaaggcttt ttcggcagac tgaaaacgga
      661 aatgtattat gggcgtaaat ggtcgggcat cacgccagaa aagttcatgc agcaagtgga
      721 tgcttacatc agatggtata acgagcggcg tataaaatta tcgctgggtg ccgtcagccc
      781 caaaatgtac cgccaacaat gcgggctgga atgataaagc agtccaggaa atcgtccgca
      841 tccccccttc accatttgga ttgtccgctc cacccaacat gttgtttcct taaggttcct
      901 acaccacaaa ggaaactctc atgctgcgca gtggatggct gacgttgccg acaggcggga
      961 acttcgccag ttcaggcaga caccggaaca gcgcttcacg caggaacagg agcatctgca
     1021 gccgctgctg ggtacggact tcgatatccg tcatgtctcc tgggatggct atatcgaggt
     1081 tggcgggaac cgttacagtg tgccggaaag cctgtgtggt cagctggtat caatacggat
     1141 ctcgctggat gatgaactgc ggatctacag taatgagcaa caggtagcat cgcaccgtct
     1201 ctgttcggct gcatacggct ggcagaccgt gccggagcat cacgctccac tctggcagca
     1261 ggcttcggag cgcatggccg aacggctggg ggagatacag aaacgggtaa tcactgtctg
     1321 tgaccgggaa gcggatatct ggcattacct gtattataaa gtcagccacg gacagcgtgg
     1381 tgcgtgctgc acagaatcgc cggctggaag aggcacccgg caagctcttc gaactgccgg
     1441 aagtcctggc aaccgcagga agccacacgc tgaatgtgat gcaaaaaggc gggcgggcag
     1501 cccgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat cccgacaaca
     1561 gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag gacggtgcct
     1621 gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga cgtattgtca
     1681 gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa agtggtggta
     1741 catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg ttatccaggc
     1801 gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag aaacacaaaa
     1861 tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg ttaagctgga
     1921 aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg atggcatgac
     1981 agcaaacgca caggtcgtcc cggttggggc gtcatgtggg atggctggtc agacttcagg
     2041 atatggttga gggttacctg gtgatgaagt ctcttgatca ggagctctga tcaagagaca
     2101 gcagct
//
LOCUS       DADLSE010000299         2091 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLSE010000299 DADLSE010000000
VERSION     DADLSE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.302"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>2091
                     /gene="ehaA"
                     /locus_tag="JN692_004269"
     CDS             <1..>2091
                     /gene="ehaA"
                     /locus_tag="JN692_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HAY7266360.1"
                     /translation="VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNN
                     GGEVNAREHISLGYYDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAG
                     IIDAEKIEFVWASTSDKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNS
                     AFSGKVKIEQNGALGITQNIGTAEINNCGKLHLKADDSMTFANKISGNGTISIDSGTV
                     ELTGNNYAFSGYIDVASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGRG
                     IVEINMGNHEFSFDEFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSLVTVG
                     KGAHSISTLGFSGGTVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVS
                     RDIDSALSLTEVDDGNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNG
                     QKAAVGTYDYRLTSGVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKI
                     TGSGDLAFSSQKGQTVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETEL
                     DMNGHSQTVGTRNGSADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDN
                     SNLTANVNIANSANVLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTN
                     NGTLMTGMSGQQAG"
BASE COUNT          565 a          443 c          597 g          486 t
ORIGIN      
        1 tgttaagggc accagtcgtc tgacttatac gcaggggaat gtaagcggcg aaggtagtca
       61 ggtaaatgcc gggaccttct ttatgggcgt ttacggcgga tatggcggta atcagttcct
      121 gtcggttaat aacggtggtg aagttaatgc tcgtgaacat attagcctgg gttattacga
      181 ccaacgttct gatacaacat tagtcgtatc agatggaggt aaaatatccg caccgaaaat
      241 tagtttaagc accaactctg agttagcgtt aggtgcacag gaaggaagtg ctgcgaaggc
      301 ggcagggatt attgatgccg aaaaaattga gtttgtgtgg gcttcgacat ccgataagaa
      361 aatcacctta aaccacacgg ataaagacgc gactatttcc gcggatattg tcagtggcag
      421 cgagggcctg ggctatatca atgcgctcaa tggcacgact tacttaaccg gtgataactc
      481 tgcctttagt ggtaaagtca aaattgagca aaatggcgct ttagggatca cccaaaatat
      541 aggtacagca gagatcaaca actgcgggaa attacacctg aaggctgacg atagcatgac
      601 ctttgccaat aagatttccg gcaacggtac aataagtatc gacagtggga cggtggagtt
      661 gaccggcaat aactatgcct tcagcggata tattgatgtt gcttctggtg ctgtcgcggt
      721 tatttctgaa gataagaaca tcggccgtgc agagctggat gttgatggca aattgcaaat
      781 taatgccaac aaagattggg tatttgataa cgatcttgaa ggtagaggca ttgttgaaat
      841 aaacatgggg aatcacgaat tctccttcga tgagtttgct tatacagact ggttccaggg
      901 ttcactggcg ttccagaaca cgacatttaa tctggaaaag aatgctgagt ttctgcagaa
      961 aggcgggatc actgcgggtc agggaagcct ggtaacagtg ggtaagggcg ctcactccat
     1021 tagcactttg ggattctccg gcggaaccgt tgattttggt gccctgacag caggtgcaca
     1081 gatgacagaa gggacggtca acgttagtaa aacgctggat ttgcgcggcg agggtgtgat
     1141 tcaggtttct gacagtgacg ttgtccgctc agtatctcgt gatattgact ctgcgttatc
     1201 gctcactgaa gtcgatgatg gtaacagcgc cattaagctg gttgatgcgc aaggtgcgga
     1261 agttctgggc gatgcgggca atctgcaatt gcaggataaa aatgggcaaa tcctctccag
     1321 cagtgcccaa cgtgatattc agcagaatgg gcaaaaagcg gccgtcggca cttacgacta
     1381 tcgtctgacg agtggggtaa acaatgacgg tctgtatatc ggttacggcc tgacccagct
     1441 tgatttacac gctaccgaca gcgatgctct ggtgctgagc tctaacggta aaagcgagaa
     1501 tgccgccgat ctcagcgcaa agattaccgg cagtggtgac ctggcattca gcagccagaa
     1561 gggtcagacc gtatcgcttt ctaacaaaga caacgactat accggtgtta ccgatctgcg
     1621 cagtgggacg cttttgttga ataacgataa cgtgttgggt aatacccatg aactgcgtct
     1681 ggcggcagag actgaactgg atatgaatgg tcacagccag accgtgggca cgcgcaatgg
     1741 cagcgccgat tcactgctga gcttaaatgg cggcagtctg acggttacca acgggggcac
     1801 ttcaaccggt tcgttaacgg ggagcggaga gctgaatatt cagggcggca cgctggacat
     1861 cgcgggcgat aacagcaacc tgacggcgaa tgtgaacatt gctaattcgg ctaatgtcct
     1921 ggtaagtcat gcgcagggat tgggtagcgc aaacgttgag aacaacggta ccctggcgtt
     1981 gaataatagc gctgaaaaaa gagcggctgc gtctgtgaat tacaccctgg gcggcaatct
     2041 gaccaacaac ggtacgctga tgaccggaat gtcaggacag caagctggca a
//
LOCUS       DADLSE010000300         2071 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLSE010000300 DADLSE010000000
VERSION     DADLSE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.303"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            7..2071
                     /locus_tag="JN692_004270"
                     /pseudo
     CDS             7..2071
                     /locus_tag="JN692_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          777 a          363 c          380 g          551 t
ORIGIN      
        1 tacatcatgt acaaatcatt atttactatt atcattctac tgtatagcgc cagttcatat
       61 gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat cgatcatgtt
      121 gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga tcgtttttat
      181 ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga atcaatggat
      241 attactgaca aagcctatgc cgacaaatta aaaaaattag aaactccttt tcttgatgat
      301 cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat gctgacagac
      361 ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat gtctactgta
      421 tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt tattcaatct
      481 cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta tatctcatca
      541 actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga cacagataac
      601 ttctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg aaagataaaa
      661 aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag ttattacaat
      721 agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa cggtgagttt
      781 attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa cgacaaaatt
      841 aaagatggtg aatgccctca tttttatgaa tgatggaaaa atcaaacagc aacatagcta
      901 tctgaatagt aaactcgatg gtcctgcata tgaatattat cctgatggca aactcatgca
      961 ggaaaatttc tatcagtaaa gtgtgttgat tggaaacgat accagttact accaatccgg
     1021 aaaaataagc tctatacaca atagaaattc ccgtggtcag tatgatggag ctaacgaacg
     1081 ttatagtgaa gagggtaagt tactgtctaa atccgtctat aaagacggaa aacaaatttc
     1141 tgttcaaacc tggtatgaaa atgtgcagaa agaagaggag aaacattttg atgagcaagg
     1201 ccaattaaat ggtctggtta agcaatggta taaaaatggt aatcttgcaa aatcacaaaa
     1261 ctataaacat gacatactcg atggtaacag tgaagagtgg tacgaaaatg gcatccctga
     1321 aagtttgtac ccttataaga atggaaaaac cgacggcatt gccaaatcat ggaataagta
     1381 tggtaaatta acctattcaa tcgaatacaa aaatggcgta gagaatgggg catatcgtaa
     1441 ctggagcaaa aacacaggta aactaaccaa agaaaccctg tacgtcaatg gtatcagaca
     1501 gggagtggaa aaagagttta acgatcgcac aggtaaacta ttaacctcca cgcaatatgt
     1561 taataataaa agacatggta cagaggaaac ttatgatcaa aacggaataa aatatattac
     1621 atgctatcaa aatgatcaga aattatcgtc tttagacaat ccaacacaaa taaaagataa
     1681 tgcaacaaca ggcgattcca gtgctcaatt tgcattaggg aaatacgaat tcatctgtgc
     1741 aaatatagat gaagggatta aatggttaac caaatctgca gagcaaaaaa acactgatgc
     1801 gatatacttt ttagctacag cctacaaagg caatggtata cctgcaaaca atgaaaaata
     1861 cctggcatat ctgcaacaag ctgcaacact gggcagtagt aatgcccagg cggaaattgg
     1921 ctatttatat ctcatcggaa aagaactacc acagaatctt cctgatgctg gtgtctggtt
     1981 caaaaaagca gctgcacaag gtaattttgc cgcacatttc tatctgggaa ggatgtatca
     2041 gaatggtgac ggcgttgaga gaaatatgga a
//
LOCUS       DADLSE010000301         2041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLSE010000301 DADLSE010000000
VERSION     DADLSE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.304"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(218..382)
                     /locus_tag="JN692_004271"
     CDS             complement(218..382)
                     /locus_tag="JN692_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266361.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1284..1568
                     /locus_tag="JN692_004272"
     CDS             1284..1568
                     /locus_tag="JN692_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7266362.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1556..2041
                     /locus_tag="JN692_004273"
     CDS             1556..2041
                     /locus_tag="JN692_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7266363.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          526 a          425 c          585 g          505 t
ORIGIN      
        1 aaaatgatgg acattatttt tgtggaaccg gaggtaacag accagacggt ttaaatgagc
       61 cggttacttt aaacgggtga tggacagcac ctcaaaagtt cagatggatt atatttgtct
      121 gcaatacccc gggtattttc gatttgcgaa atggatggaa cgacttgctc aggggatagc
      181 tgatggagtt actgaagtac cgaaggacca ctaaaaatca caggccggga atttcggcgt
      241 ttccggcgta ggtgtgccac gaatgcatcc agtggcgtga tgttggatgc aatgctgtat
      301 gagagatgag gggcggcgtc cagacgcagc cttcaaaccg ccttgagctg ctgccgttaa
      361 gagcggtggt ggtgagcgtc aaggttaaaa ggcgcagaac atgtgtcagg taggtgttgc
      421 ggagatgaac gaaagtgaac tgtcgatgaa gtgtcgaaag gaacctctag gcgatgtcga
      481 aaatgtgcta tctacgcagc gcattacgag tccagcagat atctggtact ggctgggcgg
      541 catccggcat ataggcagca tgaacgcaac acaggctctc gtacggaacg tgggaacctg
      601 tcgtctcgat gctaagggag cggcgcggag cagaggcact gcaagcgcct gagtaccgat
      661 gcgagacaca ggggcagatg gcttcgtagt agtgaggaag tgcctgtaat gggcatggag
      721 cgaagggggt cagtcgttgg ggctggttta tcggtcaacc ggatagccgg gaggagccca
      781 tgagtcagcc aaagccgttc gaggtctcta agtatgctgt ttgaaaagca taccagcggg
      841 tcaaagctaa tcgaggtgcg gcaggtgtcg atgggcagtc agtcgaggcg tttgaggtta
      901 ccaatcgaag ctatacccgg taatggcgca ggttgacctg cacttggcca agtggattgc
      961 ccgcaaacac aagcgagcgc gaggtagtct ggtgcgtgca ttcgagtggt tactgcgaat
     1021 tcgtcacgac tgcccgacgc tgtttgcaca ctggtgctta gcttacgata cttgaatggt
     1081 tcaacggcaa gagccgtatg acgtgagagt gtcacgtacg gttccgtggg agcgtagggg
     1141 ggcggtgccc ctgcgccacc cgatatcccc tttgagtgcg gttacgtcca cagaggtaaa
     1201 tgccgctgac gcggcatctg taatctgtta cgttgtgtat atcatttgct atatattgtc
     1261 aatatgctat tgttgtaaag caaatgatat acggaggctt tatgaaatca ggtgttcagc
     1321 tcaatctcag agcaagagaa tcacagcgga tactcattga tgctgctgca gaaattcttc
     1381 ataagtcgcg tacagatttc attctggaaa tggcctgtaa agctgctgaa gatgttattc
     1441 tcgaccggcg ggtgtttaat tttaatgaca ggcaatatga agagttcatt gaaatgcttg
     1501 atgctccggt tgcagatgat cctgccatcg aaaaattgct ggcaaggaaa cctcaatggg
     1561 atgtgtaact gcaccagaac ctttatcctc atttcatcag gtcgctgaat ttgtcagcgg
     1621 tgaagctgta cttgatgact ggttaaagca gaaggggcta aaaaatcagg caatcggagc
     1681 cacccggact tttgttgtat gcagaaaagg tacgcaacaa atagtcggtt tttactcact
     1741 ggccactggt agtgtcaatc atacagaagc tacaggtaac cttcggcgca atatgccaga
     1801 ccccattcct gtaattatac ttgcacgcct ggctgtagat gtgtcatttc gaggaaaagg
     1861 gcttggcgcg gatttacttc atgatgcagt tcgtcgttgt tacagggttg ccgaaaatat
     1921 cggtgtgcgt gcaattatgg ttcatgcgct tacggaaaac gcaaaacagt tttatattca
     1981 tcatggtttt aagccatcaa aaactcaggt gcaaacattg tttcttaaac tccctcaata
     2041 g
//
LOCUS       DADLSE010000302         2036 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLSE010000302 DADLSE010000000
VERSION     DADLSE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.305"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..683
                     /locus_tag="JN692_004274"
     CDS             <1..683
                     /locus_tag="JN692_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY7266364.1"
                     /translation="TMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAI
                     PNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLT
                     MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGYCWDNSPME
                     RFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKN
                     SNSVASFC"
     gene            677..1018
                     /locus_tag="JN692_004275"
     CDS             677..1018
                     /locus_tag="JN692_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7266365.1"
                     /translation="MLTTSVLNTLIHWVVFGVCIYAAHTSQALANFTGFVVAVSFSFF
                     ANARFTFKASTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAISLVCGFV
                     YSKFIVFRDAK"
     gene            1015..1944
                     /locus_tag="JN692_004276"
     CDS             1015..1944
                     /locus_tag="JN692_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7266366.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKRLPERNLFMKGILSWVGGQTDVVKYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
BASE COUNT          542 a          427 c          509 g          558 t
ORIGIN      
        1 ccacaatggc aacccggaga ggctaccaga tgggacgctg gcttgctggc aggctcatga
       61 aagagctggg gctggtcagc tgtcagcagc cgactcaccg gtataaacgt ggtggtcatg
      121 aacatgttgc tatccctaac taccttgaaa ggcagttcgc cgtgaccgag ccaaatcagg
      181 tgtggtgcgg tgatgtgacc tatatctgga cgggtaagcg ctgggcgtac ctcgccgttg
      241 ttctcgacct gttcgcaaga aaaccagtgg gctgggccat gtcgttctcg ccggacagca
      301 ggctcaccat gaaagcgctg gaaatggcat gggaaacccg tggtaagccc ggcggggtga
      361 tgttccacag cgatcagggc agtcattata cgagcaggca gttccggcag ttattgtggc
      421 gataccagat cagacagagt atgagccggc gcggatactg ctgggataac agcccaatgg
      481 aacgcttctt caggagtctg aagaacgaat ggatgccggt ggtgggttac gtaagcttca
      541 gcgaggcagc tcacgccata acggactata tcgttggata ttacagcgca ctaagaccgc
      601 acgaatataa cggtgggtta cccccaaacg aatcggaaaa tcgatactgg aaaaactcta
      661 actcggtagc cagtttttgt tgaccacttc agtgctgaac acacttatac actgggtggt
      721 ttttggtgta tgtatctatg ccgcacatac cagtcaggct ctggcaaact tcacaggttt
      781 cgtagtggct gtgagcttta gcttcttcgc gaatgcaaga ttcacattca aagcatcgac
      841 tacagcgatg cgctacatgt tatacgtggg atttatggga atattgagtg tgattgttgg
      901 ttgggcagct gataaatgct cacttcctcc aatagtcact cttatcacct tctccgccat
      961 cagtctggtg tgcggtttcg tctattcaaa gttcattgtc tttagggatg cgaaatgaaa
     1021 atatctcttg tcgttcctgt cttcaatgaa gaagaagcga tacctgtttt ctataaaacg
     1081 gtacgtgaat tccaagagtt gaagccatat gaagtagaaa ttgtattcat aaatgacgga
     1141 agtaaagatg ccacagagtc aattattaac gcgctggctg tttcagaccc gctagttgtt
     1201 ccgctgtcat ttacacgcaa ctttggtaaa gaaccagcct tatttgcagg gttagaccat
     1261 gcaagcggcg atgctgtaat tcctattgat gtcgacctgc aagacccaat tgaggttatc
     1321 cctcatctta ttgaaaagtg gcaggcaggt gctgacatgg tgcttgctaa acgttcagac
     1381 cgctcaactg atggacgact gaaacgtaag acagctgagt ggttctataa attacacaac
     1441 aaaataagca ccccaaagat cgaggaaaat gtcggagatt ttcgactcat gtctcgtgag
     1501 gttgtggaga acattaaacg gttgcctgag cgcaatcttt tcatgaaagg catactgagc
     1561 tgggtgggtg gtcagacaga tgtcgttaaa tatgtacgcg cagagcgtgt tgctggcatc
     1621 tcaaaattta atggctggaa attatggaat ctggcactag aaggtatcac aagtttttca
     1681 acctttcctc ttcgcgtatg gacttatata ggcttgtttg ttgcaagcat ttcattttta
     1741 tatggtgctt ggatgattat agacaccctt gtatttggta acccagtacg cgggtatccc
     1801 tccttgctcg tatcaatact tttcttgggt ggcgtgcaac tgatcgggat tggtgttctc
     1861 ggagaatata taggcagaat atatattgaa gtaaaaaata gacctaaata catcataaaa
     1921 aaatctcatc gaggtaaccc atgattaaaa ttaatttatt taaaaattca aatctccttg
     1981 ccttcatatc atgttttgca atatcaattt attgttattg ggggtggttg tacgat
//
LOCUS       DADLSE010000303         2026 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLSE010000303 DADLSE010000000
VERSION     DADLSE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.306"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..663)
                     /locus_tag="JN692_004277"
     CDS             complement(<1..663)
                     /locus_tag="JN692_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7266367.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSG"
     gene            complement(691..2022)
                     /locus_tag="JN692_004278"
     CDS             complement(691..2022)
                     /locus_tag="JN692_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7266368.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT          554 a          596 c          453 g          423 t
ORIGIN      
        1 accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga gaataaagcg
       61 gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg tcgttctgga
      121 aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct gggtaaacac
      181 cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga tggtgacaca
      241 cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat tgcaaggttc
      301 ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg caattgcctg
      361 cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta acgcccccag
      421 cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc ccagaccgtt
      481 cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt ccagttctgc
      541 cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca gcgccggttt
      601 tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa
      661 catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca gagaaacaca
      721 aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt tgtgcaatgc
      781 cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga aaatcgggct
      841 acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc aggccagcaa
      901 aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct cggcaggcat
      961 cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac
     1021 gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca ctttcaccca
     1081 cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc
     1141 gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg cgtagaaaaa
     1201 gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt tatgccgcgc
     1261 cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta acgtctggat
     1321 aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca ccagcagccc
     1381 cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg acacgcccag
     1441 cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga ataacaattt
     1501 ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat aaaagggact
     1561 gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac tatcgttggt
     1621 gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga gcgcacctaa
     1681 caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat ctttacattc
     1741 tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg ccgggtaacc
     1801 aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca ccgggagaaa
     1861 aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg tgtcagtact
     1921 gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac ccgccggggt
     1981 cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatt
//
LOCUS       DADLSE010000304         2020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLSE010000304 DADLSE010000000
VERSION     DADLSE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.307"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            278..1378
                     /gene="ompC"
                     /locus_tag="JN692_004279"
     CDS             278..1378
                     /gene="ompC"
                     /locus_tag="JN692_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7266369.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1542..>2020
                     /locus_tag="JN692_004280"
     CDS             1542..>2020
                     /locus_tag="JN692_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7266370.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSL"
BASE COUNT          629 a          402 c          391 g          598 t
ORIGIN      
        1 tattagcagt aattttgtgg ggatccctca ggtctacggc ccggcagtta atcagataaa
       61 ttccgacaat attaaaaaat aaatttcaaa taccagtaat tcaccttata tctcatttcg
      121 acttcgatca catatcgata aataatatta tttcatctat ttttctcatt gtttttaatc
      181 accattctat tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa attcgtttag
      241 aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa tagttgcggt
      301 ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct ataacaagga
      361 tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta ctgatgataa
      421 gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa cgcaaatcaa
      481 tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata acgatgaagc
      541 caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa ttagtgaatt
      601 tggctctctg gactatggtc gtaactacgg tgtcggctac gacattggtt catggaccga
      661 tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca tgacataccg
      721 taccaccggt ctagcaacct atcgcaacta cgatttcttt ggcttaattg aagggctgaa
      781 ctttgccgcg caatatcaag gcaaaaatga acgtactgac aacagtcatc tttatggtgc
      841 tgactacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg tttatgatgg
      901 ctttggtatc ggtgcggtat ataccaaatc cgatcggaca aatgcgcagg aaagagccgc
      961 tgctaatcct ctcaatgcct ccggtaagaa tgcagaacta tgggccacag gtataaagta
     1021 tgatgccaac aacatctact ttgcagctaa ttacgctgaa acattaaaca tgaccaccta
     1081 tggggatggt tatatctcta acaaagcaca aagttttgaa gtggtggcgc aatatcaatt
     1141 cgacttcggc ttgcgcccat cactcgctta cctgaaatcg aaaggcagag atctgggccg
     1201 ctacggcgac caggacatga ttgagtatat cgacgttggt gcgacgtatt tcttcaacaa
     1261 aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg actttacccg
     1321 tgccgtagat attcgcaccg ataacatcgt cgctacgggc attacctatc agttctaacg
     1381 tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc tgtatgactc
     1441 cttaagtaaa aatgagcaat ttttgatatt tctggtcatt cacctcaatt aatattattc
     1501 catatcctcc ggggacatct tactataata tttattgatg agtgattatg accaatatta
     1561 atacagcttg tgtaaaaaat aatgccagtt atcaacttaa taacgcatta cctaacaaag
     1621 aaaccatctc cagcaatttt tgtgaacgac tagaacaatg gggtaataaa tcggttaata
     1681 atggtgaaga aagagcaatt gccgtggagc gaattaaaga agcttacaat tcgaacatgg
     1741 catctcttga tttgtcatat cttgatttga gtgaattacc gcctatcccc tcgacagtta
     1801 atacgttaaa tctggaaaac aattgtctaa cttgtcttga cttagctgac aatgccagtc
     1861 tcgttaatat caacctcagc gttaataaaa ttaacacgat aacctttcca aaagaaccaa
     1921 aactggaaaa catttatatt gatcataaca atctggaaag tttagattta aaaaatcagc
     1981 attcattggt taatctggaa gcgcaaaata atagcctaaa
//
LOCUS       DADLSE010000305         1998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLSE010000305 DADLSE010000000
VERSION     DADLSE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.308"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN692_004281"
     CDS             <1..149
                     /locus_tag="JN692_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266371.1"
                     /translation="YREIDAIARKYVSLKYDGSIASDFSTMKCIDFIHGNELDELISR
                     SVKK"
     gene            263..748
                     /locus_tag="JN692_004282"
     CDS             263..748
                     /locus_tag="JN692_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HAY7266372.1"
                     /translation="MSYDIFLEIDGIDGESMDDKHKNEIEVLSWRWNIHQESTMHAGS
                     GLGSGKVSVTNLSFEHYIERASPNLFKYCASGKHIPKAVLTMRKAGGNPLEYLKYSFT
                     DLIVAMVSPSGSQGGEIASRESVELSFSTVKQEYVVQNQQGGSGGTITAGYDFKANKE
                     I"
     gene            816..1349
                     /gene="tssJ"
                     /locus_tag="JN692_004283"
     CDS             816..1349
                     /gene="tssJ"
                     /locus_tag="JN692_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HAY7266373.1"
                     /translation="MNKWRNPGGWLCAVALSFVCLLSGCSIDSDDVPDLMSQRLDLVI
                     KASDKVNPDNQKKAAPIEIRVYELKNDAAFTAADWWSLQDNDKAVLADDLLHRDSFIL
                     RPGEEKKLRHALNKETTTLGILAGYRNLGKSVWRATYKIPEPPEKSWYSRFMPKKKVE
                     LDAGIEQSAIVIKERDK"
     gene            1353..>1998
                     /locus_tag="JN692_004284"
     CDS             1353..>1998
                     /locus_tag="JN692_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HAY7266374.1"
                     /translation="MSWNNRVVWSEGQFLLPQMFQQQERYLEHVIHYRSLPLSPFFWG
                     FNEYGIGSEALNIGKLVLTKASGVFADGTPFNAPGHTPLPPPLTILPEHLNQLIYLAV
                     PIRTPNSEETSFDNNPESLARFSVHENEIRDANSLGRGAQLLQLSHLRLRLLPEKAVT
                     DAWIGLPLTRITALHPDGRVDIDKELIPPVANYQASTLICTWLSWINDLIKMRAD"
BASE COUNT          560 a          445 c          501 g          492 t
ORIGIN      
        1 tttatcgtga aatagatgcg atagcgagaa aatatgtatc attaaaatat gatggttcta
       61 tagcatcaga cttcagcact atgaaatgta tagatttcat tcatggaaat gaattggatg
      121 agttgatttc caggagtgtt aagaaatgaa atatattcaa tcctttgttc tgccgattcc
      181 atatgtggaa gagtgttgta aagtaatggt ttatttgtaa gcggataaac caaaattaat
      241 ttaaataaaa aaggtatgaa atatgtctta tgatattttt ctggaaattg acggcattga
      301 cggcgagtca atggatgaca aacataaaaa tgaaattgag gtgttgagct ggcgctggaa
      361 tatccaccag gagtccacca tgcacgcggg tagcggtctg ggatccggta aggtatccgt
      421 aactaatctt tcatttgaac actatatcga gcgcgccagc ccgaacctgt tcaaatactg
      481 cgcctccggc aaacacattc cgaaagccgt gctgaccatg cgcaaagcag gcggcaaccc
      541 actggaatac ctcaaataca gttttactga tttgatcgta gcaatggttt cgcccagcgg
      601 gagtcagggg ggagaaatag cttctcgtga gtcagtcgaa ctctctttca gcaccgtgaa
      661 gcaggaatac gtggtgcaga accagcaagg tggcagcggc ggcaccatca ccgcaggcta
      721 cgacttcaag gccaataaag aaatttaagc agtacttttc cggcccggca aatagccagg
      781 ccggttttta ttatttgatt ttaaaggaaa tcacagtgaa taaatggcgt aaccccggtg
      841 ggtggttatg tgcggtagct ttgtcttttg tctgcctgct ttccgggtgc agcattgaca
      901 gcgacgacgt gccggacctg atgtcacaaa gactcgatct ggtgattaag gcttccgata
      961 aggtgaatcc tgacaatcag aaaaaagccg cgcccataga gatacgcgtt tatgaactga
     1021 aaaatgacgc agctttcacg gcggcggact ggtggtccct tcaggacaat gacaaggctg
     1081 ttcttgctga tgatttactt caccgcgaca gctttatcct gcgtccgggc gaggagaaaa
     1141 aattgcgcca cgccctcaat aaggaaacta ccacgctggg gatcctcgcc ggataccgca
     1201 atctgggaaa atcggtctgg cgggctacat ataaaattcc tgagccaccg gaaaaaagtt
     1261 ggtacagccg ctttatgccg aagaaaaaag tcgaactgga tgctggcata gagcaaagcg
     1321 ccatagtgat taaagaacgg gataagtaaa ttatgagttg gaataaccgg gtggtctgga
     1381 gtgaaggaca atttttgctg ccacagatgt ttcagcagca ggaacgctat ctggaacatg
     1441 tcattcatta tcgcagtttg cctctttcac cttttttctg gggcttcaat gagtatggaa
     1501 ttggtagtga ggcgctgaac atcggaaaac tggtacttac aaaggcttcc ggggtatttg
     1561 ctgacggtac tccttttaat gcgccgggtc acacaccact cccaccgccg ctgaccatcc
     1621 ttccagaaca tcttaaccag ttgatttatc tggctgtgcc aatacgtacg ccgaacagtg
     1681 aagaaaccag cttcgacaat aaccctgaat cactggcacg cttttcggtg catgaaaatg
     1741 aaatccgtga tgccaattca ctggggcgcg gcgcgcaact gctgcaactt agccacctac
     1801 gcttgcgact attaccggaa aaggcggtga cagacgcgtg gatcggcctg ccgctgacgc
     1861 gcatcacggc gctgcatccc gatggtcggg tcgatataga caaagaattg atccctccag
     1921 tggcgaacta ccaggccagt acgctgatat gtacctggct atcctggatt aacgatctga
     1981 taaaaatgcg tgcagatt
//
LOCUS       DADLSE010000306         1980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLSE010000306 DADLSE010000000
VERSION     DADLSE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.309"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN692_004285"
     CDS             complement(131..1585)
                     /gene="ospC2"
                     /locus_tag="JN692_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7266375.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          628 a          340 c          315 g          697 t
ORIGIN      
        1 caggattccc ggggcgttca ccacatgcaa tgtgttgttg caggggaacg gtctgcacca
       61 tcccctatat cgatcaatat aacaaaaatg acaacgtcag attcagggca caacctaaat
      121 atattttagg tcacctccca aatcgtttgc ctgataccgc tccatttttt aataaaaaat
      181 caattgtttt tgaatctttg cttttcattg cattgtcaag catagtgtcc ccactatttg
      241 ctttttgaaa tcttttgttt acatcaacca atccattatt aataaaatat tctagcactt
      301 tatagttggc actatcttca ctaagtaaat actcaacatc atacagctca caatacatat
      361 tgttgttcat tttctccatc tctgcgacat cttgttttgt aaaattaaat ttagatagta
      421 aataaagtgc catatcagat tttgcattgc ctagagaata agcaagagct tgatgagcca
      481 tttttttatc agtcacctta ttgttaattt cttcataatt ggatgcttta atggcatcct
      541 ccaaagatat atctctgagt cttatctttt tgaagttatc tgtgcttagc attcttggta
      601 tatgatactc aagctgaaac acaaagttta taattctatc cagactaaca ggatcaatgt
      661 ttttattata acaaaactct cggaatcttt gatctttact ctttcttata aaatcaataa
      721 gatgcaaccc aattccaagc ctcatatctt tagtattaaa tattggcaca tcagtttttc
      781 cctgattacc atgcacatac ctgctaactt catccacaac ctctttaaag ttatctaaga
      841 aaaatgattg gtgctcatgg taaaaaacag gtggaatagc attatcaaag tggtcggtta
      901 atgtcatata tccatatgga gagtcatgat caatgatata cgcattagca ccaaaatcca
      961 cagtatgatg tgttttattt agtgggagtg tttcttgatg attggaaatt tcaaccccaa
     1021 aaaaaacaaa atcgtggtta gctatttctt caatatcctc agagaatgtg tttttatatg
     1081 tttgtatatt ctcagactta attttatcaa aggacttaat attaagagta ttatttgaca
     1141 ggttagcgtt actttgatgt ctgaagttaa aatcagaatt aataataata gagagcaagt
     1201 gacgttcatc cgaagaaaga ggatgagttt tttgtattat ttcagaaaga tagtgtctgg
     1261 acaccttatc gaggtgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa
     1321 tagaaagatt taatgatttg aaactggtcc cttgagagaa cattttacta taactttgcg
     1381 ctgctataga cttccttaaa aagcttttca cagtatcagc acagtgatct aaatgttttt
     1441 cacttatgcc agaagaggat gagtttgtta cgcatgatat gtttttttgt aatgcttttg
     1501 tatctttgtt cggatttatt tttccatcaa caagatcaat attgttctga acattaatat
     1561 gatttactgc ttcaggtatt ttcataaaac atcccccatt atgtgcgttt ttatctgcag
     1621 ttatttagaa taatattatc taacttaata ttttcttgta tgtattataa cgaacatatt
     1681 ttaatatatt gtacgatttt cactctgaaa tcccctgagt ttaatcacac ttcctttact
     1741 gaaaggaatt attgcaacag tattatttgt ttttggatat atatcttgca ccttgttatg
     1801 tagttaatgc tcagaatatg atagataaaa tagagacgca gaacagggca tgctcataac
     1861 atgatgataa cataaaaaat tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa
     1921 agagagctaa atagctgcgc ggaatagtag gctgtgtccc ttaatcatct gagttatagt
//
LOCUS       DADLSE010000307         1929 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLSE010000307 DADLSE010000000
VERSION     DADLSE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.310"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            463..1512
                     /locus_tag="JN692_004286"
     CDS             463..1512
                     /locus_tag="JN692_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7266376.1"
                     /translation="MYNNKIDSNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKFGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
BASE COUNT          626 a          326 c          356 g          621 t
ORIGIN      
        1 tctgcttttg ggaggcatgg gtcactcagg ccaaatgaca tccgacggtt tcacgatgaa
       61 ctacggcaaa agaatatcag agccctcatt cctccccgaa agggagcggg ctctggtctg
      121 gtgagtatgc agatcgtaaa ggctatggcc atggtgcgag agtggcagct atgccagaaa
      181 gagtgtgtat tgcctgagac tctaacctga tgcagagacg attgtcttga acctgattta
      241 ttcaacaaag ccgctggcag gatgttacga aagttatcgc ggccgcaggc tgatttgata
      301 tcgatactat cggtggtact gcggaagtaa atgcctatat agtgcgactt tgaaaaataa
      361 taaattaata ttgaactcgt tgattaataa tcataaactt tcaggcattc atatttgcaa
      421 tgagtgtttg aaaatcacac aaaactggag agcctacttt aaatgtataa taacaaaatc
      481 gacagcaaca gaaaaacaag gcatgattgg gttgatgctt taaaatttct cggtattttt
      541 gctatatatt taggtcacct tggtttgggg gctggcaaat tgtatccttt tgtttttagt
      601 tatcacgtcc cactattctt tttcgctgct ggttttttta ctatcaaaaa aaatgactta
      661 tctattttcg attatatcaa gtcaaagttt tacagattaa tgattccata tttcactttt
      721 gcatttacta tattaataat aaacacaatt aactcaggtg agacgattga ttatatatat
      781 agccacattt atgatataat atatggtgtt agaaacaacc aatttgtagg aacaatatgg
      841 tttataaact gcctttttgt tataatagca attgatgcaa tctttaaaga aatagtcaag
      901 aacaatatag tgattttaat catatcacta ttatcgttca tgttgtcaca aacagtgctc
      961 aatcataacc cattactaga tcctcaatgg ttttggaaca tagactccgc tatggcatac
     1021 tggtggttat tgcctctcgg tagatgtatg tttttagaat taacacgtga caggtttttt
     1081 ggaaaatcta aatttggttt cattgtcttc tcaattaccg cgataatgag tgcgtatcaa
     1141 ctccttaacc agaaaccttt gctttttaag attatctcaa tattcaatgc agacataata
     1201 tcaagtagtt atattcaagc tattaatact attatcacca cggtaggttt gataatattt
     1261 aatattttta tagcaaaaat aatttgtggg aatgacttta ttgtaagagc tggaagaaat
     1321 actcttaaca tatgtggaat ggagtatatc accaaattat ttatccctat ggctttagca
     1381 atcataggct tcagcgtgac cattccaaat cctatctgcg caatcatata tacctgtatt
     1441 tgtgtatatg tatcagataa aattggccat tggctatcga ggacagtcgg tgggccattt
     1501 ttgataaaat gataaaaagg cgcaattaat tgcgcctttt tatagatata tagcgcatat
     1561 ttatgctcag attatactgt taaaattaat gcgcaaatgt tacggttttc tttcttggca
     1621 actgaaggga atgttcgaag tgcaaacata aaattacccc ttcactgaac agatattcag
     1681 tcggccaaca catgagaaag aaatacacca gatgaattaa aatatcatat actcattaat
     1741 caaagaaata aaagcataaa cagtaggtgg tgacgctgat aaactggcta taaacaacca
     1801 ggaaggtgat gagctatcgc aatataattt attggtgaat tatttgaagc cgattgataa
     1861 caccagtgct caaaagactt cccccggtaa ccataagtag ctaatatcta gtacagtttc
     1921 gatatctat
//
LOCUS       DADLSE010000308         1927 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLSE010000308 DADLSE010000000
VERSION     DADLSE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.311"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN692_004287"
     CDS             complement(<1..1208)
                     /gene="pdeC"
                     /locus_tag="JN692_004287"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7266377.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVPVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDV"
     gene            1636..1917
                     /locus_tag="JN692_004288"
     CDS             1636..1917
                     /locus_tag="JN692_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266378.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          553 a          424 c          427 g          523 t
ORIGIN      
        1 tcgacatcaa gaaatgcatg ctcagtcact tcaaatttaa tttgctgtgg acgcaccgcg
       61 tattgctctg ttttctgatt gattcgcgct atcaaccgtg acgtatgaaa atcggaggcc
      121 gacaggttaa tagaaacata gcgatcggca tgtgttgcca ggtacgcgcc cagatcgcgg
      181 aagacattat caataacata atcagttatc tgttctatca tcccctcctt ttctgccagc
      241 ggaataaact ctgccggatt cattatttgc ccctgctcac caggccaacg taacaacgct
      301 tcagcgccga tacatttttc tgttttgata tcgattattg gctggtaata aagacaaagt
      361 tgatgttttt cgagggcgcg ttgcaattta cgtttgggag ataaatagtt ttgtcgaata
      421 cgtagccaga gtaatagcag aacaagactc cccaaaatac cggcgggcaa cgcaaaaata
      481 agatgattat aaaaatgggt tataagacgt tgatatgaag tcgcaacaat ggctgcaatt
      541 gggcgttttg tcgaataaac tgtcgcatat aaatagccat ttctttgtac ggttaaatcc
      601 ttcaaatgaa tcagcggaga aaacgttgct gccgaggcct ctttgcttaa cgagaaaaag
      661 gttttcgtca ccgtatcata cacaccccat tgcaatgtcg ggtcatcaga catcacttca
      721 ctccagaaga gagggttgat aaccggcaca taatttcccc gctgcatata ggtcatttta
      781 tagccagaga aaaaaggcgt atcgcggtaa taatagatag aaacgttagg ttcacgctta
      841 taatcggccg gtgcaatcgt atagccgttt acaggcgcta tcagcgatga gcataaaaaa
      901 tggttatcac gggcatagat caattcatta atataaagat agccacgaat aatattcaac
      961 attcgctttt gatgggctgg agtgcaaact tgcccctgat agcgttcagc tgcatcgctc
     1021 actaaatctg cctggcgaat gaccagctcg gatttatcca gtgccagttg agcaaaggtt
     1081 cggagctgat tattcacttc tgattttgcc caaaggaagg caatccatag cgaaagaatg
     1141 atgggaaaga gaactaaaaa gataatgccc ggcaacgcca gtaattggtg tcgtgcacga
     1201 tgactcattg ctatcaattt cctgcctgtt tatacccggc cattgcaagg tgaaaccctg
     1261 gctaccgacg tctttagata aatcttacgg tataaatagc acttttgcct gatttgaaat
     1321 tgataccttt ttaatcgtca cctagatgaa aataattcat tgatattcaa aattaatttt
     1381 ctacaggaat acagaggcgg cgggaagata tgcccttaat gccactgaaa aaccacataa
     1441 tctgtacagc aataaaagtc acggcctaat atggctaaca aacaatcaat tgtgatatag
     1501 ttcacaaaat taatgaaaca aacagagtgt ttcatttttg tgttctgcaa gcccgcgata
     1561 gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt
     1621 cagaggaatt gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcaactt
     1681 cttagtgccg taatgatgtt tctggccagc acactcaaca tccgttttcg tcggtctgat
     1741 tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg tttccgcctg ttggttcgca
     1801 atggggttgc ttggcatcac aatggcggac atcaccgcta tctggcacaa catcgagtcg
     1861 gtgatgatag aagagatgaa tcagacaccg ccacaatggc caatgatttt gacctgatac
     1921 gtagaag
//
LOCUS       DADLSE010000309         1920 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLSE010000309 DADLSE010000000
VERSION     DADLSE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.312"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..585
                     /locus_tag="JN692_004289"
                     /pseudo
     CDS             1..585
                     /locus_tag="JN692_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN692_004290"
     CDS             complement(626..1621)
                     /gene="pgl"
                     /locus_tag="JN692_004290"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7266379.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFSVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYSVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          494 a          513 c          453 g          460 t
ORIGIN      
        1 gtgcggattc tggaacaagg cacgaataat tgcatggaat cggtgctgtg taacgaatca
       61 gactttggca taaacatgaa caacgtcaca aattcatcca ttgattttac cccactggtc
      121 aacgagccat tcgtgctggc ctgtcggcgt gaccatccat tggccaaaaa gtagcttgta
      181 gaatggcagg agttagtggg ttacaaaatg attggcgtac gttcctccag cggcaaccga
      241 ttgttgatag aacaacagct agccgacaag ccctggaagc tggactggtt ttacgaagtg
      301 cgtcatcttt cgacgtcatt aggactagtg gaggcaggac tggggatttc ggcgcttcct
      361 ggtctggcaa tgccccatgc gccctattct tcgattattg gtatcccact ggtggaaccc
      421 gttatacgtc ggacattggg gattattcgc cgcaaagatg ccgtgctttc tccggcagct
      481 gaacgctttt ttgccttgct gattaatttg tggactgacg ataaagacaa tttgtggacc
      541 aatatcgtcg aacgccagag acatgcgtta caggagataa gttaatacac cggcgcagga
      601 gagactgcgc cggttaaatc agcggttagt gtgcgttaac caccacccac attggtccct
      661 gcccgaccgc atagcggcct ttttcatgca gtagcccctg ctcgccaaca atttcgtata
      721 ccgagatgtg gtgagatttt tgcccagcgg caatcagata cttgccgctg taatcaacat
      781 tgaagccgcg cggctgggtt tccgttggct ggaagccttc tttactcaac acgctgccat
      841 cttccgaaac gctgaaaacg gtaatcagac tggcggtacg gtcgcaggcg tataaatggc
      901 gaccatccgg ggtgatatga atatcagccg cccaacgggt gtcggagaag ttttccggca
      961 tcatatccag cgtctggaca cattcgatat tgccgtgcgg atctttcagt tcccagacat
     1021 ccactgagct gtttaactca ttgacgctat acgcatattg ttcgtttgga tggaatacca
     1081 tatgacgcgg gccggcccct tcaacggtag tcacttccgc agggtcctgc gccaccagat
     1141 gaccatcatc gctgaccgta aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca
     1201 gcgtacggtt gtccggtgag atattggcgg aatggcaacc gtccagcccc tcgaccacat
     1261 cgacgacgcc cactggcagg ccatcttcca gacgcgttac gctcacgtta cccgcattgt
     1321 aagaacctac aaagacaaac tgcccctggt gatcggtgga aatatgcgtc ggactacccg
     1381 gcagcacaga ctctgcggca aaggtcagtg cgccatcgtc cggggcaata cgatacgcca
     1441 ggacgctaaa ctcagggcga acaccaacat agagataacg tttgtccggg ctgaccacca
     1501 tcggctgcac ctgccccggc acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat
     1561 tcaggttcca gacgtgaatt tgctggctct cagggctggc gatataaact gtttgcttca
     1621 tgaacacacc tcaaaaatga taaatccctt atctttcata cgcataacaa cacttactgg
     1681 ataaaaaaca gacaacaaaa cacgataaaa tacaatatcg cacatcaatt aaatccataa
     1741 gttagcactc catttcgaat ggcatattac taaaatatta gtcaccgaca cgaatttgac
     1801 aatgccacag atattttcgc gactattcag ggagtatgac tctccccgct tttgtttacc
     1861 tgtatcaacg accgtaaaca ctatgccata acaacttctg gccaacttcc cattcgctca
//
LOCUS       DADLSE010000310         1847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLSE010000310 DADLSE010000000
VERSION     DADLSE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.314"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..804)
                     /gene="ipaH9.8"
                     /locus_tag="JN692_004291"
     CDS             complement(<1..804)
                     /gene="ipaH9.8"
                     /locus_tag="JN692_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7266380.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLADAVTAWFPENKQS"
     gene            complement(1066..1161)
                     /locus_tag="JN692_004292"
                     /pseudo
     CDS             complement(1066..1161)
                     /locus_tag="JN692_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1197..1502)
                     /gene="ccdB"
                     /locus_tag="JN692_004293"
     CDS             complement(1197..1502)
                     /gene="ccdB"
                     /locus_tag="JN692_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7266381.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1504..1722)
                     /gene="ccdA"
                     /locus_tag="JN692_004294"
     CDS             complement(1504..1722)
                     /gene="ccdA"
                     /locus_tag="JN692_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7266382.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          503 a          382 c          418 g          544 t
ORIGIN      
        1 agattgtttg ttttccggga accatgctgt cacggcatca gccagggggc gatggagtgt
       61 attctgttgt ccgtcactca tggagaagta aatctgcggg ccgtggtagt ccggcgaaga
      121 ggttaatctt tgcagggctt gcagagcatg ggatgataat gggttatcac taatatgtat
      181 tgaacattca ttcctcagat taagaatgct ttccgggata tgacttatct gatttctatc
      241 aaaaaaatac tctctgacaa cgggattatt tccctcagaa aatgctggta gctcagtgag
      301 gaaattacgg gtcgctctga gatttttaag agcctgtggg agcgatggta aggagactat
      361 ttcgttatag ctgatattca tagtcaataa cgaatcgggt aaagctggca ggttttccag
      421 ctcattgtgt tgtacctgaa gtgactccag cgcaggaggt agcacgggca attctgataa
      481 tttattgctg gcggaatata atttttttag cgtaacaggc agttcaggta ggttagttaa
      541 ttgattatat gatacattga gcagcgttat ctgagctggt aagttgtcag gtagcgagga
      601 cagatttaaa cggtccagtc gaagttcatc ggaattattg ataagacatt ctttaagtcg
      661 ggagacagcc tcatcacgct cttcaccggg gagcgcttgt ttttcccatt tatcccatgc
      721 tgaaaagtaa tcagcatatg taccggaaat agtgttataa aaagaatttt ggggcaatga
      781 aaagttatta tttatcggta acatgatggg catccagtta aagccggtgg acaaatggct
      841 ttgttaaaag aaataagtag gagtttttat gaagttcatt ccatgaaatg aacttcagca
      901 cttttttatt ataataagga taaacaaaat tggctgtaaa aaaacgatac tgtttcagtt
      961 aaccgtgaat ggcctgtttg caccgtattg ctcttgtttt tcggttagtt aaacatcgta
     1021 cttcatattt gaacggtaag cggctcgtca gaaccgtatt gatatttact gagaggtcag
     1081 atcaactttc cagggcaaca gatcgcgtac ccggtttgcc ggccagtcct ggatatgctc
     1141 aatgacgtaa cgcagccatt ttgtcaacgc cacgattttt gaccctagcc tgacatttat
     1201 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
     1261 gccacttctt ccccgataac gaagatcggc acactggcca tatcagtggt catcatgcgc
     1321 cagctttcat ccccgacatg caccaccggg taaagctcac gggagacttt atctgacagc
     1381 aggcgggcac tggccagggg gatcaccatc cgtcgtccgg gcgtgtcaat aatgtcactc
     1441 tgtacatcca caaacagacg ataacgactc tctcttttat aggcgtaaac cttaaactgc
     1501 atttcaccag tccctgttct catcggcaaa agagccgttc atttcaataa aacgggcgat
     1561 ctcagccatc ccctgctgat tttcagcctg ccagcgttcc gcacgcagac ggcgggcctc
     1621 tttttgcatg gccgtattta ccaggcctga gatattcacg tttgcggatt taagtaactg
     1681 atagctgtca ctgtctatag tgacagtaat acgctgcttc atgataagcc ctcaaaaaca
     1741 tacttatgat atgtatatga gtatatatta atggtacaaa ccatcaatat ccataaaaag
     1801 tacaccatta attcgctata agtttttaat acaatttttt ctgctgc
//
LOCUS       DADLSE010000311         1791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.315, whole genome shotgun sequence.
ACCESSION   DADLSE010000311 DADLSE010000000
VERSION     DADLSE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.315"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            461..1543
                     /locus_tag="JN692_004295"
     CDS             461..1543
                     /locus_tag="JN692_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HAY7266383.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
BASE COUNT          566 a          364 c          336 g          525 t
ORIGIN      
        1 acatgcccca tcacatcctc aacatcaaag tattagagcc ttgcaaacca ttatgcgtct
       61 cagtttgtct catctatctt acgcaaaaaa aatagaacta atatcaagcg gcgctatatt
      121 gcgtattaaa taaaaccacc tcaattacat taataaatct aaataaatat catgcaaata
      181 atttccatgg ttatttttag gctatttaaa ttaaaaaaat aaattatgag tcaatcataa
      241 ataacaacct ttaatataat ttgactacca aatttatatt cgttcacaaa atagtcatgc
      301 attgtgtgca atagaaacag ttcagataaa aatagggatc tacttcacaa tttaaatgag
      361 aaactaaaac ttacatcttg aaataatctc attgattgga tgaatatttg tagtgcagtg
      421 acatcatttt taaataatag ttcaaataaa agggctcacg atgaaaaaat taacagtggc
      481 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag cagagattta
      541 caataaagac agtaacaagc tggatctgta cggaaaagtt aatgctaagc actacttctc
      601 ttctaacgat gcagatgatg gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac
      661 tcaaatcaac gatcaactga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      721 tgctgaatct caaggttctt ccaaagataa aacccgtctt gccttcgctg gcctgaaatt
      781 cggtgactac ggctccatcg attatggccg taactacggt gtagcatacg atatcggtgc
      841 atggaccgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      901 gactggtcgc accacaggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      961 tggtctgaac tttgctgctc agtatcaggg taaaaatgac cgcactgacg taactgaagc
     1021 taacggtgat ggtttcggtt tctccactac ttatgagtat gaaggattcg gtgtaggtgc
     1081 aacctatgct aaatctgacc gcactaatga tcaggttatc tacggtaaca acagcctgaa
     1141 tgcatctggt caaaatgctg aagtatgggc agctggtctg aaatatgatg cgaacaacat
     1201 ctatctggct accacctatt ctgaaaccca gaacatgact gtttttggta ataaccatat
     1261 tgccaacaaa gcacaaaact tcgaagtagt tgcacaatat cagttcgact tcggtctgcg
     1321 tccgtccgtt gcttacctgc aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga
     1381 cctgattgaa tatattgatg taggtgcaac ctattacttc aacaaaaata tgtccacttt
     1441 tgttgattac aaaatcaacc tgattgataa gagcgatttc acgaaagcat ctggcgttgc
     1501 taccgatgat atcgttgctg taggattggt ttaccagttc taatctgatt actcaaagat
     1561 atgttgtggg aggctttgcc tccccaacat ataagtggcg ccctcaagcc actttcctta
     1621 gaagcactat cttgcttctg actatataaa ccttctgtta tatattaccc tttattttct
     1681 gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt atcaattaga
     1741 tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca t
//
LOCUS       DADLSE010000312         1774 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLSE010000312 DADLSE010000000
VERSION     DADLSE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.316"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..849)
                     /locus_tag="JN692_004296"
     CDS             complement(<1..849)
                     /locus_tag="JN692_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7266384.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIHSLTPFSLPMDLTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
     gene            complement(1238..1504)
                     /locus_tag="JN692_004297"
     CDS             complement(1238..1504)
                     /locus_tag="JN692_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266385.1"
                     /translation="MLTQTIFPCLPQKQENISLEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1613..1774)
                     /locus_tag="JN692_004298"
                     /pseudo
     CDS             complement(1613..1774)
                     /locus_tag="JN692_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          502 a          287 c          369 g          616 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattc gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc aagcttatct ggaaaggggg gaatgctgtc
      301 taatctgttt ccagaaacta ccagtaattt tagttccggt ggcaatagtg gcagaatatg
      361 caatcggtta ttatgaaccc gtaaatgagt tagattttca ggaagaaatg gtaattcttt
      421 tatcttgtta tcacccgcat taagctcttc taacgtctcg ggtaaggcag gtaagctttc
      481 cagttgatta aattgagcat gtattacctt aagcattggt ggtaaatcag ggagatgtgt
      541 taataggttt tttcttattt ccagtgttgt tatctgaggc ggtaaatctg gtaatgtttt
      601 taaatctaac tcagataaat ttaattctgc ctcttgattt tgtaaacata ttttcagcct
      661 attgaaggcg atattgcgtt gctcgcctgg ggtaccgttc ttttcccatt cactccaggc
      721 ttttaaataa aattcattac aagatggagt cagatccata ggtaaagaaa atggagttaa
      781 cgagtgtata ttgtttattt gatgcataat accagaaccg attgcctgta tattggttga
      841 tttaatcatt ataactagaa ttcttatcaa cgataagaat tctagtgaaa tagaatatag
      901 cttattaatt ttaaaacagt ttgctttgta ccctttctcc gggtattgct ttccccctaa
      961 gccgtctatg ggaaggagtc tgctgttatt cctgagaatg gtaattcagc cataaaaagg
     1021 tatttaaggt cattgcgtct ctccctgtac tcgaataatg gtgaagaaag catgatgaat
     1081 atatcacgat aacgttcagt acgtatcgag gataaatgga gttactcctc tctgaaatag
     1141 aattactgct ctattttttt ctgttttcag aagttattag taacaaaccc tgatatattt
     1201 ttttgaaaat ggagcttaaa cgagtctgaa attaacttca gaaatcagaa taccgttggc
     1261 tttgtttttc atcaattccg gcttcattta tctttgtcaa ccattgcatt tctgtagcag
     1321 tgaatttcaa cgttttcgtc cggttggcag caggtatttg taattcgtat atagctgctt
     1381 ttttattgaa aacagtatat ccatctgtag taacagtaga agataataat acaggatttg
     1441 atacttcaag gctgatattt tcttgttttt gtggaaggca agggaatata gtttgtgtaa
     1501 gcatattact tccttatgtt atattttggt aaaaaattta tttttttaca ttatagtcgg
     1561 ctaaatgaag tttactgtaa aaaaacgata ctttttcggg tggcgcttcc aatcagtaga
     1621 atatgtgttt ttcaatgaag gagccgatga cttttcgtgg atttccacgg aacgcgagaa
     1681 gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg agggtcaggt tgttacgttc
     1741 aagatatagc atgtgctgga tgaagatccc tttt
//
LOCUS       DADLSE010000313         1749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLSE010000313 DADLSE010000000
VERSION     DADLSE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.317"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..464)
                     /locus_tag="JN692_004299"
                     /pseudo
     CDS             complement(1..464)
                     /locus_tag="JN692_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154977.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            637..1419
                     /locus_tag="JN692_004300"
                     /pseudo
     CDS             637..1419
                     /locus_tag="JN692_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            1590..1703
                     /locus_tag="JN692_004301"
                     /pseudo
     CDS             1590..1703
                     /locus_tag="JN692_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052648.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
BASE COUNT          399 a          474 c          470 g          406 t
ORIGIN      
        1 ggtgcgcgcc ttcgtggcac actatcatcg gcgtaccttt cgaagggaac gtcgggacac
       61 tgacgttgca tccacatatc aaaggcatag tgcaggaaca gatttgagag caggggcgag
      121 atcacgccgc cctgcggcgt tccttctgtc accggcctta ccagacctgc gtccatcacc
      181 cctgcattca ggaacctgcg gataagtgac aacacccgtt tatccgatac tcgtttctct
      241 atccggctca tcagcacgtc gtgatttatc cggtcgaaga acttctccag atcgaggtcg
      301 accacccagt gatacccggc cccgatatat tcctgggcct gtatcactgc ctggtgggcc
      361 gaacgaccgg gacggaaccc gtagctgttg tcgctgaacg agctatctca cagtgcctgc
      421 aatacctgca tcatagcctg ctggatgaag cgatccacca ccatctggat gcccagcaga
      481 cgttcgccgc cgccgggctt cagtatggat actcttctca caggggatgg gcggtatctg
      541 ccggacagca gttgcgcttt cagctctggc cagtggtgcc tcaggtagtc cggcagttca
      601 cttacgcgca tgccatcgac tcccgccgca ccttgattgc gtcttgtcga tggaacagca
      661 atcagcgcgc ccgggggcgg cagcgctgaa tggcgactac atatgggata tgatcctcat
      721 acctgtcagt tcactgattt tgagctaacc gacagcagag acgctgaacg gctggatcga
      781 tttgcgcaaa cggcagacga gatacgaatt gctgaccggg gattcggttc gcgtcccgaa
      841 tgtatccgct cacttgcttt tggagaagct gattatatcg tccgggttta ctggcgagga
      901 ttgcgctggt taactgcaga aggaatgcgc tttgacatga tggattttct gcgcgggctg
      961 gattgcggta agaacggtga aaccactgta atgataggca attcaggtaa taaaaaagcc
     1021 ggagctccct ttcctgcacg tctcattgcc gtatcacttc ctcccgaaaa agcattaatc
     1081 agtaaaaccc gactgctcag cgagaatcgt cgaaaaggac gagtagttca ggcggaaacg
     1141 ctggaagcag cgggccatgt gctattgcta acatcattat cggaagatga atattcagca
     1201 gagcaagtgg ctgattgtta ccgtctgcga tggcaaattg aactggcttt taagcggctc
     1261 aaaagtttgc tgcacttgga tgctttgcgt gcaaaggaac ctgaactcgc gaaagcgtgg
     1321 atatttgcta atctactcgc cgcattttta attgacgaca taatccagcc atcgctggat
     1381 tttcccccca gaagtgccgg atccgaaaag aagaactaac tcgttgtgga gaataacaaa
     1441 aatggtcatc tggagcttac aggtggccat tcgtgggaca gtatccctga cagcctacaa
     1501 aacgcaattg aagaacgcga ggcatcgtct taacgaggca ccgaggcgtc gcgttcttca
     1561 gatggttcaa cccttaagtt agcgcttatg gggtaaatcc ccgccttttt cctgccggtc
     1621 cccggacaga gcacaaagga agtcgccgcc ggctccgctt tacccggcca tgcggggcat
     1681 ggccttgtgg gttttcagct ctgtagcctc agcgtcgtgt gcgggctgtg ccgtgcctcc
     1741 atcttagcc
//
LOCUS       DADLSE010000314         1696 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLSE010000314 DADLSE010000000
VERSION     DADLSE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.318"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..121)
                     /locus_tag="JN692_004302"
     CDS             complement(<1..121)
                     /locus_tag="JN692_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7266386.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILE"
     gene            449..524
                     /locus_tag="JN692_004303"
     tRNA            449..524
                     /locus_tag="JN692_004303"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:482..484,aa:Asn,seq:gtt)
     gene            complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN692_004304"
     CDS             complement(580..1515)
                     /gene="ldtA"
                     /locus_tag="JN692_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7266387.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN692_004305"
     CDS             complement(1577..>1696)
                     /gene="cobT"
                     /locus_tag="JN692_004305"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="catalyzes the synthesis of
                     alpha-ribazole-5'-phosphate from nicotinate mononucleotide
                     and 5,6-dimethylbenzimidazole; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7266388.1"
                     /translation="GAALAMSIIEAACAIYNNMGELAASNIVLPGNTTSDLNS"
BASE COUNT          449 a          428 c          372 g          447 t
ORIGIN      
        1 cttccagtat ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
       61 cctgggtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
      121 tgttgcctcc ggtttttaag aatcggccca agtgccgcca ttacttacaa ccagtatgca
      181 agatgcctgc cagttctatt ttggcgttga tgcgcaagct aaccaactgt caaataagag
      241 attatgatag attcgtcatt ggcccttttt atcagcagtc gcattcacct gccctataaa
      301 aggaaggtat gcaccacgat ggttcattac ccaataagat tgaaagctca ccactttgtt
      361 gaaattggca gcaaacaaac aaaaaaaatg catttcaccc tttgacatca ccatgcactg
      421 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
      481 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
      541 ctttcgagca ggctttttac tttctaatta ccaacgctct taaaacatct gtcttgaacc
      601 agaactaatt tgcacaggca ttcccgatcg atgttgcaac gcagcatttg cgcgatttac
      661 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
      721 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccactt
      781 tgaaccatcc ggttccgttg tgtatttcac tggctgatca ataatttgta cacgagtgcc
      841 tacaggaaca ttatcaaaca gatatttgat atcgtcatta cgcagacgaa tacagccctg
      901 acttacacgg agcccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
      961 aatataaatc gcgtacagcc ccatggggtt atcgggcccc gcaggaacaa atgcgggcaa
     1021 actctcccct cgtttcgcat attcgcgccg ggtgttcggc gttggcgtcc aggttggtgc
     1081 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     1141 gataccaata ggaaagactt ccactgtatt actgtctggc ggatagtaat aaagacgcat
     1201 ttcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     1261 aatggtgagt tgtgagccag gcttcggcaa aaaaacatca gcgcccggat tcgcttccag
     1321 catattactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gagtattgtg
     1381 atcaggaaca gttacagtaa gcgactgccc cactaaacgg ctaccctctg gaggtaatgg
     1441 ataagttacc gccaggctat tttggctggc aaaaagcaga gcaaatgagc aaagaatatt
     1501 tacacgacgc atcatgtccc tttcctaagt cgcgaatact atccgataag tatagctttt
     1561 atcagacttt tcgtttttag ctgttcaaat cagaagtcgt attccccggt agaacaatat
     1621 tactggcagc aagttcgccc atgttgttgt atatcgcaca agcagcttcg atgatggaca
     1681 tcgccagagc tgcacc
//
LOCUS       DADLSE010000315         1679 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLSE010000315 DADLSE010000000
VERSION     DADLSE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.319"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            236..505
                     /locus_tag="JN692_004306"
                     /pseudo
     CDS             236..505
                     /locus_tag="JN692_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            502..614
                     /locus_tag="JN692_004307"
                     /pseudo
     CDS             502..614
                     /locus_tag="JN692_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            757..1347
                     /gene="ospG"
                     /locus_tag="JN692_004308"
     CDS             757..1347
                     /gene="ospG"
                     /locus_tag="JN692_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7266389.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          495 a          351 c          391 g          442 t
ORIGIN      
        1 aaacggctca caactgcacc attcataatc acatcgcata aaactgtcag cgccagtgat
       61 aggacaggtg aaacctgtca atacgtaccg gcttacgccg ggcttcggcg gttttactcc
      121 tctgccatat gcaacaacgg agtgccgcct tacatgccgc tggcgcggca tatcctggtg
      181 acgatatctg aacagttata tactgtgtat atacgtggta atgacgaggt ggcaaatggc
      241 acaggttaat atgagtgtaa gaatcgacgc agaactgaag gatgctttta tggccgctgc
      301 aaaaagcatg gaccgtaatg gctctcagtt aatccgggat tttatgcgcc agaccgttga
      361 acggcagcac aattcttggt tccgtgacca ggttgcggca ggccgtcagt aactcgaatg
      421 cggtgatgtg cttccccatg acatggtcga atcctctgct gctgcatggc gggatgaaat
      481 gagcaggaag attgctgata aatgatggag atgttctgga ccatgctggc catccaggac
      541 aggaagcgca ttcgtagtac attgcggagc agaacctgat ggccgccatt gaaccggacg
      601 aacggattgg ttattcagcc agcagcctgg cagggcagcc gtataaaggc cgcaacggtc
      661 gtgtggaagg aacatctgga ccacacaagg tagcgtgtaa cgttattttg tgtgaaaatc
      721 tcttgtgatt tttaaatatc gtaacaaagg tatgacatga aaataacatc taccattatt
      781 caaacacctt ttccatttga gaataataat tctcatgctg gcatagtaac ggagcccatt
      841 ctcggtaagt taataggtca ggggtcgaca gcagaaatct ttgaagatgt gaatgattca
      901 tctgctttgt ataaaaagta tgatcttatt ggcaaccagt acaatgagat tctggaaatg
      961 gcttggcaag aatctgagct ttttaatgct ttttatggcg atgaagcatc cgttgttata
     1021 cagtatggcg gagatgtgta cctccgaatg ctgcgcgtgc ctgggactcc ccttagtgac
     1081 attgatacag ctgatatccc tgataatata gagagccttt atctacagtt gatatgtaaa
     1141 ttgaatgagt tgagtataat ccattacgat cttaatacag gtaatatgct gtatgataaa
     1201 gaaagtgaaa gtttattccc aatagatttt cgcaatattt atgctgaata ttacgctgca
     1261 accaaaaaag ataaagagat tatcgaccga cgattacaaa tgcgtacaaa tgatttttat
     1321 tcgttattaa acaggaaata tttatagccg tatttgttga tgctataagc cagaggctct
     1381 tctatgagaa tatggggacc attccctcgc gatattaacg taatatctta attgtatgca
     1441 ccgccagaat gcgttcagtg cgatcacggc agcagacagg taaaaatagc aacaaaccac
     1501 ccgaaaaacc gccgcgatcg cgtatgataa attttaacca catgcatacc tgtgcagcaa
     1561 ggcgaatcac gcacggattt ccagccagag acacctgaaa cgtaaccggc tcatttaaac
     1621 cgtctggtct gttacctccg gttccacaaa aataatgtcc atcattttga agtggacac
//
LOCUS       DADLSE010000316         1636 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLSE010000316 DADLSE010000000
VERSION     DADLSE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.320"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..1005)
                     /locus_tag="JN692_004309"
                     /pseudo
     CDS             complement(1..1005)
                     /locus_tag="JN692_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481272.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(1340..1558)
                     /locus_tag="JN692_004310"
     CDS             complement(1340..1558)
                     /locus_tag="JN692_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266390.1"
                     /translation="MFKCNCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKV
                     DVLILIFGYINALFLGVFMAVMCIEYWR"
BASE COUNT          448 a          390 c          348 g          450 t
ORIGIN      
        1 gcactgcttt tgtactggaa agtgagattt ttagacagaa cgcgcttcgc ctgtaggctg
       61 aagatatacc ccagttcctc ttcagaatgg tgcacatcaa gatgagcatt atcagtagta
      121 cgaggcgacg tagcgaaccg gttgttatag gcttcaataa aggtcggcaa ccatgcattt
      181 gctgtttcaa tatcactgat attctgaagc cgcatttctt tgaccagcct gtcctgtagt
      241 gtctgattgg cgcgctctac ccgccctttt gcctgcgggc tgttggcatg gattggctcg
      301 atgcccagtg tctttatcgc acgagtgaac tgggtcagct caccttcccg ctctgggtta
      361 tttaccctga atatactgtg tctatcagag tagagagcga gcggtacgcc atgatcatta
      421 aggtaacccc ggagggtttc catgtaagcc cgggttgttt cagcaggcac aaaacgcaac
      481 gccatcaacg cactggtggc atcatcaatg aaaacgatca gtgtacatct ggggcctcga
      541 ttttcaaacc agtcatgagg tgagccatca atctggatca gttcaccgta agatggtcgt
      601 cgcatgcggc gttgatatat acgggcaatt ttacgacggc gttcacgcca taccccctct
      661 tctatcatcc actttctgag agtttcaacg gataaagata aaccgtgtat ctcgcgcaat
      721 ttttcgcacg caaacgtagg tccaaaatca gcgtaacggc ctttgaggag tgaaattact
      781 gttgctctga attcagtaga aaaggaatta ttaggacgct ttccacgtcg gtgggaaacc
      841 agaccagaag gcccttcatt tctataccgt tgcaccagac gtttaactag aaatgccaat
      901 gcgtgccgca gcttgttcct gagtaatatg tcgattaatt gactcctgaa taatctgaag
      961 tcggtggagt tccttatgac tcatcgtaac agtctctttg atcatgaaaa atcccccaga
     1021 gaattgtctg gggacatttt agaatggttc aaaggggaca ttacagcttg gtgttaacaa
     1081 tagcctgctt ctccttgcct ttcggcacgt aagaggctaa cctacatgtg tctggcatga
     1141 aattggcctc agattaatgt taagcgtctt gcaggacgcg taatgttaac tggggctttt
     1201 ctctacctgc cgttggtgtt cgtgcccgag gcagatagcc tcaagcaccc gcagtcattc
     1261 tacttaacta agatttcccc gcaaaccgtt tttgtccagc acagtaaata tccaactaaa
     1321 ccaatggagt tcgctgtatt taccgccagt attcaatgca catgaccgcc atgaacaccc
     1381 ctaaaaaaag ggcatttata tatccaaata ttaatatcaa aacatcaact ttttccatat
     1441 accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca acaaacaaaa
     1501 actgcaatgc gtttttcatt attcctccta caatcaatgt gcaattacat ttaaacacac
     1561 ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca tagcgttaca
     1621 aactctttag tctaaa
//
LOCUS       DADLSE010000317         1630 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLSE010000317 DADLSE010000000
VERSION     DADLSE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.321"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..1046)
                     /locus_tag="JN692_004311"
     CDS             complement(<1..1046)
                     /locus_tag="JN692_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7266391.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1538..>1630
                     /locus_tag="JN692_004312"
     CDS             1538..>1630
                     /locus_tag="JN692_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HAY7266392.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIV"
BASE COUNT          480 a          270 c          346 g          534 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaggac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atcttaatgt actgtttttt tctttaaaaa aacaatacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct ccccccccga
     1501 cttttgacgc agtgtcttgt taacaggggg atttaccatg cttattggct atgtacgcgt
     1561 atcaacaaat gaccagaata ccgatctaca acgtaatgcg tttatttgtg ccggaagtga
     1621 gttgattgtt
//
LOCUS       DADLSE010000318         1616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLSE010000318 DADLSE010000000
VERSION     DADLSE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.322"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..196
                     /locus_tag="JN692_004313"
     CDS             <1..196
                     /locus_tag="JN692_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7266393.1"
                     /translation="ALNQLLEARTQLANLQTYMDGKAGAENLINKLLQDPTLLKALAS
                     APKPVAQQESVLSGETGTAD"
     gene            221..>1616
                     /gene="tssC"
                     /locus_tag="JN692_004314"
     CDS             221..>1616
                     /gene="tssC"
                     /locus_tag="JN692_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HAY7266394.1"
                     /translation="MVNDTLLATGDLSAAQTTAYQDTEFDSLLDKAFRPKTPQAAKAV
                     EAAVQTLAQQALTNTVTVSDDAYKSIAAIIEQIDFKLTEQIKLILSHPEFQKLESSWR
                     GMEHLVYNTETDEKLKIRFMNLSKDELRKNMKRYKGIAWDQSPMFKKLYEAEYGQLGG
                     EPYGCIIADYYFDHTPPDVDLLGSIAKVAASAHAPFIAGASPSVLQMDSWQELANPRD
                     LIKIVTQNLEYAPWNSLRKSEDSRYIGLTMPRFLSRLPYGAKTNPVDEFDFEEDADGA
                     DHSKYVWSNAAYAMGVNINRSFKHYGWCTLIRGVESGGAVENLPCHTFPTDDGGVDMK
                     CPTEIAISDRREAELAKNGFIPLIHRKNSDYAAFIGAQSLQKPQEYYDPDATANANLS
                     ARLPYLFACSRFAHFLKCIVRDKIGSFKEREDMQRWLNEWIMNYVDADPVNSSQETKA
                     RRPLAAAEVVVEEVE"
BASE COUNT          436 a          389 c          395 g          396 t
ORIGIN      
        1 tgccctgaac cagctgctgg aagcgcgtac tcagcttgcc aacctacaaa cgtatatgga
       61 tggtaaagcg ggagcagaaa atcttatcaa caaactatta caagacccta cgttactgaa
      121 agctctggcc agtgcgccaa aacctgtcgc tcaacaggaa agtgtcttat caggtgaaac
      181 gggaacagca gactaactgc gattttatta aggaactttc atggtaaacg atactcttct
      241 ggcgactggc gatctctctg ctgcgcaaac cactgcctat caggacactg aatttgattc
      301 gctcctggat aaagccttcc gtcccaagac acctcaggca gcaaaggctg ttgaggcagc
      361 agttcagacg ctggcccaac aggctctgac aaataccgtc accgtcagtg acgacgctta
      421 taaaagtatt gctgcgatta ttgagcagat tgatttcaaa ttaactgagc agatcaaact
      481 catcctgagt catccggaat ttcagaaact ggagtcctcc tggcgcggga tggaacattt
      541 ggtttacaac actgaaaccg atgaaaaatt aaaaatccgc tttatgaatc tttcaaagga
      601 tgaattgcgg aaaaatatga agcgttacaa agggatcgcg tgggatcaaa gccctatgtt
      661 caaaaagctt tatgaagctg aatatggaca gttaggtgga gaaccttacg gttgtattat
      721 tgcggattat tattttgatc acacgccacc ggatgtggat ctgctcggtt ccattgccaa
      781 agtagcagcc tcagcccatg cgccgtttat tgccggggcc tctccgtcag tgttgcagat
      841 ggactcctgg caggaactgg ctaacccgcg tgatttgatc aaaatcgtga cccaaaacct
      901 cgaatacgca ccgtggaact cgctgcgaaa aagtgaggat tctcgctaca ttggcctgac
      961 gatgccacgt tttctctccc gtttgcctta tggtgcgaaa acaaaccctg tcgatgagtt
     1021 tgattttgaa gaagatgcgg atggcgcaga tcacagcaaa tatgtctgga gcaatgccgc
     1081 ctatgcgatg ggtgtaaata tcaaccgttc tttcaaacat tatggttggt gtacgttgat
     1141 ccgtggtgtg gaatctggtg gcgcggtgga aaatctgccc tgtcatacct tcccgaccga
     1201 tgacggcggc gtggatatga aatgcccgac agaaattgcc atctctgacc gtcgtgaagc
     1261 tgaactggcg aaaaatggtt ttatcccact tatccaccgc aaaaactctg attacgccgc
     1321 ctttattggt gcgcagtctc tacaaaaacc gcaggagtat tacgatccgg acgctacggc
     1381 caacgccaat ctttcagctc gtttgccata tcttttcgca tgttcacggt ttgcgcactt
     1441 cctcaaatgt atcgtgcgag acaagatcgg ctcgttcaaa gagcgtgaag atatgcagcg
     1501 ctggctcaat gagtggatca tgaactacgt cgatgccgat ccggtgaact cctcacagga
     1561 aaccaaagct cgtcgcccac tggcagctgc tgaagtagtg gtggaagagg tggaag
//
LOCUS       DADLSE010000319         1615 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLSE010000319 DADLSE010000000
VERSION     DADLSE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.323"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            66..458
                     /gene="ydeI"
                     /locus_tag="JN692_004315"
     CDS             66..458
                     /gene="ydeI"
                     /locus_tag="JN692_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HAY7266395.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            713..1603
                     /gene="dgcZ"
                     /locus_tag="JN692_004316"
     CDS             713..1603
                     /gene="dgcZ"
                     /locus_tag="JN692_004316"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HAY7266396.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSYMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT          454 a          312 c          387 g          462 t
ORIGIN      
        1 tttggcaatt ataaaaatag caaccagact taataagtca aaccaaacgc aattaaggag
       61 ttagtatgaa atttcagtcc attgtattag caagttttct tgtcatgcct tatgcgttgg
      121 cagatgacca gggcggttta aaacaggatg cagcgccacc gccgcctcat gcaatagaag
      181 atggctatcg cggaaccgat gatgcaaaaa aaatgaccgt tgatttcgca aaaaacatgc
      241 acgatggcgc ttcggtttca ctacgcggta atttgatttc tcacaaagga gaggaccgtt
      301 acgtttttcg cgataagagc ggtgaaatta atgtcgttat tcctgcggcc gtctttgatg
      361 gacgagaagt tcagccggac cagatgatca acattagcgg cagtctggat aagaaaagcg
      421 cgccggccgt cgttcgggtc actcatttgc agaaataaaa ataaattagc ctgatggcct
      481 gaactactgg gccattaggg tattgggtta tgctatttgt catcctttta tttacgataa
      541 tgcagaggtg atttacagaa ttaaacactt ttttatccgc tgattttgtt ttaaatcaca
      601 atgttatctc agagatgact cataacttcg gatttctatg aaaatattga cgtaattttg
      661 cttttctctc gttagaatag cgcgcacaag gaactgtgaa aaaggagtgg caatgatcaa
      721 gaagacaacg gaaattgatg ccatcttgtt aaatctcaat aaggctatcg atgcccacta
      781 ccagtggctg gtgagtatgt ttcacagcgt ggtcgcgaga gatgccagta agccagaaat
      841 aacggataac cattcttatg gactgtgcca gtttggtcgg tggattgatc atctggggcc
      901 actcgataac gatgaattac cttacgttcg gctaatggat tctgcccatc aacatatgca
      961 taactgtggt cgggaattaa tgctggctat tgttgaaaat cactggcagg acgcgcattt
     1021 cgacgccttt caggaggggt tgctttcttt tactgcggca ttaaccgatt acaaaattta
     1081 tttgctgacg atccgtagct atatggatgt tttgacggga ttgccggggc gtcgggttct
     1141 tgatgaatcc tttgatcatc agttacgcaa cactgatcct ctgaatcttt atttaatgtt
     1201 gttggatatt gaccgattta aattggttaa tgatacctac gggcatttaa tcggcgatgt
     1261 agtattacgc accctggcaa cttacttagc cagttggacg cgtgattacg aaacggttta
     1321 tcgctacggg ggcgaagaat ttatcattat tgttaaagcg gctaatgatg aagaagcatg
     1381 tcgtgcaggt gtcagaattt gccagttagt cgataaccat gccatcacac attctgaagg
     1441 gcatatcaac attaccgtga cagcaggtgt gagtcgcgca tttcctgaag agcctctgga
     1501 tgtggtcatt ggaagagcag accgggcaat gtatgagggt aagcaaaccg gaagaaatcg
     1561 ctgcatgttt attgacgaac aaaatgtgat taaccgagtt taagctccgt ttaac
//
LOCUS       DADLSE010000320         1610 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLSE010000320 DADLSE010000000
VERSION     DADLSE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.324"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(251..1610)
                     /locus_tag="JN692_004317"
                     /pseudo
     CDS             complement(251..1610)
                     /locus_tag="JN692_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
BASE COUNT          572 a          229 c          282 g          527 t
ORIGIN      
        1 ttatgtaatt aggcggttaa actttaatat tcgcgttaag tttgggtaaa cgcttgtaaa
       61 tggtatcgct aattctttaa ttgactaata tttgaaacgg ttagtgtgat tgtatataaa
      121 tatttgagtg taaggtattg aaaggggcat tcagcccctc cgtctatttg tgattgcatc
      181 cattgatgcg cgtattattt tgagcttgaa aaagggaggc gctctttaat aataaataaa
      241 tctatcaaag ctaaatatta aatggaagcc accctttttt ctctgatagt tttattatca
      301 ccaaaccatt tatttcagtt atcgaatctg gattctgcca ggaaggtaaa ctcttcacaa
      361 ttggataaat cttctctatt tgttctttat ttgctggttt gcaattacag attccatagt
      421 aattcataaa tgcaaccatc ctaaaatagt tgccaccatc ccaccaaaaa aagattttcc
      481 aaaagtgtct gcgctcatgg agagcatatt tcctgagtcg tttgaaccat gtatatatat
      541 gtattttccg ttaagggaaa tgccttttgc ttgagatata tatgatattt ctttagctaa
      601 agagatatct ttctgtcttg caagagtatc attcaaaaat agatttttgg atattgaaac
      661 gctattaaat ataataaata aaaaaacatg tagtttaggt gttttatcga gtgaaaattg
      721 cttaagcaag aaaacaaaat tactgctact attggcatta aaacaaacag gcgaggtagc
      781 gcccctgagc caacaacaac gataaatata accggaagta tataaataaa aatgattgat
      841 gaaatcaaat aaattgattt tgtttttaat ttatatatta gggtgaatat cagaactaat
      901 aaaggaatca gtaaccactt gtataagttt aaaccagtag gaggattgtt atagaagtta
      961 tatatattat ctatggctga tttaacaagc catttaatat cgcttgcatt ttgtatgtaa
     1021 tttgagatga actcgttcga ttcaagcgaa aaataatgct tgatagcttt ggttaatagc
     1081 aggtaaatta aggtggataa agctatgagg gatagagata aacatgaaat ataaaaagac
     1141 tcacgaatat ttttctcttt tcgtattatc gaatttacct gcaacccaat gacgaatgca
     1201 ataatatatg ttacgaatgt ttggtagatt accattgaaa gaatggacag tactatacca
     1261 gaaaatatca caatcctatt tttttgcgag tgaaaaataa ttgctgatat cgctgccagt
     1321 agaaaagcaa ttcccaccgt atcagcttgg ttaagaaact ctaattgata ggatatctga
     1381 ggaaaggtaa taaatactaa catacctatc aataattcat aagattctag ctttagcgat
     1441 ctgcagatta taaatgctga agtgatgata aaaaataaag ctactaatgg tgttatataa
     1501 agtgaaaaag gctcagggag aaaataatgt cgcaaaaaag tgtgaaacca ccgtccaagc
     1561 gtgattgttt gataaaaatt atttgtaaac tcgccatcaa tattaagtgt
//
LOCUS       DADLSE010000321         1599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLSE010000321 DADLSE010000000
VERSION     DADLSE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.325"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..399)
                     /locus_tag="JN692_004318"
     CDS             complement(<1..399)
                     /locus_tag="JN692_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HAY7266397.1"
                     /translation="MSRLVVVSNRIAPPDEHAGSAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYH"
     gene            complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN692_004319"
     CDS             complement(374..1174)
                     /gene="otsB"
                     /locus_tag="JN692_004319"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HAY7266398.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN692_004320"
     CDS             complement(1341..>1599)
                     /gene="araH"
                     /locus_tag="JN692_004320"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HAY7266399.1"
                     /translation="FSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILG
                     TVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT          389 a          430 c          378 g          402 t
ORIGIN      
        1 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
       61 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcaccagatc
      121 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      181 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tcaccttttt
      241 tagcggctga tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgcggc
      301 tttcagtgcc cccagtatgc caacggcaag gccaccggca ctgccggcgt gctcgtctgg
      361 tggtgcaatc cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt
      421 ctcttttttg ttgtaatgcg gtggttatca tttcaagcca gctccagaca tccggcacac
      481 ccgccagtcg ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca
      541 gtcggttaac gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg
      601 ttcgcccgat aaaaggagct tcctgcataa aggctgcaat cgcttcacct ttactggtac
      661 ctctcggttt gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct
      721 gagtaatacg ttgcgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat
      781 gcagcgcaaa agccatccct ttcgcctcca gctccgcgcc gggatactga gcgatgaccg
      841 tatgcagttg cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgtgttt
      901 taccattgat gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt
      961 ttgccagtgc gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac
     1021 ttgcggttgc cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg
     1081 gatgcggttt gatttccgcc agcgttccat caagatcaaa aaaccaggca tatttcgcgg
     1141 atagttcagg ggtttcggtt aacggttctg tcacccggct ctcctccttc tcttttctta
     1201 ttgtgttgcg cagaccgcgc tttacagaca tgtaagtata gacagtgtga cggaggtcgc
     1261 catttgcaac gaagaacagc caaccgttgt ggcggctggc tgtggtggga aaaaacgtta
     1321 aattgttgtg gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag
     1381 atcaccgctg ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta
     1441 agcaggttca tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag
     1501 atttttccga tgccaccttt cagagaaacg ccacctaaaa cgcaggcgga gataacaata
     1561 agctcataac caatcgacgt cattggctgc ccactaaat
//
LOCUS       DADLSE010000322         1596 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLSE010000322 DADLSE010000000
VERSION     DADLSE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.326"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            3..386
                     /locus_tag="JN692_004321"
     CDS             3..386
                     /locus_tag="JN692_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7266400.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(387..1295)
                     /locus_tag="JN692_004322"
     CDS             complement(387..1295)
                     /locus_tag="JN692_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7266401.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERPGFQVYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            1395..>1596
                     /locus_tag="JN692_004323"
     CDS             1395..>1596
                     /locus_tag="JN692_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HAY7266402.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYS"
BASE COUNT          423 a          354 c          381 g          438 t
ORIGIN      
        1 tggtgctggt ggcaattctg attatgctgc cgactctcgg catcggtgat atgaacaatc
       61 tctataaatg gctgttgaat cttaactcgg tagtgatgcc gctgcgttat ttatgggtat
      121 ttgttgcatt tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta
      181 ttcgtaataa gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctgtg
      241 cctgtttgac ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt
      301 tggcgctgaa tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc
      361 tggcgcgtaa agcgaatagc aaataattat tgtggtcggt gacgctgact ctggcagatc
      421 tgacccgttt ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt
      481 gggtaataga cttggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa
      541 cgaccagtat caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc
      601 atcaaaacgg cctccagttc agcatcaaca tcatccagaa tgatatttcc tgcgacagca
      661 atttccagct cctttgcaaa ttgccagtgg aaaggtttgc cgctgggata acgaaaaaca
      721 acacattgat gattaagcag atcatgagga tggcggggtt tgccatatct tgcaaagtac
      781 tctggcgttg ctgctacaca taatttcact ggtgggccga tcgccacgct gatcatatct
      841 ttttcgacga tgccactaag tcgaacgcca gcgtcaaagc cctgttggac gatatcgata
      901 agagaatcgt cagttgtgag ctctacttta atatcaggat attcacgtgt gaaaccaacc
      961 agtaaaggca ttaaaaatat acgtgcagct acccgggcgg cattgatttt gagcgttcct
     1021 gtcggtgtca gacgaaaatc attcatctca tctaacatga tctgaatttc gtcgaaagct
     1081 gggcgcaatc gttcatagag attagaaccc gcttccgtta acgatacgct tcgggttgtc
     1141 cgattgaata agcgaatttt aagacgttgt tccagtgttt taatactatg gctaatggcg
     1201 gacgaggata agcctaactc atcgcctgct gcacgaaagc tttgattacg ggccacggca
     1261 aagaaagtgg caaagtcaga catattcatc cgcattagtg aaaatccttc attaactcat
     1321 cctgaatcat cacgtttaca ggattatgcc gcctgcgtaa agtgcgctcc agaacttaac
     1381 gtggaggtaa aattatgcag tctgaacgta tttatttggt atgggcccat cctcgtcatg
     1441 attcattgac cgcaaatatt gctgatgcga tccatcagcg ggcaatggag cggaaaatac
     1501 aggtgacgga actcgattta tatcggcgta atttcaaccc ggtgatgacg tcggaagatg
     1561 aaccagactg gaagaatatg gataaacgtt attctc
//
LOCUS       DADLSE010000323         1588 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLSE010000323 DADLSE010000000
VERSION     DADLSE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.327"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..206)
                     /locus_tag="JN692_004324"
     CDS             complement(<1..206)
                     /locus_tag="JN692_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAY7266403.1"
                     /translation="MDDIVNSVPSWTFTAIIAVCVLFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIP"
     gene            complement(233..862)
                     /locus_tag="JN692_004325"
     CDS             complement(233..862)
                     /locus_tag="JN692_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HAY7266404.1"
                     /translation="MNLFADHNTPYLFAISFVLLIGLLEIFALICSHMLSGTLDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLQEPEEGKIFTKGDKVLIICRLSATRYLVENNPWPQIL"
     gene            1131..1331
                     /gene="glgS"
                     /locus_tag="JN692_004326"
     CDS             1131..1331
                     /gene="glgS"
                     /locus_tag="JN692_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7266405.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(1374..>1588)
                     /locus_tag="JN692_004327"
     CDS             complement(1374..>1588)
                     /locus_tag="JN692_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7266406.1"
                     /translation="LDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRA
                     ELSANPAETVVEGPFSKDVILKITYN"
BASE COUNT          488 a          303 c          355 g          442 t
ORIGIN      
        1 gggattattt catgaaagat cggcatcacg attgcgccac cgctcatcac cactttttgc
       61 ccacctaaac cagtacgaac aaatgcttgc tctgccgaag cgcgacgata gagcctggcg
      121 aaaataattc caataataaa cagaacgcat acggcaataa ttgcggtaaa cgtccaggaa
      181 ggcacagaat taacgatatc atccattatt aaatttcctt ttgattaata aattacaata
      241 tttggggcca gggattgttt tccaccaaat atcgagttgc agaaagtcga caaataatta
      301 acactttgtc tcctttagtg aaaatttttc cttcttcagg ctcctgtagt aaataatgta
      361 tttggccaaa ttgatcggtg agttttcctt cgcagggatt acctgaagtg gcttgatgac
      421 cggtaattaa cgccatgcta ccaatatatt cttcttctgt aatagctgaa ctatggtcgc
      481 gtggaatcca gggcgcaact attttaccgg tataatgcac cgcaataatc gtaaacagta
      541 aactgacagg aaccacgaac aggttcgaca gtggtgattg ccagaccatg atgcaggcat
      601 gttgcaacag gatgccaata agaccaaaaa aaccagcgag taagcagaga acgacgagag
      661 ctggcaatct tccgatattg agataatgaa gtgcctggct aatatgacct gtggttatgg
      721 aatcataatg gtcaagatgt gcatcaagag taccggagag catgtggcta cagataagcg
      781 caaaaatttc taaaagaccg atcaacagta caaaggaaat agcgaaaaga taaggggtgt
      841 tatggtcggc gaataaattc atatgtcact ccaatgtcca tttagtgacg caatatttac
      901 cttggaatag tttccctgct tacatttgtc tttgttaata ataacacatg ttagagaagt
      961 tctggaagag aaagtatttt aatatggttt tttatttttc actaaaaagt gtgatcgggg
     1021 acaatatatt tacgcacgtt atgtttaaag gcactacact gattggggaa tactgaaatc
     1081 agaaaaagca tatggcctta aaaggtatgc gataaccgga ggatgtgctt atggatcata
     1141 gtcttaattc tttgaataat ttcgatttcc tggcgcgtag ttttgccaga atgcacgcag
     1201 aaggtcgccc ggtcgatatt ctggccgtta ctggtaacat ggatgaagaa catagaacct
     1261 ggttttgcgc acgttatgcc tggtattgcc aacagatgat gcaggcaaga gagctggagt
     1321 tagagcactg atataacggg cctgatggcc cgttttagtg tttgtattag atgctagtta
     1381 taggtgattt tgaggattac gtctttacta aacggtccct caacaacagt ctctgccggg
     1441 ttcgcactaa gttctgccct gaatggtgcg gtgaccgaag gtgcactaga cgatgaaaaa
     1501 gaagcaaatt gtgagaagcg gttaaattcc gttgctgttc catctttatg aaatatcttc
     1561 aatagcagtc cattacccat atccagat
//
LOCUS       DADLSE010000324         1580 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLSE010000324 DADLSE010000000
VERSION     DADLSE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.328"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..780)
                     /locus_tag="JN692_004328"
     CDS             complement(<1..780)
                     /locus_tag="JN692_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7266407.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
     gene            complement(1381..>1580)
                     /locus_tag="JN692_004329"
     CDS             complement(1381..>1580)
                     /locus_tag="JN692_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7266408.1"
                     /translation="GAVDALKEELTRRISKRYDDVEVIVKATSNDGLSVTRTADKDSA
                     KTFVQETLKDTWESADEWFVH"
BASE COUNT          416 a          269 c          378 g          517 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct attgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta atgttgtcag gcctaactca gacaagtcca ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt tattcttctg
     1141 gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt gttttgctga
     1201 gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca tgattggcgg
     1261 gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc ggctggctgg
     1321 tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt tacgtgctaa
     1381 ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt cctgaacaaa
     1441 agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt tgctggtggc
     1501 ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta attcttcctt
     1561 taacgcatcc acagcaccgg
//
LOCUS       DADLSE010000325         1577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLSE010000325 DADLSE010000000
VERSION     DADLSE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.329"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN692_004330"
     CDS             complement(<1..174)
                     /locus_tag="JN692_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7266409.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            173..1312
                     /gene="queG"
                     /locus_tag="JN692_004331"
     CDS             173..1312
                     /gene="queG"
                     /locus_tag="JN692_004331"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7266410.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1295..1348)
                     /locus_tag="JN692_004332"
     CDS             complement(1295..1348)
                     /locus_tag="JN692_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266411.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          407 a          388 c          406 g          376 t
ORIGIN      
        1 aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca tcagctcata
       61 gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
      121 ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
      181 gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt
      241 tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg
      301 gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc
      361 tcgcccccat gagttattac ccggtacgct gcgtgtaatc agcgtgcgga tgaattacct
      421 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
      481 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
      541 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
      601 tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
      661 cctcaatcgc gaggccggtt cgttcttctt tttaggtgaa ttgctggtcg atattccgct
      721 gcccgtggat caaccagtcg aggaaggatg cggcaaatgc gtggcctgta tgacgatttg
      781 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
      841 caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
      901 ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac
      961 agaagacgat ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttatttgc
     1021 ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc gtataggtca
     1081 cctgcgctgg ctgcgtaata tcgccgtggc attaggcaat gccccttggg atgaaacgat
     1141 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc acatagcgtg
     1201 ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc aattgccgaa
     1261 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
     1321 cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga
     1381 tcgactcgac aaaacggaat cttgattttt aacttattta tcaataggtt aaaaaatacc
     1441 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
     1501 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat
     1561 attttattga gagaaga
//
LOCUS       DADLSE010000326         1547 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLSE010000326 DADLSE010000000
VERSION     DADLSE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.330"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..1547
                     /gene="yjcS"
                     /locus_tag="JN692_004333"
                     /pseudo
     CDS             1..1547
                     /gene="yjcS"
                     /locus_tag="JN692_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
BASE COUNT          394 a          423 c          408 g          322 t
ORIGIN      
        1 tgcgcgatgc taatggcaaa ccatattatc gcggggaaga ttataaattt gatatcaatg
       61 cgcccgcgcc cgaaaccgtt aaccctagcc tgtggcgtca gtcgcagtta aacggtattt
      121 ccggcctgtt taaagtcacc gacagaatgt actaggttcg cggtcaggat atctccaaca
      181 tcacctttat cgaaggtgat accgggatta ttgtcatcga cccgctggtg acgccaccga
      241 gcgcaaaagc cgcccttgac ctttacttcc aaaatcgccc gcaaaaacag attgttgcgg
      301 ttatttatac ccacagccat accgaccact atggcggcgt gaaaggcatt atctctgaag
      361 ccgatgttaa atcaggcaaa gttcaggtga ttgccccagc aggctttatg gacgaagcca
      421 tcagcgaaaa cgtgctggcg gggaacatca tgagccgccg tgcgctctac tcttacggtc
      481 tgttactgcc gcacaacgcg caaggcaacg tgggtaatgg ccttggcgtg acgctggcaa
      541 cgggcgaccc gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga
      601 ttatcgacgg cctggagttt gacttcctga tgaccccagg tagcgaagca ccagccgaaa
      661 tgcacttcta tattccggcc ctgaaagcgc tgtgtaccgc cgagaacgcc acgcataccc
      721 tgcacaactt ctacactctg cgcggcgcga aaacccgcga caccagcaag tggaccgagt
      781 atctgaacga aacgctggat atgtggggta acgacgcgga agtcctgttt atgccgcaca
      841 cctggccggt ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacta
      901 tcaagtacat tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa
      961 tcggcgacat gattaaactg ccgcctgcac ttgccaataa ctgggccagc cgtggctatt
     1021 acggttctgt cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg
     1081 gtaacccggc taacctgcat ccgtatggtc aggtggagat gggtaaacgt tacgtgcagg
     1141 tgctgggcgg ttccgcacgc gttattaacc tggcgcaaga agcgaacaag caaggtgatt
     1201 accgctggtc ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg
     1261 cgaagaatct gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat
     1321 ggcgcggttt ctacctgacc ggcgcgaaag aactgcgcga aggggtgcat aagttcagcc
     1381 acggcaccac cggttccccg gacaccattc gcgggatgtc ggtcgaaatg ctgttcgact
     1441 ttatggccgt tcgcctcgat agcgcgaaag ctgcgggtaa aaatatcagc ctgaacttca
     1501 atatgagcaa cggcgataac ctcaacctga cgctgaacga tagcgtg
//
LOCUS       DADLSE010000327         1509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLSE010000327 DADLSE010000000
VERSION     DADLSE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.331"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1008
                     /locus_tag="JN692_004334"
     CDS             <1..1008
                     /locus_tag="JN692_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266412.1"
                     /translation="VGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAAR
                     KVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQ
                     SVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEM
                     IKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETL
                     EGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSL
                     TPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMG
                     V"
BASE COUNT          461 a          283 c          342 g          423 t
ORIGIN      
        1 gtaggtccag atatacttgc attgcccgtc aacgtatatc atgcggattt taaaattaaa
       61 atcaataatg cagtggagaa ggcttcatca attgtgttat gtgctgtgca aaaaaatctc
      121 catgcagcaa ggaaggttct tagcgggata ataggaaatg tgccaaaaaa taataaacca
      181 aaaacaatta tttttgattt gcgttttctt agtgcaatta agcagaaagt ttttttgggc
      241 agtgaaaaac cgttgtttgt aaagcatacc gcaatgatga gggaggcatg taaggaatta
      301 ccgcagtcag tggaatatgt tcctttagcc tgtgaagcgc atagccatcg cagcgtcgcc
      361 cttgccaaaa ccgtggctgc gcccatcatg gcgacaggtg tgggcttggc attaatgtat
      421 gcaacatcat caggaccgta ttatgcgcaa agtctttctg gttcgccgga tatcgagatg
      481 ataaaaaatc gcatggctga attgtttaac cagtcggata tttctgtgcg caacaaatat
      541 agcccggctt ttgataaatg gaatgaactg gtagataaat taaatcacga aagaaataca
      601 gttccgtttg cgtgtttgac caccatttta gcgacggcga taaatgaaac tcttgagggg
      661 gatacgaatg ttgccgttgt gatggggtgt aaatctgcga aagatcgcac gatatccatt
      721 gtccttggca actcgatgtt gcaaacactc cttgaaaaac gcctcgccga tagtggagaa
      781 atagaaaatc tttttgatca acagggatat tttagttgcg attcattaac acccgaagaa
      841 ttaatgatgt taaaagacct ttttgatatt cgagttcttc atgtctctaa caaattcaat
      901 gttggattgc aaggtaatat caacactgat gttttacaag attcgttttt taaaaatgtc
      961 gatttcattc atgaatataa ctcgtatacc gtagggatgg gagtctgacg agtaaaactc
     1021 attaggatta tcagaattcg gacaatgcat tgcaaaaatg acatacaaag agagatgtgt
     1081 gcaggtactt agccgcgagg ggcatggcga agccatacag aaagtaccag tattcagaac
     1141 tccagtgaaa gcggcatagt ggccgcctgc tcaggagttt tgctatgacc cgtaatcgtg
     1201 ctgaacgtcg ccaccctatg atgcgattaa aaaaactcat cgtcgcgctg atgattgacg
     1261 tcaatgctat cggatgatgc cgggggtgtt ccggaaggaa ttccttgttt cccgaactgt
     1321 taccaacaaa attataacga tgagcaagta aagaccggtt ggtggcagta ctgaccggtc
     1381 tgtgatgaaa gattaatttc accttaaatc ctggtgctgt taggatgctg acgtttttcg
     1441 gctacaccat tcagattatg caaataaata tccctgaata taatatattc atagcaccca
     1501 taactcatc
//
LOCUS       DADLSE010000328         1505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLSE010000328 DADLSE010000000
VERSION     DADLSE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.332"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(877..1353)
                     /locus_tag="JN692_004335"
     CDS             complement(877..1353)
                     /locus_tag="JN692_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7266413.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          353 a          398 c          376 g          378 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcagcgtaa aagatctatt ctaggcaagg gtgttgcgtt
       61 gcacgcaaca ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccatccgtt
      181 caccgttaaa acgcctcaga aatcgggaca ggatgtgtaa tcgtataacc gcgcatgcac
      241 gccgatacga ttaccacttg gtcagggctt caccccgaca ccccgcatca gtgcgtaagt
      301 gctcaatgtg gcttccggtt ctcttccgct tcctcgtcga ttcgctcgct acgctcggtc
      361 atcgacttgc ggcgagcggg atcacctctc gcatgttgtc ggtgatcccg gtgttgttct
      421 ttcggggatg agtcgccaga tagcccctca aacgcgctca aaagctctca gacgcacgaa
      481 acgggtttta gatgcccgcc agggcatgga aggcgcgaaa aacgcttaca gaggctctca
      541 ggaggtcagg cgcgccgata cccctaacca ggggctttca ggtcgccagg aaccgcgcca
      601 gccccctgca aagcccattt gcacggaaca ggcagccatg cggttggtag gtggtcgtag
      661 accgacggtg ttgcagcgat gggggtctgg ggttctcccc agacggtttc cggtagcgct
      721 caaggcgctg ccggaaaccg tggcggcgcg gttttttctg gagaacgggg agcaaaaaca
      781 cgcaaaggat tttgaggcgg caaaagagca atgcaggata gacaatcatg gtcagaacgt
      841 taacacgtag cccctgattg tccgcctgcc gctagctcaa tttgaaaaca tggccatgat
      901 tcggcgctga atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ctgcatggtt
      961 tttcgtagtg ttgtatccct tccagacttc atgtggatcg agggcatacg cacaaacctt
     1021 accagcaacg gggattctaa ctaatgcatt ttttgattct aagtaattga gccaccgaat
     1081 aattgtgctt ctgctaactc caagtttttc agctaggaat tgctgatccg caacgacagc
     1141 gccacacgta ggatcgatat gctccgctaa aaatgagtac agcttcagtg ctgatatgcc
     1201 ttggctatcc ttcagcagct ctcgaacgcg ttcccaacct tttggggata cctgtgtaaa
     1261 ccttgaattt ttctttgctt cttcgagatc ctcggtgttt ttctttgcct catctgaaag
     1321 ttgtttcagt cgtttttctg aatcaataaa catgattttt tccctctgaa aatcccatgc
     1381 actaacgttg gttcaaatct aagccatcat gacacatctg tgcaacacct tgttgcgcgt
     1441 tgtcgcatcc aggatgcgac aacgttgtgt caccctggat gcgacaggta aataaaaaac
     1501 acact
//
LOCUS       DADLSE010000329         1478 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLSE010000329 DADLSE010000000
VERSION     DADLSE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.333"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            49..1029
                     /locus_tag="JN692_004336"
     CDS             49..1029
                     /locus_tag="JN692_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7266414.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWATALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          406 a          345 c          345 g          382 t
ORIGIN      
        1 taaaggggcc ctgctatcaa tttgtcattt gtattacgcg aaaattatat gaacgcaata
       61 atatcgcccg attattacta tgttcttacc gttgctggtc agtctaatgc catggcgtat
      121 ggcgaaggac tgccattacc ggacagggaa gatgcgcctc attccagaat taaacaatta
      181 gcgagatttg cgcatacgca tccaggaggc ccgccatgtc actttaacga cattattcca
      241 ctgactcact gcccacacga tgttcaggat atgcagggtt atcaccatcc tctggcaacg
      301 aatcatcaaa cacagtacgg caccgttggc caggcactgc atattgcacg gaaattactg
      361 cccttcattc ctgataatgc agggattctt atcgttccgt gttgccgtgg cggatcggct
      421 tttaccgcgg gcagcgaagg gacatattca gaacggcacg gagccagcca tgatgcttgc
      481 cgttggggaa cggatactcc gctgtaccag gatttagtca gcagaacgcg agccgcactg
      541 gcaaaaaatc cgcagaacaa attcctcggc gtatgctgga tgcaaggcga atttgactta
      601 atgaccagtg actacgcgtc acaccctcaa cactttaatc atatggttga agcctttcgt
      661 agggatctaa aacaatacca ttctcagctt aataatatta ctgacgcgcc gtggttttgc
      721 ggcgatacca cctggtactg gaaagaaaat ttcccgcatt cgtatgaagc tatttatggc
      781 aattatcaaa ataatgtttt agccaatatt attttcgtcg acttccagca acaaggtgaa
      841 agaggactga cgaacgcgcc tgatgaagac ccggacgatt taagcacggg atattacggt
      901 tcagcgtacc ggtcaccgga gaactgggcg acggcactgc gaagcagtca tttcagcacg
      961 gcagcccgtc gggggattat ttctgacaga tttgtagaag caattttgca gttttggcgc
     1021 gaaaggtgag cggggagttt attcatgggg gatgtccttg ttcgttctca gagtagacaa
     1081 caaaaaacaa atgagatcaa ggtaatatct cttcacttgc gggctcgaac agcaggcaat
     1141 tgccaatata ataatctggc acaagggtga aaaacgcgca ccgcataaac cgttgttatt
     1201 gcttacagat tagccggatg ccttaataga catccgcgcc ttttcgatta tggctcggaa
     1261 atctacgaac atctacacag tttactggaa cgttttgggc cacagcgttc gcagcaccgt
     1321 cctgatatgc cgttctggcg attataagtc gacggattct ggcaaataga cgatgatgat
     1381 atgcttaatg atgttcaaag aaattaggcc attattatat tattgcttgc attttctttc
     1441 attcctaaaa gcacccgcca ttaatcccag gcgcatta
//
LOCUS       DADLSE010000330         1434 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLSE010000330 DADLSE010000000
VERSION     DADLSE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.334"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..369
                     /locus_tag="JN692_004337"
     CDS             <1..369
                     /locus_tag="JN692_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266415.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            369..716
                     /gene="tnpB"
                     /locus_tag="JN692_004338"
     CDS             369..716
                     /gene="tnpB"
                     /locus_tag="JN692_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7266416.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWLVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            736..>1434
                     /locus_tag="JN692_004339"
     CDS             736..>1434
                     /locus_tag="JN692_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266417.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVAGKYADYLPLYRQSEIYRRQ
                     GVELSRATLGRWTGA"
BASE COUNT          341 a          401 c          394 g          298 t
ORIGIN      
        1 cctaattttc cttatgaatt taaaatcgcc ttagtggagc agtcactgca gcccggagcc
       61 tgtgtggcgc agatcgcccg tgaaaacgga atcaacgata acctgctctt caactggcgc
      121 catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgcctgc actgcttccc
      181 gtgacgttaa cgccggagcc ggataataaa atcccggctc cggcacagga accagagcag
      241 ataaatacac cgtccgacag tctgtgctgt gagctggttc tgccggccgg aactctcagg
      301 cttaaaggta aactgacgcc ggcgttatta cagatactta tccgcgaaat aaaagggagc
      361 agccactgat gatatctttc cctgcaggtt cgcgtatctg gctggttgca ggtatcaccg
      421 atatgcgaaa tggctttaac ggcctggcct caaaagttca aaacgtcctg aaggatgacc
      481 cgttctccgg gcatctgttc atcttccgcg gacgccgggg tgaccagata aaagtgttgt
      541 gggctgacag tgacggactg tgcctcttta ccagacgcct ggagcggggc cgcttcgtct
      601 ggctggtcac ccgtgacggc aaggtgcacc ttactccggc ccagttatcc atgcttcttg
      661 agggcatcga ctggaaacac ccgaaacgaa cggaacgcgc tggcatccgc atataacccg
      721 ttgtaaagtg aggatatgga cacctcactt gctcatgaga atgcccgcct gcgggcactg
      781 ttgcagacgc aacaggacac catccgccag atggcgaaat acaaccgcct gctctcacag
      841 cgggtggcgg cttatgcttc cgaaatcaac cggctgaagg cgctggttgc gaaactgcag
      901 cgcatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagatactg
      961 gaagcacagg agcgaatcag cgcacttcag gaagaaatgg cggaaacgct gggtgagcaa
     1021 tatgacccgg tactgccatc tcccctgcgc cagtcttcag cccgtaaacc gttacctgcc
     1081 tcacttcccc gtgaaacccg ggtcatccgt ccggaagagg aatgctgccc ggcctgtggt
     1141 ggcgaactca gttctctggg atgtgatgtg tcagagcagc tggagcttat cagcagcgcc
     1201 tttaaggtta tcgaaacaca acgtccgaaa ctggcctgtt gccggtgtga ccatatcgtg
     1261 caggcaccgg tgccttcaaa acccattgca cgcagttatg ccggagcggg gcttctggcc
     1321 catgttgtcg ctggaaaata tgcagactat ctgccgttat accgccagtc agaaatatac
     1381 cgtcgtcagg gcgtggagct gagccgtgcc acactggggc gctggacagg tgct
//
LOCUS       DADLSE010000331         1421 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLSE010000331 DADLSE010000000
VERSION     DADLSE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.335"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..186)
                     /gene="fimA"
                     /locus_tag="JN692_004340"
     CDS             complement(<1..186)
                     /gene="fimA"
                     /locus_tag="JN692_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7266418.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRT"
     gene            complement(667..1263)
                     /gene="fimE"
                     /locus_tag="JN692_004341"
     CDS             complement(667..1263)
                     /gene="fimE"
                     /locus_tag="JN692_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7266419.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          425 a          305 c          275 g          416 t
ORIGIN      
        1 ggtacgaacc tgtcccaact gaacagtttg atcaacagag cctgcatcaa ctgcgcaagc
       61 ggcgttaaca acttcccctt taaagtgaat ggtcccacca tttaccgtcg tagtatcggc
      121 cagagccgct gcggaactga gggacagagc cgacagaaca acgattgcca gagttttaat
      181 tttcatgctg ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta
      241 cactttgttt tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga
      301 aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta
      361 tttgttggct taatattcta ttattatctt tatttataga tttttatatt gcatgaggtg
      421 gtttttggag agaagaatga ggaagatgcg tcgagccaca gaaacgttag ctttacatat
      481 agcggaggtg atgtgaaatt aatttacaag agaaataatt tacatatcaa atagttagat
      541 gctttttgtc gttttttaat atttttatgc ttgagaaaaa atacgtaact tatttatgat
      601 atggacagtt tggccccaat tgtcttgtgt ttatttgttt tttaacttta ttatcaataa
      661 gttaaatcaa acctcttctc tttttaattt ttcgtttatg agattatttc tttcccataa
      721 tccggcaaaa cgagcagcat tactggcggt ataacgcaca gtatggcgaa tatttcgatg
      781 cccgagataa tcctgaatta aacgggtatc tgcaccacgc tccgccagtt cataaccgca
      841 agcatgcctt aacatatgag gatgagtctg cgttacggtt ccagcttcaa taccggcatc
      901 gcgaataatg cgataggcct gctggcgaga aagccgactc ccgcggcgag aaataaatat
      961 agcgtcggtc cggtcagcgc ctttccagtt agcacgttcc tgggtccagc gttccacggc
     1021 ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat
     1081 atttattcga ccttcattaa ggtcaaggtc ctgataatgc agatcaagca gttcactaat
     1141 acgcatccca tgccgatatg ccaacagaat aagacaataa tctctggctc ccgttgcccc
     1201 gtaacaaacc gcctgcatca tggcctgaac ttctttaccg gtaagataac gacgtttact
     1261 cacaatagta gtactcctaa ctgagatata tttgaatgaa tacctatagg aaacctcaat
     1321 cggtcaaaat tagcccaata gataaaaaga taaccacaca cgaagttatg tggctatcag
     1381 tcaattacat taaacatcat acaaaaataa aatatcactg a
//
LOCUS       DADLSE010000332         1398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLSE010000332 DADLSE010000000
VERSION     DADLSE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.336"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            43..630
                     /locus_tag="JN692_004342"
     CDS             43..630
                     /locus_tag="JN692_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266420.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(692..>1398)
                     /locus_tag="JN692_004343"
     CDS             complement(692..>1398)
                     /locus_tag="JN692_004343"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7266421.1"
                     /translation="EDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMYEYLTTL
                     VKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLTIDNYRE
                     HDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGG
                     DFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPS
                     LMVIADKAAAAELAFG"
BASE COUNT          359 a          324 c          331 g          384 t
ORIGIN      
        1 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
       61 ccgttacccc aattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat
      121 aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt
      181 tattgcgttg atgaagttga acatctctgt ccgttgtgta ttgcggacgg ttctgctgct
      241 gaaaagtttg caggtagttt tcaggatgat gccagcatag aaggtgttga atttgagtat
      301 gatgaagagg acgaatttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag
      361 ttggttgaac gcacgccagg ttatcatgga tggcaacagg aattctggct tgcgcattgt
      421 ggcgatttct gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat
      481 gaatttgcca accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct
      541 aaatgcctgc aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc
      601 aagctgagac tgtggggtga tttttcgtag ttatttaaat aatgagaaca ggccggagcg
      661 taattcatac atccggcctt atttcttcag cttaaccgaa cgctaattct gcggcagcgg
      721 ctttatcagc gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt
      781 cggtaaccgg accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa
      841 ttatcagtaa gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag
      901 gcactaacga aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct
      961 ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa
     1021 aatgaccatc tgcacctaat cccagcacca ccaaatccag gccgccttca cgcgccagtt
     1081 tctgatcatg ctcgcggtag ttatcaatag tgagcttctg gatattctct tctttgatcc
     1141 ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt tacgccttct ccctctttgc
     1201 cgcgaaaggg aatttcatcg aaattataga aatagcagtt atcgtaccaa ggcttacctt
     1261 taaccagggt ggtgaggtat tcatacatgc ctttgggcgt gctaccggcg gtaattgcca
     1321 ggttaacacg acgcgtcttc gacatataac caagcagatg gtgtgccgcg acacggctca
     1381 tttcctggta atcttcgg
//
LOCUS       DADLSE010000333         1382 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLSE010000333 DADLSE010000000
VERSION     DADLSE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.337"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..212
                     /locus_tag="JN692_004344"
     CDS             <1..212
                     /locus_tag="JN692_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HAY7266422.1"
                     /translation="GHTVLNPAVLPDGLTQPQYMDICMAMIRCVDAIYMLKGWQRSAG
                     AKAELALAEKLGHAVIFQETASVQD"
     gene            199..654
                     /locus_tag="JN692_004345"
     CDS             199..654
                     /locus_tag="JN692_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7266423.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDVLTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
     gene            752..1018
                     /locus_tag="JN692_004346"
     CDS             752..1018
                     /locus_tag="JN692_004346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266424.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALASLE
                     AEPVAWKAIFTQIDHEYNTFTALYSDKAEAERWVRLHKVGDFRA"
     gene            1141..1272
                     /locus_tag="JN692_004347"
                     /pseudo
     CDS             1141..1272
                     /locus_tag="JN692_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          401 a          275 c          376 g          330 t
ORIGIN      
        1 aagggcatac cgtcttaaac ccggcagtac ttccggacgg gctgacacag ccgcagtaca
       61 tggatatctg catggcgatg attcgttgtg tggatgcgat ttacatgctg aaaggctggc
      121 agcggtcagc aggcgctaag gcagaactgg cgctggcgga gaaactgggg catgcggtga
      181 tttttcagga gacagccagt gtgcaagact aattaccagg cattacgtga acgttattca
      241 ccaattcagg tgccggaatg tcctgtttgt ggcgatgaaa tgtcgataca gcgcatattt
      301 tccagaacgc atattgtgta tgcctgcaca ggtgaggggg atgatggata ttttaaaact
      361 ggtcgaactt ttgcggatga gcattacctg aaatcgcgcg taaccgtcgt tgatgttagc
      421 gatcctgacg tactggcttt actgaaagag ctggaagtta aagacaaacg cattgcagaa
      481 ttgacagatg tgcttacaca aatgattaat gcgcacaaaa ccacaattcg ttttggtcat
      541 gaacgcataa ctgaatgtgg tggtgattgc gactcgccgg aaaagatgat ttcagaaaat
      601 ccggatatca gaatggcaga ggctgttttg agagcaggaa taaaaactga ataattaaat
      661 ttagcactgt aaataaaatt taatccttaa ccggagggat ttctgcaccc tcagaacatc
      721 aggaggccgc ccgaaatggc ggtaatgaaa aatgactgaa ttaacaaaag agcaattaat
      781 cgaagaagct aaattaaaaa tagcgattgc gaaatgccat cccaattcag gcgtggcacg
      841 agtagagggt gagttattca aaattgcact ggcatcgctg gaagctgagc cggtggcgtg
      901 gaaggcaatc ttcacgcaaa ttgaccatga atataatacg ttcaccgctc tgtattctga
      961 caaagcagaa gcagaacggt gggtgcgact gcataaagta ggtgattttc gggcataaca
     1021 ccactttatg cagcatcgcc agcgctggta gtgcctgata agttgccgcg tgaatacata
     1081 agaggttggc ctcttgcgta tagtgattat gctgaaggat ggaacgattg tcgtgcagcc
     1141 atgcttcagg gatggcagcc atgccatcaa attaccgcct caatggcaaa agccattgcg
     1201 cttaaacttg gtgctgaact gaataacgaa gaagcagaga ttttcgcgga tgggtataac
     1261 gccgccatgc ttcatgccgg aaactgaacc gtagcaggag gtgaatcgat gagctggcct
     1321 gaagcattca caacggtagg aattgcaatg gcggtggcgc tggttgtgta ttcgatttgc
     1381 cg
//
LOCUS       DADLSE010000334         1378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLSE010000334 DADLSE010000000
VERSION     DADLSE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.338"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            7..807
                     /locus_tag="JN692_004348"
     CDS             7..807
                     /locus_tag="JN692_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7266425.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIAHDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTVRTTGRAEATIL
                     VDIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            1004..>1378
                     /locus_tag="JN692_004349"
     CDS             1004..>1378
                     /locus_tag="JN692_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HAY7266426.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGE"
BASE COUNT          341 a          342 c          347 g          348 t
ORIGIN      
        1 ggaaccatga acattacccc gtttccgacg ctttcgacgg caactataga tgccataaat
       61 gttatcggac agtggctggc gcaggatgat ttctccggtg aggtgccgta tcaggctgat
      121 tgcgtcatac ttgcaggaaa tgcggttatg ccgactatcg atgcggcatg taagattgct
      181 cacgatcagc aaattccttt actgattagt ggtggtatcg gtcactcgac cacttttttg
      241 tatagcgcca tcgcacagca tccgcactac aacactgtcc gcaccactgg cagagcagaa
      301 gcgaccatcc tggtggatat cgctcatcag ttctggcata ttccgcatga aaaaatctgg
      361 attgaagacc agtcaacaaa ctgcggtgaa aacgcacgct ttagcatcgc gctattgaat
      421 caggccgtag aacgagttca tacggctatc gttgtacagg accccaccat gcagcggcgc
      481 acgatggcaa cgttccgccg tatgaccggg gacaatcccg atgcaccacg ctggttaagt
      541 tatcccggat tcgttcctca gttaggaaat aacgcagaca gtgtaatctt tattaatcag
      601 ttacaaggat tatggccggt tgagcgttat ctctcactac tcactggcga gctgccgcgt
      661 ttacacgatg atagcgatgg ctacggtccc cgcgggcgag attttatcgt tcacgttgat
      721 tttccggcag aagtcatcca tgcatggcaa acgctgaaac atgatgcggt gctcatcgag
      781 gcgatggaaa gtcgctcgtt acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa
      841 cgggcatgac tcctgacttt tatttctgcc ttttattcct tttacacttg tttttatgaa
      901 gccctccaca gaattgtcct ttcccgatgc cgtctctctg atgattgatg ttaattaaca
      961 atatattcac cgaaaacaaa catataaatc acaggagtcg cccatgtcag tacccgttca
     1021 acatcctatg tatatcgatg gacagtttgt tacctggcgt ggagacgcat ggattgatgt
     1081 ggtaaaccct gctacagagg ctgtcatttc ccgcataccc gatggtcagg ccgaggatgc
     1141 ccgtaaggca atcgatgcag cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat
     1201 tgaacgcgcc agttggttgc gcaaaatctc cgccgggatc cgcgaacgcg ccagtgaaat
     1261 cagtgcgctg attgttgaag aagggggcaa gatccagcag ctggctgaag tcgaagtggc
     1321 ttttactgcc gactatatcg attacatggc ggagtgggca cggcgttacg agggcgag
//
LOCUS       DADLSE010000335         1358 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLSE010000335 DADLSE010000000
VERSION     DADLSE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.339"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            210..998
                     /gene="virF"
                     /locus_tag="JN692_004350"
     CDS             210..998
                     /gene="virF"
                     /locus_tag="JN692_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7266427.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(940..1261)
                     /locus_tag="JN692_004351"
                     /pseudo
     CDS             complement(940..1261)
                     /locus_tag="JN692_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741395.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          443 a          230 c          247 g          438 t
ORIGIN      
        1 gtccctttca gtgcaaatac ttagcttgtt gcacagagaa atagaagctg cataagctct
       61 ttcttcaaaa aatgtaaata aagttaaata taggaaaaat tacttaatct atcttaataa
      121 cggaaagatt ttgtatacaa tcactgttac agaaatttct tagttactct gtaaacacta
      181 aatatagttt ggtatattct gttgaattta tgatggatat gggacataaa aacaaaatag
      241 atataaaggt tcgcttgcat aactatatta ttttatatgc aaaaaggtgt tcaatgacgg
      301 ttagctcagg caatgaaact ttgactatcg atgaagggca aattgctttt atagagcgaa
      361 atatacaaat aaacgtctcc ataaaaaaat ctgatagcat taatccattt gagattataa
      421 gccttgacag aaatttatta ttaagcatta ttagaataat ggaaccaatt tattcatttc
      481 aacactccta ttctgaggag aaaagggggt taaacaaaaa aatattcctc ctctctgagg
      541 aggaggtttc tatcgatttg ttcaaatcta taaaagagat gcctttcggc aaaagaaaga
      601 tctatagttt agcttgcctt ttatcagctg tttctgatga ggaagcttta tatacttcga
      661 tatcgatagc ttcttctctt agtttttctg atcagataag gaagattgtt gaaaaaaaca
      721 tcgagaagag atggcgtctt tctgatattt caaataactt gaatttatca gaaatagctg
      781 ttagaaaacg attggagagt gaaaaattaa catttcaaca aatccttctt gatattcgca
      841 tgcatcatgc agcaaagctt ttattgaata gtcaaagcta tattaatgat gtatcaagac
      901 ttatcggaat atcaagccca tcttatttta taaggaaatt taatgaatat tatggtataa
      961 ctccaaagaa attttactta tatcataaaa aattttaaat gcttcatagc ccatcgctat
     1021 tgccagatgg gttttcccca cacctgatgg ccccagtaat acgatatttt cattacgttc
     1081 tatgaagctg agtgagcgta acgactggag ttgcttctgc ggtgctccgg tggcgaatgt
     1141 gaagtcatac tcttcgaacg ttttcaccgc cgggaaggct gccattcggg tatacatcgc
     1201 ctgtttacgt tgatgacgtg ccagtttttc ttcatgaagc atgatgctcc aggaagtcca
     1261 tataactcca ttcctggtct actgcctgtt gtgacagcgc aggcgctgcg cttatgtact
     1321 gcacccattt tgttggacga tgaaatggaa tagcccct
//
LOCUS       DADLSE010000336         1347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLSE010000336 DADLSE010000000
VERSION     DADLSE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.340"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            443..1192
                     /locus_tag="JN692_004352"
     CDS             443..1192
                     /locus_tag="JN692_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7266428.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          376 a          275 c          280 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtcaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctgggtgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcatctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aatttca
//
LOCUS       DADLSE010000337         1331 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLSE010000337 DADLSE010000000
VERSION     DADLSE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.341"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..338)
                     /locus_tag="JN692_004353"
     CDS             complement(<1..338)
                     /locus_tag="JN692_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7266429.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFA"
     gene            420..647
                     /gene="vapB"
                     /locus_tag="JN692_004354"
     CDS             420..647
                     /gene="vapB"
                     /locus_tag="JN692_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7266430.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            647..1045
                     /gene="vapC"
                     /locus_tag="JN692_004355"
     CDS             647..1045
                     /gene="vapC"
                     /locus_tag="JN692_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7266431.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1054..>1331)
                     /locus_tag="JN692_004356"
     CDS             complement(1054..>1331)
                     /locus_tag="JN692_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7266432.1"
                     /translation="SPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT          324 a          377 c          324 g          306 t
ORIGIN      
        1 gcaaaatcca cggcctggtt atgtgcttca ataagacgtt tgtccccccc gagcatggcc
       61 atcatggaga cacttttggg agcggagaag gtcaggtcgt agccgggacg atgcctgtta
      121 ctgccatcct gcatgcggct taaatccgcc ccgtccggca gcctgccctc cagaaggcgg
      181 gtaaagacat ccttatcgac actgccctgc agccccagtt gttcagcccc ctggccggcc
      241 cagcgttctc ccatgctgcc cagcacatag taattatcct tgtcggtata ataattaccg
      301 gcacttccgg ccgatctgac ctgcgcgata ctcatcataa acgtatatcc ctttgacata
      361 tcccggtatc aatcccacaa tagatataca caagacatat ccacataagg aggcaaataa
      421 tggaaaccac cgtatttctc agcaaccgca gccaggcggt cagactgcca aaagcggttg
      481 cattgccgga aaacgtaaaa cgcgttgaag tgattgctgt cggacgaacc agaatcatta
      541 cgccagccgg agagacatgg gacgaatggt tcgacggaca cagcgtcagc gccgatttta
      601 tggataacag ggaacagccc ggcatgcagg aaagggagtc attctgatgc tgaagtttat
      661 gctcgatacc aacatctgca tttttacgat aaagaacaaa cccgccagcg tcagggagcg
      721 ttttaacctg aaccagggga gaatgtgtat cagttcggtc accctgatgg agctgatata
      781 tggtgcagaa aaaagccaga tgcctgaacg taatctcgct gtgatcgagg gatttgtttc
      841 ccgcattgat gttctggatt acgacgctgc tgcagccaca cacaccggcc agataagagc
      901 agaacttgcc cgtcaaggac gccctgtcgg gccatttgat caaatgatcg caggtcatgc
      961 ccgcagtcgg gggctgatta ttgtgactaa taacacccgg gaatttgaac gtgtgggcgg
     1021 cctgagaact gaagactgga gctgacctgc tcatcagaaa tcatctcccg gctcaacatc
     1081 ctccccgcgt tcccggtgca cattgatgtt cacctcttca cgacgctgca tctgctgcca
     1141 ggtgtccgga tgattttcct gctgccatgc ctcataagcg gccatatcca ccacttcacc
     1201 ggattcactg atcccgggtg gcagttgctg ttccatctct tcttccggtt tcattttcag
     1261 ctcctgctcg atcccccctg ccggaacatt cacacctgaa tctgacttct tatcgttgat
     1321 gacaggagac a
//
LOCUS       DADLSE010000338         1281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLSE010000338 DADLSE010000000
VERSION     DADLSE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.342"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..1019
                     /locus_tag="JN692_004357"
     CDS             <1..1019
                     /locus_tag="JN692_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7266433.1"
                     /translation="FSCTINYVQSLRELYTDVIEEFYWVALPLTTQNSLSQYQPEWQC
                     WEPDVEWVRQPPQDAITAPDFFCFYQPGMTFEQFVREFAEWFSQKRPAAMMIGIRADE
                     SYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTWYANHQNLCNP
                     LYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVSGVKSGGIYAG
                     HDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQ
                     TQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGIL
                     CNND"
     gene            992..>1281
                     /locus_tag="JN692_004358"
     CDS             992..>1281
                     /locus_tag="JN692_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7266434.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK"
BASE COUNT          371 a          313 c          291 g          306 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct ggtatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaagccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag tggattgcgt
     1141 tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca acgtggcacc
     1201 acccgaaaaa aaactgctgc aaaaatccat tgaaatagat ggttttaccc aacccattgt
     1261 ggtcacacat acggataaaa a
//
LOCUS       DADLSE010000339         1261 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLSE010000339 DADLSE010000000
VERSION     DADLSE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.343"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..84
                     /locus_tag="JN692_004359"
     CDS             <1..84
                     /locus_tag="JN692_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266435.1"
                     /translation="RDELIDTTQARPIQYCINGTWYNAGSI"
     gene            87..620
                     /locus_tag="JN692_004360"
     CDS             87..620
                     /locus_tag="JN692_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7266436.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQMENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(649..1176)
                     /locus_tag="JN692_004361"
     CDS             complement(649..1176)
                     /locus_tag="JN692_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7266437.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEERARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1178..>1261)
                     /locus_tag="JN692_004362"
     CDS             complement(1178..>1261)
                     /locus_tag="JN692_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266438.1"
                     /translation="SADNIAGVYYRPIQKYINGTWYNVASV"
BASE COUNT          360 a          282 c          276 g          343 t
ORIGIN      
        1 agagatgaac taattgatac aactcaggca aggccaattc agtattgcat taatgggacg
       61 tggtataacg cggggagtat ttaacgatga tgcacttaaa aaacatttct gctggtaatc
      121 ctgaaacaaa agagcaatac cagctaacga aacaatttaa catcaaatgg ctttatacag
      181 aggatgggaa aaactggtat gaggaacaaa agaactttca gcctgatacg ttgaaaatgg
      241 tctatgacca caacggcgtt attatttgta ttgaaaagga tgtttcagca attaatccgg
      301 aaggcgcaag cgtcgttgag gttcctgata ttacagcaaa tcgccgggct gatatttcag
      361 gtaaatggat gttcaaagat ggcatagtga taaagcgaac ttataccggg gaagagcaga
      421 ggcaacaaat ggaaaatgaa aagcaaagcc tgttgcaact tgtcagggat aaaacccagc
      481 tatgggactc acagctacgg ctgggcatca tttccgccgg gaataagcag aaattaaccg
      541 agtggatgct ctacgcgcag aaggtcgaat ccacagacac ctccagtctg ccagtaacgt
      601 ttcccgaaca accagaatga gagaaggccc gctatcgggc cttaattttt actctggttt
      661 ttgtggccat tctggctttg ccgtatccac acggctgacc agaacactgt agcgttccca
      721 tgactccagt cgtgcgcgtt cctcatctgt tgccatattc agcctgacag agcgctccag
      781 cggctggatg actgattcag cttcggaaag caatgcggct ttttgtgatt cggcctgttg
      841 ctgctgttcg tctgccgtat aaatccgctt aaccacagct ccgtccttaa acatccactt
      901 cccggaatca tcggcacgac gatttgctgt tatatcgggg acctcaacga cgctaaagcc
      961 ttcaggatta agcgtggagg catctttagt gatggcgaca ataatattat tttcatcgta
     1021 aacaatcttt attgtatctg gctgaaagtt cttcacttcc ccataccagt tttttccgtc
     1081 ttctgtccac aaccagatta cgtcaaaatt ctttgttaat tcgtactgtt ccagtgtttt
     1141 agcgttaccc gctttaatgt tctttaagtg catcatatta aacgctcgct acattatacc
     1201 aggtgccatt tatatacttt tgaatcggtc tgtaataaac acccgcaata ttatcggcag
     1261 a
//
LOCUS       DADLSE010000340         1260 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLSE010000340 DADLSE010000000
VERSION     DADLSE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.344"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>1260
                     /locus_tag="JN692_004363"
     rRNA            <1..>1260
                     /locus_tag="JN692_004363"
                     /product="23S ribosomal RNA"
BASE COUNT          318 a          282 c          391 g          269 t
ORIGIN      
        1 aagcatcagg taacatcaaa tcgtacccca aaccgacaca ggtggtcagg tagagaatac
       61 caaggcgctt gagagaactc gggtgaagga actaggcaaa atggtgccgt aacttcggga
      121 gaaggcacgc tgatacgtag gtgaagcgac ttgctcgtgg agctgaaatc agtcgaagat
      181 accagctggc tgcaactgtt tattaaaaac acagcactgt gcaaacacga aagtggacgt
      241 atacggtgtg acgcctgccc ggtgccggaa ggttaattga tggggttagc gcaagcgaag
      301 ctcttgatcg aagccccggt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      361 ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa tgatggccag gctgtctcca
      421 cccgagactc agtgaaattg aactcgctgt gaagatgcag tgtacccgcg gcaagacgga
      481 aagaccccgt gaacctttac tatagcttga cactgaacat tgagccttga tgtgtaggat
      541 aggtgggagg ctttgaagtg tggacgccag tctgcatgga gccgaccttg aaataccacc
      601 ctttaatgtt tgatgttcta acgtggaccc gtgatccggg ttgcggacag tgtctggtgg
      661 gtagtttgac tggggcggtc tcctcctaaa gagtaacgga ggagcacgaa ggttggctaa
      721 tcctggtcgg acatcaggag gttagtgcaa tggcataagc cagcttgact gcgagcgtga
      781 cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt ggttctgaat ggaagggcca
      841 tcgctcaacg gataaaaggt actccgggga taacaggctg ataccgccca agagttcata
      901 tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca tcctggggct gaagtaggtc
      961 ccaagggtat ggctgttcgc catttaaagt ggtacgcgag ctgggtttag aacgtcgtga
     1021 gacagttcgg tccctatctg ccgtgggcgc tggagaactg aggggggctg ctcctagtac
     1081 gagaggaccg gagtggacgc atcactggtg ttcgggttgt catgccaatg gcactgcccg
     1141 gtagctaaat gcggaagaga taagtgctga aagcatctaa gcacgaaact tgccccgaga
     1201 tgagttctcc ctgacccttt aagggtcctg aaggaacgtt gaagacgacg acgttgatag
//
LOCUS       DADLSE010000341         1242 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLSE010000341 DADLSE010000000
VERSION     DADLSE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.345"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            28..>1242
                     /locus_tag="JN692_004364"
     CDS             join(28..352,352..>1242)
                     /locus_tag="JN692_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HAY7266439.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAKKLDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNR"
BASE COUNT          308 a          296 c          376 g          262 t
ORIGIN      
        1 gtttttagac tagtcattgg agaacagatg attgatgtct tagggccgga gaaacgcaga
       61 cggcgtacca cacaggaaaa gatcgcaatt gttcagcaga gctttgaacc ggggatgacg
      121 gtctccctcg ttgcccggca acatggtgta gcagccagcc agttatttct ctggcgtaag
      181 caataccagg aaggaagtct tactgctgtg gccgccggag aacaggttgt tcctgcctct
      241 gaacttgctg ccgccatgaa gcagattaaa gaactccagc gcctgctcgg caagaaaacg
      301 atggaaaatg aactcctcaa agaagccgtt gaatatggac gggcaaaaaa gtggatagcg
      361 cacgcgccct tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt
      421 cgcgtgcgca gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca
      481 gtcgtcacac tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggagagctgc
      541 ccacgtatgg ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta
      601 tgcctgcgat caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg
      661 agcgaaaacc tgctgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag
      721 aaagcaatca gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac
      781 tgcgtgtcac gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtgacta
      841 ccggcggctt caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct
      901 tcggcaacga tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc
      961 gggctaatga aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg
     1021 tgcggagtcc ggagagtaac ggaatagcag agagcttcgt gaaaacgata aagcgtgact
     1081 acatcagtat catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt
     1141 tcgagcatta taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat
     1201 atctgcggca gcgggcttgt aatgggttaa gtgataacag at
//
LOCUS       DADLSE010000342         1241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLSE010000342 DADLSE010000000
VERSION     DADLSE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.346"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN692_004365"
     CDS             complement(<1..689)
                     /gene="ipaH7.8"
                     /locus_tag="JN692_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7266440.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
     gene            1019..1198
                     /locus_tag="JN692_004366"
                     /pseudo
     CDS             1019..1198
                     /locus_tag="JN692_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          405 a          179 c          247 g          410 t
ORIGIN      
        1 ttaatcctgt aacttgaatc taattgtgaa aaactctccg gaagagtggt tatctgatta
       61 aaatgaaaat aatattcctt ttgtctgata ttgtttctat cagaaaattg aggaaaagta
      121 accagattat tgcgctgagc acagagaatt tccagtttat ccggtaagtc gggtaatgtt
      181 gtcagcctgt tataataaac atgtaaaaat ttcaattttt cgggaagagc cggtaagtta
      241 tatagcctgt tattttcaac aaataatgat gttaaatggt ttggcaaaac aggaaggttt
      301 tccagttgat tagaacgaac acttaaagtt gttaatgact caggaagttc tggtatcaca
      361 gaaagcctgt tattatcagc attaagttct ttgagaaatg caggcagctt tggcaatgat
      421 gttaatttat tattttctac aatcaaagca gaaatatgct ttggtaattc gggaagagac
      481 gtcaaattta ggtctgataa atttaatact gcttcttgat tctgaaagca ctgggagagt
      541 ctgttaaaag caatgcctcg ctcttccccg ggagaagagt tcttttccca ttcagtcagg
      601 attctcagat aatgttcatt actggttgag tttgagttaa tagaggggga gcaagaaact
      661 gatgagtgtg tattatttac agagaacata atgctaggtc aaccttatat ttgtgagaat
      721 aaatatttaa aattgtatat ttatacaaat ttcacttacc atttccgatg ttaagtttta
      781 atagtttcca ttttacacat aacagaagtt atgttgtaat ttatttattg aggttttcta
      841 tggaattctt tttttattgt gactgcagtc aataataaca ctgaattcaa cattccagtg
      901 agcaaggaaa tgtaatccgg gtaagtatat ttgttttaca tcatatttta gccaagctca
      961 tttattccta ttatagaatg ataaattaaa attactgtaa aaaaacgata cacatttaat
     1021 acgagggagg ccggcagaaa aacggcttgc ggtaaggcag gagaaatgtg tgccgcgaat
     1081 gaagtccttg tatgactgga taaagctcca gaggaaaact ttgtcaaacc atgctgaaat
     1141 ggtaaaggcg ttcaactata tcctgaatca ctggaatgca ctgaatgagt tttgccgggt
     1201 agtacttcca atcattagaa tatgtgcttt tcaatgaagg a
//
LOCUS       DADLSE010000343         1232 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLSE010000343 DADLSE010000000
VERSION     DADLSE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.347"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(41..>1232)
                     /locus_tag="JN692_004367"
     CDS             complement(41..>1232)
                     /locus_tag="JN692_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7266441.1"
                     /translation="FVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLC
                     PQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLG
                     GASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAKGKGLA
                     YEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIEAARFG
                     KSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYI
                     RTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEY
                     MGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
                     LLNW"
BASE COUNT          325 a          337 c          282 g          288 t
ORIGIN      
        1 ctaaatcagt aagttggcag cattaccaag cgaaccgtta ttaccagttc aacaatgtgc
       61 catcttccag tcgtgcgacg gggagatacg ccgggtcata cggatatttc gccgccagtt
      121 tctcatcgaa agaaataccc agccccggtt catcgcctgg gtgcatataa ccttcctcga
      181 agcgccagct gtgcgggaaa acttcaagca tttgttcgga atagcccatg tactcctgaa
      241 cgccaaaatt tggtacccat aggtcaaaat gcagcgccgc cgcatgacaa acaggcgata
      301 aatctgaagg gccatgcgaa ccggtacgta cctgataaag cgatgcaaaa tcggcaatcc
      361 gccgcatccc ggtaatgccg cctgcatggg tgatcgtcgt gcgaatatag tcaatcagct
      421 gctcttcgat taactgcttg cagtcccaga tactgttaaa cacttcaccg accgcaatcg
      481 gcgtcacggt atgctggcga atcagacgaa aacattcctg gttctcggca ggcgtcggat
      541 cttccatcca gaacaggcgg taatcctcaa tgctcttccc aaagcgcgcc gcttcgatgg
      601 gggtcaggcg atggtgcatg tcatgcaaca gatgttcgtt aaaaccgaac ttattacgca
      661 ccgcttcgaa taacttcggc gtaaaatcga gatacttttc tgaagaccac aactgctctt
      721 ctggccacag gcctttggtt gccggttcat acgccagtcc tttgcccttc gccaggccat
      781 aagtggtttg catacccggt acaccgcact gcacacgaat cgccttaaag cccatctgct
      841 ggtgctttgc atagtcttca aggacttcat caatcgtacg cccggtggtg tggcaataaa
      901 ccatcacgcc ttcacgagaa gcaccaccaa gcagttgata caatggcata tttgccgctt
      961 tagctttgat atcccacaac gccatatcaa tggcagaaat ggctgacatc gtgacggggc
     1021 cacgtcgcca gtaagcacct ttataaaaga attgccagat atcttcgata cggctggcat
     1081 cgcggccaat tagttgtgga caaagatgat ctttcagata agaagcgacc gacaattcgc
     1141 gaccatttaa cgtggcatct cctaatccac aaagtccgct ttcagtggtt attttaagcg
     1201 taacgaaatt acgccctggg caagtaacaa aa
//
LOCUS       DADLSE010000344         1228 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLSE010000344 DADLSE010000000
VERSION     DADLSE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.348"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..131
                     /locus_tag="JN692_004368"
     CDS             <1..131
                     /locus_tag="JN692_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7266442.1"
                     /translation="DREKLKAYRIYAKSLQAMDFSVITDKTSYNAIEWPASPEISS"
     gene            155..>1228
                     /locus_tag="JN692_004369"
     CDS             155..>1228
                     /locus_tag="JN692_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266443.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWHNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIG
                     GLIIAAYQGEADGDKVIRLVRGQS"
BASE COUNT          325 a          300 c          341 g          262 t
ORIGIN      
        1 aagaccggga aaaactgaaa gcgtaccgca tttacgcgaa atcgctgcag gcgatggatt
       61 tcagtgtcat cactgataaa acctcataca acgccattga atggcccgct tctccggaaa
      121 tttcttcctg atttaattta tcgcgagaaa aactatgtct gtagtgatat caggtgcgct
      181 gactgatggc gcaggtatcc ccatgtccgg ataccatatt attctgaaat cccgggtaaa
      241 caccccggaa gtggtgatgc acactgttgc tgatgtgatg acaggaaacg atggtgaata
      301 ctgtttccat gcgcggactg gaaaatatgg tgtgtatctg aaacaggact ggcacaacga
      361 gtacaacgtt ggcgacattg ctgtatatga ggactcaaag cccggcacgc tgaatgactt
      421 tctgattgct cctgatgagg gcgacctgaa accggatgtc gtcaaacgct ttgaggaaat
      481 ggtggcgcag gcgcagcaga gcgccggggc cgcagccgga aacgcacagc agacggcgca
      541 ggatgtggcg gcagccgcag gttatgcccg cgcagcagag caggccaaaa atgacattga
      601 tgctgcgctg accggcactc tgaaaacggc taaccatctg tctgaaatcg cagcagcagg
      661 cgaaaaggca caacagaagt cccgggataa tctggggctg aaaagtgcgg ccacgatgga
      721 agcacagagc gacatttacg accggacaaa aggccgtctg gcgatacccg gcgcattcgg
      781 ctttgggtgt gcttttctgc ctgaagatgt tatccgtttt gacactaaga gtgatttcct
      841 ggcctgggta aggaatgcgc tgccaggtga atattccgtt gctggcccct acggcatcat
      901 catacccgac acacggtttg aaggggtgct cagcatccgg tggactgatg cacgccctga
      961 gacaacagaa ccgaggtaca gagccaaatc ccttactttt tacggcatta acggccccat
     1021 ttatcacacc cgctactgct actggcccat atccagactg actggctggg tgaaaataaa
     1081 tataaccaca gaagatatta tttacagaat cgtggcgagc tctgtccgca acagatgggg
     1141 agaccctgac attggcgggc tgattattgc tgcgtaccag ggagaagctg acggtgataa
     1201 agtcatcaga cttgtcaggg ggcagtca
//
LOCUS       DADLSE010000345         1199 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLSE010000345 DADLSE010000000
VERSION     DADLSE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.349"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN692_004370"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="JN692_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7266444.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          214 c          259 g          385 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagtctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaataatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DADLSE010000346         1192 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLSE010000346 DADLSE010000000
VERSION     DADLSE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.350"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(40..549)
                     /locus_tag="JN692_004371"
     CDS             complement(40..549)
                     /locus_tag="JN692_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7266445.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            678..1007
                     /locus_tag="JN692_004372"
     CDS             678..1007
                     /locus_tag="JN692_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7266446.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQELREELYQVKQVVKGMRDGM
                     FSVLNES"
BASE COUNT          349 a          249 c          309 g          285 t
ORIGIN      
        1 gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc ctgaatggcg
       61 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
      121 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcaccacg
      181 gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa tatgcgggcg
      241 aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
      301 aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat tttgccactg
      361 atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat gggccagaaa
      421 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
      481 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
      541 tagattcatt actgctcctg aacggtatac aggagtaagt gtagacgttg gtaaatgatg
      601 tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc gcaagcaact
      661 aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcacc atttccaaaa
      721 cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc aaaaccggta
      781 tagcccggta ttattcacaa catcccagcc tgcaccttaa gggcgactgg ctgaaagagg
      841 ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggtgc ctgaccatca
      901 tcgctgacag tgacgaggtg caggaactgc gggaggaact ttatcaggtt aaacaggtag
      961 ttaaggggat gcgggatggg atgtttagtg tgctgaacga aagttaaata ttaaaaaagc
     1021 tgggaagatt taaaaccttc ctagcttttt attctgtctt attataatgg atactcatca
     1081 tcttccgatg aatttataaa ttgctccaag tttacatgaa gagtacttaa cctagttttt
     1141 aactcattaa gtgaagcgat aatttcatat tttccaacat aatcatatga tg
//
LOCUS       DADLSE010000347         1160 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLSE010000347 DADLSE010000000
VERSION     DADLSE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.351"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(11..568)
                     /locus_tag="JN692_004373"
     CDS             complement(11..568)
                     /locus_tag="JN692_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7266447.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            732..>1160
                     /locus_tag="JN692_004374"
     CDS             732..>1160
                     /locus_tag="JN692_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HAY7266448.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPG"
BASE COUNT          267 a          315 c          269 g          309 t
ORIGIN      
        1 aataggcgta tcacgaggcc ctttcgtctt caagaatttt ataaaccgtg gagcgggcaa
       61 tactgagctg atgagcaatt tccgttgcac cagtgccctt ctgatgaagc gtcagcacga
      121 cgttcctgtc cacggtacgc ctgcggccaa atttgattcc tttcagcttt gcttcctgtc
      181 ggccctcatt cgtgcgttct aggatcctcc ggcgttcagc ctgtgccaca gccgacagga
      241 tggtgaccac catttgcccc atatcaccgt cggtactgat cccgtcatca atgaaccgga
      301 ctgccacgcc ctgagcgtca aattccttta tcagttggat catatcggca gtgtcgcggc
      361 caagacggtc gagcttctta accagaatga catcaccttc ctccaccttc atcctcagca
      421 aatccagccc ttcccggtct gttgaactgc cggatgcctt atcggtaaat atacggtttg
      481 ctttcacacc tgcgtctttg agtgctctga cctgaagatc aagagactgc tgactggttg
      541 agacccgagc gtaaccaaaa agtcgcataa aaatgtacct taaatcgaat atcggacaac
      601 tcatgtctat tattacaaat ttacgattta atagacatat taatgtaaca gttttacgat
      661 gtccgataat ttataatatt tcggacggtt gcgaaattgt taattcatga ccatcaggca
      721 gggaggccgg tatgcccgtc gattttctga ccactgagca ggaactgaat tatggtcgct
      781 atgttgcaga acccaatgac gtgcagctgg cgcgctattt tcatcttgat gagcgggatc
      841 ttgccttcat taaccagcgg cggggaaggc ataaccggct gggaattgcg cttcagctca
      901 ccaccgcccg ttttctgggc acctttctga cagacttaac ccgggttctg ccaggtgttc
      961 agcaatttgt cgcggtgcag cttaatattc gccgtccgga agtccttacc cgctacgcag
     1021 aacgggttac caccctcaga gagcatactg cgctgattaa ggaatattac ggttaccatg
     1081 aatttggtga tttcccctgg tctttccgcc tgaagcgcct gctgtacacc cgcgcatggc
     1141 tcagtaatga gcgacccggc
//
LOCUS       DADLSE010000348         1146 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLSE010000348 DADLSE010000000
VERSION     DADLSE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.352"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..748)
                     /locus_tag="JN692_004375"
     CDS             complement(<1..748)
                     /locus_tag="JN692_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266449.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPLVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVY"
     gene            complement(748..1113)
                     /locus_tag="JN692_004376"
     CDS             complement(748..1113)
                     /locus_tag="JN692_004376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266450.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
BASE COUNT          269 a          330 c          290 g          257 t
ORIGIN      
        1 agtacaccgc cacatgggtg gcattatcca tcacccggga tacgttgaca ttccagccac
       61 ggcggtaatg cgtgtccagg aagcgattcc attcccgttt actgcttcct tctgctgcca
      121 gcgcatccgg catcaccagg tcagggtatt tccgtgacag caaccgtgtt atccggtagc
      181 gccacatgct catcacctta cgggcgtaaa aatgaagatt tttccaggtg tggcccgacg
      241 tcacaccacc ggcagttgtc gataaatgga tatgcggatg ccactgctgg tcacgccccc
      301 atgtgtggat caccgtgaat atccccggct ccacatctgt ctgatggcag atttccagta
      361 tcacatccgc tgcaatgcgg ctcatctctg ccagtaacca ccggttgtgg aacaccaggg
      421 accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtcg gggaccaggc
      481 tcagcagata ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg caggagcggc
      541 ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa caacaccact
      601 gcgtgtagcc catcagtgtg gtcccgcagg ccatgatttt tgtcactgat tcaattacca
      661 acggacggac tgccccttcc ggttgtttct ccagccagtt aagccagcgg tttccctgct
      721 gaaaaatatc ggcaaaacgg ggaagcatca gaagggcggg gcgactccgt ccggccagtg
      781 aaccgtgcca cactccgggc agtacatacc gccggcgctg ataccggcaa gaatggtcgc
      841 aaattcccgc tccgtgcagc gggcgatttc cggataccct tcgtcatcaa cacgtacaaa
      901 ccagaagacc agctttttgt ttcccgcatc cacaaagaac ggaatattca ggtctgcgca
      961 gcattcaacg gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt cttcgtcaaa
     1021 aaaatcatct tcgtgaagct tcacgacata gcggggaagt ttgcttcttt gagaggcggg
     1081 tttacgttta cggggtttag ctgaacgggc catataacca cctgaaagac aatgacattg
     1141 cctgtt
//
LOCUS       DADLSE010000349         1145 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLSE010000349 DADLSE010000000
VERSION     DADLSE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.353"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(11..202)
                     /locus_tag="JN692_004377"
     CDS             complement(11..202)
                     /locus_tag="JN692_004377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266451.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            339..1145
                     /locus_tag="JN692_004378"
                     /pseudo
     CDS             339..1145
                     /locus_tag="JN692_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          302 a          296 c          330 g          217 t
ORIGIN      
        1 gtagtgtccg ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg
       61 cggcaaccaa tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt
      121 cggcagcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt
      181 cagcagcgaa gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg
      241 cgacggcggc agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag
      301 cagcaaaacg ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga
      361 aaaaggggat agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga
      421 cagcgaaagc ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc
      481 cggctctgac cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga
      541 tcgccagtac ggcttatgtt atggccgcga ttgctgccat cgtggactcg tcgcctgatg
      601 cactgaacac gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca
      661 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg
      721 ggcttgctac tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg
      781 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg
      841 aatacctcgg tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg
      901 ataccttgtc cggtggactt acttttgaaa acgactcaat ccttgcctgg attcggaata
      961 ctgactgggc gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt
     1021 ggtttgaaac ggcggataac ggtaatgaat atttcaaatg gagacacgcc tcgctggcgg
     1081 ccgggttaaa gacctgatga atctcaaatg ggatacacta aatattctgg ttaatgccgt
     1141 catta
//
LOCUS       DADLSE010000350         1144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLSE010000350 DADLSE010000000
VERSION     DADLSE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.354"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            293..1135
                     /locus_tag="JN692_004379"
     CDS             293..1135
                     /locus_tag="JN692_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266452.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
BASE COUNT          389 a          197 c          221 g          337 t
ORIGIN      
        1 ctgtagatcg ctgtgtgttg catctatcgg ttgaactcac agcccaaacc agactgtcag
       61 ctttgattag attctaccta ctcaacctgt cagataaggt ctgagctaat acatctaata
      121 tatggctttg tgcaggggac ggctatcgcg aacgttaggc taggtatctg aatacgcaca
      181 gttcacttgg ttgtacttct tgcactgatc tgctatcccg cacagctatt tataggtaat
      241 cttcagtgta taatctgccg acttatggaa gcttgtacaa caggagaaat atatgaaaaa
      301 agaattgttt ggacgttgcc cgtttgatct cgacgaagat gtaaaaaaaa tagccgcgat
      361 aaataaatat atccatgcaa aatttgagga gcataaaaat aaaatagaca acctaaaggg
      421 tgtggaacaa aaattgatgg tcttacagta ctttaatgta ctgaatgaac ttagtaatca
      481 ggctatctct tctttgaata taggagcctt ttcagcatcg gaagtattag ctagggttat
      541 aatagaacag gctgccaatc aattttatat agcaatagat gatggcgaaa atgcacaagc
      601 attactcaag gctagtaaga aattagttca tagtaatgga aaacggtggc tggagtgttt
      661 aaaatccaaa ggaatgacta atccagcagc aaatgaaagg attcgcatgg gaaaagaact
      721 aactgatcta tttaatagat tgtggccaaa tacacctgaa tatccaggaa ccagaaaact
      781 ttttgaatta attggctggg aaacacacta tcatgcccat tacgttcccc tttgtgattc
      841 aatacataca ttctctgatg atatggtaaa tatagtatct ctatataatg caatccaaag
      901 tgatgaaact actgctatcg aattcattct tgcagttaaa caagaaaata aacggctggc
      961 aatttataat tttgtaattg ctacggccct tcggtgtgag gccttagtaa atgtatttaa
     1021 ctctttaggt tatcaggata ttataacaga aatgacacca acaatagata cagtcaacca
     1081 gactataatt cgatatgatg attttgatca ttccagaatt gatgattgtc aataattaat
     1141 tgac
//
LOCUS       DADLSE010000351         1139 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLSE010000351 DADLSE010000000
VERSION     DADLSE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.355"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..265)
                     /locus_tag="JN692_004380"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="JN692_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /locus_tag="JN692_004381"
     CDS             complement(380..1018)
                     /locus_tag="JN692_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266453.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          332 a          280 c          174 g          353 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactctt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tgttttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gagtctaaa
//
LOCUS       DADLSE010000352         1127 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLSE010000352 DADLSE010000000
VERSION     DADLSE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.356"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            142..>1127
                     /locus_tag="JN692_004382"
     CDS             142..>1127
                     /locus_tag="JN692_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266454.1"
                     /translation="MYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAILAGVATTAV
                     WLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDNLQVYSKEQ
                     SDQRYVHREGDTLSGGLTFENDSTLAWIRNTDWAKIGFKNDADSDTDSYMWFETGDNG
                     NEYFKWRHRLAGGRVKDLMNLKWDTLNILVNAVINGCLGIGTTNALGGSSIVLGDNDT
                     GFKQNGDGLLDVYANGQRVFRFQNGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNGGV
                     QDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRP"
BASE COUNT          309 a          240 c          308 g          270 t
ORIGIN      
        1 aaaaacagta ttccggacaa ggcgacgcag cagaccagag agagcggtaa tgctgcgtat
       61 gattcaggtt ttcctccggt gaccatgaca gcggtctcag cgggaggtat accgccacac
      121 ggcaaggatt ttaacggtct gatgtacgat attaccgcag caatacggtt cgcccaggct
      181 ggcggtttgt acacgtataa tgccggtttt gcgggggcca ttggtggata tgcaaaagga
      241 gccattctcg ccggagtcgc aacaacagcg gtctggctga ataccacaga cgataacctg
      301 accgatcctg aaggctccga cagtgcgggc tgggtaaatc ttcttgagga tccgaaaagg
      361 atattcctgc ggcagaagaa caatctgtca gaccttcaga ataaagggac ggcacgggat
      421 aatcttcagg tttacagtaa agagcagtca gatcaacgct atgttcatcg ggaaggcgat
      481 accttgtccg gtggacttac ttttgaaaac gactcaaccc ttgcttggat tcgaaatact
      541 gactgggcaa agattggttt taaaaatgat gccgacagcg atactgattc atacatgtgg
      601 tttgaaacag gtgacaacgg caatgagtat ttcaaatgga gacaccgcct cgctggcggc
      661 cgggttaaag acctgatgaa tctcaaatgg gatacactaa atattctggt taatgccgtc
      721 attaatggtt gtcttggaat tggtacgacg aatgcgttag gtggaagttc aatcgtttta
      781 ggagataacg ataccgggtt taaacaaaat ggcgatggtc tgcttgatgt ttatgcgaat
      841 ggacagcgtg tatttcgttt ccagaatggc agtatccaga gtaataaagc tgtaaacgtt
      901 acaggacggg taacaccgtc agactacgga aactttgatg cccgttacca gcagcgaaat
      961 ggtggcgtgc aggatgtgcg gtatggttat gaaatgtatt acaaccctgg cagcaacacc
     1021 gtttcatgga cgtttcgctc accttcggga cacgggctgt cagggatatc gatatcggat
     1081 accggccgta actcagcgga taacgtcaac ggtgtgtatt accgacc
//
LOCUS       DADLSE010000353         1115 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLSE010000353 DADLSE010000000
VERSION     DADLSE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.357"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            83..1114
                     /locus_tag="JN692_004383"
     CDS             83..1114
                     /locus_tag="JN692_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HAY7266455.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     GNPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          304 a          287 c          298 g          226 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgctcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agaacatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctatgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gggaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 ccgctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt
     1081 ccccggaggc aaacatggac tggcaaaagt gtagc
//
LOCUS       DADLSE010000354         1114 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLSE010000354 DADLSE010000000
VERSION     DADLSE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.358"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..261
                     /locus_tag="JN692_004384"
     CDS             <1..261
                     /locus_tag="JN692_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7266456.1"
                     /translation="DGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQ
                     FKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            263..>1114
                     /locus_tag="JN692_004385"
     CDS             263..>1114
                     /locus_tag="JN692_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266457.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSNEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSH"
BASE COUNT          347 a          200 c          225 g          342 t
ORIGIN      
        1 gatggttcga tccgctttaa caataagatg ccttatcacg ttacgctggg ttatatcgga
       61 acaaacggaa tcacaatgtt gccacaaacg caaagtttaa tggtaacccc ttatagccat
      121 gcagatacgc agtttaaaaa tgttccgtca gcttttcagg ttggttatat caatgatttt
      181 ggtggattaa gtttttatga aataaactgc ccgacggtta ataactcttg taatgtttcc
      241 gtagccaaac gagataaata atatgcgcta cttattaatt gtaattatat ttattatagg
      301 ttttagtgca ttacctgtat gggcaatgga ctgctatgct gaacatgaag agggaaatac
      361 cgtagtcatt ggttacgtac caagaatttc tatccccagt aatggtaaaa aaggtgataa
      421 gatctggcaa agcaatgagt attttatgaa tgtcttctgt aataatgcat tacctgcccc
      481 atcacctgga gaagaatacc catcagcctg gactaatata atgatgtttt taccaggcgg
      541 tcaggacttt tataatcaga actcctatat ttttggcgta acatacaatg gagttgatta
      601 tgattcgact gcccctaatg ctatcgcagc accagaatgc attgatataa aaggagcggg
      661 tacctttaat aatcactata aaaaccccgc tgtctgtagt ggaggtcctg agcctcaatt
      721 atcagtaacc tttccagctc gcgtacaact ctatattaaa ttagcaaaaa atgccaatag
      781 agttaataaa aacctggtgt tgcctgatga atatatcgct ctagagttta aaggtatgag
      841 tggcgcagga gctatagaag tagacaaaaa tttgaccttc aggattcgtg gattaaataa
      901 tattcatgtc cttgactgct ttgttaatgt cgcactggaa ccagctgatg gcgttgttga
      961 ctttggtaaa ataaattccc gaacaattaa aaataccacc gttagtgaga cgtttagtgt
     1021 agtcatgaca aaagatcctg gtgcggcctg tactgagcaa ttcaatattt tagggagctt
     1081 tttcactacg gatattttga gtgattatag ccat
//
LOCUS       DADLSE010000355         1086 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLSE010000355 DADLSE010000000
VERSION     DADLSE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.359"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>1086
                     /locus_tag="JN692_004386"
     CDS             <1..>1086
                     /locus_tag="JN692_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266458.1"
                     /translation="LSDGKNENGQLFDKQKALKRIKELEGEQIKTARREMVLAVVGTM
                     KAGKSTTINAIVGQEILPNRNRPMTSVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQ
                     EKLATPAGQQVAQTLQQTGDTRELLDILTDDGWLKNEYHGEEEIFTGLASLNDLVRLA
                     AAMGSEFPFDEYAEVQKLPVIDVEFSHLVGMDECQGTLTLVDTPGPNEAGQPQMEVMM
                     RDQLQKASAVLAVMDYTQMNSKADEEVRKELNAIADVSVGRLFVLVNKFDEKDRNGDG
                     ADAVCQKVPAMLNSDVLPASRVYPGSSRQAYLANRALHELRKNGTLPVDEAWVDDFVR
                     EAFGRMKKDYVCKDSELATEGATDLWE"
BASE COUNT          294 a          264 c          307 g          221 t
ORIGIN      
        1 tgttatctga cggtaagaac gaaaacgggc agctttttga taaacagaaa gcactgaaaa
       61 gaattaagga gctggagggc gaacaaatca aaaccgcccg cagggagatg gtgctggctg
      121 ttgtcggcac gatgaaagca ggcaaatcaa ccaccatcaa cgccattgtg gggcaggaaa
      181 ttctgcctaa ccgtaaccgc cccatgacct ctgtaccgac gctcatccgc cacgttcccg
      241 gaaaaactga gccggttctc catctggaac atattcagcc tgtccgcaat ttattaatca
      301 cactgcagga aaaactcgcc accccggcag gacagcaggt cgcacagacc ctgcagcaaa
      361 ccggggatac ccgcgaactg ctggatattc tgacggatga tggctggctc aaaaatgaat
      421 accacgggga ggaggaaatc tttaccggac tggcatcgtt aaacgatctg gttcgtcttg
      481 ctgcggcaat ggggagtgaa tttccttttg atgaatacgc agaagtgcag aaactgccgg
      541 tgatcgacgt ggaattcagc catctggtgg ggatggatga atgccaggga acactcacac
      601 tggtggatac ccccggccct aatgaagccg gacaaccgca gatggaagtg atgatgcggg
      661 atcaactgca gaaagcctct gcggtgctgg cggtgatgga ctacacccag atgaactcga
      721 aagccgatga ggaggttcgt aaagagctta atgccattgc tgatgtgtca gtcggccgcc
      781 tgtttgtgct ggtcaataaa tttgatgaaa aagaccgtaa tggcgatggg gctgatgcag
      841 tctgtcagaa ggtaccggca atgctgaaca gcgatgtgct gcccgcctcc cgcgtttatc
      901 ccggatcctc acgccaggca tacctggcta accgtgcgct tcatgagtta cggaaaaacg
      961 gaacccttcc tgttgatgaa gcctgggtcg atgattttgt cagggaagcc ttcggtcgca
     1021 tgaaaaaaga ttacgtctgt aaagacagcg aactggcaac tgaaggggcg acagacctgt
     1081 gggaag
//
LOCUS       DADLSE010000356         1079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLSE010000356 DADLSE010000000
VERSION     DADLSE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.360"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..391
                     /locus_tag="JN692_004387"
                     /pseudo
     CDS             1..391
                     /locus_tag="JN692_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343264.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
     gene            289..711
                     /locus_tag="JN692_004388"
     CDS             289..711
                     /locus_tag="JN692_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7266459.1"
                     /translation="MRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGL
                     CANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            689..985
                     /locus_tag="JN692_004389"
     CDS             689..985
                     /locus_tag="JN692_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7266460.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          348 a          239 c          274 g          218 t
ORIGIN      
        1 caggcaacgg ctgaaacagt ggaaccgaat gcaactgaac atcatcagaa cacgcagccg
       61 ctggatgctc agtcacaggt aaattctgtt gatgagaaat atcaggaact gcgggcagaa
      121 ctccatgaag cccggaaaaa cattccgctt aaaaatcctg tcgatgcaga caaattactg
      181 gctgcctctc gcggagaatt tgttgaaggg attagcgacc cgaatgattc gaaatggatt
      241 cccggtcatc atatctcctc aaatgaagtc aaaaaaacgg aaaatgaagt gcgacagact
      301 gaagataaac agcaccagaa cagcgaaccg gaggagggaa caccctgcaa tcagactggc
      361 gaggataact gccctgactg tggcgcggtg atgggagacg caacttatca ggaaactttt
      421 gacgaaaaaa aaccagatga agcacaggaa gaagagccga agaaaacgga aaaagctgat
      481 gatttgttac cggagaacgc tggcagcgat cagcataatg atagcaataa tgaaactggc
      541 gaagatgaac tcaactggaa aaaacaggtg ttgattgcgg cggtttacgg tttatgtgcg
      601 aatcctgcgt gcatagccta tgcgcctgca atccctgaca tcgcaatgat gattgccaac
      661 aagcttgaaa attttggagt gaccagtgat gagcgcttac ccgatttttg atcgtattga
      721 agaaatggca tgggctcgcc actaccagca gatcgttcgt gaagaaaaag aaacggaact
      781 ggcggacgac ctggaaaaag gtctgcctca gcacctgttt gaatcgctct gcatcgacca
      841 tttgcaacgc cacggtgcca ataaacaggc aatcagccac gcatttgatg atgatgttga
      901 gtttcaggag cgcatggcag aacacatccg gtacatggct gaaaccattg cccaccacca
      961 ggttgatatt gatttggagg actaaaatct atggcgcttt ttcaacgagc aataaacaca
     1021 caggcctatc taaaagcagg aattatgggg tttgctggcg acggcaaaac ctacacagc
//
LOCUS       DADLSE010000357         1040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLSE010000357 DADLSE010000000
VERSION     DADLSE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.361"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            92..274
                     /locus_tag="JN692_004390"
     CDS             92..274
                     /locus_tag="JN692_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HAY7266461.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     RVTELFALLHTEEGCC"
     gene            complement(375..704)
                     /locus_tag="JN692_004391"
     CDS             complement(375..704)
                     /locus_tag="JN692_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7266462.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
BASE COUNT          269 a          237 c          238 g          296 t
ORIGIN      
        1 ccgttcagac ggcaggtgcc tgcttttccg gcaacaagca ggaatctcag cgctgaagca
       61 ggaattagag gtaaaaacgc cctaccgggc catgaatcac ccggtcattg gagtagtaac
      121 caaagcagat ttagccagca tggaacaaat atctttggtg aaaagttggt tacgggaagc
      181 tggagcgcac aacgtgttag taaccagtgc agttaataac aatagagtta cagaactttt
      241 tgccttgctg catacagaag aaggctgttg ttaatttcga ttggttctca gatgagaatg
      301 atgacggtga tacacagata agaatcaatg agcaatgagg gttgccgggc aaccctcatt
      361 gaataaaacg ggaattactt cgcttcgccg tttttcattt cgcccatagc tttcagcttt
      421 ttggagatat cgcggcgttc tttggagagc tcggcatttt tgatgatgta atcgtcaacg
      481 cgatcttcat agtcaccttt catgctggca atgatgccct ggattgcttc aacgctcatc
      541 cctggcttga tgtaatcgct caggttgtcc agcaacagga cgcgcttctg gttgtcacgg
      601 atctttttct caacgtcctg aatttcacgt tgcagtttgt tcttacgacg gaacagacga
      661 acaaattcca gtacgtcctg gaatgaaggc ttggtagttt ccatttttat acccctgata
      721 atgtgagagt cggattcgtt taagcaaccg ctattcgtta gggccaacat tactcatagc
      781 tgccgcagat gacaatgctt ttatcccttt tcactatcat accctttatc cttgctgaat
      841 cgaagcagca gcaagatgat tctgaagttc aggaattatt tgcgcaaggc ccggcaaatc
      901 agattcgata gcaactcaag ctgatgtgcc gtttccatgt tcagccacac ataaccatag
      961 attggtgttt ccacgacttt caggtcatgg tggttatcga gcaactgacg cagctcttcc
     1021 acagcatcgt tcaatttttc
//
LOCUS       DADLSE010000358         1035 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLSE010000358 DADLSE010000000
VERSION     DADLSE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.362"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..584)
                     /locus_tag="JN692_004392"
     CDS             complement(<1..584)
                     /locus_tag="JN692_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HAY7266463.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPT"
BASE COUNT          308 a          235 c          208 g          284 t
ORIGIN      
        1 gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg
       61 gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacaa
      121 cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg
      181 cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga
      241 tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg
      301 attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt
      361 tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta
      421 tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt
      481 aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat
      541 cgcgttaagc caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct
      601 tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc
      661 tgactctgtc cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta
      721 acgccgataa actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct
      781 taacattgat cgttatcaat taaaattaca aagcaagaaa ttataagcga actgatattt
      841 attatcattt gaaataaatt taacttaagc gacaatcatt tgtgacgttc attgcagaaa
      901 ataaaaataa cattatcgct ataaattaaa atacataccg aaattcgtat aatttcagca
      961 gttgttattt agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt
     1021 accgcctcat cctga
//
LOCUS       DADLSE010000359         1023 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLSE010000359 DADLSE010000000
VERSION     DADLSE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.363"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            83..601
                     /locus_tag="JN692_004393"
                     /pseudo
     CDS             83..601
                     /locus_tag="JN692_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          299 a          235 c          218 g          271 t
ORIGIN      
        1 gttacgccga atctgctgaa tcagaacttt acgcccacag cacctaatca ggtctgggtc
       61 gcagattcag ttgtccaggc cttccgaaat caaccgactg aaggcgctgg tcgcgaaact
      121 gcagcgtatg cagttcggta aaagctcaga aaaacttcgc gcaaaaaccg aacggcggat
      181 acaggaagca caggagcgaa tcagcgcact tcaggaggaa atggcggaaa cactgggtga
      241 gcaatatgac ccggtactgc catcttccct gcgccagtct tcagcccgta aaccgttacc
      301 agcctcactt ccccgtgccc cccgggtcat ccggccggaa gaggaatgct gtccggcctg
      361 tggtggtgaa ctcagtccac tgggatgtga tgtgtcagag cagctggagc ttatcagcag
      421 cgcctttaag gttatcgaaa aacaacgccc taaactggcc tgtcgccggt gcgaccatat
      481 cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt tatgccggag cggggcttct
      541 ggcccatgtt gttaccggaa agtatgcaga ccatctgcca ttataccgcc aaagtgatct
      601 actattccac acagctattt agctctcttt ttgttgagct cccatgattc tttcatttat
      661 tgttactaat tattttatgt tatcatgatg ttagaacacg ctctgctctg tgtctctatt
      721 ttatctatca tattctgagg attaactaca taacaaggcg caagatatat atccaaaaac
      781 aaataatacc gttgcaataa ttcctttcag taaaggaggt gtgattaatt tcaggggatt
      841 tcagagtgaa aattgtacaa catattaaaa tatgttcatt tcgttataat acacacaaga
      901 aaatattaag ttagataata tattctaaat aaccatagat aaaaacgcac ataatgggga
      961 atattttatg aaaatacctg aagcagtaaa tcatattaat gttcagaata atattgatct
     1021 tgt
//
LOCUS       DADLSE010000360         1018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLSE010000360 DADLSE010000000
VERSION     DADLSE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.364"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>1018
                     /locus_tag="JN692_004394"
     CDS             <1..>1018
                     /locus_tag="JN692_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY7266464.1"
                     /translation="KPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINT
                     AFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQS
                     EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYN
                     VLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEER
                     LAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEID
                     NNIGE"
BASE COUNT          273 a          248 c          297 g          200 t
ORIGIN      
        1 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
       61 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
      121 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
      181 gtgtgatgtc atcgttcagg caccacttcc ccctaaaccg atcgaacgcg gttatgccag
      241 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
      301 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
      361 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
      421 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
      481 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
      541 cctgccggca gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct
      601 ccacctggca aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact
      661 gtacgaaacg ggccgggtga aggaagccgg gtgcctggcc cacgcccgcc gaaaaatcca
      721 tgacgaggat gtgcgccgtc cgacagaaat gactcaggaa gcgctcagac ggatagcaga
      781 gttatacgac atagaagcgg agatacgtgg cagtccggca gaggaacggc ttgcagtcag
      841 aaaagccaga agcgtccagt tgatgcagtc gttgtacgac tggatacagt tgcagaggaa
      901 aacgctgtcg aaacatgcgg agatggcgaa ggcgttcgac tatatcctga atcactggaa
      961 tgcgctgaac gagttctgtc gtgacggctg ggtggaaata gacaacaaca tcggtgaa
//
LOCUS       DADLSE010000361         1011 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLSE010000361 DADLSE010000000
VERSION     DADLSE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.365"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN692_004395"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN692_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7266465.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          292 a          163 c          244 g          312 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc a
//
LOCUS       DADLSE010000362         1006 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLSE010000362 DADLSE010000000
VERSION     DADLSE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.366"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..992)
                     /locus_tag="JN692_004396"
                     /pseudo
     CDS             complement(1..992)
                     /locus_tag="JN692_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969972.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          299 a          262 c          217 g          228 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gatgaatacc
      121 agcgcagcta cttgtcccag ggtattcgca atggcgtaaa tcaccattaa agtattaccc
      181 atgttgtaat actcgcccag cttctgaaag gcgtaatatt gaccgttcgt cattaagtca
      241 tccgggatat tacgcgagtc aaacatcatc cccatcgcca gtgagcccag aatggcacaa
      301 accgcaacca tcaccgccag gcatagcatc ccttttggaa attcttttcc tgggttgcgc
      361 gtttgattaa cgtaaggaga aatcttctct gctccgccaa ccgcgaaaac cagcattgaa
      421 atagtggtaa tgtaggtaaa gtcgatatga ggaatgaacg tttcccaggt aatgtttgtg
      481 gtcgcaatat gcacttcagt aatcgcaggc gcggttaccg ccatcgccac atacaggagc
      541 gacataacaa acatcgcaat ccctgccaca gaaccgacga ttttcagcga tttcataccg
      601 cgtgaagcaa cccacataaa gaagataaac agcaccagcg ttaacccctg taacgctacg
      661 actgaatatt ctttgattag cgaaccgtcg cctttcatcg cccaaccgag cgcaatcaga
      721 attgcctggg gtttttgtgc cagataggga atatgcacca cccagtaggt ccacgcggcg
      781 agataagcca gtccgggtcc catcgtgtgt ttaatccagg tactgacccc gcccttccca
      841 tctttgaagg tcgagcctaa ctgaccaaca attagcgcat aaggtgtgaa atagagtgca
      901 aagataaaca cccatgaaaa aacaaccacc agcccctggt tggcatagtt attaacaacg
      961 ttgccaaaac cccagacagt gataaaagac atcagcgcaa tgttgt
//
LOCUS       DADLSE010000363          991 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLSE010000363 DADLSE010000000
VERSION     DADLSE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.367"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            81..991
                     /locus_tag="JN692_004397"
                     /pseudo
     CDS             81..991
                     /locus_tag="JN692_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT          300 a          220 c          232 g          239 t
ORIGIN      
        1 gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt tagttactgg
       61 cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat ggcattaacc
      121 gatatcaaag tgaaaaccgc aaaacccaaa gacaaaccct ataagcttgc tgatggcggc
      181 ggtatgtatc tactgattaa cactaatggt tctaaatact ggcgcatgaa gtaccgcttt
      241 gctggtaaag aaaagatgct ttctattggc gtataccccg atgtaacgct agccgatgct
      301 cgcgagaaac gcagtgaagc caggaaactc ctcgctgctg gaggcgatcc aggggaggca
      361 aaaaaggaag aaaaaatcgc gcagcaaatg agtctgaaaa atacgttcga ggctattgct
      421 cgcgaatggc accagttaaa agctgatcgg tggtcattac gctatcgtga tgaaatcatt
      481 gatacctttg aaaaagacat ttttccctac attggcaagc gccctattgc cgaaatcaaa
      541 ccaatggagt tactggaagc actgcgtaaa atggaaaaac gtggagctct ggagaaaatg
      601 cgcaaggtcc gccagcgctg tggtgaggtg ttccgctatg caattgttac tggccgggca
      661 gactataacc cagcccccga tcttgccagt gctttagcta caccgaaaaa agtacatttt
      721 ccctttctta ctgctaatga acttccccac ttcctcaacg atctggcggg ttataccgga
      781 agtataatca ctaagacagc tactcagatc attatgttaa ccggcgtacg aacacaagaa
      841 ctgcgttttg cacgctggga agatatcgat tttgagacaa aattgtggga gatcccggca
      901 gaagtaatga agatgaagcg acctcatatt gtgccgctct ctgagcaggt cattatgcta
      961 tttaagcagc ttgaaccaat ctcaaaacat c
//
LOCUS       DADLSE010000364          986 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLSE010000364 DADLSE010000000
VERSION     DADLSE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.368"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            188..>986
                     /locus_tag="JN692_004398"
     CDS             188..>986
                     /locus_tag="JN692_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266466.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLG"
BASE COUNT          284 a          204 c          228 g          270 t
ORIGIN      
        1 acaaaaatga aggtactgcc gaaaatattc agatagagct gagggatgac caagatgctg
       61 cgttaaaaaa tggcgatagc aaaacggtta ttcgaggcgc taatcaatgt gatctacacc
      121 tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt taaccttagg
      181 atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc ttctattaat
      241 ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta ttggagcagg
      301 tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga atctggttgt
      361 agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg acactgatca
      421 tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata aaggatcgct
      481 gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg tattggatat
      541 tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag ttggggcggc
      601 cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt ataaaattgc
      661 aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa ataacagtgt
      721 tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcaccgtgg atctcccgga
      781 ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac aaaagctctc
      841 attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta acacagcacc
      901 tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa aaatactggc
      961 tacgggagaa aatgtctccc tgggga
//
LOCUS       DADLSE010000365          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLSE010000365 DADLSE010000000
VERSION     DADLSE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.369"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN692_004399"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN692_004399"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7266467.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          260 a          220 c          221 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcggaatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgaaaaa aaacctcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADLSE010000366          981 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLSE010000366 DADLSE010000000
VERSION     DADLSE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.370"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            194..981
                     /locus_tag="JN692_004400"
                     /pseudo
     CDS             194..981
                     /locus_tag="JN692_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          325 a          209 c          170 g          277 t
ORIGIN      
        1 ggttgaacta tatccctgct tttcaaccat cctaaaaaag catgaattgc tcgctctacc
       61 ctaagtggct gtgctatttt agaaaaactt aatcctgagt taagcaaaaa aacataatca
      121 ataaaaatac atacgcaaaa aacattgatt aagtgaatat accatggaag aaaaatataa
      181 accggagtag tgtatggcca atctctacga cttgaaaaag ttcgacttaa atcttcttgt
      241 catatttgag tgtatttacc agcatttaag cattagcaaa gctgctgaat cactatatat
      301 caccccatca gccgtgagcc agtcgttaca acgcttacgt gcgcaattta atgacccttt
      361 attcattcgc tcaggaaaag gtatcgcgcc gacgacaaca gggttgaatc tgcatcacca
      421 tcttgagaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
      481 aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg
      541 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
      601 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
      661 ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt
      721 tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa
      781 gagttcaccc aattaatatc caaatcagca ggagttgatg attttcaaat ggaaatagac
      841 gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat
      901 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ctcccaccgt
      961 gactttctta atttaaaaga g
//
LOCUS       DADLSE010000367          975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLSE010000367 DADLSE010000000
VERSION     DADLSE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.371"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN692_004401"
     CDS             complement(36..551)
                     /gene="sfmF"
                     /locus_tag="JN692_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HAY7266468.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(562..>975)
                     /locus_tag="JN692_004402"
     CDS             complement(562..>975)
                     /locus_tag="JN692_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7266469.1"
                     /translation="CELNAGQIVEFDFGDIGASLFSAAGPGNRPAGVMPQTKSIAVKC
                     TNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITPNDLNSVIPFRLDAA
                     AAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
BASE COUNT          258 a          266 c          219 g          232 t
ORIGIN      
        1 atcgactacg ccaaattata cgaattaata taatattaat tataattgat cataaatatc
       61 gcatccgctt tcgccacacc tggccgaaca ccgctggcta acgctcgata attggcaaaa
      121 aagtttagtg tgacattgcc gtttgcatct acttcctcag tcgggctcgc ctcccccagt
      181 gcgagccggg agcgatcgct attacgtaat tcgatggcga cggtttgcgc cattgcggga
      241 tcatccagag ccagcaggtt ggtatcggat gccggcgttc ccgtaaataa aatcgcaact
      301 gaacccggag gacatccctc cagccgcagg ctaaaaggga cgagtgccgt ggtatcgcca
      361 gcgttcagta attgtgtcgt aggccatctg cctaaatcta ccgtcttatc aatatccgct
      421 gtgtttacgg tacaggagaa atcaacaacg ttaccgtgca aattgatatt aatcgttcct
      481 aaagggtcag ctgcccatcc actggaactc cacagtaacc cgcagaaaca gctaaagaat
      541 actcttctca ttctccgtac ctcattgata atcgacgcgt aaatacccca gcgcgctaaa
      601 cggcccttcg gtcggttttt gaccggtaat actgataggc caggcgcgaa gtgtgacatt
      661 ggctgccgca gctgcatcca gacggaaagg aataacgcta ttgagatcgt taggcgtgat
      721 cggcgtatcg ttctgatcgg cgacaataaa acctaaatcc tgattgtccg acaccatcgc
      781 ctgaccagaa acggcactgg cttccagacg cattgttaaa taagcctgcg cagcaacatt
      841 cgtacatttg accgcaatgc tcttggtttg cggcatgacg ccagctggtc gattccccgg
      901 ccctgccgca ctaaataacg atgcgccgat atcaccaaaa tcaaattcaa caatctgccc
      961 ggcatttaat tcgca
//
LOCUS       DADLSE010000368          965 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLSE010000368 DADLSE010000000
VERSION     DADLSE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.372"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..483)
                     /locus_tag="JN692_004403"
     CDS             complement(1..483)
                     /locus_tag="JN692_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7266470.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="JN692_004404"
     CDS             complement(474..776)
                     /locus_tag="JN692_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7266471.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          293 a          236 c          195 g          241 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctgggta caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgc
//
LOCUS       DADLSE010000369          956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLSE010000369 DADLSE010000000
VERSION     DADLSE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.373"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(304..747)
                     /locus_tag="JN692_004405"
     CDS             complement(304..747)
                     /locus_tag="JN692_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266472.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVAAVVRTALRAVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          253 a          231 c          253 g          219 t
ORIGIN      
        1 ccggctcatc aaccatttgc cggagcagat tgatgctcag ttgcaaaagc cggtcggctt
       61 catcacaaag cagggtgatg ttcttttcgt gcattattta accttcctga gacgcatttt
      121 catgacaacc acggaggagg ccatttacta caaacgatcg tttatagcga tcaatataat
      181 agcaacgatc gttatattct attaatatga aaaacacaac gcaaggggga caacccgggt
      241 atggctgcgt ttgcattgtg ttttcataga gttaaaaatc tgtaacgaca acccggcaca
      301 tcactaccgg gttaagaaga aaatccagtt ccataccgcg cgggccacag agaccacggt
      361 gtcacgaacg gcgcgcaggg ccgtacggac aacagcggca acacagacac tctccgccgt
      421 gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg gtctgagtgc ggacagactc
      481 cgtgcgcagt tcgtcctgca ggcgtgtcat cagagggatg gcggcatgtc cgggaagcac
      541 tgtcatgagt gcactgaccg gggtgacctc cccctgaaac agtgcattac agagtgagga
      601 tttaccggcc ccgcttttac ccataatgcc aggcacgggt tcgtgacggg ggagtttgcg
      661 cagatgttcc aggatgtgac gggaaatcgg gtaaggcagg gaggagagcg gtttttcaat
      721 tgcctcaatg gcatcagacg gattcataca tgtattccca gtgaaaaaag acaaaacccc
      781 gtgcaccgga aaggtggcgg gggattcacg gagataatat ctttctgaaa ttattttgaa
      841 tgcaggtata tgtggctgat ttgtatcagc taatgagtac aggtctgaat ctaatctgac
      901 agtccgcttt gtgccaggag cggactgacc tgctccccga tgattaatac acagct
//
LOCUS       DADLSE010000370          926 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLSE010000370 DADLSE010000000
VERSION     DADLSE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.374"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..97)
                     /locus_tag="JN692_004406"
     CDS             complement(<1..97)
                     /locus_tag="JN692_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266473.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNE"
     gene            236..907
                     /gene="queE"
                     /locus_tag="JN692_004407"
     CDS             236..907
                     /gene="queE"
                     /locus_tag="JN692_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7266474.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          260 a          193 c          219 g          254 t
ORIGIN      
        1 attcgttatc attaaaaact aatacagaaa aaataaatat aattttggtg aatgttttga
       61 catgattaaa tccattttct ttattttctt ctgacataat acgttactcc ctttaattat
      121 taacaatatg tttcgtattt ttctttaaat ctgcatgtaa cagaatgtta ataagacatg
      181 attgttgcgg caggatctgc aataattaac aattatccac ttcacagaga atgctatgca
      241 gtacccgatt aacgagatgt tccagaccct gcaaggtgag ggttacttta ccggcgtccc
      301 cgccattttt attcgtttac agggatgccc ggttggctgt gcctggtgcg acaccaaaca
      361 cacctgggaa aagcttgagg atcgggaagt ctcccttttc agcattctgg cgaagaccaa
      421 agagagtgat aagtgggggg cggcgagcag tgaagacttg ctggctgtca ttggtcgtca
      481 gggatatacc gcgcggcatg tggtgattac gggtggtgag ccttgcattc atgatttgct
      541 gccactgact gacctgctcg aaaagaacgg ttttagctgc cagatcgaaa ccagtggaac
      601 tcatgaagta cgctgcacac cgaatacctg ggttaccgta tcgccaaagc tgaacatgcg
      661 cggcggctat gaagtgttgt cacaggcact ggagcgagcc aacgaaatca agcatccagt
      721 ggggcgcgta cgcgatattg aagcactgga tgaactactg gcaacgttga ccgatgataa
      781 accgcgagtc attgcactgc agccgattag ccaaaaggat gatgccacac gattgtgcat
      841 tgaaacctgt attgcgcgta attggcgttt gtcgatgcaa acacataaat atttaaatat
      901 tgcctgatta cacatttata agcgtt
//
LOCUS       DADLSE010000371          910 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLSE010000371 DADLSE010000000
VERSION     DADLSE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.375"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            157..821
                     /locus_tag="JN692_004408"
                     /pseudo
     CDS             157..821
                     /locus_tag="JN692_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT          287 a          192 c          199 g          232 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacaccagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgcgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcgtaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaatat tgctatttta taccagcaat acaatcgtta aattaaatgc ccggcaaata
      841 ccgggcatca agtaattact caaatcgcca cgcaatattc atgccgacac cataatttcg
      901 tccgggcgca
//
LOCUS       DADLSE010000372          906 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLSE010000372 DADLSE010000000
VERSION     DADLSE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.376"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            62..>906
                     /locus_tag="JN692_004409"
     CDS             62..>906
                     /locus_tag="JN692_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7266475.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATVALINGASLTLTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIK"
BASE COUNT          269 a          190 c          207 g          240 t
ORIGIN      
        1 agacagacat agcattatgt gatctactga tttgtgccaa caattcaatg agatcactgc
       61 tatgcctgtt cataaagtat gtcagaattt cttccgggat gccttagcac catttcatca
      121 gtaccgacaa aatgccctga tggatgccac agtggcgtta atcaacggag catctctgac
      181 gctgaccagt atcggacgtt ttttgccggg taatgcacaa gttaagaata agatcaaacg
      241 tattgatcgc ctcatgggta atgaggcgct ccatcgcgat attccaatga ttttcaggaa
      301 tatcacttca atgctgacac ggcagttatc tttatgtgta atagccgtag actggagtgg
      361 ctatccctcc caggagcatc atgttcttcg tgccagcctt ctgtgtgacg ggcgttccat
      421 tccgttattg agtaaggttg ttccttctga aaaacaaaat aatccattga tacaacatga
      481 tttccttgat tcccttgctc aatccctgcc ccccgatgcc agggttatta ttgtcacgga
      541 tgctggattt cagagcgcat ggtttcacca tattacttct ctgggctggg attttattgg
      601 tcggatcaga aataacgttc agtattgcct cgataacgcg cctgaaagat ggttaaaagt
      661 gtcggatagc cctgaatgta aaacaccgga atatatgggg gctggaaggc tggttaaaga
      721 aaggaaaaaa agcatccggg ggcattttta cacctataag aaatcggcaa aagggcgaaa
      781 aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca gacaaagaac agagcaaatc
      841 agcaaaagaa gcctggttga tcttcagcag tacaaatgac ttcagagcgc gagaaatcat
      901 caagtt
//
LOCUS       DADLSE010000373          896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLSE010000373 DADLSE010000000
VERSION     DADLSE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.377"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            28..>896
                     /locus_tag="JN692_004410"
     CDS             28..>896
                     /locus_tag="JN692_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266476.1"
                     /translation="MSENKSFSTAVPAVQISDSGLNVPDEADILSGRLADFSAALGGA
                     MSTSLSSPQGQLASSESAIIADKNDQLLYIVNQINPDFSSGRFQDAIGRIYFLERRGA
                     TGTTVTATCTGLVGTLIPAGSMVQDEAGYKYVSQSDVTIGASGLVDAVFLNLSTGPVG
                     CPVGTLNKIYKAIPGWSGVTNASAGVPGSDEETRADFENRRRNSVARNARNILEAIRG
                     EILSTVENVVDVYVTHNPKKTEQKAGVSQYPLTPGSLYVGVYGGSPADIAAAIWRKAP
                     PGIDMSGNTTFTVA"
BASE COUNT          211 a          215 c          260 g          210 t
ORIGIN      
        1 caataatgac atttttacgg tactgctatg agtgaaaata aatctttttc taccgcagtg
       61 cccgctgtac agatttcgga tagcgggctg aatgtgccgg atgaagcgga tattctgagc
      121 ggcaggcttg ctgatttttc ggcggcgctg ggcggggcca tgagtaccag tctgagcagt
      181 ccgcagggac aacttgcatc cagcgaaagc gccattattg cggataaaaa cgatcagttg
      241 ctgtatatcg ttaaccagat caaccctgac ttttccagtg gtcgctttca ggatgcaata
      301 ggcaggattt atttcctgga gcgccgcggg gccacaggta cgacagtaac agcgacctgt
      361 accgggctgg ttggcacgct gatcccggcg ggcagtatgg tgcaggatga ggccggttat
      421 aagtatgtca gtcagtcaga cgtcactatc ggcgcatcag ggctggttga tgcggtattt
      481 ctgaatttgt ccaccggccc cgtcggctgt cctgtgggga cgctgaataa aatttataag
      541 gcaatacctg gctggtcagg tgtcactaac gccagtgccg gtgtgccggg cagcgatgag
      601 gaaacccgtg cggactttga aaaccgccga cgtaattcag ttgcccgtaa tgcccggaat
      661 attctggaag ccatccgggg tgaaatactc tccacggtag aaaacgtggt ggatgtttac
      721 gtcacccata atccgaaaaa aacagaacaa aaagccggag tcagtcagta tccattaaca
      781 cccggttcgc tttatgttgg cgtgtacggc ggcagcccgg cagatatcgc ggcggccatt
      841 tggcgtaagg ctccgccggg tattgatatg agtggcaaca caacgttcac tgttgc
//
LOCUS       DADLSE010000374          874 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLSE010000374 DADLSE010000000
VERSION     DADLSE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.378"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..226)
                     /locus_tag="JN692_004411"
     CDS             complement(<1..226)
                     /locus_tag="JN692_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266477.1"
                     /translation="MKWLKLIFWGLCGLQICQLSYAQEALTEKYSQSELLRNWALSHC
                     LSLVYKNDAVQDDARATASAYLEYGQQPVEI"
     gene            complement(223..709)
                     /locus_tag="JN692_004412"
                     /pseudo
     CDS             complement(223..709)
                     /locus_tag="JN692_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(762..>874)
                     /locus_tag="JN692_004413"
     CDS             complement(762..>874)
                     /locus_tag="JN692_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266478.1"
                     /translation="GYYDAKFFLRPHFQLEGLTGSLRLVTKLPSVKQGNA"
BASE COUNT          281 a          208 c          144 g          241 t
ORIGIN      
        1 aaatttctac aggttgctga ccatattcaa gataagcact agctgtagct ctggcatcat
       61 cctgaacggc atcattttta tacaccaatg acaaacaatg actcaaagcc caattgcgta
      121 agagctctga ctgagaatat ttttcagtca atgcttcctg agcataagat aattgacata
      181 tctgtagccc acagaggccc caaaatatta atttcagcca tttcatggta gtacccacag
      241 gattccgact tcaggaacaa atgttccatt atctggatca tttagtagat gacattgatc
      301 tgaacaaacg ctaccatccc ataatgttac atgtccccga gcattgctcc agccatggcc
      361 ttttacaaca atgatgcctt ttttacctgt aaagtcacca ggagttgtga ttattaatga
      421 caagatctgg ctcccccata atctgggtca gatgttcaat catatcattc acacgataaa
      481 tataaaattt cttatcagaa ccactaaccg ttgcataggg agaactacgg gatatcggaa
      541 atcccgtagc attcaaaaca taactcatac gaattggaca agcattttca aaatagccac
      601 ctgaaatatt ttgcccaaca tttccgccaa ttatacgacc gacatcagca acgggaaaat
      661 tcacttttcc aaatgccgac catgcctcat taaatgaagg tctacccatt ttctttaccc
      721 tctaaaaaat ctgcatcaca agctttatga aatataataa atcaggcatt accctgtttt
      781 accgacggca gttttgtgac cagacgcagt gatcctgtta acccttcaag ctgaaaatgc
      841 gggcgcagga agaatttagc atcgtaataa cccg
//
LOCUS       DADLSE010000375          873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLSE010000375 DADLSE010000000
VERSION     DADLSE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.379"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            92..449
                     /locus_tag="JN692_004414"
                     /pseudo
     CDS             92..449
                     /locus_tag="JN692_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            644..>873
                     /locus_tag="JN692_004415"
     CDS             644..>873
                     /locus_tag="JN692_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7266479.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVL"
BASE COUNT          205 a          237 c          250 g          181 t
ORIGIN      
        1 cttgccggtg ttctccgggg taaaaaggtc cacactaccg tcagccggaa agccgttgcc
       61 gcaggcgacc gcgtaaaccg tcatcaaggc aatgtgccgc gtactccggg tggcccgcag
      121 cggctggtat acgtggtgtc agcggcggac aaggataaac acacgtcagc agttccgtca
      181 gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg tgccccacgc
      241 ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg gcagccagtc
      301 tgcgccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc agttaccgtg
      361 cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc agtggcccga
      421 accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg gggaaacacg
      481 gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca tcaccggctg
      541 ctgatgaaag ccgtctgccg caggatcagt gatgcacgtt ttatgagact gctgtggaaa
      601 acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac agggcggtgt
      661 gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc tgaataaacg
      721 ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta tccagcgagg
      781 ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt actgttgcta
      841 tgccgatgac tgtgtgccac gaaggcgcgt cct
//
LOCUS       DADLSE010000376          846 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLSE010000376 DADLSE010000000
VERSION     DADLSE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.380"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>846
                     /locus_tag="JN692_004416"
     CDS             <1..>846
                     /locus_tag="JN692_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266480.1"
                     /translation="AGACRFVFNRALALQNENHEAGNKYIPYGKMASWLVEWKNATET
                     QWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYPQGVKLDQENSR
                     IFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPVHPSASMIGLDA
                     GVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCI
                     ANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWY"
BASE COUNT          240 a          208 c          217 g          181 t
ORIGIN      
        1 tcgcaggcgc atgtcgtttc gttttcaatc gtgctctggc gcttcagaat gagaatcatg
       61 aggccggtaa taaatacatc ccttacggga aaatggcttc ttggctggtt gagtggaaaa
      121 acgccactga aacgcaatgg cttaaagatt cgccctcaca gccattgcaa cagtcactga
      181 aagaccttga gcgggcttac aaaaacttct tccagaagcg ggctgctttt ccccgattca
      241 aaaagcgggg acagaatgct gcattccgct acccgcaggg cgttaagctc gatcaggaaa
      301 acagccgtat ttttctgccg aaacttggct ggatgcgcta ccggaatagc cgtcaggtca
      361 cgggagttgt gaaaaatgtc actgtcagcc agtcctgcgg tacgtggtac atcagtattc
      421 agacagaaag tgaagtatca actccggttc acccttcagc atcaatgatc gggctggatg
      481 ctggcgtggc taaactcgcc acgctgtcag atggcacagt ctttgagcct gtaaacagtt
      541 ttcagaaaaa ccagaagacg ctggcgagac ttcagcgaca gttaagccgg aaggtcaaat
      601 tcagcaacaa ctggcagaag cagaaacgca aaatacagcg actgcattcc tgtatcgcaa
      661 atatccgcag ggactacctt cacaaagtca caacgaccgt cagcaaaaac cacgcaatga
      721 tagtcattga ggatttgaag gtcagcaaca tgtcaaagtc agcagcgggt acggtcagcc
      781 agccggggcg caatgtccgg gcaaaatcag gtttaaaccg ttcgatactg gatcagggct
      841 ggtatg
//
LOCUS       DADLSE010000377          844 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLSE010000377 DADLSE010000000
VERSION     DADLSE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.381"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            147..>844
                     /locus_tag="JN692_004417"
     CDS             147..>844
                     /locus_tag="JN692_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7266481.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVGPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLMVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRT
                     AINNIKKFWSLTAKG"
BASE COUNT          215 a          186 c          254 g          189 t
ORIGIN      
        1 cgcggggggg tgggaagacg acagtcaggt gaaaagaatg ctggtggagt ggggaccggt
       61 tatcccggaa gggaaggtcg agatcactat cagtaagtac gagaaaacgg cgggtgcagc
      121 tgcctgatta agaggagaaa cgaagtatga ataatctgat ggtcattgat ggtattgaag
      181 ttcgtcgtga tgcttatggg cgttacagcc tgaacgatct gcatcgcgca gcagtagcat
      241 ctggtgcaaa tgccagaacc aaggagccag gaaagtttct ttccagccaa caaactgttg
      301 agcttgttca tgaattaacc aacacccaga atttgggtgt tggcccggtg agtgtgattc
      361 atgggggaaa tgaacgggga acgtatgtct gtaaggaact ggtgtatgcc tatgcaatgt
      421 ggatcagccc gtcattccat ctgatggtga tccgtacttt cgatatggta accagcgcgc
      481 cggaaaagtt atccggacag gctgctgaca agatgcaggc tggcgtgatt ctgctggact
      541 ttatgcgccg ggagttaaat ctgtctaact catctgtgct tggggcctgt cagaaactcc
      601 aggaggctgt tggcttaccg aatctggcac cgcgctatgc cattgatgca cctgctgacg
      661 cgcctgatgg ctcaagccgc ccgacgctgt cgctgagtgc actgctgaag cagtatggta
      721 tccgcctgac ggctaatcag gcatatcacc agatggcgaa gctggggatc gttgaacaac
      781 gcgaacgata cagccgtacc gcgattaaca acatcaaaaa attctggtcg ctgacagcga
      841 aagg
//
LOCUS       DADLSE010000378          839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLSE010000378 DADLSE010000000
VERSION     DADLSE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.382"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(91..456)
                     /locus_tag="JN692_004418"
                     /pseudo
     CDS             complement(91..456)
                     /locus_tag="JN692_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(729..>839)
                     /locus_tag="JN692_004419"
     CDS             complement(729..>839)
                     /locus_tag="JN692_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7266482.1"
                     /translation="FRTCCSRVDVWLNLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          220 a          200 c          216 g          203 t
ORIGIN      
        1 atcatcccgt aactgaattg tggaaacagt gacaccgcct ctctgataac agagaactcg
       61 cgtgtcgtaa cttttctggc atgaaactgg cgttgacgga tccttttgac tgaaatagca
      121 gtcttccagt ttttcgaaca catcccacgc ctgatcggtt tcgagcattt tggcgtggcg
      181 tgctgcgcct cgttctgtcc agaggatgag ggagcgggct ttgggtgaaa ctggattttg
      241 tgagtagttt aaagcgaccc gcaattcttt aaggtcatta ccaacaactt tgaaaaagtg
      301 tttcccttca acgaagcgta ctttgttctc atgatgattc tggcgaatac gcaccggctc
      361 agtgccgtaa agctgcgcca aaagttcggt ggtaataaca ggaatctggt tatgggtgat
      421 cggggagaga gtttcaacag agatttgagt ggtcataacg ataactccgt acatttggaa
      481 attatcgcca ccgacgacgc caatcgaact ggtggcgaac tgtgcagggt tggcgtaacc
      541 gggtacggaa accggcgagc ctttcggctc ccccacacag cccgccataa atcgcgaatg
      601 tgactatgca aacgatatga aaaaagacgc gggcgcgtct catatcgctc cgtaaacatc
      661 cggggtgcta atcccgacgc cagattttgc tggcgcgtga ggaatatagc cccggataac
      721 agattgagtc aacaaacggt ttttagatcc ccggaagaga atgcatcacg catcggtaga
      781 tagagcatat attcagcaag atttagccat acatctaccc gactgcagca cgtccggaa
//
LOCUS       DADLSE010000379          823 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLSE010000379 DADLSE010000000
VERSION     DADLSE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.383"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            29..>823
                     /gene="ltrA"
                     /locus_tag="JN692_004420"
     CDS             29..>823
                     /gene="ltrA"
                     /locus_tag="JN692_004420"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7266483.1"
                     /translation="MKDRNGSGAKGLPHCADGAAATTGDNADGRTAVKSAKPFPVSKR
                     QVWEAYKRVKANRGAAGIDGQTLAGFDENVTDNLYKLWNRMASGSYMPQAVRRVDIPK
                     ADGGVRPLGIPAVSDRIAQMVVKQILEPVLEPLFHADSYGYRPGKSAHQAIAQARKRC
                     WKFDWVVEVDIKGFFDDIDHHLLLKTVQHHTQARWVVMYIERRLKAPVQMPDGAMLAR
                     GRGTPQGGVISPLLSNLFLHYAFDMWMQRQFPGVPFERYADDVVCHC"
BASE COUNT          195 a          197 c          256 g          175 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatca cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgt
//
LOCUS       DADLSE010000380          819 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLSE010000380 DADLSE010000000
VERSION     DADLSE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.384"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..153
                     /locus_tag="JN692_004421"
     CDS             <1..153
                     /locus_tag="JN692_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266484.1"
                     /translation="YWFLQLLDRVSPSQWAAIGVLGSLLFGLLTYLTNLYFKIREDRR
                     KAARGE"
     gene            158..502
                     /locus_tag="JN692_004422"
     CDS             158..502
                     /locus_tag="JN692_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HAY7266485.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPIILEAQNVWITYKGKLPG
                     RITDSLKTPPKW"
     gene            complement(468..740)
                     /locus_tag="JN692_004423"
     CDS             complement(468..740)
                     /locus_tag="JN692_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266486.1"
                     /translation="MDNNTISLQELLDCISRLRDDVNALTVAFSSLALSIPREQMQPT
                     LASLLLESRNPKWSQEQQNSFKWLAALLEEKYAGKITISVESSENQ"
BASE COUNT          217 a          164 c          220 g          218 t
ORIGIN      
        1 tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc aataggcgtg
       61 ctggggagtc tgctgtttgg gctgctgaca tatctgacta acctgtattt caaaatcaga
      121 gaggaccgtc gtaaggctgc acggggagag taattcaatg actcaaaact atgaactgat
      181 tgtgaaaggg atccgcaatt ttgagaataa agttacggta actttagcgt tacgggacaa
      241 aaaacgcttt gacggtgaaa tttttgacct ggacatctcg ctggaccgtg ttgaaggtgc
      301 cgcgctggag ttttatgagg cagcagccag aatgagcatc agacaggtct tcctggatgt
      361 tgctgccggg ttatgtgaag gggatgagca gtcgccggaa aagcgcccca taattttaga
      421 ggcgcagaat gtgtggataa cctacaaagg aaagctacca ggaagaatta ctgattctct
      481 gaagactcca ccgaaatggt aattttacca gcatattttt cttccagtaa tgccgccagc
      541 cacttgaaag aattttgttg ttcctgggac catttggggt tgcgtgattc aagcaggagc
      601 gatgccagtg ttggttgcat ttgttctctg ggaattgata aggccagaga tgaaaatgca
      661 acagtgaggg catttacatc atcccgaagc ctggaaatgc agtcgagcaa ctcctgtaga
      721 gaaatggtgt tattgtccat aaataatcct cttgattgtc tttacctttt cccagcctga
      781 ttcaacaggc tgggacagat aaacatatcc agggttcag
//
LOCUS       DADLSE010000381          815 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLSE010000381 DADLSE010000000
VERSION     DADLSE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.385"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..251)
                     /locus_tag="JN692_004424"
     CDS             complement(<1..251)
                     /locus_tag="JN692_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266487.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIA"
BASE COUNT          215 a          206 c          163 g          231 t
ORIGIN      
        1 gcaatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag ttttttgttt
       61 ccggcatcca caaagaacgg aatattcagg tctgcgcaac actcaacggc atcgtcaaaa
      121 ttatcaaagc gcagaacctc agcttcttct tcgtcaaaaa aatcatcttc gtgaagtttt
      181 acgacatagc ggaggagttt gcttctttga ggtgcaggtt ttcgtttgcg tggtttagct
      241 gaacgggcca tataactacc tgaaagacaa tgatattgtc tgtttttata acggtaatca
      301 caggccatga caagccgcag ccgtcaggcc gcctactcgg ggcggtttag aacgcttatt
      361 tagtaccaga tcttacttat aataaagggg cttggcagtt tggataaact attgtcctta
      421 ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca tactgaaatc
      481 cccggaagta ttcaatcaaa caattacacc ataaaaggaa gaggggcccc aattaagttg
      541 tcaagcatgt ttactgcgca atcgcttggt gctccgtccc tgtaccccgc attggtattc
      601 tggcgctcat ggggcttcca cttgcgcttc tcccttcaca tcggggtgac aggtccacgt
      661 tccccacaag agcctgaatc gacttcacgc cgcttttatg ccggatgccg cgcaggcagt
      721 aaacatatcc cacaggaacg actccaccat tgttttgaca tcatctatat gctttcgaca
      781 catcatcagc ggttcacttt cgttcgtctc ttcga
//
LOCUS       DADLSE010000382          788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLSE010000382 DADLSE010000000
VERSION     DADLSE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.386"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(154..666)
                     /locus_tag="JN692_004425"
     CDS             complement(154..666)
                     /locus_tag="JN692_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7266488.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
BASE COUNT          238 a          164 c          178 g          208 t
ORIGIN      
        1 tcagcgacca gacagcattg agaatgcttc aggagtacat caggatccaa tgccttgggg
       61 ggtagcggta attttactca ttatccttca aatcagattc tgttgtcaga ggaatggggg
      121 aagctggatt cgcaagagct ttttatgggc agtttagcta aaaaaaggca acacgaagct
      181 aaaactacca acataaagtg ctgaccagct agcagctgaa atcagactca ctgtatacac
      241 tttcactgga tgaagttgca acattcctgc caccaaagga gcaatgtaac gcagcacggc
      301 gataaagcgc gaagtgaaaa gtattgacac agagttattt tgcaactgaa ggcgaacccg
      361 ttctattgta ttggaacggc tcgatattat acatgcaagt tgagggatat gcccaattat
      421 tataccaaga tggtaattaa cgatagtacc gcaccatgcg ccactcatga ccgtgattcc
      481 agcttcccat ggtgaaagag tcgtcttgct gactgtaatt acagtggcca tcataattga
      541 tgctggtggc aatatcgccg aaatgagtaa agtggatttt gtgaacgcta ttataaaaag
      601 aagcccccat aggcgatatg gatgaagtac aaaatagttc atcaaagcat ttatccactc
      661 catcaatgtc tttccttctg tgaatttttc tttgctattt aaccgtgata cgacttaatt
      721 taggcttaaa caccttacat ttacaaggta ctcctgtggg gggccctgcc acggagcgtc
      781 gcaggagt
//
LOCUS       DADLSE010000383          770 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLSE010000383 DADLSE010000000
VERSION     DADLSE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.387"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>770
                     /locus_tag="JN692_004426"
     rRNA            <1..>770
                     /locus_tag="JN692_004426"
                     /product="23S ribosomal RNA"
BASE COUNT          217 a          169 c          236 g          148 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg
      661 gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat
      721 agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca
//
LOCUS       DADLSE010000384          763 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLSE010000384 DADLSE010000000
VERSION     DADLSE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.388"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>763
                     /locus_tag="JN692_004427"
     CDS             <1..>763
                     /locus_tag="JN692_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7266489.1"
                     /translation="YLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLK
                     GIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIK
                     MADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMVEVNLYKVIAPYVNLFK
                     ADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNM
                     ANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFD"
BASE COUNT          252 a          133 c          120 g          258 t
ORIGIN      
        1 ttaccttagt aaagaaaagg atgtatccat taaggtggta tattttataa ataatgtcgc
       61 cgtccataat aatactatcg aaattccaca gatagtaaat ggtggttacg atttttcaca
      121 ccttagcctg aagggtatcg tgattaaaga tgaagattta tccaattcga attttgcagg
      181 ttgcagacta caaaacgcta ttttccagga ctgtaatatg tataaaacga atttttatta
      241 tgccataatg gaaaaaatac tttttgataa ttgtattctc gatgactcaa atttcgctca
      301 gataaaaatg gccgacggaa ctctaaatgc atgctccgct atgcatgttc aattctacaa
      361 tgcagcaatg aatagagcca atattaaaaa taccttcctt gactattcaa atttttatat
      421 ggcgtacatg gttgaggtaa atctttataa agtaatagcg ccatatgtta atttatttaa
      481 agccgacctt agtttctcta aactcgattt aattaacttt gaacatgctg atctgtctcg
      541 cgtcaatctg aacaaagcaa tcctccagaa tataaactta attgatagca aactcttttg
      601 tacgtggctg acaaatacat tcctcgaaat ggttatatgt accggctcta atatggctaa
      661 tgttaatttt aataatgcca atttaagcaa ctgccatttc aactgttcta ttttaacaaa
      721 agcctgtatg tttaataccc gtctctatcg ggttaatttc gat
//
LOCUS       DADLSE010000385          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLSE010000385 DADLSE010000000
VERSION     DADLSE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.389"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..131
                     /locus_tag="JN692_004428"
     CDS             <1..131
                     /locus_tag="JN692_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7266490.1"
                     /translation="FLTGAAFSLVMPFLPLYVEQLGVTGHSALNMWSGIVFSIMGS"
     gene            155..>755
                     /locus_tag="JN692_004429"
     CDS             155..>755
                     /locus_tag="JN692_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7266491.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAF"
BASE COUNT          204 a          150 c          188 g          213 t
ORIGIN      
        1 gttttcttac cggggccgcc ttcagtctgg taatgccctt cttacccctc tacgttgagc
       61 agcttggtgt taccggccac tccgccctga atatgtggtc cggtattgtc ttcagtataa
      121 tgggttccta aacacatcat tttaggtatt gaatatgaga cgggcaatca gctacatcag
      181 attttcaagt gaacgacaac tgaaagggga ctctgtacgc cgacaatcta agctggttac
      241 tgactggctt gataagaacc ctgagtttta cttggattca tccttgagct ttaaagattt
      301 ggggaaaagt gcttttagtg gtaagcacct gaaaggtggt ctaggtgact ttttaaccgc
      361 tatcgagaaa gggcttgtta aagctggtga tacgctacta atcgagagtt tagaccgtct
      421 gtcacgtcag gatattgata ttgcttctga gctattacgc cgtatcttaa gggctggtgt
      481 agatgtagtc acactgagcg atggagaaca ttacacacga gaaagtttaa aagacccgtt
      541 agccctgatt aaaagcattc tgataatgca gcgggcgcat gaggaaagtt tgaggaagtc
      601 tgagcgtgtc caagctgcat ggaaccgtaa aaaagaactc atctccgaag gtatcaaggt
      661 atctaggcgt tgtcctgcat ggttaaggct gaatgatgac cgtcgaacat tcacaatcat
      721 tcctgataaa gtggaagttg ttaaacgagc atttg
//
LOCUS       DADLSE010000386          755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLSE010000386 DADLSE010000000
VERSION     DADLSE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.390"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JN692_004430"
     CDS             complement(<1..491)
                     /locus_tag="JN692_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7266492.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKH"
     gene            complement(638..714)
                     /locus_tag="JN692_004431"
     tRNA            complement(638..714)
                     /locus_tag="JN692_004431"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(678..680),aa:Pro,seq:tgg)
BASE COUNT          186 a          192 c          188 g          189 t
ORIGIN      
        1 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
       61 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
      121 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
      181 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
      241 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
      301 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
      361 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
      421 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
      481 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
      541 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
      601 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
      661 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
      721 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLSE010000387          754 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLSE010000387 DADLSE010000000
VERSION     DADLSE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.391"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(673..747)
                     /locus_tag="JN692_004432"
     tRNA            complement(673..747)
                     /locus_tag="JN692_004432"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Gly,seq:tcc)
BASE COUNT          231 a          181 c          159 g          183 t
ORIGIN      
        1 ccgacagacc tgtgctatcg gaaagaaaaa aggccatcag caaaactctg atggcctgaa
       61 tcacctttac caatattgta tgaaaaaaca cgcacgacta attgacaata attttcattt
      121 ccattaaaaa agatcgcgta gcattcttaa ttcatgagga actttacccg ccagcaatct
      181 gagtagcgaa agctgttcat ccccaatgtt tttgctggcg ggtccttttt tctacggtcc
      241 tcctccccgg aggggggggc atttcattat ttttctaatc gttactggtg agaaggcgga
      301 agaaaaagcc agttcttcgc ccctaaataa cagcatgatt ccagtccatg caaaatctga
      361 tttccagctc acacataccg agcactgtac aaaaaaccgc cagccaaggc agttaagagt
      421 gtgttcccgg ggtttgctta gaatattttg taagttgtcc gaagagatat ttacaacacc
      481 agaatgatgc attatcagtc ccctgccaga ggttcactac gtaacctgac cgacaatgca
      541 ccattctggt gctgaaaaaa gagcactgga actgcaacaa aacaacatca cagaacagga
      601 gatggtaagg agtcagacat tcccacacaa tattgtgtca gtgcaaataa caaccttcgt
      661 ctcaaatctc gctggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      721 aggtaatagc cattatacga tgcccgcata tggt
//
LOCUS       DADLSE010000388          749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLSE010000388 DADLSE010000000
VERSION     DADLSE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.392"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..342
                     /locus_tag="JN692_004433"
     CDS             <1..342
                     /locus_tag="JN692_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7266493.1"
                     /translation="AKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRR
                     GIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIAR
                     VRSVEEILHAL"
BASE COUNT          218 a          176 c          167 g          188 t
ORIGIN      
        1 gctaaagtgt tgcccgaaaa ctggtggcaa catccagctg cattaggtgc aactgacagt
       61 gatatcgaaa tcatcaaacg tcagtggggt gcgttttacg gtacggatct ggagttgcaa
      121 ctgcgccgcc gaggtatcga tacaatagtg ttatgtggga tctcgaccaa tatcggtgtt
      181 gaatccaccg cccgcaatgc ctgggaactt ggctttaatc tggtgattgc cgaagacgcc
      241 tgtagtgccg ccagcgccga gcaacacaat aacagcatta atcatatcta cccgcgcatc
      301 gcccgtgtgc gtagcgtgga agagatcctt cacgcgttat gatttacatc ggtctaccgc
      361 aatggtcgca tcctaaatgg gtgcggttgg ggatcaccag ccttgaagag tatgcccgcc
      421 actttaactg cgtgatacgg gatttttaaa aatcactaaa gaacgcccaa gagcatgtgt
      481 tttctttaga ataatcaaag cgttggattg ccatgtcata tgaaaatcgg caaagttcat
      541 atgtaacaaa gtgtcccaat catgccccaa aatgccccac aacaaatatt tttgccccat
      601 gcatgcccca aaaaatcact ttccctcact cttgacactg tttatccata cagttaaaaa
      661 taatactgta taaaaacaca gtgtagaggg gtttttatgc gtattgaaat ctgcatagcc
      721 aaagagaaaa tgactaaaat gccaaccgg
//
LOCUS       DADLSE010000389          748 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLSE010000389 DADLSE010000000
VERSION     DADLSE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.393"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN692_004434"
     CDS             complement(<1..538)
                     /gene="rclC"
                     /locus_tag="JN692_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HAY7266494.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAR"
     gene            complement(550..741)
                     /locus_tag="JN692_004435"
     CDS             complement(550..741)
                     /locus_tag="JN692_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7266495.1"
                     /translation="MAFSTNADDKIILKHISVSSVSASPTVLEDAIADIARKYNASSW
                     KVTSMRIDNNSTATAVLYK"
BASE COUNT          215 a          176 c          155 g          202 t
ORIGIN      
        1 cccgcgccga atctgccatt atcatgacag cacctgccag catcagagta tctttcaata
       61 ccaaacgacc agcaccggat aaataaggga aaccatgatg agcgtcaccc aatgcgggta
      121 cccatgcctc cggggtggtg attaaaaatg agagtgttac cagcggcgtg gtaaatgcca
      181 tcagtcctcc caataaacct aaccagcgat tgacaggatt agccaaaacc agcagagcaa
      241 taatcacctc cacgacgcca agaccgttgg aaaaaccata ggtattattg gccgtttgcc
      301 atgcccttgc ttctggtttg tattcgcctt cgtgagtcag atactgttta tagtcttccg
      361 ggtgttcata aaagaacgac attagtggac tgtttgcgac gaatggtgta atgctgtctg
      421 cctcgtaagg gacaaacttt aataacccaa tccacataaa aacaattgca atactcagac
      481 gaatcaatgt caggccaatt ttatctcccc gacttaacag gtgcaggtat ttttccattg
      541 tgaacatcct tatttataca atactgctgt tgcggttgaa ttattatcaa ttcgcatcga
      601 tgtgactttc caggatgaag cattatattt tctggctata tcagcaatgg catcctccag
      661 aactgtcggt gatgctgata ctgacgagac gctgatatgt ttcagaatta ttttatcatc
      721 tgcattggtg gaaaatgcca taacgcca
//
LOCUS       DADLSE010000390          740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLSE010000390 DADLSE010000000
VERSION     DADLSE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.394"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(175..>740)
                     /locus_tag="JN692_004436"
     CDS             complement(175..>740)
                     /locus_tag="JN692_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAY7266496.1"
                     /translation="IEKAHHDVHELFFQVFDKGAMEDGEGVHIDFKNTTLLLTTNAGA
                     ELISQLCEDPTLMPDSAGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETARGVIARL
                     HLGRLMDRMAEQHGVSLVYGDELITHIVSRCPMHETGARLLIGYIEQHILPQLSRYWL
                     QAMTEKTAITQIRIRVDDDEQVIFETD"
BASE COUNT          194 a          202 c          172 g          172 t
ORIGIN      
        1 gggagggcga tatgttacag agatactgac ccttgcacgc gcattaccaa gcgcgcgcaa
       61 aaaattatct actgatgcac gaaaagtccc ctgcaaatca ttagtggaag aacttccagg
      121 aaagcgactg acccacagtg cgccacttaa ttcttttgcc ataaggatgc tccattaatc
      181 cgtttcaaaa ataacctgtt catcgtcatc aacacgaatg cggatttgcg tgatggccgt
      241 tttctctgtc atcgcctgca accagtaacg ggacagttgt gggaggatat gttgttcgat
      301 atagccgatg agcaaacgag cgccggtttc gtgcatcggg cagcgcgaca caatatgcgt
      361 aatcaattca tcgccataca ccagtgaaac gccatgctgc tccgccatcc gatccatcaa
      421 tctgccaaga tgcaggcggg caataacacc acgcgcggtc tcatccagcg gtaaataagg
      481 aataaccgtc acacgcccca ggaaagccgc cgggaagtgt ttacgtaact ccggcattaa
      541 cgcctctttt agtcctgccg aatcaggcat cagcgtcgga tcctcacaga gctggctgat
      601 aagttccgct cccgcgttag tagtcaacaa cagcgtggta tttttgaaat cgatgtggac
      661 accttcgcca tcttccatcg cccccttatc aaacacctgg aagaacagct cgtggacgtc
      721 gtgatgggct ttttcaattt
//
LOCUS       DADLSE010000391          729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLSE010000391 DADLSE010000000
VERSION     DADLSE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.395"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(247..>729)
                     /gene="sseA"
                     /locus_tag="JN692_004437"
     CDS             complement(247..>729)
                     /gene="sseA"
                     /locus_tag="JN692_004437"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7266497.1"
                     /translation="GLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASH
                     ENTPQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIF
                     FGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK
                     "
BASE COUNT          185 a          199 c          161 g          184 t
ORIGIN      
        1 ttcggactaa taagccaggt tattttatta ccgaacacgg cggaaagggc cggtgtcgat
       61 aatacgctaa ataaacaata attactctct tttgcttgac aaaaagagag ttactggtga
      121 gtattgtttt gctgtgaagc acctgcgttg ctcatgtaac acaagtcaac ctgctggaaa
      181 ggccgcgaac cagaccagca ataaaaaaac gccaaattcg gcggtttttt attgcctgta
      241 aaatacttat ttcactggct caaccggtaa atctgcccgc gcgccccatt cactccatgc
      301 gccgtcgtac agtttcacgt ttggcacatc cagcgtcgcg agtgctaaca aaaccacggc
      361 tgccgttaca ccagagccgc agctgacgat aatcggtttg tcatagctga cgccgcgacc
      421 aaaaaatatc gcatccagtt catcggtcgt ttttagttcg ccttcgcgca ccagttccgt
      481 ccacggaacg ttcaatgcac cgggaatatg tccgcgacgt aaacctgggc gaggttcatc
      541 aacttctgcg ttaaaacgtg cagccgggcg ggcatcaata atttgcggcg tattttcatg
      601 gcttgccaat aacacatcgg ttactttcac cactgcttca ggattaaacg cggcgttaaa
      661 ctctccttcc ggcagctcta ctgcaccttc ttctaatagc aggtcatcgc gctgccagcc
      721 tgcaagtcc
//
LOCUS       DADLSE010000392          728 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLSE010000392 DADLSE010000000
VERSION     DADLSE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.396"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(282..356)
                     /locus_tag="JN692_004438"
     tRNA            complement(282..356)
                     /locus_tag="JN692_004438"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(321..323),aa:Gly,seq:tcc)
     gene            complement(360..435)
                     /locus_tag="JN692_004439"
     tRNA            complement(360..435)
                     /locus_tag="JN692_004439"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Thr,seq:tgt)
     gene            complement(438..514)
                     /locus_tag="JN692_004440"
     tRNA            complement(438..514)
                     /locus_tag="JN692_004440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Arg,seq:tct)
     gene            complement(516..592)
                     /locus_tag="JN692_004441"
     tRNA            complement(516..592)
                     /locus_tag="JN692_004441"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Ile,seq:cat)
BASE COUNT          214 a          180 c          173 g          161 t
ORIGIN      
        1 cgcaaataac ggatggcgta gttttacact gagaaatgaa agggatttga aaagaaaaaa
       61 acgcaaaagc gggcgaaacg atatatacag taaggaaagc actctatcca acaaaccacc
      121 cacagttaat cggaataaaa gcagagtgct tatgaatgat cgcacgcccg aaggttagta
      181 tttctgcaca gcaattttgc aaaaaaaggc gatcattcat aacttaaacg tctttcagtc
      241 actccgggat ttcccatcat cgcagactga aagactctga ctggagcggg cagcgggaat
      301 cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat gcccgcatat
      361 ggtgccgact accggaatcg aactggtgac ctactgatta caagtcagtt gctctgcctg
      421 ctgagctaag tcggcgctgg cccaccaccg aggactcgaa cctcgcaccg tcaacttaga
      481 aggttgatgc tctatccgga tgagctagtg gtggttggtg gcccttgctg gacttgaacc
      541 agcgacctgg cgattatgag tcgctcgctc tgaccaactg agctaaaggg ccggaggcag
      601 aataataacc atatgtcatc acatctgcaa actcatctga ccaccagcgc gtttaacgcc
      661 ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca agtgtccatg
      721 tctgctat
//
LOCUS       DADLSE010000393          724 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLSE010000393 DADLSE010000000
VERSION     DADLSE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.397"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..517)
                     /locus_tag="JN692_004442"
     CDS             complement(<1..517)
                     /locus_tag="JN692_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HAY7266498.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSR"
BASE COUNT          202 a          169 c          176 g          177 t
ORIGIN      
        1 ccctcgagcc gctatgaacc agcagttgca gatgctgcgc atccagacct gccagtgcaa
       61 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccaccac
      121 cgatagagcc aagcgagctg cgccacggat gctgtgcaaa tgctgacggc aggttttcct
      181 ccagccagct ttcttcagca acgtcatcca aagctgataa tcgcttttca aacttatcgg
      241 cgttgtattt gcgagcgaga atatctgttt gccatagcgc catgccacag ccgatatcat
      301 tgccgaccag tgctgggtaa aaacggcctg cggagaagaa cgctgcgcca atcgggtagc
      361 cgcgtccggg gtgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
      421 tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa
      481 ataccgcatc ggataaggga cgaatataat tgcccatata ccattccgta aatagactcg
      541 tcatacttca agttgcatgt gctgcgactt gaattattga gagtaacaat taaaaaatca
      601 ggaaatggca gacgaatcca tggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
      661 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
      721 gcgc
//
LOCUS       DADLSE010000394          722 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLSE010000394 DADLSE010000000
VERSION     DADLSE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.398"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..451
                     /gene="ospC3"
                     /locus_tag="JN692_004443"
     CDS             <1..451
                     /gene="ospC3"
                     /locus_tag="JN692_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HAY7266499.1"
                     /translation="DNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLG
                     NKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINN
                     GLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          253 a          119 c          143 g          207 t
ORIGIN      
        1 agataacttc aaaaaaataa aactcagaga catatctttg gaggatgcga ttaaagcatc
       61 taattatgaa gagattaaca ataaagtgac tgataaaaaa atggctcatc aggcccttgc
      121 ttattcttta ggcaataaaa aagctgatat agcactttat ttactatcta aatttaattt
      181 tacaaaacaa gatgtcgcag agatggagaa aatgaacaac aatagatatt gtaatctgta
      241 tgatgttgaa tacttactta gtaaagatgg tgccaactat aaagtgctag aatattttat
      301 caataatgga ttggttgatg taaacaaaaa atttcaaaaa gcaaatagtg gggacactat
      361 gcttgacaat gcaatgaaaa gcaaagattc aaaaatgatt gattttttat taaaaaatgg
      421 agcaatatta ggcaaacgat ttgagatatg acctaaaagc tcggccaaat aataatgtcg
      481 tcgattatcg aagtcatcat gagtgattct gtccccacag tgcagcatca gcatgacagt
      541 gaattgtctg gcgtggtcca tttaatgaga tggagatggt ccctctttcc taaatagctg
      601 tgcctaatac cgctacactt ttgccagccc atgtttgcct ccggggaatg ggctgacggt
      661 tccttcaaaa tccattgcac gcagttatgt cggagcggga acttgactcc tgtctttacg
      721 gg
//
LOCUS       DADLSE010000395          682 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLSE010000395 DADLSE010000000
VERSION     DADLSE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.399"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..682
                     /locus_tag="JN692_004444"
                     /pseudo
     CDS             1..682
                     /locus_tag="JN692_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          168 a          176 c          185 g          153 t
ORIGIN      
        1 aacaggttgt tgatcgcggt tattctgttg ccagcgttgc aacacgtctc gatatcacca
       61 cccacagcct ttatgcctgg ataaagaagt acggtccgga ttcttccact aataaagaac
      121 agtcagatgc tcaggccgag atccgccgtc tccagaaaga gctgaaacgg gttaccgacg
      181 aacgggacat attaaaaaaa gccgcggcgt acttcgcaaa gctgtccgac tgaggtacgc
      241 ctttatccgt gacaactcct gttgctggcc tgttcgcctg ctctgtcggg tgctggatgt
      301 tcatcccagt ggtttttacg cctggcttca gcagccgcat tcacaacgcc atcaggcaga
      361 cctgagactg acaggacaga ttaaacagtt ctggctggaa tcgggatgcg tctatggtta
      421 tcgcaaaatc catctggatc tgcgtgacag cgggcaacag tgcggagtaa acagagtctg
      481 gagactgatg aaacgtgtcg gaataaaggc tcaggtcgga taccgaagcc cgcgggcacg
      541 taaaggcgag gccagtatcg tgtcacccaa caggctccag cgacagttca atccggatgc
      601 tcctgatgag cgttgggtaa cggacataac ctacatcagg acccacgaag gctggctgta
      661 tcttgccgtt gttgttgatc tg
//
LOCUS       DADLSE010000396          677 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLSE010000396 DADLSE010000000
VERSION     DADLSE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.400"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..567
                     /locus_tag="JN692_004445"
                     /pseudo
     CDS             1..567
                     /locus_tag="JN692_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          170 a          159 c          228 g          120 t
ORIGIN      
        1 ggaaacgggg gcaggaaccc ggtatcttct gcgccatcca cacgtatggc cgtcgtctca
       61 actggcatcc gcatgtacat gtcgaaaaaa acggccggcg ggaggaatac ggcgcgctac
      121 ctgggtcgtt atctgaagaa gccgccgata gcggcctccc ggctggcaca ttacaacgga
      181 ggggcgagcc tgagcttccg ttacctggac cacaaaacgg gagaaacggc gacggaaacg
      241 ctgacacagc gtgagctggt cgcgaggctg aaacagcaca tcccggagaa gttttttaag
      301 atggtgaggt acttcgggtt ccttgccaac cgggtgtgtg gagagaagct gccgcaggtg
      361 taccgtgctc tggggatgga taaaccggaa ccagtggcga aggtgtgcta tgcacaaatg
      421 gtgaagcagt tcctgagtcg tgacccgttc gaatgcgtgc tgtgtggcgg ccggatggta
      481 taccgccggg ccattgcggg gctgaatgtg gaggggctga agaaaaacgc gcgggatatc
      541 agtctgctga ggtatatgcc ggcctgacac aggctaagtg tgcccgggaa tcggtgcaag
      601 tataaaaaac agcctgttgc ggtataagaa tcgaccatgc agagagagaa aacaccggag
      661 tggagggaaa agcagaa
//
LOCUS       DADLSE010000397          666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLSE010000397 DADLSE010000000
VERSION     DADLSE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.401"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            163..237
                     /gene="tnaC"
                     /locus_tag="JN692_004446"
     CDS             163..237
                     /gene="tnaC"
                     /locus_tag="JN692_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HAY7266500.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            458..>666
                     /locus_tag="JN692_004447"
     CDS             458..>666
                     /locus_tag="JN692_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266501.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRG"
BASE COUNT          208 a          122 c          125 g          211 t
ORIGIN      
        1 ttttaaacat taaaattctt acgtaattta taatctttaa aaaaagcatt taatactgct
       61 ccccgaacga ttgtgattcg attcacattt aaacaatttc agaatagaca aaaattctga
      121 gtgtaataat gtagcggcgt gtctttcgag gataagtgca ttatgaatat cttacatata
      181 tgtgtgacct caaaatggtt caatatcgac aataagattg tcgatcaccg cccttaattt
      241 acccttctgt agccatcacc agagccaaac cgattagatt caatgtgatc gattagtttg
      301 ctatatctta attttgcctt ttgcaaaggc catctctcgt ttatttactt attttagtaa
      361 atgatggtgc ttgtatatat acatggcgaa ttaatggata ttgcagatgt aatattcaca
      421 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
      481 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt actactcgcg cttatcgtga
      541 agaagcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat
      601 cgatttactg accgacagcg gcaccggggc ggtaacccaa agtatgcagg cagcgatgat
      661 gcgcgg
//
LOCUS       DADLSE010000398          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLSE010000398 DADLSE010000000
VERSION     DADLSE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.402"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..566
                     /locus_tag="JN692_004448"
                     /pseudo
     CDS             1..566
                     /locus_tag="JN692_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          169 a          136 c          161 g          192 t
ORIGIN      
        1 gctggtttga gttacaaaga ttttggttca ttcgactaca gtcgtaatgt cggtgtcgcg
       61 tatgatgcag aagcatttac tgatatgttt gttgagtggg gcggtgactc atgggctggt
      121 actgacctgt tcatgaccaa ccgtaccaac ggtgttgcaa cctatcgtaa cacagatttc
      181 tttggcatgg ttgaaggtct aaattttgct ctgcaatatc agggtaaaaa tgaaggtaca
      241 ggcaactaca aagcaaatgg cgatggccat ggtttatctg caacggtttc tcatttgctg
      301 gtgcttatgc aaactctgac cgtactgatt ggcagtctgg tgatggtaaa ggcgaaagag
      361 ccgaagtttg ggcgttgtca acaaaatatg acgctaacaa tgtttatgct gctgtgatgt
      421 acggtgaatc tcacaacatg aattccgacg acggtgatgt tgtgaacaaa acacaaaact
      481 tcgaagctgt tctgcaatat cagttcgact ttggtctgcg tccatccatc ggttactcct
      541 attctaaagc actggacgta gcaggttgga tttgccccta tatttccaga cacctgttat
      601 aacttaaccc attactggct tgctgccgta gatattcccg tggcgagcga taacccag
//
LOCUS       DADLSE010000399          653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLSE010000399 DADLSE010000000
VERSION     DADLSE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.403"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            220..>653
                     /locus_tag="JN692_004449"
     CDS             220..>653
                     /locus_tag="JN692_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266502.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGAL"
BASE COUNT          150 a          144 c          159 g          200 t
ORIGIN      
        1 gcctaaaaca acaatatgga catcttaacg atactctgcg cctgcgtgtg cggaacagaa
       61 acccttaccc gatatacttg ttatgccaga ggtagcaatt gcgttagcgg cgtactgttg
      121 ctgtaaagta taacgcctat attttacagt tcagtcctct tttgcttatt acaaccttaa
      181 gtatatcaag catataaaga taataagaga ctgaacaata tggttacctg gacccaaatg
      241 tatatgccga tgggaggact ggggctatcc gctctggtcg ccctgatccc gataatattc
      301 ttcttcgttg cactcgcggt attacgtctg aaaggacatg tcgctggagc aataaccctt
      361 atattatcta tcctgattgc gatattcgcc tttaaaatgc cgattgatat ggcatttgct
      421 gctgcgggct atggctttat ttatggatta tggccaattg cgtggattat tgtcgcggcg
      481 gtgttcctgt ataaattaac cgttgccagc gggcagttcg atattatccg cagctcggtt
      541 atctccatca ccgacgatca gcgtttgcag gtgttactga ttggtttctc ctttggtgcg
      601 ttgctggaag gagcggctgg ctttggtgcg ccggtggcga ttaccggtgc gct
//
LOCUS       DADLSE010000400          653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLSE010000400 DADLSE010000000
VERSION     DADLSE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.404"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..578)
                     /locus_tag="JN692_004450"
     CDS             complement(<1..578)
                     /locus_tag="JN692_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7266503.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA"
BASE COUNT          167 a          164 c          155 g          167 t
ORIGIN      
        1 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact cagggctgga
      301 gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac cagttttcgt
      361 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat caaatcccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      481 tcacctattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      601 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taa
//
LOCUS       DADLSE010000401          621 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLSE010000401 DADLSE010000000
VERSION     DADLSE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.405"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..213
                     /locus_tag="JN692_004451"
     CDS             <1..213
                     /locus_tag="JN692_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266504.1"
                     /translation="PQDVQHFLSNAEMCQHLAGEWDSSLPEKDKRDIEKGVDTWCPPA
                     KKSLPVLREKYKENKEIIKILSAYDF"
     gene            275..>621
                     /gene="tssB"
                     /locus_tag="JN692_004452"
     CDS             275..>621
                     /gene="tssB"
                     /locus_tag="JN692_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HAY7266505.1"
                     /translation="MAINNSAQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMAVLA
                     DLAGKPREELPPVTDRKFLDIDIDNFNERMKAIAPRVAFAVPNTLTGEGQLMVDITLE
                     NMDDFSPAQIARKV"
BASE COUNT          168 a          142 c          170 g          141 t
ORIGIN      
        1 ccacaggatg tgcagcattt tctgagtaat gcggaaatgt gccagcatct tgctggtgaa
       61 tgggacagtt ctttaccaga gaaggataaa agagacatcg aaaagggggt tgatacctgg
      121 tgtcctccgg cgaagaaatc actccctgtg ctgcgggaaa agtacaaaga aaacaaagag
      181 attataaaaa tactctctgc gtatgatttt taaactgtac cgtcggacca ctccccaggt
      241 ggtcgtcggc tgactgataa ggaaatgaag gattatggct atcaacaaca gcgcgcagaa
      301 gtttattgcc cgcaaccgcg cgccccgcgt gcagattgag tatgacgttg agatttacgg
      361 ctccgagaaa aaaatcgaac tgccgtttgt catggcggtg ctggccgatc tggccggtaa
      421 accacgcgaa gagctgccac cggtcaccga tcgtaaattc cttgatattg atatcgataa
      481 cttcaacgag cggatgaaag ccattgcgcc gcgcgtggcg tttgctgtcc ccaatacgct
      541 gacgggcgaa gggcagttaa tggtcgatat cacgctggag aatatggacg atttttcgcc
      601 tgcgcagatt gcccgtaagg t
//
LOCUS       DADLSE010000402          616 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLSE010000402 DADLSE010000000
VERSION     DADLSE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.406"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN692_004453"
     CDS             <1..489
                     /locus_tag="JN692_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7266506.1"
                     /translation="EKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     FLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          181 a          124 c          138 g          173 t
ORIGIN      
        1 gaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tctgtacggc gtaaattcgg ggggcgctta cattcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactggggg tattaccttc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctataaag cgacattacc aattattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccgcttt tcccggttta tgatgaaaaa
      301 aaacatttcc tgactattga agttcggcca ccaatggatg catgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gtcacatccg
      421 gaacagtata tctgggtttt aaaattgtta aaaacgcgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaggtattt ccactagttg tttcttacag gttaccaatc
      541 gaaacacatc ccccttccgt gtgttgcccg tttggcacgg aggactgtgt agatgcagtt
      601 cgttaaattt aattta
//
LOCUS       DADLSE010000403          614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLSE010000403 DADLSE010000000
VERSION     DADLSE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.407"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>614
                     /locus_tag="JN692_004454"
     rRNA            <1..>614
                     /locus_tag="JN692_004454"
                     /product="16S ribosomal RNA"
BASE COUNT          152 a          139 c          200 g          123 t
ORIGIN      
        1 gcaagcttga gtctcgtaga ggggggtaga attccaggtg tagcggtgaa atgcgtagag
       61 atctggagga ataccggtgg cgaaggcggc cccctggacg aagactgacg ctcaggtgcg
      121 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgtcga
      181 cttggaggtt gtgcccttga ggcgtggctt ccggagctaa cgcgttaagt cgaccgcctg
      241 gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg ggggcccgca caagcggtgg
      301 agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc tggtcttgac atccacggaa
      361 gttttcagag atgagaatgt gccttcggga accgtgagac aggtgctgca tggctgtcgt
      421 cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga gcgcaaccct tatcctttgt
      481 tgccagcggt ccggccggga actcaaagga gactgccagt gataaactgg aggaaggtgg
      541 ggatgacgtc aagtcatcat ggcccttacg accagggcta cacacgtgct acaatggcgc
      601 atacaaagag aagc
//
LOCUS       DADLSE010000404          611 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLSE010000404 DADLSE010000000
VERSION     DADLSE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.408"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            31..393
                     /locus_tag="JN692_004455"
     CDS             31..393
                     /locus_tag="JN692_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7266507.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LRREMGLEEMTGFSKTTF"
BASE COUNT          192 a          141 c          138 g          140 t
ORIGIN      
        1 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
       61 cgtctcagcg atcgcctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
      121 gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc agaaattgct
      181 cgcctgcgtg aggttcatag ccaaaaactg agtaaagaag cacagaagct gatgaagatg
      241 ccgttccagc gcgcaattac caaaaaagag caggctgata tgggcaagct gaagaaaagt
      301 gttcgcggac tggtggttgt gcacccaatg accgcgctga ggcgcgaaat gggcctggaa
      361 gagatgactg gattttctaa gactacgttt taagcccctc agacgactgg ttcgatcgta
      421 tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt ctactcgcct
      481 aattaccaaa caattcactc cctttacttc gatttaccgc tggttgatat tctgaaaatg
      541 catttttacc gaaatagcca tctataaaca ataaaaatat atagaagata tcatttgtta
      601 attaacaagc a
//
LOCUS       DADLSE010000405          587 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLSE010000405 DADLSE010000000
VERSION     DADLSE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.409"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..491)
                     /locus_tag="JN692_004456"
     CDS             complement(<1..491)
                     /locus_tag="JN692_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7266508.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIMPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINA"
BASE COUNT          139 a          179 c          132 g          137 t
ORIGIN      
        1 gcattaatca caccctcagg cgcggatatc tccactttcc agggagatac aatccgtatc
       61 tggttgtcat caaataccac aaactggacg ggatcaccgt tgagcacgcc acccagccag
      121 atggcatcag cataattatg ggtacgcttt gatcccggca tcgcagcctg acgcgtggct
      181 tttaccgcac tgatatcccg atcgcagatg gcaagaaaac cgatatcccc cacatgtggc
      241 ggcataatca ccgcattacc gcccccctgt agtcgccata cgggaatgtt ataaatcacc
      301 tcatgttcaa ccggggaacc gtctgctgca acgcccatta ccatcggtcg ggcctcaata
      361 aatccctctt ccacggctac cacccgtccc agagtgataa acgcatgacg tccgagaaac
      421 tgccgcagca taaagtcctg tgcattgatt tcgctgttca cgtctgtcgg gttactgagt
      481 ggttgtgcca ttttcttcag ccctcatcat ggtacagttg gaactccacg gaccgcccac
      541 ggttcgcgaa gtaatggtgt gtatcacccc tgtgatcgta taatcgc
//
LOCUS       DADLSE010000406          577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLSE010000406 DADLSE010000000
VERSION     DADLSE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.410"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..209
                     /locus_tag="JN692_004457"
     CDS             <1..209
                     /locus_tag="JN692_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7266509.1"
                     /translation="LNALINSFNAALSPDSFISDFYGLIWNIDTAEKYGLDVWGKIVG
                     VSRRLTVKDDFNYLASASPGWTPR"
     gene            194..556
                     /locus_tag="JN692_004458"
     CDS             194..556
                     /locus_tag="JN692_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HAY7266510.1"
                     /translation="MDTPVMDDPCPFNQAPFYNGKSDTRTVDLSDAVYRRLILMKAMS
                     NITDCSVPDINRMLRFMFGKKRRAYVLNNGGLRMSYIFESALSLAELAIIQSSGALPS
                     PPGVYVSVVLKEPRNEGQ"
BASE COUNT          132 a          133 c          154 g          158 t
ORIGIN      
        1 gactcaatgc cctcattaac agctttaacg cagcgctttc ccccgacagt tttatcagtg
       61 atttttatgg tcttatctgg aacatcgata ccgcagaaaa gtatggtctt gatgtctggg
      121 gaaagattgt gggtgtcagt cgccggctga cggtaaagga cgattttaat tacctggctt
      181 cagcgagtcc aggatggaca ccccggtaat ggatgatccc tgtccgttta atcaggcacc
      241 gttttacaac ggaaaatcgg atacccggac tgttgacctg tctgatgctg tataccggcg
      301 gctgatactg atgaaagcca tgtcgaacat tactgactgt tccgttccgg atattaaccg
      361 gatgctgaga tttatgttcg gaaaaaaacg ccgggcttat gttctgaata atggtgggct
      421 gaggatgagt tacatctttg agtccgcgct ctcgttggca gaactggcga ttatccagtc
      481 gtcgggtgca ctgccatccc cgccgggtgt ttatgtttca gtggttttaa aggagccccg
      541 taatgaaggc cagtgataac ccccgccagc tggcggt
//
LOCUS       DADLSE010000407          573 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLSE010000407 DADLSE010000000
VERSION     DADLSE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.411"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..365)
                     /locus_tag="JN692_004459"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="JN692_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            288..539
                     /locus_tag="JN692_004460"
     CDS             288..539
                     /locus_tag="JN692_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266511.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          156 a          131 c          142 g          144 t
ORIGIN      
        1 gccagtgttg ttgcgccttg ctgccaacga agcgccactg ctcatcaagt tcacagataa
       61 gggcaacatc agcgtgagcg actggcgcag atgttatctg ccggggcgag agctttttaa
      121 tgtccgtatg acggtattga tgccgatttt cagtgttctg gcggtataac agccccctgc
      181 gccgttatgt gccatttcaa cgatctgctc cttaacgccg agtttctggc ttcataagag
      241 taagtgagct ggaaaacacg tttacatgag cgacaacgga aacgctcatg ccgggaacaa
      301 ctacggccat gtcgataaac ttcatcggaa ttacagcgcg gacaatgaac agtaacggta
      361 gtcatggaaa acctcaaata ccagtagatt accagaaact tctatcgatt gaagacatta
      421 ccttttgccg caataggtgg ggtaacattg gtgaaaacgc attacgccgg gtggcagttg
      481 gaaaaaaatt atcttttttc ggctctgatc gtggtggaga aaatgcggca ataatctaaa
      541 tagctgcgct taataccgct acacttttgc cag
//
LOCUS       DADLSE010000408          564 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLSE010000408 DADLSE010000000
VERSION     DADLSE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.412"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(172..>564)
                     /locus_tag="JN692_004461"
     CDS             complement(172..>564)
                     /locus_tag="JN692_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7266512.1"
                     /translation="HYAGQDEIGLRYNSHRTKREEYLLMSGDEFMERFSWHVADKGFR
                     MVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQM
                     RFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          135 a          183 c          106 g          140 t
ORIGIN      
        1 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctcc
       61 atatgaacgg aaccgacgat taaactggat ggctctgatt gattcagggt atgaatggcg
      121 gttttttgct ccgtttccct caaaatggac gcaacttccc ctctgcggct ctcagccgca
      181 ccaccgcatc cgggccagcg gctcatgcat caggacctgc tctgccagac ggtagccccg
      241 cttcagcccc gtaaaacgca tctgactccc gcacagcacg cacttcagcg ggtcaacctt
      301 cagtaacctc tgatacatcc ctctccaggt gatttgcatc gccgtttttc tcactgtctc
      361 cgttatgatg tacaccactt cttccagtaa ccgccgtttc gccggactca aaaaaccgta
      421 gtacctcacc atacggaacc ccttatccgc cacatgccag gagaaccttt ccatgaactc
      481 atctccactc atcaacaggt attcttcccg ttttgttcgg tgactgttgt aacgcagacc
      541 gatttcatcc tgaccggcat aatg
//
LOCUS       DADLSE010000409          559 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLSE010000409 DADLSE010000000
VERSION     DADLSE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.413"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            73..>559
                     /locus_tag="JN692_004462"
     CDS             73..>559
                     /locus_tag="JN692_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266513.1"
                     /translation="MLYAARKRGLEIGIFCTIHTLRLHFEEHLPLVVAGRRLGVPKST
                     VCSMFVRFRKAGLSWPLPAGMSERELDARLYGSASTVPVVLTESTVMPEVPGVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LL"
BASE COUNT          123 a          160 c          148 g          128 t
ORIGIN      
        1 gctatttaga acggcacgga accacggcag cttgacggct gcaatgacgt gtgtcgtctg
       61 gcggtggaga atctgctgta tgcagcccga aaacgggggc tggaaatcgg catcttctgc
      121 accatccaca ctttacgcct tcactttgaa gaacatttac ccctcgttgt ggccggacgt
      181 cgcctgggcg taccaaaatc aacagtttgt agtatgtttg tgcgcttccg caaagctggc
      241 ctttcatggc ctctgcccgc aggtatgtcg gagcgggaac ttgacgcccg tctttacggg
      301 agtgcctcca cagtacctgt cgtacttact gaaagtaccg ttatgcccga agtccccggg
      361 gtaaaaaagc gtccccggcg ccccaacttc ccttatgagt tcaaaatcgc cttagtggag
      421 cagtcactgc agcccggagc ctgtgtggcg cagattgccc gtgaaaatgg aatcaacgat
      481 aacctgctct tcaactggcg ccatcaatac cggaaaggtg gcctgctgcc ttccggaaaa
      541 aatatgccgg cactgcttc
//
LOCUS       DADLSE010000410          554 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLSE010000410 DADLSE010000000
VERSION     DADLSE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.414"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>554
                     /locus_tag="JN692_004463"
     CDS             <1..>554
                     /locus_tag="JN692_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7266514.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEM"
BASE COUNT          125 a          134 c          162 g          133 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcttggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgtt
//
LOCUS       DADLSE010000411          553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLSE010000411 DADLSE010000000
VERSION     DADLSE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.415"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>553)
                     /gene="ospC4"
                     /locus_tag="JN692_004464"
     CDS             complement(<1..>553)
                     /gene="ospC4"
                     /locus_tag="JN692_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HAY7266515.1"
                     /translation="SGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIIN
                     SNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEIS
                     NHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDN
                     FKEVVDEVSRYVHGNQGKTDVPIF"
BASE COUNT          187 a           80 c          102 g          184 t
ORIGIN      
        1 aaatattgga acatcagttt tcccctgatt accatgcaca tacctgctaa cttcatccac
       61 aacctcttta aagttatcta agaaaaatga ttggtgctca tggtaaaaaa caggtggaat
      121 agcattatca aagtgatcgg ttaatgtcat atatccatat ggagagtcat gatcaatgat
      181 atacgcattg gcgccaaaat ctacagtatg atgtgtttta tttagtggga gtttttcttg
      241 atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg ttagagattt ctttaatatc
      301 ctcagagtat gtattcttat gtgtttgtat attttcagac tgaattttat caaaggattt
      361 aatattaaga atattatttg acagattaga gttactttga tgtctgaagt taaaattaga
      421 attaataata atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat
      481 tatctcagag atatagtgcc tggacacctt atcgagatgc tcaagtgacc gaaatgatga
      541 ccgtgctcca gat
//
LOCUS       DADLSE010000412          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLSE010000412 DADLSE010000000
VERSION     DADLSE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.416"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..207
                     /locus_tag="JN692_004465"
     CDS             <1..207
                     /locus_tag="JN692_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7266516.1"
                     /translation="VYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHM
                     DLYDGKAYVAEAISVLAPFFEETL"
     gene            207..>549
                     /locus_tag="JN692_004466"
     CDS             207..>549
                     /locus_tag="JN692_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7266517.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          144 a          113 c          148 g          144 t
ORIGIN      
        1 gtgtacctga ctcagccgat gcagattgtg gcgggcagcc aggcagggag caaatggatg
       61 agtgacgacc tgtacgatcg ggcatcaagc caggataaac gctatcacat tgttgaaggc
      121 gcaaaccata tggatcttta tgacggcaaa gcctatgtgg ctgaagcaat ttcagtatta
      181 gctccgttct ttgaggaaac tctgtaatga aacatattca aatccgcaat agtgatatgg
      241 cctggcatat tgctgccaat attcagtttc caccaaattt tgatgagtca aaacagtatc
      301 cggcgattat cagcgtccat ccttttggca gttgtaagga gcagacgtct ggcaatatct
      361 atggcaaggc attggctgaa aaaggctatg tcgttctcgc ttatgacgcc agtttccagg
      421 gagaatctgg tggggagcct cgttggatag aagatccgac acagcgcgta gaggatatta
      481 gccgtgttat cgattatgca gtaactctgc catatgttga tgctgaacgc attggcgtgc
      541 tgggtgtgt
//
LOCUS       DADLSE010000413          549 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLSE010000413 DADLSE010000000
VERSION     DADLSE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.417"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..463
                     /locus_tag="JN692_004467"
     CDS             <1..463
                     /locus_tag="JN692_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7266518.1"
                     /translation="LGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLIDH
                     QAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQ
                     VERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          142 a          106 c          120 g          181 t
ORIGIN      
        1 tctgggtggg gagatggata aagatatgat cgccattcga atcgggccag atataccaat
       61 ggctattgtt ggctcaccgg attatttttc tcgtcgaagt gctccaacgt cagtgtcaca
      121 attaatagat catcaggcaa ttaatttgta tcttcccaca tcgggtacag caaatcgctg
      181 gagattaata cgcggtggac gtgaagttcg tgttcgcatg gaaggtcagc ttttactgaa
      241 tacgatagac ctgatcattg atgctgcaat tgatgggcat ggattggcgt atctacctta
      301 tgatcaggtt gagcgggcta ttaaagaaaa aaaactgata cgtgttttgg ataaattcac
      361 tccagattta cccggttatc acctgtacta tccacaccgt cgacatgctg gctcggcatt
      421 ctcattattt atagataggc tgaagtataa aggtgctgtt tagcactact tgctgataca
      481 ttaatttaat tcttctctta acgtattctc agttcctttc aacgttttgg ttgtttttta
      541 ttcttcgta
//
LOCUS       DADLSE010000414          548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLSE010000414 DADLSE010000000
VERSION     DADLSE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.418"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>548
                     /locus_tag="JN692_004468"
     CDS             <1..>548
                     /locus_tag="JN692_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7266519.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLR"
BASE COUNT          178 a          117 c          120 g          133 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaatttattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaacg attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagac
//
LOCUS       DADLSE010000415          546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLSE010000415 DADLSE010000000
VERSION     DADLSE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.419"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(54..242)
                     /locus_tag="JN692_004469"
     CDS             complement(54..242)
                     /locus_tag="JN692_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266520.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            358..546
                     /locus_tag="JN692_004470"
                     /pseudo
     CDS             358..546
                     /locus_tag="JN692_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          138 a          131 c          162 g          115 t
ORIGIN      
        1 gtgaacagac gtttcagcgg gcggggagta aaagcggaca acatgaggac ggattaaccc
       61 ccggtatcct gtagccgggt aagggctgct gtgtcgccac ggcgctccag gatggaggcc
      121 gcgaccatcg caggtggtgg gtccatgggt ttgagcatct tcatccagtc ccattcacgt
      181 tgtgagagct tctcagcacc aacagagagc agaacggtat ctatactttt gcgggtcaac
      241 ataagaggat gagtccttaa taaaacaggt cctcagagca tacatattct gcggagcgtg
      301 cggcaacaac aggacgaaca ggggcggttt tcaatttgca gccgtcaggc tgccgtggtt
      361 cagcgcctga tgggaatcct ctcacttaag gcagcgatta aggtcaagcg ataccgctct
      421 tacagaggag aggtagggca aaccgccccc aatgttctcc aaagagattt caaggctacg
      481 cggccaaacg agaagtgggt taccgatgtt actgaatttg cagtcaatgg gcgcaagctg
      541 tatttg
//
LOCUS       DADLSE010000416          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLSE010000416 DADLSE010000000
VERSION     DADLSE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.420"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="JN692_004471"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="JN692_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HAY7266521.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="JN692_004472"
     CDS             103..375
                     /locus_tag="JN692_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7266522.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT          173 a           95 c          146 g          128 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggg
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 at
//
LOCUS       DADLSE010000417          542 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLSE010000417 DADLSE010000000
VERSION     DADLSE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.421"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(253..>542)
                     /locus_tag="JN692_004473"
     CDS             complement(253..>542)
                     /locus_tag="JN692_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7266523.1"
                     /translation="IGRNDPRKPISKESINQVIELLGYKGRLTGHGFRHTMSTILHEQ
                     GFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSRES"
BASE COUNT          170 a           94 c          100 g          178 t
ORIGIN      
        1 ttataatgat gtcagatgat tgaaatgcta aattatatct gaatcaactt ttcctttgct
       61 aaaggtatcg aaataactgc aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt
      121 tgtttttaat tatgtttttg taataagttt gaagacgcaa aaaaataata taataacaaa
      181 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaac atcagggaaa
      241 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
      301 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
      361 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
      421 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
      481 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
      541 aa
//
LOCUS       DADLSE010000418          518 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLSE010000418 DADLSE010000000
VERSION     DADLSE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.422"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..442
                     /locus_tag="JN692_004474"
     CDS             <1..442
                     /locus_tag="JN692_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7266524.1"
                     /translation="GKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTT
                     LQRGVMAPRLLIIDEIGYLPFSQKEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFA
                     GDAALTSAMLGRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          148 a          126 c          129 g          115 t
ORIGIN      
        1 ggggaaaacc catctggcaa tagcgatggg ctatgaagca gtccgtgcag gtatcaaagt
       61 tcgcttcaca acagcagcag atctgttact tcagttatct acggcacaac gtcagggccg
      121 ttataaaacg acgcttcagc gtggagtaat ggccccccgc ctgctcatca ttgatgaaat
      181 aggctatctg ccgttcagtc agaaagaagc aaaactgttc ttccaggtca tcgctaaacg
      241 ttacgaaaag agcgcaatga tcctgacatc caatctaccg ttcgggcagt gggatcaaac
      301 gttcgccggt gatgcagcac tgacctcagc gatgctgggc cgtatcttac accactcaca
      361 tgtcgttcaa atcaaaggag aaagctatcg actcagacag aaacgaaagg ccggggttat
      421 agcagaagct aatcctgagt aaaacggtgg atcaatattg ggccgttggt ggagatataa
      481 gtggatcact tttcatccgt cgttgacaca acactggc
//
LOCUS       DADLSE010000419          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLSE010000419 DADLSE010000000
VERSION     DADLSE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.423"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..453
                     /locus_tag="JN692_004475"
     CDS             <1..453
                     /locus_tag="JN692_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266525.1"
                     /translation="IQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGWH
                     FFSQRNTDNSITFAVNGQVIPLNYGNFDARYKHRTEGVQDVRYGYEMYYSPGSNTVSW
                     RFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
BASE COUNT          149 a          110 c          128 g          125 t
ORIGIN      
        1 attcaggccg gaactgccag aaaattcacg ttatccagcg ccaacaactc cacgaaaaat
       61 gcagcgtttt atttgtgggg taatccatcc aggcctgttg ttgcagagct tggtgatgat
      121 tcaggctggc attttttcag ccagaggaat acagataaca gcatcacgtt cgccgtaaac
      181 ggacaggtaa ttccgttaaa ttacggaaac ttcgatgccc gctataaaca tcgaactgag
      241 ggggtacagg atgtgcggta tggttatgaa atgtattaca gccccggcag taacaccgtt
      301 tcgtggagat tttgcgcacc ttcgggacac gggctgtcag ggatggcgat atcggatacc
      361 ggccgtaact cagcggataa cgttgacggt gtgtattacc gaccactgca aaaactgatt
      421 aatggcacct ggtataacgt agcgagtatt taacaatgtt gcatttaaaa aatattactg
      481 cgggtaatcc gaaaacagca gaacaatatc ag
//
LOCUS       DADLSE010000420          510 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLSE010000420 DADLSE010000000
VERSION     DADLSE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.424"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..489
                     /locus_tag="JN692_004476"
     CDS             <1..489
                     /locus_tag="JN692_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7266526.1"
                     /translation="KKMAAMFHQQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSV
                     RQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKH
                     LLTIEIRPPMDACIASADNKMIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADP
                     YP"
BASE COUNT          162 a          104 c          112 g          132 t
ORIGIN      
        1 aaaaaaatgg ccgccatgtt tcatcagcaa cgaaatccag tgattgatta tgtctggaat
       61 tcagtacggc gtaaattcgg ggggcgctta catgcccggg aggatgggat aaaaccattt
      121 attcagtcag tacgccaggg atactgggga tattacctcc cagatcagga tcatggtcct
      181 gaatacagtg aatttgctga tttttttgcg acctacaaag cgacattacc aatcattgga
      241 cgtctgatga acatcagtca ggctatgatt ataccacttt tcccggttta tgatgaaaaa
      301 aaacatctcc tgactattga aattcggcca ccaatggatg cttgcattgc cagcgcggac
      361 aataaaatga ttgcccgaca aatgaacaaa acagtggaaa ttttggtggg gccacatccg
      421 gaacagtatg tctgggtttt aaaattgtta aaaacacgca aatcaaacga agcggacccg
      481 tacccttgaa attataaagt acaagtattt
//
LOCUS       DADLSE010000421          507 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLSE010000421 DADLSE010000000
VERSION     DADLSE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.425"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(34..171)
                     /locus_tag="JN692_004477"
                     /pseudo
     CDS             complement(34..171)
                     /locus_tag="JN692_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            232..425
                     /locus_tag="JN692_004478"
                     /pseudo
     CDS             232..425
                     /locus_tag="JN692_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          138 a          130 c          110 g          129 t
ORIGIN      
        1 tccgtaaagt ccctgcaatt tctgaaataa tcaacgtaca gccagggctt cgttgttaat
       61 cgaaccataa taacgaactt cgccaagccg ttcaggtgca gcaatagcaa ctgccaacgt
      121 ttctttatgt acatccagcc tgatgtaagc tgcaatattt tccgaatgca taatgacctc
      181 ctctggaata tctcaccaat gctatagtga gtatataaag gtcataccat ctacatgttc
      241 ttcagcaacg atcacggtgg agagcgtggt gcgttactgt acggtctgat cggcacctgc
      301 cgtttgaacg gtatcgatcc ggaagcgtat ctgcgccata tcctgagtgc actgcctgcc
      361 ggatatggcc ttccaaccgt gttgacgaac tgctgccatg gaacgtagtt ctcaccaata
      421 aataagcgtc aatactgtgc tccgttaacg cttaccactg gcctatacgg agcgactaaa
      481 agaagcaaaa ctgctggcat caacagg
//
LOCUS       DADLSE010000422          483 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLSE010000422 DADLSE010000000
VERSION     DADLSE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.426"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(69..>483)
                     /locus_tag="JN692_004479"
     CDS             complement(69..>483)
                     /locus_tag="JN692_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7266527.1"
                     /translation="FDCVARPWLTEAGSRSARGIWEIEFNHKLLRYIYGLTNQFTTYS
                     LRDCGSLRNPRTIRLYESLAQFKSSGLWVTTHAWLNDRFLLPESQQKNLAELKRSFLD
                     PALKQINEKTPLLAKYSIDDSGKFLFSIIDKQNPV"
BASE COUNT          136 a           97 c          117 g          133 t
ORIGIN      
        1 gtgtggaaat tcgcccatct tcatgacaaa aatttgtcaa atgacaggct catgtgtgct
       61 gatttatgtc agacgggatt ttgcttatca attattgaga acagaaattt tcctgaatca
      121 tcaatactat acttagcaag taaaggtgtt ttctcattta tctgcttgag tgcaggatca
      181 aggaaagatc gtttcaactc tgccaagttc ttctgttggg attccggcaa aaggaaacgg
      241 tcatttaacc aagcatgagt agtaacccat aagcctgaag atttgaattg agcaagactt
      301 tcataaaggc ggatcgtccg tggatttcga agactgccac aatcgcggag cgagtaggtg
      361 gtgaactggt tcgtcaggcc gtaaatgtac cgcaggagtt tatggttaaa ttcgatttcc
      421 cagataccac gagctgatcg gctgccagcc tctgttagcc aggggcgcgc gacgcagtca
      481 aaa
//
LOCUS       DADLSE010000423          469 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLSE010000423 DADLSE010000000
VERSION     DADLSE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.427"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            82..>469
                     /gene="yiaM"
                     /locus_tag="JN692_004480"
     CDS             82..>469
                     /gene="yiaM"
                     /locus_tag="JN692_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HAY7266528.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTS"
BASE COUNT          118 a          103 c           95 g          153 t
ORIGIN      
        1 ttattgctct aactatatta ataatttagg ttgctgcaca tgcaacttga attatgacag
       61 acatagcctc aaggaatagc tatgaaaaaa atacttgaag caatactggc gattaatctc
      121 gccgtacttt cctgtattgt gtttataaac attattttaa gatatggttt tcagacaagc
      181 attttatctg tagatgaatt gtcacgttat ttatttgtct ggttaacatt tattggcgcg
      241 attgtggctt ttatggataa cgcccacgtt caggtcacct ttctggtgga gaaactctcc
      301 cccgcatggc aacgacgagt cgctctggtt acccattcct taatcttatt tatttgtggc
      361 gcactggcct ggggcgcaac gctaaaaaca attcaggact ggagcgatta ttcaccgatc
      421 ctcggtttgc ccatcggcct gatgtatgcc gcttgtctgc ccaccagcc
//
LOCUS       DADLSE010000424          454 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLSE010000424 DADLSE010000000
VERSION     DADLSE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.428"
                     /strain="HUE07"
                     /db_xref="taxon:623"
BASE COUNT          136 a           98 c           68 g          152 t
ORIGIN      
        1 tctgtaatcc tctttgcttc ctgagtaata acttcctgag tgaatattta acctgagctt
       61 gatcctacac atacttatta tgaatgataa aattcattca attaataaca catatattaa
      121 ttgccgttaa aactaaaaac agcatcaata atcaacgcga tataataaac ctgccttaca
      181 tatcaactgc gccagaggta ggattgaaaa cgctctcctg attttccaat tcattttctg
      241 gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt cagggagagg
      301 gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac cggagcattt
      361 tgcatggaat tttcgttttc acccaaacgt cttgttgttg ctgtcgccgc cgctcttcct
      421 ctcatggcca gcgcagcaga taccccgtca actg
//
LOCUS       DADLSE010000425          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLSE010000425 DADLSE010000000
VERSION     DADLSE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.429"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..144
                     /locus_tag="JN692_004481"
     CDS             <1..144
                     /locus_tag="JN692_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7266529.1"
                     /translation="GDTAVSKGLDVTGQSRLSGGSNIGGIDFGNHVHGGVESGGSTTQ
                     GPR"
     gene            157..>443
                     /locus_tag="JN692_004482"
     CDS             157..>443
                     /locus_tag="JN692_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266530.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTAD"
BASE COUNT           97 a          124 c          128 g           94 t
ORIGIN      
        1 ggcgataccg ccgtcagtaa ggggcttgac gttaccgggc agtccaggct ttccggcggt
       61 tcgaacatcg ggggtattga ttttggaaac cacgttcacg gcggcgttga gtccggcggc
      121 tcaaccacgc agggaccccg gtaaacagga gaaaacatgc agtcacgatc acttcttctt
      181 gacaccggga catgggatat cctgcttgat gataccggaa atcttgccat tactgataat
      241 ccccatgcgg tagctcagga tgtggcgtgt gcgtgcagta cttttctggg ggagtgctgg
      301 tacgactcaa cggctggcat accttactgg ccacgcatcc tcggacactg gcccggcaca
      361 caactggtga atgccactct gcaacaggaa gcacttaaac tgccgaccgt gagcgccgct
      421 gtctgccagg tcacagctga tca
//
LOCUS       DADLSE010000426          443 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLSE010000426 DADLSE010000000
VERSION     DADLSE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.430"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>443)
                     /locus_tag="JN692_004483"
     CDS             complement(<1..>443)
                     /locus_tag="JN692_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7266531.1"
                     /translation="QPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRL
                     PRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAG
                     QVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYEFICT"
BASE COUNT          103 a          124 c          127 g           89 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg tgccaggaag
      361 ccaccgctgg cagcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc ctggaattta
      421 tccagcgcat cgtgcagcgg ttg
//
LOCUS       DADLSE010000427          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLSE010000427 DADLSE010000000
VERSION     DADLSE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.431"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            161..>442
                     /locus_tag="JN692_004484"
     CDS             161..>442
                     /locus_tag="JN692_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7266532.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQS"
BASE COUNT          136 a           81 c           89 g          136 t
ORIGIN      
        1 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
       61 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
      121 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      181 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      361 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      421 gtttgccaca gcattgcaat cc
//
LOCUS       DADLSE010000428          439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLSE010000428 DADLSE010000000
VERSION     DADLSE010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.432"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..128
                     /locus_tag="JN692_004485"
     CDS             <1..128
                     /locus_tag="JN692_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7266533.1"
                     /translation="GRESAPDASSVIHATGGDRVATSQTDRTAWHPDITRDKTRE"
     gene            125..>439
                     /locus_tag="JN692_004486"
     CDS             125..>439
                     /locus_tag="JN692_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7266534.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSE"
BASE COUNT          134 a          107 c          118 g           80 t
ORIGIN      
        1 cgggaaggga aagtgcacct gacgccagct cagttatcca tgctactgga ggggatcgcg
       61 tggcaacatc ccaaacggac agaacggcct ggcatccgga tataacccgt gataaaacaa
      121 gggaatgaac aatgaactcc ccgatgatat tgagctgctt aaagccatgt tgcgtaagca
      181 acagagtcgg cttcgacagt atgcctgtca ggtcgcgggc tatgagcagg aaattgaacg
      241 gctgaaagcg caactcgaca ggttgcgtcg tatgttgttc ggccagagtt cagagaaaaa
      301 gcgtcataag cttgaaaatc agatccgaca ggcagaaaaa cgactgtcgg aactggaaaa
      361 ccggctgaac acagccagaa atcttctgga agatgcatcg tcagtcacag attcacctga
      421 caccagtccc ccgtcagaa
//
LOCUS       DADLSE010000429          436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLSE010000429 DADLSE010000000
VERSION     DADLSE010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.433"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(322..>436)
                     /locus_tag="JN692_004487"
     CDS             complement(322..>436)
                     /locus_tag="JN692_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7266535.1"
                     /translation="YLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          155 a           66 c           71 g          144 t
ORIGIN      
        1 gatgtgacaa catctatttt cattgatttg attcaatata acaatagatt acagaggatt
       61 aacttccagt tacttaaaaa ataaagaaaa aagtgtggaa acaggagaat ttaagtgatg
      121 tgtaaactta tgaatgattt tgtttattta ttttgaagaa cataaaccat tagattttgt
      181 atgggtatgg ttaacttaat gtttattatg ataattaaga atcacactta caataacatc
      241 aattaataaa tggttaatca tttttgatat atcgtaagaa taatgtagtt tttaacacca
      301 tccctggtat ctcaactatc tctataaaac agcatgacgc tgtcgtcctc tggctctatc
      361 ccagatgccg taaaaacgcc ctgcattgct ggcggtatac cagacagtat gacgaatatt
      421 gcgatgccca agataa
//
LOCUS       DADLSE010000430          433 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLSE010000430 DADLSE010000000
VERSION     DADLSE010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.434"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>433
                     /gene="ipaH9.8"
                     /locus_tag="JN692_004488"
     CDS             <1..>433
                     /gene="ipaH9.8"
                     /locus_tag="JN692_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7266536.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTAN"
BASE COUNT           96 a          121 c          113 g          103 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gtcagaactc tccattttgt ggatgagata gaagtctacc tggccttcca gaccatgctc
      361 gcagagaaac ttcagctctc cactgccgtg aaggaaatgc gtttctatgg cgtgtcggga
      421 gtgacagcaa atg
//
LOCUS       DADLSE010000431          424 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLSE010000431 DADLSE010000000
VERSION     DADLSE010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.435"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..205)
                     /locus_tag="JN692_004489"
     CDS             complement(<1..205)
                     /locus_tag="JN692_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266537.1"
                     /translation="MRLWADGRLIFEGDITDAYADYNQAPDIPLILTGQIHFNLRNQP
                     AADFSAKGDVAVADIIRALASTVG"
BASE COUNT          103 a          127 c           94 g          100 t
ORIGIN      
        1 gcccgacagt tgacgccagc gcacggataa tatctgcaac cgcaacatcg ccctttgcgc
       61 tgaaatctgc cgccggctga ttacgcagat taaaatgaat ctgtcctgtc agaatgagcg
      121 gaatatccgg cgcctggttg tagtcggcgt atgcatcagt aatatcacct tcaaaaataa
      181 gccgcccgtc ggcccacagc cgcattttat ttgcggtact ctgctcctgt cgccacacac
      241 cgcgccagct taaattcgtc atatgcgccg gtgacattcc ccagatatac agcgtgattt
      301 gtgtaccagc cacccctcca tatacggcaa tactggcaaa acagcttgcg tcggtaatgg
      361 tcagcatatt gccctggcca ttaccaaacg ttcgtccgtc tgaaagagtg aactccaccg
      421 ttat
//
LOCUS       DADLSE010000432          411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLSE010000432 DADLSE010000000
VERSION     DADLSE010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.436"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>411)
                     /locus_tag="JN692_004490"
     CDS             complement(<1..>411)
                     /locus_tag="JN692_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAY7266538.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEA"
BASE COUNT          116 a          101 c           72 g          122 t
ORIGIN      
        1 gcttcagcta gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt
       61 gataatgctg tcagtgctcg cttaatctct tcttcacttc gccccatcag acgagaatag
      121 ccaatcatat ttctaaaaag ttcttcagaa ccttgaatgc ctagagactc tctagctgaa
      181 gcaccgaaac cagcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct
      241 aatgactttg tgaaatcttt tacttgattt gcatctttag aaaatacgaa atcaattgaa
      301 cgttcagcac ttgcacgttg aaggccaact tccattactc gctgataagc ggatagtaca
      361 gcctgaaagc tgataagtac acctgctgtt ttaactgctg ctgaacttaa a
//
LOCUS       DADLSE010000433          405 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLSE010000433 DADLSE010000000
VERSION     DADLSE010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.437"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..392)
                     /locus_tag="JN692_004491"
     CDS             complement(<1..392)
                     /locus_tag="JN692_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7266539.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKRCLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSL"
BASE COUNT          107 a           76 c          115 g          107 t
ORIGIN      
        1 agtgaaacat caagagcgac caggtgaggc ggaagagccg ggagcctgct cagtctgtta
       61 tcgctggctt taagtacagt taaggacgga gggagttctg gcagagagcg tagctcattg
      121 ctagagatgt taagttcttg tatgtgcggg ggcaggtatg ggagagagcg taatctgagt
      181 aaacttaaat tgagggctgg ctcttgaaaa gccagacatc ttttcagtaa tcgaactgcc
      241 tgtgttcggt cttctgttgc agcaccttcc ttggcccagt tatcccagat gcggtgataa
      301 tccgtgatat tttgctcttc tacggataaa cgggatatac atcctaaacc acaaaaggag
      361 cggaaaaaag aaggattgtg ggcaggtttc atttattatt gatcc
//
LOCUS       DADLSE010000434          404 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLSE010000434 DADLSE010000000
VERSION     DADLSE010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.438"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>404)
                     /locus_tag="JN692_004492"
     CDS             complement(<1..>404)
                     /locus_tag="JN692_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266540.1"
                     /translation="PQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKL
                     LTDAVKMRGLIAPASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLN
                     AQKLRDTQHMMQQILLSLVHALYEGNPQPVFA"
BASE COUNT           93 a           91 c          112 g          108 t
ORIGIN      
        1 ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaggctca gcagtatttg
       61 ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg
      121 gcgcgtggcc cagtatgcat tgatttgcaa ctccagcata caaaccagat tgcgattaat
      181 ggtctggata ccttcatata tcgatttggg aatacgggtt tctttgctgg cgggcgcaat
      241 cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg
      301 gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact
      361 tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggcc
//
LOCUS       DADLSE010000435          391 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLSE010000435 DADLSE010000000
VERSION     DADLSE010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.439"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..278
                     /locus_tag="JN692_004493"
     CDS             <1..278
                     /locus_tag="JN692_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7266541.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          100 a           91 c          104 g           96 t
ORIGIN      
        1 gcctgaacgt gtactgtcgt aatggccagg tggaaatcga caacaacatc ggtgaaaacg
       61 cgctgcgatc ggtggcggtt ggaagaaaaa attatctctt cttcggttct gacaacggtg
      121 gagaaagcgc ggcgattatc tacagtctgc tggtcacctg caaactgaac ggagtggagc
      181 cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg aaccgagtgc
      241 atgaactgct gccctggaac ttctcagctg taaaataatc tttccgctac gtacttctct
      301 gggcgcttac cactcaacgg catcgtcaaa attatcaaag cgcagaacat agatttatca
      361 tgtgttttat ggatggcttt ctccatcagg c
//
LOCUS       DADLSE010000436          387 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLSE010000436 DADLSE010000000
VERSION     DADLSE010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.440"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..362)
                     /locus_tag="JN692_004494"
     CDS             complement(<1..362)
                     /locus_tag="JN692_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7266542.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYP"
BASE COUNT          113 a           80 c           68 g          126 t
ORIGIN      
        1 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
       61 ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
      121 agcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct
      181 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc
      241 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
      301 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
      361 attattactc cggaaaatgg aagcgac
//
LOCUS       DADLSE010000437          386 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLSE010000437 DADLSE010000000
VERSION     DADLSE010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.441"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            1..386
                     /locus_tag="JN692_004495"
                     /pseudo
     CDS             1..386
                     /locus_tag="JN692_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           92 a          101 c          119 g           74 t
ORIGIN      
        1 tggctgtacg ccggagactc tgcgtgtctg ggttcgccag catgagcggg ataccgggga
       61 cggtgatgga gggctcacca ccgctgaacg tcagcgtctg aaagagctgg agcgtgaaaa
      121 tcgtgaactg cgccgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc
      181 ggagttcgac cgcctctgga aaaaatgatg ccactgctgg ataagctgcg tgagcagtac
      241 ggggtcggac cgctatgcag cgaactgcat attgccccgt caacgtatta ccactgtcag
      301 caacagcgac atcatccgga taaacgcagt gcccgtgcgc agcgcgatga ctggctgaag
      361 aaagagatac agcgcgtata cgatga
//
LOCUS       DADLSE010000438          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLSE010000438 DADLSE010000000
VERSION     DADLSE010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.442"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>375
                     /locus_tag="JN692_004496"
     CDS             <1..>375
                     /locus_tag="JN692_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7266543.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLSE010000439          373 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLSE010000439 DADLSE010000000
VERSION     DADLSE010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.443"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>373
                     /locus_tag="JN692_004497"
     CDS             <1..>373
                     /locus_tag="JN692_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266544.1"
                     /translation="CHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRL
                     TLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMT
                     LQGASPGRIPELMRDLASMGQL"
BASE COUNT          103 a           73 c          116 g           81 t
ORIGIN      
        1 tgccatctgc tgacgtcgat gacggacgcc atgcgcttcc ccggaggaga aatggcggat
       61 ctgtacagtc atcgctggga aatcgaactg ggatacaggg agataaaaca gacgatgcaa
      121 ctgagcaggc tgacgctgag aagtaaaaag ccggagcttg tggagcaaga gctgtggggt
      181 gtcttactgg cttataatct ggtgagatat cagatgatta aaatggcgga acatctgaaa
      241 ggttactggc cgaatcaact gagtttctca gaatcatgcg gaatggtgat gagaatgctg
      301 atgacattgc agggcgcttc accgggacgt ataccggagc tgatgcgcga tcttgcaagt
      361 atgggacaac ttg
//
LOCUS       DADLSE010000440          364 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLSE010000440 DADLSE010000000
VERSION     DADLSE010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.444"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>364
                     /locus_tag="JN692_004498"
     CDS             <1..>364
                     /locus_tag="JN692_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266545.1"
                     /translation="RQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAE
                     QLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHL
                     VKLKTSPQARKKWPGLGN"
BASE COUNT          111 a           91 c          102 g           60 t
ORIGIN      
        1 cccgccagac acatgccggg aacccggcgc tctacccgca ggtcaaaatg gtctgccaga
       61 tggaactgac cagccatctg ctgacggctg cagccttcgg cacgatgaag aacagcgaaa
      121 atgagcttgc tgagcaactt atagaacaaa ccggcgataa cactctgacg ttaatggata
      181 aaggttatta ctcactggga ctgttaaatg cctggagcct ggcgggagaa caccgccact
      241 ggatgatacc tctcagaaag ggagcgcaat atgaagagct cagaaaactg ggtaaaggcg
      301 atcatctggt gaagctgaaa accagcccgc aggcacgaaa aaagtggccg ggactgggaa
      361 atga
//
LOCUS       DADLSE010000441          353 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLSE010000441 DADLSE010000000
VERSION     DADLSE010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.445"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..347)
                     /locus_tag="JN692_004499"
     CDS             complement(<1..347)
                     /locus_tag="JN692_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266546.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEW"
BASE COUNT           69 a          102 c           86 g           96 t
ORIGIN      
        1 ttccattcaa acgtgaacgg cttcgcctcc tgttctgcgc gccatgcttc aatttcctga
       61 cgcctggcct ctctggccgc ttccagcatt tctggtgtca cagtgaatgg ggctatctca
      121 ccccatttgc cgctttgcag ttcctgccag atttgctgac ccgtcggagc gacatcatca
      181 gcggtggctg tgtaggggac tgcctggtcc ctgtcgtcaa aaaaaacgtc acagtctact
      241 gcgccacttt cggtataacg gggattaatg atttttttaa tttccacggt gcattcctca
      301 cgatgtgcga ataaaaagcc cgggcattgc gccagagaca tgtgcatccg gca
//
LOCUS       DADLSE010000442          347 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLSE010000442 DADLSE010000000
VERSION     DADLSE010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.446"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>347
                     /locus_tag="JN692_004500"
     CDS             <1..>347
                     /locus_tag="JN692_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7266547.1"
                     /translation="LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRL
                     ALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAA
                     HLAAYAGLAPVTR"
BASE COUNT           63 a          120 c           95 g           69 t
ORIGIN      
        1 agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca
       61 tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag
      121 tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc
      181 ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg
      241 gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct
      301 ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcgg
//
LOCUS       DADLSE010000443          332 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLSE010000443 DADLSE010000000
VERSION     DADLSE010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.447"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            161..>332
                     /locus_tag="JN692_004501"
     CDS             161..>332
                     /locus_tag="JN692_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7266548.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLS"
BASE COUNT           97 a           56 c           67 g          112 t
ORIGIN      
        1 cgaaaacgat acgctttgtt ggattatatc catgaaactg ctatccgctc tttggatgca
       61 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      121 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      181 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt
      241 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      301 caaaataggg cgttgggtgg gcagattgag ca
//
LOCUS       DADLSE010000444          314 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLSE010000444 DADLSE010000000
VERSION     DADLSE010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.448"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            24..100
                     /locus_tag="JN692_004502"
     tRNA            24..100
                     /locus_tag="JN692_004502"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="JN692_004503"
     tRNA            143..218
                     /locus_tag="JN692_004503"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           87 a           67 c           78 g           82 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aatt
//
LOCUS       DADLSE010000445          311 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLSE010000445 DADLSE010000000
VERSION     DADLSE010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.449"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>311)
                     /locus_tag="JN692_004504"
     CDS             complement(<1..>311)
                     /locus_tag="JN692_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7266549.1"
                     /translation="GGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHE
                     GDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDCDYDGEHRDE
                     P"
BASE COUNT           80 a           88 c           71 g           72 t
ORIGIN      
        1 cggttcatcc cgatgctcac cgtcgtaatc gcaatcatat tcgtacccct ccattcgacg
       61 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
      121 aaaattggtg ccgaaggtgt tcatcgattt cgcatagagg aagcgaatgg aatccccctc
      181 atgacgactg tcgtcggcta actgactgcg cgcatgggtc acatccagcg acactgcccc
      241 ccagtttccg agattgcgcc ccagtcctaa taaaaaggcg gtgtaattgg cagataattg
      301 cgtcccgccg t
//
LOCUS       DADLSE010000446          310 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLSE010000446 DADLSE010000000
VERSION     DADLSE010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.450"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..306
                     /locus_tag="JN692_004505"
     CDS             <1..306
                     /locus_tag="JN692_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HAY7266550.1"
                     /translation="KNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENK
                     GLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT           87 a           67 c           72 g           84 t
ORIGIN      
        1 aagaacgggc gcttcggatt tggtcttcgg gccagcaaaa gtcgttcaac aggaagaatt
       61 ctggttctga gtctgctggc aacgttgagc actatcgtaa tgtggttact cggttatcac
      121 gctgaaaata aagggttaca cctgaaatat caggctaaca gcattaaatc ccggcgggtt
      181 atctcatatc tgacgttagc gaagaatgtt cttcgacact cgccacttat tttaagacga
      241 acagtactga gcacagttct aaaccatctg tccagaacct accgaaatat ggtgctggtt
      301 tattagcagt
//
LOCUS       DADLSE010000447          309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLSE010000447 DADLSE010000000
VERSION     DADLSE010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.451"
                     /strain="HUE07"
                     /db_xref="taxon:623"
BASE COUNT          103 a           61 c           47 g           98 t
ORIGIN      
        1 ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac
       61 ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt
      121 tatttctttc ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga
      181 aatccattta aaaatggcta catcccttat acctattcag agctacttat tcaggggtta
      241 ttgagatata cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat
      301 agtcggaat
//
LOCUS       DADLSE010000448          303 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLSE010000448 DADLSE010000000
VERSION     DADLSE010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.452"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(1..237)
                     /locus_tag="JN692_004506"
                     /pseudo
     CDS             complement(1..237)
                     /locus_tag="JN692_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          106 a           42 c           45 g          110 t
ORIGIN      
        1 catctataaa aatgtgcatc atctcagatg agtatattga aaaaaatgat gaaggactgg
       61 agatggttta ggcaaatcct gacttctgaa aaagtcatca aacaaaatta agaactctgt
      121 tttatactcg caaaacactt ttactaactc attttgtctt gttttattta ttattacatc
      181 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ctgtcattat
      241 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
      301 aaa
//
LOCUS       DADLSE010000449          302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLSE010000449 DADLSE010000000
VERSION     DADLSE010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.453"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>302
                     /gene="virK"
                     /locus_tag="JN692_004507"
     CDS             <1..>302
                     /gene="virK"
                     /locus_tag="JN692_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7266551.1"
                     /translation="GAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISECL
                     AVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIA"
BASE COUNT           79 a           61 c           78 g           84 t
ORIGIN      
        1 ggagccccta aaaataccgg acctgatgtt attcggtgcg caacccgggc ctgttatggg
       61 attttcccaa aacggattat ttttgaagca ttttgtgcgt tgatgaaagc ttgcaatatc
      121 tctgagtgtc tggcagtcag tgagcacagt catgttttcc ggcaattgag atactggtat
      181 cagaaacgca aaacctttgt tgcggtctat agtgatttct gggagtctgt agcaggaaaa
      241 acctgtggtg actggtacag attaccaacc caggtgattc gaaagccgct aagcgatata
      301 gc
//
LOCUS       DADLSE010000450          301 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLSE010000450 DADLSE010000000
VERSION     DADLSE010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.454"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..>301)
                     /locus_tag="JN692_004508"
     rRNA            complement(<1..>301)
                     /locus_tag="JN692_004508"
                     /product="23S ribosomal RNA"
BASE COUNT           66 a          108 c           57 g           70 t
ORIGIN      
        1 ctggaaaacc agcctacacg cttaaaccgg gacaaccgtc gcccggccaa catagccttc
       61 tccgtccccc cttcgcagta acaccaagta caggaatatt aacctgtttc ccatcgacta
      121 cgcctttcgg cctcgcctta ggggtcgact caccctgccc cgattaacgt tggacaggaa
      181 cccttggtct tccggcgagc gggcttttca cccgctttat cgttacttat gtcagcattc
      241 gcacttctga tacctccagc atacctcaca gtacaccttc acaggcttac agaacgctcc
      301 c
//
LOCUS       DADLSE010000451          297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLSE010000451 DADLSE010000000
VERSION     DADLSE010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.455"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            98..>297
                     /locus_tag="JN692_004509"
     CDS             98..>297
                     /locus_tag="JN692_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7266552.1"
                     /translation="MRITTLASVVIPCLGFSASSIAATEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEAL"
BASE COUNT           74 a           61 c           70 g           92 t
ORIGIN      
        1 tcaataatct ttattttttg taaaaggcaa atatattctc attattgttt gtatttgtgt
       61 attgtcttgc cggttaacat gatcggagat tagtaatatg cgaataacca ctctggcttc
      121 cgtagtcatt ccctgtctcg gattttcagc cagcagcata gctgctacag aggatgtgat
      181 gattgtctcg gcatccggct atgagaaaaa gctgactaac gcagccgcca gtgtttctgt
      241 gattagccag gaggaattgc agtccagcca gtaccacgat ctggcggagg ctctgag
//
LOCUS       DADLSE010000452          294 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLSE010000452 DADLSE010000000
VERSION     DADLSE010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.456"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..293)
                     /locus_tag="JN692_004510"
     CDS             complement(<1..293)
                     /locus_tag="JN692_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7266553.1"
                     /translation="MTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENG
                     DIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN"
BASE COUNT           81 a           89 c           60 g           64 t
ORIGIN      
        1 tcattatcac caagaacaat tgaattcccc cccagggcgt tctgaacgcc gatacccagc
       61 gcaccattga cctgcgaacc accgccaaca gacactttat gcgacatgga tatttcaccc
      121 gtccgcaaat taatggtgaa cgggcgcagg ggaccaatat cgccattttc cccctgacct
      181 tcactggtgg gaataaggtg caggcactct tccgaacgac gaaaaatcag gccaaaagct
      241 tcgttgaaaa tcctcagcgc attaacacca cggattttca gctctccggt tatt
//
LOCUS       DADLSE010000453          291 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLSE010000453 DADLSE010000000
VERSION     DADLSE010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.457"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..>291
                     /locus_tag="JN692_004511"
     CDS             <1..>291
                     /locus_tag="JN692_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7266554.1"
                     /translation="KRDLPTAEEEISRVQASIIKWYASEYYNGRQVLDEIQTPSAINS
                     ELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISV"
BASE COUNT           95 a           55 c           60 g           81 t
ORIGIN      
        1 ataagagaga tttaccaact gctgaagagg aaatatctcg cgtgcaggcg tctattatca
       61 aatggtatgc gtcagaatac tataatggca ggcaagttct cgatgagata caaacgcctt
      121 cagcaattaa cagtgagctt tatacaaaaa tgatatacct gaccaggaac tggtcattgt
      181 atccgaacgg tgatggctgt gtaactataa gctcaccaga aataaaaaat aaataccctg
      241 ctgccatttg cctggctctg ggattttttc taagcattgt aatttctgta a
//
LOCUS       DADLSE010000454          284 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLSE010000454 DADLSE010000000
VERSION     DADLSE010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.458"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..258
                     /locus_tag="JN692_004512"
     CDS             <1..258
                     /locus_tag="JN692_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HAY7266555.1"
                     /translation="TVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTSTLLRKIN
                     QGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT           75 a           70 c           77 g           62 t
ORIGIN      
        1 actgtcgcca gacaaattaa cccgtacatc aaagtcgata taccggaaac aacgcgcggc
       61 gctctttact cgttcgttta caacgtgggc gctggcaatt tcagaacatc gacgcttctt
      121 cgcaaaataa accagggcga tatcaaaggc gcatgtgacc agctacgtcg ctggacatat
      181 gctggcggta agcaatggaa agggctgatg acccgtcgtg agattgagcg tgaagtctgt
      241 ttgtgggggc agcaatgagc agagtcaccg cgattatctc cgct
//
LOCUS       DADLSE010000455          281 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLSE010000455 DADLSE010000000
VERSION     DADLSE010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.459"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(<1..259)
                     /locus_tag="JN692_004513"
     CDS             complement(<1..259)
                     /locus_tag="JN692_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7266556.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML"
BASE COUNT           82 a           65 c           77 g           57 t
ORIGIN      
        1 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
       61 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
      121 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
      181 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
      241 taaaaaaatc cggaaacaag tgagcgtttc cggattctta c
//
LOCUS       DADLSE010000456          278 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLSE010000456 DADLSE010000000
VERSION     DADLSE010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.denovo.460"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            36..>278
                     /locus_tag="JN692_004514"
     CDS             36..>278
                     /locus_tag="JN692_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7266557.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKR"
BASE COUNT           82 a           63 c           78 g           55 t
ORIGIN      
        1 tcgaacaatg gcgacaacaa tttcccattg aagcgatgtg tcaggtattt ggtgtatcca
       61 ggagcggtta ttacaactgg gtacagcatg aaccctcaga cagaaaacaa agtgatgagc
      121 ggctaaaact ggagattaag gtggcacata tccgcactcg cgaaacatat ggaacccggc
      181 ggctccagac ggagctggca gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc
      241 ttcgtaagga gctaaggcta cgctgtaagc agaaacgc
//
LOCUS       DADLSE010000457         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.guided.136, whole genome shotgun sequence.
ACCESSION   DADLSE010000457 DADLSE010000000
VERSION     DADLSE010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.guided.136"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            35..559
                     /locus_tag="JN692_004515"
                     /pseudo
     CDS             35..559
                     /locus_tag="JN692_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            675..1235
                     /locus_tag="JN692_004516"
                     /pseudo
     CDS             675..1235
                     /locus_tag="JN692_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN692_004517"
     CDS             complement(1292..2749)
                     /gene="pepD"
                     /locus_tag="JN692_004517"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7266558.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3011..3469
                     /gene="gpt"
                     /locus_tag="JN692_004518"
     CDS             3011..3469
                     /gene="gpt"
                     /locus_tag="JN692_004518"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7266559.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3561..4805
                     /gene="frsA"
                     /locus_tag="JN692_004519"
     CDS             3561..4805
                     /gene="frsA"
                     /locus_tag="JN692_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7266560.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4863..5264
                     /locus_tag="JN692_004520"
     CDS             4863..5264
                     /locus_tag="JN692_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7266561.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5303..6358)
                     /gene="phoE"
                     /locus_tag="JN692_004521"
     CDS             complement(5303..6358)
                     /gene="phoE"
                     /locus_tag="JN692_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7266562.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            6646..7749
                     /gene="proB"
                     /locus_tag="JN692_004522"
     CDS             6646..7749
                     /gene="proB"
                     /locus_tag="JN692_004522"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7266563.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7761..9014
                     /gene="proA"
                     /locus_tag="JN692_004523"
     CDS             7761..9014
                     /gene="proA"
                     /locus_tag="JN692_004523"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7266564.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIVEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9129..9204
                     /locus_tag="JN692_004524"
     tRNA            9129..9204
                     /locus_tag="JN692_004524"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9162..9164,aa:Thr,seq:cgt)
     gene            complement(9219..9917)
                     /locus_tag="JN692_004525"
                     /pseudo
     CDS             complement(9219..9917)
                     /locus_tag="JN692_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         2382 a         2478 c         2605 g         2490 t
ORIGIN      
        1 tccctcaaag tgatctacta ttccgcgcag ctatttaggg cagtctattt tacagcggca
       61 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
      121 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
      181 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
      241 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
      301 acgcggtgat tactcctggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
      361 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
      421 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
      481 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
      541 tctgggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
      601 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
      661 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
      721 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
      781 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
      841 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
      901 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
      961 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
     1021 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
     1081 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
     1141 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
     1201 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
     1261 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
     1321 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
     1381 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
     1441 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
     1501 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
     1561 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
     1621 tcaccacgta gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt
     1681 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
     1741 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
     1801 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
     1861 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
     1921 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
     1981 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
     2041 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
     2101 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
     2161 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
     2221 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
     2281 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
     2341 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
     2401 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
     2461 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
     2521 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa
     2581 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
     2641 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
     2701 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
     2761 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
     2821 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
     2881 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     2941 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3001 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3061 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3121 tggcggtctg gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
     3181 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
     3241 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
     3301 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
     3361 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
     3421 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
     3481 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
     3541 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
     3601 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
     3661 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
     3721 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
     3781 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
     3841 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
     3901 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
     3961 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
     4021 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
     4081 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
     4141 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
     4201 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
     4261 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
     4321 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
     4381 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
     4441 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
     4501 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
     4561 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
     4621 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
     4681 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
     4741 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
     4801 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
     4861 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
     4921 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
     4981 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
     5041 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
     5101 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
     5161 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
     5221 cggcggatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
     5281 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
     5341 cattattaat attaagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
     5401 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
     5461 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
     5521 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
     5581 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
     5641 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
     5701 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
     5761 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
     5821 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
     5881 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
     5941 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
     6001 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
     6061 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
     6121 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
     6181 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
     6241 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
     6301 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
     6361 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
     6421 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaaaatgtg
     6481 gtaatttatt aaatctgtaa taaaagcgta aacaactgcc gctacgcttg ctgatcccgc
     6541 gcaacaaaac gccatgcttt gctcgcagat ggttggcaac cgacgacagt cctgctaaaa
     6601 cgttcgtttg atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag
     6661 acgctggtgg taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt
     6721 gcccatatcg ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt
     6781 gttattgtga cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta cccggaactg
     6841 ccagcgacta tcgcctcgaa acaactgctg gcggcggtag ggcagagtcg actgattcaa
     6901 ctgtgggaac agctgttttc gatttatggc attcacgtcg ggcaaatgct gctgacccgt
     6961 gctgatatgg aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc
     7021 gataacaata tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaag
     7081 gtcggcgata acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg
     7141 ttgctgctga ccgatcaaaa aggtttgtat accgctgatc cgcgcagcaa tccgcaggca
     7201 gaactgatta aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc
     7261 gtttcaggcc tcggaactgg cggcatgagt accaaattgc aggccgctga cgtggcttgc
     7321 cgtgcgggta tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg
     7381 atggaaggca tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt
     7441 aaacgctgga ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact
     7501 gccgccattc tggaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc
     7561 aatttctcgc gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac
     7621 ggtgtcagtc gttacaacag cgatgcatta cgccgtattg ccggacacca ctcgcaagaa
     7681 attgatgcaa tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt
     7741 acccgttaag gagctggccg atgctggaac aaatgggcat tgccgcgaag caagcctcgt
     7801 ataaattagc gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg
     7861 aactggaagc acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc
     7921 gagccaatgg ccttagcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga
     7981 aaggcattgc cgacgatgta cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa
     8041 tcgatggcgg cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg
     8101 ttattggcgt gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc
     8161 tgaaaaccgg taatgcggtg atcctgcgtg gtggtaaaga aacctgtcgc actaacgcgg
     8221 caacggtggc ggtgattcag gacgccctga aatcctgtgg cttaccggcg ggtgccgtgc
     8281 aggcgattga taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca
     8341 tcgacatgct gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt gaacagtcga
     8401 caatcccggt gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgcag
     8461 agatcgttga agcattaaaa gtgatcgtca atgcgaaaac tcagcgtccg agcacatgta
     8521 atacggttga aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa
     8581 gcaaacaaat ggcggaaagc ggcgtgacat tacacgcaga tgcagctgcg ctgacacagt
     8641 tgcaggcagg ccctgcgaag gtggtggcgg ttaaagccga agagtatgac gatgagtttc
     8701 tgtcattaga tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc gcccatattc
     8761 gtgaacacgg cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc
     8821 gttttgttaa cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg
     8881 acggcggcca gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc
     8941 gtggcccaat ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca
     9001 ccattcgtgc gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat
     9061 tgacgcatcg cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc
     9121 ctttcagggc cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag
     9181 gttcgactcc tattatcggc accattaaaa tcaataagtt acacatcatt agtaccttcc
     9241 ttattttttg actgggacaa atttgggacc gatgggttca ggatcgagtc tatttgccgt
     9301 gcgtgttcgg taaggtgatt aggtgcaagg tgagcatatc gacgaaccat ttcgatagac
     9361 tcccagcctc ccatttcctg taacactgac aacgggactc cggcttgaac cagccaactt
     9421 gcccaggtgt gtctcaagtc gtgaaatctg aaatcatcaa taccagcccg tctcagcgcc
     9481 gctttccagg ctgtgtttgc gtcataccgc atcttcctta ctgttggcgc tttcgttccg
     9541 tctggtttgg tacagctttc cttgtacaca aatacccaac ggtgatgatt cccgatttgt
     9601 tttttcaaaa cgcgacatgc agtatcattc agcgcaacgc cgattgcgcg gtttgattta
     9661 ctctcttccg ggtttatcca tgccacccgg cgctgcatat ctatttgttg ccattcaagg
     9721 ttgatgatgt tcgagcgtct taagcctgtt gccagtgcaa attcaacaac agactttaat
     9781 ggctccggac attcatcaat cagcctttgt gcttcatggg gctccagcca gcggatccgt
     9841 ttattctttg gttgaggcac tttaataatt ggtgccttat ccagcatttt ccattcacgc
     9901 tctgcggctc ttagtagtaa tgccagtcag ttaagcaact gactggctct ttttc
//
LOCUS       DADLSE010000458         4395 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.guided.231, whole genome shotgun sequence.
ACCESSION   DADLSE010000458 DADLSE010000000
VERSION     DADLSE010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.guided.231"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            <1..230
                     /locus_tag="JN692_004526"
     CDS             <1..230
                     /locus_tag="JN692_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HAY7266565.1"
                     /translation="LDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGREAWT
                     LGQGMKKPVAGCYGRLMRWKGGGDVVYMALL"
     gene            complement(262..912)
                     /gene="tetR(A)"
                     /locus_tag="JN692_004527"
     CDS             complement(262..912)
                     /gene="tetR(A)"
                     /locus_tag="JN692_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HAY7266566.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            1018..2217
                     /gene="tet(A)"
                     /locus_tag="JN692_004528"
     CDS             1018..2217
                     /gene="tet(A)"
                     /locus_tag="JN692_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HAY7266567.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(2249..3133)
                     /locus_tag="JN692_004529"
     CDS             complement(2249..3133)
                     /locus_tag="JN692_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HAY7266568.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(3271..3663)
                     /locus_tag="JN692_004530"
     CDS             complement(3271..3663)
                     /locus_tag="JN692_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAY7266569.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3667..4395
                     /locus_tag="JN692_004531"
                     /pseudo
     CDS             3667..4395
                     /locus_tag="JN692_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          846 a         1404 c         1357 g          788 t
ORIGIN      
        1 ccctggacct cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc cgcgccacgg
       61 cccacgagcg gacgccgcag caggagcgcc agaaggccgc cagagaggcc gagcgcggcc
      121 gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc
      181 tgatgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt
      241 gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac
      301 aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc
      361 gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc
      421 ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac
      481 ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa
      541 acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg
      601 tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct
      661 gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt
      721 atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa
      781 gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag
      841 accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg
      901 caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac caacttatca
      961 gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg
     1021 aaacccaaca gacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc
     1081 ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc
     1141 accgcccact atggcattct gctggcgctg tatgcgttga tgcaatttgc ctgcgcacct
     1201 gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt ctcgctggcc
     1261 ggcgctgctg tcgactacgc catcatggcg acggcgcctt tcctttgggt tctctatatc
     1321 gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc
     1381 gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg
     1441 ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct
     1501 ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg
     1561 ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct
     1621 tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc
     1681 atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt
     1741 cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc
     1801 gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg
     1861 ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg
     1921 atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa
     1981 gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg
     2041 gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct
     2101 tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc
     2161 ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg
     2221 tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc
     2281 taggagtgcg gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga
     2341 tgatttgaag cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc
     2401 ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga
     2461 accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat
     2521 tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa
     2581 gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact
     2641 tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc
     2701 cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc
     2761 aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga
     2821 acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt
     2881 agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga
     2941 tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg
     3001 ccggcaacgc ccgcagcatc gcgaccgtca tcggtgagaa gttcggcagg tattgggtgg
     3061 tgacaatgta ggtgctgccc caaatggcag gtgctatcgc tgtgaacaat aaatcgggcg
     3121 tgcgtaatga catgcgatta caagacctcc gctacggcga tgggtttttg agagttgcag
     3181 gcgccgcgat gaccgcaccc tgacttggac tggccctgcc tcgcaccaat ccacctgcgg
     3241 ctatggccgc gatcatgcga ggcccgaaaa ttacacgacc tgtacacgtc cgccgaaccc
     3301 ggaacgcaac agagcttcgt gggcatcccg atccgggtcg tagcagcagt cctggaccaa
     3361 acgcacgtcg tagtccgcat cactagccca ggcgacgctt gaaagaacaa cgccggtggt
     3421 gcttatcccg gccatgacaa gcgtgcttac gccgcgcgtc cgaaggtcgg cgtcgagcgc
     3481 cgtgccataa aagacgctgg ctcgcggaca tgcatagaaa aggtcgcccc gttcgatcgc
     3541 aagcccttcg acgggcagac cggtgcgaaa tcgtccgctc gggagatacg gtgagatttg
     3601 gcgattcgtc gccggcggcg catgttcata ctcttcgccg agcgaaaagt tggggaacag
     3661 cacgggccgg ccgcgcaagg cgtgctggca gccgtgcaga ccctgcgtga gatgaacgcc
     3721 gacaacctgc gcaaggtgcc ggccgatgca cccacggcct tcatcaagcc gcgctggaag
     3781 ccgctggtga tcaccccgga aggcctcgac cggaaattct acgaaatctg cgccctgtcc
     3841 gagctgaaga acgccctgcg ctccggcgac atctgggtca agggctcgcg gcagttccgc
     3901 gacttcgacg actacctgct gccggccgag aagttcgccg cactcaagcg cgagcaggcc
     3961 ctgcccctgg cgatcaaccc gaacagcgac cagtacctgg aagagcgttt gcagctgctg
     4021 gacgagcagt tggccaccgt cacccgcctg gccaaggaca acgagctgcc cgatgccatc
     4081 ctcaccgagt cagggctgaa aatcaccccg ctggatgcgg cggtgccgga tcgggcgcag
     4141 gcgctgatcg accaaaccag ccagttactg ccgcgcatca agatcaccga actgctgatg
     4201 gacgtggacg actggacggg cttcagccgc cacttcaccc acttgaagga cggggccgag
     4261 gccaaagaca ggacgttgct gctgtccgca atcctcggtg atgcgatcaa cctcgggctg
     4321 accaagatgg ccgagtcgag ccccggcctg acctacgcca agctgtcctg gctgcaagcc
     4381 tggcacatcc gcgac
//
LOCUS       DADLSE010000459         2472 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.guided.288, whole genome shotgun sequence.
ACCESSION   DADLSE010000459 DADLSE010000000
VERSION     DADLSE010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.guided.288"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            26..1159
                     /gene="blaEC"
                     /locus_tag="JN692_004532"
     CDS             26..1159
                     /gene="blaEC"
                     /locus_tag="JN692_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7266570.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1248..1781
                     /locus_tag="JN692_004533"
     CDS             1248..1781
                     /locus_tag="JN692_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7266571.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /locus_tag="JN692_004534"
     CDS             complement(1778..2095)
                     /locus_tag="JN692_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HAY7266572.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN692_004535"
     CDS             complement(2271..2417)
                     /gene="ecnB"
                     /locus_tag="JN692_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7266573.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT          632 a          650 c          612 g          578 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaacc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagttct cctacgccgc
     1321 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata ctgactatca
     1981 tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaaa tttggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt acctttatga
     2221 cgatcgcaga aaaaacaaac ggcacgacac tgctgtcgta ccgttattgc ttattgctgc
     2281 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
     2341 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
     2401 attgtcttct tcaccataac gtcttccttt taggtaatga accgccccgg gaatcctgga
     2461 gactaaactt cc
//
LOCUS       DADLSE010000460         1891 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain HUE07
            SAMEA1029907-rid11416373.guided.313, whole genome shotgun sequence.
ACCESSION   DADLSE010000460 DADLSE010000000
VERSION     DADLSE010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029907
            Sequence Read Archive: ERR047433
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942123.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942123.1
            Genome Coverage       :: 339x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:52:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,537
            CDSs (total)                      :: 4,472
            Genes (coding)                    :: 4,155
            CDSs (with protein)               :: 4,155
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 146 of 317
            Pseudo Genes (incomplete)         :: 89 of 317
            Pseudo Genes (internal stop)      :: 120 of 317
            Pseudo Genes (multiple problems)  :: 35 of 317
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029907-rid11416373.guided.313"
                     /strain="HUE07"
                     /db_xref="taxon:623"
     gene            complement(122..1135)
                     /gene="intI1"
                     /locus_tag="JN692_004536"
     CDS             complement(122..1135)
                     /gene="intI1"
                     /locus_tag="JN692_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7266574.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1291..1764
                     /locus_tag="JN692_004537"
     CDS             1291..1764
                     /locus_tag="JN692_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HAY7266575.1"
                     /translation="MKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
BASE COUNT          443 a          516 c          527 g          405 t
ORIGIN      
        1 atgataagtt tatcaccacc gactatttgc aacagtgccc tttccgcaac cggccgttcg
       61 atgcggcacc gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc
      121 cctacctctc actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc
      181 caactttcag cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat
      241 cctgcacggt tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga
      301 gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc
      361 gctgaaaggt ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg
      421 aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact
      481 tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc
      541 accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca
      601 tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat
      661 ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa
      721 gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg
      781 gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg
      841 gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat
      901 gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc
      961 tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     1021 ggacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     1081 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     1141 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     1201 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     1261 ctaaaacaaa gttaacccag gatgagaacc ttgaaagtat cattgatggc tgcgaaagcg
     1321 aaaaacggcg tgattggttg cggtccagac ataccctggt ccgcgaaagg ggagcagcta
     1381 ctttttaaag cattgaccta caatcagtgg cttctggtgg gtcgcaagac gtttgaatct
     1441 atgggcgcac tccccaatag gaaatacgcg gtcgttaccc gctcaggttg gacatcaaat
     1501 gatgacaatg tagttgtatt tcagtcaatc gaagaggcca tggacaggct agctgaattc
     1561 accggtcacg ttatagtgtc tggtggcgga gaaatttacc gagaaacatt acccatggcc
     1621 tctacgctcc acttatcgac gatcgacatc gagccagagg gggatgtttt cttcccgagt
     1681 attccaaata ccttcgaagt tgtttttgag caacacttta cttcaaacat taactattgc
     1741 tatcaaattt ggaaaaaggg ttaacaaagc tatgcaatcg acggcaaaaa gcttcgttcg
     1801 cttcgcgcac tacgcctttt ccgcgattga tagcgacgtt atgtgaatat tgggcactgt
     1861 tgcaaatagt cggtggtgat aaacttatca t
//
